LOCUS       DAFCNS010000001       101956 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFCNS010000001 DAFCNS010000000
VERSION     DAFCNS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 101956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.001"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(553..2166)
                     /locus_tag="LNE23_000001"
     CDS             complement(553..2166)
                     /locus_tag="LNE23_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6929898.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="LNE23_000002"
     CDS             complement(2307..3215)
                     /locus_tag="LNE23_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6929899.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3398..4522
                     /locus_tag="LNE23_000003"
     CDS             3398..4522
                     /locus_tag="LNE23_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6929900.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4749..5666
                     /locus_tag="LNE23_000004"
     CDS             4749..5666
                     /locus_tag="LNE23_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6929901.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5659..6492
                     /locus_tag="LNE23_000005"
     CDS             5659..6492
                     /locus_tag="LNE23_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6929902.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6575..7693
                     /locus_tag="LNE23_000006"
     CDS             6575..7693
                     /locus_tag="LNE23_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK6929903.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7687..9585
                     /locus_tag="LNE23_000007"
     CDS             7687..9585
                     /locus_tag="LNE23_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBK6929904.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9579..10802
                     /locus_tag="LNE23_000008"
     CDS             9579..10802
                     /locus_tag="LNE23_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK6929905.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10888..12822)
                     /locus_tag="LNE23_000009"
     CDS             complement(10888..12822)
                     /locus_tag="LNE23_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6929906.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13048..13704
                     /locus_tag="LNE23_000010"
     CDS             13048..13704
                     /locus_tag="LNE23_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK6929907.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13778..14131)
                     /locus_tag="LNE23_000011"
     CDS             complement(13778..14131)
                     /locus_tag="LNE23_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK6929908.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14323..15252
                     /locus_tag="LNE23_000012"
     CDS             14323..15252
                     /locus_tag="LNE23_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBK6929909.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15263..16099
                     /locus_tag="LNE23_000013"
     CDS             15263..16099
                     /locus_tag="LNE23_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBK6929910.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16347..16721
                     /locus_tag="LNE23_000014"
     CDS             16347..16721
                     /locus_tag="LNE23_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK6929911.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYGVSIKS"
     gene            16779..17905
                     /locus_tag="LNE23_000015"
     CDS             join(16779..17007,17007..17905)
                     /locus_tag="LNE23_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6929912.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            17986..18375
                     /locus_tag="LNE23_000016"
     CDS             17986..18375
                     /locus_tag="LNE23_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBK6929913.1"
                     /translation="MKEKENYPVQHATKKRWTTVRYGNSDHYIHYDEILYLMSMPGYK
                     NKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITSISRVNEIVTFAN
                     GEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            18551..19294
                     /locus_tag="LNE23_000017"
     CDS             18551..19294
                     /locus_tag="LNE23_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6929914.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            19287..20867
                     /locus_tag="LNE23_000018"
     CDS             19287..20867
                     /locus_tag="LNE23_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBK6929915.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(21118..21597)
                     /locus_tag="LNE23_000019"
     CDS             complement(21118..21597)
                     /locus_tag="LNE23_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK6929916.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(21613..22365)
                     /locus_tag="LNE23_000020"
     CDS             complement(21613..22365)
                     /locus_tag="LNE23_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK6929917.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            22482..22552
                     /locus_tag="LNE23_000021"
     tRNA            22482..22552
                     /locus_tag="LNE23_000021"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22514..22516,aa:Gly,seq:ccc)
     gene            22913..23638
                     /locus_tag="LNE23_000022"
     CDS             22913..23638
                     /locus_tag="LNE23_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBK6929918.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            23893..24159
                     /locus_tag="LNE23_000023"
     CDS             23893..24159
                     /locus_tag="LNE23_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBK6929919.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            24156..24587
                     /gene="ruvX"
                     /locus_tag="LNE23_000024"
     CDS             24156..24587
                     /gene="ruvX"
                     /locus_tag="LNE23_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBK6929920.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            24612..24917
                     /locus_tag="LNE23_000025"
     CDS             24612..24917
                     /locus_tag="LNE23_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBK6929921.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(24998..26143)
                     /locus_tag="LNE23_000026"
     CDS             complement(24998..26143)
                     /locus_tag="LNE23_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6929922.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(26204..26851)
                     /locus_tag="LNE23_000027"
     CDS             complement(26204..26851)
                     /locus_tag="LNE23_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6929923.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            27045..27338
                     /locus_tag="LNE23_000028"
     CDS             27045..27338
                     /locus_tag="LNE23_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6929924.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            27382..27693
                     /locus_tag="LNE23_000029"
     CDS             27382..27693
                     /locus_tag="LNE23_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6929925.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            27690..28314
                     /locus_tag="LNE23_000030"
                     /pseudo
     CDS             27690..28314
                     /locus_tag="LNE23_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            28455..28613
                     /locus_tag="LNE23_000031"
     CDS             28455..28613
                     /locus_tag="LNE23_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6929926.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            28614..28976
                     /locus_tag="LNE23_000032"
     CDS             28614..28976
                     /locus_tag="LNE23_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6929927.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            29013..29861
                     /locus_tag="LNE23_000033"
     CDS             29013..29861
                     /locus_tag="LNE23_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HBK6929928.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            29851..31326
                     /locus_tag="LNE23_000034"
     CDS             29851..31326
                     /locus_tag="LNE23_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBK6929929.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            31592..31999
                     /locus_tag="LNE23_000035"
     CDS             31592..31999
                     /locus_tag="LNE23_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HBK6929930.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            32002..32217
                     /locus_tag="LNE23_000036"
     CDS             32002..32217
                     /locus_tag="LNE23_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6929931.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            32221..32601
                     /locus_tag="LNE23_000037"
     CDS             32221..32601
                     /locus_tag="LNE23_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK6929932.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            32735..32890
                     /locus_tag="LNE23_000038"
     CDS             32735..32890
                     /locus_tag="LNE23_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HBK6929933.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            32959..33432
                     /locus_tag="LNE23_000039"
     CDS             32959..33432
                     /locus_tag="LNE23_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBK6929934.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            33429..35123
                     /locus_tag="LNE23_000040"
     CDS             33429..35123
                     /locus_tag="LNE23_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK6929935.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            35089..35274
                     /locus_tag="LNE23_000041"
     CDS             35089..35274
                     /locus_tag="LNE23_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6929936.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            35278..36453
                     /locus_tag="LNE23_000042"
     CDS             35278..36453
                     /locus_tag="LNE23_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK6929937.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            36446..37969
                     /locus_tag="LNE23_000043"
     CDS             36446..37969
                     /locus_tag="LNE23_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK6929938.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            38025..38309
                     /locus_tag="LNE23_000044"
     CDS             38025..38309
                     /locus_tag="LNE23_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBK6929939.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            38296..38631
                     /locus_tag="LNE23_000045"
     CDS             38296..38631
                     /locus_tag="LNE23_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK6929940.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            38926..39552
                     /locus_tag="LNE23_000046"
     CDS             38926..39552
                     /locus_tag="LNE23_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6929941.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            40294..41130
                     /locus_tag="LNE23_000047"
     CDS             40294..41130
                     /locus_tag="LNE23_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBK6929942.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            41194..41625
                     /locus_tag="LNE23_000048"
     CDS             41194..41625
                     /locus_tag="LNE23_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6929943.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(41687..43009)
                     /locus_tag="LNE23_000049"
     CDS             complement(41687..43009)
                     /locus_tag="LNE23_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBK6929944.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(43163..44803)
                     /locus_tag="LNE23_000050"
     CDS             complement(43163..44803)
                     /locus_tag="LNE23_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBK6929945.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            45136..45606
                     /locus_tag="LNE23_000051"
     CDS             45136..45606
                     /locus_tag="LNE23_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBK6929946.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            45828..46931
                     /locus_tag="LNE23_000052"
     CDS             45828..46931
                     /locus_tag="LNE23_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK6929947.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            47215..47895
                     /locus_tag="LNE23_000053"
     CDS             47215..47895
                     /locus_tag="LNE23_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK6929948.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            47985..49019
                     /locus_tag="LNE23_000054"
     CDS             47985..49019
                     /locus_tag="LNE23_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBK6929949.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            49033..49368
                     /locus_tag="LNE23_000055"
     CDS             49033..49368
                     /locus_tag="LNE23_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6929950.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            49352..51832
                     /locus_tag="LNE23_000056"
     CDS             49352..51832
                     /locus_tag="LNE23_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6929951.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            51888..53447
                     /locus_tag="LNE23_000057"
     CDS             51888..53447
                     /locus_tag="LNE23_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6929952.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            53444..54808
                     /locus_tag="LNE23_000058"
     CDS             53444..54808
                     /locus_tag="LNE23_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6929953.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            54978..56048
                     /locus_tag="LNE23_000059"
     CDS             54978..56048
                     /locus_tag="LNE23_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6929954.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            56063..56731
                     /locus_tag="LNE23_000060"
     CDS             56063..56731
                     /locus_tag="LNE23_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6929955.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            56751..57329
                     /locus_tag="LNE23_000061"
     CDS             56751..57329
                     /locus_tag="LNE23_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6929956.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            57730..58641
                     /locus_tag="LNE23_000062"
     CDS             57730..58641
                     /locus_tag="LNE23_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK6929957.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(58729..59646)
                     /locus_tag="LNE23_000063"
     CDS             complement(58729..59646)
                     /locus_tag="LNE23_000063"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBK6929958.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            59848..60285
                     /gene="zapA"
                     /locus_tag="LNE23_000064"
     CDS             59848..60285
                     /gene="zapA"
                     /locus_tag="LNE23_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBK6929959.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            60344..60892
                     /locus_tag="LNE23_000065"
     CDS             60344..60892
                     /locus_tag="LNE23_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBK6929960.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            61007..63367
                     /locus_tag="LNE23_000066"
     CDS             61007..63367
                     /locus_tag="LNE23_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBK6929961.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            63454..63768
                     /gene="trxA"
                     /locus_tag="LNE23_000067"
     CDS             63454..63768
                     /gene="trxA"
                     /locus_tag="LNE23_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBK6929962.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            63950..65728
                     /gene="uvrC"
                     /locus_tag="LNE23_000068"
     CDS             63950..65728
                     /gene="uvrC"
                     /locus_tag="LNE23_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBK6929963.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            65873..66772
                     /locus_tag="LNE23_000069"
     CDS             65873..66772
                     /locus_tag="LNE23_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6929964.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            66765..68027
                     /locus_tag="LNE23_000070"
     CDS             66765..68027
                     /locus_tag="LNE23_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6929965.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            68188..70086
                     /locus_tag="LNE23_000071"
     CDS             68188..70086
                     /locus_tag="LNE23_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBK6929966.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRISSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(70416..71135)
                     /locus_tag="LNE23_000072"
     CDS             complement(70416..71135)
                     /locus_tag="LNE23_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBK6929967.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            71472..71804
                     /locus_tag="LNE23_000073"
     CDS             71472..71804
                     /locus_tag="LNE23_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6929968.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            71791..73794
                     /locus_tag="LNE23_000074"
     CDS             71791..73794
                     /locus_tag="LNE23_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBK6929969.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            73813..74283
                     /locus_tag="LNE23_000075"
     CDS             73813..74283
                     /locus_tag="LNE23_000075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBK6929970.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            74304..74888
                     /locus_tag="LNE23_000076"
     CDS             74304..74888
                     /locus_tag="LNE23_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBK6929971.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            74903..75889
                     /locus_tag="LNE23_000077"
     CDS             74903..75889
                     /locus_tag="LNE23_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBK6929972.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            75879..76190
                     /locus_tag="LNE23_000078"
     CDS             75879..76190
                     /locus_tag="LNE23_000078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBK6929973.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            76212..77993
                     /locus_tag="LNE23_000079"
     CDS             76212..77993
                     /locus_tag="LNE23_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBK6929974.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77986..79362
                     /locus_tag="LNE23_000080"
     CDS             77986..79362
                     /locus_tag="LNE23_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBK6929975.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            79369..80004
                     /locus_tag="LNE23_000081"
     CDS             79369..80004
                     /locus_tag="LNE23_000081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBK6929976.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            80034..81389
                     /locus_tag="LNE23_000082"
     CDS             80034..81389
                     /locus_tag="LNE23_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBK6929977.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(81581..82975)
                     /locus_tag="LNE23_000083"
     CDS             complement(81581..82975)
                     /locus_tag="LNE23_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK6929978.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            83067..84038
                     /locus_tag="LNE23_000084"
     CDS             83067..84038
                     /locus_tag="LNE23_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBK6929979.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            84035..84817
                     /locus_tag="LNE23_000085"
     CDS             84035..84817
                     /locus_tag="LNE23_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBK6929980.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84986..85357)
                     /locus_tag="LNE23_000086"
     CDS             complement(84986..85357)
                     /locus_tag="LNE23_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6929981.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            85491..85934
                     /locus_tag="LNE23_000087"
     CDS             85491..85934
                     /locus_tag="LNE23_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBK6929982.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85977..86786)
                     /gene="yidA"
                     /locus_tag="LNE23_000088"
     CDS             complement(85977..86786)
                     /gene="yidA"
                     /locus_tag="LNE23_000088"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBK6929983.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(86805..88172)
                     /locus_tag="LNE23_000089"
     CDS             complement(86805..88172)
                     /locus_tag="LNE23_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6929984.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(88156..88986)
                     /locus_tag="LNE23_000090"
     CDS             complement(88156..88986)
                     /locus_tag="LNE23_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBK6929985.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            89149..89586
                     /locus_tag="LNE23_000091"
     CDS             89149..89586
                     /locus_tag="LNE23_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBK6929986.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            89662..90282
                     /locus_tag="LNE23_000092"
     CDS             89662..90282
                     /locus_tag="LNE23_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBK6929987.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            90471..90977
                     /locus_tag="LNE23_000093"
     CDS             90471..90977
                     /locus_tag="LNE23_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBK6929988.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            90994..91893
                     /locus_tag="LNE23_000094"
     CDS             90994..91893
                     /locus_tag="LNE23_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBK6929989.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            91980..92864
                     /locus_tag="LNE23_000095"
     CDS             91980..92864
                     /locus_tag="LNE23_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK6929990.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(93056..93127)
                     /locus_tag="LNE23_000096"
     tRNA            complement(93056..93127)
                     /locus_tag="LNE23_000096"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(93092..93094),aa:Arg,seq:ccg)
     gene            93187..94521
                     /gene="rpoN"
                     /locus_tag="LNE23_000097"
     CDS             93187..94521
                     /gene="rpoN"
                     /locus_tag="LNE23_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBK6929991.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            94670..95761
                     /locus_tag="LNE23_000098"
     CDS             94670..95761
                     /locus_tag="LNE23_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBK6929992.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            95802..96803
                     /gene="gap"
                     /locus_tag="LNE23_000099"
     CDS             95802..96803
                     /gene="gap"
                     /locus_tag="LNE23_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK6929993.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            96913..98103
                     /locus_tag="LNE23_000100"
     CDS             96913..98103
                     /locus_tag="LNE23_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBK6929994.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            98207..98962
                     /locus_tag="LNE23_000101"
     CDS             98207..98962
                     /locus_tag="LNE23_000101"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBK6929995.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            99078..100376
                     /gene="eno"
                     /locus_tag="LNE23_000102"
     CDS             99078..100376
                     /gene="eno"
                     /locus_tag="LNE23_000102"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBK6929996.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            100775..101914
                     /locus_tag="LNE23_000103"
     CDS             100775..101914
                     /locus_tag="LNE23_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBK6929997.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        33885 a        17402 c        21000 g        29669 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac ttgaggagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
     3241 gctttctatt tataattaat gactattata acaaaaatga aaagccaaaa cgacaagtaa
     3301 aatatttttt tatataaata tttgtgttac taattctgtt aatatacata agtgaaagcg
     3361 ttttatataa gaacttgaaa agaaagaagg gatagacatg aaaaaaagat acaaattggt
     3421 gatagctttg ctaaacatta ccctattatt cagcttaagt gcatgtggaa aaactaacga
     3481 agaagtaaca gcaaacatcg cagatccaat gataaaaaaa gccactacat tagaagtata
     3541 tgtcgcaaac ggcaaactga caaactatgt tgaaaatgct gcaaaaatct ataaccgcaa
     3601 aactggagca aatattgact taaaaattgt gaatgtagct tcgggaacag caacagtaca
     3661 aatgatcacc ccaaaattgg taacacacga agatatgccg gatattatct ccatggcaga
     3721 cacttcagca gcaggtgtgc tggaaaaatt tcaagatgct ttttatagtg ctgatgaata
     3781 tggattttat gacaaatatg gcgatcagtt ttatgatcaa aaattgaata ttttgcgtca
     3841 acaaacgagt aatgaaacga ttattccttg ggctagtgat tttaccccag cactttgtta
     3901 ttatcaacca aaaaaatttg ctgaaatagg tgtagatttt gaagaaatca gttcttggga
     3961 agaatatatc caggtggcaa aagcattaaa agaaaaaact ggtagttttg gaattgcctt
     4021 gccagaatct ggggaccaag aattgtttat caacatgcta gcccagcaga atcaatcttt
     4081 gcttgatgaa gaaggaaata tccgtctgaa tactaaagaa gcgaaacatg ctgcaaagtt
     4141 aatcaaagaa atgatcgact cagatattgt acatttctac ggtgcacaag atgctgagaa
     4201 agcatttcaa gaatctgcaa tgtttgttgc tggcggctgg tatgctacga atatgtcttt
     4261 gaattttcct gatgcatctg acaaatggcg catggctcca ctgattcctt tttcaaaaga
     4321 aaatccagga aaaggtgcag tatcaggagg atcaagtttt tatgtcccta aagatgcaaa
     4381 aaatccacaa gtggcacaac aatttcttac atttatgctg acagatgaag aatgcttagc
     4441 caacgcactg gagctgggcg tggctacatc taactcattg gcctatcaaa cagaagcagc
     4501 tgagaaaaag tttgactact aaggaggaca aaaatactat gaactattga ataaaatcaa
     4561 tgaaaataca tcaaatactg ttttaccagc cacttattca gatgctacgt cttatttgac
     4621 taccgctgca tataactatt ggcaaacagg tgattttgaa aattcatatc taaaagaagc
     4681 agaaaatttt gctcataagt acggcgtaaa attaactggt gattaaaaaa tattaggagg
     4741 acttaacgat gatgcaatct tcgttaccaa gcaagacaat aaagaacagc aagaaaaaag
     4801 caacagatac gtctcataaa aaaagtgctt atggattggt cacacctttt gttattttct
     4861 ttattgtgac gattttgatt cccatcatta tctcgtttgc ttttagtttt atgaaaattg
     4921 gctactcatt tgaatttgtt ggatttgaca attatctagc tatcttccga gatccattgt
     4981 ttattaaatc ttacacgaat gttttgatta tcatggcagg atcaattcca ataacgatgc
     5041 tattagctat ttactttgct gttttattga attcagcaca gttaaaagga aaaggatttt
     5101 ttagaactgt ctattatatt ccaaccatta catcaacggt ggctgtagct tccgtattca
     5161 tgactttttt caatccgact gggttattca atggattttt gaatgcgcta ggaattaacg
     5221 gtgtacagtg gttaacaaat cctttttgga tacgaatatc aatgatcatc acgatgattt
     5281 ggcttagtgt aggatataac actattttat ttttagctgg tcttcaaggg atttctaaag
     5341 agatatacga aagtgctgaa atagatggag catcgaaatt tcggcaattc tttttattaa
     5401 cgatccccat gctaaagcca atcattttaa tggccacagt tttagccact atcaatggct
     5461 tgggtaattt caatatccca aatatattct ttgggacttc caatggacca gaaaattcag
     5521 ctttggtcgt aggagtcaat ttatataaaa caagttttga aatggttgat tttggaaaag
     5581 cctctgctat tgcctggacg atggtgtttg tatctgttat attatcggct attcaattta
     5641 aattcggagg tgataaaaat gactaaacaa aagatacaaa gcagaatttt tacgtacgtc
     5701 attattgtgt ttggtgcctt aatatttttg atgccatatt tatatatgat tgtagcttca
     5761 acgcaaagta atcaagaaat tttaagtggc ggaatcaatt tcaagtttgg ggatatgcta
     5821 ttaaaaaact ggcaagccat catggcgaga tataattatc ccttgattat ttggaattcg
     5881 attgttattg ctgtaatcag tacagtttta gctacagcca gttctacaat ggcgggttat
     5941 gcattagcaa agtatcgttt tatggggaat aaactgttgt tcaatacagt tatgctatct
     6001 aggatggtgc ctttttttac cacattgatt cctttatttt acattatgag tcgtttagga
     6061 ttatcgaata cttatgcagg tgtgatcatc ccttcgattg ccagtacaac ttcagtgttt
     6121 atgatgcgac aatatagcag tcaatttcct acagcactga tggaggctgc cagaatcgat
     6181 ggtgccaatg agtgggtgat ttttacgaaa atcgctgtac cgattttatc acctacgatt
     6241 attactagtg gtttacttat ctttatggga agttggaatc aatatctatt tccattagtc
     6301 atgctttcag ataaagataa atatacggtt cccttaatta tcaaaaatat gacggtttct
     6361 agtacaggtg agccaattaa ttatggcgca attatgctaa ttttagcaac ttcagtgatc
     6421 ccaatggtct tgatttatgt gtgggcgcag tctaaattta aggagaatga cattggagct
     6481 gcaaataagt aaaaaaaata acgaatgtta accatctagt tcgttctagg agcaactcca
     6541 cattcataaa aattaaaaga atggcgggat atccatgaat gaacagacga aggaatggtc
     6601 aagaaaaatt ttgaatgtag cttctgaaaa gatagatgcg gaattagaca gaatggggaa
     6661 taaaattcca tatatttctt acaacggaaa atatacagac gtaataaagg acagaggaat
     6721 cgactggtgg accaatggtt tttggagtgg tattttatgg cagctctttc acaagacaaa
     6781 acgagaaaag ttcaaagagg cagcaataca acaagaacaa ttattagaat ctgccctgta
     6841 ccatttttca gacgttcatc atgatgtagg ttttttattc ttaccatcgg ctattgcaca
     6901 ttatcaacag tctcaaaaca aacatagtcg acaactaggg ttacatgcag ctacggtact
     6961 tgctggacgc tataatattt cagggaacta tttagtagca tggaatgacc ataaacctgg
     7021 ttggatgatt attgatagta tgatgaattt accattactg tattgggctt cgagggaaac
     7081 tggtgatccg aggttctcta aaattgctcg gcagcatgca gatacaacag ctaagtatct
     7141 agtgaggaaa gatggatcaa ccgggcatat cgcagaattt gatgcagaat cagggaaata
     7201 tattcggtta cttggcgggc aaggatacaa tgagaattct tgttggagca gaggaaatgc
     7261 atgggcttta tatggattcg cgttaagtta tcgttacaca aaaaatccgt actatctagc
     7321 tatagcagaa aagatagcag taaattttat tcagaaaatc gaaaaaactg gttatattcc
     7381 tctcattgat tttgctgtaa aagattcgaa tattgatacc agtgctggag tatgcgctgc
     7441 atgcggtctt ctggaattag caggatatgt ttcctctgta cgatcaaaaa tgtatcatga
     7501 agctgctatc aatatactcc aaaagacaga aaagcactat gcaaattgga atatggatga
     7561 agatgggatc attggaggcg gagcagaagc ataccataga gaactgactt ataatgtgcc
     7621 actgatctat tctgattatt tttttattga aggcttactt cgattagaac agtcggaatt
     7681 agctatttgg tagtaaaaag aggagaaaat acaatgaaac atccagtacc actattaagg
     7741 accagacagg attactttga cttatttaaa aagataaatc aaccactcca gccgttttat
     7801 cgagcaagtg gcgcacaaat tgattatata catcaagggg taggatatgg aaataaaatt
     7861 gcaggaatgg aaggattctc tcgggtttta tggggagcgg ggcccgcaat tgaccaatta
     7921 gatgaacgat ggttgaaatt aattctatca ggaataaaag ctggaacaga tccttcccat
     7981 cccgattatt ggggagaaat acatgaaaga gaccagcgta tggtagaaat gcccgcaatg
     8041 ttattagcgt tatatcatca tgaacaatta ttatggaaaa aattagatac aggagaaaaa
     8101 agccagatta ttcattggtt ctcgcaaatt tttgaatatg aatgtgcaga tggtaattgg
     8161 caatttttta agataattgt cggaacaatt atccaaaaat ttggttatcc agttaaagaa
     8221 tcagcactga aagaagcatt tgataatata gaaaattgtt atcttcaaaa tggctggtat
     8281 cgagacagtt caagaggtcg ccaagattat tataatcctt ttgcatttca ttattatggt
     8341 ttgatttata gcgtactaga accagaaaag cccattagcc agatatataa gaaccgagct
     8401 attgattttt ctagagacta tattcatttt tttgcggaag atggtgcaaa tgtgcctttt
     8461 ggtcgaagca tgatttatcg ttttgcagtt tcaagctttt ggtcagctat gttgtgggcc
     8521 gatcttttgc cttttaaatt aggtgaaatc aaggggttag tcaatcggaa tataagatgg
     8581 tggatgcaaa aagaaatatt tgatgaaaaa ggtatcctga atattggtta tacctaccct
     8641 caattgacat tgactgaacc ctataatagt acgttatctc cattatggct gaataagatt
     8701 tttttattgt tggcgctacc tgaagaccat gagtattgga cggctaaaga aatgcctatg
     8761 cctattcgtg aaggaactaa acttctttca gaagccaatt tgctcataca acatgataat
     8821 ggacatacat tttttttgaa tgcgggacaa ttagggccaa atttccatac attgaccaat
     8881 gaaaaatatt taaaatttgc ttattctagt caatttggtt tttcgatacc gcgagcgaat
     8941 caattgaaag cagaaatggc aatggattca atgctaggga ttcagcgagc ggacacatat
     9001 atcacgacca gttttaagaa tgagagcatc aaaacctatg gacagttcat cgttcgaaat
     9061 aatgtacgag acattgtatg taagaaagag gtacttgcct ctacatggga acctacttta
     9121 caggcaaaaa tcagaacttg gcttattcct tttgaaggct ggcagattcg cgtacacaaa
     9181 gtagaattag atacagaata tgttctatat gagacagggt ttgctattga atgttctcct
     9241 gagaaagaag gaataatcat agaagaagac aaggaaaact atagggtgag tgaagctggc
     9301 ttttcaggta ttatctgttt aagtgacgat actattaaac gaaaatccac agctattatg
     9361 ggtttgccca atacaaactt gatgacatgg gaaaacactt ttattcctgg attagagggt
     9421 acatttggaa aaggaaccca ttggctcgga acaggagtgg ttgctcatca agatcgggat
     9481 tatagtttag aagtatggaa caatcgtccc aaaattgatt ttgatgggac aaccgggcta
     9541 actatccaaa ataaaaacaa taaaaaaaga ctaaaattat gctaaaggct ggtggggaaa
     9601 tgcttgataa agtgatgttt gaagagataa tacatccaga aaaatggata aatgaatgtt
     9661 ataaagaatt aatggaaaaa gaatggaata atgagatcga gcgttgtcgc agacacacgg
     9721 agaggttaat caaacgtatc agtaacagtc agtatcaatt cccaagcgag tgtcctataa
     9781 aaggacgtta tccattaaga gacaatattg gttggacaga aggattttgg acaggaatta
     9841 attggttgtt atttgagaag acgggtgatc ctttatttca acaaacagct gaaaaagcag
     9901 atatagattt tatccacagg attgactgtc aaattatcac taatcatcac gatcttggtt
     9961 ttttgtattc tttatcaacc gtagcagatt ataaaataac gggaaatcga aaaatgaagg
    10021 aagcttcaat tgatgctgca gaaacattac ttcagcgttt ttgggagaag gcaggagtta
    10081 tacaagcttg gggagatcct agtgatcctt cacaacaagg tagaatgatc atggattgtt
    10141 gcatgaatct tccattattg tattgggcga gtgaagaaac tggaaatcaa aaatataaaa
    10201 aagctgctca ggctcatatt caaaatgcag cagattattt agtaagagaa gatggttcaa
    10261 cttatcatac attttatatg gatgtcaaag atggaaaacc aagatatgga agtacgcatc
    10321 aaggttatag tgataattcc tgttgggcta gaggccaagc atgggggatt tacggttttg
    10381 cattaagtta tcgttatttg aaagaacaaa aatattttga tttaagcaaa atattagcca
    10441 attattttat gaatcggttg ccagaagata ccatttgtta ttgggatttg atctttgatg
    10501 attctagcag agaagaaaaa gatacatcta gtatggtgat catggtttgt ggattattag
    10561 aattaataga atcaggtcta ttagacaaaa cagaaaagaa cttataccag tctgctgttt
    10621 ggaaaattat gcgacaattg aaagaaagat atgctacaac ggaagaagaa gatggctttt
    10681 taacacagtc tgtttataat aagcccaaaa ataatggggt aaatacacct tgtatttggg
    10741 gtgattacta ttatttagaa gctttagtga gaatgactag taattggtct tcttattggt
    10801 gaaaaaggta aatatggaat ttttgtttga taaaataaaa aaagaaaaac caaaacagca
    10861 tgaatggttt ctgttttggt tttttcgtta attttcatat tcttctaatt ccaagcttag
    10921 ttcttcccat tccgtcaatt ttgtttcttg atcttgtcta gcagcttcta aatcttggtt
    10981 cagctcattg agttttacgt gatcttccag gttttctggc gcgaccattt cttgttccaa
    11041 ctgatcgatc cattcatcga tccgcgccat ttcttcttca acctgagtaa tttttcgttg
    11101 aaggctacgg ataagctttt gctgttcttt gctttggtaa taatcagatt tgttcgtggg
    11161 agcaggagca gattctggct catcttgtgc caacaattcg gcgatttctt cttcttcctg
    11221 ctttttctct aaataataat catagtctcc tagatagact ttgctgccat tttcagagag
    11281 ttcgacgact tttgtcgcga tccgattgat gaaatatcgg tcatgggaaa caaaaagcag
    11341 tgtgccgtca taatcgatca atgcattttc taatacttct ttgttgtcaa tatctaaatg
    11401 gtttgttggt tcatccaaaa tcaaaaaatt ttctttgttc atcgctaact tggctaaagc
    11461 aacccgtgct ttttcaccgc cacttagcag agggattggt ttttctacat cttcgccaga
    11521 aaataagaaa gcacctaaag cacttcggat ctctttttct ggagtggtag gatgctcatc
    11581 ccacaattct gctaggatcg tcttgtttgt atgcagatca gcttgacctt gatcgtaata
    11641 gccgatggtg acatttgtcc ctagcgtttc ttttccacgg ataaaaggga tctggtgaat
    11701 gattgatttc agcaacgtcg atttcccgat accattcgga cctactagag caatcgcttc
    11761 ttgacgacgg atatctagat tgattggttc agataaggtg acattatcat atccgatagc
    11821 ggcatcttcg atttgcaaga ctacatttcc agaaggcttt tctatactga atagaaagtt
    11881 tgcttgtttt tcatcccctt gaggacgatc cagccggtcc attttttcta atgtctttct
    11941 gcggctttgc gctcgttttg tggtagaggc gcgaacaagg ttccgagcaa cgaagtcttc
    12001 taatttatgg atttctgttt gctgcttttc ataagccttc cattcacttg ctaactgttc
    12061 tgctttcaaa tctagatatt tcgaatagtt acctttgtaa tggcgcattt ttcttctgct
    12121 taattcatag acttcattga ctactttatc taaaaaataa cggtcatggg aaacaatcaa
    12181 taatgcacca gtatagcttt gcagataatt ctcaagccag gaaagtgtct cgatatctaa
    12241 atggttcgtt ggttcatcta gaatcaaaat gtcagggtgt tgcaaaagca tgcgagctaa
    12301 agctaaacgt gtgcgctgtc cgcctgataa agtatcgatt ttctgatcat aaaatgaaga
    12361 atcgaattta aacccatgca atacggaacg aatttcattt tcataaccat agccgtttcg
    12421 atctgaaaaa tcgtgttgca gccggtcgta ttcttttaaa taagaactat aagcaggtgt
    12481 gtctggcgta ccttcgctaa tgaaaatctc tagctcgcgc atccgctctt ccatttgtct
    12541 aacttgttca aaagctttca gcatctcgtt ccaaactgtt tcattggaat caagtcctgt
    12601 gtcctgagcg aggtatccga gagtcgctgt tttattttta gcgatctttc cttcatcagg
    12661 tgcttcaatt ccagcaatga tctttaataa agtcgatttt ccagcgccat ttctgccaac
    12721 taaagcgaca cgagcgcctg tactgatttc taaatgtatg ttttcaaata agacttctgc
    12781 accaaaatgg cgagcaatct gattggcttg taataatatc atggaatcct cttttctact
    12841 ttgtgaattc tcctcaaata aaaaactatt tttcaattag tagtctacca taaattccat
    12901 tatactgcga gatttgcaga aatgataaat aaatgaataa agaataaaat ttcacaatat
    12961 ctgagcaatg gaattgattt tttcctataa atactgctat gatggagaaa tcaaagatgt
    13021 aattagacaa tacgtggagg tcatttcgtg aaagatcata ttattccaaa ggcgacagct
    13081 aaaagactgc ctttgtatta tcgctattta aggattttaa ataatgctgg aaagaaaaaa
    13141 gtgtcttcaa ctgaattaag tgaagccgtt caagtagata gtgcaacgat ccgtcgagat
    13201 ttttcttatt ttggcgaatt aggaaaacgt gggtatggtt atgatgtaga agatcttatg
    13261 cacttttttg caaagacatt aaacgacgat caattgacaa atgttgcgct gatcggtgta
    13321 gggaacttgg gaagtgcttt actaaaatac aaattccatc aaagtaatag catacgtatc
    13381 agttgtgctt ttgatgtgaa tgaggaaatc gttgggcgca tcgtagacgg tatccccgtt
    13441 tatccaatgg aagatatgtc agaacaaatc aagatccaac aaatcgaagt agcaatttta
    13501 acgatacccg ctgaaagagc acaagaagta gtcaatcagt taacggaagc aggaatcaaa
    13561 ggaatcttga actttaccgc tgctcgattg aatgctccgt cagatgtatt gatccaaaat
    13621 gttgatttga cgaatgaatt gcaaaccttg atctatttct tgcattctga cgcaaatgcg
    13681 cattccgaaa atacagcaga ataaaaataa aaagcgattg aacaaatgaa ggcaaaactt
    13741 tcacttgttc aatcgctttt ttctgtttgg acaacgatta ttttgttcgg aagatttctt
    13801 cataagaaag ggaatcatct tctgctgtca gttcatctag tagataacgg atttttttaa
    13861 agtcgatgaa ccgtgcttgc cgaaagactt cttttccttg atgaaaaatc aggacaactg
    13921 gcgcagtcag cacttcaaat ttactagcta cttctggtgt ttcaaatgca tccaaatgaa
    13981 gtgccggaat atcataatgt gtgagaagtt cctctagtcg tggacgaaca gcgtgacaaa
    14041 cactgcaatt aggcatcgaa agataaatga cggcaacgga tgactgttgc agtatgtctt
    14101 gaacttcagt caagttagtg gtttgtttca tttttattct cccttttatg atagaagtga
    14161 tatgtatgag accttaattt aatgggaata acagaaaaag tcaattgaag tgctttggta
    14221 aaaaataaga gttgtgtaaa aatttacaga gcaacttgtt ttttttgaat aaaagcgtat
    14281 atttgcaaag cgaatgaact agaaataaaa aggaggaaac tcatgttttc ttttattcca
    14341 aattcggttt tgcagatgag tacgattttc ttatcgatcg ttattgaagc attacctttc
    14401 gtattattag gctgtcttat ctcaggcgcc ttacaagtat ttttgacccc agaacgtgtg
    14461 aagagatggc ttcctgaaaa tcgtatcttc tctattttta ctggttcgat cttaggattt
    14521 ttctttcctt catgtgaatg cggcatcgta ccgatcgttc atcaatttgt taaaaaaggc
    14581 gtaccagttc acacagcgtt tgcttttatg ctgactgctc cgatcatcaa tccaatcgtg
    14641 attttttcca cttttatcgc atttggcaat tcttggcaga tggcaagctg gcgggtgata
    14701 ggcagctttt ttgttgccct tgtgattggt gtctggcttg cctattttca aaaagacgct
    14761 gtattgaaag aaaaaatcac gtctaaagta gcagaaaatc accatcatca tgtccatgaa
    14821 gaagaacaaa gtgagaagaa tgaaccaaaa aatcagctga tccatcaaac tgggcatgtg
    14881 ttgacacatt caatcgacga attttttgat acaggtcgtt atttgatcat tggcggatta
    14941 atcgcagctg cgatgcagac ttatttacca actagaatta tgctaacgct cggctcaaca
    15001 aagattttag ccattatcat catgctgtta ttggccttta cgatgtctct ttgctcagaa
    15061 gcagatgcgt ttataggatc ttctttgtta agtctgttcg gcacagcacc tgtagctgcc
    15121 tttttggtct ttggcccaat ggtcgatatc aaaaatctac tgatgatgaa acgatatttt
    15181 acaggacgct ttattttatc gctgatcgga ctgattgccc ttagcgttat tggattcaca
    15241 ttatttattt aggagggaaa atatgctgcg gtttttgatt ttatctgggt atttcgaatt
    15301 gatgatgtac ttgcaagttt ctggcaaact cgatcaatat atcaatgtcc attatcgcta
    15361 cttagctatt ctttcaatga ttttatctgc tcttttggct ttagttcagc tgtatttgtg
    15421 ggtcaaaaac ggaaaagcag aagaaaaggc acacgagcac gatcatgacc atgggttgtc
    15481 aaaaccttat caacgtggaa tcgcttatgt actgctagct ttgccagtaa tcgtggggac
    15541 gttgtttcca acagttagtc tcgacactac gatcgtagaa gccaaaggat ttaatttccc
    15601 agtaagtaaa gaatcagtag gggatccgga gatgcagaca caatacttaa agccggatac
    15661 cagtatttat ttcaacaaat cagactactt agaccaaatg aatgagttga aagaaaagta
    15721 tggggataag caaactatca agatcacaga tgaaaactat ttagaagtta tggagctaat
    15781 ttataattac cctagcgaat tcattggcaa aaaaatcagc tatgaaggat ttgtttacca
    15841 aacaccgaac gatagcaata cagatatctt cttgttccga ttcggcatta tccattgtgt
    15901 ggcagattca ggagtctttg gtttgttgac gcatatgccg gaaggagtaa cggttaaaaa
    15961 tgatgaatgg tataaaatag aaggaaccat ccaatcagac tattatcaac cattcaagcg
    16021 tgaaatccca tctgtagttg ttacaaacgc tgagaaagtc tctgctccga aaaatcagta
    16081 tgtgtatcgt tctttttaaa agaacaaaat aaataaacat agctcagaca gctgacacgc
    16141 aaggacgtcg gttgtctggc tttttttgca tgatgtattc atttgtttga tatttatttt
    16201 agaagcagca tcctgaaagt atttgatatc ggtttcaaag gtgttggaaa agattgtgaa
    16261 gagaattaca atttattcct aacatgtatg ttgaaaagtt gtatgaaatg attttgatag
    16321 taaaatatcg agtggaggta ctttttatgg taacgcgtat atttattttg gatgatagtt
    16381 tggtttttga aaaaatgcta gtagaaattt tggaagaatc cagaatttta ttaagtcctt
    16441 ggaaatttga gatcgttcag ggattgaacg ttatacaatt tgttgaattc attaaaaata
    16501 atgaaatcta ttcctcagat atttttttcc tagacatcga tttgaacgcc tattatagcg
    16561 gtatcgaact aggcgaaatg cttagagaaa agaatgaaaa gtgtaaaatt gtttatctaa
    16621 cgtctcatcc gaataaagca ataactgtta ttaatcgaaa gattacgcct agtggttatt
    16681 tgataaaaga attcacttat ggtgttagta tcaaatctta gacagctttt cgtagagaca
    16741 agaataaatt aaacttatct acgagaggat gaatttttat gacccgatat gaagaaaatt
    16801 ttaaacaaat gattgttgaa ctgaatcaaa ctggacgttc tgttcgaggg ttagcgaaag
    16861 aatatggctt atctgaagca acgatttaca aatggaagaa tttatattta cctgatcagt
    16921 ccacaggact gactggaaaa gaagtagctg aactgagaaa agaaaatgct cgtttaaatg
    16981 aggaacttga aatcttaaaa aaagccgcag ccatattctc tcggaaaacc taaattcgct
    17041 tgttcaattt attgaaaaat ggtgtaagga ttacactgtt tctttgttat gtcggttatt
    17101 agaaatccct agaagtgtct attattttta taagaataaa ccgttgacag ctacagaaat
    17161 cagaaataat aagttgaaaa agaagatttc aacaattttt tttacgaata aacaacgcta
    17221 tggtgccaca aagatccatc aagttttatt aaaagaaggg atttcagtat ctcttaaaca
    17281 tgtccaaaag ctaatgaaac aattaaattt aaggtcgatt gtagttaaaa aatatagacc
    17341 tcaaaggtct aataaaccga tcatttcaaa agagaatctc ttaaatcagg acttttctac
    17401 tgaaacgatc tgtgagaaat gggcagctga cattacgtac attcctacta agaaaaatgg
    17461 ttggtgttac ttatcttcaa tcatggattt acacacgaaa aaaattatta gttatacatt
    17521 ttcaaaacga atgactgtgg attgtgtcat tcaaacattg aataaagcaa aaatacatta
    17581 tcacattcca gaaggaatga ttctacacac tgacttgggc agccaataca cagcaagaga
    17641 agtggaacaa tggcttaaaa ccaacaaaat aaggcattct tatagtagaa aaggaacacc
    17701 ttatgataat gctggaatcg aatctttcca tgcctcattg aaaaaagaag aagtctacac
    17761 gactagctac tcagattttg aagaagcaaa tcgagcgcta tttagctaca ttgaaggatt
    17821 ttataaccga aatcgaatcc atagctcgat tcactatcta accccacagg aatttgaaga
    17881 attagcaaaa gaaaaaatgg cttaaccgtc tctactaaaa tgtgtccaag atattgactc
    17941 aaatccactt atgaaaaaaa taaaaaaagc atccaaagtt acttattgaa agaaaaagaa
    18001 aactatcctg ttcaacatgc tacgaaaaaa aggtggacaa ctgtccgtta tggtaatagc
    18061 gatcactata tccattacga tgaaatcctt tatctaatga gcatgccagg ttataaaaat
    18121 aaaataggcc tagtattgaa agatgaagag attctcgtaa acggcacaat taaaaaatat
    18181 aaaaaatcac tcgaatatga gtatttatgt aaagatttaa aagcttatat cattaatact
    18241 gaaaacatta catctattag cagagtaaac gaaatagtaa catttgcaaa tggagaacaa
    18301 ctagagatgg gaaagagaag tattgataaa ctaaagaatt ttatgcaaga aaagatagaa
    18361 aatgaatcat tttaaagaaa acaaagactc atatcttacc tgttagaaga gtgttttcct
    18421 gtagaagaga acaacccaag tagcagccta gtcgtttcta tccttttttg ttatgcactc
    18481 tttttgacat ttactccacc aataacgaag ggtacaataa aaagtaataa caaacaaaag
    18541 gagtacagaa atgattgaat tcatcaatgt aagtaaaagt tacgggaaaa aacaagcatt
    18601 aaaagacttg tctctctcga taagacaagg ggaaattttt ggatttttag gacataatgg
    18661 tgccggaaaa tcaactacga ttaaaagttt agttagtatt attgaaccat catcgggaac
    18721 gattttagta gatggaatga aattgacgga aaatcgattg agtatcaaac aaaaaatcgg
    18781 ttatgtgcct gattccccag atattttttt acaattgacg gctggagaat attgggactt
    18841 gatttcagct gcctatgaat tagacacaca aaaaaaagaa aagcgtttag cagaattgac
    18901 agcgcttttt gatatgtata gtcatcaaaa tgaaacaatc gcaagttttt cacatggaat
    18961 gcgtcaaaaa acgattttga tcggcacgtt gctgccagat ccagatattt gggtattgga
    19021 tgaacctttg caaggacttg atcctcaagc agcatttgat ttgaaagaaa tgatgaaggc
    19081 tcatgccgca aaaggaaaaa cagtcatttt ttctacacat gtattggata ccgcacaaca
    19141 gttatgcgat aaactggcta ttttgaagaa aggtgaattg atttatcagg gggcagttag
    19201 tgaattattg aatggctcac ctgatgaaac gctagaaaag atttatttga aaatggctgg
    19261 aaggcaagcg acaggagatg gaccgtatgt ataagcgaca attattagaa ttaattcgag
    19321 ttaatctttt atatgttaat ccacagctca cgaataaagc aagagagaaa ggaaaaaagg
    19381 gaaagcgatt aactatctat cttttgtctc aaaatctttt ttcagggctg atttttttac
    19441 tgatttacgg atttgctatg tttgctatga actttgttaa attgccaggt ttctttactt
    19501 attacgtggc actttttggt atgttaggtt taactcaaag tatatcagtc atttataata
    19561 tcttttttga aggaaatgac ctgcaagtat ttttgcctct gccttttgga caagaacagg
    19621 ttttttcagc aaagattttg gtagttgctt tgaccatcat tccttttgtt tttccgcttc
    19681 tagtcatgtt tattttaacc ggatggcgag ctgatctagg gattttgctt actatcatta
    19741 tctcaatcct tttgttttct ctatttttga ttattctttt ttgtctggct agtttgattg
    19801 tttttggttt ggctcgaaca gcagtgttta aaaaacatag aaaaacagtt tctagtatct
    19861 tgcttggcag ctcgatgctg atcgcatttg ctggaatcat gtggatgaat catcaaacgg
    19921 ggactattga aaatgagtta acagatcggc atccgatttc gatattattg ccattctatg
    19981 atgctgccag tcagccactt tctcaacaag gtttattggg ttggggaata ctagctatca
    20041 cggctctggg atttttcctt gctgtaaaac agctgatagt accaaagctt acagagcaac
    20101 tgactgattc aaacagtgag ttcaaaataa aaagaaccca taaaaagaac caaaatgtgc
    20161 atcagctttt atttagttac aatgctcaat tagtaaaaga acctaatttg attatgcaag
    20221 ttctttcttc ttccttgtta actccaatca tttttattct tgcttttgcc ctaggaggcg
    20281 aaataaaatt aactgattta gataataagt taatcggtgt tgttttcctg gtagggatcg
    20341 cattggcttt cttaacagtt aatcagactt cttttatcag caatcttatt tctttggatc
    20401 aagaaaattt tctgtttatc cgttctttgc caatctctat gaaccaatat ttaaaagaaa
    20461 agttcagatt cggatggata ttgcagagtt ttttaactgg tgggatcgca ttattaagcg
    20521 gactgtcttt tcagctgcct ttgtttttta tccctagctt gctatttggt tctttattcg
    20581 gctgctactt attgtcacta cgttatttcg ctagagatta tcggttgctg ctgctgaact
    20641 ggaccaatat taaccaatta tttaacagag gttcaggaag tttgggttta gtcgcaacta
    20701 tgatgggttc attgatcatc agtattattc ttatcgtctt atacggaatg ttggctttgt
    20761 tttatccatt ttggttagtg aatattcctg tgatcggttt agttctaata ctttccgctc
    20821 tatggatcgt ttattatcaa cgaacgtttt ggaaaaaatt tgaataaaga agaggggccg
    20881 tgacaaaaat catgtcgcag cccctttttt acagatagag ggtgttcaaa agtcagtttc
    20941 tcagtattaa gtcaatttta tgtaaaaatg tgagaagcta tttttacaaa acttttttta
    21001 atactcataa ttacttctta ttgctcctgc ggttacttgt cgcaatgttt gtgagatatt
    21061 ttgtcatcgc tcaaggctga acgtctcttt cacaaccttt tctttgttac acttcgttta
    21121 tttaaaaagc cgtttttttc gtttcatcat gattgcgtca cgctttactt tgaaattacg
    21181 gatgttatgg ttaaagactt ctgataatac aagacctaaa gcgattgctc cagcaatcat
    21241 aattgcttgg accgcataat gagccgcttc tcgataggat tcagtaacga gatttcgaac
    21301 cgcttgataa gctaaaccac cagggactag aggaaccatc ccgggaatat tgaaaatcgt
    21361 tacgggcatt ttcaaacgtt tagagaaaag atcgctgatg aatgctactc ctaaagaacc
    21421 aagcaatgaa ccaaaagcag cagatccgcc tatttgaaca cagatccagt agatcatcca
    21481 gccaaaagca ccagaaagac cacaacaaat caatgaacgc ctaggtacat ttgtaataac
    21541 ggcaaatgcc gcagtcgcta aaaaactaaa agaaaactga ataaatatcg tccccatact
    21601 tgtgttcctt tcctaataaa aaagctggaa aataaaagca atcgcaaaac cgatcatcgt
    21661 agcagtcatc atcgcttctg ttccgcgaga aactcctgag acataatgtc cagctagcaa
    21721 atcacggata gcgttcgtaa tttgaactcc aggaactaaa ggcatcacac atccgataat
    21781 gattagatcc tgattgcttc ctaagccaaa acgtacgctg tagatagcgg ctatgccaat
    21841 caataaagaa gataaaaatt ccgataaaaa tttgattcta aaaaatttca aactatatag
    21901 ataagctgcg tatcccaatc ctccaatgac taaagtcaca ggtaaatcgg tcatctttcc
    21961 gccaaataac accataattg ttccactgat cactgccgcg ctaacgattt gcaaccagac
    22021 aggaaagaaa gaacggtctc ggtcgattgc ttgcagttcg cagtaaagtt cttctaatgt
    22081 tagctctgaa gcaacatatt tacgtgaaag atcatttact tttactactt tttctaaatt
    22141 aatcgaacgg ttggtaattt gttccattcg gatggtcgag gtacgatcaa gaccaataaa
    22201 gagaccggtt tgagtgacat agctgaccaa acggaaattt ccagaagcta aagcaattcg
    22261 actcatcgta tcttctaccc gatacatctc cgcattgctt tccatcatga ttttcccagc
    22321 gagtaaacaa gtgtccagca acagatctaa ttcttttccc gtcatagcat cttctccaat
    22381 aattgattga ttagttttat tttactacaa aacataccag aagcgtaaag aataaaaaaa
    22441 cttgaggaag gaataaaaga ggcgtatact gtttttgtat tgcgggtgta gtttagtggt
    22501 aaaatcccag cttcccaagc tgatgtcgcg ggttcgattc ccgtcacccg cttaaaatag
    22561 gttgtgaaaa agctgctctg caacaagtaa taagaacagc caagcaaaaa caacttctta
    22621 tgtttttaac ttggatggac ttattaccgc agatgcaagc ttttgcgcac cgtttaccaa
    22681 gcggttgtga aatcagtgta ttgtcttgag tatgaagaaa atttttgaat aataataaat
    22741 accttacaga tagagtggtg actgattaga gacgaggaat ccgtctcttt tttgtatttt
    22801 taagccatct taaaacaggt tttatttttt gctgtttcta tttctttcta gtatttattt
    22861 ataggggaac atatgttaag atttagggag ctgttagtaa aggagtaaag aaatgagcaa
    22921 aaaaacaacc aaggcgatcg tggggatcgt cgctgctgtg attgcagggg cattcggtgt
    22981 ttccaccaca caaaaagatt cagatatctt tcagcagatt acaactattt ttgattccac
    23041 accacaagct tctcaagtag cggatgtgcc agaatatgat ggaaaacatc aagaaataga
    23101 aatcaatggg aatcagccgg aatttacaaa agaggatctt agcctttcca aaggaacatg
    23161 ggaaagctat tcaaatattg ataaattgaa cagagtagga caagccaatg ctatgcttgg
    23221 aaaagatttg ttccctactg aaaagagaga gcctttgtat attgacccga caggttggaa
    23281 acaaaagaaa ttaagtgacg ggcaatggtt atataatcgc agccatttaa ttggtttcca
    23341 gctgactggt caaaataaca acattaaaaa cttgatgaca gggacacgtt cgttgaatgc
    23401 tccatacatg cttgctcacg aaaatgatat tgcggcatat atcaaagaaa caaatcatca
    23461 tgtacgctac cgtgtcactc ctcattttga ggggaatgaa ctggtagcca ggggagtgca
    23521 attggaagca gaaagcattg aagacaaaaa aattgaattc aatgtcttta tctacaatgt
    23581 tcaagatggc tacacgatca attatgagac aggacaagca acaaaaacaa aaaattagta
    23641 gcagctattc gaatttttta tgaatattat gaaaaaattg tgtaaaaaca tacattcaaa
    23701 gaaatatagc gtatacttta tgaaaagagt acttttgata aagctacaga aaaaaggaaa
    23761 aaaacataca aatagtgttt cttaatttat tcggaagttt ggaatatttg tttgtagttt
    23821 tccctttctt cgagtaaaat aaggttgtag aaggtaagct ggaattacct tgcgggaaag
    23881 aggtgtagaa tcatgggttt tacagatgaa accgtacgtt ttggttttga cgatagtcgc
    23941 aaaaaagaag tgagcgaaac attagcgatt gtttatcggg ctttggaaga aaaaggctac
    24001 aatccaatca atcagattgt cggctacttg ctttctggag acccgtccta cattcctcgt
    24061 tatcgtgatg ctcgaaattt gattcgccgt cacgaacgcg atgagatcat ggaagtaatt
    24121 gtcaaggact acctctcaaa tcatggagtg aatttatgag agtaatgggc ttggatgtcg
    24181 gttctaagac agtaggtgtt gctatcagtg acccctttgg ctggacagct caaggagtag
    24241 agatcattcg gatcaatgaa gcagaagaag agtttggctt tgatcggttg aaggaattag
    24301 tagcacaata tgaagtagac cggtttgtag ttggattacc taaaaatatg aacaattcca
    24361 tcggaccaag ggcagaagct tccatgtcat acggagaaaa gatcaaagaa ctattctcct
    24421 tgccagttga ttatcaagat gaacgattaa ccactgttca ggcagaacgt atgctagtag
    24481 agcaggcaaa tacttcaaga gccaaacgaa aaaaagtcat cgataaatta gcagctgtga
    24541 tgatcttaca aaattattta gacggctcag gtaagcaatt attttaataa taaaagaaaa
    24601 gaggtaatct tatgacagaa cataaccatt cccacgacca tgaaggacac gaacacatca
    24661 cattggtgga tgaccaagga aatgaaacat tatatgaaat cctactaaca gttgacggac
    24721 aagaagaatt tggacgtaac tatgttcttt tatatcctgc tggtgtttca gaagatgaag
    24781 atgtagaatt acaagcttat gcatacatcg aaaacgaaga tggaacagaa ggcgagttag
    24841 aacaaatcga aacagacgca gaatgggaca tgattgaaga agtattcaat acattcatgg
    24901 cggaagaaga agaatagact ataaagctag gagaaacctt gatatgatag ggtttctcct
    24961 ttttttattt tttactgtat gccatattgt atgccatcta gtcaatattt aagtgttgta
    25021 ctaatttttc aacagtcttt tctttcgttt cttttgccaa gtgaccgtaa caatcgatcg
    25081 tctgactaat ttttgtatgc cgtaatctag tttgaacttc taccatagta gctccagcgt
    25141 ccaagagcat tgaagcatga gtgtgtcttg ctacatgcac accgagaaat ggaaccatag
    25201 ctttttcaca aatagtaatc aatcttgcat aaatagcgtg gtgtgtgatt agttctttat
    25261 agataccaca aaaaatcata gtcggatcag ttacaccaag tgtcagcata tatttccgct
    25321 gattaatctg ccattttttt aatatagaaa ttgttctttc atctagtgga agaaccccag
    25381 cactacaatc agttttagga tcactgattt tatagccatt tttggattgg cttagtgttt
    25441 tattgatggt gaccgtattt tcttgaaaat caatgtctga ccaattcaat gcaagggctt
    25501 cactgatcct aaccccgcta taaaagagaa agcgtagtaa cgttttatca aagtcgtttc
    25561 gataagtttc aggtttgctg tttaggtaat caaacaaggt gatcagttgt tctttactgt
    25621 aaactttgac cttttcttta tgcttagtgg tggttctttt aggcattaat acgttgtcta
    25681 gtggattagt ctcaataatc ccaagattga tagcgtagct aaaaatacgc ttagaagttg
    25741 aatgaagtac cttgtattgg tcagtagttt cagcccagct atttacaatt tgttgggctt
    25801 tttttaccgt gacttcattc acacgaagat ttccatattt tggaagaatc cactttctaa
    25861 acttgatttc agttttcatg aaggtggctt ctcggacagt ggttttatat gtttctagcc
    25921 acaagtcata aacttcttta aacgtttcgt tgttcgcttt tttaataccg tttttatcgt
    25981 agtccacttg caagcgtgac aaggcaagct gggcttcttt ttttgttttg taacctcgtc
    26041 tacgaacctt atcaggtttg ccagtcattt tgttcattcc aagataggct tcatagagcc
    26101 aaaatttacc ttttttagtt tcatattgct tgaatgttgc cataatagtt ttccctccat
    26161 agtgtagcaa gccgtggggg cggttggagg aggcagttta ttattatttt gaatagtgtt
    26221 tatttagctc agtaacaaga gctgtgaact cttttagaag atcattttca taaaattctt
    26281 gatactcttt tttctcatct gtattttttt ctgaatcatt aattaatgag tcatatcgta
    26341 aaagcgtatt tagaagagct gcgagataag caattggggt actcccgtca ggaaattcaa
    26401 ttcgagatcc tgttttaaga tattttattg cgtttgttaa ataaatcttc tgtgagacag
    26461 tcaggttatc ttcttcgatg ttattgatag cattagaaag attagaatca attatagagt
    26521 tatttttttc aatagttttt ttcaattgct tttcaagcac atttcgctta atttcgggca
    26581 tcatatgcat atctcttaat gtataagatc cgtttaatag ataggtagtg gatacatcta
    26641 atacctttga aagttgttta agcctggtgt tattgggaag attatagcca ttttcccaat
    26701 tactaacggc acctttactg gccggtgggg ataatttacc tccaagttgt tccattgaga
    26761 gtcccttttc aattctagcc agtctaattc tttgtcctgt ctccactgct tcggggtatt
    26821 tttttctagc cacattttca ccaccattca tatattttct ctactatatc acgatttaaa
    26881 tatgtgtgaa acataaaagt tcaaaaaagt atgttaaaat tcttgcaaat gaaaaggtat
    26941 gatggtatat tgagtttata agaggtatag aaatgtattc aagagcctct tgcatttttt
    27001 taaaatcttt gtatagaaat gtatgctaac gaggggggat aaatatggaa ttaaaaattg
    27061 acaaactcac gcttgatgat ccagctttta aaattattga cgatcgagtt gttcaacaag
    27121 tagagcaaac tcaaggaacg ttactttcat tattagaaaa aaagtaccag tttccaaaat
    27181 atatgaacaa ggcacaagct gctaagtata tgaacgtttc atacaatact atgatgcaga
    27241 aatatgttcc taacggttta aagttgatta tagttgatgg agtcgtaaga atttcacaag
    27301 atgaatgtga tcgctttatg gaagaatacc aaaaataaac gcaagccgtg ggggcaaagg
    27361 gattaatagg aggaaataat aatggaatcg acacaaaata aagcaatcga aaaagtatta
    27421 tcaacaattc ttacagaaga cacagcggca gaattagcaa acttggaagg caaagcgcta
    27481 gaagaaacgt ttgaatgttt gtacgaacaa atggactatc aaaaattgct accacaagaa
    27541 ccaacggtaa gcggagtatt gcgtggcttg aacgatttag tacaagcaga gtttaaagaa
    27601 cgactttcta ttgaagagta tcaagaaatt ttgtatcagc aagttgagtt gttagcgagc
    27661 ttgctagggc ttgaactgga ggacgttgaa tgaaaactga aatttggaac ggacacatta
    27721 taagatttgt cgatattaat gatgaatggt gggcagtggc gaaagatgtt gcggaagcgt
    27781 taggtcttaa acaggttact agagccattc actctttgcc taaagatggg gttactacta
    27841 gtaaggtcat cgattcattg ggcagaacac aagatgtaaa catcatcaac gaaaaaaaat
    27901 atttaccgca tggcattcaa aagccgtaaa aaagaagccg aggcatttca agactggatt
    27961 ttcgacatca tcaaagaact acgccagtcc acaggacttg aaggctttca agtattccgc
    28021 atgctagaca aagaacacca aaaagaagca atgactaaat taagccacgc tattactgaa
    28081 ccaaaaccag tagactacat taaagcgaat gtgatagcca ataaagctgt atcaactatt
    28141 tatggtcatt ccaaaatggt aaaaaagaaa gatatgactc ctgaaatgtt ggttgatcgt
    28201 gaacctattt tagatgagac agtagaatta atgactgtta aagaaaaata tgggttgcag
    28261 ttttcagtga gtgagaaaat ttataatcgt tctgctgaat tgcagacaac ttaggagcat
    28321 taacatgatc gagaacaaag aaaaagcctt atccataaca gtcttggcgg acaggataaa
    28381 ggcaacttaa ttgggataaa acccgatatt ctttgttccg attataccat attaaaaaat
    28441 cggagggaac cattatgaaa cgaaacttaa ttataggaag cattttaatc ggcggaattt
    28501 tcggcttatt accattaatt gcaaaaatag ctatctttgc aggattagcg ttatatctct
    28561 tcactgctta tgacgagtac gactatcaat taagaactgg agggcataag tagatgtaca
    28621 aaacaaaact attaaaccaa ctcgatagcc ttgaattaga ggaaattaat caagggatcg
    28681 ctgaactaga aaataatatc gggaaaacct actttggaaa ttcattcaat gaaaaactaa
    28741 cagttttgta tgtactcaaa aaacatgcag aacacaaaat aatttgtcga gaaatcaacg
    28801 agctaaaaaa tcagatttta accgcatggt taaacattac tgatatgcaa gaagcaaggg
    28861 tgaaaacatt taatacatgg gtaaagtatc agaatcaatt aaaaggtgct gaatttgttc
    28921 gggatgggtt gaaatacgaa cttgagcagt tgaagctcat ggaggtgtct gaatgattta
    28981 taccagtatt ttgaaaaagg aggagaggtt tattggcaaa taattatcaa gcagcaacgg
    29041 aattacaaaa gtcgggatgg tcaatttatc ccttatctcc tggaactaaa attccaataa
    29101 aaggctctag tggttggaat gatgcaacta acgattctaa tcaaattaat gaatggtgga
    29161 cggaagatgc aactagaaat attgggttaa tgcttgaaga tcatcaaatg gtggtagttg
    29221 acctagatca acattctgaa acacgaaacg gcgttgagaa ttataaaaaa cttgcttcaa
    29281 tgtatgatcc atttccttct acttatacgg aaatcacacc aggtaaagga atccatttct
    29341 tctttaaaat tcctgatggt gtaattatcc aacaagaaac aggtgcattt gctgatatct
    29401 tcgggcgtga tgataaaggt aaaagtttaa gcgggattga tattatcact aaaggagttc
    29461 caatagcgcc aactatgatt atttcaagaa gtgttgggag agcatatgaa cctattgaaa
    29521 acgtatctct aaataatatt gcaccagctc ccgattggct agtgaaccta ctgctaaaaa
    29581 aaccgcaaga aataaagact caataccgac ccgtagcaaa aacagggaca gcaaaaaaat
    29641 tagatatgat tgttcaaggc gctggcgaag gtagtagaaa cgatcattta actaagttat
    29701 gcggttggtt actatggcac ggtgtagata atcaaacttt agcagaattg atttatacag
    29761 caaatgcgta caatgaacca ccgctaaaag ataaagaagt gaatcaaatt attcgatcga
    29821 tgattaaaaa agatttgagg gggaaagctc atggctcata agcataaatt agatagtatt
    29881 ttagactttc ccgaagccag cgagcgtgaa gacaatatca ttgaattgaa aacctggatg
    29941 tcacgattaa gatgcaataa agacgatcag attaaaagta acagtgttgt gaatgcagaa
    30001 ttgattttaa ccaatgatag caatttagct ggaacaattg cttataacga atttagcgga
    30061 tacattcacc tattaaaaga ttctccttgg atcaatcgta ccgctggaga atgggaagac
    30121 agttttgagg atgccttaac ggcatatatt gaagaaaact acaatgtagt ttttgatgac
    30181 aacaaaatac acaaagcagt agtaaatgtg gctagaaaga atgttttcaa cccagtaaaa
    30241 gaacgtatcg aaaaagttaa atgggatcaa cagcctagac tggaaacact gtttattgac
    30301 cttttagggg tagacgacaa cctttacaca cgagaagtaa caaaacgttg gatagtcgga
    30361 tcagtcgcac gtatctataa accaggaata aagtttgaga tcgttccagt tttggatgga
    30421 ccgcaaggga ttggtaagag taccgttcca gcattacttt acacggatga ttttttcacc
    30481 gattcattag attcgctagg tgaaaagaaa gatgattata tgcagcttca aggcaatgta
    30541 attattgaac ttggagaatt atcttcaatg aacaaaacta aaattgaaca gataaaaaac
    30601 ttcatcagtg ccaagatcga taaaattaga cctccttatg gtcggaatgt catagcttgg
    30661 gcaaggcaat gtgtatttat tggaacgtcg aatgatgggc agtatttgaa agatgataca
    30721 ggaaaccgcc gtttttatcc tttgccttgt aagaataagc caaagatgaa tccgtttaaa
    30781 acaaaggatg aatattttct tcaagtgtta gctgaagcaa aggtgctttt tgataaagga
    30841 caacgaatct attttagtcc cgatgaagat aaagaagtct tagaaattgc aaaagactat
    30901 caagaggatg ccaagatgga gaatccaatt aaagaagcaa tcactaaata tttggaaatg
    30961 gaaatcccgt ataaatggga agaagctcca gcgtgggcga gacgttctta ttatcagaac
    31021 tatcctgaca gtaaatgtaa tgagaaaaat ttacagcatt tctataatgg agctactatg
    31081 caacaagagt tttatttagt tgatagcgta ctcaccgctg atattttgga agctgttttt
    31141 gataaacaag caaaggattt attgaatggt cgatcagatg cagaaactaa aaagattgcc
    31201 ttgattatta gcagtattcc tggttgggaa cggaagcaac tgccaaaaag aaataaacga
    31261 cgaggatttt acaataagtc aaatgcctat gaaaataaaa atcgagctgg aaagtacaaa
    31321 aagtaacggg tagtaacggg tcagtaacac ctagcccgtt actagtttac ccttactgcc
    31381 ccaagggata cagcaatttg gtaacggggt aacaccttat ttccttaaaa cttttgaaat
    31441 agttagctaa tatgctgggg aataatgtta ctggatttta cccgttacct gttaccaaaa
    31501 cgctagaacc cttgtccccg ttgggattga gtggtaacgg gtacctgtta cgtaggtgtt
    31561 accacgtgtt acgaatcaag gaggaaagta aatgctcaat attatttcaa caaacaaagc
    31621 accgaacttt caatatacag atgaaatgga tcggtttcta atgaacacgc tggcatttag
    31681 tgtgggattg gtaacagaag attattccac atttgatcca gaagtattaa agataatgga
    31741 agaagaacct gattggctgc aggagtcagt agcgtggtgt caatcgctag tagtgggatc
    31801 attggtagat agtggcaact atgatgatac aggtgagcta atggatgaat ttaattgtct
    31861 gcttaaccta tatgatcgag cgagacaacg agagcttaca tcgaatgagg ataatttgtt
    31921 tttaaacatc catgataagt tcttggcttt gctactaaca gatgatgaat tgataactaa
    31981 tttattggag gtagaataat tatgcatatg gaaagtatta gatacttcga tggtgagaaa
    32041 cacattgagt attcaaacac tcaagaagat gtagacttta ttagttttga ggaagataaa
    32101 atcactaaag ttaattttaa agacggaacg tgcttgaaga tagtttcgcc ttatattgaa
    32161 tacaaatcaa aatgggaaca agatgattct gacgaacgag atcgctttgg ttggtaatct
    32221 atggcagtta aaaaacaatg taatcatgct ggatgtaaaa cattgattga ttataggcag
    32281 aagtattgtg ggaagcataa agcaaagcaa acggcaatta agcgtgaaga aagaaagcaa
    32341 agcgaaggta aatattttca attctatcaa agtagaacat ggcgaaaggc ttcatacttg
    32401 tatcggttaa atcatccagt atgtgaggat tgcctagaag aaggcttgat aaggaaagct
    32461 gatgttgtag atcacaaaat tgaactaaaa gatgattgga atagacggtt agatgaaact
    32521 aattttcgtt ctttatgcca tgcacaccat aattccaaga cagccaatga aagacaaaga
    32581 cgagaaaaga gtaccctttg agttaagggg gcatagtgtg aaacttactg acaatcgatg
    32641 cctactcatt ttggtacaaa taaccgttgt gaaaaggcat aagggtaatt acaaataacg
    32701 attacacttg taatcacaag tgaaaaagta tataatgaaa gtaggagatt tattgaaaaa
    32761 tacaattgtt attactgata aaacagggaa acagcgcacc attgagttac ctcattttgg
    32821 tcaagtgacc attcaaatgc agaacggaaa aatcatttat atagataaat tagataaaga
    32881 aaaattctga tcgaaaaacg aaggaatgtg agcttaattg cttacgttcc tttttctttt
    32941 gtctgaaagg aggacaacat gggacaccct aaaatattac acgatacaaa aggcaatata
    33001 tcaagtgaag aaaaagcaat ccgtgtggac gctagagaag aattgttcaa gcagcaacca
    33061 ttaataaata tcacgccccc tgactggatg gcagcgagcg ctagaagtga atggaatcgt
    33121 atcgtaccaa cattaaaaaa agattatcca ttgagtgaag cagattatgg ttcattggta
    33181 gcctattgtt tagcctttgc tcgaatgaaa acagccgaag ccgagataag aaaatcaggt
    33241 acgtttatca catgcgaaaa cggagtaaag aaagccaatc cagcggttcg ggttcaatca
    33301 caagctatga gtgatttgaa aaaacaagcc acctcactag gtatgacctt agaatcacga
    33361 tcaaaactag ctttgaacaa ggttaaaaat gatgaacccg aagacccgtt caaagagttg
    33421 atgaattcat gaatgattac attgagaaag tcctatcagg taagctgatt gcacccaaga
    33481 aaattatcca agcgtgtgag cgtcatataa gcgatttgga gcgttctaag tcagatagct
    33541 tcccttatgt gttcgatgaa gaacaagcca ccaaagcgat taaattcatt gagttgctac
    33601 catctacgga cggtaaagca atcaagatgt taggatttca aaaattcatt ctaggcagtc
    33661 tttatggctg gcgtactaaa gaagggaatt acaggcgatt caatcgagcg tttaccagta
    33721 tgagccgtaa gaatgggaaa acgtatatcg caagtggcat ggctgccaat gcgttgatta
    33781 tggaacaaga accagcagaa gcaaggcaag tattgtttgt aagtaacgct ttgaaacaag
    33841 ctaaattggg ctatgatatg ctgtctaatt cacttagaaa cgtggtcaag tctagtaagt
    33901 ttttaagacc acaactgaaa attatgaact ctaagattca gcacttgccc tctaattcgt
    33961 tcgctatggc actggctagt gaaaccagca cgctagatgg gtttgcacca acaaccgcaa
    34021 ttcttgacga gtggcacgaa gcaaaaactc gtaaaacgta caacgtcatt aagtcaggaa
    34081 tgacccaaca aaagaacggc ttattgtgtg ttattagtac cgctgggctt gatttaaacg
    34141 ttcctatgta cgaggaatac ttattgttag atcgtgtgct aaaaggcgaa gaacaagccg
    34201 acaggtactt tatagcgata tgggaattgg acgatcccga agaaattcac gatcaagaga
    34261 aatggatcaa agccaatccg atttttgaaa gtgaagaaat caaaaaagta atgattccaa
    34321 ccattcaaga tgatgtgaac cttgctttga aacaaaataa ccttaattct gtattggtga
    34381 aaaacttcaa tttatggaga caagcgagtg aggacagcta tatgattgct gaagactggc
    34441 aggcaaccga agtagaacca caagatatta caggcaagcc cgtttatatc ggagtggatt
    34501 tatctaaaac agatgattta actagcgttt catggatcgt accgctagat aacggcgaac
    34561 tttattgtga ttcacatagc tttgtagcca ccaaatatgg gcttcaagac aaagaaaagc
    34621 gcgacggttt gccttatcga gaactagaaa aagctggtga gtgttccatt actcaattag
    34681 aaagtggaat cgtggactat gaccaagtat ttcagtttat tcaagatttg attcaagaaa
    34741 atgatttgga atgtatgggg atttgttatg acccgtataa cgctaattcg cttatcagta
    34801 aagctgaaaa agccaactac ccaatgttag aagtaagaca aggaacgatt actctaaacg
    34861 ttccgacccg aacttttaga gaacaagttt atgaaggcaa cgttattcac aataaaaata
    34921 cgattctcac ccatgcagtg aacaacgcta ttttaaaaac ggataacaac ggcattcaga
    34981 ttaataaatc aaagaacagt aacaaaattg atccaatagc tgcattaatc aatgcctatg
    35041 tgtttgcaat ggattacttc accacaacgg aaggagcgaa agcagacaat gaattttata
    35101 caagtgaaga attttctttc taattacatt cataccgttc ttttacttct tggattggtg
    35161 tgtgtgttgg ttgctatcac cttactaaca aatgtctact atggcttgtt agcgctgggc
    35221 atagtgctta ttggggtagc ggtcatgcta aatacagaac agaagggagg ttaaaagatg
    35281 gcatttttta aagcgagaca aaatacaacg ggagatcctt tcctacaaca tgtggtatca
    35341 atccaatcgg atgattacac caccagcttt acaagcgttc gtgcattaag aaatagtgat
    35401 gtgtttgcag ccgttcgaat tattgctagt gatattgctt caagtccgat tcaattggtc
    35461 aaaaacaata tgccacaagc tgataatgaa ctggtgaagt tactaaacga gaaacctaat
    35521 tcagaaatgg atggttggca tttaaagttt gctttggcgg tcaatatgtt gctaaacggt
    35581 aatagctttg cagagattaa acgcaacggt gaaaaggtag aagaaattca cttattacct
    35641 aactcaagtg taacggttac tcaattagat aatggtacgt tgtcttatca gattggtgat
    35701 aaaaaaagac gtgtgaagtc tagcgatatt ttgcacttca aatatttcac ccaagatggt
    35761 ttaacaggat taccaccact ttatgcgtta cgtgatgaac tgaaaattca acaagctggc
    35821 aaccgcacat tgcacaattt ctttactcgt ggtgtcagtg ggtcaggtat tttgaaggtt
    35881 cataagtcag atttagacgg atcagcaaag aacgcaattc gtgaaaagtt tgaagaagcc
    35941 aacggttcaa gtagtggaga taatgctctt agaacgatca ttcttgatga aacaatggac
    36001 tacaaaacat tagaaataaa caccgatgtt ttgaagctta tcaattccaa tgattggaac
    36061 acaaaacaaa ttgccaaggc gtttggtgta ccgattgagc gtttaggcgt tgaaaatgaa
    36121 cattctagca ccgttcaaag taatctccag tatatccaaa gtacgctgat tcattacttt
    36181 aatgtgtttg tgagtgaatt agatacgaaa cttgaaacca atatccgctt taattccgat
    36241 cagttactag agactgatcc tgaaacaaaa gtaaaaaatg tattggatca ggtcaaaggg
    36301 tcacttctca cgattaatga ggggcggtcg aaaatggggc taccccccat ggatggtggc
    36361 gatcgtttac tagcaagttt gaactttacg tatttagata cgttggagaa atatcaatta
    36421 aaagaacagg aaggagttac accagttgaa taatgaagaa gaaaaggaaa aacggctgac
    36481 agaagaagct gagctaacag ccgattctcc caaagtagag aaagaaaatg aaaaacaacc
    36541 aacagacggc aaaactattt caggctatgc gttgaaattc gggcaaccgt caaaagattt
    36601 aggcggcttt gtagaagtga ttacacccga agcattaaaa gaggtggatt tatcaaatgt
    36661 gttcttatta tatggacacg attattcaaa gccactagct agtgtcaaag caggtacgtt
    36721 gaaattgaat gtcgatgata caggattaca ttttgaagca gagctaaccg acacgaccta
    36781 ttcaaatgat gtgtatgaga atatctcaaa aggggttgtt gattctatgt cctttgggtt
    36841 cgtgcttgga ttagattcat tcgacaaaaa agaagatggc acaattgaac gatcaataga
    36901 taaaatcaaa gcacttaatg aaattagtgt cgtgaccgtt cccgcttatg attcatcaaa
    36961 tgtccaagta aataagcgtt cttacgaatc gtttatgagt aacaaccaag caaaacaaac
    37021 aaacaatagc ttagaatcca cttctaaagc acaaaaggag agtaataaca tggaaaaaac
    37081 tttaatcgat aacgaaaaaa cagaattgcg tgggtatgaa gaatatatcc gttcacaagg
    37141 cgaagtgcgt gatggagtca ctactgtaaa tgcagcggca gttgttcccg aagaagtaat
    37201 cggtgaagtc tttgatttga aacgttcaaa ttataactta gctcaatatg caacagtaaa
    37261 aacagtatca aatggacaag gtaaatatcc agtagcaact aaccaacaag cagtgttagc
    37321 aacaaaagct gaacttgctg aaattggtga tattgacgct gaaatgttta cttcagttga
    37381 ttataaagta gaaactcgtg ctggtaagat tgccttatca aatgaggttg tggaagattc
    37441 agcagtgaat attgtacaag aggtcaaaga tcagttagcg aaattggtag aaaacactga
    37501 caataagcat atcatggatt tattaaaaac attcactaag aaaacggctg ctacgttgga
    37561 cgatttgaaa caactataca atgtggcatt agacccagca ttaaataaaa tggtaattct
    37621 aaaccaaagc ggatattacc acctagatac attgaaagat tcagatggac gttacatttt
    37681 acaacccgat gtgacagcac ctagtggtaa atcattattc ggtatgccag tagtattgat
    37741 tgcagataca ttgtttgcca atcctaaagc tggaacattc cctatgatta tgggggatat
    37801 tgcacaatct atctttgttg ctcgtagaaa tcaagtaacg actcaatggg aaaaattcga
    37861 ttactactca caaggacttg cagtgatcgt tcgcaacgat tataagaaaa ttgatcaaaa
    37921 tgcttcagtg tatattgagt ttacgccagt tgtaacacca aaagtataga aaaatattgg
    37981 gcggcggttt atcccactgc ctttttttca ttaggagaga taaaatggta attttggata
    38041 gcataaaaaa aagtatgcgg atcgatcaca ctattgatga tgactttatt caacaattga
    38101 ttgatacagc aggcgaatat ataaaaagtg ctattgatag tagcgcaacg gataaagata
    38161 tggataatta tcagcaattt gatttggcgg tgtcattact tactcaacat tggtatttga
    38221 accgtcaaga agccagcagc gaacggatac cagtaacggt acaagcatta gtacaacaaa
    38281 tgagaggtgc ttattatgcc aatcattaag aatgtaaatg aattgactga gagagtcaag
    38341 tttaaaaaga caaaacgggt aaaagatgaa gatgggcaga tggtggatag tgaagaaact
    38401 gtatttgaat gttgggctaa tgtgcgttca caaatgctaa aagacgtgct tgctagtgta
    38461 ggtaccattc ttgaaggaac gttgacgttt attattcgat acgatcaaga ttatgaacta
    38521 acaaacgata tgaaagtagc ttggaaaaac aagagttata aaattatttc aatcaatgaa
    38581 ggaacggcgt ttaaagatta tacaacgatc atagctaaac agatttctta gaatgattac
    38641 acttgtaaac gttgtgggta tttagtataa ttaaggtagt aaatgaaggg ttagctgcct
    38701 gaattttgca agacttagct agtcgaaatc tattgttgga ctgaaaattg taggtgtggt
    38761 tgcaaacatg ctggactaag aaagatgata gatgatgaac gttcaatttt aatacaggca
    38821 tgctaggact gattggatgg ttttggcaag ctgattgtgt agaaatcaaa gaacacgtct
    38881 gttgtggacg tgtttttttt gttataattc aaaagaggtg atttaatgga caaagcagag
    38941 tttacaaatt tttattggaa gtattatcta catttagaaa atgagtttat taataccaca
    39001 gattttgtga tgctcgatga gaataatttc aaaacattct ctattgaata tcaaaaacta
    39061 ctactagcga ttggttcgga gtgtgagatt attttcaaag aactttgcgg atttgatagc
    39121 agtagcaata aaagcataac tgattttaaa gctgttatta aatctagcga attaatgtgt
    39181 ttggataatg gtgttagggt tatgaattct ttaacattga cttcactaaa accttttgga
    39241 gatgggtggc ctgaagatac accagattgg tggaaaatat ataacagagt aaaacatggt
    39301 cgttcttcaa attataagga agcaaatttg gaaaatgtgt tgtatgcttt agcaagtcta
    39361 tatttattag aggaatattt gcataagaaa gtaatactag aaggtgaagt agactttata
    39421 agtccagagt caaatctatt tacgttatct tgggaaagaa aacattcgtc gatgcaaaaa
    39481 gtgacgtttg aaaaagtgga ttcaaattat gtagctccac tcattgattt taaagaacaa
    39541 gaagaaattt aaaaaccccc acggcttatt gaagtttttg tatgccatat tgtatgccat
    39601 ttggtttaaa tcgcttgaaa aatagtgata gataactcta attcatttag tcataaacgc
    39661 tgttttgata acctttatag ttctttgata tgggtttctc attcatggcg gaagaagaag
    39721 aatagaacaa aagaatgaat taataagata taaaaaaagg gattgtgaca aacgtcttgt
    39781 cacaatccct ttttccaaat aaacgatgtt caatcagctg cccctcggca ttcgctcgaa
    39841 aaacggcgaa atatgaagaa ctattttgcc atttgtttct ctaataatca ggtcaaaacg
    39901 ttgacttcac agtctcttcc ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca
    39961 caatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctga
    40021 acggcaggct cacgacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    40081 gttcaaccaa acgaaaatta tgaggagcta tttttgtttg gctgttctta ttacttgatg
    40141 caggacagct ttttcacaac ctcttttcgt ttagcactaa atttccttaa aaagtagaaa
    40201 agctttcgaa gcctcgtttg tttcttttac tgtataataa aagaaaagag taacaatcgc
    40261 taaaagatat aaacaagaga aaggaaatac aagatgattg gtctgattta ttttgtggtg
    40321 atcgttctgg cgaacacagt tggcgctatt tccgggatgg gtggaggcgt gttgatcaaa
    40381 ccgattcttg atcttatcgg cgcccattct gttgcaggga tttcttttta ctcaacagtt
    40441 gcggtattca cgatgtccat tgtttcgact gtgcgacaag tatcaagcgg gaaatcactg
    40501 aattggcaga ttgtcggttg ggtatctagt ggagctgttg ttggaggagt agtcggcaac
    40561 attgtattcg aagttttttt acagttattt gaaaacgaga agcatgtgca gctgatccaa
    40621 atttttttga cggtattgac cttggttttt gcgttcttct acaccaagca tcatcagcca
    40681 aaatttcatt tgacgtcatg gacttggtac cttatatgtg ggggggttct gggcttttta
    40741 gctagttttt taggtattgg tggtggacca gtcaatgttt ctttgttgat gctgatgttt
    40801 gcattgccaa taaaagaagc aacactatat tctctatcta cgattttctt ttctcaactg
    40861 gcaaaattgg taacgattgc tttaacaagt agctttatgc gctttgattt atctatgttg
    40921 ttttatgtta tccccgctgc tgttattgga ggtttgtggg gagcaagatt tagcagggtt
    40981 ttgtctccaa aaaaagtgac ctttattttt caagtaattg tcctcgtggt tcttttggtc
    41041 aattgttaca atgcctttgt catcttacaa acattaggag aatctagact aacgcaagtc
    41101 aaaatcactg aaacatattg ttctaaatga aaaaaggagt atactgtgat aaaaaaattc
    41161 atttaaaaaa ataaaaattg gctggtgatt tggatgttgc gggaaatgcg tttaggagat
    41221 gcagaagcac tacgagaaat caatgcggaa caattaggat atgatgtacc attaaccgtt
    41281 acggtgagac aaatgaagaa actgttgaag gagccagaaa aaaatttttt cttggtctat
    41341 gaagatccta cttctaaaaa agcagctggc tatgtacaag cggagtccta tgaatcgatt
    41401 ttcagcgatc cgatgttcaa tattatggca cttgctgtgt ctaaacatgc tgaaaaaaga
    41461 ggaatcggaa aggccttgat gactggcgta gaagaagcga aaagacgagg aatatcagca
    41521 ataagactta attctgcaga atacagagta gaagcacacc gattttatga acgcatcggt
    41581 tatcattccg ataaaatgca aaaaagattt ttgaaaatcc tgtaaatttt tatcaaaaaa
    41641 tacctggtca tcaagccgat acttgatgat caggtatttt ttttgactaa gaaagtaatt
    41701 gcatcaagaa acctgagatg atcaaaacga tggcaccacc aagccgattg cccatttgag
    41761 caaaagcaat aagttccatc cgattagctg cagataagac agctacattt cctgtaccac
    41821 ccatcgagtt gttgcataag ccagcagtaa tagttgattc gattggatat aaaccaaaca
    41881 aacgaccgac aaatcctgaa actactgaaa tcgcgataac acttgtcaaa cataagacaa
    41941 caaattgcca tgtcaaagca gaagccaata catttagatt caataacgca ataccgatac
    42001 cggcaagaac agctgctgtc aagtttttga caatcaagtt gttgaacatg atagctgcat
    42061 cttcgtaata ttctggtaat aggtttgcga ttttacagat aacaacgata atgatcatga
    42121 atgcgtaagc atggacttta ggaacaaaat agttacagat ttgtcctaag atgaagaaag
    42181 tcaatgatac catcaaaccg acacctaatt gtggaatgga aggttttaca tttctaggtt
    42241 ttccatcgcc aaggtcgcct ttttccatta atttaccatt accgttgaat tttggcattg
    42301 attgtcccgc acgagcaact aacgctgcac caatgatagc taggacattt gtcattgctg
    42361 tcgcaggaat caggcgggaa atgatagaag aagcgtccgt acctaaaaca tttgcataaa
    42421 tcgttgataa aggtacagct ccggctccaa caccaccagc cattgcgggc aatgagatat
    42481 ataaaacgga atttgcaaaa ccatttccga taagcatacc tacgagtcct acggcaaaaa
    42541 aggagacgac cattgaaata agtgcaacgg gaataaaacg aacagaagct ttcatcaata
    42601 gattacggtt cataccaagg atgctaccag taatcaaggc agcaatataa aagtctaaaa
    42661 agcccatatt gctgacaaag ttttctgtgg attttactac atactcaggt aagatcccaa
    42721 atgtagcaat cgcggcagag gcaaagatcg caaaaacgga gccaccacct aaataacttt
    42781 tgacgatagg gatttttgtt ccgataaaat gaaataaatt accgaaaatg accaaaacag
    42841 cgattgggcc aatcatattc gtcggtaatt ttcctaaagc cattgcaatg atcaggacaa
    42901 ggaccatgat caaataaagg ggcaaactta cgccggtaat ttttgttgac caaaaccctt
    42961 cttttttctt ttcctcaact gcagttgcgg ttgttgtttt ttgtgccatt tttacagatc
    43021 ctttcttatt ctgtatcgta tgaaaaaagc caaagagaag cggcaaaaga gcagtagttg
    43081 gctggctgct gttttgccgc agtcgctttt ttctttttag acgtcataca aagtttctgt
    43141 atgttctttt actataacaa tcttaatctt ctaaagattt atattcagga acccatttag
    43201 cagattcaac agcttctttg atatccgtga tttcttcacg attcaatttt tgtgcaacaa
    43261 ctgattttgc gacagtttca gcaatcgttt gagagaattc agtgattttt gctactggag
    43321 gcaagatagc tgctcctggt tttgttacat caacgattcc gcctaatgct cgactagctt
    43381 gagaaatgat ttcagcattt actcgtgtag ctgttgaagc aatcaaacca agccctaaac
    43441 ctgggtacat caacgcatta tttgcttgac cgatttggta agttacaccg ttatattcca
    43501 catcagcagc agggatacct gttccaacta aagcttttcc gtccgtccat tcaatcaaat
    43561 cttttgcttt tgcttctgcc aattttgttg gattagacaa aggcatgatg attggacgtg
    43621 gggtatgagc cgccatttct ttcacgattt cttcagtgaa tgctccaggt tgggtagaag
    43681 taccgatcat gatcgttgga tggattgctt ttacaactgc ttctaagttt gtcaattcat
    43741 cagcattagt gaattctgag cgtttacgag cgaatgggat ttgaccagga gtcaatcctt
    43801 ttgtatcttc aaacaataaa ccctgtttat ctacttgata gaaatgttgg cgtgcttctt
    43861 cttcaggtac accttgacgg atcatttcat ctaaaaggat gtttgcgata cctacaccag
    43921 cagtaccagc accaaatgtc aagatcgttt gatctttcaa ttgctcacct gatatattca
    43981 atccacctaa tacaccagct agtacaacga ttcctgtgcc ttggatatca tcattgaaag
    44041 tagtgatttt atcttcgtat ttttctaaga tcgttgcagc atttcctcgt ccaaaatctt
    44101 cccaatgtaa taacaattct gggaataatt cagtagctga ttgaacgaat tgatcaatga
    44161 attcgtagta agtttcgcct tctacacgag catgacgatt ccctaagtat aatggattat
    44221 tcaataattc ttcgttatta gtaccagcgt cgattgatac aggcaagact tgtgcaggat
    44281 tgataccagc cgcagcagtg tagaccatca atttaccgat agcgatgtca acaccgttca
    44341 caccccagtc acccatacct aagattcctt ctgcatctgt cacaacgatc aaacgaatgt
    44401 cgcgtccatc tgctgcgttt ttcaaacttg ctttgatatt ttctggttcg tcgattgaaa
    44461 gaaatgcagc atcttgtggt ttcaagaaaa tttcgttgta ttgttcgatt gaatccgcaa
    44521 cgacaggatc ataaacgaca ggcatgaatt caactaaatg ttcgcccatc aatttataaa
    44581 acaatgtccg gttcgtattg aatagattca ttaagaagat acgtttttcc aaatcggtct
    44641 gtttgcttaa gtattgaccg taagcttgga cagattgttg ttccaatgtt tggacagtac
    44701 ttggaagcat accagtgatt ccgtattttg cccgttcttc ttttgtaaaa gctgttcctt
    44761 tatttagaaa aggattattt aataagttta gccctgtaag catgaatgat tcctcctaag
    44821 ttttaataca aacgaagtat aggagtcaga tgaggatata gtcaaaaaga gcaggtataa
    44881 taaattttat ttatatgagt aatagagtca aaagaaatca aaatagcggt atttgttctt
    44941 tttatatcaa caatccttgc ttgattacta tgcgtatctc ttatatgcta aagaaagaaa
    45001 agtagaagaa taaaggatat gttagataga taaatagaat ctctgaagaa taaatcacgt
    45061 gatcgtgacg ataaaataca cttacgtcag gtgataaaaa atacataagt agaaaactaa
    45121 gaggaggaac agaaaatgaa agaagttcaa ttgatcagaa aaagcgaact atcagaaggt
    45181 ggttgcaatg catgcggcgt agtagaagca acaagctata cattaaagct tggtgccaac
    45241 aaagcgataa tctctgagtt gacagtaggc ggtcttgtgg acagtctggc gcttgctgaa
    45301 ggttttattg gagaagatat ttatgagatg ttttcagaaa tccgtcagtt aaaaaaaggg
    45361 gaaaactgca tagaggttca tcatgagagc ccgaatgtga gattcaaacg tggtgataat
    45421 gagatgatct tcaataatca tgtgtccgat cacaccgagc tatatggaat cgtgaatcaa
    45481 atactaactg agctttttgg attgggacct tatgcgttca aggaagaaaa cggtaatcca
    45541 aaattaaacg aagaatggca agaaacgatt gaaatacaaa gaaacaaccc gcacttattt
    45601 caataggcat ccacagtatt tcgtaaaagg gactttgaca cgacttttgt caaagtccca
    45661 tcgtttttta tctatttgtg ttctaaacat ttctttcaga gtagtaacaa ctggaatgac
    45721 tgaaatgtaa acagattatt tgcctgtatc aatacggaag agtaatataa agtgtgtaaa
    45781 cgctatcata ttattcattc aattttttat agagggaagg cattgaaatg gaaaaaacgt
    45841 atcaagcagg tacaaatgaa ggaatagtgg attttatcaa tatggaagat ttagaaatag
    45901 ccgcttcaca agtgattcca gctggtggtt atggttacat cagcagtggt gctggcgact
    45961 tatttactta tcaagaaaat gaacgggcat tcaatcatca gctgattatt cctcatgtat
    46021 taaaagatgt agaattaccc gatacaacta cacattttga caaagaaacg ctgactgcac
    46081 caatcatcat ggcaccagtt gctgctcatg gcttagctca tgtgaaggcg gagaaagctt
    46141 ctgcaaaagg tgtagcagat tttgggacga tctatacagc aagttcttac gcttcttgta
    46201 cattagaaga gataagagaa gcaggcgggg agaaagcacc gcaatggttt caattttata
    46261 tgagcaaaga tgatgggatc aatttggata ttcttgaagt ggccaagcgt aatggagcaa
    46321 aagcaatcgt attgacagcg gatgcaacag ttggcggaaa ccgtgaaaca gaccgcagaa
    46381 atgggtttac cttcccactg ccaatgccga tcgttcaggc ttatcaatca ggagtaggcc
    46441 aaacgatgga tgctgtgtac aaatcttcca aacagaagct tagtccaaaa gatgtagaat
    46501 ttattgctgc tcattctgat ttgccagtct atgtgaaagg tgtccagtca gaagaagatg
    46561 tctatcgttc attagaatct ggtgctggcg gaatctgggt atctaatcat ggtggccgtc
    46621 aactagatgg tggtccagca gcgtttgatt cgttgcaata tgtggcagaa gcagtagata
    46681 aacgtgtacc aatcgtgttt gacagcggtg tacgacgcgg acagcatgtt ttcaaagcaa
    46741 tcgcatcagg tgcagatctt gttgctattg gcagacccgt tatttatgga ttgtctttag
    46801 gaggaagtac aggagtccat caagtgtttg atttctttaa aacagaattg gaaatggtca
    46861 tgcaattagc tggaacacaa acagtagaag atatcaaaaa aataaaattg cgtgagaatc
    46921 gttttattta agaaacaaat gacgaaaaaa ataggagtgc ggcagatgat gccgtactcc
    46981 tatttttata gttcaaggat gtcactaata gaataatttg aaacgaaaaa ataaaattga
    47041 aatatttttt catacttaat aaaaaaggaa gaatattcat gtaaacgatt ttattgtttt
    47101 tttctctttt atacaaaaag ggtcataatt tcaagtaagc ggatgaattc atgaggattt
    47161 tttgcatact gtctctacta tacttaggtc actaagaaaa aggagtgatt tattatgaaa
    47221 aagaacatga ttgggacatt attactttca acacttcttc taggcggctt ggcaacacca
    47281 gcttttgctg agggtcaagc gacttcaaaa ggagatatta cctttactga accaacaaat
    47341 accgtggagc ctcttaaccc tactgatcct tcaaaaccag tagagccagc agatccggaa
    47401 aatccagcaa ctggccagac aggtccttta acactggatg tagttcccga attgccattt
    47461 ggtacacacg agattgaaag cggaacaaaa acgtatcaag tagacgcttc aaaaaacgat
    47521 acaccttatc tccaagtatc agatcgtcga ggtgtaggtg cagatggcca agcacaaggg
    47581 tggaacgtga cagtttcggt ttcagatttt gtaaacggtt cacaagtttt gcaaggcgct
    47641 gaattagatt ttggaacatc cactgttaaa agtacgtcag ataacgaatc cactggacca
    47701 actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag cgacaccaat atttacagca
    47761 gcaaaagatc aaggtctggg aacatggctt tctgtttatg atccagcaaa tatcacactg
    47821 aaagtgccga aagcagcagc tggaacattc acggctgatt tgacttggaa cctcgtggct
    47881 ggcccagtag catagagtat gaaataaatt caacaaactg cggtgaaaat actataggtg
    47941 aaggattttt taccgcagtt tttgttgaaa ggatgtggtg gacaatgaat caaagtaaac
    48001 gactgtttgt ttcgtttttt tctattttaa gtctattttt catttttccg tcaatcagcc
    48061 aagcaaagga ttcagcagga gactttggaa tcaaaccggt atttccagag aaccaaatag
    48121 ataaagcaat cggttatttt gatttattag tggcaccgga acaaaatcag acactagaag
    48181 taattatcag caattcttca gatgaagaac gaacatttga ggtctctgtc aatccggcgg
    48241 taacgagtga tggtggaacg atcgattata gtcaaaaaaa cccaacgtta gatgagacat
    48301 tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa ggaaatcaat gtatcagcac
    48361 atgcagaaac aactgtacct atagaagtga aaatcccagc aaaaagcttt aagggacgtg
    48421 tattagcggg tattcatgtt tcacctaaag aagaagctga aacagaaaat gctacagaag
    48481 gagcacaaat caaaaatcgt attgcttata atctcgcagt cgttttacaa gaaagtcaag
    48541 agacgataga accggatctg aaacttttat caggagattt ggacgaggtc aacgcaaagc
    48601 caactgttca gcttcgtttc caaaatcctc agcctaggat tatcagcaat ttgatattta
    48661 cgagcaagat cttttatgaa aatcagcttt atattgaaaa tacgtctaat gcatttttag
    48721 ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc tggtgataaa gcaaaagccg
    48781 gcgattaccg cgcagaaatc atagcaaaat caggcgacag taatgaatgg cgatttacac
    48841 aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa tgaaaattcc gtttttgcag
    48901 tacaagaaca atctttccca tggctttatg caggattggt tgctgcagtt ctagtgttat
    48961 ggttgatttt gctgtttttg tgggtccaca agaaaaagaa aaaaggagaa acaacatgat
    49021 gaggtgattt cgttgaaaac atcgatctta ttgggaggga ttattgcaag tttgctgttc
    49081 ttcagctggc aagagccagt ccaagcagat accaaaagtt caggtacgat tacattttat
    49141 gatctaagtc caattccgca tccaagtggt tcagtcaatg gcgcatcaaa acagcagaca
    49201 cagctaccga tttataaaga atctaatcaa catggcgtat tcccggagac gaatgaacaa
    49261 aagatccgtt ttctttattt gatcattggt atagaattat ggcttcttgc gtggttgttg
    49321 ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta caaaataaat tttcattttt
    49381 tcgctccttc ttttttactt cgctttttgt tttaagtata tttatctttt caccttcggc
    49441 agaagctgtt ggagcaacgc ctcctgccat ttcttatgat ccatacacgt atgaaaatat
    49501 cgtacctcca aatggtggac gtagtgcagt caatggcggg aagcttgtcc aactaactgg
    49561 ggctaaccaa ttgcctaaat tgactgctac ttatttgaac gatggttcaa atgatccttg
    49621 gaaaggtggc acggtccgtt ttcattggga aagccatgtg aaactaacaa atgtacttga
    49681 tttggtgaat atcactgcac aaggatttgc atttgtggcg atcatcaatt tgccggaaca
    49741 tgtatcaagc agtgatgtat taaaagcaat tgactggaac aacgcctact tgtatattgc
    49801 cggtgccccc atcaaggtta ctagcggaag tttattatca gcaggaagcc ataccttgcg
    49861 ttttgggatg gggtcgtggg atacaaatac tctattgaca gatattattg ctattattac
    49921 gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt gatgttacag ctaatgtcgc
    49981 gaagatgaca gaaaacggag ataaatatga tacgagtcca aacaaaattt tgaccaaggg
    50041 taaattacaa ccttctatgt ctaaaaaggc cgttttttct gttgattttt atgattcgaa
    50101 taatattatc caagatcgta tgttcaattc gattttagct ccagctgcag gtagatttta
    50161 tcctaaatta actggcggaa acttgaatcc aggagcatat gcattttctg caagtgcaga
    50221 gataaatacg tggaatagtt acatctcacc aagtgattca acaggaaaat ataatacatt
    50281 tgctgatacc acagaatacg tggatggaaa taaccagaac cttccaggct ctaccaatag
    50341 tcggacattg acgatggggc tgactgaagg acgttttgat cagttgccat ttagtcgctt
    50401 tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca caaggagcaa tactgactca
    50461 tactccagca agcacaagtg gattgaatca gacagccaat atcgtgtaca gtgggaaaga
    50521 tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta ttggagcagt acaagctaga
    50581 cggaacggtt ttaccaacaa acttaacagc cgatagtcag tggggaaagc tcctttcttc
    50641 tgcatcgtac caagtcacgg ccaattggtc agcaactgaa ctaaaaaagg gttcgatcca
    50701 ttacaggctt tattcgaaag gaacccaaaa attagcgaca ggatggacag atcaactttt
    50761 ccaaacgatc caatcaaata acgggggtgt aaatacagct acttctacac tacctgcatt
    50821 ggcttcaggg caatattatt ttgattatcg cttggtggac gatgtactta ctaacatgta
    50881 tccgtctttt gcgtacaaat ggcagtctga acaagctgga atcaccactc ttcctcaaat
    50941 aacagtggct aattttccat ctgtgagtgc agatagtcag ctaaaaaacc ttagcagaca
    51001 acctgagaca ggagatcccc tgtctgctct atcaggagat acgattcaag aaaatgtgac
    51061 attcacgtta acaaaatctg gggatgcgat aaccgacaaa aaaatcacga tctcacttcc
    51121 tgaaaatact acttatatca atggttcctt aaaattaaat ggtacaacta tcgcagatac
    51181 cggcattcaa caaggagttt ctgtcccagc agatctattg agcaaaatag gagataccat
    51241 tcatcttact tacaactatc aattaaatac tgtagatact tctttacaat ccgtcagcat
    51301 actgacaaag gcagctgtac tttccagcaa tattacatta gcagatggtg ctaagcttcc
    51361 taacccagtt gtccaaacat ctgctaaaac aattcttgtc cccaaacaag aattaacatt
    51421 agttaatgta ccagatgatt ttacatttgg taacgatctc cccaaaccgt tgaagacaag
    51481 ttattatgaa tcaaaaggcg atttttcttt tgatgtgcgg gatactcggc ttccaagcac
    51541 gtctccttgg caactgacag gtacattgac ttctttattt aagaatgatc aaggtcaaga
    51601 attaagcgga acaaaattat acttcaatca ttctggatca aagcaattga ttcagcaagg
    51661 tcaaaacacg ttgatttatg agagtgatgg aacagctaaa ggagaggttc tcgttgactt
    51721 tcccgatacc gatggtttac tcttagaggt aaatagcagt acgaatgctc aacctggcgc
    51781 aacgtatcaa ggcatggtca catggaaact gacagctggg ccaaccagct aagtgattag
    51841 aatttgtatg gatctgtgat aactgtcaca gatcctcttt ttcaaggatg agaaggaaac
    51901 ttctgctgtt aaaatcagaa atgcttgaag gagtggactt ggaacttttc agtttgcttg
    51961 aaaaaaatga acaacggcaa atcaaacttt tccaactttt attagaggaa aaaggagagg
    52021 tttccgttaa taacgtactt acccgcttat cagttgatcg ggctaccttg aaagaagatc
    52081 tgttttattt acaagctaac ttggaaaata tcaaacaatt ggaaattgtt atccaagaag
    52141 ggaatatttt attaatcaga catggaaata tagcagcagt cgatgtttat tatcattgtt
    52201 acttgcgcta ttctacaaaa tatcagatac ttctttatct gttggaaaat ggctcatttg
    52261 atcggcagaa acttgtcatg gaacttaata tcagttcggc tactttgtct cgcagagtga
    52321 aggaattaaa tgattcttta aatgagtttc atctgaaaat aaaaagcggg cggctgaccg
    52381 gtccagaaag tcaaattcgt tatttttatt tccaattatt ttggtttgga cgtccctatc
    52441 ttgaaaacaa aaaagaatac agtgatttat caggaaacac ttttttagat ctgctgcaaa
    52501 aagagtttag ttttgctatt acagaagaag gacgattgaa atttgaaata tggctttcca
    52561 ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc agattatagc aatcttcctg
    52621 ttggattgtt tcaaacgaca caatttttat tggacctcca tacatttttg acccgttact
    52681 tctttcaata tgcctttcct tggaatcaaa atgaaacgtt ggttttttat cttttcatga
    52741 tcagtaactt tacattagat acacataact cgcatgtcca ggagtttttg ataagcgaaa
    52801 aatacaaaga cgacgtagta gctcaattga atcgaacgtt taaacaatcg atagaagaat
    52861 atttctcttt ttatgttttt ccagaagaga tttcgcaaaa gattgcgttg acagtgatgc
    52921 aaaatcacgc tcattttgcc tattttagag gatggatgaa tatttttggc caagaaggat
    52981 taaaacagcg gttaactgaa gtagaagata aacaattcct ggttttatgt gaaagattgg
    53041 tcagacaaac atttgctatt gttgatttta ctgaagtaga cgaggaaaat gcccgctttg
    53101 aattatttgc tcgctatgca tctattttac ggacgctgat caagcagtta gaattacgtc
    53161 tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc ggatatcgtg aaagaaatca
    53221 tccaggaaca attgctgaat acgatcaaat atgatttagt cgaatacgag aaagaaaagc
    53281 agtatgatat cattttgact agccaattga aagagtaccc gtcacagaag tccgctaaag
    53341 tttttgtatt cactagcaac gaatataatt atgatttccc ctatttgaac caatttttaa
    53401 aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa aacatgaaaa tcgtgatcat
    53461 tggtgcttcc catgcaggca tcacagctgc cctaaatcta agaaaacttc aaccgaaagc
    53521 agaagtttta ctgatcgatg aagatcataa agatggacta ggctatgttt caaatggaat
    53581 caacctttat ttaaaaggaa agatacgtag tttagctgaa gttgcccata acatgcggac
    53641 tcttcaagaa agtggggcaa aactgatcac cgaatggaga gtaactgaac ttgatccaga
    53701 caaacatcag ctcatcctcg catccaaaga aggaaaaaac gagaccataa cctatgataa
    53761 attaatcgta gcgacaggtt cctctcctgt caccttgtat aaacaaattg aagctgaaaa
    53821 tgtctacacc tataaaaatc tagtgcaatc taagcaagta cttgccgctt taaaagaagc
    53881 aaaagaagta gtgatcttcg gtgctggtta cattggattg gaattagcag atgctttgag
    53941 aaataaagga tacattattc atctagttga ttatatgccg aatgttttat caagatattt
    54001 cgataaggat atgatcaaca gttttcaaaa tcagctgcaa actaagcaaa taaattttta
    54061 tccaaatgaa ttcttgatcg attggaaaaa atcagaagag aaagtagtat ccgtacaact
    54121 tctgtctcaa acaataaagg cagatatggt cattttttct gcccaaactc gtcctaatac
    54181 gacattgtta aaagaaaaag tggctttgta tgaggatgag actgtgatag tcaatgaata
    54241 tctgcagaca agtgatccgg acatctatgc gattggagat atcgtcccag tcagttttga
    54301 caaaaataaa cggcatctct ttttaccttt agttactcga gctgtacata tggcaagagc
    54361 agtagcgcta actttgagtg gtcagccaac cgcttacgac ctcagacaaa agataacagc
    54421 agcagtcatt atagactact ttttaggaac tgtagggctg acagaagatg aagcgccatt
    54481 cttggaacaa aataccggtt cgtgttcagg agaatttgat ctatttccac aatacgacga
    54541 agaaaacaaa acagtaaatg caaagctgat ctaccatccg gatacattag aaataattgg
    54601 cggacagctg ataagccaag aattcttatt atcagatttg aacctgcttg cggatatcgt
    54661 gaaacacaaa actacgattc cacagctagc agttgaggat tttggatttc tgtctgaata
    54721 cacctctcgt tttcactacc tcaatgagtt agcttttaaa gtactagcta agagagcaaa
    54781 tagcaataga gtagataaaa gactgtgaga aatatcacgg tcttttattg tgtggggata
    54841 taataaatat taatcaatcg agaataaaaa attcatataa tgaaatagcg gaagaatttt
    54901 ttgagcataa tattcgccaa aatttatcaa gtgataaaat tgttgtataa aatgattatt
    54961 tcaggaggag gagttaaatg tttttagcat taaatgaaat tatgcattca aagttacgtt
    55021 atgcgttagt tgcaggggtg atgtttttga tcgcctacct ggtatttttt ttgactgggt
    55081 tagcttatgg cttagctcaa gataatcgaa cagcagtcga caagtgggag gcagattcga
    55141 ttgtgctttc gaaagatgca aattcaaatc tagggatgtc catgattact aagaagatag
    55201 cagaagaagt cgaaggtggg aaagtggcgt atcttgccca aacacctgga gtcgtgacta
    55261 gtaaagacag tacagaagaa ggaaaaatca atgttagctt cttcggtatc gataaaaatc
    55321 aattcatcat gcccaatctt gttgaaggaa aagcttttga taacgatgat gaggcagtag
    55381 gcgatattag tttgaaagaa gaatatggtc tagccatagg agatacagtt aagttatctg
    55441 gtagtgacaa gacgtttaaa ctaactggct ttacggatca tgccaaattc aatgtctcac
    55501 cagttctcta tacaaccatc aatgcttacc aagaaatccg cttcgaaaaa gaagatacaa
    55561 gtgaaaatgc acggattaac gcaatagttg tcagaggtaa aatcagtaat ttacctgaag
    55621 atttagaaca gatcaaaatt tctaaattca tcaatgaatt acctggatat aatgcgcaag
    55681 tattgacttt tggatttatg atcggatttt tgatcgttat tgcggctatt gtgatcggga
    55741 ttttcattta tgtactgacg atgcaaaaga ttaatatctt tggtgtaatg aaagcacaag
    55801 ggatcacagg aggatttatt gcgcgttctg tagtggcaca aacattcatt ttatcatttg
    55861 ttggtattct tcttggtcta ttaggaacag tgggaacatc ccttgttcta cctgatgcgg
    55921 ttccattcca aagcaattgg ctgttttttg gcgtcattag cttgttgatg ttagtagtag
    55981 ctgtgttagg cgcactattt tctgttcgga caatcgtaaa aatcgatccg ttgaaggcaa
    56041 ttggataaga aggaggaaga acatgaaagc aatagaattt aaagaagtag aaaaaagttt
    56101 ccaagatggc gaccaagtag ttaaagcatt gaaagaaaca accatgagta tcgataaagg
    56161 ggaatttgtt gcaatcatcg gtccttctgg ttcaggtaaa agtacttttt tgactttggc
    56221 aggcggattg cagacgccta gcagtggctc tgtatttatc aatgatcaag aattcagtaa
    56281 tcaaaaagaa agcgaacgcg ttaaactacg ttttaaagaa attggcttta ttttgcaagc
    56341 ttccaatctg gttccttttc tgacagtaaa aaagcagtta ttattagtag ataagattaa
    56401 acatgaacat cgtcaaaaag aagcaaacga attatttgag cagcttggcg tggataagtt
    56461 gatcaacaag tatccagaag aactatctgg tggagaacgg caaagggtcg caatcgctcg
    56521 tgcattgtac aatgatccgt ccattattct agcagatgaa ccaactgcta gtttggattc
    56581 agaaaaggca cgagaagtag tcgatatctt ggcaaaagaa tcaaaagaca aaaataaagc
    56641 aacgatcatg gttactcacg atacacggct gattgacaaa tgcgatagaa tatttatgat
    56701 ggaagatggt gtattgaaag aaaaagaata accaaaaagc aggtggaaaa atggtccatc
    56761 gatacaacaa agatacacag caaaaaattt tgaaagaagc gactgacttg tttatgtcca
    56821 agggatatct cggcacttca acaagagaaa tcgcacaaaa agttgggatc acccagccca
    56881 atctctatca ttatttcggt gataaagaaa aactatatac ggcagtttta gaaaaccact
    56941 taaaagatgt tggaaaagct ttgagagaaa tcgttcaaac aagcgaaacg ggatttcaag
    57001 taaccttgac aaaaatggcg cagtttttga ttgagacaca tctggtcgat ttgtttctga
    57061 tgcttcacga tcttgaaagc aatctttcaa aggaaacaag agatcatctt ttctttctat
    57121 ggaagaaaaa ttatcgtgaa ccttttgaag aaatcttttc agcaaaccaa tctgtgctaa
    57181 gaaatgggat ttcaaaagaa atcgcagcaa ggcatttttt cttagtgtta gcaccttaca
    57241 tcactcggtc gaaaagacct gtggaagaac cgctaaatgt ggcgcaattg atcgatttat
    57301 atttgaatgg tgtattagga gaagcgtaaa gctgttatat agggagaaaa tagttaggtg
    57361 aagaagcaag atatgacgaa gatcgttata tcttgcttct ttatataggt agtttttcat
    57421 aaagacaaat ttgttattct ctcatgatta tcagggtata tttttattta ttatgtcggt
    57481 gttccaggaa gtgttttaat ttaataacga agaaattaat tgtttgtaga atgaaggaaa
    57541 ctatttattg ggtagatgaa tcaccacgaa tagtcaaaag gagatttcag gatatgccaa
    57601 aaatgaaaaa aatcatttta gcagtaatgt tattttctac ggttatatct tttacagccc
    57661 cttttttgct tacttaagct gattttaagt tttggccctt agtctggatc tacgaggtga
    57721 gagtcaagaa tgaaaaaaat aattggagct tttgtatttg caatcattat cattattatc
    57781 tgttttcaaa tagatgtttt tggtcaaaaa aagatagttg gacaaacggt cagcagtacg
    57841 aacagtacgg ttacaaagac aaaggaatca tttgatgata cagaacaaga gacattaatc
    57901 aaaatcggaa aaaatgaccg tacactatat acagatgact cgctaactac cccacttgca
    57961 actataaacg gcggagagct ggcggagttt ctttctgaaa caaaaaatgc ttatcagatc
    58021 aagacaaatg atggctatac tggatattta gccctcgaag atggaaccaa agtcacaaag
    58081 aatatacaaa caaaaccgga agagctatct gatgcagtga ttgtattaga tcctgggcat
    58141 ggcggaaatg atactggggc attaagcaat gatgaacaaa cagaagaaaa agatataaca
    58201 ttaagtacag ccataaaagt aaaaaaggcg ctagaggatg ctggagcaac ggtttatttg
    58261 acccatacta cagatgaatt agtacagctt ggagatatat gcgactacag tgaagaaaaa
    58321 aaggcagatg tctttatcag tttacacgca gactctacgg aatatgcgaa tgaagcaact
    58381 ggaatcacga catattatta ctatggacaa gaggaaacat tagcacagac aattgctgac
    58441 tccttcactg atctgcctct ggattcaaga ggaatctcca ctgggaacta tcaagttctt
    58501 cgagaaaatc ttcaaccttc tattttaatc gagatgggtt atatgaataa tgatagtgat
    58561 ttaaaggaat tagtaacaga cagttatcag cagcagattg ctgcaagttt gacacaagca
    58621 ttgaccactt attttcagta ataaataagc ccagccaaac aaaaatagct cctcatattt
    58681 ttcgtttggc cgggcttatt atcgcagatg caagcttttg aacaccgttt atttttttag
    58741 tagcttcaaa gctttttctg tatttgcaaa gtgaagtttg gcatattttt caagcatagg
    58801 catgccgcct ttctccaata gttttgccgc tacttggtca gatttcgttc cagctcctga
    58861 tggaagggtg attccagctt cagctgatgc tttgtgtaac tcttccaaaa agctggcacg
    58921 actgatgata gaagcagcag caacagctaa atgatattgt tctcctttag taacgaagta
    58981 cagtttttct tgtaattgat ttttttctaa acgcgcatat tttttaaaat ttgcttccgg
    59041 cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc tcgatttttt ttagcagcaa
    59101 atggatcgct tgattgtgca aagcgacttt catccgaacg gcattgtatt ttggttggat
    59161 ctcattgtat ttttctggag ataagacaag cagtttatag gggatggttt tttttaagat
    59221 gtcagcaagc tggatgattt ggctatcttt taactctttt gaatctctaa caccaagttt
    59281 acgtaattct gcaagctgat cacgtttcac ataagcagca catactgtta gcggaccgaa
    59341 atagctgcca tttccgacct catcactacc taaaacggat aattgactga tattttttgg
    59401 caaagaagag gctgtttttg ctggcttggc ttgtttatcc tgtgcctccc cccatttatc
    59461 agcttctatt gctgcattcg taccttgaaa catcactttt ccggatgtat aagcggtaat
    59521 ggtcgtcccg tctttttttg cagcaaagct agtataagga atctgtttat ttaacaaata
    59581 agagcgatag tatgctttca tttgactaag ttggtcgttt gttgctttga taatgacgtt
    59641 gctcataaaa ttctcctcgt ttttatacat aaaactatac cgcaactcac gacttttcgt
    59701 tttctaagag attgcgagac atagcatttt tttaaagttt atcatataat agttaagtat
    59761 gtgaaaaata tttgtggaga acggtgaaag aaccaacttc atggtacact aaataaaact
    59821 ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa aaacacgcta caaagcggtg
    59881 attgccaacc aaacatatac tattatcgga cgggaaacca agcatcatat ggatatcgta
    59941 acgaagctga tcaatgaaca gttagcagaa ttgaaacaat tatcaccaca aatggataat
    60001 gaacaagcag caatcttgat ggctgtcaat gcgctttcgg atcaattgaa gaaacaagaa
    60061 agaatcttgg agctggaaga agaaacagca gaattaaaga aaaaaatgat caaatttaca
    60121 gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga acgaagctcg ggaagtattg
    60181 aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg tggaagcaca acagatttta
    60241 aacgagaaac gaaaaggaca aattaaacaa aaatcttcta attaagatcc ttttatcaaa
    60301 ataagaaaaa tttaaaaaaa gttagaagag aaaggaacgt agcatgctga gtttgctgat
    60361 tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg ctttgcaaat
    60421 catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt acaaaccatt
    60481 agcaagtcat ttggaattat acattccata cccagctgtc acgccaacat caaagttagc
    60541 attttttgat caggtatttg cctttcacct agatgaagct ttttatgcag gaactgcttt
    60601 tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt ttgtccatgg
    60661 attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca tcctgtcact
    60721 aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg ttccattaga
    60781 tgtggtccaa aatcaattcg gcgagcatag tttcgctcgc tttatcgttg agagaacgcc
    60841 atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt aaggaaaata
    60901 gtgctggaaa atatttcgtt ttttgtacag agtagattat ttaagaaggc tgcgtagcgt
    60961 atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga ataaccgaat
    61021 ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg tagtgactgc
    61081 acaaggaaaa gaagaactgg cagaattggt accagtgagc gaggaacaaa cgatcacaaa
    61141 ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg ggatccctat
    61201 acctaaactt gaaaatatcc gtccccatat gaaacggatt gagatcggag cagacttgaa
    61261 tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat tgaatcgttt
    61321 cattgatgat ctttatgaca gtgaaatcga atttgcacgt ttgtatatgt gggcagatca
    61381 gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg aagacggacg
    61441 cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc ggcgaagtga
    61501 acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa agtatttaag
    61561 tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac aagaataccg
    61621 cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggacagactt tatttattga
    61681 accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg ctgaacgaaa
    61741 tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc gtcaagaaat
    61801 catccacaat gcttatgtga ttggtaaaat ggatctgatg aatgcaaaag cacgatttgg
    61861 taaagaagta aaagctatcg tgccgggaat cagtgaagac aatcatgtcg tcttgaaaca
    61921 agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca caatcggaaa
    61981 agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga tcacgttgaa
    62041 gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg caggtgaaga
    62101 aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac agtcgattga
    62161 gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt tatcaaaagt
    62221 caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc cgcaagaagg
    62281 tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt atgtgatggc
    62341 aacaacgcat tatcctgaat tgaaagttta tggatataat cgggcaaata cgattaatgc
    62401 cagcatggag tttgatgtgg atacattaag tccaacgtat cgtttgctga ttggcgttcc
    62461 tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag aagttattga
    62521 tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga tcacagattt
    62581 agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt ttgtttctga
    62641 ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg aagaacgtga
    62701 aaaagaaatg gaaaaagcaa agaaaaaagc aaatgaagtc gtgtcagagg cggaagaaaa
    62761 agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc aaggaaatgt
    62821 gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc aagaagaaac
    62881 tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga aaccgggaga
    62941 cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa acgggaatca
    63001 gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc tgacgcctgt
    63061 tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg aatctagtag
    63121 tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc taagtgaggt
    63181 agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta ttgtccacgg
    63241 aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc atcggagcgt
    63301 caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa ttgttaaatt
    63361 taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga aatgctatac
    63421 tagtcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac agataagaca
    63481 tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac ttggtgtggt
    63541 ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga tgaagaagaa
    63601 ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca attcggtatc
    63661 atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa agctgttggt
    63721 gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa acttacagca
    63781 aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg cggttgagtt
    63841 aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg attgtttttt
    63901 tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga tgaacgaacg
    63961 aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga tgaaagataa
    64021 aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg tccgctcata
    64081 ttttactggt agccataata cgaaaacgga acggcttgtt agtgaaatcg aagactttga
    64141 atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc tgataaagaa
    64201 aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt tccttaagat
    64261 aacgaatgaa aaatatcccc gtttggtgat tactcgtaag gtactaaaag acaaagcact
    64321 gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga tcctcgatcg
    64381 attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc tttactatca
    64441 tttgggacaa tgtctgtgtc cttattattt cgacgtagat ccgaaagtct atacgaaaat
    64501 cgttgatgaa gtgaaaaact ttttaaacgg aggacacgaa aaaatcgagt cagagatcca
    64561 tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag aataccgaga
    64621 ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata ctgatttact
    64681 ggatcgagat gtatttggtt atgcgattga taaaggatgg atgtgtgtcc aagtgttttt
    64741 cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct ataacgaagc
    64801 agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc atttcattcc
    64861 taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt tgttagctac
    64921 gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg cgagtaagaa
    64981 tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg aacggacgat
    65041 cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac gtatcgagtc
    65101 ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg tattcattga
    65161 tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca acggaccgga
    65221 tgattatgca tcaatgagag aagtcatcta tcgcagatat tcgcgagtga ttcgcgaagg
    65281 gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag atgtggcaaa
    65341 agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg caaaaaacga
    65401 taaacataaa acaaatgagt tgctattcgg tacagatttg gctgtagtac cgcttgagcg
    65461 aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc gattcgcaat
    65521 cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag atgaaattga
    65581 agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt tgaaaaatat
    65641 cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg tcgcacaaaa
    65701 cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa agtaaagtgt
    65761 cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat aataaatcgc
    65821 ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag aaatgttaga
    65881 agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg tctctttcca
    65941 aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga agacaacgac
    66001 ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat ggaatggcca
    66061 tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac ggggacttta
    66121 tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag ggaaatcaag
    66181 aaaagagatt gaaccaaaaa tcgacttttg gatggaaaaa tttcaggtaa aagggaaaaa
    66241 gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc tgatcgccac
    66301 gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt tagatccggt
    66361 aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt catgtgtcat
    66421 tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga tcatgttgcg
    66481 aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat ttggccgtac
    66541 gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg aaggcgtgaa
    66601 aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc ctgaagctgg
    66661 gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta accagcaacc
    66721 gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt aagttttgga
    66781 ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg atcatgatat
    66841 tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct caacaaaata
    66901 gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag caaatgagcc
    66961 aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa gcacaaaaag
    67021 agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat gataagataa
    67081 atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg atacaacaac
    67141 aactgactgg cttgcaatcg atgcttcgag cgagtcaatt aggcatttca ccagaagaag
    67201 tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt gattcaaaag
    67261 gaaaaatgac agtcggagaa gataactcga cagtccaata cgtcgtaagt tatgtagcaa
    67321 cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa gagatcgctt
    67381 ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca caaaagcatt
    67441 tttacggaaa attaacaggt gttttacttg ttgcattaac gcaaatggca ttatacggta
    67501 ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa aatatattag
    67561 acggtatctc attggataac ttgattggac cattcttgtg gtattctctt ctattcatga
    67621 tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg gtcaacaaag
    67681 cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc ggatacatgc
    67741 ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc acttcgtata
    67801 ttccattctt atcttcatat attatgccgg ttcgcttagc aaatgagaca gtcggtatgg
    67861 gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg atgatcttgt
    67921 ctgcaaacat gtacaaatcg aatgtactcg tttatagcga aggcggatta tggagttcat
    67981 tgaaacaatc gatttcaatt atgaggaatg aacgaaaaaa agcataaaac aatcctaaaa
    68041 tacagaagga agctggacat ctattgttcc cagcttcttt ttgtatagac ctattttatt
    68101 tattttacta tattggatat atattcattg aaaatagtgt ataacaactt tataatattg
    68161 agtataccaa tttttggagg cgttttaatg gaccgttatt tagatttact ttcagaaaaa
    68221 ttcccctcaa cggaggatgt catcacggaa atcatcaatc tcgaagcaat tttgcagctt
    68281 ccaaaaggaa cagagttatt tttgagtgat atacatggcg aatttcctgc ttttgatcat
    68341 attctgcgta tcagttcagg aaatcttaaa gaaaagatca aagacctttt ttctgaacaa
    68401 atgacggaaa cggatatcag ccaattcact atttttacgg cttatccaga atatgcatta
    68461 acgactgatt ggtacaaaga acaagataaa tcgcagctga tccatcattt gatcgatctt
    68521 ttacggttta cgacagtcaa atatacccgg tcaaaagttc gaaaaggatt gccgaaagaa
    68581 tatagctata tcattgagga actcttgtat atcgatgacc gtatcaatgg aaaaaaagac
    68641 tatgtaaaaa aaatcattga acaacttgtt ttgatgaagg aagaagaaaa atttttaacg
    68701 aaacttgcac agacgatcca aaaatctgtc atcgatcatt tgcatatcgt tggagatatt
    68761 tttgaccgag gaactcaagc agccaaagtg atggatcgaa tcatggattt ttcttctgta
    68821 gatatccaat ggggaaatca tgatattttg tggattggtg cgtattgcgg ctctgaggca
    68881 tgtttactga cacttttacg gattgcagca cgctataatt atttatttga attagagaaa
    68941 gaatatgggt tgaatttacg tccattgttt tcttttgctc atcaaactta tcagaagaat
    69001 ccagtatttg cgcctaaaaa cagtaaaaat ctatccgaaa gagagcaaga agaattagaa
    69061 aaaatccatc aggcactttc tatccttcaa ttcaaatcag aacatcagtt attggatcgt
    69121 cgacctgaat ttaaaatgga cgatcgtaaa ttgctggaaa aaatcgacta tgaagcatca
    69181 acgattgatt tagaaggtca gctctatgag ttgaaaggaa cttgttttca aacgatccag
    69241 ccagatgatc caaaaaaatt cttggaagag gaacaaaaag tgattgatag tttgatgtat
    69301 tcattccagc actctttgag aatgcaaaaa catatgacgt ttttgatcga aaaaggggga
    69361 atgtatctca cgaacaatca gcatgtgctg tttcatggct gtatacctgt agacgagaag
    69421 ggacagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact gctgggcttt
    69481 tttgaaaaac agataactga aagcgcaaaa gatctgactg caaaagaaga tttagcaacc
    69541 gatttgattt ggtatgcgtg gagtgggcca ttttcgcctt tattcggcaa gagtaaaatg
    69601 gcgacttttg aacgttattt tctcacagag cctcatacgc acgtcgagaa aagcaatcct
    69661 tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttagcaga attcgacaca
    69721 aaagaagaag ggtcagcaat cattaatggg cacacgccag taaaagtaaa aaaaggggag
    69781 tcaccgatca aagcaaatgg aaaagtattt gtcattgacg gaggtttatc caaagcttat
    69841 caaaaaacga caggaattgc cggttattca ctattatgca attcttatgg tttccaaatc
    69901 gtgacacatt atccgttttt gcctattgaa caacaatttg caaaaaaatc cgaccaaaca
    69961 aatgtgaaaa aagtaatcga acagcaacta ccacgcaaac tcaatcgtga tacaacaaaa
    70021 ggtatggaac tgcagcagca gattacccag ctcaaaaggc tgttggacta tcgaaaggac
    70081 gattgagaag aattccagtg aacattgttc catagactgg agactgagac aaaagtcgtg
    70141 tcgcagtctc tttttccgga taaacggtgt tcttctgcca actcctcggt cacaagtcac
    70201 actattcaac aaacatgaaa agctgttttt tgaaattgct ccttgtgact cagagttgaa
    70261 cggcaggctc acgacctctt ggtataaacg gtgttcaaaa gccagtttct cggtattaag
    70321 ccaaatttat gcaaaaatgt gaaaagccat tttcacaaaa tttttcttaa tactcgaaac
    70381 taaacagctt tttcacaacc ccctcagaaa gtctattaaa attcattcac aaagaaatcg
    70441 aataagcgct gttcagatgg atgtacacgg tgggtaagtt ccggatgcca ttgaacaccg
    70501 aggattttat gcatatcatc tttgctttga accgcttcaa tgagtccatc gttagaaaaa
    70561 gcgatggctt ttaataaagg tgaaagatct ttgactgcct gatgatggta agaattcaca
    70621 aaatatggat cggggagaag gttgcttaac aaactgtctg aaacaatctt gacttcgtga
    70681 gtcgtaaatt gcggggcagt gggctgttgt tcatgtttta ctgaccactt tggatataat
    70741 gaaagatctt gataaagtgt accttccaaa acgacattca atagttggag tccccggcag
    70801 actgcaaaaa ttggttttct ttgtttcaat gcttccaaga tcagtgcctg ttcaaattgg
    70861 tctcgttgaa tatttgtttc cgttagcttt ggatgaggat cttccatgta aagttgtgga
    70921 ctgacatctt gaccacctgc aagaataagt ttgtcgattt gtccaatcag ttgaggagct
    70981 gaatctggag acatgactgg cagaataaga ggcaatcctt gagcatctaa aactgcatcc
    71041 acgaatcctt gcggtgtata gctcacttga tttccttgga aagtatctgt tgctcgaatc
    71101 agttgatttc cggcaatgcc gatgattggt ttcatttgta ttccccctaa agtagactta
    71161 ttgtaacaag gatttaaaaa aaattaaact atgtaagact tgcccctata cttatctgtt
    71221 cttgctgtgt tactatctgt aaagagaaat ttcgtgtgaa attcatgacg atagttgaaa
    71281 atattgctta atttgttata ataaaaagta agcagaaata aaaaggttgg gcgtaagctt
    71341 caaggatcta gttcttcaag gggtgagaag gacattttat tgaaggggcc tgcctatact
    71401 aagttatctt tagaggtaca tactttcaat gaaaaaagaa agcatgtacc tcttttttaa
    71461 atcgaaagga agtgaagcaa gtgttagaag ctttggaaca aatccgaaaa gcagaagaag
    71521 aaaatgaaaa gcgtgctcaa acactgcggc agcagctgaa gatctatgca acagaaaaag
    71581 agcaggaatt ggaaacgatc caaacaaact atcgacaaca actaaaacag ttggtggaag
    71641 aaaaagaaca atttgaaata caagaattag aaaaagaaaa aatgaaattg aaagcagaag
    71701 cagacaaggt gcaagcaact ttgaaagatc aatatcaaaa gcatcagagt caagcgatcg
    71761 gtgcgatcat tgaaagggtg aaagaaactt atggccgtca ctaagatgga aaaagtgacc
    71821 ctcatttcag ataaaagaaa tcaggagagg attttacaag cagtacaagg aatccaaaac
    71881 gttgaaatcc gggatctgta tcaaaataga acaaacaacc agtgggtgga gaagtatttt
    71941 ccagatacca cgattattga taaagaagca aaattaagcg ctttaggtaa tcggttgaaa
    72001 gacatccaag aagctatcca attcatacaa caccacggcg atagccgtca aaagaaactc
    72061 catttgaaac gtcaagaact ttccttacaa gatttggaag ccaattattc cgaagaaaat
    72121 ttcataaata aattgaaaga agtccttgat ttaaaagaaa agtgggaaca actaactgaa
    72181 gaacgtgatc gtttagaaga agaggaagaa tggcttcttc attggcaaca gctggatatt
    72241 gctccaagta tgtatgcaag taagacgacc atttttgaga tggggactgt gagtgcagca
    72301 aattttgaaa gtttcaagga agaactgagt aagcttgatg acgtttatat ggaagaaata
    72361 gattatacgc caaaacaagt ttatctttcg tatgttgctt taaaaaaatc agttgctgtt
    72421 atagaagaaa ttgctgggaa atacgggttc aataaagaaa cgtatcctta tgaacaaaat
    72481 cctaaagaaa aactgcaaga gaccaagcag caattatcca ttgttcacga gcagcaaaag
    72541 aagataacga cagcgttagg tcagtgcagt ggctatatcc aagaatttga atgggtagaa
    72601 gaagtcacac ttgccgttgc agaacgagaa aaaataaaag aacggtttat ccatgcgact
    72661 tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa aacagaattt gctgcatgtg
    72721 ttaaacgaaa ccgtatcaga agaagatata tacgtcagtt tcgaaacacc aaactcagaa
    72781 gagattcagc aggaagtccc tactaaattg aaaaaccatc cgcttgtcga accttttgaa
    72841 ttgctgactg aaatgtacag tctgccaaaa tatgaagaag tagatccaac accttggatg
    72901 acaccatttt atcttgtctt cttcgggatg atggtcgcag atgttggtta tggtttgctg
    72961 atgctcattg ggacaatact agctcaaaaa ctacttgttc taccacgcgg gatgaaacgt
    73021 ttcgtaaaat tctttgagat tctctcgata ccatcgatca tctggggatt gatctacagt
    73081 tcatttttcg gacagtcatt accaaaagaa ctgtttggca tccgcttgcc tttcccaatc
    73141 ctatctacga cggatgacgt caatacgatt ttgattttat ccgtgatttt cggtctgata
    73201 cagattttgg ttggattatt tgtggcagca aaggaaaata tcaggagaaa agattacttg
    73261 agtgctgtca gtgatggatt tgcctggcaa gggatcctaa ttgggatcgt tattgcgttg
    73321 gttggtgcca tgttgataaa aaatacagca tttgtttacc ttggcgctgg gctatcgatc
    73381 attgctgcct tatgtatctt gatcgttccg attatccaaa cgccgtctaa agtcaaaggt
    73441 gctgccaaag gtgcgtataa tctttacgga ctgaccggct atatcggtga tttagtcagt
    73501 tatacacgtt tgatggcatt gggtatttca ggcggaagta ttggagcggc attcaatatg
    73561 ctagttgctt tcatgccgcc agctgctcga ttcagtgtag ggatcctttt gatcatcgct
    73621 ttacacgcac tgaatatgtt tttgacactg ctaagtgcct atgtccatgg cgcacgattg
    73681 cagtatgtcg aattttttgg gaagttttat acaggaggcg gacgtgcctt tgatccgctg
    73741 aaaacggcgg aaaaatacgt caacatcaat cacaagaaaa aaaacaataa ataattggag
    73801 gatttttata taatgatgga ttacttaatt agtcaaaatg gtggaatggt atttgcggta
    73861 ttagcgatgg caacagcgac gattttttca gggatcggtt cagctaaagg tgttggaatg
    73921 acaggtgagg cagcagcagc attgacaaca agtcaacccg aaaaattcgg gcaggctctg
    73981 attctgcaat tgcttccagg tacgcaaggt ttgtatggat tcgttattgc ctttttgatt
    74041 tttatcaact taggaaatga catgtcagtt gtacaaggac tgaactttct aggtgcttct
    74101 ctgccgattg cttttacagg cttgttttca ggtattgccc aaggaaaagt cgcagcggca
    74161 ggtattcaaa ttttagcgaa aaaacctgaa catgctacaa aaggaatcat ttttgctgcg
    74221 atggttgaaa catacgcgat tttaggtttc gttatttcct tcctattagt gttgaatgcc
    74281 taagcataaa ggaggaaaag cagatgaacg ccattgaaaa aatcatctct cagatgaacg
    74341 aagcagcaga gcaggaaaga gcggcgttag aacaagaaga gcgaatgaag atcgatcaaa
    74401 actttgaaca aaaacgtacg caagttgaaa cagaacatca aaaacaaaaa gaaaaacaaa
    74461 tcgaattact cgaaaaaaaa taccgtcagc taaggaatcg ccagcaagta gaagtacgac
    74521 aagaaaattt gaatgcgaag caggaatttt taagacgatt gtttgcagat gcggtaactg
    74581 aaatggaaaa ttgggatgaa tctgaacaaa tccagtttat caaaaatgca ttgtattcat
    74641 tacctttgac aggaaaagta gcattcattg ctggagaaaa atcagcagcc tatctttctc
    74701 agacgttgtt ggatgaatgg aacaatgaac ttccctttat gatggtttta agtgatgaaa
    74761 ctgttgctga tcaagcgggt tttctgataa acgatcaagg agttcagtat aattttcttt
    74821 tcagcagcct tgtacaagat atccaaggaa cgatgagttt tgagattgcc aatcaattat
    74881 tcgaataagg agggatcagt gtatggaata ccacgaacta aatcccttga ttcgcggaag
    74941 agagcttgaa cttttgtcca atgagacatt tgaacggatg attcaaacaa attctgtcga
    75001 agcacttggt gaaatattga aatccacgat ttacagtcca tatatttatg acggatatga
    75061 atatgacttc gaacagaatc ttttaaagga gcagagccgt ctatttcgct ggttaaagga
    75121 atcagcaccg gagcctgaaa tcgtttggat ctatacgatg cgttatactt tccataatct
    75181 gaaggttttg acaaaagctg agatgacaaa tcaaaatctg gaccatttgt atatcgacga
    75241 tggattttat tcattagaaa ccttgaagga tgccattcat acgcaagttt caaatgaact
    75301 tcctgaaagc attatggaca gtatacgaga agtacgtgat tattttgaag agtctaccat
    75361 tcttcaggga attgacgtga tttatgatcg acagtttttg acagagcagc gacggcttgc
    75421 cgataagcta ggatatcaag aattgctgac agaagtcatt gcttttattg acttgaccaa
    75481 tatcacaacg atggctcgag gaatattgca gaaaagaaca tatgggttca tgacgacggt
    75541 actttccagc tcgggaagta tcgaaaaaga agtatttttg aactttgtag ataaagatat
    75601 ggaagattac acgaattttc tattagagac agattatgct gattttctgc gccctgtcat
    75661 ccgtgaggga aatattgatc tcgtaaagct tgaacagttg aaagatgatt atctaagttc
    75721 attgtatcaa gaagcacaaa cccaggcttt cggaccactg ccactactag cctttttgaa
    75781 cgcaaaagag gtagaaagca aaaaccttcg tttactggtt atcggcaaaa gaagccattt
    75841 cgaatcagaa acaatcagaa agcgggtgag acaagtctat gacgcataag atcggtgtag
    75901 ttggcgacaa agattctgtt ttgccattca aattgtttgg ctttgatgtt cgttatggta
    75961 caaccaagca aaccgttcgt caggcaattg aagaaatggc aaagaatgag tatggcgtga
    76021 tttatgtcac agagcaatgt gcagcattga gcccagaaac gatcgaacgt tacaaggaac
    76081 aaatgatacc tgctatcgta ttgattccca atcatcaagg atcattaggg atagggctgg
    76141 aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa tatcttatag tatgaaaagg
    76201 agatagaaat tttgcaaatt ggaaaaatca taaaagtctc cggacctctt gtaatggctg
    76261 agaacatgtc tgatgccagt atccaagaca tgtgtctagt gggagatcta ggagttatcg
    76321 gagaaattat cgagatgcgt ggcgatgtgg cgtctattca agtatatgaa gagacttctg
    76381 gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc tttatctgtt gaattaggac
    76441 cagggatcat ttcacaaatg tttgacggaa tccaacggcc attagatacc tttatggaaa
    76501 tcacacaaag taatttctta gggcgcggag ttcaattgcc cgcattggac catgagaaaa
    76561 aatggtggtt cgaaccaaca gtagaagccg gagaaacggt gagtgctgga gatgttatcg
    76621 ggatcgtaga agaaacaaaa gttataaaac ataaaatcat ggtgccaaat gggataaaag
    76681 gaacgatcaa aacaatcgaa aaaggttcat tcactattga tgaaacagta tgtgtcattg
    76741 aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa atggccagta agacgtagtc
    76801 ggccaatcaa agaaaaatta aacccggatg cacccatgat cactggacag cgagtgattg
    76861 atacattctt cccagtgaca aaaggaggag cagcagctgt acctggtcct tttggtgcag
    76921 ggaaaacagt cgttcagcat caaattgcaa aatgggccga tgtggacatg gttgtctatg
    76981 tgggatgtgg cgagcgtgga aatgagatga cggatgtttt aaatgaattc cctgaattga
    77041 ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt attgattgct aatacatcaa
    77101 acatgccagt agcagctcga gaagcttcta tctatacggg aatcacaatt gcggaatatt
    77161 tccgtgacat gggttatgac gttgctatca tggcagactc cacttcacga tgggcagaag
    77221 cacttcgtga gatgagcgga cgtttggaag aaatgcctgg tgatgaaggt tatccagctt
    77281 atcttggttc acggcttgcg gaatactatg aacgatctgg aagagtcatt gcattaggat
    77341 cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt atccccgtct ggaggggata
    77401 tttcggaacc agtcacacaa aatacattgc gggtcgtaaa agtattctgg ggtcttgatt
    77461 ccagtttagc ccaaaaaaga cattttcctt cgatcgattg gatacaaagc tattctcttt
    77521 attcaacaga agtagggaag tatatggatc atatccttca agctgactgg gcatccatgg
    77581 tcaccgaggg catgcgtatt ttgcaggaag aacagcagct gaatgaaatc gtgcgtttgg
    77641 tagggattga ttccctttca gataatgacc gtctgacatt agaagtggca aaatccattc
    77701 gtgaagatta tttgcagcaa aatgcttttg atgatgtcga tacattcact tcaagagaga
    77761 aacaattcaa aatgttgaag atgatcttga cctttggtga agaaggacgc cgtgcgttgt
    77821 cgttaggtgc ttatttaagt gagatcatgg acggtaccgt atcgattcgt gaaaggatcg
    77881 gacgaagcaa atatatcccg gaaacagaat tagaaaaact agatcaaatt attggtgaaa
    77941 tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat gagcaatgat taaagaatat
    78001 cgtacgatcg gtgaagtcgt cggtccattg atggcggttg aaaaagttgc tggtgtcaaa
    78061 tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga ttcgtcgtgg acaagtattg
    78121 gaagtccaag aagataaagc aatggtccaa atattcgaag gaacaagtgg tatcaattta
    78181 cgtgactctt ctgtacgatt tttaggccat cctttagaac taggtgtatc ggaagacatg
    78241 atcggtcgag tattcgatgg tttgggacga ccgaaagata acggaccaga gatcctgcct
    78301 gaaaagcatt tggatattaa cggagaagta atcaatccag tagctcggga ttatccagat
    78361 gagtttatcc aaacaggtat ctctgcaatc gatcatttga atacattagt tcgtgggcaa
    78421 aaattaccag tattttctgg ctctggtctt cctcataaag aattagctgc tcagatcgcg
    78481 cgacaagcaa cggtactcga ttcatcagat gatttcgccg tagtctttgc tgctattgga
    78541 atcacttttg aagaagctga atttttcatg gaagattttc gtcaaacggg cgcaatcgac
    78601 cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa tcgaacgtat cgctactcct
    78661 cgtatggctt tgactgctgc agaatatctt gcttatgaaa agggaatgca tgttttggtt
    78721 atcatgacag acatgaccaa ctatgcagag gcattgcgag agatttctgc agcacggcga
    78781 gaagttccag gaagaagagg atatcctggg tatctttaca cgaacttagc tactttattc
    78841 gaacgagcag gaagaatccg cggactaaaa ggatcggtta ctcaaattcc gattctaaca
    78901 atgccagaag acgataaaac tcatccaatc cctgacttga ctggttatat tacagaagga
    78961 cagatcatct tgactagaga gctttacaag agtgggattc agccaccaat tgatgtactc
    79021 ccgtctcttt cgcggttaaa agataaggga actggacccg gaaagacaag agaagaccat
    79081 gcagctacga tgaatcagct ttttgcagcc tatgcacaag gaaaacaggc aaaagaatta
    79141 gccgtagttt taggtgaatc tgctttatct gatgtagaca agatctatgc gaagtttgct
    79201 gagcggtttg aaaaagaata tgtcaatcaa ggattctaca cgaaccgttc gattactgaa
    79261 acacttgatc tagggtggga actgctatcg atgttgccaa gaaccgaatt aaaacgaatc
    79321 aaagacgaca tgctggacaa atatctgcca gaagggaagt gagttctgat ggcacgattg
    79381 aatgtgaatc cgacccgcat ggaattgact cgattgaaaa aacagttgac aactgccaca
    79441 agagggcata aattattaaa agataagcaa gatgaactga tgcgtcaatt catcctgttg
    79501 attcgaaaga acaaccagct gcgtcaagaa atggaagagt ctatgacaga agcaatgtct
    79561 gattttgtat tggcaaatgc ttcgatcacg gaacctttca tcgaagaatt atttgttttg
    79621 ccggcggaaa acgttgattt gtctatcgta gacaaaaata tcatgagtgt aaaagtacct
    79681 gtgatgaatt ttgaatatga tgaacagctg acagacacac ctttggaata cgggtatttg
    79741 aattccagtg ccgaactgga tcggtcaatc gatcggttta ctaccctgct tccaaaactt
    79801 ttggaactga cagaaatcga aaaaacttgt caactgatgg cgagtgaaat cgaaaagaca
    79861 cgtcggcgag tcaatgcatt agaatatatg acgatccctc aattagaaga aacgatctat
    79921 tatattcgta tgaagctgga agaaaacgag cgcgcagaag taacaaggtt gatcaaagta
    79981 aaaaatatgg gtaccactga ctaagtggta tcttttttta aaagaggaaa acgatgatta
    80041 aacaaagcaa acaaataaag aaacgtttgc cggctgttca gcttctcgca ttaggatttt
    80101 ttctcctgat tttagttggc ggcggcctac ttaccctacc attttttagt aatagtgggc
    80161 atgggacaaa ttttatcgat gcgcttttca ctgcaacttc tgctgtctgt gtaacgggat
    80221 tgacaacatt gaatacagca gagcattgga acagtgctgg tcaatttttg atcatgcttt
    80281 tgattgagat cggcggtctt ggttttatga tggttccgat tctatgcttt gcattgataa
    80341 agaagaaagt cagtctcagc acacgtatta tattaaaaga agcgctgaat ctagaagaag
    80401 tatcaggtgt gatgaagctc atgctttaca tcctaaaatt ggcctttgtg atccaattga
    80461 ttggtacggt tgctttatct tttgtatttg tgcctgaatt tggctggttg aaaggtatct
    80521 ggtacagttt gttccatgca atctctagtt tttgtaatgc agggtttgat ttgttaggag
    80581 acagtttagc caatcaccag caaaatgttt atttgatcat ggtggtatct gctttgatca
    80641 tttctggtgg gttaggcttc atcgtatggc gtgatttact ggaatatcat cagaaaaaga
    80701 agatgacgat tcattcaaaa atcgcattaa ccgttacagg taccctttta ttaggcggat
    80761 tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct agtggaaggg aatatgtttc
    80821 aacgattggc aaacaccttt tttatgagtg tgacacctag aacggctgga tattattcag
    80881 ttgattacct ccatatgagc catgcaggtc tgattttaac gatgtttttg atgtacatcg
    80941 gcggaacatc tgggtcgacg gccggcggat tgaaaacaac tacgttaggt gtgttgctga
    81001 tccagatgcg ttcgatgttg aaaggacgta cgagagcgga agcatttggc cgtaccatcc
    81061 gacaaggcgc agtgttgcgg gcacttactt tattttttgt tacgttgacg ttgtgtgtag
    81121 tcgccatcat gattttatcg gtgactgaaa cgatacctaa aacttctggc atcgaatata
    81181 ttgcttttga ggtcttctct gcgtttggta cagtaggtct tacgatggga ttgacgccag
    81241 acttgactgc aatagggaaa ttgatcatta tagcgcttat gtacattgga cgagttggtg
    81301 tattgactgt ggtcttctct ctgatgacaa aagcacaaag aacacaatca aaatacaaat
    81361 atccagaaga aagtgtcatg attggataat ttcatcttct gacagaaacc tagtgattta
    81421 gtaaaataag aaaataaaat aatttttctt gcttcttgga tactttaagt gcaaaatgtt
    81481 cgtttatttt ttgaggcact acccgttgtt gaacctcggg tagtgcctct tttttattga
    81541 aaggaatcaa taagatttga ttcctttatt tatcaatagg ttataattca tattttttta
    81601 ttttttcttt tacgatactt agtccagaag ctaaaatttc aaggttatta gaggataggg
    81661 aaattcgaac gaatgcatct ggttgcgtac gtgtagtaaa gcgtctggaa tgataaagtc
    81721 ggataccgtt agcaagaaag tcattctcaa ttatcttagg gtcaatctca ggatcaactg
    81781 gaagaattcg atagtaaggg acacgattcg ttggtttttc tactttgaaa agagaatcaa
    81841 aaagctgatt ggctcttttt gtaagactta atttagtttc taagatccta tttgcttcat
    81901 ttgaatggag gatttgagtg atgacttcca catctaatcc agaagttttc acatttatat
    81961 taaaaattgc ttgttctatc ttgctttgga catgtactgg aactacaaga taagcaactc
    82021 gcaatccaga acaaataaat tttgtcattc cagctaagta gattgtttgg ttaggtaata
    82081 aattttggaa aggaggaatg atcgtatgtt gatagtaagt tgttaaaaat gaatggatat
    82141 catcttcaat aacccataga ccttcttttt caatcacttc ggctaattgc tctcgtcttt
    82201 tgtgagagat ttgaaaccct ataggattat tacaagaagg cataagaaag acaccatgaa
    82261 tcttcttttt tctacattcc tgaatcaata tatcaggtcg cattccttgc tcgtcaaaat
    82321 caattggaac gatctctaaa tgatataact gagccaattc aatgaaatta gcgtatgtat
    82381 atcggtctgt tgctattcta tcacctggag agaaaagtgc agaaagtgtg atagccaatc
    82441 cattttgaac accggaaaca atagatagag attctaagga cggaatgact ccttgagttt
    82501 ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc tttgggatct tggtaattaa
    82561 gcaaaccaac aagatttggg ttattactag ttgattgaat aaacgggaca acgaatttat
    82621 tacattcttc aaaagaacta accagaccaa aatcaatgat atgttcggtt acttcattgg
    82681 tagagatggt gatggactca aagacattgg gagaaataaa tgtgccactt ccaatattcg
    82741 tatacagcaa acctttctca attccatatt tataagcctt gccaacggta gtaaaattta
    82801 aatctaaata atcagctagc tctctttggg agggtaactg agtgtttttc tgtaatttat
    82861 tctttaaaat atcttgttct aattgttgaa tcaacgatcg ataataagga cgcttcaatt
    82921 tttgtttgtc tggtcgccaa gttaacgggt aattgtcata agaattaata ggcattttct
    82981 ccactcctta aaattgtata ccatacaatt ttaccattga ttgtattgat tttagaagtt
    83041 aatcttttgt gaggagggaa ttgtctatga aaattattaa ttttgaggaa gtagtagctt
    83101 cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg tttttttgat tatgaaagtg
    83161 gtaaaatcag ccaaaatcct cgattggtgg aaattttacc tgacggaaaa ggcaaaaata
    83221 tgtttgcgat gatgcctgct tatttggggg agaacagata ttttggcaca aaagtcatta
    83281 ccgcttttcc ggataatcat cagtatcatt taccttctca tttaggagag attttcttgt
    83341 ttgatgctca aaacggacta cccaaagcat tgataaacgc aaatgcagtc acctggattc
    83401 gaacagctgc tgtatctgcg ctggcaacag attacttagc aaatccgcat gcagaaacac
    83461 ttgctttaat tggagctggt caacaagcat tgtcgcattt gactgcgatt tctaccattc
    83521 gtcccatcac tacagtgttt gtttacgata ttttagaaga aagacgaaat acttttatcg
    83581 ctaaagctaa aaaacagtat cctaaaataa cgtttataaa tgcaatatca gtagaagatg
    83641 cagtacgtcc agcggaaatc atttgtacgt tgacctctag taaagaggca tttcttcaaa
    83701 aagagtgggt aagggaagac gcacatatca atgctgtggg cacatttact cctgatacaa
    83761 gagagataag tacatcgttg atggaaactt ctcaaatcta tgtagatgat tactcagctg
    83821 ccttaacaga aagcggggac ctgcttatag ccatggcaga aggagattta gaggaatctt
    83881 gtattgttgg tacattaggc gaattagttt ctgagaaaaa aacattttat aaaggagaat
    83941 cgaggccgac gattttcgac gcagttggtc tagctattga agatctttgt tgtgcagaat
    84001 atatttataa caaattagag aaaggggctt taaaatgaac ttagattatt acgtagttga
    84061 tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg gtttatgtgc tagaagaatg
    84121 gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac aatttatctg aaacagcatt
    84181 tacagtgaag aaaaaccaag aatttgagct tagatggttt acgccagatc gagaaatcga
    84241 tctttgtggt cacgcgacat tagcaacagc atttgtttta tttaactatt acaaaatacc
    84301 tgatgaaacg attaaatttt ctactcaaag cggtaatcta tttgtgacaa gaattaaaga
    84361 ttattattac atggattttc ctagtattat gccgaaaaaa gtgccgatat tgtctgaata
    84421 tgaagaagcg attggggcta agattaaaga agcctactta gcacgtgatt tatttttcgt
    84481 attagaagat gaaaaaacgg tggctaaatt gcagccagat tttacagcta tcaaaaattt
    84541 tgaattagga ataggagtca tcgtgacagc agaaagtgtg caaaaagact ttgtttctag
    84601 aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag cacattctaa
    84661 tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc
    84721 atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa
    84781 agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg aattaaaaat
    84841 aaattggtgg agagtggcaa ccttatagct acaatggttg ttaaaaaaag acgcaaactt
    84901 tttcaattta atgaaaagtt tgcgtctcat tttaattatt ggtgtttgtt ttttattcaa
    84961 tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta taaccatcag
    85021 ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa tccttttaag tcagtaatct
    85081 caagaccagc agctttatgt tgctcgtgta atttttctag atcatcagct gctatagcga
    85141 tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa gtcaactcta
    85201 attcatagtc atccccagga agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat
    85261 cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt gatgcttcaa
    85321 gatctttcac tcgaacacat gtgtgagcca ttttcataga ttcatctctc cttttccttt
    85381 tactcaatca taacaaaaag aagtaaggaa atagttgatt tgtgcttgtt catgagaaat
    85441 ttctcatgta aaatggatag aaaataaaac tgttaaggtg gaaactattc atggataaac
    85501 caatttttgg tgaaaaaaag caatttgctg attatcaaac ccgttatgca gcatatattg
    85561 tcatagaaag agatcagcag attgcagtaa tcgaggctcc aaatggtgct ttttttcttc
    85621 ctggcgggga aattgaagga gaagagacga aagaagaagc gattgtccgt gagctgatag
    85681 aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat gagtattttt
    85741 actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt gctgattctt
    85801 ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg agccctcaag
    85861 aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga tggatcgaaa
    85921 aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt tatttattac
    85981 agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca
    86041 tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag accagcatat
    86101 tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc ttgatcaata
    86161 ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt atttagtact
    86221 tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa
    86281 cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt gacgatgttc
    86341 atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac taaacttgct
    86401 tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc
    86461 gaatggagat gactgccgac acggcgtgcc atcacttcga tctctaggaa atcttcatgt
    86521 gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag tgccccgtta
    86581 taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg ttcctggaca
    86641 cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac agcagcggca
    86701 atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt accatcgatg
    86761 tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt tatttcaatc
    86821 aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa taaatcataa
    86881 tgatcacgca aggacggatc aaccatttct tttggaaaga aaaatcgctc atctccttgt
    86941 tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat caaagttccg
    87001 tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca
    87061 taaggtagat cgtagctaga attcacagca gtgtaataaa caggattata tccgaccttt
    87121 tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga aaaagtagca
    87181 gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat aggatcagca
    87241 gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt gaggtattct
    87301 tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag ttcaaaactg
    87361 ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa aattacttcc
    87421 ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg gctaacgata
    87481 taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc ataaggacga
    87541 atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt gaaataagca
    87601 tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat ttggacaact
    87661 tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa accttcttct
    87721 actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc tacagtgatg
    87781 gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc atgaccgaca
    87841 tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca gcctccgttg
    87901 cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct tgaaatctca
    87961 taaacgccta atgaatgagt aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa
    88021 gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt tcgaattgat caaatctaag
    88081 attacttgat gctggacatg gatatagtta tggactggat cacgaaaaac tttttccata
    88141 ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt tattacgttg
    88201 ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt ttagccagtc
    88261 ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg caaatgcttc
    88321 tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg ccattgtagc
    88381 aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag attgataaaa
    88441 tcgcttcatg acttgcccgc gtttttcctg attaccgttg acacttaagt acatcataac
    88501 agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc catggatgct
    88561 taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta tcggtgcctt
    88621 tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta tggttatttt
    88681 ttttgtgtca ctttggggga aaatcaaaga aatattcaaa attccttcat ctgatacgat
    88741 ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatctttca gtgtttggat
    88801 cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg tgcagtcata
    88861 ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta atatatctgt
    88921 taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg
    88981 gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat actctaaaat
    89041 caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt tgacaaaaca
    89101 aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat ggaattatca
    89161 caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa
    89221 gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct gtacatacgt
    89281 tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa attagttcca
    89341 gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat
    89401 aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat tgctactttt
    89461 acgaaagaac tgcatgttag tttgaaagac cgtcccacag cagggaaggt tcgaatcgat
    89521 tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt ggattttaca
    89581 gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat aaggagtaga
    89641 ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa
    89701 ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt catggatttt
    89761 tctgatttag ctaaccagat ccaaaactat cttgggaaat cagacagtga aattcgtgat
    89821 tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc attaggggat
    89881 aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga agtaaaccat
    89941 ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa agtcaacgcc
    90001 tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga agatacagat
    90061 ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga tgaagaaatc
    90121 gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga tgatgaagaa
    90181 gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga tgatgatgaa
    90241 gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt ttcttacgag
    90301 aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacga tttttgtcac
    90361 accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa aaattgaaac
    90421 ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga atgattttta
    90481 ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat ggactcatcg
    90541 aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat aaagaagagg
    90601 ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa aaagaaaaag
    90661 agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca ttagatagtt
    90721 ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc ccccaacagt
    90781 tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata gacagtccgg
    90841 aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa attgcgacta
    90901 tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg aatgctttac
    90961 gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga aaatttaaaa
    91021 aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga ggagatccgc
    91081 cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa gagacgtctc
    91141 caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct tctttttaat
    91201 agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat tgaattgatc
    91261 ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc gaagatcaat
    91321 acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata ctttcgcgaa
    91381 ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac ggatcattac
    91441 caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt gattgaaagg
    91501 cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac aatcgacaag
    91561 aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta tctatctgtt
    91621 ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc atctaagtac
    91681 tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact tgttacgcct
    91741 acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc tcaatacgaa
    91801 attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt gacaaaaacg
    91861 attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga ctagcagttt
    91921 caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga agagatatag
    91981 tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg tttttcgaaa
    92041 ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct tcggagcatt
    92101 cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca actgaaacag
    92161 ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag tcatcacaat
    92221 caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc gcaatcagct
    92281 ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta aaagaaaaaa
    92341 acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct gaaatcgtga
    92401 agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat cagaatctgg
    92461 ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact gagatcaaaa
    92521 aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc acgaatgtag
    92581 gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa gattggaaga
    92641 caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat ggggcaatta
    92701 ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg atcgattatc
    92761 tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat ccaacggctg
    92821 tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga caagtatctt
    92881 taataaataa cacattttaa taagtagttg aacacataac taccttactt gcaaaggctt
    92941 aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg cggacagttt
    93001 tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag tagaacgcgt
    93061 cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc cactacacta
    93121 aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag gggattggac
    93181 aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca gaaactagcc
    93241 atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga ggagctgttg
    93301 gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga gccggaatgg
    93361 cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac aaattaccta
    93421 aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca agtccatttg
    93481 aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata catcgatttg
    93541 aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc tacgcctatc
    93601 caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt aggtgcacgt
    93661 agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc ggaattagct
    93721 tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa agaaatcgca
    93781 caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt acaaacgttg
    93841 agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat tcctgatgtc
    93901 cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa aaatcagcca
    93961 aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga taaagaaact
    94021 gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac gatcctacaa
    94081 cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa agctttcttt
    94141 atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac tgagatagat
    94201 gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac agacttcggt
    94261 gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac agaacagaca
    94321 gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga tcaagaagac
    94381 aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga tgagatagcg
    94441 atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag ttcatcaaaa
    94501 agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca ctttttctag
    94561 tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag gttgagattt
    94621 ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata tgggtcgttt
    94681 taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg atgaaataaa
    94741 aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct ataaaatttt
    94801 acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa gtatggggat
    94861 cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt tgatcgacac
    94921 ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa gccttgaaaa
    94981 cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag aatattttgg
    95041 taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag tggctgatgc
    95101 attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg agaatatcat
    95161 cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta atctggaaaa
    95221 taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag cgataactgt
    95281 acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc accgtgcact
    95341 ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg agccatctat
    95401 ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta ttgggcgtgc
    95461 attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc tgaaacaagc
    95521 aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag tcgtttataa
    95581 ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca tcatggcaat
    95641 cgcgggcgga aaatcaaaag ctaaagcaat tcgagcttat atgaaaaatg caccaaaaca
    95701 aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg taacccttta
    95761 aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt aaagtaggta
    95821 ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat gtagatggaa
    95881 tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac ttgttaaaat
    95941 acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt tcattcaacg
    96001 taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta ccatggggcg
    96061 aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa tcagcagctg
    96121 aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc ggaaacgatg
    96181 ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa acagttatct
    96241 caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg aacgacaaat
    96301 ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac caaatgactc
    96361 tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct gcaaacatcg
    96421 ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa ttgaacggta
    96481 aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca gaattagtaa
    96541 cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa gaagcttcaa
    96601 acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt ggtatgactt
    96661 acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac aaacaattag
    96721 ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta gtacgtactt
    96781 tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta tagacatgta
    96841 catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata agtttattca
    96901 ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga caaaaaagtt
    96961 cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga cgacactcgt
    97021 atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa agcaatcctt
    97081 ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc tttagctcca
    97141 gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc tgaaactcgc
    97201 ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt cgtattcgaa
    97261 aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac agaactaggt
    97321 aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac agctcaccgt
    97381 gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc tggattcttg
    97441 atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg accaatgatt
    97501 gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa cttgattcct
    97561 aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga agcaaaagga
    97621 atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa agaattaatc
    97681 gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc agaattttca
    97741 aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat ggctttagac
    97801 atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa aacagttgtt
    97861 tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac aatcggtgtt
    97921 tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg agactcagcc
    97981 gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac aggtggcggc
    98041 gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat caacgataaa
    98101 taaacgacca agtttgctca attctgacga gtaatcgaac aaaaggtgaa gggagttttc
    98161 atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc gtaaacctat
    98221 tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat ttgctgaagc
    98281 agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat ctcctgcatt
    98341 attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct cagcacaaaa
    98401 ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag ctcttgctga
    98461 tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt tccatgaaac
    98521 agacgaagac atcaataaaa aagcgaaagc tatctttgca aatggtatga caccaatcct
    98581 atgctgtggc gaatctttag aaacctatga agcaggcaaa actgctgaat ggatcgaagg
    98641 acaaattaca gctggtttag ctgatctttc agctgaacag gtgtcaaaca tggttattgc
    98701 ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa tcgctgacga
    98761 aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag tatcagaagc
    98821 agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat acatggcaaa
    98881 agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt cattcttgtc
    98941 attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga aatgtttgca
    99001 ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa aaaacaaact
    99061 caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag atcttagact
    99121 cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct tttggccgcg
    99181 gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta cgtgacggag
    99241 acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta aacaacatca
    99301 ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac aaagctatga
    99361 tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt ttaggtgttt
    99421 ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac cactacctag
    99481 gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac ggcggatcac
    99541 atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc gctccaacat
    99601 tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca atcttgaaag
    99661 ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac cttggttcaa
    99721 acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat gtacctggta
    99781 aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa gaaaaaggtg
    99841 tttacgtact agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg atcaaattct
    99901 acgaagaatt agtttctaaa tatccaatca tctcaatcga agacggatta gacgaaaacg
    99961 actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa ttagttggtg
   100021 acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa ggaattgcta
   100081 actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc gaagctatcg
   100141 aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt gaaacagaag
   100201 attcaacaat ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa actggttctc
   100261 tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa gaccaacttg
   100321 gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac aaataattct
   100381 tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc acttagttaa
   100441 gtaatcgaac tctctacttg ctgaaatatc agcaggtaga gagttttttt gcaactagct
   100501 tctgtatatg atgtttacaa agaatggtac aagaaaagat catccttgaa aaaggaaagc
   100561 taacaataac tagcatcata taagagcttt taaggataga acagtagaat aggaatctat
   100621 gagcaagtcc tctcgtataa ataaaatagg agggaagtag cagcatattt ttcataaagt
   100681 ggctctcttt cttcattttt actttggttg ctcctatttg aaaattcgag cacactcaaa
   100741 tacaataaaa agaaatggca agggagagga ttagatgaaa aaagaagaaa aaatcgcgat
   100801 tttacaagag atcatccgaa ttaaatcagt gaatggaaac gaaggtgaag tagcagccta
   100861 tttgaataag cttttggcaa agcatgacat aacaggagag attgtttctt acagagatgg
   100921 tagagataat ttgattgctc gttaccaaaa gggccaatca ggaaaagtgt taggcctatc
   100981 tgggcatatg gatgtcgtgg cggctggtga tgaatcgtct tggacctatg caccctttgc
   101041 agcagaaatc catgggaacc gtttgtacgg acgtggtgct acagatatga agagtggctt
   101101 ggcagcaatg gtgattgcca tgatcgaact gaaagagtca ggaaaaccat ttaacggaac
   101161 agtcaaatta ttagccactg tcggagaaga agtgggtgaa ctaggtgggg aacagctcac
   101221 gaaagcaggc tatgtagatg acttagatgc cttgatcatc ggagagccaa ccaattacag
   101281 tttgatgtat acgcacatgg gatcaatcaa ctatacagtc acctcgcacg gaaaagaagc
   101341 gcatagctct atgccagacc aaggttataa tgcaatcaat cacctaaatg aattcattac
   101401 gaaagcaaat gctgaaatga atcacctcgc tgaaacaatc gagaatcctg tcctaggaaa
   101461 aacaatccat aatgttacat tgatcagtgg cgggaatcaa gtcaacagca tcccaagcca
   101521 tgcgcagctc caagggaaca ttcgatcgat tccagaatac ccaaatgata aaatcattgc
   101581 attgctgcaa tcaatcgtca atgaattgaa tcaagagaca gactatcatt tggaattgat
   101641 gatcgattac aacaaaatac ctgtcaaagc tgatccagat tctcctttga ttcattgcat
   101701 tcagcaacaa ttcagccagc cattaccttt agttggagcg gcagcaacaa cggatgcagc
   101761 cgaatttacg aaagctaacc attccttcga ctttgtcgtc tttgggcctg gtgtcgttac
   101821 tttgcctcat caagtagatg agtatgttga aatcgataat tatttggata tgatcgaaaa
   101881 atatcaagga attattttat cctacttagc gtaagatttt tttaataaat gaaaaaacag
   101941 agccagatta gcatta
//
LOCUS       DAFCNS010000002        87683 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFCNS010000002 DAFCNS010000000
VERSION     DAFCNS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.002"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(11..616)
                     /gene="dhaL"
                     /locus_tag="LNE23_000104"
     CDS             complement(11..616)
                     /gene="dhaL"
                     /locus_tag="LNE23_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBK6929998.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(644..1654)
                     /gene="dhaK"
                     /locus_tag="LNE23_000105"
     CDS             complement(644..1654)
                     /gene="dhaK"
                     /locus_tag="LNE23_000105"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBK6929999.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1629..2075)
                     /gene="dhaM"
                     /locus_tag="LNE23_000106"
     CDS             complement(1629..2075)
                     /gene="dhaM"
                     /locus_tag="LNE23_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBK6930000.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2178..2498
                     /locus_tag="LNE23_000107"
     CDS             2178..2498
                     /locus_tag="LNE23_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBK6930001.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2610..3479)
                     /locus_tag="LNE23_000108"
     CDS             complement(2610..3479)
                     /locus_tag="LNE23_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6930002.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3575..4879)
                     /locus_tag="LNE23_000109"
     CDS             complement(3575..4879)
                     /locus_tag="LNE23_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6930003.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4876..5385)
                     /locus_tag="LNE23_000110"
     CDS             complement(4876..5385)
                     /locus_tag="LNE23_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930004.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5560..7695)
                     /locus_tag="LNE23_000111"
     CDS             complement(5560..7695)
                     /locus_tag="LNE23_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6930005.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7778..9433)
                     /locus_tag="LNE23_000112"
     CDS             complement(7778..9433)
                     /locus_tag="LNE23_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6930006.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9435..9749)
                     /locus_tag="LNE23_000113"
     CDS             complement(9435..9749)
                     /locus_tag="LNE23_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK6930007.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9919..10179
                     /locus_tag="LNE23_000114"
     CDS             9919..10179
                     /locus_tag="LNE23_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBK6930008.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10193..10507
                     /locus_tag="LNE23_000115"
     CDS             10193..10507
                     /locus_tag="LNE23_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBK6930009.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11237..11791)
                     /locus_tag="LNE23_000116"
     CDS             complement(11237..11791)
                     /locus_tag="LNE23_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBK6930010.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11917..13846)
                     /locus_tag="LNE23_000117"
                     /pseudo
     CDS             complement(11917..13846)
                     /locus_tag="LNE23_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14152..14868)
                     /locus_tag="LNE23_000118"
     CDS             complement(14152..14868)
                     /locus_tag="LNE23_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK6930011.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14869..16692)
                     /gene="glpO"
                     /locus_tag="LNE23_000119"
     CDS             complement(14869..16692)
                     /gene="glpO"
                     /locus_tag="LNE23_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBK6930012.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16706..18202)
                     /gene="glpK"
                     /locus_tag="LNE23_000120"
     CDS             complement(16706..18202)
                     /gene="glpK"
                     /locus_tag="LNE23_000120"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBK6930013.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18338..19798)
                     /locus_tag="LNE23_000121"
     CDS             complement(18338..19798)
                     /locus_tag="LNE23_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930014.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19788..21083)
                     /locus_tag="LNE23_000122"
     CDS             complement(19788..21083)
                     /locus_tag="LNE23_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6930015.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21415..22092)
                     /locus_tag="LNE23_000123"
     CDS             complement(21415..22092)
                     /locus_tag="LNE23_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBK6930016.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22122..23213)
                     /locus_tag="LNE23_000124"
     CDS             complement(22122..23213)
                     /locus_tag="LNE23_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBK6930017.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(23314..23748)
                     /locus_tag="LNE23_000125"
     CDS             complement(23314..23748)
                     /locus_tag="LNE23_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6930018.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24332..25789)
                     /gene="ascB"
                     /locus_tag="LNE23_000126"
     CDS             complement(24332..25789)
                     /gene="ascB"
                     /locus_tag="LNE23_000126"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK6930019.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25786..27681)
                     /locus_tag="LNE23_000127"
     CDS             complement(25786..27681)
                     /locus_tag="LNE23_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6930020.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27739..29514)
                     /locus_tag="LNE23_000128"
     CDS             complement(27739..29514)
                     /locus_tag="LNE23_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK6930021.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29564..31060)
                     /locus_tag="LNE23_000129"
     CDS             complement(29564..31060)
                     /locus_tag="LNE23_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6930022.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31254..32876)
                     /locus_tag="LNE23_000130"
     CDS             complement(31254..32876)
                     /locus_tag="LNE23_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK6930023.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33204..34517)
                     /locus_tag="LNE23_000131"
     CDS             complement(33204..34517)
                     /locus_tag="LNE23_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK6930024.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34798..34986)
                     /locus_tag="LNE23_000132"
     CDS             complement(34798..34986)
                     /locus_tag="LNE23_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930025.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKE"
     gene            complement(35006..35428)
                     /locus_tag="LNE23_000133"
     CDS             complement(35006..35428)
                     /locus_tag="LNE23_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBK6930026.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35573..36283)
                     /locus_tag="LNE23_000134"
     CDS             complement(35573..36283)
                     /locus_tag="LNE23_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930027.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36301..36798)
                     /locus_tag="LNE23_000135"
     CDS             complement(36301..36798)
                     /locus_tag="LNE23_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6930028.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPKHLADYLTQAYAPEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36835..37287)
                     /locus_tag="LNE23_000136"
     CDS             complement(36835..37287)
                     /locus_tag="LNE23_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6930029.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37422..38027)
                     /locus_tag="LNE23_000137"
     CDS             complement(37422..38027)
                     /locus_tag="LNE23_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK6930030.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38582..39217)
                     /locus_tag="LNE23_000138"
     CDS             complement(38582..39217)
                     /locus_tag="LNE23_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK6930031.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKKSMTISNKEMA"
     gene            complement(39219..40088)
                     /locus_tag="LNE23_000139"
     CDS             complement(39219..40088)
                     /locus_tag="LNE23_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBK6930032.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYISMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(40111..40518)
                     /locus_tag="LNE23_000140"
     CDS             complement(40111..40518)
                     /locus_tag="LNE23_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK6930033.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELYENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRSD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40537..41709)
                     /locus_tag="LNE23_000141"
     CDS             complement(40537..41709)
                     /locus_tag="LNE23_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK6930034.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAILWMATMTTVFGY
                     FAGLRGIVLILVGGFIGACFAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVA
                     KLFGEKGENKKSIEDIKLPTHFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGD
                     QNYIMFAFLQAIQFVVGVYVLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFP
                     YSPNAVILGFITTTIGTIIAMFTLPMFGLAMILSGMLTNFFAGGTAGIFGNAVGGRRG
                     AIIGGIAHGFFITLLPALLVTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(41736..42038)
                     /locus_tag="LNE23_000142"
     CDS             complement(41736..42038)
                     /locus_tag="LNE23_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6930035.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(42063..44149)
                     /locus_tag="LNE23_000143"
                     /pseudo
     CDS             complement(42063..44149)
                     /locus_tag="LNE23_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            complement(44638..45403)
                     /locus_tag="LNE23_000144"
                     /pseudo
     CDS             complement(44638..45403)
                     /locus_tag="LNE23_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
     gene            complement(45514..46383)
                     /gene="mdcB"
                     /locus_tag="LNE23_000145"
                     /pseudo
     CDS             complement(45514..46383)
                     /gene="mdcB"
                     /locus_tag="LNE23_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
     gene            complement(46408..47492)
                     /locus_tag="LNE23_000146"
                     /pseudo
     CDS             complement(46408..47492)
                     /locus_tag="LNE23_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            complement(47516..48109)
                     /locus_tag="LNE23_000147"
     CDS             complement(47516..48109)
                     /locus_tag="LNE23_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBK6930036.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWATEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(48113..49789)
                     /gene="mdcD"
                     /locus_tag="LNE23_000148"
     CDS             complement(48113..49789)
                     /gene="mdcD"
                     /locus_tag="LNE23_000148"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBK6930037.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDFIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEETIVISIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKFRQAGYPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(49779..50078)
                     /locus_tag="LNE23_000149"
     CDS             complement(49779..50078)
                     /locus_tag="LNE23_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBK6930038.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(50180..51826)
                     /gene="mdcA"
                     /locus_tag="LNE23_000150"
     CDS             complement(50180..51826)
                     /gene="mdcA"
                     /locus_tag="LNE23_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBK6930039.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVVADKADKDGNL
                     YTGFNTEETPTIVEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADEPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALVVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLREAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(51833..52744)
                     /locus_tag="LNE23_000151"
     CDS             complement(51833..52744)
                     /locus_tag="LNE23_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK6930040.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLTFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEMVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            52864..53154
                     /locus_tag="LNE23_000152"
     CDS             52864..53154
                     /locus_tag="LNE23_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6930041.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLYTLEEGINYCLRSMRRSLLPSYCI"
     gene            53106..53720
                     /locus_tag="LNE23_000153"
     CDS             53106..53720
                     /locus_tag="LNE23_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBK6930042.1"
                     /translation="MSSVNEKIVTSVILYLEVASVSIPNLVRIFMEKHPDIQLTIMQH
                     HLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAIPKKHPLAKKEHLQLEDIQRTPIL
                     MLSKSNAFRHTLDAALEAKSIELTISSTTDDPATLRSIVKQGLAVSFFPKVSWSYDKN
                     DPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAKTMREFYLGK"
     gene            complement(53907..54269)
                     /locus_tag="LNE23_000154"
     CDS             complement(53907..54269)
                     /locus_tag="LNE23_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBK6930043.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            54615..56052
                     /locus_tag="LNE23_000155"
                     /pseudo
     CDS             54615..56052
                     /locus_tag="LNE23_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(56059..58611)
                     /locus_tag="LNE23_000156"
                     /pseudo
     CDS             complement(56059..58611)
                     /locus_tag="LNE23_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(58725..60056)
                     /locus_tag="LNE23_000157"
     CDS             complement(58725..60056)
                     /locus_tag="LNE23_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6930044.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYSLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELAAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQMIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCTHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(60022..61500)
                     /locus_tag="LNE23_000158"
     CDS             complement(60022..61500)
                     /locus_tag="LNE23_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6930045.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPKKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(61805..63640)
                     /gene="tdc"
                     /locus_tag="LNE23_000159"
     CDS             complement(61805..63640)
                     /gene="tdc"
                     /locus_tag="LNE23_000159"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK6930046.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTTHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHMTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
     gene            64109..65536
                     /locus_tag="LNE23_000160"
     CDS             64109..65536
                     /locus_tag="LNE23_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930047.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQVSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRLTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERTDRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(65628..65993)
                     /locus_tag="LNE23_000161"
     CDS             complement(65628..65993)
                     /locus_tag="LNE23_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK6930048.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQNDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(65990..66355)
                     /locus_tag="LNE23_000162"
     CDS             complement(65990..66355)
                     /locus_tag="LNE23_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK6930049.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            66793..66921
                     /locus_tag="LNE23_000163"
     CDS             66793..66921
                     /locus_tag="LNE23_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBK6930050.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            66978..68672
                     /locus_tag="LNE23_000164"
     CDS             66978..68672
                     /locus_tag="LNE23_000164"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBK6930051.1"
                     /translation="MRKSKSIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKSNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            68676..68795
                     /locus_tag="LNE23_000165"
                     /pseudo
     CDS             68676..68795
                     /locus_tag="LNE23_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            68799..68918
                     /locus_tag="LNE23_000166"
                     /pseudo
     CDS             68799..68918
                     /locus_tag="LNE23_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            69446..70579
                     /locus_tag="LNE23_000167"
     CDS             69446..70579
                     /locus_tag="LNE23_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBK6930052.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNVLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            70610..70900
                     /locus_tag="LNE23_000168"
     CDS             70610..70900
                     /locus_tag="LNE23_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBK6930053.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKQ"
     gene            complement(70960..71419)
                     /locus_tag="LNE23_000169"
                     /pseudo
     CDS             complement(70960..71419)
                     /locus_tag="LNE23_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(71549..72802)
                     /locus_tag="LNE23_000170"
     CDS             complement(71549..72802)
                     /locus_tag="LNE23_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6930054.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(72831..73148)
                     /locus_tag="LNE23_000171"
     CDS             complement(72831..73148)
                     /locus_tag="LNE23_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK6930055.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(73174..73494)
                     /locus_tag="LNE23_000172"
     CDS             complement(73174..73494)
                     /locus_tag="LNE23_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6930056.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(73959..75374)
                     /locus_tag="LNE23_000173"
     CDS             complement(73959..75374)
                     /locus_tag="LNE23_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6930057.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREHEERYMDEKGIVQDDYRIEF
                     IGQHLRYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(75390..76706)
                     /locus_tag="LNE23_000174"
     CDS             complement(75390..76706)
                     /locus_tag="LNE23_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6930058.1"
                     /translation="MRGSGMSFMDKLAKVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISYLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(76856..77554)
                     /locus_tag="LNE23_000175"
     CDS             complement(76856..77554)
                     /locus_tag="LNE23_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBK6930059.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(77843..78803)
                     /locus_tag="LNE23_000176"
                     /pseudo
     CDS             complement(77843..78803)
                     /locus_tag="LNE23_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(78917..79750)
                     /gene="msrA"
                     /locus_tag="LNE23_000177"
     CDS             complement(78917..79750)
                     /gene="msrA"
                     /locus_tag="LNE23_000177"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK6930060.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(79984..80727)
                     /gene="truA"
                     /locus_tag="LNE23_000178"
     CDS             complement(79984..80727)
                     /gene="truA"
                     /locus_tag="LNE23_000178"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK6930061.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(80918..81553)
                     /locus_tag="LNE23_000179"
     CDS             complement(80918..81553)
                     /locus_tag="LNE23_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBK6930062.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(81700..82941)
                     /gene="sstT"
                     /locus_tag="LNE23_000180"
     CDS             complement(81700..82941)
                     /gene="sstT"
                     /locus_tag="LNE23_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBK6930063.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            complement(83262..83939)
                     /locus_tag="LNE23_000181"
     CDS             complement(83262..83939)
                     /locus_tag="LNE23_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBK6930064.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKNVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(84190..84732)
                     /locus_tag="LNE23_000182"
     CDS             complement(84190..84732)
                     /locus_tag="LNE23_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK6930065.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(84737..84964)
                     /locus_tag="LNE23_000183"
     CDS             complement(84737..84964)
                     /locus_tag="LNE23_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK6930066.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(85043..86923)
                     /locus_tag="LNE23_000184"
     CDS             complement(85043..86923)
                     /locus_tag="LNE23_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK6930067.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTARVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            87209..87544
                     /locus_tag="LNE23_000185"
     CDS             87209..87544
                     /locus_tag="LNE23_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6930068.1"
                     /translation="MGIEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
BASE COUNT        25648 a        18555 c        14798 g        28682 t
ORIGIN      
        1 tttattgaac ttaaggttga ttgatcacat ctaaaagcgt tgaaaataaa atggcactag
       61 aagcagctcc tgggtcgata tgtccgattg cacgctctcc tagataggaa gctcgtcctt
      121 ttttagctac caagactttg gtgtgttctt ttgcctggtc aatgatttct tttgttaaat
      181 tccctgcctt caatgcctct gtgactggca gccaaacatc taacatcgtt ttatcgtcag
      241 cttctgcctt tcctcggctt tgaatacccg ctgttccctg ctcgatcact tcgcctaatt
      301 tttcttgaga atcaatagaa tcgacctctt ttgtcgcttt catcatgttc atgaatgcag
      361 atccgtataa cggaccagaa gccccaccga ctttcgatat catcgccata gagaatactt
      421 tgaatgtatc gctgattgtt tcaggatgat cggcttgaaa agcttcttca taagctgaaa
      481 cccctcttcc catgttattt ccatggtctc catcgccaat cggggtatct agatcactga
      541 gatattgttt gttctgattg attgcctctg caaattgcgt gagccattct tggatttctt
      601 gaacggttag tttcatatta tcaccctttt atcttgagat tttttaccat gaaatcgtct
      661 gtacattgct ttctaaagaa tctttccatt gtgtctccgt caagtcgatc agtgttagag
      721 aaagaccttg catatctagt gaggtcataa aattaccgac ttttcggaaa actacattga
      781 cgcctctttc ttccaacaga gctaaaacat cattcatgaa cacgaactgt tccatcaaag
      841 gcgtcccgcc cattccgttg actagtacac ctgcttcttt tggtaaattc gagtaatcat
      901 ctaaaatctt agtaacaagt tcctttgcca gtactttaga aggctgcatc ttttctcgac
      961 gatagcctgg ctcaccgtgg ataccaacac catactcgat ctcatcttct cctagttcga
     1021 atcctggttt ccctacttct ggtacagttg ctgctcgaag cgctagaccg atcgtcttag
     1081 ttgcctgtac gaccttttct cccagttctt tcaactcagc tagggaagca ccgtttcttg
     1141 ctgcatcgcc cacgattttg tgtacaagga cagtacctgc aactccgcgt tttccagcag
     1201 tatacgtact attttcaacg gcgatatcat catctacgat cacactttct acttcaatat
     1261 cgtccatagc agctaattcc tttgccatat cgaagttcaa tgcatcccct gtataattct
     1321 taacgatcag caaaactcct tttccactat cggcttcctt aatagccgtc tgaatctgat
     1381 ctggagttgg agaagtaaac acatcaccaa gcaccgctgc acttaacatc ccttccccta
     1441 caaacccggc atgggatggt tcatgtccgc ttcctccacc agaaaccaaa ccaacttgtt
     1501 ccgctttctg attcttggct acaacacggg aagattctac acgatgaaca agttctggat
     1561 agctttttac taatccttcc agcatttctt cgacaatatt gcttggatca ttcatgattt
     1621 ttttcaattt attcccatcc tttctttccg gcattttgtg cttctgttaa gatttgctga
     1681 gtatcatctg ttataccggc agtgatggct gctgcaaaag ctccttccac taaaggcgca
     1741 tccaccagat gataacgttg ctgctgctct tcttccaaca tgtcttttgc cagttccgag
     1801 ttcaataccg cgctaccgat atccgcaaaa acaaaataac tagctgcatc gcttgattca
     1861 ttgactgctt ctacgatttt catcgggtct gatccgatct cgccatttat tccgccgaga
     1921 gaaaaaattt gcacctcttc ggatttttgc atctgctcaa tcatttcttt aataccatct
     1981 gttacttttt gactatgaga aataagcaaa atactttttt tcacaagaat cttcctttct
     2041 tttacatagt tgctaacgat tggtttcgct ttcattaatg aacaattcaa gtatataata
     2101 aatttctaat tttgggaaaa tatgtgtagt gcttcttgaa gtttgttata ctttaattaa
     2161 aagagaggag tgatcgaatg attacaacaa caacgaacag tgtagaggga aaagccgtag
     2221 tagcttataa agggatcgtt tttggtgagg tcattactgg gatccatgca tttaaagact
     2281 tggaggcggg gttgcgaaat atctttggcg gccgctcaaa aagctatgaa aacgaactaa
     2341 tgggtgcaag agaagaagca cttgccgaaa tggccgaacg tgcaaaagca ttaggagctg
     2401 atgcgatcat tggcgtaaaa atggactatg aagtattagg cagcgacaat ggcatgctga
     2461 tggtcacatg tagtggaaca gctgtctcca cgagataagt aggggacatc tgttttaata
     2521 actaaagaga gatgggacat gtagtggcac tcaagaatta gatttttcag tctaacttcg
     2581 aggtactcta ccttcccatc ctctcttttt tatttctttt tgcggatgat tccgtaagct
     2641 gctgcgacta acacacccgc ccctgctaag gaataactga cagaagctgt tttttcattt
     2701 gtttttggca aagtctgact tgttgatttg gatggctctg tcgtttgctg aactgtttct
     2761 gtcgtgcttc cagaggtcac tgatttttct cctgccgaat gcaaaatatc ttttattcgt
     2821 tggaaactag ctgctaagtc agcttttttt gcttcaaaat cattagcaac tgtttgtgct
     2881 tcgctgatct ttccgtggat tgcttgactg gccgctgtga tatccgcttg agtttgactt
     2941 agctttccag aagctgtttg agcaacctga tttaattgct gattgaattc ttttgcttgt
     3001 tggatcttcc cgtcaagatc tgtttggaca gcttccgctt ttgctttcgc ttcggcaagc
     3061 tttttctcaa gttgtgcacg gtcatttccc actttatttt taagatcagc taaatcttga
     3121 tcgattgctg cttggtcttc tttcaatttg ttttttgcag cttctaatgc ttcttcgagt
     3181 tcttttcttt tattttccaa gtcttgcaaa tgatcgattt ggtcagaaga ggtagaacta
     3241 tttattgatg tatcttctga agatgaaatc gtttctcctg aattatctgt cggtacattg
     3301 gaagtcattt ctgtttccga ggcaccgttt tcagcaccag aagagcttat ggttgttgaa
     3361 ggttctgttt gagcaggttc agaagtttcc gcaaaagctg tctgtcccaa taagaaaata
     3421 ctggaaacag caattggcca gattaacggc ttcattcttt ttttcatcac ttttcccatc
     3481 cttactactt tttaactaat ttcttattat aagcaaaaat gtttggaaga accataaaca
     3541 caacaggctg tttgtctaaa aaacggtgca tcttttattt ttttgtacgt tccgggcttt
     3601 tccatgtccc gcttccataa cctaaaatcg ttttgactgc tggaatgaaa gtcgtcacga
     3661 tcgttatcgc gttcactatc cagaacaaaa gcatatatag tggtgcgaaa agaaggtatt
     3721 tcaattttct tcgtcgatca tctacaatca gcgaagctag caactggaaa aatcctgcga
     3781 ccatttcaaa gcagatgaac acaaaactga atgtaaaagt aatataaatc tgttcgtagt
     3841 ttccttgata tacataatgc ccgattaatc cagcaaaaag aactacggaa atacaaaaga
     3901 atattgacca gacaatactc aatgtttgat caatgaagat gatcgttctt ccaatatgtt
     3961 taaaaggatg gagcatcact tttttgaaat tcgtcagcca gacttctgtt ccaccttttg
     4021 cccaacgttt tctgtgtcga tatagcatct tcaacgtcac aggtacttcc atgaaaaaga
     4081 taatctcagg agcaaaaaca gataaccagt catttaactg atgatcccaa gcaatactga
     4141 tgtcttctgt cgctcgatct tgtctgaatc cgcccacatc gatcaaggct tcttttcgat
     4201 acatcgtatt tgctccactg tacgcataaa gaccaccgaa aacagctgac tgtgttcgtt
     4261 taatgatgcc cacgatactt gagaattcaa ccgtttgaga ttttgcaatc aatttggtac
     4321 gattttggac atccatattt gcagtgaccg cagcaatatg tcttgcacca gggcgaatga
     4381 aataattcac atacctaatc agtgcatctg gctccggtac cgtgtctgca tcattgctta
     4441 aaatcaattt cccttttgca aaggcaagtc cgatattaaa cgcgtgggct tttcctttat
     4501 ttttttcgat ccgaaccaca cgaagattag cgtatttttt catcagtcta gctaatattt
     4561 ctggtgtttg gtccgttgaa ccatcgtctg tcactaatac ctcatagttg tgataattca
     4621 atttcgtcat caaatactcg attgtatatt cgatcacaat ttcttcatta tgggctggca
     4681 ccatgatcgt gatgaacggt tcgacactca agggaacatc tacccattct ttttgcttat
     4741 gtttgtataa aaacacataa caccagacac caataaacca tacaaatccg cctaaaatcg
     4801 gataagaaac gagaaaaaaa tacataatag acaaacaagt atctaagaat aaattacctg
     4861 tatgggtgat tgagatcatt tcagttccac cccattttct ttatacaaat ctgcaatgaa
     4921 tgtcgtatct agatttttct cttcggagac agaataaaaa cggaccgttt ctctctcttc
     4981 ttttgttcca aaacgttccg tatacacttc ttcaagtaat tcttgtcttt ttttcaattt
     5041 ttcatcttca tattcaaaag atttatttac ttttttgtat agatttcgat tgttccacct
     5101 agtcaataca aatgaggtaa tcaggaaact gaaaaaaatc aatacaaaaa agccgattaa
     5161 gaaatacagc attttctttt ctgataaaaa agcgtacaag gaaaaatcct tggctctttc
     5221 tggtctcgtt aatgggaaaa agacccatat aaaaggaaga acaacgatca tccatcctaa
     5281 aagagccact aacgtttgcc aaatctttaa ccagtaatta ccttttgaaa aatacttatc
     5341 tttcaccaaa attgatgcaa cttcttctct tgttactttt ttcatatcct cacctcacgt
     5401 ttttttgtta cgacgtagga gtttaccata catttcattt cttttcacat aaagtgttcc
     5461 taaatttttc ctaagaaaaa gaacaaaaca tttctcatca gcaaatgaaa aatattttgt
     5521 tctttttatc agttgttctt tttatttttt atctcttaat tatgttgctc tttccaatac
     5581 gacaatgctt ttcattcctc tagatatcgc cgtgtacatt tgtctgcgtc ttctttgctc
     5641 atctccatcg ttttcaaatg gatataagat tacatgatca aattccagtc cttttgctag
     5701 actcaaagga atgatttggc agttttctaa tccatatgaa ttttttttaa tcgattcttg
     5761 tagcgtttgg gtttcctctt ctgtttgggt gataactgcc gctttttctc cttttagaga
     5821 aaccaaaatc tcagctagta ttttcaacaa ttcttcagag gatcgaaaag agcggtattc
     5881 gggttctttt ccttgcggtc ggatcgatac gatgtcgatc ttctcttgat ccacagcata
     5941 ggttttgaaa agctctgtga ttgctccgct agaacggtaa ctatttctaa gatcataccg
     6001 atggatcggc agatggtagt cgtcgaaaca acgcataata tcagcaaaag tagtggaaga
     6061 attaaagatt gcctgattct cgtcacctac taaagtaaag tgagtttttg gaaataattc
     6121 gatcaataga cctaattgtg ccagggtata atcttgtact tcatcaatca atagatagcg
     6181 aaatgctggt acatttacct tttcaacaag taaatgacga atagctaata taatgactgc
     6241 ttcatcaaca gtacgtgtgg gttgccaagc ataagctgag ccatatcgtt tttcataaat
     6301 tctttcaaac aactgatgct catccaccca tgccatttct tcgatttgtc tggttatttt
     6361 tcgatatttt ttttgtaaaa gtttccttgc ataagctaca atactttgtt ccgaatcatc
     6421 agaaatcaat ttaccgaata attccaactg cctatcttct gtcaacgaga gtacctgatt
     6481 ctgcacagca ggactttttg cttgtttcaa aagatgattc ttccattcgc tcatcagtgc
     6541 ctttttcgtt gcttgaagcc tatcgatcaa tttaggatga gcaggaactt tctcatatat
     6601 tttttcgatc gtttttttag aaaggactgt ttttccattt ttagtaagtc cacggataaa
     6661 attcggatga tctaaaaaca aaggatctga ttgttttaga ttatcgataa actttttgct
     6721 acgcaaacgt tctgtctgct ccgatacatt ttctcccgtg atacgtttga aataagcctc
     6781 ttcaccttct atttcctctg ctgaaagctg tgaaaccagc tgtatgatgg ttagattcag
     6841 tggatttctt tcacctaatg atggtaggac attcgaaata tagtcgataa accgatgatt
     6901 aggtgataga atcagcaagt catcggatgt catcgtatct cgatataaat agagcagata
     6961 cgcaatacgc tgcatgatag cagaggtttt accacttcct gctacgccat ttaccaagat
     7021 attttgactt ttcacatccc gaatgattgt gttttgttcc gattggatcg tagcagtgat
     7081 agccttcatt tcattggtct catcttggcc taagacttct aataatacgt catcttgaat
     7141 agctacagac gtatcaaaaa acttgaccaa acgatctctt tccaaaagaa attgtcgtct
     7201 ttctttaata accgtatcga ttttattttg acgaaccgta taagaagatg gaccaatcat
     7261 gttattgtaa aaaagttcgg caattggtga acgccaatca taaacaagat tttcatctgc
     7321 atcatctgtg aaatttgctg taccgatata aaaggattct tttggctcat tatccagaaa
     7381 gtctacaacg acttttccaa agtagggact tttcaataaa cgttcgatct ttgctagctg
     7441 attttctgcg tttttctgtt tgatattcat ctgatcgatt tctctatttt tcatttcaat
     7501 catcgaaaaa gtatccaggt tatctaagta actgtcaaaa ttcaatcgaa gctcgccagc
     7561 catttctttc aacgactggt ttccttctat ttttgccgta tccatcgatt cccgcagttc
     7621 gtttttttta tcaattagct tttgatacac gttcttcaga tatgcttctt ctaactgttt
     7681 ttgtgtgttg accatacaca ttctcctctt tttttaaagt ggtggaaccg attagtttac
     7741 ctcttttaag agtcgaatgc aagcttttta acgagtatca tttgttagga tatatcagtt
     7801 cttcaggtcg gacagtttga tataaagaaa atgcaccatc aaggtttttt acgtggtagc
     7861 cagactgctt caagattctt tccgctagat agctacgtaa tccgctatga cagctgacga
     7921 tatattcttg ttggctgtct aattcattta atcggtcccg aagttcattc aaagggatat
     7981 gtttggcttc cttgaatttg cccttttcca attcttctgc tgttcgcaca tctaataaaa
     8041 tttttccttt tgctagttct gtcggcaatt catgccattg tacggtttca ctaagccctt
     8101 ctgcaatgtt cattgctgca tatcctagca tgtttactgg atctttggct gagccaaatg
     8161 gcggtgcata cgtcaattct aattccggta aatcgaagat cgtcaaatgc cctttgattg
     8221 ctgttgctaa aatatcgatc cgtttatcca caccttttgc cccaacacct tgtgcaccat
     8281 aaatctcgcc agtctctgga tggaagatca acttcagtaa aatatctgtt gcttctggat
     8341 aatagcttgc atgatctttt cctgatacat gaatgacttc aaaagacagt tcgttttctt
     8401 tggctgttct ttcacttaaa ccggtggatg cggcagtcat tccaaacgtt cgtataattg
     8461 ctgttccaat accgccttga tttcttcgag aaattccgga aatcgtgtcc gcaacttgtc
     8521 taccttgacg attggctggt gaagcaagag aaataagtgc atcttttcca gtgatttgct
     8581 gttttacaac gatagcatcc cccaccgcat aaatatcagg aatattcgtt tgataacgtt
     8641 cgtctactag gatacccccg cgtatgccta attcgattcc agcggtcact gctagctggt
     8701 tatcaggacg aacaccaatg gataaaataa ctaaatcagc agggatggta cgcccatcat
     8761 ccagtcgcaa tcgtttcccg tggtcttcaa atccagcgac agcatgagaa gtaatcactt
     8821 ctactccttg atgagacaat tctttttcta taaaagctgc catttcttca tccagtgttg
     8881 gaagaatatg tggtgcttgt tcgatcacca tgacttctaa acctcttctt tttaggtttt
     8941 ctgccatttc caatccgatg aatcctgcac caacgatcac ggcacgcttt ggctgttttt
     9001 ctaatgcatg catcacttta tcgatatctg gtacatttct aatagaaaaa acattgtcgg
     9061 cttctgttat ccctggaatc gatggaacaa ctggttttgc accaggagat aaaaccaatg
     9121 catcgtactg ttcaatgtgt ttttgaccct catgtacgac ttctaccgtt ttgttttcgg
     9181 gaaagatggc tgtcacttca tgatttggac gcacatctaa acaaaaacga gcggataatg
     9241 attctggcgt ttggacaaga agattttccc gttcactgat ttctcccgaa agataataag
     9301 gcaagccgca atttgcaaaa gaaacatacg gtcctttttc aaaaacaacg atttctgcat
     9361 cttccattaa tcggcgaagc cgtgtagcag cagacattcc acctgctaca cctccgacaa
     9421 taacgatttt catcttattt ccctcctact gttcgatcaa accattgatt catgccacca
     9481 cgtacattca ctacatcata accagatttc tttaattctt tagctgcttg tttactcctc
     9541 atccctgatt gacaaatcac atagacggtc ttttctttgt ctccatcata tctgtttata
     9601 ctttgtaatg gaacattttt agctcctttt atatgcccgc cccggtattc tgcaggtgtt
     9661 ctaacatcaa gtaacgctgg attttccaaa agcttttcct ttaattctgg gactgttatc
     9721 gattttattg attgaaatag caaattcatt ttctgcacct catttacatt tttataaata
     9781 cctcatgggg tatattaaac aggagcttta ttaatatgtc aaccagaatg actcttcatt
     9841 tttttcacta ctttttatat ttgcataaaa taccgataag gggtattatc taattataaa
     9901 aatagaggag aatgtgttat ggagattgat aataaaaata tcctgaaccg tttgaaacgt
     9961 acagagggac aaattcgtgg tatccaaaaa atgatcgaag acgaaaaaga atgtatggat
    10021 gtcatcactc aattaagcgc tgttcgttct agtattgatc gcgtaatggg catgatcgtt
    10081 gcagataatc tgaaaaattg ctttgagaaa cctgatagta atccagaaga acaagcggct
    10141 aaactagaac aagcgatcaa gatgattatt aaaaagtaaa ggaatgattt caatgaaagt
    10201 cgttttccat attgatgaat tagaaaaatg gtcagaaaca gggaaaaacg taaaaaacct
    10261 gataaaagct tctccagaaa cgacgattgt cgtatctgta aatggcatcg cgattaccgg
    10321 ttatctagac tctgccaatg cagaattctt agatttgcaa ggagtcgttt ttcacgcttg
    10381 cgccaatgct atgcgtgcta atcatatttc agaaagttct ttacctgaac aagtaatcgt
    10441 tgtaccagca ggtgtccttg atttagttga actgcaatcc caaggttatg cttatatcaa
    10501 gccttagtca gtagaggttg cgacagaagt gatcagcttc aagaaatttc ggcttatttt
    10561 tcgaagaagc tacttctgga aaaccgtgaa tcaaaggttg tgaaaacgct gtcctgcatc
    10621 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttgggcttat
    10681 taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgaaat cagcggtttg
    10741 acctaagtat tagagaaaaa aacgggaaaa tagctcctca tattttgccg ttttttgagc
    10801 taataccgaa ggtcagctga ttgaacaccg tttaaggaag agattgtgaa aaagtcgctt
    10861 agtttcgagt attaagaaaa attttgtgaa aatggatttc tacatttttg cataaatttg
    10921 gcttaatacc gagaaactgg cttttgaata ccgtttaagg aagaggttgt aaaatcagcg
    10981 gtttgacctg agtattagag aaacaaacgg aaaaatagct gctcatattt tgccgttttt
    11041 tgagctaata ccgaaggtca gctgattgaa caccgtttac caagaggttg tgagcctacc
    11101 atttaactcc gaatcacaag aaataatttc aaaaacaact tctcctgttt ttggttcggt
    11161 tggcttatta tcgagaagta gctactggga gtctatttat caaaaaaagg aatgcgacat
    11221 aggtcacacc ccttttttat tttctttgaa acatctttcg gtactcgaca gcatacaaga
    11281 caaaagattg taaaagaccg taagtgatga cggcaaaaat gactctcacc cacagcgttg
    11341 gtaattctgg gaaaagcagc aaaaggatca tgccaacata gatgacagca gtcgctactt
    11401 tgccatacca cttcgctcca cttaatttga tatttttaga tagcccgatt aatccagcta
    11461 caaacataaa gccttctttt atcaagaata gaccaaacaa cagccaaagc cacggtcgat
    11521 accatgccat agacaaaatc agaaaaagtt gtgtcaattt atctgcaaac ggatcaagca
    11581 ccttccctaa gtctgttacc tgattatatc tccgagcaat gaccccgtct agataatcag
    11641 acaaacctga gacaacaatg accgctcctg ctatataaaa tgactgtctc agcacaaaat
    11701 aaagataaat cggcagcaaa aaaatacgga atatcgataa aagattcgga atcgtttgta
    11761 tttctttacg ccagtctgtg cttaatttca ttttatcgcc ctacctcctc agcactcttt
    11821 tctttttatt ttagcttaca cggggaataa aaccaatgat ttcactgttt ttatttttct
    11881 ttacagacac tgatattatg agctaatttg agataatcac ttgctgtttt catcgtttac
    11941 tcttctttct tcctgtcgtt tgcgcatttc ttttactagt actttatgag ctttttttga
    12001 tcctcgctct aggtcacgct gccgtttacg tgttttataa tcgataaaca gactgtctaa
    12061 atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc agacgaacac gctgacgagg
    12121 cacgtggatt atttttttgt ctaaatatac atcaatcgta tcttcaccta tatcttcata
    12181 aactaacgct tctttttggt attcattgac aaatatccga tcaccttttg caaagagctg
    12241 acgttctttt ttattctcct tcctatttgt aactgctgtc ttaggaagaa attctttttt
    12301 ctgtgtccga taggcttttt ccgccaagta acgctctgct tgatggatca atttcatcga
    12361 catatccatt ttatgagcaa tccacaaagc ttggctttct cctgtttctt ccaacccgca
    12421 actgatattt aggacgaagt gcttcccgat cgaaagccat agcagccggc acaaaatctt
    12481 catgatctcc ggcgaatttc ttgatttctc cataatgggt cgtggcaatg atcaatgccc
    12541 cttgctcata catagattcc atgattgcga tcgctagtgc agccccttca tttggctctg
    12601 ttccactgcc aatctcatct aaaagtacca acgtattcct ttttatcttt ttcaaaatag
    12661 cagcgatgtt ttgcatatga ccagaaaatg tactcaacgc attttctaaa ttttggtggt
    12721 caccgacatc gacgaaaatc tcatcaaaga cagcaagctc tgtcccttct tttgccggaa
    12781 cttgcatacc gaacatcgcc atcaaagtca ataaaccgac tgtcttcaac acgactgttt
    12841 tgcctccggc atttgctccg gtgataatca agcctcgata gtctttaccg atttcaacat
    12901 ccaatggtac cgcatgctca ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt
    12961 ccgatttatt gattctaggt gtgcttccat tgatttctcg gctaaatttt gcccgagcaa
    13021 agatgatatc taacaccgtg atcgtttcga tcaataaatc gatcacttct tcgttttctg
    13081 cgatagcacc agtcaatgcc gccaggactt gatattcttc actgatttct tcagctttta
    13141 acaactgata gtgttcattc aatttagaaa tcacagttgg ttcaataaaa accgtcgttc
    13201 ttttatttga ttcatcgata atcgttccat ctattttatt tttatagctg gctttgatag
    13261 gaatcgtata gtattcgcct ttttgaacaa tcattgcttc ttggatcatt tctttgtttt
    13321 tcggatgacg caagaatttc agtaattttg actggatttc tttttcgatt agttgtaatt
    13381 gtttgcgtac tttacgcaaa ttacgtgacg cgtcgtcact gactttatta ttttttattt
    13441 tctggtcaat caactcttct acatttatca aatcaggaag atgtttgcta taagcaaaca
    13501 acaagggtgt ctggtattga ttcttatcaa aaaactttgt gatcatccga ctgcttctaa
    13561 gaaaatccgc atattcgatc aaatcagttg gctgcagaat caaccctttt ttgacttgtt
    13621 ctgtcagtgc atcgatcctc ggcaacccca taaatggaac atgctgactg ctttctaaga
    13681 tcaagcgtgc ttctcttgtt tcttcttgac gatccaagac agtttgtagg tttgtcgaga
    13741 ccgtcatatc gctgattcgt tttttgctat agtttccaat tgctcttgct tggatttcct
    13801 ttttgatccg atcaaattgt aattgactaa tcatttcgtt gtccatattc tctccttttc
    13861 ttgaagagat acctgtccca aaataaataa gggcctagag ataaataaaa ggatagaaca
    13921 cagcttaaaa aagtcatgtc ctatccttat tagaatacaa agatattgtt ttcctgacag
    13981 gtaccgtttc ataagcacac caaataaact ttgtttattg gctattcgac ttactgatta
    14041 gataccagct acactcacaa aacatacccc attcttctag gtcttattta ttttccactt
    14101 aactatagca agacacattt tggttgtcaa actttagtat gttttataga tttacaccat
    14161 taatgaatag acacctaccg cgataattgc tccgatcatt ggacctgcga ttggtaccca
    14221 tgaatatgcc caatctgaag ttcctttatt agctattggc aataccgcat gtgctaatcg
    14281 aggaccaaag tcccgagcag ggttgattgc atatcctgtt ggccctccta atgaaagacc
    14341 gatggctaag atcaatccac ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg
    14401 tccaaaagct agcaacccaa gaactaatac aaatgtacca attaattctg tgatcacatt
    14461 tgctggataa ttccgtatag caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc
    14521 tttcgttgca ttccaatgtg gcaaataaga aagccaaaca agaatggccc ctgcaaaagc
    14581 tcctaaaact tgcgcaatga tgaaaggaac gacaagattc caactaattg cccctgtcat
    14641 cgccatagct agagagactg caggattcaa atgagctggg cccatgaatc cagaaatata
    14701 gacagccatt gttacagcga gtccccaacc aaatgcgatg acaatccagc cagaagcctg
    14761 tgctttactt ttgtttaaat tcacagccgc acacacacca tctccaagca aaactaggat
    14821 cagtgtccca ataaactccc ccatgatttg agtcatatca ctgttcattt atttctcccc
    14881 ctttagttcc cgcagatctg attctgcaat caatgcttct aaacgtttag tctgttgcgc
    14941 cttctcctct tctgtccatt caaaatatcc agcaattgca tcgatcacag gttgcttgat
    15001 ctcatcaagc tgatctcgtt caaacaacag atgatttgta cgacgaatga gataatcgcc
    15061 tggggccaaa accatttctt cttctaaacc ataacgtaac cgagctgact cagcgagact
    15121 caaaccagga taaggtgcca tttcttttgc taattcaaag atacgtcggg cattagtccc
    15181 gtaaaaatca gcgatataag tagcatcttc ttcctctaaa ccggctgcta ctccttcttt
    15241 tgctaattct gtcactgttt cttctaattt agttggatcg aagtttccac ctgaaatctg
    15301 atatttttta gagtcgattt ctttcgtctc tattccgtat tcttctttta ataattggcg
    15361 tatcaatcgc aaagctcctt ctgccatctt acggtaatcg gtgattttcc caccagataa
    15421 agtcagcaaa ccatctggtt ctctttctaa agaactacct ctggaaatcg tagaaggtgc
    15481 tttttcatca cggctgtttt ccaaatggtt caacacgtct tctacttcag cacgagaaac
    15541 tttgttttcc ttatattcac ttactgtatc gacaacttta ttgaagctct tgtctgaaat
    15601 cgatccatta tctccaccat tgtaatcaga accagaatta ccaatcaaca gtggacgaag
    15661 tcctgcccag cttgcttcga tatcattcaa tgtgatattt gcttctggat agcgatgatt
    15721 gatcacatcc aatagataat ctacatcttc ttgtgtgact ttcggatccg taaaatctcc
    15781 ttggtaatcc gtatctgtcg taccaaagta agtcttgttt tctcttggaa tagcaaaaac
    15841 catccgccca tcttgctttc ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt
    15901 cgcatctaca actaaatgga tccctttagt tggacgcatt ttaggagaga ctggacgcgt
    15961 aaaattcaag tttcgtactt tatctaccca aggaccactc gtattgatca ctaattttgc
    16021 tttgatttcg atcacttcat ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc
    16081 gccatcatat aaaaagccaa ccgctttcat tttacttact agataagccc catcttcggc
    16141 agcctttttg atattatcaa tcactaaacg ggcatcattg ttgcggaaat ctagatagac
    16201 acccgcgcct tttagccctt cttttttcaa aaacggttct ctttccaata cttcttctgg
    16261 tgtcagcgta taattctcat atttggttcc tgtcacattt gccagtttgt cataaaggtc
    16321 cattgctact ttgaccgaga acatattgaa ggttgttgct ccttcgccct catagattgg
    16381 caaaagcatt ggatctggtt ttgggatgtg tggggcaata ctttgtacga ctgcacgttc
    16441 accaactgtg tcagctacta cttccacatc aaatgtcttc agataacgaa taccgccatg
    16501 aacaagtttg gtcgagcgag aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc
    16561 tgttttgatt cctgatgctg ctgactggac tgccacaccg gcaccagtga ttccgccacc
    16621 gatgatcaac agatccaatt cttcttgctg cattttttga atgctatctt gtcttgtctt
    16681 gtttgaaaac atatggattt cctccttatt caaatgcttg ggtagctttt actgcttttt
    16741 tccaaccgtt gtaaagtttt tctcggcgct cttcctccat ttgaacttca aatagcttgc
    16801 cagcttcata aaattcgcgg atctcgtctg tatctttcca ataaccgaca gctaatcctg
    16861 ctaaaaatgc tgcccctagc gccgttgttt ccaagttttc tgctcgttgg atcggtacat
    16921 tcaagatatc tgtttggaat tgcatcagat attcattgtt tgctgcaccg ccatctactt
    16981 tcaatacagg aatcttgatt ccagtgtcat cttgcatcgt atcgatgata tctctcactt
    17041 gataagcaat cgactgtaaa gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca
    17101 agccaaacat agcccctcgt gctgcctgat cccaataagg agcacctagc ccaacgaatg
    17161 caggaacaac ataaacctca tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc
    17221 tagctttttg caacatttga agtccatctc tcagccattg aatcgaagaa ccagccacaa
    17281 aaatacttcc ttctaaagca tagtaaacct ttccatttat gccgtaacca attgtcgtca
    17341 ataaattatt tttggataat tgcggttttt caccggtatt catgacaata aatgaacctg
    17401 taccataagt attcttgacc atgcctggtt cgaatgccat ttgtccgaat aatgcggctt
    17461 gctggtctcc tgccatccct gaaattggtg cttcgcttcc ataaaaatga taaccttgcg
    17521 tataaccata aacttctgag ttagaagtga cttttggtaa catcactcga ggaatattta
    17581 gtaaatctaa aatctcctga tcccaatcta gctgatggat attgaacaac atcgtccgac
    17641 tggcattcga gtaatcagta acatgtgctt gtccatcggt cagtttccat actaaccaag
    17701 aatcgatcgt tccaaacatc aattcgcctt tttcagctcg ttcttgcgcg ccttctacat
    17761 gatcaagaat ccagcgtatc ttcgttgctg agaaataggc atcgatcact aaccctgttt
    17821 tttcgtggaa aaattcttga tagccttcat tttttaagtt atctgcaatt ccagctgact
    17881 gtctggattg ccaaacaact gcatgataga ttggacgtcc tgtatctttt tcccatatta
    17941 ctgttgtttc acgctggttc gttatgccaa tcccagcaat ctgatttggt ttgatccctg
    18001 attcgatgaa cgctccggca ataactgact gtacagaatt ccagatttca tttgcatcat
    18061 gttccaccca actttcttgc gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt
    18121 gtcttccttt cttgtcaaag ataattgctc gtgagctagt tgtaccttgg tcaatcgcca
    18181 tgatatacgt tgattctgtc attgtgattc cctccattat atgcaaacgc tttctttaac
    18241 tactcttatc atactactgt tttaaaaaaa gaaactgtca aaattttatt ctttttttct
    18301 ttttttgaca gttttttatt atgaagctat tttttaatca attcttttct tttttcagcg
    18361 tctctattct tcttttcaat cgaataatgt cgtaaggtga cgcaaattct gacagataat
    18421 attcacactg ttttttgttt ctctggcaaa atgtgatcac caaatcatat tctttacctt
    18481 cttcataact tccaatctca ataccgccga ttcccctcag ttctctgatc agatattgtt
    18541 gaaaagcaat acgataaatc ggcagacttt ctaaatcgat tccaatcact actggttttg
    18601 caatataaaa atcgatcatc aaaagaacat tggcatactg gatcatcagg taactaagct
    18661 cactatcttc agctcttttc ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca
    18721 gtaactgtgt taaggaatgc ccgagcattt tctgctgctg ttccaaaagc cgatcacgct
    18781 catagacttc gatctttcca acaaagaaaa accattcatt gttgatctgg gaaatttggt
    18841 agcctactgc cttttcttct ttgattttca aacgacgagg acgataatgc cataacatcg
    18901 tttcccgaat gtaagtatct aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat
    18961 actgataaaa tatttcttcg tctactatcg aaaaagaaac aaaaaagctg taaaacatca
    19021 ttgtttcgta tacattcagt tctgctgctg ttcgactaag atgcatcacg atgattggat
    19081 cgattgcttg ataaagccgg tccgactgat agagtaattt cttttctttc aaagttgcgt
    19141 acgtactttt ttcattcaat agtctttttt tcgtaatccc gagccagcaa gcaatctgtg
    19201 cttctgctga tgccgtaaac gttgtttcta atacacggtc caatcctaga atgaacggtc
    19261 ttttttctgg tgtattttgc aaaaaaagcg gtcgctgatc ttccgggata tattggaata
    19321 gttcataata aaaatatcgt atctgtagct cttctccgta taattgaccg tttttgattt
    19381 gtaatccaaa ttcctctaag agctggttca attctttgat ctttcgaaaa acagaagact
    19441 cactgatatt aaacgctgtc gctaatcgaa cgatggatac ttgctgatgt tctaataaat
    19501 agacgagcat cttaaacttc aagctttcct gcaataaaaa caatagaatt ttttccaaac
    19561 ttacatcttc gtccaacaca agcagcgcct gatactcatt tcgctgtact tctacttttt
    19621 tttccatcat tcggccaatc aattcaatat cttccaaata ttgatcaaac gaggcttttg
    19681 acagttggac atgctctctc aaaccattga tagaggctgt tcccccttgc agaacaaggt
    19741 ttcgcaaaat caggatctgt aactgctctt ttctttctaa taatgcttca atcttcatag
    19801 gcagtattcg ccacctttga ccacaccata tctggcacac ggtattctgg ctggaaaaat
    19861 agctcatgtt cttctaactg ttcaaacgtc catccttctt ctactgcaat tgtcagttgg
    19921 tcaagaaggt ggaacatctc ttttttgctt tggagctgtg ctccaagaat ctttttttca
    19981 gaatcaaata gaaatttgag tttaactttg ctttgattta ttgctgtaag cttgtagtct
    20041 acgtatcctg tttcaatttt ttggggataa aaaatgctct cttcttctgt caaacctacg
    20101 cttcctaaga aatagccaaa ccactgattg ccgactggac gataggttcc ctgatccttt
    20161 gtctgtgagc cactaattgt cttgctgaca tttcgtgctg tacggacagc gttatttact
    20221 agagaaacat acattttttc atttgttgga cgaaaagtta cttggatcgc atcgccaatc
    20281 gcataaacgt ctttttgaga cgtttgcaag tagtcatcta cccagatcgt tccatcatca
    20341 ttcaatgtta attgttcttc ccacatctga ttgtcgggac gagaatgatt agccagcaaa
    20401 acaagatcat agttctcctt tcttttttcc gtctctacaa tagcggtttc attttcggtc
    20461 aaagacacaa cttgttcttc taaaaaaagt gaaagccctt gatttttcat ttcctttact
    20521 aacggttctg tcatttcagg atctagataa cgaaaaagaa ttgactttcg gctttcgtaa
    20581 agatggattt gtttttttct tgatgccaac ccttcagcta tttcgattcc tgcttgtcct
    20641 gcaccaataa ttgcaatttt ctttgcattc atgatctttt gctgaagagc cgttacgttt
    20701 tgtatctttt taactgggtg aataagcgtt gattctgcag aaatatttcg aaacttttga
    20761 tttgaaccag tcgctagaat caaacgatca aaatccaatt tttttcctcc tgctaaaacg
    20821 actttttttt ctttcaatcc tattactgtc ttttctgtgt aaacatggat tcgatagcgg
    20881 tcagtcagtt cttctttcgt gatccaagaa agttgttttt cactggtaac ctttcctttt
    20941 aatatcaaat tgaacccgct gggaatccat cctaacttcg attgcttttc aatcaaatat
    21001 atttcacttg ttggcatcaa ttggcgaaga taaatagccg catgaatccc tgcaaacgac
    21061 ccgccgacaa ttacaatgtt catctgcatt cctctttctt ataaggtgtt ctactgctaa
    21121 cttctcaatc agaaaacaca gtattcataa ctcgctttca accttatgat acatagcgat
    21181 gcagcagaat caaccctctc catcataccg tgttctaggt ctgcttctca ataatgtact
    21241 agtggaccta attgaagagt atcatttctg aagagatgtt tcaaaaaatg tactcttata
    21301 aaagaaaaaa actcgccttt tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa
    21361 agacgagttt tagcgtgtgt aatgtaatag ttagaataga cacatcattt atttttaatt
    21421 ctttaatgct tcaacaactg cttcgcctaa ttgatgagca gattgaggat tttgccctgt
    21481 aagaagccgc ccatcaattt gaaccacttc tgaaaaagct gctccagact gatagtttgc
    21541 tcctttttct ttcaaagcat cttctgctaa gaatggtact tcattcgttg taccatttgc
    21601 ttcctcttct tcattactga acccagtcac tgtacgtcca gctaaaatag attttccaga
    21661 ctcatctgta actggcaaca atcctacaac accgtgacaa acagctgaga ccagcccttc
    21721 atttcgatag atttgtgaag cgactttccc aagatctatt gcttctggaa aatcccacat
    21781 tgttccgtgg ccaccagcat aataaaccgc tgcataatca tttgcatcca catcatcagg
    21841 acgtaaggta tttcccaagg cccgatttct gaaatcttca tcttcataat aggcccaatc
    21901 gatttcatcc atattcaaaa cactttgcgg ttctaaaggt acatatccgc ctttaggact
    21961 gacaaaatct gcatcgatcc cattttttac taatacatca taaaaatgag tcagttcact
    22021 taaccacaaa cctgtaggac gctcgatctt ttcgtacttt tctacatttg ttaatacgat
    22081 caatactttc attttcccat cctctttcta taaaaattgc gttattgata acgtgctttc
    22141 aagaaattat ataatgggct tggcaaataa agctctttat atgcttcttc aaaaggtttc
    22201 gttacaaatt catcctcacg gttttccaat aatttctctg cccagtcagg atcgacgatc
    22261 atttgctgtc caattgctgc aagttctgca tatttcaata cctgctctac atcttcgcga
    22321 ttgcggatcc caccgacacc aactaaaggg atctttcctt gaatcgtgtt atggaccact
    22381 tcaagaatcg atttatcgtt gtatttatca gaacgagcta ttcgatcata catatttaat
    22441 gaaacatgca gataatctag ttttgactca cgtaatcgtt caagaagcca gatcgtgtct
    22501 tcaaaacgga tacctggtgt ttcatattct tctggtgaga aacgataccc tacgatgaat
    22561 ggttttttag catattgttc aaccgctgag aaaaccgttg ttaataattc ttcaataaaa
    22621 cgataacgat tttccaagct accgccccat tgatcctctc gtctattcga atgcggagag
    22681 aaaaattgtt ggatcaaata cgtatttgct ccatgcaact cgattccatc aaatcctgat
    22741 tcaatcgctc tacgtgtggc tttgccaaat gcttggattg tttcaacgat ttcttcttca
    22801 ctcattgctc gtggtgtttc cgcatcagga cgttttgctg caactgcact tgcagataca
    22861 ggctgttctc cagataaagt tgctcgactt gtcattcgtc caccgtggaa aatctgaata
    22921 atcgcttttg cgccttctcc ttggattgct ttcgctagct cacttaatcg aggaatcatt
    22981 tcatcatgag cgatgctgag ttcacctggc cagccttttc cgatatcttg gacatttgca
    23041 gcaccagtaa taacagcacc aagaccttta gaacgttgtt tataataagt gatctcatct
    23101 tcggtgatca ctccattgaa aaagctgagt tgtgtcgtca taggcgacag catcaatcgg
    23161 ttcttcaatt gaactcctga aggtaaagta attgattgat caaattgtga cattgtcatt
    23221 cctcccataa ttcgctctga ttaacctatc gagaatcatc tatcattaaa ccactcgatt
    23281 agttgtacat gcaactaatt tgcttaaaaa attttatttt ttttgtaact tatctgttgc
    23341 atcattcatt gtctgggcta attctttact tgcttcattt cccaaaactg ctttgtatga
    23401 ctgatgaagt tcttcccagc taccatcgac ttccttagca aattggcgtc cttgttctgt
    23461 aagataaact aatgacaagc gaccttcgct aactgttttg acgtatcctt tatattctaa
    23521 tttttcaaca aaacgagcaa tcgttgatgg ggcggaatag agcatttgtg caatatcttt
    23581 ttgtggtatt ccatcttttt cttttaatat caataaaata aaaccataag ttggtgccag
    23641 acctgtcctt ctaaacgctt catcagctaa tttattcaac atgcgatcta gctttttcac
    23701 gctaaagaaa agacattcat ctaacgcttt ttcaattggc ttttccattt catcccctcc
    23761 attggcgtac atatcatagt atgattttta ttttttgtca aaaaacacgg aataatgtcc
    23821 gcctcctctg tctagatttg aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt
    23881 ttcgacaaaa ctccttctgg aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt
    23941 tgagtatgaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta
    24001 ataccgaaaa actggctttt gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg
    24061 catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttgtcg ttttttgagc
    24121 taatgccgat ggtcagctga ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga
    24181 actccgagtc acaaggagca atttcaaaaa acagcttctc atgttttatg actatggtga
    24241 cttgtgatcg aggagttggc aggagaatac tgtttattcg taaagaaggg gctgtgacaa
    24301 gacttttgtc acagtccctt atttcctttt attataggat ttcgccattt gattggatga
    24361 cttgtctgta ccaatcaaat gattttttct ttaaccgttt ctttgtgcca ttcccttcgt
    24421 catcttgatc aacatagata tatccataac gttttgacat ttgtgaagta gaagcgctga
    24481 cgatatcaat gcagccccac gacgtataac caaataaatc tactccatct ttgatagctt
    24541 ccttcatctg ctcaatatgc tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc
    24601 catcttcttc tactttgtct atagctccga gtccgttttc agctatgaac agtggttttt
    24661 gatacctatc atataattga ttcaaagcaa ttctcaaacc cttaggatca atctgccagc
    24721 cccattcact tgatggaaga tacggatttt taattcctcc agttattaag tttccttgga
    24781 ccccttccca attttctgga tgagcactga ctgcactaga catgtagtag ctaaaagaaa
    24841 caaaatcaac agggtaggct gctaagattt cttcatcacc tacttctatt tttaagttga
    24901 cacctttttc ttcaaacatt cgttgcgtat acgctggata atatcctctt gcttgaacat
    24961 cactaaagaa caaagaagtt ctttgctgct caattgtttt ttgaatatca tccggatcac
    25021 aagaatacgg aacaagaagc tgatagctga tcatacaacc gacttgtgaa cctttgataa
    25081 tttcatgaca agcttttgta gctaaagcac ttgctataaa ctgatggtgt gcaccttggt
    25141 atttcgcttg ttccaaatgg ggatggtttt cttcaattac tcctacgcta acaaaagggt
    25201 gatgttttac acagttgatc tcattgaaag tcatccaata ttttacttta tttttatagc
    25261 gtttgaatac tgtttcagcg aaacgaacgt aaaactgaat aagctttcga ttaggccatc
    25321 caccatattc tgtaaccaaa tataacggca tttcataatg ggaaagagaa acgactggct
    25381 caatcccata ttttagacat tcgtcaaaca cttgatcata aaaaaataat cccgcttcat
    25441 tgggtgtttc ttcatcacca tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc
    25501 atttaaaacc catttcagca aataaacgga tgtcttcttt aaagtgatga tagaaatcat
    25561 ttccgtgtct tttaggataa taatacttgc tgttgggatc ctgcgccgct tttacttgct
    25621 gatgcgtcat catatgccat tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg
    25681 tatacacatc agacacagat aatccttttt catctacatc ccatgcacct tcacattgat
    25741 tggccgctgt cgctccgccc cataaaaatc cttctggaaa ttttttcata tcgatccccc
    25801 taaacttgct agttttcttg tttgattatt tttcttatga ctagtaatga tatttttgaa
    25861 tctactacta tttgtaacaa taaccattgt ggtcggatca tatccctctt tgattaattc
    25921 ttgcaaatct acttccatca ataaatcgcc tttacgtact ttttttccat cagatactgc
    25981 actttgaaag tatttgccat ttaaattcac tgtatcaata ccgatatgga tcagtatctc
    26041 tgctccttct tcactgacta ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc
    26101 cccgtttaca gaactgtaga tttttccgtc cgttggaatg atcgcaaacc cttctcctaa
    26161 agatccacta gcaaatgttg gatcgtttac ttcagataaa tcaattattt cacctgctgc
    26221 aggagtactt aacactatat cctttgagga ttcttcttcg atttcatcct tgtagccgaa
    26281 aagaaaagtt agcatacagg aactgatcat cgctaagaaa taactagcaa ctagccaaac
    26341 aaatccttca ccaatataaa tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc
    26401 accggctcct gcggcaccag ctactccacc aaatatagct ccacatatac aagcaatgta
    26461 aaatggtcgt ttatatttaa gagtcacacc ataaacagca ggttcagtga ttccaaataa
    26521 tgctgtgata aatgcagata aagataaact ttttactttc ttatttttcg ttttcaaaaa
    26581 taccccaagt actgctcctg cttgcgcagc tacggcagtc atacatgctg gacctagcgt
    26641 atcacgaccg tagaaagaaa gattgttggt cattactggt acaagtcccc aatgtaaccc
    26701 aaaaatcact aacacttgcc aaaatccgcc gataacagca ccagataaaa cagggctaaa
    26761 gttataaaca gctaaatatc cagctgcaat cagctgactt gcataatctg caattggacc
    26821 tacaactaat aaaataattg ggaccatcac gatgatacaa atgaatggtg tcaataaatt
    26881 tcgaatactt tcgtgcaagc ttttgcgaat cttaggctca atataagaaa gaacccatac
    26941 agctaaaatt gctggaataa ccgtacttgt ataccttgct agaatcacgg gcatccctag
    27001 aaatcgcaag gttatgctat cactatatgc actgacaatc gtaggataaa cgagagctgc
    27061 tgcaataacc atagcgatga aacgatctgt attgaacttt tttgcagcag tatatgccaa
    27121 aaatataggc aagaaataga aaaatgcatc agctgctgca ttcaaaatga tataagttcc
    27181 tgattcagta gttaataaat tcaaactcgt acataaggcg agtactcctt tgatcaaacc
    27241 acttcctgcc aagacgccta aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga
    27301 gtttaaaatg ccttttttct cagttcctct ctttccacta gatgcatcat cttctagttt
    27361 catttgtttg gataatacgt catacacttc atttactgca tttcctacta cgacttgaaa
    27421 cattcctcca gcttcgacta cagtgatcac accttctagt tgctctacct tctccttatc
    27481 tgccttctta ttatctttta gggtaaagcg taaccttgtt gcacaatggt aaacattgct
    27541 gacattttct tcgcctccga caaatcggac aatatctttc gctaattgtg aatagtccat
    27601 ttttacttcc cccttgttaa ttaatgccgt agcaatcctt cttacaagat aagtatagtt
    27661 cttagaaaac ggtttctcca ttcaattagt accccttcta atagcgaacg aaactgtaca
    27721 atagtgtgta tggtttactt agccgagcaa atcactcact gcttgaaaga aaacaagtat
    27781 atcgtttctc ttatagtcaa aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg
    27841 tataagtggt atctcaagct gatactctgt gatttttgtt tctacttggc ttttgataag
    27901 acagcacaac aaagtctcgg catttaacgg aagcataact aggtcagaac taggagagat
    27961 tagttgctga atggaatcta tctttttatg gatttttatt tgattttctt ctttgtagta
    28021 atgatcaagc agttttgttt tctttttttc ttcaactatg gtcagctttt cacgcaaaat
    28081 ctgataaatc tcccaagatt caaactcatc tagaacacta ggtagataaa tcaaatcatc
    28141 ccactcaaaa ataagctctt gctcagtagt caaaattgca tcatattccg ttattttgtt
    28201 tgattgattt ttgaaaagat acactggttc tatccggcat tctttgatcc acatgttcat
    28261 actttgttga atctgttttt ttagtgtatt caaaatacta attggctggt tactaacaat
    28321 cagtatctcc ccttgttttc gaaaaggatt caacgcttcg gcaagataca aaacaaaata
    28381 agcgctttca gctaaattgg gacgtaattg aaaatgtatt gggaagaaac gccgaattaa
    28441 ataaatttct aaaggataac gggagatgat cttttgtaca aaatgattca tggtattatg
    28501 tccggcatcc atcctcgttt tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg
    28561 gaacaataac gaaacaggaa gatctaaaaa acgaataatc gtttgctcaa atgctcttag
    28621 attcttctct atttctttgt ttttttcatc attaatcatc aaataattta actctaatag
    28681 tcgattttca attgcttgtt tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa
    28741 cttttttgtg agggtctcta ccatcggaat aaataatgta tacctgtttg ttttttctaa
    28801 aagttcttct cttcgaagaa tggtcaaaac gagataccga gaaaatcgta tgaagtcctc
    28861 tcctgaaaca atataattat atgatataag aacctctttt aatgtctttt ggacagtcgg
    28921 catgatattg gaaaaaattg tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt
    28981 ttctgttcca acaagagata agaaaattct ttttgaattt tcttcagcaa tgatttttag
    29041 tccttttgtc gacgaaactt ccagttctaa tttaggattg cgctcgatcc aacgcaaaat
    29101 cgtttttatt tgtaatgata cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg
    29161 catactgaca tacccttttt gaaacaataa gtacgtcaca atagttgaag ctcggaaatc
    29221 catgctggaa tagattttat gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga
    29281 ttcaattgat tctaaaaaat aacctgaacg tttatttgac tggataatga ttttcttctc
    29341 gtagatagca agcatatttt tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc
    29401 ttcagctaat tcttcgcttg ttatccaatg attcttattt cttaatacta caagaatttg
    29461 gagttgtcgt ttatttaagt ccatttgctt acacctcctc tttgttataa acatacagga
    29521 aaaacctctc aaatgacaat agtcacttgc agaggtttaa aaatcatttg aagaaagatc
    29581 tatattcaag gtttttacca ttagactgga taatttgttt ataatattca aaactatctt
    29641 ttttcaatct cctaaagctt ccgctcccat cattttgtct atccacataa ataaagccat
    29701 atcgcttcct catttcccct gttcctgccg atacgatatc gattggtccc caccataagt
    29761 aaccaatgat ttggcaacca tcttttattg cctcagcaat ttcctgaaga tgtattttta
    29821 gatactcaca acgttttgga tcgtggattc gattcaattc atctggtgct tcatctagtc
    29881 caatgccatt ttcaactatg aataatggta attcataacg gtctgtcaat tcattcatga
    29941 cataacgaaa ccctagagga tcaattggcc aactccaagg ttcgggagat ttttccttca
    30001 aaaagggatt atcgaaacca tttgctccgc cagtatctac gcgcatttct gcttcttttt
    30061 tatagacagc gctacgataa taactaaatg caatataatc tgccgtgtta gcttgcaagc
    30121 actgttcgtc tccatctaac attactggtg cacagtcttc ttccttccat gtcctgtaaa
    30181 tataagctgg ataatgccct cggcacatga catcggagaa aaaccatgaa ttgcgtttaa
    30241 attgcagtga accaaataca ttctctggat cacagtcata tgcataagtc gctaatgcag
    30301 aaaagctaca cataataccc atcttagcat ttggatcgat tttctttaat aattgagtag
    30361 cacgggcgtt cgctagaaac atatgatgac aggcttgata aatcactttt tttgttagtg
    30421 tagcaatggg ttcaagttta tctctagcag ggaatgtaac caatccagca gctgcataag
    30481 gcatacgaaa agcattattg atctcattaa aggtcatcca atatttcact ttcccttggt
    30541 agcgttcaaa gacggtctgc gcataaaatt caaaaaagtc gattaatttt cgattttgcc
    30601 accccccata ctctcctacc aatgctaagg gggtttcata atgactaaga gtaattactg
    30661 gctcgatatc atatcgattt aatgtattga ttagtctgtc ataaaatacg agaccagcct
    30721 catttggctt ctgttcgtct cctcttggaa atattcgtgc ccatgagatg cttgttcgaa
    30781 aagcttttaa acctaactcc ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat
    30841 caatcgcttc atgagaaaga taatggagtg attcgtctaa agcaatctcc caaatctttt
    30901 tttcttcatt ccactgaata ctaggatgct ggttcatgat cccgataagc gtatctgtca
    30961 cattaggtaa tttgccgtct tccaaccatg caccttcaca ttgattagca gctactgctc
    31021 cgccccataa aaaattttct ggaaatattt cctgatccat aactatctct cctcgctatc
    31081 aacaatttct tacttgaagt atagctcgat tcatcaaaac aagatattct aactatttcg
    31141 gcagaagaga agaaaaaaat tgaatgctac tcttcatttt taatatttat atataacaaa
    31201 tacataagac gatccccgaa agaaaacact cgttaaccat ctaagcaaac gatttatttt
    31261 acttccaaca caaatgcttc ataaggtcgt aaatcaactt tgccgtctgg taactgtttc
    31321 agaggataat ttgacacaat aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt
    31381 tcactggaca tattacaaat aacgagccat tgctggtctt catatatgcg agaataggca
    31441 aagacatgct caggcgtctt ttccactaat tcatagtctc cccagacgat gatgtcatgt
    31501 tttctacgca aatcgatcaa tttcttgtac atataaaaaa cagaatcttg atctgctaac
    31561 gcttcttctg cattgatttc tttataattt ggattgactc gaagccatgg aattccagta
    31621 gtaaacccag catgttcacg ggcgttccat tgcataggcg tcctagcatt gtctcttcct
    31681 ttcgccttga ttgattccaa gatttcctct tttgtaaatc cagaagaaat cctttcgtta
    31741 tacatattga ttgtctcaat atcttttgct tccgcgatat catcgattgg gcaattcgtc
    31801 atgcctaact cttctccttg atagatataa ggggtgcctt tcatcatatg gagcaagata
    31861 gcaaacatct tggcactttc ttttcgatac acatcgtcat ttccccaacg agaaacaatt
    31921 cgcggaagat cgtgattatt ccaaaataaa ctattccatc cttcatcacc taaagatgtt
    31981 tgccatttgg ataatacttt tttcagttca gagatactca agggtttcaa atcccatttg
    32041 tctttgcctt cttgctgatc taatcctatg tgttcgaatt ggaagaccat ggataattca
    32101 tttcttttag gattggaata aagtttagcg atttcggtag ttgctcccca tgtttcgcca
    32161 acagtcatca gatttttgtc accaaaagtt gcttgattca tttcctgaag atactcatgc
    32221 agtttaggtc cattacttgt gatttcttta tccggttctt tgccaatcat atcgatcacg
    32281 tccatacgaa agcccccaat acctttatcg atccagaaat tcatcatttg ataaatctct
    32341 tggcgaacct gtttattttt ccaattcaaa tcaggttgac gcttgctata caagtgcaga
    32401 aaatattgcc ccgttttttc atccaactgc caagcagaac cactgaatat tgagcgcaat
    32461 ctattaggct cttttccatt gaccggatca cgccaaatat aatagtcccg ataaggattt
    32521 tctttatttt tacgggcctc aatgaaccac ggatgttcat cagaagtatg attcaccacc
    32581 agatccatga tgatacggat atttcttttc tttgcttctt caatcagttc ttccatttct
    32641 tccatcgtac cgaattctgc catgattgct tgataattac tgatatcata cccattgtca
    32701 tcattcggag actcgtatac tggagaaagc caaattgcat caatacctaa atacgccaga
    32761 taatccaacc tggaaataat cccccgcaaa tctccaatac catcttgatt tgaatcttga
    32821 aaacttcttg gatagatttg ataaacaact gaatggtgcc accatttttg ttccatgatt
    32881 ttcctcctat aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac
    32941 taaaaattgt aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca
    33001 aaaaaacagc tgaggaaatt ccctcaactg ttttttgaaa ggattgattt atgcagggaa
    33061 gaatgctgat ttcacaactt ctttataaac ggtgttcact cagctgtccc ttggtattag
    33121 ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt ttttctctaa tactcaggcc
    33181 agaacgctga tttcacaacc tctttatttc acattcaata tccacatgaa tcccacaaat
    33241 acaaagaaca gaccatacat gattggagat acttcttttc ctcttttagc tgctaacatg
    33301 gtaattggat agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc
    33361 atgccgatca agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt
    33421 cgcaaagatt gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa
    33481 gtaacaactg atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta
    33541 atagctgtga tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca
    33601 ccaacaggag aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa
    33661 gctttcccta cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca
    33721 actaaagttc ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac
    33781 atttgaagag aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa
    33841 cttggtgctg cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa
    33901 atcgttgtag ctgccatccc gatgaagatg ccacctggta cacgccgaac aagcaagata
    33961 gcagttacga ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat
    34021 cctactaatg tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagcg
    34081 ataaatagac cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg
    34141 atcatttccc gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg
    34201 ccggctaatg ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa
    34261 gcattgatcc caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa
    34321 atacagccga tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca
    34381 gaagcaccta atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta
    34441 aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa
    34501 agtatttttt ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta
    34561 aaatttcgat ttattaataa aacaacgtgt tttatcatat actaaaacaa aatataagta
    34621 aacaaaaaat tcggatttta agcaaaatcc gaacgtttca tctgattaaa tccctttatc
    34681 tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt
    34741 tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgtta
    34801 ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt
    34861 gtcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg
    34921 aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag
    34981 atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat
    35041 aattgccttc agggtccgaa aaattgaaag tcaagacgcc attgttgtct gtgatttctc
    35101 ctggttcttc catgttctcg tagatagtct caaattcgtc cgtaaaaaac actaaagaag
    35161 gcggtggtcc taatacttct ggtgaaaatt tctgcacaaa tacttttggg aatacgccta
    35221 attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg
    35281 tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat
    35341 tgacataaag catcacacgt gttttttgta tcatcgtttg acccttcttt ctatttgatt
    35401 ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg
    35461 atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt
    35521 ttacaaaaaa aaaaaaacgg caatgaatgc cgctcttctt ttttcgattc ttttataatt
    35581 ctgcttctga ttcatctttg gttagtgtgt gtctaacact agttacgcca aatgtttgat
    35641 cgattttttc cttcaacgct tcgattgcct gtgcgccacg tgccgcttgt tcagcattga
    35701 tggattcaat gaccagcacc gctgcttgtg tttcttccgt cagcatcgtt ctttgggctt
    35761 cacgccagtt gagacagcga cagatcgctc cttgttcatc atagtagatg atttcttccg
    35821 gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa tgattcgcca ccttgggctt
    35881 ttcctaaatg cagatttcct gcgatctttt cgatatcttc tccgccgcaa ggtacgccaa
    35941 aagacaagga aatactatta tagatatcta caagcggatt gattgggaaa aattcccgtt
    36001 cttgatttgc acgtttcagt aaagcttcaa tagaagaacg tgcacctttt ttagtcttga
    36061 atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt ttggctgaat gtttcttctg
    36121 taaggtaatt tcttgcttct tttgctgcct cattcaataa ttgatgatac gtttcttcac
    36181 tttcttttgt tacgtggtta tcgataccat ttacggataa gatttcgatg gttgcttcag
    36241 gaaatacttc ccagaaattg gtatccacta ttactttttt catatgttca cacctcttga
    36301 ttagaataat tgcttgatga atagatagtc tgtttgggga tcatctccca tataaaaaac
    36361 atgttcgctg tatttgataa atcctttttt acgataaaaa gcgagcgcaa catcgttgtg
    36421 ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct tttgcttttt caatagctgt
    36481 atctagtaaa aatgtaccga taccttgatg tttaaatttt tttcgtacat agattcgctc
    36541 gatttctaaa aaatgatcag ccatttcctc agattgactc gttcctgtat ttagtttcag
    36601 gtaacccgca ggctcttgat cgatactcac taggaaaaaa tcggtttctt tatcttttag
    36661 ctcttctgta agtttttctg gcgcataagc ttgtgtcaag taatcagcta aatgcttcgg
    36721 tgaattatct tttccaaatg tatctgtaaa tgtttcgata ctgatttgtt gtagtaagtc
    36781 tacatctgaa ggcgtcattt ttcgaatttc gtatttattc acggcttctt cctttcaata
    36841 gatcctttta ttgccttttt tgacaaaatt ccaatcttcg ctaacattct tgcatacttt
    36901 atttaaaaga tattccaatt gttttgcttc ttcgtcagaa agaccattta atgcgactgc
    36961 attggagtag ttgttttctc gaatgatgaa aggatagatt tctgctcctt ttttagtcgg
    37021 atagagtttc ttgatttttt tattttcttt atcttccaaa cgttcgatca tcccattgga
    37081 ttctaatttc ttgattgctc gggcagtcgt tgtacgatct actttgatca tttctgctag
    37141 tttttcttgg atgattccag gattttcgca aatacgtact aaataaagat actgtcctct
    37201 tgttaaatcg acttctttga attcgatatt gctgatagaa tctagcgctc gggcaatcat
    37261 tccgatgtct cgaaggattt cagccatttc atcccacctt tattttgttt gtaagactag
    37321 tttacctctt tttgttgcaa atgcaactat tttcaataaa aaaatcaagt tcttttttaa
    37381 taactagtaa agtatattct tttcttcttt tacgaaaaag ctcaatcaca atagatctgg
    37441 tttttgtttt ctaaaataag taactgtttc ttcgttgttc gcttttctga tagctttggc
    37501 aactcttcta tactaaaaaa tcccatgttg gaagtttcat tattctcaat aaacttggcc
    37561 tcttcaccgt ggatcgcaca agcaaaaaca agtttataat actgaaaaag ctgtggaata
    37621 tctttttgtt tattcgtatc aaaaactgct cgcaactgag tagtttctac tgttaatcct
    37681 gtttcttcat agacttcttt acgcacattt tcttcaggag acaatcctat ttcagcaaat
    37741 ccaccaggaa gtgaccattc tttcgtccgt ttatcttcga ttagtaggac cttgccatct
    37801 tttttgatat aggcacgaac atctactttt ggtgttggat gtccttctcc aatcgttgtg
    37861 agcgctttta tttcttcgaa agtttctttt cctatttcac tgatcagttc caatgaaatc
    37921 gtccggatct cctcaaaacg ctctttatca aatacatctt ttccataata tagaccagct
    37981 tgagaaagtg cgagtagtct tttgtattga gccaacttct tgttcattct cttcccttct
    38041 tttttagcaa atgtatgact atgtaatcaa gtatagaaga ctagtttaat tacggcaaat
    38101 aaatataatg aaatctgcaa taaataaaag aatgttaaaa aactcgagaa caaaagacat
    38161 tcgacttctc tggggctgtg aataaatggc gttcaaaagc ttgcatctgc ggtaataagc
    38221 cccaccaaac gaaaaatatg acgagctatt cttgtctggc tgttcttatt aattgaggca
    38281 ggacagcttt ttcacaacct ccccactaaa cggtgttcaa tcagctgatg ctcggtattc
    38341 actaaaaaaa cggcaaaata tgaggagcta ttttgccgtt tttttctata atactcatat
    38401 taaaacactg acttcacaac ctcttcctta aacggtgttc tcctaccaac tcctcagtca
    38461 caagtcacaa tattcatcaa acatgagaag ctgtttcttg aaattgctcc ttgtgactcg
    38521 gtgctgaacg gtaggctcac gacctctcgg taaacggcgt tcaaaagcca atttttcgat
    38581 attaagccat ttctttgtta cttattgtca ttgatttttt ttctaattta tccaatagtg
    38641 gagtgtaatc cacttgagta tagtctgtta catcaaattc atatagtgtt cctaactgga
    38701 atttgtcata ttttcggtcg gcgatgatct ttttgaatgc ttctttcgta atatgattgg
    38761 tccctttcgt tcgatcttcc aatacgtcac catagtgata atgatccatg atatagctca
    38821 aatgacgatc tgattcacga tcacgtttgt accgacgttg ccataacact tcaaagtctg
    38881 ctgttaggcg aatcgtgatc acctgatatc cgtatctatc tgctaattct tggagtcgtg
    38941 ctttttgctt atcactaaaa ggatactcgg acacaatcac tttctttcct gcttccatat
    39001 acaaatcaag tgctttgtaa tagaaaggat aaactttcgt cctttcttgt tccgcccgtt
    39061 cttccaaatt gttaaacccg atggattctg catagtattc cttgatttca tcgagcgtga
    39121 tggcatacat atctggaaat cgttcccgta gtttattcat aagatatgtt tttcctgtac
    39181 caggactacc agccagaaga atgaaatact ttttcatctc atttatttcc taagttaatt
    39241 ggactttcca agttcataaa cagtccaatc ttatgcttga caatttcttt tactgcttca
    39301 tttgctggaa tcacattatg gcgaagagac ttgttgattt ctgtttcact aaaggtttct
    39361 ttagctactt tcgtccagtt gactaaaagc tccgttccaa cattgaattt gcggatcccg
    39421 ttattgatca attcaggata atcttcttct ttcacacctg ttcctccatg gataactagg
    39481 gggacatcaa ctactgcatc gatttctttc agcaagggaa tgtttacttc tgtttttgat
    39541 ttgaattgtc catgattcgt accgatagca atagctaatg catccacgcc tgtagcttct
    39601 acaaatttca ctgcgtcttc cggtcttgta tataccttgt cattttcagc gacatggatt
    39661 ccttcttctg taccgccgat tgttccaagt tctccttcta cggaaacacc gtgcgcatgg
    39721 gcatattcta ctactgcttt tgtcttcagg atattttctt tgaaaggcag atgggaccca
    39781 tcatacatga cagaagtata tccgctgtcg attgcttctt tgatatcatc aaagtctcgg
    39841 gcgtggtcta agtgcaagac tgcatcaacc atttcttctt ctgcaatagc tgtcattaca
    39901 ttgactaaca catgcatgct gatatattta gcagtatcga cacttgtttg aataatgatt
    39961 ggcgaaccca tttcttttgc tgcgcctagc atggcaggaa gcatttctag gttatgcgta
    40021 ttaaaagctc caactgtaaa attcaactct tctgctgttt ttgttacttc tttcaatgtt
    40081 gtatacatct tttattgcca ccttatcttt ttatttgttt cctttgatcg ttacttgttt
    40141 tgcaatttgt cgcatctcat ccattttgcc catgtagtaa tcgatccctt gatcatcacc
    40201 agcacgagca gcatctgaac gcttctggtt atagaggctc atcagttttt tatacccatc
    40261 tccgatcgtc tgtttatttt ccaagctctt ttctaaatat tggatggctt gatcgttctc
    40321 atacaattca gaaaatttaa gtccaacttg ttcataaagt tccgctaatt tatgcctatc
    40381 ttgggtagtg gaaatctctt gtttcaattg tgtaatctct gattgaatct tttcggtcgc
    40441 ttctggcgat agggcttttt cttcttttgc cagaactttt ttcggttctt tttctttttt
    40501 cttaaaaaat ccaaacatca ttttccctac tttcaattaa aatgctttca aaactgggct
    40561 taagatccag gcatataaga gtgctgcggc tactgtatct acatcggttg ctgtagcatt
    40621 gatgaatccc atcgaattaa agatcgttac tagcaaagcg ggcaataatg taataaagaa
    40681 accatgagca ataccgccaa tgatcgctcc tcggcgtccg ccaactgcgt tgccaaatat
    40741 ccctgctgtc cctccggcaa agaaattggt tagcatacca gataaaatca tcgctaagcc
    40801 gaacatgggc aaagtaaaca tggcaataat cgtaccaatc gttgtcgtaa tgaaacctaa
    40861 aatgacagca tttgggctat aaggaaagaa aactggacaa tctaaggcag ggatcgcatc
    40921 aggaactaat ttcatagcta tccctcgaaa tgccggcacg atttcaccta acagtagacg
    40981 tacaccagct agcaatacat acacacccac tacgaattgg atggcttgta aaaaggcaaa
    41041 catgatatag ttttgatctc ctgataattc agaagcgaat ggttcgcctg caaataaaac
    41101 agtgatgata tatagcggga ccatcacgac catgacagac aaataagtat cttgcagaaa
    41161 ttcgaaatgt gtcggcaact tgatatcttc gatcgacttt ttattttcgc ctttctcacc
    41221 aaaaagctta gcgacaccgg cttcaaataa gtacccgatc gtacaaaaat gacctaaagc
    41281 aatatcattt gatccagtga ttttacgaat gatcggttgc gctactgccg gcatagcaat
    41341 ggcgaaacaa gctccgataa aaccaccgac caagatcaat acgatccctc gcagaccggc
    41401 aaaatagcca aagactgtcg tcatcgttgc catccataaa attgcttgac cattgataga
    41461 ttcaatagaa gggatgatcc cttgcatatg aaatccttca gtaaagattt tcccgaaata
    41521 agttaaactt ccaacgatga tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt
    41581 ctttaacgtg ccagagatga cttgtcctgc tgatttcttc tgcagtagta gacccaacat
    41641 agcaatcaat gcaatggtga tcgatgcctg agtcaaaata ttttcgatga taaaattgat
    41701 aacgctcatg tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga
    41761 actggggtga ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc
    41821 gccgttttag gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg
    41881 atcacatctg gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct
    41941 actcctaatt tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa
    42001 cctgcgccgc atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt
    42061 tattatttct caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca
    42121 atcaatcgat ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc
    42181 agatgcgagt aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca
    42241 ggattaccga aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc
    42301 ccatcttctg gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg
    42361 taatcattca ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta
    42421 accaatggtc tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc
    42481 atgatattgc tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt
    42541 tctaaattgt ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg
    42601 ccaccacttt ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg
    42661 ttaggctcta accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata
    42721 ttcgtctcag ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga
    42781 aaggtgctga aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt
    42841 aaaactgcag taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt
    42901 ccagtagcca tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc
    42961 ttgattacac tggcgatcgt caaaaagtgg atgacaagaa aagctatctc gtcctccagt
    43021 atcttgccac caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt
    43081 gggaaataat ttgttcggat gttttctttc aatggattca tgacttggat atgattctta
    43141 caatcagtgc agccatgtgc ttatagagat tttcgaataa agcttcttcc tttagatgga
    43201 aaggtacgtg tgtctcgttc tctacaaatt ggatcaattg gatcgttagt aattgagctt
    43261 gtacccactc gattgaattg gagatatctc tagcattgaa cgtctcaata gtgaaacgaa
    43321 gataatttct ttctacttgc gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa
    43381 taaatgcaga aaagctactg tcttttattt ctgtattttt ccaattaatg gcagagataa
    43441 attcatgttt cctgattcgc tggatccaga tcaatgtaaa aagcaggatt tgttttttat
    43501 aaatatcatc ttcttgtttc aacaacttat tggaatagat ctcctgtatt tttttgattt
    43561 ccaccaaagg aaaataatgg agaaagattt tttgagccgg tgttaattgt gcatcttgat
    43621 tcataaagaa gtcggtcatt cccagattct gattgataaa atcatagatc atcgtacgga
    43681 tagatcgttc tgaaccttta taaaccagtc cttgctttcc ataactgacg atctctatcc
    43741 cataactgac cagtgttgct cgtaaccgtc tcatatcttc atctaatgta cttttagaga
    43801 tttgaaattc ttcttctttt ttgtttagat ggatcgggat tcggctgtaa gcaacggcta
    43861 aaactagatc aaaaatacgt tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt
    43921 cagtttctaa tcgagcttgc ttttcctttt ccagcttcaa caaaaaacct ttgttgcgaa
    43981 ttgtacggat ctccggaagc tttttactgc tcaaccagat attcgcttca agaacttcct
    44041 gacggatcgt ccgctgactg acattgaact cacttgctag atgttttgct tttactggat
    44101 attgggctgt catgattctg tgaagtattt caattgttct tgttttcata gaaatgatct
    44161 ccttctgatt tcaagtatat ggttccgctt acataataaa aagtagatat ttctgccact
    44221 tgaagtggaa agaaagtaag caaagcaaac aaaaaataag ccatatgctt tttcatctcc
    44281 tcaacgaaaa agacatacgc ctattattac aaaaattttg cccatccata caaaaaagag
    44341 gttgtgaaaa agctgtcctg catcaagtaa taagaacaac taaactaaaa tagtgcttcc
    44401 tattttttgt ttggttgttc ttattatcgc agatgcaagc ttttgaacac catttagtgg
    44461 agaagttgtg acagagatgt tcagccttga gcgataagaa gcgattttga aaaatagctt
    44521 cccatatttt acaaaatcgt gccttattgc cgataggcta tctctggcac accgtttatt
    44581 cagaaaaagg agttgcgaca aggcttttgt cacaactccg ttcataaata tttcctgtca
    44641 atccagcata atcggctgaa taaaatcggc ttgccatctt ttttctttct cagaaaaatc
    44701 aaaataccat atctgagaaa aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc
    44761 atcttttcga tcgactgaat aatcacagca tctgaaatca attcggatgg caaaactatt
    44821 ttcgtggatc tgtcgataat ttcctacttc ctgaatagtt atttttttag gtatgatttc
    44881 ttacaccgtc tgctttgttt ttttgcagaa ctttctgatt ttcggcaaac agcttgtctg
    44941 tataacagct tctggctttt tcaggagttt cttcttccca agcggattgg atcttcatga
    45001 atatcttttg gatttccaga aactgtgctt tcttttcttt agccgttcct gacatgcggg
    45061 acaatatctc cgctttcagc cttttctttt gtcttttatt cttgacgata gaaaaagcag
    45121 aaacccctcc tactgacaaa acagctaatg caagaaatcc cgtttgctca taactagaag
    45181 gacctgtact tggaccataa tacccataag aatctctgcc gtaagaacga ctgccaaaat
    45241 aactgccccc atatgaacga ccgccaaatg aacctctgcc cgaacttcga ccgctagatg
    45301 tacctccacc atgaccaccc gcgacagcga atccttgggt cggcagagca aacaaacata
    45361 ggcccactaa caaaatgatt gctaaataat tgattttttt cattgtcttc ctcgttatct
    45421 tttattacaa aaaactattt ttatcatagc cttgctaaaa ggaagagtca ataaccagat
    45481 gcataaactg tttttttagc aattttgtct tatttagtca ttgagtaaca tcgaacagat
    45541 gtatgtgtcg gcagtccata attcctctat cttcagcatg aacaagcttg cagacaagag
    45601 atctgcgctt cctccaggag agatttggtt ttcctgacaa aagtgcaaca gtgctgtaaa
    45661 tgaacgattt ggtaaagcac tttgactagc agcatgagca agtttttgca tttccactaa
    45721 cgttttctaa tcactgcgat gcaatacgca tgtgtcatct agcgaactca tcaaaactaa
    45781 caacatatgc atttgtttct gtacatatga atcattatct agctccttag ctgcttggat
    45841 tgcaagcctg atatgcggat accctaatat tgcttcttca tatgcaccat ttacgccata
    45901 tttttgcttc accctatgac catgagtgtt ttttttatga cgatattttg tatcaggaaa
    45961 agcagcaagc ttgctgacat catcaagtat tttcgataaa gaaaaaggct gttccatact
    46021 gattttttgt gcaatgacac tgatcaatag ccccattgcc caaatcgctc ctttatgggt
    46081 attgatgcca tttgtagcaa tcagcattgc cttttccgct tttcgcccaa tctcagcaat
    46141 tctttctctt aactcctgat caatagcatg atgccatgct atttgagcca tttcttcaaa
    46201 aaaaggagtt agcgtcttag cagacttaag aaataaagca tagtccatat cggaatgtgc
    46261 acccgcatcc agtgcatcca ctaatccagg ttttggactt aatgttactt ctgcgatcaa
    46321 tgcttcctct accaacgcag cgagttgctg agtcactgct ttttttcgtt cgtcatttat
    46381 catgacactc actccttctt cctactatta cttgctgaaa ccttttttga tagctaccca
    46441 aatgatatga agtaaccagc ttaaagcaaa tgcacaactt gccgtcatta gataagctac
    46501 aagaatcatg actcctgaag gtaactgcag ccaactatct ttaaacaata ataacagtac
    46561 ttggaaaaca aatacattgg ctaaatacgt acgataagca taaacagaca accatttgaa
    46621 caatggcaat tttggcgaat cgtttacaat cattttggaa gcgcctatga agacagcaaa
    46681 gatgatgact aaactgtata aactttggat cgtattcaag taaggcgcat tcgcaaagct
    46741 caagtctcct ggatgtgcga gcaatgaacg gacagaaaag aacgcgataa ctaaggcaac
    46801 aggtaagaaa agataacgta ctctgtccaa atattgataa actttttcat gataaacaaa
    46861 tgcggctacc ccataaatcc cgtaaatcat gaaactagca acaaaccgat ctaacaagta
    46921 ccagctgtca tgataaggcc cagtcaatac ttgtgtttga tagaacagat accatgcgac
    46981 atataaaatc gttgaaccaa tcaatactgg ccatacatat tttttattag caaatacttt
    47041 gtaaccgagc caaacaaaaa acggcatgaa caattggatc tgcagcatca taacggtata
    47101 ccataaatga ggcgctccat cacctgtgac atatttcagt aaaaagcttg cagtatcgta
    47161 tacggagttt gttgttggac tgctggaaat agccaaagat aagccgtcgt ccatagaaga
    47221 tacggaacga ctagttcaaa aaacttttct ttcaaaaatt cccaataaga caaatgctgg
    47281 cttgttttta ccatattata aacaattgca aaaataaaca ttggtgctgt gtatttaacg
    47341 atgacgtaaa aggtaccaat aaaagctgta tttgttgcgc tgtggctgtt tgccaaagca
    47401 aagctcaata tcgtttgcat cataacagcc tttacagctg atactttctg gaagtcactt
    47461 acgttgattt tgattttttt gttttcaaac atctctttct ctctcttttt ctttattacc
    47521 aaagttcttt agttaattta ggtccttcga ctgttttcaa tagaattttt ttctctggct
    47581 ctctcaaata ctcttttagt gaaaaaccgc cttgccgagt cactacttgg gtatcgaatt
    47641 gctcaaaaaa agcaggatgc gattgaatta cctgaattgg caattcaatc ggtgaatttg
    47701 cgtacagtag caaatcaaaa tcactggttt cttttactgc cggaatccca gtcgataatt
    47761 caaatcctaa gctgccgccg attccccatt tacatcctga taatatgttt ctagcagcat
    47821 gaaattgctg ataaacagga taatccatga ttttctcttg tctatacgat tcttgttcga
    47881 tgatgtccca cggatgaatg acttgtttga cggcatgttt tgagatttct gttgcccatc
    47941 tctgattgcg ggcaggtcca cgtaaaccaa tcggtagtat ttctgcgtga tccgtcgacg
    48001 cacgacgaac tgttccgaac atcttttcag ttagaacgac ccattcaggc aaggtgaacc
    48061 cttctatttc atgtttatca aaaaggacaa tatcatgtgc ttgaatcatt ttttatgcat
    48121 cccattcttc gtccattttg gcacggactt tatttgtttc gacacgtccg aatgttttgg
    48181 ctgtttcgtt tgtgtatcga tttgtcagca tcgtgtcatt tgtattgcgg acatcttgaa
    48241 tggcttcatt gattgcttcg atgactttgc tgacattttg ttctgaagca gcttggtcgg
    48301 tgatatcttc taggaaatga tagatcgctc ctagttttgt ataattattg atatcgtaag
    48361 cgatagctgg aactttttca gcagctgctt cgatttcagc aaccgaacgt tttgtcacac
    48421 gtgcagcact ttctttagac atcgcttgga cggtaatttc tttattgttt aaagcaatca
    48481 agcggcttga ttgtaatcca tgtgctaaaa atgcacctga aatagcattt cctgcaatca
    48541 atccaataac tggatatcca gcttgacgga attttgcata agcagcagca ctattagcca
    48601 aagccacatg gataccgatt aattcttcgt tatagccgta tgcttggctt ggtacatcga
    48661 tcacaacgat gattggccgt ttcacttttt tatctttatc ttcttcgtaa acgtgatcca
    48721 aaatttgtcc catgcggaaa ccttcaacta aaccaacttc accgtttcgt acacggtaca
    48781 tcgggttttt aggatcaggt acgattgata tcacgatggt ttcttctatg aatttacttg
    48841 atttaccata atacacagta ctaatagaag aagttggatt tttcatgcca gttagtgccg
    48901 caaaccaatc gtaaccaaca cttgctgcag ctggttcatt tccttctgct acttctggta
    48961 agtcaattga ttttgcttga tggttagcaa ttgcttcatt gtaatcgtca atggccattg
    49021 gttttgaaag atctaaagaa tcaagtaatg ataaataaaa tgctgagctt tcactgcgat
    49081 gcgtgtcttt tcgttcgtcc attgctgcga tgacagcagc tttgattgct tctacactat
    49141 cagtcactaa ttcatcaatg atttttgact ctacccgttg acgagatcct aatgtattcc
    49201 aaataaagtc tttgtcacta gaatcaaatt ctcgaacgcc tgcttcttgt tcaataactt
    49261 ctggtccatt caaaccgact ctcgcttttt ttgttgcgat taaataactc attaatgctg
    49321 aagtaatcga cataccgcca aatgatccta cacgtcctgg aatcaaaccg atgactggta
    49381 catattgttt tagagcaatg attacatttc cgatttctga aatagataac agtccgtagt
    49441 ttgcttcttg caaacgaaca ccacctgtat ctagtacaat gactggaaag atctcatttc
    49501 cttttttatt ttcttctaac acatgttcaa gtgcagcaac gatttttgca ccggaaactt
    49561 caccgatacc gccaccttgg aattttcctt ccatagcgat tactactgct tgtttgccat
    49621 tgatcgtgcc acggctgaca acgatcccgt catcactttc agggacgatt ccttgtgctg
    49681 gcaaacttgg cgcgatgatt tgatcgaatg gatcaatcaa ttcacgtcca ttgtcaagta
    49741 gagcgttcac tcgttctctg gcgtttaatt ctacaaaact atttttcatc tgaaagttcc
    49801 tccattgctt gcgtcaatcg taggtacaca actcctggtg tagcaccgaa gtcattgatc
    49861 acgatatctg ctgaaattgg ataacgatca aaaaatctcg ttaatacatt gttccatact
    49921 tcgccgaagc catcgcttcc tgtacaaatt tttacagtag attctgtttt ttctgttgga
    49981 aacatcaaga tttccaaatc tcctgaaccg accactccta catggatttt cttattgatc
    50041 ggctgtttcg ttggatattt ataagctaat tgttccatgg atctctcccc tttttgtaaa
    50101 acagagcata cacgttctgc ttattcgtct ttcttcttcc atctgggtaa atggcgacaa
    50161 aaggtttttc tcgccaattt tatgaccaag ttctgaattt cattggtggt tcatacaggc
    50221 catctgacca ttcgatcaaa tcatcgattg tttgtgcagc taataatgaa cgtttcgctt
    50281 cactgcgttt gatacctaga tcttctggta atttcacgat gccggcttca cgcaagctat
    50341 ctagtgattc tttcgtgcta gtcattccaa gaggtgtaac accgccaatt gctgcaattg
    50401 ctgcttgacg ttcttctttt gtttttgctt tgtacaaata agcaattcct tcttctgtca
    50461 cgatatgggt agtatcttcg ctatagatca tcactggtgc attggctaag cctgcctgtt
    50521 tttgaacaac taatgcatct aattcttcga caaagactgg ttttttattt gaaccatatg
    50581 tttcaaccat ttgaacaact aatttttgtc ctttacctaa tggatcatca ttgtcgcgaa
    50641 gtgattgcca tgctggtgta gaatgacgac gaccgccagg attatgtccc atgttaggcg
    50701 ctccaccaaa accagataaa cgtccttttg ttacggtaga agaatttcct tcgtaatcta
    50761 attgcaaggt tgaaccgata aacatatcga ttgcatattg tccagctgct tgtgataatg
    50821 ttcggttact gcgcatcgtg ccgtctttac cgacgaaaaa gacatctggg cgagcagcaa
    50881 tatatttttc cataccgact tcaccaccga aactatggat actttcaacc caacctgatt
    50941 cgattgctgg aatcatcgtt ggatgtgggt tcaataccca gttcttcgcg attttccctt
    51001 ttaaccctag tgattcacca taagttggca gtaataattc gatagctgct gtattgaatc
    51061 caataccgtg attcaaagat tgtacgccgt gctctgcgta gattcctttg attgccatca
    51121 ttgccattag gatttgcaat tctgtgatgt tttgaggatc tcgtgtgaat aaagcttcta
    51181 attgataagg ttcatctgct tttacgacaa cgtctaccca gtcaccaggg atatctacac
    51241 gtggtaaaga atcgacaact tcattgactt gaacgatcac gatgccatct ttgaaagctg
    51301 cagcttcgac gattgttggt gtttcttccg tattgaaacc tgtatacaag ttgccgtctt
    51361 tatctgcttt atctgcaact actaagacaa cgtttggtat caaatcgatg aataagcgtc
    51421 cataaagttc caaataagta tggatatcac ctaatttcat tgtgccgtct tcaatcattt
    51481 gggcgacacg caagctttgt gcacctgcat aagagaaatc gatcttgctt gcgattcctt
    51541 tttcaaaaat attcaaatgt tctggtcgtg agatactaga catgatcaaa tgcagatcat
    51601 tcactttttc tgcatctact ttttcaagac tttctgaaag aaaacttgct tgtttttgat
    51661 tgtcgccttc taatacaact ttgtcgccgg gagcaatcag tttttccatt acttctacga
    51721 ttttttcagt ttctacatac ttgccattga gcaagttgct gattttttcc aatcgctgcg
    51781 cttttttttc gcgtttagaa gcccagttgc ggtttggttt ttccataatt agtcatctcc
    51841 atttttgata tagaaacgtg gcgtcatcaa taccatgttg gtctagattg atcgtacgga
    51901 tccctttatc tacaccaaat tttgtatgaa tcagctttgt caaagtattg ccggggggaa
    51961 attcaatcgt cagatccatt cctaattctt ttgccaagtc catcatttgg ttccactgca
    52021 cagggaaagc tagattttcc aacaaatctt tacgaatctc ttctgcgtcc tgagtggctc
    52081 ttccatccgt attttttata tacaaacatc tggcatcaga tagatggaca gactgcatat
    52141 acggcttgaa tttcttgacc gtatcattca tcaagagaca gtgagatggc acagggactt
    52201 tcagcagctt cgctgtttgc gcttggtgcc gtttggcttc tgccaacgta acggataaac
    52261 cttcgatact cccagataac gtatgttgca tcggacaatt ttcatttgac agataaactg
    52321 gatggtcagg atcaaatgtt ttggctacca tttcctctgc ttcgcttctc gtcacaccta
    52381 ctactacgcc cattgcataa ccgcttggat atgcttcttt catcaagact gcgcggtgat
    52441 agactagttc cgctgcattc tcaaatgtca aagatcgaca agcaacagct gcacaaaaag
    52501 caccgatcga atgtcctgct gcaatatcag gctgaatacc atttttttcc atttcatcta
    52561 aataaaatac tgcttttgtc agtaatgcta gctggataaa aaccgtatct tgatagtttt
    52621 cttctttatc gactaaccga taccccgtca cattttctaa acgttccatg tacatttcag
    52681 ggatatccgc taacatccct aacttctgac tgccttgtcc aggaaataag aaacctgtac
    52741 tcatgagcct cgcctcctca gcacttactt tttccactgc tatcttagtc aatttctgct
    52801 ataataaaaa atgccaaaaa aagaaaaaac cataacaaaa aggtatggat gggatggata
    52861 attatgaaat tgcttcatct gcagtatttt aaaaaagtat tcgaaacaaa aaatgttacc
    52921 caagcagcca aagaattatt tatttctcaa cctgccttga gccgtgcgat caaacatttg
    52981 gaaaacgaat taggtgtccc tttgttttac catagcggca gaaacattga gacgaccgtt
    53041 tatgcagaag aattttatcc atatgcagtc aagacattat acacattaga agaaggaatc
    53101 aattattgtc ttcggtcaat gagaagatcg ttacttccgt catattgtat ttagaggtag
    53161 cttctgtttc tattccgaac ttagtccgaa tttttatgga aaagcatcct gatatccaat
    53221 taacgatcat gcaacatcat ttaccagatg atattaccga tcctgtactg tacatcactt
    53281 cggagatgaa gcctggcatg accaatattc ctatcatgaa agaacctgtt tatgtcgcca
    53341 ttcctaagaa acatcctctt gcgaaaaaag aacatctgca attagaagat atccagcgca
    53401 cccccatctt gatgctgtca aagagcaacg ccttccgtca tacactggat gcagcacttg
    53461 aagcgaaaag tatcgaattg acgatcagtt cgacgacaga tgatcctgcg acattacgtt
    53521 ctattgtcaa gcaaggatta gctgttagct ttttcccaaa agtttcttgg tcttacgata
    53581 aaaatgatcc tttcgtcttg cgggaaatca aagatttgcc cttgacacga acaatttatt
    53641 tatcttctag cttcacagaa gatcatcctt tgatcaaaat gattgcaaaa acgatgcggg
    53701 aattttattt aggaaaataa aaaaaaaaga gctgggacaa aattcttgtc gcagctcttt
    53761 tttcgaacaa acggtgttcc agaagtaatt tcttagaaaa taagccgaaa tttcccaaaa
    53821 atttgagaag caattttcgt aaattccttc ttatttcttg aagctaaaca tttctgtcac
    53881 aacctcttct ctattgtcct agacattcat tgcgttgatt taaaacttcc atcaatcaaa
    53941 tctgctagtt catctagctt ttctatttta cgtagatcaa tagagatcca atgttcttta
    54001 ttcatgtgat aggctggaaa atacccctct tttcctcgca aaattccgcc aagttcagga
    54061 tcgaccttca aatcaagaat attgatgatc ctatggtcac ttgcatcatc atagattttt
    54121 tcttctggaa cagacatcag gaccccaaac cattttttgc ttttttgatg tctgaatacg
    54181 caataatcag gaaatttttt aaaaatatat tctggagaca aattgtatgt ttcattgatg
    54241 tgatcgatta gttctttttt cgttagcatc tgcttccatc tctttcttat tgattcgctt
    54301 tattcaaaga taccacgtaa gaatagaaaa atgaatacaa gtagctgcta atcaaatact
    54361 tcgatatttt tcttttttat caactcttaa atgttcaatc cgcctgtgtt ggtcttgcta
    54421 tcatctacta ttaaaatgtt gattattgtt tgcccaccta ttttttgatt ttagtggtat
    54481 catttttcaa gttcatgatg aaattcttat catcagtaaa tgctccagaa aaataaacaa
    54541 tgattgaaaa atcagttgaa acttgaccac cgtttatttt ctccgtattc ttagaataga
    54601 agggagtctg attgatgtat tcactaatga aaaaaatcat taccgaaaaa gatattcgac
    54661 gtcatgtttc tttagtagaa caattgctga atcatacaaa gataaccgta aatgaattag
    54721 ctgaaatcat agggacaacc gaacgaacga tttattctga ccttcaatct attcgctctc
    54781 aactgcctga aggctgggac atcattagcg atcaagcagg gattagtttg caaaaccagc
    54841 aaaatttatt agcaaatgac ctttgggaga tttttttcaa acaatcagtc agtgtcgaac
    54901 tattgaaaag tctgcttttt acaaaaaaag ttgctgtccc tgattttcta gccgaccatg
    54961 ggctttctta tgggacattg aaaagacatg tgactaaaat caatcagcgt ttagcaagtt
    55021 acgacttgca gatcgatttg acgaaatata ccgcttctat tcttgggaaa gagcgtgcga
    55081 ttcgcacttt ctatcatcga ctgctgattc cttttactca taacaactat tttttcgaag
    55141 attattcgat tcatgaatcc cattattttc aatttttaaa aacaataaat cgaacggagc
    55201 tagcggttga aacagaagag atttttggaa catgttggtt ctttatcaat acgatccgta
    55261 taaaagcgaa ttgccgatta gattcttcta tccatatcaa cagtacactc tcctccctgt
    55321 atgactctgc attgaaaaaa ttgtatttaa cggaaggtat ttatctcaaa gacactgagc
    55381 tttcttttgc ttctttctgc tttctggaaa gttggaatta caacaataac tatggacaag
    55441 aaaccgcttg ctgcctgcat cattctccat ttttcgaacg cctcgaaacg tttgtagagg
    55501 aactagcttc agagctgtct cttgatcaac taaaaaaaca tctctaactg ataacttggc
    55561 tcttctcgta ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata
    55621 ccattttttt tagaaacata tgatcagcaa tatgcacatt tgtctcaaca gaaagagaat
    55681 ttactgaacg atctaaaaaa agaaatggaa atcaatgaac cgaatttctt ccttcaactc
    55741 ttatctttac tgatccaaca aacgattttt tctatcaaac cgaatttgtt caatgtctat
    55801 tttattttcc aaggagaacc ttcatggaag gcattctttc agcaggaatt aaaagattac
    55861 ctgggaaaac gtgtctgctt cttacctatc gaactaaccg atctttccac tatttctttc
    55921 gaaaaaacag atatcttgat ttccaatttc ccgctggatt ctctagatat ccctatcgta
    55981 tacatttctt ctattcctac gaaaaatgag ctcaatcgtt tgactgaact cactttcaaa
    56041 acttttttat agtaacggct agcgactgta ttctctagct gttttttctt ttttgcgaaa
    56101 cagtttattc atgatccctt tttctatttc tacaactaag aatagaagaa tcacatttgc
    56161 tccaatcaat aactgttgtg tcatcgttaa acttactgta ccgatcagct gctggccgac
    56221 tggtagaaaa ataaccatcc cttgcaataa agcaaggata cctaatgaaa gaaaaagcgc
    56281 tttgttttgc aaaagaccct ggttcaatga cggcttggac aactctctgc aattgatcat
    56341 gtaaacagct tgagctaaga cgatattttg tagaagaatg gtttgctgta agctttgccc
    56401 ttcaaactgc atagccaata aataggcagg aaccatgatc agtagggaga catagatgat
    56461 ccgaaacaaa tgataaccag ataggattcc ttgctttgga tctcttggtg ggcgcttcat
    56521 gatttctgga tctggcgact cgaaccctag tgcataagat aaagtgattg tcgttaccat
    56581 attgacccat aaaatctgta ctgggaacaa aggtagcggg cgattcagca taagcgccca
    56641 aatcaagatc aatccttgag ctaatgcagt tggcaagaag aaattgatgg ttttttgcaa
    56701 attatccaaa atccgccggc cctcttttac ggcttttgca atcgtatgga aattatcatc
    56761 tcctaaaacc atatcagctg cctgtttgct tacttcacta cctttgattc tcatagcaat
    56821 tcctacatcg gatcttttca aagcaggtgc atcatttacg ccgtctccgg tcattccaac
    56881 gatttatcca tttttctgca aagctttaac aattcgcagt ttatgttctg gcgtcgttcg
    56941 agcaaagaca tcgactattg gcacttgctg aatcaactct tcatcagaca taagatccaa
    57001 atcgatctct tctaaaactt tggcagtatg ttttaagcca atttgttccg caatcgcttt
    57061 tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag atccctgctt gttgacattc
    57121 ctttacagct ttgactgcac tttcttttgg cggatcgatc aatccggcga tccctgcaaa
    57181 agttaattgg gttaaagtct catgcgtgag ttcctgtttc gaatctactt ttttataagc
    57241 aaatcctaaa acacgctgac cttttctcgc aaattctgca gctgtctgac tccatgcttc
    57301 ttgttctgca gaagaaagag ttgatttagc aaataatact tctgatgcac ctttgacgta
    57361 aatcaccgct cctttttcct ccgcatgcat cgttgccata tatttgtatt cagaactgaa
    57421 cggaatcttt gcttctattt ttttcaaagg cagattggct gtttctgtgt gtcgaatcag
    57481 cgcaacttct gtcggattac cacttaaatt ttctgttttc tgttcttctt ttgtttttaa
    57541 ttcctgacag tttttcataa tggacaacac gcaggcttct tcttttgcag ctacatccac
    57601 aactgtcatt tcattttttg tcaatgttcc tgtcttatct gaacagatca ctgtcataga
    57661 acctaatgtt tctaccgaag gaagtccttt gatgatcgcc ttttcttttg ccatttcatg
    57721 tactccaacg gataggatca tggtcaaaac agctggcaat ccttcgggta ccattgacac
    57781 caccaaagca atcatagccg aaagaaggat atgccaatcc ataccatatc gaaatgtcgt
    57841 aaaaataact aagaataggc tcaaacaaag aatgccttga aaaatttgtt tgtttaatcg
    57901 gttgattttc ttaatcagag gcgtcgtttg ttgttcgacc tattgcaatg cttgattgat
    57961 tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca ccgactgcag aaccagattg
    58021 tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg atcgattctt cttctgtcaa
    58081 aacggcatga cttttttcag atgcttctga ttctcccgtc aaaatcgatt cttccactaa
    58141 taaatcatgt gcttcgatta atcggatatc tgctggaacg acatctccgg cacgtaagac
    58201 aatgacatct ccttttacca gttctttcgc agggattttt tcttgcatcc cgtttgatag
    58261 tactatcgct tcttgtccca tcagctgctt caaaccattc aatgattctt ccgcttttct
    58321 ttcttgccag taactaacga gtccattgac gatcactacg aataaaataa tacttccttc
    58381 aatatattca ctagaagcaa atttcaaaaa agaggcaatg atcaacacga tcattagcag
    58441 atcagtaaag tgctttgcta tttttttcca tcgttttgtc tgctgccttg cttttatttc
    58501 attatatcct tgatcagtca ggcgtttctg tcgttcttct tgcgacaagc cttctgttga
    58561 tgtttgatag tttttcaata catcttctac cgtcaatctg taccagttca tcctatcccc
    58621 tcctgtttaa ttgatactgt tattatacgc agggaagttt tttcaggcgt atcgattatt
    58681 tcaggggggt ctgaaataat tcgttttgca gaatgtatct gggcctaact gatatttcta
    58741 ttgcttattc ttttatatac aaaatttaag aatacaaacc agataaccaa taaaaaagga
    58801 ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa ttgaagtaaa aaacaccaac
    58861 aaagcaagat ctttatatct ttgtgtagtg ttccgtttct tttctttaat aaattgaaca
    58921 tgtgtacaca agatcgaaga ccacacaaat agaaaacaac tcgttgtcat gcctgccaaa
    58981 agctgaaaaa acaatcgact atcagctaca aaaaaatgta gacaaagacc ggcaaaaaat
    59041 aacattcctg taattaaaat accgtgtaaa ggtacgcctt tcttagaaag cgcacaaaac
    59101 caatggggca aacttttctc ttttgctgtt tgatataaaa tcctgctagt actgtagact
    59161 gcactatttg ctgaggacaa agaagcaacc aaaatcatta gattgacaat tgctgctccg
    59221 ccttggcaat ggatcatttg cagcatagtc actaaaggac tttcttttaa atctaaacaa
    59281 tgccatggaa caacaaataa aatcgacaaa atcgttccta tatagaaaaa gagaatacgc
    59341 caaggcagac agttgattgc ttttttcaaa ttcttttgcg gatcacttgt ctcgccaacg
    59401 gttaacccga tcatttcaat tccagcaaaa ctgaataaaa ccataggaaa tgagactacc
    59461 attccagcaa agccaaacgg tagtaattca tcgatctgtc caaaattttc tttgattccg
    59521 atagactggg tatgaaaatc tgagacactc aatcctatac ccaataagat aaaaaggaaa
    59581 atccccagga tcttgatgat tgagaaaaaa gattccaatt caccaaacca cttgactgca
    59641 acaaggttga ctgccattaa gatcattagt gcgctacctc cgcttatcaa agctggtaca
    59701 tgtacaaacc agaaatggat atacaatcct attgctgcta attccgcaag tccagctcca
    59761 agccagcaaa gccaataagt ccaattgaca aaaaatgccc actttttgcc taaataatag
    59821 tcaacgaaat cactgataga gttaaatgta ctatttgcta aaaataaatc acccatggcc
    59881 cgcatcaaac tataaatagc caaaccgcat atcaaataaa cgagaataac agctggccct
    59941 gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg ttccaattgc gccacctaat
    60001 gcgattaact ggatatttcg attagttaga gtccgtttca ttgccggttt ttccatcaag
    60061 ttcacctttt tctttttcaa gcagcatggc gacttcctgt tcccaagaag gtttctttat
    60121 tttttcaata cagagtggta ctacaaacaa aatgacactg atggctgtga ctgcaattag
    60181 attgatccat gttccagctt tcatcgtcag tccgacagaa aatccgattg ccgcaaataa
    60241 aacaataacg acagtccaaa ctccaagatt gctctttttc tctttttttc caattctaaa
    60301 tggtcgattc aactttgcat gtgtataacg caatctcaaa actgcgatcc ccatgatgac
    60361 atagacgata cagtagataa cagtagttga atttacgagc atcaaaaatg cttgattgac
    60421 gccaggaatc agcaaataaa aaagtgcaaa gattgagata atgacagact gagtcaaaat
    60481 aacggatttg gatacgtcaa attgattcgt cttccaaaaa ttgaattttg gtggatacgc
    60541 accttttcta gcactttctg tcactgtctt tgctggtcct gaggcccaag cagataattg
    60601 gactgctaca ccaatgaaga caagtaaact aaacagattc acaataagat gtggcaaacc
    60661 aagaatttgg caataaatcg aaatagattg cgcgatatta ttcaattcca tttgtccttt
    60721 tggtacgaca tttgctacaa ctaaagcatt caaaatattg acgaaaaata taaaaattaa
    60781 cgcggcaaat actccgcgaa tatacgtttt aactggattt ttcaatcgag tgatatacac
    60841 agaggacatt tcgataccag taaaaataaa catgatcggc gcaaaataaa caaatgagga
    60901 tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg
    60961 tacgatccca actttgatcc acgcagcaaa ccctaataag agcatcatcg tcaaaggcac
    61021 gtacaatcct aaccaaacac cgacttttcc gccgattttt gccatatcaa atttcaaatt
    61081 caaaaaagtg atgatccaat aaacgactaa aataacaact aatgtaaatt tgctgttcaa
    61141 accaagcggc acattatcga taatattacc aaacaacgga gcaagtgctg aagcgaccat
    61201 caccatccca ggaaacattt ggacccataa cagccatgag acaacgaacc cccatttttt
    61261 tcctagagca ttcgataccc aaagttctgg accacctttt ttgggaaaca ttccggcaaa
    61321 ttcaccagag atcaatgaga ttggaaaagc gtacaataat actgcaacca gcatgtaaaa
    61381 aatcatcgtc cagctcgttg ctgctacatc tggaatattt cgtacacttg ctacaagggc
    61441 gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt gatgaaccag tatctttcat
    61501 ctttattcct tctttcaata gcgacatatg tttttgtttt cttttatttt tgttcaagaa
    61561 cagaaatgac aaggtttcat cgattcagta caaaacccag tatcaccgaa acatccattg
    61621 aacgatctta gtgaactggg tttgatacat gaaacgaacg atccaactta tagaacgttt
    61681 tataaaaaag aatatcttgg cgaggaatat atgaaaaaca gggggaaata caattgtttt
    61741 gatgaagcaa ataattgtct gttctccata tgtctcttgc tattctttca cacaaaaatc
    61801 agttctattg gatgatttcg tttagtttct tttccattgc tttggcgatt tcttttgtat
    61861 aaaaattgaa aattcgatca tcattcaaat aaggagtcat gatagccgca cgtagcagag
    61921 tgattttctg ttcttttttc cattcttctt tcgttagacc cattctttca acaaaacgaa
    61981 tcggagaatc accgtaatct tcttgtgtaa atgtagtatg agaagtaata aaccgttcac
    62041 cataaacatc gccatccata taagaagacc gatcgaacat tttttcattt aattcattga
    62101 tagcgttcaa atctgtacag ccttgttctt tgaataccca attgaccata ttaaaatcag
    62161 gatgattcaa tggatagact tcgattgttt ttcctttaac agtaaaagtc atatgatcga
    62221 gaaattctct gaatctttga gctgcctcga ttgaagcccc aatcaattga ccgtatccgg
    62281 tcacattcaa tggcaataca cgatgtgttg tccagactgc ggcggcggtt gctccagctt
    62341 ttgatccttc caaaatataa gctcctaaca tatctggagc ctttactgat ttttcaaaca
    62401 catatggcgc gaaataagaa atgatatttc tcatattttt atgtttgatt acgattcctc
    62461 cagcagcata agggacatag cccatcttat gaggatctat cgtgacagaa tccgcttctg
    62521 aaatagctct gaacccttca tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat
    62581 gatgttcttc aaagaattca gctaaggaag catacggcac aaattctcct gcttcgttta
    62641 gaaacagtga acgcgcatag ccgccatatg cggcatcaac atgtaaatag aaatagatac
    62701 cttcatcttt taacctttct cttaattgga cgattttatc tacactgtct acttgacctt
    62761 cttctgtcgt tcctacgaca gcaaccacac ctagaattgg gattttttgt tcagctaatt
    62821 cacgaattgt tttttccaaa gcattaatat ccattcgata atcctcttga actgggatag
    62881 caaccatctg atctaagccg actccgcaaa tatctaacgc cttcatccaa gaatagtgtt
    62941 ttgtttgagg gaccagccat ttccctaatc tttggatatt ttttccgcta cgggaagaag
    63001 ctgctttgac ctcatccatt tcttcgtccg taaagctttc ggtcatttcc aaaatttctt
    63061 caacagataa attcaaaagt tcccactcag acattttttt cactttttca ggcagcactt
    63121 ccttgacagc taaaggaatc gatttgatgc agcgggcgta ccataatcct tcaagattag
    63181 caatagaacc atctgctgca atgtgtcccc atccgtctgt catattgaat aatgaagcaa
    63241 aatcctgtcc tacttctgct tccatttgcg aagttgccat agaagattcc aatgcaacat
    63301 tatttgggtt ccacagcatc gcatagttgt aagctaataa agcaggcatc aatgtttcag
    63361 cattcatttg tccccagtaa cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc
    63421 gctgactgac ttcatccaag acctctatca tatggtcgcg ggtggctaga taatctggag
    63481 tgagcttatc tccttcgcta attgcaggca tatctgaagg aatataattt tctctccatc
    63541 caagatgttc atctaccatc ttgttcaaca acattttata aactggtcca ttttcagctt
    63601 tgtcgcctat aaagacggcc ttgatatcca tatctttcat ttttcttccc tccatatttt
    63661 gttggtcaat gtctcgcaga tacatgtgtt tatctgtaac acctcttcac tatagcgcca
    63721 aaaatgagaa atttttttta taacatcaaa tccacgtaga tttttacaaa caaaaataaa
    63781 taaagtcgga tttttctgat aattaaacgc aaaaaaacga acattttttc aataaatcag
    63841 tgttctaaaa tagataacct aaaaaaagcg aacaaacaaa taaaatgact gtccttcatt
    63901 cactaaacat atttttattt ttttatattg ttaaaaattc acaatttatt ttttaataga
    63961 attatctgtt ttccaaattt tataacataa aaaacctttt ttaacggcat ttatatgctt
    64021 tttcagaaat taactgatga tttcaaaaag aaaacatgtc ataatatttc ccatattgtg
    64081 caaaaaaata caaggcggag gctctactat gattgaagaa tatattgaga aaaatatttt
    64141 aagacagctg tttttatgtg ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct
    64201 ctccaatctc ctgcatgttt gcaaaaccac tttgctcaat gatatcaata acataaaaaa
    64261 agaatttgaa gaacagatcg cttatacaca tcgggaaaag gaccgttaca ctttatattt
    64321 ttcagaacat atacctcgat gcaagatcat gcagcaagtg tctcaaaatt cgttgttttt
    64381 aaaaacttgt ctgctttatt tggaagaaga tgaacctgat tatcttcaat taactgaatg
    64441 tgaattcatc tctgtcagca aagcttattc attaaaaaaa caagtattag cttattttaa
    64501 tgactgcggg atcgaaatcg accgctactc ccctcgactc actgaaatgg aaagacgtct
    64561 gctgcttctt aatgtgtcgt atcgtttagg cggttttaat tcttgggagc tacctgaatc
    64621 gttttttgaa cgtaccgatc gtttcattga atccgtaacg gaaaatagtg gcagatttta
    64681 tgataaagaa aataaagaaa tcttatcgat tggttttgct atcagtttcc tacgccagca
    64741 agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac gtcctattta
    64801 caattatgtc gaatcagcat gggaaaacac tgatttccaa acctattata aaaaagaaga
    64861 attcgccttt attttgacac tattcaatct ttgtaattat ggtttccatt cttatcagtt
    64921 gattgcggaa gactttcaac agctacacca agtgtttatt gacaatactc ccgaaataaa
    64981 agaactagtt gctacctttg aatctcactt caatcaagaa ttattcggaa accaaccgtt
    65041 cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat aactatcaat tattcatgcc
    65101 agaaaaattt tatcttttaa atgaggaaca gacaaattta tataaagaag tccagacaat
    65161 tttttccagc tggtccagcc agttacctta tgatctacgg ttgaatccta attgcatgcg
    65221 ggcttttgtg atcgaattga gcggaattct ccgtctgaca aaagaacatc tgactatcta
    65281 tatcgtcaca aattcggatg ttcattatct gatttatcgg gaagccttgg aagctgtaac
    65341 tactttcgat tttcaagttg caccaacaat ttattcaagt atatctgata taaagaagta
    65401 tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca ttatatacac cagatgcggt
    65461 ccaatatgaa aatattattc ctatttcgat caatacgatc gatcgagcta tcatttcagc
    65521 tgtgcaaaac aaataagggg ctgtgacaaa aatcatgtca cagcctctta tttgttgaag
    65581 atgcccattt ctgtaacaat ctcttgatac acgtcaggct cacaacctca cttattttgg
    65641 acatctacgg gtccgatctc tttatttaaa aaataagaaa tgatggttcg gataactacc
    65701 aacgccgcta gttggaaaat atcctgcaag gtaggtttga taatcgattc gatgatccca
    65761 gctgaaacaa gaatttccaa acttagaagt atatagcctc ccaacatctt tttaatagca
    65821 ttgttctcgc tgactcggtc gttttgcact tttgtatgga caaataaata tttcaaaaaa
    65881 tccttgattg atagacaaac accccaaacc aataaacaga tagcaaagat atcaagtccc
    65941 agtacaagtt tttcaaaata tggaagaaga aactgcatca actctgtcgt catcctacct
    66001 ctcctcttct ttgtttttct ttgtcttcta tttcctgatt caagaaataa gaaatgactg
    66061 ttcgaatgat aataataagt gcaagcttca aaatatctgt ccaagtcggc ttgataactg
    66121 actcgatgat atcagcaacg actagtactt ccaagcttaa caaaatataa cttgctagca
    66181 gttttcttgt atctgcatta cgagtattac gttctgcata gtttttcttg acatgagacc
    66241 aatcaaaaag tttgaataga acaagaacga tcccgacaat taagataccg atagaacaaa
    66301 tatttaaaat agcaatgatc cattctagaa attcaattaa atttgagcga aacatatacg
    66361 ctacctactt tcttttaatt tgttcctatt cttttattgt aaaagaaagt taccttgttc
    66421 gtctatttaa aagttgttct aatgataacg gttcgcttaa atttttcttt ttcactctcc
    66481 catttttctc ccctcatcca taaatcgtgg aagtatccaa aacaacgtag caagttaaaa
    66541 taaggcttag tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt ttttgtaccc
    66601 aaacttctaa aaatagaacc atacacctcc tcaaatcttt tcttcttacc taccctattt
    66661 ttatttacag gaaaatcaga cacttcttct tttttgtcaa aatttccttt ttcttctcgt
    66721 cctatcattt ggcattctaa taaaaacgaa ctatccttgg atatagaaag agaaaacaag
    66781 gaggcgtttt tcatgagtaa aaaattaatc aaagtcggca tcggtttagg tttgcttgcc
    66841 cttggcgctg catatctagg aaaaaagaca ggactattcg aagatgacag tcatttatat
    66901 gatgaattcg aatcaatcta gcatcctatt tcgttgaatc actacggaca tacttgaaag
    66961 acattggagg aatcacaatg agaaaaagta aatcaataat gattggtgcc ggtattgcca
    67021 atatggcagc ggctgtttat ttgattcaag aaggcaaatg gaaaggggat cagatcacct
    67081 tttattcatt ggatgatcac ggttcaaatg atggtgcacc gaccgacact gtagtggatg
    67141 aatattggaa taaaaaccat ccattagaaa accaaaaagg ctatattgct cgaggcggtc
    67201 gcatgcttaa ttaccgtacc tatgtagatt tgatggatct gttaagtcga ataccttctg
    67261 ttactgagcc agggatgact gctgaagaag atacgagaga ttttgatgca aaacaccgaa
    67321 cctttgacaa agcacgtttg atggaaggtg gcaaaggaat cattgatggc ggaaaactgg
    67381 gattcaataa taaagaccgt attcttctga ctaaactgat tgctatgcct gattcagaag
    67441 aagaaaaact agacaatatc actatcgaag aatatttcaa agatgatcca cacttttttg
    67501 aaacaaactt ctggtttatg tgggaaacaa catttgcttt cagaacacgt agttctgctc
    67561 aagaattgcg tcgctatatg catcaaatga tttatgaatt cactcaaatc gaacatctag
    67621 tgggcgtcaa tcgaacacgt tacaatcaat acgaaagtat tatgcttcct ttgatcgagt
    67681 acttgaaaga acaaggatgc aaaatcattt tgaaccgccg tgtcactgac tgggaattca
    67741 aagaaacacc gatgcaagat gaaatcactg tgactggttt gaaaatgacc aatacactga
    67801 ctcaagaaga agaacacgtt gtggtcgatg aagatacagc agttattttc actaatgggt
    67861 ccattacaga ttcagctact ttaggagatt acgaaacacc agctgtagaa aatatggatt
    67921 atggtgcagc ttctagtctc tggaaaaaag ccagtgaacg tttctacaac ttgggaaatc
    67981 ctgacaaatt ctttgctgat cgagatgcaa gcgaatgggt cagctttacc ctgacgacga
    68041 aaaaccattt gttgttgaat gaaatcaccc gtatcacgac tcaagaacca ggaaatgcct
    68101 tgaattcatt tatttcaacc acaccgatca cgccgttagg ccaaaaagat gtcaatatgt
    68161 ctatcgtcgt gcatcatcaa ccgcatttta cgtctcagaa atcaaatgaa acagttattt
    68221 ggggttattt cctttatcca agacgacgtg gcgaatttgt ggataaacag tatattaaaa
    68281 tgaatggaaa agaaatgcta gaagaactga tcggacaact ttcaaaagta gatcctggac
    68341 cagtgaatat tcgtgaaaaa gaaacagaaa ttttcgacag cgtgataaat aatatccctg
    68401 tctatatgcc ttatgcttcc gcgttgttca ataatcgtgc caaaagcgat cggccaaaag
    68461 tcattcctaa acattcgacc aatctggcct ttacaggaga atttgtcgag cagccttatc
    68521 agatgatctt tactgaacaa agtgctgtac gttctggaga aatcgctgct ttccattttg
    68581 cgggtatccc tatgtcccgt cttgtcaaaa caccgcgtta cgacaaagat attcctactc
    68641 tagctcgtgc cgcaaaaaaa atgtttgaat aaccaagagg ttgtgaaaaa gctgacctgc
    68701 atcaagtaat aagaacaacc aggctaaaac agcttcttat gtttttgatt tggttggact
    68761 tattaccgca gatgcaagct tttgaacacc gtttaccaag aggttgtgaa aaagctgacc
    68821 tgcatcaagt aataagaaca cccaagctaa aacagcttct tatgtttttg acttggttgg
    68881 acttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg
    68941 acctgcatca agtaataaga acacccaagc taaaacagct tcttatgttt ttgacttggt
    69001 tagacttatt accgcagatg caagcttttg aacaccgttt accaagaagt tatgaaaaag
    69061 gcgtttagtt tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata
    69121 aatttgactt aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct
    69181 gtttcttttt gaaaacaacc attttttcaa cgtttactct cttgccaatc caacatcatc
    69241 taacggatac aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc
    69301 cgaacacaaa tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc
    69361 aaaaaggtaa gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt
    69421 tatgaatttg aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat
    69481 ggaaatcaga aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat
    69541 cacagcaaca gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt
    69601 ggaaaaaggg tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca
    69661 agttcaaaca ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca
    69721 ttgtcatttc tgcctgaatc acatggaaag ccaatgcgat gaatcaaatc cagatcagtt
    69781 atatggcggg cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga
    69841 atatctaaga gttccttatg cagactctac ttcatttgtt gtaccagata atgttcttcc
    69901 tgatgaaaaa gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa
    69961 cgcaggagta aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt
    70021 cgctcaaaaa ttcgctgtta tggctggagc agagcgcgtg atcgcagtag atccagtaca
    70081 gcaccgtcta gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac
    70141 ggcacaagct ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga
    70201 ttgtgtagga atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt
    70261 acaaagcggc acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac
    70321 agtccaagta actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt
    70381 tatgcgggat atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat
    70441 ttacgaaatg atcgcagaag gactattcga tcccacacaa atcattactc attcgctgcc
    70501 actatcagaa gcagcacaag catatgaaat ctttgacaaa aaagaagata aaaatataaa
    70561 agttgttttg aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat
    70621 caaacgaaat acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg
    70681 tcgcttccgc ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt
    70741 aaccgggatt ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata
    70801 tggcacctat ttttatttgg ctggtgctat tttcttgaca attcgtccta ttctcagaat
    70861 cgtaaggaac atttttattt acgacgaaaa aaaacaatga agagaacaag caaaaaaaag
    70921 aggcatgaga tttgaaaaaa tctcatgcct cttttttaac cctatcgtat cactttgata
    70981 cagacgagcg gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc actcgtatcg
    71041 ttcataaaac gtggtatgat ctgtaattaa ataaagtttt tcatacccaa gttgatgcat
    71101 tttttctaat ttccgctaat atccgcttgg ctatcccctg cttacgcttt tccggttcaa
    71161 caaacagggc acaaagattt ggtgtcagat cgggacggtt atggaagtcg ttttcaatga
    71221 cgcccgctcc tccgacgatg gtttcttttt catctaagac cacataccat tgaggaatac
    71281 tttccttatt agcgattcca gtacgaatac tttcctcata cacaacagca ggaactcccc
    71341 attttgatga aaaccacttc gcactttgtt ttactaattc tgggtgttct cttaatttga
    71401 tccagttata tgtaaccaat gtttaaacct ctgactaaaa taaaacgttt acaaaaatta
    71461 tacaagtctt tatgaaaatt agcaatgaca acaatagtga tgtaaaaaaa aacggaaaac
    71521 aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt cttctttctt
    71581 ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc aaattgacag
    71641 cggctagtac accacctgca atactttttg ttgccaatac accgccaata attggtggag
    71701 ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc gcaaagtaag
    71761 caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc gggttcaaca
    71821 cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc gcaatcgaca
    71881 agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata gctaacagta
    71941 aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa ggtttgttca
    72001 cgataaacgg tgcgctatgt ccagctgcga ttgcattaac gtttgcttca atggctggcg
    72061 cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca aagaaccata
    72121 agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca agtcccataa
    72181 atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg aatgcagcaa
    72241 aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt aatgaagcaa
    72301 atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat ttttcatttc
    72361 cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca ataaacaaac
    72421 cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa gaaatcacac
    72481 ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaagc aagaaagcaa
    72541 tcaagacaga aagaatggca aatgtaccgt tccaagcagc agccccgaat cctttccatg
    72601 attcaccacc aaaaattgag ttcatcaatt cttggaaccc gggaatcggt aagttattga
    72661 tcattactgc taatgctcct aagatcatta acggcatact caccataaat gagtcccgaa
    72721 tcgcaactaa atgccgttgt gcaccaattt ttgatgctac aggtataaaa tgtttctcca
    72781 taaatgcaac aaatttatcc ataaatcttc cctcttttct ttactgtaga ttacgcgttc
    72841 aatcgacgat acagatcaat gatctctttt gctaaatctg taaaagctat ggatgtcatc
    72901 agatgatctt gactgtggac tgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct
    72961 tgtgtcagca tctctgtttg agagtgatgt gcttggacta aagattcctc tgcatctttg
    73021 attttttcat ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc ttccattgca
    73081 tcgctttttg catttccgcc aaacatgatc agacccatga tggcttctag atttttttga
    73141 tcttccattt attgtatcct ccgataattt ttattatttg tttattagtt tttctgcttg
    73201 gtcgagtact ttttcaccat tcatcatgcc atagtcagcc atattgatta tatctaaagg
    73261 aatgccttta ggtgctagct tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc
    73321 taaaagtaaa acgtctacat ttttagattc tagttgatta tccgcatctg aagcagatac
    73381 agcaaagatg tctgcttcca ttccttttgc ttctgctgcc ttttgcattt ttgtgactag
    73441 gagacttgta ctcattccag ctgaacatac aagcatgatt gttttctttt ccattttaaa
    73501 cacgttcctt tctatactta cacttgtata taagcaagta gcgtgccaac tttaaaagcg
    73561 ctttaaaaag ttgatatcat cacatttaag gtctatacca agttgcatac acactaaaaa
    73621 agaaagtgca cactaaacac acaaattaat ttttattacc ttttttcttt taacgtgcac
    73681 aaaaaaagtt gtgacacaaa acttgtcaca actttttttc gaataaacgg tattcaaaag
    73741 cttgcatctg cggtaataag tccaaccaag ttaaaaacat aagatgttgt ttttactttg
    73801 ctattcttat tacttgatgc aggacagctt tttcacaacc tctcaataaa cggtgttcaa
    73861 tcagctgacc ctggatttta gctcaaaacg ctggattcac aacctctttt atttcaaata
    73921 accagtaaat acaatgaaca tctacaaaaa acttctattt atttatagat ttcgtcaggc
    73981 aaatccaatt ctctcttatc aatcaaagtc tgataccacg aagcagattt tttcaatgtt
    74041 ctgcttcgtt catcgttcaa ttcgattcga actaatccat atcgattctt aaatgcattc
    74101 attggtgaca cacagtcagt gaatgcccat aacatatagc cttcgcaatt tgcgccttct
    74161 tctgttgctt tgatcaaata acgcaaatgc tgtccgataa attcgatacg gtaatcatct
    74221 tgcacgattc ctttctcatc catataccgt tcttcatgtt ctcttcccat accgttttct
    74281 gttatcagcc aagggatatt atgataattt tcttttagat acatggctat atcatacatg
    74341 attttcggat agatttccca tcccctggaa ttattcattt tcttcccagg taaatcaaag
    74401 acttcataat atcgttctgg atggaaaggt gtctcttcat tccattgata acgaggagcg
    74461 cgaacgcgtt taggataata ttgattgatt ccaaggaaat ctactgtatg atgctgaatc
    74521 gtttctaaat cctctgttgc aggatcgaaa taaatcgatt gtttttgaca caactccagg
    74581 aactcttctg tgtagtaccc ttttaccatc ggatcaaaaa atactcgatt ataaaacaaa
    74641 tcatacatat aagcagcttt ttggtcttct ggagaattag atcgagcata gaccatttca
    74701 ggatttaaaa tacagccaat ccttccacca taatttccgc tgcggtaaat ctctactgct
    74761 ttagccgttg ctaaaacttt atgataattc cacatcatcc acttttttgt gttttgttca
    74821 tgcggccagc gaacagcatc caaatagcaa cgcgtttggg gaacgattgg ttcgttgaat
    74881 gtaaaccagt tttttaccct atcaccgtaa cgatcaaagg caatttgagc atacttagca
    74941 taaaaatcaa ctacttttct tgaagaccag ccatcatatt tttccaaaag aaaagctggg
    75001 acttcataat gttccaaaca aatcatcggt tctacgtctg cttcgatcaa agagtcgatc
    75061 atttcactta tatgctgagc atattcttca tctactatac agttctcata atcagtaaaa
    75121 aaacgagccc aattgataga agtccgataa tgtgtcagat ggatggattt catcagatcc
    75181 acatcctgct ggtattgttc cataaaatta gttgctacgg ctgggccata cccattatgc
    75241 caaacaaaat gttcattttt ataccagcta tctataaacg aatcctgtcc ttcttttttt
    75301 cctttccaac cttccgtttg ccaagcagaa gatgcagcac caagaataaa attttttgga
    75361 atagtaattt tcatgatttc ctcctgtttt tagatcccat cattttccac acgatttgct
    75421 acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag ttgcgtaata
    75481 actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg cattggccaa
    75541 gggaccatta caactgcttt cgccgcaaac cctaaagcgg ttgcaaaata accaatcgtt
    75601 cctgtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag catgatcggc
    75661 ataccaaaaa taactggttc attaatgttg aagatcccag gagcaaggga taacttggtg
    75721 atttgtttca tatcctcccg ttttccaact aacaatactg caatcagtaa accgatcgtg
    75781 actccagacc cacccatact catatacata tcccaaaatg gcatagtaat aatgttcggt
    75841 acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa aagcaacggt
    75901 tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa gacttgcgca
    75961 ataaacatca atagcaaaat acccggtaac ccttgaacga caccttctaa tgggcgttgt
    76021 acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat gataaatccc
    76081 gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa actagcagaa
    76141 acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt cctcaaccag
    76201 caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc tttcgtgtct
    76261 gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt cactaattca
    76321 taaacagttg gattaacaga taaataagac atcactgcga tgataccagg gaatacacca
    76381 tccactttat tcagcttggc taattcgatc cctattagaa aaacagcata gatggtcaag
    76441 aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc taaaaaacgc
    76501 aagtaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga aaacaatgtg
    76561 ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt tatcaccatg
    76621 atatacctta aattattgat tctcgttgcc acgttcccta aaactttggc taatttatcc
    76681 ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca tctctttttg
    76741 tctattagtc agcgcttaca ttttggtgaa cagaaactat ttactcctct tttttaccaa
    76801 atataaaaca aaaaatcatc tgatgtattt atcataaaat acatcagatg aactgtcaat
    76861 tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc atatggacag
    76921 tcattgcgtc aaacgcagca agtacgttcc gcctttcgat ggcctgaacg atctctgtat
    76981 gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta aacaaagcaa
    77041 tcgattcatt aatgattggg ataatatgca gcaaggctat gtttccgcta gcttctgcaa
    77101 tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct cgaaaagaag
    77161 tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt gcatattccg
    77221 ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag tctcgtatca
    77281 tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc cctcgcttat
    77341 cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa gctagagccc
    77401 gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat tcatattcat
    77461 tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg atcttgtcag
    77521 tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt tcatctgatg
    77581 tattttttcg taattattat aacacgtttg aatcatctga tgattttttg tataaaaaat
    77641 cgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa aagactagga
    77701 taaaattcac tcctagcctt cgatcatttt tattttgatc gacaaaaaat atgatgttca
    77761 ataacagacg atcttcattc gattcttgat gtcatctttt gtttttttta atatatggat
    77821 aaaaagtttg attacatcgt ttctattcta gaatttggtc gattctcaat tcttcttctg
    77881 gtgagaaatc taaattatcc atcattcgaa cactttcttg taactgactt aaccgacttg
    77941 ctccgatcag tacacttgta acagattttt gtcgtaaatt ccatgcaagg gctatttgcg
    78001 ctaatgattg tcctcgtgat acggcgattg tttgcagtgc tttgattttt tccagagttg
    78061 atccaacttg ttcttctgaa aggaatggta tttccttacg atgtgctcta gaatcttcag
    78121 gaataccatg tagataacga tttgtcagca acccttgagc aagcggactg aacgtgattg
    78181 cccctagccc ttcttcttct aatactggag atagatcgtc ttctagtagt gcacgagaaa
    78241 acatattgta gcgcatctga tgaataatga acggtgcttc ctttcttttt aaaatttcag
    78301 tcattttttt gtatcttctc cattatagtt ggatatacca atatataatg cttttccttg
    78361 tcttacaagt tgcatcaacg cttctgctgt ttcttccaaa ggtgtatcag ggtctgggcg
    78421 atgatgatag aagatgtcaa cataatctag ccccattctt tggaaacttt ggtcacaact
    78481 tgatatgatg gatttacgag agccccattc cccatatggc ccttcccaca tatgataacc
    78541 tgctttgcta gaaataatca gttcatcacg gtaggcttga aaatcttctc taaaaatacg
    78601 cccaaagttt ttctctgcag ctccagcagg gcctccataa ttattcgcaa ggtcgaaatg
    78661 ggtaattccc atgtcgaatg cgcctcttaa aatctctctt tgattatgga taggatcata
    78721 atcaccaaaa ttttgccata atccgagagg agagataaaa tcggtaattt caaacctgaa
    78781 tgaccggatt tacgatattc cattttttca taacgtgcac tatctgcaat atacaatttt
    78841 tctcctcctc atcaaatctt tttaaaacgc tttcttgttt tctattttta gtttactaca
    78901 ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat atttttttgc catttgtttt
    78961 ttactcgttg atagatcaga aattgattac gtgcttgctg gattttttta tagcgtttcg
    79021 gttgtttttt gtaaaattgc tgatgatagt tttctgctgg ccaaaacgta ccggctggtt
    79081 cgatttttgt gacgattggt tggtggaaag ttcctgaact gtctagtttc tgttttgatt
    79141 gttccgctaa ttctttttgt cgttcgtctt gataaaaaat cactggacga tattgcttcc
    79201 ctctatcttg aaattgtcca aatgtatctg tgggatcagt aatttgccaa tagattgtca
    79261 gcaactcgct ataacttatt tctcttgtgt cataaatgat ctccacagct tccacatgac
    79321 ctgtgtaacc accacttact tgatcataat tcggtttttc aacatgtccg cccgtgtatc
    79381 ctgacaaaac agataggatc ccttttttcg attcaaacgg ctcgaccata caccagaaac
    79441 atccccctgc aaatactgca cgttcttgat agtcagcatc ttgcatggca tgcttggaaa
    79501 agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa taaataaaag tccattacat
    79561 caggcgtcag attcattcgg atagctaatg gacgtagtgt tacttctagt tttgataact
    79621 gttctttaac ggatatattt tctcttgtgt cattctttgt ctgaatcaat acttttcgtt
    79681 cccaatctcg tgtattcgga ttcaaaatca gattataaag tgtttgaatg aatatttgtt
    79741 cctcatccat agattctcct cctttccgaa gaagacaagc tagtgacttt agctgtaatt
    79801 tatcataaag tgaggaaata atcgattgaa tcgtatttat tcaaagaaga taagcagctt
    79861 cagatgatat aagatgatct ttatcttaaa aagcatactc tatggtttgg tttaactaaa
    79921 aaacgacctc tgaaaaaagg gatgttctcc aattctcaga agtcgagtaa agatgattct
    79981 tttttatgag cgataataaa cttcatccaa aaacagacct tgtgcaggga cggtttctcc
    80041 tgcatgttgc cgcactttgc tttcaaatat ttcatcgatt gtttctatcg gcatggcacc
    80101 tgtgccgatt tctagtaatg tccccatgat aatccgcacc attttatata agaatccgtc
    80161 tccgacaaaa gtaaaatgaa gcatttctcc ctttcgttcg atagataatt catcaatcgt
    80221 acgcactgtg gatttttttg attttttcaa tgcagaaaaa ccaatgaaat catgtgtacc
    80281 cactaatttt tcgcaagctg cattcagctt gtccatatcc aactcttgag ggaaataaaa
    80341 gctgtggttt cgctcaaatg ctgaaggaat cacgttgttc caaacataat agctgtattt
    80401 tttgcccacc gcattatatc ttgaatggaa gcgctcaggc atttcttcga ttcttttgat
    80461 gacaatatcc cgcggaaggt aacgattcat aaagtcgatc atttctgcca gtccaatttc
    80521 tgtgtttgtt ttaaaatttg ctacttgtcc tcgcgcatgg gtgccagcat ctgttcttcc
    80581 tgatccaata atttctatct cttcatttgt catctgatgc aatacttttt cgatttttcc
    80641 ttgaatggtt ttatcgtcat ctccaagtct ttgccatcct gaatatcttt ttccgtcata
    80701 ttcgattgtc aacttgatgt ttctcatatt ttagtggctc ctctgtcttc catttggtct
    80761 tctctctacc tttttaagat tgtagcacaa aatatagctc cttgcttctc taagacagaa
    80821 gctatacaag aaaactgtgg aaatatatat ttcagaaaaa caaaaaacag ccaccggatg
    80881 aagtatccgc tgactgttca atttgacggc tcaagtttta ctgtttgaga tgcaagatac
    80941 tcaaccaggc aatgggcaca taattaaaga aaaaactgat tctttggcaa aaacctattt
    81001 tattcagtaa tggccaagaa tttatagaag gaattcttgc cagtccataa atcgccgcaa
    81061 aaagcaaact gatcacagac agtccaaggt ataagtggct tacgtccatt tgcccatttt
    81121 ttcgatagat caagaaaaga agaactggaa acaataaaaa accactgtaa cctaaacctg
    81181 atccgatatt atggacccat gtcgagacat tccacgaaga ttcatgggta ttgatactga
    81241 acaaacctgt aaagatacaa tcacctatac catagaacgc gatggctgct gtaactaggg
    81301 tagctaacac acgagatgta cttacaaatg cagcatacaa tgcaggcaat gacaggatga
    81361 aaaagatccc tgaaaccaca gaccaaataa gaaacgcttt tcttacagga ctggaaacct
    81421 ctccaaatac actgatcacg ctcttcattt gatctaattc aggataatac aatcctaata
    81481 cgtacggagt caaaaaatca ctcagcacgc ctaataataa aaagaaaaaa ccaaatttac
    81541 gtaaaaaatc catgatacgc ctccaataac aattacctat gcttttaatt gttattataa
    81601 acgactttat cttgatttaa cagaaacctc tatttacacg aacatctata taactcatta
    81661 aaaactcgaa tcattttaaa aatactaaat atctaacgtt caacgttcac ttaatccatc
    81721 taaacaatct ttcaatttga ttttttcccc acttctttta cgcgcgccta gctctcctat
    81781 tgcagtaaac aacagatcac ttgacgaatt taaggcagtt tccattgaat cctggataac
    81841 accgataata aatccaatac caacgatttg catcgcgaca tcattagaga taccaaacaa
    81901 actacatgct aatggaatca ataacagtga accgcccgca acacctgaag ctccacaagc
    81961 agctaaagca gacaaaatac ataaaaggac tttcaaaaca aatggtgctt caatccctaa
    82021 agaatgtaca gcagtcaacg tcatcaagga aatggtaatc gcggcaccgc ccatattgat
    82081 cgtagcacct aacgggatcg atatggaata agactctttt gtcaatttca atctttcagc
    82141 caacatcatg tttatcggga tatttgcggc agaacttcgg gtaaagaaag cgggaagtgc
    82201 gctttctttc aaacaaaaga aaatcaatgg atagggattt tctttaataa acaaaaacgt
    82261 gaggaatggg tacacaacta atgcgacaaa cagcatcgta ccgatcaaaa caagtaaaag
    82321 ctgcagatac tctgccaatc cagcgattcc agtcgtagca attgattgat aaaccaatcc
    82381 taaaatccct atcggagcaa atgcaataat cacttgtacg acttgtgaca acgctataga
    82441 tatctgatcc accaactctt ttgtcgattc tgaacgtgct cgaaaagcta gtcccaacaa
    82501 tacagcccag aataaaacgc caaggtaatt cgcatcaata agagattgaa caggattttg
    82561 tgtgacattc gtcaacatag tagataaaac actttccaaa ctttgcggtg ctttttcaga
    82621 tactgtttct tgtaaagtaa taggaacagt aaaaattgaa cttacaatga ctgctacaag
    82681 tgcagaaagc aatgttccca ctaaatataa gatcaaaata ggcttcacga atgtcttatt
    82741 cccaatttta tgcttggcaa ttgacgccat cgtcaagaaa aaaaccagaa taggtgcgat
    82801 tgccttcaag caactgacaa acaacgtccc caatattgaa ataaatgtcc acgtggggaa
    82861 aaagatacca aacaatattc cgactaaaat accaacaaca atacgttgaa ctaacgacat
    82921 atccaaaaac ttctctaaca aatgaccacc ctttcacaat tttaacaatc attgtatcat
    82981 tttatctaaa gaaactaaag gtgcaatttt ctaaaaattc taataataca attggcaatt
    83041 catgctcttg caacattcaa ttttagtatt ggagggttta ttcaatctct aaagaagtcg
    83101 agagaaaact atttatcgtt attattatag ttatccaagt aaaacgtaac taagatgaag
    83161 gacgaaacta gaaatttcat ccttcatctt aattcttaga catagcaatc aattaatatt
    83221 ttccactgag tctcttcttt ctctcactct cactggttta cttacccata aacgatttga
    83281 ttaaagccat ttttcaattc attattcgct gtctcgttaa agtaaagata attatcggga
    83341 ttcccctgat tttgttgtgt caccaatact tttggctgat tttgctgaaa cccgaataga
    83401 tagacgttct ttttcagata tggttctgtc tctgcatcgg aatagacggc tgtgatttga
    83461 tagtctgctt gacctgtccc actttctgac cattctgcag aaacaggcac tccaccaata
    83521 gccatcttcc actccccgtt caaaataact tgagggactt taagaccata taagtccaca
    83581 ctcatttgat tcgtatagct tttgtattgt tgtcccattg tttggcccca tgtagccata
    83641 aatgcctcta attgtgcgga tttgtctgca ttccacagtg tttgttcgac ttgttcgctg
    83701 ctttctgaag atgaagcctc tacagaagaa cttgaagtag atgtttgagt tgtttcagtc
    83761 gttttggaag actctgacga agtagaggta tctgctgttt ccgtacttgc tgcagttggc
    83821 ttgctctctg tgctagttgt tgtagtagtt gcaattgttg ctttcgctgt ttcttttgtt
    83881 gattcttttt tcgattcatt cccgttggta caaccagcta acaaaaaagc tagaccaatt
    83941 gttactaaaa tccttttttt aaattaaaaa actcctttcg attactccca cttctttatt
    84001 ctatcaaata agcaatgtga ttttattgat tttttgtatt ttctcatcca tcttttaaac
    84061 cttttaaaaa ctaagaagca cccgctgatg ttgtctgtaa tacatcagcg ggtgcttctt
    84121 ttattttaat cttcgtgatt ttccaattta cctcacgctt tatatttgta tttgaagaaa
    84181 catagacttt tagtcatcat cgtcttcctc atccaaatct tgtcggacag tttttccttg
    84241 gagtctttgt acttggtaga tgatttcttt attgaaactt aaaagttcaa caagcaattg
    84301 tttcaagcca tatttttctt ctttttcagc taatttgatt gcccgtgtga taaagacatt
    84361 ttggaaatca aaatcttttg caaagttttc caacattgtt gaggctggta aatatttgtt
    84421 ttgaccatgt tcgtaaattt ttccataacg catgacttct tctaaagtag acggaacgat
    84481 ttcattttca atcactaact catgagagat tttattgaac catctcgctt cttgatcagc
    84541 atattctgaa aaaagggtac ttaattcttg cgaagatgta cccaaaacat acaattgtgc
    84601 ttggatcaat ttcatttctt ctaatttaaa atttgccaat acatgcccca ccatggcgcc
    84661 agcagttggt ttatgatgat cgatatctgc ttgttttgtt tcttgagcaa atttttcttc
    84721 cactatattc actgatttac gcctctttta cttttgttga ttggacttca taacctaatt
    84781 tttccacaac tgcagctaat tgttcagggt ggacttcatc tggatcaaag tttgctttga
    84841 ttttgctcgc attaaacaaa actttcgtat tgcttacccc aggctgttga gaaaccgcat
    84901 gttctatttt ttgcatacag cttggacaag ctagtgtatc taattgcata atcatttttt
    84961 tcatcgtagt tcttccttcc tttttcttga tagtcttatt ctagattaca gcttccataa
    85021 aaaaattgat tcttatcaag ttttacgatt tttcttcttt gctttcgtgt ccaaaataca
    85081 ataaacgcat cgcgttaaag ataacgatca agatactagc ttcgtgaacg aacatcccac
    85141 tagccatgta aataaacccg gcaaataagc cgatgagtaa aaataagacg acaccaatgg
    85201 caatcactac attttcacgg gtattcaaaa cagttttctt agccaaacgg taagcatgaa
    85261 ccaacgatcc aaaactagat tgcatcaaga caacgtcact cgtttcgata gcgacatccg
    85321 ttcctgatcc catagcaatc ccgatatcag ccgcagccaa agagggagaa tcattgattc
    85381 catctccaat gaaagcaacg tgcaatcctt gttcttggta ttttttcaca aattgaacct
    85441 tttcatctgg caataattca gcatggactt catcaatgcc taacatatca gctacataat
    85501 cagcagtcat ctgattatca cctgtcagca tcactaaatg ttttgctcct gcttgtttca
    85561 acatagctaa ttgttcagcg acttctggtc gaatcacatc tgacacacca atgatttgag
    85621 tgattttttt gtcgattgct acaataatga ctgagctgcc tctttgttgt aattgatgta
    85681 aatcttgtac ttgctttggt gtgagagaaa gtttgtgagc ggcaatcaat ttggtgttgc
    85741 ccgcacaaac tgtatgacca tcaatctcag ctgtgattcc ttgtcccttg atcgtctgat
    85801 tatctttgac tgctaattgt tgaaaatcaa tgtctttctc ctgagcataa tggatcacgg
    85861 cgcgccccag aggatgatcg gataacgttt ctaatcgagc cactaaagct aagctgtccc
    85921 cttcatcctc tgtatagttc ttgatagtgg atacagcagt agttcctttg gtcaatgtac
    85981 cggttttatc aaagacaaaa gtatcgattt tggaaaaagt atccattgct tcaccgcctt
    86041 tgactaagat gccccgtttc gctccattac cgattcctgc tacattcgaa acaggcgcac
    86101 caatgactaa agcgccagga caaccaagaa ccaaaacagt gatggctaaa cgaaaatcac
    86161 gactgatcag accaacaagc aacgcaataa gtaaaacggt tggagtataa tactgagaaa
    86221 attgatcaat gaatttttcc gcatgtgatt tggtatcttg tgcttcttcg actaattcta
    86281 taattttggc aaaggtcgta tcatctccga ccttagttgc ttcgatcgtc acgtagccat
    86341 ctgctaaaat cgttccagaa aatacgttat ctccactgat tttgacgact tgtcgtgatt
    86401 cacccgtcac tgcggcttca tccgcatacc ctttcccgtc aatgactcgt ccgtccacag
    86461 gaatagctcc accagttttt actaagacga tatcgccttc ttccacgtca tcgatttcca
    86521 cttcttcggt cgttccatct tcttggacaa ctctggcagt cgatggggcc atctcggtca
    86581 aagccttgat tgattcccgc gttttggtca atgttttttg ttccagataa ctaccaaaca
    86641 aaaacaagaa tgtaacgata gctgactcct tgtattctcc gatcataaag gcaccaagca
    86701 cagcaatagt aacgagtaaa tcaattgaga taactttgac ttttactgcc tgatacgcat
    86761 ggattataat aggtatgacc gcaataatag aagctatggc tagagcaatg ttatagaaca
    86821 agacaaatcc agtaaatttc ccgataaaac caaaaataat caacacagct gataaggcgg
    86881 tgatatgatt tcgatgctct tggatccact tttgtaattt catctttctc cctcttttcc
    86941 ttttcattct tacgagacta agtatagata cttatccatc ataaaaaatt gaccactatc
    87001 aagtttattc aaaaaggcgg ttttcttttg gtatactgaa ttcctacagt cattaaacga
    87061 tcatatagaa aaaggaagcg ccgaaacgct tccttaggta gttctatttt tttattcaaa
    87121 gataagatgc tttgtcatct tacgactgaa gaacaacaat caaaaaaggg cagcagccac
    87181 tcattcacaa gaaatatcgt tttttgatat gggaattgaa gcagtggaca aatatctcta
    87241 cttattagct gggaataaga tccaaaaaag cttaatggat tttatccaag aattggaatg
    87301 tactttccac aaaaaattca ctcattccat tcttttaaaa cttttgattc atacagcgtg
    87361 tctaattgag cgtacattga taaacggtca tgaacttaaa attatatcag aagatgacac
    87421 aagaccttct catgaaacaa tttttcatgt aaaaaaggcc ttcaaaaata ttgaaacaga
    87481 atttgggatc actgtttctt atgatgaatg tttctttatc tatgatatta tcgcatctaa
    87541 ataatctttt tttatgtaaa cgagtatttt tttaacttat tgtttaagag tgatacgcga
    87601 attgtaaaat taagttagaa aaataaaaag gcatttatgg tacactcaaa ttgtatttcc
    87661 gacgaaagaa aacaaaggag tgt
//
LOCUS       DAFCNS010000003        61885 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFCNS010000003 DAFCNS010000000
VERSION     DAFCNS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.003"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            74..592
                     /locus_tag="LNE23_000186"
     CDS             74..592
                     /locus_tag="LNE23_000186"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK6930069.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            720..1403
                     /locus_tag="LNE23_000187"
     CDS             720..1403
                     /locus_tag="LNE23_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBK6930070.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1712..1785
                     /locus_tag="LNE23_000188"
     tRNA            1712..1785
                     /locus_tag="LNE23_000188"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1746..1748,aa:Arg,seq:tct)
     gene            1944..2303
                     /locus_tag="LNE23_000189"
     CDS             1944..2303
                     /locus_tag="LNE23_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBK6930071.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2426..4057
                     /locus_tag="LNE23_000190"
     CDS             2426..4057
                     /locus_tag="LNE23_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBK6930072.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4119..4760)
                     /locus_tag="LNE23_000191"
     CDS             complement(4119..4760)
                     /locus_tag="LNE23_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK6930073.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4946..5022
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5042..5350
                     /gene="rplU"
                     /locus_tag="LNE23_000192"
     CDS             5042..5350
                     /gene="rplU"
                     /locus_tag="LNE23_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBK6930074.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5377..5721
                     /locus_tag="LNE23_000193"
     CDS             5377..5721
                     /locus_tag="LNE23_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBK6930075.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5734..6027
                     /gene="rpmA"
                     /locus_tag="LNE23_000194"
     CDS             5734..6027
                     /gene="rpmA"
                     /locus_tag="LNE23_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBK6930076.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6461..9268
                     /locus_tag="LNE23_000195"
     CDS             6461..9268
                     /locus_tag="LNE23_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6930077.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9459..9950
                     /locus_tag="LNE23_000196"
     CDS             9459..9950
                     /locus_tag="LNE23_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6930078.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10053..11042
                     /gene="manX"
                     /locus_tag="LNE23_000197"
     CDS             10053..11042
                     /gene="manX"
                     /locus_tag="LNE23_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBK6930079.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11075..11878
                     /locus_tag="LNE23_000198"
     CDS             11075..11878
                     /locus_tag="LNE23_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBK6930080.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11899..12813
                     /locus_tag="LNE23_000199"
     CDS             11899..12813
                     /locus_tag="LNE23_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBK6930081.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12896..13270
                     /locus_tag="LNE23_000200"
     CDS             12896..13270
                     /locus_tag="LNE23_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBK6930082.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13409..13654
                     /locus_tag="LNE23_000201"
     CDS             13409..13654
                     /locus_tag="LNE23_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6930083.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13826..14890
                     /locus_tag="LNE23_000202"
     CDS             13826..14890
                     /locus_tag="LNE23_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK6930084.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15136..15561
                     /locus_tag="LNE23_000203"
     CDS             15136..15561
                     /locus_tag="LNE23_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6930085.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15554..16003
                     /gene="nusB"
                     /locus_tag="LNE23_000204"
     CDS             15554..16003
                     /gene="nusB"
                     /locus_tag="LNE23_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBK6930086.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16100..16954
                     /locus_tag="LNE23_000205"
     CDS             16100..16954
                     /locus_tag="LNE23_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBK6930087.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16955..18292
                     /locus_tag="LNE23_000206"
     CDS             16955..18292
                     /locus_tag="LNE23_000206"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBK6930088.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18297..18521
                     /locus_tag="LNE23_000207"
     CDS             18297..18521
                     /locus_tag="LNE23_000207"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBK6930089.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18523..19413
                     /locus_tag="LNE23_000208"
     CDS             18523..19413
                     /locus_tag="LNE23_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK6930090.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19422..20240
                     /locus_tag="LNE23_000209"
     CDS             19422..20240
                     /locus_tag="LNE23_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBK6930091.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20347..20796
                     /locus_tag="LNE23_000210"
     CDS             20347..20796
                     /locus_tag="LNE23_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK6930092.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20814..22496
                     /gene="recN"
                     /locus_tag="LNE23_000211"
     CDS             20814..22496
                     /gene="recN"
                     /locus_tag="LNE23_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBK6930093.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22551..22667)
                     /locus_tag="LNE23_000212"
     CDS             complement(22551..22667)
                     /locus_tag="LNE23_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBK6930094.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22740..23696)
                     /locus_tag="LNE23_000213"
     CDS             complement(22740..23696)
                     /locus_tag="LNE23_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK6930095.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23867..24235
                     /locus_tag="LNE23_000214"
     CDS             23867..24235
                     /locus_tag="LNE23_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBK6930096.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24405..24836
                     /gene="mraZ"
                     /locus_tag="LNE23_000215"
     CDS             24405..24836
                     /gene="mraZ"
                     /locus_tag="LNE23_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBK6930097.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24857..25816
                     /gene="rsmH"
                     /locus_tag="LNE23_000216"
     CDS             24857..25816
                     /gene="rsmH"
                     /locus_tag="LNE23_000216"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBK6930098.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25821..26222
                     /gene="ftsL"
                     /locus_tag="LNE23_000217"
     CDS             25821..26222
                     /gene="ftsL"
                     /locus_tag="LNE23_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBK6930099.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26223..28415
                     /locus_tag="LNE23_000218"
     CDS             26223..28415
                     /locus_tag="LNE23_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK6930100.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVTAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28441..29403
                     /locus_tag="LNE23_000219"
     CDS             28441..29403
                     /locus_tag="LNE23_000219"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBK6930101.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29417..30778
                     /locus_tag="LNE23_000220"
     CDS             29417..30778
                     /locus_tag="LNE23_000220"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBK6930102.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30804..31892
                     /gene="murG"
                     /locus_tag="LNE23_000221"
     CDS             30804..31892
                     /gene="murG"
                     /locus_tag="LNE23_000221"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBK6930103.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31907..33127
                     /locus_tag="LNE23_000222"
     CDS             31907..33127
                     /locus_tag="LNE23_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBK6930104.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33283..34608
                     /gene="ftsA"
                     /locus_tag="LNE23_000223"
     CDS             33283..34608
                     /gene="ftsA"
                     /locus_tag="LNE23_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBK6930105.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34631..35872
                     /gene="ftsZ"
                     /locus_tag="LNE23_000224"
     CDS             34631..35872
                     /gene="ftsZ"
                     /locus_tag="LNE23_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBK6930106.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35889..36566
                     /locus_tag="LNE23_000225"
     CDS             35889..36566
                     /locus_tag="LNE23_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBK6930107.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36580..37179
                     /locus_tag="LNE23_000226"
     CDS             36580..37179
                     /locus_tag="LNE23_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBK6930108.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37194..37481
                     /locus_tag="LNE23_000227"
     CDS             37194..37481
                     /locus_tag="LNE23_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBK6930109.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37530..38312
                     /locus_tag="LNE23_000228"
     CDS             37530..38312
                     /locus_tag="LNE23_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBK6930110.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38333..39040
                     /locus_tag="LNE23_000229"
     CDS             38333..39040
                     /locus_tag="LNE23_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBK6930111.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39302..42100
                     /gene="ileS"
                     /locus_tag="LNE23_000230"
     CDS             39302..42100
                     /gene="ileS"
                     /locus_tag="LNE23_000230"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBK6930112.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42149..43675)
                     /locus_tag="LNE23_000231"
     CDS             complement(42149..43675)
                     /locus_tag="LNE23_000231"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBK6930113.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43690..44337)
                     /locus_tag="LNE23_000232"
     CDS             complement(43690..44337)
                     /locus_tag="LNE23_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBK6930114.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44521..44850)
                     /locus_tag="LNE23_000233"
     CDS             complement(44521..44850)
                     /locus_tag="LNE23_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBK6930115.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45027..45755
                     /gene="sfsA"
                     /locus_tag="LNE23_000234"
     CDS             45027..45755
                     /gene="sfsA"
                     /locus_tag="LNE23_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBK6930116.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45771..46784
                     /locus_tag="LNE23_000235"
     CDS             45771..46784
                     /locus_tag="LNE23_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6930117.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46784..48061
                     /locus_tag="LNE23_000236"
     CDS             46784..48061
                     /locus_tag="LNE23_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK6930118.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48124..50826
                     /locus_tag="LNE23_000237"
     CDS             48124..50826
                     /locus_tag="LNE23_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6930119.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50978..51295
                     /locus_tag="LNE23_000238"
     CDS             50978..51295
                     /locus_tag="LNE23_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6930120.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51325..51645
                     /locus_tag="LNE23_000239"
     CDS             51325..51645
                     /locus_tag="LNE23_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK6930121.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51753..53213
                     /locus_tag="LNE23_000240"
     CDS             51753..53213
                     /locus_tag="LNE23_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6930122.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53281..53502
                     /locus_tag="LNE23_000241"
     CDS             53281..53502
                     /locus_tag="LNE23_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930123.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53533..53715
                     /locus_tag="LNE23_000242"
     CDS             53533..53715
                     /locus_tag="LNE23_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBK6930124.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53715..54128
                     /locus_tag="LNE23_000243"
     CDS             53715..54128
                     /locus_tag="LNE23_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBK6930125.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54251..55432
                     /locus_tag="LNE23_000244"
     CDS             54251..55432
                     /locus_tag="LNE23_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6930126.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55503..55667)
                     /locus_tag="LNE23_000245"
     CDS             complement(55503..55667)
                     /locus_tag="LNE23_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930127.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55821..55894
                     /locus_tag="LNE23_000246"
     tRNA            55821..55894
                     /locus_tag="LNE23_000246"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55855..55857,aa:Arg,seq:cct)
     gene            complement(55963..57102)
                     /locus_tag="LNE23_000247"
     CDS             complement(55963..57102)
                     /locus_tag="LNE23_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6930128.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGLEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57401..58036)
                     /locus_tag="LNE23_000248"
     CDS             complement(57401..58036)
                     /locus_tag="LNE23_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930129.1"
                     /translation="MDNRNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58149..58784)
                     /locus_tag="LNE23_000249"
     CDS             complement(58149..58784)
                     /locus_tag="LNE23_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBK6930130.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQIEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58818..59279)
                     /locus_tag="LNE23_000250"
     CDS             complement(58818..59279)
                     /locus_tag="LNE23_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBK6930131.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59409..59840)
                     /locus_tag="LNE23_000251"
     CDS             complement(59409..59840)
                     /locus_tag="LNE23_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK6930132.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59858..60178)
                     /locus_tag="LNE23_000252"
     CDS             complement(59858..60178)
                     /locus_tag="LNE23_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6930133.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60477..61253
                     /locus_tag="LNE23_000253"
     CDS             60477..61253
                     /locus_tag="LNE23_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBK6930134.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61268..61471
                     /locus_tag="LNE23_000254"
     CDS             61268..61471
                     /locus_tag="LNE23_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930135.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61487..61825
                     /locus_tag="LNE23_000255"
     CDS             61487..61825
                     /locus_tag="LNE23_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBK6930136.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT        20400 a        10419 c        12990 g        18076 t
ORIGIN      
        1 agctataatt tgaaaaaaat ttatcgttat tatatgatta agaaggattg aataaagaaa
       61 gaagttgaaa acgatacctg attacaaaaa aataactttt ataagttcta taacgttgat
      121 tatattttta attcttattc ttctcgtcgt cttggttccc cgaatttttg gtttgactcc
      181 ttatattatt gaggacaaag aaatgagtcc atattatgca caaggaaatc tgatatatgt
      241 acgaccgaaa aatacaaatg agattttagt cggagatatt attacatatt atgaaaattc
      301 aggaaaaaaa atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg aagattttta
      361 tacaaaagct gatacaaaga cttatattga gcctggagta gtaaaaaaga gaaatattat
      421 cggtagtccc atctttcaga ttccatatat tggactagtc ctttcaaaaa cggcatttgc
      481 atggattcag gggttattct ttacgattgc tttttgccta acaggaatca cagcatggaa
      541 tacctttgta gagttgaaaa agaagcggag acgaacacag aaatttttgt aagaatgtta
      601 aaaacataaa tgtattccag agatccaacg actcttctga acatgaaaca cgagaaaaca
      661 ctcatgcagc ttgtagattt agtcagttgt tcgtataatg tgtgaagagg aggagtagta
      721 tgcgcaatga aatgatgcat cgtcctgtag tcaaagaaga actattggat tttatgcgta
      781 cccagcaaaa aaaattaccg ggtgagctag gcaaactgga agaagaagct catgtagcag
      841 aagtaccagt tattccccat gaaacagttg tatttttgaa atttttactt ggacagcttc
      901 agcctgaacg aatcttggag atcggagcag ctatcggttt ttcatcaagt gtcatggcca
      961 cgacaatcgg agaaaacgct catgttacga caattgatcg tttcgatgtc atgatcgaga
     1021 aagctaaaaa aaactatgaa cgtctaggac tgacagataa agtgactttg cttgaaggtc
     1081 aagcagcaga tatcttgccg gagttgtctg gtccgtatga ctttattttt atggatagtg
     1141 caaaatcaaa atatattgaa tttttacctg aatgcttacg tttgttaaga aaaggtggcg
     1201 ttttgatggt agatgacatc ttccaaggag gaacgatctt gctcccagat gaagagattc
     1261 cccgtggcaa acgagctatc catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc
     1321 atcccgattt gacatcgaca atcttgccat taggtgatgg tgtgatctta atgacaaagg
     1381 aatcagaaac aatcgagttg tgatacatga ttatggcagc ctgtagataa tcgattgaat
     1441 cgagggctca attcgaactt gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata
     1501 caggaaatct aaataaacag gagtagcatg aagtcaatgt agctcctgtt ttcaattgat
     1561 aaccttagaa cagctgaagg agaatgtctc gttttaaaag attgatttag tagttgggcc
     1621 aaaataattc attatttgct aattaaataa ctatttcgta ttgacagaag caatttaatt
     1681 atctataatt attgatgttc cgctgattct tgtcatagta gctcagctgg atagagcatc
     1741 cgccttctaa gcggacggtc gggggttcga atccctcctg tgacgctaaa aaaccgttag
     1801 aaactgaggt ttctaacggt ttttctttgt tttttgtgcc aatattgttt gttttcaaag
     1861 gcaaataaaa taaaaaaaca tccttatcga gtacaattat aaggagcaaa aaaataaaac
     1921 tcatcagaaa agaggaagaa gatatgacga tactatctag aattttagtg actctagttg
     1981 caattgaatt tttttatatc atgtatatcg aaacggtgag aactgattct gacacgacaa
     2041 gtagggtttt taaaatgagt aaagaagagt taagcagaaa atctgttcaa acattgttta
     2101 agaaccaagg agtgtacaat ggactattag gagtgggact actttatgga gcttatcttt
     2161 cttccgcatc taaagaaatc acaagtatgc tattaatatc tatttttttt gttgcattat
     2221 atggcagttt gactagcgat cgttggattt tactcaaaca aggtgggcta gctctgttgg
     2281 cattgctttc tattttctta taaatataga aaaaataaga atttatctaa caggaaactt
     2341 tgtgaaaaat attactttat ctcatgaaat atgatattct aatgaatggt attttagaaa
     2401 atttcatgaa aaaggagtta gataggtgtt caaaaattat gtttcctcac taaaaaaaga
     2461 attttcaggt tatgatcttt caagatttca gaaagatctt ttggctggga tcacagtagc
     2521 tgctgtagca ttgcctttag ccttggcttt tggggtatca agcggagctg atgcggccgc
     2581 aggtttgatt acggctatca ttgccggtat cgttattggc ggtttatcag gcggttttta
     2641 tcaaatatca ggtccgacag gtgcgatggc agctatcttg atgtctattg cggcaacaaa
     2701 gggaatgcaa ggtgtattgc ttgctacttt tttagcgggt ctatttttac ttgcagcagg
     2761 acttttccga ttaggaacac tgacttcttt tatcccagca cccgtaatta cggggtttac
     2821 ttcaggtatt gctatcatta ttgcattagg tcagatcgat aatttatttg gtgttcactc
     2881 ggaaggtaca aacgttattg aaaaactaag tagttatcaa acgataggat ttccgataaa
     2941 cacagcctct ttgatcatgg gagggctagt cattttaggg atgtttatct atccaaaaaa
     3001 atgggcgaaa cgaatgccta gttcgctgct ggctatcatt ttggcaacag cagttatgct
     3061 attgttccat ttaccagtag caactgtggg taagattcca caaacattga tcagtagtaa
     3121 tcgtttgaat atgggagatt ttagcttttc tgccttgcaa caagtggcag ttccagctat
     3181 tagcattgct ttattaggaa tgattgaaag cttgctatgt ggtgcttcgg ctggccgaat
     3241 ggcaaataag cccttagata gcaatcaaga attaatggcg caaggaattg gaaatctgtt
     3301 gcttcctttc tttggcggga ttcctgctac tgcggccatc gcccgtacaa gcgtggccat
     3361 caaatctggt gcacaaacta gattagctgg tatgatacat gcagttgtct tactgctctc
     3421 catgcttgtt tttgcgccag tgatgtcaca gattccaatg ccagcacttg caggtgtttt
     3481 gattgtgacg gcttggcgta tgaatgaatg ggaaacgatc aaggagctat ttactaataa
     3541 atattggtca gcagttctat tgtttatcct tacgatgatt tgtacagtta tctttgattt
     3601 aagtattgct atcgtgatcg gggtgatcgg cggctgtgta ttttttgttg tcaaaagcgc
     3661 tgctatcacg atttcattag aaacgatcga ttggcaaaga atggggctgc cagaaacgga
     3721 aaagctgaat aactggacag tagtgtatat tagtggtcca ctgtttttta tgtcttcaga
     3781 aaaactcaaa aataaattgg aagaactaaa tcatcacgat ggtgtcattt tctccatgcg
     3841 aggcgtacca agtattgatt tgacggcacg tgcagttttt gatgaatttc aagaaaaagc
     3901 agagcaaaga ggacaaacga ttatctatac ttccttgcag ccagaagtag aaaaacaact
     3961 ttctcacttg tgggaacata cattcacgca tcaacatcaa acagttgctc atgcgttgac
     4021 agctttacat gcgaaatata ctgcaattag ccattaaaac aatgaaaaag aacagacaag
     4081 actgtaaact gaagatttta tggtcttgtc tgttcttttt aatattgctg taaaagtgaa
     4141 gctagtactt gccagtcttg atggataaca ggtacaaggg agcgattgta gaaaatagct
     4201 gctggatgaa aaagtggaag aattcgatag tttttttctg accattcgta ggcatccttt
     4261 tgttctgtta attgtaagac aggccctgta tagagttgac catgattttt ggtaaccgta
     4321 tattctttgc caagtaaacg ttgaagacca atgtttccaa gtgtcgcaat taattttggc
     4381 tggaccttca tcagttcgta atccaatatt ggtgcatgcg caaatacttc ggaacgagta
     4441 ggtgtccgat ttggataaac agtttctgac tgttgtgtcc gtttattgat ccgatgagtc
     4501 acgcgatacg ggcggctcct tacagcactg gtgatataca cctcttctcg tgttaagcca
     4561 gttgaagaaa gtgcctgcat caattctttt ccagcttgac cgctaaaagg aacatgcgta
     4621 tcaatctctg ttttccctgg ggcttctccc acgagcatca gtttaggatg aagaggtcct
     4681 tgcccagcga taaatccttc tacaggacgg ccgttcattc ttaaagaagc ttcttgtatc
     4741 agttcttttg ggtattccat ttatgctcct cctttttctt ttattataag aataaaagaa
     4801 acacaatgcg actattttat ttggtagaac tgtgaggata gaaaagcaga agtattgctt
     4861 gaatttttgt tttacttttt gattttaaat ttagttgact aacggttgct gttctggtaa
     4921 gctagtactg ttgtaattgt ggactccaca gctacaaccg ctcagaacaa gtaataagca
     4981 cagcatatgc tgtcacttcg gatggcgagt ctaagtatat cagaaggagg tgctttatag
     5041 catgtacgca attattaaaa ctggtggtaa acaagtaaaa gttgaagtgg gacaagcaat
     5101 ctatgttgaa aaacttgacg ttgaagcagg agaaaaagtt gtttttgacg aagttatctt
     5161 agtaggcgga gaatctacaa aagtaggcgc tccaactgtt gcaggtgcaa ctgttgaagg
     5221 tactgttgaa aaacacggca aacaaaagaa agtcgttact ttcaaataca aacctaaaaa
     5281 acattcacac cgtaaacaag gtcatcgtca accatataca aaagtggtta tcgacgcgat
     5341 caacgcttaa tagcgtttga gaaagaaggc ctatatatga tcaaaggaac atttaagcga
     5401 aatgacgcag gtcagatcgt ttcattcact ttgaccgggc atgctgatgc tggtccttat
     5461 ggaagtgata tcgtttgtgc gggtgtttct gcgttagcga tcagcactgt caacgggatt
     5521 gcctcattag ccggttttga gccgattgtt gaaatgaatg aagaagaagg cggttatctt
     5581 tataccgaag tcacttcagg aatgacacaa gaacaaaaca atattgctga gattctatta
     5641 gaaaatcttt tattaggatt acaatcgata gaagctgaaa actcagaata tatccaaatt
     5701 aaaacaatta atgataaata ggaggtgcag accatgttgt tatctatgaa tctacaatta
     5761 tttgcccaca aaaaaggtgg cggttctaca tctaacggcc gtgactcaga atcaaaacgt
     5821 ctaggtgcta aacgtgctga tggacaaaca gttacaggtg gatcaattct ttaccgtcaa
     5881 cgcggtacaa aaatctatcc aggtgtaaac gtgggtatcg gtggagacga cactttattt
     5941 gcaaaagttg atggcgtagt acgtttcgaa cgtaaaggcc gtaacaaaaa acaagtgtct
     6001 gtttatccag tagctcaaga agcttaattg aatcttatga ttaaagaggt tgtgacaaga
     6061 cttttgtcac agcctttttt gtttataaaa ataaaagttg tagtggaggc tacgaagttt
     6121 actttgtctt tttacactta atattttttt gccaatttgg aatcaggatg ttttgataac
     6181 tatcgttttc aattgatgga agctatattg tttgctatca gtgaaataat tatatggtaa
     6241 acagtgttct ttttcttgtt tccattataa aaatcgtatc gggatttttc gttcttttaa
     6301 attgaacatt tctatctatc cgcgtgttat caacaaaaca aaatcaggta aatagttgac
     6361 gaaacagaaa taaatacata gtattatgtt ttgaagccta agtacaatag tctatagaaa
     6421 aggagcgata ccattggcaa aacgcattga ccgaatttat atgtatgtca aagaaaaaac
     6481 cgctcattta acaaaggcag aatatgatca aggagtaaca acacaggaaa ttgctgaagt
     6541 gttagggatt cagcgaacaa atagcagtaa agatctgaat cagctagtgc gcgaaggaaa
     6601 actgataaag acagacggac gaccagtacg ttacatatat caaacactcg ttacgcacgt
     6661 aaagccagtt acaaaacatg tagtcagtta caaagaaacg ccggtctctg aagaaaaaaa
     6721 tacactccaa atagatacta aggatatctt tgcaaaaatc attggagcta acggcagcat
     6781 gaaaaattca gtggaacagg caaaagctgc gattttgtat ccgccaagag gattaaattg
     6841 tttgattaca ggacctaccg gatcaggaaa gacttatttt gcacacgcga tgtatcattt
     6901 tgcaaaagcg catcatgtaa tagatgagga aacggaactg attgttttca attgtgctga
     6961 ttatgccaat aaccctgagc ttttgatgag tcatttattt gggtacgcaa aaggtgcttt
     7021 tacaggcgca gatgaagaaa aaatggggat tatcgatcaa gcggatggcg gtatgctgtt
     7081 tttggatgag attcaccggt tgccgcctga aggtcaagaa atgatctttt actttatgga
     7141 tcatggaact tacagtcgtc ttggggaaac aacgaaatcc catgaagcaa atgtacggat
     7201 cgtaggtgcg accacagaag acccaggttc ttctctatta gaaacttttg ttcggcgtat
     7261 cccaatcaat atcaaacttc cggcttttga aaaacgtcct gcaaatgaga aagtcgattt
     7321 agtaaaaatc atgattgccc acgaagcaaa ccgcacgcag cgaaagattt ctttgacaga
     7381 agatgtagca aaagcattga tcggcagtgt gacctatgga aatataggac aattgaagtc
     7441 aaatatccag ttagtatgtg cacgaggatt tctaaatcat atgcaatcac ccgaaatctc
     7501 gatcacaatc gatgatttga cagaagggat tcgtagcgga ttgattcaat tagcaagcaa
     7561 ccgcagtgca atggcagagc tttctaaaat attggagcca aagataactg tagcaccaaa
     7621 tgatacgatc atgaaaatcc aatcggactc ctatgagtta ccttataatc tatatgatat
     7681 catcggagat aaagcagctt tactgaagtc ggatggtttg gatcaagaag caatcaatca
     7741 tttcatttct acagatatca acatccatct gaagtctttc tataaagacc atggtttttc
     7801 ttttaatgca gacagcaaat tagcagaatt tgtagatccg aaaatcatag aagtgaccaa
     7861 tcagatttat gtaatggtca aaagatcttt accatatgag tttcagcaaa actttattta
     7921 tgctatgagc ttgcatatca gttctttttt gaaaaaaatc aacttaggag aagaacggca
     7981 tacgaatgat aacatccgtg agatggcttt tgattttcca gatgaatatg ctgttgcgaa
     8041 agaagtgcgt agttatatcg aaacgtattt tcaagtggag attccagaaa gtgaagacta
     8101 ttacttgacc gttctactcg tctcgttacg ggcaaatcaa gcaagcggca gaatcggtgt
     8161 agtcgttgca gctcacggaa acagcacagc aagcagtatg gtccaagttg tccaacagtt
     8221 gttagatgca gagaacgtac gagcagtaga catgcctttg gatatggatc cgaaaacagc
     8281 attggaccga attgaacgca atgtacaaga agtagatgaa ggaagcgggg tcatactctt
     8341 agtagatatg gggtcccttg cgtcgtttaa ttcgcaaatt caaagagaca ctggaattcc
     8401 agtacgtact gttgatatgg taacaacttc tctagttttg gaaactgtac gaaaggtctc
     8461 agtattaggg acagatctag atatgttgca tgaatcattg aaaaattttc gaggttacgc
     8521 agaaatatct tctgtagaat ctgttcagga agcaaaacct attcataaga aaaaagctgt
     8581 tttggctatc tgcgcatctg gcgaaggtac agcacaaaga atcaaagaat tgatcgaacg
     8641 agcggtaaac aagcgtcaag aaacagaatt agaagttcta gctttgtcga ttgtggaagt
     8701 gaaagaagaa ctgccagtta tccaaaaaga atatcagatc atagctacta ctgggattac
     8761 tgatttgaat atccaagccc catttattcc tttagaacgt tttattgatc aaaatatcga
     8821 agtcattttg gatcagttgc tgatggaatc agaattggag gaaactgaat tcatctccct
     8881 agatgaagag tcagctaaaa atacgtgtgt tgagtttatc tctgataact tcatctttat
     8941 taatggtagt aagttaatcg atccaatgtg gcaattttct acacaaatct ctcagacgac
     9001 gggaatcggc gatgaagaat atggattcaa aatcaaccta gtgatgcaca cagcaggaat
     9061 gatcgaacgt attattcgga atgaaccatt gactgtggaa gaaaacgaat tgacaaatac
     9121 aacgaatgat cctctttata gccaattggc agcctcagtt gttctgttgg aagatcagat
     9181 aaaagtaaaa gtgccgatag aagaaatgta ttatctgcta cgtctagtcc ataatcagtt
     9241 agataaaaaa gaatacactg tgccatgaat acacaaaaac gatacacttt tatagtgtat
     9301 cgtttttgtg tattggttaa gaatccttta aattcaatta gtgtatcgta aaaaagttgg
     9361 cacgcttttt gcttatagta gtgtgtgcta ggaggtggtt gttcacacag aaatgatagg
     9421 ggtttctgtg aagtagtgag gaagaaggag ttttgcagat ggatattcga ttggcacgta
     9481 tagatgatcg tttaatccac gggcaagtcg caacagtttg gtcgaagcaa acaggtgttg
     9541 aacgtatcat tgtgatcagc gatgtagtcg ctcaagataa attgagaaag tttctactaa
     9601 aagaagctgc accgccgggg atcacagcaa atgtaataac cgtgcagaaa atgattcaaa
     9661 tctatcatag tggattatta tcacgagcaa aagtgatgct tttattcacc aatccccaag
     9721 atgttgaagc ggttgtccgc tgcggtgtac agttgaagtc gttgaatatc ggcggtatga
     9781 gttttactga aggcaaatca atgattacca attttgtttc agtaaatcaa caagatattg
     9841 attcctttcg ttacttgaac agtcaagaag tggagttaga gattcgaaaa gtacctgctg
     9901 acaggaaaat ccttttgatg gaactactag cgaaaatcaa aaaggtgtag caagaatggt
     9961 tgttggaaaa ggtcaaataa ttctgttgta atggttagca attattaata tttttgaatt
    10021 tgttacaaca aaagtaaata ggaggtataa atatggtagg gattatccta gcaagtcatg
    10081 gacaatttgc tgaaggaatc ttacaatccg gttcaatgat ctttggcgaa caagaaaacg
    10141 tcaaagccgt gattttgaaa ccaagtgaag gtccagatga cttgagagcc aaattggaag
    10201 aagcggttgc ttcttttgat aatcaagacg aagtgttatt cttagttgat ttatggggcg
    10261 gaacaccgtt caatcaatca aacacattat tcgaagagca caaagataaa tgggcaatcg
    10321 tcagcggttt gaatttacca atgttgattg aagcctacgc atcacgcttt tcaatggaat
    10381 ctgctcatga aattgcagca cacatcattg aaacagcaaa agacggtgta aaagtaaaac
    10441 cggaagaatt agaaccggca gaagcaccga aagcagcagt tgaagacgca caacctaaag
    10501 gagcacttcc agaaggaact gtggtcggag acggaaaaat caaatatgta ttagcacgtg
    10561 ttgactcacg tctgttacat ggacaagttg cgacagcttg gacaaaagca gtgcaaccaa
    10621 accgtattat cgttgtttca gatgcagtat caaaggatga tctgcgtaag agactaatcg
    10681 aacaagctgc tcctccaggt gtaaaagcaa atgttattcc tattagcaaa atgatcgaag
    10741 tagcaaaaga ccctcgtttt ggaaatacca aagcattgct tttatttgaa aatcctgaag
    10801 acgtattgac agctgttgaa ggtggcgtag acatcaaaga attaaatgtt gggtcaatgg
    10861 cgcattcggt cggtaaagtc gttgtaagta aagtattgtc tatgggacaa gaagacgttg
    10921 aagcgtttga gaagcttgaa caaaaaggtg tgaaattcga tgtacgtaaa gtacctaatg
    10981 attcacgcga taacatggat gatattttga aaaaagcaaa agctgaatta gcaaaagcgt
    11041 aataaataat attaaaattt ataggaggct tatcatgtct attatttcaa taattttagt
    11101 cgtattagtt gcctttctag ctggtatgga aggtattctt gatgaatttc aatttcatca
    11161 accactagtg gcatgtacat tgatcggttt agttaccggt aacctcgaag ctggtatcgt
    11221 acttggcggt acgcttcaga tgatcgcttt aggttgggca aatatcggtg ccgctgtagc
    11281 accagatgct gcattagcat cagtagcatc agcaattatt ttagttttag gtggacaagg
    11341 tgttaaaggt gttccttctg cgatcgcgat tgccgttcct ttggctgttg cagggttgtt
    11401 cttaacaatg atcgtacgta caatcgctgt tccaatcgtt catttgatgg atgcggctgc
    11461 tgaagaagga aacatccgta aggttgaaat gtggcacatc attgctgtat gtttacaagg
    11521 ggttcgtatc gcggtcccag cagcagcgtt gctatttatc ccagcacaaa ctgtgcaatc
    11581 attccttgaa tcaatgccag catggttaac agatggtatg gctatcggtg ggggaatggt
    11641 cgtagctgta ggttacgctt tggtaatcaa catgatggct acaaaagaag tatggccatt
    11701 cttcgttatt ggtttcgttg tagctgcaat ttctcaatta acattgattg ctctaggtgc
    11761 tttaggtgtt gcattagctc ttatttactt gaacctttct aaaatgggtg gttcatcaaa
    11821 taatggaggc ggaagcaact ctggtgaccc tttaggcgac attttgaatg actattaatc
    11881 ttgaaggagg aaaataaaat ggcagaagaa aaaatcaaat tatcaaaaaa agatcgttta
    11941 gctgttgcgt ggcgctctac cttcattcaa ggttcatgga actacgaacg tatgcaaaac
    12001 ggtggctggg cattttctat gattccagca atcaaaaaat tatacaaaac aaaagaagaa
    12061 cgttctgcag cattaaaacg tcacttggaa ttctttaata cacacccata cattgcttct
    12121 ccaattttag gggttacatt ggctctggaa gaagaacgtg cgaatggcgc gcctgttgat
    12181 gatgtagcga ttcaaggggt taaagtaggt atgatgggtc ctttagctgg tgttggtgac
    12241 ccagtgttct ggtttacagt acgtccaatg ctaggtgcac taggtgcttc attagctatg
    12301 ggaggaaata tccttggacc aatcatcttc ttcttaggtt ggaacattat tcgttgggca
    12361 ttcatgtggt atacacaaga atttggctac aaagctggtt ctaaaattac tgatgacctt
    12421 tctggcggat tgttacaaga cgtaacaaaa ggtgcttcaa ttttagggat gttcgtatta
    12481 gctgcccttg tacaaagatg ggtatcaatc aaattcttac cagttgtatc aactgtaaaa
    12541 ttagataaag gtgcttacat cgaatgggat aaattgcctt caggtggtga aggaatcaag
    12601 agtgcatttg aacaagttaa taatggctta gccctttctc ctgaaaaggt aacaacatta
    12661 caagacaact tagaccaatt gattcctggt ttagctgcat taggattaac attgttatgt
    12721 atgtggttgc ttaagaaaaa ggtttctcca atcgttatca tccttggttt attcgtagtc
    12781 ggtgttgttg gacacgtcat cggactttta taatccataa aaatatagct cgggcgttgt
    12841 cccgagcttt ttttatatag tatactgttc ataaacaaat atctggaggg agaaaatggt
    12901 tcaatcaatc aatacaaaag tagacttggt aattgatgct acttcgttta cgggtctgac
    12961 agattacggg caaatcatga taggtgataa tggttttgag ttttaccatg cgcgagatag
    13021 acgcaaatat atccaaattc catgggaaga agtggattat gtactagcat cagtcatgct
    13081 aaagggaaaa tggattcctc gatatgccat tcaaacgaaa aaaaatggta catacacatt
    13141 ttcttccaaa gaacctaaaa aagtgcttcg tgcgatcaga gaatatgtag atgaagatag
    13201 aatggtacaa tcattgagtt tctttgatgt agtgaaacgt ggtattcgta gtatgtttaa
    13261 acgcaagtaa aaaataaaga aaacttctaa gaggaggttt tctttatttt tttactttgc
    13321 agaagaaaat atgaaatagc aaaaccaatt atttgtcccc tttaaatccc atatgctaaa
    13381 attgattagc aaagaaggag gttatattat gagttggtta tggacattga ttgttggagc
    13441 cgtgatcgga gcaattgcag gtgcgatcac cagtaaagga aattcgatgg gctgtgtaac
    13501 gaatatcatt gctggactag tcggttcaag tattggacaa gcgattttcg gcgaatgggg
    13561 accaaaagct gcaggtatgg cacttattcc atctatttta ggcgcagtga ttttggtagc
    13621 agttgtctca tttttctttg gaaaacgatc ataagttttt acaaaatagt aaactaaaag
    13681 gagttgcagc gagactgtga ctcctttttg gctttattta tttaaaataa aaagaagttt
    13741 tccggaaatt tttatttaac tgtcgtgtac gaagaaaagt ttgctataat ggaaaaaagc
    13801 gtgcaaagaa agagggagtt tcattatgat gctacgtgta gaaaaattgc gcaaaaaaat
    13861 gcaagaagaa aatttggatt catttttggt taccagtccg tacaatcttc gttacttaac
    13921 gaactttaca ggtacgacag gattagctgt tattactttg gaaaaagcat tttttattac
    13981 tgattttcgt tataccgagc aggcggcagc gcaggctcaa gggtttgaaa ttattaaaaa
    14041 tgtcggacct atttttgatg aggtagcaaa cttagtacgc aaagaagaac tgcactcatt
    14101 agcttttgag gaaacaacag tctcattttt agaatattcg gttttagaag aaatcatcga
    14161 tgcggaatta gtacctgttt cacagatgat cgaagaactc agagaagtga aagatgaaga
    14221 agaaatcgct attatcgaaa aagcatgtca tattgcggat atggcatatg atcacatcct
    14281 gaaaatgatc caaccgggca tgactgagat tgaagtcgca aatcaactag acttttatat
    14341 gcgttctctt ggcgcttcag gtgtttcttt tgatacgatc gttgctagcg gattgcgttc
    14401 ggctatgccg catggtgtag caagcaaaaa aatcatcgaa caaggtgact tgatcacatt
    14461 ggactttggt tgctattacg aagggtacgt ttccgatatg acacgtacat tcgctattgg
    14521 tgatcctggg gaaaaattaa aagaaattta tcaaatagtc ttagatgctc aattagcagt
    14581 tattgatgct gcaaaaccag gaatgactgg tgtccaatta gatgcagttg ctagagatta
    14641 tatttcaaaa catggttacg gggaagcatt tggacattct acaggacatg ggatcggttt
    14701 agagatccac gaaggaccaa acgtttcttc tcgtgcagaa aagcaatttg taccaggaaa
    14761 tgtgatcact gatgaaccag ggatctactt accaggaatc ggtggagtac gtattgagga
    14821 tgacttgttg atcacagaag atggaaatcg tgtgttgact cgttcaccaa aagaattgat
    14881 catcttatag tttcatcaat agaagaagta agcgtaaaaa tcagtgttca cagactttgt
    14941 tatagaaaat cactcgattt aatgatacac tatagaagaa cttttaagct gtaagagata
    15001 ataaagttgg gtgttttctc caattcatag cagtttgcag ctgatcggcg gcagttttct
    15061 taccacgtga tcactatgcc gcatcataga gaattcggat gtatctcgtg cttaggcaaa
    15121 gggaggaata aataaatggc agatgaaaaa aatttgattt taaacgcaaa ccaagattta
    15181 ggagagatcg tgatcgctcc tgaagtcatc gaagtaatta tcgggattgc agcttctaaa
    15241 gtagaaggcg tatacggaat gcgcggaact tttgccagca atgtcactga attattagga
    15301 cgtgcagctc acggaaaagg tgtttactta gcaaacgagg aagatggctt gaaagtagat
    15361 ctttactgtt atctagaata tggtgtttca gttcctaaag tagcaatgga aatgcaggaa
    15421 cgggtcagcc agcaagtcct tttcatgacg gatatcgatt tatctgaagt caatatccat
    15481 gtagtagctg ttgttcctga aaaattagaa caaccaacaa tcgaagaact ttttgaagac
    15541 gaagaggaag agaatgaata aagaattatc aagacacgag attcgggaaa tggcattgca
    15601 ggctttattt ccattagact tcaatgcaga cctaacaaaa gaagatgcga ttttcaacgc
    15661 aatcgaatta gaccaccgcg acatgatcaa cgaagatgaa tcagagtttg ttcctgttta
    15721 tcttgatacg ctagttggcg gtgtctgtgc aaaaaaagat gaattggata aagtgattga
    15781 aaaacattta aaaaacaatt ggcacatcaa tcggatctca aaaatggatt tggtgatctt
    15841 gcgtatcgcg atttttgaaa tgacttatgt gacagatgta ccagcggctg ttgctttaaa
    15901 tgaagcaatc gagttggcaa aaacatttag tgatgaccgt tcacgtaaat tcgtcaacgg
    15961 tgttttatcc aatgtattga aagaattaga agctggggcg taagtcctag cttttttcta
    16021 tgaatcatca taaacctgtt ataaatcggg aaaattctat ggtagaatga aaacacaaaa
    16081 aatatctggg agtggttaga tggcagaatt gattaacgga aaagaattag ctgaaaaaat
    16141 gcaggcagag attgctgaaa aaatccaaaa attgaaagat aatggtatcc atcccggatt
    16201 agtcgtttta ttggtagggg aaaatccagc cagccaaatc tatgtaagaa ataaagaacg
    16261 tgtagcaaaa gaaatcggaa tccattcatt ggtagagcgt tatcctgaga cgatttctga
    16321 agacgaacta cttgcagaaa ttgaaaaata caatcaagat cctagttttc atggtatatt
    16381 ggtccaatta ccattgccaa agcatataga tgaagaaaag atcctgttag ctatccgccc
    16441 tgaaaaagac gtagacggtt ttcatccgat gaatttaggc agactccttg cgggtaaacc
    16501 ggacaagatt ccttgtactc catacggcat catgaaaatg tttgaagctt attctatcga
    16561 tccggcgggt aaacgagcag tagttatcgg acgaagcaat atcgtcggta aaccgatggc
    16621 tcagctactc ttgatggcag atgcaactgt tactgttgct cattctaaaa ccgaaaatct
    16681 ggctgaactg gcacaagaag cagatatttt agtcgtagca ataggacgtg gacattttgt
    16741 cacaaaagaa ttcgtaaaac caggtgctgt agtgatagat gtagggatga atcgtgatgc
    16801 taacggtaag ctgatcggtg atgtgaaatt tgatgaagtg gagcctattg caggctggat
    16861 cactcctgtg ccaaagggtg tagggccaat gactatcacg atgctgatgt atcaaaccgt
    16921 aaaatctgcc gaagcttttg gagcaggtga atagatggca caagaatatt taactgtcac
    16981 tacattaacg aagtatttga aaagaaaatt tgatgccgat ccttatttag aacgcgtata
    17041 tttaacaggc gaaatatcga atttccgttt acgtgccaat gcccatcaat atttcagctt
    17101 aaaagatgac catgcaaaaa tatctgctat tatgttcaaa ggagcattcc aaaaattacg
    17161 attccagcca aaagaaggaa tgaaagtcct tgtcgttggg cgtatttcat tatatgaagc
    17221 gggaggttct tatcaaatct atgtagagca catggagcct gatggaatag gtgctttata
    17281 tcaagaattg gaagaacgaa aagaaaagct gatgaaggaa ggcctttttc aagggcctaa
    17341 aaaaatgctg ccacgtttcc caaagcgaat cgcggtcctt accagtccaa gcggagcggt
    17401 tatccgcgat atcatcacga cggtgaaaag aagatatcca attgtccaat tggttttgtt
    17461 cccaacagtt gtacaaggaa accaagcagc ggacaatgtt gtcaggaata tccagagagt
    17521 agaagagatg ggcaatttcg atacaatgat catcggtcgt gggggtggtt cgattgagga
    17581 tttatggccg tttaatgaag agcgggttgc tcgggcgatt tatcaagcta atacgccggt
    17641 gatttcatct gtaggacatg aaacagatac cacgatcgct gatttagtag cagatgttcg
    17701 ggctgccact ccaacagcag ctgcagaatt agctgttcct gttttgtcag aagagttgat
    17761 gcgcattcaa gaacggcaag ctagattgca gcaggcgttt gtacgccaga tccagcgcaa
    17821 acaagaacgg ttcgagcgtg ctcgacgttc ctatgtattt cggcagcctg aaagattgta
    17881 tgaaggtcag accatcaaat tagaccagtt gaaacagcga ttatatcaag ctacacagca
    17941 aatttatcat gcgaaagaaa aacaaacgat cgcattaagc caccaattgg aacaagtggc
    18001 tccgctttat cgagttaaag cagctcgcca agaaactgct tatctagaaa aacgcttaga
    18061 agagaaaatc caacaatata tgaaacaaca gcagcagcgt tttgctcaag ctgttcaatc
    18121 tcttgatttg ttgagtccat tgaaaatcat gggaagaggc tacagctata cgacgttaaa
    18181 tgatcaagta gtaaaaagtg tcaccgagat tcatcaaggg gatttgctga cgatccatta
    18241 tcaagatgga atcgttaaag gacaagtaca agagatagac aaaggagaat aaaaatatgg
    18301 aaaatccaac ttttgaagag tcactgcaag aattagaaaa aattgtcatg cagttagaac
    18361 aaggagacgt tccattagaa tcagctctag attcgtttaa acgaggaatg gaattaagca
    18421 aacattgcca agatacatta gctaaagcag aagagacctt gacgaaaatg atgacagaat
    18481 caaatgaaga agttgatttt gacggaaacg aggaaagctg agatgagatt aactgacttt
    18541 tcagctattc atctacctgc tgtagaaaaa gaaatcttgt cttttttaga tgagcataca
    18601 acggatagag gactctatga tgcgatgagt tattcagtga aagcaggcgg gaaaagaata
    18661 cgtcccttat tacttttaac ggccgttgct tcctttgatg agcctattga tgttcccgtc
    18721 tatcaagtag cagctgcttt agaaatggtg catacgtatt ccttgatcca cgatgatttg
    18781 cctgcgatgg acaatgatga tcttcgtaga ggaaaaccta ccaatcataa agtattcggc
    18841 gaagcattag ctatattggc cggagatggt ttgcttacag gagcattcca gctaatcagt
    18901 atggcacact taggtaattc tcctaagctt ctgctgcttc agcaattagc tgtttgtgcg
    18961 ggatcacaag gtatggttgc tgggcaagct gcagatatcg agggcgaatc gaaaaagctt
    19021 tctttagaag aattagcatt tattcatgaa agaaagacag ggcatttgat tcgttatgcc
    19081 ttgctggctg gtggcatatt agcgaagcaa cctgaagaaa tcttattgct acttcaacga
    19141 ttagccgaac atttaggttt agcttttcag ataagagatg atctactcga tgtgattggt
    19201 acaacgaaaa cattagggaa aacagctggt aaagatgaac gtatggaaaa aaatacttac
    19261 ccgcgtttat taggcctaga aaagacacgt gaggcgttgg agattgagct gttatccgcc
    19321 aacaagatca tagacaaatt agaagaggac gttttttctt ttgatggcgg gctgatgcgc
    19381 cagatgatca agcaatttga tgtggagaaa tgagggaaag aatggaaaaa gaacgcgtag
    19441 atgtattagc ggtcaaacaa ggattatttg aaacaagaga acaagccaaa cgttcagtga
    19501 tggctggttt gatttacaac gaaaaaaacg agcggtttga taaaccggga gaaaaaattc
    19561 cagtgagtag tgaattaaga gtcaaaggta aaaaacttcc ttatgtttct cgagggggat
    19621 taaaattaga aaaagcactc aaagcatttg atctatctgt tgaaggaaaa acattgttag
    19681 atattggtgc atccactggc ggatttactg atgcagcttt gcaaaatggc gcaaaaatga
    19741 gttatgcttt agacgttggc tacaatcagc tagcttggaa aataagacaa gatcctagag
    19801 tcgtcgtaat ggaaagagtg aattttcgtt atgcaaaacc cgaagacttt accttaggaa
    19861 ttccagaagt tgctgttatc gatgtttctt ttatttcact aaaactgatg ctgcctccat
    19921 tacatgctat tttaaaagaa gagggagaag tgattgcttt aatcaaaccc caattcgaag
    19981 caggcagaga agctgtaggg aaaaacggaa ttgttcgtga ccctcagacc catcaaaaag
    20041 ttttagaaga cattttgtca tttgcggcac aaggtggata tgatgtgctg gaattaagtt
    20101 attctccgat cacgggggga gaaggaaata tcgagttctt ggctcacttg aggaaagtac
    20161 ccgaaagcgg aacaatcaat tcagctatta atatggcaga agtggtttca aatgcacacg
    20221 aacagtttga ccataaataa attgtaactg tgaaaatcaa gtgagaagta tgcggattct
    20281 tctttattat accaaggaaa taagctatca ttgattaaga agaatatgct aaggagcgaa
    20341 gacgtaatga gaaaaaaaga tcggcatcgt ttgattactc gattgttgag tgagcaagat
    20401 atccgaaagc aggaagaatt tgttgaaatc ctgaaaagca aaggaatatc tgttactcaa
    20461 gctacgattt ccagagatat aaaggaactg aaactgatca aggtaccggc ttcagatggc
    20521 ggctaccgtt atagcatacc agctgaaaca aacgaagatg taaatactaa attggaaaaa
    20581 ctattaaaag acgctttcgt ggcagttgac caaatggaaa aatttgttat tttgaaaaca
    20641 ttgccgggaa atgcttcggc agcagcaaat ttgattgata aacgttacaa gaaagaatta
    20701 ttttcgatta tcaatgatga cgataatgtt ttgatgatta cacgaactga agaccaggca
    20761 gttttattga agaaagactt cctgcattac ctttaagttg aggagcgaat gtgatgctac
    20821 aagaaatcag tattactaac tttgcgatta ttccggaatt gcggctttct tttcacgaag
    20881 gaatgaccgc gctgaccgga gagactggtg cgggtaaatc cattattatc gatgctttag
    20941 gtctgttagc aggaggaaga ggttcaagcg attatatacg tcaaggagca gaaaaatgtg
    21001 tgcttgaagg gttgtttgaa ctaccaaaac aagaaggatt ctctgaactg atggttgaat
    21061 tagggatcga aacagatgag gacaatctga ttgttcgacg ggatatgtcc ttaactggaa
    21121 aaaatgtttg tcgagtgaat ggtcatatta tcacattagc taatctgaga agaatcggca
    21181 gttatttagt agatatccaa ggacaaaacg aacatcagga actgctccag cctgaatcac
    21241 atcttgcttt attggatcgt tttggagatg tagctttcca acagaagaaa aagagttatc
    21301 agcaagaata tatctcttat cgtgagctgg aaaaaagggt acgaaaagtg cagcaaaatg
    21361 aaaaatccta tgttcagaga atagatatgc tccatttcca gcaagaagaa atagctgctg
    21421 ctgatttgca agtcggagaa gaagagaaat taaaagaaga aagagaaaaa cttagcaatt
    21481 atcaaaaaat tgctgatgga ttagctgcag gttacggagc actgacgaat aatgaacaaa
    21541 acagtgtaga tggtgttggc ttggccgttt cagaactgca agggatcgcc catctagatg
    21601 tggagtatga agcaatttat gaaaatatcc aaagtgctta ctatttgctg caagatgcaa
    21661 tcggcgatat gagtcgtcag attgatttat tggagttaga tgagagtcgt ttagaagaag
    21721 ttacgcaacg tttagaatta atccgacagt tgaaacgtaa atacggcgaa tccattgagt
    21781 caatattggc ttactatgac gaaatcaccg aagagctagc ttcttctgat ttttcagaag
    21841 gtcaattgga caaaatgaaa gaggaactag agcaaaaaga gcttctactt cagcaacaag
    21901 ccgcagatct tcatgaagcg cgtaaaaaaa tcgcaaaaga attggaaaaa tccattctgc
    21961 atgaattgaa aagtctgtat atggaaaaca ctgagtttga ggtacgtttc ttaaaagaag
    22021 aaaatagaca attgaatcat gatggatttg accaaatcga attttacatt acgaccaatc
    22081 caggagagcc actgaagcct ttggtgaaag tcgcttctgg tggcgaactg tctcggatgt
    22141 tgcttgcttt gaaaactatt ttttcttctg agcaaggtgt gacaagtatt atttttgatg
    22201 aagtggatac aggtgtgagc ggaagagttg cacaggccat cgcagataaa atttctcaaa
    22261 tatcgaaata ctctcaagtt ttgtgcatca ctcatctgcc tcaagttgct gcagtcgccg
    22321 actatcaata ttatattgtg aaagcagtga ttgatggacg tacgcaaact tctgtttcag
    22381 agttgaaaac aaaagaaaga gaagaagaaa ttgcacgtat gctggctgga agtgagatta
    22441 caaaactcac actcgaacat gcaaaagaac tattgaaact cgcaaaaagg acctgagcca
    22501 cctcgcagtg gttcaggtcc ttttcagcag ctgatagcta tattttattt ttacatgaca
    22561 ctcattacag cagtcaatac aacagatcca atcgttaaaa tcagcattgt ccaaatgaca
    22621 aattttgtta ctttagcaaa agtacttgtt tgtttttttt cacgcattgt tttcaacctc
    22681 gtcattaatt agttgattca agtgtacaat cttttttcca tttccacaag agggcttttt
    22741 tataaaaact tccctttttt taaataatag gtagttacga tacaaataaa taatgttaat
    22801 agaactagcc atataaaggc atcatccctt tcagcaatag gtaacttaat gttcattcca
    22861 tagatactgc cgacgattgt cggtatagtt aaaataatcg ttaaagaagt taaaattttc
    22921 ataatattat tcagattatt cgatacgatt gctgaaaaag tctgattcat ttgttctact
    22981 agttttaatt gaatgcgaga agtagtcatt gcctgctctg tttccaccaa tacatcacgt
    23041 aaatgatttt tcaaagcatg attttcttgg aaaagtttat tttgcgctag ttctttcaat
    23101 gcatctaaat tagcagaagt cgcttctctg aaataaacga ggcttttttg gatatccatg
    23161 atttgataaa gttgtttgtt ttcagtggat acttgaagtt tggcttccaa agtatcgcat
    23221 tgagattgaa gcgtagctaa ataactgttg taagagagtg tgacctgcca aatgaattga
    23281 aagaagatat tgagattagg cgaaatatca ctttttggaa aggtctggct aaagactttc
    23341 tttaaaaaca gtggttcatg attcgtaatt gtgataagct tctttttggg cgtcaaaata
    23401 atagagagcg ggtatgtatc cacttgaaaa taaccgctag gacttgtttt agcaaaagga
    23461 tattgtaaca ataataacgc tggctttgtc aactttgatt gattgaattc ttctgtacgg
    23521 gaattttctg catcgtccaa tatggaagtg atgtaatctg acggaatttc aaattgatgt
    23581 gtcagctgat gaatctcttc ttgattagga cgttctacac aataccagat cgtttggtca
    23641 gtttcctttc cggtattacg cagtttctca ttatctaatg taaaatattt gatcatgatt
    23701 ttcctcctgt tgcctgtttc ttttattata acggataggg aattggaata catggaatgt
    23761 gtttgtaaga tatagaatat aactgcataa atttaaataa aatgaaacag ttgtgtaaag
    23821 tatatacagt atacttgaag caacgataaa aaaggagttt acgttcatgg gcacattcac
    23881 accacttatc ttgttttggt atatttaccc ggtaatcgtt ttgtttggtt gccaatttct
    23941 tgtaaaatta ttttcgttaa atcgtcgttt taaaataaaa gcccctgatt tggcgattcc
    24001 ttttttgtgg ggcggtatgc atgcattgtc tagaaataca gggacaattt ctatcttacc
    24061 gtttttcttt atttctgttt tgctaatggg aatttgtatt gctgttttcc aagcatatta
    24121 ttatgaagaa attatttatc cgcgttattt caagatgact tggcgtttgg tttttctgtt
    24181 gacaatcgtc ttgtacgctg tcttgatcat tgtgaacata ctttcttacc tatgaaaaaa
    24241 atagaaaagc tgtaaaaagt cttttaaaag gctttttgca gcttgatttt taaaaaaatg
    24301 tttatgaata aaaaaagttg ttcccggatt tcccagaaaa ggtggtagaa agtgggggat
    24361 tgtggtagac taattttagt tagtggagga atggggggct ttcaatgttg atgggtgaat
    24421 tccaacataa tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac
    24481 ttggcgaaaa gtttgttctg acacgcggat tggatggctg tcttttcggc tatccgatga
    24541 gcgaatggga aaatctggaa gctaaactga acgaaatgcc gttggctaaa aaagacgcac
    24601 gtacttttgt gcgattcttc tattcggctg ccactgaatg cgaacttgat aaacaaggac
    24661 gaatcaatat acccagcacg ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca
    24721 tcggtgtatc taaccgaatt gaaatttggg atgagacacg atggcaagat ttttctgcag
    24781 cagcagaaga gaactttgac gaaattgctg aaagtatgat tgatttcgga ctataagaat
    24841 aggaagaggc tgactaatga ctgaaacatt tcaacactat acagtcatgt tgaaagaaac
    24901 ggtagatgga ttgaacatca aaccggatgg cgtttatgta gactgtacac tcggcggtgc
    24961 cggacatagt gaatatttat taagccaact aagttctaaa gggcatctgt atgcgtttga
    25021 ccaagatcaa aaagcaatcg atcacgccaa gctgcgttta gcttcttatg tcgaacaagg
    25081 tatggtcaca tttatcaaag cgaacttcag agaattagaa gaacgcttga gtgagcatgt
    25141 agataaggta gacggcattt tgtatgatct aggggtttcc tctccccagc tagatgaagc
    25201 agaacgtgga tttagttatc atcaagatgc accgttggat atgcgcatgg atcagtcagc
    25261 tcctttatcc gcttatcacg taattaatga atattcctac catgaattag taaagatttt
    25321 cttccgctat ggcgaggaga aattttccaa acaaattgct cgtgaaatcg aacgagtacg
    25381 aaaaacaaaa ccaatccaaa caactggtga gcttgtcgaa atcattaaac aagtcatccc
    25441 agctcccgca agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat
    25501 tgcagtaaat gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa tccggttatt
    25561 gaaagtcaat ggaagaatca gcgtaatcac attccattca ttggaagaca gaattgtcaa
    25621 aagtatgttt aaagaatata gtacggtaca agatctacca ccgggacttc ctgtcatacc
    25681 tgacgaattc caaccagaac tgaaagtcat tactcgtaaa ccaatccttc cttcagagga
    25741 agagctaaca gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag aaaaaataag
    25801 aatgaaagag gagtagaatg atggcagaat taaagaaaat gcaagattac gaatatgatt
    25861 tacctgaatt ggtggatcag ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa
    25921 tcattcttcc acaatcaccg gcaaaacgat taaagcagat ttctgctttg gaaaaaattg
    25981 ctgtggcaag cgtggcgatt gctattgttg ctttatgtgt tttaacagtt atgctgcgca
    26041 caaatatcag cggagctgaa aaagaaatca cgtcaataca aacagaaatc aatgataaaa
    26101 agcaagaaaa aacgagttta gaacaagaaa aaaacgaact ttctcgtact gaacggatta
    26161 aacaaatcgc agaagaaaaa ggattgtcta ttaatgacga caatttaagg aaagtgaagt
    26221 aaatgagtct taaaaataaa attcggcggt ttatggaaaa gaaaaatcta aatccgatga
    26281 acaatcgcaa aaaagtaggt atcattttat ttgctacgag tattggattg ttctttttat
    26341 ttgccgtccg tttctcgtat atcgttattg gaggtcatgt tgctggtacc tcattagctg
    26401 aaaaaacaaa acagttgtac caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt
    26461 atgatcgtaa cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt aaagcgatac
    26521 tctcaaaaac atatacctct ggggataaaa aactgtatgt tgaagaaaag aattttgata
    26581 aaatagcaga aatcctgcat aagaatcttt ctatagacaa aaaagatgca ctgaatattt
    26641 tagaggacgg cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat gggaaaaata
    26701 tctcccaaga aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc
    26761 tttattttgt cgaccatcag gctcggatgt atccaaatgg cgtcttttct tctcatttta
    26821 ttggatacgc agttccagac aaagatgaga atggcttagt tggtaaacta ggtttggagt
    26881 ctgcttacaa tgatatcttg agcggtaaag atggtaagat tatttatcaa aaagataatt
    26941 ttcaaaatcc tctgccaggt acagtggcag aagaagaaaa agctgtcgac ggacaagata
    27001 tctacacgac tttagacagc agactacaaa gttatttaga aacactgatg gatcaagtaa
    27061 atgaagaata tcagccagaa gaattgacag cagtcctgat gaaagcaaaa accggagaaa
    27121 tcttggcaat gggacaacga cctactttca atcctgaaac aatggaagga ttgactggaa
    27181 aagatgctat ctggcgtaat ttcttggtac aagatagtta tgaaccagga tcaaccatga
    27241 aagttttcac tactgcagct gctatcgaag aaggagaatt caacgagaat gaaaccttcc
    27301 aatctggaaa aatccaagta gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag
    27361 gtgtgctgac catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg
    27421 aacagcgatt aggcggacga tggtataact atttacaaaa actgggattt ggacaaagta
    27481 ctcattctgg attagatgat gaagtaaacg gtgctttacc tacattaaat attgtcgacc
    27541 gagcaatgag tgcttatgga caagcggtag gtgtgacgaa tttccaaatg atgaaagctt
    27601 tcacttctat tgcgaataac ggtacaatga ttcaacctcg atatatcagc aaagtggtgg
    27661 atccccaaac tggagaagag cgaacaactc aaacagaagt tctagggcaa cctttttcaa
    27721 aagaaacaac agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg
    27781 gtattgctta tggtgtatat agtgtacctg gatacaatgt aacggcaaaa accggaacag
    27841 cccaaatcgc ttctgataca gggggatatc aaacaggaga tacagcctac ctgtattcga
    27901 ttgtggagat ggttccttct gaagatccag actatgtctt gtatctaaca atgaaacacc
    27961 ctaagacata tgatcgaatg gcattggcta aaatcgcaaa tccattgatg aaacgggcaa
    28021 tggattttaa agaaactgaa gaagattcag atacagaaac aaaaacagaa aaagtatccg
    28081 tagccgatta tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac
    28141 tgcaacctgt agtgatcggt aatggtaaaa aagtacaaaa acaatctact gccaacggtg
    28201 atcagttgat ctctggagaa aaactgatcc tatatactgg tggtgataag ctgatgcctg
    28261 acgtaacagg atggtcaaaa gcagatatca tgaagctggg gaaaatcctc ggtattgaag
    28321 ttagttttga tggagatggt tattgtgtca aacaagaact tgctccatat gaaaaaatca
    28381 ctgaagacaa acttagtttt actttagaag aataagaaga gaaataggag agaaaagagc
    28441 atggaatgga cacaagcatt gattcccatt gtcagcagtt gtgcaatgac aattgccgca
    28501 atgccgctat ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag
    28561 ggaccaaagt ggcacaatag caaagccgga actcctacaa tgggcggctt agtgtttctg
    28621 atcggctcta ttttgacagg aatttgggtt ggagcttggc aaaaacagct aactcctact
    28681 cttttcatct tgctttttgt attagcgtta tacggagtaa tcggctttct tgatgatttt
    28741 ataaaaatat tcaagaaaag aaacatggga ctgaactcga agcagaaatt gattggacag
    28801 attctagggg gaatcatctt ttatcttgtt taccgttctg aaggttatcc aggtgtattg
    28861 aatttcttcg gactagatct acctttagga atcatttatg gtattttcgc tctcttctgg
    28921 cttgttggat tttcaaatgc agtaaatctg acagatggca tcgatggatt agttgctggc
    28981 ttaggaagta tctcttttgc agcttacggt ctaattgctt ggcatcagca tcagtatgat
    29041 gtattaatca tttgcttaag tgttttagga ggtttactgg gtttcttcgt ctacaacaga
    29101 aaacctgcca agatttttat gggagatgtc ggttcattag cgttgggcgg acttttagcg
    29161 gcaatttcta taatgctaaa ccaagaatgg acattgcttt tgattggttt gatctatgtt
    29221 atggaaacag caagtgtgat gcttcaagtt acttccttta agcttacagg aaaacgaatt
    29281 tttaaaatgt ctccaattca tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt
    29341 gatacagttt tttggttgat cagcattgtg acttcgttga tcacattatg gatcgtttgg
    29401 taggagatga cgggaaatga aaaaaatatc aacatatgaa aataagaaag tgcttgtctt
    29461 aggactcgca aaaagcggag taagtgcggc caaattatta catgaattgg gcgcacttgt
    29521 caccgttaat gatggaaaac cttttgacga aaatccggaa gcacaagagc tgctttcttt
    29581 agggataaaa gtaataacag gtagtcatcc aatcgaattg ttggatgaag aattttcatt
    29641 gatggtcaaa aatccaggga ttccctacag tcatccttta gtagcaaaag cccaagaaat
    29701 gggtattccg gtaattacgg aagttgagct agcttacgaa gtagcggaat gcccaattat
    29761 tgggattaca ggaacgaacg gaaaaacaac gacaacaacg atgacaggac tactactgaa
    29821 tgcaggagca gatcaaggaa ttgcacgttt agcgggtaat attgggtatc cagcaagtgg
    29881 tgtagcgcaa gaagcaaaat cagaagataa aattgtgatg gaattgtcta gcttccaact
    29941 catgggaatc acagatttcc gccctcatat agcggtgatt accaatatct atgaagcaca
    30001 tattgattat catggtacaa gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa
    30061 catgacagaa aaagattatc tgatccttaa ctggaatcag tcagaactac aagaattagc
    30121 gcaaagaaca aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt
    30181 gtatgctgac gattactcaa tatactataa aaaagaaaaa ataatggaaa tatctgaact
    30241 tggcgtacca ggtaagcata atgtagaaaa tgcactagct gctatttctg tagcaaaatt
    30301 gtatggtata tcaaatgaag ctatacgaga aacgttgcat tttttccatg gcgtaccaca
    30361 tcgtacgcaa tatgtaggag aaatccaagg cagaaaattt tataatgatt caaaagcaac
    30421 caatatttta gcgactaaaa tggctctaag tggatttgaa acgtctaagg tcgtgttatt
    30481 ggctggcgga ttagacagag gaaatacatt tgatgaatta attccttcat taaaaggaat
    30541 caaagcaatg gtcgtatttg gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc
    30601 aggcatcgag acaatagtaa cagccgattc tgtagaacaa gcagttcctt tagcattaga
    30661 aaacagtaca gatggtgatg tagttttatt atcaccagcg aatgccagct gggatcaata
    30721 cccaaatttt gaaactagag gaaacagatt catggaagct gtgaaccgat taaaatagaa
    30781 aaagtagaag cgagcgataa aaaatgaaaa ttttggttac aggcggagga acagggggac
    30841 atatttatcc agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat
    30901 tcatgtatgt gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat acaggaatgc
    30961 ctttccatac cttggagatt caaggattta aacgaaaaat ttccatgcat aacatcaaga
    31021 cggtccgatt gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt gattttcaac
    31081 cagatatcgt tatcggtact ggaggatatg tttccggagc ggtagtgtac gctgcagcca
    31141 aaatgggtat tccaacagtt atccatgaac aaaacagtgt accaggtgtt accaataaat
    31201 ttttaagccg atatgtggat cggatcgctc tttcatttaa agatgcggct tccttttttc
    31261 ctgaaaacaa agcagttctt gtaggtaatc ctcgagcaca agaagtagca gatactaaaa
    31321 aatcagatat tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc ttgatttttg
    31381 gcggtagtca aggagcactt aaaatcaatc aagcagtatc agttttttta agtgaatata
    31441 ttccttcttc ttaccaagtt ttgtatgctt caggagatag atattatgaa gagatcctaa
    31501 ataaaataaa tcctgaaata caaaatatta gtgtccagcc ttatatcaaa aatatggcag
    31561 aggtcatggc ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat
    31621 tcacagctct ggggttaccg gcagtgttgg ttcccagccc atatgttacg aatgatcatc
    31681 agactaaaaa tgcaatgagt cttgttcacg caggagcagc aaaaatgatt gcagataatg
    31741 aactaacagg tgaatcacta agtcagacag tcaacgaaat catgggtgat gaagaattgc
    31801 aaaagcaaat gtgccgagct tctaaagaac aaggaatccc tgatgcttca aaacgtcttt
    31861 atgacttagt aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa
    31921 agaaccaaaa aaattgtctc atccgtcttc tgatgaaaac aatttgactc cttggcaaaa
    31981 ggctaaccga aaatatctgg cagaacaaga gatgaaaaac caagagccaa aaaaagaaga
    32041 cataacatta gatacacccc tttcagattc tgaagaagaa aaaacggttc aagaggaaca
    32101 gcaagaagaa agccagtccg agaaaaaaat cgaatttttt gaagagatca agcaaaagaa
    32161 aaacggtgga ccatataacg gttctttttt gaaccggctg cccaatttaa aaagtcaacg
    32221 gcacaaagta ctatttcgtc ggttagcttt gatcatcact gtattgggta taccgctgat
    32281 ttttttgatc tattatgttt ctccttatag caaattacaa gcagttgcag tatcaggcaa
    32341 taagacagtc aacagccaag aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt
    32401 ttggggacag tattttcatc gatctactta tatcgatcgc cttaaaaaag cacagccaag
    32461 aatcgaaaca gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga
    32521 atataaagag attgctttga tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg
    32581 aacagtatta gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct tggaagagtt
    32641 caaagactct gcaaaaatca aagaattaac aaagcaatac aatcagttat catcagaatt
    32701 acaaaaagca atttctgaaa tcaagtacac gccaagagca agcaataaaa atctgatcca
    32761 attgaatatg aatgacggca atcaagttat tgtaaatatc aataatcttg cgaaccaaat
    32821 gaaatattat tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt
    32881 tggtatcttc tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg gccaatcgga
    32941 agaaacaact gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga
    33001 aacgcaaaaa gcacaaacac ctggatcgtc tagcacaaac ggatcatctg attcaaatga
    33061 ttcaagtgaa acaagtcaaa tagaagataa tccaagtaca actccgtcaa gtttaaatga
    33121 aaattgataa aacaacttta aaaagtaaga atgtactttc taaaaaccag taatttatgg
    33181 tagaattgga agcagtgaat atatctatta ataagtatat tttaaaacgg acgctagttg
    33241 ttcgcttgta tatggaatag taagtaggag ggaatcccat tcatggcaaa aacaggaatg
    33301 tatgtaggcc ttgatattgg aacgacatct gttaaagttg tcgtggctga atatatcgac
    33361 agccaaatga atattattgg agtaggaaac gcaaaatcag aagggattaa ccgaggcatt
    33421 atcgttgata tcgataaaac ggtccaagcg atacaacgag cagtacgaca agcagaagaa
    33481 aaagcaggta tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta
    33541 gaaaattgcc agggaatgat tgcagttaac ggagattcaa aggaaatcac cgatgaagat
    33601 gtcagaaatg tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg
    33661 tcaatattgc cacaagattt tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga
    33721 atgatcggcg taagattaga aatgtacggt ctgttgttta caggacctaa aacgatcatc
    33781 cataacatac gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact
    33841 cctttagcgc tgactgaatc aattctttct gacggagaaa aagattttgg tacgatcgtc
    33901 attgatatgg gtggcggcca aacaacaacg gctgttatgc atgataagca attgaagttt
    33961 accaatcttg accaagaagg tggcgaattt gtcactaaag atatctctat tgtattaaat
    34021 acgtcattta acaatgcaga agcgttgaaa ataaactacg gggatgcata cccagaacgg
    34081 acatcaccag atgaagaatt tccagtagat gtgattggtc agtcagaacc agtgaaagtc
    34141 gatgaacgct acttgtcaga aattatttca gctcgaatgg aacagatttt caacaaagca
    34201 aaagatgcat tggaccaaat cgaagcatta gagttgccag gtggcgtcgt attgacaggc
    34261 ggagctgcta gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta
    34321 aaattgtatg ttccaaatca catggggctg agaaacccgg tatttacaaa tgtaataagt
    34381 atcgtggatt attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg
    34441 ggtgaaacct cagcagctcg tcaaatggta gttgaacaag aaacaacaaa cacatatgag
    34501 agctatgaag cacctgaaga aacaatctac gatgagccgg aaccaaaaga gtccggagaa
    34561 aatgtaaaaa ataaaatcaa gggtttcttt acaaatattt ttgattaatt catcctggga
    34621 ggaataagat atggaattct caattgataa taatatcaat gacggcgcag taatcaaagt
    34681 catcggagtc ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa
    34741 aggtgttgaa ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga
    34801 aacagttatc caattaggcc ccaaatatac tcgtggttta ggtgctggtt ctcagccaga
    34861 agttggacaa aaagcggcag aagaaagtga acaatcctta cgcgaagctt tagacggtgc
    34921 agatatgatt ttcatcactg caggaatggg tggaggaacc ggtacaggtg ctgcaccaat
    34981 cgttgcaggt atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga cacgtccatt
    35041 cacttttgaa ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga
    35101 aaatgtggat acattattaa ttatctcaaa taaccgttta ttagaagttg tagataaaaa
    35161 gacaccgatg cttgaagctt tccgtgaagc agataatgtt ttacgacaag gtgtacaagg
    35221 tatctctgat ttgatcacag cgccaggtta cgttaacctt gactttgctg acgtgaaaac
    35281 agtgatggaa aaccaaggta cagcattgat ggggatcggt gtggctagtg gagaagatcg
    35341 cgtagtagaa gcaacgaaaa aagcaatctc ctctccattg cttgaaacat ctattgatgg
    35401 agctgaacaa gtacttctta acatcacagg cggtttggat atgacattgt tcgaagcaca
    35461 agatgcttca gatatcgtag caaatgcagc aactggcgat gtaaatatca ttctaggtac
    35521 atctatcaat gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga
    35581 tgaatcaaaa aaggaacgca agtctagccg accagctaga caagcacaaa tgcaatcacc
    35641 tgctcaaaag actgtattag atatggatca agctaaacca acttcttcag aagaagaaaa
    35701 cagttttggc gattgggata tcagacgtga acaaaatgtt cgtcctagag ttgatgattc
    35761 caattttgac aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc
    35821 tcatggcgat gatgaattaa atacgccacc gtttttccgt cgtaaaaaat aaaggagagg
    35881 aagagcacat gattgctgac aacttgcaga aaatcaagca agaaattcag gattcgtgtg
    35941 ctcttgttca tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa tctgttgaat
    36001 ccgctcaagc tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag
    36061 ataaattatt agaaaagaaa caagatttat ttgattttcc tgaaataaag tggcatttaa
    36121 ttggtaattt acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat tactttcatg
    36181 cgttggacag tttaagatta gcagaagaaa tccagaagcg gttagagaaa gaattatcct
    36241 gttttattga agtaaatgtt tctggtgagg aatcaaaaca tggaatttca ccagatgagc
    36301 ttttaccgtt tgtggaatct ttagctgcct gcacaaaaat caaggttgtt gggttgatga
    36361 ctatggcgcc taaagaagct tctgatgagc aaatccataa agtgtttggt acgctgaaag
    36421 aattgcgtga tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt gaattaagta
    36481 tggggatgag tcaagacttt caaattgcga ttgaagaagg tgcaacattt atccgtgtag
    36541 gaacagcgct attcaaagac gaataaagga ggaacaaaaa tgccgctttt aaacagagga
    36601 gcaattgcta attttttcgg attagctgat gaggaagagt acgaatacca agattatcct
    36661 gaaacaacaa aaaaggctgt acagccaact aaaaaaaata caacacagaa tcaatcaact
    36721 cattctgtat ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta
    36781 acgcatgaaa gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct
    36841 acaaaaactg tagcgaaaga aagaacagct tcaagaacct ctacacaacc taaagcaaat
    36901 cttgctggaa aaatcactgt aatggaacca agagcttatt ctgaagctat ggcgattgct
    36961 aaacgaatca ttgctggaga agcaacctta gtgaattttc atttagtaga agaaaaacaa
    37021 gcgagaagaa tcgttgattt tctaacagga acagtttatg cactagatgg agatatcaaa
    37081 cgtgttggag atgagatttt tctttgtaca ccgactggga tggagattga cagttcaacc
    37141 gctcaatctt ttgcagatgc gaatttgttt gatctgtaag aggaggaact acgatttata
    37201 ctattgcact attacttcat ttacttcaac gggcagccta tttctatagc attatcttga
    37261 tggtatatgc attgctctct tggtttccag gcgggtatca atcagggcta ggacgattat
    37321 tagctaagat ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg aggattggac
    37381 ccttggattt cacaatttta ggggcaatcc tgatcttgaa cttagccgta caaggtttag
    37441 cacagatcgt tatatggata actgtccatt tagtttattg attgaagcaa caaaatcttt
    37501 attctcagaa tagataggag gggaaaattt tggatgcaaa tgtataccag cactttcggg
    37561 cagatgaacg tacatttatc gattcggtag gcgattggat tgaacaggtt caaggacaat
    37621 atgcacccta tctgaccgaa tttcttgacc caagacaatc ctatatttta gaaacactga
    37681 ttcgacaaag cagtgatttg cgtttcatgt tttatggcgg atatgaacag gcagaaagaa
    37741 aaagatgttt gattttcccc gattattacg aacctgtaga ggaagatttt gaaacagcat
    37801 tgtatgaaat ccattatcct agcaaatttg ccagcctaag tcatgggaaa atccttggaa
    37861 ctttagtggg aacggggatc aaacgctctt actttggcga tatcatctcg gacggtgatc
    37921 gctggcaagt atttattgca aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga
    37981 ttggaaaggt tgctgtacgc ttggaaccaa tcaaatacac agaaatgatt atgccgaaag
    38041 atagctggac acaagaaaga ggaacagtta gttccatgcg actagacagt gtgatctctg
    38101 agatttataa tatttccaga cagcggtcca agcagctgat cgaagctggt aaagtaaaag
    38161 tgaactggac agaaaataca agacctgatt ttgtacttga actattagat attatttcag
    38221 tcagaggtta tggcagagtc cagatccaag aacttgaggg gaagacgaaa aaagaaaagt
    38281 acagggtact gttcggtgta ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt
    38341 aactccatta gatatccaaa ataagacatt cccgacaaaa atgcggggat ataatcaaga
    38401 cgaagtagat gatttcttag atttagttgt tcgtgactat gaggaattga ctcaaagaaa
    38461 tcgagaatta gaaaaagcag tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt
    38521 aaaagatgct ttgaaccaat caatcatcgt agctcaagat acggctgata aagtaaaaac
    38581 aagtgcaagc aaggaatccg aagtgatcgt tacttctgca caaaacaaag cagatgagtt
    38641 agttgcgaac gctgaaaaac gtgcacatca attaacaact gatgcagaag aaaaagcaag
    38701 aaaaatcttg acagatgcaa cagaaaaagc aagacagctt gcgacagaaa ctgaagatct
    38761 taagaaaaaa acacgagtat tccatcaacg tatcagcttg atgcttgagt ctcaattaga
    38821 acaagtgaaa agtccggaat gggatgaaat tttacaacca ttctccagct acgtgactga
    38881 cagccatgaa gtgatcaaag aagttttagc aaaacagctt gacaatgaaa atgatactga
    38941 tgtaaactca gacacaggag tcatagaagc acctatgacg gcatttgata cacaggctat
    39001 tgatatctcg atcgtgcctg gtgaaaatga aaacgagtag aacagaaaag cactttgtta
    39061 ttcttaaagc gagccgatga gagtggaagt tcggtaagac actgcatggt gaaagatcct
    39121 ctacttgaga cctgcttttc cgaaattaga gtaagaagca gcggctgacc tcgttattgg
    39181 tcacagagag tttgcactag atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg
    39241 tcccttgcgg ctgaacatgc ttttttgtgt ccggaaaaca aataaaggag tgtatggttg
    39301 aatgaaaatg aaagaaacct tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt
    39361 gccaaaccga gaaggcgagt ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag
    39421 acaagagttg aacgaaggaa aacctagctt tgttcttcat gatggacctc catatgccaa
    39481 tggtaatatc catttaggac attcattgaa taaaatcagt aaagacatta tcattcgttc
    39541 aaaatcaatg tctggctatc gtgcaccata tgttccagga tgggatacac atggattgcc
    39601 gattgaacaa gtattggcta ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata
    39661 cttggaaaaa tgtcgtgaat atgcactttc ccaagtagat aagcaacgag cagatttcaa
    39721 acgcctagga gttgcaggag attgggaaca tccttatgtt acattagatc cttcttatga
    39781 agctgcacaa gtacgcgtat ttggtaaaat ggctgaaaaa agctatattt acaaaggatt
    39841 gaaaccaatt tactggtctc cgtcaagtga gtcttcttta gcggaagctg aaattgaata
    39901 taaagatgta aaatctccat ctatttttgt agctttcaat gtcaaagacg gcaaagatat
    39961 tcttgatgaa gatacagctt ttgtcatttg gacgacaaca ccatggacat tgccagcaaa
    40021 ccaaggtatt gctgtaaatc caaaatttac gtatgtattg gtggaagccg atggccgtaa
    40081 gtttgtcgta gcgaaagatt tgctagaaac agtccaatca gaaatcggtt gggaaaatgt
    40141 aacagttcta aaagaatttg ctggacaaga aatggaatat atgactgcaa gtcatccatt
    40201 ctatgaacgt gaatcacttg tgattttagg cgatcatgtt actttagacg ctggtactgg
    40261 attagtccat actgctccag gacatgggga agatgactat atcgctggta gcaaatataa
    40321 actatctgta gtgtcacctg tagactcaaa aggattgttt acagaagaag cacctggatt
    40381 tgaaggtatc ttttatgaca aagcaaatcc tatgatcact gatctattaa aagaaaaagg
    40441 agctttgctg aagctagatt tctttactca tagttatcct cacgattggc ggacgaaaaa
    40501 gcctgtgatc tatcgggcaa ctccacaatg gtttgcatct atcgataaat tccgtcagaa
    40561 tattttggat gaagtagaaa aagtagagtg ggttatccca tggggtaaaa cacgtttgta
    40621 taatatgatc cgtgaccgcg gcgattgggt aatttcccgt cagcgtgcat ggggagtacc
    40681 acttccgatt ttctatgctg aaaatggaga agcaatcatc acaccagaaa caatcgaaca
    40741 tgtggcaaaa ttatttgctg aacatggatc aattatctgg tttgaaaaag aagcgaaaga
    40801 acttcttcct gaaggattta cccacccagg ttctcctaat ggcgaattta caaaagaaaa
    40861 agatatcatg gatgtctggt ttgattctgg ttcttctcat gaagctgttt tacgtcagcg
    40921 accagaatta tcattcccag cagatatgta tttagaagga tcagaccaat atcgtggctg
    40981 gttcaactca agtatcacga ctagtgtggc tatcaatggt gtagcaccat acaaatcagt
    41041 tatttctcaa ggatttgctt tagatggtga aggccgtaag atgagtaaat ctttaggaaa
    41101 catcattgca ccagaaaaag tgatcaaaca atttggtgca gatatccttc gcttatgggt
    41161 atctagtgta gattatgaag cagatgttcg tgtttctatg gatatcttaa gtcaagtggc
    41221 agaagtatat cgtaaaatca gaaatacgat gcgtttctta ttagcgaata cagaagattt
    41281 cgatcctaaa aaagatactg tttcgtacaa tgatttacgt tctgtagaca aatatatgac
    41341 cgttcgattg aaccaagtaa tcaaagaaat ccgagaagaa gggtacgaca aatataactt
    41401 catgcatatt taccgtacag ttatgaactt cttgacggtt gatctatcgt cattctacct
    41461 ggattttgct aaagatgtag tttatattga agcagaagat gattatcaac gtcgttgcat
    41521 gcaaactgtt ttctatcaaa cagctgtagc tttgacaaaa ttattgactc ctatcattcc
    41581 gcatacagca gaagaaatct ggtcattcct gaaagaagaa gaggagtacg ttcagttaag
    41641 cgagtttcct ggttatgaag agtttgcaaa ccaagaagaa ttgatggata cctggactgc
    41701 tttcatggac ttccgtgata atgtcttaaa agcattggaa gaagcaagaa acagcaaatt
    41761 gattggtaaa tctttggaag caaaactaac tgtttatcca aaacaacaag ttcgtgaaat
    41821 gttaaaagct ttggatgcag atattgcaca attattgatc gtttcaccag actactttga
    41881 agttatggca gcagatgaac aagtgccaga taacgctatg gtatttgatg atgttgctat
    41941 tacagtggaa aaagcagacg gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt
    42001 aggagtagat gaaaaacttc ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt
    42061 ctatcctgaa gctgtagctg aaggattcga agaaaagtaa aagggaaaag ggggtgtgac
    42121 atgttctttg tcacaccccc ttttgttatc aatcgtttaa ttttccacgt tcacgatacc
    42181 agttatctgg ttgccagatc cattcgaatc cgtctttttc aagtagttcg aaagctgctt
    42241 taggtcccat tgatccagct ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt
    42301 ttcgaatgac atccacgata tgccaagatt gagcgacttc ttcccaatga gagaagttcg
    42361 ttccatcacc attcaacgca tctaatagta atttttcgta agcttctggg ctattttcaa
    42421 ccatttctgc actatttcta aattccaatt tgattggttc cgtttcaaat ccttgcccta
    42481 cttctttccc attcaaggat aaagaaaatc cttcagttgg ctgaatatag atcgttagaa
    42541 cattaggtgg aagtgtcgta tcgtcacatg gttcatcgac agatgttttg aatacattaa
    42601 caggaacttg tttgaatacg atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg
    42661 tacgtacata gaaaggaaca cctgaccaac ggaagttatc gatagtgaat ttgcctgcga
    42721 cgaatgtttc agtctctgat tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt
    42781 gtacaccatc caattcacct tgagcatatt gcccgcgaac gaagttttct aacacttctt
    42841 cttcgttata aatgcggata gctttcaatg ctttgatctt ttctgtacgg atttcttttt
    42901 cggaaaatgc tactggcggt tccattgcaa gtaatgctac aacctgtaaa atatgatttt
    42961 gcaccatgtc tttaagtgcg ccactatgat cgtagtagcc cccgcgatct tctacaccga
    43021 ggttttcgcc aaaagtaatt tggatattgt cgatatagcg attgttccat tgagcctcaa
    43081 aaatattatt ggcaaaacgg atagctgaga tgttttggat catttctttt cctaaataat
    43141 gatcaatcct gaaaatatct tgttctggga atacttctct gatttgctgg ttcaattcaa
    43201 aagcagactg gtaatcagaa ccgaacggtt tttctatgat caaacgattg aagccatttt
    43261 ttgtcataat attttgtgat ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca
    43321 tagccaaata atagacacgg ttaccgttta attgatagtg tccatctaat cggtcagaca
    43381 attcttttaa tgtctgataa tgcgttgaat catttacatc atgggattga tagtaaaaat
    43441 ggcttgcaaa ggtagttgct tcttcatctg aaggattcaa tgcacggatt gtttctttga
    43501 caacttcacg gtagtgctca tcgctccagg gacggcgagc ggttccgatt acagcaaaat
    43561 gctcgcctaa atttcctttt ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg
    43621 ctaggtcacc tgtaccgccg aatatcgtaa acaatacatt tttttcatta gacatgtgga
    43681 tacactcctt tatattttat cgacaaaaat cgtgctggcc gccttaaagc tgacggggat
    43741 tttctttcta tcattttgga tagtgatcgg tccttcataa gctgcgatgt caacgatctc
    43801 atattcttcg ttgatattca ggtcaatagt gactaagtaa tccaacaatt ctttttcatc
    43861 aagcactcgt gcgatacgca cttttgtacc gacaggatag gactccaatg tttgtcggcg
    43921 ttcttcatgg acaacttcct cttttttagg aatcgatcct ccatgaggac aatgttcagg
    43981 atgattcaaa tagttttcta aatgatctgc aagatgttcc gaagtcacat gttccaatac
    44041 ttcagcatca tcatggactt cattccagct atagtctaaa tgttcaacta aaaaaacttc
    44101 ccataagcga tgtttgcgaa tcagactgct agctttttgc agtccgcttg cagtcaattg
    44161 tacaccttga taaggggaat gttctacaag ctcttctttc acaagtttag aaatcatttc
    44221 actgacagat gcagctgaga catctagact agatacgatt tgtttattgt tgactttcgt
    44281 tgtatctcca cctagttcta aaatgatttt caaataatct tctcgatttg gtgtcatatt
    44341 cgatcatcct ttcatggtaa aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt
    44401 gactattttt ctaaataaaa aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa
    44461 gatgatagag aagactcatt ctttcgttta taaaggatat atatatacat tgggagggat
    44521 ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc
    44581 gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc
    44641 tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt
    44701 aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga
    44761 gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac
    44821 ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga
    44881 gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa
    44941 aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata
    45001 catagtagga agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta
    45061 ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg
    45121 cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta
    45181 ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga
    45241 agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt
    45301 tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag
    45361 taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat
    45421 ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc
    45481 ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg
    45541 ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag
    45601 aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc
    45661 tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg
    45721 atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact
    45781 taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa
    45841 aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt
    45901 ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc
    45961 atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag
    46021 caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa
    46081 tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa
    46141 atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa
    46201 tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag
    46261 aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt
    46321 accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa
    46381 gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg
    46441 atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg
    46501 actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc
    46561 gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg
    46621 aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt
    46681 atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa
    46741 atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca
    46801 agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct
    46861 tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc
    46921 tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg
    46981 tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga
    47041 tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc
    47101 caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc
    47161 aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat
    47221 ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat
    47281 tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc
    47341 ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga
    47401 ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga
    47461 tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca
    47521 actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag
    47581 tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa
    47641 aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct
    47701 tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg
    47761 aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct
    47821 aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata
    47881 cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa
    47941 aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat
    48001 aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata
    48061 agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt
    48121 tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg
    48181 acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta
    48241 agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct
    48301 ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact
    48361 tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct
    48421 gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg
    48481 aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca
    48541 gatgcgccat ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt
    48601 ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt
    48661 ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg
    48721 gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa
    48781 agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa
    48841 agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct
    48901 tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat
    48961 cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat
    49021 gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt
    49081 ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt
    49141 caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg
    49201 gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa
    49261 gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc
    49321 gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat
    49381 ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat
    49441 gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt
    49501 tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg
    49561 agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg
    49621 gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt
    49681 ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag
    49741 cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca
    49801 agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg
    49861 ccactggtta tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat
    49921 caagagcatt tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc
    49981 agtgatttga tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc
    50041 atgattgtga tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat
    50101 caaaatatcc agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct
    50161 gaaactttac agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct
    50221 gccaaattat atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag
    50281 atgcagtttt tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat
    50341 gagatcaaag caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca
    50401 gcctatgacg tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag
    50461 atcccgacag ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt
    50521 gatgaactga tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt
    50581 atcgtggaac ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga
    50641 ttgatcaaac aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat
    50701 cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta
    50761 aaaattccag aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa
    50821 aaatgaacac actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa
    50881 gagctaaaaa gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt
    50941 tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt
    51001 agtttgttca gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga
    51061 agaaaaaggg atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt
    51121 agaatctaag aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca
    51181 atttgaacaa aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta
    51241 tggcatgatg aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa
    51301 gtatgcagtt gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg
    51361 ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa
    51421 atcggtgatt ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa
    51481 gcgcatcatg cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg
    51541 acattattga ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta
    51601 gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt
    51661 tgaacggaag aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct
    51721 ttatataaaa aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga
    51781 aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg
    51841 atgcttttat cggcacactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg
    51901 caattattcg tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag
    51961 acaaaggaat ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg
    52021 gtacactagc aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag
    52081 cttatggagt aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta
    52141 tttcctttgg tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag
    52201 atgcaatcaa tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat
    52261 tatggggttg gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca
    52321 tgggagctat ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa
    52381 tgcctgattc agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga
    52441 tcgctttata cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac
    52501 tgatcattga tttggtgcaa aaatatattg cagaaccatt cctaggatta tctcaagggt
    52561 tgggagcagt attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg
    52621 gatcaaatgt attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata
    52681 ttgatatttt ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa
    52741 tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg
    52801 gcggatcagg cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag
    52861 attatctaac agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag
    52921 tgatgtttgg tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc
    52981 cagtagtagc aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat
    53041 cgcagcaagt cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg
    53101 attggcgggc accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac
    53161 catttgtcat ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac
    53221 gtgaggagcg ggacgcttta agtttcgttc ctcataatga attttcgagg aggaactgaa
    53281 atgattacaa ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac
    53341 aatgaagaag gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa
    53401 acaagtgcag aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag
    53461 ccttggggaa cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat
    53521 aggatggttg agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg
    53581 ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt
    53641 tttctaacag gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag
    53701 gagaaaaata actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg
    53761 ataaagttat ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta
    53821 gaaaacagct gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca
    53881 taaaaatcga agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga
    53941 atgaattcgt cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt
    54001 acacagaaaa aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa
    54061 caatcttgat gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa
    54121 cgtattaaat caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag
    54181 gaatttttca taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag
    54241 gggcagcaaa atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt
    54301 tgatttagcc acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg
    54361 tacacttgcg gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag
    54421 tcaagtcatg gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg
    54481 cattggctat gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat
    54541 ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt
    54601 ctcctatccc ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca
    54661 actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga
    54721 ttttgaagat gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg
    54781 agctggtctg aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta
    54841 ccagtttgct ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct
    54901 tgcaacgtct tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag
    54961 ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg
    55021 ctacggcggt aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac
    55081 accttatatg atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa
    55141 aaagagacaa gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga
    55201 aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac
    55261 aaaactccca atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg
    55321 taaagtggaa gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac
    55381 gttaagctca atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac
    55441 gggtgatgac aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa
    55501 tgttaaagta aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg
    55561 ttttcttttt tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat
    55621 gaatctaagc gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc
    55681 cctacattat ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt
    55741 gcctaactta taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct
    55801 acaattgatt tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag
    55861 cgataggtcg ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgcg
    55921 gtacaggggt ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata
    55981 taaactatcg aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat
    56041 atgaagataa gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct
    56101 aagtatattt actccttgat aaagtaaaat agatgcatgt gtatgcctta gtccatgcaa
    56161 agtaaattgc ttttcaagcc ctaattcttt taattttttg cgcaaatatt tgctgacggc
    56221 gtttgatgaa actaatccat tttttaagtt gaaaaaaaca aaattatgag ggtttttgat
    56281 ttcaaaattc tcgaataatt ctttttgatt gattttaaac ttttttaaga gatcaatcgt
    56341 atgtttatca atggaaattt tcctatttga agtttcgttt tttgtttttc cccattcatt
    56401 taatttataa ttccatgttt tattaatagt gatgatttga tcttcgaaat cgatatcttc
    56461 ccatgttaat cctagaagtt cagcaaatcg cattccagtg gcaccagcaa ctaacacgag
    56521 cataggagaa gagtactgcg cagaaaggtt ttcttctacg agtttcatta aagatttaaa
    56581 ttgatcataa tctaaatatt tatcttcttt tggcttcaat gaatcttttc ctttgattac
    56641 tgcttttcta gttggatcaa acggaattaa tccttcttct acggcatctt tcaacgacgc
    56701 tctaatatgg ttattaaact taataacggt agattttaca tggtctttag catacttatt
    56761 taaaaagcgt tgatatccca ctctatctaa atcagaaatc aataccgctg gcatatattt
    56821 ctttatgttc attaacgtat cttcgtattt tctataagtg ataggagata ctgttccttc
    56881 tttataaagt tgcatccaat cctcaaagta atctgataat aatagatttt tgcgatcccc
    56941 tttaaggcct ttgttcaagt tatattctaa ttcgttggcg gcatcttttg cttctccttt
    57001 tgtcttaaat cctgattttc ttatcttcgt gtattttccg tcatcttttt tgtatgaaat
    57061 ttcgtattgc caactatttc cacgtttgac taatcttgcc ataattgaat cagctctctt
    57121 tctctgatac aataggaact ataaagaagc ccattgtttg ggttttatta ttttttgcac
    57181 atcctttttg cttgccggcg caaggatgtg ctatttgtcg ttttaatggt ataattggat
    57241 gtgtattaga tcatccaatc agaggtgttt attatgatta aagtgatttt taaaagaata
    57301 actgaacttc cagagtttag attatttgtt gattattggt atgtctggtt ttttattgtt
    57361 gcattgctga taggtctaga tgtcatatta gaaagaaaac ttaagtaaga gttgagttaa
    57421 taaattgaat tcctaaatga gatattttgt agcccatatt ttgtttgccg acatcaccta
    57481 atactatgga gctaatcgtt ttttctaaca gaccaaaatc agataatcta tttaagctac
    57541 catctattag ctcttgtgaa tagccagaca tgctgatatt tccagtgaaa ttttcgtttc
    57601 cacgactcat taagcatgct aacaattgaa tctcaatcga tgttaattga ttcagtacaa
    57661 gaagataata ctcctcgtta tcccatgaag cgttgttaat tcgaagtaaa caattcttat
    57721 aaagattttt atacatatct attttatttt gtgcctttga tttttctacc tcgtcattta
    57781 tcccttcgaa tattccaatg agttggtcct tgttctctaa gttgaaaaca tcttctggaa
    57841 tttgatcgcg aatgttttct aatgattcgt ttaaattttt gtagaatttc tcgatgcgtt
    57901 tgaaacgctt ctcgttttta gacccaaaat aaagtgactg cacaacgcca ccaatataag
    57961 gaattgctcc aataccgcct tcaactatta attcaagata ttcttttttg gttagttttt
    58021 catttctatt atccatagtt acctcctatt ttgtcatttc ctttacaagc attgtgactc
    58081 ctgatggcac tcttttgtat aggttttatt attgtgcaca atctaactct tagtggggcg
    58141 tggttcattt attttttact tactttacta ttaatcatat cccattgagc ataggatttt
    58201 tctatgttcg atcctcttga actaatttct cctgtaaaag ttactttatc gccctttgta
    58261 aaggtatcag cattgtttaa gtccttcgca aaaattacat atgaaacttt aggtgttgca
    58321 gaaatatcat tccaactttt atcatcgtac tctttgtctg ctataatggc tattctagta
    58381 gtcattgatt cgataacagt tccttcaaaa gtatatgttt taccactaat atatttagta
    58441 aaagctgtat tttgctcagt agctggagta atagaataat aactatctaa gaaagcaccc
    58501 cagtcatgat tatttgtctc taaaaattct tcaatttgag ttaggctttt attgctgctg
    58561 ttagatgtag tagtttgagt atcagatgat gagctttcct gtttgttatt gtcatttgcc
    58621 tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa taatcccgat aataaggaga
    58681 atcataggtg ttttcttatt tgtttttttg ataaatctta caattaacat gattaaacct
    58741 ataataaata aaacaatacc agtaaaacca aatataccgt ccattaatag atcctctttc
    58801 tcttttgata ttgaatatta taaaccaagc aattcttttt tcttaaaatc gaattcttct
    58861 tgattaataa tcccttcatc taacaactct ttatactttt taatttcatc tgcacttgaa
    58921 atttgtgttt ctttggaatt attatctaat atagattgtt gcttctctat atattttttt
    58981 aaattttcca tttcattcca atattttttt gtaaacataa cggtgttttc atctttcgtg
    59041 gccgctaata tacctccgcg actttcgttt ccgcctagca aggtaaattg tatataacca
    59101 ttactcatac ctggtttttt taattgtacg gctgatatat ttttaaaggg gatagttttt
    59161 tctcctttaa taccttgatt aactaaattt ataaatcctt ttctaataat tgaaatagaa
    59221 tcatcagtta ctttaatcaa ggtttttcct ggagatttta ttaaaatctc tttttccatt
    59281 ttttcttcct catttctgtg atatgatttt ttttgtagaa gctcatatat gaggaaagag
    59341 cccgtgttgc agcacgggct tttttaatgt tttgctgaaa tcggtttttt taataattct
    59401 tgatgtattc atacatattg ccttgcgtaa gaatattttt tcttaaaata gcattggcag
    59461 acagcataac aagtgtatct gagcttattc tgaatagaat aatactccat aaatttttcg
    59521 agattaaact gagattcatc agtcagttcg ttctcaatat agatatttaa taaaattaga
    59581 atagctattt tatctgcttc tgtttcaaat tttgagtgaa aagttgtaga agtatcgtac
    59641 aaaactgaaa attcaaaatg agaggcgctg aaatgtgcaa gttcatgaga taaatgaaag
    59701 gcttctgcag tttcaccgta tagattttca ttcaaaaaaa tgattctggg ttttggatag
    59761 tagaaaccag gctctttcat ctccatataa actactttca aattgtattc gctcaacatt
    59821 tctttcaatt tcaaatacat acaaaccatc actccaacta ttcattttcc tctaaagctt
    59881 tagcaattgc aatcgcttta cgcattgtct ccttagatat ttcttttcca tcaaaagaaa
    59941 aaacagtatc gtcttctgat aaatccacat gtttaggggt ttctctttct tctctaccta
    60001 gaaggtagtc tacagagaca tcgaaatagt cagcaatccg acttaattca tctgaattag
    60061 gagtattatt tttccacttt gctagataac cgttagaata tccaaaatta atttctaact
    60121 gccgtataga tatctttttc ctcttcgcca agtcttttat tatttcatag gtattcatcg
    60181 ataaaacaac ctttctaaat gtttacaaaa aaagtttaga aaaatacgca gaaataattt
    60241 gactaattct gagtaatgag ctatactata tcttgtaaac agatttaaca actaaaaaga
    60301 caacaaaaaa cactattgat atgtaaatgc agaccgccaa gaaagcttta aaatcaatgt
    60361 ttttatgtct tatttaatta tgctcttagt atagagtatt actcagatac tgtcaattga
    60421 atttagaaaa aagttgttaa atttgtttac gaatataaaa gaaaggaatg agaaatatga
    60481 acacaccgca aatttttaat ttcgaacaaa acgaagttcg gacaatttta gtaaatgatg
    60541 aaccatattt tgtaggcaaa gacgttgcga gtgttttggg ttattcaaac actaaagatg
    60601 ctttgtcgcg gcatgtagat ttggaagata agatggggtc gcgaatcacg acctcaggtc
    60661 aatcaagaga gatgacaatc atcaacgaat ctggtttgta cagtttaatc ttaaaatcaa
    60721 aacttccctc tgccaaaaaa tttaaacgtt gggtaacaag tgaagtgttg ccagcaatta
    60781 gaaaacatgg aggttatcta actccagaaa aagtagaaga agctttgctt aatccagata
    60841 caatcattca attagcaact caactaaaag aagaaagaac tggaagatta atcgcagaac
    60901 aaaagattgc agagtacgaa cctaaaatct cctatttaga tagcatatta tcttctacag
    60961 attcagtaac aattagtcag attgcagcag attatgggat gtctccacaa cagatgaata
    61021 aattacttca taaactaggt attcagaaaa aagtcggtaa ccaatggtta ttgtgcaaaa
    61081 aacacatgaa ccaaggatac acaaaatctc atacaactga gatcccgaaa gccgatggtg
    61141 gcactaaaat tgtaatgaat accaaatgga cacagaaagg gcgtctattt atctacgaat
    61201 tactaaaaaa agaaggatat taccctcaaa tggatttaga ggaaattggt tagaaaggag
    61261 ttttagtatg actgacattg cagaaatcac tcaacgagat agagaaaaaa tcaaagaata
    61321 tgtcgaaagt tcaaagttct taacttacac catgcttgct gaaagatttg gaattagcaa
    61381 aagctactta tctttaattt taaacggtaa aaagacttct gcagaagcaa acagaattat
    61441 agattcgatt atcactatgt acgaattgta aagggaggaa cagctaatgc aatatctaga
    61501 agcaaaaatc ccaattccag aaggctatgt aattatctcc caagtggatt atgaggagtt
    61561 aaaaaaagct gatgatactg gtagatggat gacgttgcca gaagtgctag aacggattaa
    61621 cagaaaatat gattggttta cttctagagt tttaaagaac ccaagatata gaaatattat
    61681 cgatatcgag aaaaacaaaa atggatttgt ctattatcca gttgaaggaa gagacacata
    61741 tctattttta agaagtaaaa cacttgaatt tttagaaaca aatttttcgg aaatcttaag
    61801 gaggcaagcg gatgggaaaa tttaatagag cgctagtgtt cagcgcaccg ctaatcatct
    61861 acgctttagg actttgggga agcag
//
LOCUS       DAFCNS010000004        61373 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFCNS010000004 DAFCNS010000000
VERSION     DAFCNS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.004"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..563)
                     /locus_tag="LNE23_000256"
     CDS             complement(<1..563)
                     /locus_tag="LNE23_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBK6930137.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="LNE23_000257"
     CDS             complement(750..1400)
                     /locus_tag="LNE23_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930138.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LNE23_000258"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LNE23_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBK6930139.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="LNE23_000259"
     CDS             complement(2912..3910)
                     /locus_tag="LNE23_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBK6930140.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LNE23_000260"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LNE23_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBK6930141.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="LNE23_000261"
     CDS             complement(4926..5657)
                     /locus_tag="LNE23_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBK6930142.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="LNE23_000262"
     CDS             complement(5661..6926)
                     /locus_tag="LNE23_000262"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBK6930143.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="LNE23_000263"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="LNE23_000263"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBK6930144.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="LNE23_000264"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="LNE23_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBK6930145.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="LNE23_000265"
     CDS             12268..13770
                     /locus_tag="LNE23_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBK6930146.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="LNE23_000266"
     CDS             13772..14509
                     /locus_tag="LNE23_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6930147.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="LNE23_000267"
     CDS             complement(14554..14727)
                     /locus_tag="LNE23_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930148.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..15368
                     /locus_tag="LNE23_000268"
     CDS             14703..15368
                     /locus_tag="LNE23_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930149.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="LNE23_000269"
     CDS             complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="LNE23_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBK6930150.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="LNE23_000270"
     CDS             complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="LNE23_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBK6930151.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="LNE23_000271"
     CDS             complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="LNE23_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBK6930152.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="LNE23_000272"
     CDS             complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="LNE23_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBK6930153.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20581..21747)
                     /locus_tag="LNE23_000273"
     CDS             complement(20581..21747)
                     /locus_tag="LNE23_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBK6930154.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21763..21990)
                     /locus_tag="LNE23_000274"
     CDS             complement(21763..21990)
                     /locus_tag="LNE23_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBK6930155.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22474..23064
                     /locus_tag="LNE23_000275"
     CDS             22474..23064
                     /locus_tag="LNE23_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBK6930156.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23254..23967
                     /locus_tag="LNE23_000276"
     CDS             23254..23967
                     /locus_tag="LNE23_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930157.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24082..25092)
                     /locus_tag="LNE23_000277"
     CDS             complement(24082..25092)
                     /locus_tag="LNE23_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBK6930158.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25129..25611)
                     /locus_tag="LNE23_000278"
     CDS             complement(25129..25611)
                     /locus_tag="LNE23_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBK6930159.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25761..26129
                     /locus_tag="LNE23_000279"
     CDS             25761..26129
                     /locus_tag="LNE23_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBK6930160.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26594..27046
                     /locus_tag="LNE23_000280"
     CDS             26594..27046
                     /locus_tag="LNE23_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930161.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27170..28021)
                     /locus_tag="LNE23_000281"
     CDS             complement(27170..28021)
                     /locus_tag="LNE23_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBK6930162.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28034..28819)
                     /locus_tag="LNE23_000282"
     CDS             complement(28034..28819)
                     /locus_tag="LNE23_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6930163.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="LNE23_000283"
     CDS             complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="LNE23_000283"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBK6930164.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30116..31039)
                     /gene="truB"
                     /locus_tag="LNE23_000284"
     CDS             complement(30116..31039)
                     /gene="truB"
                     /locus_tag="LNE23_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBK6930165.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31407..33769)
                     /locus_tag="LNE23_000285"
                     /pseudo
     CDS             complement(31407..33769)
                     /locus_tag="LNE23_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34217..34390
                     /locus_tag="LNE23_000286"
     CDS             34217..34390
                     /locus_tag="LNE23_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6930166.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="LNE23_000287"
     CDS             complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="LNE23_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBK6930167.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34921..37227)
                     /gene="infB"
                     /locus_tag="LNE23_000288"
     CDS             complement(34921..37227)
                     /gene="infB"
                     /locus_tag="LNE23_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBK6930168.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37240..37551)
                     /locus_tag="LNE23_000289"
     CDS             complement(37240..37551)
                     /locus_tag="LNE23_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBK6930169.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37548..37841)
                     /locus_tag="LNE23_000290"
     CDS             complement(37548..37841)
                     /locus_tag="LNE23_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBK6930170.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="LNE23_000291"
     CDS             complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="LNE23_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBK6930171.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="LNE23_000292"
     CDS             complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="LNE23_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBK6930172.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39679..44037)
                     /locus_tag="LNE23_000293"
     CDS             complement(39679..44037)
                     /locus_tag="LNE23_000293"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBK6930173.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44249..45958)
                     /locus_tag="LNE23_000294"
     CDS             complement(44249..45958)
                     /locus_tag="LNE23_000294"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBK6930174.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="LNE23_000295"
     CDS             complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="LNE23_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBK6930175.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47455..48255)
                     /locus_tag="LNE23_000296"
     CDS             complement(47455..48255)
                     /locus_tag="LNE23_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBK6930176.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48252..49064)
                     /locus_tag="LNE23_000297"
     CDS             complement(48252..49064)
                     /locus_tag="LNE23_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBK6930177.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49259..49816)
                     /gene="frr"
                     /locus_tag="LNE23_000298"
     CDS             complement(49259..49816)
                     /gene="frr"
                     /locus_tag="LNE23_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBK6930178.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49819..50541)
                     /locus_tag="LNE23_000299"
     CDS             complement(49819..50541)
                     /locus_tag="LNE23_000299"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBK6930179.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50677..51558)
                     /locus_tag="LNE23_000300"
     CDS             complement(50677..51558)
                     /locus_tag="LNE23_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBK6930180.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="LNE23_000301"
     CDS             complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="LNE23_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBK6930181.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52798..53277
                     /locus_tag="LNE23_000302"
     CDS             52798..53277
                     /locus_tag="LNE23_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBK6930182.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53494..54585
                     /gene="phnW"
                     /locus_tag="LNE23_000303"
     CDS             53494..54585
                     /gene="phnW"
                     /locus_tag="LNE23_000303"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBK6930183.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54578..54706
                     /locus_tag="LNE23_000304"
     CDS             54578..54706
                     /locus_tag="LNE23_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBK6930184.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54710..55255
                     /locus_tag="LNE23_000305"
     CDS             54710..55255
                     /locus_tag="LNE23_000305"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBK6930185.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55709..57400)
                     /locus_tag="LNE23_000306"
     CDS             complement(55709..57400)
                     /locus_tag="LNE23_000306"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBK6930186.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="LNE23_000307"
     CDS             complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="LNE23_000307"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBK6930187.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58882..59901)
                     /gene="argF"
                     /locus_tag="LNE23_000308"
     CDS             complement(58882..59901)
                     /gene="argF"
                     /locus_tag="LNE23_000308"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBK6930188.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="LNE23_000309"
     CDS             complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="LNE23_000309"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBK6930189.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT        17059 a        13104 c        10625 g        20585 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt ttgttggtac
    15361 aaacatgaca actacaaaaa aaccaggcga acatatgatt gttcgtctgg tttttaatta
    15421 ttaatgactt agcgttttta ctagaaaaac tacttccgct ttttcccgcc aaaagcatct
    15481 ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa ctttgatgcc gccagcttca
    15541 gcgaatgcac gtaatgcttc tctttgttgt tcatttagat ttttcggtgt gatcagtttt
    15601 actttgacat gttggtcgcc tgttgcacca ccacgtaact tcggagcgcc ttttccacgt
    15661 aaacggaagt tcgtacctgt ctgtgttcca gcagggatct tcaatttcac atctccatgg
    15721 acagttggta cttggacttc atcccccaaa gctgcttgta caaaacttaa aggcaattca
    15781 tagtaaattt cagaaccttc acgatcaaag atatcacttt cttctacacg gaagaccaca
    15841 tacaaatcac caaatggtcc gccgttttct ccagcttctc cttgaccagc taaacgcatt
    15901 tgttgtcctt cttctacacc agctggtaca ttcactttaa ctgaatgtgc tttcttctca
    15961 tgtcctgagc catgacaggt tggacaaggt tctttgattt ctttccctgt cccatgacaa
    16021 acatcacaag tttgacggct catcactcgt ccaagtggtg tttggcgttc tacattaatc
    16081 gtacctgcac catgacattt atgacaagta gttggttgtg tgcctggttt tgcaccgttc
    16141 ccaccacaag tatgacagat ttcttcccga ttatacttga tttctttttc tacaccaaaa
    16201 atcgcttctt caaaagacaa gtcgaccgta tattgcaaat cagcaccttg acgaggcgca
    16261 tttggatcga ctgaccgacc gcctcctcca aagaaagagt cgaagatatc ttcaaaacca
    16321 ccgaagccgc caccagagaa gccgccgaat ccaccaaatc cgccaccagc tccaccagca
    16381 ccgtaatttg gatctgtgcc tgcatgcccg tattggtcat atgcagcgcg tttttgcggg
    16441 tcacttaaaa tctcatatgc ttctgatact tctttgaatt tttcttccgc atcaggttct
    16501 ttattgatgt caggatggta ttttttcgaa agcttacgat aagctttctt tatttcatct
    16561 tctgatgctc cttttgagag ccctaagact tcataataat cacgttttgt tgccataggc
    16621 tttcctccta ttggttctca tcattttcac gtcgcttgtt atcatatcat attttgtttg
    16681 aaacaaaaac cgatacttca aactattttt caaaagaaaa agccaaagta gcaactttgg
    16741 ccttctctcg aaattcagtg aactgattat ttatcgtcgc catcgacttc ttcaaaatca
    16801 gcatcaacga catcatctgc tccgccttga gctgcttctg ggttttcttg agcttgttgt
    16861 tgtgccgctt gttcgtatag tttaacagtc aagttttgaa cgatttcgtt caatgcatcg
    16921 cgtttagttt tcatttcttc gatgttgttt gcttcaacag cggctttcaa ttcatcgcga
    16981 gctgtttctg cttttttcac ttcttcttcg tccactttgc cttctaattc tttcaatgtt
    17041 ttgtccacag agaatagcaa tgcatctaca tcattacgta ggtcaacttc ctctttacga
    17101 gctttatctg cttctgcatt tgcttctgca tcttttacca tacgttcgat ttcttcgtct
    17161 gttagacctg aagaagattt gatcgtgatt ttttgttctt tttgtgtacc tagatctttc
    17221 gcagatacat ttacaatccc gtttttatca atatcgaatg ttacttcgat ttgtggaata
    17281 ccacgaggag ctgctggaat atctgtcaat tggaatcttc ctagagtttt gttgtcagca
    17341 gccattgggc gttcaccttg taatacatgg atgtctactg ctggttgatt atctgccgca
    17401 gttgagaata cttgtgattt acttgttgga atcgttgtat tgcggtcaat caatttagtg
    17461 aacactccac ccattgtttc gatacctaat gacaatggtg taacgtcaag taaaacaaca
    17521 tctttcacat caccagtgat cacgccacct tggatagcag cacccatagc aactacttca
    17581 tcagggttaa ctgatttgtt tggttctttt cctgtttctt tacgtactgc ttctacaact
    17641 gcagggatac gagtagatcc accgactaaa ataacttcgt cgatttctga ttgtgataaa
    17701 cctgcatctt ttagcgcttg acgtactggt acttttgtac gttctactaa gtcagctgtt
    17761 aattcatcaa attttgcacg tgtcaatgtc atttccaaat gcaatggtcc agattctcct
    17821 gctgtgataa atggcaagct gatttgtgtg cttgatacgc cagataaatc ttttttcgct
    17881 ttttcagcag cgtctttcaa gcgttgtaaa gccattttgt cttgagacaa gtcaatgctg
    17941 ttttcttttt tgaattctgc taccatgtag tcgatgattt tgttatcaaa gtcgtcccca
    18001 cctagatggt tgtcgccagc tgttgacaat acatcaaaga caccgtctcc tagttcaagg
    18061 atagatacgt caaaagtacc gccaccaagg tcgaatacta agattttttc gtcgcgatct
    18121 gttttatcta aaccataggc aagagcagct gcagttggtt cattgacgat acgttctact
    18181 tctagaccag cgatcttacc agcatctttt gttgcttgac gttgtgcatc attaaagtaa
    18241 gctggcacag tgataactgc tttttcgact ttttcaccta aatagtcttc tgcgaatcct
    18301 ttgatatatt gaaggatcat tgctgagatt tcttgtggtg tatatgattt accttctaca
    18361 tctactttgt aacctgcttc accgatatgg cgtttgattg atgaaatcgt atttgggttt
    18421 gtcacagctt gacgttttgc aacttcccct acttggattt ctccattttt gaatgataca
    18481 acagaaggtg ttgtacggtt tccttctggg ttagcgatga tttttgcttc gtttccttcc
    18541 aatactgcta ctgcagagtt agtcgtacct aagtcaatac cgataatttt actcataatt
    18601 aacgtctcct tctttaattg aaagtgtaag ttttatatcc gcacaattat tgtgcaacaa
    18661 tgaccatggt tggtcttaac acgcgatcat gtaatttgta gccttcttgt agtacttcta
    18721 caatcgtatc ggctggtgta tcttcagtcg ctggaacagt ttgtactgct tgatgaagat
    18781 taggatcaaa ggcttctcct ttagcaggga ttttttcaat tccttcttct tccagtgcgt
    18841 tacgcaaact ttccaagacc atttccacgc cttttttcaa acctgcccct tgatcatctg
    18901 acacttctgt ggctaatgca cgttccaggt tgtcaattga tggaagtaat tttttggcta
    18961 aatcttgtga acggtatttt tgtaattgct cacgctcatt ttttcctcga tttgccatat
    19021 ttgcgatttc agcacgagcc cgcaagtatt tatcttccat ttcttctaac tcagctttta
    19081 atttcgctgt ttcgaattct tccgcttcag cttctgaaat acccgaatct tctgcagctt
    19141 cgacatcgat ttccggttct ggctgagcat ctgtcatttc tttatctagt tcttcttgat
    19201 tttctttcac gatacagtct tcctttcatt attcagtcag ctgtttgatt gctcttacta
    19261 agaaagagag aacgatcagc tgcaaatagc ttcgttattc acattacgag tttgacaagt
    19321 caagcgaacg gtagtaatca gcaagtttag ctgctagttc tcttcgataa acatcgacta
    19381 gaccgaacat ccgagaataa ggcatactag taggccctaa caaggctatt gtacctcttc
    19441 catgaccatc tatctcgtaa cttgcttgaa tcatactcat attctgcaat aaatcattgc
    19501 ctaattcagc accaatcttg atatgaatat tgctatccat cggagtgatc atttgtgtaa
    19561 gtccgtctga gtctttcata aaagaaaaca tcgatttgaa ctgatcaaga tcttgacggg
    19621 gctcataatc caagagattc atttgtccac tgacataaac cttatcttca aaaacatgac
    19681 caagaacggt gtcaaataac tgagacatcc cttctgtcgt ttggaaatat ttatgaagaa
    19741 tcatcggaat ctccgtacga agtttatggt acaccgtcat cagaggatct ccgactagac
    19801 ggtcgtttac aatctgtacc attttttcta gttcctgact tcccatatat tcgggaagag
    19861 tgaacacttg gctttctaca ttccccatat ctgttacgac gatagcaata atctgtcgat
    19921 tattcaaggg tacgatacga aagccagtca gtcggcggtc ttttatttca ggtcccaatg
    19981 aaaaagtcgt atagcttgtc agctcagata atatctcagc agattgctta ataatctcat
    20041 tgatttcatg aaattctctg cctagagact gacgaatcag cagcaaatca tcttctgcta
    20101 cgcgagcagg ttttaacaaa tgatccacgt aataacgata gcctaaagca gatggaatcc
    20161 gcccagaaga agagtgagtc ttcaacagca atccttcatc ttctaatgct ttcatatcat
    20221 tgcgaatcgt tgctgagctg gccttgactc cctcagacat caatgttttg gaaccaacgg
    20281 gcacgccact actcgtatag tgttggatga tcagacgtaa aatgtcgttt tgtcgctttg
    20341 ttaacatctt cctcacctct cattagcact catatattct aagtgctaat ccacttataa
    20401 atatagcaag aagcccttct cctgtcaaga gaaagactcc attttttagc actctaaaca
    20461 caagagtgct aattctaaat aattgatgta aatccagtta tatcaacgtt tcaatagtcc
    20521 tttattgtat ttttgaactt tttttattta aattcagata aaaaacaaat attctttaaa
    20581 ctatttttca aacaaaaagc gctcaaacac attgttcccc aaaaaggtcc ctcttggtgt
    20641 caaataaaca cggtcttctt cattaacaag taacccttct tgctctaatt cccttagcac
    20701 atcaccgtac accgattcaa ttgttttttg atagcgatca tagaaatgtt ttttagtaat
    20761 ccctgttttt tttcttagtc ctaagaacat ttcttcttct atttgatttt ttcgtgtcaa
    20821 tttttcttct tccaataccg ggcgttcatt tgctcgtaat gggcgtaaat agtgttggat
    20881 tggtcctttg ttgcggtaac gaatatctcc aagataaccg ctagcaccag ctccaaaccc
    20941 aaaatagtgg gcattgttcc agtacatgag gttatgttga gattcatgtc cggagagccc
    21001 aaagttactt atttcatatt ggtgacgtcc atgcttttcc attgcttcaa tcgtctcttc
    21061 aaacattctg gtttccgttt cctgatcagg cagttcgagt cttccttgtc gtacccagtt
    21121 catgaacatt gttttgtttt cgaggatcaa tgagtacaaa gaatagtgag gcagatccaa
    21181 agccaaagct ttttccaaag tatccagata tccttctagt gtttgtcccg gtaatgcata
    21241 aatcaaatcg atgcttacat tggtaaaatt ttctgcttct aaaaattgca tagtttgata
    21301 cacatcttct gcggtgtgtt tacgtccaat ttttttcaat aagcgattat caaatgtctg
    21361 cactcccatg gataaacgat tgacgccata gtttttcatt acttgaagtt tttcacgggt
    21421 caaatcacct ggattcgctt ctacagtaaa ctcattacca ttttgaaaag gcaggatctc
    21481 tctagcacct gataacagtc gatcaagttg tttagcagat aaagaagttg gtgtcccgcc
    21541 ccctacgtaa agtgtttcta aattcgctgt cggatgactt tctagcatta ttttcatttc
    21601 attcagcagc atttccacat actcatctac tggctgccct tctaaaaaaa ctttattaaa
    21661 atcacagtaa tagcagatat gctcgcaaaa tgggatatga atgtacgcag aaaccttgtt
    21721 ttctgcggga tttattgttt ctatcattga aattctttct ccttacgtcg aaaagtcgtg
    21781 cctgttttcg accttcttaa aatcgcgttc aagcaccaac tcattgaaat aagccgcata
    21841 tttagaatca aggaaaccta gctctaaaaa tgcttgctta ttgtcatcgg agctacacgg
    21901 tgcttttaca gcctctcgat aaacggtaat cgcaaagctg accttcggca ttaagtcaaa
    21961 atctgcaaaa atatgaaaag ctattttcac agattcctcc ttaagtgctc agaccacaac
    22021 gctttgctta caacctctca tatacgcata ctaaacgtat ttggcgaaca aatccactaa
    22081 aaaatccaag aatcgacttc ttggatgatt ttttctttca ttaaactggt tacctagtta
    22141 tatatctcta gggttgtttt catgtctgag tgcccgatat gatttatttt tcgtttctat
    22201 aattactttt tgccttccag tttcttttaa tattgtactc aagcaagtgg gaattggttc
    22261 ttttgtcatc catgtatgca aaatgttata atgctggtaa acaaaaatga ttacctgtca
    22321 tttttattta gttcgtattt aattaagttg actatctgta tactctgtct aaaacttaag
    22381 aattgttgtt ttcgctgtcg ctacccaatt ttaatgaatg tctgtaggtt aaaactgttg
    22441 tccttgttat agatagtaaa ggagagcttg aaaatgaaaa ctgcgattac gtttctatta
    22501 gaggaatatg ccgattggga aggtgcttat attactagca cactaaacca aaatgagaaa
    22561 tggcaaaaca aaactgcttc tatccaatca gaggtcactt ccattggagg attaaaaaca
    22621 aaaatcgatt acttaatcga acaaatacct gaaaaagttg acctttttat tatgatcggt
    22681 ggcaattcat gggacataaa aaatcatcat ttatatcaaa taatcgataa attgttaaaa
    22741 acagacgtac cagtagctgc tatttgtggg gcagttgatt atttagctca gagcggatta
    22801 ttgcaaggtt acaaacacac cggaaatgcg caatttcctt ggttagaatc ggaaaattat
    22861 cagaataaag aagattttat agaacaacaa gctgtgaaag atcgtaattt gattactgcg
    22921 aatggaacag ctcctataga attcacagac ttgattcttc aagcagtcca ttttgaaaca
    22981 gaagaaataa gagaaaaaac gattcgtcta tacaaacttg gattctatga ttactataaa
    23041 aagtacggag atcctttctc ctaatcattc tcttcaatta aatactcctt atcaatagtg
    23101 tgacaagtaa ttttactgat gattatttgt cacacttttt ctgacattta tcaaagaaga
    23161 tacactctat ttatattcat agacaaggtt ttacggattc aaaaattcag gattatgcta
    23221 tactggtatt aaaaaaataa tgaggtattt tatatgaaaa agttaggttt tattttattg
    23281 ttggtctctt tattccttgt aggatgtacg tctcctagta aagaaaatag tgatccatct
    23341 gcgaccacag acaaaagttc aatcagacaa accagttcag ctgaaaaatc aagtactcag
    23401 aaatcagctt ctactgaaac gaatacatct tcaacaagca gtaaagtctc agagcccaat
    23461 acttcttcta catctagtag tacagttgag acaaatattt cttcttcaac aatagacaca
    23521 acctcctcac agtcttctca gtcaatagat ccgttagcag gatattctga tctacaaatt
    23581 gagtacgcac gtgtttggct agctattaga ggtaatactt ttagagatac atcgggtgat
    23641 ttcgaattac atgttattca ttatcctgca ggaacaccta tcgcccctta tgatgcaggg
    23701 agtgcagtct atccgaaaga tgtggttatg ctcactggga aatacagtta ccagggagtt
    23761 ttagtgtatt caagtaacca tgacgggaca atcactagtt atccagttcc ttcacattgg
    23821 caaatgcctg cagatcaaac atcttatcca gcgttcatta gagaaacaac tcaagaaatc
    23881 attgataatg catctgtagt agctattcct acaggaaatc ccaatgacgt gaaacagtta
    23941 atcgatgtga cagttataga taattagact ttctaatatg ttatacaaaa agaagctgac
    24001 agcattttgt cagcttcttt cactttcttt tctatttaga tttattggtc tttgatagac
    24061 tccaaggcag gaaaaaattt tttaaataaa cgtttgcaaa acagtcgctt ttctatcttc
    24121 taccgctact tctgcatagg caccattaat caatgtcagt tgtggcggta catgaccaca
    24181 atcaatatca tacaagatcg gtagttttaa ttcctttgac agatcttcgt atacatcctt
    24241 cattttataa tctaacaaat cctctgccgc acttctaccg aacatgatcc ccgagctgcc
    24301 atcaaaccag cctgccagtt tcatctgaac aagtgatctt cttaaatcaa ctgacgatag
    24361 ctcacagttt tctagaaacc acaagatcgg ctttccatca ttatactttc ttttaaaaga
    24421 ttggacatct ccataaggag taccgattaa atgcctgatc gtatcaatac aaccacctaa
    24481 taatcgtccc ttcatcctta ctgcatctcc gctgactgat tgccaatttg ttggttcagt
    24541 gagatggaaa acagaagatg atgaatgctc atggggccac actttttgat acttttcaga
    24601 agattgctgt agaatcgatt ctccttctct tgttttcagt acactttccc acattgctgt
    24661 cgtttcatcc aagtactctc ctcttaaatc aataaggtta gttccatgtg cagtggctat
    24721 ccctgttttt aatgtgacag caagtagcaa cgtactaata tctgaataac ctactatcca
    24781 ttttggttgt agagcatcaa aatcgacaaa atcgagaatc tcaatcaata attctcctcc
    24841 ccaagtcgga aagatcattt ggatattttc atcttgcatc atttgattga attcatctgc
    24901 tcgttttctt gctggacctg atttttcctt atcttgcgtc caaagcgtct ttccagtaac
    24961 tacttggaaa gatttttctt ccattctctt taaagctgat tcgagtaacg aatggtgttc
    25021 ctctgatacg cctgaggatg gtgcagtaac cccaatcgtt gcatttcttt ctaataatgg
    25081 ataacttatc atgttgcttc acctccaagt atgcactatt ttacttggtt aattgttttt
    25141 ttcgtttgcc ggcatcaaaa atatcggcat ctcaagtagc tggatgattt tgtttaagtc
    25201 aaccgtttgg atgaccatcg tttgatctgg tctacctggt gcaaaaacaa gtttttcatt
    25261 tgataagagc tcttcatcaa taataatcgg aatctgtttt tcataaccaa acggagaaac
    25321 agcgccctgc ttttgtcccg tttgttcttc catgcgttct gatgaaacaa tagaaagcct
    25381 tttaccgacg atcttcccta atgctttgaa atccgtttgt ttcgtagtaa aagtcagata
    25441 tacgtagtta ttatcttttt tatcttttaa gaataagctt ttggtctctg tacctgtaaa
    25501 tccttgttct ttctttatta ccaaagcatc ttcatttgta taaatggctc gatgatgata
    25561 caactcaaaa tatatatttt cgtgtaagaa tttctttgcc aagtcaatca tttttatgtc
    25621 tcttttctat tttattattt gagacatctt atcatataaa tatagcggtt acaacattac
    25681 ttgtgttttg ttcgtttttt atacataact attgtttttc tggtaaaata aatcaaacca
    25741 cttaatggag gcgttttaga atggaaaaat ttcagccgat catcaatcaa atcaaagaaa
    25801 aagaacatca acaaaagatc aaagaccttt ttgctaagat aacagaaacg tatccccagc
    25861 tagacacaac tgtaaaatgg aaccaaccta tgtttactga ccatggtacc tttattattg
    25921 catttagttt tgctaaaaat catttttcta tcgcaccgga aaaagcagcg attcgtgccc
    25981 ttgaaaaaaa tatccaagaa gcaggctacg tatatacgga taacgtcatc aaagttcctt
    26041 ggaaaagtgt aatcaactgg gaattgattg aacaattgat ttcatttaat attgaagata
    26101 aaaaaggtca tgataaattt tggcgataaa gctttatacg aataaacctt ttactggcga
    26161 ggaaactatt catcacctta tacaggtttt gttcaatcct atccttttta tcatccctgg
    26221 ttaaacactt aattgacaaa gtatctgcta tttttataag gagtctttct tgtgctgaaa
    26281 aaaatcactg attgaatggc tttgaatttt ctacttagta aaattatttt ttcagcaatt
    26341 ttcctgcaaa acttttgaat aaggaaaaaa tacgagaaaa tatcagtgat ctttcataat
    26401 actgcccatt attttcaggt atatttaaat ataatatcac tttaggagag aacgaatttc
    26461 tttagagaaa taaaagttag aattttctga cttgattttt acaatgacct taattaaaat
    26521 cgttctttct acatttcgtg attaagcaaa tatcaactaa gtcagtagaa ccattcacat
    26581 aggagggtga acattgaaag atttttactc agtaaatgaa ttagccgaac aattaggtgt
    26641 tactacacgt tctatcagaa attatcttca tgaaggaaaa ctaaaaggaa caaaggttgg
    26701 cgggcaatgg aaattttctg aacgtaatct ttttgagttc ctatacggag atcaagcaga
    26761 ggaagcagca aaagaaatgc agcgattcat gctaaatgca ccaatcacga tgcgtttcaa
    26821 ccttcaatat cgtgatttta cagcaatcaa tcaattccga gaacaattag tccagtacca
    26881 taacgatgtt tacgcaaata aaaaagaccg tcttttacaa tacgatcttt ataaagacaa
    26941 tcatgcggag atactaatcg gtggtaactt caattatgtc gtaaacttta gtcaatggat
    27001 caatgaaaaa ttattgatcc agacagatat ttcccttgta tcttaaacaa ttaaaaagcg
    27061 ctagaaattc cagagatgtt ggaaattcta gcgctatttt tttataagta cttactgttt
    27121 ggttcaaaca aatatttttt taataaatga aattttacca tctttatcct caaataaaga
    27181 ccgtgaccat gcttcctact acgatcagca ataatccgat aatcgttaat tttttttctt
    27241 ttggtgtctt attttcatgc aggatgtaca taccgcctaa tgtagagata actacgttta
    27301 gctgtgtaat gatatatgca gtagcaaccc cattttcatt tgctgaaaaa atataagcca
    27361 atgctgacac actaaagatg gctccaacga tcgttgcttt ccaactcttg tcttcagaaa
    27421 atgcggttgg tttacggaaa atcacataaa tcactgcccc taaaaagaca ccgatcattt
    27481 gcggaaagaa aatggaaagt ccgcttgcat caactagttt aggcaatgca ctgtataccc
    27541 aataaccaat acttgttgat gctaatagac taattccttt tgtgataccg cctttttttc
    27601 caggatcatc actgattgcc gttaaagcag aaccaaatac aagaagaatg attgctgctg
    27661 ctccaatgat tttccccata cttccagacc attctccaaa agcgaagaca ccaatcaacg
    27721 aggttccgac taactgtaat gcagtcgaaa ttggcatcgt tttagaaaca ccaatcaagt
    27781 tgaaagatgt atactgtcct gtttgtccaa taacccaaaa tgcaccagaa agtaaactaa
    27841 tcaaaaatgt agtcactgaa ataccagctg gagaagcgaa taattgaacg agtaagccga
    27901 caagcaccgc tccaatacca gttcctaata tctcatttgt tggtctgcca ccaatctttt
    27961 tcaaaatcag tggctggatc ccccagccaa ctgccgggat caatgcaatc aataaaccca
    28021 tttttattca cccttatcct tttccacctt ggaaagaagg atataaagtc atcccaccat
    28081 ctgcaaatag tgtaatacct gtaacataac tggattcgtc tgaagccaac catgcagctg
    28141 tcgccgctat ttcttttggt tcacctacgc gtcctagtgg gatcatactt tccaagagtt
    28201 ctttttgttc aggatcatca aattttgccg cattaatagg tgtacggata gcaccagggc
    28261 ctatactatt gattctgatt cccttcggtg catactccat tgctaccgtt tcggtaaata
    28321 attttacccc gccttttgaa gcagcgtaat gagcaaacgt tggccatggg atctgttcat
    28381 gaacagaaga catattgatg atatttcctt ttttattatt ctctgtaaag taatttaaag
    28441 ccgctttcgt tccgataaaa acgccagtca aattgacttt taacactctt tcccaatctt
    28501 caaggctaag ttcatgggtt ggatgctgat tttccatccc agcattatta acccaaacgt
    28561 ccagtgtccc aaaattttct aaagccgtat caagtaaacg ctgtattcct tcttcagaac
    28621 tgacatctgc ttgtacagag actgcttttc cgcctgcctc ttcgattgct ttgactgctt
    28681 tttctgctcc ttctttatcc gagttatagt tgacaactac attcattttt tctttcccga
    28741 atcgttgagc aatcgctgtt ccaatcccct ttgagccacc agttactact gcgactttgt
    28801 tttctaattc cttgtacata gtacccactc ctttttcata cctaaacctg ttattacgga
    28861 taacttttat ttccgcctta aagtgagtgt acaccaagat atatagtcta aagcaaatga
    28921 tacgctctag caatcgtttt catgaattgc tagtgtcctt ttatttctat aaagaaagag
    28981 gttgtgacag aagtggtcag cttcaagaaa tttcggctta tttccgaaga agctacttct
    29041 gaaaaaccat ttatttggaa aaaaaggctg tgacaagact tttgtcacag cccctttcct
    29101 gggcttattt atcacccatt ttattgtaat tgctagttat acataaacaa gagattttat
    29161 gcataatgtt ttattgctga gaaaaatatt cagctgttgc ttgttcatcc tccttgagtt
    29221 gttcaatcaa ttcctctaca ctagaaaatt tcacttgatc tcttaaaaat tgattccagc
    29281 gtaccgcgac atgttcacca tagatatctt gattaaagtc caaaatatac aactctacag
    29341 tcagctcccg tccttcaccg aatgtatcat tatgtccaat tgaacccatg gctggatacc
    29401 atgtttcccc aatttttatt tccgacacat aaacaccttc tttaggcaaa tgaaccgtgc
    29461 tctttacttt gacatttgct gtagggaatc ctagtagtct gcctcgtgca tcgccatgga
    29521 caactacccc ttctacctca tatgtgcaac ctaatagttt tgctgcttct tttacttgtc
    29581 cgctgtctag taatccacga atacgggtag agctgatttt ttcaccttgt tcttcttctt
    29641 tagaaactgt gacgattttg aatcttcctt tggcatattc aggaaaatga gcgacatctg
    29701 caatgtcttt tggtccatac gtataatcaa aaccggaaac agcatatttt gcatgtaacc
    29761 ctacgatgta ttgatcaaca aattcctgcg gtgctaaatg agcaaaagaa gaagtaaaat
    29821 caacttcata taaataatct atacccaaag cggccatttt ttcttctttt tgcttaaggc
    29881 tagtcaaata tttcacgctg ctaggatcga tctttttaaa gacaatagat ggatgctgat
    29941 tgaaagtcat caatgctaat ttcaatcctt ctttttgtgc aatttccata cctgtcttta
    30001 tgactttctg gtgtccgata tgcacgccgt cgaaaaatcc cagcactaaa acgacatcgt
    30061 cttctggaat ttgttctgat tggtaaggat gtctgatctt gatgatattc attcgttatt
    30121 cctcatttct caatacttta ctaggtttta atttgttttt ttctttcggg tttggctgat
    30181 aaatactgac aacttttcct ttataaaaca aagcaatttc ttctgacggc atttctggga
    30241 gcgcaaatac agtataatca agtctcatac cgttttttac tttctgccat acttcatcag
    30301 taatatctac tcgtttgaac cgttccactc ctttttctat tggcagcaaa caatcatcga
    30361 ttgtcccatt tttcacagct tgagctactt cttctaatgt cagagcatcc ttctgggaca
    30421 taccggcact tgctgttcgg gtcagatcag acatgtgcgc agggtaacca agtttcttgc
    30481 ctgtatctac agctaatgtg cggacatagg ttcctttccc gcattctact tcgaagcgcc
    30541 aagaaatggt tcctgtcact tcgtctcttg tcaattcact tgtccggata aaacggtaaa
    30601 tctgagcctt tctaacagga cgctccactg tttcattatt gcgggcatat tcatataacc
    30661 gcttaccgtt gacttttacc gctgagtaca ttggtggtat ctgggtgatc tcaccaataa
    30721 atgaagccat cacttcatct attttttttt cagataaaga catatctact ggttttgttt
    30781 ctacccgttc tccggatcga tcttccgtcg ttgtactgaa acctagagtt atctcgccta
    30841 catagatttt cccagaatct gtcaaatatt cgataacttt tgtggctttt ccaatacaaa
    30901 tgggcaacac accgtctacg tctgggtcaa gcgtaccccc atgtccgatt tttttcgtat
    30961 gtaagatttt tcttaatttg aatacgcaat catgacttgt cattccgcgt tctttccata
    31021 aaggtaataa cccgtccatg tcttttctcc atttcatttt tgatttaact gaattattat
    31081 aacacattgc atcaattact gacgaatttg acatcataat gaacggtttt tatttttcgg
    31141 caataaaaac gtaacaaaat tcatgctcac gtaaatagaa atcatgttat gcttttttaa
    31201 aggagggaat agaatggaag attttttctt tgcaccattt gattggattt atgggtggct
    31261 tacaggtaaa tagtttggtt ttcacttccc aaaaacgaaa aaaaagactg gccattcttc
    31321 cagtcttttt tccgtttttt ttatttattt tttcagtttc ttaatcaatg taaaaaattc
    31381 tattttgcgg gatcgatttt atcattttat tggtcaagat ttttccattt ccaatccaat
    31441 acttctggca gatcaatacc attttcacga atatagtttt gatgttcttg taatttctga
    31501 tccatttttt tcgcaaaatc ttctgcttga cttcctttga tttttaaagc tgcgtctttt
    31561 gccaaatgat aacgatcaag ttcatttact acacgcatgt caaatggtgt cgtgatatct
    31621 ccattttctt tgtagccatg aatcattaaa tgatggttat gacgatcaaa gaaaatcgtg
    31681 cggaccaatg tttcataacc gtggaaagca aataagatcg gtttgtcttt tgtaaaatat
    31741 tgatcaaatt cttcatcgct caacccacgc ggatcttgac ttggatgacg taatttcaat
    31801 aaatctacga cattgatgaa ccgaattttt agttttggga attcttccag cagcaaagtt
    31861 acagctgcta atgcttctaa gttagattcc gttcctgcag cagcaatgac caattcaggt
    31921 tcctcctctt ttgctgtcga agcccaatca atgattttca agccttcatt cactaagacg
    31981 gctgcttctt ctgctgagta aaattgagca cgcggatgtt tggaagagat gatcaaattg
    32041 attttttctg acgagcggaa tgctttatcc atgactgcga gcaatgtatt tgcatccgca
    32101 ggcagatatt cacggatgaa ctccgccttc ttttctgcca aatgtgtcaa tattcctgga
    32161 tcttggtggc tatatccatt gtgatcttgc tggaagactg ttgagcttgc aatgagattt
    32221 agcgaaggat aatcatttcg ccaagaaagg tcatgtgatt tccgcatcca tttgaagtgc
    32281 tgagtcaaca tagagtcaac cactcgcaag aatgattcgt aacttgcaaa aaaccatgcc
    32341 gtccagtcag aacgtaccct tctaagaacc cttcatcttg gtgttccgat aattgactgt
    32401 cgatgacgcg accagatggc gaaagccatt catcattttc tggttcgatt ttttccatcc
    32461 attggcgatt tgtcgttttg aaaacttgat tcaatcggtt tgattttgtt tcatctggtc
    32521 caaatatacg gaaattttct ggatttttct caatgatctc gcgaacgaat ttaccaagtt
    32581 cggtcatatc ttctgcttta acggagcctg gtttttcaaa ttgcaaagta tattttttcc
    32641 aatctggcat aataagcggt cttggatcga ttccaccatt ggtgatagga ttttttgcca
    32701 ttctttggtc accagtaggc gcgatttctc tgatttcttc taaaatacgt ccttgtgcat
    32761 caaataattt ttctggttca taggatttca gccaacggag taaagcatct gcatgatcca
    32821 tatgttcttg atctacagga atcggtactt ggtgtgctcg gaaggttcct tcaattggtt
    32881 ccccgtccca ttcttttgga ccggtccatc cttttggtgt gcgtacgatc aatactggcc
    32941 attttggcat cacgacatct gttgctgtct tcaggcgagc ttctttttgg atggatttaa
    33001 ttttctcgat tgcttgatcc attttttcag ccataacagg atttaatttt tctgaatcat
    33061 ttccttcaat aaagaaaggt tcccagccaa gtccgttaaa gtaatttgct aattctccat
    33121 ctgatttacg tgccaagatc gttgggttgg ctatttttgc accatttaaa tgaaggatcg
    33181 gtaaaacagc tccatctatc actggattga taaagacatt tgagaaccag cttccagcta
    33241 aaggaccggt ttctgcttca ccatcaccaa tcacgacagc agagattact tcaggattgt
    33301 ctaatacagc tccgactgca tgtgaaagag agtatccaag ctctccgcct tcatggatag
    33361 aacctggtgt ttctggtgct gcatgagaag caatgcctcc aggaaatgaa aatcgtttga
    33421 ataatttttg cattcctgtc tcatcttccg ttacttctgg atagatctct gtgtaacttc
    33481 catctaaata agcattcgat accatgactt gtccgccgtg tcctggccct tcaatataaa
    33541 acatgttcaa atcatattta ttgatgactc ggttcaaatg gacataaata aaattttggc
    33601 caggaatcgt tccccaatga ccgattggat gttttttgac atcttcgggt ttcagtgtac
    33661 gtctcagtaa ggggttatct ttaagataaa gttgcccaac agaaagataa ttagcagcac
    33721 gccaccaagc cgtcatttta tcaaagtatt ctttactaga ataatccata cataacactc
    33781 cttttcatta ttactcttat tctagcgcag atactcccaa ttgaaaccta ataagcactt
    33841 ctgttcattt gtttggattt tatatctagc caaatgaaca ggtataaaaa accacgaaat
    33901 ttttcctttc gctgacgttt tttacattga tccaaaaaac ttgtcgttat cacaatggct
    33961 gttccgcagt tcatttcgct cctcatcatg cataatttgc tgaatgacgc tgggccagtt
    34021 aacacattgc tggaaaactg gggctggatc gagcaattcc tttcctgatt gatccgtttt
    34081 atgtaaaagt ctctgttatc tttgtcaata tgtgaattgg tatcccagct actatgctaa
    34141 tttctacagg gattatccaa aatttggatc gtgaccaaat cgaagcggcg taatcggaaa
    34201 tatcaataac ttcaacgtga tttttctttt aactgtcaac ggaccgacaa actcaaactt
    34261 ttatggtgca ggttccactg atcttctagt tacttggcta tacctgactg tcactaaaat
    34321 agattacaat ctagcttccg ttatcggtat cttgatcttt atctatatgc aaaaatttat
    34381 atactactag aaaaaagaaa gtcgaatttt ttacttttct ttttctcttt tttggttagc
    34441 tgacacgccg caagactaaa gtcatggaac tcttaggtaa tacgtcttaa tgaaaacaaa
    34501 aaaatcccgc aacccctgat ttcacaggat ttgcgggagt ttttgattag tctttgttca
    34561 agtcgcgaag caattcatcg atatggttgc catagacaac tgattcatca agttcaaaga
    34621 acaattcagg tgtcttataa atacttagat tgtgccctaa ctctcgacga atcaatcctg
    34681 atgctttatt caatccatct tgtgctgttt tcttatctga tgctaagtca gataaaatgc
    34741 tgtaatagat cgttgcttgc tgtaagtccc ccgtaacttt cacatctgtg atcgttacgt
    34801 tctccacacg agggtcacgg acctttttgc gcaaaatatc attgacttct tttaagattt
    34861 cttgaccgac tcggcggtca cggtagttag ccataatgat tggcctcctt ttatcagttt
    34921 ttagtcattt ttgacttctt ccatgataaa tccttcaatt acgtcatcta ctttgatgtc
    34981 attgaatttt tcaatcattg caccacattc ataacccatt ttgacttctt tcacgtcatc
    35041 tttgaatcgt ttcagacttg caagttggcc ttcatagatt acgattccgt cacggattac
    35101 acggacaccg ctatctctac gaatatatcc ttctgttaca taggcacctg caatcgtacc
    35161 aactttagaa actttgaagg tttcacgaac gatcatttga ccggtgattt tttcttcaaa
    35221 ttcagggtct agcatccctt tcatcgcagt ttcgatttct tcgatggctt tatagatgat
    35281 acggtgaaga cgaatatcga cttcttcagt atcagcttgc acttttgcct gcggtgtagg
    35341 acgaacgttg aaaccgatga taatagcgtt gctggctgct gctaaggtta catcactttc
    35401 attgattgct ccgacagctg aatggacgat ttttacacgg acaccttcca catcaatctt
    35461 cttcaatgat gcagccagtg cttctgctga accttgtaca tctgctttga tgattacgtt
    35521 aacttctttc aattcgcctt ctttcaggct ttcaaataag ttatccaaag tcacacggct
    35581 tgtggcagca cggttttcta ataatgcacg tttcgcgcgt tcttcacccg ctgcacgtgc
    35641 tgtcttttcg tcttcgaaga ctacgaaacg gtcacctgct tgaggtacat cattcaagcc
    35701 agtgatttct actggcgttg caggtcctgc ttctttatcg cgacggccaa gatcgtttgt
    35761 cattacacgg acacgtccgt aagtattacc gacaacgatt gggtcaccaa catgaagtgt
    35821 tccttcttgg acaagtaagg ttgacacagg acctttacct ttatcaagac gtgcttcgat
    35881 cactgttccg attgcgcgtt gtgttggatc tgcttttaaa tcttctactt ctgcgactaa
    35941 caggatcatt tccagcaatt cttcgatgtt ttggccgaat ttcgctgaga tctcaacaaa
    36001 gatcgtttct ccaccccatg cttcaggaat taactcatac tcacttaatt cttgcataac
    36061 atgttgaggg tttgcacctg gtttatcaat tttatttaca gcgacgatga ttggaacgcc
    36121 tgcagctttg gcatggttga ttgcttcaac tgtttgtggc atgacgccat catctgcagc
    36181 aacaactaag atcgtgatat ctgtgatact tgctccacgt gcacgcatac ttgtaaaggc
    36241 cgcatgtcct ggtgtatcca agaaagtgat tggtttacca tcaatatcga tctggtaggc
    36301 accgatatgc tgtgtgattc cgccggcttc gccgcttgtt acacgtgaat ggcgtaatgt
    36361 atccaataaa gttgttttcc catggtctac gtgtcccata atcgttacga ctggcggacg
    36421 tgagactaat ttatctggat cgacttcttc tgcctcgaag aatttatcga tatcagcaat
    36481 gtcaacttgg actttttctt gtggttccat gccatagtcc gtcgctaaca gttcaatcgt
    36541 atctttatcc agtgcttggt tttgattgac catgacacca agcataaata attttttaat
    36601 gatttctgct ggctcacgat gaattttttt cgcaatatct gctacgttca taccttcagt
    36661 atactctaat acatctggca attcacggaa tttgcgtggt ggtacagctg gtttagttga
    36721 ttgttgctgt ttgccttttt tacctttttt attataacga ttacggttat tattattaaa
    36781 tttgccgcga ttgttattgt ttggtttgtt gttattgtta gtacggtttt gattgccgcc
    36841 ttggctcgtt ccttgacgat tttggctgct ggtatttccc gtattatttt ggtttgttgt
    36901 tggttttttt ccttgattca cttgaccgct cccttgaccg cgatcttgat aactgcggtt
    36961 atttttattt ttttgttgat tgttcgttgt ttctttttta cgttgatcgt tcggctgttg
    37021 attggctggt tttctgcttg cttgttgtgc agcttgttgt ttcgtgcttg ggtttttgaa
    37081 cgcttgttgc aatttcttct catcattgct agtaatcgca cccatatggt ttttcacatc
    37141 gatgccaagt tgttgtgctt tttccacgac gtctttacta gattttttga gttcttttgc
    37201 taattcatag attcttttgt tgcccatgca atcaccttcc taaccttcaa ttagctcctt
    37261 ggtcttcttc gcaaatccgg catccattat tccgaatacc ttgcgaggtt ttccgatcat
    37321 ctgcgtgatt tctgcttcag aaaatagctc aaagcaagga acttcataat atgaactctt
    37381 atcttttatc ttttttcttg tattctcgct tgcatcggtt gccacaaaca ccaattttgc
    37441 tttttgtttg cggatttcag caatggtcag ttcttcaccc gtgattagtt ttcccgcgcg
    37501 catcgctaga ccgatgagat tcatggcttt ttgccgatta actgtattca ttttccaaat
    37561 aactctttac gtgctttttg gtgagtgaca tagtcaagaa gttcttgata aaactcatct
    37621 gttaatgttg tttcgagtac tcgatcgaga atttttttat cccaagcttg ttgtacttct
    37681 tccggctcaa gtgagacata cgctcctcgt ccaggcattt ttccagttgg atcgatcgat
    37741 acttcccctt cttttgaacg ggtgattcgg accatctctt ttttaggttt catttcacct
    37801 gaaaccacgg atttgcgtaa aggaattttc ctttgtttca tctctttgcc tccttactaa
    37861 agttaagctt ctggtttttc ttcgaccgtt tcattgttga atgcaattgt ttcatattca
    37921 tcgtctgtta aatctgactg aacgattgct tcatcatgca gttcttctgt ttctttttgt
    37981 tgttcttgat tttcataaaa ttccgtcatg tcagattctg atttgatatc gattttatgg
    38041 ttagtcaatt tagcagctaa acgtgcgttt tgtccgcgtt taccgattgc tagcgataat
    38101 tggtagtctg gaacgacaac tgtgcaggcc tttggatttg tctcatcgaa gatcacatcc
    38161 atgacttgtg caggattcaa tgcatttgca atgaatacag ctggatcttc attccattcg
    38221 acgatatcca tgttttcgcc ttttaattca ttgacgattg cttgaactcg ttgtcccttt
    38281 ggcccgacac atgtaccgac tgcatcgata tttggatcat ttgagcgaac tgccactttt
    38341 gaacgatcgc cagcttcacg ggcgatgctg acaatttcaa cgattccatc atagacttct
    38401 ggtacttctt gctcaaataa gcggcgcaat aggtctggat ggctgcggct aacaaatact
    38461 tgcggacctt tagacgtatt ttctactcgg gaaacataaa ctttgattcg atcatgtggt
    38521 tgataaaatt catttggcat ttgatcttgt ttagaaagaa ctgcctcaat tttaccaaga
    38581 ttgacataaa tataacgttt atcctggcgt tcgacgattc cttgcatgat gtctttttca
    38641 taagcactga attcgttata aataatcgtt ctttctgctt cacgtacacg ctgtaaaata
    38701 acttgtttag ccgtttgcgc agcgatccgc ccaaaatctt ttggtgttac ttcaaaacgg
    38761 atcgtatcac cgatctcata tgctggattg attaaaagtg catctttcaa tgagacttcc
    38821 aactgtgaat ccatcacttc ttccgttact tcttttaccg catagacatg gatctttcct
    38881 tttttctgat caaattccac ttcgacgttt tgtgcttggc catagtgacg tttgtatgca
    38941 gaaaccaatg ctgcttctaa agcatcgatc acgatttctt ttgagatccc tttttcagct
    39001 tctaaagcgt ctaacgcatt taacatttct ttactcattt tttctgattc ctctccgtga
    39061 ttaaaattgg atagctaagc gagcttttgc aatatttttt cggtcaaaag tcaattcttt
    39121 ttctcgtgtt tttatgcgta ttttcaatgt aagctgttca gcatcaaatg attgaagaaa
    39181 tccttcatat tgtttttcgc catctactgg ttggtataag gagacatgga cataatcacc
    39241 aagtgccttt tcatagtcgc tttctttttt caatggccgc tctgctcccg gggaggatac
    39301 ctccaggaaa taagcttgag gaatcggatc tggatctgct gcatcaagtt tttcactcag
    39361 tttctcactg acaaaggcgc attcttcgat atcgataccg ccttctttgt caataaatac
    39421 tcgtaagaac cagctttttc cttcttttac aaattcgact tcgacaagtt caaaattttg
    39481 ttcatctaaa atcggtgtca ccatttctgt gactgtttca acgacgctac tcaaaagttt
    39541 cgcctcctta aaaaattaag tgcgtaattc cattaaaaag agtgagcgaa aaatccgctc
    39601 actcacagtt agtatcatta cctataagaa ctataccata ctctgcttaa aattacaagc
    39661 agacaggaaa acagatgctt atagcatatc gaataacgat aattgatttt catctggtag
    39721 atctttcaat acgccattgt cgttcatata ttcgatcaat gtttttgaaa ctttaccgcg
    39781 ggtggcaaga tcttctttgg ataaaaatgg accgtctttt cttgcttcaa cgatttgttt
    39841 tgccacgttg gttcccaaac ttgggacagc tctaaatggt gcgatcaacg tatcaccttc
    39901 aataacaaag ttaacggcat ccgatttata caaatcaatc attccaaatt tgaatcctcg
    39961 ttccagcatt tcattggcta actccagcac tgtcagttga ttcttttctt ttacggaagc
    40021 atctaatcct ttgtccgtga tttctttcat tgcttgtttc acagcatctt ttcctttgca
    40081 catggagact agatcaaagt cgtctgcacg tacagaaaag tatgcacagt aatacaaaat
    40141 agggaaataa accttgaaat aagcgacacg aagagccatc aatacgtaag ctgcagcatg
    40201 ggctttcgga aacatatatt tgatttttga acaagaatcg atataccagt cgggcacatt
    40261 attttctttc atcgccgata gataggtttc tcgcaattca tcagggatct tgttccattg
    40321 tcctttacga acagtttcca tgattttaaa tgccatcccg ctatctaatc cagcatggat
    40381 caaataaacc ataatatcat cacgacagcc gattacttcc gccagtgtcg catctcctcg
    40441 tcgaatcaat tcttccgcat ttcctaacca tacgtctgta ccatgagata gtccggaaat
    40501 ttgcagtagt tctgcaaaag ttgtaggatg cgtttcttct agcatccctc gaacaaaacg
    40561 agtgccgaat tctggaatac ctaatgtccc tgttttggaa taaatttgtt cctgacttac
    40621 tccaagtact tcaggaccgg caaatatccg catcacttcc gggtcatccg tagggatcgt
    40681 ttgtggatcg atccctgata aatcttgaag catccgaata acagttgggt catcatgccc
    40741 tagaatatct aatttcaata cattatcgtg aatcgagtga aaatcaaaat gagtcgtttt
    40801 ccattccgaa ttttgatcgt ctgctggata ttggatcggc gtaaaatcat agacatccat
    40861 ataatcggga ataacgatga tcccccctgg atgctggccg gttgtccgtt tgactccagt
    40921 agcgcctttt gctaatctgt cgacttctgc acttcgaaac tgcagattat tgtccctttc
    40981 gtatcctttt acatagccat atgctgtttt gtcggcgact gtaccaatcg ttccggcacg
    41041 gtaaacatag tcttcgccaa acaatacttt cgtatagtta tgggcttctg cttgataatc
    41101 acctgagaag ttcaaatcaa tatcgggcac tttgtctcca tggaatccta aaaatgtctc
    41161 aaatggaata tcatgaccgt ctttgtttaa gcgtgcacca cattttggac attgtttttc
    41221 tggcatgtca aatccagaac catacgtacc atcttcaaaa aactcggaat attggcactc
    41281 tggacaataa taatgagggg ctaacggatt aacttcagtg attcctgtca tcgtcgcaac
    41341 aaagctagag ccaacagagc cacgtgaacc tactagatag ccatcttcat tacttttatg
    41401 cactagtttt tgggcgatca gataaataac cgagaagcca ttaccattaa tagaattcaa
    41461 ctcttttttt aatcgttttt gaatgatctc aggtaacgga tctccgtaca tttgttttgc
    41521 ttttgtgtaa ctcaattctg atatttcatc ttctgaacct ggaatcttcg gtgtataaag
    41581 ttcatctttg actggaatca cttcttcgca gatatctgcg attttattcg tattttctac
    41641 tacgatctct ttcgctgttt cttcccctaa aaagtgaaat gcagttagca tctcatcggt
    41701 tgttcggaaa tggacatccg gcaaactatg acgattcaac ggattcgctc cgcccataga
    41761 attgatcaaa atttttcggt aaatggcatc ttcttcgttc agataatgta cattacctgt
    41821 tgcaacaacg attttatcca aactttttcc gatttctacc aaattttgga taatttcttc
    41881 caaatcatgt tcatttttta ccaattcttg ctcgatcaaa ggcgcataga ccgcttttgg
    41941 cataacttca atataatcgt agaattttgc tcggtttctt gcctcttcta cccctttttg
    42001 catcatggct tcgaatattt ctcccttatc acaagctgag ccgatcaata gattttcacg
    42061 catctttttc aattgggagc gtggtattcg tgggacgcgt tcgaagtatt cgacattcga
    42121 cattgaaatc agtttgaaca aatctttcag tcctgcctga ttttttgcta aaatcgttac
    42181 atggaaaggt cgagcccttt tgtaagaatc tccttctccg atatgctcat ttagctgatc
    42241 atgataaagc atgttatgat tgtccattgc ctctttcaca aagatccatg ctaagtgacc
    42301 agttgcttca gcatcataga ttgctcggtg atgctgttcc aagctgacac caaatttttt
    42361 agataacaca cctaatccga aacgtttgaa ttgaggatac agatatctag ctaattccaa
    42421 cgtatcaatg actggattgg ccgcttcagg aataccatac cttgcgtagc tagtattcaa
    42481 gaaccccata tcaaaagccg cgttgtgggc taccaaaatt gtatcttttg aaaattcaag
    42541 gaacatccgc aatacttctt cttccgactt tgatcctcga accattccat ccgtgatacc
    42601 agtcagatct accgttgttc gtgacaaagg atgtcctgga tcgataaatt catcaaagct
    42661 ttcgatcaca tttcctttat acattttgac tgcggctagc tcgatgatcg tgtcatatac
    42721 tgcagacaat cctgtcgttt ccacgtcaaa tacgacatat gtggcttcac tcaatgcttc
    42781 atgtgcatca ttataagcaa ttgggacacc atcatctacg acattcgctt ccacgccgta
    42841 taagattttc acacctgctt tttttccagc actatgtgct tccggaaagg cttgcgcacc
    42901 accgtggtct gtaatggcga tagctcggtg tccccatttc cctgcttgtg ccacaagatc
    42961 agagatgctg tttgttgcat ccatcgtact catattgcta tgaacatgaa gctctactcg
    43021 tttttcccct tcaggagcat aatctttgcg aggtgtatgt ttgacttcta tgatatcttg
    43081 cgcattcatg accaaatcac ggacaaacgt atcttcttga atacttcctc gtacctttaa
    43141 ccagctaccg acactgatcg catcaaatac ttgttcatct ttttcgccgt tcgagaattt
    43201 tttgacgata aatgatgaag tataatctgt gattttcaaa gtcaagatct tgcgttttga
    43261 acgtagttct ctgacttctt tgtcgaatac gtacccctca agtgtgactc ttcgttcttc
    43321 ttcaacgata ctgatcattg gtgtcgttgg ttcatcggct ggaatatttc gtcctagctg
    43381 gatgtggatg ggtccttcaa gagctggcga ttgctctttt ctttcctttt tcttttgttc
    43441 gtggacaact agactttcag ctgcttgctt cataaaggct tctgcctgtt cttgtttgcg
    43501 ttcttcaaaa agtttcaata ctcgttccgc ttgctgctca tccacttctg gttcgatacg
    43561 aaattttggg aatccgtaac tgacgaacaa ctcttctact aatggcaagt attgttgctt
    43621 caaataagaa atagctcctg tactgtcgat tggcaagatg actttacgat cttttatcac
    43681 aggtacctgc gttttcaata cttgctgtgc tagtggtgta tcgcattgct gattttccaa
    43741 agcttgggcc cagtaatctt gcaagagctg ttcatcgaat gtctggtcat ctgctaagat
    43801 attcagtttg acttgtgcaa tatctttaaa agcgatagtc agatgctgag tcaatgaccg
    43861 atatagcata ataggcaaaa ttttggaaaa atgcagcgta aattcccata aacgcgattt
    43921 acgatggacc actacactat ccatttcacc agcagtaagc agtggatgat ttttttctgc
    43981 ttcctctagt tggatttgtt ccaacagttt ttcaaataaa tctcgttttt ctgacaaaga
    44041 tatacgccct ttctgatgag aaacaatgca attgatcgca tcttatcctc ttctttttca
    44101 gtacctttta gtatactcgt tttttctgag ttttacatta aaaaattgga actgcgattc
    44161 aaatcgcagt tccaatttta aaagcgaatc atcagattgt caaaaacagc tgattcagct
    44221 atttttattt tctgtgtttt aacgcttatt attcgccttg gttcaagagg attggtaaag
    44281 tattcaccaa ttcttcttta cgtacttcaa ccatttcgcc agttttcttg attttcactt
    44341 ctacgattcc atccactgct tttttaccga cagtgattct gattggacag ccaatcaaat
    44401 cagaatccgc gaatttcact cctgcgcgtt catttctgtc atcaaccagt acttcatagc
    44461 cagcttttgt catcgcttct tctagttcat tcgtcaggtt tgtttggtat tcgtctttta
    44521 gattcatttg gacgatatgc aaatcaaatg gcgcaattcc ttttggccag ttgattcctt
    44581 gttcatctga atgttgttct acgattgctg ataataaacg acttacaccg ataccataac
    44641 agcccataat cacaaatttt tctcgtccat tttcgtctag tacagttgct cccatagatt
    44701 cactgtaacg agtgccgatt ttgaagatat gtccgatttc gattcctcga gtaaaggcaa
    44761 gaacaccatt gttgtctggt gaaggatctc cttcttgtac aaaacgaaga tcttcataag
    44821 aaagtacagt gaaatcgcgt tctggattca catttttata gtggaatcct gtttcgttcg
    44881 ctccagtaat ggcattcgct aaatcttgga cataacggtc tgcatatact tttacgtcat
    44941 ctgaaacacc gactggtccg acagaaccaa actcagcacc tagatatttt tgtgcttctt
    45001 cttctgttgc ttcttctaaa aagtcagcag ataaaaagtt tttcagtttt acgtcattga
    45061 cttcatggtc tccgcgtacc aaaacaagga caggttgttc atccgcaatg aacaatacag
    45121 acttgatgat tttttgaggt tcaacagaaa agaaatctgc tacttcgtca attgatttta
    45181 cgttaggtgt agctactttc tccaattcaa gctgtgtttc atgcgatttt tttgatacat
    45241 agtagcttgt agccatctcc aagtttgcag cataatcact ttcagtcgaa taacagatcg
    45301 tatcctcccc gatttctgag atcgccatga attcttttga gtctttcccg cccattgcgc
    45361 caccgtctcc gataatcgag cgaaattcta agccacaacg cttgaatact tcggtgtagg
    45421 ctttttcgta atctttatat gtctcgtcca aactttcttc cgttgcatgg aaagaatacg
    45481 catctttcat gataaactct cttccgcgaa gcaaaccgaa gcgaggtcgt ttttcgtcac
    45541 gatatttagg ttggatttga taaagattta aaggtaattt cttatatgaa ttgatttcat
    45601 tacgcatcaa atcagtaaaa gtttcttcgt gagttggtcc aaggatcatt tttcgatcat
    45661 tgcggtcttt gaaacgatac aagtttggtc catatgtttc gtaacgcccg gattcttccc
    45721 aaagttctgc tggcaagatg gcaggcatca gcatttctac cgctccgatt ttttcaaatt
    45781 cttcccgcat aatgcgtttc agtttttcca atacacgatt tgctaatggc agatatgaat
    45841 aaatacccgc tgatacttgg cggatatatc cagctcttag caagatttga tggcttaata
    45901 cttctgcatc atttggaact tcgcgtaatg ttggaatcaa catttttgat tgtctcattc
    45961 tatatactcc tttatttcct caaaatagtt atcgtttgtt attataccct atttgtcaac
    46021 tttttctaga aaaagaatcg ttgaatatcg ttccatgtaa ctagcaccat caacaacatt
    46081 aaaaatccaa agccagctaa tgtcaatatt ccttcttttt cttgacttaa tggtttcccg
    46141 cgaatccctt caaaaatatt caagactaat ttcccgccat ctaaggcagg aatcggaagc
    46201 agattgacga ttccgagatt cattgaaaga agcgccatca aactaataac agttgttaca
    46261 ccttgatttg ctgcttctga agacaattgg tacatcatga caggtccgcc taatttatct
    46321 aaactgaaac ctgtaaatag tgatcctaag gctttgaaaa tttccaatga accgctgaaa
    46381 gcttgacgcg tccctccaat gatcttatcc ataaaacctg tattcattgg ggcttttata
    46441 ccaagttggc caactttttc gccatttgac tctacagatt tcggtgtcac gtcgacagat
    46501 tgcacctgac cttctcgttc gatcttaaag tctaatgttt tatccggatt tttcgtaatg
    46561 actgttgtca gatcattcca tgaatggatt tcttttccgt ccacactgac tacctcatca
    46621 ttttctttta agcctgcttc tgccgcagct ccattaggca tgatctctcc tacgcgatta
    46681 gtgttcgtca cttgaacacc accttgcata aatgccagaa tgatgaataa caggattgcc
    46741 aaaataaagt tattcatggg tccagcaaag ttggtcagca tacgctgcca caatttagcc
    46801 gattggaact gtacgtcttt tggcgcaatt ctgatttctg ttccgtcgtt ttcaataatc
    46861 gtcgcatcgt gatcaacagg gtaggttact acttctgttt cgtctccatt cacataacca
    46921 gtgatagtca gctcatcttc taaatcataa cggctcaatt ccatcgggat agcatttgtc
    46981 aattggattt tcttacttaa atttatcttc tctacgatgc cgtcagaatt tagtaaaaga
    47041 gaaagaggca ttcctggtgc catttctgtt tcatcatccc cattgcctgc cattcgaaca
    47101 taaccgccaa taggtaatag gcgaagcgta tatgttgtcc cgtcttttgc ttgatgccca
    47161 taaattttcg gacccatacc gatagcaaat tcgcgaacga ggattcctga tcgtttcgca
    47221 aagaagaaat gaccaaactc atgaacaatc actaatatac caaaaacgat gataaatgtc
    47281 agaatcgttt tcatagcttt tttctccttt acttgttttt tgtgtttttt ataaaacaac
    47341 gataaaacac tataatttta ccatagtata ccaaagacac caatagtatc aatataatga
    47401 aaaataaaaa cctgcgtctt ttatgaaatt cacaaagaaa cgcaggtata cttactaaat
    47461 aatgccaaat aaatgcatga taggaaatac aaacagcaaa ctgtcgaaac ggtctaagat
    47521 tccaccatgg cctggtaaga tatttcccga gtctttcaca tcataatgac gtttgattgc
    47581 agattcgacc aaatcaccaa attgtccgac tgctgaaaaa ataatcgtca tcagaagcat
    47641 gacaaacata ccatgattaa atagttcttt tgctgggtat agcaataaat aaagaaatgc
    47701 gactacaaca gcactaccaa taccaccgag cgcaccttca atcgttttgt tgggcgaaat
    47761 atcaggactt aacttatgct gaccataacg tcgaccaacc atatatgctc cgatatcagt
    47821 tgcccaaaca ataaatagac caaatatcaa aacagccaag ctttctgttc gtgcattgac
    47881 aaaattctgg aaacctattc ccacatacaa gctgacaagc acaggaaaac ctgcttcatc
    47941 aatcgtatac gtatttttag aaataactga tgttcctaaa agaatcataa ctataagata
    48001 aaataacata aatgtactta cattctctgg aagaaagaaa aaccatcttt cttttggcaa
    48061 aacaagtaac actgcaccaa cggcggataa aactccttca aaacttaaga gtgtcaatcc
    48121 tttcatccga aacagctcgt aaactcctat tactgctaag attgctgctg taagctcaac
    48181 cgctatgcct ccgatccata taatcggaat aaacaagact agcgccacgg ctgctgtgac
    48241 cacacgttgt ttcatgactg atcccctttc tcatcttctg ttttttttac accgccgaat
    48301 cgacggtctc gattttgata agaggcgatt gcttcttcaa gatgcgcacc atcaaaatca
    48361 ggccaaagcg cttttgtaaa atacagctca ctatatgcga tctgccataa cagaaaatta
    48421 ctaattcgct cttctccact tgtacggatc atcagctcag gatcttgtaa ctctttaggc
    48481 aagaaacctg tcattaaatg cttagcaatc aattcatcat cgatttcttc tgcgtgtatt
    48541 tctccttttg ctacttcttc ggcaatttct tggacagcag tcacgatctc tgctctgctt
    48601 ccataattca aagcaaaatt caataccatt ccagtattgt tttttgtttg ctcaattgca
    48661 cgtcttacag catcttgcgt atgttctggc aatacgtttt cataacccat gacatgaact
    48721 ttgacatttt ctttaatcaa ttctggtacg aacgtatcaa agaagtcgac aggtagttgc
    48781 atgagaaaac ttacttcatc ttttggtcgc ttccagtttt cagtcgaaaa ggcataaagc
    48841 gtcaacactt tcactccaag ccttgatgct ttttttgtga ctttctttac tgtttccatg
    48901 ccttctttat ggccagcaac tcgtggcaaa cgacggtttt gcgcccaacg accattgccg
    48961 tccatgatga tggcaatgtg ctgaggaatt ggtccatcgg tattaaattg aaattcgctt
    49021 ttttcttgaa tatatttgtt tttttgcgga aaaaaacgta acattctttt tcctcctata
    49081 cttaaaagat tcccactatc ttaagtatta tagcaatatc taactgtttt tcctatgaaa
    49141 tcattgtttt tttatttaaa aactgaataa ttttttcttt ttttaatttt taaataaaag
    49201 aaatataaat aaaaacagag gaagcggaat gatccgcccc tctgttttta tgattttatt
    49261 aaacttccaa taattctttt tctttatcag cagcgatctt atctacttct ttgatgctgt
    49321 tatctgtcaa tgtttgtacg tctttttcta atccgcgcaa atcatcttcg gtaatatcac
    49381 catttttttg ttgtttttta tattcatcga tcgcatcacg acggatgtta cgaatcgcga
    49441 tttttgcatt ttctgcttct tttttaacat ctttagctaa ttcttttctg cgttcttctg
    49501 tcagttgcgg gataactaag cgaatgacat ttccatcatt ggttggactg attccgatat
    49561 cacttgccat aatagctttt tcaacatctt gaagaatgct cttgtcaaaa ggtgtgatca
    49621 ttaaaacacg tgcttctgga atttggattg atgccatttg gttcaatggt gttggcgcac
    49681 cgtagtagtt cacagtaata cggtccaaaa ggctagcgtt cgcacggcct gcgcggattt
    49741 gcccaagttc acgttgaaga ttttgtgctg cttttttcat tttttctttt gcttctgtca
    49801 taattgcatc tgccattttt atttccccct tacagttgtt ccgatatttt cgcctaagat
    49861 tgcacggcga atatttccgt gttcatttaa attgaagaca agcagtggaa tatcattatc
    49921 catgcttagt gaacttgcag ttgaatccat gacttgtaat cctttggcaa taacttccag
    49981 atgagtcagt tcttcaaatt ttactgcatt tgcatctact ttcggatcag cagaataaac
    50041 accgtctaca ttgtttttag ccatcaggat aacatctgcg ccgatttctg ccgcgcgtaa
    50101 tgctgctgtt gtgtctgtcg agaagtacgg gttgccggta ccgcctgcaa agataacgat
    50161 ccgtcctttt tctaaatgac gttctgcttt acggcgaata tatggctctg cgatttgacg
    50221 catttcaatc gaagtttgca cgcgtgtcgg tacacccacg ttttccaacg tatcctgtaa
    50281 agctaaggca ttcataactg tagctaacat acccatgtaa tcagcttgag cacgttccat
    50341 gcccatttgt gctccgattt gtccgcgcca gatatttccg ccgcccacaa cgattgccat
    50401 ttcaacgccc agctcgtgaa cttctttgat ttcttggatg atttctttga ttactggtgg
    50461 tttgattcca aatcctgcgt ccccagctaa agcttctcca ctcaatttta agactacacg
    50521 ctgatatttc ggtgtgacca tgttcattcc tccaatattt tctattgcca ttttaccata
    50581 cttagatgat ttcgacactc taagactggt ttttcttgtt ttgcttaaaa aaagggagcg
    50641 cacaaaccgt gcgttcccgt ctgttattca acagaattat tttttcactt ggctcataac
    50701 ttcttctaca aagttatctt cgcgtttttc gataccttcg ccaacttcga aacgtacgaa
    50761 tgttttaaca gttgcgcctt ttgatgcaac gtatttttca acagtcatat caggatcttt
    50821 aacgaatggt tggtccacca aagcgatttc tgctttgaat ttcttcaagc gtccttctac
    50881 cattttttca acgatattcg ctggtttgcc ttcgttcaat gcttgttctg taagaacagt
    50941 tttttcatgt tctaattcag cttctggaat ttgtgattcg tttacataac gaggattgat
    51001 agctgctacg tgcatagcta cgtctcttgc aactgtttcg tcagttgttc catctagaac
    51061 agtcaatacg gcaatacgtc cgcccatatg caagtatcca ccaaatgcag cattgtcgtc
    51121 tttttctaca acttcaaagc gacggaagct gattttttct ccgataactt gagttgcttc
    51181 gatcaagtca gattcaattg tacctttatc tgttttgatt ttcattgcag cttccatgtc
    51241 tgctggtttg ttttcagcaa caagttctgc aatttctttt actaaatctt ggaacatttc
    51301 gtttttagaa acaaagtctg tttctgagtt tacttcaacg attgctgcag tgttaccttt
    51361 cacagcaact gaagccaatc cttcagctgc aatgcggtca tttttcttcg cagcctttgc
    51421 cattcctttt tcacgtaaaa gatcaactgc tttttccatg tcgccttcta cttcaactaa
    51481 tgcttttttc gcatccatca ttcctacgcc agtcatttcg cgtagttctt ttaccatttt
    51541 agctgtaacg tctgccatgt ttaactcctc ctgtgtgttt ttctttttaa aaaaagctgc
    51601 ttcaaaggag agtgaggctt gtcgcccagc ctttgaaaca gctccatcat tcaaaattat
    51661 tctgctgaag aattgtcgcc ttcaacaaca tctacgattt cttcgattga tgttgctgtg
    51721 ttttcttctg tgaaatcttc ttcaactact tgatcttcac cttggtttcc ttcgatgaaa
    51781 gcatcagcca tttttgaagt aatcaattta acggcacgaa ttgcatcgtc gtttgaaggg
    51841 attactacgt cgatttcatc tggatcgcag tttgtatcaa ccatagctac gattgggata
    51901 tttaatttgt gtgcttcttg aacagcaata cgttctttac gtgggtcaac gatgtacatt
    51961 acatctggga ttcttggcat atcagcgata ccgcctaaga atttttcaag acgttcacgt
    52021 tctttgttca aaccagcaac ttctttttta ggtagaactt caaaagttcc atcttcttcc
    52081 attgcattga tttgtttcaa acggctgata cgtttttgga tagtatccca gtttgtcaat
    52141 gttccgccta accaacgatg gttaacgaag tattgtcctg cacgagttgc ttcttctttg
    52201 attgcttctt gtgcttgttt ttttgtacct acgaataatg ctacgccgcc ttcttcagca
    52261 acgctcttca tgtaatcata agctgcatct actaatttaa ctgttttttg caagtcaatg
    52321 atgtagattc cgtttctttc tgtgaagata tatttcttca tttttggatt ccagcgacga
    52381 gtttggtgac caaagtgtac gccggcttct agtaattgtt tcatagaaat tactgccatg
    52441 tttgtttcct ccgatttggt tttatttttc ctcttcttat cttctacctt gccggcaaac
    52501 tttttcaagc acctaccgac aatccaataa gaatgtggat ttgctgatgc ataaagcacc
    52561 ctcagctatt atacagacaa aagatccgta tcgcaagagc aaatatactt ttctgctcaa
    52621 cattcaatag tttacctgaa agataaaaag aattcaagaa aaacagtaga tttctctttg
    52681 tttctttatt atcctttttc cataaaataa ttttaggtat aaaagacgaa tattattggg
    52741 caatccttct tttttttgtt atgatacatg tgaaatcttt cgtaaaggag aaagaaaatg
    52801 cgcaaagctc aaagacattt gctgattaaa caagtcattg aagaattccc gatccgtacg
    52861 caagaagaac tattaaacaa acttgggcat tatggcgtta ccgccacgca ggcaacgatt
    52921 tctcgagata tccgtgatct aaaaatcgtg aaggcacctg atgaaaatgg cgtttcccgc
    52981 ttcgtattgt ttcgcgaaaa aggaactcct gagactagga ctgaggaaga ggatcggctg
    53041 gttcaaatga ttgaagacat tgtattgaaa gtcgaacgag tccacttttt gacgatcgtc
    53101 aataccttgc cagacaatgc tcatttattt gcatctgttt tagatgagat caaacctccc
    53161 tctattgtca gtacaattgc tggattcgat acgatcatgg tcatctcgga aaatgaggag
    53221 gacgcaaaac gagtagaaga atttcttcac catccaactg aagtatcact tttttaatca
    53281 gagcacgtat tcctaatatg gaatgcgtgt ttttttattt tcaatatttt ccttattgaa
    53341 taaaccttta cactctttac aaaaatacat cattttttat tcaaattttt caacaccttg
    53401 attgaatcac aataatcatc atttatattg gatgttaaca agagcagctt cttgctcttt
    53461 gtgtatttca aaaaagaaat gaaaggaaat catatgtata aattattaac ttctggacca
    53521 ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg atcattgtac ttgggatcaa
    53581 gaatacaaag aaattacaca atggataaga aaaaaattat tggatcttgc tcaagtatcg
    53641 gaaggaatat acacaacggt cttaatgcaa ggtagcagct ccttcggggt agaatcggta
    53701 ttaagttctg tgatcaggcc agaagatact gtactgattt gttcaaacgg cgcatataga
    53761 caacgaatga tcaacatgtg cgaatgtctg aaactgaact ataccgttta tgaggtatcg
    53821 gaacatcaaa tcctccaagc tcatgtgatt acacagctga ttgaagagaa tccgtctatc
    53881 tccgcactag caatggtcca tagcgagact acttccggta ttttgaataa tttggaagag
    53941 attgctgctg tcacacaaga aagagatctt ttgtttatag tggacgcgat gtcaagcttt
    54001 ggtgggatac cgatttgtgt agccgaacta gggattgatt ttttgatttc cagctcaaac
    54061 aaatgtattc aaggtgttcc aggcttttct tttgtacttg ctaataaagc ggaactggaa
    54121 aaacgaaaaa atgttagccg aagtttgtca cttgatctat atgaccagta ccgcgtgatg
    54181 gaaaacgaga atggcaaatg gcgtttcact tcccctaccc atacggtttt ggcttttcaa
    54241 caagcgctga aggaactaga ggcagaagga ggaatcgaaa ctcgatattc acgttatctc
    54301 gccaataatc atgtgctacg acaaaaaatg acagagatgg ggttccagcc tttcattcaa
    54361 gaatatcagg ggccattcat cacctctttt ctttacccta aggactggcg atttgatttt
    54421 aatcattttt atcagtattt aaagcaaaat ggatttgtga tctatcccgg gaaaatcagc
    54481 gatcatgaat gttttcgtat cggaaatatt ggcgagatct atccggaaga tattttggaa
    54541 ctgacagcaa ttatcaaaga ctataaggag aacgttaatg attaaaactg taatttttga
    54601 ttgggcagga acaactgtgg actttggctg tatggctcct gtccacgctt ttagaaatgc
    54661 ttttctagaa aaaggaatcc aactgactga caaagaaatc cgctaaccga tgggaaaatt
    54721 aaaatgggat catatccaac agcttctttc cctcccttct gtcaaaaaac aatggatagc
    54781 tatttatgga caactacctc aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca
    54841 ttatctgttt aaagatttgg tcaagcattc taccttgaaa cccgacacat tggaaacgat
    54901 cgaacggttg agaaacgctg ggatcaatat tggttccacg aatggattta atgctcctat
    54961 gatgacaatc gtagcagaaa ccgcaaaaaa agaaaggtat tcaccagact tcatggcaac
    55021 ttttgaagac gtcgaagggt atggtcgacc gtttccttat atgatccata aaaacatgca
    55081 gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc ggagatacgg tctcagatat
    55141 ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta attgaaggta gttcgctaat
    55201 ggggatttct tatgatgagt accaaaaatt agagatgggg aaaaagatcg tttaaaacag
    55261 tctgttgctc ttagattcca acaagccggc gctgatattt gtgtaaacaa tttaaaagag
    55321 ttaacagaat acattttgta cgctgacatg aatccagaat gacttttata caaaataaga
    55381 ggttgtgaaa aagctgtcct gtatcaagta ataaaaacag ccaagcaaaa atagctcctc
    55441 atatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttattc
    55501 gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
    55561 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
    55621 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
    55681 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
    55741 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
    55801 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
    55861 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
    55921 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
    55981 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
    56041 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
    56101 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
    56161 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
    56221 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
    56281 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
    56341 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
    56401 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
    56461 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
    56521 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
    56581 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
    56641 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
    56701 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
    56761 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
    56821 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
    56881 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
    56941 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
    57001 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
    57061 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
    57121 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
    57181 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
    57241 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
    57301 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
    57361 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
    57421 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
    57481 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
    57541 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
    57601 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
    57661 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
    57721 ctagtcgttt cataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat
    57781 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
    57841 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
    57901 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
    57961 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
    58021 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
    58081 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
    58141 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
    58201 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
    58261 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
    58321 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
    58381 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
    58441 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
    58501 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
    58561 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
    58621 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
    58681 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
    58741 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
    58801 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
    58861 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
    58921 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
    58981 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
    59041 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    59101 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    59161 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    59221 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    59281 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    59341 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    59401 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    59461 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    59521 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    59581 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    59641 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    59701 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    59761 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    59821 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    59881 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    59941 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    60001 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    60061 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    60121 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    60181 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    60241 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    60301 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    60361 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    60421 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    60481 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    60541 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    60601 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    60661 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    60721 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    60781 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    60841 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    60901 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    60961 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    61021 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    61081 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    61141 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    61201 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    61261 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    61321 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa ttt
//
LOCUS       DAFCNS010000005        59806 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFCNS010000005 DAFCNS010000000
VERSION     DAFCNS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.005"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(233..1081)
                     /locus_tag="LNE23_000310"
     CDS             complement(233..1081)
                     /locus_tag="LNE23_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6930190.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="LNE23_000311"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="LNE23_000311"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBK6930191.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="LNE23_000312"
     CDS             complement(3582..3761)
                     /locus_tag="LNE23_000312"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBK6930192.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="LNE23_000313"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="LNE23_000313"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK6930193.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGVKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="LNE23_000314"
     CDS             5909..8188
                     /locus_tag="LNE23_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6930194.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETEIDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="LNE23_000315"
     CDS             complement(8281..8928)
                     /locus_tag="LNE23_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930195.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="LNE23_000316"
     CDS             complement(9107..9826)
                     /locus_tag="LNE23_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6930196.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="LNE23_000317"
     CDS             complement(9957..11108)
                     /locus_tag="LNE23_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBK6930197.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="LNE23_000318"
     CDS             complement(11325..12065)
                     /locus_tag="LNE23_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6930198.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12425)
                     /gene="rsfS"
                     /locus_tag="LNE23_000319"
     CDS             complement(12069..12425)
                     /gene="rsfS"
                     /locus_tag="LNE23_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBK6930199.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="LNE23_000320"
     CDS             complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="LNE23_000320"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBK6930200.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13676)
                     /locus_tag="LNE23_000321"
     CDS             complement(13026..13676)
                     /locus_tag="LNE23_000321"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBK6930201.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="LNE23_000322"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="LNE23_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBK6930202.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="LNE23_000323"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="LNE23_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBK6930203.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="LNE23_000324"
     CDS             complement(15119..15646)
                     /locus_tag="LNE23_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBK6930204.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15873..16658)
                     /locus_tag="LNE23_000325"
     CDS             complement(15873..16658)
                     /locus_tag="LNE23_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBK6930205.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(16651..17520)
                     /locus_tag="LNE23_000326"
     CDS             complement(16651..17520)
                     /locus_tag="LNE23_000326"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBK6930206.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHML
                     LELHTQKGWIDRG"
     gene            complement(17521..18900)
                     /locus_tag="LNE23_000327"
     CDS             complement(17521..18900)
                     /locus_tag="LNE23_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBK6930207.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPTRHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18908..19327)
                     /gene="fabZ"
                     /locus_tag="LNE23_000328"
     CDS             complement(18908..19327)
                     /gene="fabZ"
                     /locus_tag="LNE23_000328"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK6930208.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19352..19828)
                     /gene="accB"
                     /locus_tag="LNE23_000329"
     CDS             complement(19352..19828)
                     /gene="accB"
                     /locus_tag="LNE23_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBK6930209.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19831..21066)
                     /gene="fabF"
                     /locus_tag="LNE23_000330"
     CDS             complement(19831..21066)
                     /gene="fabF"
                     /locus_tag="LNE23_000330"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBK6930210.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21085..21822)
                     /gene="fabG"
                     /locus_tag="LNE23_000331"
     CDS             complement(21085..21822)
                     /gene="fabG"
                     /locus_tag="LNE23_000331"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBK6930211.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21834..22751)
                     /gene="fabD"
                     /locus_tag="LNE23_000332"
     CDS             complement(21834..22751)
                     /gene="fabD"
                     /locus_tag="LNE23_000332"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK6930212.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22858..23082)
                     /locus_tag="LNE23_000333"
     CDS             complement(22858..23082)
                     /locus_tag="LNE23_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK6930213.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23135..24097)
                     /locus_tag="LNE23_000334"
     CDS             complement(23135..24097)
                     /locus_tag="LNE23_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBK6930214.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24107..24547)
                     /locus_tag="LNE23_000335"
     CDS             complement(24107..24547)
                     /locus_tag="LNE23_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6930215.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24853..25819
                     /locus_tag="LNE23_000336"
                     /pseudo
     CDS             24853..25819
                     /locus_tag="LNE23_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(25876..26106)
                     /locus_tag="LNE23_000337"
     CDS             complement(25876..26106)
                     /locus_tag="LNE23_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBK6930216.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26192..27079)
                     /locus_tag="LNE23_000338"
     CDS             complement(26192..27079)
                     /locus_tag="LNE23_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBK6930217.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27168..28391)
                     /locus_tag="LNE23_000339"
     CDS             complement(27168..28391)
                     /locus_tag="LNE23_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6930218.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28408..29454)
                     /locus_tag="LNE23_000340"
     CDS             complement(28408..29454)
                     /locus_tag="LNE23_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6930219.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDVEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29618..31345)
                     /gene="ptsP"
                     /locus_tag="LNE23_000341"
     CDS             complement(29618..31345)
                     /gene="ptsP"
                     /locus_tag="LNE23_000341"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBK6930220.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31345..31611)
                     /locus_tag="LNE23_000342"
     CDS             complement(31345..31611)
                     /locus_tag="LNE23_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBK6930221.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32090..34324
                     /locus_tag="LNE23_000343"
     CDS             32090..34324
                     /locus_tag="LNE23_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6930222.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34472..34792
                     /locus_tag="LNE23_000344"
     CDS             34472..34792
                     /locus_tag="LNE23_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBK6930223.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34997..35221)
                     /locus_tag="LNE23_000345"
     CDS             complement(34997..35221)
                     /locus_tag="LNE23_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBK6930224.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35419..36999)
                     /locus_tag="LNE23_000346"
     CDS             complement(35419..36999)
                     /locus_tag="LNE23_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBK6930225.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37400..38776)
                     /locus_tag="LNE23_000347"
     CDS             complement(37400..38776)
                     /locus_tag="LNE23_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK6930226.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38904..40022)
                     /locus_tag="LNE23_000348"
     CDS             complement(38904..40022)
                     /locus_tag="LNE23_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6930227.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            40164..40444
                     /locus_tag="LNE23_000349"
                     /pseudo
     CDS             40164..40444
                     /locus_tag="LNE23_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(40522..41055)
                     /locus_tag="LNE23_000350"
     CDS             complement(40522..41055)
                     /locus_tag="LNE23_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBK6930228.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41149..42327)
                     /gene="mutY"
                     /locus_tag="LNE23_000351"
     CDS             complement(41149..42327)
                     /gene="mutY"
                     /locus_tag="LNE23_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBK6930229.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42320..43117)
                     /gene="recX"
                     /locus_tag="LNE23_000352"
     CDS             complement(42320..43117)
                     /gene="recX"
                     /locus_tag="LNE23_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBK6930230.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43224..44606
                     /gene="rlmD"
                     /locus_tag="LNE23_000353"
     CDS             43224..44606
                     /gene="rlmD"
                     /locus_tag="LNE23_000353"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK6930231.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44780..45358)
                     /locus_tag="LNE23_000354"
     CDS             complement(44780..45358)
                     /locus_tag="LNE23_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBK6930232.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45680..46150
                     /locus_tag="LNE23_000355"
     CDS             45680..46150
                     /locus_tag="LNE23_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBK6930233.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46258..47484)
                     /locus_tag="LNE23_000356"
     CDS             complement(46258..47484)
                     /locus_tag="LNE23_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6930234.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48238..48438)
                     /locus_tag="LNE23_000357"
     CDS             complement(48238..48438)
                     /locus_tag="LNE23_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6930235.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48572..49180)
                     /locus_tag="LNE23_000358"
     CDS             complement(48572..49180)
                     /locus_tag="LNE23_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBK6930236.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49265..49735)
                     /locus_tag="LNE23_000359"
     CDS             complement(49265..49735)
                     /locus_tag="LNE23_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBK6930237.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49783..50697)
                     /locus_tag="LNE23_000360"
     CDS             complement(49783..50697)
                     /locus_tag="LNE23_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK6930238.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50843..51646
                     /locus_tag="LNE23_000361"
     CDS             50843..51646
                     /locus_tag="LNE23_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBK6930239.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51824..52771
                     /locus_tag="LNE23_000362"
     CDS             51824..52771
                     /locus_tag="LNE23_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBK6930240.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52839..53132)
                     /locus_tag="LNE23_000363"
     CDS             complement(52839..53132)
                     /locus_tag="LNE23_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBK6930241.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(53160..53825)
                     /locus_tag="LNE23_000364"
     CDS             complement(53160..53825)
                     /locus_tag="LNE23_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBK6930242.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(53965..54597)
                     /locus_tag="LNE23_000365"
     CDS             complement(53965..54597)
                     /locus_tag="LNE23_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6930243.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54604..55671)
                     /locus_tag="LNE23_000366"
     CDS             complement(54604..55671)
                     /locus_tag="LNE23_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6930244.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(55668..56399)
                     /locus_tag="LNE23_000367"
     CDS             complement(55668..56399)
                     /locus_tag="LNE23_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBK6930245.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(56567..57046)
                     /gene="greA"
                     /locus_tag="LNE23_000368"
     CDS             complement(56567..57046)
                     /gene="greA"
                     /locus_tag="LNE23_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBK6930246.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57182..58357)
                     /gene="mltG"
                     /locus_tag="LNE23_000369"
     CDS             complement(57182..58357)
                     /gene="mltG"
                     /locus_tag="LNE23_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBK6930247.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            58522..59682
                     /locus_tag="LNE23_000370"
     CDS             58522..59682
                     /locus_tag="LNE23_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK6930248.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT        17325 a        12592 c        10075 g        19814 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt ggacgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gattggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgttta gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctgga gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttact ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctgatcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtaacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgtgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaat
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggtcgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattgcc
     7021 tttacatcat agactctctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag atggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aaacgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcacat tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgct tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccaa catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaataagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctcgt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttgt ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaatttc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ctgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctagc
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaacttcg ttctggtgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaatgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca accgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatttct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctctggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc acagctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttctgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtta atgagacatc ttggatctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaag cgagcaagac
    15841 cattctttac tctttgctta gttgttaaac acctaatatt ggcggaacct cttgtatcgt
    15901 ttttccagta attcatgctg gtcgaatcga acaagttccg tcaatctttc aatcaacgct
    15961 tttttcataa tgtgtgcgac tttatatcgt ggcaactgct cgccgttgaa cgtttcagga
    16021 ataactcgat caacgattcc taactcgcgt agttctggcg cagtaatttt catcaactca
    16081 gctgcttctg cggcgcgact accgtctttc cacaaaatcg acgcaaatcc ttctggagaa
    16141 agcacagcat aaatcgtttc ttccagcatc cagacttcat ccgctaaacc tagagcaagg
    16201 gcaccgccac ttccaccttc accaatgatg atggcgatga tcggtacctt taaatcagac
    16261 atttcgagca gatttctggc aatagcctct ccttcgccgc gctcttctgc tcctacaccg
    16321 caatatgcac ctgctgtatt gatcaatgta atcaccggtc tattgaactt ttccgcttgt
    16381 ttcatcagtc gtaaagcttt tcgatagcct tcaggatggg gagaaccaaa atttctgcgg
    16441 atattttcct gcagatcccg tcctttttga ataccgatca ctgttaccgg tttgccatct
    16501 aatcgagcga ttccaccaac aatcgcttcg tcatctgcaa acaaacgatc accatggcat
    16561 tcctcgaaag aatcgaagat ctcttcaaaa aaatccaagc ttgtccatcg ttctttagct
    16621 cttgctaact gaacgacatc atgtgctgtc ttatccacga tctatccatc ctttctgtgt
    16681 atgcaattca agaagcatat ggattttctg cttcaattcc atgcggggaa caatctgatc
    16741 gacaaaacca tgagataata agaattcagc cttttggaaa tcttctggaa gttcctgctt
    16801 gatcgtctgt tcgatgacgc gtcttccagc aaaaccgatc aagctttggg gttctgctag
    16861 aatgatatcg ccttccattg caaaactggc agttactcct ccggtcgtcg ggtccgttaa
    16921 aacagttaga taaaaaagac cttcattgct atgccgtttg acagcagcag agatttttgc
    16981 catctgcatt agagaaaaga ttccttcttg catccgtgcg ccacctgaag ctgtgaacaa
    17041 gacgactgga agactttcct ttgttgctcg ttcgaataat ctggtgatct tttctccgac
    17101 aatcgttccc atacttccca tgataaaatt aggatccatc actccaataa tgaagggaat
    17161 cccttgaatg gttgctttac cagtgaggac tgcttcatga agtcctgttt tatcttgcat
    17221 ttttttaatt ttttccgtat attcgggaaa tgctagcgga tctttggtta caagatccgt
    17281 atcccattct tcaaaacttt tttcatcaat aatcaaagct agacgctgcc atgcaccgat
    17341 tcgaaaagcg tatccgcaat gagggcatac tttttcttca ccgatgtctt tcgtataaag
    17401 gatatgtttg caatttggac actttgccca catgttatca ggaacagatg gcgagttagc
    17461 aggtttgctc tctctattag gattgatccg aatatatttc tttttcttaa acagagccat
    17521 ctatgcgcct cctatctccg tttctggtgt ccaattcggt aaaaattctt cttgtaaaaa
    17581 tgctgtgctg taatcacctg ctatgactgc agggtgacta atcaaatcca tctgaaattc
    17641 tgcgttcgtg atgattcctt ctgtgataaa ttcacttaag gctcgctgca tcttcattaa
    17701 tgcttcaaaa cgcgtatttc catggacgat tatttttgca atcattgaat cataatatgg
    17761 cgggatcgta tatcccgggt aaactgcact gtctacccgc agtcccatcc cacctgctgg
    17821 aagcagtaag ttttggatct tccctggaga aggagcaaaa tggaaagcag gattttctgc
    17881 attaatccgg cattcgattg cgtgtccttg gaatactact tcttcttgac acacattcaa
    17941 aggctcaccg gctgctatct ccacctgttt ttttactaaa tcgattcctg tgaccatttc
    18001 tgttactgga tgttccactt ggattcgagt attcatttcc atgaaataaa aatcgccttc
    18061 ttcatccatc agaaactcga tcgttccggc attttcataa ttgactgctt tagctgctcg
    18121 aacagcagcg tctcctagtg cttctctttt tgtttgtgag atcactacag aaggagattc
    18181 ttccagtact ttttgattat tacgttgcaa agaacaatct cgttctccta aatgaatgac
    18241 attgccaaaa tgatcaccta aaatctgaac ttctatatgg cgagtaggat aaatgatttt
    18301 ttccaaatac atatcatcat ttccaaatgc cgctgctgct tcttgctgag ctgaagaaaa
    18361 atgctgtggg agctcttctt ttgattgaac tttccgaatg cctttcccgc caccacctgc
    18421 agctgctttc agcatcactg gataaccaat tctgtctgct atttccaatg cttcttctac
    18481 tgtagataat acaccagtac ttcctggaat gactgggaca tttgcttctt gcatcaattg
    18541 acgcgcattg atcttatttc ccattgcatc gatcgttgca cttttaggac caataaatgt
    18601 gatattgcac tcttcacaca tggaggcaaa tcggctattt tcagataaaa aaccaaatcc
    18661 tggatgaatt gcttcggctt ttgtcacgat tgcagcgctc agtacttcct gaacattcag
    18721 atatgaatca acggcttttg ccgggccaat gcagattgct tcatctgcta actgtgtatg
    18781 aagtgcttcg gcatctgctt ctgaatatat cgctacggtt tggatcccta gttcgcggca
    18841 tgcacgaatg atacgtacag cgatttcacc gcggttagct atcaatactt tagaaaacat
    18901 atttcaccta tccaatcata aaggttaatt ccgcttcggc tactttttta ccgtctactt
    18961 tagcgattcc tttgcctatt ccggctgaag ctttcacttt caatatctca acttcaaggt
    19021 ataaggtatc tcccggtgta actttcttac ggaattttgc tttgtccaat ccgccgaaat
    19081 aagccgtctt tcccttgaat tgttcaagag aaagtaatgc aactgctcct gcttgagcca
    19141 tcgcttcaac aattaatacc cctggcataa caggttcttc tggaaaatgt ccttgaaaaa
    19201 agggctcatt gatcgttaca tttttcttag caacgatccg ttctccttca accatttctt
    19261 ccactcgatc aatcaaaagc attggataac gatgtggaat gatttctttt atttcttgta
    19321 tgttcattga tttccctctt tcctgctttg cttatttttg ctgaatccta aagagtggct
    19381 gtccatattc gacaactgac tcgttttcta caaggatttc tgtgatcact ccgtcggcct
    19441 cactagttat ctcattcatc agtttcatcg cttcaataat gcagataact tctcctttgg
    19501 caacttgatc acctactttt ttgaaagctg gtttgtcggg cgccggttgc agataaacga
    19561 ttcccacgat tggcgaaaca acctcgattc catcaaatac tgccgattct ttttctttca
    19621 ttgtttctgg agcagttgtt gcttgttcag gtatgacatt gactggctcc gaaactggca
    19681 acgtttcgtt tgtttgaacc atattcatgc ctcgacttgt tttattttta ttcatataca
    19741 attcaaattg accttcacgc aaatcgaatt ccgttagcga ggattgatca aattgactga
    19801 ctaattctct gatttcattg atatccaaaa ttattcctcc cagcgtttca tgcacagaac
    19861 cgcattatgt ccgccaaacc ctaatgaatt actcattgca taagtaaaac ttgcaggttt
    19921 gctttcgttt ttgacaactg tcaagtcaat ctcaggatct tgtttttcta cattgatcgt
    19981 aggtgggata aattgatgtt ctaatgccag caaagttgcc aaggcttcga tcccgccggc
    20041 tgctcccaat aaatgtccag tcatcgattt cgtgctgctg acgtaagtat ctctgaaatt
    20101 ttcacctaat gcgatttgga ttgcttttgc ttctgcctgg tcattggctg gtgtacttgt
    20161 tccgtgagca ttgatatatc cgacttgttc cggctgtaca ccagcttctt ccatcgcaag
    20221 cttcattgct ttagacgcac cagaaccatc tggagtagga gctgtcatat ggtaagcgtc
    20281 acagtttgca ccgtatccaa tgatttctcc atagattttg gcaccgcgag cttgtgcatg
    20341 ttctaaagat tcaagaacta gaacacctgc tccttcaccc atcacaaaac catttcgatt
    20401 ttcgtcaaac ggaatcgaac cacgattagg atcagttgat tctgagagtg ctgttaaagc
    20461 tgcaaatcca gcaatcccca tctcacaaat cgttgcttct gttcctccag caagaatgac
    20521 atcagaatag ccatgtttga tgttacggaa tgcttcccca attgaattcg ttccgctagc
    20581 acaagctgtt acgatagctg tacaaatacc ttttgcacct acgcgcaaag caatatttcc
    20641 agctgccatg ttggcaatcg ccattggtac gaacattgga gaaactcgtt ttgcaccttt
    20701 ttcatgtaga cgaataactt ggttttcgat tgttggaaga ccaccgatcc cagatccgat
    20761 gatgacgcca aaccggtctt gattcatttt ttctttatct aaaccgctca tctccattgc
    20821 ttctaaagca gcgtagattc catagatcga aaacatatcc attcgtttgc tatctttttt
    20881 aataaaatat ttatcgtaag ggaaatcttt gacttcaccc gctaaggtaa tccctgtttc
    20941 tgaagcatca aatttagtga tcggtgcgat gccattcgtc ccctctttta agctattcaa
    21001 aaagctgtct gcgtcatttc caataggtga agtgactcca tagccagtaa ttaccactcg
    21061 attcatttgt ttgctctcct tttcttatcc gtgcatgacc aatccgccat cgacattgat
    21121 gacttgtccg gtgatatatg gattttgtgc taagaaaact gctgtttgtg caatatcttc
    21181 tacttgtccg aaacgctgca tcggaatttg cttttccgct tgttccttga ctttgtctgc
    21241 taaaacttcc gtcatatcag ttgtgatgaa tcctggcgca atggcattac atgtaatgcc
    21301 tctagttgca gcttctcgtg ccacagattt tgttaatcct actacgccag ctttactagc
    21361 tgcataattt gcctgaccaa tatttccaat caacccagat acactagaga gattaatgat
    21421 ggccccttct cgctgcttca tcattttttt caatacgtgc tgcgtcatat tgaaggttcc
    21481 aatcaaattg atatcaagac attttttgaa atcttctgca tccattcgca tcacaagttt
    21541 gtcatttgtg attccggcat tgtttactaa cacatggatc gatccaagct tttcttctgc
    21601 ttctttgatc atttgtcccg ctttctcata atcagagatg tcaccggaaa ctcctacgca
    21661 ttttacaccg aatgcttcga tttcttctat cttttctttt ggaatctctc cgcgaccatt
    21721 caaaatgata tttgctccag cttttgcaaa agctaaggcc atcgctttgc cgattcctct
    21781 tgtactacct gtgataaata cattttttcc ttttacatcc ataacgttcc tccttatagt
    21841 attgctattg tttcatcaaa tgtctgctta ttctcaacac gcagaactgg tattgtttta
    21901 tcgatttttt tcataaatcc gcttaatact tttccggggc cgacttcgat gacttgttcg
    21961 atgccgattg tttttaactt atggatactc tcatagaaac gaacaggttg catgacttgc
    22021 tgttcaagta gtcccgcgat tgtttctttc ttcattattt ccgttgttgt gttgcttatg
    22081 ataggaaaag aaggttcaga gaattgaatc tgacttaggt ccttcgccag tttttttgct
    22141 gccggttcta aaatagctgt atgaaaaggc ccgctgacat ttagagggat cattcttttg
    22201 aacccttttt cttttaatag taaaacagct tcgtctactg ctttttcttc gccaccaatc
    22261 acgatctgct gaggtgtatt ataatttgca ggagatacga tgccgtactt gctagcttca
    22321 tgacagcttt cctcgatcgt ttcaatcggc gcattcatca ctgcaaccat tttcccgctt
    22381 ccagctgggg cagcttctgt catataagcg cctctttttg ctactaacgc aactgcttca
    22441 gtgaaggata gcgcattact agctactaat gccgaatatt ctcctaaact cagtcctaaa
    22501 gctgcatctg ggatgattcc atgctccttc aataagcgat aatacgcgat gctgactgtt
    22561 agaatagctg gctgtgtata ttgcgtctga tccaaacgtt cattttctgt aaagcaaagt
    22621 tcagccatat cgtagccgag aatctcactt gcttcatcaa atgtctgctt tacaacggct
    22681 tcttcatata aatcttttcc cataccttga tattgtgcac cttggccact aaataagaat
    22741 gctgttttca tgtttttctc caatatatcc agaatatatg tttgattctt cctattcaaa
    22801 aaggccgaga cattcctatt tcaggaaatg tcccagcccc caacctaatt ttaatgatta
    22861 tgcagtttgt ttttctacgt aagtcactaa atcttgaacc gttttgattc cgtcttctgt
    22921 ttcgattttc acgtcaaatt catcttcaat ttcattgatg atttggaata aatccaagct
    22981 gtcagcttct aagtcttctt ggatgtcagt agtcaatttg atttcttcag tttcttttcc
    23041 taattcttct gcgatgattt cttgaatttt ttcgaatacc ataataatat attcctccga
    23101 tattgtttgc tttttatttt tataatttaa cagtctacaa ctccatgaga atacttcccc
    23161 atgttaaacc tccgccatag cctgtaaata ttactttctg ctgttttccc aaggtgatct
    23221 ttccactaga aactgcttca tccaataaga tagggatgct tgcagcagat gtattcccat
    23281 atttatccat attggtcagg aacttttctt gcggcatctt tacttttcgg gcaattttct
    23341 caataattct gaggtttgcc tgatgcaaaa gcagataatc aactggtttg tctttcatga
    23401 cttcttggat attgtttgca acgtctcgta cggcaaaatc aaaaatatct ctgccagtca
    23461 tttgcaaata atacgaacct tctgaatctt ctgaatagaa cggattttta ttttgctggt
    23521 agccagcggt caatgagcta cttctggtac cgtcagctgc taatttttct ttcagcaatt
    23581 ttttctgtgg attcgcttct aagagaatcc cacctgctcc atcgccaaac aacacagcag
    23641 tcgtacgatc agaccaatcg attagcttag atagtgtttc accaccaatc actagtccct
    23701 tagttgagcc gcagcggatc attttttcag ccatgcttaa cgcataaaca aatcctgagc
    23761 acgcagcact gatatcaaaa gcgatagcat gagttgctcc caatttccct tgtacttgtg
    23821 ctgctacaga tggcgtcgtg taatcgggcg acattgttgc tactaaaata aaatccaaat
    23881 ctttagggtt tagatttttc tgttttatca atttttccgc cactaaaatg cataaatcag
    23941 aagtctcctg atttgttaca catcttcgtt cacgaatacc cgtccttgac gaaatccatt
    24001 catcagaggt atccatcatc ttggaaagat cattattagt aataatcttt tcaggaagga
    24061 cgctggctgt tgcagtaatc attccaaatt gttccattaa atatcctcac ttatagtctt
    24121 gcaagaagtc gtgcaagttt ttcagtgcat ccagcaaggc atgctgttcc tccgtagcca
    24181 ttccgtctaa gatgttcttg accatttcac gatgaaaatg ctgatggacc cgaaatagta
    24241 actttccttt tttcgttagt cccaacttta caacgcggcg gtcatcttca ctgcggatac
    24301 gttcgacata gccttttttt actaacttgt taatagcagt ggtcaaggtc ccaacagtaa
    24361 ttgatagttc tttggctact tctgaagttg tttttttctt gtacatcccg attgcctcga
    24421 tcgtgtgcat ctctgtgatt gacagatctt tgaattgaga ttttttcagc tcagactctt
    24481 cgattgtcaa aatatcattg aagacactaa caagataatc atttactgtt tttaaatctg
    24541 gttccatgtt ccaccgtcca ctaattagtt tgattatcaa atcttttgat gttcaaacta
    24601 tatcttaact tttttcaaaa tgcaacctta aaagatcatt tttttactat atgagctttt
    24661 tttaagattt tactcattta aaacaactga taaatcagca tttataattt cttttattag
    24721 aaaaatcatc tatctcacat attttgatta tcaaagtatt ttctgtgaac agaactatag
    24781 aattccttaa aacttgtcca ttatttcaaa atttgttata gtctttttat tcaaagcgta
    24841 aaaggagatc ctatgttaaa agaaaaaatg aaaactctcc ccatgctgcc gggtgtctat
    24901 cttatgaaag accgtgatgg taaggtcatc tatgttggta aagcaaaacg gctcaaaaat
    24961 agagtaagca gctattttca tcagaataaa cagcattcaa aaaaagtatt acgtatgatt
    25021 catcatataa cagattttga tttcgtcgta gtagacacgg aattagatgc tttattgctc
    25081 gagtgtcaat tgatccaaca ttaccgtcct ttttataacc gacaaatgaa ttatttcagt
    25141 aattataatt atgtacatat taccaataag ggatttgtat taacagatac tcctacggct
    25201 cgaacatacg ggccttttcg tctctataaa aaaatgccgt ctattttgcg tataatggaa
    25261 gaaacttatc aaatgccttg gttgtctgaa attagtttgt tagctttgcg agcccagctt
    25321 cctgacctac aggaaatgac ttttgaacag aaaaaaaaag gaactccaag ggctgtttca
    25381 aggccaaaat aaaaagctac taacttactt gaaaaaaaga caacaacatt tcatctatca
    25441 gttgaatttc gaaaaagccg gtatgcttca aaaagatatc gaattagtta cttattttat
    25501 tagacggatc caggaacaaa agcatttttt aagaacacca tcgctcacct tcagcatgcc
    25561 attagcagca gatgagagtc agaaaaagca ctacctcgtt tgttatggtc aacttgcaga
    25621 aacaatgatt gcctctgctg acgtttctcc tgacttctat tatgaaaaga aagaagctca
    25681 tttatctttg aaacgtcaat tgtcaaaaga agaaatcgat cctgtacaaa tcttgatcag
    25741 ttaccgcaaa aaattagaaa aagaacagtc tgaaattgaa ctgttaaaca aaaaagaagc
    25801 tgaaaagcaa ctaaattagt tgcttctcgg cttcttttag gcatcttcaa cttcaaatgt
    25861 caattatcct aaaatttatt ggattaagtc atagatttcc atcgctacga tatcgatatt
    25921 atcaaattcg tattgctgat gagtatctgt ttcagttaat tcaaatgtat cggtcgcttt
    25981 atcataagtt actgtacact tctctacgcc ttctttttca aaacggcgaa cttgcagttc
    26041 attatctgct gactcgacca tagcctccag tcgttttatg attgcgacta attgtgaatg
    26101 tgtcatcgga ggtcctcctt ttcttatttc tgttatattg tatcaaaagc atagcctact
    26161 tgcattgatt ttctctaaaa aaactcgttt tttatgattt tttttctggt atttttcctt
    26221 caggccacca aagtttgatc aaagcttggg ttccgtcatt ctcaaaacct ttatctgcca
    26281 actcttcata tagtttcaat gcttgggtag ttgctggcaa tacaaggtcc attttttctg
    26341 cttcttctaa tgcaatcttt aaatctttga taaaatgttt gacaaaaaaa ccaggtgtgt
    26401 aatcttcttt taaaatacga ggagaataat tgcttaaaga ccagttagct gcacttccac
    26461 ctgaaagcgt ctcaatcact ttctttaaat caagtccgtt tttttgtgca tagacaagca
    26521 tttccgtcat tcctgtcatc gtacctgcaa tcatgatctg attagccatt tttgtatgct
    26581 gacctttccc agcagaacca tgcaatgtaa atgtcgtacc aaattctttg aataaaggca
    26641 gtacttgatc ataaacggct tcttctccac cgaccatgat cgtcagtgta ccattctttg
    26701 ctcctaagtc accaccggat accggtgcat ctaacgcatc tgcgcctttt tcttttgctg
    26761 tttttgcaat tttttctgcc aggcttggtg tacttgtagt caagtcaact aaaatatgtc
    26821 ctgagatatc tgcagaaaaa atccccgact gtccatagta gatttcttct acatctttgg
    26881 ggtaacctac cattgtaaag agaatattac tttgttctgc taccgctttt ggtgtatctg
    26941 cccagatagc tccttctgtt actaaaggat cagcttttgc ttttgtccga ttatagacag
    27001 ctacttcgtg tcctgcttct agtagatggc gtgcgacagc acttcccatg acgcctgttc
    27061 cgataaaacc aagtttcatt ttttcgacct cctaaaaatc aaaaatcctt gcttctatgc
    27121 tagtatacgc tagaaacaag gattttttca taaaaaatgt ttgaatttta tcttttaaat
    27181 gaatttaatt ttattttgat tcgttcaatc gagtcttctt cttcttttct tcgcatctct
    27241 tcttcatgat aaacaaccat gtcttgataa aattcgatca tagcttcgcc gaaatgtgta
    27301 gatgaaatat cataaagttt agcttctaaa acttttggat ctttaggaac ttcactggaa
    27361 atataatcaa taagtgctgg tgcaaagtct tcgtcggttt caaaagtttt ccctaatgaa
    27421 ggatgatcaa acaatcggtt caaataagca tttccttcca cgacacaagg tgttccggct
    27481 gccatcgctt ctgtataagt aagcccttgt gtttctgaag tagaagcact gacaaaataa
    27541 tctgccgctt tataataaag tgctacatca ttgttgtcca cttctcctgt gaactgcaca
    27601 aattcttcta actgcagttc tgagatcaat tcctctaact cttctacata aggtcctttt
    27661 cctacgataa ctaatctggc gttaggataa gcggagatga cctgaggaaa cccattaatc
    27721 aaggcttgga tatttttttc ataagaaatc ctgcttaaag aaagaatcat caaatggttt
    27781 tcctgaagac ctagtgactc ccttaatttg ctgatatcct gctgagtgat atcagaacgt
    27841 ttgaattttt caatgtcgat accagtagga atcacgcgaa gcggtgctat tacaccatag
    27901 ctacgtaatg tatcaataac acgttcactt ggacaaacta cacctgtcgt atgatttgca
    27961 aataatctag acaagtattt tacatgggat tgcctgatca cttttccttt accgatataa
    28021 tgaaggtagt cttcatacat cgtatggtaa gtatggatca gaggtatttt caacttctta
    28081 gctaccatct tcccaagtat ccctgctccg aattctgtat gggtatgaat aagatctagt
    28141 tctagttctt tggcaaccaa ataagcatac cacatgcctc taacaacaac tcttcgatcc
    28201 ttgaaagaaa taaatggtac acttggcatt cgaatcacat ctttttccaa cttatccgca
    28261 ttaggatcgg tcgttgtaaa aatatagact tcatgcccat gtctttctaa ttcttgtttt
    28321 aatgttttga tcgatgtagc aactccgctg acttgcggaa agtaggtatc ggtaaaaaag
    28381 ccgattttca tgtttttcgc ctccaaacta gtccttgacc tgatttactt tttttaattt
    28441 gctagagact ttttcattca gtacactttc gtatacattt ttaagttctt caccgatttt
    28501 attgatactt cgctcttttg ctgtttgata accggcttgt gtcgtactcg gtagttcttg
    28561 attggtaatc tgttggatca gtcggataaa ttcttcgttt gtcttcccca tatagcagtt
    28621 cttgctatct tctaaccagc cattatacac tggaatatct cgaacaagta cgttttgttt
    28681 gcttgctaaa gcttctaaaa caacgatccc ttctgtctct tcataagaag ggaaaaagaa
    28741 tagatctgca cctgaatacg caccttctat gatctctcca cgaatgtaac caggaaacag
    28801 gacattttca ggatgatctt cttttacaat tttccggatg ttccgaggaa tcgagtacat
    28861 gggaacatga ccaaaccaaa taaatgtgta ttcgggcatc ttcttagcaa tttctacgaa
    28921 atcaatgatt cctttacgtt cgaaaaacaa accgacacaa atgattactt tttgatccgg
    28981 agacagtttg aaatattctc tgaacttttg ttctttcatt ggatcagggc gatatttctc
    29041 tacgtcgatc ccattggaaa tagcttgaat cggcacacgg atactatagc tttccaacaa
    29101 ctgttttgaa taaggtgttg gcgtgatcag atagtccgct ttttggtaca atcctaccag
    29161 atattttttt acgataggag aaagctggtt cgatccgata aaggaatttc ggaaatcttc
    29221 ctctgtagaa tgcgcatgat agacaacttt ttttccttct cttctggctt tattgaccat
    29281 attatgacta ttgattccgt atgtattgat gtgcaggatg tcataatctt ctttttcatc
    29341 tagtgtataa gagataccga cttccgtcag agcacgtttt tgatgatcaa gcgcacgccc
    29401 gattccagat tttgcaagca tcttttcact ttcaaaatat aataatacct tcaccttacg
    29461 tcccccttca cttatatagt ataacatagc ctacaaactg tcactcaaac ttaagcccta
    29521 tttttatcta ttcttactga caaaaacgtt ttcttaaaaa agaacaaaaa aactggcgta
    29581 gctcttttac aagccacacc agcttttgat acgttgttta ttttacatat tcatgaacaa
    29641 gttcaacgac ttcttccatc gtatcgcatt ctttcaatgc acgatcagca agttcagcca
    29701 ttttcgctgt gtccaagcgt ttcattaaac tacgcgtttt aagaatagat gtcgcactca
    29761 ttgagaactc atctagaccc atacctacta gcaatggaac agctgtttga tctccagcca
    29821 tttctccaca catacctgcc cattttcctt cagcgtgtgc tgcatcaatt acatttttga
    29881 tcaaacgtaa aattgatggg ttatatggtt ggtacaagta agaaacgcgt tcgttcatac
    29941 ggtctgctgc cattgtgtat tgaatcaaat cgttcgtacc tacagagaag aaatctactt
    30001 cttttgcaaa cttgtctgct aagactgctg ctgctggaat ctcgatcatg atgccgactt
    30061 ggatcgtatc agatacttcc ttgccttcac tgatcaattt ttgtttttct tcttcaaaga
    30121 tcgcttttgc tgcgcggaat tctttgagtg tagctaccat agggaacatg atacgtaagt
    30181 taccatgaac agatgcacga agcaatgcac gcatttgtgt acggaacatt tcgtcgcctt
    30241 gttcagataa actgatacgc aatgcacgat atcctaagaa tggattcatt tcatgaggta
    30301 attgcagata tggtagttct ttatctccac cgatgtccat tgtacgaaca actacaggtt
    30361 ttccttccat accttctaat acagctttgt aagcttcata ttggtcatct tctgttggga
    30421 agtctggaga atccatatac aggaattcag tacggtacag accaactgct tctccaccat
    30481 tattatggac accaactaga tcttttggtg ttccgatgtt tgcagctaat tcaaaatgct
    30541 tgccgtcagc tgtgacagtt tctgcatttt ttaatttttc ccattctgct ttttgtgctg
    30601 cgtaatctgc acctgctttt tcaaattcag ctttttgttc atctgtagga tcgatgatta
    30661 catcgccttc gatcccgttg acagccaaaa tatcgccttc tttgacttta gcagtaattt
    30721 cttttgtacc aacgattgct ggaatttcta aagaacgtgc catgatagca gaatgtgaag
    30781 tacgtccacc aatgtctgtt acaaacgctt ttacaaaatt gcgatctaat tgtgctgtat
    30841 cacttggagt caagtcatgt gcaacaacga ccacttcttc gttgatcatt gatggatttg
    30901 gtaaagtaac gcctaataag tgcgctaaaa tacgtttagc aacatcacga atatctgcag
    30961 cacgctcttg catataagca ttatcttcca ttgcttcgaa cataccgata tacatatccg
    31021 ttacttcttt tagcgctgat tcagcattta cgctgttgtc tttaatattt tgcttgattt
    31081 gacctaccat ttctggatct gaaagaacca tcaaatgggc atcaaatact tgcgcttctg
    31141 cttcacctaa gctttgcgct gctttttcac gaatttgctg caattcttca gtagatttag
    31201 ctaatgcagc atccaaacga gcttcttcag ccgcagtatc ttctactgtt gccttactga
    31261 atgttaaatc cggttgaact agcaggtaag cttttgcaac ggctacaccg tcactagcgg
    31321 caatcccttt tagcatttca accattattc agataagcct tctttcttca ttgtttcaac
    31381 gattgctgcc attgcatcag cttcgtcagc accttctgca gtaatagtaa catcagaacc
    31441 ttgacctaca cctaaagaca taacgcccat gattgatttc aagtttactg atttaccttt
    31501 atactctaag tttacatcag agttaaattt gcttgctgtt tgtactaata gtgtagctgg
    31561 acgagcgtgg atcccagttt ctgctactac gtgaaattct ttcttttcca tattaatcgg
    31621 tctcctttaa gtgagtgttg tttttatgtg agggttttct tttttagtaa ttcagagaat
    31681 cctaacaaat gccacccttt catctgtaaa gattaacatt ttttttcgtt agatacaaca
    31741 gttttcattc aatttcagat aaaagtattc aaattattcc ggattctttt tcatcctttt
    31801 cttcacattt tataacaaaa gtgtttattt tctttttctt gtcgctttct cttctcctga
    31861 cgtttatcat tctttccttt gaccctcttt gttttcatat gaaacgaatt acttgcttcc
    31921 ctttttattt ttagtataat aagagtcaaa ggtcaggaat ggtcaataga gatttcacct
    31981 tttctacctc ataaatctct taaaaaacta ggggttattc ttttacctca tgaaaaacga
    32041 gatgtaaaat atggatgtac ccatttttgt gaaaggacgt gaatgattca tgctttgtca
    32101 aaactgcggc aagaatgagg caacgattca cttgtatgcc actgtcaatg gtcagcggac
    32161 acaactcgat tactgtcaga gctgttatca aaaaatcaaa aatcaacaaa atggaggtct
    32221 gactaatatg gcccaaaatg atccatttgg atttggcagt ttggacgatc ttttccgttc
    32281 catgtctcgc caaatgcaac agcaaggaaa ttatgagcaa acaccaccaa cacaatttgg
    32341 cggaaacaac aacttcaatg gtggtcagcc acctcaaggc aatacagaag gtttattggg
    32401 agaatacgga atcaatatca cagaacaagc acgacaaggt gatatcgatc ctgtgatcgg
    32461 acgcgatgac gaaatcaaac gagtcattga aattttaaat cgccgaacca agaacaatcc
    32521 tgtcttgatt ggagaaccag gtgttggtaa aacagcagtt gttgaaggtt tagcacaaaa
    32581 aatcgtagat ggtgatgtac cgcaaaaatt aatggacaaa gaagtcattc gtttagatgt
    32641 cgtatcttta gtccaaggta ctggtatacg aggacaattt gaagaacgta tgcaaaaatt
    32701 gattgaagag atccgtcaag cagaaaatgt gatcctattc atagatgaag ttcacgaaat
    32761 cgtcggtgct ggttctgctg gtgatggaaa tatggatgct ggtaatattt taaaaccagc
    32821 tttagctcga ggagaattgc aaatggttgg tgctactacc ttaaatgagt accgcatcat
    32881 cgaaaaagac gctgctttag aacgtcgtat gcaacctgta cgggtagatg aaccaactgt
    32941 cgaagaaacg atcagtattt tgaaaggttt gcaaaaacgt tatgaagact atcatcatgt
    33001 aaaatatact gatgaggcca tcgaagcagc cgcaacttta tccaatcgtt acgtccaaga
    33061 tcgcttcttg ccggataaag ccatcgattt gctagatgaa acaggttcta agaaaaactt
    33121 gacgatccaa atcgttgatc ctaaaacaat cgagaaaaaa ctgcaagaag cagaagaaca
    33181 aaaagtttta gcttctcgag aagaagattt cgaaaaagca gcttattatc gcgatcagat
    33241 caataaactt caaaaaatga aagaacgtca gctcactgaa gaggaaacac ctgtgattac
    33301 cgaaaaagat atggaaaaaa tcgttgaaca acggacaggt attccagtgg gtgaattaaa
    33361 agaaaaagaa cagacacaac tgaaaaactt agcagatgac ttgaaagctc atgtcatcgg
    33421 acaagataac gctgtagatc gagtagcgaa agcgattcgt cgaaaccgtg ttggattaaa
    33481 caaacagaat cgtccaatcg gctccttctt atttgttgga ccaactggtg taggtaaaac
    33541 agagcttgcg aaacaacttg cctacgaact attcggttca caagattcaa tgattcgttt
    33601 cgatatgtct gaatatatgg aaaaacatag tgtctctaaa ttgattggtt ctcctccagg
    33661 ttatgtcgga tacgaagaag ctggtcaatt aacagaaaaa gtacggagaa atccttacag
    33721 tcttgtcttg ttagatgaag tagaaaaagc tcatccagat gttttgcata tgttcttgca
    33781 aatcttagat gatggtcgat tgacagatgc acaaggaaga acggtcagct ttaaagatac
    33841 gatcatcatt atgacaagta atgccggaac tggtaaagta gaagcaaacg ttggttttgg
    33901 tgcggcacgc gaaggtgtta ctcgttctgt attgaaccaa ttaaacaact actttacacc
    33961 agaatttctg aaccgttttg acgggatcat cgaattcagt gcattatcaa aagaaaactt
    34021 gatgacaatc gtcacactaa tgctagacga tgtcaaccaa atgctggcag cacagcaact
    34081 tcacatcgaa gttcctacga atgtcaaaga aaaattagtt gacttaggct atgatccatc
    34141 tatgggtgct cgtcctcttc gacgcacgat ccaagaacaa atcgaagatg gtatcgctga
    34201 attttatctt gatcaccctt ctatccatga gttgaaagca aaactggata aagatggaaa
    34261 aatcatcgtt acttctaaac cagaacgttt ggctaaagaa tcagccgaag aaacggctga
    34321 ataaatccag ttttatagcg tgagacggta tttctttgag aaatgccgtc tcattctttt
    34381 ttcagaaaag tcaaaatact gttcatttta taaaaaagaa attataataa gagaaacaag
    34441 caattaaaaa tacataaaag aggtgagcgc catgaaattg gtaaatgtaa caaacagcca
    34501 ttcccgtctt gtattaaacc agcttgaaaa cactgatgcc catctagtca aggtctacac
    34561 agccggaaat accactattg tctacactga agcccctgaa cataatgaaa ttctgctgat
    34621 caatgacaaa cgtaaaatcc agccgaaaga aatcgaagat gctaaaaatt atttcttaga
    34681 taaaatcaaa gacgccgttt accacgagga tgaaatcaaa gtaatcgaat tggcaggcct
    34741 tgttgaaatc tcgattccaa aacatgaatt aggcgctaca ttagccgtgt aaacaaattt
    34801 tgaacataat aattaaagag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta
    34861 aacacgtata aacggcggaa gcagaagcaa ctccttcgga aataagctaa aattcacgaa
    34921 aattgctttt caaatttttg tgaattccag cttatttctt ggagttaaac gcttctgtct
    34981 cagcctcttt ttctttctag ctttttgatt tttctctttt cttttgtact tttctctttt
    35041 cattttcagt tctacgcgtg tctttatttt taactttttc ctctttatgg atgattgatc
    35101 gtactttttg tgcaatttgc tcgtttttgt cccagtcttt tacatctaat aatcgacata
    35161 atcgtaacat ctcttctttt ttctgttttt ttctttcacg tatttgccat tctgtttcca
    35221 tgtgtcacac tctcctttct ttaatcatat gtacgcaaaa taatttttat atttttttga
    35281 ttagacattt ctatgatttt aatatagtaa aaagagccgt cacggaaaat ccgtcacggc
    35341 tctaaacaaa atatgagagg ctgtttttgt ttggctattc ttattacttg atgcagggca
    35401 gcttgtgtca agacttcttt acatcaaact ttttaatttt acattaggat atttatccgc
    35461 aaaccagcgt tctgcaaatt ggttctcgaa taagaacaat ggttggtcga aacggtcacg
    35521 agccaagata ttgcgacttg aactcatctt ctcatccaaa tcttctggat cgatccagcg
    35581 agcgattttg tgtcccattg gagacatgat tacttcggcg ttatactcat ttaacatacg
    35641 atattggaat acttcgaatt gtagctgacc aacagctcca atgatatatt cttccgttag
    35701 atatgtttta taaagctgaa tcgctccttc ttgaactaat tgatagattc ctttgtggaa
    35761 ggatttttgt ttcatgacat tttttgcagt cactttcata aacagctctg gagtaaatga
    35821 agggagttct tcataagcaa cttttagttt tccttcgaac aatgtatccc caatctgata
    35881 attgcctgta tcatatacac cgatgatatc gcctgctact gctttttcga cgttttctct
    35941 cgaatctgcc ataaattgtg tgacattgct taatttcagt tttttatttg tacgttccaa
    36001 ccagacatcc attcctcgtt cgaatgttcc agaacagatt cggacaaaag cgattcggtc
    36061 acgatgggct ggattcatat ttgcttggat cttgaatacg aaacctgaaa attcttcctc
    36121 ataaggactt accatctgat catcttgtgt tttgtgtcca taaggagctg gtgccaattc
    36181 aacgaaagtc tctaaaaaag tctgtacacc gaagttagtc aatgcagaac caaaaaatac
    36241 tggtgtctga tcgccacgag caattttttc ttgattaaat gcatctccag cctctttgat
    36301 tagttctact tcttctaaga cttgttcata taaggaattt tcagttagag gatgattttc
    36361 gggaagcagt ccatcttcat ccaattctac taatcgttca tcctgataat tttccggacg
    36421 ataaaactct acacgattat ggtagatatc ataaagacct tctagccctt tacccatacc
    36481 gattggccaa ttcatcggat aagattcgat atctaatagc tcttctaatt cttctaacaa
    36541 ttccaatggt tcacgaccat cacgatctag tttgttgata aaagtaaata tcgggatgcc
    36601 acgttttttt acaacttgga ataatttctt tgtctgtgct tcgatccctt ttgcactatc
    36661 gattaccatg accgcactat ccacggccat caaagtacga taagtatctt cagaaaaatc
    36721 ttcatgtcct ggtgtatcca agatatttac tcgtttccct tgataatcaa actgcataac
    36781 agaactagta acagaaatcc cacgttgttt ctcgatttcc atccagtctg atttggcaaa
    36841 attacctgtt tttttgcctt ttacggtccc ggcttgacgg atagccccac caaacaatag
    36901 taattgttct gtaatcgttg ttttcccagc atccggatgg gaaatgatcg caaaagttct
    36961 gcgattgtcc acttgttgct ttaattctgg attattcata acctaactcc atttcttcca
    37021 ttcaattatt tatattttct ctaggcacag attgtccgat ctcttaaaga aagacaattt
    37081 catatagggc tgtgacaaaa gccgtgtagc agtctctttt tccgagtaaa cggcgttcta
    37141 gagacagcct atcagcaata agcccaccca agccaaaaat acgaaaagct gtttttgctt
    37201 ggctgctctt attacttaat gcaggatagc tttttcacaa cctttgtcaa ctactacaac
    37261 tgaatcagta tagcatggat tcatcgaatg aaaaagccac caaaccataa gagtcaaata
    37321 tttttttgtc agctgactaa aagaaaagac gtgttgcttg tttattcaag caacacgtct
    37381 ttcataacca agtttccaat tatcttcttg gctcgtcatc ttcgaattct tttctgaagt
    37441 atcttcgttc ttcttcaggt tccgcttctg tttcttccgg atcatgaaaa atgacacgga
    37501 ttttaaggat tcgagaacct tccatttctt cagaaaccag cgtaatatta tcgacatcaa
    37561 acgataactt ttcgccttca tccggaatca tgcctaatgc tgtaatcaaa taaccagcca
    37621 tcgtatctac atcactcata tgcagatcac tgtcaaaagc ttcattgaac tcatcaatca
    37681 gcatacgtcc ttggatgata tattcgtgtt catcgatttt ttcataaaga ttctcaactt
    37741 catctgattc atcatcgatt tctccgacga tttcttctaa aaggtcttct agtgttgcta
    37801 acccaacgac gccgccatat tcatctaata aaatagccat ctgattctgc gtctttttca
    37861 tttcatataa cagatcatca ataaagaccg tttcaggaac gaataaaggc tcctgcatga
    37921 ttttcttgat atctaaatta tcaaaaccga atttatgggc agcttttagt aaattctttg
    37981 tatgaaggat tccgactact ttatctttgt cttcgttata aacagggatt cttgaatagt
    38041 tttcggataa aacttcattc acattttctt ccacagtgtc gttgatatca atcataaatg
    38101 catccgttcg tgggaccatg acttctcggg caactttcgt atcaagagaa aaaacccctt
    38161 ggagcatttc taattcttca ttattcaata cgccttcgtt ttcaagcata taacgcattt
    38221 catctctggt catttttgaa tcttcatcat caaaagtcat cggcgtgata cgcgccaata
    38281 aacttgtgga agcagaaagc agccagacga agggcttagc tactacacca atcaagcgta
    38341 tcagccctga agtgaacttt gcgacttctt ctgatttatt taaagcaata cgtttagggt
    38401 acaattctcc aaaaacaatc gatacatagg tcaaaatagc cagcacaatg atatttgcaa
    38461 tattttttgc cgctgtgccg ccacctaata ccggagctaa ccgttccgat aaagtattag
    38521 ctagtgaagc accagacaaa atgttgacca aagtgattcc tacttggatt gtcgatagga
    38581 aattatttgg attctgcaat atttttaaaa gcttttgaga tttcacatct ccttcatcag
    38641 ctttttgctc gattcgattc ttattgatcg aaacaacagc aatttcggaa gctgctaaaa
    38701 aagcattgat caatgtcaaa acgatcaata ataaaatctg cgctaacagc gactgactct
    38761 cagggtcagc attcataatc gcattctctc cttataaaat taatttttag tttacgatag
    38821 aaaaaaacgt ggcactatag ccacgttaaa atggtatcac aaagcaattt tctttgcaat
    38881 aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa ttttcttctt gttttcattt
    38941 tgcttgtctt ctttgacgat tttgattata taggaaatga tcgtcttgca gattgcgtaa
    39001 aaaggaacac ctaagaagat accaagcaat ccggcaatat ttccagctac tagaagaacg
    39061 ataatgatcg ttaatggatg gattttcagt gatttcccaa ttacattagg ataaatgata
    39121 ttcccatcca actgttggac gattagcaca accacacaac aaagtaatgc ttttaccgga
    39181 tcattgaaga ctgttactag aaatgcaggt gctagtccta gataaggtcc taaatatgga
    39241 attaggtttg tcaatcccgc aatgacccca aataagaaag cataatccac accaattacc
    39301 atatatccta aaaatgtgaa ggtaccaacg aataaacatt cgattgcttg tccgctaatg
    39361 taattagcca atgttttatt cagttgcccc aataactcca caatttggtt tcttcttttt
    39421 tctgggaaaa actgttggat gtttggcact agtttttctc cgtctttcaa catatagaac
    39481 aagatgaatg gtgctgtaat gattactacg gttgccgaag cgatcgttcc aacgatcgaa
    39541 cctaaactat tggatactcc actaaggaat tgctgaatga ttgtcccgta agaaatgtct
    39601 aattgctcaa aatatttatt gatatctact tccttgaaca aaggcaattc ggctacctct
    39661 tttaaccagc tttctacctg tttgatgaaa tcaggcatat tagatgccaa tgaggaaatt
    39721 tgctgtacta gacttgggat gacactaagt aaaatccaaa taattgctgc aactaaaagg
    39781 agcagcacaa ttgcgacagc ccatattctt ttgatttttg ctttcatcaa cagcttgaca
    39841 ataggattca ataaatagta aagaaagcct gcaatcagta caggtgcaaa taacgtggaa
    39901 aaaaatgtcc cgatcggttc gaaaataaag tttatttctg atgaaatcat gatcaatgca
    39961 gctatgatca ataattcaag cgaccaaaac attagtttag actgtctaag tttttcaaac
    40021 aatagatttc ctcctttctt tcttactagt atatattata ttcgttaaaa attcttttat
    40081 tcttttttta tttttgaaaa cttgccatac tttttatttg ttttattttc cagtatacta
    40141 tacgaaagga aggtggctag accatgaaag ttgtacaaac aaaagataca atgagcaata
    40201 tttacttaga tgctgtacgg atcaggcatc aagtttttgt agtcgaacag ggtatgcctt
    40261 tgtctcgaga aatagacaaa gatgaagccc attgtatcca cttcgtcctg tactcagata
    40321 aaaaagaacc gcaaggaact gtacgtcttt tgcctttaga aaacggaaaa atgaaattac
    40381 agcggatggc gatccttttg aagatgctgg catgcctcat atttatatga aaaagacttt
    40441 ataaaaattt atttcgaaaa aagcaaaaaa cagcaagctt ttgagaaaaa tctctcactg
    40501 gcttactgtt cttttttgtg tttacttacg cgacaattgc ttgtaacggt tataccaaat
    40561 atcaatatag ccttccgaga acgggccatc tccattattg atccagtcaa ccagaatctt
    40621 cacattttct tttaggatga agtcaatatc gttcggataa ttcatcattt tgctgtgcat
    40681 ctcatattca tcgacatcca atagtcgttt ctcgccgtca ggaaatacct tgatatccaa
    40741 gtcatagtcg atatatttca aggcctcatc gtccagcaaa aaaggtgaag ctagattaca
    40801 ataataggaa accccctttt ctctgatcat tgctattaca ttgaaccagt attttttatg
    40861 aaaataaaca atcgctggtt cgcgcgttac ccatcggcgt ccatcagatt cagtgactaa
    40921 tgtgtgatca ttcacgccga tcatagagtg ttcgcttgtt tttaatacca tggtatctcg
    40981 ccacgttcga tgcaatttgc cgtcgtgctt gtaactttgg atcgtcacaa actctccttc
    41041 ttttggaact cccatagcaa cccttcctta agcagcattt tgtgctttgt gtttgggtgt
    41101 tcattacacc gacatcataa ttattatatc aataaaaatg agctttgcct agaaaggatt
    41161 cctattgttt cgatttttct ctaactgatc taccagtttc atttggacct taggaaaaac
    41221 gaccgaatca aatgcatcag gcttcaacca cttcgaagtt ttgttctcgg taaattcttg
    41281 ttctgctccc tcggttgccc gtccgtaaaa aaggagaaca tgccatttca gatgactaaa
    41341 aatatgagta acttctccta aatgtcgtgt ctgccaaaca aagagttcct gcttttcaaa
    41401 tgggagattt tgctcttctt ctgctactag gtcatcaaac agatcgattt ctggctttgt
    41461 ttcccattct tttttcaatc gctcatactc ctcttgtgtc acttccatca ttggaaatgt
    41521 ccacatattc gccagcaatt tttggctatc tctttcttca aaataatacg ctccagaatg
    41581 attttgaaga gccgcactga tataataaac gtcttttggc tttgcttttt ttgtttttac
    41641 aggaaaactc gtttggattc ctcgtttgtt agctaaacaa aaggcttgga tcggacaagt
    41701 ctcacacttt ggagaagttg gtgtacagat agctgaacct agatccatca ttgcttggtt
    41761 gaattcacct ggatgctttt catcaatgat tttccgcatc gcttcatcaa agattttcct
    41821 gctagaagct tttgcgatgt ctgcttcaat gcaaaacaat ctgctgacta ctcgcatcac
    41881 gttgccgtca accgcgggtt ctggaagtcc aaatgcgata cttgcaatcg cgcctgtcgt
    41941 atatggtcct attcccttca atgagctaat ttcttcaggc gtttgcggca tttccccatc
    42001 aaattctgac atgatctgtt tagcagcagc ttggatgttc cgggctcttg agtaataacc
    42061 aaggccctcc catgctttta aaagcttttc ttctggagca tttgctagct cttcaattgt
    42121 aggaaaccat tccatgaaac gataaaaata atcgatcact gtgtctactc tggtttgctg
    42181 aagcatgatc tcagagatcc agatacgata aggatcacga ttatagcgcc aaggcaggtt
    42241 tcttttttct tgttcatacc attgaatgaa ctgatcttga aattcttttg tttctttatc
    42301 tgtccatttt tcccattctt tattcacttt cctcttcctt ttccgtaatg aatcgctgga
    42361 tcatatccaa atcaaatcct ttttgatata agctttgctt tacttttaga tttctttttt
    42421 taggttcaaa ccttaaattt cttcgccata gtttatctcc ttgtttgact aaataatcat
    42481 attctatttc ttgatctgct tcttgcggaa gatcagccat cgcttgttgg atcacttctt
    42541 gagaaaaacc tttcgtcatc aatgtttgtt ggatttttct caataattct ttttggcttt
    42601 ttccgcgatt ttttccaaac gctttttcgg ctgtctgatg tgcattttga acttggtctt
    42661 caaatgtata ttggctgagc gcttgttcaa tgatttcagg tttcaaacct ttttgctgca
    42721 tctgctgcgc taatttcttt ggtcctttgt cacttaagcg catattcgtg cgcacataac
    42781 tttccccata gaccaaatca tcgacaaggt ccaattcttt taagcgggct acgattttat
    42841 ttcgatcttc ttgaaggatt tccttttctt ttaagtatgt tcggatttct ttttccgatc
    42901 gcaactgata acttaagtaa ttcattgcta ggtgaaaacc gaaatcagct ccgctactct
    42961 tcttgatctc ttggatagtt tcttcgtcta attcccgtcc ttttaataat cgatagcgta
    43021 ctaatacatc ttcagatact tgtaaagctt ctccttcaga taaatctacg cgatagaaag
    43081 ggcctttgcc tttactgatt ctcgtaacgg ttaacataaa catcctcctc gaacatatat
    43141 tctctatcat accacagaaa atccccgtaa tcaggtatta tatgttagaa taaaccacag
    43201 aaaaaagaaa aagggagata cttatggcag aaacaattgt caaaactgga caaacggttc
    43261 ctttgaaaat caagcgttta ggcataaatg gtgaaggaat cggttatttc aaacggttga
    43321 tcatatttgt tccatatgca ctaccaaaag aagaagtact agtgaaaatc acgaaagcaa
    43381 ctcctcgcta tgcagaagga gaattaataa aagtcaaaaa aacaagcaaa gatcgcgtag
    43441 tggcaccatg tcctgtttac tatgaatgcg gtggctgtca attacaacac ttagcttatc
    43501 aagctcaatt ggattttaaa aaggatctgc ttttgcaagc attggaaaaa ttcaaaccag
    43561 caggctttcg atcttatcaa cttttgccga cgatcgggat ggatgagcct tggcattata
    43621 gaaacaaagc acaatttcag ttacgaaaaa ataagcagac tcaaaaaatt gaagctggtt
    43681 tatacgaagc aaactctcat cagttagtcc ctataacaga ctgcttggtt caagaacctc
    43741 gaacacaaaa agtaatgaat actgttgttc aactattgaa taaatttgat gcacccatct
    43801 atgatgaacg gacaaacagt ggaatcttcc gcacaatcat ggttagaatc ggtgttaaaa
    43861 ctggggagtt acaagttgtt tttattactc gcagtaaaaa attcccgcaa aaaaacgcaa
    43921 tgatctgtga gatcaatgaa cgactgcccg aagttgtttc aatcatgcaa aatgtccagg
    43981 cacaaaaaac gtctctcgtg atgggggatg aaacgatcca cttatgggga aaagaagcca
    44041 ttgaagaaca aatcaatgaa gtgactttcg atctttctcc tcgtgccttc tttcaactca
    44101 atcctcagca gaccgaattg ctttatggcg aaggaatcaa agcattagat gtccagccta
    44161 atgaaacgat cgtggatgct tattgcggag ttgggacgat cggcttaagt gtagcaaaac
    44221 aagccaaaga agtacgggga atggataccg ttcctcaagc aatcgctgat gcaaaaaaaa
    44281 atgcggaaag attaggatat cacaacactc gatatgaagt cgggacagca gagcaagttc
    44341 tgcctaaatg gttgaatgaa ggttttcagc ctgatggtat catcgtagat ccaccacgta
    44401 cgggtttgga taacgcattg atccaatcat tattgaaata ccctccaaaa aaacttgttt
    44461 atatctcctg taatgtttct acacttgcca gagatctggt taatttaagt aaaaagttca
    44521 agattgaata tttacagtct gtcgacatgt ttcctcagac tgcacgttgt gaagttgtcg
    44581 tgaagatgac gagactcgct gactaaatga aagaggttgt gaaaaagtct gccctgtatc
    44641 aagtaataag aacagccaaa caaaaatagc tcttcatgtt ttatgaatac cgtacttgtg
    44701 accgaagagt taacaggagg acactattta tttaaaaaaa gggttgtgac atagtttttg
    44761 tcacagccct ttttgattat tattggatat tcacgtctat cacttgataa aatgcatttg
    44821 tcgtatcgct gatattccat actgctaaaa tcacattata tccttttcga tcctgcggaa
    44881 gtgtgatcgt ttggttgact gtttttggtg gaacagttgc tttgtcgtct atttttgtga
    44941 tcaattcaaa atcactaaat ttcaaaggtt gattaggatt ccaagttggt ttggtaatgt
    45001 agtaatccca agtacttgtc cgatgttgtg ctgttaaatt ccaagtaata tttaatgttc
    45061 ctggattaac catgttttta tgccatctgt tagatgtctg ttcatctaaa ggctcaaagc
    45121 cgctgactcc tgcactagca atttttccat caatgaatgt atttttggta gcttcgatac
    45181 tttgcggttc ccattgtgct cttccgacat tttgatttaa gtttcctgct gctgatgttc
    45241 ctaggtatgc acggcttcct ggagaagtaa tataaccatg cgcaaaacct ggaatgctaa
    45301 aaccgaacaa ccctaaacta actaacccaa taaacaataa cgtactaatc tttttcatgt
    45361 agaaacctcc ttcatttgat aaccacattg taaacgcttt attgtattaa cacaaacatt
    45421 atgcttttat aattttatgt tgaagtaaaa aaggtgaaaa aagtaagatg cagattataa
    45481 cagaagtaga cgagaaaagt acaaatagaa aacatgagaa ccaaatcata tgatcttttt
    45541 caaaaacatc attcatataa acaaatgttc aactttttaa taacaacaat catcaatttt
    45601 tttcttactt cacaaactaa atacaggtct tgctgtgagc cttcatggta aactactatt
    45661 atagaaagga gttagtaaaa tgaagatgat aaaagatatt cgtttttggg tttgtgttat
    45721 aggaattgtc atattaggct ttttatcagg gttgcttagc ggaaatcccg gagaatatta
    45781 ctattctttg caacttccac catttgcacc acctagctgg atatttgggc cgatgtggac
    45841 cttactgtat atattgatgg gtatttctct ttatttgtta cttaatcaca acaacaaaaa
    45901 acagcgaaac aatcttgtcg gtctttttgt gattcagttc atttttaatt ttatatggtc
    45961 tgctctattt tttaatttaa gaaatatttt cattgcagca atcgatatta cacttttagt
    46021 tattttcttg tcagtgctta tgtatcagtt gtggctacat catcgcctgg caatgtggct
    46081 catgattcct tattatttat gggtactatt cgcaactcta ttgaattatt caatttattt
    46141 tttaaactaa gcgctaaaaa gagacgtcct gtaatttaac acatagaaaa agtgccacct
    46201 aagttagatt ttttgtctaa tttaggtggc actctttatt aaccaaacta tttttttcta
    46261 gtacccactt gttgttccat actcattatt ctggtcataa ccataaccat tttgttcctg
    46321 tccagcgtaa ggatcataac tttgagtatc ttgacttggt gtatagtact gacttgtatc
    46381 ttctgtttgc tgataatagg tattcggatc ttggtactga tcatatgtgt tttctgaata
    46441 ggaagaataa tcggtgtacc cttcactatt cgatccatca ccgtttgtcg aatcgccatc
    46501 ttccgaatca tcaaagaaca tcattgaagc attctcattt ttcaaattag gtaatgtatc
    46561 gcttgttttt atatttaatt gtttcttcag ttcattttgg atcgaaagaa gatcattttt
    46621 tcctaaaatt tgatagtaga tatcattgat catttgtcct tcgccttgca actggtcttg
    46681 tttgattgtt tcaaacgcag gggtgtagtt cgtcgctaca tcaagcatgt catcccaatc
    46741 taagtccgtc gtcacattgt tctcaactgc ttttaaaatt tttctataat tgcttacgcc
    46801 atctaaactt aaaactttat tgacgatttt cgtcactact tcccgctgac gacgttgtcg
    46861 accgatatcg ccttctggat cttcgtgtcg cattcgagaa taggctaaac ctttttctcc
    46921 attcaatgtt tgttcaccta ctggtacatg gacaccatcc aaagtaaagt caattttatt
    46981 attcactttg atgccaccga ccgcattgat caaatcttcc atgccatcca aattgatcgt
    47041 tacataatga tcaataggaa tatcgagcag ttgctcaatt gaatccatgg ccatttcgac
    47101 accaccaaat gcataggcat gatttaattt atctaccgtt ccataaccta cgatattcgt
    47161 ataaatatct cggtccaaac tgatgatcgt tgattttttt tgtttcggat taactgttac
    47221 taccatcatc gtatcagaac gtccttgttc cgttcttcct aatccaccgg tatctaaacc
    47281 taatagcaaa accgaaaaag gttctttgtc atcaatgctt actctctcag aacgtttgga
    47341 ggatattcga ttagagtttt tagaaatttt gtttactgtt tggctcgctt ctcccataaa
    47401 tctgattccg tatgctgata atccggcaat cagcaaaaca aataccccta acaaggttaa
    47461 tagaactttt tgccatcgct tcataataca cctctgtttc acattttcta aaatattgtc
    47521 tcatataact gttcaatcag caaattcact ttttaatcat aagaaaactt taaaaaacat
    47581 ttttgtgtaa cgattactat tttttaaaat ttctcaaaat tcttaactat cataccatac
    47641 cgtgaaaaag atcgaaagac cgaatacacg cagttcgcaa tcgttttatc tttttttacg
    47701 tattttttcc agaaaaagcg aacaacaagg tattttccaa ataatcgtgc agtaaaatga
    47761 tcgcacatac ctttcccatc tttttaacag tctttgccta ccgttcagat tatcattcga
    47821 tttcacgaaa atttcttcct atgactggtc cttttgttaa acccatcttc tattcgtttt
    47881 atttccagct cactgcatgg ctcttaaaat gactcgaact agtgatttca ttaccatcat
    47941 cttttaatgg atagatatgc tcctcttcaa agtaaaacga ttttctttta aagtaataga
    48001 attgacatgg taatgagtat gtatactagt ggtttcacaa atggtctttt attcaacatc
    48061 caaaaactga aatttcttta aaatccatta ctaacgaaga aaatgttcct tgcttgataa
    48121 ttagttagga catgtcaagt gaacaaatgc atttacttag ccaaaaaatc taaaaacttc
    48181 ataaatagac aaaaagacac gactctctaa gaagtcgtgt ctttattcaa caaattatta
    48241 taatttagtc acttcagcag cttgaggtcc gcgtgcacct tcaacaatcg tgaattctac
    48301 ttcttgacct tcttctaggc tcttgaagcc gtctccttga attgctgaga aatgaacaaa
    48361 tacgtcatct ccgccgtcta ctgtaataaa cccaaatcct ttttcattgt tgaaccattt
    48421 aaccgttccg tgttccataa atgtatagcc tccaaaaaag ttcttgcaag taaccttgca
    48481 agaacattat acccaattta gaaaacgcta gcaagctttc gctcttttat tttccaaatt
    48541 atttttaaga ctgatatgac ggaggaaatg gttattttgc tgcttcgaaa cgtttgttta
    48601 cttcatccca attaataaca ttccagaatg ctgcaatata atctggtcgg acatttttat
    48661 atttcaaata ataagcatgt tcccaaacgt ccaaacctag aattggtgtc tttccttcca
    48721 tcaatggaga atcttgattt gcagtagagg taatagctaa ttttccattt tccattacaa
    48781 gccaagccca tccagaacca aatcgtccag cggctgcttt tttgaattct tctttaaaag
    48841 aagaaaaatc accaaaagct tcattaatcg cttcttttat ttctcctgta ggttcgccac
    48901 cagcatttgg tgccatgatt tcccagaaaa atgaatggtt agcatgtccg ccaccattgt
    48961 tacgtacagc tgtcttgata tctgttggaa tagcgtccat atcagataat aattcttcta
    49021 ttgttttttc gcctaattct gggtatttct caattgctgc atttaaattc gtcacataag
    49081 tattgtgatg cttatcatga tgcagatgca tcgtttcttc gtcaatataa ggttccaatg
    49141 cgtcatatgc ataaggtaga tctggtaatg tgtaagtcat aaaaaaattc ctcctcaatg
    49201 ttttctttat aatgtctaca ttacaaagag taacaaagag aaggaaattc ttcaattgtt
    49261 atgcctagtc gtctttttca ctttgaccta cttcttcttc aaaaacctca gtttctttaa
    49321 aaataaacaa cttgctgaaa atatagttcg ctaggataac aataatttga gtgatgattt
    49381 tcgccacata ctcattgctg ttcataacat tgatcaacaa taccattgcc cccatgtcca
    49441 atccgtaaga aaggatacgg tagaagaaga acttaccgca ttcaataagt gcatgtgtaa
    49501 aattcggtga ttttgaatgg aatacccaaa gtttgttcgt tacaaaagca aacaatacag
    49561 aacaaaacca cgaaatagaa ttggataagg ccatacttaa atcaaaatat tggcgtgcga
    49621 gagcaaaact aacaaaattt acaatcgtag ccaaaccacc aaaaaagaga tagactagta
    49681 tctcccaata tccttttgct tctaaatatc cttttaactg tcgatactgc ttcatgagcc
    49741 aacctacctt attaaaaatt cttatttatc atactctata tttcaataac tttcatcttt
    49801 atttaaaaaa attaattttt tttgatcctt tgcctttgtc tctgcttgga tcgtcacatg
    49861 tgtaatacca tattttaatt ttaataaaga aattatacga ggatatatct tttcaatctc
    49921 gcttagtagc tgatcttcaa aatcaatatg tccgtcgaaa ataattatct tttcgctata
    49981 ctgccaaata tggacatgat gaacattctt taccccatcg attgcttcaa tttcttgttc
    50041 cattttttca tagtcaatag caggtgcact ttgtatcaaa atagcggcag cttttttgat
    50101 caccattatc gcttctatca atatatatat cgaaatgatc agtgtaataa ctggatcgat
    50161 ccagtagata tcgaaaaaac ggatcaaaat tgctccgata atcacactta ccgaagacag
    50221 tgtatcactt aacaaatgca aataactaga ttttatattc aaactttcat ctgcttcttg
    50281 tcgtaataat aatactgaga acaaattcgc gagcaatcca ataactgcta ctgttagcat
    50341 caaatgacta tttatctttt ctggtgaagt gaaccgcttg aatgcttcaa cggttaaaaa
    50401 caaggataat atgatcaata tgaatgagtt gatgaaagca ctcaatattt ctaaacgctg
    50461 gtagccatac gtacgttttt tatttgcttc ttttcgggcc attttttgtg ccccgtaaga
    50521 aaaaataatt gccaatgtat cactaaggtt atggaaagca tctgaaacaa gagacaaact
    50581 ccctgacata atccctccaa taaattctga taaagtgatc accgcattca aaaccactac
    50641 tccaaatagt ttttttgatg atctctgtgg ctgtgtaggc actattcctt cctccatttt
    50701 atttatattg gatagtcttt agacaagaat aacatttttt tgatatttca ccaatcattc
    50761 agatgtttcc ctaaagcact tgtatttcac ttgatacttc gctagaattg caagtgatga
    50821 cactactaga tgaggagtac ttatgacaac aaaacaatta attatcagtt cctttatacg
    50881 attcaaagac ctaaaggagg cgagaaatgt cccattttgg aaagccattg tctatctttt
    50941 agttttgagt ataattatgg ctttgccaat ctcttttcaa gtttttcaag tcttaaataa
    51001 cattcaattg gatgggcaaa agattgctac tcgcattcct gattttacaa tccaaaacgg
    51061 gcagcttcaa accgaagaaa aagaaggatt catttaccaa acaaactcca ttatcttcac
    51121 atttgatcca gagggaaaac gaacagaaca ggacatctct actgatttga tggggaattt
    51181 tgttagtgtc gggatgttaa aaaacaaact tatcatagct cttcctaata caggcacgac
    51241 ttctgctttg ttgaacaata accaattaga acttccctac accaatgaat ctttaaaaaa
    51301 cttaacaggt aagcagctcc gttctttttt aagtgaagca tctataccac tttggataaa
    51361 agtcctgaca tttatatttt ctatttatcc tagttttctg aatctgctga tcactttgct
    51421 atttaccaac attgctgctt ttctctatgc acgctttcga ctaacaaaag ctacttttct
    51481 tgattgttta aaaacattga tctattcaat ttcattgcct actataattg caacaatctt
    51541 gatggtcttt cttccttctt ttgacactag cgccttcatt gcttttgcag gaatctttat
    51601 ttttgctcaa gctgtcaaag gatgggcaaa aatatcgatc aattaagaaa taaagacaac
    51661 tcgttatttc tacttaaata gaccctttta gaggttgtga catttttttg tcacagcccc
    51721 ttttttattt gaaaaatatt tctttctcaa aaaacaagca aggcgcttca aagttcttct
    51781 tgtatgcgct acacttagag taaagaaagg cgggaaatta actatgtcag aaagaatcat
    51841 ttatgcagat cgtccatttc gtgatgaatt tatcgaagaa atccatacta tagcgcctgg
    51901 ttatatcttt aaaacagaat tacaaccaga ggatctacaa aatgtagaga tcagtttggg
    51961 ctggaactca gactaccaag aaaaactatt agcctcttct accttgaaat gggtccaatc
    52021 tatctcagct ggtgttgatc agctacccct tgagactttt tcaaagaaac atattttact
    52081 ttcaaatggt agtggcatcc acagtgaatc gattacggag catgtcttag gaatcatcct
    52141 tgggtattct cgtggtcttt ttcaagcaca aaaggcacag agtgacatga aatggttagg
    52201 ttcatcggtc cattaccaat ctatccaagg gaaaaaaata ctaatcattg gaactggcca
    52261 tattggaaaa atgattgcaa aaaaatgcaa agcattagac atgaactgct atggaatcaa
    52321 tacatccggt catccagcag aaggaatgga aaagactttt tctttaaatc aactaaagga
    52381 agttcttcct gaagtagatt tcgtagtcaa tatcttacct ctaacggagg aaacaactgg
    52441 gctctttgat cagcatatgt ttgaacactt tgatccaaaa tctgtattca tcaatgttgg
    52501 ccgtggtgct tctgttaaga cacaagatct ggtgcaggct ttaaataacc agcaactttc
    52561 ctttgcagca ttagacgttt ttgaggaaga gcctcttcca caagatcatc ctctttggaa
    52621 aatggacaat gtactgatta cttcccacat tgcaggtttg acacctgatt ttcaaaagaa
    52681 attaatggct atttttttaa ccaatctgaa aagctacttt gccacccatg agcttcaaac
    52741 caatcaagta aaattgtctg caggatatta atagctgaaa acaatatttt caaaagaaaa
    52801 aagagaaggt gacaaaaatt gtgtcaccct ctcttttttt attgtttttt aaagtgatag
    52861 gttggctcct ccaggttggg atcataatca acaagtaaat catatccttt aaagaaccat
    52921 tcatctgctt cctcgataaa aaaaagtgtc ccatccattt ccactttctt tacaggatgt
    52981 tctggctgtt caactgacat tccaatcgaa aaaccatcat gaacctctgt tttgccgtag
    53041 acttttccaa aaaaacgtat tccgtaagtt tctggcagat caatttctga gtcaaaccat
    53101 ttttgtgctt ttggcgttac cgttaatttc atcccaacaa cctactttct cttttctttc
    53161 tattgattca tcttatcgtc tagaatatcc acatcgcctg tctctccaat aaccagtaga
    53221 ttatcatttt cacgaacata ttcatctgct gcaggagaag cgataaccgt ttgatctact
    53281 cttcgtatgg cgactactgt taagtcaaaa cgttgcctga aatttaattc agctaatgtt
    53341 ttgttgaaaa atttaggatt ggagactcgg atttctgcta aagaatattt atctgacagc
    53401 tctaagtagt ctagaatatt ctttgatact aagttatgtg caatccgtat ccccatgtct
    53461 cgctcagggt gtacgacacg atctgctccg atcttctcta gtacacgggc atggtactca
    53521 ttttgggctt ttgctaaaat attcggtacg cccatttcct ttaccatcag agtaactaag
    53581 atgctggctt ggatatcttc tccaatagcg acaattacat gatcgaaatt tcttatacct
    53641 aatgaacgaa gtgtcatctc atcctgtgca tttgccacaa cagcatgggt ggctatattc
    53701 atatattcat tgactcgatc ttcattactg tcaatagcca gtacttcttg tccagcttcg
    53761 atcagtgtac ggcagatgct tccgccaaat cgtccaaggc ctacaattgc aaaattttgt
    53821 ttcaccattt tcctccttta gtatcttctt ttctcagtat aacaaagcct ttccaaaaac
    53881 aaaagcaagc atcattattg taaaaaagcc aagaaaaata atagttattt tctcagcttt
    53941 tgattatcct ctttatcatc gtttttattt caccaaacca tgtttaaacg catagatagc
    54001 tgcttgagtt cggtcttcta cctctaactt agctaatata ttcgacacat gcgttttgac
    54061 tgttttcagt gtgataaaca attcatccgc gatttcctga ttacttttcc cttcagaaat
    54121 cagcatcaaa atttcatttt ctcgattcgt taattcttca tgcaatactg gatcatttcg
    54181 ccgactcatt ttttccatca tttttgttgt cacttcaggt tctagtactc gctcgccgcg
    54241 ttgcgtagcc cttatagcat cagctatttc atgtgctgta gacgtcttca acaaatatcc
    54301 tgctgctcct gcttcaattg caggataaac tttttcatca tcaataaaac ttgtgacgat
    54361 aatgattttg gcttttggcc aatcttttaa gattgctttt gttgattcga tgccatccat
    54421 ttcttccatg actagatcca ttaaaatgac atccggtcga agtgccatcg ccttttcata
    54481 gccttgacgt ccattttcag cttctccaat cacttcaatg tcttcttgga tagataaata
    54541 agaagagacg cctaagcgta ccatttcatg gtcatctact aataaaactt ttatcatctt
    54601 tatctatgcc tccttcatca aagggacctt ggtctccacg ctcgtacctt gccctttaaa
    54661 actaatgatt tttactgttc caccaatacc agctactcgt tctttgatat tgttcaatcc
    54721 ataacttccc gttttggttt cattcatatt aaatcctgtc ccgtcgtcaa tgatacgtaa
    54781 taaaagattg ttatctattt tatgcaaata gacttctaat tcattagcct tagcatgtct
    54841 taatgtgttt gataacaatt cttgaacgat tcggaacaag tgatcctcaa tggaactagt
    54901 taattttaca tcttctacct cccattttag cgaaatctgg attttattct gcaattcctt
    54961 caataactgc tcaatgcctt gttttaaact tttttcttct aaattgactg gacgcagatg
    55021 aagaagcagc gcacgcatct cagactggga agcgttgatg atctctgcca ccattttcaa
    55081 ctgcttacgg aacatttctg gtgtctcact tttttctgct tgctccgtca atgccgacat
    55141 catcatcatc gctgcaaata attgctgtga aactgaatca tgaagctcac gagctaatcg
    55201 atgcctttct aattttaaaa tctcttcttt ggtttgtcca tctacatatt gaggacggct
    55261 agtcataatc tgtaattcac tggatacttc gatcattttt tcttttattt tagtaatttc
    55321 catatcaata tctttgatgt acagatcatc cgacgcgctt gggatcggct gatgcaaaag
    55381 tttggattca taattgccag aagaaagtgc acgaagcttt tcttcgattt tcccgtattt
    55441 tgccttttga ataagtgata acaatagaaa tacgatcaat cctacaccta atgaaattgc
    55501 catcaaatgg aagataagcg gaacgtataa taaacgcgca cgcagcaatt ctccccacca
    55561 atgactttgg ttactggcat aaaaataagt gaaaagtgaa aatagaataa taaggaaagc
    55621 cgcaatcccc gaataaacag ctagcattgc tttggatatt cttcccatca tattcgtatc
    55681 acctcgacat ctccaaccaa agtattggta atgatcttca atctgcgagg attttcatca
    55741 taatcactgc tgcagatttt gatctgttcg ttttttaagg aaaactgctc ttcttcaaac
    55801 aagacatttc ctactaatgt cgcatgctct aaacgaatag ccactcccag gggtactaag
    55861 atccgtgttc tgcctatccc ttttcttaca atcacaatat tatcatcttt tggcaaaagt
    55921 gtattgccca aatcgatgat agtatcacca gaaatgatgg caatgttgat atcatcccat
    55981 tcatagacgt cgtttccaat acgttggtta ccaaataatt gctgtctttt acgctcatta
    56041 ttatgtgttt ttaattgctc tgtttggacc atcatgatct gtttttttcg ccaaaaagca
    56101 ttttttgtca aatctattcc tgaaatctct acacctttca gcccgataaa taaaacggcc
    56161 aatacaacca tcatccataa agcaggactg ttgactaagc taaaaaaaat gaccaagctc
    56221 cctaagatca attgaaaatt ttgaaattta gtacgaggat atttgcgcat agctagatat
    56281 atattgaaaa taccgaatat tactaaaagc aaaagtccag tattgtttac gatttgccaa
    56341 acagcaaata acagcagcag tgcttcgact accacaaaaa aacgccaaga actattcatc
    56401 gctttttctc cctcctgaca cattctcatt tcatcatacc tatttttttg actgttggct
    56461 ataggacttt agatgtaaga tcgttcagtg aaacctgcaa tactcaaaga gaaaagatct
    56521 agagtgcgtc atcagtaaga tgaactctct agatctttca gtcagactac gcttgttcta
    56581 ctttgacgat tttaactaac atatcgccac ctggtgtagc aatggttact tcatcatttt
    56641 tacgtttgcc aatcaatgct tgcgcgatag gagaatcatt ggaaatcttt ccagaaaacg
    56701 ggtctgcttc agcacttccg acaatcgtgt actcttcttc atctccatca ggcaactcga
    56761 tgaacgtaac tgttttaccg atcgatacct catctgcatc tacgccgtca ttatcgatga
    56821 tttgtgcaaa acggatcata ttttcaattg ttgtgattct tccttcaaca aatgcttgtt
    56881 catctttagc tgattcgtat tcggagtttt cggaaagatc tccgaaaccg cgagcgattt
    56941 tgattcgttc aatgatttct ccgcgtttaa ctgtttttag ttcttctaat tctttttcta
    57001 atttttcttt accttctaga gtcattggat aaactttctc taccatgtgt aacgtctcct
    57061 tttttcgata aaataccgca aaaggtaatc ttttgaggaa tatagtccaa aaaagattac
    57121 cacgcttttc ttaaaatgta aatgtttttc taaaaatgat caatgactag cccctgtttt
    57181 tctaagaatt atttacatat ttttgcacta gttccatatg ttcttcatac gttttagcga
    57241 aataaacatt tccagtgctc gtatctgcta cgaaataata atagtcattt tctgctggtt
    57301 ctaatactgc cttgattgcc tcttcgcttg gattgtcaaa aggaccaggc ccatagccgg
    57361 tattggtata caaattgtat ggtgaatcta cagctgtatc ttcatacgtc accaattctt
    57421 tatgttctcc taatgcatat aaaacagaga tatctgactg aagtggcata tttgccttga
    57481 ttcggttaaa gaagacttgt gcaatatttt tacgatcatt ttcttttacg ccttcttttt
    57541 caactagaga agccaaagtt aaaacttctt gtacagtcaa atttttctgt ttgatagctg
    57601 aataatagtt ttgcatgacc gtatttgttt tgttcaccat ctcaatcaca agatccttaa
    57661 gcgaagtact cgtgtagtaa tcataagtag ctgggaataa gtagccttcc aaacggtatt
    57721 ttgtgttctc agctttagag gcactttcca acaattctgg gaatttttga tacaactcgt
    57781 taaagaaagt ctcatcattt actaaatcta aaaattcttt cttgtcttta ccagttacct
    57841 tcgctacccg ctcagcaatc tgatcgatgt cgtagccttc tggaatggca attttcccat
    57901 ctgcaacttt tgttggctca gaagtgccgc cttcttgcag ctgtttgcca atctcgtcaa
    57961 gagtcatatt aggcgctaac tgataatagc cagcttgaaa gccagttaaa tttttaaatt
    58021 ttgtataata attgaaaaca atcccgcttt taataatatt gtccttctcc aaaatctcac
    58081 caatctgctt gttagaagag ccactcggga tctccacttg gactaattgg tcatctgatt
    58141 tatctaaagg cttcaacccg ctatctacat aacgatacac ggtaaatcca agaaatccgc
    58201 caataattaa taatgtaaga gcaatcacta aaatgatctt cctcacaatc ttatcttctt
    58261 ttttacgcag atttctccgc ttgtcgatta tatctgctgt agctgcctta ctggcttctt
    58321 ttttatcttt acttgggtgt tgtttgtttc ctgacaaaac aactcctcct tactatcatc
    58381 ttcgtaactc tgtttattat acatgaaatt tctactgccg tgaattattt taccaaatat
    58441 tatgtcaaaa ttgtaagtgg caaatactct ttttgaggat tacaatgaaa aatagttaaa
    58501 aatggataga aaggaattaa catgtcaaaa ggaaaacatc gaaaaaaaca aaaaatttct
    58561 atttcttttc ttgtaatttt attattgtct gtcagcttct tttcaacagg catcattata
    58621 ggaggtcaat taacaaaaat acctcattcc caaacttata aagcggagcc aacacaactt
    58681 acttccaatt taacgcccga tgagcagagg cttgctagca aaattgatca attactacaa
    58741 aaatcagatt atatcggttc gatttatgtt cgcaaaaata atcgcgtact attagaaaaa
    58801 ggatatggat atgcaaatct gcgcgtaaaa gaatcgaatt caccttctat ttactatcaa
    58861 atcggttcga ttcaaaaagc aatgactgct ttactgatac taaaacaaat ccaagctggt
    58921 aagttaactt ttgacacaaa gttagcagaa ttttatcctc agattcctgg aagtcagcaa
    58981 atcacaatca aaaatatgct ttatatgcgc tctggtcttc atcgaactgc ttctcccgac
    59041 aaacctatgt cggatagtga aatcattcag ttcgcccttc atcatttgaa acttactgat
    59101 tatgatacgt atcattatga accattgaat tatacattgc ttacagggat attgatcaaa
    59161 ctaacgaatc agccttatga aactcttcta aaaaaagaaa tcattcgtcc actccatcta
    59221 gaacatacag atttttatga caatgtaaaa aattcgcctc aacatgctgt atcttaccag
    59281 atgtctgcaa ctgacgatta ttcaaaagca ttgatcgagc ctgaaaccga catccgaaat
    59341 gaattaggta caggaaacat aagtatgtct gtttatgact tgaacaaatt ctttaccagt
    59401 gttctttctg gcggtatcat accaaaagaa ctacttttct cattgtggca aaataatggt
    59461 gataaacatc cttacagcgg cggtgtttac agtggaaacg attatatcct tgctcagggg
    59521 aatatcaatc gattccatgc agtagtggcg atgaaaaaag acactaaaga tgcaatcgtc
    59581 atggaaagta atatccaatc ggataagaac atcaaattgc cggccactga tttaagaaat
    59641 cagatttatg aactgatgga aggtgttcac ttatcaaatt aaattttgac ataatattag
    59701 ttgtcatctc tgtatagtca atttagttag tactctatct gaaaagtaga aataaattaa
    59761 caaatattat ctgtccttat tatttgttaa tttatttctt atttta
//
LOCUS       DAFCNS010000006        59180 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFCNS010000006 DAFCNS010000000
VERSION     DAFCNS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.006"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..513)
                     /locus_tag="LNE23_000371"
     CDS             complement(136..513)
                     /locus_tag="LNE23_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930249.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            769..1497
                     /locus_tag="LNE23_000372"
     CDS             769..1497
                     /locus_tag="LNE23_000372"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBK6930250.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1497..1751
                     /gene="purS"
                     /locus_tag="LNE23_000373"
     CDS             1497..1751
                     /gene="purS"
                     /locus_tag="LNE23_000373"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBK6930251.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1753..2424
                     /gene="purQ"
                     /locus_tag="LNE23_000374"
     CDS             1753..2424
                     /gene="purQ"
                     /locus_tag="LNE23_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBK6930252.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2425..4647
                     /gene="purL"
                     /locus_tag="LNE23_000375"
     CDS             2425..4647
                     /gene="purL"
                     /locus_tag="LNE23_000375"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBK6930253.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4632..6071
                     /locus_tag="LNE23_000376"
     CDS             4632..6071
                     /locus_tag="LNE23_000376"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBK6930254.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6094..7146
                     /locus_tag="LNE23_000377"
     CDS             6094..7146
                     /locus_tag="LNE23_000377"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBK6930255.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7143..7721
                     /gene="purN"
                     /locus_tag="LNE23_000378"
     CDS             7143..7721
                     /gene="purN"
                     /locus_tag="LNE23_000378"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBK6930256.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7718..9253
                     /gene="purH"
                     /locus_tag="LNE23_000379"
     CDS             7718..9253
                     /gene="purH"
                     /locus_tag="LNE23_000379"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBK6930257.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9269..10543
                     /gene="purD"
                     /locus_tag="LNE23_000380"
     CDS             9269..10543
                     /gene="purD"
                     /locus_tag="LNE23_000380"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBK6930258.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10665..11579
                     /locus_tag="LNE23_000381"
     CDS             10665..11579
                     /locus_tag="LNE23_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6930259.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11712..13277)
                     /locus_tag="LNE23_000382"
     CDS             complement(11712..13277)
                     /locus_tag="LNE23_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK6930260.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSYFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13270..14721)
                     /locus_tag="LNE23_000383"
     CDS             complement(13270..14721)
                     /locus_tag="LNE23_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6930261.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14735..15751)
                     /locus_tag="LNE23_000384"
     CDS             complement(14735..15751)
                     /locus_tag="LNE23_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6930262.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15971..17059
                     /locus_tag="LNE23_000385"
     CDS             15971..17059
                     /locus_tag="LNE23_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBK6930263.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17113..18639
                     /locus_tag="LNE23_000386"
     CDS             17113..18639
                     /locus_tag="LNE23_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK6930264.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18641..19846
                     /locus_tag="LNE23_000387"
     CDS             18641..19846
                     /locus_tag="LNE23_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6930265.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20103..21080
                     /gene="guaC"
                     /locus_tag="LNE23_000388"
     CDS             20103..21080
                     /gene="guaC"
                     /locus_tag="LNE23_000388"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBK6930266.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21344..22768
                     /locus_tag="LNE23_000389"
     CDS             21344..22768
                     /locus_tag="LNE23_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK6930267.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23229..24338
                     /gene="pdhA"
                     /locus_tag="LNE23_000390"
     CDS             23229..24338
                     /gene="pdhA"
                     /locus_tag="LNE23_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBK6930268.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24341..25318
                     /locus_tag="LNE23_000391"
     CDS             24341..25318
                     /locus_tag="LNE23_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBK6930269.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25343..26986
                     /locus_tag="LNE23_000392"
     CDS             25343..26986
                     /locus_tag="LNE23_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBK6930270.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26994..28400
                     /gene="lpdA"
                     /locus_tag="LNE23_000393"
     CDS             26994..28400
                     /gene="lpdA"
                     /locus_tag="LNE23_000393"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBK6930271.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28562..28837
                     /locus_tag="LNE23_000394"
     CDS             28562..28837
                     /locus_tag="LNE23_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBK6930272.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28843..29610
                     /locus_tag="LNE23_000395"
     CDS             28843..29610
                     /locus_tag="LNE23_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBK6930273.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29778..31607
                     /gene="typA"
                     /locus_tag="LNE23_000396"
     CDS             29778..31607
                     /gene="typA"
                     /locus_tag="LNE23_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBK6930274.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31868..33640
                     /gene="recQ"
                     /locus_tag="LNE23_000397"
     CDS             31868..33640
                     /gene="recQ"
                     /locus_tag="LNE23_000397"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBK6930275.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33754..34056
                     /locus_tag="LNE23_000398"
     CDS             33754..34056
                     /locus_tag="LNE23_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBK6930276.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34059..35222
                     /locus_tag="LNE23_000399"
     CDS             34059..35222
                     /locus_tag="LNE23_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK6930277.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35248..38676
                     /locus_tag="LNE23_000400"
     CDS             35248..38676
                     /locus_tag="LNE23_000400"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBK6930278.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38697..39821
                     /locus_tag="LNE23_000401"
     CDS             38697..39821
                     /locus_tag="LNE23_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930279.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39811..40248
                     /locus_tag="LNE23_000402"
     CDS             39811..40248
                     /locus_tag="LNE23_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK6930280.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40232..40570
                     /locus_tag="LNE23_000403"
     CDS             40232..40570
                     /locus_tag="LNE23_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBK6930281.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40689..41249
                     /gene="rsmD"
                     /locus_tag="LNE23_000404"
     CDS             40689..41249
                     /gene="rsmD"
                     /locus_tag="LNE23_000404"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBK6930282.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41246..41737
                     /gene="coaD"
                     /locus_tag="LNE23_000405"
     CDS             41246..41737
                     /gene="coaD"
                     /locus_tag="LNE23_000405"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBK6930283.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41727..42770
                     /locus_tag="LNE23_000406"
     CDS             41727..42770
                     /locus_tag="LNE23_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBK6930284.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42929..43657
                     /locus_tag="LNE23_000407"
     CDS             42929..43657
                     /locus_tag="LNE23_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBK6930285.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43773..44018
                     /locus_tag="LNE23_000408"
     CDS             43773..44018
                     /locus_tag="LNE23_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK6930286.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44015..44233
                     /locus_tag="LNE23_000409"
     CDS             44015..44233
                     /locus_tag="LNE23_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBK6930287.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44369..44908
                     /locus_tag="LNE23_000410"
     CDS             44369..44908
                     /locus_tag="LNE23_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBK6930288.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44901..47174
                     /locus_tag="LNE23_000411"
     CDS             44901..47174
                     /locus_tag="LNE23_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBK6930289.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47212..48258
                     /gene="holA"
                     /locus_tag="LNE23_000412"
     CDS             47212..48258
                     /gene="holA"
                     /locus_tag="LNE23_000412"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBK6930290.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFASYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48347..48601)
                     /locus_tag="LNE23_000413"
     CDS             complement(48347..48601)
                     /locus_tag="LNE23_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBK6930291.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(49001..49270)
                     /locus_tag="LNE23_000414"
     CDS             complement(49001..49270)
                     /locus_tag="LNE23_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930292.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49740..50195)
                     /locus_tag="LNE23_000415"
     CDS             complement(49740..50195)
                     /locus_tag="LNE23_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBK6930293.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50333..52261)
                     /locus_tag="LNE23_000416"
     CDS             complement(50333..52261)
                     /locus_tag="LNE23_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK6930294.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52458..53291)
                     /locus_tag="LNE23_000417"
     CDS             complement(52458..53291)
                     /locus_tag="LNE23_000417"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBK6930295.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53483..54187)
                     /locus_tag="LNE23_000418"
     CDS             complement(53483..54187)
                     /locus_tag="LNE23_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6930296.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54210..54836)
                     /locus_tag="LNE23_000419"
     CDS             complement(54210..54836)
                     /locus_tag="LNE23_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6930297.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55162..57537
                     /locus_tag="LNE23_000420"
     CDS             55162..57537
                     /locus_tag="LNE23_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBK6930298.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57561..58052)
                     /locus_tag="LNE23_000421"
     CDS             complement(57561..58052)
                     /locus_tag="LNE23_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBK6930299.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58239..59159
                     /locus_tag="LNE23_000422"
     CDS             58239..59159
                     /locus_tag="LNE23_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6930300.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT        19401 a        10245 c        12737 g        16797 t
ORIGIN      
        1 taaccaaaaa agaagcagat ccgttagcgg ttctgcttct tttagtttat tatattttct
       61 tatcaataat aggaacctga ttttaaaagc ggctacctct ttaccgtgcc tctttacata
      121 caaaaaatag aggaattaag cgtaaatggc ttggctatta taactaaaat gctggttatg
      181 gagatgttgc agcaagtttt cgagaaaagc gtattccatc tcttgtccat tgaataagat
      241 ctggatacga taatgatttt tgaaacgctt ctgttcatga atctgactga ttgaaagttc
      301 gcctttgtgt tctgtctgat aaagaaaagt ccttttacgg cattcagcac agcgcttggt
      361 tgctcggcgg atttttcgga atgtataaaa actagtatct ttattgcata aataaacgtc
      421 tttgatcgac agcaaatcat tatatagaca ggaacgttct tcagctgtta attctgataa
      481 aaaaagctga ccttgtttta ttgcatcttc catcggcaag tgcctcctcg aacccattat
      541 ccaaattttt agttaattct aatataccat cattaagtat aaatgtaaac gcttgttgat
      601 ggtataagat tttcgtttct gataaaacac gaacattaaa agaatctaat gccttaaatg
      661 tacatatttt tgttgattta taaaaagatg attgctaaaa tttaaagtga aaaaacgaac
      721 aaaaaacgat gtatttgctt ttaatgtaaa taaaaaggag agacgtacat ggagaagaaa
      781 gaacttttgt atgaaggaaa agccaaaaaa atatatctta cttctgatga gaacttgatt
      841 tggatagaat atttagatca agcaactgcg ttgaatggcg cgaggaaaga ccagataagt
      901 ggaaaaggag aattgaataa tcaaatcaca agtgcaattt tccgttatct gactcaaaga
      961 ggaataaaaa atcattttat tgaacaattg tctaaaaccg aacaattagc aaaaaaagta
     1021 caaattatcc ctctggaagt ggttatccga aatattgcag caggaagttt ctcaaaacgt
     1081 ctaggtgttc ccgaaggtaa agaattcatc aaaccaatca ttgaattcta ctataaggat
     1141 gaccagttgg atgatccatt tatcaatgaa gagcatatct atttcttaca acttgcaaag
     1201 ccggaagaaa tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat tttgaccgaa
     1261 ttatttgcta aggtcggttt ggttttagta gatttcaagc tagaatttgg ccggtcgaat
     1321 ggacaaattt tgcttgcgga tgaagtaaca ccggatactt gccgattatg ggatgaacgt
     1381 acgaatgaac atatggacaa agacgtttac cgcagagatc taggtgattt gatcccagtc
     1441 tatcaagaag tatataaccg tctatcggaa ctaactgaag aaaaggagcg agtatgatgt
     1501 atttcgtaaa agtatatgta acctataaag attcagtgtt agatcctcaa ggggaggcag
     1561 taaaaaaagc agtccatcgt atgggatatg aaacaatcga agatatccga ataggaaaat
     1621 actttgagat acaacttgct gatgcagaag aaccaatcga tacagtgatt gaaacgattt
     1681 gtgacaagct gcttgcaaat gtaaatatgg aaacataccg atatgaaatc aaaaaaatgg
     1741 aggaagtata acatgaaatt tgcggtcatc gtttttccag gttcaaattg tgacgcagat
     1801 atgttatggg cagttcgcga gatcatgggc gctgatgccg aatttgtacg gcatgatgag
     1861 tcctctttag aaggatttga tggggtttta ctgccgggag gattctccta tggtgactat
     1921 ttgagatgcg gtgcgattgc tcggttttct acaatcatgg aagaagtgat tcgttttgct
     1981 gaagaaggga aaccagtatt cggtacatgc aatggattcc agatattgac agaagcagga
     2041 cttttgccag gtgtactgag acgaaatgag tctttgcatt ttatttgcaa aactgtacca
     2101 ttgaaagtca accccaagac ggcctttact tcagagtatg aagccgacga aataatctat
     2161 cttccagttg ctcatgggga aggaaattat ttctgtgacg aagaaacact tcaagaacta
     2221 aaaaaaaata atcagattgc ctttacttat aatgacaaca tcaacggcag cgtggaaaac
     2281 attgcaggga ttacgaacaa agcagggaat gtgttaggaa tgatgcctca tccagaacgt
     2341 gcagtagaag acttgctagg atcagaagat gggaaacggt tctttgcttc cattttaaaa
     2401 aacttcggaa aggtgaccgt atagatgaaa acattgaaag aaccgacagc tgaagagatc
     2461 aagaaaaaaa gaatctatgc cgaatgggga ttgactgatg aagaatatcg aatgatcgaa
     2521 gaagatatct taggaagaat gcctaattat acggaaactg gactgttttc tgttatgtgg
     2581 agcgaacatt gttcctataa aaattctaaa ccggttctac gcaaatttcc tactagtgga
     2641 cctcatgtgt tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg tgatggacta
     2701 gcagttgtct tcaaagcaga aagtcacaat catccttcag ccgtggagcc ttatgaagga
     2761 gctgcgacag gagttggcgg aatcatcaga gatattttca gcatgggggc tcgtccaatt
     2821 gcgttgctag attcattgag gttcggcgaa ttagataatc caagaacgaa atatttacta
     2881 gaagaagtag ttgcaggtat cggcggatac ggcaattgta tcggcatacc gactgtaggc
     2941 ggcgaaattg catttgatcc ttgttatgaa ggaaacccat tagtcaatgc aatgtgcgta
     3001 ggtttgattg aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg taattcgatc
     3061 atgtatgtag gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt tgcctcagaa
     3121 gaatttaatc aagaagaaga acagcaacgt tctgctgtac aagtaggcga tccttttatg
     3181 gaaaagctgc tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga ttctctaata
     3241 ggtatacagg atatgggagc agcaggactt gtttcatcta gtgcggaaat ggcttcaaaa
     3301 gctggctcag gacttatatt gacattagat gaagtcccac aaagagaaac tgggatgaca
     3361 ccttatgaaa tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt taaatcagga
     3421 gaggaagaaa aaatccaaga actatttcag aagtatgatc tagatgcagt cactatcggt
     3481 aaagtaacag acgacgggca atatcggcta tatcatcaag gagaagaagt agcaaatctg
     3541 ccagtcgatg cattagctga agatgcacca gtttatcata aagaaatgaa agaaccagct
     3601 cgaatcgtag aatttcagca gttagctcct tatcagccag ttatcgaaga acctggacaa
     3661 gtattgcttg atctgctgca gcaaccgacg attgcttcaa aacgttcaat ctacgaaact
     3721 tatgattcac aagtacaaac caatacagtt gtccgtccag gaagcgatgc tgctgtgatg
     3781 agggttagag ggacgaataa agcgttagct atgaccacag actgcaacgc gcgctatctg
     3841 tatttggatc caaaaatcgg aggacaaata gcagtggcag aagctgctag aaatattatt
     3901 gctagcggtg gtaagccctt ggcgatcacc gactgcctga actatggttc tcctgataaa
     3961 ccagaagttt tttgggaatt atcgacttca gctgacggaa ttgctgctgc gtgtgaaacg
     4021 ttaggaactc ctgttatttc aggaaatgtc tctttatata atgaaacgga tggtcaagca
     4081 atctatccaa caccaatgat tggtatggtc ggattgattg aggatcataa acatatcact
     4141 actcaggaat tcaaaaaatc aggggatcta atctatatat taggtaagac gtttgcagat
     4201 tttgatggca gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt gatccgaaac
     4261 tttgatctat ccattgaaaa gagaaaccaa gagttagtat tgacagccat ccaaaacgga
     4321 ttgattgaaa gtgctcatga ctgttcagaa ggtggcttgg caattgcact ggcagaatca
     4381 gctttcaaac atcagctagg gatatctgta cagtttgaat tgtcctctgc tcaattattt
     4441 gcagaaactc aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac acgattcgaa
     4501 gaaatgatgg gagatgctgc tgttttagca ggtaaggtga cggatgaagc gataatcgaa
     4561 atttcagcaa ctgacggtca gatcaaaata gaaactgcag tagcaagaaa gtgctgggag
     4621 gatgcaatcg tatgtcttat gaagtgaaga gtttaaatga agaatgcggg atcttcggtg
     4681 tttggggtca tccagacgcc gctagtgtgg catacttcgg gctccatagt cttcagcatc
     4741 gaggacaaga aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag gggcatcgag
     4801 atttggggct gctttctgag gtattcaaag atcaaagaaa attgcagcag ttaacaggag
     4861 aagctgcaat cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg gacaatattc
     4921 agccttttct attcaaattt catgatcagt cgatcggctt agcgcataat ggtaacttga
     4981 tcaatgctcg ttcccttaga aaagagttag aggaaaatgg ggcgatcttc cattctaact
     5041 ccgatacgga gatattgatg catttgatcc gacgaagtga agaaacagat tttttaaata
     5101 aattaaaaag tgctttgaat gaagtcaaag gtggctttgc ctatttatta atgacagaaa
     5161 cggcaatgat tgccgctttg gacccaaatg gttttcgtcc attagcaatc ggacaaatga
     5221 aaaacggagc atacgtgatt gcctcggaaa cttgtgcatt agaagttatt ggggcacgtt
     5281 ttataagaga tgtccagcct ggtgagatag tgatcatcaa tgacgaagga atccagattg
     5341 attgctttac gaaggacacg cagctttcta tttgttcgat ggaatatatc tattttgcca
     5401 gaccagattc aaacatcgca gggatcaatg tgcatactgc acggaaaaat atgggacgga
     5461 atttagcgaa agaatcgcca gtaaaagcag atatggtcgt tggcgtacct aattcttctt
     5521 tgtcagccgc aagcggttat gcggaagcta gtggaatccc ttatgaaatc ggattagtaa
     5581 aaaatcaata cattgcccga acatttatcc agccaacgcc tgagctaaga gagcaaggtg
     5641 ttcgaatgaa attatctgct gtacgaggag tagtggaagg taaaaaggtg attatggtcg
     5701 atgattcaat tgttcggggt acgacaagca gacggatcgt ccatcttttg aaagaagcag
     5761 gggcaaaaga agtccatgtg aggattgctt ctcctccttt gaaatatcca tgtttttacg
     5821 ggattgatat tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt gatgaaatca
     5881 aagaatgtat cggtgcagat tctttaggtt ttttaagtga agagggtctg atcgaatcga
     5941 tcggtctgga aaaagatgct ccgtattcag gattgtgtat ggcttatttc aacggagatt
     6001 atccgacgcc gttatatgac tatgaagaaa aatatcagga atcactaaaa gaaaaagtat
     6061 cgttctttta aaagcagacc aagaagggag tatatgaatt caatggcaaa tgcatacgca
     6121 aaagctggcg tagatgtcga agctggatat gaagtagtag aacggatcaa gaagcatgtc
     6181 aaaaaaacgg aacgtttagg tatgatgggc gctataggag gattcggcgg ttgttttgat
     6241 ttatcggtct ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg cgtggggacg
     6301 aaattagcag tggcaatcga agcagacaaa cacgatacaa tcgggatcga ctgcgtagcg
     6361 atgtgtgtca atgatatctt agcacaaggc gcagagccgt tatatttctt agactactta
     6421 gcaacgggga aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt agcagatgga
     6481 tgtgttcaag gaaatatggc gctgattggc ggagaaacag ctgaaatgcc tggtatgtac
     6541 tcaggtaatg attatgatct agccggattt gctgtaggga tagctgaaaa atctcaatta
     6601 ttgacaggag aaacgatcaa cccaggagat gtgttgcttg gaatagcatc tagcgggatt
     6661 cattcaaatg ggtactctct tgttcgcaaa attttctttg agcagcagca gttttcttta
     6721 tcatcaaaac tgccagaatt gaaaaaaaca cttggcgaag aattgcttga gccaacgaaa
     6781 atctatgttt cttccttgct tccgctgatc aaagaaaaga aaataaaagg agctgcacat
     6841 atcactggtg gagggtttgt agaaaatatc ccgcgaatgt taccagaatc tatggctgca
     6901 caaatcaaac ttggctcatg gccgattctt ccaatattcg gctgtttaca aaaatatggt
     6961 gaaattcctg agatggagat gtatgaaatc ttcaatatgg gcatcggtat ggtcctggca
     7021 gtcgaagaat cgaaagtaaa agaaatagta cacgaaattg agcaagctgg cgaaaaagtt
     7081 tatgaaatcg ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat aggagaagaa
     7141 cgatgagaat cgctgttttt gcttctggaa acggaagcaa ctttcaagcg ctagccgatt
     7201 atctatcgaa aaaaggattg gaatcttcga ttgactggtt gttttgtgat cagcctgaag
     7261 catatgtttt gaaacgtgca actgcgttga gtgtaccggc agattgtttt tcacctaaag
     7321 aatttgattc aaaaaaagag tatgaagaag caattttgca taaattgaaa gaaaaaaaga
     7381 tcgatttgat cgtattagct ggatatatgc gaatcattgg accggtattg ctgaaaaatt
     7441 atgataaaag aatcatcaat atccatccgt ctcttttacc tgccttccct ggtttgcatg
     7501 gcatacgtga tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt cattatatcg
     7561 accaaggtgt agatactgga ccaatcatcc ggcaggaaaa agtgagaatt gaacaggagg
     7621 acacgttcga ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc tatccagaag
     7681 tgattagtga aattattgaa aatggaggct atctgaaatg acaagagcat tgatcagtgt
     7741 atcagataaa actgggattg tagcattcgc ccaaggtttg attgaacaag gagtagaaat
     7801 catttcaaca ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa cgctttcgat
     7861 cgaagaagtg acagggtttc ctgaaatgat ggatggccgt gtcaaaacac ttcatcctaa
     7921 aatccatggt ggtcttttag gaagacgcga tctccctagc catatggagg caatggaaac
     7981 ggaaaatatc caaccaatcg actttgtttg tgtcaattta tatcctttca aagaaacgat
     8041 tatgaaacca gatgtcgaaa tagcagaagc tattgaaaac attgatatcg gtggtcctag
     8101 tatgctccgt agtgcagcaa aaaaccatga atttgtcacc tctgttgttg atccatctga
     8161 ctatgaagaa gtactttcag aattacgtca gtatgggcat acttcacttt ctttcagaca
     8221 aaaactagcg gcaaaagtct ttcgtcatac tgcggcatat gatgcactga tcgctcaata
     8281 cttaacggat tgggtggaag aaaaagaacc ggaaaaattg acacttacgt atgaacgaaa
     8341 acaaacgctt cgttacggtg aaaatagcca tcaagccgct gccttttatc agtccgctct
     8401 tctggaaaaa tattcgatcg cagcagcaaa acaactccat gggaaagaac tttcttataa
     8461 taatatccga gatgcagatg ctgcgctacg gatcatgaag gaatatgacg agccaacggt
     8521 agtcgccttg aaacacatga atccttgtgg tattgggaca gcatctacga ttttagaagc
     8581 gtgggaagca gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca tcgttttgaa
     8641 tcgagaagta gatctgccaa cagcagaaca gatgcataag ctgtttctag aaatcatcat
     8701 tgccccttct tatacacaag aagctttgga attactgcaa aaaaagaaaa acatccgatt
     8761 actgacagta gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg tctcagtctt
     8821 aggtggactt cttgtacaag atcaagatct ggcaatagaa aatgaagaag agtggaaagt
     8881 cgtgacaaaa cgtaagccga ctccagcaga agcaaaagca ttagcgtttg cttggagagc
     8941 tgtcaaacat gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga cagtaggaat
     9001 cggagccggg cagatgaatc gagtcggttc agtaaaaatt gcgattgaac aagcgcaaga
     9061 gaaaatggaa ggtgcagttt tagctagcga tgcttatttt ccaatggatg attcggttga
     9121 atatgcagcc aaacatggta taaaagcaat cattcagcca ggaggaagca tcaaagacca
     9181 agattctatt gatatggcga ataaatacga tctagcaatg gtctttactg atgttcggca
     9241 ttttagacat tagagaggag tcggataaat gaacattttg gtgatcggtg caggtggtcg
     9301 tgaacatgca ataagtaaaa aacttttagc cagtccgaag gtggatacgg tgtattgtgc
     9361 gccaggaaat ccggggatga caaaagatgg tatcaattgt gtcgctatca gtgagcgaga
     9421 tcattcagcg ttaatccatt ttgttaagaa agaggctatt gattggagta tcgttggtcc
     9481 tgaagttcct ttgttaaatg ggatcgtcga tgattttatg gcggaaggat tgaaaatctt
     9541 cggtccgaca aaagcagcag ctctgatcga aggttcgaaa agttttgcta aacaattaat
     9601 ggatacttat caaatcccga cagctaaatc cagaagtttt gaatgttacg aaaaagcagc
     9661 agcgtatatc aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct tagcagctgg
     9721 gaaaggtgta gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag aacaaatgtt
     9781 gaaccagcat cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt ttttggcagg
     9841 agaagaattc tcccttttgg cgtttgtccg taataccaag gtgtatccga tggtcatttc
     9901 gcaagatcat aagcgggcat ttgataacga taaagggcca aatacaggcg gaatgggtgc
     9961 ttattcacca gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg aagaagtttt
    10021 aaaaccagcg gctttgggaa tgaaagaact aggacgctct ttcacgggtg ttttatatgc
    10081 gggtttgatt gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc gtttcggtga
    10141 tcctgaaacg caagtcgttc tttctcggtt gaagagtgac tttgcgcaag taatcgatga
    10201 tttattagag aatcgtacag tagagttaaa gtggcagcaa gaaggataca atgtaggcgt
    10261 tgtggttgca gctgccggtt accccgaaga atatgagaca ggaatggttc taccagattt
    10321 tctggaagaa gagctttctg tttactatgc tggagtttcc tcaaataacg gcaaactagt
    10381 tggagcagga ggacgtcttt atttagtaga agcatacggt gagacgatca aagaagcaca
    10441 gaaaaaaatt tacaccgcgt taatggataa aacgacagat ggaacatttt atcgtacaga
    10501 tattggagaa aaagctgcca atagcgaact gatcgtgaag taaatcagct gcttccattt
    10561 ataaaaaata caacaaacat aatcggaagc gccgatttta gtgaagcctt ttcataaaga
    10621 gtgtggtaca cttttgttgt gtatataaat aaggaggagt tcagatgaaa aaaattcaat
    10681 taggtacttc tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt atcaacaatg
    10741 cggaagatcc agtaaaagtg attgagacag cttatgaaaa cggcattgat ttttttgacc
    10801 atgcagatat ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct ctagcaaaaa
    10861 catcaatcaa acgagaagat ctttttattc aaacaaaatg tggaatcgtt ccaggtgtga
    10921 tgtttgattt ttctaaaaaa catatcattg aatctgtaga aaaaagtttg acccgtctga
    10981 aaatggatta tgtggatgct ttacttcttc atcgtccaga tacattggta gaaccagagg
    11041 aagtagctga agcgtttgat gaattggaac ggtcaggaaa agttcgttat tttggcgtca
    11101 gcaatcaaaa accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa ccattagtag
    11161 ctaatcagct gcaatttggc ttgaaacata ctggaatgat cgatcaaggg attcatgtca
    11221 atatgacaga tgaagcaagt atcgatcatg acggtagtgt acttgattat tcccgattgc
    11281 atgatatgac gatccaagca tggtcaccat accagtatgg attttttgaa ggtgtattta
    11341 tcgggaacga aaaattccct gaattaaacc aaacattaga agaactagca gctaaatatc
    11401 aaacgacacc aactggcttg gcttctgctt ggattttacg ccatccagcc aatatgcaag
    11461 tgatcgccgg tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct tcagaaatta
    11521 atatgtctcg ggaagattgg tacgaattgt accgtgctgc tggaaacatt ttaccttaaa
    11581 gattgtcata agcaggtcaa aagggctata aattaaaaag acagaataac ttgaaagctg
    11641 ttttcagctt cgagatattc tgtctttttt tattgcaaat ggaaagagta tcattgtacg
    11701 gatttcgttt attagttatt tttgttttta aggaaaagct gtgtccgata ttcttttgga
    11761 gaaatccctt gttttttctt aaataaataa ctgaaatagt tagggtcatt ataaccaatt
    11821 tcaaaagcaa tatctgaaat acgatagtct gattgctgca ataatttttt tgctaattcg
    11881 attctttgtt ctgtcagata atcgatgaat gtttggttaa acgattgaga aaaaatcgta
    11941 ctgaaataag agggacttaa tgcaacttgt tgagcgaccc aagttaagga catatctgga
    12001 tcggtaaaat tttctttgat aaaatccaat gcatggttca aaattggctg atatttgttc
    12061 cttgaaggct gtgagggcag agcagttaag aaagaaatga acatataaac tgtcgaacgg
    12121 taaagcgagt cgtcggcgct gattgctaag cgttgactgg tggatgtgca atgaaccatt
    12181 tcttgcggga ttttcttttc tttcaggcag acatgctgga gtagcttagt caagacaaat
    12241 aaacgatata atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg ttcgatgtac
    12301 ttagatactt cgtttttcga taatgaagac aaatcaagtg agaggtcaaa gtaaaagaca
    12361 gaatcaactg gaagtttcgc ttcttgaatc aaatcctcat agctgataat ttgttcttta
    12421 agggaactgg cggtttcaag tatcatctgc gtgatctcaa aagatgcagg taattcgctg
    12481 attctttctg cgacaggacc aatggcgaca gaaaaattat ttttttgtcc cttttctaat
    12541 tcatggacca acgtttgggc tacctgataa ctggtctcga tcacattttc ttcaagagaa
    12601 tcaactatta aaaatttgat gaatcgagag gaaacagcag agaagatcac ttgttgattc
    12661 tccctaaata aaaacttcaa ataatcactg agatgagcgt aatcttcaaa gtctgtactg
    12721 acttggtttg ctgctaataa tacggtcatt tttttccctg ctaaagagcg attgaaagat
    12781 tcagcaagtt tcaatgtttc actgatcgta agtgagcctt tgaacaaacc gttcaagaaa
    12841 tgattttttt tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt ttgctgcaaa
    12901 tctaaagcag ttactgcttt ttctattgct tgtttcaatt cgctactttt aatcggttta
    12961 agcagataat catctgcctg tacctggatt gctcctttca tataatcgaa atcgtcgaat
    13021 ccactaataa atatgagacg agtccaagga agtagtttcc ttgcttcttt tgaaaaagtc
    13081 aacccgtcca tgaaaggcat ttggatatca gtcagaatga tgtcgggctg taagtcgata
    13141 attgaagaca gtgccatctc accatctcca gcttcgccga cataagtgat tggataataa
    13201 gaaccaagga cagttatttg ttcccgcaag ttattgcgaa tgaggtattc gtcttcgatg
    13261 ataaaaactt tatacatgtt tgtttcctcc aattgttttg ggagatagag ggatcatcag
    13321 tgtgacagtt gttccttctc ggtatttgct gtcaatcgtg ataggttttt gatcatttcc
    13381 gtaataaaga aggagacgct tgtttacgtt gtaaagtccg tagccgatac tgaaatctga
    13441 atcgatccct ttggctaact ctttacgtat ttctgccagt ttttctgcag ggatgccaat
    13501 cccgttgtct ataactttga attggatcat ctcatcaatt ttgcatgtgg taatacgaat
    13561 aaatcctttg tgacggacgt acttcgttcc atggtataca gcattttcaa tcaatggctg
    13621 caagatcatt tttaaaatag aaacaggaaa aagtgtttct ggtatatcga tcttatagtc
    13681 aagcatatgg ccgtatctta tttttaagat gactagataa tcattgatat ggctaatttc
    13741 tttttcaaga ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa agccggaaag
    13801 tgcatgggtc atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag aaataatggc
    13861 atccaaactg ttataaataa aatggggagt aatctgagct tgcaatacac gaacttcact
    13921 ttgtgctaaa tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta gatcttccgc
    13981 cattttattc atgctagcgg taagtgtttg taattccagt gtttccggca gggttaaacg
    14041 aaagccaagg ttccctgaag aaagttgttc tgacatctcg atcagctgat tgatcggttc
    14101 ttggaccttt ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa tggtactaag
    14161 aactccaagt tccactattg ttaatagaat gagcgagcgg acaagtgtcg tatttttttt
    14221 gcttgctaag tcgatctcca cacgaacaaa atcttgtaag atatctgaca gcagttgagt
    14281 gacagaatct acctgattga tgatctcttc attggtatcc ataaaatctt ttgccaagat
    14341 attattgatc agctcgtttt gataatcttc cagtgtatcg atcgtacgta gcgatacatc
    14401 taacgtacta atttctttaa gtgtatttgt attatttttg atctctcgaa tatcattcct
    14461 aagttcttct aggatatttt cctcattgaa ctcatctacg gttacttggc cagaaatcaa
    14521 gtcccacata ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat ctgctttttg
    14581 gatatttgcg atcgtctgtt gataaatcaa aagctgttgc gaatataaca gtatgctcag
    14641 tagcaaaggg ataacagcaa atagaagcat ggctttgttt gcttgggcaa aaatctgttt
    14701 tatgcttaaa ttttttgtca tgatacttct ccagtcaata atttctggtt gggatgttag
    14761 ctatattctg ctgggaaaag acagtctcgg aaataaaaat atctttaggt aatgtttttc
    14821 ctgtttgaat atttccaaaa taacgagtaa cggtattggc tacataccag ccgacatcag
    14881 gtttacactc gactacacaa ttgacgattc ctgagcgtag cttctcgatc atatcttctt
    14941 gtccatcaat agttacaatg atcaaatctt ctccaggttt gattgatgtt tcttttatcg
    15001 cagctaaagc accgtgagtc atttcatcac tatggctgta taatacatcg atttcggata
    15061 aatcatttgt ttctaaatat cgtttcatct ggtcttttcc tttcatccga ataaaatcac
    15121 cgacaattcg gtgaacaata gtcatcggag gtttatggtc gatggttcca tcttttttta
    15181 gggttggttt acgattgatt gattccaaaa atccttcgga gcgtagcttg gtcggtgtgg
    15241 agttctctga accagcaagt tctaggattc tgatttcctg tttctttgaa tttgtgaagt
    15301 gattgctgac aaataaaccc gcccgatttc cttctgcttt gaaactgggg ccaatgtggg
    15361 tgacgaaaag ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg acagggatcc
    15421 ctgctcgctt tgcttctttt aatacagagt cccacccgga ttcttgcaaa ggagtaaaaa
    15481 tgatcatatc cactttatag gcaatgaacg tccgtatatc ttggatttgc cgttcctgat
    15541 tcatgtagcc atttcggtag agaacttggt aatcttcttt ttcaagcgca gtgataattg
    15601 attcagtatg ctttttccgc cagctactct ccgttcctga ttgcgaaaaa ccaacgacta
    15661 gtgggtcagg tgcttccttt gtattaggtg tacaccccat catttggtaa gcgattacaa
    15721 caagggctaa aaaaaataaa acacgtttca ctttaaatca ctccatctgg atttgttagt
    15781 actatcttat cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga aaaaaatcca
    15841 agaaacttaa aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa gtaaatccca
    15901 taaaaaaatc aatgttaacg ctttctttat tagctatact cggtgtatca attgaaatgg
    15961 gggataaaaa atgaaaaaca aatttagaaa gattgttggg actatcgcta ttttgaccgc
    16021 aagcattcta ttaagtgcgt gtggcaattc aggatctgca gatgatagta cggggtatgt
    16081 aggaatcgct atgccgacca aatcagcaga aagatggatc gctgatggga acaacatggt
    16141 ctcggaatta gaaaaactgg gttataaaac agatcttcaa tatggagaag ataaagtaga
    16201 aaatcaagta gcacaaatag aaaatatgat tacaaaaggg gtagatacac ttgtgattgc
    16261 ttctatcgat ggttcggctt taactgatgt gttagctaaa gcaaaagaag cagatatcaa
    16321 ggtgatcgcc tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt acgcgacttt
    16381 tgataatttt ggagtaggtg tatcgcaagc agcttatatt gaagagcact taggattgaa
    16441 agaaggaaaa ggaccattca cgattgaatt atttggtggc tcgcctgacg acaacaacgc
    16501 actaatcaat tacaacggag tgatgtctgt tttacaacca tatatggaca acggtcagtt
    16561 ggtcgtgcca tctggacaga cgagtttcag tcaaatcgct acgcttcgct gggatggttc
    16621 tacggcgcaa gcaaggatgg ataatctgtt aagcgcaaat tatactgacc aaacattgga
    16681 tgctgttttg tcgccatatg atccaataag tttaggaatc atttcgtcat taaaaggcgt
    16741 ggggtatggg agtgaaagta aacctttacc tgtgattact ggacaagatg cgacagttgc
    16801 tggcgttaaa tcgatcattg ccggggaaca aacacaaacg atctttaaag acacacgtat
    16861 cttagcaaaa aatacgattg aaatgatcaa agcaatcagt gatggagaag aggttccggt
    16921 caatgatact gaaacttatg ataatggggt gaaaactgtc cctacgtatt tagcaaatac
    16981 agtatctgtc gataaagata attatcaagc agagctgatc gacaccgatt attataaaga
    17041 atccgatctg aaaaattaag aggaaccaaa caaggaacta aagagatcta agtcagagag
    17101 aggataagaa ttatggcgga ttgtatttta aaaatggcag atatcaccaa aaaattttct
    17161 ggaatccgag cgttagattg cgtgaatctg atgattgaac gtggggaaat ccatgcattg
    17221 tgcggagaaa acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg cttgtatcca
    17281 tatggctcct atgacgggga aatcttttat gatggaaagc tgtgtcaatt taaagatttg
    17341 aaagatagtg aagaaaaagg catcgtcatc attcatcaag aattagcact aagtccttat
    17401 ctttcaataa aagagaatat ttttctcgga aatgaacaag cagaaagagg cgtgattgat
    17461 tgggaactga cagaaaagaa aacatatgaa ctgctgaata aagtcggttt gtctatcgac
    17521 ccgaatcttc tagtatccca gattggcgta gggcaacagc aactagttga aatagctaaa
    17581 gcattatcaa aatccgtccg tctgttgatt ttggatgagc ctaccgctgc attgaatgaa
    17641 gaagaaagcg cgaatttgct gagattgatc cgagagttga aagagcaagg tgtaacctcc
    17701 atcattattt cacataagct gaatgaaatc gtggatatcg cagatcgaat caccatttta
    17761 cgggatggac agacgattga gacactagaa aatgaaatga tcagcgaaga gcatattatt
    17821 cgcggaatgg tgggcagaga tttagttgat cgttatcctg aaagaaaacc ggatattgga
    17881 gaagtttatt ttaaagtaga agactggacg gtctatcacc ctcttgatca cgagcgaatc
    17941 ataaacgacc acatcaactt gcatttgaaa aaaggagaga ttgtaggaat agctggactg
    18001 atgggagcgg gcagaactga attcgctatg agtctatttg gacattctta tggaagcaag
    18061 atatccggac gtatattaaa ggaaggggca gagatatcag ttaagaccgt tccttcagcc
    18121 attgcaaacg gactcgctta tgtctcggag gaccgaaaat ctttaggact gaatctgctg
    18181 atggatattc gggaaaacgt gacgctgagc agtttggata aaataagtca aaaaggagta
    18241 ttagataaag aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat gcgtataaaa
    18301 acaaactcca tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca aaaagtcgtt
    18361 ttatccaagt ggctgatgac acaacccgat attctttttt tggatgagcc aacccgtggg
    18421 atagatgttg gggcaaaata tgagatctac acgattatcg aagaaatggc agcaaatggc
    18481 aaagctgtct gtcttatctc ttctgaactt cctgaaatat tagggatgtg tgatcgaatc
    18541 tatacgatga acgaaggacg tattacagga gaagtctcgc gagaagatgc agatcaggaa
    18601 gtcttgatga aattaatgac gaaagaagag gtgggttaag ttggaacagg caaaaaatct
    18661 tacggataaa aaagagaaaa aactaaattt gaaaagtaga gcagctgatc tttttggtcg
    18721 ctacagcatg gtgatcattt tagcgggact acttattgcg tttcaattga tgacggatgg
    18781 tattttctgg cgtccattga atataactaa ccttgttctg caaaatagcc atatattagt
    18841 actagctgca gggatgttat tagttgtgct cttaggtcat gtggatttgt cggtagggtc
    18901 agtgatggcg tttgttggtg caattgcggg catgatgatg gtcaatcgcc aaatgaaccc
    18961 gtggatgaca gttatcgcat gtcttgcagt aggcgggctg atcggcgcat ggcaaggttt
    19021 ttgggtagct tatgcaggga taccggcgtt tatcgtgact ttagcaggat tgctgatgtt
    19081 tcgtggactg acacaagttg ttctaggagg ccaatctcta gcaccttttc cagctgtttt
    19141 ccaaaaaatg tctacgggtt atttaccaga cttaagcgaa ggaagcagcc ttcacttctt
    19201 gacattgatt ttaggaatga tcatcacgat cgtgttagtt gttggtcaat ggcgaacaag
    19261 aaatcgtcgg aaaaatcatt tatttgaagt ggaatctcca acagctttct tggtaaaggc
    19321 ggtatttaca gcagtcgttg tactaggaat aacttatatc tttgcctctt atcaagggta
    19381 tccgattatt ttgatcattc ttggtttgat cgttgcagca tatgggtttt tgacaaataa
    19441 aacagtagca ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg ctgaactatc
    19501 agggatcaaa acaaagcgaa tcacgttctg ggtattcgtc aacatgggag tcatggcagc
    19561 tttagctgga ttagttttag ctgctcgatt gaatgcggca acacctcaag caggtacatc
    19621 gatggaactg gatgcaatgg cggcagtata ttttggtggt gcttcgactt ccggcggcat
    19681 tggaacaatc atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga acaatggcat
    19741 gtcgatttta ggcgtcgggg tagactggca gcaggcaatc aaaggattga tcttgctttt
    19801 agccgttgtg ctagacttgt ataataagaa aaaacgaaca acgtaggaga acgccggtta
    19861 ttcagaaaaa agactgtgac aagacatttg tcacagtctt tttttcgcta aaagcgaaca
    19921 ttcattgttt cgtttgtata gactaaaaat aatatatgtt tttttaccat tcgtataaat
    19981 tctctttttg ctcttgattt ttttgcttaa ttgagttaaa ttacataggg cgctaaaaaa
    20041 agccttgatt aaattaataa tgttcgtgtt ttgctggatg tgaacatcag gaggataatg
    20101 taatgaaagt atttgattac gaagatattc aattaattcc taataaatgt atcgtaaata
    20161 gccgttctga atgtgataca acggtcacat taggtaaaca tacattcaaa atgccagtag
    20221 tgcctgccaa catgcagacg attattgatg aaacaatcgc agaattctta gcggaaaatg
    20281 gttatttcta tatcatgcat cggtttgatg aagcagctcg aattccgttt atcaaaaaaa
    20341 tgaaaaagcg tggattgatt acctcgatca gtgttggcgt caagaaagag gaatattcct
    20401 ttatagagaa gttggcagaa gaatcgctaa atcctgatta tatcactatc gacatcgcgc
    20461 atggacatgc taactcagtt attgatatga ttcagcatat taaaaaatat ttgcctgaaa
    20521 cttttgtgat tgccggtaat gttggtacac cggaagccgt ccgtgaattg gaaaatgcgg
    20581 gcgcagatgc aacaaaagtc gggattggtc caggaaaagt atgtattaca aaaatcaaga
    20641 caggctttgg gactggaggc tggcaactgg cagctcttag atggtgtgcg aaagcagctc
    20701 gcaagccgat catcgctgac ggtgggatca gaacacatgg cgatattgca aaatctgtcc
    20761 gttttggtgc gacaatggtc atgattggtt cattgtttgc cggtcacgag gaatctcctg
    20821 gcgaaacaaa agtcgaaaat ggaatcgtgt ataaagaata ctttggttct gcttcagaat
    20881 tccaaaaagg cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa cacaaaggat
    20941 ctttgaaaga tacactagtg gaaatgcaac aagaccttca atcatctatt tcttatgctg
    21001 gaggaagaga tctggaagcc attcgaaaag ttgactatgt catcgtgaaa aattcgattt
    21061 ttaatggaga cacaatctaa aaaataaata aaatattagg atgttgtgaa aaagttgtcc
    21121 tgcattaagt aataagaaca gccaaacaaa aatagttctt tatatgtttt gtttggctgg
    21181 aattattatc gcagatacaa gcttttgaac atcattattc agaataaggg actgtgataa
    21241 gatttttgtc acagtccctt atgttttgca aaaaaagttt ttcttttttc tcgtaaattc
    21301 ggtaaaatca aaagaaaaaa tagacaatag aaaagagaag cttatgtggg aaaaagtaga
    21361 gaaaaaacaa ggaccgattt atcgtcaaat tatggaacag attatgagag aaatcgaaaa
    21421 cggaaggatc aatcctggaa gtcaacttga atcagaacga aaactagggc aactttttgg
    21481 tgtgaatcgt tccaccgttg tccatgcatt ggaagagctt agggagcttg gggtactaac
    21541 gagcaaacgt ggcagcggat ggtttgttaa tcagactgaa tgggggaaat tcgctgttcc
    21601 aagaatcgat tggcggcaaa tgatctctcc tcgttatgag cagacggact ggtatgagca
    21661 aaaaataaga gaagcaaagc agatccaaaa aacatcgttt cttgatttat acgctagcga
    21721 gatgccagat gagcttttgc cgaattttga gtttccttct tattctcttg aagaaatctt
    21781 agcggaagaa aaagaaatgg atcttttagg ctatactccg ttacgtaaca aaatcaaacg
    21841 gtatttggaa agtaaatttg aattggcatt ttcccccaac cagcttcttg tgactggtgg
    21901 cggtcagcaa gcaatctttt tgattttaca aacgattctt tcagctggag aggggatagc
    21961 tgtagagtca ccttcttttt tttaccgatt acctcttttc aaagcttcag gaacgcgttt
    22021 atttgggata ccgatggatg aagaagggat cgacttagaa aaactgaaag aatcgatcca
    22081 taaaaataaa ttgaaagcag tgcttatcaa cccgaatttt caaaacccta caggaaaagt
    22141 gatgagtcaa aaacgacgag aaaagctagt agaattgtgt cgacagtatc ggcttccaat
    22201 tattgaagac gatgtattta cggatctttc atttctaaac caagaagaag tccttcctat
    22261 acgaacgatt gatccggata atgtgttata cgtcggttcg ttatctaggg ttttaggaaa
    22321 aacaacgaaa atcggttggg tgataggacc tgagactttt atctcgcaac tagctcaagc
    22381 gcaagaaatg atggagtttt cgatgaatat ccttacacaa atcacagtgg ctaatgtatt
    22441 taatgaagca attgatttga aaatgcaaga tgtaagaaaa aagttgaaag aaaaaagccg
    22501 actgataagc gcttggggag attcacaatc gtttttcaag cttcattcaa ttttaggtgg
    22561 ttactatgcc tggattactt gggatgggaa aaaactagac cccgaactag cagaaaaagt
    22621 agtagatatg ggtcttggaa ttgcaccaag tctacttttt gggcaagata taaatggaat
    22681 cagaatcaat tacagtaggc tggatgaaaa ccaaataccg ttgttttatt caaagatgaa
    22741 aattctagaa aagtggttaa cagaataatt tcacaaagta aatagattaa agaatataac
    22801 agcttggatt taaatttcaa gctgtttctt ttttttcgga aaaagaatac gatataaaga
    22861 aactaagaaa aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt
    22921 gcaacaaaac tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt
    22981 agaaaataga aaaaatgcag atgaaatata aaacgctttc cttattgtga ttttattcac
    23041 taatgctgtt aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta
    23101 gcttgtattt ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca
    23161 gttttattca ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg
    23221 tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca
    23281 atgcagattt cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt
    23341 tagtacctga tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc
    23401 gtgtattaga ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac
    23461 caactgctgg acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg
    23521 ttcttcttcc aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc
    23581 aagctttttt atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag
    23641 ctctaccacc acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag
    23701 gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat
    23761 cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat
    23821 tttatatcca aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa
    23881 aaacacttgc acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg
    23941 atccgcttgc agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg
    24001 gaccagtcct aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg
    24061 atccaacccg ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga
    24121 tccgcttccg taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag
    24181 tcatcgaagc aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa
    24241 aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag
    24301 aacaaattgc aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat
    24361 ccaagcaatc acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt
    24421 tggtgaagat gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa
    24481 gttcggagaa gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag
    24541 tttcggtctt gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat
    24601 tttcgaagca atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg
    24661 cactagaaat ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga
    24721 attgcattcg gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat
    24781 tccttccaac ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc
    24841 ggttgttttc ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga
    24901 agcttacgaa gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat
    24961 tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga
    25021 aaatatatct gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat
    25081 cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc
    25141 gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc
    25201 cccaatcgga cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt
    25261 ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga
    25321 aaagaaggga cgaaacaaag tcatggctta tcaatttaaa ttgccggata tcggtgaagg
    25381 aatcgcagaa ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga
    25441 tgatacatta ctagaagttc aaaatgacaa atcagtagaa gaaatcccgt cacctgttac
    25501 aggaacggtt aaaaatgtga ttgttccaga aggtaccgta gcaaatgttg gggatgtatt
    25561 agtagaaatc gatgcaccag gacacgaaga caatgaaggg gacagtggtg tagccgcaga
    25621 atcccaaact ccagctaaac cggctgctga acccactgtt gatacagaat cagctggatc
    25681 gtcaagcgaa ggagtcttcc aatttaagtt accggatatc ggtgaaggaa tcgcagaagg
    25741 tgaaatcgtc aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact
    25801 agaagttcaa aatgacaaat cagtagaaga aattccgtca cctgtcacag ggacagttaa
    25861 aaacgtgatt gttccagaag gcactgtagc aaatgttggg gatgtattag tagaaatcga
    25921 tgctcctgga cacaatagtg caccgtcaac tagtgcgcca tcagctgaag caccaaaaga
    25981 aaaagtggaa acatcaggtt cagcaagtgt cgttgaagct gccgatccaa ataaacgtgt
    26041 attggctatg ccatctgttc gtcaatttgc ccgtgaaaaa gatgtagata tctcacaagt
    26101 aactgcaact ggaaaaggtg gcagagtaac aaaagaggac atcgaaaact tcttggcagg
    26161 tggtccaagc agtgcaccag cgaaatcaga agcgccagaa gcggcagcac cgaaagaagc
    26221 agctcctgca gcagaaagca aaccagctgc accagcaaaa ccattcaagt ccaacttagg
    26281 agacttggaa gaacgtgtag caatgacgcc aacacgtaaa gctatcgcta aagcaatggt
    26341 caacagtaag catacagctc cacacgtgac attgcatgat gaagtagaag tgtctaaact
    26401 atgggataat cggaaacgat ttaaagaagt tgctgctgca aatggtacga aacttacttt
    26461 cttgccatat gtcgtgaaag ctttaacagc gactgttaag aaatacccag tattgaatgc
    26521 atctatcgac gatgcaaacc aagaaatcgt ttataaacat tactacaata tcggtattgc
    26581 aactgatacg gatcacggac tatatgtacc aaatgtgaaa gatgcagacc gcaaaggaat
    26641 gtttgctatt gcagacgaaa tcaatgagaa agcaaaatta gctcatgacg gcaaactatc
    26701 tgcagaagat atgcgcaatg gaacaattac gatcagcaat atcggatctg taggtggcgg
    26761 ttggtttaca ccagtcatca actatccaga agtagctatt ttaggtgtag gaacgattgc
    26821 acaacaacca atcgtaaatg ctgaaggcga aatcgttgtc ggtcgtgtga tgaaactgtc
    26881 attaagtttc gatcaccgta tcgtagatgg agcaactgcg caacaagcaa tgaataatat
    26941 caaacgtctt ttagctgatc cagagctatt aatgatggaa ggatgataaa caaatggtag
    27001 ttggagattt tgctgtagaa ttagatacag ttgtaattgg tgcgggacct ggtggctatg
    27061 tagctgccat ccgcgctgct gaaatgggac aaaaagtagc aatcattgaa agagagtata
    27121 ttggcggtgt ttgtttgaat gtcggctgta ttccttcaaa agcactgata gctgcaggac
    27181 accattatca agagtcacta gattctgaaa tgtttggagt aactagtgaa aacgtcaaac
    27241 tagattttgc aaaaacacaa gaatggaaag aaaacaaagt tgtccatacg ttgacttctg
    27301 gtgttggttt cctattaaaa aaacataagg ttgaaacaat cgaaggagaa gcgttcttcg
    27361 tagatgatca cacactacgc gtcattcatc cagattcagc tcaaacttat tcgttcaata
    27421 acgcaatcat cgcaacaggt tcccgtccaa tcgagattcc aggatttaaa ttcggaggac
    27481 gtgtgttaga ttctactggc ggattagcat tgaaagaagt acctaagaaa tttgtgatca
    27541 tcggtggcgg tgtgattggt gccgaattgg gcggagcgta cgcaaactta ggagcagaag
    27601 taacgattct tgaaggctca ccacaaattt taccaactta cgaaaaagat ctagtaaaat
    27661 tagtagaaga tgatttcaag aaaaaaggcg taacagtcgt aacgaatgca atggcgaaag
    27721 aagcagtcga taacggtgac agcgtgacag tgaaatatgc agtggacggg aaagaagaat
    27781 ccgttactgc tgattatgtg atggtcactg taggtcgtcg tccgaataca gatgatatgg
    27841 gcttagaaca agcgggtgtc gaagtaggag aacgaggatt gatcacggtg gataaacaag
    27901 gacgtacgaa cgtacctaac atctatgcca tcggtgatat cgttccaggg gcggcattag
    27961 cacacaaagc cagttatgaa gcaaaaatcg ctgctgaagc aatctctggc aaaaaagtag
    28021 ctgtagatta taaagcaatg ccggcagtag ccttcactga tcctgaattg gcttctgtag
    28081 gtatgacgat caaagaagca aaagatgctg gtatcgaagc aaaagcatat aaattcccat
    28141 tttcagggaa cggtcgggca ttatcgcttg gtaaaacaga aggattcatc cgtctagtta
    28201 cgacgatcga agataacgtg cttattggcg cgcaaatcgg tggagtcggc gcaagtgata
    28261 tggtctctga attagcttta gcaatcgaat caggaatgaa tgctgaagat atcgccttga
    28321 cgattcatcc tcatccatct cttggcgaaa tcgtcatgga tgcttcggaa ttagcattag
    28381 gtttaccaat ccatatttga tttgaataat acaggcgaaa cgcaactgaa aaatcagttg
    28441 cgttttgttt tttagaatta tcaataatat tattatgtaa acttaaaaat atgacaactt
    28501 cttggtttat taccacttcc tatgctacac tgaagaggaa tatgaaatag gagagttgaa
    28561 gatgcaagaa tatcagtatc cacttgattt agattggaca acagacgaaa tggtcattgt
    28621 aatgaagatg tgggaagatc tggaacgtgc gaatgaaaaa gggataaaca atgaagcttt
    28681 tttgaagact tatcagcagt ttaaaactgt tgtcaaatcc attggtgagg aaaaacgatt
    28741 gggacgggaa tttgaaaaag cttccggtta ttctttatat cgtacagtgc agcaagcaaa
    28801 aaaaaatcca aacaaaactt tgagaatgaa cgggtgagat aaatggaaca gatgattgat
    28861 catatcaagt cctggcttga agaagctggt gagaaaatca aatcttctca acaaacatta
    28921 acaatctcgc aaaaatcaaa tcgaacggat ctagtaacaa atgtcgatcg aaacatccag
    28981 aattttctaa tcgaaaaaat ccattccttt gatccagagg cgaaaatatt ggcagaagaa
    29041 gaagggtgta atcaattatc ggatctcgat ggaagagtct ttatcatcga tcctattgat
    29101 ggcaccttga attttgtgat tgaaggggaa aatttttgta ttatgctcgc tgtttatgaa
    29161 tccggcgttg gaaagcttgg tttcatttat gacgtgatga gaaatgaact tttttggggt
    29221 ggaaaaggaa taggcgtgta taaaaatgat gagccattgc ctagaccagc aaataaatcc
    29281 ttatcggaag gcttacttgg tgtgaatagt tatcttttcg gtcataataa atatggaatc
    29341 cgagaaattg cggaaagcag catggggaca cgtatgtacg gatgcgctgg tcttgaacta
    29401 gtggcaatct tgaaaggaaa ccacattgct tatatttcaa accttagccc ttgggattat
    29461 gcagcgggaa atgtcttatt ggaagagttt ggtatggtaa caagcggtct ttttggtact
    29521 cctttgacat ttgaaaaaag ggaatactat ttagcaggga cttcttgtgc atatgcagaa
    29581 atcgcagacc ttttgaaaaa agaaggctga aaaaaaggta gaaatcattc acaaaaatga
    29641 taaaaacgag caaaagatta attttctcga aaaaatgaaa acattttact atatttcatg
    29701 aaaaaagtct gctataatta gcagtcgagt aaaatttgcc ctgtcataaa gaaccaataa
    29761 aaaaggagtt agttacattg aattatagaa acgatatccg taacgtcgca attattgccc
    29821 acgttgacca cggaaaaaca acattagttg atgaactatt aaaacaatca gatacattag
    29881 acgcacatac gcaattgcaa gaacgtgcga tggactcaaa tgcgctagaa aaagaacgtg
    29941 gtatcacgat tttagccaaa aacacagctg tagactataa aggtattcgc gtaaacatca
    30001 tggatactcc aggacacgcg gacttcggtg gcgaagtaga acggatcatg aaaatggttg
    30061 acggcgttgt gttggtcgta gatgcgtatg aaggaacaat gcctcaaaca cgttttgtat
    30121 tgaaaaaagc actggaacaa catatcacac caatcgttgt cgtaaacaag attgacaaac
    30181 cttctgctcg tcctgagcat gtcgtagatg aagttcttga actattcatc gaattaggag
    30241 cagatgatga tcagttagac tttcctgtga tttatgcatc tgcactaaat ggtacgtcaa
    30301 gcttgtcaga tgatccagca gatcaagaac caaccatggc acctattttt gatacaatta
    30361 ttgaaaaaat ccctgctcct gtcgataaca gcgacgaacc attacaattc caagtttcat
    30421 tacttgacta caacgattat gtaggtcgta tcggaatcgg acgtgtattt agaggaacaa
    30481 tcaaagtagg agaccaagta gcattaatta aattagacgg aacagttaag aaattccgtg
    30541 taacgaaatt atttggtttc tttggactaa aacgtctaga gattcaagaa gcaaaagctg
    30601 gagacttgat tgctgtctca ggtatggaag atatctttgt cggtgaaaca gttactcctg
    30661 tggatcatca agatgcgtta cctatccttc acattgacga accaacattg caaatgactt
    30721 tcttagttaa taattcacca tttgccggtc gagaaggtaa attcgtaacc gctcgtaaga
    30781 ttgaagaacg tttgatggca gaacttcaaa cagatgtatc cttacgagtt gaaccaacaa
    30841 actctccaga tgcatggaca gtttctggtc gtggagaatt gcatttgtct atcttgattg
    30901 aaaacatgcg ccgtgaggga tacgaattgc aagtatctcg tccagaagtt attgaaaaag
    30961 aaatcgacgg tgtaaaatgc gaaccgtttg aacgcgtgca aatcgataca ccagaagaat
    31021 atatgggtag tgttatcgag tccttgagct tacgtaaagg tgaaatgcaa gatatggtcc
    31081 atacaggaaa cggacaaatc cgtttgacat tcttaacacc tgcccgcggt ttgattggtt
    31141 attcaactga attcctttca atgacacgtg gttacggaat catgaaccat acttttgacc
    31201 aatatctgcc aatgcttcct ggtcaaattg gcggacgtca ccaaggggcg cttgtttcga
    31261 tcgatacagg aaaagcaacg acttattcta tcatgagtat cgaagaacgc ggtacagtct
    31321 tcgtagaacc aggtacagaa gtatatgaag gaatgattat cggggaaaac agccgtgata
    31381 atgatttgac tgtcaacatc acgaaagcaa aacaaatgac aaacgtacgg tctgctacta
    31441 aagaccaaac atctgtcatc aaaaaaccaa aacaattgac gttggaagaa tctttggaat
    31501 tcttgaatga tgatgaatat tgcgaagtaa caccagaaag cattcgcttg agaaaacaaa
    31561 ttttagagaa aaatgctcgt gaaaaagcat ctaaaaagaa aaaataatct gtaaaaggat
    31621 gaagagagaa accagcttgt tataaaaaat agctggtttc tttttttgtc caactctttt
    31681 ctgtggagaa gtcatctttg tcaatggaca taaattcaga aaatacgccg aaagacgttt
    31741 aaaagacttt taaaaaactt atttctagag agtttttgtt tcataataag ataatgtttc
    31801 acgaaaggag atcaacttgt ccttcgaaga aaatcgaagt aaaatggcaa gagaaaggag
    31861 cgggaagatg aacgacttat taaaacaata ctttggctat gatgagttta gacctggtca
    31921 aaaagaaatt atccagaaag tcattgatca ggaaaatgta ttaggaatca tgcctaccgg
    31981 aagtgggaaa tcaatctgtt accagcttcc agctttacta ttagatgggc ttactgtggt
    32041 tgtgtcccct ttaatctctt tgatgaagga tcaggtggac gcggcgaatc aattaggtat
    32101 acccgccact tttatcaata gctcgttaga tggttacgaa acagctagac gcttccaaga
    32161 aatcgataga cagcagtacc gtttacttta tatcgctcct gaacgtttta tcatgccgga
    32221 ttttatccaa gcaatgaagc gatggaacgt ccgtatgatc gctattgacg aggcacattg
    32281 tatctctcag tgggggcatg atttccgccc aagttattta cagatggcga accagctaga
    32341 tcaactgcca aatcgtcctg taattgttgc tttgacagct acagcgactg tgcaagtagc
    32401 tgctgacata aaaagacttt taaaaattcc agaaaacaac catattcaga ctggttttga
    32461 acgagaaaat ctacgatttc aagtaattaa agatcaaaaa aaagaacaat atctaatcga
    32521 atatttaaag atcaacaaaa atcaatctgg tatcatttat gcagcaacga gaaaagaagt
    32581 agaccgtctc tatcatttgc ttaaaaaatt cgatttttcc gtgggtagat atcatggtgg
    32641 tctaaatgag aatgagcgaa cagaaatgca agaagccttt ttatacgatc gcttgcagct
    32701 gattgtagcg acaaatgcat ttggtatggg tataaataaa agcaatgtcc gttttgttat
    32761 ccattatcaa attcctggct ctctagaagc atactatcag gaagctggtc gggcgggaag
    32821 agatggtctt tccagtgaag ccatcctgct tttctctcct caagatatcc aagtccaaaa
    32881 gttttttatc cagcagtccc aaagagaaga agggcaaaag caaaaagaat atgaaaaatt
    32941 aaaagcgatg acagaatacg tgtatattga gtcctgtctt cagcaataca tcctaaacta
    33001 ttttggcgag acaagcagtc cgtgcaaccg ttgtggtaac tgtttagatg acagagaaat
    33061 agtagaagta acgacagaag cacagatggt tctttcttgc ttaaaacgta tgggtgagaa
    33121 ctatggtaaa caaatgctga tgaaagtctt agcaggatcg aaagaacaaa agctgcaagc
    33181 tttaggtttt ggacatttat ctacctatgg actgatgaaa aaccaatcgc aaaaagaaac
    33241 gatgcagtta attgaatatt tgatatcgaa tggctatctt ctgaccatca atggtgagta
    33301 tcctgttttg aaagtaacag aaagagggat acaagtattg aaaggacaag aatctgttta
    33361 tcgaaaggaa cccaagaaag tccagcagct atcggatgaa gaaaccgata cactatttga
    33421 agtgctgcgg gaactgcgaa cggatctagc atcagaagca ggcgttcccc cgtatgtcgt
    33481 attttcagat tccacattga aagaaatgag ccgaatacgt ccatcaagca gattggagat
    33541 gcttcagata aaaggagttg gccaaagcaa gcttgataaa tatggcgaag cctttttatc
    33601 tcggataaaa aatgcggact ctaacgttct ggataagtag aaagaacgaa tacgtaaaaa
    33661 tgaataaaaa caactggtta aatctgtttt ttttgctcct tatgaaaatc aaatatgtta
    33721 taattaagga attagaatgg gaggtaagca cagatggatt ctttgtcgaa aaaacttgcg
    33781 atccaagtat taaatgatga agcagaacgg atcaaacgat taatccgaaa tcaaaacaac
    33841 agtctctgta tctcacaatg caaagccttc gaagaagtgg tagatacaca gatgtacggc
    33901 ttttcccggc aagtcggttt tgctgtccgt ataggagtaa tcggaaagaa cgagggccag
    33961 ctgatgttaa gtgagctaga acgagaattg aatcagttat atagtgaggt ttaccaagaa
    34021 atttatgata aaaaagaagt tggcaaggag gaatagactt gcctaagaaa gtgaaaaaaa
    34081 ggcatctatt agactatagc atcttgattc cctatttaat tttatgtgta ttgggattga
    34141 tcatggtata tagttctacg tcgtatctat tattagaaaa cggacaaaat ccgtccgcat
    34201 cagtcattaa ccagagtatt ttttgggtgc ttagcttgat tgctatcgca ctactttata
    34261 aaatgaaaac agatgttttg aaaaaccagc gtttgatcat ggcagccatc gcagttttga
    34321 cgattttgct gctgattgtt gtgttttttg gtaaggagat caatggggcg aaaggttggc
    34381 tgcaaattgc aggattctcg atccaacctg ccgaatacct aaaaatcatt tctatctggt
    34441 acctgtctct gactttatct aaaagacaaa acagtgtaca aaaagatttt cttgggactg
    34501 tgaaacgtcc acttgcaatg gttatcggac ttactgcatt agtcgctatt cttcccgatt
    34561 ttggtaatgc ggcagttatc tttttgatta ttttggttct gcttttagcc agcggagtca
    34621 attatgtgta tactttgatt gtaggagtag gaggcttttg tctaagtact tttacgatct
    34681 ggctgatcaa tatcacaaac ggaaagatct tacccggacg tttacagtat atctataatc
    34741 gatttgctat ttaccaaaat cctttctcag acgaattaaa taaagggcat caactagtca
    34801 acggctacta tgccatgttc aacggaggac tatttggcag gggattaggt aacagtatcc
    34861 agaaaaaagg atttttacaa gaagcacaaa ccgattttat ttatgcaatc gtagtggaag
    34921 aactgggagt gattatgggg attttgatat tagcgttgct attctttatg attgtgcgca
    34981 ttgtccttgt aggtatccgt tcaaaagatc cgttcaattc gcttttgtgc attgggatcg
    35041 gcgcaatgtt tttgatccaa gtatttgtca atcttggagg aattactggc gttattcctt
    35101 tgacagggat cacgttccca tttttaagtc aaggaggctc cagtttgctg atgctttcga
    35161 tttgtgtcgg ctttgtcctc aacatcagtg cggatgaaaa acgcaagagc ttaggtttat
    35221 aataaaaaaa gatgtaggag tggttcgatg aaaaaagttt tagttgctaa caggggtgaa
    35281 atcgctgtac gtgtatttcg tgcttgtaca gaattaggaa ttaaaactgt tggaatctat
    35341 gcagaagaag atgaatattc cgtacaccgt ttcaaagcgg atgaagcata tttagtagga
    35401 caaggaaaaa aaccgattga tgcgtatcta gatattgaag ggatcatttc tatcgcgaaa
    35461 gaatgcggag ctgacgctat tcatccgggt tacggtcttt tgtcggaaaa cttgaatttt
    35521 gcgcaacggt gcgaagaaga aggcatcatt tttgttggac ccaaactgca tcatttagat
    35581 atttttggag ataaaatcaa agcaaaagct gcagctatcg aagcggggat tgcttcgatt
    35641 ccaggcacag atggaccaat cgcttctata gacgatgcct tggagtttgc gaaacaatac
    35701 ggttatccga ttatgatcaa ggcagctcta ggcggcggcg gacgcggcat gcgtgtagct
    35761 cacgatgaaa agagtgcaag agagggctac gaacgtgcga aaagcgaagc aaaagcagcc
    35821 tttggtagtg atgaagtgta tgttgaaaaa tacatcgcta atcctaagca tatcgaagta
    35881 cagattctcg gggatacgca tggaaacgtc atccatcttt ttgaaagaga ttgttctgtt
    35941 cagcgccgtc accaaaaagt agtagaagta gcgccttgtg tctctatgaa tgaacagcaa
    36001 cgtcaaaaaa tctgccaggc agctgtccaa ttgatgaaat atgtcgggta tgtcaacgca
    36061 ggtacagtag aatttcttgt tgaaggagat gatttttact ttatcgaagt caaccctcgc
    36121 gtccaagtgg aacatacgat cacagaaatg attacagata ttgatatcgt aacaacgcaa
    36181 ctgttgattg ctcaaggttt ggatctgcat aaagaaatcg gattgcctca gcaagaagga
    36241 attaaattaa atggttcagc cattcaatgc cgaatcacta cagaagaccc gttgaataac
    36301 tttttaccag acacagggaa aatcgatacg tatcgttcac ctggtggttt tggcgtacgt
    36361 ctggatgtcg gaaatgctta cgcgggctat gtagtgacac cgtattttga ttcattgcta
    36421 gtaaaagtat gtacgcatgg tgctaccttt gaaacagcca ttcagaaaat ggaacgttgc
    36481 ttgaaagaat ttagaatccg tggagtgaaa acaaacattc catttatgct caatgtcatc
    36541 acacatcctg aattccaatc aggaaatgca aaaacaacct tcattgatag tacagcaaca
    36601 ttatttgaat ttcctcgact acgagaccgc ggaaacaaaa cgatgaaata tatcggtgaa
    36661 attactgtga atggttttcc aggtatcgaa agtggcgaaa aaccatttta tgaagaacca
    36721 cgtatgccta aagatctaat cacaagaact gattacgtaa cggctaagaa tgtattagat
    36781 gcgaagggtg ccgatgcttt ggtcgaatgg atcaaaggac aggaaaattt gttgttgacg
    36841 gatacgacat ttagagacgc ccatcaaagt ctgctagcaa ctcgtgttcg gacaaaggat
    36901 ttcaagcaga ttgcacgttt gactggagaa ggtcttcctg aactcttttc aagtgagatg
    36961 tgggggggag caacatttga tgtggcttac cgctttttga atgaagatcc ttggcaacgg
    37021 ttgagaaaaa ttcgttcttt aatgcctaat acgttgctgc aaatgctttt cagaggttca
    37081 aatgccgttg ggtattctaa ttatcctgac aatgtattgg tggaatttgt caaagaagca
    37141 gcagcgcaag gaatcgatgt atttcgaatc tttgatagtt tgaactggac tccacaaatg
    37201 gaaaaaagta tccaagctgt acgggatact ggtaaaatcg cagaggcagc tatttgctat
    37261 actggcgata tcaacgatcc aagtcgggca aaatacaatg ttcagtatta taaagacatg
    37321 gcaaaggaac tagaacaact gggtgcgcat atcattgcaa tcaaagatat ggctgggctg
    37381 cttaaaccgc aggcagctta tcgtttgatt agtgaattga aagaaacgac agatctgcca
    37441 atccatctgc atactcatga taccagcggg aatggaatca tcacttattc tgctgcttca
    37501 aaagcaggag tagacatcgt tgacgtggca atgagtgcaa tgagtgggaa tactagtcag
    37561 cctagcatga gcagcttgta ttatgcactg gtcaatggtc cacggctgcc agaaatcacg
    37621 attgaaaatg cacagaaatt aaaccattat tgggaagatg tgcgcatgta ttacaaaccg
    37681 tttgaaaacg gcttgaatgc tcctgaaaca gaagtatata tgcatgaaat gccaggagga
    37741 cagtattcga atctacagca acaggcaaaa gctgtcggat taggtcatcg ctgggatgaa
    37801 atcaaacaaa tgtatcatac tgtcaacttg atgtttggcg atatcgttaa agtaactcca
    37861 tcatctaaag tagttggaga tatggcacta tttatggtcc aaaatgatct tacagaagaa
    37921 gatatttatg aaaaaggcga aacactaagt ttccctgaat ctgttgtgac tttcttccaa
    37981 ggagaactag ggcaacccgt tggcggattt cctaaaaaat tgcaaaagat tattcttaaa
    38041 ggcagacctg cattgaatga acgtccaggt cttttggctg aatcagtcga cttcaacgaa
    38101 gtaaaaaaag aattggcaga aaaaattggc tatgaaccaa aacaagacga agtattaagc
    38161 tatttgatgt atccgcaagt ctttttggac tatcaaaaag catataatca atttggcgat
    38221 gttacattac tggacacacc aacatttttc caaggtatac gtttgggtga aacaatcaat
    38281 gtacaaatcg aacgcggaaa aacgcttatc attcgtttag atgaaatcgg agaacccgat
    38341 atcgaaggga atcgcgtact gttcttcaat ttgaatggac aaagacgtga aatcactatc
    38401 aaagataatt cgatcatcag tacggtccaa acccgattga aagctgaacc gacaaaccga
    38461 gaacaaattg gagcaacgat gtctggttct gttttggatg tattagtgaa aaaaggcgat
    38521 aacgttaaaa aaggtgatac gttgatgatt actgaagcga tgaaaatgga aacggccatc
    38581 gaagcacgtt ttgacggaga agtcgcacat gtatatgtgt cttcaggaga cactatttcg
    38641 tctggtgacc ttttgattga agtgactgag aaataataag cgaaaggtgg ggaaacatga
    38701 aaagactggg tttgttttgg ctgattctgc tattagttat tgtggcattc tacatggaac
    38761 cagtcttgtt tccgccaaaa cttgaagtga aggaaaactc tgatcaacaa atatcaaacc
    38821 aaatgacaac gtggaaatac caggagctta aagcaactgg tttttctact tatatcggaa
    38881 aaccgatcga tcagctggaa aaggattttg gccaagccta tgatcgatta actagcggat
    38941 ttggttttga aacaagatat tatgaaaata aagctagtca tctcacattt gaagcgaaca
    39001 tccaagacag cagagtcaca actgttaaag tactgcaaac agggcctgct gatatcgcgc
    39061 cgttttattt agggatgacg atgcaagatc ttacgaaaat cactactatc tatactaatt
    39121 tcacttttga gtaccatgat acagaagttg gaatcgaact aatggaagag gatatgaatt
    39181 atcgtccgct aattgctttt gataacgaca cttttgctat tttatttttt gatcaaacaa
    39241 caaacggcct attttcagtt gtgtatttag ataaagatag tcttttgaaa ctattgccat
    39301 atcaagtgtt tggtgaaggc cttccacatt atcaagcaga taaatcggct gattgggaat
    39361 caatcaatcg cgttaaagaa cgaaaaagta cgactttatt aaatatgctt agaagagaag
    39421 acgaacttcc catatacaat caaacacttg cctttcaaaa ccacagtcag ctgctactac
    39481 atgatttttt gacaaggcct gaggaaatat tgtcagagga acgtatggct gattggcaaa
    39541 aatcgctagg cagtgctcaa acgacagtga aattctcact gacgaatgat gaattggaga
    39601 agttaatcag taaacaaaaa ttagaaaata ctagtgggct atttattcat ccggcgattg
    39661 acgctacttt ttcgttttta tattggtaca gcgatcctta caaccatgac cgtttcatgg
    39721 acccgggaac ggattctgta ggcattgctt tttctaaaga aaatatgtta gtcttattgc
    39781 aagaagaaaa cggagaaagt agtgatagca gtgatcgtta acgaagagct gtttcaactg
    39841 gaggatcaat gcctccaagt cgctgaaatg ataaaaaaga gtaaaacgat ggcttcttat
    39901 cttgaacata aacagtcaat ggaagaatct gcaaaagttt gccagttgaa agaagcgttc
    39961 ttgtctaaga aagaagcatt tgaaaatgta gcggcatatg gtgagtatgc acctgattat
    40021 cgtgaaaaaa aacgagctgt tcgaaaagca aaaagacagc ttgatatgga tgagcatgtt
    40081 gcagctttcc gatatagtga aacacagctg caaagtctgt tggatcaaat tgtccaagta
    40141 cttgccgaag aagtttctaa tgaaatcaaa gtagatgcag gtagcccgtt ttttgaaacg
    40201 aaaaaacact ttggatgcgg aggtaattgt catgcaatta gacaatagtg aatttgaaat
    40261 aacaaaacga cgcgggctga tcgtatgggt ctacagctta aaacaattaa agaatctgaa
    40321 aaagttcggc ttgttgcatt atgtctcaag aagaatgaaa tatgtcgtgc tttacttaaa
    40381 tgaagagaca ttggaagaaa cagaagaacg aatccgtaaa ttacattttg ttcgaaacgt
    40441 agaacgttct taccgaccgg atatcgaaat gaattttgcc gaaaaaatag gaaacaaaac
    40501 agtattaaaa gacgaaggtt tcgaaattga agagctaaat acagaaatcc gattagctga
    40561 acatctttaa aaatctctta tgagcagttt ttaaataaaa aataacttgg ataacgatcg
    40621 atttttgatc attgtccaag tatttttttg gtatactagt accagtacca tttctaagga
    40681 gtgagtagat gcggataata tcaggaaatt atggcgggcg ccgtttaaaa agtttagcag
    40741 gggcgaacac cagaccaact acagataaag taaaagaatc catattcaat atgattggac
    40801 catattttga tggagagaca gtattggatc tgtttgcagg aagcggtggt ttagctatcg
    40861 aagcagtatc tagaggctgc agtcatgcgg tatgcgtaga caaaaattac caagcgctaa
    40921 aaatcatcaa agaaaacatc gaaattacga aagaaccaga gaaatttacc acaataaaac
    40981 ttgatgcgga caaagcaatc gaacggctag cagcggagaa acaacaattc gactacttgt
    41041 ttcttgatcc gccatatgca aaacaaaaaa taatcgatca aatccataaa atggaagaac
    41101 tgggactttt ttctaaaacc gcagttatcg tatgcgaaac ggataaaaca gtcgaattgc
    41161 cagaaactgt ggctggattc catcaaataa gaaaacagat ttatggcata acggccgtaa
    41221 ctatctatag aaaagaggga aatcaatgaa acgtgcatta ttcccaggaa gttttgatcc
    41281 ttttacaaaa ggtcacttag atacggtgga acgtgcggct aaattattcg atgaggtagt
    41341 aattggtgtt tttatcaata cttcaaagaa aagtttgttt cccccagaag aaagaatgac
    41401 attgatcaca aaagctgttt ctcatcttcc taatgtaaaa gtcatgcatc aagaaaatca
    41461 attgacggta gaaacagcaa aagagattgg tgcagatgcg ttgatcagag gcatacgaag
    41521 tatcaaagac tttgaatatg aacgagagat tgctcagatg aatcatcatc tatatgatga
    41581 attggaaact gtcttcttat tagcaaaacc agaatatagt cacgtgagtt ccagtatttt
    41641 aaaagaagta ctgcactttg gcggagatgt gagcagctac ttaccgccag tgatcaacga
    41701 agctttagct ggaaagcgtg aatcaaatga aagctaaaca atggttgaaa acagtactga
    41761 ttatcatatt tggaatgttg atcattggat ttgtagtagt tcccttgccg tattatgtag
    41821 aatcaccggg agccacgatc gatctgaaag aactaattac ggtcaatgat aaggaagata
    41881 aaggttctgg gtctttctct cttacctctg ttggtatcag gcaagctaca ggtttcttag
    41941 ctttaaaagc taaaatgacc ccttttgaag aattgatcag tgaagaagaa ttgactggtg
    42001 gggcgacaaa tgaagaatat aatcaaatgc aacagtatta tatggattct tcccaaaatg
    42061 cagcaatcga acaagcgtta aaacttgcag acaagcctta taaaatggaa ttcaaaggcg
    42121 tatatgtcat gagtgtagag aaaacatcga atttctttgg caagctttca gttggagata
    42181 cagtaacaaa agtagacggg aaaagctttc aaagtacaga agcatttatg gattatgtaa
    42241 aaagccaaaa agttggacaa acaatcgaaa tcagttattt gcataatggt gaagaaaaaa
    42301 cagcttctgg tgatctgatc gagttaccaa cagataaaaa ggcaggaata ggtatcacgc
    42361 ttactgacca taccgagatc gaatcagata cagatgttcg tattaattct ggctctatcg
    42421 gtgggccatc agcgggattg atgttcactt tagaaatcta tgaacaagtc actggtaaaa
    42481 acttgcgaca tggtaagcaa attgcaggaa ctggaacgat caacagtgaa ggagaagttg
    42541 gccgaatcgg aggaatcgat aaaaaagtgg caagtgcgga taaagctggc gtagagattt
    42601 tctttgcacc agatgatacg atttcagacg atgttaagaa agaatatcct gaaataaaaa
    42661 ccaactacga agaagcaaaa gctgccgcta aaaaattgaa ttcctcaatg aaaatcgtac
    42721 cagtaaaaac agtacaagaa gcgcttgact atctagagaa aatgaaatga aactaaataa
    42781 ttaaagaggg gttgtgacac gagttctgtc acaacccctc tttttcgtca aaaatcttca
    42841 cgataaaaat aaaaacaaaa gaaaaaattg acgtaattag atgttgaagg gacgattttg
    42901 ccagagctca ctgtttcttc aattttaaat gaaaggagta atgaatgatt tttactggtc
    42961 attctttgaa aaaagaacaa tgatagaaaa aatcagcaag aaaatactag cttcacccaa
    43021 attgatcatt tggatgtcta tgatagttct tatcttttgt atcgcaagtg gttttttatt
    43081 atcaaatttg ctattttata gagaaacaga agagactgct gcaaaagaaa tactttcttc
    43141 gtttagttcc agtggagaag atccatcagc tgaaggaaac gatgtagaag aggatgctcg
    43201 tccaaaggtc atgtacaccg atatcaaagg aagtgtgaag gagcctggta tctattcctt
    43261 ttcttcagaa gaacgggtct atgatgtact aaagcgtgca ggtggattat tagaagaagc
    43321 ggatagcgat cgaatcaact tttctgcgaa gatagaagat cagcaagtcc tatatatccc
    43381 tgctgtaggt gaagagctgc cagaacatct tgatcagttg gcatcctcgg aaggaaaaca
    43441 gcctacagca gattccgagc ctcccaaaat aaatatcaat acagccactc catcagaact
    43501 tcagcagatt cctgggatcg gatcagtcaa agcgcaagaa atcattcgat ttagagaaga
    43561 aaacggatcc ttccagaaag tggaagacct gcaggagatt tctggtatcg gtgaaaagac
    43621 agttgaaaaa ctaaaaaact tcgttacaat taaatagaat gattcaaaat cgactggcac
    43681 gagcaataaa tggacgctaa tggatactaa gaataatcca attctaagag cttggcagga
    43741 cgaggaggta gtatttttgg ataggaaata gcttgattgt tcgcaaagaa acattaaaaa
    43801 aaccaatgct gaatgtctat ctccaaaata aaatatctgg gattcatatt atgaatacgg
    43861 ctgtcagcgg caataacagt caagctctac gagaacgctt tgccaaagat gtacttagct
    43921 atacagcaga taaagtattt attctcattg ggacgaatga tctagcagag cataaacaac
    43981 tttctaaaga aacgtaccaa aaaatctgca gtggatgata gttcagctga aaaaagacta
    44041 tagttctgat catgctttct ttatcacgcc accagcagtc gatgagacaa aacaaaaata
    44101 tcggaataac aagttgatca aagaatatat ctctgttctc acagaagtag caaaacgcca
    44161 tgattgtgaa gtatcgattt ttatacggaa tttcttgaac aaatggattc gaagaatttt
    44221 tataatgggc taatggatga cggacttcat ttcggcagtg aagggtagga tcttttgtca
    44281 gacaggatga ttcgttatat ttaaagaata ttttgtttaa gcaatgagta ctttttaatc
    44341 aacttgaaaa aacggaggaa tataaaacat gttagaaaaa tcttcaaaag catgcgatac
    44401 gaataaagaa aaagttactg ataagtcaca taatgaacgc attccttggg accagtactt
    44461 tatggctcag gcagtactgt tatctttacg tagtacgtgt acacgtttag aagtaggagc
    44521 gacactcgtt cgagaaaaaa ggatcattgc tggcggctat aatggagcag tcagtggaga
    44581 tgtccactgc atagatgaag ggtgttacgt cgtggatgga cactgcttgc gaacgatcca
    44641 tgcagagatg aatgcactcc tacagtgtgc aaaacttggt atcccgacgg aaggttcaga
    44701 aatctatgtc acacattttc catgtttggc ctgtacaaag gctttgttgc aagctggtgt
    44761 gaaagggatc aattatctcc atgactatcg aaatgatcct tatgcccaag cgttgatcga
    44821 acaattaggt gtatctgtac accaagttgc tttagactca cagcattttt caaaacttca
    44881 aaatgaatta acaggaaaac atgtctagcg ttttagaaag gcatgttcga aatcggttga
    44941 tttttccggc aattacgtcg atgattatct gtttgttgta ttacactgac aattggggtt
    45001 atcgtttgct tcttgccgga atttttttat ggttttcgct aaaagaagga cgttttatcg
    45061 cgtacttgtg tattttgtgt agcataacgg tatccatttc ttataggcta caagagtatt
    45121 catttttaga ggaaaaaaca acatctgcgc aaattactgt atttacagat acgattcgtg
    45181 caaatggtaa ttttttgaca atggaaggaa ggttatcaga gaataaacaa aagctccagc
    45241 tttcttatca agtaaacaca gaagcagaat tgaaaacatg gcttaacttc cgaggaaagg
    45301 gaatagaatt gactgctgaa ggcgagttca tctctccagc aaaaaagcgg aatctttata
    45361 attttgacca agaagcttac ttcaggaacc atagaatttc tggacggttc caaattcaaa
    45421 agattgaaca gatcaccatg caaaatcact ggcgaaactg gcttcagaga aaaagaggat
    45481 cttttatctc ctttgtccaa tctgaatttc cgcaaaaaac aagtgtatat attaattctt
    45541 tattatgggg ctataaagat gcaggattca gagaaccaga agaaattttt agggagagtg
    45601 gtttgctgca tctgttcagt ctatcgggtc tacacatcca attttattta gggtggctgt
    45661 attatttatt tcgacgtatc ggatggccac tacacgtcag tattattccg ttgagtctac
    45721 taactatctg ttatgtttct attgcaggaa gttctatttc tgttttgcgt gctagtttgc
    45781 tgtttttatt gcgattgcta gtcaaattgc tgaataagca ttattccacc atggacttat
    45841 ttgcagttgt cttttggctt cttttactat ttgatcctcg atttcttttt catgcgggtg
    45901 gacagcttag ttttttcatg agttttttgt ttatcctgat ttcttttgat ggagattggt
    45961 tgaaaaaaac ggcctctcac gtcctgttta ctatgatcac gatgccgttg attgtatggc
    46021 atttttatga gtggcctatt ttaggtagtc tttttacatt actgattgca ccggtattca
    46081 agtttctttt tcttcctggt gtgttatttc tcacactctt caatcgctgg ctgccggaaa
    46141 ctttactttt gctgatagaa gagggattaa tcttatttga gcgaataatc gatttttctt
    46201 cagggtcgtc tctcattata ggacgtttgc cccttgtgct atctgggatg tatattttcg
    46261 gattactgat ttgtatggat aggttgaaag aacaacctgt gaaacacttg ttttatgttg
    46321 gtcttttttc ttttattttt ttgactttgc catttttacg cttttcttca acgatcgctt
    46381 ttatcgatgt cggtcaagga gacagtatct ttttgcaaag tccttttcga aaagaaacga
    46441 ttttgatcga tactggaggt cagttacact ttgaaagaga aaagtgggcg caaggtatga
    46501 tacgtcctcc tgctgaaaga aatatcgttc catatctaaa aggtcagggg ataagtgtaa
    46561 tcgataaact catcttgaca cacgacgata cggatcatgt gggagaatta cccacgttat
    46621 ctcagcattt tactatcaaa aaaatttata tcggttgggg cgctggacga aatgagccat
    46681 tacaaaggca tttatctgaa gctcaaaaaa atggtactga gatcattgaa atcaaacatg
    46741 gggacaggat cagcggttat tatgatcttt atgtattgac tccttttgaa aagggagagg
    46801 gaagaaatga agattcgatt ggtctatgga tggaatataa caataggcgc tttttatttt
    46861 taggagattt gaatcaagca atggaaaaac aattgctttt gatttatcct aatctgaaag
    46921 ccgatgtagt caaattaggt catcacggta gccgtacgtc aagtaacaca gattttcttt
    46981 cccatattga agcaaaacat gggattatat cctgcggcgt aaataatcgt tatggtcacc
    47041 cgcacagcga agtattggag acactagaag aaaatcagat aaaaacatgg cgaacggatc
    47101 aacaaggaat gatcacctat cgctggcatc cagtttttca tcctcatggg ctagtggaaa
    47161 caatgataga ctagtaattc tcatctgctc atggtaggat aatttttgaa agtggtgagt
    47221 aatttgagta tacaagcaga gttacaaaaa atacgtaaag aaaaacttgc atcttgttac
    47281 ttgattttag gaacagaaaa gtttctccaa gatcaagttc ggacagaaat tgtcaaaaaa
    47341 atccaattag agggcgaaga tgacttaaat tttttaagtt ttgacatgga aaatgcaagt
    47401 ttagatgagg tagtagctga agcagagacg ttgccgtttt tcggagatca acggttagta
    47461 ttcgtagaga atccttattt tttaacaggt gaaaaagtaa acaatggaat cgaacaaaat
    47521 actgatttat tgacggctta tttgaaagaa ccattagaat cgactgtatt agtattcttt
    47581 gcgtcatatg aaaagttgga tgaacggaaa aaagtaacga aacaattgaa aaaaacagca
    47641 attatcatca atgtccaaca gttgaatgaa aaagaagtac gtcaatatct gatgaatacc
    47701 ttagagaatt cctcaatcaa aatggaacga agtgcaattg atttattctt gaggttgacc
    47761 gacttagatt tatccaaaat gatgagagag ttacagaaat tggtccttta tggacaaaat
    47821 caacagcaaa tcacggtaag agaagtagaa gaattagttc ctaaaacgtt agaacataat
    47881 atttttgaca tgacccaata tattttaaaa gggaagacgg aacaagcttt gcgtttatac
    47941 gaagatttag ttatgcaagg ggaagaaaca atcaaaatta atgcgatttt gctttctcaa
    48001 ttacgtttat ttttacagac aaaatttctt gtaaagattg gttatcagca ggcaaatatt
    48061 gctgaaacat tgaaaatcca tccatatcgt gtaaaacttg ccatgcagga agtaaaaaaa
    48121 ttcgatgaac atttgctggt acagttgttt gatcaattag ttgagatgga ttatcaaatc
    48181 aaaacgggac aaatcgaaaa agaattaagt tttcaactat tcgttttgcg cacagggcag
    48241 ttgttaagaa atcgataaga agaaagacga gggcaaagca tgcgggaagg tcgtttgttt
    48301 ttgtcctttt tttgacaaat aaaaaaatcg gccgaagccg attcttttat ttagcgattt
    48361 ttttgcttaa acgagatttg tcgcggttag ctttgttttt gtggattaga ccttttgatt
    48421 cagccatatc aatagcttta acagcttctt tatataacgc atctacgttg tcagcacctg
    48481 aagctacagc atcttcaaat tttttgattg cagtacgcat agcgcttgtt tgagatgaat
    48541 ttttcacatt agcgttttcg cttgtacgaa cacgtttgat tgcagattca atgtttggca
    48601 ttagtttcac ctccgataac ttaagttctt acgcgtgtta ccgcataatt caacattcat
    48661 tattatacct aaaggcagcg tgctttgcaa tatgaacatg acaagttttt ttccttaaca
    48721 gtcttttatt aggatttttt ttcagtaaaa acggatagta caagctgaaa aaaatcaaaa
    48781 agacgctatt gtgggaggca atagcgtcaa accaaactag atttttattt atctatttaa
    48841 acaaagtttg aatgggaatg tttggtacta agttattata taacaaagag aaccgtttgc
    48901 aaagtttttt ttgaaaaggg atacgattcc ctgttgttct ttgtttttct attaagaaac
    48961 tgcagttatt ttataaccag tatcctttga tcagtaaagg ctattctggt gtaccgatta
    49021 ttttaatgta ttcatcgata ttttttatca acattttttt tatgaccaac tggttccgac
    49081 ggttccattt gccacttact tccagcacat atttatttag gggcaaacgt aagatatatt
    49141 ttgccaaata gcgatcttta agcaggccat tgatcacacg gtcctcaagc tgcaagttga
    49201 tcccagtcag catcattggc tgttcttgta aaacgtacgt atcaattaaa agaccttcaa
    49261 aagtttccac aagcgtcttc ctttcttttc aaagtaacct gaaaacaggc actaattctt
    49321 ccattttgat attcgttgaa gtggatgcga tcattctttc ttttgtccac tgtaatctag
    49381 tataacaaaa aagagcagaa gagccaaaaa gaaaaaagag catcgaattc tataggaagt
    49441 ctcattggca cggcctaaat tattacaaaa cattgattaa aaaaagcccc tgcatcgcag
    49501 gagcatcccc tatatcgttt taataggtca atcaaaaagc agtatcaaac acattttgta
    49561 tcaaatcagg aaataataga taagctaagc ccagaatcaa aagtaccgct agaagaaacc
    49621 agccgattgt tcctatgaaa tactttaatt tttcgaacat tgtatcacct cgttgtcagt
    49681 atatcacgga tcagtaaatt gggggtgtta agaatctcct aaaatagaca aaatttgttc
    49741 tattttccta aagcaaatac ttgtaagata cagtaagtga taccagaagc aatgaccggt
    49801 ccggcggcga ttcctcttaa gaagacaaca cctaaaatcg tgccgaaaac aagtgcaaca
    49861 gtgatttgtg gatcagaagc aatcaatcct acacccttgg aagataagac agccactagc
    49921 ccaccgcaaa aaacagctac ccagccaata ggggatttaa aggcttctaa tagatcacgt
    49981 agaccgattt ctcctgttgc gatagggatt agaatagccg ctgtgatgat cgtcacccca
    50041 atattgattc ctttgccttg aagccaaggg aaaaaggaac gggaggctgg tatgagcttc
    50101 ataatcaaaa caaaagctgc cgctatcatc agactttgat ttttagctaa aattgctaca
    50161 agtaaaataa ttcctaaaaa aagccaactt tccatgaaat atattcctca ctttcttcga
    50221 aacaatcata acatgttttt agaaagttac aaaagtagga attcatcctt caagacctat
    50281 tcataagctg tttttttcat aaaataaaca aaatcataaa aaaacacttt gcttatttga
    50341 cagaagacag ccgcgctttg tatagtggac aagacttaca tttttttgaa gaatcagaaa
    50401 gatcaggtaa aggtcttagt aaacgcaagc cattcagaat aaccaatatt gtgcttcctt
    50461 cgtgtcctac aactccaaga ggtagattta aaaattgcag gaagttagaa gcaatcaaca
    50521 agagaataac aagaatcgaa aaaataatat tttgtttgat gatccgccgc atttttaaag
    50581 atagccgatg actgtagaca aatttattta agtcgctttt cattaagacg atgtctgcga
    50641 tatccatagc ggcatctgtc ccttgcccca tagcgacacc taaagaagcg gctgctaaag
    50701 caggcgcatc attgactcca tcaccaacca tcgcatttac ttggtaaagt tcattctctt
    50761 ttttgactag ttgtgttttt tcttccggag tacagcttgc atagtaatgt tcaatcccta
    50821 cttgttcagc aatggccttt gctgtgccag aatggtcgcc ggttatcata cttgtatgga
    50881 tgttttggga tttaaagtag gaaattactt gttgggtatt tgcttttgga atatctagca
    50941 gacctaaaac agcaattaac tgctgatctt tagaaagata aatcacggtt ttgccttgat
    51001 tttccaaacg ttcgatcatt tccgcaatct ctttgctgat tatcatcgtc tcaggatcat
    51061 atgcgtgctt gccaactttc cattgcgcac ctaaataggt agtttgtaat ccaaaaccag
    51121 taatttcttc tattggtaag tattggattt cttcaggtat tgtgcaatcg aaatattgca
    51181 aaatcgcatg agcaagtgga tgcgtcgttt tttgttccat agcaacaaga agttgttgtg
    51241 ccaaatcctt ttcatcaaag aaaaagctgt cagttacaac tggttttcct ttagtgagcg
    51301 ttcctgtttt atcaaaggca atcgctttta attcagaaag cttttctaaa tggataccgc
    51361 ctttgattaa aatcccattt tttgcagaat ttgaaagagc agctagagaa gctggtgtag
    51421 cagatgcgac aagcgcacaa ggagaagcca caactaataa taccattcct cgataaaagc
    51481 tttcatccca actccaacct aggaaaaagt gggggagaag gatcattaga ggaacagcaa
    51541 gcaaaatcaa ttttacataa ttattttcga ttcgttcgat aaatgaagct gtttgagttg
    51601 gcgtattctg cgcttcttct actaattgga taatcttagc aaagacagtt tcggaactcg
    51661 tttttgttac tcgaatcgtg atgggatttc cttgattcag cgttcctgca tacatctcat
    51721 cgcctaaata tttttctaca ggcacagatt caccgcttaa tgcagactca tcgatcgttg
    51781 cctgtgtgct ttctaaaaag ccatctatcg gaacagctgc tcctttagcg acaaatacca
    51841 agttatccac ctttagttga gaaacatcta cttcttgcat ttgtccattt tcttttaata
    51901 attgggcttt ttgtggttga agattcatca aagcagtaat ttctttttga cttttattag
    51961 ttgtgtattc ctctaatgca ccgcttaaac aaaagatgaa tgtcaacatt gctccttcaa
    52021 agtaattacc gatcaaacag gcaccaatag cagctaaagc catcaataaa tccacattca
    52081 gtgtccgatc attccacaat tctttcaagc cttccgatgt ttgtttatat ccaccgatga
    52141 taatggcact gatataaaaa agtggggtga tggtaagaga gaatcggtcg aagataaaac
    52201 cgaagatcat aaataaaagg cagccgattg ttgctgcaaa agcttttttc tgattagtca
    52261 tcgttattag acctccttgt caaaacaagg atagcataat aaaaatgtaa ttgataataa
    52321 taacagctaa atgagaacga taatggttat catttggtga ttaagcttga tgttgttttt
    52381 attatttata atgattatta ttaaagaaaa taaaaaacca gaacatttct caaataagag
    52441 aagtgttctg gttttttcta cattgcaata aaccaataac caatcaaaat gataccaagg
    52501 ataatacgat accagccaaa agcggtaaag tcgtttttct tcaaatagct tagtaagaat
    52561 ttgatagcta agacggaaac cacaaaagat acgaatgttc ctacaagaag aacaacagtt
    52621 tcttccattt tgaatgaatt cccttcagct aaatatttga cgatcttcaa gccgcttgcg
    52681 ccaaacatga caggaatccc taagaaaaat gaaaactctg tagcgacaaa acgcgaagca
    52741 ccaatcaaga tagatcctag gatcgttgcg ccagaacggg atgttccggg aacaagtgcc
    52801 aagacttgga ataacccaac aagaagagct gcttggtacg taaagtcatt cagagaagtc
    52861 caacgaggtg ttttgttttt gttgcgtttt tcgacaataa taaaggcaat accgtaaacg
    52921 atcaaaacaa tggcaactgg taagaacttg tggaaatttg cttctaacca atcatccaaa
    52981 ggcaaaccaa tgatgatggc tggtatgcaa gcaacgatca ctttaaacca aagtgtccac
    53041 gtatcttttt tttctaccgt tgatttactt ggagaaaatg ggtttaactt gtgaaaataa
    53101 agaacgacga cagccaagat cgcccctaat tggataacga cattgaacat cgacataaat
    53161 tgtgaactgg catccagttt gatgaattca tctgctagga tcaaatgacc agtactactg
    53221 atgggcagcc attccgttat tccttcaata ataccgagaa tcactgcttt gataatattt
    53281 gcaaaaaaca taaattcctt ccttcccaat taagatacat cttatagtat accgttattt
    53341 tttgagaaac ccaatctgtt ttttaatatt aagaaaaact tgagaaataa agcagataag
    53401 tacacgaaga gaaaaaagaa gacaaagccc tcgttcagac tttgcctttt atgattctct
    53461 ggtaaaaatt aaaatatccc cattatagaa acgagtcgcg actatgtgtt ttcccgttat
    53521 cgatataaga cgtatcattg atgccgtgga tagaaaatcg accgtttgca taagtgacct
    53581 ttgttacgct gccatttaaa agcccagcac gaacaggctg tttaggatcg atcagattaa
    53641 gcaaaaaggc gatcgtcaaa ccatgagata cgactaaaac atttccacca ccattttttt
    53701 cgcagtgatg ggcgatatct tcaaatcctg tccatacacg gtctcgcaat gtttcataag
    53761 tttccgccca cccagcggta tctaagttgt atacagcatt agcaatttgt tcaaaagaaa
    53821 cttctgtggt aaacatttca tcatatgttt taaaattcaa tacacgaggg atcacacccc
    53881 acatttccat gtcatatcca ccttcaagag acccgaaaca ccattctcta atcctttttt
    53941 caataaaata aggaatttct tttcccttag aatgttctcc tagaataata cgggcagttt
    54001 cgattgcacg accactgtca ctagaataag caaatgaaaa gtcgacattt tttaacccgc
    54061 gacctaaata atgaatgcct tcttgtcctt gctttgtgag gggtgtatca caccagcctt
    54121 gtactcgttc aattgtattg aacatcgttt ttccgtgtcg tacgatataa agtgtagttt
    54181 ctgtcatttt attctcctcc taagtcgttt taacgattga aaaaaggatt tgtccttttt
    54241 tcctgttcaa tcgtcgttgc atcgccatgt cctggataga ctgcaaattc atcaggtaat
    54301 acaaacagct gggtccgaat actatgaagt aattgttcta aattccctgt atacagatcg
    54361 gttctaccga tacttcctct aaacagggcg tcgccagaaa cgacaaaatc atcaaaaata
    54421 aagctgacgc ttcctataga atgccctggg gtagggacga cgcgaaaaga catgtttcct
    54481 aaagtataag ttttcatttc aaattcatat tctgcaggcg agacaataac atcagcaata
    54541 tcatcatgtc taccgagtcc agaaagattc aaaatcggat cacctagcca tttttgctct
    54601 agtgggctga cataaactgg gatttgataa tacgcgcgta aaggttccac tgcaccgata
    54661 tgatcgtagt gagtatgggt aagcaaaatc gctaccggct gttggtttgt ttttttgatt
    54721 tcttgtttga ttcgttccgc atcttctcca gggtcaatga tcaaaagtgc ttcttcgttg
    54781 tagaccaaat aacagttttc ttcaatcatt cctgttttta atctttcgat atgcatgtgc
    54841 attcctcctt gatagccaaa acttctttca tccattagta taacgcattc attgctagga
    54901 aacaaaaaca ggtacttatt tctctttact tatctgaatt atcagactat atgaaaagta
    54961 tttggatatt ctaaagagaa ggaaagtaag ttttcatagc attttaatat gtccttgtta
    55021 cattagattc atccaaaaat ccaaattcta tatcctatat ccctgcctaa tcagcagggt
    55081 gtttttttat gtttggaaag agagctcttt ttgctaaact aggttttttc aggtaatctt
    55141 agagagaaag aggtggtgga aatgatcaaa cagtcaaaaa tatctgtgcg gcacttagta
    55201 gaatttattt tgcgacgtgg aagtatcgac aatcgtaaaa aaagtaacca tactgcatta
    55261 gaaggagcga gaatccaccg caagctccaa aaagatgcgg gagaaaacta tgaaaaagaa
    55321 gtctttttaa aaacaacagt tcaattagat gaatattacc tgatcgttga aggccgtgca
    55381 gacggtattt tcaaaagaga cggtatttat tatatagatg aaatcaaaac ctccgaacca
    55441 cgatttgaag atttagaacc agaacaagtg gaacttttct ttcatcaagc aagggtgtat
    55501 gcctatatct atagtcaaga acatgattta caagaaataa atctgcaatt gacttacttc
    55561 cagacggcag aagaagtgat cacgagaaag gttgaacatc agacaagaga gcagttagaa
    55621 acttttttta aaaagctgac tgaagattat aaagaatggc ttgtttttca agaaaactgg
    55681 cgcactgtac gcaatgcctc tttgatggct ttgaaattcc cccatgatga gtataggaaa
    55741 ggtcagcgtg aactagcggt agctgcttat aaaacgatcc gtacgaaaca gaaattgttt
    55801 gtagaagcac ctaccggaac aggaaaaaca atctctacgt tattccccgc tttgaaagct
    55861 gtaggggaag aagagggaga aaagatcttt tatctaacgg ctaaaacaat aactagacaa
    55921 gtagctgaag atgctatgac agcattaaaa gatacaggag ctgaagtaaa aagtgtcact
    55981 ttgacggcga aagacaagat ctgcttttta gatgagacga cctgtaatcc agatcagtgc
    56041 ccatatgcca atggttatta caatcggatt aatgaaggtt tatgggatct tttgaatcat
    56101 gagaatcaga tcactcgtga agtcatcgaa acttatgcaa gaaagcatac actttgtcct
    56161 ttcgaactat cattagatgt cagtatctgg tgtgacgtaa tcattgggga ttacaattat
    56221 ttatttgatc caactgttta tttaagacgt ttttttgaag atgaaaaaaa cgaggactat
    56281 ctgtttttga ttgacgaagc acataatttg gtgaatcgtt cccgagagat gtattcggca
    56341 gaactatctt atgaaaaaac gaaacgaagt aaggaagcta taccaaagga attcaaaaaa
    56401 cttcatcgcc gtttcaacaa actattaaaa gaatttgatt cgatccgaga aatagcaaaa
    56461 gaagatcatt gggattatca tcatcaaaaa gcaccggctg aatctttagt aaaggcaggt
    56521 tatcaattaa gcgaaaaaat taaagaatgg ctggcagaat ttcctgagca cccatcacaa
    56581 gaacaattgc ttccttatta ttttgatttg cttcattttt tgaaggtcag tgaattttat
    56641 gatgatcact acgaaacaac agtcgaaaaa acgtatcgcg atctgatagt aaaagagttt
    56701 tgtattgatc caagcttgtt tcttgagcag tcgttggata aaggaaaaag cagtattctt
    56761 ttttctgcta gtttttcacc actctcttat tatcaggaaa cactgggggg acaaaaaagt
    56821 ctggcttaca aactgccgag tcctttccca gaaggaaacc agcaagtttt catcgcaaat
    56881 tacctagaga cgacttatcg aaaaagggaa cacagtctgg caaaattagt agagacgatt
    56941 catgcttttg ctgatggaaa ggtcgggaat tatttcgttt tctttccttc ttatcaatat
    57001 ttagatcttg cggtggaagc attcagacag caatatcccg acagcaatgt gctaatccaa
    57061 gaaacggaca tgaatgagga agaacgcgaa cgatttttaa caaaatttca aacaaatccc
    57121 gctgaaacat tgattggatt ttgcgtactt ggcgggattt tttcagaagg aatcgatttg
    57181 agaggggacc gattgatcgg cagtatgatc gtaggtgtag gtttgccgca gatgaatcat
    57241 gagcaggaac tgatcaaatc atattatgat gagaaggaac accttggatt tgcttacgct
    57301 tatcagcttc ctggaatgaa caaagtcttg caagctggtg gtcgagtgat tcgtggcatg
    57361 gcggatcaag gaatcgttgt attggcagat caacgatttt ctagtttttc ttatcgacgt
    57421 ttattcccac aacattggca aacagcaaga gaagtacgaa gtgttgggga attggaagaa
    57481 ggaatacagc gtttttggtt atcaaaagaa gcagtaaatc aaaaaaacga ggaatgacag
    57541 acgtcagcct cgttttttga ttaacgatag ttagtgaatt gcaactcgat tggtaagtca
    57601 agtttacgta ataaagcaat cacagcttgt agatcatcac ggttcttacc tgtcactcta
    57661 atctgatctt cttggatttg tgcttttact ttcagttttt catttttgat agctgtagtg
    57721 attttcttcg cgttttcacg atcgattccg ttgaccaaat cggcagtttg tcgagctttg
    57781 ccacctagag cgtgttctga ttcggaaaaa tggatatttt tgattggcac atttcttttg
    57841 atcagcttac caaataatac atctttgatc tgttcgattt tgaaatcatc atcgctcaat
    57901 acaactagat tgctgttctc taatttgatt tctactgtag aatccttgaa gtcaaagcga
    57961 ttggacaatt cttttgttgt ttgctgaatc gcatttttta cttcttctgt attgacttct
    58021 gatacaatgt cgaagctagc atcttttgcc ataggaaccc tcctgatata catataaata
    58081 tttaccatac atctatctta tcataggatt agggcagatt cttacctgtt tgctaaaatt
    58141 cttctcattt gagttcgaag tattttattt taatagataa actgttattc ttacatttgg
    58201 aataccaaag taggggagag gtgcttgtga gtaaaattat gactgcgtat cataaaaatg
    58261 aaagattaaa gaaaatagtg gttattttga taaccggatt gaccgctgcg gtcgctttaa
    58321 attatttttt gattccagcc aatgtctttt ctgcagggat gaatgggatc gctcaaatca
    58381 tttcctcgct tttatatgaa tggtttcata tcaagataga cacaggaaca tttatttttc
    58441 ttttgaatat ccctgtgttt gtattagggt ttctcaaagt aggaaaacga gcaacgatcc
    58501 ttagttttgt gaatgttgta tgtgtatcgg tgatgacgat gctgcttcca gtcggacaga
    58561 tcactgataa tattttgatg aatgccctag taggcggtgt tttattaggt cttggcgtag
    58621 gattatcttt aaaaatgggc tttacaactg gtgggatgga tatcatctct cttgtacttt
    58681 cacaaacaac tggaaaaaca gtcggaaact acatgctgat tctaaatggt atcattgtag
    58741 tcatcgcagg cttcttcttt aactgggaaa gtgccttgta tacgattatt tccatttatg
    58801 caatgactca agtaattgat gccatccaca cgagtcatca aaaagtaaca gcaatgatcg
    58861 ttaccgtcaa gccagaagag gtaacagaag aactagcaaa acggatggta cgtggaatga
    58921 cattactgcc gtctgtcggt ggctattcaa aaaaacaagg gaacatgatc atgatggtca
    58981 tcacccgcta cgaaatgtat gatcttgatc agatcgttca tgaaatcgac gacgatgcat
    59041 ttatcaatat tttaccaact cattcagtat ttggccggtt tgcaaatgaa aacgaacaaa
    59101 aaatgtatcg aacaactggc gtattgccag aaataaaaaa tcgttcacaa aaaaaatgag
    59161 gaatcaggtt tattttagaa
//
LOCUS       DAFCNS010000007        56177 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFCNS010000007 DAFCNS010000000
VERSION     DAFCNS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.007"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(81..1637)
                     /gene="rny"
                     /locus_tag="LNE23_000423"
     CDS             complement(81..1637)
                     /gene="rny"
                     /locus_tag="LNE23_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBK6930301.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(2048..3097)
                     /gene="recA"
                     /locus_tag="LNE23_000424"
     CDS             complement(2048..3097)
                     /gene="recA"
                     /locus_tag="LNE23_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBK6930302.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3206..4447)
                     /locus_tag="LNE23_000425"
     CDS             complement(3206..4447)
                     /locus_tag="LNE23_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBK6930303.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4687..5265)
                     /gene="pgsA"
                     /locus_tag="LNE23_000426"
     CDS             complement(4687..5265)
                     /gene="pgsA"
                     /locus_tag="LNE23_000426"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBK6930304.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5474..6397)
                     /locus_tag="LNE23_000427"
     CDS             complement(5474..6397)
                     /locus_tag="LNE23_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930305.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(7136..8422)
                     /locus_tag="LNE23_000428"
     CDS             complement(7136..8422)
                     /locus_tag="LNE23_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK6930306.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(8415..9683)
                     /locus_tag="LNE23_000429"
     CDS             complement(8415..9683)
                     /locus_tag="LNE23_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK6930307.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            9935..10396
                     /gene="mscL"
                     /locus_tag="LNE23_000430"
     CDS             9935..10396
                     /gene="mscL"
                     /locus_tag="LNE23_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBK6930308.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(10455..10901)
                     /locus_tag="LNE23_000431"
     CDS             complement(10455..10901)
                     /locus_tag="LNE23_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6930309.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(10994..11239)
                     /locus_tag="LNE23_000432"
     CDS             complement(10994..11239)
                     /locus_tag="LNE23_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBK6930310.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(11354..11932)
                     /locus_tag="LNE23_000433"
     CDS             complement(11354..11932)
                     /locus_tag="LNE23_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6930311.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(12009..12539)
                     /locus_tag="LNE23_000434"
     CDS             complement(12009..12539)
                     /locus_tag="LNE23_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBK6930312.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(12543..13403)
                     /gene="rlmB"
                     /locus_tag="LNE23_000435"
     CDS             complement(12543..13403)
                     /gene="rlmB"
                     /locus_tag="LNE23_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBK6930313.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(13396..13791)
                     /locus_tag="LNE23_000436"
     CDS             complement(13396..13791)
                     /locus_tag="LNE23_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBK6930314.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(13788..15200)
                     /locus_tag="LNE23_000437"
     CDS             complement(13788..15200)
                     /locus_tag="LNE23_000437"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBK6930315.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(15197..15733)
                     /gene="cysE"
                     /locus_tag="LNE23_000438"
     CDS             complement(15197..15733)
                     /gene="cysE"
                     /locus_tag="LNE23_000438"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBK6930316.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(15819..16058)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(16055..17515)
                     /locus_tag="LNE23_000439"
     CDS             complement(16055..17515)
                     /locus_tag="LNE23_000439"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBK6930317.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(17685..18842)
                     /locus_tag="LNE23_000440"
     CDS             complement(17685..18842)
                     /locus_tag="LNE23_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBK6930318.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(18876..20246)
                     /gene="radA"
                     /locus_tag="LNE23_000441"
     CDS             complement(18876..20246)
                     /gene="radA"
                     /locus_tag="LNE23_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBK6930319.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(20309..20836)
                     /locus_tag="LNE23_000442"
     CDS             complement(20309..20836)
                     /locus_tag="LNE23_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBK6930320.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            21066..21872
                     /gene="proB"
                     /locus_tag="LNE23_000443"
     CDS             21066..21872
                     /gene="proB"
                     /locus_tag="LNE23_000443"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBK6930321.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            21869..23116
                     /locus_tag="LNE23_000444"
     CDS             21869..23116
                     /locus_tag="LNE23_000444"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBK6930322.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            23192..23536
                     /locus_tag="LNE23_000445"
     CDS             23192..23536
                     /locus_tag="LNE23_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930323.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(23653..23979)
                     /locus_tag="LNE23_000446"
     CDS             complement(23653..23979)
                     /locus_tag="LNE23_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930324.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(24352..25553)
                     /locus_tag="LNE23_000447"
                     /pseudo
     CDS             complement(24352..25553)
                     /locus_tag="LNE23_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            25670..26185
                     /locus_tag="LNE23_000448"
     CDS             25670..26185
                     /locus_tag="LNE23_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6930325.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(26384..26830)
                     /locus_tag="LNE23_000449"
     CDS             complement(26384..26830)
                     /locus_tag="LNE23_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK6930326.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(26877..28355)
                     /gene="msr(C)"
                     /locus_tag="LNE23_000450"
     CDS             complement(26877..28355)
                     /gene="msr(C)"
                     /locus_tag="LNE23_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK6930327.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(28709..29338)
                     /locus_tag="LNE23_000451"
     CDS             complement(28709..29338)
                     /locus_tag="LNE23_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK6930328.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(29322..29786)
                     /locus_tag="LNE23_000452"
     CDS             complement(29322..29786)
                     /locus_tag="LNE23_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6930329.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(30273..31541)
                     /locus_tag="LNE23_000453"
     CDS             complement(30273..31541)
                     /locus_tag="LNE23_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK6930330.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(31772..32509)
                     /locus_tag="LNE23_000454"
     CDS             complement(31772..32509)
                     /locus_tag="LNE23_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6930331.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(32576..33145)
                     /locus_tag="LNE23_000455"
     CDS             complement(32576..33145)
                     /locus_tag="LNE23_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBK6930332.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            33273..34145
                     /locus_tag="LNE23_000456"
     CDS             33273..34145
                     /locus_tag="LNE23_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6930333.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            34487..35359
                     /locus_tag="LNE23_000457"
     CDS             34487..35359
                     /locus_tag="LNE23_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6930334.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            35356..37314
                     /locus_tag="LNE23_000458"
     CDS             35356..37314
                     /locus_tag="LNE23_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6930335.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(37565..38416)
                     /locus_tag="LNE23_000459"
     CDS             complement(37565..38416)
                     /locus_tag="LNE23_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6930336.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            38539..39006
                     /locus_tag="LNE23_000460"
     CDS             38539..39006
                     /locus_tag="LNE23_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBK6930337.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(39269..39751)
                     /locus_tag="LNE23_000461"
     CDS             complement(39269..39751)
                     /locus_tag="LNE23_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBK6930338.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(39851..40714)
                     /locus_tag="LNE23_000462"
     CDS             complement(39851..40714)
                     /locus_tag="LNE23_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK6930339.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            41312..42160
                     /locus_tag="LNE23_000463"
     CDS             41312..42160
                     /locus_tag="LNE23_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930340.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(42331..42672)
                     /locus_tag="LNE23_000464"
     CDS             complement(42331..42672)
                     /locus_tag="LNE23_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBK6930341.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            42758..43465
                     /locus_tag="LNE23_000465"
     CDS             42758..43465
                     /locus_tag="LNE23_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK6930342.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            43452..43775
                     /locus_tag="LNE23_000466"
     CDS             43452..43775
                     /locus_tag="LNE23_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK6930343.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(43864..44901)
                     /locus_tag="LNE23_000467"
     CDS             complement(43864..44901)
                     /locus_tag="LNE23_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6930344.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(45348..46331)
                     /locus_tag="LNE23_000468"
     CDS             complement(45348..46331)
                     /locus_tag="LNE23_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBK6930345.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(46747..47517)
                     /locus_tag="LNE23_000469"
     CDS             complement(46747..47517)
                     /locus_tag="LNE23_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6930346.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(47510..48445)
                     /locus_tag="LNE23_000470"
     CDS             complement(47510..48445)
                     /locus_tag="LNE23_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6930347.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(49039..50688)
                     /locus_tag="LNE23_000471"
     CDS             complement(49039..50688)
                     /locus_tag="LNE23_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK6930348.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(50883..51779)
                     /locus_tag="LNE23_000472"
     CDS             complement(50883..51779)
                     /locus_tag="LNE23_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6930349.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(51772..52452)
                     /locus_tag="LNE23_000473"
     CDS             complement(51772..52452)
                     /locus_tag="LNE23_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6930350.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(52453..53235)
                     /locus_tag="LNE23_000474"
     CDS             complement(52453..53235)
                     /locus_tag="LNE23_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6930351.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(53228..54145)
                     /locus_tag="LNE23_000475"
     CDS             complement(53228..54145)
                     /locus_tag="LNE23_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6930352.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(54148..54432)
                     /locus_tag="LNE23_000476"
     CDS             complement(54148..54432)
                     /locus_tag="LNE23_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930353.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(55133..55753)
                     /locus_tag="LNE23_000477"
     CDS             complement(55133..55753)
                     /locus_tag="LNE23_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930354.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(55834..>56177)
                     /locus_tag="LNE23_000478"
     CDS             complement(55834..>56177)
                     /locus_tag="LNE23_000478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930355.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
BASE COUNT        16071 a        11960 c         9965 g        18181 t
ORIGIN      
        1 aatgatagca atgttttttc gtgccttaca aaaaaagaag agctgtggct tttgccgcgg
       61 ctcttctttt gtatttttta ttatttagca taatcagttg cacgagtttc ccgaataacg
      121 attactttga tgtgtccagg ataatccagt tcttcttcga ttcgtttacg aatatcacgt
      181 actaaacgaa cagcgtctaa atccgagatt tcttctggct tgaccattac ccgaacttcg
      241 cgtccagctt gaactgcgta acttgattct acaccggcga agctgttcga aatattttcc
      301 agacttctta aacgacggat gtagttttct aatgattcac ttcgcgcacc aggtcttgct
      361 gctgagagcg catctgctgc agctacaagg acagagataa cagaagtagc ttccacatct
      421 ccatgatgag aagcgatcgc gttgattact actgggtttt ctttgtactt ggcagctaat
      481 tccgcaccga tctcaacgtg agatccttca atctcatggt caagtgcttt cccaatatca
      541 tggagcaacc cggcacgttt tgctaattgg atatcttcgc caagctctgc tgctaagata
      601 ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt tttgtccata actagttctg
      661 aatcgcagac gtccgatgat cttgattaga tctggatgaa gtgtatgagc accgacttca
      721 aacgctgcct cttccccgta ttcgcgaata cgttcatcca tttctttacg agatttctcg
      781 accatttctt cgatacgcgc tggatggatt cgtccatctt gtatcagttt ttccaaggtc
      841 atccgtgcga tttctcttcg gattggatcg aatccggaaa gaacaactgc ttccggtgta
      901 tcatcgatga ttaaatcgat accagtcagc gtctcaagcg ttcgaatatt acgaccttct
      961 cgtccaatga tacgaccttt catttcatca ttcggaaggg taacgactga aacagtcgtt
     1021 tctgaaactt gatctgctgc gcagcgttga atcgctaatg ataataagtt tttcgctttg
     1081 cgatctgctt cttctttggc acgctgttca gattctttta ccattagagt taactcatga
     1141 ttcagctctt cttcagtcga tttcatgatg acatctcgtg cgtcctcttt tgataaacca
     1201 gcaatttttt ctagttcttt ttgttgatct tcaatcattt tttcaacgtc tttttctcgc
     1261 tcttcaatta attgctgttt tgaactaagt gcttcttctt tactttcaag tgagttctcg
     1321 cgtttttcta acgagtcatc tttacgattc agcgtttgtt cgcgttgaat caaacgattc
     1381 tcttgagatt ttaattctaa cttactttct ctcagctcac tttcaacttc agaacggtat
     1441 ttctgatttt cttccttagc ttctaacaat gcttcttttt ttagagtttc tgcttctttt
     1501 tttgcactat cgatgattcc agcagctgat gattgtgcac cagctatttc tttctcgtga
     1561 cgtgactttg cgaccataaa ccctaaacca agaccgacaa ttaaaccgat gatagcgagg
     1621 agaatgttga ataccataaa atccacctcc atactatctt tttcattttt atcatctata
     1681 cagcttctat gataaattat tctaaactcg tcaatatgca tcttcgcata tatgtgtaca
     1741 aaaaacatgc acaactttat tctaatgttt gtaccccgta gtgtcaactt taaataaaca
     1801 aagaaacggc tttctactta ttcattaaac gatggttttt aaaataaatg acactagttt
     1861 catataggta aacctttaaa ttgagaaact tttctattaa attattcttt gtagatgaac
     1921 agatattttt attttatgta cccactcgta taattaaata actcacgatt tctttattca
     1981 ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag accagccgtt tttgtttatc
     2041 ataattttta ttcttctact aaaggcaatt cttcttgtcc tttttcatct tctgggacag
     2101 cgacttcttc accaatacca taagctgctc taactagagc agaaacttct gccatcattt
     2161 caggatgatt agccatatag attttagcgt tttcccgtcc ttgaccaatc cgatcttctt
     2221 tataagaata ccaagcaccg cttttatcaa caatgtcttt ttctactgcc atgtctagaa
     2281 gttctccttc ttgtgagatc cctaaaccat acatgacatc tacttcagcg attttgaaag
     2341 gaggtgctac tttgtttttt acgactttga tctttgtacg gttaccaaca atatctgtac
     2401 cttgtttcaa ttgttctgca cgacgtactt ccaaacggat cgttgcgtag aatttcaatg
     2461 cgcgtcctcc aggagtgatt tctggattcc caaacatcac gcctactttt tcacggattt
     2521 ggttgatgaa aatagcgatt gtttttgttt tgttgatcga accagagagt ttacgcaatg
     2581 cttgagacat caaacgtgct tgtaacccga catgtgagtc acccatttcg ccgtcgattt
     2641 ctgctcgtgg aactaaagca gcaactgaat cgactactac gatatctaca gccccacttg
     2701 atactaaagc atcagcgatc tctagacctt gttctcctgt gtcaggctgt gaaagaagta
     2761 gttcatcgat attcacacct aatttttgtg catattgcgg atctaacgca tgctcagcat
     2821 caatgaaagc ggccgttccg ccattttttt gtacttctgc aatagcgtgt agtgcaactg
     2881 ttgttttacc tgaactttca ggtccatata cttcgatgat acgtccacgg gggtaaccgc
     2941 cgacacctaa agcgacatct aacgcaagag agccacttgg aattgtagag atttgttggt
     3001 cgattttttc gcccaatttc ataatagagc ctttaccata gctcttttca atttttttca
     3061 gtgcagcatc taaggctgct ttacgatcat ctgccaatac gataatcctc cttatattta
     3121 caaatcagag caacatttca ttatccttat catatctttt ttagtgaaaa aaagcaagaa
     3181 aaaaccgaac aaatattcgc atttatcatt ttcttaaaag ctgttttctc aaaagatcaa
     3241 atccttgcat aaccgcatta tatctgatag cagcacgatc tctggcaaag tgatattttt
     3301 ccgaaaccac gccgtcttta gatgaaatag cgatccatac tgttcctgca gattgacctt
     3361 ctaacgtgtc cggtccagcc actccggtga aagctacact gtaatctgtt cttgctatct
     3421 cacgtgcttg aatggccatc tgctccacac attctttgct gactgttcca tattctgcta
     3481 atagctccgg atcaatctcc agaaaaccag cttttgtttg gagcgagtag gtaacgaatc
     3541 cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa tgcggcttgg aaggcgccgg
     3601 ctgtcaaact ttccgctgct gttacagtct gcttgttttc tttcagtagt tccactacga
     3661 cttttgctag cgaattatca tcgccgtatc cataaaaata ttcccctact cgttcttgga
     3721 ttttttcttc taatgctaaa agagcttgat tccctgcctg aacatcattt gttttcactg
     3781 tcagtcgtaa tgtcacttca tttggtttgg catatggtgc gatcgttgga ttgatctgtg
     3841 tctcaatcag atcttttagc tcagtgacca gtcgcgattc accaatccca taaaagcgca
     3901 aaactttaga aattagtttt tcttcagaag gaaaacgttc ctctagtaaa ggacgtactt
     3961 gttccacaaa cattggtttc aactcattag gcggaccggg aagaaggata tacgcatgtg
     4021 tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg atttggtaga ggaaccccgc
     4081 cttcgataat ctgggactgt tgcagattgt ttttggtcat ttttcgatgg gttgtttcaa
     4141 aatatgccaa taattttttg tatccttccg tattttgtat gagagactta ccgaggtgag
     4201 ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc taatccgcca cataagagta
     4261 tgagttcact tcttgtttca gctagagcaa tcaactcttc taaacgctgc tgattatctc
     4321 ctacaactgt ttgataataa acttcgatac ctagatcagc tagctgttct gacaagaaag
     4381 tggcattcgt attcacgact tgtcctagta aaagttctgt tccgacagct attatttctg
     4441 ctttcattac ttcccgctcc cttatatagt atacgctgta tttttatatt tttttattta
     4501 aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc tccttgttcc aaataaacag
     4561 tgttctcctg ccaaaacctc ggtcataagt cacaatatac ataaaacatg aaaagctgtt
     4621 ttttgaaatt atcccttgtg actcagagtt agacggcagg ctcacaacct ctccactaaa
     4681 cgatacttac attgatcctt tgaacacacc gctgtttttg atgaagtaat ccacacctga
     4741 atagatagta aagatcagac aagcataaag catgatttga tctactggaa taccgatcag
     4801 gctgaatggg atattattga taaacagcaa gataatagca accatttgtg tagccgtctt
     4861 gacttttccc ggccaagctg cagccatgac ttctccatgt tcgacaagta gtaaacgcaa
     4921 gcctgtgacg gctaattcac gacagacaat gatcgctact acccaaccag gggcctttcc
     4981 ttggtcaacc aacatgataa aagcagtcat aactagcatt ttatctgcca acgggtccgc
     5041 aaacttgcca aaattagtga caaggcctcg cgcacgggca atttttccat ctaaccagtc
     5101 tgtgatacta gctaaagcaa aaataactgc acctactagc tgtgtgacag ccagtgtggt
     5161 gtctcctacg ttaaattgtc cccaatctaa aggtaaacct acaaccaaaa tgaaaatcgg
     5221 gatcatacaa attctaagta cagttaattt atttggtaaa ttcacactat cgactccttt
     5281 cttccctatt ctacgtgact atcagcgaag tttccaccga tattatttac ttttccaaat
     5341 gaatttattc aaaggagtat ttttattgaa attgatcttt ttgaattaac gaagaaggac
     5401 cgcgacacag atttgtcacg atccttcttt tttataccta caagtaaaca gatgcgtaaa
     5461 ctaatttata cgatcattct gcgtattgaa gagtaaggtt cacatttttt ggactctgat
     5521 tgttttcccc tggattaact ggtacggatt ctccattcgc tttgatcgtt gcattgcttg
     5581 ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg taacacggta ctctgtgttt
     5641 catttgctgc cagtgtccct tgataaacat aggcattgtc tactagcaca cctaaccata
     5701 cacgattgac agcagtaaat tcaagggtta caggcttggt agcgtctgtg atatttactg
     5761 cgatggcact gccagaatct tctcctgtag ctattgccat tttcttttct tcacttgatt
     5821 ttgttgttga ttctactgta gaagactctt tggtctgcgt gctttgctct gaggaagagg
     5881 actcctcgac tgatccttct actttaagcg aagaagctgt gtctccgatt attggatcag
     5941 cttgacgatc ttgccaagtc atatagccca cgacaacgac aatcgccaaa gcaaccaatc
     6001 ctaataaaat gacaggaaga cttgtccaaa agcgacttgg atgcgattct tcctgatgca
     6061 ttgcttttct agaagtactt actgtttcag gctgcggacg ttcagggacc gatcctgtca
     6121 atttgctttt cccatcaaaa acatcgacta aatggtctcc atcttctcct acagcttcag
     6181 cgtattgccg aataaatgcc cgcacgtaaa atttaccagg caattggtca aaatcatcat
     6241 tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat ctgctgtaat tcatctaaag
     6301 atattttttt attcaggcgt gcttgccgta atttttctcc gatggtttca aaagccactc
     6361 gttcatcttc tccagtctgt ttgattctcc gaagcatctg ctgttgcaac gaaagacgtt
     6421 atgtttgggt gctgtgacaa aagtcatgtc acagctcctt ttttccgaat aaacattgtt
     6481 ctcctgccaa ctcctcggtc acaagtcaca atatccaata aatataagaa gctgtttttt
     6541 gaacttgttc cttgtgactc ggagttaaac ggcaggctca cgacctcttt cttaaacggt
     6601 gttcaatcag ctgttcctcg gtattagctc aaaaaccggc aaaatataag gagatatttt
     6661 tccgtatgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct tccttaaacg
     6721 gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca ttaagaagct
     6781 gtttttactt ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttggta
     6841 cacggtgttc cagaagtagc ttcttcggca ataagccgaa atatcacaaa aatttgagaa
     6901 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca caacctcttt
     6961 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
     7021 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
     7081 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
     7141 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
     7201 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
     7261 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
     7321 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
     7381 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
     7441 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
     7501 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
     7561 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
     7621 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
     7681 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
     7741 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
     7801 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
     7861 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
     7921 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     7981 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     8041 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     8101 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     8161 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     8221 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     8281 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     8341 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     8401 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     8461 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     8521 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     8581 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     8641 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     8701 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     8761 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     8821 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     8881 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     8941 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     9001 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     9061 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     9121 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     9181 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     9241 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     9301 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     9361 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     9421 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     9481 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     9541 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     9601 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     9661 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     9721 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     9781 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     9841 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     9901 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     9961 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
    10021 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
    10081 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
    10141 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
    10201 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
    10261 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
    10321 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
    10381 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
    10441 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
    10501 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
    10561 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
    10621 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
    10681 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
    10741 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
    10801 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
    10861 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
    10921 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
    10981 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
    11041 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
    11101 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
    11161 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
    11221 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
    11281 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
    11341 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
    11401 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
    11461 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
    11521 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aatcgctaaa ggttcttgca
    11581 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
    11641 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
    11701 tgcttataat gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
    11761 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
    11821 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
    11881 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
    11941 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
    12001 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
    12061 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
    12121 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
    12181 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
    12241 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
    12301 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
    12361 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
    12421 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
    12481 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
    12541 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
    12601 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
    12661 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
    12721 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
    12781 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
    12841 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
    12901 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
    12961 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
    13021 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
    13081 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
    13141 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
    13201 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
    13261 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
    13321 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
    13381 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
    13441 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
    13501 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
    13561 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
    13621 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
    13681 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
    13741 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
    13801 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
    13861 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
    13921 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
    13981 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
    14041 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
    14101 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
    14161 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
    14221 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
    14281 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
    14341 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
    14401 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
    14461 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
    14521 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
    14581 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
    14641 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
    14701 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
    14761 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
    14821 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
    14881 ggctcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
    14941 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
    15001 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
    15061 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
    15121 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
    15181 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
    15241 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
    15301 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
    15361 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
    15421 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
    15481 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
    15541 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
    15601 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
    15661 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
    15721 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
    15781 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
    15841 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
    15901 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
    15961 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
    16021 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
    16081 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
    16141 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
    16201 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
    16261 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
    16321 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
    16381 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
    16441 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
    16501 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
    16561 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
    16621 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
    16681 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
    16741 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
    16801 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
    16861 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
    16921 caaaattata agttggcata ccatcgcgtt tttgtatgac aaagtcgcca ccgatattat
    16981 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    17041 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    17101 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    17161 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    17221 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    17281 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    17341 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    17401 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    17461 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    17521 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    17581 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    17641 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    17701 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    17761 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    17821 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    17881 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    17941 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    18001 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    18061 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    18121 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    18181 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    18241 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    18301 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    18361 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    18421 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    18481 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    18541 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    18601 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    18661 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    18721 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    18781 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    18841 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    18901 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    18961 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    19021 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    19081 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    19141 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    19201 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    19261 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    19321 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    19381 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    19441 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    19501 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    19561 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    19621 atcccatttg ttttagcgat tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    19681 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    19741 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    19801 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    19861 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    19921 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    19981 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    20041 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    20101 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    20161 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    20221 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    20281 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    20341 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    20401 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    20461 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    20521 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    20581 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    20641 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    20701 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    20761 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    20821 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    20881 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    20941 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    21001 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    21061 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    21121 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    21181 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    21241 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    21301 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    21361 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    21421 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    21481 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    21541 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    21601 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    21661 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    21721 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    21781 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    21841 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    21901 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    21961 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    22021 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    22081 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    22141 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    22201 cgtgccacta ggcgtgaccg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    22261 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    22321 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    22381 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    22441 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    22501 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    22561 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    22621 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    22681 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    22741 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    22801 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    22861 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    22921 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    22981 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    23041 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    23101 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    23161 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    23221 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    23281 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    23341 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    23401 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    23461 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    23521 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    23581 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    23641 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    23701 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    23761 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    23821 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    23881 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    23941 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    24001 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    24061 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    24121 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    24181 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaaa
    24241 agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagaagct atttttgttt
    24301 ggctgttctt atgacttgat gcaggacagc tttttcacaa cctctgattt cttattcttc
    24361 ttcatttgta gcaaaataat tacggtgata aatgatacaa catacactga taagaacaat
    24421 ggcacctgca aaataaaacg catagctttc accaatatgt tcaccaatca cacctgcaat
    24481 aaatggtgct aaagctccac cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt
    24541 tcgttgcaga cctggtattt ccatagcaat cgtcgtcaac agtgtattga taattccttg
    24601 gaaaaatcct gctaacacaa tgctgatcgg tacaaggact ggcatagaag ttccagctcc
    24661 taataacagc aaacaaataa tgaataagac aaatgctgct aaaattgtcc gataagtcgt
    24721 aaacttccgc tcaagagccg gtgcaacgaa aatcgaagca atagccagaa gcacacccca
    24781 gccaaaaaat acaaaaccga cttctaattc agaatagcca atcaataaaa atggcgcata
    24841 cgcaagtaaa gtaaagaatc cgaaattata taacaatgcg attagtccaa ctgaacgaag
    24901 tttatgattg cttaacgctt tgacaccttc ccaaaggcct acttttctct ttggtttctc
    24961 agtttcaggt aaaagtaccg ctagggctat aaaaccgcaa aacatcaaaa atgctacccc
    25021 aaaaaatgga taacgccatg aaccactgcc caagacccca ccgactagag ggccaacagc
    25081 cattccgcag ccaagtgctg cttcatacat catgattgct ttttctgtgt taccaactaa
    25141 aatactgacg atcgttgcta aagcagtaga gacaaataaa gcattcccga caccccagcc
    25201 ggcacgtaac ccaatcagca ttccgatatt cccagataat ccgccaagac cagcgaatac
    25261 aacgatgata aacaatcctg tcatcaatgt tttttcaaac cgatccgact agataagaaa
    25321 cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa cagggtcgtt
    25381 tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc tactagaccc
    25441 actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact tttccccttt
    25501 gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc catccctttc
    25561 tctctattag catcatacat gatttatttt acatgtaaat atgcacatgt aaaacgtgga
    25621 aactttactg tgatataata aagaaaataa gacgagaggt aggattttta tgaatacttt
    25681 agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg aattgaagca
    25741 gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc ttgccaagtt
    25801 agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa aaaagatcta
    25861 ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg aaactcccgc
    25921 tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc tcgataaaac
    25981 aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca tgcaaaaaaa
    26041 tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac gttatgttgt
    26101 gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc aagtaagaga
    26161 acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa tatctattga
    26221 agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc gatccgaacg
    26281 acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca agagactatg
    26341 acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca ttactttgaa
    26401 ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac ggtaaagcgg
    26461 caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca ggtattcctt
    26521 gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc atcaagcatg
    26581 aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta aaggcaattt
    26641 ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg aatccaggca
    26701 ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa ttatacgcgc
    26761 tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct tcttttttat
    26821 ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta gcacatttaa
    26881 aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac ttcatcagcc
    26941 gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg atatatttgc
    27001 attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg ttcatctaac
    27061 agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt cgcttctccc
    27121 ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa gtgattcagc
    27181 aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg aatgatggat
    27241 tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac tttcggagag
    27301 agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt acctgaacca
    27361 ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc atttaccagt
    27421 aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata gcggctgtgg
    27481 agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg cttggttgtt
    27541 tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat ggctttttgt
    27601 acactgtctt tttgtttaga ggaagacaac cgatccggtc tgatttgttg ctttttcttt
    27661 ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat cgatttttcc
    27721 aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt tttctccaat
    27781 tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc tccgtctttt
    27841 acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact aacaaaaacc
    27901 agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt tctatctaga
    27961 tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag acgtatcaac
    28021 tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc cagtcttaac
    28081 tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata ggttaattga
    28141 ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa aagttcaccg
    28201 gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat ccctatacga
    28261 tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc aaagctgact
    28321 tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct atttcatgta
    28381 aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa aaactgcatc
    28441 acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct gtcatagctt
    28501 tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa ttgttgttta
    28561 tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa tttcatcgat
    28621 gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca aaaaaagcaa
    28681 tagctactga atagtaactg aaaacctttt attcgaacgt agtgatcttt tctggtgcta
    28741 aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata ataactggaa
    28801 cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg aatccaccaa
    28861 ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata gccgctaagc
    28921 tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg taataaggaa
    28981 tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat tcgtaagctt
    29041 ggttatagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc atgaccattg
    29101 cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag atttcttttg
    29161 cttcctcgct ttcagcgacc acaaagcgcc atggttgcat attgacagaa gaaggtgcag
    29221 tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg gattttgtat gtttcatcat
    29281 atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt tgttccatta
    29341 ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa tttatctaaa
    29401 agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac ggaacctgta
    29461 tctttatggt ccatttcaca atgctcgatt ttcttctggg ctttttctgt tagcgctaca
    29521 tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt tgcttccaat
    29581 accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg caaactgctt
    29641 tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt aagacttata
    29701 ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat ttgatataaa
    29761 aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga ctcgtcaacg
    29821 attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcagc tgtgtaataa
    29881 aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa tgaacggaaa
    29941 aaatgataaa gaaagcacaa cgctaaaacc aagattattg attcttatca aactatagaa
    30001 gatagctctt ctgcagtaga aaattttaca ccgccaaaaa tttccgaaat cgccttttgc
    30061 aagaatcccg tcgaatatta gaaattggat cagtatgctt aaacagtaat aaaaaagtta
    30121 tgacaagatg ttgtcacaac cttttttgag taaacggtgt tcaaaaactt gcatctgcgg
    30181 taataagttc aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg ttcttactac
    30241 ttgatgcagg gcagcttttt cactacctct ttttaataac cataaaatcc tattccttca
    30301 tatctccagc ccatcggtgt tagccaatct ctttccttag cactcaaggt atagtgatgg
    30361 gtaccagttt tttcatttgg attaaatagt cgataaattg gcttggaacc accagaataa
    30421 aaacttagac cttcataacg ccaaccgatt tttgttaaag agttgatctc agattgtgcc
    30481 attgtgtaat gatggtctcc actgtaagga ttgtataaac ggtagacagg ttgtccgctg
    30541 tttggtgctt gccaagcaat cccttcatat tcccagccta cagttggcag ataatttttt
    30601 tctccggcat tcaatgtata taaatgttct cctgtatgac ggttatacag acgatacatc
    30661 ggagaagttg aagttggtgc tgtatcatac tgtgtcagat tataggtttc gattacattg
    30721 ttcaatgtag aaccataatt aggcgcagtg gcataacgac ctgttagcca agccgtagca
    30781 tctttgtaag agttcgtatt gcttttccat gcaccggaat agtagtaaac gcctggctga
    30841 aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg attctttgta cgaaggatat
    30901 tttctaaaag cggcattcac cgtaatccat tgcccattca catattccga agtaggcatc
    30961 gtaacagatt ggccattata gcttccttta atcccaaata gattgtaatt aggggcagaa
    31021 gccaaagtac tgttccccca gccactttca acgatggctt gagcaatcat gactgatgca
    31081 tataagtcat tggcacttgc caaactttgt gcagttgagg caatactatc aatgaacgct
    31141 tgttgagaat tagcagatga gcgagcataa aaagcatagc cagtatagtt ttcgaattca
    31201 gacaaatctg cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg gttcgtgatt
    31261 tcttctgcat tctctggatt gttctgagct tctgctgatg tactgtcttc ggtttgtgtt
    31321 tgttctgttg tttcagttgt tggttcttcg gttgaaattg tattttctga gacagtagct
    31381 gactgcaaag tactctctgt atcgttttca attcctgttt ctgaagaact cgatgtctga
    31441 gttgcgacag tttctgtcga ttcttcagct agcactgttg tgttggcaac tgctagattt
    31501 cctaaaagaa taacgctaaa taataaattc tttgctttca tcttttttct cctttgaatt
    31561 atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca atgtccatat
    31621 ttaaataaca tttgtttctt tttgataaat ttgctttttc tacataaaaa gacgtaaatg
    31681 aagaatattc ttctccattc acgccttaaa ttgatggtcg tttgaataac tatttaacaa
    31741 gtaattctct ttggatatta tccgttatgg attatacttc ctgtgtcgtc ggacggataa
    31801 tcatgttact gacagacatc cggtctggcg tttctatcgc aaatgctaca gcagaagcta
    31861 tatcttcgga ggtcaatccc cattcttttt gcgcttcatg aagttcaaga gcagcctttt
    31921 tgtcgctgat ggtttgatat aattcagttt gaacagctcc tggagagatg atcgtagatt
    31981 tgatgttatt ttgccgttgc tcttgacgta atccttccat gattgcacgg acagcaaatt
    32041 ttgttccgca ataaacggcg gaatcaggat aaacaacatg tcccgccaca gaatcagttg
    32101 aaatgatatg cccagatttt tgtttttcca tgattggaag tacggcggaa attccattta
    32161 acacacccat gatgttgata tcaagcatat tttgccattc atcacggtga ccttcaacta
    32221 atggagcggt tggcatgatt cctgcattat tgtacaatac atcgattcga ccgaactttt
    32281 ccatcgctaa atcgataact gcttggactt gtgcaaaatc tcttacatct gctgtttgaa
    32341 tatatagctc agcttctggt aaagattctt ttattgcttt taggcgatct tctcggcgtg
    32401 cagctataac tagtttagcc cctttttctg ctaatagctt cgttgttgct tcaccgattc
    32461 cgcttgaagc gcccatgatg acaatcactt tttctgataa atcattcatt ctattctcct
    32521 cctgttttga aaaatctctt gcttatactc actatctctt atttcatctt actgttcaaa
    32581 taatccgttc gacaggtttg acgattttgg cagggattcc tgcaacgatg gtattgtctg
    32641 ggacatcttt cgtaacagtt gcattcgctg caacaatggc attctcgcca atcgttacgc
    32701 ctggcagaat agttgctcct gctccgatcc aagcattttt cttgatgtgg attggcttta
    32761 ctgacaagcc tcttcgttct tttggatcgg ttagatggtt caccgtgatg aggttgacac
    32821 gaggaccaat caatactgaa tcttctaaac agatgccacc taagtcaacg aacatcactt
    32881 gacgattgat aaagacattt ttccctatac taatatgttt gccgaaatct gtataaaaag
    32941 gcagcagaac agtcaccgta tcatctactt tgtcactaat gatattttct agccgtttgc
    33001 ggatatctgc tggttcatgg tacccagtat tcagcgctgc taaaggctct tcgttttcag
    33061 cttggatctg atggatcact tcaaagagcg gatccgttac tcgaatctct ttattgacga
    33121 tttgtttttc gatgaggctt gtcattttgc tccctccttt tatccttact ttataacgga
    33181 cggcttatca tgtctaatac ttataaaaca tacttaacta ttccttttaa ctatacttag
    33241 tggtatgctt ttttaaaaac aggagggaaa aaatggatat tcgtatactg acgtattttt
    33301 tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa atctctgcat atcacgcaac
    33361 ctactctctc aaaacaactg aaagatttag aagaagaact tggtgtgaca cttttcactc
    33421 ggggctcccg agagatacag ttgacggatt ctggtcgata tctttattca aaaggaaaag
    33481 aaatcttgtc gttggtagaa aaaacggcga acaatttaac aaatgacgca atcatctctg
    33541 gagaaatcta cattggcgga ggcgaaacta aagcaatctc tttcatttct gatagccttc
    33601 attcgttgat caaggcgcat cctgatatcc agtttcattt atatagcggg aatgcagatg
    33661 atatcataga caaactggat agcggattac ttgattttgg actcgtaatc gaaccgacaa
    33721 acaaacaaaa ataccagtca tttcgactgc cagcccaaga tcgttgggga cttttgatac
    33781 atcgaaatca tcctttagca aagcaaaccg tcatccatcc gcaggaactg aaaactgttc
    33841 ctttgatcat ttcccggcaa acgtcagtgg acagtcaact agctgaatgg ctaggtttca
    33901 gcatagatca gttatcgatc gtcggaacat ataacttatt gtacaatgct tctttgatgg
    33961 tcgataacgg gacttttggt gcattagctc tagatgggat catcaatact tatgggacgg
    34021 aaaaaatatt catcccactc gatccgccat tacaagcttc actgaatctc atttggaaaa
    34081 aagaccagcc tctttctcct gctgctcgta aatttttaga tgtcctaacg gagaaataca
    34141 gttaagtaga gtttttttcc ttcgctttca gctttttaac aacgaataag ataagcaaaa
    34201 gatccaacga ttaaataaca taagtttctt aatttatttc ttccttttat ttctgataat
    34261 tccagtaaat tactataatt ataaaaaaag aattgacaaa acataactgt ctatataaaa
    34321 ttgattacgt taacaataat tcgtttcatg tgactagatc atactaggca tgaaaaatct
    34381 ctgaggaaca tggtttccct aggaggtttt tcgtgtctat ttttgtttgg ccgcaatcaa
    34441 ctggtctagc aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga aaatcaaaaa
    34501 gatctttaat caaaatgcag tattggtaga tgacggtgga caggaaaaag tagctattgg
    34561 aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg atgtcgaacg
    34621 gatatttgtc atggaaccag aaggtcaaat caagttgcaa aatttactta atcagattga
    34681 tgaacgcttt ttatttgcag cagaaacgat cattgatcat gcggaaactg ttttgatgga
    34741 aaagttgaat gaacatgtat tgatcgcttt gaccgaccac attgcttttt ctgcggagaa
    34801 cattaataac ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag tgctttacag
    34861 tgatgaattc agtattgctc aatgggcagt agagtatctg acaaaagagc ttgggattcc
    34921 atatacttat gacgaagcag gatatatcgc aatccatatt cacagcggac gaagcggtcg
    34981 gaacaataac catcgcagta tccgtgaagt gacgattgta tctgacatca tccatttagt
    35041 ggaatcagaa ttggaaacag atatccattc agaaccattt tcactgaatt attcaagact
    35101 ggtgaatcat ttacgactgc ttctacaacg aacccatgcg caacaatatg ctgttttaga
    35161 cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa agctataaga tttctaaaaa
    35221 aatccgtgtg ttgctgacca aagaatacca gctcgcgatc acgaaagaag aactgggtta
    35281 cttggccatc catatcgagc gtttaagaag cgcaatcgta caaacaaatg ttaacaatca
    35341 tgaggaggaa aaaaattgaa agcatatcta caacgaatgg gacgttccct tcaacttcca
    35401 gttgcggtct tacctgctgc tgctttacta gtagggatcg gaaactggtg ggcatcttat
    35461 agtaacgaca ttgtcgctca ttttttacaa gctggcggta acgcagtttt aggtcaatta
    35521 cctttgctgt ttgccgttgg gttagcacta gggatgtcag tggacaaatc aggtgctgct
    35581 gccttggcag gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac tgattcagta
    35641 gctactttat tgaatatcaa accagaatca gttgatccat cttttgctca gatcagtaac
    35701 gtatttattg gtattattgc cggattgatt gcggcagctc tttataatcg tttccacgaa
    35761 gcaaaattac cgatggcttt atctttcttt agcggcaagc gtctagtgcc aatcttggct
    35821 gcctttgtca tgttgctgat cagtgcagtg ctactgctcg tatggcctcc ggtttactct
    35881 gctttagttt cttttgctaa attcattttg agtttaggct ggggtggtgc aggtctttat
    35941 ggcttcttca accgtttact gatccctaca ggattgcatc aagcaatgaa ctccgtgttc
    36001 tggtttgacg tagcaggaat caatgatatt ggtaaatttt tggcttctga aggaacaaaa
    36061 ggcgtaacag gaatgtatca agctggattt ttcccaatca tgatgttcgg gcttccagca
    36121 ggagcatatg ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac agcttccttg
    36181 atgctagcag cagcttttgc ttcattcttt actggtgtga cagaaccact tgaattttca
    36241 tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg gatttcttta
    36301 gcagtgagtg cattcttcca ttggacagca ggatttgcct ttagtgccgg atttgtcgat
    36361 tacttcctat ctttgaaaaa tccgattgcg aatcagccat tgatgttgat cgtccaagga
    36421 ttagtaacag cagtgattta ctactttggc tttaactttg ctatcaaaaa attccatctg
    36481 atgactcctg gccgcgagga agcagatctt tcagatgacg atactgcaac gactaattca
    36541 gacaataaat atgcggcaca agcaagcaaa atctacgctg cattaggcgg agccgacaat
    36601 gtaacttcga tcgataactg tactacacgc cttcgtttgc aagtcaaaga tacaggcctg
    36661 atcgatcaaa ataaaatcaa agccactggc gtacctggta tgaagatcat cgacggaaaa
    36721 aatgcgcaag tcatcgtagg tacagaagta caattcgttg cagatgagat ggcaaaacta
    36781 catggtggtg ctgcagcacg acctgcacaa acaaacaccg tggttaaaac agaaacattt
    36841 gccggtgaaa cgatcgaaca agatatttac gctatcgcaa atgggaaact gattccaatc
    36901 actgaagttt cagatgatgt attcgcggaa aaaatgatgg gtgacggcta tgctgtatta
    36961 ccagaaaatg gagaaatctt ctctccaatc gctggtacga tcacaaacat tttcccaacg
    37021 aaacatgctg tcggaatcca aacagatgct ggtatcgaag tcttgcttca tatgggcatc
    37081 aacactgttg acttaaaagg cgaaccattc actttatatg tagaagaagg tcaaaaagtc
    37141 gcaagaggtc aattgattgc tttagttgat cttgccgcta ttcaatctgc aggcaaaaac
    37201 acggatatgg tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga aatcgaaccc
    37261 gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc ctgagccatc
    37321 gtcttcccca gactttttta tcgataaaca aacaggagct gagacaaaaa tcttgtctca
    37381 gctccttttt ccgaataaaa acggcgttct cctgccaact ccgcggtaat aagtccagct
    37441 aaacgaaaca tatgaagagc tattttttat ttgactcttc ttattacttg atgcagaaca
    37501 gctttttcac aaaatttttc ttcatactca aaactaatcg gctttttcac aacctcttta
    37561 tacattattc ctgatccaat atttctttta atgctgcttc aagagaagtt aatggttttc
    37621 ccaatacttt ttctaaatca tcagaggaaa tagctaactg atccttttta atatcatgtt
    37681 ggatcgctaa aaacatgtca gctgcttctt gagggaatcc ggcttctttt agattttcga
    37741 caaacgctgc atcacttgct tcaataattt ctaactcttt acctgtcgca cgctctaatg
    37801 ccttggctaa ttcttcatag tcagttagat tgccggataa ttctaagatt tctggagagt
    37861 cactttctac tagtactttt gctgcagctt ctgcatattc acgtttcaat gcccaacctg
    37921 tttttccatt tcctgcagca taaacgaatt gaccagtttt taaagcctgc ccaatgatag
    37981 gtaattcatt ctctacatac caattattcc gtaaaaatgt atgggaaata cctgaatcct
    38041 taatcatttt ttcagtgaat tggtgatctg gcgctaatac actgatcgag tgatcggcat
    38101 ctgcaaaact tgtataagcg atataagata ccccagctgc ttgtgctgcc ttcactacat
    38161 tcttatgttc ggcttgacga tttcctggaa cgccagagac aaaaagcaat cgatctattc
    38221 ctttcaacgc ttctttcatt gagtctaaat catcataatc accaatacgt atattaaatc
    38281 cagcttcttt cagtgcttct cctttttctt cactacgtac tagtacatag atttcatttt
    38341 gtggaaccat cttttttaag aattccagcg cataaccacc aaaacctcca gttgctcctg
    38401 ttactaaata atgcatattt gccactccct attcttttta tttgttaaga tttacagctg
    38461 tacatttata aattacacct ttatcataaa gagtgtggta agataagtca agcaaaaaaa
    38521 atgaaaggat gacaactgat gaagtattct ctgcaattta gtgatgcaat ccatgtactt
    38581 gcctatattg aaatgtttca agatacccga tcactttcta gtgagatgat tgccagtagt
    38641 atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg
    38701 atctttacaa aaaccggaaa accgcagcct tcccttgcca aaagtccaga tcagatttct
    38761 ttactagatg tctatcgcag catcgaaggt aatacacagc ttatccaagt cgatccaaaa
    38821 acaaatccaa attgcgtcgt tggtgcgaat atccaagaag ctttagaaga aagctacgct
    38881 cggctgcaga aaaaggcaga agaagaaatg gctaagatca cacttgatag tctgatacat
    38941 aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca caggcctcgt agagagattt
    39001 ttataattct ctctactgtg cctgcatttt tctgatcgga atcgtctaca taaattcttt
    39061 tattctccgg gggacaaaag ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa
    39121 agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt agctgaccct cggcattagt
    39181 tctgaaaaat gcaaaatatg gaaagctatt tcccattttt cctctctaat gctcaggtca
    39241 aagcgctact ttcacgacct ctatattatc atggtttatt gactaccggt atccagattt
    39301 ctcccatata tttcccgttg gtaactccca tttcgactac agcatttgga cccccaacat
    39361 aggcgtaatc ttctacttct cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc
    39421 ttaattcttc aggcgattct gctaaagcgc ttacaacaag atacgttcct tttgggaatg
    39481 ggatgattcg cgtagtgttt ggaacatcct gactcgtttg aacaccgaca tagtacatca
    39541 ttttcccgtt atagggttcg ttgactgtaa agaaataatg attttcagca gcttgtttca
    39601 atgcatctat tgttccatta ccgataactt tttgctcaaa tgcttgtttc tctgcagcca
    39661 aaccgacaaa atccgtattg ctactcttca attccacacc aatacccgtc accgtaaaat
    39721 ctggtttttc ttcgatccta tactttgcca tttctcccac cctttctatt atattaagtt
    39781 catcttacat aaaattaaag ggaaatgaca cgattacgct cttttttcta gggatatttt
    39841 tattttttaa ctattttttt gctttcactg caatccaaat ctcactgtaa acattactga
    39901 aatcctctcc ccattttgta aaagagatct cgggagcttc gactaaccca taatctgaag
    39961 aaggaagcca ttctgcatag attttccccc aagtatcttg taaagtttgg ggaaatggcc
    40021 ctttattagg aaaaatggcc caagtgtgtt cttctatagc aagcatttct aaatcagcgt
    40081 acggattttc ttttgtcgta gcaaagccaa tcatgtgcat caaactgccc ttttcttcca
    40141 agcggtctcc atcaaatcca taagaaacgt tgattacttg atttggatat aaatctccta
    40201 acttatgcat ctcttccctt tgagtctccg tgatcgtctg cgctaacttt tgtatttctt
    40261 gattgactcc ttcaaatttg acaggaactt ttttcgccac acctgccaca taaaactgtt
    40321 ctttcttttc gatacgaaat tccatcgatt ttcctcctct gacatcaata aaaaatgtca
    40381 agcgtggaaa agatttttgt atgccgtatc tactgatttc tgaaggcaga tatcctgacc
    40441 aatcacgaaa agctcttgaa aaaccttcga ctgtttcata tccatatcta aacgccgttt
    40501 ctgtcactct tgatccatct agaagatcgc gattagccaa agccaatcta cggtttttga
    40561 tatagtccat tagcgacatc cctgcaataa acgtaaagat gcgttttaat tgatattcag
    40621 agattcctgt attttcggct attattttat aggaaagtcc ttctgtcaaa tgttcttcga
    40681 tgtaatcgat caattggttc agatctttaa gcatatttat cttcctcctt gattgccttt
    40741 ttatcatatt atcaaagaaa gtcaattact tgatgaatca ggaaccaatt tagtcagatg
    40801 tagaaaatgt tgtatggatc taaagaaaat tctcaagcag cgtttcgtaa cggataattg
    40861 gtattatctg cctatctatg ttttaccttt agaaaaataa attttgggga agacagattc
    40921 taagtaaaca aagcggtaaa aatatctttt attcccttag ttaatccgct tactacctac
    40981 tgtacaaagc aaattgttgt tcaaactgct gatcagaacg ctgcttggat ttttctttgc
    41041 gatacatcat acctctttta tcaattggaa caattattta tttctaacaa aatcacttca
    41101 tttttgtcag ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta
    41161 ctttttctca cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa
    41221 agtgattatc aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat
    41281 ataaataagt gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga
    41341 aaacgttttg gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta
    41401 tacaagcaag tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac
    41461 ttgggatgaa aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg
    41521 caaacacatg gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta
    41581 ctcactttcc gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct
    41641 tttaacagag attcttatag aacatccgca ctttatcgaa cgcttcaaag ttaagatgcc
    41701 atttcatgca ataaattatt cagataaatt aacaactatc atccataaat acgatcatat
    41761 tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat
    41821 ccaagaagca gttgaatcgc ttattcagca aaatgcctat cttcccgcat tcgaattgat
    41881 caatgaagtg attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct
    41941 tcttctgata gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc
    42001 agaaaaaagg caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta
    42061 ttcaaaacgc tactctataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa
    42121 ccaaaagaaa aaaatagata aaaaactaaa gaaacggtaa agaaagacga tttcaatgaa
    42181 aaatagcaag taaacgataa caaccatcct aaatccacaa gaaatacagc agaccttgct
    42241 ttaaaaacac gaatcatgca aaggatgaag taaagaagac ttaacgcgta cccaatatta
    42301 gtcatttgac tattaggtac gcgttttgtt ttataagtca tgacaaattt ccttcgttgc
    42361 tggatagatt ttccggcatt gttgatagat tttatgatac ttttctacat tttgtaagag
    42421 aggttccgct gcatctgtat attggataaa attctgacaa caactttcag gtgtgtcgaa
    42481 ccaccctaac cctagtgcag caatcattgt tgcgcctaat gcaggtcctt cctccatact
    42541 taaacaatag accgttgcat cgaaaatatc agcttggatt tgtagccatg caagattttt
    42601 cgcgcctcct cccaccgaaa caatacgtgt aaatctgcga cccgctagtt ctttacgttt
    42661 caatcccacc attgacaaat gctcattaaa aatttagaat attcacaagt ttaacgacaa
    42721 ttatgaaaat ttatgtcgtg ctaaaggagt tttgttattg gatgaaaagt tagatataaa
    42781 aactgcgatc aaagacacgt tacctaccgt tttcggttat atcggtattg gacttgcatt
    42841 tggtatcgtt gggaaagctg ccggatttca tccactagtc gtcacgttga tgtccctgct
    42901 ggtctatgct ggttctgccc aatttatcac agtcagcatg cttgctagtc acagcccatt
    42961 gctttccatc gttttctcga cctttctagt caattcccga atgattctga tgagtatgac
    43021 gattgctcct tatttcaaga aaaatcggct gcttcaaaat ctgctgattg gcacattgct
    43081 gacagatgaa agtttcgcac tgggaatgaa taaattgaac tatactggac aaaagttaaa
    43141 ctttcgttgg atgaatacag ctaattggat ttcttatttg acgtgggtgg gttcttctct
    43201 tgttggcgca cttttaggga attttattac tgatccgaaa aagtttggtt tggatttcgc
    43261 catcgtcgct atgtttatcg ggttacttta tcttcaagtc atttctgata gaaacacctc
    43321 taaacgatta cagttgatcc tgattggtct cactttgatt ctggtatatg tcggactgat
    43381 ttttatccca agcaacttag tgattgtagt tgtaacactg attgggtgcg gattgggggt
    43441 gtggatcaaa catgccttct tttgagttta cattgctgac tattttaggt tgtactttag
    43501 caacttggct ttctagggtc ctaccatttg tcttactgaa aaaattcgat ttaccgcaac
    43561 cgttgcttga gtatttaagt tttgtcccga tcgtcattat gtctgcttta tggttcagca
    43621 gtctatttac ccaaaatatc ggacatctgc cgcagatcaa tctcgaaaat gctttggctt
    43681 cagttcctac acttttagct gctattcttt caaaaagctt attggtgatc gtcttggcag
    43741 gaattctttc cctttcgttg attcgcttat tataaataaa aaaacaagag cttatggcaa
    43801 attactgtca tgcgctcttg tttttatcaa agtcaaataa gaaaaaaatc ggcgtattct
    43861 tgcttatccg ttgaacggac cactaacaac gacttttcct aacatatgtc ctgtttctac
    43921 gtctttggtt gcttgtttca agttcttcgc attcatccca tctgaataga ccttgctcaa
    43981 tgtactgcgg ataatccctt catttgctaa agcagcaatg tgcgctaaag cttctccttg
    44041 cgtttcgatt tcatagttat agtctgtttt agcgaacata tactcccagt cgaagctgat
    44101 agattggttt ttccaaacac ctatatctaa cggctctttg atccctacga tcgttcccac
    44161 atgtccaaat ggtcggacaa ggtgcttgat ttgatcaaag tagcgggtaa tatcgaataa
    44221 tacagcgaca tactctactt gatcgattcc taattcacga aaacttttac ttaagtcgtg
    44281 atgataatcg attggatagt ctacacctgt ttttttcagc cattcaaaat tcttcggact
    44341 tgcagtcgcg tagacttcta ggccagccca atgcgctagt tgattcaaaa tcgaaccgac
    44401 gcccccggat ccgttgatca ctaagatttt tttcccgcga ttggcatttt cttctggagt
    44461 tagaccaaat ttttcaaata acaattcata agcggtcaat gaggtcaacg gcaaggctgc
    44521 agcttcttca tctgaaagat tttttggtgc aagagcgaca atacgttcgt caactagttg
    44581 gtattcttga ttgcttcctg ctcttgttgt tgtacctgcg taaaaaacgc gatcgccgac
    44641 agaaaatttt tgcacttcgt cacctacagc aacaactttc ccgactccat caaaacctaa
    44701 gatacgtaaa gcatttctta tcccgttatc ttggcgaagt ttcgtatcga caggattaac
    44761 agatactgcg ttgactttta ccagaagatc ccgtgaactg ggtacaggta tctgttcttt
    44821 ttcatcaata aagctttttg gatcatcgat cggcaaccct tcataaaaac cgactacctt
    44881 cattaattcc ttttctgaca tacgaattcc tccttagctt catcaagttg gagcatccat
    44941 acagtgttca tttcaatata tatagtatgc tatttgttcc acctttttta ttcatcataa
    45001 ttattctctt acttaaagta aactatttgc acttttttgg ttaaaaaaga ggttgcgaca
    45061 agacttttgt cacaacctct tataaacggt gttctcctac caactcttcg gtcacaagtc
    45121 aaaatattca tcaaacatga gaagctgttt tttgaaattg ctccttgtga ctcagagtta
    45181 aacggcaggc tcacaacctc tgatataaac ggtgttcaat caactgaccc tcggtattag
    45241 ctcaaaaaac gacaaaatat gaagagctat ttttgtttgg ctgttcttat tacttgatac
    45301 aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca ttgttcactg
    45361 aaaagcgttg gatgttcttt ttcaaaggtc tctaagatct ctcgtacttc atcagtagat
    45421 ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt gatatagatt
    45481 ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata atgatcttgc
    45541 agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc cctcgggtct
    45601 ttttcaaatg cataaggatt cttgaaaatc cctcggccaa tcatgatgcc atcaacgcca
    45661 tatttctcgg ccaattcttc tcctgtctgg cgatcgggga tatctccatt gatcgtgatt
    45721 agtgtttgtg gagcgatttg gtcacgtaaa gcaatgattt taggaatgac ttcccaatgt
    45781 gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt ggctatatct
    45841 tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata gccgatacgc
    45901 gtcttgacgc tgacaggcaa accgccagct ttagctgctt caatcaattg tgccgctact
    45961 tctggacgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg gactgggcag
    46021 cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct catttctcta
    46081 aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaat
    46141 gttaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc tgagtttgta
    46201 aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg aaatacaaca
    46261 tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa
    46321 ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc aatctttctc
    46381 aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag cttaaaagat
    46441 gtttctgatt gagcaaaaaa aagactgtga caaaaatctt atcacagtcc attttccgga
    46501 ataaatggtg ttcaaaagct tgcatttgcg gtaataagtc caaccaaacg aaaaatagga
    46561 ggagctattt ttgtttggct gttcttatta cttgatgcag aacagctttt tcacaacctc
    46621 tccactaaac ggtgttcaat cagctgaccc tcggtattcg ctcaaaaaac aacaaaatat
    46681 gaggaactat tttcccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    46741 tcttttctaa cttctcaatc ctcttccttt attgatcaaa taccaagtaa cggcgtacaa
    46801 aacaacgatg aataacacaa gtacgatcat cgatgttgct actggcacat cgatcgttcc
    46861 aaggaatccg taacggaaac cggagatcat ataaacgatc ggattgactt ttgaaacagc
    46921 ttgccaaaag ggcggtaaca tagagatggc atagaaaacg ccacctagat aagttaacgg
    46981 ctgcaagaca aatgtaggca cgatcgatac atcatcataa gactgagcaa aaatcccgtt
    47041 gagcagtcct gctaatgaaa acaagattgc cgtcatcaat aatgtaatga tgaccatgaa
    47101 ccatgaatac acatgaagtg gtacaaagaa caaggaaatg atcgttacga gagagccgac
    47161 gagaatactg cgacccacac caccaatcac aaagccccaa ataatcacat gcgtaggaac
    47221 aggtgctacc aagatttctt cgatattttt ctgaaatttt tgagagaaga aggacgaaga
    47281 aacattggca tacgaactag tgatagcaga catcataatc aaaccaggaa cgatgaattc
    47341 catatatgaa aaaccaccca tgtcaccgat ccgcccaccg atcatttttc cgaaaataac
    47401 aaaatacaag gatgtcgtga tcactggcgg aaccagtgtt tggacccata tacgcaaata
    47461 gcggttcgtt tccttgatag ctaaactttt taatgcagta aaataaagat taaacatttt
    47521 tctcctccgt atgattattt tcttctgtga ttttcaagaa cagttcttct aaacgattcg
    47581 acttattcct catagaaaga actttgattc cttgtcttgt aagctgatcg aagattccgt
    47641 tcaccccttg atttcgttca acttcgacag cgattgtctg ctcatcttca aatgtacaag
    47701 catatccttc aatcacaggt ttggtatcat atggcgctaa atcaaagata aatgtttcaa
    47761 attgcagttt tgacagaagc tctttcatac ttgtattctc gatcagttca cccgattgga
    47821 tgattccgat gttgcgacaa agcatttcag cttcttctag ataatgcgtc gtcaaaatga
    47881 tcgttgtacc attttcgttc aattctcgta gaaactccca catctcgcgt ctcaactcga
    47941 tgtcaactcc tgctgttggt tcatccaaga tcagcaaacg tggttcatgc atcaatgccc
    48001 tagcaatcat cagtcttcgc ttcattcccc cggaaagcat acgtgcacgg acatttctct
    48061 tttcccaaag attggactgc ttcaaatatt tttcgctgcg tttcatcgct tctttgcgag
    48121 agacaccgta gtagcctgct tgattcacga cgatttgctg gaccgtttca aaagggttaa
    48181 aattgaactc ttgtggaacc aatccaatct gttgctttgc tcgaactaga tccttgtcca
    48241 gatcataacc gaagacattc acttttccag atgttttatt gactaaagaa gtaataatcc
    48301 cgatcgttgt agacttccca gctccatttg gccctaatag cgcatagaaa tctccttctt
    48361 tcaccgaaag atcgattccc cgcaatgctt cgaccccact tccatagatc tttttcaaat
    48421 tatttatttc aagcgcattt gccatttatt tcctatctcc ttatagacat gattcttaat
    48481 tgtatcatgg aatacagcag atgaaaaaac aacttggctt ccacacaaaa acacacctgc
    48541 taacaaagag gagatgtgtt ttctagttca tgatcatctt cgatagaata actatctaaa
    48601 caactctgga acttttaaaa tcataaaagt accgacagca actactacga tgacatatag
    48661 aagatcctta taactgaggt tcttgtcttt tttattcatg ttaagctttc ctccgtcctt
    48721 ttatcgaaag cttttttctt ttattttaac cgaaataaat aaaatggaaa tagttatttt
    48781 caatttctgt tttcgctgtt tgttttacgg agaactttgc catggaacgt gcgtctcgtc
    48841 gctgatgcta agttttttcg ataaaagctg ctagaaggaa gacagcgtgg tttattttgt
    48901 atcgtctttt gctaaatctt ccaagatcat ctggacagca gactcgttcc cattgaattc
    48961 tgccactttt ttggcatcaa tgtacttctt ttgataatcg tctttttgga cccacgcatc
    49021 atgcttcgtt tgataaagtt aatcaaacgg ataccactga cgctgttcat cgaacaacaa
    49081 actttctttt tgggcctgtt caactatgga tttaggataa ttcaaaactt tcagcaaggc
    49141 aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac tgattccttt
    49201 tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta aaatctctgt
    49261 caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa gccaccgaac
    49321 gactgaagcg gaagaagcaa tccgttcgat cgtatttgtt ccttttaaaa tctcatctac
    49381 gaagcagtag cagcgttctt tggaagccac ttggtccagc aaacgtttga tcgatttgat
    49441 ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca tggaagtcaa
    49501 cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg tctgtgctag
    49561 aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat tggaacccgt
    49621 gactaatgta ttcttctgcc agttgactgg attaactacc gcttctttta acaatggatg
    49681 atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag tgatcggcat
    49741 ataggtgcga taattcaaaa cggctgctgc aacttccaat tctcccagca attcccacag
    49801 cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat aggagataaa
    49861 tggcagcatg aacatgatgt tgacatattc aaaaatcaat tctgcttcac tcccgctctt
    49921 cattcgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt gctgtttcag
    49981 ctctttttga agtggtgtcg tcatttttgc taactgattg gcggctgaaa tcgttgaaac
    50041 caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta gataatagac
    50101 cgtattgaat actaagccac cgattgctag atagattcct agtacttgcc ctaaaagaag
    50161 caaacctaca cccaccagcg gaagcaagcc aagaaaaata aataaaccta cgcgtccaat
    50221 ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt tttttcctaa
    50281 acgagcgaat tgatattgga ttctttctct cagttgagga ttttcctgat aaaaagcaat
    50341 tagtttttct aactgataat cttctccaaa atcaaagcta cgtaaacgct gatacaacgc
    50401 ttctgaacct acactagaat aggtactgtt gatcccttca aatacttcga acatatccaa
    50461 gtcataccaa gtaagatcgt cgatttcact atcccatgaa cggaattttt tctccgtgag
    50521 ccacgcatcc ttcaaacttt cttctttatc aaatctcggt tgataaggaa tttttcccca
    50581 attactacga actgtttcct ttactttcaa acgtgaaaaa atgtctaaaa caactaataa
    50641 aataacaact gcaataataa ttaaaacgat aaattctctt tggcccatat ccctctccgt
    50701 tccttgattt gttagtttta gtatagcact gttgcgctat ttgaaaaggg ggtacaaaaa
    50761 taactgtctt cgttacctaa ttcgtatcaa tcacaccaaa ataaaaacac cttccaaaaa
    50821 agaagatgtc tttaaaacga gatacaattt tttgtcattc acttcaggta aattagagcc
    50881 aattaaaccc tccgcaactt gacggagatg gagaagatgc ccccgtctaa tctagcggtc
    50941 atctctcctc ccattccttc caccaatttt tttgacaggt aaagcccgat tccgctagat
    51001 tccgtatttg tgcggctggt attttcggta taaaaacgct ggaagacttt ttcaacagag
    51061 gtttgcgctc ctggtttgat aggattggcc actgttaatc gaatgaatga atcctctttt
    51121 gaaaatgtca cctttgcttg tccatctgca taacgcaata aattctgtat gatattttgc
    51181 agcactcgca gcaacatgag cggatctgct aatacagttg tatttatcga gtgttcagcg
    51241 aacgctacct cgatattttt ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc
    51301 acctctttag aaaaagaaag cgtatggaga tccatttctt tttgcttcgt ctctattcga
    51361 gcaagatcat aaaactgatc cgtcatactg agtaatcttg caacagattg tttgatgatc
    51421 gttaaatact tctcccgttt ctccgtatcc tctacctgtt ccagtaattg tacataaccg
    51481 ttaatagcag tcaaaggagt tctgagatca tgagaaaggc cgacgatcga tagtctgatg
    51541 ttttcttcca tttcttgaaa tgcttgtcct ttttgttcat tttcatccac ccatgtattc
    51601 agtgcatcta ctaggtccat tagattcttt tctcgaaatt cgactgaaag acgaccacca
    51661 tattcttgtc gttcagaaag agtggcgatt ttcttagtta atctcttgat ttccctttgc
    51721 aggaagaaaa taaaacttcc cataaagata acaacgagaa gcaacagcca cttaatcaaa
    51781 tctaaacccc actccccaca ccgtatgaat atacacagca tgtgcctttg ctagtttccc
    51841 tcgaagattg gaaatatgca tattcactgt tttctcacta tcaaaatatg gttcttgcca
    51901 aactgtttca taaagatttg ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt
    51961 cagcagatcg aattctcttc ctgtcaaatg gacagccaca ccattcacat ttacatccct
    52021 cgactctttg tccagtaaga tttccttgta atagatgcgt tgcgacagat cttttacttg
    52081 gacagaagtc tgatgacgaa gttggatgcc gattcgtaac aacagttctt cgatatcaaa
    52141 tggtttcgtc acatagtcat ccgctcctgc tgccaacaag gaaactttat ctctttgatc
    52201 gctttttgct gaaagaacga tgatcggaac attcgatgtc tgtcggattt ctgctaacaa
    52261 tgcttcacct gaaagccctg gaagcatgag atctagaagg atcaaatcga caggatatgt
    52321 gcttaacaat aattttcctt ccgtacctga ataagcatca agaagtttgt atctctgttc
    52381 aagaacctcc tccagtaaag aatgaacagc ttcatcgtct tcgataatca aaatagtttc
    52441 cagcaggaac acctacttaa tgtcagattt ttggaaaata aacattccta gagctgatga
    52501 taccacgatt gttactagac tgaccagaat cattttcggt gcttgcgaat aagttgcttg
    52561 ttgcgcaaag tccgtataca tgagccacgt ttccgtaaac cactccgtta gaaattccaa
    52621 gcctttgatt tttgcgaaaa caccgggaac gaaggccgtt cccatgatat aaagaaaaga
    52681 aagaagcaaa ctgacactgg tacttctagt gatgaatgtc aaagccactg gaacagataa
    52741 tgtcgctaat atcagcagaa gcaatacagg aaatactttt agaacataat ttccatagtt
    52801 tttgattccc tcaacttcac caaatccgaa agcaagtgtg taagcaatca tggctagccc
    52861 cgtaatcaaa accacgccaa gtgttgtgat caaaagcgta acgagaagtt tcgacacata
    52921 ataagtcatt cgattccgtc ctaaagataa cgtattgcga atcgtaccgt tttgaaattc
    52981 ttccccaata taaaagcacg caaatgctgc gaaagccaga agaatccatg cagccatttt
    53041 ttccaccatt aaaatccctg ttgcaccgtt gatgactgta tccgttgctt ggtttaattc
    53101 attgccgtat tttgtatgca tataccattc gttccaagaa taaaaaagcg gaagtacgac
    53161 catcagtcct agtgagaccc acattttacg gctcttccat acttttattt tttcagcagt
    53221 taatagatta aacatgtgga ttgcctccag ttaattgtaa gaaataatct tccagttttt
    53281 gcccaactag gtggatacca tccacaggaa cttttgcctc taccaatgct tgattgatgg
    53341 ctgctacttg gtcaattcct tcaaaaagct ggataacacg gctagattgg acaaaataat
    53401 ccctataacc aagagagtcg agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt
    53461 ttataaattg acgagattct tgtgccaatt cttctttcga taattctttg atcaagtgtc
    53521 cttcatgcaa aataccgtag tgagtggcga tctgtgacaa ttcatctagc aagtggctag
    53581 aaagcaagac ggtgatgccc cgttctttta ccaaacgttg gatgatttct cgcatttctg
    53641 ccatccctgc tggatccatc ccattcgtcg gttcatccaa aatcaaaaat tctggattcg
    53701 tgatcagtgc atttgcaata gctaaacgct ggcgcatacc taaagagaaa ttctttgctt
    53761 tctttttccc tgtattttcc aatcgcatca ttttcagcag gtcttcgatt tctctatccg
    53821 acacaccgcc gttcgctgct tggatacgta ggttatctct tgcagttaat tctggataaa
    53881 gagcaggggt ttcaatcgat tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc
    53941 caaataattc gatcgaacct tcatcaatcg agatcaaacc cataatagtt ctcataaagg
    54001 tagacttacc cgctccattc tccccaatca aaccatagat cattccacgt ttgatttcga
    54061 tcgatacttt atctaatgct ttggcagcac cgtatttttt ggttatacca gttgctttca
    54121 agaccgtttc atttccaaaa tccatactta tccttctttc ttattgataa gtgaagtata
    54181 gttgacgggt ataaagaaag cagccagaaa tgtataaaga aagtgtaaat tttccaattg
    54241 aatttcaaga aaaatcagac tgtctcgcag acgtggttga gttgtctgcc aatcaaagag
    54301 cgcaccaaac agtgctaatc caagtggact ggccaccatg gtaatcgttg ttatcgttga
    54361 catcagacgt ccttgtatat gcgttggaac ttctcgctga agccagacca tataaaaagc
    54421 aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa taatacatgc cgtttgggat
    54481 ttacaattag ctaccaaata agaggttatg acaaaagact tttcacaatc ccttcttccg
    54541 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatattta tcaaacatga
    54601 gaagcaattt ttgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
    54661 agaagcggat aaataatatt gtttgatgat actttatctt tttcccgttt gtttatagat
    54721 cgaatgatat tatagaccaa gaatcctacc tattaacata gctaaaaaca caaaaaaggc
    54781 actttccaaa ttgtggaaag tgccagaaaa gttaggatta aggtaactgc gctcagcatt
    54841 gcttcgccca gtacgattct cgtaactcgt gaactcgaac gataatcgca attaacgttt
    54901 tgagaactgt gaagctttac gggctttctt aagacctggt taaacaaaac tctattttat
    54961 tgcattttac aggaatgtat ttcatgcgaa attaataagt ctttgtttat agagttttac
    55021 tagggtataa aaagttgtat gttatcatta cctcaaaaca ttacctcagc ttggcgagtt
    55081 ggttgattga agaataaatc atttatctag atatctcaat aaaaaatgat ttctaagaaa
    55141 cttgtatttt cgtgttatta tttttttgcc ctatcaaaat ttctaattct tctagcgtaa
    55201 tatgattatg acacataatc gattcaatta cttcaatacc tatttttttc gagaaagatc
    55261 ccttttgttc actcacaaaa atactttcag aagacatata taaggaacga agttttggaa
    55321 ttagtatatc gtacaaatag accaccataa aatcgtcctt gcaaccattc tcaaaaagaa
    55381 ttaaatttgc tgattgtgtt acatatatca ctgaattaca ttcatggtca attccaaaaa
    55441 caatgtctac ttcattttct tttctaattc gcgcagttac atttttcaaa ctttcaccat
    55501 tacttataaa tgctttagtt actgtaagct ctgatgtttg ttcagatatt ccttttataa
    55561 tattttgttc atcagccaca ttccccataa atccgccgat tccttttaag taataattct
    55621 cacctaagag tcgcttgcat tcactgaatt gataaaaaaa ccagttaaaa aaatcttctg
    55681 gaaaaataaa ttctgatcga actttattta tatttttagc tcctattaat tttataactc
    55741 gattaatgtt cacttgctgc gatgaacata taactgaata aattgagttg tttaattcaa
    55801 aaaaacaact ttaacatatg catcattaat tcgttatttt ttaggtagat attgatcatt
    55861 gttatctctc tttttttgct tcccatactc caacttagaa attgccgtta taaaatttat
    55921 tttaaaggtg actttttccc ctgaagtatc ggtagtagga atacttaata cctcagcttc
    55981 tgaggaggta tcccaattat agttatactt tgcatcatca ccataaagaa caattccatt
    56041 ttttttatct tgtgccgtta gctttactgg ttcgcttact tttcttgtta atatcttgga
    56101 ctttacatca tcgatattgc ttattttatc aagcttaaat aatttacaaa ttaatttcgt
    56161 actagttttt atatttg
//
LOCUS       DAFCNS010000008        55809 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFCNS010000008 DAFCNS010000000
VERSION     DAFCNS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.008"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            139..996
                     /locus_tag="LNE23_000479"
     CDS             139..996
                     /locus_tag="LNE23_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBK6930356.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1111..2031
                     /gene="pstC"
                     /locus_tag="LNE23_000480"
     CDS             1111..2031
                     /gene="pstC"
                     /locus_tag="LNE23_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBK6930357.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2031..2915
                     /gene="pstA"
                     /locus_tag="LNE23_000481"
     CDS             2031..2915
                     /gene="pstA"
                     /locus_tag="LNE23_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBK6930358.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2930..3736
                     /gene="pstB"
                     /locus_tag="LNE23_000482"
     CDS             2930..3736
                     /gene="pstB"
                     /locus_tag="LNE23_000482"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK6930359.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3752..4510
                     /locus_tag="LNE23_000483"
     CDS             3752..4510
                     /locus_tag="LNE23_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK6930360.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4523..5200
                     /gene="phoU"
                     /locus_tag="LNE23_000484"
     CDS             4523..5200
                     /gene="phoU"
                     /locus_tag="LNE23_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBK6930361.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5438..5737
                     /locus_tag="LNE23_000485"
     CDS             5438..5737
                     /locus_tag="LNE23_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930362.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5763..6470
                     /locus_tag="LNE23_000486"
     CDS             5763..6470
                     /locus_tag="LNE23_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK6930363.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6467..7234
                     /locus_tag="LNE23_000487"
     CDS             6467..7234
                     /locus_tag="LNE23_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK6930364.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7334..8902
                     /gene="liaX"
                     /locus_tag="LNE23_000488"
     CDS             7334..8902
                     /gene="liaX"
                     /locus_tag="LNE23_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBK6930365.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9199..9519
                     /locus_tag="LNE23_000489"
     CDS             9199..9519
                     /locus_tag="LNE23_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBK6930366.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9519..9869
                     /locus_tag="LNE23_000490"
     CDS             9519..9869
                     /locus_tag="LNE23_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK6930367.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10036..10974
                     /locus_tag="LNE23_000491"
     CDS             10036..10974
                     /locus_tag="LNE23_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBK6930368.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10989..11819
                     /locus_tag="LNE23_000492"
     CDS             10989..11819
                     /locus_tag="LNE23_000492"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBK6930369.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11863..12888
                     /locus_tag="LNE23_000493"
     CDS             11863..12888
                     /locus_tag="LNE23_000493"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK6930370.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12903..13841
                     /gene="galU"
                     /locus_tag="LNE23_000494"
     CDS             12903..13841
                     /gene="galU"
                     /locus_tag="LNE23_000494"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK6930371.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13966..14406
                     /locus_tag="LNE23_000495"
     CDS             13966..14406
                     /locus_tag="LNE23_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBK6930372.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14409..15011
                     /locus_tag="LNE23_000496"
     CDS             14409..15011
                     /locus_tag="LNE23_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK6930373.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15079..16185)
                     /locus_tag="LNE23_000497"
     CDS             complement(15079..16185)
                     /locus_tag="LNE23_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK6930374.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16395..17396
                     /gene="ccpA"
                     /locus_tag="LNE23_000498"
     CDS             16395..17396
                     /gene="ccpA"
                     /locus_tag="LNE23_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBK6930375.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17732..20077)
                     /locus_tag="LNE23_000499"
     CDS             complement(17732..20077)
                     /locus_tag="LNE23_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK6930376.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20356..21258
                     /locus_tag="LNE23_000500"
     CDS             20356..21258
                     /locus_tag="LNE23_000500"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBK6930377.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21471..23246
                     /locus_tag="LNE23_000501"
     CDS             21471..23246
                     /locus_tag="LNE23_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBK6930378.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23503..24720
                     /locus_tag="LNE23_000502"
     CDS             23503..24720
                     /locus_tag="LNE23_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK6930379.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKEALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(25167..26753)
                     /locus_tag="LNE23_000503"
     CDS             complement(25167..26753)
                     /locus_tag="LNE23_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBK6930380.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            27004..27228
                     /locus_tag="LNE23_000504"
     CDS             27004..27228
                     /locus_tag="LNE23_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBK6930381.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            27370..29121
                     /locus_tag="LNE23_000505"
     CDS             27370..29121
                     /locus_tag="LNE23_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6930382.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            29121..30905
                     /locus_tag="LNE23_000506"
     CDS             29121..30905
                     /locus_tag="LNE23_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6930383.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            31363..32214
                     /locus_tag="LNE23_000507"
     CDS             31363..32214
                     /locus_tag="LNE23_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6930384.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            32311..34197
                     /locus_tag="LNE23_000508"
     CDS             32311..34197
                     /locus_tag="LNE23_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6930385.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(34238..35140)
                     /locus_tag="LNE23_000509"
     CDS             complement(34238..35140)
                     /locus_tag="LNE23_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6930386.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            35370..36773
                     /locus_tag="LNE23_000510"
     CDS             35370..36773
                     /locus_tag="LNE23_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK6930387.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            36770..38206
                     /locus_tag="LNE23_000511"
     CDS             36770..38206
                     /locus_tag="LNE23_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK6930388.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            38351..39277
                     /gene="trxB"
                     /locus_tag="LNE23_000512"
     CDS             38351..39277
                     /gene="trxB"
                     /locus_tag="LNE23_000512"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBK6930389.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            39490..41217
                     /locus_tag="LNE23_000513"
     CDS             39490..41217
                     /locus_tag="LNE23_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBK6930390.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            41233..41583
                     /locus_tag="LNE23_000514"
     CDS             41233..41583
                     /locus_tag="LNE23_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK6930391.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(41667..42527)
                     /locus_tag="LNE23_000515"
     CDS             complement(41667..42527)
                     /locus_tag="LNE23_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBK6930392.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            42619..43047
                     /locus_tag="LNE23_000516"
     CDS             42619..43047
                     /locus_tag="LNE23_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK6930393.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            43209..43385
                     /locus_tag="LNE23_000517"
     CDS             43209..43385
                     /locus_tag="LNE23_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBK6930394.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            43412..43858
                     /locus_tag="LNE23_000518"
     CDS             43412..43858
                     /locus_tag="LNE23_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBK6930395.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(44014..45393)
                     /gene="brnQ"
                     /locus_tag="LNE23_000519"
     CDS             complement(44014..45393)
                     /gene="brnQ"
                     /locus_tag="LNE23_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK6930396.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            46099..47070
                     /locus_tag="LNE23_000520"
     CDS             46099..47070
                     /locus_tag="LNE23_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBK6930397.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            47094..49286
                     /locus_tag="LNE23_000521"
     CDS             47094..49286
                     /locus_tag="LNE23_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBK6930398.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            49303..49779
                     /gene="ybeY"
                     /locus_tag="LNE23_000522"
     CDS             49303..49779
                     /gene="ybeY"
                     /locus_tag="LNE23_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBK6930399.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            49763..50158
                     /locus_tag="LNE23_000523"
     CDS             49763..50158
                     /locus_tag="LNE23_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBK6930400.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            50173..51072
                     /gene="era"
                     /locus_tag="LNE23_000524"
     CDS             50173..51072
                     /gene="era"
                     /locus_tag="LNE23_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBK6930401.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            51215..52027
                     /gene="recO"
                     /locus_tag="LNE23_000525"
     CDS             51215..52027
                     /gene="recO"
                     /locus_tag="LNE23_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBK6930402.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            52411..53328
                     /gene="glyQ"
                     /locus_tag="LNE23_000526"
     CDS             52411..53328
                     /gene="glyQ"
                     /locus_tag="LNE23_000526"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBK6930403.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            53330..55405
                     /locus_tag="LNE23_000527"
     CDS             53330..55405
                     /locus_tag="LNE23_000527"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBK6930404.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        18080 a         9980 c        11845 g        15904 t
ORIGIN      
        1 atagcataga aacttttaat gagatagttt tttttacaat tatgagaatt tattttgtta
       61 tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa catactttag
      121 gattaaagga gatttctaat gaaaaaactt aaattattct taccaataat aggattagcc
      181 attcttttat caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc
      241 tcttctgctt tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct
      301 ggaaaattca tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca
      361 ggagcagtcg acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc
      421 tcagcattag tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat
      481 attggcgtaa aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa
      541 aactggagtg aagtcggtgg aaaagatcag ccagtagtta tattgaaccg cgcttcagga
      601 agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca
      661 caagaacaag attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc
      721 agctatgttg ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta
      781 gaaccgacag atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg
      841 tacacaagag atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat
      901 gaagtacaag ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc
      961 gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt
     1021 agctaaactt ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt
     1081 atgttaaacc tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca
     1141 aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg
     1201 ctgatcgtgt tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc
     1261 ttttttactg ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc
     1321 caaatcggag caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt
     1381 attgtgacct ttttatctgc tatcgttgcc acgccatttg ccattggtgc agcagtattc
     1441 atgacggaga tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt
     1501 gtaggtattc cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc
     1561 agaagtattt ttggcgggac agggtttggt atcttagctg gtacatttgt gttgttcgtt
     1621 atgatcttgc cgactgtcac aacgatgact gttgatgcat taaaggcagt gccacggcac
     1681 tatcgggaag cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg
     1741 ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt
     1801 ggtgaagcct tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggactt
     1861 actactcctg cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg
     1921 ggaacagttg aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta
     1981 ttctttaata tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa
     2041 aacgctcaga taaaattgct acaggtgttt tatatatagt ttcggggatc atcgtattga
     2101 ttttagcagc tttactactt tatatcttag ttcggggaat accgcatatt tcatggtcct
     2161 tcttgaccga accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta
     2221 actcgattta tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg
     2281 gtatctatct ttcagaatat gctggaaaaa actggctaac agatgtgatt cgcacgtcga
     2341 ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg
     2401 ttattcaatt ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca
     2461 acttgcctct tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac
     2521 gagaagcagg attggcctta ggtttgtcac gctgggaaac agtgataaaa gtggtggttc
     2581 cagaagcgac accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg
     2641 aagcagctgc attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact
     2701 ggaatccatt aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg
     2761 tccatatttg gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg
     2821 gcgcttctgc cgtgttgatc attgctgttt tactgttcaa cttcggcgct cgtgcgattg
     2881 gcaaacgatt ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata
     2941 taactggaat gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac
     3001 ggatgatcta catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt
     3061 tgaaaaaaat aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct
     3121 tcgttcgttg aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat
     3181 gtatcaaggc gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat
     3241 tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt
     3301 tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag
     3361 cttgaaacaa gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc
     3421 tttatctggc gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga
     3481 tatcttgctg ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga
     3541 agaaacactt gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca
     3601 acaagcagct cgtatcagcg actatactgc ctttttctat ctaggaaaag taatggaata
     3661 tgacaaaact aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc
     3721 cggtcatttt ggttaagacg gaggcctcaa catgacaaaa gaaattattt catctaaaga
     3781 tcttcatctg tattatggaa agaaagaagc actaaaaggg atcaatctaa gtttcaatca
     3841 aggggaaatc actgcgatga tcggtccttc tggttgcggg aaatccactt atctgcgttc
     3901 tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc accggaagtg ttttatataa
     3961 agacaaagac atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat
     4021 ggtattccag cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt
     4081 gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa
     4141 ggcagcgtct gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc
     4201 cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat
     4261 cttattggat gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat
     4321 gctgttagaa ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc
     4381 ttcacgtatt tcagataaga cggcattctt tttagatggt cacttgatcg aatacaacga
     4441 tacgaagaag atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg
     4501 atttggataa ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct
     4561 ccataatcag ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg
     4621 tgcatacatc aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat
     4681 caataacatg gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc
     4741 agttacaact gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg
     4801 tatggccgat catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag
     4861 aataccggaa atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga
     4921 caacgtccta gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat
     4981 ggatcaaaga gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca
     5041 ggaaaatcct gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga
     5101 acggatcgga gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa
     5161 aatcactgag ttaaatacga accacaatga gattttctaa actgaagctg tgattatgcc
     5221 gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag
     5281 taataagaac agtcaaacaa aaaatagctc ctcatatttt tcgtttggct ggacttatta
     5341 ccgcagatgc aagcttttga acaccgttta ttcgggaaag ggtttgtgac aaaacatgtc
     5401 acaaaccctt ttcatcattt catagaaagg ataaagaatg caaaaaaaaa ttagcatcat
     5461 cactatattt tttatttgtg tgcttacttc atgggttatt cagcatcaag ctgctttata
     5521 tgaagtacct gtcatcgaga tcgaaaaagt acgaataaaa gaggatcatc aattgattac
     5581 aggaagactg atgaacacgg atcaaaaagg agaatcgatt tatttatcgg ttccttatca
     5641 aaaaaatcaa atagaaacag caaatataaa agcgagacaa atgtatctag tccaaatgca
     5701 tggagatgaa tggcagctag tagcaaaaaa aaaatgatgg ctggcttttc ttcttcacaa
     5761 gtatctccat tggcgccatg ttgcttattg gcggaaaaca gggtctattt gctttaggaa
     5821 gtctcatcat caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag
     5881 ggatttctct attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat
     5941 tggctttaga tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg
     6001 tttttttagc attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc
     6061 gctatgagga actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac
     6121 tgattggaac agctggagga acactcgata ccgttatcac agtcattgcg acattagaag
     6181 aattgactaa gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag
     6241 tgggtcgaga tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt
     6301 cagttccatc gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac
     6361 agcatttatc attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga
     6421 tccctatctc ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat
     6481 tatacttgtt gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta
     6541 tattctttta ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca
     6601 tcagaagtgg ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat
     6661 cttatttttt atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat
     6721 ctttttattt tgtttcctac tattgcttcc tatcactgat tttttagcga tccaaggttt
     6781 tacatcggta gaactagaag aattatctgg cttagataag actttagcga ttgattttag
     6841 attacttacg agatcgttgc ttctgattag tcttagtggt gctgttttag atgctagtgt
     6901 agcaatttct agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca
     6961 attgcgccat tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact
     7021 tctttttgca ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat
     7081 tccttttcag caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct
     7141 taccacattg tctgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt
     7201 tacaaaaaag aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat
     7261 ttttactgaa tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa
     7321 ggaggcaatt tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct
     7381 ttcgactgaa gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca
     7441 aataaaaaaa gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca
     7501 attgatcgat aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga
     7561 aagagaaagg caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc
     7621 gaataaaact tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc
     7681 acgcaatgag aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt
     7741 gacagaagaa gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc
     7801 tagtggcgat acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg
     7861 taagaatcaa tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga
     7921 ttggaaagat caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg
     7981 atcacagtta ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga
     8041 caacatggaa tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga
     8101 gcatgaattt tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg
     8161 aaacgtgacg ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact
     8221 ttacggaaaa atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt
     8281 taatgaagat catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt
     8341 ctacttacct aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat
     8401 gattgaaaca ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt
     8461 ggaccaatta actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg
     8521 aaacgggaac atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc
     8581 gacagcagaa aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga
     8641 agataatttg aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc
     8701 agatggcatc ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct
     8761 gtccaattac gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg
     8821 tcgtgtttca gatggtgaaa tcacgcaagt gcaactttca acgacaacag gaagtatcta
     8881 tttaaaagat acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag
     8941 aagcgatttt gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgataggc
     9001 tatctcttga acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa
     9061 ggaataattt cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat
     9121 tttgcagaaa gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata
     9181 gtagaacaga ggtgagacat gatgaaaaaa aagttgacaa aatccaataa aaatgttgtt
     9241 ttgacaggga ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt
     9301 gtgctttatg tattcctgag cttagttgct tttggaagtc caatcatttt atacatttta
     9361 ttagctttga ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca
     9421 tattatgaaa aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa
     9481 gcggaaaaaa tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt
     9541 tagttaatac gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga
     9601 gcagtatttg gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca
     9661 aaccgatttt gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt
     9721 ttgttatcaa tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta
     9781 gtttttcttc attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa
     9841 ttgtcagtga acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt
     9901 tttttattta gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt
     9961 ttttaattgg tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga
    10021 aatgaggtag catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct
    10081 ttggaaatcg tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc
    10141 tcacgcccag gactagagtt gactggttat tttaattatt attcccataa tcgtttacag
    10201 ctatttggca gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc
    10261 atcatgagaa gattatgtag tgaagatatg cccgcattta ttatttcaag aggattaccg
    10321 gctccagaag aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc
    10381 atctcaactt ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct
    10441 cgaacaagtg tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt
    10501 gacagtggga taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg tcatcgattg
    10561 atcgcagacg atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg
    10621 cctaaaatcc tggaacatct aattgaaatc cgaggaatcg ggatcattga tgtaatgaat
    10681 ctttttggtg cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa
    10741 gcatgggcaa aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc
    10801 gcggaagtgg gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc
    10861 atcattgaag tagccgcgat gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa
    10921 acatttgaag aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga
    10981 gtgacaatat gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct
    11041 actggtatgc attgatcatc gtatcaggta tcattatcgc catgtggctt agcagcagag
    11101 aggctgttcg ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc
    11161 ctatctccat tattggtgct cgcttatatt atgttttatt tgatcttccc cagtatatag
    11221 ctgatccaat ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga
    11281 ttgcaggcgc gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt
    11341 ttttagatat cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt
    11401 tcatgaacca cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt
    11461 taccgcaatt catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc
    11521 tttatgaatc actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac
    11581 cacacctttt gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc
    11641 gtttctttat tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt
    11701 ctcaattatt atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa
    11761 aaaaaggaaa tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat
    11821 ttttagagcg ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg
    11881 gttttaggac caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat
    11941 gacgttcgga tttggggaca tcgtgcttca caagtagaag aaatcaacac ccaacacacg
    12001 aatcaacgtt atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg
    12061 gaagatgcag taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt
    12121 tctgttgcaa aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt
    12181 aaaggattag aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca
    12241 gcagaaaaaa gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagcg
    12301 aaacatgaca tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta
    12361 caagagctgt tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta
    12421 gagatggggg cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta
    12481 gggtttggcg ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt
    12541 ttaggtgtag caatgggtgc caacccgctg acctttatcg gattaagcgg agtaggcgat
    12601 ctagtcgtta cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga
    12661 caagggcagc ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat
    12721 actactcaag cagcgatgga actagcccaa ataatggaaa tcgaaatgcc catcactcaa
    12781 acaatctatg aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg
    12841 ttacgtgatg ggaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt
    12901 atatgaaagt caaaaaagca gttattccag cggcaggatt aggaacaaga tttttaccag
    12961 caacaaaagc tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca
    13021 tcgttgaaga agcgttgaaa tcaggaatcg aagacatctt gatcgttaca ggaaaagcta
    13081 aacgaccaat cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa
    13141 aaggaaagac cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca
    13201 tccgtcaatc tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg
    13261 gaaatgaacc gtttgtcgtt atgctaggcg atgatatcat ggaagatgaa gtaccattga
    13321 cacagcaatt aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag
    13381 ttcctcatga tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg
    13441 ggttatacaa tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt
    13501 tagctattat tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac
    13561 aaccaggagc cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac
    13621 aacgcgtatt tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt
    13681 ttatgaaaac aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg
    13741 agtatatcct gtctttaggt aaaaaattag aagcagaagg aagcaagatc gatcataatc
    13801 caaaaggaat aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct
    13861 tcagctgcta attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga
    13921 aaatgctatc cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa
    13981 attgcaggat tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg
    14041 ttgttgcaag tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg
    14101 acgatcgaaa tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttgctg
    14161 gtcaaaagta atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg
    14221 ttcacagcag ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac
    14281 atcgcgacga aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa
    14341 gtcggtggga cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga
    14401 aaataaaaat ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggaacagccg
    14461 cagcaatcgc cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag
    14521 ctgcccaagc ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa
    14581 aatcgactga gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag
    14641 gtgatctagc aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg
    14701 aatctgacga tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca
    14761 aaaaaacggc agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata
    14821 cttcagaaga tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca
    14881 atgaaggaat gtcagaagca aaaccagttg cagatgacac aaaagaaatg atgaaaatgg
    14941 caaaagaaga tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta
    15001 caaatcacta aaatcactaa aatcacaaat ttatagaata aaaaggtggg agcggactag
    15061 ctctcacctt ttttactctt atttttctaa aactagtaat tcttttggtg tagtcgtgaa
    15121 tggcacacag ccatcgcttg tcacatatac acagtcttcg atacgtaccc ctactttttc
    15181 aggaatatag attcctggtt caagagagaa gcacatccct tcttcgatta ctaagtcatt
    15241 tccttctaca agggaaggga attcgtggac ggttgttccg atcccgtgac ctaaacggtg
    15301 attgaaatat tcgccgtagc catagctgtt gatcacgttt cgtgcgattt gatcaagttc
    15361 acctgctgtc acacctggac ggacggcttc ttgtgcggca agctgtgctt caagagtgat
    15421 gttgtatatt ttttcttgga agtcggttgg tttttgataa gcaattgtac gagtagcatc
    15481 actgcaatag ccattccaaa caacgccaag atcgaataac acaagttcat tcggtgaaat
    15541 cttcgtgttt cctggtgtac catgagggct tgcagcattt gctcctgcaa ggactaatgt
    15601 gtcgaaagac atttgtgaaa caccttgttt tttcaattgg tattcgattt ctgcaatgat
    15661 cgcttgttca gtcacgcctt ctttcactgc tttaaagcca atctcaaatg caacatcggc
    15721 ccatttacca gcttcgatca gtttgtcttt ttcctctaga gttttgatca gttgtagacg
    15781 ttggatgatg gaagctagat cagtgtaaga atccgttccc gtcaagatat cagagatagc
    15841 atcgaagcgc tcgacagtca aggcgttctt ttcaatccct acattgccag gctgaccata
    15901 gcgagcacga agttcttttc cgatgatctc ccaagggttt tcgctgtcta aatagccacg
    15961 gacatcatac gcccaactac tgttttgagc atcttcaact tccaaagcag gagtgaaaag
    16021 gaaaggatct tgttcaagag ggataaaaag agctagtaca cgctcatgag gatcactttc
    16081 gtatccggaa aagtaagcga tgtggccagg gtcgctgata taagccaatt cgacttggtg
    16141 ttgtgccatc cattgacgta gttcattgat ttttacgtta ttcatttggc aacctacttt
    16201 ctaaattaat tttgtattca ataattgtac cactgattta ttaaaagacc aacgttctaa
    16261 aaactgaaaa aagtaaaaat tttcatgaaa acagagataa aacggttgca attcttgaga
    16321 ttatttgata ttctatttta gaaatgtaag acgtattttc agaaaacata taaaatatga
    16381 ataggagaac acaaatggaa aagcaaacaa tcactatcta tgatgtagcg agagaggcaa
    16441 atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtc aaaccagcaa
    16501 caagaaaaaa agtcttggaa gttatcgatc gcttggatta tcgtccaaac gcagttgcgc
    16561 gtggcttggc aagtaagaaa acaacaacag ttggggtaat catccctgat gtcagcaata
    16621 tgttctttgc ttctttagca cgtggtatcg atgatgtggc aacaatgtac aaatacaata
    16681 ttatcttagc aaattcagat ggtaacgacc aaaaagaagt caatgtattg aataacttac
    16741 tggctaagca agtggacggc gtgatcttca tgggtcacca tatcacagac gaaatccgtg
    16801 gagaattttc acgttcaaaa actcctgttg tattagctgg ctctattgat ccagatgaac
    16861 aagtaggtag cgtaaacatt gattatgtat cagcaacaaa agatgcagta aacaaattgg
    16921 caaaaagcgg caataaaaaa atcgcttttg tcagtggtgc gttgatcgat cctatcaatg
    16981 gacaaaatcg tctgaaaggc tataaagaag cattaagcga aaataaccta ccatatagcg
    17041 aaggattgat ctttgaagca caatacaact tcaaagatgg tctatctcta gtcgatcgga
    17101 tccataatag cggtgcaaca gctgtctatg tttcagatga tgaattagcc atcggtattt
    17161 tggatggttt attagaccgt ggcgtgaaag taccggaaga attcgaaatc atcacaagta
    17221 acaattcact actgacagaa gtagctcgtc ctcgtttaac aagtatcaca caaccattat
    17281 acgatttagg ggcggtatct atgcgcctat tgacgaaaat gatgaataaa gaagaagtcg
    17341 aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa aggatctaca aaataataaa
    17401 aggacaacca ttcagaggtt gtgagcctgc cgtttaactc tgcgttacaa ggagcaattt
    17461 caaaaaacag catttcatgt ttgatgaata ttgtgactcg tgattcggag ttggcaggag
    17521 aacaccgttt attcgggaaa aggggttatg acaaaatttt gtttaatcct ttttttttgt
    17581 cttaaaaaag agagaaagtg gttgaaatta catttatcgt atataaaaca atccgtgtat
    17641 agatgctgca ttatgttcac caccatcaaa aaaataaggg actgtgacaa tactattgtc
    17701 acagtccctt attactgtgt attctattaa atcaatcatc tttctcatct gattctttgg
    17761 ttgcttgatt cgctcttgca taagctgaag cggtgttcca gtaatctgta tagttttcat
    17821 cggtaccgcc aacgccaaat ttaaaggcgc ttttcttcgg tccatttttc gcccatagcg
    17881 attcaactgt ttttcctggt gtttggatgg attttttatt gacagtaact tttccaggtg
    17941 tttgaccagt aaatccagat acttttttct tcatcacatc agatgataat tcgaatttct
    18001 catcagtgcc aaaaatgtca ggttttgctt ggtaagcttg attgatcaga ttggcaagat
    18061 aattagccga acgtatcctt gctttgctgt ccattccagt attgtcatcg tgccctgccc
    18121 agctactgat ggtaatagac ggagtagaga caattagcca tgaatctcgg tattcattgg
    18181 tagaaccagt ttttccgacc caatctgctt ttcctagatt tccgtttaag cttgagatgg
    18241 catctttgaa tggtgtagtg atttttgcat tgatcacact gcgcatcatg tcattcatga
    18301 tcgatgccgt tgcttccgaa tagatccgga cagagttgct ttcgtgttta taaattacat
    18361 ttcctgcgtt atctgtgatg gaatcgatga tatagccttc ttcatagacg ccgccgtttg
    18421 ctaaagcctg aaatccattg gtctgttgaa gcgtcgtgac atctactgta cctaaaggag
    18481 cagattcaac gccccaattg tcatttgccg ggtagttcat ctttgccaag tagtgctcgt
    18541 aagcatattg tttcgatcca ccggaattca acacatcttg atataagtga taagccggga
    18601 tattgttgga ccattctagt gactcacgga cggtttggaa ggtgttcgat ccttcgttag
    18661 tagcattgac gattttctcg ccagcatttt ctccttcttg ccaagttgtt gcataatctg
    18721 atacacggga ttctgatccg ataagcccca tgtcgattgc aggtccataa acaagaactg
    18781 gtttgattga tgagccagct tgacgttctg tatcgaaggc atggttattt tgattctgac
    18841 tgtaattccg tccgccaacg aatccgtaga tgcggccagt tctgttatcc atcaatacat
    18901 ttccggtttc tacttgactg ccagcaccat catctaacat gtatccataa ttttctacgc
    18961 cattttgcaa tgcagcatag acatctttat cgattgtcga atggattgta tatcctttat
    19021 tttggatcgt ttgctgtgcc ttttgataat aagcgtcact gaccgatgaa tcagacatat
    19081 cagcgttgtc tttttcagcc aattgttgtg caatgattct tgttgcttca ttcatcacag
    19141 tataatacaa gaattctctg tcagtctgtt ctgcgatttg ttgtggtaga aagtcttttg
    19201 taaggtcata ggcttttgct tcttcgtatt gttctttcgt aatttggcct tcacgataca
    19261 tactgaataa aacaaaatct tttctggcaa ggccggcaga aagatcttct tttagcgcac
    19321 ctgtgttcgt gtatggacta tacgtgatcg ggctttgggg taatccagcg atataagcag
    19381 cttgcggcag cgtcacatct tttgcatcta caccaaagat tcctcttgca gcttcctgga
    19441 ctccagcaat gttttgtccc ttattattac gaccaaatgg tgagacattc aaatacgtag
    19501 caatgatctc atcttttgaa aagtattttt ctacttgtgc agaaagaatg atctcgtttg
    19561 ctttacgttt gaatgttgtt tcatctgtaa ggatctgctg tttgactaat tgctgtgtca
    19621 aagttgatcc accagaagag ccaataccag tagcttcaga tactaatgct cgtaaaacag
    19681 ctttaggaac gtaaccttgg tgtttatcga agtattcatc ctctgtgctg ataattgcag
    19741 ttttcaataa aggtgaaatt ttatcagatg aaatagtcgt gcgcatcaga tctgtctgga
    19801 tttttgatat ctctgtatta tctgcataaa caatctttga cgtttctgta atgtttccta
    19861 gttctgtctg cagtgctttt ttagtcggca attgtgtgtc ttcgacgaga taggcaaaat
    19921 agccagcgcc tattccaata cctagagctg cgaacatcaa aagaagaaca gtaataaaaa
    19981 cagtcaatga ttgaaaaact ctaaaaataa tcttaggaac aaaccatttt tctttttttc
    20041 ttctattctg tttttttcga gtatttttgc gtggcaaaag ggtcacctca actaatggtt
    20101 ttatttcgct aattatagca aataatagaa caaaaaaaca gacaaggctg tttttggcag
    20161 gaatttcttt tttaaaagag ttaaaacgtg agaaaatgat cttttttata aaagagttct
    20221 taagcttttc tttagagaaa atttcagtta ctatggagaa cagggcaaag aaaaatcaat
    20281 cattttcttt gtatatcatc ttaccaatat ataggaattg ttagtaacaa ttgcctttca
    20341 aggaggaatc catccatgtt attaggctca cacgtcagca tgagcggaaa gaaaatgctg
    20401 ttaggagcag cagaagaagc agccagttat ggatcttcga cttttatgat ctatacaggc
    20461 gcccctcaaa acactagaag aaaagccatc gaagagatga atattgatgc cggaaaagcg
    20521 tacatggcag aaaacggtct aacaaatatc gtcgttcatg ccccttatat cattaatttg
    20581 gggaatacga tcaaaccaga aatgtttcct tttgccgtcc aatttctgag agaagagatt
    20641 gttcgtgcgg aagctttagg cgctactcaa attacacttc acccaggtgc tcatgtaggg
    20701 gcaggaacag aagccggaat tgctagaatt attgaaggct tgaatgaagt tttgacaaaa
    20761 gaacagacgg cacaaatcgc tttggagaca atggcaggta aaggtacgga aatcggtcga
    20821 acatttgagg aattagcgca aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt
    20881 acttttgaca cctgtcatac gaatgatgcc ggttataata ttaaagaaga cttttcaggt
    20941 gtaatggaag agtttgataa gatcatcggg ttagatcggc ttaaagtact ccatatcaat
    21001 gattctaaaa atccgcaagg ttcgcataaa gaccggcacg ccaatattgg ttttggaaca
    21061 atcggattcg acacgctgaa taagatcgtc catgatccaa tgtttgacaa tatttcgaaa
    21121 atcttggaaa ctccctatgt cggcaaagat aaaaagaaag cttacgcacc atatggaaaa
    21181 gaaatcgcca tgttcaaagc acaaatattc gatccagaag tgtttcctga gttattagca
    21241 gaagaagtga tactctaaga ttttctatct gacagcagca aaaaaggacc aggcacattg
    21301 ccttgtcctt ttttgtttaa gaaacaaagg agtgcttttt gcagtctttt tcatcatgca
    21361 tcttctcccc cctcttttta aatttagaca aacagggtat actggtagaa aagtaaaaaa
    21421 acgaaaagtt tcacaagcaa gtggcaaaac gtgattggaa ggacgaaaag atgctgaaca
    21481 ctttaaaacc cttgatcaac caactaaaaa agaaacatac atactttatt cttggcatag
    21541 ccctattatt tacaattatt cgtttgtatt tatatagtaa agcaatctat ggaacagaca
    21601 tgtcggcaac ctatgatgac cagctgctga ttcgctatgc tcaaaatatt ttggacggta
    21661 aatggctagg ggagtatacg actacaacac tttccaaagg aatctcctac agcttatttt
    21721 tagtacttgc acataaactg catctttctt atgcagtttt gttcggcatg ttcagtattt
    21781 ttgccagtat attggttgca ctcgcgttta aaccgatcgt gaagaacaag tatcttcttt
    21841 taagtttcta tttttatttt ttgttttctc cgattagttt tacacatgaa tattccactc
    21901 gaacgtatcg taatgccctc gtgattccgg ctgttgagat cattatcgct tgtttactag
    21961 ctatttatta ccgtaaatat agttccagca aaaaaatgct gccgtggttt ttagggctag
    22021 ctgtttcttt tcctttcttt tggttcattc gcgaagattc catctggctt cttcctttta
    22081 ctgtagtcgc gttaggcatc agtttcgtcc agatcttgct aggacaagac ttttctttca
    22141 aagaagcaaa gaagttaaaa gaacgaccat ttctcctttc gaaacaagaa tggctgaaac
    22201 tttcattgtt tttactccct ctagtcggtt tctccatcgt ctatcagtgg atcgaatata
    22261 aaaacgagac aacatatagt attgccgcca tcaatgaccg ttcgagtggc gaatttggac
    22321 gtttgatgaa acagctgatc cgaatagatg acgaaacgaa cttaaataaa gaaaacagtg
    22381 agatatgggt ctcgcatgct gcattaaata aagcattaga cgcatctcct acatttcatg
    22441 ctgtttctac tcgtattgac gaattgtata cgactcatgc gtggacccaa ggtggaaaag
    22501 taaaagaatt accaggagat atcatttttt gggcattgcg agatgtggta agcgaatctg
    22561 gttattacca aaacaatgca aaagaaacca atgatttttg gaaaaaagtc aatcaagaat
    22621 tagaagaagc ctatcaaaag ggaactatca aaaagaaaaa agaattctat ctgatgggat
    22681 caggggatgg aaagacgatg aatgatctgc caatcgtatt tgcttttctg aaatcaggat
    22741 atgcctatgc atggcgatac aaagatttcc atcaaggggg gaattatagt ttcggtacgc
    22801 aagaacaagt cgaacaggcc caagaattat tgaatcttcc actaatggat aattggatcg
    22861 atagcagtga tccgagaaca cctgatttcc agttgacaaa aacagcaaaa gtcgcgaatg
    22921 tcatgataaa aatttaccaa gttctggcca ttcccgtgct gattgcttca atagcaggga
    22981 tattacttat gatactagga accgcatttg caaaagctga tcgtgcctat tatagttcct
    23041 ctttgattgt agtcaccgga ttaatactga cacaattggt ttttcttttc ggcgtctcgt
    23101 ggttttgttc ttatgcgcca gaaaaaaagg attatttttt aagtgtctac actggcgcag
    23161 gcgtgccatt aacgcaattc atcgtagtaa caagcatgct agttttcagt aaattgaatc
    23221 tgaaaaaagc tttatcttcc aaataaaaac atactaagat tagaggagaa catcattgac
    23281 gaatgatgtt ctcctctatt ttctattttt agattatagt gaagagtgaa agaaagttcg
    23341 aacaaagcct tgaggactat gagtccctat caaaaaccga acttctttac tgtcatttta
    23401 gaatcaggtt tacgtatgtt ggggccatga gcccgtgtat aggaaattgg aatcgaaatg
    23461 aagcagtaaa gttctttcaa aacttaaaaa ggaggaaaac aaatgcctta cattcttgca
    23521 ttagatgttt caatgggaca cagctacagt gtcctataca aagacgacac ctgcttattt
    23581 gaagacgaga tcgaacacac tcaaagagga tttcatgcat tactcgaaga gatccagcac
    23641 ttgcctgaac gtccattgat tgtttttgaa gcaacgggga tttattccag accagtcgaa
    23701 aagttctttc aagatcatca cttttcttac tgtttgttaa acccgttaga agcgaaaaaa
    23761 cagatggaag aaaacacctt atgcagttgg aaaacagata aatcggatgc ccatcggctc
    23821 gcacagactc atcttaaaaa tcaacgtcag ccaaaagaag tacaaagcaa tttttactta
    23881 gagatgcgcg atttagcccg tttttatcaa gaaattgaga agaaaatcac tcgtctgcgc
    23941 atggatttac acaactgcct tcagttgact tttccagagc ttgaacagtt ctttttaaac
    24001 aggctgaccc cttacgcatt gatattgatc cgtctttttc cccatccaga ttttgtattg
    24061 gcatctactc ggacaaaaat aaaaaataaa ctgatcaacg aaacacggaa aaaaatctcc
    24121 gcgaatcgtg ccgagcaaaa ggcagatcaa atcatccact atgcacagtg tgcctatcca
    24181 gctgttgaaa aagacagtat ccactgtcaa aaaacgatct attacgctga acttctccaa
    24241 gatttactcg aacaaaaaga agcactggcg acacaaatga tcaaaaaagc cgaaggctct
    24301 ccttgctttt tgttgtacca aacgttccct gggattggcg cactcacagc cgctttattg
    24361 ctcggtgaat taggagatat cacacgattt aaaacccata aacaattgaa tgcctttgtc
    24421 ggaatcgaca tccgacggta tcattcaggt aagtatactg gccaagaccg catcaataag
    24481 cgtggtaatc ccaaagcacg gaaaattatt ttcttcacca ttcgaaatat gattcgccag
    24541 caacgcgcag ctcctaatca tattgtcgat tattactaca aattaaaaaa gcaacctatc
    24601 cctaagaaag ataaggttgc cacagttgct tgtatgaaca agcttctgaa atgtatgcac
    24661 gctatggtta gggcacatac agagtacgac tatgcgtacg cggtctcagt ggaccattaa
    24721 ctaaaccact tacagtatac cacttctttg cccagatcga aaatttttta cgaacggggc
    24781 tttatttggt gtgccttgat tttgtcgctt tcgtttatga gctatttttc ttacaccttt
    24841 tacttgacta atcgtaggaa agggaactgt gacaaaagtc ttgtcgcagt tcctttttcc
    24901 gaataaacgg tgctcaaaag cttgcatctg cggtaataag tccaaccaag ttaaaaacat
    24961 aaaaagctgt ttttacttgg ctgttcttat tacttgatgc aaggcagctt tttcacaacc
    25021 tctgatacac ggtgttccag aagtaacttc ttcggaaata agccgaaatt tcacaaaaat
    25081 ttgaaaagca attttcttaa attccttctt atttcttgaa gctgcccact tctgacacaa
    25141 cctcctttgt gtaaaagaat cagtttctac tcgatttctt ctatcgtttc ctgcaaacgc
    25201 tgttcatatt ttttgtttcc tttatataaa tcaccaatcg tccatgccaa caggagaagg
    25261 acaagaacaa tgattacata agcgatttga tcagccaatg ctagttgagc tgaggtggct
    25321 tgctcgccga agaaactttc gatttgacct ggtaatcctc gtaaattcaa gtaagtcaga
    25381 ctaatcacag agaaccagcc aagtagtttt acaacaaatg aatttttgaa tcggctcccc
    25441 atctccgctt ggctattggt catcatcagt aaaggaatca tcgagaatgg caatgcaaaa
    25501 gccagaaaaa cttgggattc attcatcaag ttattcaaag cggtatgttc ttcgatcgtt
    25561 ccttttgtac ttgtaaacaa aacacaaatc aatacaggaa tcacagaaag caatcgtgtg
    25621 actagtcttc ttagccaaat tgggatacgc atatggatga agccttccat gatgacttga
    25681 ccggtcaacg taccagtaat agtagagttc tggccagaag caagaagtgc tacagcaaat
    25741 aaagtagaca agataccaga ttttgctaca gcaatcaaga taccattgct catagctgat
    25801 ggatcagata aagcttcata cagaccaaaa aaggaaggat cttggacaga accggttttg
    25861 aatacagcca cacccatgat caatagcagt gcattgacta gaaaagccat tgacaactgg
    25921 atattagaat cccaagtaga aaagcgtacc gcttgtgcta catcttcttc gtcttggtga
    25981 tcgatcttcc gagtttgaga aactgctgaa tgaagatata agttatgcgg catgactgtt
    26041 gcaccaataa tccctaaagc tccagtcagc ggagtttcgc cgccgatgac cggtgagtct
    26101 gagaatgtgt gactgttagg gataaatccg gcaaacaaac tgtgccaatc agggttagac
    26161 aagccaactt gatagacgaa aacgaataaa atgacgaaaa tcaagcagac aaccaaagct
    26221 tcgattttac gaaacccgac ttttgtcagc aataaaagga cgaaaacatc taacacagtg
    26281 ataaatacgg caattaccaa tggaatatca aaaagcaggt agagggcgat tgctgcacca
    26341 atgacttctg cgatgtctgt tgccataatg gctaattcag tcaagatcca taaaatgaca
    26401 cccaatgtct tacttgtccg tgctcggatt gcttgggcca aatccatttg ggtcacgatt
    26461 cctaattttg ctgccatata ttgtaaaagc atagcaatca gactagacat caaaataact
    26521 gacatcaata aatattggaa attttgtcca ccagtgatcg aagtggacca gttacctgga
    26581 tccatataac caactgctac taatgcacct ggtccagagt aagcaagaag tgttttaaaa
    26641 aaactttttc ccttgggtac atccactgtt ccatttactt cagctaaaga taaaccattt
    26701 gtatgttcaa taagtcgatg cttatgaaat tgtttgttgg attgattatt caagaaaaaa
    26761 ccacctttcc tatttatcta cgattacatt ctacacctca gaaagaaaaa attaaagaaa
    26821 aagatttggt atgcctaatt ttttttcgtg gcgtgtcttg ctggttaatg aaagaaattt
    26881 tgttcttctc taaaaaaacc actatttttt gcatgaaact aaagaatacg cctttctttt
    26941 ttcaataaac tttagtaaaa taaatcattg agagcaattc ggaaatagaa aggagagatt
    27001 tatatggtat caacaggtat tgtcgtactt attgctatta ttgctttgct aatcggcgca
    27061 gtaggtggct ttttcctagc gcgtaaatat atgaaagact atttagaaaa aaatcctccc
    27121 gttaatgagg acatgttgcg atcaatgatg atgtcaatgg ggcaaaaacc atctgagaag
    27181 aaaattcgcc agatgatgca gcaaatgaag aaccaaggga aaaaataggt tctgacccaa
    27241 agactttttg cgggctgatt ttttcagtcc gttttttatt tcgtaatttg ttagactatt
    27301 cagactattc agcaaaaaag acaagcacag acaatcctat gctcttgata aagacaaagg
    27361 aggagtaaaa tgtcgatttt tagaaaatta ggttggtttt ttcgacagga aaagaaacat
    27421 tatttaattg ggattttctc cctattcgca gtggccttgg tacagttagc tcctccaaaa
    27481 gtgattggga tcatcattga tgagatcgcc gataaaaatt tatcaataaa gatcatttcc
    27541 tggtggatct tattattgat ccttgcagct ctcttacaat atctttttcg ttatatctgg
    27601 cggacgaata tttggggaag tgcagctcgg ttggagaaag aacttcggca gcagctattc
    27661 aaacatttta cagaaatgga caatctcttt taccaaaaat atcggactgg agatttgatg
    27721 gctcatgcaa cgaatgattt gaatgcaatc caaaatgtag caggagctgg tatcttgacc
    27781 tttgcagatt cagtgatcac gggcggaaca acgatcattg cgatgatttt gtttattgat
    27841 tggcgcttaa cactgattgc gctgattcct ctgcctttac tagccgttac ttcacgagtg
    27901 ctggggtcaa aactccacga tgcttttcgt gattcgcaag aagctttttc tagtatcaat
    27961 gataagacac aagaaagtat tactgggatc aaagtcatta aaacatttgg acaagaaaaa
    28021 gaagatctcg cggattttga agaaaagatt gaagatgcaa ttattaaaaa caaacgagtc
    28081 aattttttag actccttatt tgatccattc attaccttga ttatcggtct gtcttatgtt
    28141 gcaacaatta ttttaggtgg aaagtatgtc ttggaaggca cgatctcaat cgggcagatg
    28201 gtctcattta ttagctatat cggtatgctt gtttggccga tgtttgcgat tggtcgattg
    28261 ttcaacgtat tggaaagagg aaatgcttct tatgatcgcg tcaatgaact tttgcatgaa
    28321 aagacgcata tcgtggaaca actgggtgca gtgcagactc cggcaaaagg cgagctgaat
    28381 gtagctattt cctcttttaa ctatccgaaa gaagaatcac cagcattaga acaaatcaaa
    28441 tttgcgatcc aagaaggaga aacattaggt attgttggga aaactggtgc gggaaaaacg
    28501 acaatcatca aattactgat gcgtgaatac gatgattatc aaggaaaaat tcaatttggc
    28561 ggatgttcga tcaaagaata ttcattgaat gctttattag gatcaatcgg ctatgtgcct
    28621 caagatcact tcttgttttc gatgaccatt cgagataata tccgtttttc taatccaaaa
    28681 ctttcagaag aagaagtaga gcatgcggct tctttagcat ttatcaacaa tgaaatcaag
    28741 gaattaccaa aaggttatga taccttagtt ggagaacgtg gagtctcttt atctggcgga
    28801 cagaaacaaa ggatttcgat tgctcgagca ttgatcaaaa atcctgaatt gctgatctta
    28861 gatgatgcac tttctgcagt agatgccaaa acagaagaag ctatcttgca taatttaaaa
    28921 gaaataagaa aagagaaaac aaccatcatt gcagctcacc gtttaagcag cgtgatgcat
    28981 gcaaaagaaa tcattgttgt cgatcaaggg aaaatcattg aacgaggaac ccatcatgag
    29041 ctgcttgcat taggcggctg gtatgaacga atgtgggaga aacagaaatt agaagcgaag
    29101 atagaaggag gcgaggcctg atggatgaaa attatcaatc ggaatggaca aaatcgattc
    29161 cacttaaaga acagaagaag atcatcaaac gactgatgaa gttcgccaaa ccttttagaa
    29221 gaacgttcat cgttgctatc ttatttgcgt ttgccctgtc tgtgatcaat gtcctattgc
    29281 caaggatcat ccaaactttt atggatgatc atctggctaa acagtcggct acgacacaag
    29341 ttattttgtt ttttgctggt atgtacttgt ttggagtcat agtcaaaagt atcatttggt
    29401 ttttccaatg gtatttgtat tccatggctt cattgaagac ctatcagtat gttcgggtaa
    29461 gattatttga aaagctgcat acactaggta tgcgttattt tgatcagaca ccggcaggat
    29521 cgactgtttc tcgagtgaca aatgacacag aaacgctatt tgaattttgg tatgtctttt
    29581 tgatggtatt gactgggatc tttgcagtcg tttcttcctt cattgccatg tttcaaatca
    29641 atggagaaat tgccttgtgg actttggtgt tcttgccgat tttaggaatc gtcatctggt
    29701 attaccagaa attcagttct cgtatttacc ggaatatgcg ggaaaaatta agtcagctga
    29761 acacgaaatt gaatgaatcg atttccggca tgcgtattat ccagcagttt cgccaagaag
    29821 ctcgtttagc taaagaattt gaacaagtga acaacgagta tttagacacg cgctatgcga
    29881 tgatcaaaac aaattcgatt ctgttaagtc cgatcatcaa ttttctatat gcattggcga
    29941 ttgcgttggc gttgactttg tttgggatcg atgcgctgca ttctcccgta gaagttggac
    30001 tgatctatgc atttacgacc tatgtccagg gattttttaa tccaatgtct caaatgatgg
    30061 actttttgag cacctttaca gatggaatgg ttgccggtag tcgtattttg aaaatcatgg
    30121 atacagaaga atatgcaccg caacaaaatc ctggagcaga tcaagcgatt acttcaggta
    30181 agatcgagtt tcgcaatgta agcttttctt atgatggaga gcacaatgta ttgaacaata
    30241 tctcttttgt cgctaatccg ggagagaccg ttgcattagt ggggcacacc ggaagcggaa
    30301 aaagctcgat catcaacgta ttgatgcggt tttacgaatt ttatgaaggc cagatcttga
    30361 ttgatgatca tgacattcga gaatatccaa tggaagagtt acgtaagaaa atgggattgg
    30421 tcttgcaaga tgccttcttg ttttatggag atatcgcagg aaatatccgg atgatgaatc
    30481 caactatcac agacgaacag attcgccatg cagctgagtt tgttcaagca aacaagttca
    30541 tcgatgaatt accaaaagga tatcactcaa aagtgatcga acacggtgcc agctattcta
    30601 gcggacaacg acagctgatc acttttgcgc gaacaatcgt gacagatcct aaaatactag
    30661 tattggatga agcgacagca aatatcgata ctgaaacaga aggattgatc caagaaggat
    30721 tgacaaaaat gcgacaagga cggacaacga ttgcgattgc ccatcggctg tccacgattc
    30781 gggatgcaga attgatttta gttttagaca aaggtcatat agtagaacgt gggaatcatg
    30841 aaacacttct agcgcaaaat ggtttgtatg cagatatgta caaactgcaa agcagtggtg
    30901 tgtaaaaagt ggttgtgaca gggctccgtt cagctttagg tgataagaaa aaaattgtaa
    30961 aaaaggcttt ccagattttt gcataaattt ggcttatcac cgataagctg gtactggaac
    31021 accgtatatt agaagttggg acagtagcat agtagcattt tgacctaagc gctagagtga
    31081 aaataaaaca gagcttccca gattcttcat ctgtgagcgt tagtgtcgta attaccagat
    31141 ttaaaaaaaa tcacgacagc tagtcagaag tgcccaatag ccgctaagaa agcgttgaca
    31201 aaagtgcttg ctttcgataa tataaagaaa acgatacaac ttggatagtg attgcctgat
    31261 gcaagctaaa cctgatgagt ctcccatggt tccatggacg gctttttggg tttttctttt
    31321 tgttttggat acagccctat atatcatgga aaggagtaaa acatgctgat tgagaaagta
    31381 ctgaataaca acgtcgtaat aaccagggat gaaagaaatc aggaaatgat cgtgatggga
    31441 aaaggtttgg cattcaaaaa gaaagtgggt gacccgattt ttgactcatg catcgacaaa
    31501 aaatttcagt tgacaaatga cggactttca caacagttcc aagaattggc acaagcgatt
    31561 cctcttgagt acttagaaat ttcttgtgaa atcattgaat atacaaaaga ggctctacaa
    31621 gtccagttga atgattcggt gtatattgca ttgacggatc atttgtatac ggctatcgaa
    31681 cgtcacaaaa tgggaatatt tgtaccaaat gtattgctgt atgatatcaa acaactattt
    31741 cctaaagagt atgccatcgg gaaaaaaaca gtgaagaaaa tctcggataa ataccacatc
    31801 aagttatcag aagatgaagc cggatttatc gcattgcatt tagtcaatgc acaaggcatt
    31861 caaagccaac aagacaatac ctacctgttt actacaacaa tacaggatat tctaaatatc
    31921 gtccgctatt actataagat tacatttgat gaagattcag tccatttttt tcgattcgtc
    31981 acacacctac gtttttttct gacacgcgtc ctagatggag aaaaatcaga agtgggagaa
    32041 gtttctgagg aattgtacca ggttattcaa aaaaaatata aggatacgtt tggttgcgtt
    32101 gagaaaatag cagacttttt agccgagata ttcaactacc agatgtccac ggacgaaaaa
    32161 atgtatttaa cgatccatat tgcgcgttta gtgcatgaga atcgttgtaa ataaataaaa
    32221 aaaggaggat aaattaacaa gaacgcatga aataagggta gtgacattca gttggctaaa
    32281 ccttccacta ataaattgga gggaatagaa atgggaaaat atcaagaatt agcgagcaaa
    32341 attgtagaaa atgtcggcgg gaaggaaaat atcaatggtt tgacacattg tatcacaagg
    32401 cttcgtttca aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa aaatatggac
    32461 ggtgttgtga cggtaatgcg agcgggagga cagtaccaag ttgtcatcgg gaatcatgtg
    32521 ccagtcgtgt ttgaagaggt tataaaagca ggaaacctta cttttgatga ggctgtttca
    32581 acagaaaaaa tgagaccgtt tgatatgctt attgatggaa tatcgggctg tttccagcca
    32641 ttccttgcta ttctagctgc tggtgggatg attcgtggac tgacagcatt acttgtcttc
    32701 ttaggcgcat ttgaccgcgg ttctggcaca tttgttatgt ttgataatat cggtgattct
    32761 gtattccaat ttatgcctgt tattatcggt ttaacagctg cccgtaaatt taaagtaaat
    32821 gaattcgtag ggatgttgct tggggcagca ttaatgaatc ctagcttgtc gctggaagct
    32881 ttgagtggtg cagcagaagc accactgact accatatttt ctggcactat ttttgaagca
    32941 cctatttatc aaacagtatt cggcattccc tggattgcgc gtaactacgc ttcttctgtc
    33001 attcccatta tctttattgt gttgttagct agccaaattc agaaaccaat taaaaagtta
    33061 gtacctgaga tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat tacaatgccc
    33121 ttaggttttt tactcgttgg accagcattc acattcgcaa ctgatatttt aatggctgga
    33181 tttgaaacac ttttagcact ttcgccagtt atttatggag caatcgtagg cttcttctgg
    33241 caaattctag tgatgtttgg tctccattgg gcaatcgtac caatgggatt gatgcaattt
    33301 tctgtgaatg gttggcaaaa tatcatgaca cctgttgcag tagtgtcttt tggacaaact
    33361 gcagcactta ctgcactcta ctttaagctt agaaacccaa aagacaaagc aatcgcaata
    33421 ccagccattg tttctggtat tgtggggatt acagagccag ctatttatgg gtttacattg
    33481 ccacgtaaaa aaatatttat ttttacctgt gtcggtggtg cgttaggggg cgcgtatagt
    33541 ggattaatga atttaacttc ttggaatcca ggtggattag gcatctttac aattcccaac
    33601 tatattagac cagatggtga tttaacggat gtgattaatg tattgattgg aatcgccatt
    33661 gcgatggtgg tatcctttgc gctgactttt ttcttttgga aggaggaagc aggtgaaaca
    33721 gatgatagac agaaaagatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc
    33781 gctccactga atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc
    33841 ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg
    33901 ttcccgacga aacatgcaat cggaatggtt tctgaagcag gtctggaatt gctgattcat
    33961 gtcggattgg atactgttca gttggaaggg aaatattttg aaagcttggt gcagcaagga
    34021 gaaagagtga aacaaggcga tctgttgctc cgcttcgaca aaaagaaaat cgaagaggaa
    34081 ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg
    34141 gaaaatcaag gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg
    34201 gttaggtgaa agaatggact aggcttaatt attcttttta gtccacttct ttattttcaa
    34261 aattgaaagg ctctttcatg atggctgagc tgatagggcg tttatcaaat ttttgtccta
    34321 cagaaattag atggactaaa ttctgttgat aattttctgc aaaaacacaa ctatataaca
    34381 cgtctagaag ataacaaatc gatgtattga tcgtaaaatt accaattttc gaatacagtc
    34441 gttcgcgtgt cgtaagtcgc aaagcggctg tactcattga agatagcgtt gaatcaccga
    34501 tactggtcaa agctaaaatt ggaacatgat tttgtttcaa tatttctgcg gtacgaagga
    34561 tcacattgtt ttcacccgta taagaaatca gaatcccgca catatcactc gtgcaattgt
    34621 acgcttcata gaacgattca ccaaaagtgg tgacaatcgt gacatccatt ttgatgcgct
    34681 tcattttcag catgaaatct tgagaaatca aagtattcga gttactggcg aaaatcacga
    34741 ttttttttgc ttgaagcatt agttgtttgg ctttttgcag ttggtcatga tgaatgagag
    34801 aaagtgtatc ttgaatcgtc atctgttcta aagaagccat tttatttgcg atcgtcataa
    34861 taccgtctgt ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc ccttcaagat
    34921 aggctgtttc agctaaaaaa gcattacgaa aactgaccca gccatcaaaa cccatttttt
    34981 ttgctacacg gatgagtgtg gacgggtgga cataacatcg ctcagcaatc tcttggatgg
    35041 tcatttcgcc aagtgctgga gaatcgctta agagaaactg aatcacttca ttttctgaag
    35101 gagaaaagga gatttctttg attttttcac gtaataacat gtagatacct tcttcttgtt
    35161 tttttacgta aattataact tttttgaaaa tgatttgtaa acggatttat gttatatagc
    35221 ttaataaaga cattttttga caagatattt acaaaaatcc agtagaaaac actaactttt
    35281 gtaaatgtta ctggaaagaa acgaatttct attgtttgtt ttgtttacaa aaaagataat
    35341 aaaagtgtaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg
    35401 gtggaggcag cagttacacc cccgagttga tggaaggatt tatcaaaaga tatgaggaat
    35461 taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg
    35521 tcagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaaa atctatgcaa
    35581 ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg
    35641 gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc
    35701 aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca gtggttttgg
    35761 caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc
    35821 caagcgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgataggat
    35881 tatgcaatgt gccagtaatg gcgatgatga cagaaccaac aatgcttggc aagaaaaaag
    35941 aagagctgat ctacaagttt gccggtctaa atcacttcca ttggcacaaa gtagcagata
    36001 ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa gacaatggat
    36061 tgccgaaaaa tatctttgat atcccgtttt tcaaagaaca attaaaacaa atgaacatga
    36121 tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgctggaa catgctttag
    36181 aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa gcagagctgt
    36241 ttgaattata caaagatcca gaattggatc ataaacctga acaattgcaa aaacgaggtg
    36301 gtgcttatta ttcagatgct gcctgtgaaa cgattgcttc gatctatgga aataaagaaa
    36361 cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgatctgccg gcagattgtg
    36421 tagtagaagt cactgcctat gttggcgcac aaggcgcacg aaatgtagcg tttggttccc
    36481 ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaattg ctgacgatcg
    36541 aagcagctgt gactggtgat tatggaacag ctttacaagc gtttacgatc aatccgctta
    36601 tacgatcagg aaagacagca aaacggatca tgaatgagtt gtttattgca catcagcagt
    36661 atttaccgca attcaaggaa acaatcagcc ggttaaaagc agaaggaatc gaagtccaag
    36721 atgaactggc aagagaatta tcaagcaata cacatgtaat ggaggaagta tgatgggatt
    36781 cagaaaagat tttttatggg gcggagctac agcagccaat caatgcgagg gcggttatga
    36841 tgaaggcgga cgtgggttag cgaacgtaga tgttgtgcca atcggagaag accgttttcc
    36901 tgtcattact ggaaaaaaga aaatgtttga atttgatgaa aatcattttt atccagcaaa
    36961 aattgcgatc gatatgtatc atcattataa ggaagatatt gctttatttg cagaaatggg
    37021 gttcaagact tatcggatgt ccattgcttg gagtcgtatt tttccaatgg gcgatgaaaa
    37081 agagccaaat gaatctggtt tgaaatttta tgaggatgtc tttaaagaat gtcaaaagta
    37141 cgggatcgag ccgcttgtga cgattacaca ttttgattgt ccgatgcatt tgatcaaaga
    37201 atatggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt gccgagtcat
    37261 cttcaatcgc tataaaggac ttgtcaaata ttggttgaca ttcaatgaga tcaatatgat
    37321 ccttcatgca ccattcatgg gcgcagggct gtattttgaa gaaggagaaa atgaagaaca
    37381 aatcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc
    37441 tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc
    37501 atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt
    37561 cttcattgat gtccagtcac gaggagaata tcctgcttat gcattgaaag aaatggaacg
    37621 aaatggcatt cagatcaaaa tggaacctgg ggatgccgca ttgctgaaag aacatactgt
    37681 tgattttatc tccttttctt attattcttc tcgagtttcg actactgaca acgaactctt
    37741 ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgg aagcaagtga
    37801 atggggttgg caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg
    37861 ttatcaaaaa ccactattta ttgtagaaaa cggcttaggt gcagtcgata cgcccgatga
    37921 gaatggttat gtggaagatg actatcgaat cgactatctg gcagcacata tccaggcaat
    37981 gaaagatgca gtagaattag atggagtcga tctattaggc tatacgactt ggggatgtat
    38041 tgatttagta tcagctggta caggtgagat gaagaaaagg tacggattca tttatgtcga
    38101 tcgtgacaac gaaggaaatg gcacattagc ccgttcgaag aagaaatcat tcaactggta
    38161 taaaaaagta attgattcaa atggagaaaa tctttcgaat gattgatcaa tgatttataa
    38221 aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc
    38281 agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga
    38341 gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc
    38401 tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggcg cccctggcgg
    38461 ccaaatgaac aatacggcag aaatcgaaaa ctatcctggt ttcgattcaa taatgggtcc
    38521 tgatctagct tataagatgt atgaaggtgt ttcaagattt gggacagaaa atgcttatgg
    38581 aatcgtgcaa gatatcaagg atcacggcag ccacaaagaa gttatttgtg aagataaaac
    38641 ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat taggcgtacc
    38701 tggcgaggaa gaatttgctg gacgtggcgt ttcttactgt gcagtttgtg atggtgcgtt
    38761 tttccgtaac aaacgtctag tagtgatcgg aggaggagac tcagcagtag aagaagccat
    38821 ctatttgaca caatttgctt ctgaagtagt catcgtccac cgtagagatg aattacgtgc
    38881 acaaaagatc atccaagacc gtgcatttgc gaatgaaaaa atttccttta tgtgggatac
    38941 agtcgttgac gaaatcgttg ggaatgaaat ggtagtgaca ggtgtgaaag cacgtaatgt
    39001 caaaaccggc aatgagtttg atttggcagc agacggagta ttcatctatg ttggactgga
    39061 tccattaact gcaccattta gagaagcagg gttgacgaac gaagctggct ggattccaac
    39121 agacaacgaa atgaagacga atataccagg cgtttttgcc atcggagatg ttcgtgaaaa
    39181 agatttgcga caaatcacta ctgctgttgg tgatggtggg atcgctggac aacaagtgta
    39241 taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt ggaagaacgg
    39301 tttaattgtt cttttactct tttttttcgc gtgaaatcta ctgaatccga tttatgcgaa
    39361 tacttgcaac cgtgcttgat aaaagaatcc tttcttgatt taagcgtttt ttggtcctca
    39421 tactttcata tctaataagt gaatgctata cttattaaga accatatttc agaaagaggt
    39481 gttattttca tgtcttggga acaagtgtat gaacaatggg tacatgaaga aaatttagaa
    39541 gaaaacttaa aaaaacaatt aaatgatctg ggtgaagatc ctgaaaaact tgaagatgct
    39601 ttttatgcac cgttggaatt cggtactgca ggcatgcggg gaatcttagg gcctgggatc
    39661 aatcgaatga acatctatac ggtaagacaa gcaacagaag gacttgcacg tttcatggat
    39721 acacaaggcg aagaaacaaa acgccgaggt gtcgctatcg cttatgactc acgccatcaa
    39781 tcaccagaat ttgccatgga agcagccaaa acattagcac atcacaatat cccatcttat
    39841 gtattcgaaa gtctacgtcc aacaccagag ctttcttttg cagtccgtca tttgaagacg
    39901 tttgcgggaa tcatggtaac cgcatcccac aatccagcag cttacaacgg atataaagtc
    39961 tacggagaag atgggggaca aatgcctcct gcagacgcag atgcattgac gaaatatgtc
    40021 cgtgaagtca gcaatccgtt aaaagttgaa gtattatctg acgaagaagc tgaacatagc
    40081 ggattgatca cgatcatcgg agaagaagtg gatgccgctt atctagaaga aatcaaagca
    40141 gtaacgattg atcatgaatt ggtggaaaca atggggaaag atttgaaact tgtgtacaca
    40201 ccacttcacg gaacaggaaa aatgttgggc gaacgtgcgt tgaaacaagc tggattcgaa
    40261 caattcgtac tagttccaga acaagctgtc gcagacccag atttcactac agtcaaatca
    40321 ccaaatccag aagaacactc tgcttttgag tatgcgatac gacttggcga gaaagaaggc
    40381 gctgatttgc tgattgcaac agatcctgat gcagaccgtt taggtgcagc cgttcgtttg
    40441 ccaaatggct cctatgaagt attgaccgga aaccaaatcg gagcactgtt tgctcaatat
    40501 attttggaag cacaccaatc gcgaggcact ttacctgaaa acgcagcggt attgaaatcg
    40561 atcgtttcta gcgaattacc tacagcaatt gcggaacact atggggctac catgttcaat
    40621 gtattgacag gattcaaatt cattgctgaa aaaattcaac aatttgaaga agatcattcc
    40681 catactttta tgttcggatt cgaagaaagt tacggttatt tagttaaacc tttcgttcgc
    40741 gacaaagacg cgatccaagc attgctctta ttagcagaag tagcagctta ctataaaaaa
    40801 caaggcaaaa cgttgtatga tggcttgcaa acgatttttg aaacctatgg ctactacgca
    40861 gaaaaaacga tttctgtgac aatgagcgga caagaaggtt ctgcgaaaat ctctgctttg
    40921 atggaaaaat tccgtgaaga agcaccaaca gaatttgccg gagcaaaagt catccaaaca
    40981 gaagacttca aagagttgac gagaacagac cgtgaaggaa acgtagaaaa actaacgaca
    41041 ccgccatctg acgtattgaa gtatacgctg gaagaaaatg gctggatcgc tatccgtcct
    41101 tcaggaactg agccgaaaat caaattttat atcggtgtaa aaggaaactc ttcagaagac
    41161 gcagctgaaa aagtagccgc ttatgaaacg attatccaaa agatgacaga agaataaaaa
    41221 aacttgagac tgatggaaaa agtggtaaac tttagaagtc agtctttttt caagttggag
    41281 gtgaatggat tgccagacac gcaaaaaaca aaagtagaga aagtcagcca actattcaaa
    41341 atgttgagtg accccactcg tctcaaaatt ctgctgtatc tgaaagacgg ggaacaaaat
    41401 gtgacagcaa tcatccaagc agtggaaatg gaacaatctg ctgtatcgca tcaactgcgt
    41461 ttattaagag aaaaccatgt cgtcaagtcc catcgtgagg gaaaagcgat tttgtatagt
    41521 cttgatgatt ctcacgtact ggatattttg aaccagacac tcaaacatgt ggaacatcaa
    41581 taacgatagg tgcaaataga gacaaactgg actgtgtcaa cgaatcttct tcgctgacac
    41641 agtccagttt ttttaaagaa aaattgttag tctgtttctt cggtggtttc agttccataa
    41701 taactatggt cttctaggtc taaagcattc aaaataagcg aagctgtttc ttcaatagat
    41761 aaagaagcga cattgattac tacgcagccc aatttttcat aaagatcatt cgcaaaatcg
    41821 agttcagctc gtattttttc gatatcggaa taagcagtat caggatttaa tccgtaagag
    41881 cgcatccgtt ctttacggat attgtttaaa atatctgggt tattcgtcag tcccacgatt
    41941 tttttaggat cgatctccca aagctgtttg ggaatatgtg cttgtgggat caaaggtaga
    42001 ttggcaactt ttaaattttt gttagctaaa aacaagctca aaggcgtttt ggaagttcgg
    42061 gatacgccta gcaaaacgac atctgcttct agaaacccac gaggatcttt cccgtcatcg
    42121 tattttacag caaattccat tgctttgatc cgtttgaaat agttctcatt caataaatga
    42181 agggcgccag gttgccgaat gggcttgata ccagttaagc gctcgatttc tgatatcggc
    42241 ggagtcatca catcaaaact aaataattga tgttcttcac aaaaagaacg cgtcatctcg
    42301 atcaattctt gattgatcaa tgtgtgcagg acaagagctt tttcttctag tgcatcttgc
    42361 agtgcctgca tcaatgtttt ctcatctgtc acaaatgttc tgcggaataa attgaactca
    42421 acgctaggat attgggccat agtggcttgc gctagttttg tcgcagtttc tccagcagag
    42481 tcggagatga caaaaaaagt aacgatttct gtagaatcgg aagacatgga taaaacctca
    42541 cttgcatttt ttttatgttt attataccat actctagaat gtgtacttcg taatcatttt
    42601 gatttggagg aataagtcat gaaaaaacag acgttgatcg aacaatcact agaaacattg
    42661 aaaaataacg gttttaaata tactaaaaaa agagaagcat tgttaactta tttagtgaag
    42721 cgcaatcgct acgtatccgc taaagaagtt tttgactata tgaatcagga attcaaagga
    42781 gtaagttacg atacgattta tcgaaatctc catgattttg cagaaatcga tttactggaa
    42841 gaaaccgatt taaatggaga gatgaagttt cgttttcatt gctgtcaagg ggaaatcggc
    42901 catcaccatc actttatctg tacggtgtgc ggaacaacga aagaaatcca tatgtgtcca
    42961 atgaattatt tcgaagaaca gctcggtgga tgtacgatcg aagggcatag attcgagatt
    43021 ttcggaagat gtgaaaattg tcaataagga tttaaataaa ataacatttg ataattttaa
    43081 aaaaaagcaa gatttccttg acggtcaaaa cagcattaga tataatattt acaggacagt
    43141 cttgtttttt gagatatatt tcaataacaa ttacggttac tttttaagct cggagggagg
    43201 gaattaacat gtcaaaaaca gtcgttcgca aaaacgaatc acttgacgat gctcttcgtc
    43261 gcttcaaacg ttccgtgtca aaagcgggta ctttacaaga atctcgtaaa cgtgaattct
    43321 acgaaaaacc aagtgtaaga cgcaagaaaa aatctgaagc agctagaaaa cgcaaaaaat
    43381 tctagttctg tgatgaattg gagttggtag tgtgtcacta ctaagtagat taaacgatga
    43441 catgaaaaca gcgatgaaag cgaaagataa ggaatcttta caagtcatcc gaatgatcaa
    43501 gtcatctata caaaacgagc agattaaaga aggtcacgac ttgacagaag aagaagaatt
    43561 aacggtgttg tctcgcgaaa tgaaacagcg acgtgattct ttgcatgagt ttgaagaagc
    43621 aggacgtgac gatttagctg aaaaagtaaa aagtgaaatt gtgatagtcg aaaaatacat
    43681 gccggaacaa ctttccgacg aagaaattcg acaacttgtt caagaagcaa tcacgcaaac
    43741 tggtgcttca tcaatgaaag aatttggcaa agtcatgggt gcgatcatgc ctaaagtcaa
    43801 aggaaaagca gatggaaacc aagtaaatgc cattgtgaaa gaactattac aatcttaatt
    43861 ttatcattta aactgcggac aatcattgtc cgcagttttt ttgtgctcgt aaaaaagaga
    43921 tctaaaaaat agctagacga aagaatagga ttctttcatc tagctgtttt tttcctgttt
    43981 aatatttctt atgttaaatt gtgtttgatc tggttattct tctggtagtt catttgcttc
    44041 ttgtttatac cctgataggc gataggcgta atagaagagt aagctgccag catacccaag
    44101 taacgttggg accatccagc caagtcccaa tgcagcaaaa ggaacgtgat gatgataaaa
    44161 ttcgagcatg cttgctacaa ctctttgttg actcactaaa gcaggcgaat ttgccagcat
    44221 atccaatacg gcgggaacag cagcaaacaa catcgttact tgatagacaa atggtgtctt
    44281 ttggaaaaat tttgctgtta aggaaagcaa aatcaacgca agcgacaatg gatacaaaag
    44341 catcaatact ggtaaagacc attggatgat attgtccaaa ccagcatttg caacgacaaa
    44401 agagacgaaa gtcgttaacc gcagccatgt catataactg actttaggga acagtttatg
    44461 gaaatcttgt gcaaaagaaa caacgagtcc catagcggta gtgaagacac ctaatgtgac
    44521 catcactcct aagaagctag cgccgaagtt acctaaataa aaattagtga tctgagaaag
    44581 tgcagttccg ccattttctg ataaagacat cttattcaaa ctcatcgcac cgagaagaac
    44641 aagaccaaaa tagatcatga cttctagcgc aacgctcagg ctaccagctt ttgctgttaa
    44701 ttctgtgact tttttatctt tgaaaccaag gtcacggact gcatggacaa aagtgacact
    44761 taaggcaagc aaggctactg catcaaccgt gttataacct tctaggacac catttgttaa
    44821 agcgtttgtt tggtaagtat ctgcagcaga ttgatttaga tttcccattg ggtggaataa
    44881 agaaacgaca aaaatgattg ctaataatgc aagaaacaag gagtttagat actttccgac
    44941 gtatttgacc aaccgtgtgg gatggacagt aaggaaataa gcaagtaaaa agaaaagagc
    45001 agagaataaa aacataccta atgaattata tttttcaggc aaaaatggtt tagcagccat
    45061 ttcaaaagca gtagcagctg tccgtggtgt accgaaaaat ggtccgatcg tcaaatgaac
    45121 aaggattaag aacaaggcag cataccattt tccgacaggt ttagctaagt catacagacc
    45181 atcacttttt gtcactacta cagctaagat agctagtaat gggaaaagag cgcctgagat
    45241 ggcaaatcct acgccagcac tgacccagtt tgcgccagcc atttgaccta agtggacagg
    45301 aaagattaga ttacctgacc cgaaaaacat cccgaagata agtgaactga ccatcaatag
    45361 ttgtttccat gaataagttt ttgtttcttc cataaatttt tcctccaata attatcattt
    45421 tgattgacga tataaacgtt gcagcaatta gtaatgccac taatatcaga ttccatgaaa
    45481 accaccacct taaaatataa aaaaaagccc ttttccactt gggaaaaggg ctctagattc
    45541 tagaacggta ccaccttttt tcgcttaaaa aagcttcttc attcacggac gctttttgat
    45601 aaagttatcc gctagcctga taatggaggc agccacaatg atctacgttt tcgacattga
    45661 ctcggaaatg atgttcgtct attcgattta ttgtcatttc tcacttacat gactcactgg
    45721 gaataaatcg ttgaatagcc tactgttttc ctcgacgctt tgctgtatgt ttatttgttg
    45781 acgtaataat acacctgttt tttttgaaat gcaagaatat tttaactatt ttgttttctt
    45841 tttttgaaac aagaaaaata cctagagaat acctctgaat gaaacggaat attttgttct
    45901 ccactttcat ataacttaca ttctcagatt ctttttatca aaacgagacc tttagcgaat
    45961 ggaaacagct attttttaac gtttcttggt acgtatggtt aaacgactct tttacaacct
    46021 tttcttattt gctatatagt ttcttctgag tatggtaaaa taggaaagaa tgaatcgaaa
    46081 aaaaggggat gtctattttt gacggaagag tctagttctt tagagattag attgacagct
    46141 gctgatgatg tcagcatgct tttaggtgca cacgacaagc atataaaact gatcgaggaa
    46201 acaacggaaa caaccatcca tacacgtgga gaaatcattc agatcattgg tgaacaatcc
    46261 cagatttcct tggctgcctc agtcattcgg acattacagg aattgatcaa acgaggaatc
    46321 cacgtaagta caccagatgt cgtgacggca ttgaaaatgg ggcaaaaagg tacattagat
    46381 tattttgttg agatgtatga ggaagagatc gtcaaagacc gtaatggaaa acctatacgg
    46441 gtaaaaaaca gcggtcaaaa gaaatacgtt gaatcggtag ccaaacatga catcgtattt
    46501 ggtgtagggc cagccggaac agggaaaacg tttcttgccg tagtattagc aattgctgcc
    46561 ctaaaaaaag gcgaagtgca aaaaatcatt ttgacgcggc ccgctgtaga ggcaggggaa
    46621 aacctaggct ttctacccgg ggatctgaaa gaaaaagtgg atccttatct gcggcctgtt
    46681 tatgatgctt tataccaaat ttttggattg gatcatacga accgattgat ggaacgaggc
    46741 gtaatcgaaa tcgcaccatt agcttacatg cgggggcgga cattggatga tgcattcgtt
    46801 atcttggacg aagcacaaaa cacgacagtc gctcagatga aaatgttctt gacccgtctc
    46861 ggttatcaat caaaaatgat tgtcaatggg gatacaagtc agatagactt gcctcgaggg
    46921 acgacaagtg gtcttgttca tgcagaacga acactaaagc aaatcaaaaa aatcgatttt
    46981 gttaattttg aagcaagtga cgttgttcgg caccctgtag ttgccgaaat catcaaagct
    47041 tatgaaaagg cggatcttca tcaagaataa gctttgctta ggaggataag cacatgttga
    47101 ataaaacact gcagaaacta caagaaaaac tcggaaaggc agcattgcct ttccttctgt
    47161 tcttgttttt tatcattgtc tgcggcgtta catttagtag tgtcaaacaa aaggccaatg
    47221 atttcagaga aggccaagta gcagaagaga gtatccgtgc gaataaaacg atcgagaaca
    47281 cggaagaaac cgaacaaaga agaaaactgg cagcagaagc agtgactcca gaatatactt
    47341 atcaaaaaga tctggcagat gaccagaaca atcggatcaa gcaattattc gaattgatcg
    47401 ataagacgaa tgataccatc aacaaatctt ataatgacaa ggtagataag gcgaaggaag
    47461 atgaaaatat cccgaaacct actacagaag agcggattgc tacactgaag aaaaattttg
    47521 aaaacgtgaa tgcagaaaac gttgcgtttt accaaaaact gccgaacaat ttttattcga
    47581 cgatatttga aatgactgaa acggaaattc acacggttag ggatgaaagt ttgaagctca
    47641 ttgacgaaca gatgagtaaa caagttcgtg aaagcgaatt ggaagctttt aaacaagaag
    47701 cagaagatca gattcagtat ttgaatgtca caacagagca gcaacaaatg attcgctact
    47761 tagtggatca aggcattgta gtcaatgatg tattgaatga gaaaaagaca gaagaattaa
    47821 aacaatctgc acgagaagct gtacaaccag tgatgatctt ccaaggagaa atcattgttc
    47881 gcgaaggaaa tcaaattgat gcttcagcga tgaaaaaatt ggaattacta ggattgacga
    47941 atcaaacgac ttcgattttc ccactcattg ccatggtttt agctgttttg cttcaaatcg
    48001 gcgtactgat ttataattca cttcaattcc aaaatgcagg agaaagaaca aaatacgtgc
    48061 ttttctatgt gacagctatg tcagtcagca tagtgctgat gaaatttttc caattattcc
    48121 aaacggaaca agctacttat atcccgctgt tttatcccgc tgcttttgca ccacttgttt
    48181 taagcttttt cttgaaccga agagcaggga tcatggctgg tattttccaa gcagtatctg
    48241 cacttttcat tttttatgat tcgattggca cgaactttct aacgattatc ttgatgtctt
    48301 atgttttctc tgggctcatg gcaacgattg tcagacgcaa acggatctca gaacaaggat
    48361 tatctgctgc catgtgggtc attatttttc ctgtgatgat ggatgtcatt ttgatcgtct
    48421 atcaaggaat gagctttggt gatgcaacga cttggttaat gcttgtctgc gggttcactg
    48481 gtgcactctt ctcattcttg ctgacgatcg gtctacaccc atatattgaa ttgatggtca
    48541 atgatgacag tgtaattgta ttaaatgaac tcagcaatcc aaatcatcct ttattgaaac
    48601 agttgctgga agaagcgcca ggtacttatc atcatagtat gatggtagct aatctaagtg
    48661 ctaacgctgt agcagaaatc ggcggacgtt ctttgctaac tcgagtagct tgctattatc
    48721 atgatatcgg caagatcaaa catgctagct ttttcgttga gaacttgcct tcaggagcgg
    48781 aaaacccgca caatttctta ttgccagaag acagtaaaca tattatcttt ggacatgtga
    48841 cagatggagc caaaatattg gaagattata atatgccgca aatggtgatc gatatttgca
    48901 aacagcatca tggaaccacg ctaatgcgtt atttctacat caaagcgaaa gaacgcaatc
    48961 cggaagtaac agaagagcaa ttccgttatc ctggaccaaa accgcaaagt cgtgaagcag
    49021 ggatcgtcaa tattgctgac agttgtgagg ctgcagtacg ggcaatggat catccgacga
    49081 gcgaaaaaat cgaaagtttt gtccataatc ttatcgaaga gcgtatttcc gatggtcaat
    49141 tagacgatag tggactgacc ttgaaagaaa tccgaaaagt ggaaaaatca ttgatcagcg
    49201 gtttaagtag caccttccat tctcggatca aatatcctaa aatgaaaaca gaagttgaga
    49261 agataaagga agaacaagaa gaatagaaag aagcgatgta taatggatat tacctttatg
    49321 gatgaaacaa accaagtctc agaagagaaa atgaaagaaa tcgatgactt gctgcaattt
    49381 gcagcatctt atttaaagct gccagaggaa acagaaatgt ctgttacttt catggacaat
    49441 gcggcaatcc aagtaatcaa tcgtgattac cgtggaaaag atgcaccgac agacgtcatc
    49501 agttttgctc tggaagaaga aggcgaagac gaaatcccag tgatttttga agatggagag
    49561 aatcctttgc ctcgtgtact aggagacatc atgatttcta ttgaacgtgc tgctgaacaa
    49621 gcagaagctt acggccattc atatgatcgg gaacttggct ttttagcggt acatggattc
    49681 ttgcatatca acgggtatga ccatatgaca ccagaagatg agaaagaaat gtttggttta
    49741 cagaaagaga tcttggatgc ctatggactc aaaagataaa aacgtagaga aaaacaaaca
    49801 tttcctgatg tctgtggaat ttgctgtcca aggagtcaaa accgtttttg aggaagaacg
    49861 caatatgagg aaacatgttg cgttcggtat ccttgcttta tttatgggcg tgattttcca
    49921 gcttgatcgg atagagtggt tatggctgct tttagctgtt tttctcgttt ggatagtaga
    49981 gatcatcaat actgtttttg aaaatgtagt ggacatgttt acggattttc attttcatcc
    50041 gattgggaaa aaaatcaaag atatggcagc tggagcagtt ttagtgaccg cttgttttgc
    50101 tgtcatcatt gggttgattt tatttgtccc taaaatatat catttgttgt ttaattaaaa
    50161 aggagaacgt atatgccaga acataaatca gggtttgtcg caatcgtcgg ccgcccgaat
    50221 gtaggaaaat ccacattatt gaaccgaatc gtgggtcaaa aaattgccat tatgagtgac
    50281 aaggcacaga caactagaaa taaaatccaa ggagtatata ctacaccaga agctcagctg
    50341 atttttatcg atacaccagg aatccataaa ccaaagcatc gtctaggaga ttttatggta
    50401 gaagcagctt acagtgcgtt gaaagaagtg gatgcagtct tatttatgat cagtgctgat
    50461 caaaaacgcg gccggggaga cgattttatt attgaacgtc taaaaaacgt gcattctcca
    50521 gtttatctga ttatcaataa aatcgataag atccatccag acgagttgct gggaatcatc
    50581 gaagattatt cgacgcagat gccttttgca caagtgattc cgatctctgc tacagaagga
    50641 aacaatgtcg aacgcttaat ggatgtatta gtcagtgaaa tgccagaggg cccacaatat
    50701 tttccagatg atcaagtgac tgatcatcct gaatacttta tcgtttctga attgatccgt
    50761 gagaaagtct tgtttttaac acgagacgaa gtgcctcatt ctgtcgcagt cgttgttgat
    50821 tcgatgaagc gaaacgagaa tgataaaatc catatccagg ccacgatcat cgtcgaaaga
    50881 gatagccaaa aaggaatcat tatcggcaaa ggcggaaaaa tgctgaagca agtaggaacg
    50941 aaagcaagaa aggatatcga aaatctttta ggagacaaag tatttctaga actttgggtc
    51001 aaagtccaaa aagactggcg agacaaaaaa gtctatctgc aagactttgg ctaccgaaaa
    51061 gacgaatact gatattctga acttcttata ggttaagtat agaaataaac tggataaaaa
    51121 tccgcacgtt gagaaaaacg gcggaagatc gaaacataaa taatctggat cggcagaatc
    51181 tgcttttcca gatttatttt ttaggaggga gaatatgagc cagcttgcag aatcgaaagg
    51241 cttgatttta tctagccgta attacaaaga aaaagataaa ttagtcaaaa tctttacgga
    51301 atcttctggc aaaatgatgt tttatatcaa aggaatccat cggcaaaatc agccattagc
    51361 tccggctttg ctccccttta ccgaagcagt ctatatcggt aattttcgaa gtgaaggttt
    51421 gtcgttttta agcagtgtca aagacgtcca tgcgtttcgg aacatccaag aagatatctt
    51481 tttgagcggt tatggtacct atttgctaaa tctagtagat gcagcaatcg aagatcatca
    51541 atatgacccg catttattcc aatttacgca acaagctctg cagcggatgg atcaaggaga
    51601 tgatgcagaa atcatcacga atatctttga agttcaaata ctgcaacgct tcggaatcgc
    51661 gccaatttgg acgcactgtg tcgtatgcgg ggaaacaaaa ggaaaatttg actattcttc
    51721 caaatctggc ggtgtgatat gcgagaaaca ctggccaatg gacgaacacc gttatcatgc
    51781 agatcctcgc gctgtttatt tcgttcgaat gttttcggct atttcttatg ataagatcag
    51841 tggaatccac ttgaaagaag aaacgaagca ggcaatccgt gacctcattg accagttgta
    51901 tgatgaatat gtcggtattc atctaaaaag caaaaagttc attgatcaaa tgaagtcttg
    51961 ggcacacgta ttaaagacgc cagagaaaaa tatgcctgaa aaagagatac ctgaaaaaga
    52021 agaataaatg aaagatacaa caaaaaaatc aaaactattt gacaaataca gggacttccg
    52081 tctattatta accgtagatt aaatagatcg ttcactgaga ggatctatta tataaacaag
    52141 aaaactgcaa aatgaatacg tacattgacc aagatcagta aaaaacacgt ggtttagcga
    52201 gttcgggaga gtggaagccg gacccaaacg atttttgaag aggcacttgc gagtacgata
    52261 aatgaagctg ccgaatcttt attcggaagt agggtggaac cgcgaaatta ttcgtcccta
    52321 tgtctgtaat cagacatagg gactttttgt cgttttcttt cctgtattct tattgacagc
    52381 aacgcttaaa aattgaaagg ggcaaaacag atgagtaaaa aattgaccgt ccaagatatg
    52441 attttaacat tacaaaaatt ttggtcatca aacggctgca tgctgatgca agcttatgac
    52501 acagaaaaag gagcaggtac gatgagccca tatacattct tacgggcaat cggaccggaa
    52561 ccatggaatg ctgcttatgt agagccatcc cgccgaccag ctgacgggcg ttatggagaa
    52621 aacccaaacc gtttgtatca gcatcatcaa tttcaagtcg taatgaaacc ttcacctgaa
    52681 aatatccaag agctttattt agaaagcttg cgtcttttag gaattgatcc attagaacac
    52741 gatattcgtt ttgttgaaga caactgggag aacccatcga tggggtgtgc cggtgtgggt
    52801 tgggaagtat ggcttgatgg gatggagatc acacaattca cgtacttcca gcaagtcgga
    52861 ggactacctt gtaaaccagt tacttctgag atcacttatg gactagaacg tcttgcttca
    52921 tatattcaag aagttgaaag tgtctatgat ctggaatggg ctgatggtgt gaaatatggc
    52981 gagatcttca aacaaccaga atatgaacat tccaaatatt cttttgaagt aagtgaccaa
    53041 gacttgctac taagcaactt tgaccgtttt gaaaaagaag ctaaacgctg tatcgatgaa
    53101 aatcttgttc atccagcgta tgattatatt ttgaaatgca gccatacatt caatttatta
    53161 gatgctcgcg gtgcagtatc cgtaacagaa cgagcaggtt atctttcccg tatccgcaac
    53221 atggctcgtg ctgtagccaa aatctttgtg gcagaacgcg aaaaattagg attcccttta
    53281 ttgaataaaa acacagcaaa tccagaaaac atgaaggagg ctgaataaga tggcaaaaga
    53341 cttattactt gaaatcgggc tggaagaaat gcctgctcat gttgtcacac caagcatgaa
    53401 acaattagaa gaaaaaacag caaaattttt agatgaaaac caattgactt atgatacgat
    53461 cgaaacgttt tctacaccgc gccgcttagc aatcaaagta acaaacattc cagaacgtca
    53521 agcagacagt gaagaagaag tcaaaggacc agccaaaaaa attgctttgg acgcagatgg
    53581 aaactggacg aaagcagctg aaggatttgt ccgcggacaa ggtctgacga cagatgctat
    53641 cacttttaga gaattaaaag gcgtggaata tgtctatgta acgaaataca ctcacggcaa
    53701 agaagcgaaa gaagtattga caggtttagc agatgtcatt aaaagcctca ctttcccagt
    53761 aacgatgcat tgggcagaat acgattttga atatatccgc cctattcact ggatcgtggc
    53821 attgttagat gacgaagtaa tcccgtttga gatcttagat gtcaaaacag gcagagcctc
    53881 tcgaggacat cgtttcttag gcgaagatgt gacatttgcg catgcaaaag aatatgaaga
    53941 aaaattgaat gaacaattcg ttattgcttc accgcaaaaa agacaagaaa tgatcgttac
    54001 acaagcaaat gaattagccg aaaaaaatca ctgggttatt gatctagacg aagatttact
    54061 agaagaagtc aacaatttag tcgaatatcc aactgtcttt tctggcgact tcgaagaaaa
    54121 atatctttct gttcctgaag aagtactagt cacttctatg aaagaacatc aacgttattt
    54181 cgaggtacgg aacgatcaag gtatgctgct tcctcatttt atttctgctc gtaatggtga
    54241 tcgggtaaaa ttggaaaatg tggttcgagg caatcaaaaa gtattgacgg ctcgtttaga
    54301 agatgcggaa ttcttttata atgaagacaa gaaagcttca atcgaccaat acgttgaaaa
    54361 actaaaaact gttactttcc atgaaaaaat cggtagtatc tatgagaaaa tgcagcgggt
    54421 acaagtcatt gcccgtatca ttggcgaaca agtaggacta tctgaggaag aattgatgga
    54481 tctgcaacga gctgctgaaa tctataaatt tgatctagta accaacatgg taggggaatt
    54541 tcctgaacta caagggatca tgggcgaaaa atatgctctg ctgaaaggcg agaaacctgc
    54601 tgtagcaaca gcgattcgtg agcattacat gcctatatcc agtgaaggag agctacctca
    54661 gtctacagta ggttctgttt tagcgattgc agataaatta gatagcattt tttctttctt
    54721 tgcagttggg atgattccaa gcgggtcaaa cgatccttat gccttacgtc gccaagcata
    54781 cggtgtcgtt cggatcatcg aatcgaaaaa atgggccttt cctttgtctg ttctgcaaga
    54841 aacgattagt gatgttatca gtaaagatac ggatcgtttc ggcattggtt tatcagaagg
    54901 gcaacaacag gtgatcgact ttatcaaagg ccgtctgcgc caattattga caacgaagaa
    54961 tattcgtcat gatgtcatcg aagctgtttt gaatgcagaa caaaaagatc tgacaaaagt
    55021 atttgcagca gcacaattgt tcaagcagca tcttgcagat gaagacttca aaccatcgat
    55081 ggaagctttg acacgtgtcg tgaacttagc gaaaaaagcg gagcttgaaa aacaatcaga
    55141 agtagaccct gaactgttcg aaaatgaagc ggagaaagaa ctgcacaaag cagtagaggc
    55201 aatgaaaaaa agctttgctg accaaacgat caacgaaaat tatgatcagc ttgtaagcct
    55261 tcgcccactg atcgaaaatt attttgatca gacgatggtc atggcagaag aaccagcagt
    55321 acgtaagaac cgattaacgc aattaagtca aattgctgcc atggctttgt cgctagcaag
    55381 tttagatcaa atcatcacaa aataatttct aatatgtaaa taaaccagag aataggcaca
    55441 aaataggcta tttctctggt ttatttgtta taaatataaa taactatacc cgtggtgcta
    55501 gttaattttt tcctaggtat tatacaagta atttgagtcg gtcaatttca actgaattat
    55561 ctaaactaac tgatctactt ttagataatt cagttgaaat ttattggtaa actgtcgctt
    55621 ttctttttaa atggcaaaaa tttttaataa tatatgacaa tttagaaatc aactataaga
    55681 ctgattaatt caaaatgatc ttagaatcat tgtatgaaca ctaaatacta tattgttttc
    55741 tattaatcgt tatataaacg attatgaatt caaagctact ttttatgacg tcaatcagag
    55801 agtgtaaaa
//
LOCUS       DAFCNS010000009        52992 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFCNS010000009 DAFCNS010000000
VERSION     DAFCNS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.009"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..312
                     /locus_tag="LNE23_000528"
     CDS             <1..312
                     /locus_tag="LNE23_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBK6930405.1"
                     /translation="QETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKES
                     SGSYTATNGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGW
                     Y"
     gene            complement(396..1118)
                     /locus_tag="LNE23_000529"
     CDS             complement(396..1118)
                     /locus_tag="LNE23_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK6930406.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQSYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKTVPIDHLLIETDGLHAAEWALGGIFSEKYYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1108..1845)
                     /locus_tag="LNE23_000530"
     CDS             complement(1108..1845)
                     /locus_tag="LNE23_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6930407.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYRNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1857..2849)
                     /locus_tag="LNE23_000531"
     CDS             complement(1857..2849)
                     /locus_tag="LNE23_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6930408.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQETKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDITYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDNPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2864..3607)
                     /locus_tag="LNE23_000532"
     CDS             complement(2864..3607)
                     /locus_tag="LNE23_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6930409.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3681..3785)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3848..5170)
                     /locus_tag="LNE23_000533"
     CDS             complement(3848..5170)
                     /locus_tag="LNE23_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6930410.1"
                     /translation="MKTYDAIVIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYFVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANVVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRNPPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5157..6122)
                     /locus_tag="LNE23_000534"
     CDS             complement(5157..6122)
                     /locus_tag="LNE23_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6930411.1"
                     /translation="MTKLIEFKHVQKKYDRKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFNEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6119..7657)
                     /locus_tag="LNE23_000535"
     CDS             complement(6119..7657)
                     /locus_tag="LNE23_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBK6930412.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKVLNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8073..8360)
                     /locus_tag="LNE23_000536"
     CDS             complement(8073..8360)
                     /locus_tag="LNE23_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930413.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8494..9162
                     /locus_tag="LNE23_000537"
     CDS             8494..9162
                     /locus_tag="LNE23_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6930414.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9448..10188)
                     /locus_tag="LNE23_000538"
     CDS             complement(9448..10188)
                     /locus_tag="LNE23_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930415.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKGNLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKMLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10318..11265)
                     /locus_tag="LNE23_000539"
     CDS             complement(10318..11265)
                     /locus_tag="LNE23_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6930416.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQIRSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11394..12158)
                     /locus_tag="LNE23_000540"
     CDS             complement(11394..12158)
                     /locus_tag="LNE23_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6930417.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRGSEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12162..12551)
                     /locus_tag="LNE23_000541"
     CDS             complement(12162..12551)
                     /locus_tag="LNE23_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930418.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12685..13227
                     /locus_tag="LNE23_000542"
     CDS             12685..13227
                     /locus_tag="LNE23_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBK6930419.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13375..14007
                     /locus_tag="LNE23_000543"
     CDS             13375..14007
                     /locus_tag="LNE23_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930420.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14141..14266)
                     /locus_tag="LNE23_000544"
     CDS             complement(14141..14266)
                     /locus_tag="LNE23_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6930421.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYSILWEELAA"
     gene            complement(14400..15296)
                     /locus_tag="LNE23_000545"
     CDS             complement(14400..15296)
                     /locus_tag="LNE23_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBK6930422.1"
                     /translation="MNYYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKK
                     EFSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLS
                     VDEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRATAGNEKYRFITLSSIEHF
                     PLTLANYFFVQKNQLPVTKAFKELAELIGDVNEAYFFDQIEVIVDRIRKNKYKNRRPS
                     IFHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEETAFLKRQIFSELS
                     KKFIPHAFSYIKEYRTVLVSKT"
     gene            complement(15478..16404)
                     /locus_tag="LNE23_000546"
     CDS             complement(15478..16404)
                     /locus_tag="LNE23_000546"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBK6930423.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16489..17250)
                     /gene="pflA"
                     /locus_tag="LNE23_000547"
     CDS             complement(16489..17250)
                     /gene="pflA"
                     /locus_tag="LNE23_000547"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBK6930424.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGNKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17494..19725)
                     /gene="pflB"
                     /locus_tag="LNE23_000548"
     CDS             complement(17494..19725)
                     /gene="pflB"
                     /locus_tag="LNE23_000548"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBK6930425.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20002..22452)
                     /gene="parC"
                     /locus_tag="LNE23_000549"
     CDS             complement(20002..22452)
                     /gene="parC"
                     /locus_tag="LNE23_000549"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBK6930426.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     VTIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22464..24509)
                     /gene="parE"
                     /locus_tag="LNE23_000550"
     CDS             complement(22464..24509)
                     /gene="parE"
                     /locus_tag="LNE23_000550"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBK6930427.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLTVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEVPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24675..25109)
                     /locus_tag="LNE23_000551"
     CDS             complement(24675..25109)
                     /locus_tag="LNE23_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBK6930428.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEVADDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25236..25874
                     /gene="plsY"
                     /locus_tag="LNE23_000552"
     CDS             25236..25874
                     /gene="plsY"
                     /locus_tag="LNE23_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBK6930429.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26006..26881)
                     /locus_tag="LNE23_000553"
     CDS             complement(26006..26881)
                     /locus_tag="LNE23_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBK6930430.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNREIFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADAVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(26963..27754)
                     /gene="codY"
                     /locus_tag="LNE23_000554"
     CDS             complement(26963..27754)
                     /gene="codY"
                     /locus_tag="LNE23_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBK6930431.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27772..29172)
                     /gene="hslU"
                     /locus_tag="LNE23_000555"
     CDS             complement(27772..29172)
                     /gene="hslU"
                     /locus_tag="LNE23_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBK6930432.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALIKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29186..29734)
                     /gene="hslV"
                     /locus_tag="LNE23_000556"
     CDS             complement(29186..29734)
                     /gene="hslV"
                     /locus_tag="LNE23_000556"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBK6930433.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29771..30676)
                     /gene="xerC"
                     /locus_tag="LNE23_000557"
     CDS             complement(29771..30676)
                     /gene="xerC"
                     /locus_tag="LNE23_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBK6930434.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30874..32250)
                     /gene="trmFO"
                     /locus_tag="LNE23_000558"
     CDS             complement(30874..32250)
                     /gene="trmFO"
                     /locus_tag="LNE23_000558"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBK6930435.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLDSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32219..34297)
                     /gene="topA"
                     /locus_tag="LNE23_000559"
     CDS             complement(32219..34297)
                     /gene="topA"
                     /locus_tag="LNE23_000559"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBK6930436.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDIVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34419..35276)
                     /gene="dprA"
                     /locus_tag="LNE23_000560"
     CDS             complement(34419..35276)
                     /gene="dprA"
                     /locus_tag="LNE23_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBK6930437.1"
                     /translation="MYQIEENLLKLAYAKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITTHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVVEAGYVIVSGCAKGIDTYGQQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHRFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35332..36099)
                     /locus_tag="LNE23_000561"
     CDS             complement(35332..36099)
                     /locus_tag="LNE23_000561"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBK6930438.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36092..36952)
                     /gene="ylqF"
                     /locus_tag="LNE23_000562"
     CDS             complement(36092..36952)
                     /gene="ylqF"
                     /locus_tag="LNE23_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBK6930439.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37213..37611
                     /locus_tag="LNE23_000563"
     CDS             37213..37611
                     /locus_tag="LNE23_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6930440.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWVMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37890..38390
                     /locus_tag="LNE23_000564"
     CDS             37890..38390
                     /locus_tag="LNE23_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK6930441.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRVIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(38473..39024)
                     /gene="lepB"
                     /locus_tag="LNE23_000565"
     CDS             complement(38473..39024)
                     /gene="lepB"
                     /locus_tag="LNE23_000565"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK6930442.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39176..39403)
                     /locus_tag="LNE23_000566"
     CDS             complement(39176..39403)
                     /locus_tag="LNE23_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBK6930443.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39400..39918)
                     /gene="msrA"
                     /locus_tag="LNE23_000567"
     CDS             complement(39400..39918)
                     /gene="msrA"
                     /locus_tag="LNE23_000567"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK6930444.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(39938..40651)
                     /locus_tag="LNE23_000568"
     CDS             complement(39938..40651)
                     /locus_tag="LNE23_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBK6930445.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40654..41550)
                     /locus_tag="LNE23_000569"
     CDS             complement(40654..41550)
                     /locus_tag="LNE23_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK6930446.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41708..42550)
                     /locus_tag="LNE23_000570"
     CDS             complement(41708..42550)
                     /locus_tag="LNE23_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6930447.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEEILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42682..43335
                     /locus_tag="LNE23_000571"
     CDS             42682..43335
                     /locus_tag="LNE23_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBK6930448.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43399..43926)
                     /locus_tag="LNE23_000572"
     CDS             complement(43399..43926)
                     /locus_tag="LNE23_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK6930449.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEIDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(43946..44893)
                     /locus_tag="LNE23_000573"
     CDS             complement(43946..44893)
                     /locus_tag="LNE23_000573"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBK6930450.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKEVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(44905..46791)
                     /locus_tag="LNE23_000574"
     CDS             complement(44905..46791)
                     /locus_tag="LNE23_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6930451.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSGDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(46956..49028)
                     /locus_tag="LNE23_000575"
     CDS             complement(46956..49028)
                     /locus_tag="LNE23_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBK6930452.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRHADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            complement(49041..49316)
                     /locus_tag="LNE23_000576"
     CDS             complement(49041..49316)
                     /locus_tag="LNE23_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930453.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49678..50133)
                     /locus_tag="LNE23_000577"
     CDS             complement(49678..50133)
                     /locus_tag="LNE23_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930454.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50287..51495)
                     /locus_tag="LNE23_000578"
     CDS             complement(50287..51495)
                     /locus_tag="LNE23_000578"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBK6930455.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPSLKEEGEALLRFAELPSVPLKEESQVWTLLISQLGLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51607..51939)
                     /locus_tag="LNE23_000579"
     CDS             complement(51607..51939)
                     /locus_tag="LNE23_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK6930456.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52087..52962
                     /locus_tag="LNE23_000580"
     CDS             52087..52962
                     /locus_tag="LNE23_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6930457.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
BASE COUNT        14535 a        11098 c         9088 g        18271 t
ORIGIN      
        1 caagaaacgg ttcaaacgga aacacaagca gcgcctgttg ctgaaacaca acctgctcct
       61 gctgtaacag aaactgctgc tactccagca agtacaagct ctgcaaaaga atggatcgca
      121 caaaaagaat caagcggttc ttacacagca acgaacggac gttacatcgg ccgttaccaa
      181 ttagattctt cttacttaaa tggtgactac tctgctgcaa accaagaaag agtagcagaa
      241 caatatgtta cttcacgtta tggttcttgg gaagcggcga aagcattctg ggaagcaaac
      301 ggttggtact aagattcatt tattaatcga acggtttact tttatacaaa gaaagccaaa
      361 aagaggtgtg acacaactag tgtcacacct cctttttatt tacctaatct aatgaaacgt
      421 tccaagtttt gataagtatt tttcttaact tcttcaactg atagctcttt gatctcagcg
      481 attgtcttta actgttcttc aagcagtgag acataatatt tttctgaaaa aatcccccct
      541 aaagcccact ctgctgcatg tagtccatcc gtttcaataa gtaagtggtc gattgggact
      601 gttttagcta gttgccagac tgctgggttc tttttcaaat caagaccaat gctgaaataa
      661 cagcctaaat caatatagtc ttgttgatag tccaaagaag aataccaatg aatcaaatag
      721 tgattagggt aagattggat ataatttaag atctcttttt cgcatccttt tgtatgcagt
      781 acaacaggtt tctcatttat ggctgcaaaa gcaagctgtc gctcaaatac ttttttttgt
      841 gtagtcatcg gtacattcgt ccaaatatta tctaacccta tttcaccgat gatccttgct
      901 tttttcaaaa aaggctcaac ttgttcaaac gtatagctat ctgctttcca aggatggata
      961 ccgtaactta aggcttgagt cttacttgct gcaagctgtc tgttttcctt ccattcttct
     1021 ggcgaatcac aattgatgat cgtcaaaatc gtgtgttcct gctgaaggag tgctaaactc
     1081 tgactcccat ctaaatgaca atgagcatca cttaacatct aattcacctg ccaataactg
     1141 ataaatcgtt tgcttcaact ctgattgctg cagtagccat tcacgtgacc gtcgtttttc
     1201 aggcacttga atctcagcaa tcacatgtgc cggacgggcg gaaagcacaa gaatcctgtc
     1261 tgataaataa attgcttctt cgatatcatg cgtcacaagt aaagtagtcc gctttgcttt
     1321 tttaggcagc ttcaacatcc agtcttgcat ctgccctctg gtcatagcat ctaacgctcc
     1381 aaatggttca tcaagaagca agtaagaaga aggattcatg attgttcgta acaaggccgt
     1441 tcgctgcttc atcccgccag ataattgttc cggcaaaaaa tgttcatatc cgtttaaccc
     1501 aaagatatcc agataatccc ttacttgttc ttcattgatt gttagatgat tgattttttg
     1561 atataacgta acgtttcgat agacgctcag ccaaggaagt aacatatctt cttgcggcat
     1621 ataactaaat gctgtatgga gaccagttac tttttgatcg tctactttga tcgttccaga
     1681 agtcataggc tggactcctg ttaaagtttt cagcaatgtt gacttaccgg ctccgcttgg
     1741 cgcgacaatc gatgtaaagg tatctttagg gatagtgaaa tggatgtctt ctaatatttt
     1801 caggttttca atgactacat tcaagtggct gatttggatt gacatatcta tcactcctat
     1861 tttataaact gattagtgaa tagttcttgc gaaggtacat cttgcttgat caatccgttt
     1921 tcctgcataa aagctgtata attgcgccaa actttttctt ccatcaatcc ccaattctca
     1981 ggattatccg tataattttt tgacaagaat gcttgtgatt cttctaaaaa cgtccgatca
     2041 ttttcttttg catatttttg caacagttta gcactgtcat ttggatcctt aatagcttcc
     2101 tgatagcctt ttttcgtggc atccataaaa gactgtacca attctggatc acttttgatc
     2161 agttgatcat tcgtaataat taccggagta tagtaatcta accgttcatc taattctttc
     2221 aatgggatat agtttagttc aatacccgct tcttttgctt tgatcatatc ccaaccttca
     2281 aagtaccatt ggatatctga ggattttcct aagctttctg gcccttctcc attgcttcct
     2341 accattgtca gttttgaaaa gtccccgccg tctttttcca tcacagcttt taagacagct
     2401 tcttcacttg gactttgcca acctgcatat gtttttcctt caaaatcttt tggtgaatgg
     2461 atgttactgt ctgatagggt agcgaatcca gaagtattat gtttgatcac tgtagcaatt
     2521 gctttgactg gtatattctg tccatctgcg tgagcataag tgatatcttc ttgataactt
     2581 acaccaaatt gtgctttgtc cgcacctact agaccaatac ttgtcgaatt atctcctggt
     2641 tcgatgattt ggacatttaa accagcctct ttataatagc ctttttcttt tgctaggtag
     2701 atgccagtat gattagtatt cggtacataa tccaaaacaa gtgtcacttt tttcaaatcc
     2761 tttgtttctt gtttgctact tgtttctttt gcagtgccac aagcggataa aagcaacagt
     2821 agcggtaata aaagccataa ttttttcatt tgatcccctt ctcttaaaaa atataaagca
     2881 tacgataata cccttttttc aataatgaac aagcgccatt cagcaataaa ctcaatagaa
     2941 tgacacaaat gatcgcagca aaaactttat cgatctggta gctgtttttt acgcgtatca
     3001 tgtaataccc taagcctgct tgagcactta gccattctcc aacgactgct ccgctgacac
     3061 aataagtagc agccactttt aaaccggaaa agaagccgga taaacccgct ggtattttat
     3121 atattcgata aatcttccct gtatctgccc ccatcgtttt caaaaagatg atttgctctt
     3181 ttgatacttt cctcaatgta tccgaaaaag aaactacgat cggaaaatag cttatcaaga
     3241 caacaaggat cacttttgga agcataccga aaccaaacca tagggtaagt aatggcccta
     3301 acaccatgat tggcaacatt tgtgaaatca ctagtaacgg ataaaaagac atttctaaaa
     3361 acttccaata atccattgcc aaggcagtaa taaaagaaag aacgctagct attaccaaac
     3421 caagcactgc ttcatttaat gtcgtgacag aatgggaaag taatacttgt gcatctgccg
     3481 ctaatgcaga aaatacagca gcaggtccgg gtaaaataaa ggcaggtatt ttccaaacta
     3541 tgacaacaat ctgccaaata attaaaatga atgccatact gactgctgga taaagatttt
     3601 tcttcatttt gttacctcct ttcttctaat atgaataaca aaaaacgctt gaaatgggca
     3661 cgacatagta acccgcttca agcgtctgct atgtttctcc cttcaccagt attaactgac
     3721 aggttcaatg ggtctaagtt cacaacttac tctcatgtcg tctacgacat ccccagaaac
     3781 aggtattcat tttttaaagt gtagcacaag aaggaaaaag caacaagcgc tttagttatc
     3841 atgctgatta tttcaataaa taggagagat cacttgccaa tgtcggatac cccatgatat
     3901 agctatccaa ctctttttta gtcaattttt gattgatgac tagtgtcaaa tcatcgatca
     3961 attcgtctgc ttgctcgcta atcactgcag caccaatcaa ataattttgc tggtcaaagg
     4021 tcaattcagc cttagcaact ggttcattga cacgatgata ggtgaaccaa ctggtaagat
     4081 ccatctctac cacttgatct gacgaagaag cgtgactttt tgtaacacct acttcagcaa
     4141 gtttaggact ggcgtagacg atcgtaggta taatcggata tttgataggt tcgcttgttg
     4201 catccgttat ccgcttagct acataatttc cttcaaaagt ggcgactggt gtcaatttgg
     4261 gggggttcct tgaaacaata tctccacacg caaaaatttt tggattgctc gtttgcagat
     4321 aatcattgac tgcaattccg tgtttatcaa agacgacatt cgcttgctca agtgctaaag
     4381 attctgtatt cggctgtctc ccagtcgcac aaaaaatcat atctgctact aattccgtct
     4441 cttttccgac taaacgatag cgagtacctt ctgaaatgat cttttgggtt tcaacaccaa
     4501 aagcaaactg aatgccagat gcttccattt gatggacggc ttcctctacc aaagacgcct
     4561 caaattcttt taatggccgt tgattatgat ggacaatggt tactttgctc cctgcagcat
     4621 tggcaatcgt agctagctca aaagcaatat aaccaccacc gataaaaatg atttctttag
     4681 gcagtacagg caatgataaa aagtctgcac tagttttcaa gtattccttt ccctccactg
     4741 gtaagatcgt aggtctttgc ccggttgcca aaacaaagta atccgcatgg aagacttctt
     4801 cgttgacttc aatagatgaa tcatccaaaa aacgagcggt tcctgaaaga tgatcgattt
     4861 cagcttctgc taattgtttt tttcggcttt ctgggactgg atccgtaaat gttcgtttga
     4921 atttttgaag ttcttcccag ttggcagttg ggatctcgtt aaaacctttt ccactcaatt
     4981 gttttacccg attacgtgcc tctactgcac ttaacaagac ttttttagga tcacatccac
     5041 ggttaggaca agtcccgccc catagatctt cttcgaccac tagtactgtt ttacctgctt
     5101 ctttcaatcc ataagcagca cttaagccgc tcacacctga accgatgacg attgcatcat
     5161 atgttttcat ttccttcctc ctctcgactt aaaaaagcaa agatttcttt acttgtcagc
     5221 gacccaatac ttctttgctt ttctactatc acaatgcttg gatacaccga aagaagcgaa
     5281 tatacttctt tcataggcgt atcaaagcaa acttgttgac gagcattttc tggtatttca
     5341 tttaagaaat atccattgac gactaaatct tctactcgcc actctttcgt ctgtttcggt
     5401 tcattaaaga aatgccgaac gtagtcgttt ttaggattca tcaataactg ctctggtgtg
     5461 tcacattgga taatttctcc atccttcata aatgcaatac gatcacctaa cttgattgct
     5521 tcttccatat tgtgcgtaac gaaaacgatg gtcgtgccca attcttcgtg taaggacaaa
     5581 accaattctt gtaatgagtt tcttgaaatc ggatctaatg cgctaaatgg ctcgtccatc
     5641 aaaatcacat ctgggcttgc agcaatagct cggatgatcc cgatccgttg ctgttctcct
     5701 ccagacaact ctctaggcat acgatctcga taaatatcag gagataagcc aactttttga
     5761 agaagttcat ctacacggtc tgctcttttc tgcttttccc aacctaacat ttcagggatg
     5821 acttcaatat tttgcttgac tgtcatggtc gggaacaaag cgatttgctg cagcacatat
     5881 cccatattcc accgcatttt ctgcagatta tattcattta acgattttcc tttgaagtag
     5941 atgtctcctg ctgatggttt agataatcca ttgatcattt tcaaagtagt tgtttttcca
     6001 ctaccggaag gaccaaccaa aacaaagatt tctccttttt tgattgccag atttaggtca
     6061 tcgatgacat actttctatc atactttttt tgcacatgtt tgaactcgat taatttagtc
     6121 actgatcttc ctcctttact aatccttcat ttttcaggaa gattcgtgct acttctgtcg
     6181 gctttttctt ttcgacattg acttggtaat tcatatcaat catttgatcc tctgttattt
     6241 ttccgcttaa tttattcagt actttaacga tttcaggatg ttctttagca aaatcttttt
     6301 tcatcagcgg tgcgccttga taagctggga ataaccctcg atcatcttct agagtcacta
     6361 gatcgtactc cttgagctgg ctatctgtgg aataggcatc cacaatattg acatctccat
     6421 tattgattgc ttggtaacgc aaacttggct ccatgctttg tacacttgaa aaattcaagc
     6481 catatgcttc ttggattcct ttatatccat ctgaacgatc aataaattcc agtgtgaatc
     6541 ccgctttgat ctgatcagag atagctgcca aatcggaaat ctttttaagt tgatgttcgt
     6601 tcgcaaaaga tctcttgact gccaaggcat acgtattttg gtaagccatt ggttttaata
     6661 atgtcatatc gaattgctta ttcaataatt cttctgcttc gtcatatgtt ttttcttttg
     6721 tcaaatcagc acttttcatt gcttcaggta ccttcaccaa gctttctagg acagtacctg
     6781 tgaactcagg atagatatcg atttgacctt gatccagtgc attgaacaaa aaactcgtct
     6841 tgccaaaatt cggttttaat tcaacttctg tttttgtgtt gtcttcaatc aattctttat
     6901 acatttggat caggatttca ggttccgctc ctaattttcc agcaatcgtg atggtttcat
     6961 ttttaaacaa ggcagactga tataaagaaa cgccgccaaa taaacacacc actgctgcta
     7021 aagtaatgat ggaatatcga ggtttcttat gctgcaacca gcgaatcagt ccgctgaata
     7081 taattgcaag caaggcagaa ctgatcgcac caatcaatgt caaaacaggt gtatttcggt
     7141 caatacctaa caaaataaat gtccctaatc cacctgctcc gatcaatgct gccaaagtag
     7201 ccgtaccgat gattaaaact aaggcagttc ggatacctga gataatgaca ggtaatgcga
     7261 tgggaagctc tactttcagc aagcgtctca ttctggacat accaaatgct accgctgctt
     7321 cttcaataga cgggtcaatt tcagctagac caatatacgt gttttggaaa atcggaagca
     7381 aagcgtaaat aacaagtgca atcaatgctg gaacagttcc gatccctaca aatgggataa
     7441 gtaaacctaa taaggcaagt gaaggaatcg tttggagcac acccgtgatc tgcaataaga
     7501 cttctgccat ttttttatga ttggctgccc aaatcgctaa aggaatagca atcagtgctg
     7561 caatcagcaa agaaatgaga gaaatggaca gatgttgaaa tgcagcttcc agcaactcgc
     7621 cttttctttc acttaatgtt tgtataaaat tattcatgcg accttccttt ctgttattct
     7681 ttaagtataa acatttcttt cctttttccc aactaacttg tcttgcctta accttacagc
     7741 acgaacaaaa aaaaagaaaa aaatacctct aaatatgctg acaaaaaaga atccaagcaa
     7801 caaagtcgga tcaacttttt ggttctcgtt ttttcactac gaataccata aaaatctata
     7861 cgaaattgtc acttggaatc agctatatct tttttacaca cacaactttt attttaataa
     7921 aaagaaacag ccaataaagt agaaatctcg tgggggagaa cacctttatt ggcctgttca
     7981 acgaatatta tactgtatca tgacactttt ttgccaaaaa tgtaaaaaac ggttgttttg
     8041 acatcataaa cgtcttgttt tcttattttt acttatatca ccactttatt ttctatctct
     8101 ttcactccat aaagcggaat gagttcttct tttgactgat aataaacagc tttctcacgg
     8161 ttcttcatca aaaaaagaaa gatgtctttc atagcaagtg gtgctttgcc gtgttcaaat
     8221 aaaaagataa attgatttgc tttatttatc tggaccattg tcacaggcac cagtttttgt
     8281 tcttgagcca aaaaaactcc ttgatactct gtttttttgg gtgattttgt aagaattgtc
     8341 aataagtctg ctgctcgcat ttttccactc cttatttgat aaataaaaag aaacagccaa
     8401 aaataatttt ttttcatttt cctcttgaag taagagtata agtcagtata ataaagaagt
     8461 tgctgaaaat tttcaaaaga aaagaggcta gaaatgaaaa aattaaattt atttacagaa
     8521 ttgtgcggaa tcattttagg cgcaggaatc tacggattag ctgtgacagg aatcaatatt
     8581 ccatctaagc tggcagatgg tggggttact ggtattgctt tgctcttgca taatcttttt
     8641 ggattcgctc cttctgtcac ttcactgctt tttaatttac ctttattgct tatctcattg
     8701 tttctttttg gaaaacacgc ttttctccgg acgatcgtcg gaacatttgc gctagttttc
     8761 tttttacatt tatgggagag ccttcatata cattttgctg tagatagtct actgatcaat
     8821 agtttgatga caggtattct ttctggcatt ggttgcggtc tcgtctttcg attcggcgga
     8881 agcacaggcg gaacagatat catttatcaa gccattgaaa aatattttca tattaagatt
     8941 ggcaaaagtc ttttcttgat tacttttggt attttagttg tctccttgct ttatttggat
     9001 ctcacacatt ttctgtatac attacttagc tgttctattc tatcttatac attgaataaa
     9061 gtgaaatatc tggaaattgg atctgttttg ccccaattgg aagcaaaaca gactacagaa
     9121 acatttgaac cacttgaccc tcttgaacag ttagagcact gattcttatt tttcactact
     9181 ttatttacag aaatatcatt atgtaaacta aaatgatatt attcagtaaa atcttcttta
     9241 taaaaaaata aactattttt gcaaacgatc tgcgaaaata gtttgttttt ttattttgcc
     9301 attattttct tacttattgg gagaaaaaat tcgcttaagc tcaaaaaaat ctgaggatac
     9361 gatataaact tccggataga gtataaaata aggaaacgcc gcagcgtttc ctttccaact
     9421 gtagccgaat ataaatttgt atatcactta tttaaaatag ctattttctc ccaaagagtt
     9481 tccttgatca tcgatcaatc cttgtaatga gcgttctaaa ataccatggc tttttttcga
     9541 ttttttttct tctgactgct ttgtttcctc taacattttt gttcgattga atgttcgtac
     9601 agacggctca ccatttcttt tttcttgata cggcgtcggt tcagtatcga acaacagata
     9661 agagtcatcc gtttttttca tcgattcgat taaatgatcc tcagaaattt tgctttctct
     9721 agggtctggg ataatcgatg cgggtacata tttaggaaca aaataagaac gctcccgcac
     9781 cttcgtgtca acagatgcgt ttcgtgtagt atgcttggac tctgtccttg tttcactttg
     9841 tctgacagaa cgagaggaat caccaaaaag tttttttcgt cctgtcgatg tgatttctga
     9901 ttggtaatgg ccggtataat ccggtaattt tcctttatgc ttttccaaat ttcccttacg
     9961 cggagctgac ttcaatccat ttgttgcacg acgcttgcga cggattttct cggtgttcgt
    10021 gttcgtgaaa tgaaattctt tcatctgctc aaacggggac tgattctgtt cagtcaaaag
    10081 atcttcttgt ccgtcaaata atactcgctt cttgtcgttg gttaatttta cgcctaactc
    10141 atcatcgtac gcaggaaaac ggaaatgttt tcgtctgatt tctttcattc aaaccatcct
    10201 tctcaaaaat ggaacttttc tcaataatag cacagattca ttgttttcag tagcaatgcg
    10261 ctgattattt taagaaaata gcgatgaatt tcataaatat atatatagta ataagactta
    10321 attcgttgtc tctctgataa ctagttcagg aaaaaaacga cgataacctt gactcgcccc
    10381 tttttcttct atttttttta acaacatttc tccacatact tcacccatac gtataatatc
    10441 ttgcttgatc gttgtcaatt taggtgacct gatttggtct aaaaataccc catcaaatcc
    10501 aatcacacca aattcttcgg gcaagtgtcc tccagcattt ataatccctc ttactgctcc
    10561 tagtgctagc cgatctgaac tacatacaaa acaagtttta tctgtaaaat ctgcccagtt
    10621 ttctgtcata tatctttcta cttctgtcga atgatttgga aaccgcaaga ttttactagg
    10681 aagatcaact tccttcatca cagataaata accagtttcc cttgaaattt caaaaggctc
    10741 gtccacttcg ataccgatat aaataatcgt ttgataatct ctttctaagg catattgcgt
    10801 tgcttttgct gtaccatatt cattatcgct gtctacataa tcatatcctt cagaattttc
    10861 cccaaacaag acaactggtt tttccaaacg atcgatccaa tcgaagtctt tttttctgac
    10921 acctgtgatg atgtatccat cacaagaacc gacgtcgaag gcatcatttg taactaattg
    10981 gagagaataa tgcgctttac cgatccgttt agcgattccc atcagcaaat tcatgtaata
    11041 gggctcagtt gtgtcgattt cttccagtat aaataatttg atgatctgcg accgattcgt
    11101 gacaagtgct tttgcagcaa tatttggacg gtaatccagt tcagccattg ctgagaaaac
    11161 taactctttc aattcgtcag tcacctgttc cggatgattg atgactcgag agaccgtcat
    11221 ttttgaaaca ttggcacgtt ttgcaacatc tgctaaagtg ggcatgctct cttcctttct
    11281 aacatgttgt cattcttact atcaattata ccaaattttc ctaacagagt ttattatagc
    11341 ccaaaataaa aaacggaaaa tcctctatcg tcatgagtga ttttccgttt caattattta
    11401 tactttttta tcaaattttt tgctaaaaaa gtggaagctt cttccattag tggtatttgt
    11461 accatatgtc gctgtgtttt tgaggaacga aaaacaattt cttttccgta agacttatgc
    11521 cgattttttt ctacaaatcg ttccgtttgt atataaggga ttttttcgtc agcttgtcca
    11581 tgccaaaaga acagaggacg tccagctaac ttttccggct gcaatgacaa atcatagctc
    11641 ttaacccatg aaatcaaatc aaaatagtct tttggtacga cataattata ttccgatccg
    11701 cgccttacga tttcttttcc atattccaaa ggtgcagggg aacccatcaa acacacgccc
    11761 gctactatat gaggatgttt agccaacaag gcgcatgttg tcattccccc catcgaaacc
    11821 ccgccaactg caagctgatc atgtagaaga cctctttttt ctaaatgctt gataagtgtg
    11881 tcaaattcaa acagatttcc gtagatacta tcccaaaatg tataggatgg aatagaagaa
    11941 accggctgtt tgcgctcccc atgattcatc gcatctggca ggatcacacg aatgccctgc
    12001 tgcgcaattt ttcttccttg cgtcaaaact aactccttac ttgtctgcca tccgtgataa
    12061 tagatgacaa cgggaatacg ttcctctctt aattctttca gaacaacttc taacactgga
    12121 atatgatgga cagaacgttt caatataatt ggattcaatt gttactcacc tattttcatt
    12181 aaatttcctg atcgccatag caaccatgct ccaacagttg tctggaacag gttcagtgta
    12241 ccaaataata taaatgcagg actgaatggc tcgggaaata agcacagaga acaaagtccc
    12301 accactccta atgctagcga aaggagtgga taaatagaaa aattcaaaaa actggcaacg
    12361 ctaccgaaaa tattaccaat aaacagtatc aaaaagaaaa aaacgcttaa aattaaacca
    12421 tctgtcagcg acatctgaaa aacaatgttc tctacctcaa ttggctgttg aatcgaatag
    12481 acacctaagt atatactgag aaggagactt agcacgtggc tggcacaaaa cacacgattg
    12541 accgttttca ttttaatcac ctaacctatc tttataattc tattataatc attttatcat
    12601 tttttgtatg tataaagttt caatttttct tcacttacga ttttttttga tattgttgtt
    12661 atataaatga aaggagtttt acaaatgatt gatccaaaaa tgttggggat gatcttcctc
    12721 attaattttg cttatatcac tctgaacacg ttacgtttca tgttgacaat gaaaggctat
    12781 cgactgattg ctccacttgt cagtatggtc gagatcacga tttatatcgt cggcttatcc
    12841 cttgttttag atcgtttaga taatccactg aatttatttg tctatgcgtt aggttacgca
    12901 gtaggtatca gtgtcggaat tttgattgaa gacaaattgg cattaggata catcatggtc
    12961 acaacgattt tgccagcaaa tagcgcagaa gataaaagtt tgccgggtat actgagagaa
    13021 cacggttatg gtgtgacaca gacttctgca atggggctgg aaggtgagcg gctagttctc
    13081 gaaatacttt ctcctagaaa aagcgaacgt gaactgtatc gcctgatcaa agaaatcgaa
    13141 gagcgtgctt ttatcatttc ttatgagcca aaatatattt caggtggttt ctggactaaa
    13201 aaagtgagaa aacgacaagg aaaataactc acaaacttaa aaactgtcta atatctaaat
    13261 taaattttaa atgttagaca gtttttattt attattttga ttaaaaaaca tcgacatata
    13321 ctcatcaatt tgttattata ctttatatac ttatcaattt aatttggaga tattatggaa
    13381 attcaacgta gaaccactaa ttttttacat ttaggctctt tttttgcaac tttgacgatc
    13441 ttattttttc tatgttattc ataccttaag acctcacatg cgccttctag tgcattaggc
    13501 tggttgcttt tggatcattc tgatatcaca gaagccgaag aaaatccgtt ttttttgatt
    13561 ttgtcttctc tttgcggctt actgttttct gtttatttcg gtgcctattt ttttcatcga
    13621 catcctggta cgttaaacga agcttcaagc gtattgattg catttaaaac gttttctttt
    13681 ttagcaattc ttttttattc gtttaatcac aatacgcttt tcttctttat catgaatggt
    13741 ttagtcgtga ttttatccat ttttacttat tttatttttt cactcatttt tagccaattg
    13801 aaatgtccac tcttctttcg attagttcct gtcacctgtt ttatttacag cttgttttcc
    13861 ttttttgtcc ttgctttaat gccactgacc aacccttcga cgatccaagt cgcaggaaat
    13921 ctgttggata tgctcttcat cgtcagtcta ggtgtattta tgtggcaaag aaaaaacaaa
    13981 caaaataaaa agcattttga agcctgacta ttacgacttc ataaatgctt ttttagtttt
    14041 aaacacttca atctaaaaga ataacccaag attggataaa ttattaatga ctcgccattc
    14101 gtttcttagt gattctggat catcaaatgg acttcctaat ctaagcagct agttcttccc
    14161 ataaaatgct atacttgact gaatcataat attgattatt taaaaatcta acttttctta
    14221 tcactacttc ctttgtcatt ccacatttct caccattttt ttgcattcct ttgtttccag
    14281 accatgtagt gaatccaata ggtggtagat aggcaaaata ctcaaataat tcttttaacc
    14341 ataaaggaag agaaaggctc ctcctgcaag agtcagtata atttttgctt actgctctct
    14401 taggttttac tcactaaaac tgttctatat tctttgatat aagaaaatgc atgcggtata
    14461 aatttcttgc tcagctcaga aaagatttgt cgtttaagaa aggccgtttc ctccattgtc
    14521 aatgaaggat ccaatgttgg taattcaatc tcaaaattgt ttttttcgac aataggtaat
    14581 tctaatgtga atgattcttc ttcctttata gtattcgtca caagtagaga ctgatggaaa
    14641 atactaggcc ggcgattttt atatttattc ttacgtatcc tgtcaacgat aacttctatt
    14701 tgatcaaaaa agtacgcttc atttacgtct ccaataagtt cggctaattc tttgaatgcc
    14761 ttcgttacag gaagctgatt cttctgtaca aaaaagtaat tagccaaagt aagcgggaag
    14821 tgttctatag aggaaagtgt gataaaacgg tacttctcat ttcctgcagt tgccctcagc
    14881 aattctgtct ctcggctgtt tcttactcca taaaaatacg cttctttccg ttcatcttcg
    14941 aaacaatacg cccaaataac ttctcccatc gctaattttt gttcatcaac agagagtctc
    15001 ttcaattgtt cagctatggt ttcagcagcc gaagtggctt gttgcaagta atctttagga
    15061 tcaaatattg gaaagttcaa atgatatctt cgttcttccc tcttgattaa tcctgcatcg
    15121 atcaatagat caagaaaatg ggaaaattta ttttgcgaaa attctttttt caattctctt
    15181 agaatcgttt cttgttctcg atgttcatat agataatcgg tcaatgaaga aaaaagcgga
    15241 tcatcggtca gtttttgata aaagttttcc ttattttttg aataataata gttcatatat
    15301 gttctctttt ctatccgttt tattttactt aaaaggcttt tacgtaaaaa ggaggaagaa
    15361 caagagtcat aattgactgt tattctcctc catttcttcg gcctttagta tgttctatgt
    15421 ttctttttat atcaatcgct tatccattca ttttgaatgc ttcatactac cctttgatta
    15481 gctaaataca gccgtcaatt gaggtacgac ttgtttttta cgtgaaacga cacctttaag
    15541 caaagcacgg ttgttggtca aagttgtttg gaaagcttct tctacttttt cttttccttt
    15601 accagcaaca agtaattctg aatcactgtc taagatgttt gtgacaacta atacgaacag
    15661 atcgtagcca tttgctttca tttcatcttc cattgctgct tctagctctg cttgtcgatc
    15721 catcacttca ttaagatcca ctgtattgac ttgagcgata cgaacgttgg aactgcccat
    15781 tgggaaactt tttgcatcca aatcaagaag aaccgcagct gatttatcac ttagatttgt
    15841 tcctgctttc aacatttcaa gtccataagt gtttagatcg acttcagcga ttgctgcaag
    15901 ttcttttgca gctgcttcat cttcaggcgt acatgttggt gatttgaaca acaatgtatc
    15961 tgaaatgata gctgagagca tgattcctgc gatttcttta ggaattgtta cttggttttc
    16021 tttatatagt tttaaaataa tcgtacttgt acatccaact ggttctgcac gatagtacaa
    16081 tggattagct gtttcaaagt ttgcaatacg atggtggtct actacagcta aaatatttac
    16141 ttcgctgata tctgaaatac tttgttggaa ttcattatga tccacaagca tgacatctgc
    16201 attttctgct ttagctgact ctactactcg tggtgcagat agctgaaagt ggtctaatgc
    16261 gtattgtgtt tcttcattag gtgtacctag agcaactgct tctgtttctt tacctaattc
    16321 attttgcaag tgtgaaaacg caatggctgc cccgattgca tctgtatctg gattttggtg
    16381 accaaaaact aaaatttttg acatgtcatt cgctcctttg tgatttctcc tatttaataa
    16441 tagcaaaaaa acggaaagaa gcaatactta tcttctttcc gatgcgacct atcgcgttaa
    16501 atattttgta tactcttctg tatgaagcaa tcgtttagca ttttcgacac gttcttttgt
    16561 aggtggttcg atcccttcta atggatattt caaaccaagt gtttcccact tgtattttcc
    16621 catcgtatga taaggaagaa tctctactct gtctacattt gacaaggttt gaataaagtt
    16681 gtctagtcgg atcaaatatt catcataatc acttcgctca ggaactagta catggcgaat
    16741 ccaaaccggt ttgttgattt ttgacaaata ttgagccatt tctaaaatat tttcattgtc
    16801 ccaatgcgtt agttttttat gctcttcatt atcgatgtgt ttgatgtcaa ataacagtaa
    16861 atctgtatac ttcatcaatt cttgaaaacg tgaaaagaaa ggttcttcat acgtaaaagg
    16921 ttttccgcat gtatccaatg tagtatgcat cttccgctct tttgcttttt taaatagttc
    16981 gatcaaaaaa tcgatctgca atagaggttc tccaccacta acagtaatac cacctttatt
    17041 tccccagtaa ggtctgaagc gttctgcttt atctaaaagt tcatctgctg tgtattcttt
    17101 ccctccgcca atattccaag tatctggatt gtgacaaaat tggcaacgca tgcggcatcc
    17161 ttgcataaac acgacaaagc gcagtccagg tccgtctact gaaccgaaag tttcgataga
    17221 atgaacatag ccgattgttt tttcttccat ttaagttcac atcttttctt tgatttcatt
    17281 ttaagtacaa gttagctgtc gaagcgaagc tttctgaaca aaatttttcg caattgccgc
    17341 ttcgttatgt gtaaaattta cagggacgga acatacaagt cctgcccctg taaatcgttt
    17401 ctttcaaata agcatactga ttattcgatt ttgatgcgat ggctattcaa cgtcctttcg
    17461 acgctaagtc gaaaaacttt tttcacgaac tttttacatt ctgtcgtgtg aagttctcat
    17521 caagacatct aattgttgtt ctcttgtcaa gtcacggaat ttaactgcat aaccagatac
    17581 ccggatcgtc aagttcggat atttcattgg attttccatt gcatcacgca ataaatcatt
    17641 actgaataca ttgacattca aatgataagc tccttttgag aagtatccat ctaagacatt
    17701 acgcaagttt tcgattcttg tatcttgttc ttttccaaga cctgaaggat tgattgtttg
    17761 ggtatttgaa attccatcca atgcacagcc gtaattcatt ttagctgttg agttcaggct
    17821 agctaataac ccactttgtt ccgcattgta acttggattt gctcctggcg caagtggttt
    17881 tccagcttca cggccatcag gagtatttcc tgtattttta ccgtaaacaa catttgaagt
    17941 aatcgtcaat aatgaagttg ttggttttga gttgcggtaa gttttatgac gtttgatttg
    18001 tcccatgaag tattccaaga tccatgaaac taattcatct gcacggtcat catcatttcc
    18061 gtatttaggg aagtcgccat ccacttcaaa atcaactgct attccttttt cattacggat
    18121 tgcttttact tttgcatatt tgatcgctga caagctgtct gccgcatgag aaatcccagc
    18181 aatacctgta gcaaatgtgc gttgcaagtc agaatccatc aaagccagct gagctgcttc
    18241 ataagaatat ttatcatgca tataatgaat gatgttcaac gtattgacat atagttctgc
    18301 taaccaatcc atcatattca tgaagttatg tttcacttct tcaaagtcta gtatttctga
    18361 tgtgattgga cgatattctg gtccaacttg ggcgccagtc acttcatcga ctccgccatt
    18421 gatggcatat aatagtgttt tagccaagtt agcacgagca ccaaagaatt gcatgtcttt
    18481 tccgacaact gttcctgata cacagcaagc aatcgcacaa tcatcagaac cccattgttc
    18541 acgaagctga ttatcatttt cgaattgaat cgaagaactt tcgatagcga ttctgctagc
    18601 atatgttctg aatccttctg gaaggtgttc agaatacaaa acagtcaagt ttggttctgg
    18661 tgaaggtccc atatttgtta atgtatgaag cagacggaaa tcattctttg taactaatga
    18721 acgaccatct aatcccatac ctgcaatgga taaagtagcc cagataggga atcctgagaa
    18781 taattggtta tattcaggtg tacgcgcgaa tttgaccata cgtaatttca ttactaagtg
    18841 gtcgatcaat tcttgtgctt ctttttctgt gattactcca ttatccaaat cacgttggat
    18901 atagatatca aggaatgctg agatacgtcc gattgacatc gcagcaccat tttgtgattt
    18961 gattgctgct aaatatccga agtatagcca ttggacagct tcttttgcat ttgcagctgg
    19021 tttagaaata tcatagccat atttcgcagc catttctttc atttgcgcta atgcacgata
    19081 ttgttcagtg atttcttcac gtaaacgaat cacttcttca ctcattgtat gataaccagt
    19141 catatcatga tcttttttct tttgttccat caaatagtcg attccataca atgccacacg
    19201 acgatagtca ccgatgatac gtccgcgacc gtaagcgtct ggaagccctg taatgatttt
    19261 gtttttacga gcagcacgca tttcagcagt ataagcgtca aatacacctt gattatgtgt
    19321 tttgcgccag tcagtgaaaa tatgtgtcaa ttcatcgttt ggtgtatagc cattactttt
    19381 tagcgcattg ttagccatat tgattccgcc gaacggcatg aatgcttgtt tcaaaggttt
    19441 gtctgtctgt agaccgacga ttttttccaa atctttatcc aaatagcctg catcgtgtga
    19501 agtaatagtt gatacgattt ctgtgtccat atcatagaca ccgttacgct cgaattggat
    19561 atcatttaac ttttgcaatt catcccataa tttttctgtt gcttctgttg ggccttccaa
    19621 aaagctgtca tcaccgcgat attcagtgta gttattttga ataaagtctc tagtgtctac
    19681 gccttctttc catttggtac ctttaaatcc attccattgc tccattgtat ggacctccta
    19741 aatttctgtt ttatgtaaca gtttttacgt gattagtata acacaggacc ctatctttgc
    19801 aagggttttc ttgtttttta tttaacaatt tttatgtttt atttacatat atttaaatca
    19861 acgttgacat accaacattt aagcattgtg aacactttca tctttttttg caaacgtata
    19921 aggatttatt aaaaatgaat gaaaaagaga cgccgcctgt attaatacag atggcgtctc
    19981 ttcattaaaa tactacaaca gttacgactc gatgaaaatc tcaggagcat cgataacttc
    20041 gatgacttct ccaccttttt tctcatccat cgcaaatgat ccattcgatg ttcgatcacc
    20101 tatagggaaa tcagttgggt caatcgttaa ctgctgccct ttttgattca tcaaggttat
    20161 ttcttttgat cttccttccg acatatagat aatacggtgc ggattttttt tcagctcacg
    20221 catgaccgtc actccccgct tcgcacgtga agtttggctg atttcttgag caagcatgcg
    20281 tttaacagct cctcgctgag taacgattac gatcggcgta tcaccttctg ctaacacaag
    20341 cagcccgttg ataatcctat ctccatcttt caaattcata gacttcactc cagctgcctt
    20401 tgctccaaca acgggaacat ctgttaaagg atagcgcaaa cccattcctt gatgcgttac
    20461 taagaaaaca tctaagcctg gctgatcttc tttcagatac acagcaatca gttcatcttc
    20521 ttccgccttt agcttcatac agctgagcgg acggctctta taggtgcgcc atggttcgaa
    20581 ctctgtcatt cgtgtttgct tgatcaatcc attcttgctg atgaatacaa atgttttctc
    20641 tgcttgaatt ttttgatatg ggaaaacagc taaaatcgat tcgtctacag acagattgga
    20701 gattgtttga gaaatgtgct caccagcatc tttccatttc aaatcgggta gttcatggac
    20761 agggcggtag atcacatttg ctttatttgt gatcagaaga acatggtcca atgtactcaa
    20821 ttcacctgag tatagcaagt agtcgccttc tcgcatcccg atttcttctg gtttggaagc
    20881 actataagaa cgaaggcttg ttcgcttcac atagccttct cgcgtcacac tgacaattac
    20941 gtcttcttgt gccactaaga cttgcgtatc aattttgatt tcttcgattt cgtcttcgat
    21001 tgtcgttaga cgagcggaag aataattttt tttcacttct cgcaattctt ttttcataac
    21061 agagaaaagc tctttgtcat ttgaaaggat tttattcaac tctgtgattt gcgcaatcaa
    21121 gctttcggat tccttttgaa gctctgtgat atccgtgttc gtcaaacggt ataattgcaa
    21181 tgtgacaatt gcttctgctt gttcttctgt aaattgaaag acgtcgacta aattcttttt
    21241 ggcgtctttt ttgtctttac tttcacgaat cgttacgatt acttcatcca gaatcgataa
    21301 agctttcatc aatccttcta cgatatgctg tcttttttga gctttggcta aatcgaattg
    21361 actacgtttt gttatgactt gctttcgatg ctcgatataa cttcttaaaa tgtctttcag
    21421 tccaacttga tgcggagtca tatggtcgat agcgaccata ttgaaattgt aattgatctg
    21481 tagctcagtg tttttgaaca aataattcaa aattccttga gcattggcgt cttttttcaa
    21541 ttcgacgaca atttgcagac ctgttctatc actttcatct cgtacttcag ctattccatc
    21601 gattttctta ttcaaacgga tctcatccat ttttttcacc aaggtcgctt tatttacttc
    21661 atatgggatt tctgtgatca cgatttgctg tttattaccc ttcattgcct ctatctttgt
    21721 tttcgaacga agaataactt ttcctcgccc tgtttcataa gcttttttga tttcatcttt
    21781 tccttgcaag atccctcctg ttggaaagtc aggtccagga atatattcca tcaatttttc
    21841 aaggctcgct tgtggatgat cgatcatata gattgtgcca tcaatcactt cagctaagtt
    21901 atgagtcggg atttctgttg catatccagc agatatacct gtcgatccat tgacgagaag
    21961 gtttgggtat cgagctggaa ggactgttgg ttctttttct gtatcatcaa aattccacac
    22021 gagatcgact gtttcttttt cgatatctgc tagcatctct ccacttaact tggataatct
    22081 ggcttcagta taacgcatcg cagcaggtgg atcaccgtcc atactcccgt tattcccgtg
    22141 catttcgatc agtacttccc gcaacttcca gtcttggctc atgcggacca tcgcttcata
    22201 aatactgatg tcaccatgag gatgataatt ccccatgata tttccgacag attttgctga
    22261 tttacggaat cctttgtcat acgtattgcc atctttgttc atggcaaata atatccggcg
    22321 ttgcactggc ttcaatccat cacggatatc aggcagtgca cgttcttgga taatatattt
    22381 ggaataacgt ccgaagcggt cacccatcac ttcttctaaa gttaattctt gtacttcttg
    22441 tcgattttcc atcaggtgac ctcctattct acttcaaata actgattgtt ttcattttta
    22501 tctgctcgtt cttcatctaa taaatcatta gaaactgaag gagagatttc agtatcctct
    22561 tttttatcta agatactgcc atcttcttcg agcgtaaatt gcacgtgatt ttcgatccat
    22621 tttcttcgag gttctacttt gtcacccatc agtgtggtta ctcgacgttc agcttgcgca
    22681 gcatcatcga tacggacgcg gatcaacgta cgggaagtcg gatccatcgt tgtttcccac
    22741 aattgttccg cattcatctc tccaagtcct ttatatcgct gcagcatata tcctttgccg
    22801 acttttttaa tcattgctgc caattcgtca tctgtccacg catattcaat gacttgcttc
    22861 tttccttgtc ctttggatac tttatataaa ggcggtaacg cgatatatac tttcccagct
    22921 tcaatcagcg gtttcatata acgataaaag aaagtaagca gtaatacttg aatatgggcg
    22981 ccgtctgtat ccgcatcggt catgatgatt actttgtcgt aattacagtc ttcgatagaa
    23041 aactcaggac cgactcctgc accaatcgta tagatcatcg tattgatttc ttcatttttc
    23101 aagatatctt gcattttagc tttttcggta ttgatgactt ttcctcgtaa aggtagaatt
    23161 gcttggaact ttctgtctct tccttgttta gcagatccgc cagcagaatc cccttcgact
    23221 aaatacaatt catttttctt agggtttcgg gattgtgcgg gtgtcaattt tcctgaaaga
    23281 agagattcgc cttttttacg cttcttccca tttcggcttt cttctctggc tttacgtgct
    23341 gcttctctcg cttctctggc tttgattgct ttacggacca acatctggct catttcgcta
    23401 ttttcctgca aataaaagcc catttgttca ctgatgacgt tatctactac ggtacgagca
    23461 actggcgtac caagtttttc ttttgtttgc ccttcaaatt gcagaagatt ttcaggtaca
    23521 cggatagaaa gtacagcagc taacccttct ctgaaatcac taccttccag attcttgtcc
    23581 cgttctttca gcaaaccaac ttttctcgca tattcattat aagatttcgt cattgccgct
    23641 ttcattccag cttcatgggt tcccccatct tttgtccgaa cgttattgac aaaagataag
    23701 acattttctg aatacccgtc attgtattga taagcgacct ctacttcgat accttctttt
    23761 tctcccgaaa aataaacaac tggtgtcaat gtatcttttt cttcattcag ataatcaaca
    23821 aattctttga ttccttcttc gtaatgaaaa atttccttga ctggtacttc tccacgaaga
    23881 tcagaaagag agattttaac gccttttaga agaaaggcag attctcttaa ccgttcggct
    23941 aaaatttcat atgaaaattt cgttgtactg aatattgtat cgtctggtaa aaaatggaca
    24001 gtcgtcccgt ttgctttttt tgtttttcct atctttttca acgtaccgtc aggtttaccg
    24061 ccgtttttaa atttctgctg atactctacg ccatctctaa cgatcgttac agttagccat
    24121 ttcgataagg catttaccac actggctcct acaccgtgca gtccaccaga agttttgtac
    24181 cctccttgac caaactttcc gccagcatgc agcacagtaa aaatcacttc aactgttggt
    24241 attccggaag catgcattcc tacaggcatc ccacgtccgc tatctgcaac agttatacta
    24301 ttgtcttcat ggatggtcac atcaatctca ctgccataac ctgataatgc ttcgtcaact
    24361 gcattatcga cgatttcata gactaaatga tgcaagcctc ggctgtcagt ggatccaatg
    24421 tacatccctg gtctttttct aactgcctct aatccttcca atacttggat ggacgagtca
    24481 ttgtattcgt tgtttacttt ttttgccaag gcaaacgctc cttcaataat actttgcggc
    24541 aaaaccgcac cacgattatc atactagaaa aggtcgtgaa aaaaaagagg gtagacgaga
    24601 aatcaatttt aattatctcc taaccgcttt ttcgacattc ccctctatcg atcatctctt
    24661 ctttgtggtc atttttattt ttggttttct ttcaacttac caagttcaat cttaatacaa
    24721 cgatccatca cgatatctgt acgtcctgct tttcttagca tctcagctgc ttctttgttt
    24781 tcaatcccta attgtgccca gaacacttta gcatcagttt tcaaaaaatc atctgctact
    24841 tcaggtaaaa attcactccg tctaaataca tctacgatat cgattggatc atcgatttct
    24901 gtcaaattcg catagacttt ctcgcctaga atttcttcac cagccaataa tggatttact
    24961 ggtatgattt tatatccatt gtcttgcaat acctgtgcaa ccatgtaact tgttcgttct
    25021 ggtttattgc tcaagcctac gacggcaata ttttttgctt cttccaaata gttgaaaatc
    25081 gtttcttgtg ttggattttt tacagacatt tctgccaccc cttgcttttc tttttattcc
    25141 atctattata cttgtatctt tcattttttc aattgttcct gttacctatt catgctaaaa
    25201 taaaggatag ttttcaactt gaaagagggt aaaaaatgaa attgatctta ttacttattt
    25261 tagcatatct tttaggttct attccttccg gtgtctggat tggcaaattg tttttcaaaa
    25321 aagatatccg acaacacggg agcggtaata ctggaacgac caatacattt cgtgtattag
    25381 gcaaaacagc cgggattgtt gtcctgctta tggatatttt aaaaggtaca ttggctactt
    25441 gtttgccgat gatcttccac ctttctggga ttaaccctct ttggtttggg gtatgcgcca
    25501 ttcttggcca tacatttccg atttttgctg ggttcaaagg aggaaaagct gttgcgacaa
    25561 gtgcaggaat gcttttagga tttaatcctg tattttttgt ttattcttgt atcatcttta
    25621 tcgttagttt attttgtaca agtatggtga gtttgagcag tatgatcagt gcagtattga
    25681 tcactttatc tacgatcgtc cttccttatg tagctcctgt gattttggct aagcccaatt
    25741 ggttactgac gatcattgcc tttttagttt cttcctttat cttttatcgg cataaagaca
    25801 atatcaagcg tatcagaaac catactgaaa gtcgaatccc gtttggtttg aatagcagca
    25861 agaaaaaatc ctgatacttt ttcattccaa agagataaaa cgggagtgtg acaaatcgat
    25921 gtcacactcc cttattactt acaggatagc ttttttacac accttggtaa acggtgttcc
    25981 ctgctaactt ctcggtcaca acctcttact tcactgtgat ggaatatttt gtcttgaaga
    26041 tttcatgggc acctagttga ttgatcccta ctttttccat caagtttcca ctactgttca
    26101 ccgcgtcagc gattccgcac cacggttcga tacaaacgaa aggcgcttct tttggataag
    26161 gagaccagat ccctacataa ggcatatctt tatagcttaa agttacgcta tgggaagatt
    26221 tttcacttcg aatggcatac gcatttagac cccttgtttc aaaaaccaag gcatcttgct
    26281 caaaaatttc acgattcaaa gccagattcg tatttgtctg acctaacgtc ttttgatcca
    26341 tatcgatgaa tgggcctttc aatggtaggc gtaatcttga tttcaatgga gaaaatgcca
    26401 gatagaaatc ttcaaatgac aatccttctt caagaggtac attgaaagca ggatgtccac
    26461 cgatagaaaa gaacatttct ttttcaccgg tatttgccac ttgataatga accgtgatgc
    26521 cgtctcctcc taattcatag ctgatcacta attcaaaatc aaatggatac ttttctaatg
    26581 tctccggcgt actccttaag atgaaactag ccatttcttg tccatgttca atgacttcaa
    26641 aatccatatc ccgagcaaat ccgtgttgcc ccatttgata tgttttccct tgataaacat
    26701 actgatcatc ttttaaacgt ccaacaaaag ggaataaaac cggtgcatgt ctgccccaat
    26761 acgctggatc accttgccag atatactcta aaccatgttc ttttgatttc agactgatca
    26821 attccgcacc gtgttcagcg atttcggcga tcagctggtc attttgaata ataacagtca
    26881 ttgccattcc tccaacttcg acttactttt taaaatggga aggctggaac aaacatttct
    26941 atctgcccca gtccccttac ttctaataac tgtgttttgc taactcgtct ttaaaacggg
    27001 aattcaatac ttttaaataa gttcccttca tgcctaacga acgagattca atgattccag
    27061 ctgattccaa ttttcgtaaa gcattgacga tcacagaacg agtgatgccg atactatcgg
    27121 caatattaga agcagtcaga cgaccttcat ctccatccaa tgcttcaaag atagcttgta
    27181 cagccttcaa ttcgctataa gataatgtgt tgattgccat ttggacagcc gttgcactcc
    27241 gtacatctgc ttcgatgctt cgagacttct gataaaggat ctgcataccg acaaccgtag
    27301 ctccatattc tgctaagact agatcgtcat ctgcaaacga ttgttccata cgagataaga
    27361 taatcgtacc caaacgttcg ccagctccaa agatcggaac gacagtcgtc agaccaaatg
    27421 gatatttctc tcttgactct accggaaatg cagtaatatc actagtaatt gggatattag
    27481 cttcagtttt catcaactgt gccactgtat cagtataaga ttcaggaaat tgtttctcaa
    27541 caaacattgt tttgattcgt tcattgttca catcgtgacg ttcattataa cctagtaata
    27601 ctccgtcact tcctatgata taggtgttgc tgtcaagaat accacctagt gtgacagcca
    27661 ttttatcgta aggcaaatct gcttgcatgt caaaagtatt cttttgctgc aaaagtgtgt
    27721 ttatcttgcg ggttttcgtt agcaagtctg tcattgtcgt cctccgattt tttataaaat
    27781 atagcgactt aaatcttcat ttgccgcaat tgagccaagt ttgtcatcca catatgcttc
    27841 tgtgatcgtt atttctccca tttgcatatc tggtgcttca aataatagat cttctagtag
    27901 tttttccaaa atggtatgca gtctgcgtgc gccgatatta tctgtgtcac ggttgacatc
    27961 gaaagcgatt tgagcgatcc gttcaattgc ttcttttgta aacgtaactg acacattttc
    28021 tgtaccaatc aatgctacat attgttttat taaagcatta ttcggttcag tcagaatttt
    28081 cacaaaatct tcagctgtca aatcatctaa ttctacccgt attgggaaac gcccttggag
    28141 ttctggaatc aaatcactcg gtttagataa atggaacgct cctgaagcaa tgaataagac
    28201 atgatctgtt tggatgatcc cgtattttgt atttacttgt gaaccttcga cgattggcaa
    28261 tatgtctctt tggacgcctt ctctggatac ttctccagat tgctgtgatt tagaagtgat
    28321 cttatcgatt tcatcgataa aaataattcc actttcttcc gctaatttga ttgcctcact
    28381 atggatatca gcatccttga ctaattttgc tgattcttcc ttgatcaata actctaaagc
    28441 ttctttgact gttaccgtcc gttccacttt tttcttcggt gtcaaagcac ctagagtttc
    28501 atttaggtcg atccccattt gctccaatcc attgttcatc attggtgcag gtttcttttg
    28561 gtcttcaact tcaatggtta cttcacgact atccagtaaa cccttttgta gttgttccaa
    28621 gactgtacgg cgattgattc gaatatcttc tgtcacttct tcttgcggtt cagcggtatt
    28681 ttgctgacta gcattaaaaa gattcatcat ttgttcaaat tgattagtgg attgtttctg
    28741 ttcttttttg ataccaggta ccaagatttt taccaaacgt ttttctgcat tttttttagc
    28801 acttgtgtat acacggctgt actgctcttt ttctacgatt tggatcgcat tttctactaa
    28861 atcccgaacc attgattcta catctcttcc gacataaccg acttcagtaa attttgttgc
    28921 ttcgactttg acaaatggtg cattgacgat ttttgccaaa cgtcgtgcga tttctgtttt
    28981 accgacccct gtaggtccga tcatcagcat attttttggt gtaatatctt gttgcatttt
    29041 ttcatctaac tgcaaacggc gatagcggtt acgcaaagca acggcaacag atttttttgc
    29101 tgcgtgctgt cctacgatat attcatctaa ttcacttacg atttgtcttg gtgtttgttt
    29161 taattcatac atatctactc acctcttaca gttcttctac gataatatta tgattagtaa
    29221 agatacagat atctgcggca atatttaacg cattttctgc aatttctttc gcactcatgt
    29281 cgttttgagc atagtttttc attgctcggg ctgcagccaa cgcaaaattc cctcctgatc
    29341 cgatagctaa gataccatca tctggtgtga taacttctcc tgtacctgaa acaagaagca
    29401 tttctttatc attcattacg atcagcatcg cttcaagttt ttgcatagat tgctgtgtcc
    29461 gccattcttg agctaattcg acagctgcgc gttgtagatt cccgttatat tcgtttagtt
    29521 tgccttcaaa tttttcttct aaagtaaacg catctgcaac acttccggcg aaacctacaa
    29581 caacttctcc attgtagata cgtcgtactt tctttgcagt gcctttcatg acaacagatt
    29641 cacccattgt tacttggcca tctccagcca tagcaaattt cccgtctttt tcgacggcac
    29701 agatagtggt tgaatgaaat ggtgattcga ccatgtttgt ttcctcctaa atttgttctt
    29761 ttccttattt ttacgctctt ggatggaaag aacgatagtt tttttgtaaa ctttcttttg
    29821 ttacatgggc atagatctgg gtagtcgata aattggcatg tcctagcaat tcttggactg
    29881 tccgcaaatc tgccccatta tttaataaat gagtagcaaa tgtatggcgc agcatgtgag
    29941 gatggatatc actatccaaa ctgctttttt tgatcatttg attcaagaca tattcaattc
    30001 cagtaggcgt gattggtttt ccatgatgat tcacaaaaac gatatcatgc tcttcgtgat
    30061 attttgtcat caatacctgg cggccttccc tgaaatattc ttgcagagca tcttgagcaa
    30121 aagaacctag aggagcatat ctctctttgt tccctttacc atggatcaac atgacctctg
    30181 aatcaaaatc aatgtctgat accgacaaac tgctacactc acttaaacgg atgcctgacc
    30241 cgtaaaggac ttccagtaaa gcgcggttcc tcaaatccaa aggactatct cctgctacgc
    30301 tgtcaaaaag tgcttgcatt tcattttcat aaaaaaaacg tggcagtctc aaatttttct
    30361 ttttgagatg aacgtaagaa aatggatttt cttttatcac ttcttctttg agaagatact
    30421 gataaaacga acgtaggctg gctatcttgc gactgataga atttctgcta tagctaggac
    30481 gatgatcagc taatgcactc aaatagacac gaaccgtaaa atattccgcc ttcagcagat
    30541 cagcgtctcc agattcctcc aaaaaatgtt tgaaatggag aatgtcttct tcatatgctt
    30601 ctttcgtttt ttctgaatat cctctttcga caatcaaata acgcaaaaat ttttgttccc
    30661 acgtggcttc tgtcattttc tcaccttcct tttgtcccag agaataattt agcacagcgt
    30721 ttataaaaag acaaataaat tgtcagaatt taaccaatat ttctaagttt gttcacaatt
    30781 ggcgcaattc ctcacatttt gtttgagaaa ttcttaagaa aaacgcaaga aaaagagcga
    30841 gactatccgt ctcaccctct tcatggagtt tccttactca tttgggattg cagttttttc
    30901 taattctgtt tttattgcct cattagcttt taaagctcgt tgtgcaattg cttcatatct
    30961 tgcttttttg tctcgaatgc gctctggcag ttcaggaaac agaccatagt tggcattcat
    31021 cggttggaaa tgttttcctt ccgcatgagt aatgtagtat gccatacttc caatagctgt
    31081 ttcttgtgga aactcgactg gttccatttc ttttgctaag cgtgcggcgt tgattccagc
    31141 aagtagtccg ctcgctgcac tttcaacata gccttctaca ccagtcatct gacctgcaaa
    31201 gaacagatct tcgcgtttcg ttgattggta agtcggtttc agcaattctg gcgaattcat
    31261 aaagctgttt cggtgcatca caccatagcg aacgaattca gcattttcta acccaggaat
    31321 catccggaaa acacgttttt gctcgcccca tttcaagtga gtctgaaagc ccacgatgtt
    31381 gtataaagaa gctgcagcat tgtcttgacg cagctggata acagcataag gacgtttgcc
    31441 agttttagga tcttccaatc caactggttt cataggtccg aaaagcatcg ttttgaatcc
    31501 tcgctgtgcc attacttcaa ttggcataca tccttcaaaa tatttttctt tctcaaaaga
    31561 tttaagttct gccacttcgg cattgattag tgcctcatgg aaagctttga attcctcttc
    31621 tgtcatcgga cagttcaagt aagctgcttc tcctttgtca tagcgggact tcaaatatac
    31681 tttatccata tcgatcgtag ctttgtctac gattggtgca gctgcatcat agaaataaaa
    31741 accatctgaa ccattgaatg ctttgatgct ttctgctaat ggatgagaag tcaaaggtcc
    31801 tgttgctact acggtgattc cttccggtat ttcagtaatt tcttcgttta tcactgttac
    31861 taatggatgc tctttgactt ttcgggtaat tgtttcagaa aaagaatcgc ggtcaacagc
    31921 taaggcacca ccagctggca ctgctgtttc atctgccgaa gtgattacta ccgaatccaa
    31981 tcggcgcatt tcttctttca ataaccctac tgcattagcc aaactgtttc ctcttaatga
    32041 attggaacat actagttcag caaaattact tgtgtgatgc gcctcagtcg atttgactgg
    32101 acgcatttca tataaacgga cagggactcc tgcttgtgcg atttgccaag cagcttcact
    32161 tccggcaagc cctgctccaa caacatttac cattttcaat gaaagaacct cttttctatt
    32221 atttttggat gtcttcttcg taatctccat ttacacagac tacttgttta ccgcctttga
    32281 ctttcttctc gaccagatat ccgtcacatt ttggacaatt tctgcccact ggtttatccc
    32341 aagaagtgaa ttcacattct gggaaacggg aacaaccata gaatagtctg tttttcttag
    32401 attttcgttc aatgacttgt ccttcatgac atactgggca agtcacaccg atttctttta
    32461 cgattggttt cgtattccga caatcaggaa aattactgca agcataaaac ttcccatatt
    32521 tccctaattt tattaccatc ggatgtccac agacatcaca atcgaatcct gctggttcat
    32581 ctttgatttg aattttttcg attttttctt ccgcattcgt cagttctttt tcaaacggtt
    32641 tatagaaacg atcaaccact ttgacccacg cttccgtgcc ttcttcaatt ttatccaaat
    32701 ctccttccat ttctgctgtg aaatggatat cgacgatttg agggaagaat tcacagatca
    32761 gtgaattgac aatttctcct agttcagtcg gctcaaatct tttttgtgtc aattttacat
    32821 agtagcgtct ttggatcgta tcaagtgttg gtgcataagt ggaaggtcgc cctaccccgt
    32881 tttcttctag tgcacgaatc aacgtagcct cactaaaacg tgctggtggt tgagtaaaat
    32941 gttgttttgg ttcaatatca acagattgga ctatatcgcc ttcttcaagt tctggaagaa
    33001 tattttcttt ctcttctttg ccgtcatctc taccttccac atacacttgc atgaatcctt
    33061 tgaatttgat ttttgaaccg ttcgcaatga agatgacacc attttgttct aatgtcactt
    33121 tcattgtatc taaaatagct ggtgtcatct gactagccac aaaacgtgac cagatcaatg
    33181 tataaagttt caattgatct ttatctaaat attgtttgag ttcatctggt gtacgaagca
    33241 cactcgttgg acgcacagct tcatgggcat cttgtgcgcc ttgagcattt ttgatctttc
    33301 ggtgatcatg tctagaaaat tcttttccat acgtatcttc aataaatgct gcggcctctg
    33361 cttttgctgt atcggcaatc cgggtagaat cagtacgcat gtatgtaatc aagccgactg
    33421 ttccttgttt acccaaagtg attccttcat ataattgctg tgctaccatc atggtttttc
    33481 ttgttcgaaa gtttaactta cgggcagctt cttgttgcag gctacttgta gtgaaaggat
    33541 tcgcagggtt tcttttgcgc tctttctttt ctactttttt aacttcgaag tctgttccat
    33601 caattctagc agtgacttct tttacacttt gtgcatctgg taatttcttt ttcttaccgt
    33661 cgattcccca gaaatttgct ttgaatttct tgcgagcttt tttaaaatta ccgtcgatgc
    33721 tccaatattc ttctggaaca aattctcgga tctctttttc acggtcaata ataattttta
    33781 aagcgacaga ttggacacgt cctgcactta accctttctt gaccttacgc cagagaatag
    33841 gactgatcga ataaccaacg attctgtcta aggtacgacg agcttgctgt gcatctacta
    33901 aatctacatc gatcgttctt ggttccttaa aagctgcttt gactgcgtct tttgtgattt
    33961 cattgaagac cacccgattt ttttctttca gatccaaccc aagtaggtac gctaaatgcc
    34021 aagcaatcgc ttctccttct ctatccgggt cacttgccaa gtaaactttt tgagcttttt
    34081 ttgctgcggc tttaaggctt ttgatgacat cgccttttcc gcgtatagaa atataatggg
    34141 gttcataatt gttttcaaaa tcgataccca ttttactttt cggtaaatct cttatatggc
    34201 caacactggc cattacttta tagtttcgtc caagatattt ctcaattgtc ttggcttttg
    34261 caggtgattc tacaatcact aaatatttat acgccattac tgaatggact cctttccatt
    34321 gtcgtgttcc tactattaaa agaggattca tacaaatcgt agctaatcat atccattcag
    34381 aaaacgtttg tcaagaaaag caaatgaaaa gtatggtgtt attctttaaa ttctgccaag
    34441 atatcttcac ccgaaaccgt gcaaactgca ccgtcttgga tcaaatgatg acatccggta
    34501 gaatgttctg ttaaaatatt tcctggaaca gaaaaaactg ttcttccaag atccaatgct
    34561 tgctgtgctg ttatcagaga gccgcttttt ttcttggctt caaagacaca tgttccttta
    34621 gacaaagctg cgataatccg atttctcatt ggaaaacgat gctttttcgg gccggattga
    34681 ggaggatatt cactcaaaag aagatgatca tttgcaatca cctcttgcaa gtcacgattt
    34741 tcagcaggat agacttgctc tatccctgtt ccaatgactg cgattgtccg tccaccatga
    34801 gagattgctg cttgttgacc gtatgtatct atccctttcg cgcagccact gacgatcaca
    34861 tatccagctt ctaccactgg ttttagtaaa taataaactg tttttttggc taaagcagaa
    34921 gtgtgtcttc caccaacaac gcttaacatg ttacaagaaa gtaaagatag atcaccatta
    34981 taaaacagta caattggcgg ataggtcaag tgaagtagct gagggggata tttgggatca
    35041 aagcaagtga taaacgactg tttcgattgg attttgcccc agttagcgtg aatttcctgc
    35101 caacttttgc gaaacagctc ccgatgtgtg gttattttac ctatcttaat gatgtcttca
    35161 aatccgattt ctttataagg atactgcatc atccagttag caagtgccca ttttccttga
    35221 ctactgattc cttttgcata agcaagtttc aataaattct cttctatttg atacatcata
    35281 aaaccgcctt tcgtctctct ttctaataag atacgtaaaa gcggctgttt tttattgtaa
    35341 atattttttt acaggtgcaa aacttttacg atggattggc gtaattcctt gtcgttcaag
    35401 gccttctaag tgttcctttg ttccataacc tgcattatgc gagaaaccat atccaggata
    35461 aagcacgtca tattctttca tcaattggtc tcgatatact ttagcaatga tacttgcagc
    35521 agcaatcgac acagatcgtg catccccttt gattattttt tcctgctgga taggcaaatc
    35581 caaggtcatc gcatcgatca acaaatagct tggtacaatg gacaattttt ctactgcttg
    35641 gatcatcgct aatttacttg cttcataaat attcacatca tcgattgtct gctgatcaac
    35701 gatcccgatc ccgattgcca gggctttttc ttgaacttgt tgataaagaa gttctcgctt
    35761 tttctctgat aattgtttgg agtcattcag ccctaatatg atctcatttt tcggcaagat
    35821 aactgcagct gccacgacag gtccagctaa tggtcctctt cctacttcat caatgccggc
    35881 aatccattgg tgaccttgtg cgtacccttg cttttcaaag caaaacattt cttcatattt
    35941 ttccttaatt aatgcttcct ttttcaatcg tcgttcaaaa gaagccagtg cttgctgcac
    36001 gcctttgcgt ggatcatttc ttaatgattc tagtgtagga tcatctattc tctgtatgct
    36061 tttcaatcgt tctttgatac tttggatgga ttcagtcatc acttttttgc tccatttctt
    36121 cgcaacgatc cagtgtgtat cgtccaagtt ttccttggcg aatatcatgc acgactaatt
    36181 cgctgccgcg atcatagtct tctttatacc cacgttttgc tgtaatatcc atcagtaatt
    36241 ccggtgccaa tttttcttct tgctcgattg ataaaccata acgatcgctg attcttcctg
    36301 aataatatcg ggcaaaaaag tctaatccgt aaatcgctaa atcatctaaa tggacaagct
    36361 ggtctttgat cgcgcctgtc agcgctagtt ttttgccgat ttcttcatct tcaaatttgg
    36421 gccataaaat ccctggtgtg tctaacaatt ccaactcatt acctaaacga agccactgct
    36481 gaccttttgt tacgccaggc ttatttcctg tctgtgcaat tttcttgcct actagtcgat
    36541 tcatcaacgt agatttccca acatttggaa tacctaagac cattgcccga atagcgcgag
    36601 gcttgatccc ccgagcagca tctcttgccc gtttttcttt caatgcttct ttagccgcag
    36661 gtactagacc tttaattcct ttactttctt gtgccgtgat agctaatgca agatggcctt
    36721 gtttacgaaa atattcttgc cacagctttg tctgttgtgg atctgccaga tccgctttat
    36781 tcaataaaac aaggcgaggt ttttgctgaa tgatctgatc tagcattgga ttacgagaag
    36841 ataaaggtaa tcgtgcgtct accaattcaa atacgatgtc cacatatttc attttttcag
    36901 aaacttctcg tcgggctttt gccatatgtc ccggaaacca ttggatggtc attgttctct
    36961 ccattccttt cataccggaa tattccccgg ctttacttgt tttttttctt tttcaatata
    37021 aattcacttt tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc
    37081 tcattctatc acatcttcac ttttttgatg atgtaaattt ttattttggt ttctatatat
    37141 gaatctaaat ttttgacaac gtttcctaaa taagagatga tagacgaaca aaacagaaag
    37201 gagacgaaac aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt
    37261 tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa
    37321 gctggttcaa tggtatggga tgacactttt tgggtaatgc ttctctctca tgatttttat
    37381 aaaaaattta taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt
    37441 tcgtttagtg ttgaaaacgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat
    37501 ggtggtactt tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa
    37561 gtccaagatc tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac
    37621 taatctgacg ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc
    37681 ctctattttt ttcattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc
    37741 ctttttttaa ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt
    37801 gattttatgg tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga
    37861 ttgcaaaaga cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc
    37921 ttttattagc ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa
    37981 tcacattcgg atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg
    38041 gtttgcttga aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc
    38101 aagctgcttt tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc
    38161 ataaaaagat aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca
    38221 gcctttttat caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtgattt
    38281 ttctaggtag agtcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt
    38341 catttatcaa aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac
    38401 taaggtagta aactgatttt ggattaaaca ataaagcgtg cagtctcaac agattgcacg
    38461 ctttattgtt tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc
    38521 tttacctaga atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct
    38581 tcgattatct ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc
    38641 ttgtagtgta aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt
    38701 tagataaggt tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac
    38761 attttctcct ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa
    38821 aacaacaata tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg
    38881 cttcaacacg ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg
    38941 caacatgaac gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt
    39001 aatatagcgt tgtttttttg tcaatctcta tcaatccatc cctcgttttt tcattctttc
    39061 tttattataa gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt
    39121 tcaattaggc tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata
    39181 tgtcatgatt attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca
    39241 aatagtcgac attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag
    39301 gaaattggat gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac
    39361 cgcctcgcag tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt
    39421 ttcttcgata aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt
    39481 ataatactct tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt
    39541 aacgattggc tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat
    39601 gatccgctgt tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc
    39661 gaattgtccg aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc
    39721 atatgaaatc aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg
    39781 gctttttact tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac
    39841 tgtatggata ccaggaagcg tgtcgaaagg ctggatcata caccagaaac agcctcctgc
    39901 aaatattgct cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat
    39961 tttcttttgt cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg
    40021 aagcatcaga ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt
    40081 cgattttatc tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca
    40141 tgatagtcag atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga
    40201 tcatattcat cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt
    40261 ttagttggac atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag
    40321 gaaaacctag tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat
    40381 attcaatatc actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt
    40441 tttctttatt tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta
    40501 tttctgggag ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac
    40561 caagtactac cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt
    40621 tgtttgaaga tacgtcagat cttgttttca tgtttattga tcatcctcct tctctagcca
    40681 ttggttttgt gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa
    40741 attatttgga tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt
    40801 ttctgtcgtg ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa
    40861 tccttgataa agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac
    40921 cgtttctgtt cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa
    40981 gttcaaataa aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga
    41041 tcgaaattct tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa
    41101 ggaattgacc gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc
    41161 aacagtcagt gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt
    41221 tactcgcttt aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac
    41281 cgcggtcaaa ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg
    41341 cttggtttga tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt
    41401 ttctttttta tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt
    41461 cggtatagaa gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac
    41521 gaggatcaga tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta
    41581 atgttatcaa aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc
    41641 ctaattttat aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc
    41701 ttttcgacta atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga
    41761 taaccggatt cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct
    41821 ctttgaattc ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat
    41881 ttggcaattc tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc
    41941 ctcgcccctt agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt
    42001 tcaaaacact agacagcaaa ccagttgttc gactgattct tccacctttt accaagttat
    42061 ctagtgtgga tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa
    42121 ggatttcctc tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag
    42181 ataatccttg gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac
    42241 tggcttgtct tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca
    42301 cttcactgcc gtcttctcct agctcatcat acatcgagat aaattctcca atcggtggtt
    42361 ggcttgtttt gggcagattt tttgcttttg ccatcatttc cataaatctt tcgccgtcta
    42421 gttggtcatc atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa
    42481 tgcctaaagc atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta
    42541 catttgtcat tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta
    42601 catgtaagac aatttctatt tttagtataa cagacaaata atgatttttc attaattaca
    42661 cctagtaaag gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta
    42721 aatgaagtgt ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta
    42781 gtggttcttt tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct
    42841 atctatggat cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt
    42901 tttacaaaag caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt
    42961 gcaggaagtt atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta
    43021 tttgtattaa tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat
    43081 aaacgggaga gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt
    43141 gtcgtggctg ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta
    43201 gcaggcgggc tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat
    43261 atgcatgttg tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt
    43321 ttattcttta cttaataaaa aaaaataaag ctatggatgg gacagctcta aataacagct
    43381 agtctatcca tagctttttt atatcgtcaa ttcgcctgtc ccgcttttta caggattctc
    43441 attacgctga tacgtttcaa attgatgcgg ataacgattt ttttcatcta tttctccttg
    43501 actagtattg atcagtgacc aatcagacca gtcaacaggt ggaaaataag catctccatc
    43561 gaatacatga tggatgactg tacggtataa tatcgtacaa aatggcagaa attcttgata
    43621 aacagctgac cctccagcga tgaaaaagat accgtctgtt tctttttcca gagcaagcac
    43681 gtcttccaca ctatgcatga catgcacgcc ttctgcttta taggaagaat ctctcgtcaa
    43741 aacaatcgtc tgacgattcg gcaacggacg gctacccatc ccttcgaaag tttttcgtcc
    43801 catcactagt gtatttgctt ctgtcatttg tttgaagaat ttcagatcat ttggcaaacg
    43861 ccaaggaaga cggtcttctt ttccaatcaa tccgttttca tcctgtgccc aaattgcagc
    43921 taacattttc tcatcctttc tgattctaaa cagcgatagg agctttgatc gccggatgtg
    43981 ggtcatatcc ttctaattgg atatcttcca tctcaaaatc aaatactgac tgcttttcct
    44041 gattcaaaac taaagtcgga aatgcgcgta tttctcggct caattgttct ttcatttgtt
    44101 cgatatgatt gatataaaga tgagcgtctc ctaatgtgtg aacaaattcg ccaaccgcta
    44161 aacctgtttc atgtgcaatt aggtgagtca gcaacgcata acttgcaatg ttgaacggaa
    44221 cgcctagaaa cacatcccca ctgcgttgat acaattggca gcttagccgt ccctcttgaa
    44281 cataaaattg aaacatcgta tgacaaggag gcaaagccat gctaggaaca tcttccggat
    44341 tccatgcaga aacgatcaat cttctagaat caggagtctt tttgattgct tcgatcactt
    44401 ctttgatttg atcgataaag ccaccgtctt ttgtttccca gtgacgccat tgatagccgt
    44461 agatgtgtcc aagatcgccg tatgtttcag caaaggaatc atcggttagg atcttctcgc
    44521 aaaaaagctt gctctgcttt ttatattctt cattgaattc aggatctgtc aaacatctat
    44581 ggccaaaatc agtcatatct ggtccattat agtctggact tttgatgtag cgttcaaatg
    44641 cccattcatc ccaaatatgg tttttatttt gtaatagata gcggatattc gtatctcctt
    44701 ttaaaaacca aagcaattcg cttttgatca atccaaaagg tactcgtttc gttgttaaca
    44761 aaggaaaccc tttttgcaga tcaaaccgca tttggtatcc gaataagctt aatgtgcctg
    44821 tgcctgttct gtcttcttta cgatgccctt cttccaacaa ttttcttcct aagtcaagat
    44881 acgcttgttc catccttttt cctcctaatc ttctacccat tcactcaagt attcccaccg
    44941 tgccattttt tcttcaagtt gtgcttctgt ttcagatacg tcgttttgca attcttgtaa
    45001 tcgagtgaaa ttatctcctt gatgattcat ctcttcttgg agcaatgaga ttttttcttc
    45061 aagttctgca atctcatctt caatcgtttc ccattctttt tgttccatgt aagataattt
    45121 cttcttttca gcaggttcct tgctctgtat ttttggtttc gctttttcag caggctgctg
    45181 ggtctctttt tgcttcagta aataactgga catgtttccg taataaagtt cttgttttcc
    45241 gtttccttgg aatactaata acttatctgc taccttgtcc aagaaataac ggtcatggga
    45301 aacagtgatc acagcacctg gaaaacttct aaaataatct tccaaaatcg tcaatgttgc
    45361 tatgtccaaa tcattggtcg gttcatccaa taataaaaca ttaggttgct ggatcagcag
    45421 tttcaacaaa aataaccggc gtttttctcc gcctgaaagt ttgcctatca gtgtcccatg
    45481 cataaatctt ggaaataaaa atcgttcaag taattctgct actccgattt gactgccatc
    45541 tgctgttttt gcttcttcag cagcttcttg taagtaagca atcatccgtt tatctggtgc
    45601 catttcttca ttttcttgtg tataatacgc aagatgaacc gtttcaccga tagacattgt
    45661 accgctatcc aggggaattc tgccagctag aatattaagc aatgtcgatt ttcccgcacc
    45721 atttttacca gtaataccta gtctttcccg agattgaatc agtaaatcta aatgttctag
    45781 cagtgtttgg tcatttatac ggtaactgcc atctttgatt tccagtactt ttttccctaa
    45841 acgttgtgtc gctaggttca gctctaaatc atcttcttgg tttacttggt acaagttctc
    45901 ctttagatct tcgaaacggt tgatccgtgc ttgctgcttg gtgccacgtg cctgtacgcc
    45961 agccctcatc caagctaatt cctgtttata aagccgcttg cgcttttctt cttgttctac
    46021 ttctacacga tcacgttctg ctttttctaa gacgtaagct tcataatttc ctttatactc
    46081 gtataatttt ccaaatgaaa gttcgaagat tcggtttgct actctatcta gaaaataacg
    46141 atcgtgggta accatcaata gtgcgccacg gtattgtttc aaatagtttt ccaaccactc
    46201 gatcgcttca taatctaaat ggtttgttgg ttcgtctagt aatagtaaat cgggttcatc
    46261 aatcaacact tgcgccagac taactcgttt tttctgacca cctgataaag tactgatttt
    46321 tttatctaat tcactgattc ctaatttttg caaaataatc tttgcgttgg tatctgtagt
    46381 ccaagcatct tctttattca tttgttcttc tgctttagca taacgttttt gcacatcagg
    46441 gtcttctccg ttttccccta gttcgatcaa tgcttcctca tatgctcgaa cagtttggat
    46501 caaggggctg tttccttgaa agactgcttg gagaacagtt aattcatcgg aaaattcagc
    46561 tgcttgagaa agataaccaa tacggtaatc agttggataa aacactgttt gccgatcacc
    46621 gtcgccgcta tcgataccgg caataatatt taagagactt gttttacccg tcccgttcgt
    46681 cccaatcaac ccgatacggt ctttttcgtg gaccaaaaaa gaaatctggt caaacaaatc
    46741 tttttcacca taagtttttt tgaggtcagt tacttttaat tctttcaaca tgttccctcc
    46801 ttatcttgga tattgtatca taaaaatagt gaacttcagg aaaatttttt tggtatttgt
    46861 tctttttgtg ttctcaaaaa aagaacagga ccgattcgat cctgttctga tatgttttgt
    46921 tttttataaa acttgaaaat ttttaccttt ttattttatt ggactcgttt ttttgacttt
    46981 gctcgaaacg caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct
    47041 aacaaattga tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ctcagcaact
    47101 tcgtattcat caatgatttt tctttcaaga taataacctc ttagcatttt ttttgtaaac
    47161 agactatctt gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct
    47221 tcaagtttgc gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag
    47281 ttatctaaac tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg
    47341 atatcttcat cactaataaa tgtctgacga tcagcatgtc ttctttgcca aaacattttc
    47401 ggatggagaa cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac
    47461 aaaatctggc ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa
    47521 ccattagaag aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac
    47581 aaaatcagtg cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag
    47641 tacagatcac gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct
    47701 tttgtattac gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa
    47761 atcatcaaag cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag
    47821 acaatgatcc ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa
    47881 gaccgctggt aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa
    47941 ctgactgttc cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg
    48001 aactttttac tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca
    48061 aaaagcatga cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg
    48121 tctccccata ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat
    48181 gcattcagtg taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca
    48241 tcaaacacag agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca
    48301 atgattccag atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt
    48361 tcgatcagca gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt
    48421 ttttgtttga accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact
    48481 agactcatcc ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac
    48541 tggaaggcag taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc
    48601 gcctttttag ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga
    48661 cctaacgctg ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat
    48721 aacgtcgcac ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt
    48781 gtcccgaaat ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat
    48841 ggtgcaataa tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct
    48901 gtcagaccta ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa
    48961 atgcggctag ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca
    49021 aattccatcg tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct
    49081 tttttcttta aaattttggt ttcgactact tcaacctgtg tttggttacc ctctaattca
    49141 cactgtacac gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga
    49201 tacatcaata tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa
    49261 agggcgccat agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc
    49321 cagatctcct atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat
    49381 ttcattgttt catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt
    49441 ctattccaaa tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa
    49501 agaatgttta cttttccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg
    49561 ctgcgacaaa aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc
    49621 tatcagtagt aataagacac atctctttca cagcttcatt tttgcgtaaa aatcgattta
    49681 ttcgaaaaat tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa
    49741 gttacctgtt ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc
    49801 tttatctaga tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat
    49861 tcgctcgata tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag
    49921 gttgatatca tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc
    49981 acacggatgg atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg
    50041 tttgatccaa agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa
    50101 ataaaatttt agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt
    50161 attcttgtac tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt
    50221 cttctaatga cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag
    50281 tgttttttaa atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt
    50341 tattctttgt tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc
    50401 aggttttttt ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga
    50461 cttgataggt aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa
    50521 atagatcatc aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa
    50581 tggttccaaa gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt
    50641 aatcatttgg ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag
    50701 acccagttgg ctgatcaata atgtccatac ttggctctct tctttcaatg ggacggatgg
    50761 taattcagca aaacgtaaaa gtgcttcgcc ttcttctttc aaactgggac agcaaatgta
    50821 gcactctgtt tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt
    50881 gatgaattcc acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt
    50941 gatggcgtag aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc
    51001 gcgcatcatt cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa
    51061 gatctgttct tctaaatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt
    51121 caaagcaaac gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac
    51181 gaattcgaca tgatctggtc tgcggtagtc ctgataagtt gattccgtcc gaaatgtggt
    51241 gatctcatat tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc
    51301 tatcgttttt ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac
    51361 atcatggatt ggctggttca gcaagatatc acgtacactt ccacctacaa aataagcttc
    51421 atatcctgca ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa
    51481 ttgttttaat ttcatttttc caacctttcc tgcgtgtttc ttgtactgag ataaagactc
    51541 agttgcatgg tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca
    51601 tcttgtttac tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat
    51661 gtttttttca aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac
    51721 gaataaaaca tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct
    51781 tttttctcca taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg
    51841 agccatctga gctaatggag agaaataccc caccttgaat tgctggatat attcatccac
    51901 ttcctgctgc atactgtata aagaacgctt tttatccatc tttttccctc caaaactatc
    51961 ttaccaaaac tttaacgcaa atcctagata agagcagttg agaaaatcga atttccatgc
    52021 tacatttaga aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga
    52081 attcatatgg tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt
    52141 atttatgcat ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggcgtc
    52201 tcagggatca ccttgatctt aagagcttta ttcggaattg atccagctta tacgacttta
    52261 ttaatcaaca tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc
    52321 tatacagttc taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca
    52381 ctagaaatca atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca
    52441 ggtgtaggaa gcgggatcgt ctaccgcatg ggtggaacaa caggtggcag cgatattatt
    52501 gctcggattt tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat
    52561 atcatcgtct tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg
    52621 attgtttcgt atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca
    52681 aaaggtctac ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt
    52741 ctgcaaagag gtgtgacttt tctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa
    52801 atcatctata tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag
    52861 attgacgaaa aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt
    52921 acttatttga agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag
    52981 gctatgagga aa
//
LOCUS       DAFCNS010000010        51280 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFCNS010000010 DAFCNS010000000
VERSION     DAFCNS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.010"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            290..1135
                     /locus_tag="LNE23_000581"
     CDS             290..1135
                     /locus_tag="LNE23_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6930458.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="LNE23_000582"
     CDS             1416..2636
                     /locus_tag="LNE23_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBK6930459.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="LNE23_000583"
     CDS             2633..5353
                     /locus_tag="LNE23_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6930460.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="LNE23_000584"
     CDS             5337..6281
                     /locus_tag="LNE23_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6930461.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="LNE23_000585"
     CDS             complement(6350..7360)
                     /locus_tag="LNE23_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6930462.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="LNE23_000586"
     CDS             complement(7540..7884)
                     /locus_tag="LNE23_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK6930463.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="LNE23_000587"
     CDS             complement(7877..8311)
                     /locus_tag="LNE23_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBK6930464.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="LNE23_000588"
     CDS             8449..9186
                     /locus_tag="LNE23_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6930465.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="LNE23_000589"
     CDS             9179..10390
                     /locus_tag="LNE23_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6930466.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="LNE23_000590"
     CDS             10594..11376
                     /locus_tag="LNE23_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBK6930467.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="LNE23_000591"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="LNE23_000591"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBK6930468.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="LNE23_000592"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="LNE23_000592"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBK6930469.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="tadA"
                     /locus_tag="LNE23_000593"
     CDS             14092..15021
                     /gene="tadA"
                     /locus_tag="LNE23_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBK6930470.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="LNE23_000594"
     CDS             14978..16027
                     /locus_tag="LNE23_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBK6930471.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="LNE23_000595"
     CDS             16024..16302
                     /locus_tag="LNE23_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBK6930472.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="LNE23_000596"
     CDS             16277..16747
                     /locus_tag="LNE23_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBK6930473.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="LNE23_000597"
     CDS             16764..17066
                     /locus_tag="LNE23_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930474.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="LNE23_000598"
     CDS             17083..17454
                     /locus_tag="LNE23_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBK6930475.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="LNE23_000599"
     CDS             17426..17785
                     /locus_tag="LNE23_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930476.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="LNE23_000600"
     CDS             17957..18964
                     /locus_tag="LNE23_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6930477.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="LNE23_000601"
     CDS             18989..20173
                     /locus_tag="LNE23_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBK6930478.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="LNE23_000602"
     CDS             complement(20610..22193)
                     /locus_tag="LNE23_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6930479.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="LNE23_000603"
     CDS             complement(22771..22914)
                     /locus_tag="LNE23_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBK6930480.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="LNE23_000604"
     CDS             complement(23137..23616)
                     /locus_tag="LNE23_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6930481.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="LNE23_000605"
     CDS             complement(23689..23973)
                     /locus_tag="LNE23_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930482.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="LNE23_000606"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LNE23_000606"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="LNE23_000607"
     CDS             complement(24317..25603)
                     /locus_tag="LNE23_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBK6930483.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="LNE23_000608"
     CDS             25741..26412
                     /locus_tag="LNE23_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBK6930484.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="LNE23_000609"
     CDS             26378..26866
                     /locus_tag="LNE23_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBK6930485.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="LNE23_000610"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="LNE23_000610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBK6930486.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="LNE23_000611"
     CDS             27827..28591
                     /locus_tag="LNE23_000611"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBK6930487.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="LNE23_000612"
     CDS             28824..31037
                     /locus_tag="LNE23_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBK6930488.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="LNE23_000613"
     CDS             31048..31494
                     /locus_tag="LNE23_000613"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBK6930489.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="LNE23_000614"
     CDS             31665..32924
                     /locus_tag="LNE23_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBK6930490.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="LNE23_000615"
     CDS             complement(33013..33960)
                     /locus_tag="LNE23_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6930491.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFHIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="LNE23_000616"
     CDS             34354..35646
                     /locus_tag="LNE23_000616"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBK6930492.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="LNE23_000617"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="LNE23_000617"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBK6930493.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="LNE23_000618"
     CDS             37539..38414
                     /locus_tag="LNE23_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6930494.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="LNE23_000619"
     CDS             38680..40563
                     /locus_tag="LNE23_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK6930495.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="LNE23_000620"
     CDS             complement(40638..40922)
                     /locus_tag="LNE23_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK6930496.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="LNE23_000621"
     CDS             complement(40912..41646)
                     /locus_tag="LNE23_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBK6930497.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="LNE23_000622"
     CDS             41775..42398
                     /locus_tag="LNE23_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBK6930498.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="LNE23_000623"
     CDS             42590..43711
                     /locus_tag="LNE23_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBK6930499.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="LNE23_000624"
     CDS             43718..46846
                     /locus_tag="LNE23_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBK6930500.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="LNE23_000625"
     CDS             46865..50059
                     /locus_tag="LNE23_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBK6930501.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="LNE23_000626"
     CDS             complement(50205..50912)
                     /locus_tag="LNE23_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK6930502.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16782 a         8954 c        10639 g        14905 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat gaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta atcattgctt taaaatacta
//
LOCUS       DAFCNS010000011        48645 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFCNS010000011 DAFCNS010000000
VERSION     DAFCNS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.011"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            159..3437
                     /locus_tag="LNE23_000627"
     CDS             159..3437
                     /locus_tag="LNE23_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBK6930503.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3978..5885)
                     /locus_tag="LNE23_000628"
     CDS             complement(3978..5885)
                     /locus_tag="LNE23_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBK6930504.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6015..6890
                     /locus_tag="LNE23_000629"
     CDS             6015..6890
                     /locus_tag="LNE23_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBK6930505.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6970..7944
                     /locus_tag="LNE23_000630"
     CDS             6970..7944
                     /locus_tag="LNE23_000630"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK6930506.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8124..9269
                     /locus_tag="LNE23_000631"
     CDS             8124..9269
                     /locus_tag="LNE23_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK6930507.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9406..9750
                     /locus_tag="LNE23_000632"
     CDS             9406..9750
                     /locus_tag="LNE23_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBK6930508.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10021..11307
                     /gene="efmA"
                     /locus_tag="LNE23_000633"
     CDS             10021..11307
                     /gene="efmA"
                     /locus_tag="LNE23_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBK6930509.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11445..12044)
                     /locus_tag="LNE23_000634"
     CDS             complement(11445..12044)
                     /locus_tag="LNE23_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBK6930510.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12448..13569
                     /gene="mnmA"
                     /locus_tag="LNE23_000635"
     CDS             12448..13569
                     /gene="mnmA"
                     /locus_tag="LNE23_000635"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBK6930511.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13815..15236
                     /gene="arcD"
                     /locus_tag="LNE23_000636"
     CDS             13815..15236
                     /gene="arcD"
                     /locus_tag="LNE23_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBK6930512.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15250..16368
                     /locus_tag="LNE23_000637"
     CDS             15250..16368
                     /locus_tag="LNE23_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBK6930513.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16428..17720)
                     /locus_tag="LNE23_000638"
     CDS             complement(16428..17720)
                     /locus_tag="LNE23_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK6930514.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17852..18430
                     /locus_tag="LNE23_000639"
     CDS             17852..18430
                     /locus_tag="LNE23_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6930515.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18559..18936
                     /locus_tag="LNE23_000640"
     CDS             18559..18936
                     /locus_tag="LNE23_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBK6930516.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18869..20941
                     /locus_tag="LNE23_000641"
     CDS             18869..20941
                     /locus_tag="LNE23_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK6930517.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21152..22099)
                     /locus_tag="LNE23_000642"
     CDS             complement(21152..22099)
                     /locus_tag="LNE23_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6930518.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22345..22809
                     /locus_tag="LNE23_000643"
     CDS             22345..22809
                     /locus_tag="LNE23_000643"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBK6930519.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22871..23914)
                     /locus_tag="LNE23_000644"
     CDS             complement(22871..23914)
                     /locus_tag="LNE23_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBK6930520.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24291..25256)
                     /locus_tag="LNE23_000645"
     CDS             complement(24291..25256)
                     /locus_tag="LNE23_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBK6930521.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFTLSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25490..26335
                     /locus_tag="LNE23_000646"
     CDS             25490..26335
                     /locus_tag="LNE23_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6930522.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26526..27713
                     /locus_tag="LNE23_000647"
     CDS             26526..27713
                     /locus_tag="LNE23_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK6930523.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27719..28075
                     /locus_tag="LNE23_000648"
     CDS             27719..28075
                     /locus_tag="LNE23_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBK6930524.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28077..28415
                     /locus_tag="LNE23_000649"
     CDS             28077..28415
                     /locus_tag="LNE23_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBK6930525.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28491..28747
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28810..29844
                     /locus_tag="LNE23_000650"
     CDS             28810..29844
                     /locus_tag="LNE23_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6930526.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29837..30523
                     /locus_tag="LNE23_000651"
     CDS             29837..30523
                     /locus_tag="LNE23_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6930527.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30547..31395
                     /locus_tag="LNE23_000652"
     CDS             30547..31395
                     /locus_tag="LNE23_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK6930528.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31592..32365
                     /gene="sufC"
                     /locus_tag="LNE23_000653"
     CDS             31592..32365
                     /gene="sufC"
                     /locus_tag="LNE23_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBK6930529.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32378..33664
                     /gene="sufD"
                     /locus_tag="LNE23_000654"
     CDS             32378..33664
                     /gene="sufD"
                     /locus_tag="LNE23_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBK6930530.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33661..34896
                     /locus_tag="LNE23_000655"
     CDS             33661..34896
                     /locus_tag="LNE23_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK6930531.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34883..35353
                     /locus_tag="LNE23_000656"
     CDS             34883..35353
                     /locus_tag="LNE23_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBK6930532.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35358..36749
                     /gene="sufB"
                     /locus_tag="LNE23_000657"
     CDS             35358..36749
                     /gene="sufB"
                     /locus_tag="LNE23_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBK6930533.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36845..37987)
                     /locus_tag="LNE23_000658"
     CDS             complement(36845..37987)
                     /locus_tag="LNE23_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6930534.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWIAHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNDLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGNVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(38026..38760)
                     /locus_tag="LNE23_000659"
     CDS             complement(38026..38760)
                     /locus_tag="LNE23_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6930535.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLRNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            38992..39267
                     /locus_tag="LNE23_000660"
     CDS             38992..39267
                     /locus_tag="LNE23_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930536.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMEQYKG"
     gene            39365..41362
                     /locus_tag="LNE23_000661"
     CDS             39365..41362
                     /locus_tag="LNE23_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930537.1"
                     /translation="METPMINIPINVDKERLLEKPVIEDEKKRIRKRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNKSEEFLETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKALSINLVHNSAFAIQKSIRYSENLEKFKIVFLLKEAITDYSKMVAVYEYLKKI
                     IPGCDDNVNASNRMFFGGYKSDAVIMINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMDKLLKSSKNL
                     RCLFHDDHNPSASIFTSKTGHSIFYCHSSNCSISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMEDKLFRTIRNYLEEMRHVYDILLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGVVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(41438..41773)
                     /locus_tag="LNE23_000662"
     CDS             complement(41438..41773)
                     /locus_tag="LNE23_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6930538.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            41901..42116
                     /locus_tag="LNE23_000663"
     CDS             41901..42116
                     /locus_tag="LNE23_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6930539.1"
                     /translation="MKKVSNLSRYRKEKGFSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            42153..42515
                     /locus_tag="LNE23_000664"
     CDS             42153..42515
                     /locus_tag="LNE23_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBK6930540.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            42561..42887
                     /locus_tag="LNE23_000665"
     CDS             42561..42887
                     /locus_tag="LNE23_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930541.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYSLNRTCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYT
                     EVLMLN"
     gene            43374..43511
                     /locus_tag="LNE23_000666"
     CDS             43374..43511
                     /locus_tag="LNE23_000666"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930542.1"
                     /translation="MAHNERFKQEIIQELEKGVQHQKLISKTAQKFDISLAKEVKLHE
                     V"
     gene            43576..44358
                     /locus_tag="LNE23_000667"
     CDS             43576..44358
                     /locus_tag="LNE23_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930543.1"
                     /translation="MKNSSNSAEVNVSTSTSEAVQCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDGVKYSEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKKVSGENNETEAVTEQTMDERIDSSESNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKNVPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(44852..46141)
                     /gene="dcm"
                     /locus_tag="LNE23_000668"
     CDS             complement(44852..46141)
                     /gene="dcm"
                     /locus_tag="LNE23_000668"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBK6930544.1"
                     /translation="MFANMSETLNVLELFAGVGGFRVGLEHSNADLFKTKWANQWEPS
                     RKSQDAFEVYDYRFPNSENINRNIEEISNEEFEQMDADIIVGGFPCQDYSVARSKKHE
                     MGIEGKKGVLFWQIIRATNHIKPKYLILENVDRLLKSPSKQRGRDFAIMLASFNDLGY
                     SVEWRVINAADYGRRQRRRRVFFFVYKNDTGFANKIDARFEQKILGEEEHRYDEYIFS
                     EGLFARQFPIIKTPYKGRQASYKLSNDIVEISDNFTGKVYNTGVMRHGRYFTIDTEPY
                     DKEKPTPLREIIQPEDEVAEKYYLTDESKIKKFEYLRGPKKIERVTAEGHTYTFSEGG
                     MSPYDDLDMPGRTMLTSEGSVNRSTHLLKIDGRYRLLTPIEAERLQDFPDDWTKYKKL
                     SSGEIVEVSDRMRLFFMGNALVTKIVERIGDELQQLL"
     gene            46352..47830
                     /locus_tag="LNE23_000669"
     CDS             46352..47830
                     /locus_tag="LNE23_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutH"
                     /protein_id="HBK6930545.1"
                     /translation="MSEYLTKEQVHNRAKEAVGKSMIELNNGIPLLNSKNKNSIGDIF
                     ESWFGKVKDSESRPDLEEAGVELKATPIKKLKNGQYSAKERLVLNIINYNEVAEETFE
                     ESHFLYKNNIMEIAFYEYLKDTPKDQWTIREAILYEMYKNPVDFEIIRQDWELIKKYI
                     LDGKAHELSESLTNYLAPCTKGTNAVKSLRTQPYSEQKAKQRAYSLKSGYMTSILRKY
                     VLGDEKIDSIVKNTFQLEENNLEDLVLEHFIPYVGQTVQSLCEKFTIKTSYQANYRIV
                     SSILGLSGTVTKGEPFPRVEEFEKASILVKTIYFDKSGNNKESMSFPSFNFIDLSKQV
                     WEKEDGEPSSDWHNFLLDTRFLFVVFKEEDEGVVFKGAKFFSMPEEDIEGPVRKVWED
                     TVSKLREGVELEAIPDKSTKDGWKIKNNFIKKTDKMICHVRPHASQRDYSVDGRYADK
                     LPVPAKWINKPDNDNYSDQWMTKQCFWINNDYIKEQVQDLIS"
     gene            complement(47844..48275)
                     /locus_tag="LNE23_000670"
     CDS             complement(47844..48275)
                     /locus_tag="LNE23_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HBK6930546.1"
                     /translation="MKHRQLSTTPEVSKRMANVRLKGGKAESLLAKELWHQGVRYRKN
                     YKKLPGSPDIAVTKYKLAIFVDGEFWHGHDWDNRKQKLKRNRDYWIEKIEENMRRDER
                     NNQSLIKLGWVPIHFWEKDILKNLNYCTSVILDYIAQKKEE"
BASE COUNT        16012 a         8591 c        10036 g        14006 t
ORIGIN      
        1 tttttccgtt aataaatctc tttattatca acaacttaag ttatattttt tctttttata
       61 aaatacactt attataattt tgttttttat tgacagtaaa aataaattag gtaatacttt
      121 aagtaaacgc tttctaattt gttacgggag gaatacactt gttaaaaaaa cgtataaaat
      181 acagtgcgct aatgatgaat ctccttatgc tcagtacccc ggttttaatt tctgtcgttg
      241 aagttgctgc taacgaacaa cagtctgtga acgaagaaaa tgcacacgtg aacattgaag
      301 ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga tatggatacg gaagaaggaa
      361 atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa aaagcaatct gaagaagcaa
      421 atgaagagtg gaaaggggtt gttttcggag aaagcacaag tgctagtagg tatgccattg
      481 aaccattaga agacggcgtg cggctctctt ccacgaataa cggcggaaaa ttccagtcaa
      541 gcggatcaga tggaatgacc tattattatc aagcaatccc caaagacatc aactttagga
      601 tgcgggcgac gatcgaaata gaatcttgga cctatacgaa tgcccaagaa ggatttgctt
      661 taatggtaag agacgcagta ccaaaaaatc atttgtttgg gcaggcattt tattcaaatt
      721 catttgccat actaggcagt cggatcgaat atgtctggga ctcggataga caagaagtgg
      781 tcaacacgag cggcagcaaa tatacgatgc gacttggatt ggggacacgg gcaatcacag
      841 gaatatcatc tgaagatccg caagctgcgc cagctcctgg gagtgtctca gtggaaacaa
      901 cacctttaga gacttcagca gggttcttgg aaaaagaaac aggttcttat aatatattcg
      961 gaaacggtca tgggaatctg tttccggcca cgaactcgat tactaaactg gacgtagaga
     1021 tgaaaaaaac gaatactggt ttagaggcat attattacga tcctgaaaca ggagaagaaa
     1081 tggcttctga catcatgtat gactgggaaa aattatacgc ttcagatgaa tcggttattt
     1141 atgcaggatt tgctgcagca aggaatatga cgatcaaggt agaaggaatc gaagttgctt
     1201 actcagatcc tgcgacagat cctcctgggc aagaaagacc tactcgcttg attgatccga
     1261 tttacactgt gacctctagt aatcattcag gaaaccaaga tcatactatg tcctttagag
     1321 caaatgctga cggattattg acaattaaaa ataaagcaac aggagaggtg cttaagaacg
     1381 caaaaaactt ggctatcact gcgaatcggg aatttgatta tcagacaaaa ctgatggaag
     1441 gaacgaacga atttactttc agttttacgc cagataaaaa ctatccgcca gaagaatacg
     1501 tagagatgac tagttatgaa acaaaagaga ttcttcatac agtggactac cgtaaaccta
     1561 aatattcgac agtttatgta acaccagaag ggtcagatgc aggaaacgga agcagacaaa
     1621 atccattatc tctgacacaa gcattgagat acgcatatgc tgggcagaca atcgtcatgg
     1681 cgcctggaac ttattccttc gaaagaggat tgacgattcc aaaaggcgtt aacgggacaa
     1741 gagaaaaccc gattcagttg attgcagaga aaaatccaga aaacaagcgt cctgtacttg
     1801 attttaaagg aactggaaat gggatgacac tgttcagtca ctactggggt ttacgagggt
     1861 ttgacattac aaatagtgca gctatgcaaa aaggattaca gatttctgga aatcataatt
     1921 tgatcgaaga gattcatgct tatcggaatg gaaatacagg aatccaaatc agcggtagct
     1981 caaatgatcc tttcgaagca tggcccgcgc ataacttagt caagaactgt acatcttttg
     2041 aaaatgccga tccaggattt gaagatgccg atggatttgc ggccaaactg acaataggag
     2101 aaggaaatgt atttgacggt tgtatcgcgt atcataatgc agatgacggt tgggaccttt
     2161 ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat caaaaactca gttgcttatc
     2221 gaaacggctg ggtaccagga attgagggag aaggtaacgg taatggattc aaaatgggcg
     2281 gttcttcttt aacgggccct catgaattga tcaatagttt gtctttcgaa aatcttgcga
     2341 aaggaatcga ttccaatagc ggaacagata ttatcgtcaa tagcagtaca agttttaata
     2401 acggttcata taatgtggct ctttatacct cttcagcagg aaacacagac taccatgctt
     2461 ctggtatctt atctttccga acagagaatc ttgagatgag agagcagatc cgcccattga
     2521 atcaaaacga agatcaaatc taccactcgt tgaactacta ttggaatgaa cgagagaaac
     2581 aatcagaaaa tactcttgga cataccatat caaaagattg gatcgagtct ctaaatacat
     2641 gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa tggaagtatc catgttcatg
     2701 gactgatgca gattaaaccg gggaatcgtc aaacagaaaa ggaaagagtg gatggtcttc
     2761 cattatctgt aggtgctatt tttgacggag aaacctcacc gtcttcggaa tatccagagt
     2821 atgcaatcgt acaagaacca aatcagacaa aaccaaatga acaaaataat ccgattatta
     2881 tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa tatcattgaa ctgaagcctt
     2941 ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac gcctgtcttt gaagatatgc
     3001 gttatttccc agaaaagatc caaatcgttg aaacacagtc aagtgctcaa ttttctaatc
     3061 ataccgctat tttttcagca ccttcaaaag gagaatatac gctagaagtc acttatcgtt
     3121 cggaaatatt tgatggggaa aaatggtctg ctcatacaga atctgttacc cagcaaaaag
     3181 cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa tgacaaacca gaaagagaaa
     3241 atccaggaaa tgaatcacca aatggaaaac caaatggaaa accttctcat ccggatagga
     3301 cagcaaaaga aagtgaaaaa aatggcactc aaggactccc aaaaacggga gaatttaata
     3361 acccgttgct agcattagct ggaggaattt tgttaatcgg tgtagttgtt tatgtcatga
     3421 aacagcgaaa aaaataagtg gaatttctcc ttttatgatg taaaacaatt gactgaggaa
     3481 agtaaaaata gaaggaaatg agaccaatag atgtctcact tccttctatc tttttataga
     3541 tgaggttctc cttgctgctt ttgattggct gttcttatta cttgatgcag gacagctttt
     3601 tcacaatctc tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaacaaac
     3661 ggcaaaatat gaggaactat ttttccgttt ttttctctaa tactcaggtc aggacgctga
     3721 tttcacaacc tctcgataaa cggtgttcaa aagccagttt ctcggtagta agtcaaattt
     3781 atgcaaaaat gtggaaagcc attttcacaa aatttttctt actacttgaa actaagcagc
     3841 tttttcacaa cctctaatac atggtattcc agaagtaact tcttcgaaaa ataagccgaa
     3901 atgtcacaaa aatttgagaa gcgattttcc caaattcctt cttatttctt gaagctgacc
     3961 acttctggca caacctctta ccagattcgc actctttttt ctggcgctag atacatagcg
     4021 tcttgttccg tgataccaaa tgtttcatag aattcgtcaa ggttttttgg ttgcacgttt
     4081 gctcggagtt tagcaggggc atggacatcg atttgcagta aaagttgttg gtattctttt
     4141 tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga attcctcgat agaaagatct
     4201 gattccgttt ttgctgcttc caatgcacag cttagaccgc ctgcatcagc aatattttcc
     4261 gaaacggtta attttccgtt tacttttccg tcagcaaaag gcagaccatc gaattcttcg
     4321 atcattgctt gtgctttgtt ttgaaattgt gtaagatctt cttctgtcca ccagttgttc
     4381 aagttgccat attcgtcaaa taaagcgccg ttattatcaa atgcatggga aatctcatga
     4441 gcgataacag ctccgattcc accataatta gcagagcttg attgttctag actgtaaaat
     4501 ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc gaaatggatg ataataagca
     4561 ttgaccgtgt cagcactcat ttcccattct gaacgatcca ctggtttgtt ccatttgctg
     4621 aaacgatctt ttaatgtgag tttgctgaaa acaagagtat ttgaaagcaa agtaccaccc
     4681 tgatctgcag gtactgtttt gaactggtcg aataatgctg ggatcttatc ggggtagcct
     4741 acttggatac ctaagttatt taattttatg acggcttttt cttttgttgc atccccaagc
     4801 cagtcatttt cttctagacg ttttttataa acggcgatca tttttttcac catttgttct
     4861 acgtcttttt tagcagcttc gccaaaatat ttttttccgt agtaatcgcc aactacttga
     4921 tcgaattgtc cagaggcaag gtaatacgct gcttttttct gaggcattgc ttcgtctgtt
     4981 ccagacaata cgcgagaata gattccgctg acttggcgga attcttctga tagataacca
     5041 ctaagagaac gtactgtttt aatgatcatc cagcttttga ataacggaaa atgagcttct
     5101 gtcaggatgt cattcaaatg atcgaagtac actggatcag ttacgatcac tttatctgga
     5161 gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca gtttatctgt gtatttaata
     5221 aatgtatcaa aagattgcgg attatacatt ttgctgtaat ctgcgttttc ttctgcactt
     5281 tttacatgag gagcaatcaa tttatcaaat tgcactgctt gttccgtgat tgcttctgcc
     5341 tgactttttt ctttgccggc aagctctaat aactgaacag ccatatcaaa gaaaacgcct
     5401 attagttgtg caccgttggg atggtctgat gcataatacg tcttatctgg aaggaataaa
     5461 gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct tcatatctgc atctacatcc
     5521 agattaaatg gcaatggtaa tgtatccaac agccattctg gaaattgctt attcaattcg
     5581 tcgaaagact gcaatgattc aactttttcg attataggtt gaagcggttg agcatgcaac
     5641 tcatttcttc ggtcataatc gtttgccagt tgataatatg caaggaaatg tttcattttt
     5701 tctgaagaca ctttttctgg atgggcaagc agatcgtctg tttctcccat caaaagttgg
     5761 tcgatcccgt caactaaatc ttggaaaccg cctgtagctg gtttatctgc agggattttt
     5821 gctgtttttt gccattcttc attgactgct tcatagaaat cttgtttcaa tcgttctttg
     5881 ttcattcatc gatcgctcct tttttgtttc gtgtgcccat tatcttacaa aatgaaggaa
     5941 ttaacaaata gaacataaaa aattgaaaat tcgaacaatc aaactgattt gtttttttca
     6001 atccttcact tataatgaaa aaaaggaggc gatcccaact gaatcaagta gtggataaac
     6061 ctttatattt caaagtagat aaaaaaatta aaaaattagc cagcactcag cagctgcagt
     6121 caagaaaaag taaattgctt tttttagctc tggtttttga agatcaaagt tatgtgatta
     6181 tcgatcaatc aggacatccg gtagaatatt ctccagcaaa atatacttat caagagggtc
     6241 tttcttgtaa aaaatggaag ctgatcaatg aagagccaat cgagttatct agatggatca
     6301 acagaaaaga agatattccc gttttgatcg aagaaaaaag gtcaggaaag gaactcgcaa
     6361 attgctgggt gggattgcca gaagaacgtt ttttgaggta taagaaatgg gctaccccct
     6421 caggctatct ttgtggtact tacgcggcag cagttctcct tgcctattat caagactatc
     6481 gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa tacggctgat tcgttggttt
     6541 tgacgaaggc attgcgtagc cagatccaac cactaggact tcctacgatt ccttttcagg
     6601 ttagtacagg gatttctagt tttttaaaga agaatggcaa tcatgagcgt gcacgagcaa
     6661 ctcttctagg ttcatggcag cgagctacaa aacgtatacg tgaaggcaaa cctgtgatga
     6721 ttggtatttt gaaagtactt ggcagtacat atggcaatca ttgggtgaca gcgtacgctt
     6781 attttgaaac tgaaacagga gaacgttatt acaaagtaca cgacaactgg ggcgattatc
     6841 ataaagtcat tcctgccagt tggagtaacg gaacagtttc acttccttaa gaatttgctg
     6901 aaattaacag aaaatcttgg aaaaatatgt taaactatcg aagaatagag aagttttggg
     6961 gaggactaca tgagtaataa gtatcaggat gacacgttga agatttttag tttgaatggc
     7021 aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa cagaattagg taaaagcgtc
     7081 gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg aagaaagtat tcgaggggat
     7141 catgtgtata tcgttcaatc aacgaatgca cctgtcaatg attactatat ggaactactg
     7201 atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga tcaatgtcgt attgccttac
     7261 tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg aaccgatcac tgcgaaattg
     7321 atcgctaact tgatcgaaga agcaggggca actcgagtac tgactttaga tctgcacacg
     7381 gttcaagtac aaggattctt tgatattcct gtagataatt tatttacgat gccgttgttt
     7441 gctcattatt accgtcaatt aggtctgacg ggagatgaca tcgtagttgt ttcgccaaaa
     7501 aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt tgaacagcac attagccatc
     7561 gtagatcatg cagatgatgc ttcaactgag ggtggttatg tgatcggtga tgttcaagga
     7621 aagacatgta tcatggtaga tgatatcttg aacacaggag caacgcttgc acgagcagca
     7681 aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct gtgcgtcaca tggattattg
     7741 tccgatcatg cgaaaagtat tttagacaat gcaccaatca aagatatttg tatcactgat
     7801 tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa caatcgttac atgttcagaa
     7861 ttaatgggtg aagcggtcaa acgcatccat gaaaatacac cgatgagtcc gttattccgt
     7921 ctggaagaaa aagaattcga ataaacaaaa aaaagaggga aagaagttgt gacagaagtg
     7981 agcagcttca aggagctgcg acaagacttt tgtcacagct ccttttttct atgcccatga
     8041 aacgtcctct agacaaaaat gagctcttct ttggtttgtt cgctatttta tgctagaatg
     8101 tgcctagcaa taatttggaa gggatgaatg aagtgaaaac tatttatctg gatcatgcgg
     8161 caactacccc catgcatcca caagtaattg aagcgatgac ggcaatcatg caagacacgt
     8221 ttggaaatcc ttcaagcata catggatttg gtcgccacgc gcatgaaaaa ttggaaacag
     8281 caagacaaat cattgcagac agtctgcaag caaaacctca cgagatcatc tttaacagtg
     8341 ggggaacaga aggggataat acagctatct tagaaacagc gttctcgcgt caaaaagaag
     8401 gcagacatct aatcactaca gctatcgaac atcctgctgt tcttaatacc atgaaatatt
     8461 tagagacaaa ggggtttgac gttacatatc tgcctgtaga tgaaaaaggg aatctgtcga
     8521 tcgaagaggt caaaaaagcg ttacgagaag atacgatctt ggtctctgtg atgtacggga
     8581 acaatgaaat cggaaatttg atgccaatca aagaaatcgg tgaactttta gcggatcatc
     8641 cagcttattt ccatacagat gcggtacaag cgtacggaaa ccaaacgatc catccagaag
     8701 aattaggaat cgatttatta agtatctctg ctcataaaat caatgggcca aaaggcgtcg
     8761 gtttcttata taaacgagac ggagtaaata tccctgcatt gcttttaggc ggggagcagg
     8821 aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat ctggggtatg gcaaaagcag
     8881 ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa caaatataaa ggattccagc
     8941 aattgattct tgatacatta gaagcaaacg aaatccctta tcaagtaaat ggtgatttaa
     9001 cgaataaact ccctcatgtg ttgaatcttc gcttaattgg cgaagcaaat gatttgctac
     9061 tgatgaagct tgatcttaat ggggtcgcta tttctactgg ttcagcttgt acagcaggaa
     9121 atatcgagcc ttcacacgta ttagaagcaa tgtatggaga agatacaccg atactaaaag
     9181 aatccatccg tatcagcttt ggtttgggaa ataccgaaga cgacatccag tatttccttg
     9241 atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc attcttatat gaacagatgt
     9301 ctgagagaac gccttcattt tttagggaac ttaactgaaa aacctagtaa aaatcaggat
     9361 aaacttttat aataaagaga aactaatttt atcgaggtga aaatcatggc atttgaaaca
     9421 acagcaacgg tactgggcgc accaattagc tatcgtttgc atcctgatgc aaaaagatat
     9481 acattgcgcg ataatggatt tacagaaaca aacggaggca attatcaatt gatccgacca
     9541 ttagatgcca cgccgcaaag caaagaagga ttcaaattaa aaatcactgt atcaaaagat
     9601 attcaaacat tgaaaatgtc gatcactaca gcaaatggat tgaaagcagt caatattttc
     9661 aaagatccaa aacagaaaat gagccaagat aaattttatt ttctgatgga tgggatgatt
     9721 agccgtgggt tattcgaaaa agtcgaatga tctgtggata taaaaagaca actagttttg
     9781 aaacggccta ttgttagatg actaactttg taaggacgac gcaaattagt tgtctttttt
     9841 atttttaaat ttaacacatg ttgttatttt taatattttg ctgatttatt gtttctaagt
     9901 aaagaaattt aaattgaaac gattgttatt tagggaaaaa ggtatttttt gaaaaaagaa
     9961 tcccgtataa tgattgttgt gcaaacgcta tacaaaatga aaaagaaaag agggatgttt
    10021 atggaaaatg aacagtcggt cgttttaacg aattggaaga gaaattattt gttcttttta
    10081 tcgggacagt ttttgtcagg aatcactagt atggtcgtcc aatatgcgat catctggtat
    10141 ttgacaagag aaaccggttc ggcgacaatt ttgagttttg ctactttact aggaatgatt
    10201 cccatggttt tactaagccc atttgtagga ccgctagtgg atcgctggga taaaaaagct
    10261 cttttgattg tcacggatat tattgtagcg attttcgcgc ttattttagc agtagtcgga
    10321 acgatttcag aatccttccc gatttggcta gtttttgtgt ctttgtttat gcgttcggtc
    10381 gcacaaacat tccaaatgcc aacgatccag tcgattatgc cgacaatcgt tccatcttct
    10441 cacataacaa ggacgaatgg acagttagga atggtccaat cagcgaattt catcattgcg
    10501 ccagctctag gtgcggcttt gttttcagtc gttcctgtca attaccttat tttattagat
    10561 gtattggggg cagtttttgg tgtcggctta ttgatttttg tgaagatccc aaaagtttct
    10621 cctgaaatat tggaagttcc gcttactatt tttaaagatg caaaatttgg gctgcagcaa
    10681 ttgatggata ataaagggtt atggtacata acgatcaatg gtgcatttgt gatgctgttg
    10741 ttcatgccag caatcagtct gtatccattg atgacgctag attactttgg tggttccgtt
    10801 ggacaggcgg gagcagtgga agtagtctac gcagtcggaa tgctcttagg cggtgcactg
    10861 atcagtttta taggaacatg gaaagaccga atgaaaccta ttattatagc ctacatcatt
    10921 atgggcctga cgatcggtgc aagcggattg gttccaaacg atagtcaagg gttcttgtac
    10981 tttcttatct tgaatgcagg agcaggttgt gcaacgccat atttcaatac actgctgatg
    11041 gcaatgatcc agcagagtta tgaatcgaat gttttaggtc gtgtcttagg gaacttcaac
    11101 tctttgatga acctggcagg tccaattgga ttattatttg caggaccatt agctgatcgt
    11161 ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga ttttattatg cggaatcgtt
    11221 ttatttttaa cgtcagctgc ccgaaattat gataaagaat tacagaaaaa actggtaaaa
    11281 gaacaccatg agcaaaaaga cgaatagtat ggaatggagt tgtgacagaa gtcttgttac
    11341 aactccattt ctgatcctaa tgttcctgtg gcaataagcc tactcaaatg aaaaatatga
    11401 gtaattatct ttatttgttt aatacttgat atagggcagt tttttcacga cctatcgtac
    11461 ttcctctttg cttcatagat ttctttgatt tgttgctcag ataacggagt gccaaatggt
    11521 agtttttcct ttaagagttc tctttccggt gcgtctacgc ctacattgac atcgttctcg
    11581 ataggtacgc tgtaatgatg gatatgtcca tgaagattgc ggatagtgtc gttaggcccg
    11641 agcaacatcg gataatgagt caagtaatat tgttgatggt cgaatttaac gatggcacct
    11701 acatcataaa aataaaattt gggacttccg tcagtaagcc taggatcatt tttttctaaa
    11761 taacggaaaa aagcgcgact gtcatggttc cctttgatca agtggattgt tccttgcagt
    11821 tgagtgacca gttccaaaat ttctgcattt gctttttcat attgcggatg catagcaagg
    11881 tcgcctagat gataaacatg gtcggtttct tttattactg cattccagct agtgatcaac
    11941 tgttggttca tttcatccac atttgcaaag agccgcggag caaagtcgtt ttttcctaat
    12001 aagcgttcat ggaaaaagtg catatcactg ataaagtaat tcatgtatct tttgcctccc
    12061 ttttttctta gagtaaacaa aatcacttat ctgagcaact cataacatct aaaaagaaaa
    12121 ggttgtaaat gagacgtgca gctttaagcg ataagaagca attttgaaaa atagtttttc
    12181 acaatttata aaattattcc ttattgccga aaggctggcc ctttttatga gtatgccaat
    12241 tttttatatc gaatgctcaa aagatgagtg attttttggt tgactatcat gtttgtgtac
    12301 tgaataggtc cagtagaaca gtatttttac gacttgataa ttaagggatg aaattttcag
    12361 aaatatgacg tataatgtaa aatactgaaa agttgcgacc ttcaactcgt agatgtttca
    12421 gaatctaatt gaaatgatgg tgatatcatg acagacaaca gcaaaacacg cgttgtcgta
    12481 gggatgagcg gcggtgtcga ctcatctgta acagcacttt tgttaaagga acaaggatac
    12541 gacgtgatcg gaatattcat gaaaaattgg gatgacacgg acgaaaacgg tgtatgcacg
    12601 gccacggaag attataagga tgtagcgaaa gtagctgctc aaatcgggat tccttattac
    12661 tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg aatatttcct agccgaatac
    12721 cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca aagaaatcaa attcaaagca
    12781 ttcttggatt atgcaatgga tcttggcgcc gattatgtag ctactggaca ttatgcgcaa
    12841 gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg gagtagacaa taataaagat
    12901 caaacttact tccttagcca attatcacaa gaacagcttt caaaaacgat gttccctcta
    12961 ggcggcatgg aaaaatcaga agtacgggca attgctgaac gcgcaggcct tgcgactgct
    13021 aagaaaaaag attcaacggg aatctgcttt attggtgaaa agaacttcaa acaatttttg
    13081 agcaactatc tgccagcaaa aaaaggaaat atggtcactc ttgatggtga agtcaaagga
    13141 caacatgctg gcttgatgta ttatacgatt ggtcaacgtc aaggactagg gatcggtggc
    13201 ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag atctagcgac aaacacatta
    13261 tatgtggggc aagggttcca tcatcctgca ttgtatgcca caagcttgga tgcaagtgaa
    13321 atccacttta cgacgaatga accaatgcca aaagaattca agtgtacagc gaaattccgt
    13381 tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg atgataatcg tgcagaagtc
    13441 gtattcgacg aacctgtacg tgcaatcact cctggacaag cagttgtatt ttatgatgga
    13501 atggaatgtc tgggtggtgg tttgatcgac cacgcatatc aagaaacaaa agttttacaa
    13561 tacgtttaag cgatattaaa tgaatcaaaa cagctatggc taacaaaaga aacacctttt
    13621 cttttggata gcatagctgt ttttttgcaa taaaattaat ttttattcat tttatacttg
    13681 aaatacttgt gtaaaaagtt cataatatcc aataatatga ataaagttgc tttttgttaa
    13741 gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt gatttgttca attttataag
    13801 tgagggtgat ggaaatggaa aagaaatcga cgggtattag caaatgggga ctggttgctc
    13861 tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat cacaagtgat ctggctagtt
    13921 ctgcagctcc agggccagcg atcctttcat ggataatcgt tgggattggt attttagctt
    13981 tggtattatc gctgaatcat ttaggagaaa agcgtccaga tctagatggt ggtattttcg
    14041 gttacgcgga agcttcattt ggtaaattag gtggcttcat cagtggttgg ggttactggt
    14101 tgtctgcgtg gctgggaaat gttgcttttg caacgatgct gatgagcgcc atgggagaat
    14161 ttttcccgat attcaaaggt ggacaaaatg ttccttctat tttatttgca agtattttta
    14221 tttggggtct gactttatta gtaaacaatg gagttgaagg agcgagcgtc atcaatacaa
    14281 tcgtgacgat ctgtaaatta gtgccattat tcctattttt aatatttgta tttatcgcat
    14341 ttaaagtgaa tatattcact gctgattttt ggggtaacgt atctgataat ctagtgaatg
    14401 gaacagggca gcaaggaacg atctggttgc agattaaagg ttgtttgatg gttatgatgt
    14461 gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa ccgcgcgaag aaacgttcag
    14521 aagcccagca agcatcgatc attggtctgc tttgtttatt gtttatctat atccttgctt
    14581 cgatcttgcc atacggcgtc ttatctcaag aagagttagc aactatttca caacctgcta
    14641 tggcaaatat tttaaaagga atcgtgggaa attggggagc agcgctgatc aatatcggct
    14701 tgattatttc gatcattggt tcttggttgt cttggacgat gctcccagca gaaacaacga
    14761 tgttgatggc tcgagacggt ctgttgccga aaggttgggg aaaaacgaat aaaaaaaatg
    14821 ccccaactta ttcgttagtt gttactgcaa tcttgactaa tctattctta ttgactttct
    14881 tagttactga ttatgcctat caatttgcct attctttatg tacagcggca attttgattt
    14941 gttatctact tgtcgggatc tatcaagtcc agttttcttt aaaagaagga atcaaaaaac
    15001 aagtagtgat cggtgggatt gctgtagttt ttgaattgat gggcatcatt ttagcaggat
    15061 tttcttatgt ccttttatgt agtattgcct atcttcctgg tttttgcttc tactggatcg
    15121 cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa agtaatgatt gggctgattt
    15181 gtgtggccgc actgctttca atcgggttgc ttgctgtagg ctggatcaag atataagggg
    15241 gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat ggctaaataa atgggaaaag
    15301 aaagcggtat atgatattgc acaaagctcg atcgaagcat tgacattaga cgaactcgtt
    15361 ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac gcaaaaacga accattggac
    15421 tatggctgga tcgaaggatc agatgttttc aaacaagaag tcgcttctct ttatcaaacc
    15481 gtcgatccgg aaaacatctt acaaacgaat ggagcgactg gtgctaatct tttagctcta
    15541 tacgcgttag tggaagcagg agaccatgtt gtttcgatgc ttcctactta tcagcaatta
    15601 tatgacatcc ccaaatcttt aggtgccgct gttgactttg ttcatttgaa ggaagaagaa
    15661 gattggcagt ttgatttaga acagctagaa gccttagtca aacctgaaac aaaaatcatc
    15721 tgtttgaata gtgcaaacaa tcctacaggc acattgctag atcggaaaac tttagaaaaa
    15781 atcgcagaaa ttgctcgtac agtagatgca tatgtattga tagatgaagt atatgcgcct
    15841 ttaacggata aaggtgaatt cttgtcgatc gtagatgtct atgaaaaagg tatagcaacc
    15901 aattccctat ccaaaaccta ctccattcca ggtattcgta ttggctggac agctactgga
    15961 cctgagttgg cagaagtttt tcgaaagtat cgagactaca cgatgatttg cggcggagtc
    16021 ctgtcagatg atcttgctgt acatgcattg aagaataaag aaaaaatatt ggcgcgaaac
    16081 aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat gggtagccaa tgaaccgaaa
    16141 gttgaacttg tcgctcccaa ttacgtctct acttctttca ttaaattagc catcgaagaa
    16201 gatgaccaga cattttgtat caatttatta gaagaaacag gagtactatt agtaccagga
    16261 agtgcctttg atttaccgaa gcatgcccga ctaggttact gttgtaaaaa agaaaccttg
    16321 gaaaagggat tattcctctt atctgagttc ttgaaaaaac aggcctaaac aaaaacactg
    16381 caaggacagt ctgtgacaac tgttcaagca gtgtttttca tttacgctta ttgtctatga
    16441 gaaggagagt gccctgaagc tatggagact cctgaagctt tttcttctgc agtttcttgc
    16501 tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt tgaccaaaac aagaagagaa
    16561 gtcgaaagaa agacgccgcg ataaccaaat gcactggaaa cactggagcc gatcataggc
    16621 cctactacgt taccagttgc ttggaaagat tggttataac tgaagatccg tccagcagct
    16681 tgatgaggcg aatattttgt gatcaatgct tggacagcag ggagcagaca agcatcagag
    16741 ataccaatca aaaaacgtaa ggcagctaat tgccaaacat tcgtgacgaa tgccattggg
    16801 atataaacca gaatcgcaaa acccaaacca atcgcaagta ttctttcact cccaatcttg
    16861 tcacctaagc gaccaaatct tggagcggct atcaaagtgg caatcccagg tatcgaagca
    16921 atgattccgc tgatcaacgt aacattccca tgtccatgca gcaattggcg aatgtacaga
    16981 ctgatgattg gactaatgga attgttggat gcttggatga tcatcgttgt gacgaacata
    17041 ccgatgacga cgtgtggata tttcaagtct ttgaaaatct gtttggcaga aaccatgttt
    17101 tcttttttca ctgggacaaa tttttcatgg acgaaaaaca gactcaacaa gaagacgaaa
    17161 aataagatga tccctgtaat gaaaaaggta gggcgatagc caaacgctga agcagagatc
    17221 ccgccaagaa gcggtccaag taatgttcct gtaacagaac cagttgctaa ggttccaaga
    17281 acttgaccac ttttttcttt tggcgtccca gttgcgacaa gggcagtggc attactgata
    17341 taaccagaaa aaatcccttg aagcagacgc aatgcgacta attgatacac actagtcacg
    17401 caacccatca aagaaatgac gattgccatt cctaatgaag cacgcaacag catcaatttt
    17461 cttccttttt ggtcggctaa ttttccccac caaggagaga taagcgtagt caccaagaaa
    17521 gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg acgtactata attacctaac
    17581 gtatctatgt aaagtgacat aaaaggcatg actaaactaa atccgatacc agccataaat
    17641 gttccaaacc ataagacaaa caagttttgt tcccaagttt ctcttttcct aaataatttt
    17701 tcctttattc ttgcagccaa ttagactcct tctttctgaa aattttcatc tacagctctt
    17761 tattataaac tacaatatgt agtttatcaa tgaaagtctc ttttttctct ttctttttcg
    17821 tggtaaactg aaagagtgaa ggatttattt tatgagagga aacatggaaa tgaaaaaaag
    17881 aaatctgtct caagagaaaa tcatcgactg ctttcgagaa ctagcagaag agatggatgt
    17941 tcagcaaata acctttcagc atttagccaa acgcttggat atcaaggctc cctcgctgta
    18001 taaccatttc aaaaatattc gtgatgtcaa aacagcgcta acagcgaaac ttctgcagga
    18061 acttaatgac cagctgcgac gggctcttgt gggaaaaagt ggtcaggaag cactgcagat
    18121 ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa gcagtgtatg agttactgat
    18181 cagtgtaccg cataccaatg aagctgtttt attagatgga atccatgaaa cgaatcagat
    18241 cattttgcag atatttgagg cttttcaatt aacgaaagaa gaaaaagttc atcgaagcag
    18301 ggaacttcgt agtatgatcc acggttatct ttccttacgt tttttaggat actttacaaa
    18361 agaaccaaca atcgatccag aagacagcta tttttggatg atcaataact ttatcgctac
    18421 tttgccttaa acaatcagat tcatttgtaa caattttaag atgttcatta aatgaccctt
    18481 ttataagaga aaattcttgc aactttcttg aaaagtgact aagatgagtc tagaaaagct
    18541 gaaagaagga gcacgagtat gtatcaagta attaccatgt tcggggacaa tgagccttgg
    18601 tggttttttg aagactggga agaagatatt gaagaagaag aaacattcga gtctttcgaa
    18661 gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc atcaaaatta tgaatatatc
    18721 aatgcaaaaa gtaactttct cagcgccttt tggaatgacg gagatgagcg ctggtgtgaa
    18781 gagtgtgacg atgatttaca gcaatacaag ggacttgcgt tgttgaaaaa tcatcagccc
    18841 atcacagtag aaagcagaaa ggaattttat gaaacaacta attctagcgg aaaaaccaag
    18901 cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa aagcacaaaa attattatga
    18961 aggacccaag gtgatcgtta cttgggccct tggtcattta ttaggattga aaatgccgga
    19021 agatctgaat aaggaatggc agagctggca aatggacacg ttgccaatga tccctaaaaa
    19081 actaggaatc aaaccacttc ctaaaacagg acatcaatta aaagcaatca agcagctggc
    19141 tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat gccggaagag aaggggaact
    19201 tgttgctcgc tggatcttag agtatgtgca tttcaataaa ccagtgaagc ggttatggat
    19261 ctcttctcaa acagataaag caatcaaaac tggattcaag caactaagac ctgcgaaaga
    19321 atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa gcggactggc tggtaggatt
    19381 gaatgtgacc agagccttaa ctgtgaagta cgcggataat ttatcagcag gccgtgtgca
    19441 gacaccgaca ctagcattag tgagagacca agaaaagaaa atcgagcagt tccgtccaca
    19501 aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa gccaaaatgc ttcaaaaaaa
    19561 tcaatttgct ttaaaaacac aagaagaagc acaaaaactt gttcaattga tcagtacaag
    19621 caaaggaact gtcacagaag tccaagaaaa attaaaaacg gaatctgcgc cattgcctta
    19681 tgatttaaca gagatccaaa gagaagccaa tcaacgctat ggctattctg caaagaaaac
    19741 attaagtctt gtacaaagtt tgtatgagac ccataagatc gttacttatc cgcgtacaga
    19801 cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa gaacgattgc aagcagtcag
    19861 cgactttgct cctgaggtca agcagtattt gaaaaacgga gccatcgtcc aacagacaaa
    19921 agtattccaa gacagtaaag tgaccgatca ccatgcactt ttgccaactg aaaacagagc
    19981 acgttatgaa aaattaagta atgaagagcg aaaaatctat caaatgattg tctctcgttt
    20041 tttaggactg tttgctcaac cccataaagt cagccaaaca aaagttacgg ttgaatttgg
    20101 taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa gcaggatgga aaggcgaaac
    20161 tgaaactgag acggaaacag tcaaatggga aaaaggaatg agaatcaatc ccgatttcgc
    20221 catcaaaaaa gaactatctg caccgcctaa accattgtca gaagcaagct tgcttggtca
    20281 aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca gagattatcg agaaactaat
    20341 caaatctgaa ctgatggaac gtacccctca aggactgcaa gtatctccta aaggtaagca
    20401 attattagta ttggtaaatc cctctttagt aacaccagat ttgacagaaa gctgggaaaa
    20461 agaccttgaa gctattgctg caggaaagaa acaagcagga accttcttga atggaatcga
    20521 aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc aaacaagaat atcaagattt
    20581 ctcgattacc caaaagaaat gcccagaatg tggggccaat ctgcgcgaga aaaacacacg
    20641 tgacggcaaa atctatgtct gcaccaattc agattgcaac taccgcagaa gaaaagatcc
    20701 aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa atggaaatca tcgaaggtaa
    20761 aaacggtgct tatttccgtt gtaaatacga tggaacgaca gaaaaattgc ttgataaaaa
    20821 agagcagaag aagaaaatga ccaaacatga agaacgacgc ttgctgaaaa aatatgcaca
    20881 agcagaagaa ccagaagaaa gcccattagc agcagcacta aaagcagcaa tgaagaagta
    20941 ataagatatg aaagaggttg tgatagaagt ggtcagcttc aagaaataag aaggaattta
    21001 cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat ttccgaagaa gctacttctg
    21061 gaacaccatt tattcggaaa aaaggactgt gacaagactt ttgtcacagt cctttttcat
    21121 ttaaaaaaat gtttacttca tttgacgtat attattggtg aggcttcaat tcatttggct
    21181 tcactttttc tgtttctctt atgatataaa caggacgctg tttcgtttct aagaagattt
    21241 tcccaatata tttcccgatg attccaagac ataacaattg gattccacca atgaacagga
    21301 agatcgagac catggaaggc catcctgatg taggatcacc aaacaacaat gcccgcaaaa
    21361 taatgaacaa aattgcaata ccagaaccga cacaagacaa agcgccgata aatgaagcaa
    21421 tcgttaacgg tgcatccgag aagttcacga taccatcaat cgagtagttg aataatttcc
    21481 agaaggacca agatgtttcg ccagccacac gttcgcggtt ttcataagaa atatattctg
    21541 ttttaaatcc gacccagcta aataatcctt ttgaaaatcg gttgtattct gttaattcta
    21601 ggacggcatc caccatttga cgagtcatca agcggaaatc acgagcgcca tcgaccattt
    21661 ctgtatcgct gattttattg atcaactggt agaatttttt cgcaaagaaa ctgcgtatcg
    21721 caggctcgcc ttcacgagtc gaacgccggg tgccgacaca atcgatatca gaagtagtga
    21781 tccggttgat catctcagga agcagttcgg gcggatcttg cagatcaaca tccatcacgg
    21841 tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag agccgcttct ttcccaaaat
    21901 ttctggaaaa tgagacaaag cggacataag gataagctga ggctagcttc ttgattgctg
    21961 ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata ttcgaactca tgggcacctt
    22021 tgaatttttc aacctcatca aaaaaaagag gaacagtttt ttcttcatta aaacatggga
    22081 caacaattga tactttcatc gtttctatcc tttcaatcgt atgtttatat atggtttaca
    22141 ttcaaaattt aataaacctt aaataacacc tttaaaattt gattttacgt aaaagaacag
    22201 gtattctttt tgtaaactaa cgtatttagt ttaccatgtt tcctcggttt atcaaacttt
    22261 taagaacaat ttttattagt tggcaagtag ttttggatag agaaaaagga ggaagttcca
    22321 gtctattgac taggagcaaa caaaatgaaa atacacacac ctatcggctt tttatggtta
    22381 gatgcagatg aatccggatt gactgctgta tcttttcagc caatttcaga aagcgaatca
    22441 gcgaatcagc gaattttaac gcgcacacaa gaacagtttg aagaatattt tgcgggaaaa
    22501 agaaaagaat ttgatgttcc tttatctata aagagaggta cgccttttca gcaaaatgtc
    22561 tggaaagctt tgtcgaccat tccttatgga gaaatccgaa cgtataaaga agtagccgtt
    22621 gctgttgata gtccgaaagc tgtgcgagcg attgggcagg caaatcgaac caatccattg
    22681 ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg gaaaactgac aggatatatg
    22741 ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt tgttgttaga aggatatctg
    22801 caaaattaaa aacagttgtg attcggacat tgttccatgc gagaatcaca actgtttttt
    22861 catgttcatt ctaaactttg atcagccaaa taaatagtaa agccatcact gtttggagaa
    22921 gcccgacagc taatccataa gataagaagc gtgtaccttc tttcatgaat tttgcaaagt
    22981 ctaaacgcag accaattgcc gctaaagcag tgatctcaaa ccagctgctg ataaaatgtg
    23041 ccgattggtc aataactaca ggtaaagaaa acaagctgtt gaccaaacaa aagatcacaa
    23101 accctaatac ataccaagga agcggtaatt ttcgtttttt ctgcgtatca gttggcgttt
    23161 caaacagagc tggttgtttc ttgtgcatga atttttcaaa cagatagaca acaacaacga
    23221 gcatcataat acgtagaatc ttaaatagca tggcgaattc gacaacagaa ttgtcaagta
    23281 agcttgcccc agcgactact tgaccaacag attggagtgt accacccaaa agtgcacttt
    23341 tttcaatgag atttccacca aacaactgta atccaagaaa agggagagtc aacatcatca
    23401 cagtacccag tagattgact agtgtgatga tctgtccttt ttcttcctct ttcgcatgga
    23461 tggaaggggc gattgaagca atagcagaag aaccacaaac agcgtttcct ccagccatca
    23521 tcaaagaaga agtatcgctg aattgcagtt ttttaccaat caagtaagca ccgatgatag
    23581 tgactgacat ttgcagtaac acaaaaacaa ttccttttac accaatttga gcaatcgttt
    23641 gagctgtgac agttgcacct agtaaaaaaa cagagaattc taatagtttg ctttcagcaa
    23701 attttgtgcc gattgctaga acaggctgtt taaaaaaagt atttcctaat aaaataccca
    23761 ataaaatagc aatagtcgct ccacctaact gtggcagata aatagcaaga agcctgctga
    23821 aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat ttcgcggacc gctgaatcct
    23881 ttacagattt tattggatgt gacaatgatt tcatttgatc ggttcctttc gtcaataaat
    23941 actaaaagca tactcgaaca tagtagcact aaaccagtat tttgacaaaa gaacaagagg
    24001 tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa cggtgttcca gaagtagctt
    24061 ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct
    24121 tatttcttga agctgaccac ttcttttacg acctcttggt aaacggtgtt caaaagcttg
    24181 catctgcggt aataagccca accagtacaa aaacatgaac agctgttttt gtttggccgt
    24241 tcttattact tgatgcagaa cagctttttc acaacctatt gaatcgatta ttaagcgatt
    24301 tttttagaaa caaattttcc atgtttgcta tgttctttaa ttaagaaacg tacgtaatga
    24361 gcaagaacgt agaagacaac tacacttgca aacagacatt ccgcaacttt taagaaatcc
    24421 aatacgctac tatagatacc taaactgttg aagtaatgaa cagtagtaaa tatagctgtt
    24481 gcagagataa caagcgggaa tgtaaaggct gcatagcttg gataaaaacg aagcttgaac
    24541 aaacgtggaa gcatagacag cacaacgaaa tacaatactt gagataaagt gaacaatgct
    24601 acgacaagat aaaaattagg ttcagcaaag tttttaagat aacctgttag gcaaagagaa
    24661 ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat gagccatatt tcttacgaaa
    24721 aagacacgtt tgatgatgat cggcaataag aacgcatagc agatcaatga aatccaaaag
    24781 aaaatttttc caataagtgg gaagaagttc ccggcagtta cagtgatgac cccaaatcca
    24841 acgtaaatga taaaccagct tggataaatt gcttcaattg aaagatcagc tttgataaca
    24901 tggtgataat tgaaataaaa gacaaggatc acttggaaga taacagcgca cagccaaatg
    24961 aatttgacaa ttggtgcaat actttcatat gaaacaaaat aagtacaaat aaccatgagt
    25021 gacatcgtaa aagttggtga aacggaggcg atgattggat catgcaacgt ttgtttcgtg
    25081 tgttcgaata agataaacaa tttaacaaaa atgaatatca taagaatcat agagataccg
    25141 ccagttagat tgcctaaagc aacatggtga tagtctttaa acagatttcc aagggaagct
    25201 aacccaagga tcaacccgca gatcgggatc ggaataattt tcaaaaagtt cttcatatat
    25261 aatattctcc tttttattta atagatttta gtaccaagat gaacgaatac attgtaaaat
    25321 gaataggaat gtatgattac gagcaagcag acagctgatt gtcttcatct aagttactag
    25381 ttggatcagc aactgttttg ttgtacattt ctattataga tggttttttg tatatttaaa
    25441 tcaaatagtt tttatagtaa ttatcataaa aagttatgga agtggtgcca tgtttaagtt
    25501 attaacgact tttcgagcag tatatgaaac aagaaatttt tccagagcag cagaagtact
    25561 gtttttatct caaccagcag tctcgaatca aatcaaacag ttggagcggg aattgaatat
    25621 cgatcttttt caacgtaacg gaagaaaaga gatgcttcca actaagcaag ccgacttatt
    25681 atatgaacgg acactggttt tgttagaaga atgggaagaa actcagcaaa aattgctcaa
    25741 tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc cacacctttg ctgtctatct
    25801 tttgcctcag ttgatgaaac atctgatcaa aaggtttcct aaagtgcatt ttgagatcga
    25861 aatcacgaat tctcatgaat cattagaaca agtagcgaaa catgaatccg attttggttt
    25921 tatcgaaaag ccattagaga cttccggcgt acaaagatac tctttgatgg aagaccagct
    25981 agtcatcgct ggggacccaa aaagcaaatt gtggcttgta agagaagcaa actctggtgt
    26041 ttttcattac acgcagcgtt attttgaaga gatgaatatc caagataaga agatgatcgt
    26101 gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga atcggtcgct cgattttgtc
    26161 taagcgctct gttccagaaa atgtgccaat gactccgttg aatttccatc gatattttta
    26221 tttcatccaa cgaaccaatc tggtttctga tcagctgcta gagattgccg aagagatcct
    26281 tcgatacaat gaaagcacag aaacttctca cagcgaggag aatctcagct ggtagataat
    26341 tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa gcagtaggct tttgtactgc
    26401 atttgctcaa gcaactgcac aaacggaaag ttaatttttc ccaaatgtct atggaaagac
    26461 tttccattat ctaactggac cgctataata atcagcagta tggtttttaa gaaaagggat
    26521 gaaacatgga aaaaaaatta aaagcttcga acgataatcg tattgattat ggcgtgattt
    26581 taccagtctt tctgttatgt ttgattggtc tgctctcatt atatgtagca cttagccatg
    26641 atccgaacaa tccagcagtc ttgaaaggcg tagggatgca agttgtctgg tatttagttg
    26701 gagcggcagc aatcgttgtc atcatgcatt tgaattccaa atggatctgg aaactgactc
    26761 cttaccttta tggtttgggg ttagtggtaa tgggactctt gctcaaattc tatgatcaga
    26821 atttagctgc ttcaacaggg tctaaaaact ggtttcgatt cggtgcgttt actttccagc
    26881 cagcagagtt gatgaagatt gcgtacatct tgatgatggc actgatcgta actaaacata
    26941 atacgcaagt aaaagaacgg acgatgaaat cggacttttg gctgattggg aagctcttga
    27001 tcgtgacaat accggtttta gcattgatca tggcccaaga tgactttgga acgatgcttg
    27061 tctttcttgc gatctttggc gggattttcc tgatgtctgg aatctcttgg cgcatcatcg
    27121 cacctgtagt aatcttggca gtagtcgttg gagcaggaac gatcttcttg gttacgactg
    27181 aaggcggaag agacttgctt tataaagttg gattcaaatc ttatcagttt gcacgtattg
    27241 attcatggct ggaccctttc catgatacgt caggaatgag ttatcagccg gcccaaggac
    27301 ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt caacgtaagc aatatctatg
    27361 tgcctgttcg tgagtccgat atgattttta ccgttatcgg tgaaaacttc ggctttatcg
    27421 gtggtgcatt cgtcattttc ttgtatttta ttttaatcta tcgtatgatt cgggtgtgtt
    27481 tcgatacgaa taatgaattt tacgcctata tcgcaagcgg tttgatcatg atgctgctgt
    27541 tccatgtctt tgagaatatt ggagcaaata tcggattact tcctttaaca gggatcccat
    27601 tgcctttcat tagtcaggga ggatcctcga ttttagggaa catgatcggt atcgggttga
    27661 tattatcgat gagataccaa aatgaagcac ctgcaagatc ttcaaggagg taagaaaatt
    27721 gtacaccttt tactggtacc cgaaatgttc aacatgcaaa aaagccaaag cgtggttaga
    27781 ccagcatggc gttagttatc aaaccgttga tatgatcaaa gaagtaccga aggcctcaac
    27841 gattgagact tggctagaaa cgagtcaagt accaatccgt cgcctgttca acacaagcgg
    27901 gatgaaatat cgggaacttg gactgaaaga tcaagtaggc gagtttacgg cttcaaaagc
    27961 gagccaagtc ttatcgacag atggtatgct gatcaagcga ccactgatct tacgagagga
    28021 ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa ggagaactga tataaaatgg
    28081 caggcaagca tttgaagaaa aaggacaacc tttggatctt atttaatgga aaagaatact
    28141 gtgttggttt gacaaatgaa gcgcaagaag aacttggaga catcacgttt gccaatctgc
    28201 caaaagcagg acaatcgcta aaaaaagatg aacctctgat cgaagttgaa gcggaaaaag
    28261 cggtcaatga atttgccagc ccgctaacgg gtacagtttc ttctgttaat gataaaatcg
    28321 gtgaagatat caatatcttg aatgattcag atgaaatgaa tgcgtggatc ttaagtttca
    28381 aagatgttga tccaaaagaa tttgacgaat tataaaatca gaaaatcact tgaaaaaagt
    28441 tgacactgat tcttccagtt gctataatca taagcaatcc tatatgaaac gagaaagctt
    28501 tgaaaagaag agtacatgtc acggacgttt gaagagagcc ctgtttgctg aaaatgggta
    28561 acgaaaatga gatggaagat ggtcttagag cagagtaagc gagcgtaagc aaacttacta
    28621 cgggagtgcc cgttacagcg cgacagtata cggaaagaag ctaagtccaa gtactggaag
    28681 aggctgttat tgcgaagtaa cggtaaatca aggtggtaac acggaagttc aagctttcgt
    28741 ccttttgctt aaggcaagag ggcgaaggct tttttgtttg aaaaattata agagaaagat
    28801 gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa agacattcaa tgggaaaaac
    28861 ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg aaaaagggga tatttatgga
    28921 atcgtcggct attctggcgc aggaaaaagt acattagtcc gtctcctgaa tggacttgag
    28981 cttcccacga gcggcgaagt gatcgtaaac gatcaagata ttacaaaatt aaaaaacaaa
    29041 gaattacgta cattccgaaa gaaaatcggt atgatcttcc agcatttcaa cctgttatgg
    29101 tcaagaactg tacttgaaaa tatccagctg ccgctggaat tagctggcgt accaaagagc
    29161 aaacgtaaag aacgggcaga agaactgttg cgtttagtcg ggttagaagg acgcggacaa
    29221 gcatatcctt cacaactttc cggcggacag aaacaacgcg tcggtatcgc acgagcattg
    29281 gcaaatgatc ctgagatcct gctttgtgat gaggctacta gcgcattgga cccgcaaaca
    29341 acagaagaag tattagactt attgttagcg atcaataaga aattgaattt gaccatcgtc
    29401 ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca acaaagtcgc tgtgatggag
    29461 ttggggaaag tcgtagaaga aggagacgta ttgactgtct tcagacaacc gaaaaaagaa
    29521 gtaaccaaac gctttgtaca acaagacatc gaaccagaag ctgattcgac agaactatta
    29581 gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa ctttgacctt tagcgaaaga
    29641 aatgccaacg agccagtcat ttcccaagcg attcgacgct ataacatcga tgtcaacgtt
    29701 gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg gttcattgac tgtcttgatg
    29761 actggtgaaa ccaaagaatt agatcaagca gaagtgttca tcaaagaaca aggagtagga
    29821 attgaggtga tccatcgtgg ataaaagctt ttcagaaaca tatctagatt ttaaacaaat
    29881 caacccggaa acgatgaatc aggctatcct tgatacacta tatatgacat taatctcaat
    29941 ggtcttagtg acgatcatcg gattagtttt gggattgatc ctttactcta ttggacgcaa
    30001 aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg atcatcagta atattttccg
    30061 gtcaacgcca tttatgatcc tgatggtttt gatcattcca tttactaaag caatcgtagg
    30121 cactatgcta ggtgcgcaag cagccattcc agctcttgtt ttatccgcag caccatttta
    30181 tgcccgtcta gtagagattg cttttcgtga agtcagccct ggtgtactgg aagcagcaga
    30241 tgcaatggga gcaagttact gggaaatcat ctggaaggtc ttgatacctg aaagtattcc
    30301 cgcattgatt tctggattaa cagttacaac gatctcgatg atcggattta ctgcgatggc
    30361 cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg caggaaggct accagcgtgg
    30421 taatctaaca gtgacattcg ttgcgacttt aattatttta ctaatcgtat ttatcgttca
    30481 aggaatcggt gatttcttaa ctaagaaaac agacaaaaga taataggata tagggggatg
    30541 taggatatga aaaagaaaat ttttggattt gcagcagcgc ttttgctgac tgtaggatta
    30601 gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa ataaagcaga cgacacgaca
    30661 ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt tggaacatgt gaaaccatta
    30721 ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg atgactatgt attaccgaac
    30781 caatctttgg aagaaggcga cattgatgcc aattacttcc agcatattcc ttatttgaac
    30841 aaagaaatca aagaaaaagg atatgacttt gtcaatgccg gtgctgtcca catcgaacca
    30901 atgggcttgt actctaaaaa agtcaaagat gtttccgaat taaaagatgg tgcaacaatc
    30961 attacttcaa attctgaatc agactgggga cgcatcatca caattctaca agatgctggc
    31021 ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa cattcgatga tattgaagaa
    31081 aaccctaaga acttgaagtt caaccatacg atcgacccat cacttttagc ttctacgtac
    31141 caaaatgatg aaggtgacct agttgcaatc aacgcaaact tcgcttacgg cgcagggttg
    31201 aatccattag aagatgccgt attgcttgaa aaggacaact ctccttacgt gaatattatt
    31261 gcgactcgta aaggtgatga agacagtgac aaaatcaaaa aactgattga agtgcttcac
    31321 cgcaaagaag tccaagaatg gatcacagac aaatggggcg gctctgttaa accagtaaag
    31381 gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa taagtctgta atggcttatt
    31441 tctcatcttt tttataccta ttctaattta gaaatcaaaa ttgtttaata ataaataaaa
    31501 gatatttgat tttagtaagg aaggtcattg cgcttcattc tcaattagtc taaaatgagt
    31561 gagaacgaaa gagaatttgg gggtataatg catgtctgtc ttagaaataa aaaatctaca
    31621 tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac ctaacaatga aaaccggaga
    31681 aatccatgcc atcatgggac ctaacggcac aggaaaatct accttatctg ccgctatcat
    31741 gggcaatcca aactatgaag taacagaagg cgaaattttg tttgacggga aaaatgtttt
    31801 agacttggaa gtagatgaac gtgcgcgact tggcttattt ttagctatgc aatatccaag
    31861 tgagatacca ggtatcacga atgcggaatt catgcgtgca gcgatcaatg cgaaacgtga
    31921 tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag ttagatgaaa aaatggcttt
    31981 actaaatatg ccagaagaaa tggctgaacg ttatctgaac gaaggttttt ctggcggaga
    32041 aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa cctacatttg caattttaga
    32101 cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc gttgctaaag gagtaaatga
    32161 aatgcgcgga gacaatttcg gtgcattgat catcacccac tatcaacgat tattgaacta
    32221 tatcacacct gatgtcgttc acatcatgat ggaaggccga gtagtcatga cagggaatgc
    32281 tgacctagct aaacgtctgg aagcagaagg ctacgctggt atcagtaaag aattaggaat
    32341 cgactataaa gaagaagaag cgtaaggggg agcaagaatg aaagaaaaaa actggctcga
    32401 ttatcttgac gcggtaaatg atttttcttt aagtaaaggc gaacctgatt ggatgagaac
    32461 cttccgtcaa gacgcactag cgaaagcaga tgaactgcca ttgccgcata tcgatcgggt
    32521 gaaattccat cgctggtcgt tatttgatgt gaaagaaaca caaaccatca gtgaaacagg
    32581 cacgattccg gcatttgatg caatgaaaga caatcctgtg cttgtccaac aaggttcatg
    32641 gacgattttt gaacaactgc ctgtggaatt agcagaaaaa ggagtcattt ttaccgactt
    32701 gttcactgca atgatcgaat atccagaact agtccaagaa tattatatga aaaaagcagt
    32761 aaacatgaac gaagaccaat tgactgcact ccatgtagct tttatgaaca gcggtatttt
    32821 cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc gagtccttat tcattcaaga
    32881 tggcgcatcc gatgagcact tcttcaagca tgtgttgatc gtggcagatg aacatagtga
    32941 attttcttat ctggaacgtt tccagacaac caaagaacaa gtagcaaaat catcaggtaa
    33001 tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc aaatattcag ccgtagatca
    33061 attaggagaa aatattactt cttatatgaa tcgccgtggg cacattttac gagatgcttc
    33121 tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg atcgctgact ttgactcaga
    33181 tttagcagga gaaggtgcac acgcagaagt caaaatcgtg gcaatcagct cgggcagaca
    33241 aatccaagga atcgatacac gagtaaccaa taaagcccct catacgatcg ggcatatttt
    33301 gcaacacgga gtgatccgag aaaaaggaac attgaccttt aatggtatcg gccacatctt
    33361 gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt gtcttgatgc tttctgataa
    33421 agcacgtggt gatgcgaatc ctattctttt gatcgatgaa aacgaagtaa ctgctggaca
    33481 tgctgccagt gtaggacgag ttgatccaga agaaatgtac tatctgatga gtagaggatt
    33541 gcataaagaa gaagcagagc gcttagtgat tcgaggattc ctcggttctg tcttaacagc
    33601 aatccctgta gaacaagttc ggaaagaatt agtcgaagta atcgaaggga agttgaatgg
    33661 atgatcgatg ctaaaaagct acgcaatgat tttcctatct tatttcaaga cgtcaatgat
    33721 gaacctttag tttatctgga taatgcagcg acgacccaaa agccaaaaca agtactccag
    33781 gcattagcgt actattacga acacgataac gccaatgttc atcgcggtgt ccatacgtta
    33841 gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa aagtacgggc gttcatccat
    33901 gcaaaagaaa cagcagaagt gctgtttact cgtggtacga cgacaagctt gaattgggta
    33961 gccagaacct tcggcgaccg ctatatcaaa gcaggagacg agatagtgct ctcttacatg
    34021 gaacatcatt ctaatgttat cccgtggcag cagttggcaa aacgaacagg tgctgtcttg
    34081 aaatatatcg aactaacaga agaaggcttc ctggatatgg aggatgcagc gaaaaagatt
    34141 acggacaaaa ccaaaatcgt ctccattgct catgtatcca atgtcttagg tgtcttcaat
    34201 cctgttcaag aattagctga acttgtccat gaaaacggcg gggtcatcgt agtagatggt
    34261 gctcaatctg ccccgcacat ggccattgat gtccaagcac tagactgtga tttctttgct
    34321 ttcagcggac acaaaatgtg cggaccaaca ggtattggtg ttttatatgg taaacgaaaa
    34381 tggttagaag aaatggaacc tgttgaattt ggtggcgaaa tgatcgattt tgtcgaatta
    34441 tacgacagta catggaaaga actaccttgg aaattcgaag cagggacacc aaatatcgcc
    34501 ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa aactcggtat ggacaatatc
    34561 catcgatatg aagaagaact agccgcttat gtgttgccaa aattacaagc aattgatgga
    34621 ttgactgttt atggtccaca agacccgaat tttcggacag gagtcgttgc attcaatttg
    34681 gatgggttac atccccatga tgtcgcgaca gctttagaca tggaaggggt agccgtcaga
    34741 gcaggtcatc attgtgcgca accgctattg aagtaccttg aagtatcagc gactgctcgc
    34801 gcgagctttt atttctacaa cacaacgcaa gacgccgacc gtttagttga agcaatcatg
    34861 gcaacaaagg agtttttcca acatgggact atctaaatta gacaatttat accgtcaagt
    34921 gatcttggat cattcttccc atccgcatca tcgtggaagc ttgactgact cgacaagcaa
    34981 gatcgaattg aacaacccca catgtggaga tgtgatcgaa ttgcaagtcg atgtaaaaga
    35041 cggaatcatc aaaaatatcg cctttgacgg cagtggctgc tccatcagta cggcaagtgc
    35101 ctcgatgatg acagacgccg tcatcggaaa atcaatagaa gaagcagaac atttaactgt
    35161 agactttcta cagcttgtcc aaggaaaaga agtcaaagat cccgaccaat taggcgatgc
    35221 tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc aaatgtgcga cacttgcctg
    35281 gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca gctttccatc attaccacaa
    35341 ggaagaggag tgagaacatg ggcgtaccag aattagaaga atataaattc gggtttcacg
    35401 atgatgtcga accggttttc agtacaggag aaggcttgac agaagaagtg atccgtgaga
    35461 tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt ccgtctgaaa tctctagaaa
    35521 catttaacaa aatgccaatg caaacatggg gccccgacct ttcagacatc gatttcgatg
    35581 ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg tgactgggaa gacgtaccag
    35641 aaaaaatcaa agatacattt gaaaagattg gtataccaga agctgaacga gcttatctag
    35701 ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca taatatgaaa gatgaattcg
    35761 aaaagttagg aatcgtcttt actgacactg actctgcttt gaaagaatac cctgaattat
    35821 tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa caaattagca gcgctgaatt
    35881 cagccgtatg gtcaggtggg acctttattt acgtgccaaa aggcgtcaaa gtcgatgttc
    35941 cattacaaac gtacttccgg atcaatgcgg aaaacacggg gcaattcgaa cgtacgctga
    36001 tcattgttga cgaaggagca agcatccact atgtcgaagg ttgtacggca cctacttatt
    36061 caagtaacag cctgcatgcc gccatcgtag aaatctttac aagaaaagat gcgtattgcc
    36121 gttacacaac gatccaaaac tggtcagaca atgtgtacaa cttagtaacc aaacgagcaa
    36181 aagcatacga aggcgcaacc gttgaatgga tcgacggaaa cctaggtgca aaaacaacga
    36241 tgaaataccc aagtgtttac ttagatggaa aaggtgcacg aggcaccatg ctctccatcg
    36301 cctttgcagg agcaaaccaa atccaagata cgggtgcaaa aatgatccac aatgcaccaa
    36361 atacatctag ctctatcgtc tctaaatcaa tttcaaaaga cggcggagaa gtaaactatc
    36421 gaggacaagt aacctttgcc aaaaacagtg cgggttcgat ctctcatatc gaatgtgaca
    36481 cgatcatcat ggacgaatgg tcgaaatccg atacgatccc attcaatgag atccacaaca
    36541 gccaagtctc tttagaacac gaagccaaag tctcaaaaat ctcagaagaa caactctact
    36601 atctgatgag ccgaggcctg accgaatctg aagcaactga aatgatcgtc atgggctttg
    36661 ttgaaccatt caccaaagaa cttccaatgg aatatgcagt cgaactcaat cgattgatca
    36721 gttatgagat ggaaggcagt gtaggttaaa cctgttatag gagctagaac ccttgatata
    36781 taaggattct agcttttttt gttttgcctg tatactagaa atgaccagct tttgaccagc
    36841 tcttttactg atgagccatc atatcagcaa agtcttgacc tgttttctta ggtgcattat
    36901 ttgtaatatg tgcatagatg tctaatgtca ctttactatt ttcatgccct aatcgttgtt
    36961 gaactacctt gacagattct cctgcttcta ataacaaact agcatgtgta tgtctaaacc
    37021 catgaggtgt aatacgaggc aagttatatt ttttcaaaat atatcttaac caatcattaa
    37081 cagtgtttgg aatgtataga gtgtttttaa ctgttgtaaa aacatgttgc tctttagagc
    37141 tagtattgta tccaagtttt aaatactctt ctttttgctg taatttccag ttgttgagga
    37201 tagaaagagt ctcagtatct aaagagataa ctcgtctaga agctcttgtt ttcggagatt
    37261 gaattataac gttaccaaat tcatctacag caagagtttt attgacatgt agttctttat
    37321 taaacgtatc tatgtctttc cactgtaaag cgagtacttc actctttctc atacctgtgt
    37381 atgccaataa tctgaagaag gcgtattgtt tcatgttacc aaaatcttta aaagcatcca
    37441 gtaaatcatt aagctcctct tttgagtaaa atttacgatt tttctcatac tctttttttc
    37501 gtggaagaag tgttttttcc ataggattat tgtcaatata ttgcattgca accccgtagc
    37561 gtaaaatttg tgcagtctgt tttctcatat agtcgtaact ttcatattta ctgtgccatt
    37621 catctaccac tttttgacaa tatgggacag agatattcga taatttaagc ttaccaaatg
    37681 ctggaagtat ttgattattt gcgtatctta ctgaaattgc gattgttgaa gctttaacac
    37741 tggtacgctg atgagctatc cattcattat acaggtcttc aaatgtcata tcaggtgctt
    37801 tctttttaac accgtataat atttgatgtc tgtattcgtc ataatctttt tgagcttctt
    37861 gtttgctact aaaacctctt ctcgtagtat ttattttttc accagttcgt ggatcggtgg
    37921 caatgtgtcc tttatacatc cacgccactt gaccgttttt ctttttgtac tttgatattt
    37981 ttgccatgaa atcatctcct cttagtttac aaggagtact taccactaga tggtatcacc
    38041 tcccttcatt tttttcttag tgtatttttt cccaattgat gaaatcgttt gttctagctt
    38101 gtttatatta tcagtaattt ctctcacagc ttcttcggga taagacatgt ctttcaattg
    38161 aataggtagc cattcatcta ccattctttc tagtagttct tctatactgc tagtgaggaa
    38221 aagaatgtta ttgtcaaatt cttctacaat agcattgatg aataattctt ctatttttaa
    38281 gatattgaaa agactaattt tttcattttt aactttatta caagtcttgt tagcaataaa
    38341 ttcttgcatc tctggttcta atttggaata tctatctagc catgtatcta aattagagtg
    38401 tactggtggg aactgttgac gaagatgctg gaacaggttt ttatctggaa atgttatacc
    38461 atctagtaca atgctatctc tataaagaat aattcccaaa atatattctt ctacattgcc
    38521 acaaactaac tcctctatac tcatatttct caaatcagca attcgggata atcgctctgg
    38581 tattggtaaa taggttccct tttcccattc gtagatagtt tttttgtcaa tcaattgacc
    38641 gttgttaatc ataagaccaa atgctggttg tgttaatttt ttactctttc ttatagcttt
    38701 tatttttgtt ccgagtatct tttttcgttc taaataattt attggtggac gctttatcat
    38761 attgaccaca cctcctaaaa ttagtatacc ttaaaaaagg ggtgttgtaa ataaatttaa
    38821 caaaaatatt gacacccctt tttcgtcgag gtattatgtg tatgtcgatg aactggtaat
    38881 cattttttgt ttgattaatc ggttaataac aaaatacttt tattttgagc aaaaaatacg
    38941 aagatgattc ttagaaaatt tgttcaagca atcacgaaag gaggggaaaa tatgtctcta
    39001 caaatcattg atagcattgt ttctatacaa gttgaacaac aagtgaaaga gataattaca
    39061 gtgctaaagc ctatgctaga agacattatt tctggagtca ctctagagta tatggactat
    39121 cagcaggcaa gtgaatatct aggtgtatca gtaggaacga ttagaaaata tgtttctcaa
    39181 tacgggttac ctgttataaa aattgatacg gtcattagat tcaaaaaatc tgatattgat
    39241 agtttcatgg aacagtataa aggttgagta aacaaataag actaaaacat atagtaataa
    39301 agagtttgtg tttattatct gtatgtttta gtctttaaca gaattttata aggaggaaac
    39361 acaaatggaa acaccaatga ttaatattcc aattaatgta gataaagaaa gactattaga
    39421 gaaaccagtt atagaggatg aaaaaaagag aatacgaaaa agatgtttag ataactacag
    39481 agaagttaca agagatgagt ttgtagagat actaaattca tcacaatcat ttattccaag
    39541 taagacaaag aataaaggca acaaaagtga agagttttta gaaactagag ttattattct
    39601 ggatgtcgat aacactgtta aagatgagaa ggggaaggta gtagatttaa gtaaagatga
    39661 tagtagatac ttaagcatag aaaaggcttt atctatcaat ttagttcata attctgcgtt
    39721 tgcgatacaa aaaagtattc ggtactctga aaatctagaa aaattcaaga ttgtctttct
    39781 attaaaagaa gcaattactg actatagtaa aatggttgct gtttacgaat acttgaaaaa
    39841 aatcataccc gggtgtgatg acaatgtaaa tgcttcgaat agaatgtttt ttggaggata
    39901 taagtcagat gcagtgataa tgattaatga agacaacatg ttagatatta ctgagcttcc
    39961 gattgatttt accatgacat gttctaaaaa ttgtcaaagt gattcaggga gagagtatga
    40021 attagtcaat gaaactgatt ttgttaagtt gataaaaaat gacgacaaag aagaaatgaa
    40081 acagtggttt aaagactgct tgtttgattc ctccgacatg tctttcaatg agatttatga
    40141 aagattgtta accattgata tggataaatt acttaagtca agtaaaaact taagatgttt
    40201 gtttcacgac gaccataatc cctctgctag tatttttacc agtaaaactg ggcattccat
    40261 tttttattgt cattccagta attgtagtat cagtgaaaat tttattgggg ttgtgatgtt
    40321 aattctaaag aaagacagtc gggtagaaac atttaattgg ctattagaga acctagacct
    40381 gtatcctaca cattttcgaa aactgaaaga ggaatcaaaa gtactttttg acactcttga
    40441 atcgatggaa gacaagcttt tcaggacaat aaggaattat ctagaggaaa tgagacatgt
    40501 ctatgatatc ttgctttctg atgttaattt ttttgatgat agtaaggaaa gtgtgacttg
    40561 tattttgtcg ggggaacaat tagctaaaaa aatgagttct tactatggta aaacttatga
    40621 catagataaa tgtaataagt tactatcact tatgactttt ataggattga taaaaaaatt
    40681 agatgatgaa gatgttcctt tgaagatgct cgaataccta aataggttaa aggaagagaa
    40741 agcgttagga aactttaatc ataagagaag caatgtctat cgattagacg tttttaatgc
    40801 aacttctgtg attcaaaaat tgaatgaaga tatccttcca cttctggaaa aaattcattt
    40861 tacatatcaa tatttttctt atgaatgggt taagatatgt tttaacgaaa aagaggctaa
    40921 aagagtattt cctcaaaatt cccgatcaga actcagcaag gaaaaacaat tgattttaga
    40981 tgaagcacat gaagttttac agggcttatc gtggcaaggg acatatctaa tgacagagaa
    41041 agaacttatt aacactgttt gtgaacgcct acgtaatgtt ggggataaca gcgtgaaaaa
    41101 tgctttaata aaaaacagag gctactttgt taaacaagga ttcactcttg aacgagcttc
    41161 taaggatatt aagaaactat acaatgtgaa agcaagtagt agttttttaa tatatttagc
    41221 gaaaggggca gggatagatg aaacaataaa agaattgata ttatcaggag ctggggtagt
    41281 tcctctgtat caatttacaa gtgtaacaag agatgatgga cggagagtaa ggaagagaaa
    41341 agacccaatt gtattaaact agacaggtta tccatagctc ttaatcttat ttcatctcaa
    41401 tacacccacc gttaaagttc ggtgggttat ttatttttca atctttcaat agctgattgc
    41461 gttcattttc ttgccttttt aatagcttca tatagtcaat tgttcgaact gcatcttcag
    41521 tgctaagaga acgatagaga gccaaaaact cttcctcata atctacgaca acattgtcta
    41581 gaaaatggct tacaggggtt cctagggctc tagaaagagc tgttaagcta tcgaggttag
    41641 gttctgttac agaattttcc catcttgcta ctgcctgttg tgtagagaag atttttttag
    41701 ctaattcttt ttgtgtccac ccgagagctt ttcgcttttc tctaatgacc tttgcatatt
    41761 gtttttgttt cattaataca cccccttctc agtagagtac aacaagttgt tgtatttgta
    41821 atgatatttg aatttttaat atgttttatg agttgaaata catcatattg ttgtatataa
    41881 tggaacagga ggggagaatt atgaaaaaag taagtaatct cagccgatac agaaaagaaa
    41941 aaggattttc tcaaactgag ttagctaaaa aaatgaatgt tacacagcaa tgtgtaagtt
    42001 cgtggcaaac agggagaacg attcctaaac cctatcaaat gaaaatgctt tctgagatat
    42061 taagtgtacc aataaatgag ctgttttctg atgtatttaa taaagtaaac agttaactaa
    42121 taaacaattg aatgttttta ggaggagaag acatgtattg tagaaaatgt ggcaaagaaa
    42181 taacagatga aagtaagtat tgccagtttt gcggagcact acaagaaaca gatgagacaa
    42241 ctggagcaga cgcagtaaag aaaagtactg agcagacaat taatgaaatg aaggaacaaa
    42301 tcgtacatgg acaagagaca gggaagctgt atttgattat tggttggatt tctatggtag
    42361 tatcgttagc atttattcct gttgtatttg gtgctgttgc tgttattatg ggttacttat
    42421 atcgagagaa agatgaaaaa atgggtacga ttttgatgat agcaggggta gctggagcca
    42481 tctttggtgt acttttaggg atgtcaatgt actgataaga gggataagag caaattattt
    42541 tggagatagg agataaaaag ttgaataaag taaatatgaa caagaagttt gtcattatag
    42601 gtattgttgt cttggctatt ataggtatca ttttttcaaa aatgacaggt acatctggat
    42661 tagagaagtc atcgaaacca ttggtaacac agattttaca agaacagtac agtttaaatc
    42721 ggacatgtga tgatgtgacg attactcatg aaaatggaga taacacatat cgagctaaag
    42781 ctatattaga caatggaagt gctatcaaca taaatattga gtactatcca aagaaagacc
    42841 gtatatatgt agagatacca tatactgagg tgttaatgtt gaattgaacc ccaccccatt
    42901 gatgattgac cgtattttgc atggaaacct tgggaggcat aagctaacta ggggttcctt
    42961 tttcttttct ttttttggaa tctatttaag tgaaatgtat ctgaaatgaa atgggagtaa
    43021 gtgttataat agttaataca taagcgcttt attttatttc agatggtgaa tagtataagg
    43081 aatttttttg atattattac agtttatctt actataaagt actatttttt aaagcaataa
    43141 cagatgaaaa tttgattcat tcataacaac atgtacagct ataccaccta cttggaataa
    43201 ctgaagaaaa tgtacgaaaa accatttcaa gtaaacccca aatgattgtt aatgaactac
    43261 aagggtggtg aagcttcatg agtctccaca gagatgctcc atcaaaagtt ccgtcaagct
    43321 catgaaaatt gtgaaaaacg ttttgaataa tatcaaagaa agagggaatg gaaatggcac
    43381 acaatgaacg ttttaaacaa gaaattattc aagaactcga gaaaggagta caacaccaaa
    43441 aattaatatc aaagaccgcc caaaaatttg atatctcact ggctaaggag gttaaacttc
    43501 atgaagttta acctgctagc tctactaacc gtggtaacgc tcgtttttgt tggttgtact
    43561 aaggctcctg attccttgaa aaattcttct aacagtgcgg aggtaaacgt ttcgacctct
    43621 accagtgaag cggtacagtg ttcacaagaa actactagct tgccagaaga gctacttgga
    43681 gattggcaga ttaataataa ggatgctggt atagagatat cagacagtaa agctgtattc
    43741 cgtataggaa ggtctcaagt agagtcaaag gactattatg tagatgtaaa caacaaaatg
    43801 cttttacatg tctatttttc ttttgagtat gatggtgtga aatacagtga agtctctatt
    43861 gaaagaactg gctatagtga atttattagc aaaagcgtgg gaattttaac tatgcatact
    43921 aaagatggac aatatataag tttgggtgtg aagaaagttt ccggtgagaa taatgagaca
    43981 gaagcagtta cggagcagac gatggatgaa agaattgatt catctgagag taatgagaca
    44041 gaagtagata ctgggaagac gatagatgaa agaattgatt cattcatcga aacatacaca
    44101 gctataccac ctacttggaa acttgtaaat gaagataaag gaatcacgat aaatactgat
    44161 acagagattt ctacagatgc tcaatcttta tcagatttat tagccaacct aacaggaatc
    44221 agtaatgcgg tgacagagca agttggtaaa aatgttccac ttcggttact tgaaaatggg
    44281 aaaaaagatt attctgtggt atttaagaac ggacaggtaa ctgattacaa cgaagtattt
    44341 aaaaaagtta ttgaataatg cttcttcaat ctttttcatt catttacccc accccgttgt
    44401 tatataatcg tatctcaaga ggaaccatta gaaagcatag gctaactagg ggttcctttt
    44461 tcttttgttt ttttggaatc cgcttaagtg aaatgtatct gaaatgaaac gaaaataacg
    44521 tgatttttta ctgtaatatc aaggttttag ctgttttaga taccatgtaa cccaaaatag
    44581 tttattgaga aaataggaga gtatgtgata ctaactagta gggaaaaatg agataaaaag
    44641 taaatggaat catttcagaa ataagtgaaa taagacaacg taataggtat aacatgacta
    44701 ttacatagtt ttagacatga aatattacta caaaatttgt ctaattatgt cattatttat
    44761 ggaggagttt tataacgatt tttgtaataa aaagacgaga aggaatgcat acatgcacat
    44821 cctctcgtct tttaactatt tactttagga attacaacaa ttgctgtaat tcgtcaccta
    44881 ttcgttcaac tattttcgtg actaaagcat ttcccataaa gaataagcgc attctatctg
    44941 aaacttctac tatttccccg gaggataatt ttttatactt tgtccaatca tcaggaaagt
    45001 cttgtagacg ttcggcttct ataggggtca acaagcggta acgaccatct atttttaata
    45061 aatgtgtaga tctattcaca gaaccttctg aagttagcat cgtccgtccg ggcatatcta
    45121 aatcatcata aggagacata ccaccttcgg agaatgtata ggtatgacct tcagcagtga
    45181 ctcgctctat ttttttaggt ccacgaagat attcgaattt ttttatttta ctttcgtcgg
    45241 tcaagtaata cttttcagct acttcatctt caggttgaat gatttctctt aatggagtag
    45301 gtttttcttt atcgtatggt tccgtatcta tagtaaagta acgtccatgt ctcattaccc
    45361 cagtattgta tacttttcca gtaaaattat ctgatatctc tactatgtca ttggataact
    45421 tatacgacgc ttgtctaccc ttataaggag tcttgatgat tggaaattgt cgagcaaaaa
    45481 gtccttcact gaatatatac tcatcataac gatgttcttc ttcaccaagt attttttgtt
    45541 cgaatcgtgc atctatcttg ttcgcaaaac cagtatcatt tttgtaaacg aaaaaaaaga
    45601 cacggcgtct acgttgtcta cggccataat cagcagcatt gataacacgc cattctacgg
    45661 aataccctaa atcattaaag gaagcaagca ttatagcaaa atctctgcca cgttgtttag
    45721 aaggagattt aagtagacga tcaacattct ctaaaatgag atactttggt tttatgtggt
    45781 tcgtagcacg gattatttgc caaaatagaa ctcctttttt accttctatc cccatctcat
    45841 gttttttact acgtgcaact gaataatctt ggcacggaaa tccaccaaca atgatgtcag
    45901 cgtccatttg ctcaaattct tcatttgata tttcttctat attcctgttg atattttcac
    45961 tatttgggaa tcggtagtca tatacttcaa aagcatcttg tgattttcga gaaggctccc
    46021 attggtttgc ccatttcgtt ttgaataggt cggcattaga atgctcgagt ccaactctga
    46081 acccccctac accggcaaaa agttctaata catttagtgt ttcactcata ttggcgaaca
    46141 tcctttcggt tttaacttaa atgtattata caataaataa atcgatttga aaagatcgaa
    46201 atcaaaaatg ctatgattta cttaaggagt ttctcaaggt tattctgtaa agttgaaaca
    46261 acatcaaatg attttgtata gacttggttt ctatgtaacc atgtagtata gttttatata
    46321 ggaataaacg taggatggag tgactgaacg aatgtctgaa tacttaacaa aagagcaagt
    46381 acataataga gctaaagaag cagtggggaa gtctatgata gaattaaata atggaattcc
    46441 ccttctcaat agtaaaaata aaaactctat aggagacata tttgagtctt ggttcggcaa
    46501 ggtaaaggat agcgagagta ggccagacct ggaagaagca ggtgtagagt taaaggctac
    46561 gccaataaaa aagttgaaaa acggtcagta tagcgctaaa gaacgattag tgttgaatat
    46621 catcaactat aatgaggtcg ctgaggaaac attcgaagaa agtcattttt tgtataaaaa
    46681 caacatcatg gaaatcgcat tttatgaata cttaaaagat acgccgaaag accagtggac
    46741 tatccgtgaa gctattttat atgagatgta taaaaaccct gtagattttg aaatcatacg
    46801 acaagactgg gagcttataa agaaatatat tttagatggg aaagctcatg agctaagtga
    46861 aagtttgacg aactatcttg ctccatgtac aaaaggaact aatgctgtga agtcactcag
    46921 gacacagccc tattcagaac aaaaagctaa acaacgggca tactcattga aatcaggata
    46981 catgacatct attttacgta aatatgtatt aggcgatgaa aaaatcgatt caatcgtgaa
    47041 gaatactttt caacttgaag aaaacaactt agaagattta gtattagaac attttatacc
    47101 atatgtaggt caaactgtac aatctttatg tgaaaaattc acaatcaaga catcgtatca
    47161 agctaattat agaatcgttt cttcaatact agggttatct ggtactgtaa ccaaagggga
    47221 acctttccca agagtcgaag aatttgaaaa agcgtctata ctagttaaaa ccatatattt
    47281 tgataaaagt ggaaataaca aagaaagcat gtcatttcca tcattcaatt ttattgattt
    47341 gagcaaacag gtatgggaaa aagaagatgg ggaaccaagc agtgattggc ataatttttt
    47401 attagataca agatttcttt ttgtagtctt taaagaagaa gatgaaggtg ttgtattcaa
    47461 gggagctaaa ttttttagta tgccagaaga agatatagaa gggccagtcc gtaaggtatg
    47521 ggaagatact gtgtctaaac ttcgagaagg agtagagtta gaagctattc ctgataaatc
    47581 tactaaggat ggatggaaaa tcaaaaataa ttttataaag aaaacagaca aaatgatttg
    47641 ccatgttaga ccccatgcta gccaacgaga ttactctgtc gatggacgct atgcggataa
    47701 acttcctgta cccgcaaaat ggattaataa accagacaac gataattact cggatcaatg
    47761 gatgaccaaa caatgttttt ggataaacaa tgattatata aaagagcaag tacaagattt
    47821 gatatcataa aaaaagagga agactactcc tctttttttt gtgcaatata atctaatatc
    47881 acagatgtgc aataatttaa gtttttcaaa atatcttttt cccaaaagtg tataggtacc
    47941 caaccaagtt ttatgagaga ctgattattc ctttcatctc tcctcatatt ttcttctatt
    48001 ttttcaatcc agtagtcccg attccgtttc aacttttgtt ttctattgtc ccaatcgtgt
    48061 ccgtgccaaa actctccatc gacgaaaatt gctagtttat atttggtaac tgctatatcg
    48121 ggagagcctg gtaatttttt atagtttttt cgatacctga caccttgatg ccataactct
    48181 ttggctagca gactttcagc ttttcctccc ttcaagcgta catttgccat acgttttgat
    48241 acttcgggag tggtcgatag ttgtctatgc ttcattgttt tttacctcca atgttctaat
    48301 agaatcaaga aatgttagta cagagatatg atttatttaa gatatcacta tcataaatat
    48361 atagctaaat gaaaaaagtt gcgaatatgg tcttatagaa atagtaatta ttttcagttc
    48421 tcagcggtct tattgattcc gttttattga ttttaaatgt tattcagtga ccctttaaca
    48481 ggtcaatttt tatgtttttg tcttcgtatg actcagtttg atatctagtg atatagtgat
    48541 ttgagtctac atcgaacaat ggaataatcc aataaaaaat gatttagaat atcaaaataa
    48601 caaaaaagag gactacctgt attagtcctc tttttgacca gattt
//
LOCUS       DAFCNS010000012        43679 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFCNS010000012 DAFCNS010000000
VERSION     DAFCNS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.012"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..629)
                     /locus_tag="LNE23_000671"
     CDS             complement(105..629)
                     /locus_tag="LNE23_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6930547.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="LNE23_000672"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="LNE23_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBK6930548.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="LNE23_000673"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="LNE23_000673"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBK6930549.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="LNE23_000674"
     CDS             complement(2351..3031)
                     /locus_tag="LNE23_000674"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK6930550.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="LNE23_000675"
     CDS             3157..4026
                     /locus_tag="LNE23_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6930551.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="LNE23_000676"
     CDS             4052..4567
                     /locus_tag="LNE23_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930552.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="LNE23_000677"
     CDS             complement(4614..5207)
                     /locus_tag="LNE23_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6930553.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="LNE23_000678"
     CDS             complement(5232..5411)
                     /locus_tag="LNE23_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930554.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="LNE23_000679"
     CDS             complement(5488..5997)
                     /locus_tag="LNE23_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930555.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="LNE23_000680"
     CDS             complement(6093..6473)
                     /locus_tag="LNE23_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6930556.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="LNE23_000681"
     CDS             6803..7552
                     /locus_tag="LNE23_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBK6930557.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="LNE23_000682"
     CDS             complement(7736..9013)
                     /locus_tag="LNE23_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930558.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="LNE23_000683"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="LNE23_000683"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBK6930559.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="LNE23_000684"
     CDS             complement(9935..10879)
                     /locus_tag="LNE23_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBK6930560.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="LNE23_000685"
     CDS             complement(10881..11567)
                     /locus_tag="LNE23_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBK6930561.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="LNE23_000686"
     CDS             12041..12361
                     /locus_tag="LNE23_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBK6930562.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="LNE23_000687"
     CDS             12515..13420
                     /locus_tag="LNE23_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6930563.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="LNE23_000688"
     CDS             13417..14190
                     /locus_tag="LNE23_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK6930564.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="LNE23_000689"
     CDS             complement(14371..15795)
                     /locus_tag="LNE23_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6930565.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="LNE23_000690"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="LNE23_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK6930566.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="LNE23_000691"
     CDS             complement(16517..18499)
                     /locus_tag="LNE23_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6930567.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="LNE23_000692"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="LNE23_000692"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK6930568.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="LNE23_000693"
     CDS             complement(19412..20473)
                     /locus_tag="LNE23_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK6930569.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21250)
                     /locus_tag="LNE23_000694"
     CDS             complement(20684..21250)
                     /locus_tag="LNE23_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6930570.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="LNE23_000695"
     CDS             complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="LNE23_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK6930571.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="LNE23_000696"
     CDS             complement(23038..24159)
                     /locus_tag="LNE23_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK6930572.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="LNE23_000697"
     CDS             24285..25226
                     /locus_tag="LNE23_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK6930573.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="LNE23_000698"
     CDS             complement(25376..26272)
                     /locus_tag="LNE23_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBK6930574.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="LNE23_000699"
     CDS             26541..27881
                     /locus_tag="LNE23_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6930575.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="LNE23_000700"
     CDS             28099..28542
                     /locus_tag="LNE23_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBK6930576.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="LNE23_000701"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="LNE23_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBK6930577.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="LNE23_000702"
     CDS             complement(30867..32090)
                     /locus_tag="LNE23_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBK6930578.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFLYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="LNE23_000703"
     CDS             complement(32121..32960)
                     /locus_tag="LNE23_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK6930579.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="LNE23_000704"
     CDS             complement(32977..33657)
                     /locus_tag="LNE23_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6930580.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="LNE23_000705"
     CDS             complement(33659..34732)
                     /locus_tag="LNE23_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6930581.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(34788..35026)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(35069..35302)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35379..36050)
                     /locus_tag="LNE23_000706"
     CDS             complement(35379..36050)
                     /locus_tag="LNE23_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6930582.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="LNE23_000707"
     CDS             complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="LNE23_000707"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBK6930583.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="LNE23_000708"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="LNE23_000708"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBK6930584.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40799)
                     /locus_tag="LNE23_000709"
     CDS             complement(39933..40799)
                     /locus_tag="LNE23_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6930585.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40959..43052)
                     /locus_tag="LNE23_000710"
     CDS             complement(40959..43052)
                     /locus_tag="LNE23_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK6930586.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        12612 a         9150 c         7716 g        14201 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aaagaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctctaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc ctccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    40801 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    40861 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    40921 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    40981 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    41041 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    41101 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    41161 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    41221 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    41281 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    41341 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    41401 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    41461 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    41521 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    41581 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    41641 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    41701 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    41761 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    41821 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    41881 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    41941 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    42001 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    42061 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    42121 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    42181 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    42241 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    42301 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    42361 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    42421 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    42481 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    42541 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    42601 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    42661 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    42721 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    42781 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    42841 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    42901 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    42961 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    43021 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    43081 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    43141 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    43201 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    43261 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    43321 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    43381 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    43441 gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    43501 cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg
    43561 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
    43621 ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgata
//
LOCUS       DAFCNS010000013        43405 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFCNS010000013 DAFCNS010000000
VERSION     DAFCNS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.013"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..210)
                     /locus_tag="LNE23_000711"
     CDS             complement(<1..210)
                     /locus_tag="LNE23_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK6930587.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..623)
                     /locus_tag="LNE23_000712"
     CDS             complement(309..623)
                     /locus_tag="LNE23_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930588.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(691..1185)
                     /locus_tag="LNE23_000713"
     CDS             complement(691..1185)
                     /locus_tag="LNE23_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930589.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1205..1492)
                     /locus_tag="LNE23_000714"
     CDS             complement(1205..1492)
                     /locus_tag="LNE23_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930590.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNK"
     gene            complement(1708..3099)
                     /locus_tag="LNE23_000715"
     CDS             complement(1708..3099)
                     /locus_tag="LNE23_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK6930591.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3084..3695)
                     /gene="mobC"
                     /locus_tag="LNE23_000716"
     CDS             complement(3084..3695)
                     /gene="mobC"
                     /locus_tag="LNE23_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBK6930592.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(3699..3896)
                     /locus_tag="LNE23_000717"
     CDS             complement(3699..3896)
                     /locus_tag="LNE23_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930593.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            complement(3887..6565)
                     /locus_tag="LNE23_000718"
     CDS             complement(3887..6565)
                     /locus_tag="LNE23_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930594.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            complement(6552..6770)
                     /locus_tag="LNE23_000719"
     CDS             complement(6552..6770)
                     /locus_tag="LNE23_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930595.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(6846..7898)
                     /locus_tag="LNE23_000720"
     CDS             complement(6846..7898)
                     /locus_tag="LNE23_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK6930596.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(8051..8377)
                     /locus_tag="LNE23_000721"
     CDS             complement(8051..8377)
                     /locus_tag="LNE23_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930597.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(8389..8589)
                     /locus_tag="LNE23_000722"
     CDS             complement(8389..8589)
                     /locus_tag="LNE23_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930598.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            8712..9314
                     /locus_tag="LNE23_000723"
     CDS             8712..9314
                     /locus_tag="LNE23_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6930599.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            9369..10535
                     /locus_tag="LNE23_000724"
     CDS             9369..10535
                     /locus_tag="LNE23_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6930600.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(10624..11016)
                     /gene="rpsI"
                     /locus_tag="LNE23_000725"
     CDS             complement(10624..11016)
                     /gene="rpsI"
                     /locus_tag="LNE23_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK6930601.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(11032..11475)
                     /gene="rplM"
                     /locus_tag="LNE23_000726"
     CDS             complement(11032..11475)
                     /gene="rplM"
                     /locus_tag="LNE23_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBK6930602.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(11994..12461)
                     /locus_tag="LNE23_000727"
     CDS             complement(11994..12461)
                     /locus_tag="LNE23_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBK6930603.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(12581..14119)
                     /locus_tag="LNE23_000728"
     CDS             complement(12581..14119)
                     /locus_tag="LNE23_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBK6930604.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            14199..14885
                     /locus_tag="LNE23_000729"
     CDS             14199..14885
                     /locus_tag="LNE23_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBK6930605.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(15086..15613)
                     /gene="msrA"
                     /locus_tag="LNE23_000730"
     CDS             complement(15086..15613)
                     /gene="msrA"
                     /locus_tag="LNE23_000730"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK6930606.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(15697..16374)
                     /gene="pgmB"
                     /locus_tag="LNE23_000731"
     CDS             complement(15697..16374)
                     /gene="pgmB"
                     /locus_tag="LNE23_000731"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK6930607.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(16371..17501)
                     /locus_tag="LNE23_000732"
     CDS             complement(16371..17501)
                     /locus_tag="LNE23_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK6930608.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(17501..19684)
                     /locus_tag="LNE23_000733"
     CDS             complement(17501..19684)
                     /locus_tag="LNE23_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK6930609.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(19681..20706)
                     /locus_tag="LNE23_000734"
     CDS             complement(19681..20706)
                     /locus_tag="LNE23_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6930610.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(20751..21578)
                     /locus_tag="LNE23_000735"
     CDS             complement(20751..21578)
                     /locus_tag="LNE23_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK6930611.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(21575..22642)
                     /locus_tag="LNE23_000736"
     CDS             complement(21575..22642)
                     /locus_tag="LNE23_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBK6930612.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(22668..23504)
                     /locus_tag="LNE23_000737"
     CDS             complement(22668..23504)
                     /locus_tag="LNE23_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6930613.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(23501..24370)
                     /locus_tag="LNE23_000738"
     CDS             complement(23501..24370)
                     /locus_tag="LNE23_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6930614.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     SVVLLSRKKVDLK"
     gene            complement(24440..25723)
                     /locus_tag="LNE23_000739"
     CDS             complement(24440..25723)
                     /locus_tag="LNE23_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK6930615.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(25737..27422)
                     /locus_tag="LNE23_000740"
     CDS             complement(25737..27422)
                     /locus_tag="LNE23_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBK6930616.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            27697..28722
                     /locus_tag="LNE23_000741"
     CDS             27697..28722
                     /locus_tag="LNE23_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6930617.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(29317..32991)
                     /gene="rpoC"
                     /locus_tag="LNE23_000742"
     CDS             complement(29317..32991)
                     /gene="rpoC"
                     /locus_tag="LNE23_000742"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBK6930618.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(33049..36666)
                     /gene="rpoB"
                     /locus_tag="LNE23_000743"
     CDS             complement(33049..36666)
                     /gene="rpoB"
                     /locus_tag="LNE23_000743"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK6930619.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            37394..38362
                     /locus_tag="LNE23_000744"
     CDS             37394..38362
                     /locus_tag="LNE23_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6930620.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            38380..39285
                     /locus_tag="LNE23_000745"
     CDS             38380..39285
                     /locus_tag="LNE23_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6930621.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            39282..40088
                     /locus_tag="LNE23_000746"
     CDS             39282..40088
                     /locus_tag="LNE23_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6930622.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            40224..41213
                     /locus_tag="LNE23_000747"
     CDS             40224..41213
                     /locus_tag="LNE23_000747"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBK6930623.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(41530..42054)
                     /locus_tag="LNE23_000748"
     CDS             complement(41530..42054)
                     /locus_tag="LNE23_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBK6930624.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            42341..>43405
                     /gene="scm"
                     /locus_tag="LNE23_000749"
     CDS             42341..>43405
                     /gene="scm"
                     /locus_tag="LNE23_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBK6930625.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        12445 a         9024 c         7631 g        14305 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct tccttattta tcactaatgt ttcaaacgga
      601 acaactttat tttcctcagc cattctccca gttccatttt caaaaatcac aaaactgata
      661 ttaccttaaa aactactaac aaaataaaac ttataaagta aatggcaacc caactatgta
      721 accattctct ctacgtacta cgatttggta gtcctgcctt tcaatcggta attcataata
      781 gtaatttact gtttcttctt tgccactttc aatatactct ggattatcca atatcatatc
      841 atactcataa agttcttcac catccttatt ttcaatcgac cattgttgtg gacgtatcgg
      901 attttctgta tcatttataa gtgtataggt cacttgtaat acacgatcat gttgtgcaaa
      961 ttcatttctt ttttcggtca cttcaatatt tttaaatgtt agttcaaccc catctgcctg
     1021 ataagtttca cctattttta aatcttttgt tacaattttt attggctttt cttctggaaa
     1081 atgtctggct ttaattgtta gataacttcc aaaaagaatc aatcctagta gcactaaaag
     1141 aattccttta acgagccact gaaacgggaa gtaaacttta aacatctttt ttctcctccg
     1201 ttttttattt gttttttaga gaatacgata acttttgatc aatgtcatcc agcttttcaa
     1261 ttacttcatt acttatcatt gattctagtt ctgaaatttt ttgttcaaat ttaatgcttt
     1321 caggatcatt aaatttatta tttgatggac tagctaacac tggtaaattc gaaaccataa
     1381 cacgaaacat atttgccttt gaaccaaaaa ctcctttgtt ttggttgtaa aaattgttga
     1441 atatttcttt agtttcttct gaagccgaga ctttaaaata tggatagttc atttttttct
     1501 tccacctttc tttttttggg agggagtctg tagggagctt gctccctcag agatttaccc
     1561 accatctgac ttgccagaaa gtagcctagc gtgagcgggg cggattgact ggcaagtaga
     1621 tgggtagctg gcgtatgtcc taaaaactgc ttctttccaa gcaactcttc ataggcaaaa
     1681 aactggaaag tccactaaca agcccattca gaattccatt tcaaaatttt gttgcctttt
     1741 ttgctgattt tcaatcattt gttgtgtgaa ttcataatcc tttttagcac gatacatttt
     1801 tacatcgtca ttcattatgc gactgatttg atagtgaatc tgtttagcag aaggtgtatg
     1861 agaacaattt tcaaaaccct ttgccggtag aaataactct ttctgatcag tctttttaat
     1921 agaaattgtt ttacttttcg cactacgtaa agtttgagga ttcacatccg tatgaagttt
     1981 aatcgtgtat tcttgatcat cttctaaaga cattgtattt acaaaatgct tgtctacctc
     2041 tcgcaagaag tcatcatatt taatgaattt ttcatcgcct tcacggtcgc taaattgctt
     2101 ttgtgcgatg gaatctaatt ccacaaacgg tacatttaac tcaccagttt tgtaatttac
     2161 tccactaatt agcgacataa cttgctctct ttccatatta aaaaaatttt ttatatgtga
     2221 ttcttctaaa ttcttttcaa gttgttcaga aacagattca aatgaatatc ttttccctaa
     2281 ctttcttgca ctaaaagtac gttcgacata ctgacttttt tcttttctag attgtttatc
     2341 tcttcctaaa gtaattaact cacctctacg ctcatagaaa tttacacctt gtttgtaaag
     2401 aacatagcca agtttgttat ttgtttttga aaattgataa acttcaatgt tatattcttt
     2461 tgccaaatga tccgaaaaat cttcaaaaga attcacttcg catttttcca acgttgcttc
     2521 gatcgcattt tgcacaaact cttgataatt aatttttcca tatttcatct tagaaggatc
     2581 gtttgaataa agattggttc tcttttctaa gttttcatag tactcattat tcactgattc
     2641 aagaccatgt tcattgagga cttgatcgtt aatttttcgc attagttcta aatctttagg
     2701 gtcaatacgc aaccttttac atgtttccat gttaatatta ttaatgacaa gatgactatg
     2761 aaaatgatct ttatcattgt gacttgcaac aatgatttgc gaatttggaa aagctttctc
     2821 tgcaaattcg acagctattt cgtgcatttt atcaatactt aactctttag aagtactctt
     2881 atcaaacgaa tgaataacat gaaacgcttg tgctatatct ttagatttat tcatcttggt
     2941 acgtactaaa ttcatttctt gcagtgcttc ctcactatca tcactacaat ttattcctga
     3001 gactgcaaca catttttctt tttttgaagg attagctgca tattgtaata gcgaggataa
     3061 atttttcttg cgtcctttcg atgttatagt agctcccaaa gttgtttcac ctcatctttt
     3121 aaacttgcaa taatctgaat tgagtgaatt aaattccgat caactttttg ggaatactct
     3181 tctttcgtac ccggtctagt ttgcttttct atccaatatt cgaaatattc tttttctact
     3241 tctgataatt taaaaaaaga gggggcactc cgattggact ccaaaaaaat gtcatctgga
     3301 taactaatca tcaacttttt tgctatgcta ttcgcaagtt catctttaaa aatgttgtaa
     3361 cggctagcat caaaactaaa atgtaggctc ttttcttttt tatcttcaga ttcttctcta
     3421 aaatatttag caatgatatt taatgccttt accgcttgat tcaaattgtt accaatttga
     3481 tttaactgtt tggttaaaac taccctatca tcttcagcta acaacggcgg ttcatatttg
     3541 agtcccttta accgctcacg gcaaacgtct gctctagaaa ttcccctttg ttttgcaatc
     3601 aatgtcagtt caagataagt ctttttatcg acacgaaaat ttaaggcctc actatgtttg
     3661 cgattttttt cgtacatttt ttgtttatca tccattgatt atcccctcac atttttttct
     3721 agagagcaac atttgaaatt gctctctatt tcatttttca tctttaattc ttcttgaatc
     3781 atcagttcaa tataattagt gaccgtgatt ttttcatttg ttgcgttctt tactgattca
     3841 cataattcgt caagttctaa gaaatcatgc gtactaatct ttagaattaa ttgcatgaca
     3901 atccccccct tttatttctt aataaattat cgaaaagtgg attaggatca ttaagtgtat
     3961 ctctgaataa ctcattgtct tcataagtga atcttcgtct tgattttgat cccttatcat
     4021 tttcacggat ttcactatca ataatcactg tttcatgatt tgaatcaaat tttttcgctg
     4081 actttctgtc ttcaacgata ttgatttttt gtgttttttc tttcatttct ccactatctt
     4141 tttgaatgtt ctcccgaaca tctactgttt tgaatccttg actacttgta gaactttgta
     4201 cattttctcg gacatctact gtttttggtt gacttgcact gctaccttgc acattctctc
     4261 ggacatccac tgatttaaac ccttgactac ttgtagaatt ttgtacattt tctcgaacat
     4321 ctactgtttt tggttgactt gtactgctac tttgcagatt ttctcgaaca tcaattgtct
     4381 tagtaccttg agcatttgaa gaactttgta aattctctct tacatcaaca ttccgtccaa
     4441 catgaccatt cagtgaacct tgaatatttt ctcggacatc tactgatcgg ccaccatgac
     4501 tacttgtaga attttgtgga ttttctttga ctaatttttc taatcctgta tttgaatttt
     4561 tagcttcttg aattctttct ttggtcaacg cttcttttcc gtcaatatta gcgcctgtcc
     4621 gcccttctac acgtttcctt aacattattt cctcgcgatt cattgcttca ggcgtgtatt
     4681 tcgcttcatg acgcttctga ttaattaatt cttcagcaga cttagcttga ccgtgcatag
     4741 catttttacg ttcttcaata tcttgttgga ctttttgctg aattgcgctt gcaggattat
     4801 tattcgattc agcattattt ttcattgctt cacgcatacg ttcagaacta gacataattc
     4861 tatcggcatt ttcaacttta gcaccatctt ttctatctga aagacgtgtt ggcacggaat
     4921 cagcatattt tccagtttca tcttctttat ttttagctaa cataggtttg ctatttttat
     4981 taatagcgga cactgtacca tcatctaaac cttctttaaa cttattcttg gtatcattag
     5041 ctatatcacg catactctta aatggttttg ttgctctttc actagccatt gtagctccat
     5101 ctgaagctaa accggataat ccacgttcat gggcatatcc caaagctctt ccagttttgc
     5161 cagctttttt tgatagagtt tcgccgaaat tcttttctgg ttttcgatct tctccttctc
     5221 cgcttccttt agcacgatta aatccatttg atccttttct aaacagcatt gcgcccattc
     5281 caaaagcaga agcaagttgc ccatagcctt ctttgagtcc aatatcaaca ccaaagtatc
     5341 tcatgactgt tccactgcct ttgattaaaa caaaaacggc acagatataa gcaattgttt
     5401 ttataaaaat atttgttgag ataccttgtc ctgagttcag aaaattgata aacatagcaa
     5461 acattgataa cccaaatcct tgaaacccaa cagttaagta acattgtaga atatctgata
     5521 gaacaacttt tgaacgctgt cctgtctcaa tatctgttgc aaaaacaaga acacctaaca
     5581 cacgtttaaa tgcaagttct agtattgctg taatgatcac aaacgcgctg aacacatagg
     5641 cgattgctaa cgctgtcaac ccaattgaaa tcccccatag atttgcttgg tatcggtaat
     5701 agcctgattt caagctatca gaaaatgttg ataagaagtt gtcagaaaac tttgtagcaa
     5761 caaattcatt attagaattt tcaactaatt cataacgaag attgtccgca attggatttt
     5821 ctttttccaa tttatcaatc actttatccg ttaacacttg tgtgagatcc gtctgttgaa
     5881 aattttttcg tgttaaatta tttcgatcct ccgttttatc aatgcttgag tactgatctg
     5941 ttttattaat atatgctaaa tctgttactc cttgttttat caatgaccaa gcgactccgt
     6001 catctgaatt tgtaatcgta gtagagtcct tataaaatgt tttagcaaat tgaataccac
     6061 tagaaatcat ggttggcatt aaaagtatta atgctataga catcactacg ttcattccaa
     6121 ccgattttaa atccactgtt ccttttccag taatcatttt gtaaccaaca aacatcaatg
     6181 aaaggatcat taatccaatg acaattgtat ttacggctga ttgataaaca ttatttagcc
     6241 ctgtactttc aatgaaattg aacaaatcca gcacatctgg aatcatattt tgaatcgaat
     6301 ctgtcacagt aaacatgcct tttaccacag cccatccgag ccaaatcccg aactgactaa
     6361 ggaaatctcc attgtacttt tttaggtaag gtctataaag agaattattc aaaaactctg
     6421 tttgactttt tgtgtcatca aaccaattga aaatactcgc atgagcaata ttacttgtaa
     6481 aaataatcat agctatcaga acaagaaaat taacaaccaa ccaatttatt tttttattgg
     6541 caatcttttt ttcaattatt ttcatcgtta cctcccttag cccattttct gaggctctca
     6601 gagagcttct gagccatttt ttgattaatt atgacatgag ttacacaatt atcattttca
     6661 gctcttaaat cgagaactat agtgttgaag caaatagtct cgattaattc gagactatct
     6721 gtattttgat ctagacacca aacttgatct attaaacgat ttttaatcaa aataacccct
     6781 cctatattca aagttctcaa atgaagtgga gatacccctc catttcattt gactcaattc
     6841 tattcttatt gcttatctat tccttcgctg atttgcaatt caattagttc taacgcttta
     6901 ttttttagag aagcaaagat ataatcgtct ttttgtttga tacgatgatc tgtttttgct
     6961 cttcgtatca agtcacgtaa agtcattgat agctgttctc taacatgatc tacaaaatac
     7021 ttgttattac aaatttcata gataattttc agttgaattt tttgttcttt ttcagcagat
     7081 ctaatcgcat atttgaattt tttgtaccag ttgtcgaatt gacgataatc actattgcta
     7141 gccagtttta agcaattcac aatactttct ccccatattt gtttaagaag attttcttca
     7201 ataaatgttt cgatcaattc ttcatctaat ttattatttt tagttgaaag tgattcaaga
     7261 agatcatcat tatgtgattc tttaatatct ttactatctt tataagactt atataattct
     7321 agttcatttt ttgaactatc agcaaaattg tttttgtctc ttgttttcat tgatgtgtcg
     7381 catagttcaa attttgaatt atcaacagaa tattgatttg ttccatagtt caaattttga
     7441 actatcaagt ttttttgtct attttcttga tgggttatct tattaatttt ataaacatca
     7501 tctttgcaca gaacaggctt taacaaataa taaaaatttg ggaacatttg cattttactt
     7561 ttattgaagc cattttttct tttcacaaga agtttaactt tttgaagttc atctagtgct
     7621 gataagattg tattttttga cattcccgaa atttctacga gatttttgat ttcaaatatc
     7681 aaaaaaattt tttgattctc atcatacaag ttatttttca atgaaagtct atttctatct
     7741 tttagtaaca tataaactaa cttagaactt agtttcattt tcttatattt ttcatttacg
     7801 tttaaaaaaa taggatattg atagtataat tctgcatata tatcagaatt agaatagcct
     7861 tgcttttcaa ctaagctttc aacttctttt tcattcattg tcacacctgc ttctgatttt
     7921 atttgctata atagattcaa ataaagtttt ctcagaactt tattattgag tgttcccccg
     7981 atctgcaaac tgacgggaac actcttttta attttttcta cttcatcaaa tttaaaaaac
     8041 gaggtgtttt ttaaatttga tgactcaata tccatttttt gcattcttct tttgaaaaca
     8101 actccatttt ttctaataaa ataactggca atcccattct tttaaatttc aaaaaagttg
     8161 tatatgatac attcaaatat ttagccatct cttttccttt tagaaatggt ctttctaatc
     8221 cggtatcaat tcttgcttga tcaatagcct cttttgtcat ttcaaaaaca taatttttca
     8281 attgattcgt ttgttgttta tctaacatta ctgtaattcc tgcatccatt atttttctcc
     8341 tctcattttc tatttcttca atcagccaac tcgccaagtt gtatttattt agattttcaa
     8401 atcattaagt aaccaattta ccaacttttg ataagtagat gctctaatac tttctttgct
     8461 cccattttcg attttgacat atgttttccg atttatacct atcaattttg ctgcatcgtt
     8521 tttagtaagt ataagatcgg ctctatgtct tcttaagctt ctaatgaatt gatcattaag
     8581 agttatcatt cagcgcctcc tttcaaatta ttcactttgt ggtttttatc ttaataagca
     8641 cattttaaac cacaaaacga gtaatatcaa ttaaaatatt actcaatttg tggttttcat
     8701 gctataatag tttggaattt actatagggg gcgttacttt gaatagaata aaattttata
     8761 ttgattccag taaaaagacc attaaacaaa ttagtacaga aactggtatt aactatacaa
     8821 ctcttacgaa ctattaccgt ggtgagcgaa atcctagaaa tactgaaaca tggaatatcc
     8881 ttgctgatta tttccatgtt cctgtaagtt atttgatggg attagacgaa acaaattcca
     8941 tagaacaacc taatcttgat agaataaaaa ctgctcgttt agaaaaaaag gacaaaatag
     9001 tcaagaatat tgccagtctt gactcagatt attttactga attatctaat ctatactttt
     9061 ccatgacttc tttacttgaa gtcaaacttc aagaagatga ctccgacttt ctttttttaa
     9121 aaagtattct agaggactcc tttgaataca cgattctatt tgccaaacaa aagaagttta
     9181 ttaataaagc tttaaactta aatatcgatg aaagaaaaga gttagaatct gagttatttg
     9241 gcaacttaac ccaagtacag gttaacaatc taaaaagaga gataacagct cgattaatat
     9301 caaaacttca ataaaactaa ttattcaatc agccaactcg ccaagttatc agattggaga
     9361 gattagttat gtcaatacaa aagtacacaa caaaaagcgg tgcacgatat agtgtccgct
     9421 tgtatgttcg taaagatgaa aatggaaagc aagaatacta tgtaaaaaaa gggtttaaaa
     9481 ctgaaaaaga agccaagtta cacgaagcaa gaaaaaaggt cgagattgaa gataccggct
     9541 atcataagcc ttctaatgac ttgtttataa cagtgtatga agaatggttg ccttcttata
     9601 ggaatcgtgt acaagaaacg acatatcaac gtacaaaaga ccttttccgc ttgcatatac
     9661 taccaaaatt cgggaataag tccatttcta agattactcc tgttcactgt cagaatgcag
     9721 tcaatgaatg ggctttaaaa ttcaagaata tcaaacagct taagtcttac agctctcaaa
     9781 tatttgagta tgccattttt gcagagctat tacaccgaaa tcctatggca aatacaaatc
     9841 taccaaagcg ggaaaagaaa gaaaatgaaa attacttttc attagaagaa ctaaaagctt
     9901 tttgggatat tctcaataaa gaagagcctt taaaacatat tttaatcttc cagcttttga
     9961 ttactactgg tatacgtaaa ggagagctag ctgctcttca ttggtctgac atttattttg
    10021 aagaacagct aatgtatgtt agaaagagct atgcaactat tcgaaatgaa gagaagaatg
    10081 caagaagaaa aactgtcagg attcaaaaaa atacaaaaaa tatatcttcc gaacgaatat
    10141 taccaattga tgcacaaact attgaaatgt taaaaatctg gaaaaaagaa caagctcgag
    10201 agcttttgca gtttggcata aatacaaata caaaaaatca acttatcttt acctatgtaa
    10261 atgcagacgg tgacattaat caacctttgc atgcggacta ttcaaataat atattgaaac
    10321 gtttagagaa gaaatataaa tttaagcacg ttactattca tggattccgg cacactcacg
    10381 ctactcttct actagaaggt ggagcaagta tcaaagaaac acaagatcga ttgggtcata
    10441 aaaatgctga aactaccctt aacacttatt ctcatgttac tgaaaaagct caacgaaatg
    10501 cgttagaaaa attcattgct tacactggtt tataatattg tcattacctt tctcattacc
    10561 agaacgaaaa aaagacacct tctcgcaatg agaaagtgtc tgaaatcgtt ggtataacaa
    10621 cgattaacgt tttgaaaact gtgaagcttt acgtgctttc ttaagacctg gttttttacg
    10681 ttcgaccata cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta aacggaagtc
    10741 aggatcaact tctagcaatg cacgagcgat accatgacgg atagctcctg attgtccagc
    10801 gtatcctccg ccgtttacgt taacaaatac gtcatatgcg ccttttgttt cagtaacagc
    10861 gaaaggttga ttgattactt cacgcaagtc agcatgtgga atgtattctt caacatcttt
    10921 tttatttaca gtaattttgc cagttcctgg tactagacgt acgcgagcaa ctgcgttttt
    10981 acgacggcct gtgccgatat attgaacttg tgccaatgaa attccctcct attagattaa
    11041 gtttgtgatg tctaaaactt ctggttgttg tgctgcatgt ggatgttctg ctccaccgta
    11101 tacgttcaat tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca taccttttac
    11161 agaagtttcg atcaaacgac gagaattttt agcacgcaat tcgccagcag tgattgattt
    11221 caatcctcct gggtagttgc tgtgacggta ataaattttg tcagtcgctt ttttacctgt
    11281 taatttcact ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt
    11341 gaaagttggt ttatttttac cacgtagtac agatgcaaca actgttgaaa gacgtcccaa
    11401 tggaacatca gttgcgtcta ctacatacca tttacgttct acttcgcctg gtttggccat
    11461 atatgttgta cgcacgttat tttcctccaa tttaagttct gatgtttgac atacacttcg
    11521 agtttccggg gctctttgtg gggcaaacaa taccatttaa catattaccc gctaatctag
    11581 tcaatgtcaa cgtttttctg cttgattgaa aaatttatat gaaactttac ttcttttctt
    11641 tcttttatat aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa
    11701 agtcttgtca caacccctta ttttgaataa acggtgttct cctgccaact cctcggtcac
    11761 aagtcacaat atccataaaa cataagaagc tgttttttaa aattgtttct tgtgactcgg
    11821 agctgaacgg caggcttaca acctcttggt aaacggtgtt caatcagctg accctcggta
    11881 ttagctcaaa aaacggcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca
    11941 ggtcaaaacg ctgatttcac aacctctttt tcggtcaagt tcaacaacag tttttacgcg
    12001 tcgatctctg gcgctttgcc caaatgagct tggatcatcc agatacgttt ttctgtggct
    12061 gttttgaatt cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg
    12121 attccttttt ggtaaagatc tgctagataa cgatagcctg caacaagtgt ttccaaacgt
    12181 tctggaatag tacgatccca gcggccaatt tcatcaggga tttttgtgtt atctgcaaat
    12241 tcacgcaatg tagaataagg tgaaccgtct aatgtgatca agcgttcgga tacttcatct
    12301 aactgagcgt tcaaatcatc catgaactca tccatcattg ggtgcaatgt aaggaaacct
    12361 ggtcctctca tgtaccaatg tgtttggtga acaacaacag acatttggct tagatctgca
    12421 actaattgat ttaatacttc tttggtttgt tggaatttca tctttcttgc ctcctaacat
    12481 tttcttacac ttaaaataat agtagacttt caatgaaaag tacaacaaaa tcatttacct
    12541 gagaacgatt ttcagttcgc ttttttatcc gtaaagccaa tcatttttca gaatcagcta
    12601 attcttctcg cagttgagca aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa
    12661 caaataatgg atagattttc tgatattttt tatgatcagt ttcttgtact ccaacttgct
    12721 ctttcaattc tatcaattcc tgtgcttcac tgatactttg gatctttccg atgcttctcc
    12781 agcctaaaat cacagctcct aatgcggatg cttctgtgac agtagggaaa acaagcgggt
    12841 aacccagtac atcagccatc atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag
    12901 cacggatctc tgtgagctga ttttcctggc aaactccttt taaaatccga taaaggttga
    12961 gacagatccc ttctagaatc gccttcatca tttctttttc tgtatgttgc cgctgtaaac
    13021 cgataaaact gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg
    13081 gatgaaaaat cagaccattg gcacccgctg gcacaaaagc gatagccgaa agcagtgcat
    13141 catatggatt ttctcctttt tctttagcag attgaacgac ttcctgtaaa aatgtatgcc
    13201 ctgcccaatc aaagatgcca gcaccattgc tagtcgctcc tcccactacc caatgtttct
    13261 gatccagaac ataacaaaac gtctccattt ctggatggaa ctgaggctga gtagtaataa
    13321 aacgaagtgc tccgctcgtt cctacggtca aagcggcgat cgtatcacca aaagcaccta
    13381 accctagatt agaaagcggc ccgtctgccc ctcccaaaac aaatgatata tcatttggca
    13441 aacctagttc tgctgctgtt tttgatggta gacctgttac ttgataggtg gaaggaaaga
    13501 cttgcggcaa ttgctctatt cgaatgttca agtagtcaag cacttcttct gcccattgga
    13561 attcatggat atcaaaatat ccagttcctg aagctgttcc gtagtcgctg atcagttcgc
    13621 caaacaatct ataaaagata tagtctttga taccgacaaa ataagcagtt tttttgaata
    13681 attcaggttg gctgttttgc agccacttga ttttgttcaa tggtgtcatt ggatgaatcg
    13741 gcgtcccagt ctgccgataa aattctttgc caagcacggt atatttcagt ttttctgctt
    13801 cgtccgctgc tcggttatct gcccaagtga tcattcgaga taacggtgtt ttctcttcat
    13861 ccaacaatat caggccatgc atcgcagagg agaaggagac aaattgaatg attgtgtctg
    13921 aaggcaactg tttaattact tcttttataa cggtgcagac accttgaaag atcaattcct
    13981 gatcttgttc tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg
    14041 ctgcttcttg ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta
    14101 atccgattga aacgttcatt ttttcacctc aaattttatg tatgtacaaa aatttagttt
    14161 aagcttgcgt atcttggttt tgaaaagggg gagatggcgt gttactttat tttatcattg
    14221 gttgctgtat cggatcattt ttatgtttaa ccgcccagcg gattccgtta ggtcactcta
    14281 ttatttatcc tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga
    14341 ttccgattct ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc
    14401 ccttctacta tttgttggca gaagccttat gtggtggtct ttttgcctgg ttctttactt
    14461 tttcttccgc aagaaatttt tcgacgttca tatggatgct ttcggctttg ctattttcgt
    14521 tgatggattt attttacttc atggtccatt ctcacacgct attctgttct tgggcaattt
    14581 tatggatttt ttggttgcag gccggcgtct tccaatggca aagtgtgctg ttaacgttta
    14641 tcgtaggcgg tgtgtgcctt cgctatggtc aatcctatct cggagctggc gatactctgt
    14701 tgatgctttc ttggagtggt ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg
    14761 ctagtctgtt tggaatatcc gttttttctg tttactttat ctgtcataaa aaaaagttgg
    14821 aaaagctgcc tttcattcct tttttgagca gtgcgttgct gattgtcctt catcttaggt
    14881 attgaaaaaa ggttgtgaca aacgacttgt cacaacctct gatatatagt gttctcttac
    14941 caaatccttg tgacttggag ctaggcgaca ggttcacaac ctctccacta aatggtgttc
    15001 aatcagctga ccctcagtat tagctcaaaa tgctggtttt acaacctctg atatacggtg
    15061 ttccataagt agctttttcg gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac
    15121 ctgcacgtcc agaaccttct cgataaagat tgtaatggag cttgtttttc ttgtaataat
    15181 cctgatggta atcttctgca ggataaaatt cgcttcttgg ttcgattttt gtcacgattg
    15241 gcttatcgaa tcgcccgctg tcttgcaatg cttgttttga acgctctgca atatttcgtt
    15301 cttcttcatc attatagtag atcactggac gataagaatc accgcgatca gcgaactgtc
    15361 ctaatgcgtc agtcggatcg gtttgctgcc aatagatttc caccaactga tcataagaaa
    15421 taacttctgg atcatattcg atctctactg cttctgtatg ccctgttgtg cctgttgtta
    15481 cttgttcata agttggattt ggtacatgcc cgccagtata accggatgtc acagaaatga
    15541 tccctggctg ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg
    15601 ctttttttgt catataaaat cccccttttt tctcatcata ccttgatttc caaaaggagg
    15661 ataacgtttt gcttgtgaca gaaaaaacgg aaactattat atacgtgata cggcttctga
    15721 taaatgccaa aacgaacgta cttgatcaaa aacggttaaa tcaggaatca catattcagc
    15781 agaaggaaga tttatgtttt cacctattcc caatacatgc atgttaccag ccaaagctgc
    15841 ttgacaacca gcttctgaat cttcaatgac caagcatgca tttggcggta cattcaattg
    15901 ctgtgctcct ttgagaaaaa cttctggatc aggttttgct tttgatactt gcgtgccgtc
    15961 aataaggaca tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt
    16021 gcttgctgat cccaatccga tcttgatgtt tgtttttttc aagtactcaa gaacagctat
    16081 tgcaccaggc aaaacggatg tttcatccat tttttgaatc gcttgaagat atagattgtt
    16141 tttttgctca gcaaaggctg atttctcttt ttctgagaat ttctgttcca tttttccaaa
    16201 tgacagcaat cgctctagtg actcggttct ggatattcct ttcagttgct cgttttcttt
    16261 ctcagtgaat ggaatggaca gctggttagc taaaaccagc cacgcttcgt aatgataacg
    16321 agctgtgtct acaagaacgc catctaaatc gaatagtgct gctttgattt tcatcgatta
    16381 cctccttgaa aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga
    16441 ttatcagtgg cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa
    16501 acggccgaaa tgccaacatc tgctgctttt tcgcttcctt taccaacact tccgcaaata
    16561 gctatcacag gaacgccctc tggtactctt ctagcaatcc caactggcgc ttttcctttt
    16621 agactttgtc catctagacg tccttcgcct acgattacga ggtctgcttg ctgtacccgt
    16681 ttatcgaact ccagtaaatc caatacgtag tcgatgccag agactatttt ccctgaagca
    16741 aatgctaaca gtcctgcacc cattccacca ccagcactac tgcccgatgc ctcgattact
    16801 tgaggggcaa atctcatata aaaatcatgc atggcttggt cagccactgc taattcattt
    16861 tctttcaagc ctttttgtgg tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga
    16921 ttagtaacat cagtaatgac agtgattttg gctttaatca gctcaatagg cacgttcgtt
    16981 ttatcaactg tagcaatttt acctagattt tctcccagtg cttctacttt ttgattatct
    17041 tgatcgtaaa aagcataacc tagaccttct gccatgccga tgccgccatc gttggtacta
    17101 cttccgccaa cgccgatgat gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt
    17161 tctcctactc ctcgcgtgga taacctaagt ggctgttgtt ggtttagtgg gacatctttc
    17221 aatccgacga tttctgccat ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt
    17281 tctttgtttt tgccaaatgc attagtaacg actgctgttt gccgcttcaa ccttaaactg
    17341 tctgctaatg atgaaaccgt tccttctcct ccatcgccta taggtagcag atcatattca
    17401 gcctgaacaa aaacttcttg aaaacctgat tgaatggctt gtgctgcctc taaggctgtc
    17461 agactttctt tgaaagaatc tggcgcaatc agaatacgca ttattttccc ctccgttcca
    17521 agatcagttc tttttccagc tggtattggt ctccattgac aaaaataggt aatgtttctt
    17581 tcgtctcttt ttttattact atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt
    17641 tccaaataaa aggaaaagaa attttttccc atgctgaagg aagtcttgga gaaatagaaa
    17701 gagttccatt cgttactgta atacctgaaa aaccaaatat aactgctagc catagtgctc
    17761 ctaatgaagc agcatgaatt ccgtcatctg atgaatgcgg atctggtcct aaatcaatca
    17821 ggcacgcttc ttggaataaa tgatacgccc attccatttc ttggatacgg caagcctcta
    17881 tggcgtgaat cgctttactc aacgatgagt cgtggattgt tttgttttca tagtaatgca
    17941 aattcttaga aacgattggt tgagaaaaat actcaggtaa taagtataac agcatgacaa
    18001 gatctgcttg cttgctgact tggagttcat ttacttcgct acgggcataa tcaagtaaaa
    18061 tagactggct gccaggacgt tctttgtaag tggtcaaatc aataactggc ttactgagat
    18121 acgtgtcatc ttgcggaatc agctgttctt tgttaggcac tggcaaatat aacttatcta
    18181 aaaaattttg acattctttc aaaaaaagcg gatcgccagc ttgatattta acagctaagg
    18241 ccacattata atgcgccaga tagttcgtat atgcattatt gtcgatatat tctgtgtatt
    18301 catctggacc aatgactctg tgtatctcta aatgatcatc gacttttaca gctctgctca
    18361 gccaaaaagc tgctgtttca tgaatgagcg tttttccctt tgtttctaaa aacgattgat
    18421 cctctgttgc ttggacatac tcttgaacgg cataagcaag gtctgcactg atatgatgtt
    18481 ctgcggcagc tgaagcaact tcttgtcttg ttccggtttt gatattcaat gcagcaaatt
    18541 tgggtgtctc ttcctgacca gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt
    18601 gataatctct tgctttttgc cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat
    18661 tctttgctac ttcaggaaac tgatataaaa agaaaggcag gataaaaatt tccgtatccc
    18721 agaaaacatg ccctttataa ccttcgccag ttagtccctt cgcccctaca ctaacacttg
    18781 gatcattgcg gggagtcata atctgtaaat ggtaagcagc gaagtccatc gctaactgat
    18841 cgaaagaaga atcgccttgg atcgttatcc gatgctgatg ccaaaaatct gtccaataag
    18901 acatcgactg ctctaaaatt tctgtataac taacgctttt ctgggcttgg gcaatacttt
    18961 tcttaaacgg gtctgtttgc tgaagagagg tagagagcac gatgatcttc tccagcgtaa
    19021 aggacgtatt ttttctgatc ttttctgaaa aggaagcggt gatccggcga ttttttgctt
    19081 ggaatacacc tgctttattg aatttagctg caatcccaat atcaatttta ctttctgttg
    19141 tctggtagga tgcactgatc aaatcgtcac caaatactcg cagttctttt tcaaccaact
    19201 gctgtgtacc aaaattggtt tgctgtccat cgatacccgt tttcacatct acttggcagt
    19261 cttcggtcaa cggcttgatg accactttca gggctgcctg atggagatta ttttgatcag
    19321 caacacgaca agtaatgact tccacttttt ttccttgagc agtttcaatg agcgcatagc
    19381 gtttaagctc tcccatttct aaatctagtg cccgctcgta tgcaagaact ttcatcgtct
    19441 caagagaaaa atgttcaccg tcgattttta tttcccatct gatgacatct gggagattca
    19501 ctaactcggc tgattctgct cctaaaggtc ggttatatac accagctgca aacattcccc
    19561 ttacttgatt ccgatagtct tcctcctggc ttcctctgat accaaaaaat ccatttccaa
    19621 tggtcatctg actcgcatac ttattgatat actctaaatc aaactgtttt tcttctactt
    19681 tcataaggta ataacttctc ctgtttctgc cgaacgatat aaattttcca ccagattctg
    19741 gatgacatat ccttgccagc catctgcaat catctgagtc tggtcaccga gcgcccgatt
    19801 cacaaaatga gccatactta gttcatgtgg gtcattaact ggcgtggtct tttgtaagga
    19861 aaccagcttt ccatctacat cttcgaatag ttcgcctgga tacagattag cacctgcctg
    19921 atcgccacaa aaagccacat tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt
    19981 cagttggatg atcttgccgt tttctaattc cagtgtgcca aaaattgcat cttctactgt
    20041 gaatttgctt ggatcccatt tcccgaattt ccctgaattt tttttcgtac caattttttg
    20101 gaagctgtac gcccgaacgc tttttacttt aggaaatcca gtaatataca tcgctgtatc
    20161 aagcatgtga atgccataat cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa
    20221 cgttccccag cctggaacac cagaccgacg aagcgcattc gcttctacgt aataaatatc
    20281 cccgagaaca tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa
    20341 tccataagcc aaaatctgcc cgtttttatc ggctgtctct gccatctttc ttgcttgatc
    20401 agctgtcata gctggtggtt tctcgcacat cactgaagca ttagcttcga gggcagccaa
    20461 tacatgttcg aagtgaaagc gattgggtgt acagacactc acgatatcgg gagaaatctc
    20521 attcagcatt tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc
    20581 ttctgctcgc tggctgactg gatcaaccac agcggccaat tctaagacat ctgatctttt
    20641 ttgataataa gcagcatgta ctttttctgc tacttgtcca gcgcctatga ttgctacttt
    20701 cttcatttat ttctctctcc tttgcttatt tacttattgt tattctttct ctattctttc
    20761 tctaaatcat ttaaatgacg cgctgtttcc agacagtttt tcaagtattg caatgattcc
    20821 tgataaattt tcacttcatt ttctcctcgc aaacgacatt catataccat ataaccttca
    20881 tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt
    20941 tggtagcgat gattatctgc taaatggata tgacccacat actctttttg cgtagtcaat
    21001 gcttttgaaa tatcatcttc ttcaatattc atatgataaa aatcagcgat gatctgcaca
    21061 tgtttcaatt gattagagtc aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg
    21121 tcttggtatc ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc ggctacttta
    21181 tctaaataac gaagtgattc gctgactgct ttatagtctc cttctttgct tcggggtgaa
    21241 accatcggcg gtaatcgata agtgaacatt ccccaagctg ctgggataac gattcctttg
    21301 cctccaacct cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc attaagacgc
    21361 cgttcttcga taaagtcacc gatccaccca tcgtagccgt tacaagctgt cactactggg
    21421 atccctgttt ctttgattgc ctgtttgact gtgtcgatat tttctattaa caacttaccg
    21481 tcgatttcgt attcttcaaa tccacagtct ttgataaatc taaatttttc taaaatgtct
    21541 cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg ttctcctcct cggacttgaa
    21601 cgcccatttt gatactttct tctgggtgct ggtctacata acgcatgtaa gcttctgcgc
    21661 tttctgcaaa tggcacaact ggatcaatga tttcttcaca atccaagtaa ccgttcatca
    21721 ataattccca acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa tctgggtttg
    21781 gttcactaga tgctcgagca aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa
    21841 gattaaaccc agctggaaaa ggtttagcaa aggcaacata agaaatgatc ccgccatagc
    21901 cgatccctat taaagaagct tgtaatgcgg cagcactgcc actcgtttca ataactgaat
    21961 ctactccacg tttatctgtc aattccttta gacgaaaacc gacatctgct ttcataggat
    22021 ctagcgtgta atcggcaccg tgtttttctg ctaattctct acgctgcgct atgggatcga
    22081 tcccaacgat ggtagtagca cctgcttttt tagctaattg aatcgcaatt tgcccgatag
    22141 cgcctaaacc gaagattgct acagcatcac ctgctcgaac atgtgcttcc cggataccac
    22201 ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt ccattgatcc aaccgatcaa
    22261 gtttacgtag tttataatta tcaacaccgt ttgtaatcag cgtttcacga atgggtccat
    22321 aagtgcagac taactctcct ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg
    22381 taccgacaat catatttcct aattgaaatt cgccaaatac cacaccgcgt tggcttcctt
    22441 cttctcttgg caagaatagg cgccattctt ctgaaaattc ttcatcgata aatggactta
    22501 atcctctaaa gtcgacaatt tctgttcctg gttttggagt agcgaaaatt actcggatac
    22561 gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt gaatccggct attcttggtt
    22621 ctaatgcaac taattttttc atcttttctt tcctctttct tttcttttta tccttttacg
    22681 ccgccttcga ctaaatcacc tttaataaat cgttcagata acgcatacat aatgatgact
    22741 ggtaaagcgg tcaccaatga agcggccatc attcgtcccc aaatgtagtc tggtgtactg
    22801 aataatgtgt tcaaaccgat tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa
    22861 gcaaataaat aatcattcca cgaaaccata aagcaataaa cgaagacaga aatgattcca
    22921 gaaatagcca atggtataat gatccgaata atgatctgga tccggctaag accatcgatc
    22981 attgctgctt cttcaatatc agtcggaatc gtgtcaaaat aactttttag catgaatatc
    23041 gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt atcatacaaa
    23101 cctagaccgg aaatgatttt aaataatgga acaacgatca aaataccgga aaacatataa
    23161 acggtgtaaa agctagcatt gattttcgag cgagctttga agcgtagttt tgacaaagcg
    23221 tatgccccgc tgatcccaat cactactgca gttacggagg ctatcaagga gatcactaaa
    23281 ctatttttga aatacatgat gaatggaaaa atctcagcat tgaaaatatc ctgataatgt
    23341 tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc
    23401 ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa
    23461 atcgtcgcgt aaaataagat tttcctgctc tttttatttt tcatttcaag tccaccttct
    23521 ttcgtgagag caagacgacc gatgcaataa tgatgaacaa tagaatcgat atagctgctg
    23581 cttttccaag atcattgaac gcaaaggctg tctgatacaa ataaacgcct aagatattga
    23641 ctttattcgt cagtagatag acatctgtaa acatatagaa catccagata gatcgtaaaa
    23701 caatcacagt tgctagtact ggtttgattg ccggcagtgt cacgatcttg aacttctgcc
    23761 aagcatttgc accgtccatt tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa
    23821 tagccaaaaa tgaaataaac gcatacggat agtatctcca gatagcaaac aaaacgacca
    23881 taaagaagct agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc
    23941 tatctactaa cagatcattg acgattccat aactattatt gaacatatat ttccaagcaa
    24001 aaatcaaaga aatagatggc gtaacataac ttaataaaat cagtgaacgc accgttttcc
    24061 taaatctgaa cttgcggtta agcaaaatag ctacggctaa tcctaaagct gtactgcctg
    24121 ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga acgataaaat tcactatcac
    24181 ttaaaatttc aagataattg cttaatccga taaaaacggc gtctgagtta ggattcagcg
    24241 gcatatcgaa aaaactgatc tgaatattgg aaagcattgg aatcagcact aaaacagcta
    24301 ataaaagcat gcttggtgct aaaagtccca tggctaattt taagtcagaa tgtttccttg
    24361 gacgattcat tgaaacctcc ccctgataat ctgccaatag ctatctactt ttttcgaaca
    24421 ccattggcag aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga
    24481 catcttcgtt tccgacagtt acttggttca ctgcttttcc gatcacacct gagcttgtga
    24541 tatctcccat tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt acttcatcaa
    24601 aagcactagc aatctcagat ggcagctcgc caaaagactt gatgacttca ttttctttgt
    24661 aaccatccag ttcgactact tgtttattca ctggttgagc accaccagga gacatcaaga
    24721 cccacttttc catgtttttc ggttgagata agaaagctgt gaatgcttca gcagcctctt
    24781 tttgtttctc gtctagacca gaagaaatcg tcaatccaga tactgtgcca tagacagctt
    24841 gatctttttg tgttgggata gcaaaaccga tattttctga gttccccgct tcataaacag
    24901 aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt tccattcatg aaagcatctt
    24961 tgatttctgt cacatcatta gagcctggca tcgtgtattg ggataagttt tgataaaacg
    25021 acaatgcttc tctcatcttt tccgtatcga tcgttacctc gccttcatca tccaaaacat
    25081 ttgctccatt tgacaaagca aattgagaga acgcttgctc actcattgtt ccttcagcag
    25141 taggcatagc aattccatat tgtttattct ctttatctgt gaaatgttga gcaattttta
    25201 aaatatcatc ccaattttct ggttctgaaa aaccagcttc tgataatttt tctttgtcat
    25261 accaaatacc ttgaacccag ccgctaattg gtgcagcaat atagcttttt ccatcttctg
    25321 aacgaacaag gtttttggct ccgtcataat aattatcttc tccagcttcc tcgataacgt
    25381 tttggactgc cgtctgatca atcagttcat ctttatccat cactttagca aaatcctgac
    25441 tgacttccat tacttctggt aatttaccgg aacgagctaa cgtaacgatt tttgtattga
    25501 acgctgattc ttctaccggc acttgcttga ttttgatgtc tggattttct ttttcaaaat
    25561 cagcaaccag ttcattgatc acacttaatc gttcctgttc gacagaagag tgcatgaatt
    25621 gaatcgttgt ctgttgtgat tcgttagagc tttgactttc accacaagca gtaagaagaa
    25681 atccagtagc tagcagacag gtagtaacaa taattttttt catttttttc cacctcttat
    25741 tttattattt tcagccacat atattcatac ggctggaata tcaactttcg ttcttgactg
    25801 caatggataa tttgtccaga aaacaggtct aggtaatctc cttttggtaa aagataacct
    25861 gctgattgat tggaaaaatt atggataact aagactttct cttccttcgc ttcgcgaata
    25921 aaactgataa aagaatctgc tgagtccagt aagtaaatca aactattcgg atggaaggct
    25981 ttttcttttt ttcgaataga tagcatctgg gtcaattcac catatactgc tgttcttaat
    26041 ggatctgtct gcaactcttt ctcaatatct gctaacaaaa atttttgccg attgattttt
    26101 cgattctctc ctgttttttc tacgcctttg taatcatttc gacttcccag cacactttga
    26161 atataaattg ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt
    26221 ttgcttctcc attccgtcgt atctgttgga gaacttaacg catctagata cgtagcgttt
    26281 atctcatatg gactggtcgt tccatctggg tttttcttgt agtttactcg ggcaccctct
    26341 ttcttcagct gttccactaa ttcaaggatc gttttttccg gcagaatacc tcgtaccgga
    26401 ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg
    26461 aaatcgatgg tttttaacca ttctttcaac ttacttgtat tacccgaacg aatcgcttga
    26521 agggtcaaag gtggtagcga aaactgatag accatctgtg cttcattctc gccattccca
    26581 aaataactga tattatcttt gtgtggcaca tttgtctcag tgatcaatac cgttcctgga
    26641 gctgcttcat cagcgattgc acggaataat tggatgattt tatgcgtttc tggcaggtgt
    26701 atacaagaag ttcctagttc tttccataaa aatcccacag cgtccaagcg aataaatgaa
    26761 gcgccttttt ctaagtaaaa caaaagaata tcgatcattt tcaataaaac ttctggattt
    26821 ttaaaattca agtctacttg atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg
    26881 tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg gtctagtcac gctgcttgta
    26941 tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa gtttaaatat
    27001 tcctgaaacc aagtactttc ggcagacata tgattgcaga caaagtcgaa cattaaccgt
    27061 gtttcgcttc tcatttcttc tatatcttcc catgtgccca aacggtcgtt gattgcctga
    27121 taatttacaa cggagaaacc atcatcagat gtatatggga aaaatggcaa gaaatgtaca
    27181 actctaaatg tatctgccaa atacttttga taaaatttct taaatgtacg caaagttggt
    27241 tgctgctctt caaaaaattg atccccataa gtaatcaaga aaatatcttc ttcgtcccaa
    27301 tgcagtttgc ctgcctggcc tttcttctgc tgataaacac tgactttttg ttcaatcata
    27361 gaggcggttt tctcagacaa atctgagtcg cgataaatcg cttgtaaaag ttttgtatac
    27421 atcggattcc cctttctttt ttgggagcgt tcccaacgat ttgatcatat tatggaaaac
    27481 gcttactgtc aatagcattt ttttaaataa ttttgatata cacaaaaata caaataagaa
    27541 agcttatgta tcaataatta atagcttctt aaaaaaatat ggtctctcat gatcatgcaa
    27601 gctattccta aattttcaaa atcatagaca aaaaagaatg tctattgtac tatggaatta
    27661 ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga aacctactat ttatgacatt
    27721 gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag tattaaataa ccagccaaat
    27781 atctctgata aagcaaaagc aagtgtactt caagcaatca aagaattgaa ttatgaaccg
    27841 agcaaaattg caagaggact gtcaggaaat ggttttgacg cgattctagc attatctcat
    27901 cgtacttctc atacgaccta tggcaatcct tttttttcag atgttattca atccatttct
    27961 gccgtttctg aacaaaataa cttcgacttg attctacaaa cggcaaaaac accagaagaa
    28021 gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat tattatttta
    28081 agctctacga tggatgaaac acttcttttt gaattggatc aatacaatat tcctattgtg
    28141 gtaatcggaa aagtcggaaa cgactacacg aacatctact cagtggacac ggataatttc
    28201 caggatagtt acgatttggt ggaaacactg atcaagaatg gacaccgtca tatcggctgt
    28261 ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc
    28321 ttatttgatc accacctaga tatccgtaat gaatggatta tagacggcgg ctttcgctta
    28381 gaagatagca tggcagcaat gaatgattta atgaagctac gagaaagacc aactgcgatt
    28441 ttttctaccg atgcattaaa atcattgagt atttacaaag tggcagaaca atctggaaag
    28501 cagatacctg atgatctttc gatcgcagga ttcagtgatc ccttattgtc ctccctattt
    28561 tcgccacagt tgactcggat agacattccg acaaatcagt taggaatcaa agccacagaa
    28621 cttttattcc aactaatcca ccaccaatac cctgaagaaa aaagaatcat tctttcaacc
    28681 gagattactc tcacagattc gatccaacaa caagaaatgt agaacagaat tataaaagaa
    28741 cagaaacact gaggataagt cagttactct gttctttttt caatcactca tccctcgtta
    28801 ccccctcccg gtattttagc gaatttttct tattttccat ctgttttaga tagctgtgga
    28861 taactataat gtttgtgtta aactattttt tccacaggga agaaaaaact atttttaacc
    28921 acgttgtgta taagttttta tagttcgttt ttttaaacac atttaccctt atttcaatcc
    28981 atgttatttg gggatactta tctctaaaac ataaatagaa taacagacat gtctactggt
    29041 tttgttttat tcgaggaatt attcaataat agctagcaaa atattaaaaa taaccgctct
    29101 atcttcccat caatttattt tgcattcaaa aaatgatcta tctttacttt tgttgattga
    29161 cttctcttgg aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga
    29221 tcctttccca cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg
    29281 tgacaataat ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc
    29341 gtcttctgca gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac
    29401 accgacttct tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat
    29461 cttacctata ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc
    29521 agcatccgtc aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt
    29581 ttctaatgat gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc
    29641 tgagactagc gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg
    29701 taagatatct gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat
    29761 tacttcgata tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc
    29821 acgaagcaag tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc
    29881 gattgatcct tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat
    29941 acgtgctgta taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc
    30001 tttggtacgt gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat
    30061 cactgcttgc cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg
    30121 agtgatatcg tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc
    30181 aggttcaccg attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga
    30241 gccagttgca aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt
    30301 gaagacagaa cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc
    30361 ttcggaaatc aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc
    30421 tttacgggtg taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc
    30481 gcggatggct tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac
    30541 atcttgggcc acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc
    30601 tgtatcggtc atccctttac gagccccgtg agttgagata aacatttcca agacggatag
    30661 tccttcacgg aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat
    30721 caaaccacgc attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc
    30781 agacatcatg aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct
    30841 gtcttttgca gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag
    30901 accacgacgg aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc
    30961 ttgtttttca ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga
    31021 atgtttgtac cctaagtctt tcatacggtc aagcatctta gaagtttctg ttactttgaa
    31081 tcgtttgaag acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa
    31141 ttcttgctct ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg
    31201 tgtttggacg gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat
    31261 gatctcgttg aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc
    31321 agtaaatggt ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac
    31381 atatccgttg cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc
    31441 ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgagaagg
    31501 agtaacaact ggtttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat
    31561 acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc
    31621 gaaatcggca ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac
    31681 taaaactggt tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa
    31741 tactgggtgt tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc
    31801 gattttacgt ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat
    31861 cacgaatggt ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt
    31921 caagaaaggt ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa
    31981 gttttgacgg aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg
    32041 gttacctggt ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc
    32101 ttcttgcagc atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa
    32161 acgtttcaaa cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt
    32221 tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat
    32281 aacgtccata accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc
    32341 caaacggcgg attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa
    32401 ttcggctgct tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc
    32461 catagcagct tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt
    32521 caacaattgt tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa
    32581 ataaatgatt tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg
    32641 aatcccttta aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg
    32701 ttcacggcga actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt
    32761 ataacggata cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat
    32821 ccgctcacag aacaaccctt cgcgttcagg ttttaaggta cgatagttaa tcgtctcagg
    32881 tttttttact tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg
    32941 catactttcg aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca
    33001 aaccgcaaga cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt
    33061 atcttctgcg gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc
    33121 agctttcttt tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac
    33181 agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat
    33241 atccaagaca cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga
    33301 ttccggtaca cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac
    33361 acgtccgact acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc
    33421 gtaagcttcc agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt
    33481 acctcccaat ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt
    33541 gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc
    33601 aaatggttca cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc
    33661 ttcacgaact gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac
    33721 gtggatacct aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt
    33781 catacgtgaa ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag
    33841 gaatggcata tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc
    33901 ggccatttta tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat
    33961 gtttacgcct ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac
    34021 gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc
    34081 gccaaagatc gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt
    34141 gactttccct acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat
    34201 ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc
    34261 aggtcctaat tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta
    34321 tacgtcatct tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc
    34381 ccaagtcatg aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga
    34441 tggtccgtct gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg
    34501 ttggttgtag cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa
    34561 tgcaccgttg tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccgtc
    34621 atgtttacat aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc
    34681 aacccatggt gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat
    34741 caaggcacgg ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac
    34801 tacttgttta ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc
    34861 actttgagca cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct
    34921 gtttgcttgg gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc
    34981 agtaacacga cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt
    35041 atttactttc gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt
    35101 ttcgatcgga cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc
    35161 gcggtcacga gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc
    35221 gccaagaggg tttgtttgat ccatgaactg tgacaactga gaagaaccaa agaattcttt
    35281 gatacttgcc acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc
    35341 ttggatcgac attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg
    35401 gttttgtaaa agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc
    35461 gacattgccg ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc
    35521 tggacgaacc gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg
    35581 ttcagggtct cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac
    35641 accgtcttct gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag
    35701 tgtttccatg acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc
    35761 tggatcaact aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt
    35821 tttgtttact ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg
    35881 agcattcagc aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc
    35941 gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt
    36001 ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga
    36061 accaaatcct aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg
    36121 tacataagag atgtcttttg catcagtttc catttctagc caagctccac ggttagggat
    36181 aacagtagaa ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc
    36241 tggtgaacgg actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc
    36301 ttgttctgtc attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt
    36361 ctcgcggttt gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc
    36421 acgagcttct gctactgtat actttggttc tttcaattca taatctacaa attccaaaga
    36481 tagattgcca ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc
    36541 atctaagaac cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac
    36601 ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc
    36661 agccaagctc ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca
    36721 ataatatttc aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa
    36781 aaaaaaccaa aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt
    36841 ccttatatta aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt
    36901 tttgcaactt catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt
    36961 ctgctttttc gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat
    37021 tcttaattga cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa
    37081 cttctatgcg aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta
    37141 gctactttag ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta
    37201 tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt
    37261 ggctgagcag tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt
    37321 ccgacttttc aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa
    37381 ggaggaaaaa gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc
    37441 tttaggaggt tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat
    37501 tggcgtattg caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga
    37561 aggattagca gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc
    37621 ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga
    37681 tttgctactt ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat
    37741 cccgatgact gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga
    37801 aaaacctggc ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg
    37861 cttactgtta aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga
    37921 agcaaactct aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa
    37981 agtcaaagta ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc
    38041 taaagatgta gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt
    38101 tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt
    38161 caaagacggc ggtctagcaa cagtaggaat cgactacgaa agcttgggca aacaaacagg
    38221 gatcatggca gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc
    38281 gaatgatttg gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga
    38341 cagcatcaaa gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt
    38401 ttcttatcat ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc
    38461 actttccgta tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca
    38521 gggatcgcgg cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc
    38581 tttttagggg gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt
    38641 cccgcccttc ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg
    38701 atgggtgcgc cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca
    38761 ttcggcataa caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt
    38821 gtatgtttac ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc
    38881 gataatctgg caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt
    38941 tatatgattt ctaatggatg cattgctctt tctggctcct tacttgcgca aaacaacggc
    39001 tttgctgatt taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc
    39061 gcagaagttt tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt
    39121 gcggtcattt atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat
    39181 tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa
    39241 aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat
    39301 tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag
    39361 gcattaatct ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg
    39421 gtaaatctac tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt
    39481 tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc
    39541 gcgtattcca agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg
    39601 ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc
    39661 gaacattttt caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct
    39721 cagaaatcgg tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat
    39781 tacagcgtcc agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt
    39841 ccaaaacggt gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta
    39901 tggtcactca tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc
    39961 aaggaaggat cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat
    40021 tattagcgtt gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat
    40081 cttcataatc agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct
    40141 ttccttcttt cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc
    40201 tctccttctt cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca
    40261 aggtacttca attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc
    40321 aactggaagt ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct
    40381 atcgttttga gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa
    40441 aagaaatcca gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga
    40501 cttctgaatc tacgatgaaa gatgccaaac tggcagcagc tcaaggagat tctgtttcga
    40561 agttatttgt tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg
    40621 ctgaacctgg acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg
    40681 aattgcctca atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa
    40741 ccggtcgttc tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct
    40801 gtgggatttt atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca
    40861 ttgggatcgg gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa
    40921 aagccatctc tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca
    40981 aaatctggca agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata
    41041 aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg
    41101 tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga
    41161 agatgagttt gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag
    41221 atttcacaaa aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag
    41281 actctcctta ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa
    41341 tatctataaa gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat
    41401 cagtttacac gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc
    41461 atgggtaacg ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta
    41521 gctagtgcct taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg
    41581 catagcagca tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca
    41641 gttctcgtca attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc
    41701 aaaatcagca gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg
    41761 ctttgcttgt tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa
    41821 ccggctttcc cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg
    41881 gaaatctaat tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc
    41941 tctatcttcc actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc
    42001 aaaccgatcg tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt
    42061 tcctcctttt tatttatcct acttactttt tcctaattct aggataccta cttatatgtt
    42121 actacacacc cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga
    42181 aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt
    42241 aatattcttt aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga
    42301 gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt
    42361 actagtccta gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga
    42421 aacatacatt cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt
    42481 gttaggaatt gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca
    42541 tacagacgga aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct
    42601 tcatgaaggt ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa
    42661 ttcttcaggc atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt
    42721 ggatgtagca cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac
    42781 tccagatgat ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt
    42841 tgctaaattg aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac
    42901 tgctgcagat tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac
    42961 cgggttttta ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat
    43021 cattaatcca aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa
    43081 cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga
    43141 tgcgaatatc tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt
    43201 tcaaggaaca atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac
    43261 gattatagga acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat
    43321 atttatcagc gacaatggta cagatacgac agacacaagc gacagtacag atacatctac
    43381 cacaagtgat agctcagatt catca
//
LOCUS       DAFCNS010000014        43256 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFCNS010000014 DAFCNS010000000
VERSION     DAFCNS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.014"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..209)
                     /locus_tag="LNE23_000750"
     CDS             complement(<1..209)
                     /locus_tag="LNE23_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930626.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISS"
     gene            complement(197..1549)
                     /locus_tag="LNE23_000751"
                     /pseudo
     CDS             complement(197..1549)
                     /locus_tag="LNE23_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(1546..2478)
                     /locus_tag="LNE23_000752"
     CDS             complement(1546..2478)
                     /locus_tag="LNE23_000752"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBK6930627.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2475..3251)
                     /locus_tag="LNE23_000753"
     CDS             complement(2475..3251)
                     /locus_tag="LNE23_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBK6930628.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3305..6490)
                     /gene="carB"
                     /locus_tag="LNE23_000754"
     CDS             complement(3305..6490)
                     /gene="carB"
                     /locus_tag="LNE23_000754"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBK6930629.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6495..7571)
                     /gene="carA"
                     /locus_tag="LNE23_000755"
     CDS             complement(6495..7571)
                     /gene="carA"
                     /locus_tag="LNE23_000755"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBK6930630.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7576..8856)
                     /locus_tag="LNE23_000756"
     CDS             complement(7576..8856)
                     /locus_tag="LNE23_000756"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBK6930631.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8875..9801)
                     /locus_tag="LNE23_000757"
     CDS             complement(8875..9801)
                     /locus_tag="LNE23_000757"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBK6930632.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9814..11097)
                     /locus_tag="LNE23_000758"
     CDS             complement(9814..11097)
                     /locus_tag="LNE23_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBK6930633.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11111..11650)
                     /gene="pyrR"
                     /locus_tag="LNE23_000759"
     CDS             complement(11111..11650)
                     /gene="pyrR"
                     /locus_tag="LNE23_000759"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBK6930634.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11885..12796)
                     /locus_tag="LNE23_000760"
     CDS             complement(11885..12796)
                     /locus_tag="LNE23_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK6930635.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12793..13257)
                     /locus_tag="LNE23_000761"
     CDS             complement(12793..13257)
                     /locus_tag="LNE23_000761"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBK6930636.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13262..13960)
                     /locus_tag="LNE23_000762"
     CDS             complement(13262..13960)
                     /locus_tag="LNE23_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6930637.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13947..14333)
                     /locus_tag="LNE23_000763"
     CDS             complement(13947..14333)
                     /locus_tag="LNE23_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930638.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14480..16150)
                     /locus_tag="LNE23_000764"
     CDS             complement(14480..16150)
                     /locus_tag="LNE23_000764"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBK6930639.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16333..16566
                     /locus_tag="LNE23_000765"
     CDS             16333..16566
                     /locus_tag="LNE23_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBK6930640.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16665..17096)
                     /locus_tag="LNE23_000766"
     CDS             complement(16665..17096)
                     /locus_tag="LNE23_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBK6930641.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17175..17588
                     /locus_tag="LNE23_000767"
     CDS             17175..17588
                     /locus_tag="LNE23_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBK6930642.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17626..18774)
                     /locus_tag="LNE23_000768"
     CDS             complement(17626..18774)
                     /locus_tag="LNE23_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK6930643.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18896..19492)
                     /locus_tag="LNE23_000769"
     CDS             complement(18896..19492)
                     /locus_tag="LNE23_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6930644.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19692..22334)
                     /gene="alaS"
                     /locus_tag="LNE23_000770"
     CDS             complement(19692..22334)
                     /gene="alaS"
                     /locus_tag="LNE23_000770"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBK6930645.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22722..23153
                     /locus_tag="LNE23_000771"
     CDS             22722..23153
                     /locus_tag="LNE23_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6930646.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23198..24547)
                     /locus_tag="LNE23_000772"
     CDS             complement(23198..24547)
                     /locus_tag="LNE23_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK6930647.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24617..25780)
                     /locus_tag="LNE23_000773"
     CDS             complement(24617..25780)
                     /locus_tag="LNE23_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK6930648.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25909..26232
                     /locus_tag="LNE23_000774"
     CDS             25909..26232
                     /locus_tag="LNE23_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930649.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26415..27764)
                     /locus_tag="LNE23_000775"
     CDS             complement(26415..27764)
                     /locus_tag="LNE23_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6930650.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27833..28786)
                     /locus_tag="LNE23_000776"
     CDS             complement(27833..28786)
                     /locus_tag="LNE23_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBK6930651.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28888..30078)
                     /locus_tag="LNE23_000777"
     CDS             complement(28888..30078)
                     /locus_tag="LNE23_000777"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBK6930652.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(30723..31406)
                     /locus_tag="LNE23_000778"
     CDS             complement(30723..31406)
                     /locus_tag="LNE23_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6930653.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            complement(31487..32983)
                     /locus_tag="LNE23_000779"
     CDS             complement(31487..32983)
                     /locus_tag="LNE23_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBK6930654.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            complement(33029..34195)
                     /locus_tag="LNE23_000780"
     CDS             complement(33029..34195)
                     /locus_tag="LNE23_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBK6930655.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            complement(34404..34771)
                     /gene="rnpB"
                     /locus_tag="LNE23_000781"
     ncRNA           complement(34404..34771)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LNE23_000781"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(34793..35212)
                     /gene="gpsB"
                     /locus_tag="LNE23_000782"
     CDS             complement(34793..35212)
                     /gene="gpsB"
                     /locus_tag="LNE23_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBK6930656.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            complement(35302..35840)
                     /locus_tag="LNE23_000783"
                     /pseudo
     CDS             complement(35302..35840)
                     /locus_tag="LNE23_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
     gene            35904..36551
                     /gene="recU"
                     /locus_tag="LNE23_000784"
     CDS             35904..36551
                     /gene="recU"
                     /locus_tag="LNE23_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBK6930657.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            36541..38922
                     /locus_tag="LNE23_000785"
     CDS             36541..38922
                     /locus_tag="LNE23_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK6930658.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            39219..39752
                     /locus_tag="LNE23_000786"
     CDS             39219..39752
                     /locus_tag="LNE23_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930659.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(39878..40555)
                     /gene="nth"
                     /locus_tag="LNE23_000787"
     CDS             complement(39878..40555)
                     /gene="nth"
                     /locus_tag="LNE23_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBK6930660.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(40562..41260)
                     /locus_tag="LNE23_000788"
     CDS             complement(40562..41260)
                     /locus_tag="LNE23_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK6930661.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            41528..42712
                     /locus_tag="LNE23_000789"
     CDS             41528..42712
                     /locus_tag="LNE23_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBK6930662.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(42718..43161)
                     /locus_tag="LNE23_000790"
     CDS             complement(42718..43161)
                     /locus_tag="LNE23_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6930663.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT        12182 a         9322 c         7584 g        14168 t
ORIGIN      
        1 gaagagatta ttctcttctt tctgctaacc agtcttagaa aaatgaattt taaaaaaatc
       61 aaaaactgat cacgatagaa gtttcttttc atacctgatg aaaagatgct tctcgcaatc
      121 agtttttgga tttttgctat tccaattgat gattctggag acacaaatcg gatttctcta
      181 tcttcatcaa gcgcgcttaa atctgccaat tttcaggatc ttttttccat tcttttagca
      241 gagttaattc ttgctcatcg atataacgat cttctaaagc tgcttcaatc aatacagaat
      301 aattggtcaa cgtcataaga gggatttcgg ctttttcgaa atttgctttt ccttttggca
      361 actcatacgt aaagatagcc gcaacaccaa ggatatccgc accttctctt tttgctgctt
      421 cagctgcttc caagacactt ccgcctgtag aaatcaaatc ctcgatcaca accatcttct
      481 gaccttcaaa tatacgtcct tcgatttggt tacctttccc atgatctttc gccttacttc
      541 gaatatagac cattggcaaa tccagtattt ccgctaccca tgcggcatga gggattcctg
      601 ctgttgcagt tccagcaatc acttgtactt cagggtaagc ttctttgatt ttgcttgcta
      661 atccttttgc gatctctttt cggacttttg gatagctcat cgtgattcgg ttatcacagt
      721 aaattgggct tttgattcca cttgcccatg taaaaggatt acttggattt aaaaatactg
      781 cttcaatttc taataagtct tttgcgatac tttgttcaac attcatttta ggttagctcc
      841 attctgtttg aatcgtttga tatgctttat aaggattagt tgactgagtg attggtcgac
      901 ctataacgat aaaagttgag ccgatctttt ttgcctgaga aggtgtgact actcttgtct
      961 gatctcctgc gactgttcct tctggtctta tacccggagt taagcaaaca aattcgtctc
     1021 ctgccatttc tttgatcgct tctacttccc aagcagaaga aaccacacca tctaatcctg
     1081 cttttttggc acagctagca taatggatca cacttttttc taaagaaact tcgattagtt
     1141 gatcggcatg catttcttct tcacttgtag aagtaagctg agtgatagct agaagtagcg
     1201 gacgttttcc accttctgga gtcccctctt ctaatcctcg cattgccgcc tccatcatac
     1261 gaataccgcc agcggcatga acacaggtga gatcaacacc aagctttgcc aatccccgca
     1321 ttgctttttc gaccgtattc ggaatatcgt gaagtttcag atctaaaaat acattatgac
     1381 cagcctcttt tagataacgt acgatttcag gtccttcttg gtagaacaat tccattccga
     1441 ttttcacaaa caatttttct tctttcggga aaaatcgaag aaagtcttct acctcttgcc
     1501 tagttgaaaa atctaatgca ataattggtc gttggtctag ttgattcatc gttcacgttc
     1561 ctctctcact tctttgatta atctttccag cgattcgatg cctagttcgc tcattcgcac
     1621 agggagctct ttaattagtt taggacagat atacggatcg gtgaaattcg cggtccctat
     1681 agctacagca cttgctccag ccataaacat ctcaagaaca tcatccactg tctgcacgcc
     1741 gcccatccca atgattggaa ggctggatat acttgctact tggtgaatca aacgaatagc
     1801 gacaggcttg attgctggtc cggagagtcc acctgtttga ttggctaaaa tcggttttct
     1861 tgtcttaagg tcaatccgca taccaagaag agtattgatc atcgtaaacc catctgcccc
     1921 tccagcttcg attgcctgag cgattggaac gatgtctgtt acatttgggg acaatttgac
     1981 atagacagga acagaagaaa ctttctttac tgcttgtgtt aattgaaaag caacatcaga
     2041 atctgttcca aaagcaattc cgccatgttt gacatttggg caagaaatgt tcagctcgat
     2101 tgctttcaca tttggtgctt cgccaatttt gctacaaact tctacatagt cttcttcaca
     2161 agcaccagca acatttgcga tgatgggtaa ctcattgtac tcctctaatt ctggaagaag
     2221 tttatgcata acaacgtcta gaccaggatt ttgcaaacca attgcattca acatgccaga
     2281 tggtgtttcc gccactcttg gcgtgggatt gccaaagcgg gcatttggag tcgttgcctt
     2341 gaccatgatc gatccaagct gattcaaatc atagtatttt ccgtattctt tgccaaaacc
     2401 gaaacagccg cttgccggca tgataggatt ttttaattct aggccaggaa gtttgaccgc
     2461 taaaggattc tcattcatat aattacctct cctattttaa aaatcggtcc ttcgtcacaa
     2521 actttcacac tttttgttcc tgtttcatct tccggcgtat gacaaacgca agcatagcaa
     2581 gcccctatcc cacatgccat gcgggattct aatgataact ggacattttg atgcgaagaa
     2641 aacagctgtt ccactgtctt caataacccg tttgaaccac atgcaaaaac agctgtaggt
     2701 tccgtcttcg ttcctaacaa taaattaccg acatttccgt gcataccgta tgttccatcg
     2761 tcagtagcga ttcttgtttc tcctagacga ttaaattctt tttcataata aacgacttca
     2821 tgtgtggcaa aacctaaaaa atgcgttggc ttaattcctt tttcgactaa ctgttttgat
     2881 aactcatata gaggagggac accgattcct ccaccgatta taaatacttc gtctcctgta
     2941 tgaagattgg acaaatcaaa accattgcct aaaggaccca tgacatctaa atggtctccg
     3001 gcagaaagag tagagaagac tctagtcccc tcaccttcca cccgataaat gatacggcaa
     3061 gtatttgttt cgtggtcgat atgattcaag ctaatcggtc ggcgtaatag caagtctgca
     3121 cgcggcacga gaatatgcaa gaactgtcct ggttgctgca tttcttttac tagatcacct
     3181 ttcaatgtca tttcaaaaat ctttggtgcc agctgttttt gctgaacgat ttccatcatt
     3241 tcttgtttca tctgtcccct cctgcaacgg tttttcgtct ttttgtctta acatgtgctc
     3301 gccgttaaat agggttagtt gaaaaagcac gggattccat cactcgaacg atcgcatctg
     3361 ctgtgtctaa agaagtaaac aatggaatac catgttcaac tgcttctctt cggatgatga
     3421 aaccgtcttt agaagcattt ccccgatctt tatcaatcgt attgacaacc acttgtgttt
     3481 taccatctct tattaagtcg actacatttt tttctgctgc ttcctcagaa attttcgcta
     3541 ctgtttcaac gaccagacca tttttttcga aataagcagc tgtatgtttc gttcctatca
     3601 gactatagcc aatttctgta aaacgtttag ctaattctag tgcttcttct tttgtttcgt
     3661 ccgcaatcgt aaataatacg gctccatatt caggcaagtg taatccagaa gcttcgaatg
     3721 ccttgtacaa agctttgtct aattgtctgt cagatcccat tacttctcca gtcgatttca
     3781 tttcaggccc taagtaagta tcaactttct gcaatttcgt aaaagaaaag acaggtgctt
     3841 taacatgaac ttcttttgtt tctggataaa gtccgttttc ataaccaagt gtctttagtt
     3901 tctctcccaa aatagctttc gtcgcaattt gtgccattgg aattcctgtc accttactta
     3961 agaatggaac agtccgactt gctcgaggat tgacttcgat cacataaacc tcattttcat
     4021 ggatgacaaa ctggatattc atcatcccga cacaattcaa acctaatgct aagcggatcg
     4081 tgtaatcttc gatcgttttc ttgatcgatt cagataatgt ttgaggtgga tacacagcca
     4141 ttgaatcccc tgaatggaca ccagcacgct caatatgctc catgatccct gggatgagta
     4201 ctgtttcacc gtcacaaatc gcatccactt cgcattcacc accaagcaag taacggtcca
     4261 ctagtactgg atgttctgga gatgctttca cagcatgttc catgtaatct tctaaatctc
     4321 gctggttttc aacgatttcc atcgcacgtc cgcctaatac atagcttgga cgaacaagaa
     4381 ctggataacc aattctcgaa gcgataacca cggcttcttc tttacttgta gcagtatccc
     4441 ctaaaggttg aggaatatcc aactctttca gcgcttgctc gaataaatca cgattttccg
     4501 cacgatccaa gtcttcgatc gttgtcccta agatttttac accagcagca gtcaaaggtt
     4561 cggctaagtt gattgctgtc tgaccaccaa attgaacgat tactcctatc ggctgttcta
     4621 gctcgatcac attcatcaca tcttcgaagg tcaatggctc aaaatataat ttatccgaaa
     4681 tagaaaagtc agtcgaaact gtttcaggat tgctgttcat gatgattgct tcgtatcctg
     4741 cctgctggat tgctttgaca gaatgcacag tcgcatagtc aaattcaacc ccttgtccga
     4801 tacggatagg accagatccc agtactagaa ttgagggctt ttctgatcgt gaactttcat
     4861 tttcatattc ataggaacta taaaaataag gtgtttttga ttcgaattcc gctgcacaag
     4921 tgtcaaccat cttatataca ggaatcaaat cattttcttt tctcatctgg cgaacggctt
     4981 cttcgtttat ttcccacagc tccgccactt ttttgtctgt aaaaccgttt tgtttcgctg
     5041 ttttcaatat cttgtcatcc aacggctgct gtttcaactg ctgttcgatt tctacgatat
     5101 gcaacagttt atctaaaaag aataaatcga tttttgtcaa atccgctaat tcttctatgg
     5161 tgtaccctct tctaatagct tcagctacat aaaacaggcg atcatcttgt gctcgaacca
     5221 ctttttggat taggtcatcg tccgtgatgt ctgccaactc aggaagttcg atatggtgtg
     5281 taccgatttc taaagaacgc acagctttca ataaagactc ttcgatattc cgaccgatag
     5341 ccatcacttc accagttgct ttcatctgcg tgcctaaaag ccgttctccg ttttcaaact
     5401 tatcaaacgg ccagcgagga attttagcta caacataatc taatgcaggt tcaaattctg
     5461 caaaagtcgt tcctgtaaca ggatttttca tttcgtctaa tgttaatcca agcgctattt
     5521 tagcagctag cttagcgatc ggatagccag tcgccttact tgctaacgca gatgaacgag
     5581 aaacacgcgg attgacttca atcacataat aattaaaact atgtggatca agagcgagtt
     5641 ggacattaca gcctccctcg atcttaagtg ctcgaataat cgaaagagaa gcatcacgaa
     5701 gcatttgata ttcatggtca gacaatgttt gactaggggc aaatacgata gaatcacctg
     5761 tatggatacc caccgggtca aaattttcca tattgcagac aacgattgca ttatcagccg
     5821 aatctctcat cacttcatat tcaatttctt taaaacctgc aatacttcgt tcaatcaaac
     5881 attgggtagc gggggaaaga cttaatccat tttctgatac gatacgtaat tcttcttcat
     5941 tttcacacat acctccacca gttccgccta aagtaaaggc tggacgaacg atgacaggat
     6001 agccgatttt atttgcaaaa tctacagctt cttctactgt attgacgatt tcagactcgg
     6061 ggattggctg attcaattct ttcatcaact gtttgaatag atcacgatct tctgcttgat
     6121 cgatcgcaga aagctttgtt cctagaagtt ctatgttcaa ttcttctaaa atacctgatg
     6181 ctgccagctc cattgccata ttgaggcctg tctgtccgcc taaagttgga aggagagcat
     6241 ctggacgctc ttttcgaaga atccttgata caaattcaag tgtgatcggt tcgatatata
     6301 cttgatctgc cacttcttta tccgtcatga tcgtcgcagg attagaattt actaaaacca
     6361 cttcatatcc ttcttctttt aaggcaaggc aggcttgtgt tcctgcgtag tcaaattctg
     6421 cggcttgacc aatgatgatc ggaccagatc caatcaccat aattttcttg atatctgtac
     6481 gtttcggcat ctgtttactg ctcctttcct gcgtccatca tttccataaa ttcatcaaat
     6541 aaatgtagtg catcatgcgg accaggggca gcatctggat gaaactgcac actgaaagcg
     6601 ggataatctc tgtgtttgac tccttctact gttccgtcat ttacttctac atgagttacg
     6661 atgagttttt ctggatcaat cgtggcttca tcgactgcat agccatgatt ttgagaagta
     6721 aaatcaattc ttccggtcgc tacttctcgt accgggtgat ttaatcctcg atgtccgaat
     6781 ttcattttat aggtatctgc accgttggct aaagcaaata actggtgacc gagacaaata
     6841 ccaaaaatcg gtacttttcc ttggattttt ttgatcattt cgattgcttc tggcacatct
     6901 tttggatcac caggtccatt cgtcagcatg actccgtctg gtgatagcgc taaaatcgtc
     6961 tcggcatctg tgttataggg aagtactgtg aggttacagt ttcttttact caattctcgt
     7021 aaaatactat gtttcaagcc gaagtcaatg acaatgactt tccgacctgt tcctggactt
     7081 ggataaggtt tgttagttga gacttgctgc acttgattgg ttgctagaac ggttgctttc
     7141 agctgatcaa atgcatgttc gaaggaatct ccagcatcaa ctatactagc tttcatcgtt
     7201 ccgacttcac ggattttgcg agtaagggcg cgcgtatcga tacctgatat tccaggaatt
     7261 cctttttgtt tcaaaaatga atctagcgtc atttggtttc gccagtttga agctacacgt
     7321 gcatgttctt ttactactac tcctttgcat gttggagaaa tcgattcata atcatctcta
     7381 tttaccccat aattacccac cataggatag gtaaatgtaa tgatttgacc gttaaaactt
     7441 tgatcagtga tagtttcttg ataaccagtc atacttgttg taaaaacgat ttctccgaag
     7501 acactagcat ctgcaccaaa agcctttcct tcgaaaactg ttccatcttc taatataagc
     7561 aaacgttcca tacttttatt ctcccttctg ccacacaagt tttccgttga caaaagtcat
     7621 ttgtgtttcg ccttttactt tccagccgat aaaaggtgta ttctcccctt ttgataaaaa
     7681 gtcttcttta tcgatcgtgc aagtttgtgt aatatcaaaa actgctacat cagcgggggc
     7741 accaaccgtt agcgttcctg cattcaaacc aaaaatctca gccggtttaa ctgccaacca
     7801 attgatcact tgttccaacg tgaatcgacc agtttctaca aaatgagtat agatcagttg
     7861 aaacgctgtt tcgcttccta cgataccaaa tggtgatttc atgaaacttt ggcttttttc
     7921 ttctaatcca tggggggcat ggtctgtagc gatgcaatcg atcgtaccgt caagcaagcc
     7981 ttcgatcaaa gcttctctgt cctcccgtcc tcttagcggc gggttcattt tccaaaagcc
     8041 gaaatcttcg ggaatatctt cgtcgatcaa aataagatga tggggtgaaa cttctgctgt
     8101 tacgtggatt cctgcttttt ttgcgtctct gattacacgg acactttctt tagtagaaac
     8161 atggcaaaca tggtaatgaa caccagttgc ttcagctagc aacagatctc tagcaatttg
     8221 agaagactcg gttacgctta agatcccggg aagaccaagt tcttctgcct tcttgccagc
     8281 gtgcataacg ccgccaaata ataaagattc atcttctgta tgggctacta gggctttatt
     8341 gttttttgca gcttctttca tagccagata catcgtccca gctgtttgga cacctacacc
     8401 gtcatttgtg aaagcaaaag caccttcttc aatcagtgct tcttgatcaa ctaatttctc
     8461 acttcgcaga ttttctgtga ttggcgcata ctgcaatacc ttcacaactg catcttttct
     8521 aatgatgtca tatacttgtc tcagcttttc tgccgtatca ggtacaggat ttaaattcgg
     8581 catcgcgcat acagtagtga aaccgccacg agcagctgag cgagtcccag cttcgatcgt
     8641 ctccttgtat gtaaatcctg gctctctcaa atgaacatgg acgtccacta aaccaggtgt
     8701 gatcaattgt ccctttgcat caaacacttc atcaaattcg gcttctgaga atcctgtacc
     8761 gattgctttg attcgcccat cttctatcca aatttctgct ggtgtcgtca tattttttcg
     8821 tgtattgatc tgaccgtttt taatgagtgt tttcattttt tcatcctcct ataattacgc
     8881 ttttccattt agcactgctt ctaagattgc cattctgacg aatactccat tgctcatttg
     8941 ctgaacgatc cgcgactgta atccttccac taatgaatca gctaattcta catcacggtt
     9001 aactggtgct gggtgcataa tgattgcatg tttttgcaac ttcttcgcac gctcaactgt
     9061 taacccatgt tcctgatggt agccttcttt tgagaaactt tcttttccat catggcgttc
     9121 gtgttgcacc cgaagcatca tcatgacatc aacttgatct agtagttcat ccaatggcat
     9181 ataatgtccg tacacttcga attcatcgtc gtaccattct ctaggacctg agaagaatac
     9241 ttgtgcgcct agacgtttta acatctgcat attggattta gctacgcgag agtgagtgat
     9301 atcacctacg atggcaactt tcagaccttc aaaatgacca aattcttcat aaatggtcat
     9361 caaatcaagc aagcattggg ttggatgctg tccgcttccg tctcctccat tgataatcga
     9421 acactggatt gttttacttt gtatcagttc gtcataatag ttctcatcac catgacggat
     9481 aaccgctacg tctacgccta atgctgacat cgtcagtact gtatcgtata gtgtttcccc
     9541 tttttggaca gagcttgtgc ttgcttcaaa ttcaatcact tctagcccta gttttttctc
     9601 cgctacatcg aagcttttat gtgtgcgagt actattttcg aaaaagaggt ttgtagcaaa
     9661 gtactggcgc ttttgcggag tccaattagc accttgcttg aattcctgcc ctctgcggat
     9721 caagcccata acttcttgat cagtaagtgc ctcaacggtc aataaatgct ttaagctgat
     9781 acgttctgat ttgatgatca taatgtttcc tccttagttt tcttcgcttc gtgaagtttc
     9841 tggtaagata agatttaaaa tgatgccaag tacagttgct aatgccattg ccgacaatgt
     9901 aaatgtgcct acttcaaaga cgagtccgcc aattcctact actaagataa ctgaagcaat
     9961 caacagattt ttctttttat caaaatcgat tttgttctcg attaaaatct tcattccact
    10021 tgcggcaatt actccaaata agataaaact gattcctgag atgactggtc cagggatact
    10081 taaaatcaaa gcgcttaatt ttcctacgaa tcctaaaatg actgcaaata ctgcagctcc
    10141 accgatcaca aacacgctat ggacacgtgt gatagccaga acaccaatgt tttcaccgta
    10201 acttgttaca ggaggacctc cgacaaaggc tgccacgatt tgtgctaatc catcacctgt
    10261 caacgtctta tgaagacccg gttcttcaaa gaaattacgt ttagttagtt tattcaatac
    10321 cataagatgg ccgatatgct ctgtcatcgt cacaaaagcg atgggtgcca ttgttgtgat
    10381 tgctccaaga tatagctttg gttcatattg tacaaaggga atctcaaagt taggaacagc
    10441 aaaccaggca gcgtctacga ttggcttagt atccacgatt ccaaacagca aggctacgat
    10501 gtatccgcta ataataccta gtaaaatcgg aatcaatccg agaaaacctc ttagccacat
    10561 gttgaagaaa attgttaatc ctaatgtgat cagcgctaca gcaatatact tgaaatcata
    10621 cgtgccctga ttatacatag catttgttgc tgcatttgcc gcaagtccca acccgataac
    10681 catgatgact ggaccgacga cgattggagg caagatttta tccagccatg ctgaaccgat
    10741 ctttttgatg atcagcgcaa caatcaaata gactaatcca gttgtgactg ctccttgtgc
    10801 aatcgctgga taaccatctg ttttcatcaa aagctgcatc gctgcaatga aagcgaagct
    10861 gcttcctaag tacgcaggta tctttccttt agtagtaaat aagtacacca atgtacctag
    10921 tcctgaactg actagcgcaa tacctggatc gatacctact aagatgggga ctaacacggt
    10981 tgctccgaac atcgtgaaca agtgttgtaa acttaatcct acccaatgtc ctggggttgg
    11041 gcgatcgtgg atatctaaca ccacgtcatc attatgaaat ccttttttct ccgacatgtt
    11101 tctctctcct ctaatcttcc tctttcaaaa tcatgatgcg atcttgtcca tcaagctctt
    11161 gcatctctac taaaatctct tccgcccgag atgttggaat gttcttccca acataatctg
    11221 cacggattgg caattctcga tgcccacgat cgaccaggac tgctaatgaa atttttcttg
    11281 gtctgccaaa atccattact gcgtccattg ctgctcgaat cgttcgtcct gtatacaaaa
    11341 catcatcaat caaaataact tcttttcctt ctaaggaaac tgggatgtca gaagaatgaa
    11401 gttccggttc ttctggattt tcttttttgt catctcggta taaagtgata tccagttctc
    11461 ctacaggaat atcgatatct tctagctgct tcaggcgttc ggcaattctt gatgcaatgt
    11521 agattcctcg tgttttgata cctaccaata cgatatcttg gatactatgg tttctctcga
    11581 taatctcata agtaattcga gtcaatgctc gcttcatggt tacctgatct actacttctt
    11641 ttgcttgcat tttcattccc cctacttttt tgcataaaaa aactcctgcc ctaagaggac
    11701 aggaggaatg tttagactga gcgtatactg atacactgat agctatacta tgtcaatgag
    11761 caatctactt atggcgcacc atacgtatcc ttgctcccac ccttcttagc ctcacgggac
    11821 taaacttaaa ggaattctat taagttttat gctttcgttg ttaattacta taccgttatc
    11881 taaattaaaa tgcaatgttt tttcttaatt tttctaaagt tttttcaaaa acttctggaa
    11941 gaggtgcttc aaagatcatc ttttcgtttg tagtcgggtg cgtaaaacct aacatttgcg
    12001 catggagaaa ctgaccattg cctttcaaag ttttcttcgg tccgtatact ggatctccag
    12061 ctaacggata accgatatac ttcatatgca cacggatctg gtgtgttctt ccggtttcta
    12121 gttttaattc aaccagagta aatccttcga aacgttccaa tacagtaaaa tgtgtgacag
    12181 ccgacttccc atcttctctt atcgcctgca ttttccggtt ttccttcgaa cggccgattg
    12241 gcgcattgat ggttcctttt tcgtgaggaa tctctccatg gactaaagca acatatttgc
    12301 gcaaagaggt cttatctttt aattgctgag ctaatgattc gtgtgcctgg tcatttttgg
    12361 caaccattag cagtccggaa gtatctttgt cgattcggtg aacaatacct ggacgaataa
    12421 cgccgttgat cgtagaaaga tccttgatat ggtacatcag cgcattgacc attgtgccat
    12481 gcgtatgacc tgctgaagga tgaacgacca ttccttgagg tttgtttatc accgctacat
    12541 cttcatcttc gtaaacgatc gtcaacggga tattttctgg gaccacatct aatgtttctg
    12601 gttcggggat caagacagta atctcgtctc cttgttttac tttgtaattt gctttgactg
    12661 gctgtccatt gacagaaacc gctccatttt tcagccacag ctgaatttgc gaacgactgt
    12721 aatcggtgaa taaattagtc aatactttat cgatcctacc agattcatgg tgaatcgtcg
    12781 ttttaagttc tgtcattttt ttctttgatc cttttcatct aaaataatat aaattaacat
    12841 acaaatcact ccacatacaa gtgtcacatc tgctacgtta aaaatcggaa agctcataaa
    12901 atctgtctgg aacatatcta ctacatagcc aagacgaata cggtcgataa aattgccaat
    12961 cgcaccagct agaatcaaac tcaatccaat catgaaccag cgattacctt tttcacgatt
    13021 tttaatcatc aatgtgagaa tcacaacgac tgcaatgagt gtcactatag taaagaacca
    13081 catcttccct tctagcatac tccatgcggc tccagtgttt cgaatatgat tcagtgacaa
    13141 aatacctggt ataagatttt tcgtctctcc aagcgctaga ttatcagtga tccaccattt
    13201 gaccaactga tccactccta ctagcaaagc gctgaatatc caataaataa ttaacaaaaa
    13261 atcaatcctt ttctatttgt ttataaatat cacgaggtcg tacgatcccg agcagattac
    13321 catcgggatt cccatcttta gtgataagta cggcttccaa agtcccttgc tcacgaaaca
    13381 tcgcttcaac ctcgtaaata tgtgttttag ctggaacaaa atgataattt gtgtactttc
    13441 cgttttggat gatcaaatcc aacgctcgtt tatttgttaa atcaaccgga cctttttggc
    13501 tttcttttgc tagccaaaac ccgatattac gtaaagtaat caatgctaca aattttcctt
    13561 tattgtataa cggaaattgt gaataccttt tttctgctac tatctttaac agttcaagta
    13621 acggaagatc ttgttcaaaa cctgttacac gttttgcaaa tcgaggcagc acggtttcag
    13681 gctgaatcag ttcttgttcg atcagctgga ttcgcttgac tgtccaatca tttggctctg
    13741 caatgacaaa atcgactgcg atccgatcat ggacgatcgc attccttaac tgagcaagct
    13801 gtaaaagatc atcttcgtat tttctgatca ttaattgttt tctttgagct aggcgtctga
    13861 caagttcggt aaatcccata tttcgtgcat ttcccatctc gtctctcatc catttttcga
    13921 ttcgattgaa actgattaaa aaaagatcag agtttgccat aattttcctc cttgatcaaa
    13981 tatgcccggt tttttatata gaaaaagaac gcaatcaaca caataactgc aaaaaaactg
    14041 aaaaataaat tttgtaggaa aacggccccc aacccagttg ctagaaataa aaacggatag
    14101 atttgaagaa tcaagtaatt cattgtgatc acataagcag gctttgcttc tttcactgta
    14161 taaacggggc tttttagcca ataaaaggtg acctgcatcg taaagggcac ttccagatca
    14221 agcgttatct tttgttcatg actgagaaca ctgcgttttt cattgttgat tcttagctga
    14281 atcgagctac ccataccgta aaaaccggtt tttcttataa atgtcgtttt catcataccc
    14341 tcctattatg ctttagtata gctcaaaata caataaagaa aaagaatgat cctagtgtaa
    14401 acaaaattaa ttccctatcg tctaaaaaaa gaagctgtag tcaaaataca gctccttcga
    14461 tggtagatta taatcaagct taaaacaatc ccaacacttc tccgtcattt gtcacgtcca
    14521 tattcaatgc agcaggtttt ttaggcaaac caggcatcgt cataacatct ccagtcaagg
    14581 ctactaaaaa tcctgcccct aattttggga tgatttcgcg gatcgtgatg gtgaaatctt
    14641 ccggtgcacc tagcagtgaa ggatcatcag aaaatgaata ctgtgttttt gccatacaaa
    14701 tcggcagttt gtcccatcca ttcttcgtaa actcttctat ttgtttttgc gcttttttgc
    14761 tgaaaacaac accatttcct ccgtaaattt tcttgacgat tgtttctgtc ttttcttgta
    14821 tcgtttcttc taggcggtat aatggcttgt aattagcttc ttttctatca atcaatcgta
    14881 ctacagcttt tgctaaatca acgccgcctt ctgctccatc tgcccataca ctcgctctct
    14941 tggcaagaat cccctgattt tcacacaagt gctccaacag tgtgagttca ttgtctgtat
    15001 ctgttacgaa ttcattgatt gcgacgatca caggtagctg atattgttcc atattacgta
    15061 tatggcgctt taaatttgca aaacctactt tcaaagcatc aagattctca tttttaagtt
    15121 catctttttt cattccgcca tgcattttca atgcacggac agtggcgaca atcaccacag
    15181 catccggcgc ttttttcaag ttaggtactt tgatatctaa aaatttctct ccgccaagat
    15241 ctgcaccaaa tcctgcctct gtgacaacgt aatctgctaa tctcaatgct gtcttggtag
    15301 ctaaaatact attgcagcca tgagcgatat tggcaaacgg tcccccgtgg acaaaagctg
    15361 gtgttccgta aattgtttgt acaagattcg gcttgattgc atctttcagc agtaatgcca
    15421 gagcgccttc tgctttcaag tctccagcag tcacaggctg acggtcaaat gtatagccga
    15481 caacgatcct tgcaagacga gcctttaaat cttcaaggtc tgccgcaaga cacaagatag
    15541 ccatgatttc gctggcaact gtgatatcaa acccatcttc tcttggaaca ccttggattg
    15601 gcccacccaa gccaacaatt actttacgca gttcacgatc attgagatcg actacccgtt
    15661 tccaaatcac tcggcgggca tcgatgttca attcatttcc ttgatgaata tgattatcta
    15721 aaagagcaga taatgcatta ttagctgttg tgatagcatg catgtcacca gtaaaatgca
    15781 agttgatatc ttccataggc aatacctgtg catatcctcc gcctgcagca ccacctttga
    15841 ttcccatcac aggaccaagt gagggttctc gcaatgcaat aacagatttt ttattgattt
    15901 tattgagcgc gtctcctaac cctacagtga tcgttgactt tccttcgcca gctggtgttg
    15961 gattgatgga agtaactaag atcaaatgcc cctcagcgtt atcctttgat cgattgattc
    16021 cagaaaaatt gattttcgcc ttatatttcc catacagttc caaatcgtct tccttaagat
    16081 ctaatttact agccacttca acaatcggtt ctagctctgt ttcttgtgaa atttgcagat
    16141 cattcttcat ttgattaccc cttttcacga actttattca acaaaatatc ttctttgtta
    16201 agatatgcca attatacctt tttttattca gaaaaaaaac ataaaattac aggtttttaa
    16261 tttatttgac gtacttataa atactcttta taatgaaggc tatgatcaga ctttagaaag
    16321 gtcgtgagtg ctttgaccac aggaattgta aaatggtttg acaacaaaaa aggatacggc
    16381 tttatcagtt atgatgacac agaagaaatt tttgtacatt ttactgcgat tgaagaagag
    16441 ggattcaaaa ctttggaaga aaatcaagtg gtcgaatttg agatcatcga agggaatcga
    16501 ggaacacaag ctgcccatgt caagaaaggc aacgttcaga ctaccgatac tagtatgcat
    16561 gaatagataa acaaatagag tgagacgcta aacgggatga accgtacgtc tcactctttt
    16621 cttttcctgt aaaaattcgt ttttttaggt tctgattttt aaaattagct ttttcttttt
    16681 ttcgtataag gataaggaat tgattctgat ttgggtccgt ttttttcaaa atgatcaaaa
    16741 aggactactt gatctttttt tcgcatcatg aaaacagatg gggctttctc atcttgcaac
    16801 atcatttgca ccccagtatt agttggcgtc aaagatcgaa tctgtccgac agatatttct
    16861 cggtctttcc aaaaagcagc ataacgtata atcacagagt ttgattttaa aataaaagaa
    16921 cgccgcaaac ctagaaatag aaaaacaaga aaaataccta aaacaaaatt gcttttccaa
    16981 taaggacctg tgttttctaa agaaagaatc agactaaaaa acagaatgat caaggtcaat
    17041 gaccaataga tgatcgtatg tgccagttca ggctgccatc gaatattttt tttcattctc
    17101 taacctctca ccgattcagt aatgatataa tcttaccata acattagttt gaacaaaaag
    17161 gagaatctct ttttatgtta aaagtataca ttgatgcctc tacaaaaggc aaccctggtc
    17221 ctagtggagg tggaatcttg attgtctatc aaggtaaaca agaacagcta tctatcccgc
    17281 tttctatagg gacaaaccat caagcagagt tcgaagtctt tttaaaaact ttagaatttc
    17341 taaagaaaaa cgacttacag aatgaaacga ttttctgttt ttcagacagc aaaacactag
    17401 tttctacggt cgacaaaaac gcaacgaaga acgagctctt ccttccctac cttcaaagaa
    17461 tccaagaact tctatccgaa ttttctttat tgatcctgca atggttgccc gaaaacaaaa
    17521 ataaaggagc agataatctt gcacgtcagg ctctccataa acaattaaaa gaaaaaaaga
    17581 ctctttaaat taaaaacttt aaggagtctt tttatagttt ctcatttaat ataaaagata
    17641 attgtgatca aacgtaattt ccaattggta atctccgatt ttttcttcta tttgactacg
    17701 taagattctt tctagttctg tatcaacaaa ctggaatttc tcagggacca ctacatcaaa
    17761 ttgcagcgtt tttccttgat gaattactct aaaatcatga atattaagcc ctgtacctgt
    17821 ttcaagaaga atttgcttta actgccgcaa gatattatag tatttttcgt tatcaacagg
    17881 cattggatcc aagtgacaga caagttccac atccaaagac tttttaaagt ctttttctat
    17941 cagatcaata atcttatgag ccacattcaa attcattgat tcatctactt cgatgtggac
    18001 tgatgcaaag cgtttattgg gtccatagtt atgcaccaat aaatcatgat agccaagtat
    18061 atcatcgcaa gcatttaaat gctcttccat ttcttcaatc tctgctggcg ttgggcgact
    18121 tcctaatagt tcgtatacaa aatttcgaag catcgtcaat ccactataaa ggatatataa
    18181 tgctaaagca aaacctatat atccatcaat acgccatcca gttacccatt cgattgctgc
    18241 tgaaagcaat acagccagtg tagtaaagac atcattataa ctatctgttg ctgtagctct
    18301 caatgtttca gaatcaatgg attgcgcaat aacctgatac attttgcctt gtgcaaattt
    18361 caaaaagatg gaaatgatta ggacaaaaaa gacgatgggt gttaaagaga cattttctgg
    18421 atggatgatt ttttcagcag aagactctaa aaattgaaat cctacataag taatgatgat
    18481 cgaaacaaat aacccactga tatattcgaa gcgttcgtgt ccataaggat gttcctgatc
    18541 tgctggcttt gcagcaattt taaatccgat caaagtcaat atggatgaag cagtatctga
    18601 tagactgttc actgcatcag ccataatgga gacacttcct gaaaaaagcc cgatcattag
    18661 cttagagaca aacaagatca gattcgtaac caatcctaag atacccgcta gtactccttt
    18721 ttttgtccgc tgttgcggta tttgtttctt tttttcttct tgtttcttag ccaagttttt
    18781 taccccttct agtttgcttg ttgctcaggt aaaaagatgg agaggacgat tgttttcctc
    18841 tcctctcttc ctttttctat ctatcgtgcg tcctattact attaaaagat ggtcattatt
    18901 ctggtttcaa cggtgttgct gcaaataacg cttttgctag taatggtgtt atgattcccg
    18961 cacctattgc ttctggtatc ccattgattg ctgcaacccc tgctaaggtt ttaaataaaa
    19021 gagcagagtc tacaccatag gcatctgccg ttgcacctgt ataaaataat cccatgaatc
    19081 ctaacactaa gactgtattg accaatgaac ccagtacccc tgcaaaggct gagctgatcg
    19141 ttattgattt atttttttga taaatccagt ggaaaaccaa gcctgctact aagccaacaa
    19201 gtactctggg aactaccgag ataatcggat tcgtgaacac taatgtatca aatggcgttg
    19261 ttggcatggc ataggcacga acaattgtag cgattcccca aaacaagcca atcaccatgc
    19321 catcttttgg gcctaatact actgcagcaa taattactgt gatatggata attgttaaac
    19381 taatgaatcc taatggaata aaccccagcc aaggaaccat cgcttgtacg atgatgattg
    19441 ctaacaagat cgcacgtaag actagtcgaa aggtcttatt tctactattc attttttcac
    19501 ccctttttat ttatactaat cgtaaaaatt tctgtcactt tcgtcaacta aagaccatcc
    19561 gaatccgacc atttgattag atgcgccatc caattgaaaa aagtatgagg gcaagacttg
    19621 tttgtcttcg cccccataat catgtttcgt tcaatgtttg aacttttgtt tttctgtcaa
    19681 acgtaaacgc cctattgttg cgcaacccat tctttcgcag ctttaagtgc atctggaata
    19741 ccagcaggat ttttcccgcc agcttgtgcc atgtctggac gtccaccacc gccaccgcct
    19801 actttcggtg cgattgcttt aatcaagtcg ccagctttta gtccagcttc atttgattct
    19861 ttcgtcattg ccgctagcag actgactttt ccttcttgtt cgtttgctaa aacaagaata
    19921 tctgaaatat ttttttgttt ccattggtca gctaattgac gcagctgatt catatctttc
    19981 acttttactt gtgctgcaat gattgttttt ccattgatcg tttctggatc tttgaataca
    20041 tcaccagctt gttgattcgc taacttgcta gctaattgtt cattttcctt ttgtagctct
    20101 cttacttgct gttgcaattg ttccgctttt gaaacaactt ctttcaattg aggtgattta
    20161 acaattgttg ctacttgttt tagttgtttt tcttcttctt caagtagttc gtacgcttct
    20221 ttacttgtta cagcctcgat tcgtcgcacg cctgcaccaa ttcctgattc agaaacgatt
    20281 ttgaagattc cgatgttttc tgtatttgct acatgtgtgc ctccgcacag ttcgattgac
    20341 cagccgccaa tatttactac tcgaacgtca tgtccgtatt tttcaccaaa caaagccatt
    20401 gcacccatgt ttttggctgt atcgatatct gtttcaattg tttcaactgg gatagcttcc
    20461 cagatttttt cgttgacgat tctttccatt tcagctaatt catctgaagt gatttgacca
    20521 aagtgggtaa agtcgaatcg taaatgtcca ggagcgacta aagaaccagc ttgatttgca
    20581 tgctcgccaa gtacttcttt caatgccttg tgcagtagat gggttgctgt atggtttttg
    20641 atgatccgat tgcgcatcga ttgatcgatc aatagatgat aggtttgtcc ttcactcatt
    20701 ggtgctttta cttctacggt gtgcataaat tgaccattag gcgctttttg aacatctgta
    20761 acaactgcta cgatttcgcc tacttcattc atgatccatc cttgatccgc tacttgccca
    20821 cccatttctg catagaaagg tgtttgatca aagatcattt gagcttcacc ttctgcgact
    20881 tcattcaaaa gagactcacc tttaacgatg accaataatt gactatctga ttcttgttct
    20941 gtgtatccaa tgaatttact ttctactttg atatctgtca ataaagcaga ttgtaccccc
    21001 attgattttt cagtactgcg ggcagagcga gcacgttcgc gttgtgcatc catttctttt
    21061 tcaaaaccag catgatcaac tttcagtcct tcatcttctg ccacctcttc cgttaattct
    21121 accgggaagc catatgtgtc gtatagtttg aagatgtctt ttcctgctag agtcgtcccg
    21181 ttttcgtttt ttacttcttg gatcacttga tttaagattt ccaaaccttc attgattgtt
    21241 tcattgaaac gttcttcttc tgtacggata actttttcaa taaattcttt ttgttgaagt
    21301 acttcaggat aataacttac catgatttcc ccaacaacag ggactaaacg atataagaat
    21361 gcttgttcaa tacctaattt tttcccatgc atgacagcac gacgaagcaa acggcgtaag
    21421 acataccctc tcccttcatt cgatggcaat gcaccgtcgc caatagcaaa tgaaagggcg
    21481 cgaatatgat ctgcgattac tttgtaagaa atatctgtta ctgggttttc accatatttt
    21541 acttgtttgc ttagcttttc tacggcgtgg ataataggca taaacaagtc tgtctcaaag
    21601 ttcgttgacg catcttgaac gattgagacc atgcgttcca atcccatccc cgtatcaatg
    21661 tttttatgag gaagtggttc aaatgtgtta tcaggcttat ggttgaattc tgaaaatact
    21721 aagttccaga tttctaaata acgttcattt tctcctcctg gatagttttc aggatcgtct
    21781 tctgctaaat cgttataagc ttctccacgg tcatagaaaa tttccgtatc cggaccacat
    21841 gggcctgcac cgatatccca gaagttgtct tctacatcaa taatgtgatc tagtggcagg
    21901 ccaacttctt catgccagat gcgttttgct tctgtatctt ttggataaac tgttacataa
    21961 agtttttctg gatcgaatgc catccatttt ggatcagtca aaaattccca tgcccagtgg
    22021 atggcttcat ttttgaaata gtcaccaatc gaaaaattac ccagcatctc aaacatcgta
    22081 tggtgccgtg ctgttttacc gacattttcg atatcatttg ttcgaataga tttttgggca
    22141 tttgtgattc ttggattttc tggcacaact gaaccatcaa aatatttttt caatgttgca
    22201 acaccagagt tgatccacaa taaggttgga tcgttcacgg ggatcaaaga cgcactaggt
    22261 tcaatcgtat gtcctttaga ttggaagaaa tcaagaaaca tttgtcgaac ttgtgcacta
    22321 cttaattgtt tcatgtcatt acctgctttc tataaattaa atacaaaaaa acaccatcac
    22381 agccaaggac gaaaactcgc ggtaccacct tgattgcaat gatgtgtaat cattacctct
    22441 taaactcgat aacgctgagc tgcgtcggta tttctaccaa aatagaaaaa gggagcaatt
    22501 tgattagtga ctattctttt ttcagccaag aaagaatttt ctgtgagtct taaaaaacaa
    22561 atcatatcct tttcgtactt aattagtata cgcattctat tgatcactgt caatatctca
    22621 atttttattt tcatttcctt ttggtcatac tccacgattt ttggtacgat aagaataaag
    22681 gaatcaatac atctctgcta ttcatgacaa ggaggaatat gatgaaaaaa atagttggag
    22741 cggatgaatg gatacgcgca gctagctgct ttttgagata tcaagttttt gtattagaac
    22801 aagggattcc tgcagaacta gaattcgacg gaaaagatat cccgcaacaa acctattatc
    22861 tatttcttga tcaaaaaaga ccagtagcta ctttgcgtta tgaaaagaaa acacctactt
    22921 gcttcaagcc ggaacgattt tgcgtagcaa aagaataccg aggaaaaggg ttaggcggtc
    22981 agttgcttga agctgctgaa aaacgggcgg tttcagaagg ttgtcagact agttatgtag
    23041 tagctgaaat gacggctgta gatttttatg aaaaacaagg gtatcatact tgcacagaac
    23101 cttttattga agatgaagta ctttgtatcg gaatgaaaaa aagtctagct taaaaacaag
    23161 agattgtgac acgccttttg tcacaacctc ttgtttttta tttatgtgaa tttttcttat
    23221 ttttacgagc agtacggctt tgctgtctgc gttcgatttt gcgttctttt ttattttttt
    23281 ctgcaattgc ccaatcgatt tttttcttat aaccaggctt gatctttttc tttttcttct
    23341 tcacaagacc aatcagcgtg ggatctaata catcttttga tttttctcgt ttggcacgtc
    23401 tattacgatc gtatgttttg acgatttccc cgtttttgat ttcttttggt tcaaatgaga
    23461 cgcccaattt ttctatcgca tcaatggctt gctcatcatt tggcgaatat aacgtaatgg
    23521 ccgttccttt taagccatta cgtccagtac gtccgacacg atggataaag aagtctagat
    23581 cttctggaat ttctgcatta ataacatgag agacaccttc gatatcgatc cctctagcag
    23641 ctaaatcagt cgctacaaca tattgatact ccagattctg tacttgtcgc atcactcgtt
    23701 ttctctctct tggggtgatg tctccatgaa tctttgctac tttcaagcct tggtttttca
    23761 agtaatcggc aatttcatcc acatgctgtt ttgtattggc aaatacgatt gctaaataag
    23821 gatgaccaat tgtcagcagt tgataaatca tttgattctc atctttacct tttgttgaaa
    23881 tcagccaatt ttcaatgtct tcagaaataa ctgcttttgg tttgatttct tcaatgattg
    23941 gattttccat gtattttcgt aagaatggac gaagtttttc tggaatagtg gcagaaaaaa
    24001 ctaaaaactg cagcttttct ggtaatcgac ttgcaatttg gtctacttca tttaaaaaac
    24061 ccatatccaa tgtcatatcc gcttcatcga caacaaatgc aaatgcggtg tgaatcttca
    24121 gcgcttgttc attcatcatg tctaatattc taccaggcgt gcctactaca acctgtggct
    24181 gttggtgatg cagacgattt aattgacgct gtttgtctgt accgccgaca aaattcgaga
    24241 cacggatctc cggtgtagaa aatttagcga tttgtgtagc tgcttcatag atctgtgcag
    24301 ctagttccct acttggagct gtaataacaa cttgtacttc ctctttttct gcattgattt
    24361 tttcaaacaa aggcaacaaa aacgtatgtg tttttcctgt accagtttga gattgcccaa
    24421 tgatattttt tccttttgca attaaaggaa tcaaacgttc ctggacctct gtgggctgtt
    24481 tgaagccctt tgcggttaac gcttcattga taaacggttt gaagcggaat atatcaaaat
    24541 tattcattcg tgcacctctt ctctttaaat tatcacgact ttggcattat aacacagaca
    24601 aagcgttttt gctcagctaa tttcgagaat catcatcctt tgtttggcca atcgtgctaa
    24661 cagtatatgt gtgactagct ttttcataaa acagaatccg tttttttctc aaaaatgata
    24721 gcaagatatt taaaataaaa ataccttgta gtaacaagac accgagagaa atgaatactg
    24781 cactcatctg caacaccgta ttcgttgtcg aattcatgat ttgctgaagc aactgcccca
    24841 accagcttaa accataaaaa agaaagtaca gatacccgta gcggatcatc aatgctttta
    24901 aagaaattcg ttcgcggtct tcttccacca aacggatacg cacaattttt ttgccaagtg
    24961 tccatccatt ggttataaac ggtacaatca taaagtatac ccctacttct aataaaagcc
    25021 accagatatg agaagtaact gcccctgaat cgaacgcaat tttaaagaaa atcgccgcta
    25081 ccaaattaat aagagataaa aatgtccagt cgatcaaata agcgaccaag cgtcgagtca
    25141 atgagacagt ctttcctttt tcataagaaa gttcatccat tcgtcttctt gtaggaagca
    25201 taaatgtaaa taacggcgta acgacaaaac caatgattcc acccaatgta tttgtcacta
    25261 aatcattcac atcaaataag cggtatggtc ttgggtagat aaaataaagt ccacttagct
    25321 gcgtcaattc aaaaaataga gaaaggcaga aactagcaat catcgttttc ataaaagaaa
    25381 gccggaaata atatcttaaa taaatgccaa atggtatgag taataaaaca ttaaaagctg
    25441 gttccaaaaa tacactctgc ttcattgccg gcaggtaggt actaggttca gacaagacca
    25501 ataccgtgtt gtctctgaaa tgttgccatg aagcaaataa atgcagctcc atctttggcc
    25561 ctgtcatttg ggcaaccgtc tcctttgccg gaagtggtaa gatcaccaaa aaatacgcac
    25621 acaataaata aaaaacgaaa gaatacaaaa taactgccct tgttactaga aatgttccat
    25681 actttcgata ttcataaata aaaaaaacag atgagatcgc taaggctaaa aatggaaaaa
    25741 caatcagcgc aaaacgaatc ggttcgatat aacttgccat atgactcctc cttgattctt
    25801 tgtatctctc ttatcttagc agtcctttgc tgtttcaagc aagtaacgag gctattattt
    25861 ctcatttctt tcaaagtaaa gtaagataaa cacaaagggg gtgtcgagat gctgaatatg
    25921 tactttgttt ttggcgtacc tattttttta ctgttccttt atgctacgat cgcttatgta
    25981 agaaaaagaa ctacaattca ttatttagga tttattctcc tgattatttc gggtttcatg
    26041 ctagtcttca atcttcagac ttggcagcaa gcacttcttg aaatggataa aatgactcca
    26101 catgctttgt ctaaagtgct tggctaccct gtctatctga tttggcttcc cattttcatt
    26161 tctggttgct tggttttatt aaatatttat cgcggcgtac gccgaatagt tcagttacgc
    26221 aaatcaaagt aaaagacaga aatcggttga gcccgatttc tgtcttttac tttgctattt
    26281 catcgtagac aaaacgacat ttacgtgatc aaaattaacg agtacttttg acatttcttc
    26341 cacgtactcc tcatttaaat aaaatatttt gtttggcaga gaagtgcagt tctaataaaa
    26401 ttcgaatgtt ttagctatct acgttttaaa tagagataaa tcgggatacc agcaagtgtc
    26461 aatactatcc ccatcgaagc taaaaaggtc tgagtgataa gcgtgcttga tacgataaaa
    26521 gtccctccaa tgattgctac tagtggaata aacggataca ttggaacttt gtatggtcga
    26581 aacaaatcag gttctttttt acgaagaaga atgacaccaa caaagaccat cgtataaaaa
    26641 atccaaatga caaagatcag catatccgtt agtgtatcaa aaccaccgat catcatcatg
    26701 ccaatagcga tgactagctc taagatacct gcggctacag gaactttagt tttatcatga
    26761 agtttagcaa agagcttgct aaaaggcagg ttgttttctt ttgcaataac ataagggaga
    26821 cgcattccag taagggtata gccattgatg gttccataaa cagaaatcaa gataccaact
    26881 gtgaccagtc ttccaccgaa gccgccaaaa atcatttttg caacgtcact tgccgcattg
    26941 ctgtttcctg cgactccgtc gatagatgcg gttcttaaga acaccgcatt tactaataaa
    27001 taaacgatca tgattccgat gattcctaaa gaaattgctt ttggcaaatc tttcgccggc
    27061 tttttcaatt ctcccgaaat atttcctacg tgtatccaac cgtcgtatgc gaacatcgtt
    27121 gctagcaagc ccgctcccaa ggctgagaaa aatgaaagat tttctcctgc ttggatcgga
    27181 aacagttgga agtctactcc ttcttgtcgg aacaaaccga aaatgacgat cacaaacaaa
    27241 ggaataagct tgcagacaag cgtaatcgac tgaaaagccc ctccagcttt agaacctaaa
    27301 aaattgatca acaggatcga tacagcagct gtaacagcta cgggaacaat cattgactgg
    27361 gaaagaccaa atagattgac gaactgtgta ccgaaaatga tcgacaaggc tgctacattt
    27421 gctggaaaat aaatcacgac ttgcgcccag cccaataaaa aagctgctgt atttccgtaa
    27481 atccgttcga tatatttaat catccctcct gtttctggaa tagcagctgc cagttcagct
    27541 cctgttagtc ctgcacagac agaaatcata ccaccgagaa accaagaaaa catatgcaaa
    27601 ctggcggatc ctgtaacttc tgctacgctt gcggctttaa aaaatacccc tgcaccgatc
    27661 actgtgccca taacagtcga taaagcagta aaaaatccca tcgtacgttt taattcattt
    27721 tctattttta cttcttttac tggcgtggct tgatattctt ccatcccttg tcccctttca
    27781 taccatagcc aatcccaaaa gtataaaaaa ctttttggat gtggttcttg ttttataccg
    27841 tctctaccaa tttttctttc agtttttcca acatatcctt tgtcatcaca tctaagtcgt
    27901 atttcggcga aaagccccat tcctcttgcg cacaagaaac atctagtgaa tttggccaag
    27961 attctgctat tgactgacgt acaggatcca catcataatc catttcaaaa tctggtatga
    28021 ttttttgaat agatgctttg atctcttccg ggtcaaaaga catcgccgat atattgaagg
    28081 catttcgatg aaccaatcga tcaggatctg cattcatgag ttttataatg gcatcgatcg
    28141 catctggcat atacatcata tccattgatg taccttgatc aatgaaacta gtgtactttc
    28201 cttctttgat tgctgaataa taaatatcga ctgcgtagtc tgtcgtaccg cctcctggca
    28261 aggttgcata agaaatcaat ccagggaaac ggacaccacg agtatctacg ccatatttca
    28321 cataataata atcacatagt agttctccag ctaccttcgt taccccatac atggttgtag
    28381 gacgctgaag agtatcttgt ggggtatgat cttttggagt atttggacca aacgcgccga
    28441 ttgaacttgg cgtaaaaaat ttcagctgga attctctagc gacttccaat gcattgacca
    28501 gcccagtcac gttcaaattc caggctaatt gtggttttgc ttctgcaaca gctgacaaaa
    28561 gagcagccaa atgaatcaat gtgtccactt gatattgttc tgctatgctg cgcatttttt
    28621 cgtaatccat tacatctagg atctcaaaga tatcgttttt ctcaatgact tcaggcatgc
    28681 gtatatctgt cgcaatgaca ttttcactgc ctagttcttg acgtagccgt gtgatcaact
    28741 ctgaaccaat ttgtcctaaa catcctgtca ccaatacttt tttcatgatg atcctccctg
    28801 atctttttta ctactatctt cagatgatta acaatatatg cgtgaacatg ttctctaatt
    28861 ttaaatagac gaaaaaacta aatttagtta aataatttcc atttcttttc ctattttttg
    28921 ataaactgct actgcttcat caagcatttc cctcgtatgt tccgcagtcg gcatatttct
    28981 gatcctgcct gtcccaagag gcacagttgg ataaacgatt ggtttagcat aaatcccatg
    29041 ttcgatcagc ttcttagaga aagtctgagt cactttttcg tctcccaaaa tgactggagt
    29101 aataggtgtt tcagacatac cgatgttata gcctactttt ttcaattctt ctttaaaata
    29161 attagcattt tcccaaactt tttcgacaag ttccggatgt tcttgcatca aagtaatcga
    29221 agccaatgca gctgcagctg cacctggagt tagcgaagta gaaaatagga atggccgact
    29281 tctagctttc aaccagtcga ttagtgtttt cgatccagct acataacccc caacaacacc
    29341 tatagctttc gataacgtgc ccatttgcat atcgattttg tcgcttaatc caaaatgctt
    29401 gacggttcct gcaccttttc cagttacgcc tgagccgtgc gcatcatcga cgtaggtgat
    29461 cagtccatat ttttcaacaa tcggaataat ttccggcaat cgtgcaatgt ctccatccat
    29521 ggagaaaaca ccatctgtga tatacatgat ttttttatat ttcttacttt ctatcgcttc
    29581 ctttgctttt ttctcaaggt ctttcatatc ttgatgttta attcggatga tttttgctcc
    29641 ggataagcgg caaccgtcaa taatagatgc atggtttaat tcatccgata agatagcatc
    29701 ttcttttgtc attactgcag agatagcgcc catattgcag ttgaatccag attgaaaggc
    29761 gattgctgct tctgtccctt tgaattctgc gattttttct tctaattgct gatggatttt
    29821 cagcgaacca ttgatcgttc ggactgctcc tgctcctaca ccataagttt cggttgcttc
    29881 aatacatgct tttttcaatt cttcgcgatt ggcaaatcca agataattgt tggatgctag
    29941 attgatcatc ttttttccgt ctacgatgat acaagcacca ttttcacttt ctagtatgtc
    30001 gatcgtattg tataacccct tttctttcag ttgatccaat tctttctcta aatacattgt
    30061 tagttctttg gaagacattg tctccacctc catattcatc tgtcttgcag aacataccaa
    30121 acatatatcg tgtcattcat ctacaaagca agtatgctat atttttgata acgcttacac
    30181 tgattatgag aattatttaa ccacattttt tatttacagt atctttttac tttcaaatga
    30241 cttatatagt atgaacaatt accttttaga taatgatcta ttttactcta cctgggcaga
    30301 taaatacaac acaaccattg atagaagccc tttcgttaaa atcaattgta ttctgaatat
    30361 ttaatttatt tattgcatag accaataaaa atacataaaa tttccatctt cgtttatcag
    30421 caatttttaa ttttactctc tgaatatttc ttaaaagttt tacttgttga attcgaaaaa
    30481 aggactgtga caaaagtctt gtcacagtcc tattcttccg aataaacggt gttctcctgc
    30541 caattccgaa tcacaagtta caatattcat caaacacgag aagctatttt ttgaaattgt
    30601 tccttgtgac tcggagctga acggcaggct cacgacctct tggtaaacag tgttccagaa
    30661 gtaactttta cggaaaatga tccgaaatat cttggagctg accacttctg tcacaacctc
    30721 catcattttt tgttattttt gatccaattg tctggagtaa aattttcatc gtcaatagac
    30781 cattccagaa aaaattcttc taagaattca cgcatgaagg tttctcttcg tttggcttct
    30841 tcatatccgt aatcagtatt cattttatca gacagcaaga acagtttttc ataaaaatga
    30901 ttgatcactg tcgtgccttt tcggtaattt tctttgtcct tgaatgtttg cggatacagt
    30961 tcaggatcaa aaatcgggtg cccatgtcca ccgccgaaat aagcagtacg caaaatgccg
    31021 attgctccta atgcttcgat acgatcagcg tcttgaacga tcttaccttc cagagatagt
    31081 tcggccgctt cagagagatt tttagaaaag gacatatttt cgataatgga aaaaatcatc
    31141 gagatctgtt cctctactat tggcaaagag cgaaggaata cacgaagctg ttgctttgcc
    31201 tcgttctcat ttttaacgac tttatcatca atcgtatcat gtaggtaagc tgctgcaagc
    31261 gtcacaaagc ggtctgcctg cggttccgtc tccaaaattt tttcagctaa cttgacaact
    31321 cttttggtat gatcgctccc atgtcctgtt tgatcatgcg ctaattggtc catactataa
    31381 tcagcgatca cttgtaattt atccattcgt tccacttctt tcttaagtaa atagctgaaa
    31441 acatctatag attagatgaa tttcagctat ctttgtaaat tgtattttat tttacctgat
    31501 atacattaaa atacaggtct ttcatatagt cgatcaggta ttgcggatta agcggttcat
    31561 gtgttgcatc catgattaat tgatttggtt ttctagatgc accatattga tggatatgct
    31621 ctgacatcca gtttttgatt ggagaataat cgtctgacgc tagaacattt tccacatcta
    31681 tttcttgctg caaagaatga agtaattgtg ccgcatacat gtaccctaat gcataagagg
    31741 gaaaataacc aaagcttgca cctgaccaat gaacgtcttg taggatgcct tccacatcat
    31801 ttgttggtcg tattcccaga tactcttcat atttttgatt ccatatttca ggtagttcag
    31861 caacatctac cccttcattg aaaatcattt tttcgatttc ataacgaatg atgatatgga
    31921 gagggtaagt caaactgtct gcctcgatac gaaccaaact tgattttgta tggttcaatg
    31981 aacgataaaa tttgtcaaaa ggaatatcat cgaatgttcc ttctgcacat tcttgaaaga
    32041 aaggatattg ctttttccag aagctgcgac tacttccaat aatgatctcg ttaaacaacg
    32101 attgtgattc atggataccc attgatgtac cttcataaac cggtgtatac gcatatttat
    32161 catcgatgtc ttgttcatac ataccatgac cagcctcgtg gatcactcca aaaacagcca
    32221 ttttgaaatc tgtttcgttc catcgtgtcg tgattcgtgc atcattcgga ttaattccag
    32281 tcataaatgg gtggacagtg tcatctaaac gaccacgact aaaatcataa ccaagttgtt
    32341 cgattacttt cactacaaat ctcttctgtt gttttttcgt cattctccga tttaaaaagt
    32401 ctgtttcagg ttcagtccct ttttcttgga gcgtacggcg gatcgaaaga attccttctt
    32461 taagctgatc aaatacctta tcaaggattt cgacagtcat cccgggttcg tattgattca
    32521 gcaaaacatc ataattcgtc ttttcatctt ttctccagta aggtatcaac tgttttgtta
    32581 attcaatatt ttgattcaat gcgtgttgaa aatcagaaaa ttgttgtgtt tctctagctt
    32641 tttgccattg tccgtgtgct gaagaaacgg cagctgtata ttttctcata agatcttccg
    32701 gtacgcgatg ttccaaatca tactcttctt ttactttggc aaaaacggca gttccaactt
    32761 ccgacaattc ttccggatgt tcagaaaaat agcgaatggc ttctttgatt tttggaccga
    32821 ttttttttga aaaataaatg ctgtaaagat agctgtctac ttcacttcgg tatgcactag
    32881 ctttttcagg catacctgtt tgtgtatccc aatcgaggat tcctaaagct tgttgtagaa
    32941 gattgatttc ttttacttct tgtaaaaact ctttttcttt cattccctat tcctccaaac
    33001 atccaattga atgaatcaat tagtaagatt attcgtcttt ctcttttttt ctaggcggac
    33061 gagttcccca atactggaat agatccgtac gcaatgcacc attgtacaat tttctttttt
    33121 tggttgcttt ttgtccgtaa aattcctcaa acgctaagtc gctagtcaag atatatttac
    33181 tccatgtttt taatggacgg aatacatcgc ccatttcttt atataatttc cgaacagttt
    33241 cttcttctcc tagacgttct ccgtaaggcg gattagccac gatgaccccg tattctttat
    33301 ccgttttgaa gtctttgact gcttgttgct taaaggtaat cgaagagcct aatccgattt
    33361 cttctgcatt ggctttggca atttcaatca tccgtccatt gatatctgaa ccagtaatat
    33421 ctaattcaat atcataatct gctttttctt cggctgctga tcgaacattt tccatgattt
    33481 cttgactgaa ccaatcccaa gattcgcaag caaactcacg attgaatcct ggtgcgatat
    33541 tatggcctat taaggctgct tcaatacaaa gcgtacctga accacataca ggatcataaa
    33601 atggacggtc ttttcgccaa tttgtcaaca taataagtgc tgctgccatg ttttctttca
    33661 atggtgctcc acctttttct aagcgataac cacgtttgaa taaacttggt cctgtcgtat
    33721 ccagagttac catcacatgg tctttcagca aagctacttc caactgaaaa tgtgcgccag
    33781 tttccgtcag tggtacagaa gctggacggt gataataggt acggagacgt tcaacgattg
    33841 ctttttttgt gatcgcttgg caatcaggtg tgctatatag tttcgacttg atcgattttc
    33901 cagctacagg aaaagctgca tctagtggaa gaaaatcttc ccaaggcaat gcttttacgt
    33961 tttcaaacaa ttcatcaaac gtatacgcat cgaattcccc aaccacgatt tttactcgat
    34021 ctgctgtacg taaccataaa ttagctgtag cgatcgtttc cattgttcct tcaaaacgtg
    34081 cacgtccgtt ctcaacttgg cattcgattc caagatctct taattcttta ccaaccagtg
    34141 cttctagccc gcttgccgca gttgcgacga ggttgaactt cctgtttttt tccatacaca
    34201 atctccttat ttataaaaaa aggaggtggg acatcagtcc taccccacac ttttactcaa
    34261 attccgaata accggtgata caaaagtttg ttttgtaata agaacgattc atctgaagta
    34321 ttgaaaaata gctttcaaat gtttttcttg tttcttataa cgcattactt ttgtcacgac
    34381 ctaattccct gatgtttgcg gttttctata agccatgttc tgtactttgc ttttccaggg
    34441 agaaaaaaca aagcggtaac catctatctg cagttatact gcctttttgt cctgttcttc
    34501 ttccagacaa aaagcgcccc taccattgtt tgggttgctc gctcgagggg tttaccgcgt
    34561 tccaccggat gggtttcccc agccgcttcg tcactgtggc actttcaagg atacttaagc
    34621 atagccgaag ccttagcttg ttttcctgcc gttactccaa agagtacctt agcttatggt
    34681 ttcgctaagc acgaacacta caggcatcac agcctgtgcg agcatggact ttcctcagcc
    34741 tgatcaagtc agaccgcgat tacccgaaaa ccgcaaattc attaatcaaa aattaaaatt
    34801 gacgtgtttt ttcattatca tctaagcttg tgtctgcatt tgtgtagtta tttggattag
    34861 gttgtgctgg tttgactgca cttgcttgtt gatcaagctt tttaccaaat acttcacgtt
    34921 caagatttga caagcgtttt aagatatcaa aattggtcac tgctgcgctc tttggtgcct
    34981 ctgtttgttg cacgcgtgtt tgagctgctc cttgagtttt agataattga gctacttttg
    35041 cactcaatcg atcgttttct tcttgtaaag caaggagttc tttgctgtaa gtttcataat
    35101 ctttgatgat attgtctaag aattcatcaa cttcaacggg atcatagccg cgcatttttg
    35161 ttttgaattc ttgttgtaaa atgtctttag gactataaac caaattcgcc atatttacac
    35221 ctctagtttt catttacgta ataacatttc tacctttttc attgtatagg aaaaaagttg
    35281 ataaatcaaa cacaatatcg attattcact aaaattttga agatcgtcca tactgatcag
    35341 ccgaatttcg tagggatgat ctgcttggta attctgtgca tcttcaaaaa aatacttcgt
    35401 ttttccaggg aattcttcat catagatcag cagacagccc actgtatgct caagtataaa
    35461 acgagtgtga ttctttagct gactaggtga ttggtagggt tgatggctaa cagcttcgac
    35521 ataatctgct aattgttctg ctaattgttt ttttcttggt tcccttcatt ccagttatta
    35581 ccaaaatcag aaaaaggata caaaatacca agagatagtt ccggatattc ttctttcaat
    35641 tccgctacta tttgcacagc ccatagttcg acgcccaaat tcccactgac aagtacccat
    35701 tccagccctt cttctgctaa ctgatggagt tcttttttta aaacattttt aacaacttgg
    35761 attttaggat cattttcttt aaaaacaccg agttcgaaac ttcgataccc agacacatag
    35821 attacttttg acttatccat ctcagattcc ttttctttct atccgctttt ttgctataat
    35881 gtggcctagg agtgtgatta aagatggtat tgcgctatcc aaatggccaa ccttattata
    35941 agaatgcctc tcccgtcgaa aaaattcaag aaaaaagaaa tcagatcact gaattcggca
    36001 atcgggggat gcgctttgaa gaagcaatca atgaaagtaa taaatattat ctttcccgcg
    36061 ggttggcggt tatccataaa aagcctaccc ctatccaaat cgtgaaagta gactatccta
    36121 agagaagtgc tgctgtgatc aaggaagcct actttaccca agcctcaact acagactata
    36181 atggcgtcta tcgaggcttt tacttggact ttgaagcaaa agaaaccaag aacaaaacat
    36241 cttttccctt taaaaacttc catgcccatc aaatcgaaca tatgaaagca tgcctcgaac
    36301 aaaatggggt ctgctttgtc ttgttatggt tttcgtcttt gaagcgctgt ttttatttga
    36361 atagtgttca tttgattgct tactgggacg cccaaaagga aaaaggacgc aaatcactac
    36421 ctttatcttt gatcgaagaa ctaggaatag agatcaaaac tggtatttct ccgcggattc
    36481 cttatttgga cgcggttgac ttctatttat caacattatc tattgaagga gaaagcaaga
    36541 atggcaaatg aacaaactag aagctctaga cgacagaaac agcctactcc taaaaagagt
    36601 gtgaagaaga attctggcaa aggttctgga aagtcctctg gaacacataa aaaaggatta
    36661 tttatcaaga tccttttagg aatactctca tttttctgta tcctcttttt agcaggtgta
    36721 ggacttttct ggtattacgc aaaagatgcg cccgaattga cggataaaaa attagacgct
    36781 accgtatctt ccaaactata tacacaagat ggcgaactat tcgaagattt aggcgcagaa
    36841 aagcgtgaaa aaatttctgc gaatgaatta ccgaaaacat tagaagatgc gatcgtgtcg
    36901 gtagaagatc gccgttttta taaacacatc ggggttgacc ctattcggat catcggttct
    36961 gccctctcta actttacctc tggcggactg caaggcggaa gtactttgac acagcaattg
    37021 atcaaacttt ccttcttctc caccagtgcc gaggatcaga ctctgaaacg caaggcccaa
    37081 gaagcgtgga tggctgtaag attggaacaa aaaaaatcga aacaagagat cttgacctac
    37141 tacgtaaata aagtgtatat gtcaaacggc ttgtacggga tggagacagc ttctgagatg
    37201 tactttggta agaaattatc agaactttct ttaccgcaaa cagcattgct tgctgggatg
    37261 cctcaagccc cttccgccta tgatccttat gtttatccag atcaggcgaa gaaaagacga
    37321 gatactgttt tgtatacgat gcttcaaaac gaaaaaatct ctcaaacaga atatgatcaa
    37381 gctgttaatg taccggtaac ggatggtctg caagaactaa ctcaatccga tgacaatact
    37441 aagatcgtcg ataattatgt caaagaagtc atcaatgaag tacaagaaaa aacagataag
    37501 aatgtctaca cagacggttt agagatttat acgaatttgg atctggacgc ccaaaagaaa
    37561 ctatatgata tcgtcaacac ggatcagtat gtctcttacc cagacgacga gatgcaagtt
    37621 gcctccaccc ttatcgacac caataccggg aaagtaaaag cccagattgg cggacgtcac
    37681 atcgcagaag acgtaacttt aggcaacaac ttagcagtga atacttcccg tgattttggt
    37741 tctacgatga aacctgtcac agattacgga ccagcttttg aatatttgaa atattctact
    37801 ggaaaaacta ttactgacgc accgtataac tatgaaggga cttcgactcc tgtaggaaac
    37861 tgggataatc aatatatggg caccattact ttacgccaag cactttactt atcccgtaat
    37921 gttccagcag tcaaattatt caatgaagtc ggttcagata aagtggcttc tttcttaaaa
    37981 aatcttggga tcgaatacag tacgatccat caatcgaatg cgatctctag taatacggaa
    38041 gaacaagatg ggacaaaata cggagcttct tccttaaaaa tggcagcagc ctatgcggct
    38101 ttcgccaatg gagggactta ttacaaacct caatatgtta ataaaatcgt cttccaagac
    38161 ggaacagaag aaacttatga gccagatgga aaaacagcca tgtctccaga aacagcttat
    38221 atgatcacag acattttaaa agatacgatc actgaaggaa ctggtacgaa tgcgcaaatc
    38281 gctgggttat atcaagccgg taagaccgga acatccaatt acacagatga cgagtatgca
    38341 aaacttggta tttcttctgg ggtgtatcca gatatcctat ttgcgggcta tacgccaaat
    38401 tattcgattt ccgtgtggac aggttacaat aaaaaaatga cacctgtcac ttcagaatcg
    38461 tcacacgtag cctctgatgt ttatcgcgag cttatgcagt atgtttcagc taacgtgaca
    38521 aatacagact gggagatgcc aagcggatta attcgtgtgg gcggtgaact gtactacaaa
    38581 gatcaatata cagctagatc gaatgcaatt acaccatcaa caactattcc atcttccagc
    38641 tatgtccaga ctcctggaag ctcaacaaca gagacaacga ctcaaagctc tagctctact
    38701 agtcaaagcg aatcaactgc tgagtcctca aaagaaagca cgacagcaga aacaagcgag
    38761 ccagcgtctt caacaacggt tccttcttct tcatccgaag aaagctcgac accgtcttca
    38821 tctgctcctc ctgcttcttc cagtgagcca gcaactagcg gagcggatgc tgcaaatgac
    38881 catacaccat ccagcagcac ctcagcttct ggaaacagat agtaaaaaag aaaccacgtc
    38941 tacaagcgtg gtttcttttt tgtgtctatg taaaaaacag ctacaaataa aaatcatgta
    39001 ccaagctgta ttacatgatt ttttataaga ctatccagca tatacatgtg tgatctaaca
    39061 tttagcttaa aaattagaaa atccattttt cttttatcat tacatatact ttatccattt
    39121 acttgtttat aattaaatcg gctaaataca cactagtctt ttatttagtg ccacttctta
    39181 ataatcccat tgttattatc aaataaaaaa tacaatatat ggttttgaat aattttttga
    39241 aagaaggctt cattttgagt tatctgatag tatgcttaat tatcataatt tgtctgcacc
    39301 atcttttttt atcgaaaaca atacctcgtt attccaagaa taaaaaagct gaaaaatttt
    39361 gtttattatt aaataaattt actttaattg ctcctatcct tgcatttatt attttttctg
    39421 tacttttaag cactactctt aaaggaaaat ttatggaaag aagctctcat gcaatgattc
    39481 tcactttttt atggttgttg ttcaccagaa tctatatttt tctgatgagc ctgaaaccgc
    39541 ccaaatccat tagtttatgt ttagttataa atggtatttt tttattatca cttatcattt
    39601 ttattacacc tttagataga tacgtcactt atttatataa tcctttggaa tactggactt
    39661 attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc aataaaaata
    39721 acaactttta tttttatcgc aataaattgt aataagttac catcctagct actgagtatg
    39781 tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat aataacaaga
    39841 taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt ttctttttta
    39901 gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat agcaaaggac
    39961 atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa atcagtgtat
    40021 gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct tctacttcca
    40081 tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc acgtgggtat
    40141 ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct gtctttctgc
    40201 ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca tcgaaacgtt
    40261 caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac aaaccaatcg
    40321 tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg ggtgtcggaa
    40381 aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc tgggcactta
    40441 agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca tgtgcttcgg
    40501 gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt aacatattcc
    40561 gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt tgagctgact
    40621 gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct tccacttgtt
    40681 gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg taattaaaac
    40741 tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc ggctgataat
    40801 ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc gggcgtccaa
    40861 attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt ttttcttcgt
    40921 attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg taaagattat
    40981 atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat ccatttgtta
    41041 cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc atagcaatca
    41101 tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt agaacaaata
    41161 aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga ttagatatga
    41221 ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct ccgctcaatc
    41281 acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat ttacttcact
    41341 gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt tttgatgcgt
    41401 atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag aatgttgaca
    41461 aggaaacaaa attactatac actatttttg tttccaagtt aacattattg cataaaggag
    41521 tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg tctctgtctt
    41581 attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa cgctgcctta
    41641 ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg tttatgctct
    41701 agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg ggcgtcatcc
    41761 gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg gtttaggaaa
    41821 ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag gagaaatctc
    41881 caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca aagtttttgg
    41941 atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag ggggtctatt
    42001 agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt tcttaaatgc
    42061 cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt cagtagattt
    42121 aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga tttcatccgt
    42181 tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc ttcaatctat
    42241 cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg gtttaagctt
    42301 ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc gattgctcaa
    42361 aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc tcgcctatct
    42421 attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg ggattatctg
    42481 ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat ccaattctgt
    42541 ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat ctctatcgcg
    42601 cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc ttccgacggt
    42661 attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat agtagcttca
    42721 tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg tgtcaaattc
    42781 ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt gatggagttg
    42841 ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga tttctttttt
    42901 attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt ttgttagctt
    42961 actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt taggatcagg
    43021 gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg caaattcttt
    43081 ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat attcttctat
    43141 tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat tgtttttttg
    43201 tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa accaag
//
LOCUS       DAFCNS010000015        42902 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFCNS010000015 DAFCNS010000000
VERSION     DAFCNS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.015"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            214..498
                     /locus_tag="LNE23_000791"
     CDS             214..498
                     /locus_tag="LNE23_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBK6930664.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="LNE23_000792"
     CDS             complement(779..1198)
                     /locus_tag="LNE23_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBK6930665.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="LNE23_000793"
     CDS             complement(1200..1784)
                     /locus_tag="LNE23_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBK6930666.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="LNE23_000794"
     CDS             complement(1831..3306)
                     /locus_tag="LNE23_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBK6930667.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="LNE23_000795"
     CDS             3487..3798
                     /locus_tag="LNE23_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930668.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="LNE23_000796"
     CDS             complement(3913..6249)
                     /locus_tag="LNE23_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6930669.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="LNE23_000797"
     CDS             6391..7059
                     /locus_tag="LNE23_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6930670.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="LNE23_000798"
     CDS             7062..8105
                     /locus_tag="LNE23_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6930671.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="LNE23_000799"
     CDS             complement(8342..9223)
                     /locus_tag="LNE23_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6930672.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="LNE23_000800"
     CDS             9382..10110
                     /locus_tag="LNE23_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBK6930673.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="LNE23_000801"
     CDS             10266..10583
                     /locus_tag="LNE23_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6930674.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="LNE23_000802"
     CDS             10576..11184
                     /locus_tag="LNE23_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBK6930675.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="LNE23_000803"
     CDS             11239..12030
                     /locus_tag="LNE23_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBK6930676.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="LNE23_000804"
     CDS             12168..12656
                     /locus_tag="LNE23_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBK6930677.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="LNE23_000805"
     CDS             12707..13579
                     /locus_tag="LNE23_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK6930678.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="LNE23_000806"
     CDS             13755..14117
                     /locus_tag="LNE23_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6930679.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="LNE23_000807"
     CDS             14126..14878
                     /locus_tag="LNE23_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6930680.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="LNE23_000808"
     CDS             14892..16694
                     /locus_tag="LNE23_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6930681.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="LNE23_000809"
     CDS             16971..18011
                     /locus_tag="LNE23_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK6930682.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="LNE23_000810"
     CDS             complement(18063..20207)
                     /locus_tag="LNE23_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK6930683.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="LNE23_000811"
     CDS             complement(20564..21937)
                     /locus_tag="LNE23_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6930684.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="LNE23_000812"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="LNE23_000812"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBK6930685.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="LNE23_000813"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="LNE23_000813"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBK6930686.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="LNE23_000814"
     CDS             complement(23385..24398)
                     /locus_tag="LNE23_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK6930687.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="LNE23_000815"
     CDS             complement(24479..25078)
                     /locus_tag="LNE23_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6930688.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="LNE23_000816"
     CDS             25264..25965
                     /locus_tag="LNE23_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6930689.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="LNE23_000817"
     CDS             25975..28686
                     /locus_tag="LNE23_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6930690.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="LNE23_000818"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="LNE23_000818"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBK6930691.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="LNE23_000819"
     CDS             complement(30825..32006)
                     /locus_tag="LNE23_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6930692.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..33781
                     /locus_tag="LNE23_000820"
     CDS             32258..33781
                     /locus_tag="LNE23_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBK6930693.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33850..34578)
                     /locus_tag="LNE23_000821"
     CDS             complement(33850..34578)
                     /locus_tag="LNE23_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6930694.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34591..35871)
                     /locus_tag="LNE23_000822"
     CDS             complement(34591..35871)
                     /locus_tag="LNE23_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK6930695.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36012..36614)
                     /locus_tag="LNE23_000823"
     CDS             complement(36012..36614)
                     /locus_tag="LNE23_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK6930696.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36859..37065
                     /locus_tag="LNE23_000824"
     CDS             36859..37065
                     /locus_tag="LNE23_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930697.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37377..38096
                     /locus_tag="LNE23_000825"
     CDS             37377..38096
                     /locus_tag="LNE23_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK6930698.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38177..38860)
                     /locus_tag="LNE23_000826"
     CDS             complement(38177..38860)
                     /locus_tag="LNE23_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBK6930699.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39078..41264
                     /locus_tag="LNE23_000827"
     CDS             39078..41264
                     /locus_tag="LNE23_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK6930700.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            41610..42020
                     /gene="spx"
                     /locus_tag="LNE23_000828"
     CDS             41610..42020
                     /gene="spx"
                     /locus_tag="LNE23_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK6930701.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            42034..42390
                     /locus_tag="LNE23_000829"
     CDS             42034..42390
                     /locus_tag="LNE23_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBK6930702.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        13982 a         7547 c         8578 g        12795 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg agaattggcc
    33721 aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc ttttgcactg
    33781 ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt
    33841 cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc
    33901 gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat
    33961 aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt
    34021 ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag
    34081 cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc
    34141 tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag
    34201 gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg
    34261 ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat
    34321 ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact
    34381 gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc
    34441 attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat
    34501 catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca
    34561 aatataaatg ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga
    34621 aaaacgctgg ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc
    34681 atccaagata aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt
    34741 ttgtttcaac tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac
    34801 ggaatgattc ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc
    34861 ttcaatcgca ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt
    34921 atccagttcg attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat
    34981 catgagtttt aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact
    35041 tagattggcc ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata
    35101 agaccgtaat ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt
    35161 ttggtaatca tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata
    35221 atcctgttgc atcctgactt gttgctctaa taactgatca gctaatttct tattagagat
    35281 tttccaataa taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt
    35341 attcaaagct aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata
    35401 gtatgtaaat aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa
    35461 ccaagtctct aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa
    35521 gacaatgtga aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa
    35581 aaaatcgccg atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag
    35641 cgcgtaaacc aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc
    35701 tatagcaatt agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca
    35761 agaaatcaga atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa
    35821 gatacaaatt gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag
    35881 ctcccattga tttttaccaa attatagcac aattggcgta ttattttttt aatttttcag
    35941 ggattttgaa ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt
    36001 tagttaattg tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag
    36061 tttgtaatag gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa
    36121 aagtaagaag ctgagaaaaa taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc
    36181 ttctttaata atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat
    36241 ccatagcgaa gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag
    36301 atagtcaatt gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac
    36361 agcagacaaa cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata
    36421 taagaaagaa aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga
    36481 acgaccgcta gttttccaag attattcaat aaaacttcta agacaaattt tacttggaca
    36541 tacaccatat cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca
    36601 actttatctt tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa
    36661 acgtcttttg tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa
    36721 atcttcgttt tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt
    36781 ttatgacaag tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa
    36841 ggtgatggag ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca
    36901 tttaaatctg atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat
    36961 agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg
    37021 cactggcagt tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt
    37081 tgagagcata aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta
    37141 aaaaacagct tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat
    37201 actgtttatt tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa
    37261 taggagttac ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg
    37321 cttttatttc tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc
    37381 cgataattgt gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa
    37441 tgcttgccca agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat
    37501 tgccgctatt ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga
    37561 acaaacgctt tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct
    37621 ccatctacga agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag
    37681 tagaagtaaa gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt
    37741 cgccacaaaa gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag
    37801 cgagaatcaa aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt
    37861 cctactataa agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc
    37921 ccaaaatagt gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac
    37981 aaatcgtaca agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt
    38041 cgatccgcta cactacaaat aacccgaaag caaagggaat ctttacaact aaataatata
    38101 aaaactaagc aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt
    38161 ttttgattca ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa
    38221 atgtctgagt gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc
    38281 caaacagatg atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac
    38341 ttagatagtg gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata
    38401 agttcgttgc tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt
    38461 tgattttatc aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta
    38521 atgcgggatg ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat
    38581 atagaacaaa caagcgccag aaatgttttt ctttttttct aaaataatca attagatact
    38641 ttgttagaag aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta
    38701 gccattctcc gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg
    38761 caaaagcacc atattggata taagtataac tgtccatggc tcctccgata aatgtcagca
    38821 aaagtcctat tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg
    38881 aatatttttg tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa
    38941 aactaaaatc atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa
    39001 aatagacaac gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg
    39061 ggaaggaaat caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac
    39121 gctgggagaa caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg
    39181 ctacctatga agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa
    39241 aagaaagtga aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct
    39301 tcaatcaaga acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag
    39361 caggagctgg gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc
    39421 aaaaacaagt agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg
    39481 aactaaaaaa acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat
    39541 tccatagtat cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa
    39601 tgcaaggaat acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc
    39661 aacagctgat acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg
    39721 cttctcggtt agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag
    39781 ttaaagaaaa atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt
    39841 atggattgct cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg
    39901 atgtactcca aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc
    39961 aaaaatatga ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa
    40021 tcgtgaaacg gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc
    40081 agagtatcta tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat
    40141 atcctaccaa aactcttttc ttattaacta attatcgttc tcgggcagct attgtccaag
    40201 gagcaaatag attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag
    40261 cacaaaaaga agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc
    40321 agtggctaat caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa
    40381 tcatattata ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga
    40441 atattccttt tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt
    40501 tccaacattt ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa
    40561 atcaggccta ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg
    40621 aatgcgatca atacttttct cctaaatatc aaaatatagc cgttgcagat tatattttgt
    40681 cgataagccc caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc
    40741 gtaaaacaga ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac
    40801 gtatagctaa ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc
    40861 atgctacttt cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat
    40921 taaaaacccg cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca
    40981 ttcacaaaag taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa
    41041 gtttgcctta taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag
    41101 aaccagtaac gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa
    41161 gaaatcgtct gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga
    41221 gatttatcaa agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg
    41281 acagaagtgg tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt
    41341 tgtgaaattt cagcttattt tcgaagaagc tatttctgga acacagttta ttcggaaaag
    41401 ggaggtgtga caagaatttt gtcacacctc ccttatttat tttgaaaatg agaatactat
    41461 tatcaaaata gagaatatat agaaaaattc tcattattca aacgtaaata atcagttatt
    41521 ttgatttttt ttatatttta ttagctaata attggtattt taaaagaaat tactgtagaa
    41581 ttggattata attaaaaagg agtgagaaaa tgataaaagt atatacttcg cctagctgta
    41641 cttcctgtag aaaagcaaga gcatggttgc aagcgaatca acttgaattt gaagagaaga
    41701 atatttttgc cgaaccgtta acagaaagtg agatcaaaaa gatcttggca gcgactgaag
    41761 gaggagttca agatattatt tccactcggt cgaaaattta cgaaaagctg ggcatagatt
    41821 ttaacgaatt aactttaaag caaatgatcg tcttattcaa agaatatcct tcattattga
    41881 gacgacctat tttgattgat gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc
    41941 ggtcatttgt ccctcgtgaa attcgatgtg ttgccagaaa tctgatctat ttacaaaaca
    42001 ataacatctt aaattcatag ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg
    42061 cactgtgtaa caagtctgaa ggcgaaacaa tggttagaac accatcaatt gccttatcaa
    42121 tttgttgact tagaagaacg cgatctaagt acagaagaag cagaagaatt atgcagtttt
    42181 gaagaagtgc aagcagaaca gctatttgcg acttggtctg aggcttttca caacattgcg
    42241 attgaccttt cctgtgtagg aaaaaaacaa ctgatttttt tatgcacaca acataccaat
    42301 ctcctcagac gtcctttgat tattatcgat gaagttctat ttattggata caatgagcag
    42361 ttgatggaac agcttatctt aaataaatag actttaactg ttttaatcaa gtcactgagc
    42421 atttcataca gttgtaacag tcgaacactt agcatcaagc gtgttatgtg ttcttttttt
    42481 gactttattt ttctactata gagacatgct caatttatgt ggtcaaaatt caaatctagg
    42541 tggggatata gagttaaaaa tatattgaca tgtgtaaatt tacatgatat attcatttct
    42601 gttgttttaa gtagcaaagc aataaaatag tattttaaaa aacatataaa atagttgttg
    42661 acatgttaga aatgacatga taagataact gagttgttaa aaaagaacca cttgtgaagc
    42721 ttgattctat cgaaaaaaac ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg
    42781 ataaatgagt tgctgtaaga tttcgaaaaa cgaaatcaaa aaaactttta aaaaagagtt
    42841 gacaattact tgtctctctg atatgatata aaagttgcta cggcagccaa aaacgaatcg
    42901 gg
//
LOCUS       DAFCNS010000016        42686 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFCNS010000016 DAFCNS010000000
VERSION     DAFCNS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.016"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            109..2550
                     /locus_tag="LNE23_000830"
     CDS             109..2550
                     /locus_tag="LNE23_000830"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBK6930703.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2880..3584)
                     /locus_tag="LNE23_000831"
     CDS             complement(2880..3584)
                     /locus_tag="LNE23_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930704.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3771..4016)
                     /locus_tag="LNE23_000832"
     CDS             complement(3771..4016)
                     /locus_tag="LNE23_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930705.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4033..4884)
                     /locus_tag="LNE23_000833"
     CDS             complement(4033..4884)
                     /locus_tag="LNE23_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6930706.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5050..6054
                     /locus_tag="LNE23_000834"
     CDS             5050..6054
                     /locus_tag="LNE23_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBK6930707.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6295..6651)
                     /locus_tag="LNE23_000835"
     CDS             complement(6295..6651)
                     /locus_tag="LNE23_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6930708.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7019..8206
                     /gene="tuf"
                     /locus_tag="LNE23_000836"
     CDS             7019..8206
                     /gene="tuf"
                     /locus_tag="LNE23_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK6930709.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8394..10985)
                     /gene="mprF"
                     /locus_tag="LNE23_000837"
     CDS             complement(8394..10985)
                     /gene="mprF"
                     /locus_tag="LNE23_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK6930710.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11011..11772)
                     /locus_tag="LNE23_000838"
     CDS             complement(11011..11772)
                     /locus_tag="LNE23_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6930711.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11939..12571
                     /locus_tag="LNE23_000839"
     CDS             11939..12571
                     /locus_tag="LNE23_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBK6930712.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            12630..13490
                     /locus_tag="LNE23_000840"
     CDS             12630..13490
                     /locus_tag="LNE23_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBK6930713.1"
                     /translation="MEKRQKMENQKVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13675..15990
                     /locus_tag="LNE23_000841"
     CDS             13675..15990
                     /locus_tag="LNE23_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6930714.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16172..18013
                     /locus_tag="LNE23_000842"
     CDS             16172..18013
                     /locus_tag="LNE23_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBK6930715.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18037..19146
                     /gene="rpoD"
                     /locus_tag="LNE23_000843"
     CDS             18037..19146
                     /gene="rpoD"
                     /locus_tag="LNE23_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBK6930716.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19363..21504
                     /locus_tag="LNE23_000844"
     CDS             19363..21504
                     /locus_tag="LNE23_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK6930717.1"
                     /translation="MMKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDA
                     AMQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIE
                     KNEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHRED
                     TKEEHAKELLKMISEKIAQENKVEPEHEAEKDTEKDISGVNAAGKESLKTNDLKTNDL
                     KTDIPEKTETLSEKQSEDEKSNGLPEADKKEMLEKADKKATESDGINNEADAETQSAS
                     EDSTALEAKIEKEENQPELIEAETEPITIVPNTEKTPQESNPEKKDLDKTQKKKSKKV
                     PYLALAAVLLLGGGGWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFI
                     RADMIHQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDH
                     LADDIKLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVV
                     DSATRDQYKTAKEAVEKVKNTDLSAQLNEQLQQVDKMLTDKEKKEEEEKKKAEEAKKA
                     EKAKKAAEEQAKQAAQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARAS
                     DIADASNPAWTWAPGVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKS
                     ALMKGIGESALPFYIVTINCKTGWFGGNGSN"
     gene            21700..22563
                     /locus_tag="LNE23_000845"
     CDS             21700..22563
                     /locus_tag="LNE23_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK6930718.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22668..23141
                     /locus_tag="LNE23_000846"
     CDS             22668..23141
                     /locus_tag="LNE23_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK6930719.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            23237..24124
                     /gene="xerD"
                     /locus_tag="LNE23_000847"
     CDS             23237..24124
                     /gene="xerD"
                     /locus_tag="LNE23_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBK6930720.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            24143..24514
                     /locus_tag="LNE23_000848"
     CDS             24143..24514
                     /locus_tag="LNE23_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK6930721.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGVYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            24484..25281
                     /locus_tag="LNE23_000849"
     CDS             24484..25281
                     /locus_tag="LNE23_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBK6930722.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            25265..25834
                     /gene="scpB"
                     /locus_tag="LNE23_000850"
     CDS             25265..25834
                     /gene="scpB"
                     /locus_tag="LNE23_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBK6930723.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISFEEDVSPDLFFDGMNKGE"
     gene            25840..26556
                     /locus_tag="LNE23_000851"
     CDS             25840..26556
                     /locus_tag="LNE23_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK6930724.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      26638..26762
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            26885..27496
                     /locus_tag="LNE23_000852"
     CDS             26885..27496
                     /locus_tag="LNE23_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6930725.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIP
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            27874..29397
                     /locus_tag="LNE23_000853"
     CDS             27874..29397
                     /locus_tag="LNE23_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBK6930726.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            29634..30974
                     /locus_tag="LNE23_000854"
     CDS             29634..30974
                     /locus_tag="LNE23_000854"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBK6930727.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            31128..31865
                     /locus_tag="LNE23_000855"
     CDS             31128..31865
                     /locus_tag="LNE23_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6930728.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGNSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(32030..32251)
                     /locus_tag="LNE23_000856"
     CDS             complement(32030..32251)
                     /locus_tag="LNE23_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBK6930729.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            32282..33313
                     /locus_tag="LNE23_000857"
     CDS             32282..33313
                     /locus_tag="LNE23_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930730.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYERILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            33300..34703
                     /locus_tag="LNE23_000858"
     CDS             33300..34703
                     /locus_tag="LNE23_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBK6930731.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGFLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVIHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKHQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            34769..35404
                     /locus_tag="LNE23_000859"
     CDS             34769..35404
                     /locus_tag="LNE23_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6930732.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            35453..36133
                     /locus_tag="LNE23_000860"
     CDS             35453..36133
                     /locus_tag="LNE23_000860"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBK6930733.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAIQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            36268..37497
                     /gene="rpsA"
                     /locus_tag="LNE23_000861"
     CDS             36268..37497
                     /gene="rpsA"
                     /locus_tag="LNE23_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBK6930734.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            37679..38989
                     /gene="der"
                     /locus_tag="LNE23_000862"
     CDS             37679..38989
                     /gene="der"
                     /locus_tag="LNE23_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBK6930735.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            39232..39507
                     /locus_tag="LNE23_000863"
     CDS             39232..39507
                     /locus_tag="LNE23_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBK6930736.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            39742..40788
                     /locus_tag="LNE23_000864"
     CDS             39742..40788
                     /locus_tag="LNE23_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930737.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQRPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            40790..42049
                     /locus_tag="LNE23_000865"
     CDS             40790..42049
                     /locus_tag="LNE23_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK6930738.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIAPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            42055..42636
                     /locus_tag="LNE23_000866"
     CDS             42055..42636
                     /locus_tag="LNE23_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBK6930739.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
BASE COUNT        14355 a         7344 c         9028 g        11959 t
ORIGIN      
        1 gagaatattt aaggattgtt aaaatttaat cttagaaaaa aacaactgga acaagagcta
       61 tttgttttta cagcaaagaa tggtatattt ttgtaggagg tggcttactt gaaagaagtc
      121 gttatgatag atgctgcaag aacgccgata ggaaagtatc gcggaagttt aagtccattt
      181 actgcagttg aattaggcac acttgtgact aaaggattat tagataaaac aaaactgaaa
      241 aaagataaaa tcgatcaagt aatattcgga aatgtcctgc aagctggtaa tggtcaaaat
      301 gttgccagac agattgcttt gaacagcggt ctccctgtag atgtcccagc aatgacgatc
      361 aatgaagttt gcggatctgg tatgaaagca gtgatcttag cacgccagct gatccaatta
      421 ggtgaagcag aacttgtgat agccggcgga acagagagta tgtctcaagc acctatgctg
      481 aaaccgtatc agtcagaaac aaatgaatat ggtgaaccaa tttccagtat ggtcaacgac
      541 ggattgactg acgcattttc aaatgcacat atgggattaa ccgcagagaa ggttgcaaca
      601 caattttctg tgagcagaga agaacaggat cgctatgcct tgtcgtccca gttgaaagca
      661 gcacatgctg tcgaagccgg tgtattttct gaggagatca tcccagtcaa gatttctgat
      721 gaagacgtgt tatctgagga tgaagcagtt cgtggaaata gtacattgga aaaactgggc
      781 acgttacgta cagtattctc agaagaagga actgtaacag caggaaatgc ttcgccattg
      841 aatgacggtg cctctgtggt gatccttgca tccaaagaat acgcagaaaa taataatctg
      901 ccttatttag caaccatcaa agaagtagcg gaggtcggta ttgacccaag tatcatggga
      961 atcgctccaa tcaaagccat tcaaaaattg acagatagat caggaatgaa tttatcaaca
     1021 atcgatcttt ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt tagccaagaa
     1081 ctgcagttag atgaagaaaa ggtcaatata tatggaggag caatcgcatt aggccatcca
     1141 attggtgcaa gcggtgcacg tatcttgact acattagctt acggattgct tcgtgaacag
     1201 aagagatacg gtattgcttc attatgtatt ggtggaggtt taggcttggc agtcttgtta
     1261 gaagcgaata tggagcaaac acataaggat gtgcaaaaaa aaaagtttta tcagcttacg
     1321 ccatctgaac ggcgcagtca attaatagaa aaaaatgtat tgacacaaga aactgcgttg
     1381 atttttcagg aacaaacact ttctgaagag ctctccgatc acatgatcga aaaccaagtg
     1441 agtgaagtgg aaatcccaat gggcatcgcg cagaattttc aaatcaatgg taagaaaaaa
     1501 tggattccga tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa tggtgcaaaa
     1561 atctgtggaa atatctgcgc agaaacacct cagcggctga tgcgagggca aatcgttcta
     1621 tcaggaaaat cggagtatca agcagtgatc aatgcagtta atcatcgaaa agaagagctg
     1681 attctttgtg caaatgaaag ttatccttca atcgtgaaac gcgggggcgg tgttcaagat
     1741 atctctactc gggaattcat ggggagtttc catgcatact tatctattga ttttttagtg
     1801 gatgtaaaag atgcaatggg cgctaacatg atcaactcga ttcttgagag tgtggcaaac
     1861 aagttacggg agtggttccc tgaagaagag attctattca gtattttaag taattttgct
     1921 acagaatcat tggcttctgc ctgttgtgaa atcccattcg aacgcttagg gagaaacaaa
     1981 gaaatcggtg aacaaattgc caaaaaaatc caacaggcag gggaatatgc gaagcttgac
     2041 ccttaccgtg cagcaacgca caacaaaggg atcatgaatg gtatagaagc tgtagtggca
     2101 gctacaggaa atgatacacg tgcggttagc gcttcgattc acgcctacgc ggcaagaaat
     2161 ggattatacc aaggattaac agactggcag atcaaagggg ataaactagt tggaaaacta
     2221 acggtgcctt tggctgttgc cacagtaggc ggagcttcca atatcctgcc aaaagcaaaa
     2281 gcatctttag ctatgttaga tattgattca gccaaggaac tagcacaagt aattgctgca
     2341 gtagggttag ctcaaaacct ggctgctttg cgtgcattag ttacagaagg aatccaaaaa
     2401 ggccacatgg gacttcaagc tcgttcatta gcgatctcaa ttggggctat tggtgaggaa
     2461 atcgagcaag ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca gacagctata
     2521 caaatattgg aaaaaatccg ggaaaaataa aaaagcaata tctttagaag aataaagcaa
     2581 gatttacagt tattttattt actgcaaatc ttgctttttt ctctatcaga aaatatgttt
     2641 attattagaa ttttttatag ttaaggttag gcatcttaag aaatcaggca actccaagat
     2701 atcttcaggt agaagagaag tcgcctaaca ttgtaggatc ataggtgatt tgttggacag
     2761 gtcaagctga tatttgagcc tgatgttaga aggaatttct ttttacttgc tgtagaaatt
     2821 attatatgac taaaaaggct gggattttaa atcccagcct tttatgacaa atagaatttt
     2881 tatcgatcag taatcgctgt cgctgcaaat tgttcgatca aaggaatcaa agtcaatttg
     2941 actttgtatt tccgttcgag tgctttttcg ataacgaact cattttcagt agtcatagaa
     3001 atagttgcta atttacgagg ttcatcagca tttttttcat taataactac ttcatgttca
     3061 ttttgttctg tgtaaaattt aggatactca gcaacggctt ttgaaaaatc agcaaaactt
     3121 aaagcggcat tcgtatcagt tgagcgtgta ttttcagttg tgtccgcttt tggtttttcc
     3181 gttttttctt ctggcacact gactgtttct tctgtaactg gtgtcgcaac agcttgcgca
     3241 gttggttcat cgtttttcaa tcgttgatca ttccagtaag acacgatttc aataagaaga
     3301 ttgtcacgga atcgttcgaa aatcccgttt tctcctgaac gaattctctt taatacagta
     3361 ttgaagttgt catcgattgg cacgttgatc cgtgtatctt catttgtttt cccagctaat
     3421 ccgatcgtta agatttgacg gtttttggga tcaagcatga cttgtaacgg caaaccatag
     3481 cgtcgcaagc tctcttcttc tagagaacgg aataattgtc ctcttacacc atccaatgaa
     3541 ggacggaatt gatttaataa tttttgtttc tgttttttct tcatatgttc tctccttcgc
     3601 tcataccctt tcgattataa gaaaaaaaca gccaaatgaa aaggagtaat gcaaaaaaac
     3661 ttgcgtatac gcaaattaca acagaaaaat ttgcgtattt tcccgttttt tgaaagaaaa
     3721 ataaaaaatg gaacggatta tgtatatata cataatccgt tccattcttt ctattttttc
     3781 ggtatcaagg caaaaacagg tagttcaggt gaataaatga cttcatgttc gtgccagggt
     3841 ttgaccatta gcgaatgatc gaaccgtttt ttcagttctt cgatgatttg atcatgtgtc
     3901 cagtttcttg caggaaaatg ttggattttc tctgcatcga tcaaaaagac aaaatcggaa
     3961 tgaaaagtct gggtaacttc ttccgagaga gaattcaagc gttcgattgg atccataaaa
     4021 tcacctttcg acttaaaatt gggtagtatc tgggtctttt gctttgccga ctacaccatc
     4081 taactgatcg atttgtttca tttcttcttc tgttaattca aaatcaaata attcagtatt
     4141 ttctttgatt ctgcttggtg tcactgactt aggaagagga aggaattcgt gttgcagcga
     4201 ccaacgcaaa gcaacttgtg caatcgtctt gtcatgtgct tcagcgattt gtttcatttc
     4261 aggcacatcg aagattttac cagtgcctaa tggactatat gcttcgagga tcatatcatg
     4321 tttttttgca tactcaacta cagttggctg taattcgcct ggtgcaagga aaacttgatt
     4381 gaccatcggc ataatttttg ctgtttttgc tagtgtatct agatggtggg gtaagaaatt
     4441 gctcacaccg attgctttga ttttacctgc ttcatacagt tcttcaaatg ctcgccatgt
     4501 atctgcattt gcttgttccc aattatcacg aaaagcaaca gggtttggcc agtggatcaa
     4561 aaacagatct aaataatctg tttggagctt cttcaatgat tcttcaaaag aagacatcac
     4621 aagttcatag ctgtggtttg cattccataa ttttgttgtc aagaatatct cttctctagg
     4681 aattccactt tccttaatcg cttggccgac actttcttca ttcttgtatc cttgggcagt
     4741 atcgatatgg cgataacctg cagccagcgc ttctttaaca gaggaaacag cgacatcccc
     4801 atcaggtgtt tgccatgtgc cgaatcctac taccgggatt tcgtaaccat ttaatagacg
     4861 ataagtgctt gttaatgaat tcatactttc agctcctttt ttattcgttc aactataagt
     4921 gtagcgcatt tttcgaaaaa aagaatccat tttgtttcta ctcttgaaat ttttgtttaa
     4981 aagaaaatga ttttgattct ggtatttttg attttttttc cgtaaactaa gacagaaggg
     5041 aatgagaaaa tggatatcta tttaaaaaaa gcaattctgc atattattga cagagaaact
     5101 ggttctccag tcttttcaca aaaagagctg gatctgacaa aagaatatat tcgtgatttt
     5161 cttcaaaaga agatccaaaa aatctcttct gctcaaacaa aaactggaca actagcagaa
     5221 gaaagcatga tcgttgaatc acttaaaatc gctcaagaaa attttgtgga agcaagtgaa
     5281 gcaatcgttc agcggtggtt cgatatctac caagaaagtg aagaagcacc tagtgcagat
     5341 gtatttgttg ttttgtatga attagataca gtgatgtact tatctttatt gaaagtcaat
     5401 tatcgtgagg cttacactca ctttgttgaa gcagacgaag cagggatcga caaccgcttg
     5461 attttgaatc gagcaattct aggggcgaaa acacaaaaag cagatgaagc attcgctgtg
     5521 aatttgactg attggaccta cgaattaatt gaaaaaaaat atgagttttc tggcaaaaaa
     5581 gagctttatt tttctggaag agtgatcgaa agtgttcctg caccttcttt agaagaaaat
     5641 gtaaaagtga tccaaaaagt agccaagcat cttggaaaaa aatttgagac agaagaattt
     5701 gatatcatgg ctgatttgaa agaagctgtc tatgatacga tagaagaaaa aggtcggttg
     5761 gatcatgaga tgattgcgga aaaagtcttc aaggaaaaca tcacggcaaa aatggctttt
     5821 aaagaagaag tacaagagca aggatttgtc ccagaagcac ctcctgtaaa agaagtacaa
     5881 gaaatttctg agaaaaaatt tggaaaacaa aaattcaagc tatcaaacgg gatcgaactg
     5941 atcgtaccag tagatgtata tcgcaaccca gatttgattg aatttgtgaa taatccggat
     6001 ggtacgatat ccgtgatgat caaaaatgtg gatgaagtat tgaatcgttt ataaaaatga
     6061 ctagaacatt aggtgttcaa gtaccagtga tcagttaaat gaattgtgtc aagagaagga
     6121 tcggaaaaga cgaatataac aaaaaaagag cgtgagacaa aagtaaagaa cacttttgtc
     6181 tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttctta tttcttggag
     6241 ttaaacgctt ctgtcccagc ctctttttat ctaataaaat tttaagacca tttactaatt
     6301 atcgattttt tctgccgcat ttttaggcac ttcagaagcc tttatttctt cccagcttaa
     6361 tacaccttta cgaggattga ctttgagttt aagatacgca ttcatcttta atggatgttc
     6421 tctttcttcg cgaagcgtca cctgtttttt ttcaccatct ttattataag ccgcttgctt
     6481 gtaggtataa gtcgtgaatt tctctccgtt atctgcttgg gtagctgaca tttcgccatt
     6541 cgtcgtgatt tttgtataat aagcttcgcc gccgtaaaaa taatgataag ctgcaaaact
     6601 tccgccgaga aatatgccta aaacgacaag tacgccaatt aattttttca tgtacttcac
     6661 ctctttcctt attattaacg aaatagaaga tttcgtctat tgaaagttgt tggcaacagc
     6721 tgaattttaa acttttcctc attttgttct tattgcttca aaagaaagtt aaggactatc
     6781 ttgaagtttt tctcaaaaaa cacatttttt tatttataaa aggtactttt ttcgcgtata
     6841 agacaagcga gtgctcttag ttgactttga ccaatattga cgtataataa gcttagttcg
     6901 aatcttacaa aaagtaagtc ggacgtattc tttttgattt agtcaaaaaa agactataat
     6961 aaaaaggtga gagctgaaat ttttcagtga tctatattat ttaggaggtt tgtaaattat
     7021 ggcaaaagaa cattatgaca gaagtaaacc acacgttaac attggtacga ttggacacgt
     7081 tgaccatggg aaaactactt taactgctgc gatcactaca gtattaggca aaaaaggcct
     7141 agcaaatcct caagactatg ctagtattga tgcagctcca gaagaacgtg aacgtgggat
     7201 tacgatcaat accgcacacg tagaatatga aacagaaaaa cgccactacg cgcatattga
     7261 tgcaccagga cacgcggact atgtgaaaaa catgattaca ggggcagcac aaatggatgg
     7321 tgcaatctta gttgtttctg caactgacgg tccgatgcct caaacacgtg aacacatttt
     7381 attgtcacgc caagttggtg taaaatacct gattgttttc ttgaacaaag ttgatttagt
     7441 cgatgatgaa gaattgatcg atttggtaga aatggaagtt cgcgagttat tgagcgaata
     7501 tggtttccca ggcgatgaca ctcctgtgat caaaggttcc gcattaaaag cattgcaagg
     7561 cgatccagat gctgaagctg ctattatgga attgatggat acagtagatg aatatatccc
     7621 aacaccagaa cgtgatacag ataaaccatt actattgcca gtggaagacg tcttctcaat
     7681 tacaggtcga ggaactgttg cctcaggtcg tattgatcgt ggtgctgttc gtgtcggtga
     7741 tgaggtagag atcgtaggga tcaaacctga aacacaaaaa gcagttgtaa caggtgtaga
     7801 aatgttccgt aaaacgttag attacgggga agctggggat aacgtaggcg tgttgttacg
     7861 ggggatccaa cgtgacgata tcgaacgtgg acaagtgctt gctaaaccag gttccattac
     7921 tccacataca aaattcaaag cagaagtgta cgtgttgaca aaagaagaag gtggacgtca
     7981 tactccattc ttcaacaact atcgtccaca attctacttc cgcacaactg atgttacagg
     8041 aacaatcaca ttgccagaag atacagaaat ggtcatgcct ggtgacaacg tgacgattga
     8101 tgttgacttg atccatccaa tcgctgttga aaacggaacg acattctcaa ttcgtgaagg
     8161 cggacggaca gtgggttcag gtatcgttac agagatcgaa gcataagaaa cgcgactcga
     8221 atattttggt aatgaaaaat gtttaaaata aaagagcggt ccaaacagga tttctgtttg
     8281 gaccgctctt tgttcttcaa ttgtataaga agagattgta gaagcatgaa ctttcgtttt
     8341 acttcagtct cttttattca tcatgagagt gttcatctat cggtatgcag tttttaatac
     8401 tttcttcgta tccatttttt caaaccactt attctacctt gtgtctgctc aactggcgca
     8461 ttatcaataa tcagcaaagc aatcataaca tatgcaatcc aactatctct agaatataga
     8521 gtataacgtg agacccagtt tgtagcgtac ttgcttttat attctcttaa tccatggaaa
     8581 gaataaaaat gagaaccaaa ctcgtaaacc aagtatgcaa tcctttcctg taaaaaactc
     8641 tttctggaag ttccgacatt tgaaagaggg gccatcccta aattgaacca ttggatatgt
     8701 tcttctttca tataggcaaa caaatgcaag aacaagaaat ccatactgcc agaaggtgct
     8761 tttttaggat catatcgcat cagatcaatc gttcctactt tattgttcgt gtaagtaggc
     8821 atgatcgttg caaaagcgac aatttcttct tgggtatttt tgacaactgc aataggcgct
     8881 ctttgtagat aatcttcaga gaaaaagcct aaagaaaacc ctttttcctt tcgtgaaccc
     8941 aaccaattat cagagatatt tttgaaagta ctcatttgct cagtagaaaa tggtggttgt
     9001 aagacatcaa atgtaaatcc ttctcgttcg atccgattca agacagcacg agtgcccttc
     9061 atttttttcc ctgaagttgt aaaactattc aggtctacat aagcttcttc ccccatctta
     9121 atgaaatcgt agccgaattc atgcaaaatc ataacaatct cttcgcttgt ttcgtagaaa
     9181 actggtaaat agcatagtcg atctgtttct tcgataaaag cttcgattgc ttctggaaaa
     9241 tcttctttct taccagaagg atcacccatt acaatacatt tattattgaa gcaagcgaat
     9301 tggagaagaa cagttggttc accgtctttt tcataggcaa acatccgctt atcttttaag
     9361 aagatcaatt gactatcgga gtttcctcca taagttgtaa ggatcttcag ggccttttct
     9421 tcattaaagg cttcgccaat ctgtttcttt tctccttgaa ggaaatgaac aaatagaacg
     9481 atcatgaaac taacagcgat gatggccaac aatcccgaaa accaaatctt ttcagaagga
     9541 aataagaaaa aggaaataaa atggtgacgc cgatgaggga aatccggcag attgtatacc
     9601 ccaataacga tgtaaagcaa ggacagtgca ccaataatga ttccatcgat cgtacgccac
     9661 tcccatgaat agatcaattg ttcccgaaat aattcgtttt tagaagcaag aatgatcaga
     9721 agcagaatag acaagaaaac caccggtgtg aaactaaaat cacttaataa aacataaagt
     9781 aaagctagaa caatcaagaa gattgtaggc aaatatgctc gtttcacacg tgctgcgatc
     9841 cctctgccca taatgattaa aagaaatccc aatagtatac taggaaattg gataataaaa
     9901 tgaaatttca atgggttcaa actatgaagc cagcgaaact ctgtaaaagc ttgcgggata
     9961 gtggcagaaa gtactagcat gattccagaa aagtataata agacaaccac gattttatgc
    10021 gcaatttctg ttgctaattg tttagggata ccagaataac gctgatcaaa agtggaaccg
    10081 atatttttga agaaaaagac gattccaatc aaaaacggaa tgatgtagta gaacaggcga
    10141 tagagcaaga tccacacaac gactatttct cttggcacac caatagcgga caacccgata
    10201 atcatcatta catcgaaact cccaatttct cctggaatca tagacacgat tcctatcact
    10261 gaagcagcaa tgaataacgg aattgtttgc cacagatcaa tccggatttc cattaaataa
    10321 ccaacagaaa ggaaactaaa caggacaccg gtccattcca acaatgaaac tagcaatagc
    10381 tgtccttgag ttttaagtga ggtaccacca aggaatcctt tctttttgaa agtagtaaat
    10441 aaaaagacga caggaaaata tagaccgccg ccgatcaacc atatccaata ttgcttcaaa
    10501 aaaggagcaa ctggcgtgaa gactacaagt aaaaaagaaa gcagactgta aatagaaagt
    10561 ccagccatca aaaacaagaa aactttcgat aatgcttgga tcacgtttct gctctctttc
    10621 ttttgtccat atagttcaga gcgaagcccc acactaacaa acccgccgaa accggcaata
    10681 ttgttgattg tattgatcag ccagcttgtc tcaaacaagt aacgaggatt ctgcttttgt
    10741 tcaagcatcc gattcaaaat aatatcataa ccaatcatag gtaacacaga gacaagacca
    10801 atcaacagca taagtccgct tttccataca ggtatagtgg caaacgtttc tcctaattgc
    10861 tggatagata gagttttacc gatggacatc aattcaccga caataatgac aatgacagaa
    10921 atcaaaaaga ttgttttgaa cagtcctagg tggtttttca gccattgtat ggtatttttt
    10981 aacaaataaa tcaccttcta ttcctaatag ttatgtttca gaaagaaatt gaagaacttc
    11041 tcgattgaag cgttcaggat cttttctagc taattcatgt ccttgttctt tcacaagcac
    11101 aaaggaagct tttggtatag ttttagctat atacaaagaa tgttttagct tgattacatc
    11161 tttttttccc acgatgatca aagtggggga gttgattttt tttaaatcat tttctgtaag
    11221 cccgatgtca tgcagcaaaa gtttgataac taaaagattt ttgcgaagac ttgggcgaaa
    11281 taaagagagt aaccataccc atgcataatg aatattactg atgactctgg cagaaaaacg
    11341 aacgccttta acaagcgtgt ttccagaatt taaaatcaat cgatgaactt ttttagggaa
    11401 gctgctggca aataccagtg ctagatttgc tccatcacta aatccaagaa aatctgcctg
    11461 atcgattttt tccagcaaca tgatcgtatt cagatcctcg gccattaact gaaaattcaa
    11521 catagatgct tcgtttgtcg aacgaccatg tcctcggcta tctactagat acacggtata
    11581 gtagcgttcg agtacaggga cctgttctga aaaaaagcgg ccgctgccgt cattgccatg
    11641 cagtaaaaag agagggaagc cttgaccgct cttttcatag taaatttcgc tcccatctgg
    11701 catttttgca tatttctttt cttttttcac ataatcacct actgtgtaaa gtataacgat
    11761 tatatcagtc attgggcaat tgtttttact gaaaaacaac gatcattttc gtaagatgac
    11821 aaaaaataca ggaaaattca ttttttctta aggcgtacac agaaatcaag gaagaaatag
    11881 ccaagaagat aaagaaatag taagataaaa aaagatgtca aaataactag gtgatgaaat
    11941 gaagcgaaaa cgattatcta ttatccgtat tcttttttac atgatcattc tgacggttat
    12001 tggtgcggtg ttgtttttag gaggaagaac ttatcgttcg gtaaaacgag tggaaagcta
    12061 tcaaatagaa ataacaacag ctgcaaaaaa ttatcatatg gaaaactacg agaatttgat
    12121 caaggcgata atattgaccg aatcaaaagg aaaagggaca gatctcatgc ggagctcgga
    12181 aagtctgcat ggagaagctg agatcattga taatccagct gacagtattg atcaaggtgt
    12241 caaatatttg gcggaaatga tagtagaagc aaagaaacaa ggatgcgatt tatggacagc
    12301 tgtccaagca tataattttg ggaaagatta tattgcctat gtaaaaaatc atggcggaga
    12361 aaacacagta gaattagcag aagcgtattc taaagatgtg ctgagcccac ttttaggaaa
    12421 tgaggaaggg aaacaatacc gatattggcg gatccagtcc ttgctttaca atggcggcta
    12481 tctctatcat aatggaggta acatgtttta tcctgatatt gtcaaaatga atgaaaaaat
    12541 aatagagacc tatgaaaaag tttttaagta aaatagcagg aaatgttcaa tcatgaataa
    12601 aattaaatga aagaaggagt gcgcaaatag tggaaaaaag gcaaaaaatg gaaaatcaaa
    12661 aagtggggta cacaaccaaa gactggatgt tacggtttgt gaagggaatg tttatcggtt
    12721 caggatttat cttgccagga gtcagcggtg gtgcattagc agctgtcttt ggcatgtatg
    12781 agcggctgat ttctttttta gcccacctta caaaaaattt caaagaaaat gttttgtttt
    12841 ttctgcctgt tggtctaggc ggagtgacag gcatctttct actttctttt ttagtcagtt
    12901 ttttattagg tgcgtatgaa acaacgatat tatggttttt tgtcggatgt atcgctggaa
    12961 ctgttcctgc gttatggaaa gaagcaggga aaaaaggaag agatacttct gatgtgatca
    13021 tcatggttgt tacttttgtt ctggcttatt tgtttttatt atacggcgct caattatttg
    13081 acgggcaagt cgatcagaat tttggaacat ggctgattgc cggcggattg attggtttgg
    13141 gtatgatcgt cccaggattg agcccttcta attttttggt gtatatggga ttgtataaag
    13201 cgatggctga cggatttaaa gaagtacgac ttgaagtgat tgtgccgatc ggaattggtg
    13261 ggattctttg tgttcttgga ttgtcaaaaa tcatggatta cattttctca aaggcatata
    13321 gcaagctatt ccatttcatt ttaggtgtag tttttgcttc aacgattatg atcattccta
    13381 ccgattacgc gggattggga gttgctgggg tactagcttg tcctgttctt tttatagccg
    13441 gtacagcact aggttggtgg atgagccgtt tggaggaaca atataagtaa agatagattg
    13501 ataataaaaa cagcacacgg gtgattcaaa caaaaaggtt ggcatctttt ttgtaaaatc
    13561 attcgtgtgt ttttctataa attttataaa aacaagttgt ttttaatctt ggaacacgct
    13621 agacttaaac acgtgattat ttttgaaagt tggcggaaaa tggagaaaaa gaaaatgaat
    13681 ggacttacac agcaagaggt agttcagaaa atggcttctg gccagcaaaa tgactaccag
    13741 gaagatgcat caaaaagtac aaaacaaata ttcagcgata atatattgac gttattcaat
    13801 tttctgaact ttggtattgg gatttgtctg ttatttgtag gtgcgtattc caacatggcg
    13861 tatttagcaa ttattgtcgt caatatcgtt attggaattt atcaagaaat acatgcaaga
    13921 aatcttgtac ggaaattatc gattgtttcc caatcagaag tactagtgat ccgggacggt
    13981 aaagaacaat cgatttctac taaagagctg gtattaggag atttggtaaa aatcggagca
    14041 ggagaacaga taccagccga tttacaggtt atatctggac gggcagaagc aaatgaagct
    14101 cttttgacag gcgaatctga tttgattgtc aaagaaactg gagctgaatt gctttctgga
    14161 agctttttga caagcggttc tgtcatggct gaagttatcc gagtaggtgc agataattat
    14221 gctgtccgct tgactagcga ggcaaagact cacaaagcta ttcgttctga acttgtagat
    14281 tcgattcgca aagtatccaa attcactagt ttcgtgatta ttcctttggg gatcatacta
    14341 tttttggaag cactgttcat ccgcgatgct tctatcaaaa tttctgttat tgcatctgca
    14401 gcagctttgt taggcatgct tccaaagggt ttggtattgc taataagtat tgcattaacg
    14461 acaggggtaa ccaaactggc aaaaaaacga atccttgttc aagacatgta ttcaattgaa
    14521 acgctggccc atgtcgatac attatgcttg gataaaacag ggaccattac tgaaggaaaa
    14581 atgagtatcc agcgggtaga agcactacat ccgctgtacg agaaagccat cccagaaatt
    14641 atggggacgt atctggcaga aagcagtgat aacaacatca ctatgcgggc tatgagagat
    14701 tattttgagc tttctaatcg gtatgatgtg acaaacaaag tcgcattttc ttctgatcgc
    14761 aaatggggcg cgatggaatt tccagaactg ggagtcgtgt acgtaggagc gcctgaacgt
    14821 ttggtggaag aagatcggct tccggacagt ctaaaaacag cccaagcgaa tggctatcgc
    14881 gtacttatgt tagggatcgc tccaaatcag caattgaacg aagaaaaacc agcatttgtt
    14941 gagccggttg ctctgtttga aatcgatgat ccaatccgaa aaaacgctca agaaacacta
    15001 gcttatcttc atgaggaagg tgtggatctg aaagtgattt ctggagacaa tccttttact
    15061 gtctccaata tcgctagacg tgcaggatta ccgaattatg atgcgtatgt cgatctttct
    15121 caaattactg aagaaatgga agtgcgagaa gctgctcatc gctatacggt ttttggacga
    15181 gtgagtcctc agcaaaagaa attgctggta aatgaattga aagaaagcgg cagaacggtt
    15241 gcaatgacag gcgacggtgt gaatgatgtt ttggcgttaa gagaagcaga ttgtagcatt
    15301 gctatggcag aaggtgatgg agcaacacgt cagattgcga atctggtatt gttggactct
    15361 gattttacta cattgccaga agtcctgttt gaaggacgcc gagtggtcaa caatgtgacg
    15421 aaagtctcag gcatcttttt tatcaaaacg atttattcat ttattttatc gattatatgt
    15481 gccgttacag caattggatt tccgtttatc cctatccaaa tcacgctgat tgatttagcg
    15541 attgaagggt atccttcctt ctttttatct tttgaacaag ataaaagaaa aatcacttac
    15601 cgttatttgc ctacagcact tattaatgct ttaccaaatg ccttgcttgt agtagtgaat
    15661 atcattgcgg tgtatctact tggaaaaaca ttcgcatggg gaaatgctga aactactact
    15721 ttgatgtatt acttgctgat tgggatcagt tgcatggcag ttgtaaaagc ttgttatccc
    15781 ttcaacccat tgcgcatctt cctagcagta acgacgatcg tagggatata tgtagctgca
    15841 atgctgttcc atggaatttt ggaagtaaac ttagggttgg gagagagcgt aggatacttc
    15901 tttgtattca tgctcgtaaa tattgttctt cgagtattgt actggaaggc tgatattcaa
    15961 aatcagttac tgaaaagagt agcaaattga tgcttaatga gatagaaatg tgttatactt
    16021 gaaaaagcaa tgaaatatga tacaatcaaa catgtataat tgtgtccttt aaaatcgcac
    16081 tgatttcagt tagtgcgatt ttttaccggt tattctcctg aaatatcttc tgttaaaccc
    16141 aaaagatatc aatagagcag ggggtggaaa aatggctcaa cggattcctc aagaggttat
    16201 cgaaacagta cgcagtcaaa cgaatatcgt tgaggtgatt ggacaatatg tccagttgaa
    16261 aaagtctggt aaaaattact tggggctgtg tccttttcat gaagaacgta caccgtcttt
    16321 ttctgttgca gaagacaagc agatttttca ttgctttggt tgtggtaagg gcggaaacgt
    16381 gtttactttt cttcaagaac tagaaggtct aagttttcca gaagcagtgg taaaagtggc
    16441 agacttcgaa cagataccgt tagaagatca atggcgtcag cgtactatag ctgtccaaaa
    16501 tcctgaatct gctacgggga aactgattgc tgctcatgaa aaagcagcgg aaatctatca
    16561 tcacatgcta ttgcatacaa aagctggaca gccagcttta gaatatttgc aaagtcgtgg
    16621 gttgtcttta gaattaattg aagaattcaa cataggtttt gcacctaatg agcgttcttt
    16681 tcttcagcaa gtattcaaga atgaatcaat ttcaaatgaa ttgttagaac gctctggatt
    16741 gtttgtcacg catgatgatg gccgtatgtc tgatcgtttt tatcaaagag tgatgtttcc
    16801 acttcgtaat tctcaaggcc agacgatcgg tttttcagga cgctggcttg cacctgaaaa
    16861 agggcaggaa aaagatcagc ccaaatactt gaatagtcca gaaacagaac tgttcaataa
    16921 aagacaagtt ttatttaatc ttgataaagc aagaagcgat atccggaaaa acggagaaat
    16981 tttgttgttc gaagggttta tggatgtgat tgctgcatgg caggctggta tcaaaactgg
    17041 attagcttct atggggacaa gtctaacagc tcaacagatc caacaaatgg aaaagctgac
    17101 gaaagaattg gtcttttgct atgacggaga caacgcaggt tttgaagcaa ccaatcgagg
    17161 gattgaattg cttcggcaaa acagtcgctt agcactttct gtcgtagtcg ttccagaaaa
    17221 gcttgatcct gatgaatatc tccgtaagta tggagaagca tctttccaag aattagtggc
    17281 tcatgggcgc gaaacagtct ttacttttaa atcacgttat ttaaaacaag ggaaaaattt
    17341 agataatgaa aaagacaaga tcgaatacct cgaagaatta ctagttgaac ttagccgagt
    17401 tgtttcacca atcgaacaag atatgtatct atctcaactg tcaacagagt ttcagatcgg
    17461 cagagagacg atacaaaaac aactgcgcga tcttcgaagg acaaatcagc aaaatcgtca
    17521 agaacctcag gaaagtcaac acactcaggt tacgagacaa gaaactgcac ggaaaaagag
    17581 acctttgacg caagtagaaa aggcagagca gattctatta tatcgtgcat ttaaagaagt
    17641 ttctgtccgt aatattttaa aagaacgaga atttcagttt attcatgatg catatgcaga
    17701 agtttatttt ttattcgatg catatgtctc acaacattat gaattcaacc ttgcagagtt
    17761 tttagacttt ttgcaagatg aaaatttaag gcggttggtg gtggaaatcg aatatttacc
    17821 ggtggcagaa gaaagttcgc cacgagaaat ccaagacttg ctagatgtga tcagcaaatc
    17881 tggcttagct gatgaaataa cattaaaaaa acaaatgcag caagaagcca gacgaactgg
    17941 aaacaaacag cttgagcttg aattgacgat tgaaatcatc aatttaacga aacagttaaa
    18001 acaagccaaa tagctactca aggggggcct tcttttatgg caaatggaac agataaaaaa
    18061 acgtatgaag cagcagtagc agctttcatc aaagaaaaca aacccaaacg acaagttgta
    18121 tatgatgatt tatccaatca gcttgctaca ccattcactt tgaatgcaga tgaaatggac
    18181 aaattgatcc aaaagattga agacgcaggt atcagtgtgg tggatgaaaa tggtgatcca
    18241 tcaattcaca gcttgaagag tgctgagaaa aaagctgaac aagcaaaaac agaagatcta
    18301 tctgcgccaa ccggtgtaaa aatcaatgac ccggttcgta tgtatctgaa agaaattggc
    18361 cgtgtctccc ttttaactgc ggaagaagaa gtagcgttag cattgaaaat cgaagaaggc
    18421 gaccaagaag cgaaacagcg tctggcagaa gcaaaccttc gtttagtagt aagtatcgct
    18481 aaacgttatg taggacgagg catgcagttc cttgacttga tccaagaagg aaacatggga
    18541 ctgatgaagg cagttgaaaa atttgactac cgcaaaggat ttaagttttc aacctatgct
    18601 acatggtgga ttcgtcaagc catcactcga gcaatcgctg accaagcacg tacgatccgt
    18661 attccagttc atatggttga aacaattaat aaattgatac gtatccaacg tcaattgctg
    18721 caagaccttg gaagagaacc tacgccagaa gaaatcggtg cggaaatgga tcttccaaca
    18781 gaaaaagtcc gagaaatctt gaaaatcgcg caagaacctg tttcacttga aacgccaatc
    18841 ggtgaagaag atgactctca cttaggtgat ttcatagaag atcaggaagc aacaagtcca
    18901 gcagaacacg cagcttatga gttactaaaa gaacaattgg aagatgtact agatacattg
    18961 actgaccggg aggaaaatgt ccttcgctta cgttttggtt tagatgatgg ccggacgaga
    19021 acattggaag aagtagggaa agtatttggt gttactcggg aacgtattcg tcagatcgag
    19081 gctaaagcat taagaaaatt gcgccatcct tctcgttcaa aacaattaaa agatttctta
    19141 gaataagcat gaactattga ccagaagaga aatcagtaaa actgtttggt ttattttaaa
    19201 agaaaaaaag cttgagacta cgcagtctca agcttttttt tctataggaa ggaaattcat
    19261 tcattatttt taagtaaatc ctaaaaataa gctattggcg tgacgtatag aatatgatag
    19321 attaaatggt agcgtaaacc tagtaaagga gccgatcgct aagtgatgaa aaaatgtcca
    19381 aattgcggat cgaaaaaaat ggagcagaat actatctgtc cagactgcgg tcatcaaata
    19441 actgcagaag atcagcaaat aaaagaatca attgaagaaa caaatacaga tgcagctatg
    19501 caggataaat caactgaaga tgcagtcttt cctgatacag aattaaacga tccaatcgaa
    19561 tggtcggaat tgaaagattt acctctagaa tctgtaatgg aattattcga aaaagcagaa
    19621 ccagaagacg aagaaccacc tgctcacgat caagtaacta atatagagaa aaatgaacag
    19681 aagaaagcaa ctgttcatga gacttcggaa gaatcaggaa agaatcaaga agaaacgctt
    19741 gaatcaaaac aaaaaagaag agtagctgaa ctgaaagaaa tcgtcgataa tgaggaagaa
    19801 aattcgatac tatccgctta tatcaaagca catcgtgaag atacaaaaga agagcacgcg
    19861 aaagagctgc tcaaaatgat cagtgaaaaa attgcacagg aaaacaaagt agagccagaa
    19921 catgaagctg aaaaagacac tgaaaaagac attagtggag taaacgctgc aggtaaagaa
    19981 tcattaaaaa cgaatgatct aaaaacgaat gatctaaaaa cggatatccc ggaaaaaact
    20041 gaaacattgt ctgaaaaaca atcagaagac gagaaatcga atgggttgcc ggaagcagat
    20101 aaaaaagaaa tgctcgaaaa agcagacaag aaggcaacag agtcggatgg aataaataat
    20161 gaagccgatg ctgaaactca atctgcaagt gaagatagca ctgctcttga agctaaaata
    20221 gaaaaagaag agaaccagcc tgagctgatt gaagcagaaa cagaaccaat aacaatcgtg
    20281 ccaaatactg aaaaaacgcc tcaagaatca aacccagaaa aaaaagattt agataagaca
    20341 caaaagaaaa aatcaaaaaa agtgccgtat ctcgctttag cagcagttct tctactaggt
    20401 gggggaggct gggcttatta tgaccatcat caaaaagtag aagccgaagt agcagcagaa
    20461 gcaaaaagac agcaacaaat ggaagaactt catgagaaac ttgctgcctt ttatacagat
    20521 gatcaccatc aatttattcg tgcagatatg attcatcaag atttatcaaa actaaaaaat
    20581 gatttatcca gtcttaagaa tgaaaaagga tattcagagt tagaaacaac ttaccaagat
    20641 atccaatcta agatccgaga tatccaaaaa gtgaatgagt ggttcatagc acctgccatc
    20701 gtcgatgatc atctagcaga tgacatcaag ttgaaagcag ataaagagat ccaagcaatc
    20761 gaaacggata acacagattt tggaaaattg attcaacaag cacaaacaga agctaaaaat
    20821 caatatgctg aattgcaaac agctaaagaa aaaacagcta tagtctttaa agacggaaaa
    20881 gtagtggatt cagcaacacg cgaccaatac aagacggcaa aagaagcagt agaaaaagtg
    20941 aaaaatacag atctatctgc tcagttgaat gaacaattgc aacaagtaga taagatgctc
    21001 actgataaag agaaaaaaga agaagaggaa aagaaaaaag cagaagaagc gaaaaaagca
    21061 gaaaaagcga aaaaagcagc tgaagaacaa gcaaaacaag ccgctcaagc agcagctgca
    21121 gctcaagccg ctcaacaagc tgcggtcgca caagcagcac agcaagaagc ggcatcctcg
    21181 accccttcct cttcaaactc agcgaatcag ccaatcaaat ctgccagagc aagtgatatt
    21241 gccgatgcat ctaatccagc atggacgtgg gcaccaggtg tccaaagcaa agtgattgct
    21301 acatgtatcc aacgtggata tatcgttgaa ggaggataca gattagaaaa agccagaatc
    21361 gaaaatgggg aaggctatta caatctatat gccacttcaa caaaatctgc attgatgaaa
    21421 ggcattggag aaagtgcttt gccattttac atcgtaacca tcaactgtaa aactggatgg
    21481 ttcggaggaa atgggagcaa ttaattttag gcaaatgaaa gaggctgtga gcctgccgtt
    21541 taacaccgtt tattcgaaaa aggggagtat gacacaactg ttgtcatact ccttttttca
    21601 cctttccaaa ccaaggctca gctattcaag tagataacat tatgttatga tagcaaagaa
    21661 atgtattaag aaaacctttc actatagagg gagaaaaaaa tgaacgattt attagcaact
    21721 atatttacag gtattattat tgatgaaaat gaaaaaaact actttgtcca aaaaaacgga
    21781 caaacctttg gcttaagcaa atcagaaggt gaacacaaaa tcggtgaagc agtggaagga
    21841 ttcggctacc aaaatcaaaa acatgaaaat cggatcacta cacaaattcc taaaagccgt
    21901 atcggtcatt acgcttttgg aatcgttact gctacacgaa aagatttagg tgcgtttgtc
    21961 gatatcggtc ttccagataa agactttgtc gtatctcttg atgaattgcc gacaatgaca
    22021 gagctatggc ctaaaaaagg ggatcgcttg atgatttccc ttagagttga tgcaaaagac
    22081 cgtatttggg gcagtttagc agatgaaaaa atattcaaat ctttaagcaa atatggaaca
    22141 gaagaaatga aaaacaaaga tgtgatagga acagcatacc gcttgaaatt aacaggtacg
    22201 tacgttttaa cggaagattt ctatcttggg tttatccatc cttctgaacg atataatgaa
    22261 cctcgtctag gcgagcaaat aaaaggtcga gtgattggtg tccgaccaga cggtgtactg
    22321 aatctttcat tgaagccaag agcatatgaa gctatatcag atgatgcaca aatgatttta
    22381 accattttag aacgttctgc agatcagcaa atcaacttta cagataaatc ggatccagaa
    22441 gaaatcatga aagcttttgg tatcagcaaa ggagcattta agcgcgcatt aggtaatctt
    22501 ttgaaacaag gattgattac acaagaaaac ggtgtgacaa aactagtcaa aaaatctaat
    22561 tgagaatccc ttgcatgttt ttcttaacta gcttataata attttaatta acatagtgtt
    22621 atatcttttt ttatttgtaa ttattataaa ttgttggggt tgagtaaatg gtcatgaatg
    22681 ctatttcggc gatgaaaaaa acaaaacagc agctacatga agcggggttt aagctgacac
    22741 cgcaaagaga agctactctg cttgtgcttt tggaaaatga gaaagagcat ttatctgcag
    22801 aagaaatctt ctttttagtg aaaaaaaaga gtccggaaat tggacttgct actgtttatc
    22861 gaacccttga aatcttgaca gaattgaaaa ttgtcgataa agtaagtttt aatgatggat
    22921 tagcccgtta tgatttgaga aaagaaggcg ccaaacattt ccatcatcat ttactatgct
    22981 tgaactgcgg cacgatcgag gaagtagaag aggatctttt acttgaagtg gagcaagtag
    23041 tagaagaacg ttatcatttt ttagtaaaag atcatcggct aacgttccat ggaatctgtc
    23101 aggaatgcta tgaaaaagaa aaaagaaacc agtccgtctg agtgtctaga cggctttttc
    23161 tttgctcaga ttatggaaaa agactatcag aatgaagcgg tatctgatat gatagagagg
    23221 catgaggggg attattatga aagagcaaat caacgattat ctccactact tgaatattga
    23281 acggggattg tccgccaata cacgacaaag ctatgaacgt gatttggaac agtacttgca
    23341 ttttctacag gagcagaaaa ttgcttcatg gcaggatgtc gatcgctttt tagtcatccg
    23401 ttttctggag aagttgcatg aagaaaaaaa agcgacagca actgttactc ggatgatctc
    23461 aagtctcaga agattccatc agtttttacg gcaagagcgt atcactgatc atgatccgat
    23521 gcaacatatc gatacgccga agaaagcaca gaaattacca agtacattga ctctgacaga
    23581 agtagaaaag ctgattgaag cacctgatac gacaaaaacg ttagggataa gagatcgtgc
    23641 aatcttggaa gtcatgtatg ctacagggat gcgcgtcagt gaattgattg gattgaaatt
    23701 gggagatctt catctttctc ttggactggt acagacagtt ggaaaaggag ataaagagcg
    23761 gatcattcct ttaggtgatt atgcgatcca atggctggaa cgctacttag acgaagcacg
    23821 tccattactt gtaaaggacg catctgaaat gcatgtattt gtcaataatc atgggaaagg
    23881 tctttctcgg caaggaatct ggaaaaatct gaagcagcta gtacgagagg ctgggattta
    23941 caaagaagta acaccacata cgctgcggca tagctttgcc acacatctgc tagaaaatgg
    24001 agctgatttg cggacagtac aggaattgtt aggtcacgcg gatatctcta cgactcagat
    24061 ttatacacat atcacgaaga aaagaatgac ggatgtttat aagcagcatt ttccaagggc
    24121 ttagtcatag gaaggtgaga agatgttaac acattaccgg aaagaaaacc gtaaaattgc
    24181 tatgggacta ttgagtttcc ataagaaact gacaggtaaa acttctttgt tgaaggagat
    24241 tgatacgtac gaatctgaag acgattatga actttttttc tttacgccag aaggttcaac
    24301 caacattcaa gggattattg gtgtttactg ggcaacgaac gatcagctga tcatccatga
    24361 catttctttg aatccgtctt atcgcggtga gaatatgggc tttcaaatgt tagatgaatt
    24421 acaggaaaag tacccggatg tctccatccg tgcaactgaa atcacagagc cttacattgg
    24481 aaaatggcta gcaaagaaaa agaagaaaga gtgaatgagt tgacggagat caatattaaa
    24541 ttagatgtat tcgaaggtcc tctcgatctg ctgcttcatc tgattcagca aatggaaatc
    24601 gatatatatg atataccgat cgctacgatc acggagcaat atatgaatta tatccataca
    24661 atgaaaacac tagagctagc tgttgcagga gaatacttag tgatggctgc tacattgatg
    24721 gcaatcaaaa gcaagacatt gcttcctgtc caagaattcg tcacagatga tgaagaatta
    24781 tgggaagaag atcctcgaga agaattggtc actcagcttt tagaataccg taaatataaa
    24841 tatgcagctg aaagactgac agaaaaagca gaggaacgaa gtctttatta cacaaaagaa
    24901 cctatgaaca tcgatgggtt agcggatgct gaaaagccgt tgaagcgagg acaagtcaaa
    24961 aaaatcgatt tgatccgtgc catgcagaaa atcttagaac gtaagaaatt ttctcaaaaa
    25021 gtggaaaaaa cgatcacgcc cgatgacaca acgatcgaag aacgaatggt atttatcgaa
    25081 gagcgtataa aaagaaatgc gcatggctgt actttcgatt ctttttttga gtcaccttct
    25141 aaaagtgaag ttgtcactac ttttatggct ttgctagaat tgatgaaaaa agggcagatc
    25201 attgcgcaac aaaaagaaaa ctatgaatca attatgcttt atccagcagt aggagatacc
    25261 gaacatgacg aaattaagtg aacttgaagc ccttcttttt attgttggcg aggaagggat
    25321 cagtaatgaa gaattaagcg gactgttaaa tctcagcgaa gcagaagtca caagtttagt
    25381 gaaagaatta aaagaatctt acactcaaaa ctcgcactca gctttgcata tttttgaaac
    25441 ggaaagccat tatgtattaa cgactaaaaa agagctggct cccttactta agcgatatgc
    25501 gcaagggaca tcaaacacat tatctcaagc cgctgtcgaa accttagcaa tcatcgctta
    25561 taaacagcca atcacaagag ctgaaatcga gcagattcga ggagttcagg tttcaggagc
    25621 ggtacagcgt ctagctgccc ataaactgat tgaagaaaaa ggtagagtag aagggccagg
    25681 tcggccgata ttatatggaa caactggcta cttcttagat tattttggtt tgaaatcatt
    25741 agaagaactg cctgatacac aagaaatgga aatttcattt gaagaagacg tgtcaccaga
    25801 tctattcttt gatggaatga ataaaggaga ataattgtaa tggaaagatt gcagaaagta
    25861 attgcccacg ctggcatcac ttctcgtcga aaagcagaag aattcatcct tgcaggacgt
    25921 gtaaaagtaa acggacaagt tgtaagggaa ttagggataa aagttgggaa acatgacact
    25981 gtggaagtgg atgaagtccc aatttatcaa gaagaatacg gttattattt attgtacaaa
    26041 cctaaaggag ttatttctgc tgttgcagat gacaaaggaa gaaaagttgt tacggatctt
    26101 ttgccgatgg taaaagaacg tatctatcct gtaggtcgac tggattacga tacttcgggg
    26161 atcttgctac tgacaaatga tggagatttt gctcagcgcc tgacacatcc aaaacatgaa
    26221 gtggataaag tttacgttgc aaaggtaaaa gggatcgcta caaaatcgat gcttgcacct
    26281 ttgacaaaag gaataaagat cgaaggacga aaaacatctc cagctaaatt ccaaatttta
    26341 tcagtggatc ctaagacaaa ccacagtatc gtggaattga cgatccacga aggacgtaat
    26401 catcaagtga aaaatatgct tcaagcagtt ggtcttcctg tgcaaaaact aaaacgtgaa
    26461 aaatacggag atctgacttt acaaggccta cgtcctggag attaccgtga actgaacaaa
    26521 aaagaagtca gcagtttgct gaaccactca aaatgaacac ttacttttta taagtgttag
    26581 atgttgcgtt ttttgaatag atatgtataa tgaatatgta aacaaaaatt atataaggtt
    26641 cgtcttcagg ggcagggtga aattcccgac cggtggttat agtccacgac ctattttgca
    26701 aaaaatagct gaatcggtga aattccgata ccgacagtat agtctggata aagaagatag
    26761 agcttatttg acgacgcttg atcaaataac tctaactcta ttttccctgg aaaatagagt
    26821 tttttcattt ggagagaaac gtcaaaaagt tcgctgaaga ggagtcctaa caggaggatt
    26881 tcaaatgaag aacacccgtg tacagaaaat ggttgcagta gcgatgtttg ctgcgatcgc
    26941 gttagtttta cagtattttg cttttccgat catgccggct tttagttttt tgaagatcga
    27001 ttttagtgat atcccggtat tgatcagcat gttcttattc ggtccagcgg ccggagttgc
    27061 aacagcgttc ttgcgttcag tattacatct gctgactaca ggtttttctc cggataattt
    27121 ggtaggagac actgctagtt tcttatcaac aacgatcttt acattcccga tttattattt
    27181 ctttagtgta aagaaacagc ataaaggaag aaacaaattt ttaggtgtca ttacaggaac
    27241 catcgctcta actattttta tgagtatcgc aaattatttc gttattacgc ctctttactt
    27301 aatgttttta ggactgaacg caacacagat gctaggcatg tctttagcaa actatgtact
    27361 gattgggatc gttcctttca acttgatcaa aggattcatc gtgagcgctg tctttttagt
    27421 gctgcatgcg caacttcttc catggttaag tcgaaaacaa catcagttcg aacaacgtca
    27481 tacagcagtc aaataattat caaaaagatc aagaaatttc ttggtctttt tttttggaaa
    27541 aaaatacaag acatgcaata atgtaaacgt tttatttgtt tgttttgagt atttttttga
    27601 tttatgcata aaaatcaatc aattctttgc aaatgtgaaa ttcttctcct tttaaaagac
    27661 gaaaatgttt gtttataaaa aaaaggaaac gctacaatga aaaagaagaa agctttaaaa
    27721 acctgaaaaa agaggaggga atagggaaaa acgaactttt tgaacaaaag ttttacaaaa
    27781 tgcgtgttat tatttttgtt ctgatactct ttttgataaa ataacattag agccaaaatt
    27841 gttgtctcta acggaaaaga aaggaagaag aagatggaac aaacaaaaaa gaaaaaattt
    27901 caaatgcctt cagcgtatac ggtcttattt attattattg cagtaatcgc tgtacttact
    27961 tggtttatac cagctgggca ctatagtgta gacgatgcag gcaacatcgt cgctggatca
    28021 tatgaacgag tacaacaaaa tcctcaaggg ctgtgggata ttttcatggc gccaatcaat
    28081 gggatgttag gtgtaccagc gggggagcta actaaagaaa cacctgctgc aatcccaatc
    28141 tctttcttca ttttggttgt tggtggattt ttaggtatta tcaacaaaac aggggcacta
    28201 gatgccggga ttgcttcagt tgtgaagaaa ttcaaaggaa aagaaaaaat gcttattcca
    28261 gtattgatgc tgttatttgc attaggcggc tctacttacg gaatggcaga agaaacgatt
    28321 ccattctatg ctttactgat cccagttatg atggctgtag gctttgatac agtagtagca
    28381 gtagcaatcg tattagtagg gtctcaagta ggctgtctag cttcaacggt aaatcctttt
    28441 gcaacaggcg tagcttcaca agctgttggg atttcgatgg gtgaaggtat tggtcttcga
    28501 ttcttgatgt tcgttatttt gttagctatt tctattgtat atgtgtatcg ttatgcatca
    28561 aagatcgaaa aagatccaac aaaatcatta gtatacaacc aacgtaaaga agatatgaag
    28621 catttcgata ttgaagcaat cggtcgtcaa gaagtaatca cgaaaaaaca aaaacatgtt
    28681 aatgtattgt tctttattac ttttggtatc atgatcatca gtttgattcc ttggacaacc
    28741 ttgaatgcga atttcacgat ttttgaatca ttgacaaact ggttaacaaa ccttccggta
    28801 ctaggtactg ttctaggtaa aagcatgctt ccattaggtg actggtactt cccagaaatt
    28861 actatgttat tcttagtaat gtccatcgta gtagcaatca cttatggcat gaaagagtct
    28921 gaatttgtct ctgaattttt agctggtgct tctgatttga tcggtgtagc aatcgtagta
    28981 gctgttgctc gtggtatcca agtggttatg aacaatggat taatcacaga caccatctta
    29041 cattggggcg aacaaggatt aagttcatta tcttcaagtg tattcatcat tattacattt
    29101 attttctata tccctatgtc attcttgatt ccttcaactt ccggcttagc tgctgcaaca
    29161 atgggaatca tgggacctat gggtgaattt gccggcgtcg gtaaggattt agtaatcact
    29221 gcttatcaat ctgcatctgg tttagtaaac ttaattacac caacatctgc tatcgtaatg
    29281 ggtgctgtag caattgctcg tttcgatgtt tcagtgtggt ggaaatttac cggtaaatta
    29341 attgcgatat tatttgtagc catctgtgtg atcctaggtg tagcagcaat gttctaaacc
    29401 tagacaaata caggtacaag gtctatcata agaatgaaga aaccgataaa cggctatcga
    29461 taagcacgtg gacaagtcaa tagctccatt cagtgagtgt tttttctttc atcatttgtt
    29521 aagaacattc tgcttgctga tggagcaggc ttaaaacgtc attatttgag aagaagaggt
    29581 tgtgtcggag gaatttgaca tgaccctttt taacatatta ggaggataat atcatgaaaa
    29641 aatttgtgac agaacaacat catgaacaag cattagaatc attgagcgaa ttgatccgaa
    29701 ttccttccgt actagatgaa gcagattcag gacaaggaca tcccttcggt aaaaaagtga
    29761 ttgaagcttt ggataaagta ttggaaatca gtgaaaagct aggatttaga acatttaaag
    29821 atccagaagg ctattacggt tattcagaga ttggctctgg agatgaatta ttcggtatct
    29881 tgtgtcatat ggacgttgtt ccagcagggg acgaaaataa ttgggaaaca aaaccattcg
    29941 atccaactat aaaagacggc tggttagttg gtcgcggatc ccaagatgac aaaggtcctt
    30001 ctattgcggc aatgtatgcc gtcaaagctt tgattgatgc tggcgtggaa ttcaagacac
    30061 gtattcgttt tatctttggt acagatgaag aaaatttatg gcgttgttta gaaaaatata
    30121 acgaaaaaga agaaggaatt acgcaaggat ttgcgccaga tgctgaattt cctttaattt
    30181 atgcagaaaa aggcttattg caagcttact tgacaggacc aggaacgaac gagttttctg
    30241 taaaagctgg aggagcgttg aatgttgttc ctgatgcagc tccttactct ggagaaaagc
    30301 tttctgaagt aaaagaagcg ttgaaaaaac acgaattcga tttcgaagaa caaggtgaag
    30361 gcatcgtcgt tcttggtaaa agtatccacg cgaaagatgc agcacaagga gtgaatgcta
    30421 tttctcgtct ggcaattgct ctttcagaag tattcgattt tggcccaatc aatttcttag
    30481 gaaaattagt tcaagaaaat gcaactggtg aagctgtcgt gggcaaaaca gaagatgaac
    30541 aatccggcga gctaacgatg aactttgcaa gtttggaaat cacaccagaa caaacaaaaa
    30601 tcggtgtaga tatgagaatc cctgttacat tcaagaaaga tgatttagta gctaaattaa
    30661 ctaaaacagc agaaaaatat gggttgactt atgaagaatt tgacttcctt gattcattat
    30721 acgtgccact agacagtgag ttagtgaaaa atctattggg gacatatcgc gatattactg
    30781 gagacatgac tgaaccgttt gtatcaggcg gcgctacttt tgctcgtacg atgaaccagt
    30841 gtgtggcatt cggtgcgatg ttccctgata ctcctgactt tatgcaccag gcgaatgaac
    30901 gatgggaatt aagtagtatg tataaagcaa tggaaattta tgcagaagct gtgtatcgtt
    30961 tatgtgctaa ataaaaataa acatacaaaa agggcggata ttcaaatgga tattccgtct
    31021 ttttttattt gatgctggaa agtttgcatt tctctcttat tttttttaag gtataatcat
    31081 acttgattaa gaaaaagggg tcactcagat agaaaaggtg ggagaaagtg aaaatattta
    31141 gacgaatact aattggctta gtcatcattt taggattact cggtggagcc ggttattact
    31201 atatacagca aaatacgtac gaaccatcgt cagaagcgca agtgttggta aaagaaggaa
    31261 aaaacaaaga agattactta ttttttgaag gagagaaaaa gaatcatgga aattctccat
    31321 tacttatttt ttatcctggt gctctcgtag agccagaaag ttatagtatt tgggcgcaag
    31381 atttagcaaa aaacggcttt tcagtcgcta ttgtcaaaat gccacttgat ctagcagtga
    31441 tgggcggaaa caaagcggat aaggtcaaag aagattttcc aaattccgat tatgtgatcg
    31501 gtggccattc attaggtggc gtaatggcaa gtcgttatgt caataagaat cattcagaca
    31561 aacatttaaa aggggtattc ttcatggcaa gttatcccga caaaaaagga acattgacag
    31621 aatcagctgt tcctgtttta tcgatcacgg cgacaaaaga taaagtattg aatcaggaag
    31681 cttatcaaga agctaagcag tatttaccgg aaagaacaga gtatgtatca atcaatggcg
    31741 gaaatcatgg aggttttggc agctatggtg agcaaaaagg agatggagag gcagaaattt
    31801 ctaacgcaga ccagcaaact gtgatcgtca atacattgga aaactggctg aaaaagttga
    31861 aataatataa atgggtccta tagggtccgt gaaaaaacta gtcaattaac tatagatagc
    31921 aaatgaatct ttcaaaataa gtcgggattt cacggaacaa gagaaacaga tcccggctta
    31981 ttttcaagtt tagagaactt agtgtacgtt tttaaagttt cgtttatatt tatattttat
    32041 tttctaataa aatcgctcga actggacatt tttgatacgc tttttttaca tatgtttctt
    32101 cttccggcat cacgaactgt tgatgagcct ctggttcttg ggcaaataaa acgattcctt
    32161 cttcatcata atcgaaaata tctggcgctt cgatttgaca aagaccgcat gcaatacagc
    32221 gttcaggtac aagtttacat agtagtgaca tttctaacca tcctaactta aggagaataa
    32281 tatgattgtt tttattttat cgctattttc atcaggacac aagctgcgga ttagttctct
    32341 ttaccaactc cttgtgggaa aacgaacgac ttcagtactt atttatggat ttacccacga
    32401 acttttgttt atccataaca gttttccaga attgaagcag gataagtttt atcaaatttt
    32461 gcaaaaaatt gcgcagcaag gttggatcga aataaatgaa aatgaagcaa agctaacgcc
    32521 tgcaggggct gatatgttgt ccgaacatca aatagaacat actggtttgc gttttgaccg
    32581 ctatggcaga acaggggaaa ctagttggcg gctcattaaa tttgctgttc aagtaatttc
    32641 taatctggca acgggtattc aagactatct tccagcagag acaagtcctt tttatacttt
    32701 ccaactaaaa aaatggctta gtgaaagcca attgccacgt gaaattttga ttgatactgc
    32761 gtatgagagt ttggtgcagc tgttttcgga gataccagaa gaagcagcag attttttagc
    32821 taaccaatta tctggaaatg agcgaacagg gctgcttcct tatcagctgg caaaaaacaa
    32881 tgatgagtca gcggtttatc tgcaacaaag cagatgtatc caccttttgt tagcacaaat
    32941 cgaaaaacgg ccagattcgc tttggcacgc tttgatcgat tcattattac agcaaaattt
    33001 caatcaaagc atgatgatca ccaagcagat gtttatgaat ggtcagacaa ttgaccagat
    33061 tatggcaatc cgtcatttaa aaagaggaac agtaacagat catcttattg agtgggcctt
    33121 gttttttgat gattttccat acgagcggat tctctctgca gaaacaatcg aacgtttgga
    33181 accaaataag gattccgtgc gagagtggcg ctttagcgaa tggaatgtag atggacaatt
    33241 agattatgga gaatttcgtt tgtatcaaat ctatctattg agaaaggaag cgatacagaa
    33301 tgttaataaa tgaattaaaa aaatattttg gatttgattc atttcgtccc ggccaagaag
    33361 aaatcattca ggcgttgctt gatggagaag atactctcgc tattttgcct acaggaacgg
    33421 gaaaaagcct ttgctatcaa ttgacggggt ttttaatgga aggtctggtc atcatcgtgt
    33481 caccgctgct ttctctaatg gaagatcaag tgacgcaatt acaaaaacga ggggagaaac
    33541 gagtagctgc tttcaatagt ttactttcgc aatctgaaag aagatacgtc ctccgtcatc
    33601 tatcgcaata taaatttttg tttttgagtc ccgaaatgtt gactaatcct tctcttttag
    33661 agcatctgaa aaaacaaaat attgcgttat atgtagtaga tgaggctcat tgcgtgtctc
    33721 aatggggagt tgatttccgt ccagaatatc aacagttagg aaaaatccga aagcttttag
    33781 gaaacccggt cacactggca ctaacggcga cagcaacaga tttagtagca agagatatac
    33841 gtcatgtact atttgatcga ccaccaaaag agatcagaca atcagtcaat cggcaaaata
    33901 tctcgctttt tgttcggaaa acgcagcaaa aagaacaaga acttgaacac ttcatggaaa
    33961 gagcacaagg agcggcaatc atttattgtg caacaaaaaa agaagtcgaa cgattgtatc
    34021 atttattcag ggaacgtttt acagttggat actatcacgg tggtcttgat gcagctcaaa
    34081 gaagacaact tcaacagcag tttgtaaaaa atcagctgca gtttctcatt gctacgaatg
    34141 catttggtat ggggatcgat aaatctgata tccgttatgt gatccattat gatttgccag
    34201 acagtctaga aaactatgta caagaaatcg gtcgagcagg cagagatcag gaagaaagtg
    34261 cagcaattct tttttatcaa agtggggatg aacgtatcca ttatttcttt aatcaacttt
    34321 ctagagaaca gcgacaaagt tttgagcttt atttggaata tgccacagag caagctccct
    34381 ttgatgagct gcaaaaaaaa tggatggagc tgatccagca gtctgaaaaa ccagaaaatt
    34441 gggtggaacg gctcaaacat caggaaaaag aaaaagaatt ccgcctgcag cagatgcttc
    34501 gctatattaa tgaagaaaac tgccgcagaa aattcatctt agcttatttt ggcgaaaaac
    34561 tctccgaaaa gccgcagaat tgttgtgata ttgatggagc aaaagcagaa cttctgccaa
    34621 agaggaagct tttttctaag actgaacaac ttcattggga aacgatttta ctaaatttat
    34681 ttaaaaaaaa catggaacat tgatttttta ggacaagaca ctttttccta tggtataata
    34741 cacacgatag agtttaggag gtaaaacagt gagcagaaaa gatagacatc aatctacaga
    34801 aaataacgaa acacaagaac catgggaaca accaatctac gatacagatg atgaagtttc
    34861 atcaagaagt tctcagagac aacaaaagaa aggaaataca ttatttcttt cgctattttt
    34921 gattttactg gtcttatgta tcgctattcc agcaggtgct tatttctgga tcagaaatgg
    34981 atcaagcaac aatgcaacag cagcaacttc ttctgaaact tcgacatcag tagtagaaag
    35041 ttctacaagt acaacaaaag aggagtcaac tgagacttca acagtagaat ctagtacaat
    35101 cgcggaagaa tctactgtgt ctcaagatac aggtgtaacg tcagaaagca gtattgcagc
    35161 agagacaaca ccgacttcct caagtgaggc aacttatcaa gcaggggatt caagcagcaa
    35221 tgcagcagaa acaacacctt cttcatcagc tgcagccggt tcaacaacga ctgttcaagc
    35281 aggcgaaggt ccaaaacaag tggctgagcg tgcagggatc accactgatc aattgtttga
    35341 attgaatggc ttagatccaa acaactttat gctttaccct ggtcaagaat taagaattaa
    35401 ataataatag ccgcgctttg cggctttttt tttgagaaaa aggagttata tcatgacaca
    35461 aatcagtatt gcaatcgacg ggcctgcttc atctgggaaa agtaccgttg ccaagatatt
    35521 agcaaaagaa ttgaattata tttatacaga taccggtgcc atgtatcgcg cagtaactta
    35581 tttagctatt acgaacaatg tatccttttc agatgaagaa gggctagttt cgctgatcaa
    35641 aaagtatcct atctcttttt cgcaacaaga agatggacag catgtattca tagcatcaaa
    35701 agatgtgact ttagaaatcc gtcaaccaga tgtcacaaaa gctgtttctg aagtctctgc
    35761 acatggaaaa gtcagagaag aattagttgc tatccaacga aaaatagggg aacgcggtgg
    35821 cgttgtcatg gacggaagag acatcgggac agctgtatta ccgaatgcag aagtgaaaat
    35881 ctttttagta gcaagcgtaa gtgagcgtgc agaaagacgt ttcaaagaga accaagaaaa
    35941 aggaattccg actgatcttg aaacactgac gaaagagata caggaaagag accattatga
    36001 ttcaacaagg gacgtctctc cgctgaagca agcagaggat gctgttcgta tcgatacgac
    36061 tggcaaatcg attccagaag tcgttgcagc aataaaagct gttgttttga aaaaaggtta
    36121 tcttcttttt taaattaaga aaaaaaatga aaaacaattg ttttttcaag gaaaacgaac
    36181 gaaatcgctt tatttttttt gaataatcaa ttaaactaaa aacagtaccg tacttacggc
    36241 agaatgttgg ttaggaggac aagtgttatg acagaatttg atcaaaatca agagaacaac
    36301 cagtcaatgg aagacgcaat gaaaagcgta caagaagtaa aagttggcga tgttgttaag
    36361 ggcgaagttt tagtcattga agacaaacaa gtgattgtcg gaatcgaagg ggctggagtt
    36421 gaaggagtcg ttccggcaaa agaattatca actttgccgg tagaagatat cagcgaagta
    36481 gtaaaagtcg gtgatgtatt agacttagtc gttatcagta cgatcggcaa agataaagaa
    36541 aacggcagct acctactttc aaaacgccgt ttagatgcta aaaaagtttg ggaagatatc
    36601 gaaagagatt tccaagaggg caaggtgatc gaagcacctg ttacaaatgt tgtcaaaggc
    36661 ggtttagtcg ttgacgtcgg tgtacgtggt tttgtccctg catcaatggt ggaagatcat
    36721 tttgttgcag acttttcaga ctataaaggc aaaacattga gctttaaaat catcgaaatc
    36781 gaaccatcag aaaatcgttt gatcttatcg cataaagcgg tagtggaaaa cgaaagagaa
    36841 gaaaagaaaa aagaaattct ttcaactatc catgagggag atgttctcag tggacgtgtt
    36901 gcacgtctta ctgattttgg cgcatttgtc gacctaggcg gaattgacgg tctagtccat
    36961 gtctcagaga tttcacatag tcatgtagca aaaccaagtg atgtattggc tgtcaatgat
    37021 gatgtaaatg tcaaagtgtt gtctatcaat cctgaacaag aacgtgtttc cttgtccatc
    37081 aaagacacac agcctggacc ttggtcagaa atcgatacaa aagctccagt tggcacagta
    37141 ttagacggaa cagttaaacg tttgacaagc ttcggtgcct ttgttgaagt gttccctgga
    37201 gtagaagggc tagtccatat ctctcaaatt tcacataaac atattgcgac accacatgaa
    37261 gtgcttcatg aaggtgacga agtgaaagtg aaagtattag agatccatcc agaagaacat
    37321 cgtattgcat tgagtatcaa agctttagaa gaaaaaccag cttcagaaga agaaccaaaa
    37381 aatgttgagt cttatgaatt accagaagaa aatactggat ttactatggg agacatttta
    37441 ggtgatgcgt tgaaagaaga agtagaagaa aaagcaaccg atgattctga aaaataattg
    37501 attttgtatc tgtgactgag atgaaagcag ctgcttttgt ctcagttttt cttttgaaaa
    37561 gcgattagta tttcaataag gaataaaaat acttcgttta attgacaatc tatgcttgaa
    37621 agttggcaat gcattcgtta aactaagtga gattggaaac ttgtgaggag gaaataatat
    37681 ggcaaatcca acaattgcaa tcgttggccg cccgaacgtc ggtaagtcta cgatttttaa
    37741 ccgcatcgct ggtgagcgta tctcgattgt cgaagatact cctggagtaa ctcgtgatcg
    37801 tatttatgca aaaggtgaat ggttaggtcg agaattcagc gtgatcgaca caggcggaat
    37861 tgatcttggc gatgaaccct ttatggatca gatcaagcat caagcagaaa tcgcgattga
    37921 agaagcagat gtaatcattt gtgtagtcag cggacgcgaa ggcgtgacag acgcagatga
    37981 gatggttgcg aaaattttat acagaagtaa taaaccagtg attttagcag tcaataaagt
    38041 agataatcca gaaatgagaa acgatatcta tgaattttat tccttagggc taggcgatcc
    38101 ttatcctgtt tcaggaagcc atggactagg gattggtgat attttagatg aagcagtgaa
    38161 gtatttctct gaagagactg aagaagagga cgacgacacg attaagttta gtttgattgg
    38221 tcgcccaaat gttggtaaat cctctttgat caatgcgatt ttaggagaag aacgtgtaat
    38281 tgtttcggat attgaaggaa cgacccgaga cgcaatcgac acccacttcg tttctgaaag
    38341 tggtcagaaa ttcttaatga tcgatacagc tggtatgcgt aaacgcggaa aagtatacga
    38401 aaatactgaa aaatacagcg tgatgcgtgc aatgcgtgcc attgaccgtt ctgatatcgt
    38461 tttgatggta ctgaatgctg aagaagggat tcgggaacaa gacaagcgag tagcgggtta
    38521 cgcccatgaa gctggtcgtg gaattattat cgtcgtgaat aaatgggatc ttgtaaagaa
    38581 agaaaccaat acaatgcgtg atttcgaaca agagattcgt gatgaattcc ggtatcttga
    38641 ttacgctcca atcgtttttg tttctgcagt gactaaacaa cggttggaac gtctgcctga
    38701 aatgatcgaa caagtaagta tgaaccagaa tcttcgtatc tcttcggctg tgttgaatga
    38761 tatcattatg gatgctgtag caatcaatcc aacaccaaca gataaaggaa aacgcctgaa
    38821 gattttttat gctacacaag tcgctgtcaa accacctaca tttgtcgttt ttgtcaatga
    38881 agaggaattg atgcattttt cttatgaacg tttcttagaa aatcaaatca gaaaagcatt
    38941 tacttttgaa gggacgtcca tccgaattat ccctcgtagg agaaaatagg cattttacca
    39001 tatcgacgtt tttttttcaa aagaaaaaga cagtttcgtt tgttacgttt ctatgactaa
    39061 aaaaagcatg tgaaaattgg taaaaacctt gctattaagc ggttcatatg atatcgtgat
    39121 aacagaatat tgaagttaat caatatttct ttttaaactt ctggaccact cagaaagttt
    39181 taactaaatg atatttctat catatctctt gtggaggagg tgaatctatc catggcaaac
    39241 aaagcagaat taatcgaaaa agttgcttca gctactgatt taactaaaaa agatgcaact
    39301 gcagcagtag atgctgtatt ttcaactatt caagatgctt tagctaacgg cgaaaaagtt
    39361 caattaatcg gttttggtaa ctttgaagtt cgcgaacgcg cagctcgcaa aggtcgcaac
    39421 ccacaaactg gtgaagaaat cgaaattcca gctagcaaag tacctgcatt caaaccaggt
    39481 aaagcattaa aagacgctgt taaataagac agttgaaaaa gcgcttgtgg ctctagggct
    39541 acaagtgctt ttctttttat ttcctagcag aaaaggaata aataagagac agagattccg
    39601 aatccgtatg tcatacatag aaaacacatg tattttattt aatttttatt tatttccaag
    39661 ttatgaggtg agctttgctc ttatcttttc tagaatttgc ttactaatgg taaaataaaa
    39721 ataagaagag gagggatgcg tatggaactt gattatcaag tagaagatgc gttgacgaaa
    39781 gtgttttacg gagaaacgct agtcagccag ctaagagaac tttccattga tcagccaatc
    39841 ttatttttaa cgaatcagcg ctattatgat ttgtttgcag ataagatcaa cccgttattt
    39901 gcaaatcaag caaacaatga ctggtatatt tgtgccaaca ctcaatgcaa tcatttaaca
    39961 gaattaaaga acctgcttga tttcgtcaaa cgctatcccg aaaatcaatc catgtttatc
    40021 atcggatttg gaaatgaagg gatcatggat ctggcaggtt tttttcaaaa acatacaacc
    40081 cttgattcat cactatggat gatccctgta tcgatccgtt ctctaagccg agcattaaca
    40141 ccgcagcgga cgattttgca gctaccaaat cgtgtagtac tgcaaaccat caatctgcca
    40201 gaacgcatcg tttatgatca aacaatcagc gagcggcaag tggacggaaa gcagattgac
    40261 tttttgactt ttatctgttg cggaatgatt tgtgatcatc gttttttgca aaatctgtat
    40321 aaaaactatc caaataaaaa gcaattaacg actcggccgt ttgccgggat gatcgaagaa
    40381 atgatctatt tttatcaatt agaggcagaa aacctgtcaa attatggtaa actgtttgaa
    40441 caagcttttt atctgaccga aaatggccat ctactttctg ctagtatgaa aaaattttta
    40501 ggaatgatgt ttcatttcgt gtggaatctc caagcatatc cttttgattt ccaactaaaa
    40561 aacttttttg tgtggctgga cttgcttggc tatccaatcc actggctgga acagatttca
    40621 aagatggagt atttggaaaa agttcttttt ttggcagaaa aaagcaaaaa aatacttgtt
    40681 ttagaaaaaa taggtataat agacggatac caacgtccaa acgaaaaaga attattgaga
    40741 acaatgacgg tatttcaaca aatcgtcagt gagataagag ggaattgaaa tgaatacaaa
    40801 tagcgaaaaa atgttgcaag ctttatcaga tgaagacttg tcacaagcac aaatcttttt
    40861 agaaaaagca ttaaaagaag acccagcaga tatattagct gaactaggcg aagaattgct
    40921 ggctattggc tttttgatcg aagcaaaaca gatttttgaa caactgatca accaataccc
    40981 agaaaatgac ggactgaaca tcccgttagc agaaattgca atcgaagata atgagatcga
    41041 cttagctttt gaatacctgg aaaaagtgga taagcaaagt gattactatc cgcaaagctt
    41101 attggcgata gctgatttgt accaagttat cggaatccct gaagtgagtg aagcaaaatt
    41161 gaaagaagca gctgggttgc ttcctgatga accattgatc cagtttgcgc tagctgaatt
    41221 gtatttttca gttgatcgtt tcaaagaggc agcagtggtt tatgaaacat tgttggcaag
    41281 cgggataaat gaaatttcag gaatctcgat gcaggaacgt ttaggacaat ccttaagtat
    41341 gcaaggagaa ttcgaagcag ctatcgcacc tttagaagaa gcactgaaag aagaaaggac
    41401 agatgatcga ctgttccagc tagcttttac gaatcttcaa ttgaaagaaa atcagcaggc
    41461 aatcaactat ctggaagaac ttcgagaagt taatccgcat tatcaatcgt tatatctata
    41521 tctaggacaa gctcttcaag aagaagaaat gattgaagaa gcacaaacag tgttagaaga
    41581 aggaatcaaa gaaaatcctt accaagtcga actttatcac ctggcctcgg aaaatgcttt
    41641 ccgtctgcat gatagacagc gagcagaaga acttttactg aaagctttag aagtaggaga
    41701 caaacaagat gaaacattac ttactttaag caatctttac ttggatgatg aaagatatga
    41761 agatgtgata caagtgatca atcagatgga agaaactgct aatccttatg cagaatggaa
    41821 cctagcccat gcttataatg aattagaaga ctttaccgtt gcagctgtac actatgaaca
    41881 ggcttatcat gaactatcac atgaaccaga tttcttgaaa gagtatgcct ttttcttaag
    41941 agaagaaggg cagctgaaaa gagcgcaaga attattgact tattatttaa cacttgaacc
    42001 aggagatatg gaggcactgt cattattaga tgacctaaca gagaggtgag aaaaatgcta
    42061 attgatgtac gggaaaagaa gaatttttta acgtggatgg tcaatcatgt atcttttagc
    42121 cgaagagaag ttttttggat cttgaattat cttgccaatc atgaagcgat tttaagcaat
    42181 gtccactttg ttgaaggggc gaattttgcg gatcgaggac tgcagatcag tgatctatct
    42241 gttgaagggg aacccatgaa attgtttttg cagggaaaag aatttacgga cacagaccag
    42301 atttttcatg aaatccgttt gaattggaaa aagcctttat atgtggaatg tatattcgaa
    42361 aatgcttggc agacaagaga atatctatct atcttagaag acaatccatt ttcttcttgg
    42421 gattcttcag tcagtgaaga agatactgaa ttaattgaac gatattttca agaaaaagaa
    42481 gaagaggcaa aactaaaaat gctttatgct caaatcgatc aagcactaga agatggagat
    42541 agggatgctt ttttgaaatt atctgatgaa gttaaccgaa agatcctttt gcagacacaa
    42601 aagaaaagta accgggtaag tgaacataac aattagttaa aaatagtata gtactaaaaa
    42661 agacaaagat cagctggatg tggaat
//
LOCUS       DAFCNS010000017        42517 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFCNS010000017 DAFCNS010000000
VERSION     DAFCNS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.017"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            476..1339
                     /locus_tag="LNE23_000867"
     CDS             476..1339
                     /locus_tag="LNE23_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6930740.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="LNE23_000868"
     CDS             complement(1468..3090)
                     /locus_tag="LNE23_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6930741.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="LNE23_000869"
     CDS             3339..3860
                     /locus_tag="LNE23_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK6930742.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LNE23_000870"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LNE23_000870"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBK6930743.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="LNE23_000871"
     CDS             5941..6759
                     /locus_tag="LNE23_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBK6930744.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="LNE23_000872"
     CDS             complement(6862..7059)
                     /locus_tag="LNE23_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930745.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="LNE23_000873"
     CDS             7228..7806
                     /locus_tag="LNE23_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK6930746.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="LNE23_000874"
     CDS             7901..8038
                     /locus_tag="LNE23_000874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930747.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="LNE23_000875"
     CDS             complement(8851..9354)
                     /locus_tag="LNE23_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK6930748.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="LNE23_000876"
     CDS             9713..10738
                     /locus_tag="LNE23_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6930749.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11085..11963
                     /locus_tag="LNE23_000877"
     CDS             11085..11963
                     /locus_tag="LNE23_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBK6930750.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12439..12555
                     /locus_tag="LNE23_000878"
     CDS             12439..12555
                     /locus_tag="LNE23_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBK6930751.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13483..13821
                     /locus_tag="LNE23_000879"
     CDS             13483..13821
                     /locus_tag="LNE23_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930752.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13915..14145
                     /locus_tag="LNE23_000880"
     CDS             13915..14145
                     /locus_tag="LNE23_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930753.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14545..14916
                     /locus_tag="LNE23_000881"
     CDS             14545..14916
                     /locus_tag="LNE23_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBK6930754.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15190..15882)
                     /locus_tag="LNE23_000882"
     CDS             complement(15190..15882)
                     /locus_tag="LNE23_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6930755.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15885..16670)
                     /locus_tag="LNE23_000883"
     CDS             complement(15885..16670)
                     /locus_tag="LNE23_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6930756.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16907..18307
                     /gene="lacG"
                     /locus_tag="LNE23_000884"
     CDS             16907..18307
                     /gene="lacG"
                     /locus_tag="LNE23_000884"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBK6930757.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18332..18649
                     /locus_tag="LNE23_000885"
     CDS             18332..18649
                     /locus_tag="LNE23_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK6930758.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18662..20335
                     /locus_tag="LNE23_000886"
     CDS             18662..20335
                     /locus_tag="LNE23_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBK6930759.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20349..21980
                     /locus_tag="LNE23_000887"
     CDS             20349..21980
                     /locus_tag="LNE23_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBK6930760.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22852..24042
                     /locus_tag="LNE23_000888"
     CDS             22852..24042
                     /locus_tag="LNE23_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HBK6930761.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            24176..26365
                     /locus_tag="LNE23_000889"
     CDS             24176..26365
                     /locus_tag="LNE23_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6930762.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26362..28155
                     /locus_tag="LNE23_000890"
     CDS             26362..28155
                     /locus_tag="LNE23_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK6930763.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            28355..28669
                     /locus_tag="LNE23_000891"
     CDS             28355..28669
                     /locus_tag="LNE23_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6930764.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28690..29067
                     /locus_tag="LNE23_000892"
     CDS             28690..29067
                     /locus_tag="LNE23_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6930765.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            29077..29850
                     /locus_tag="LNE23_000893"
     CDS             29077..29850
                     /locus_tag="LNE23_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930766.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29872..31275
                     /locus_tag="LNE23_000894"
     CDS             29872..31275
                     /locus_tag="LNE23_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK6930767.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31457..32641
                     /locus_tag="LNE23_000895"
     CDS             31457..32641
                     /locus_tag="LNE23_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6930768.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32638..32928
                     /locus_tag="LNE23_000896"
     CDS             32638..32928
                     /locus_tag="LNE23_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930769.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32925..33146
                     /locus_tag="LNE23_000897"
     CDS             32925..33146
                     /locus_tag="LNE23_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930770.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            33188..33967
                     /locus_tag="LNE23_000898"
     CDS             33188..33967
                     /locus_tag="LNE23_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBK6930771.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            34034..34528
                     /locus_tag="LNE23_000899"
     CDS             34034..34528
                     /locus_tag="LNE23_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK6930772.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            34584..35222
                     /locus_tag="LNE23_000900"
     CDS             34584..35222
                     /locus_tag="LNE23_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930773.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            35294..35689
                     /locus_tag="LNE23_000901"
     CDS             35294..35689
                     /locus_tag="LNE23_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6930774.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            35673..38126
                     /locus_tag="LNE23_000902"
     CDS             35673..38126
                     /locus_tag="LNE23_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6930775.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            38123..40135
                     /locus_tag="LNE23_000903"
     CDS             38123..40135
                     /locus_tag="LNE23_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930776.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETKY
                     SKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSNATGYL
                     VKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYVAYTGL
                     VKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTKIVLPN
                     TDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDTNNGED
                     RENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFSQVLFI
                     IFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTMLYSLSA
                     SSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNRQTRRF
                     QRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQQGGIG
                     QRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDFKQSMA
                     DTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRKRQQTV
                     TPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            40132..41154
                     /locus_tag="LNE23_000904"
     CDS             40132..41154
                     /locus_tag="LNE23_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK6930777.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            41171..42100
                     /locus_tag="LNE23_000905"
     CDS             41171..42100
                     /locus_tag="LNE23_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6930778.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            42345..42461
                     /locus_tag="LNE23_000906"
     CDS             42345..42461
                     /locus_tag="LNE23_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK6930779.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT        13914 a         7049 c         8394 g        13160 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt atatttattc tatcaaaaaa
    10921 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
    10981 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
    11041 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
    11101 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
    11161 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
    11221 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
    11281 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
    11341 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
    11401 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
    11461 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
    11521 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
    11581 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
    11641 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
    11701 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
    11761 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
    11821 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
    11881 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
    11941 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
    12001 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    12061 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
    12121 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
    12181 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
    12241 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
    12301 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
    12361 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
    12421 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
    12481 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
    12541 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
    12601 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
    12661 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
    12721 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
    12781 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
    12841 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
    12901 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
    12961 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
    13021 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
    13081 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
    13141 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
    13201 tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca
    13261 ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa
    13321 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
    13381 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
    13441 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
    13501 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
    13561 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
    13621 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
    13681 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
    13741 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
    13801 aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa
    13861 atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg
    13921 gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa
    13981 aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt
    14041 tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta
    14101 gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat
    14161 gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt taatattact
    14221 aactttaatg cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt
    14281 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
    14341 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
    14401 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
    14461 atttctcctg gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac
    14521 tttgcggagt atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc
    14581 atcaatcaat cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc
    14641 attatttctt cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat
    14701 aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat
    14761 cttaagttta atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa
    14821 gatacggaaa cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact
    14881 cagcttggtg gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa
    14941 gtatatcaaa aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac
    15001 actattttta atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa
    15061 aagtcaaaga tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt
    15121 tcactggcag ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag
    15181 ttgaatcgtt taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa
    15241 atctttgatg gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc
    15301 gaatggatca attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga
    15361 gtaagcatct tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt
    15421 aagaaaaata tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc
    15481 ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac
    15541 agtagccgtg gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc
    15601 aagccctttt gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct
    15661 ttctttttct tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg
    15721 tttcaaaatc tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa
    15781 accatctttt gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc
    15841 caaaaacatt gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg
    15901 gtattttcgg ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc
    15961 tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca
    16021 taaaaatcat ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta
    16081 tttagagcga tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg
    16141 tgaacgccat ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg
    16201 gtgatcggtc cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt
    16261 tctggtccat ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga
    16321 attttcttgt tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa
    16381 atcgtttctc cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga
    16441 atctgcagag tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt
    16501 tgagcccctc catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg
    16561 cgaacggtca tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct
    16621 tttttattga ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc
    16681 tctaatttaa ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag
    16741 taaacattta gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt
    16801 ttatgattat ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc
    16861 agttagaaag agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc
    16921 agaagatttt atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga
    16981 aggtggcaaa ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc
    17041 cgatccggca agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt
    17101 tggtgtaaat ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg
    17161 gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag
    17221 aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca
    17281 tggagatttt ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt
    17341 tcatgaattc aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac
    17401 caatcaatat ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt
    17461 cgcctgtctt cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa
    17521 cgaacttccg ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga
    17581 cgcgccggaa gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt
    17641 agatgctacc tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg
    17701 tgaagcaaat caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc
    17761 atccacgcgt aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta
    17821 cgatggagaa aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa
    17881 agtgaaggga atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg
    17941 gctgatttat ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca
    18001 ttacaacaaa atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg
    18061 cattattatg gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa
    18121 ggcgataaca gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt
    18181 ctcatggaca aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca
    18241 gaagcgttac ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat
    18301 tatttaataa gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg
    18361 ttttgagatc gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga
    18421 agtaagacaa ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt
    18481 aacccttgca cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat
    18541 ggagatggga ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag
    18601 agatctgatt caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc
    18661 tatggatgga atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg
    18721 taatagttat ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt
    18781 ttcaagcatt ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga
    18841 atctgtctcc acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact
    18901 cgtttcagca accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa
    18961 caaagtaatc aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag
    19021 cgtctctccg attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc
    19081 ttcctttgtc gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat
    19141 taccattaaa atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt
    19201 tcctttctct ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt
    19261 ccaaatctca tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga
    19321 cgggtactta ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt
    19381 tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac
    19441 caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg
    19501 gaattttgta ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct
    19561 actctttgct aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag
    19621 tttcgctgtc aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt
    19681 tattccattt ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt
    19741 attgggcatg aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt
    19801 ggttctagga acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt
    19861 tgacttcctg atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga
    19921 agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga
    19981 agtacctgtg gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt
    20041 actttgtgct ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc
    20101 tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct
    20161 gcaggatttt gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa
    20221 agaagacacc gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg
    20281 attaacgaga gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag
    20341 gagccataat gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag
    20401 gggctttggg ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag
    20461 cagatatttc gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata
    20521 tttcaaaaga tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt
    20581 tgtggcagga tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg
    20641 aaacgacgat tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt
    20701 ttcactacag tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag
    20761 gtctgacctt tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg
    20821 aaatgaagca gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg
    20881 gaatgctctg gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg
    20941 ctgctttttt gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag
    21001 taatgctcca cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa
    21061 tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg
    21121 gttcaatgaa tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag
    21181 tgatcgtagt tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg
    21241 cttttggtga agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag
    21301 agtatttgct ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct
    21361 tcttctattg ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta
    21421 tgaattatct cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg
    21481 attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa
    21541 atctagttaa aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc
    21601 aaacctggtt tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt
    21661 ttggggacta caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa
    21721 atgttagtgg cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag
    21781 agtttgatac taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg
    21841 ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg
    21901 tttgtgaaat tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg
    21961 tttcattggt gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca
    22021 gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa
    22081 ccagaaacga tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac
    22141 tttgtattat gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa
    22201 agttgtgcag ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa
    22261 tatgatttga gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag
    22321 aacttctttt tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg
    22381 gaaccatctt atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata
    22441 gggattcccc ctccatatta tgttggctct ttattcccac tctgttttca acgtaaagtg
    22501 tataaaacag aatgcgatac tatcaaggga gttaaaacaa tatacagaac agtaaaacac
    22561 tataaataaa aataaaagtg caataaaagt gcaatttttg cacctaatca tactctgttt
    22621 atatatgaaa actagattct gaatccaata tttggttaac gagaggcgtt cgaatttgaa
    22681 cgtcttttat atattacttt caaataaata tgaggtagaa tactatctat tttcttttta
    22741 acccaattgt tataatggag agtgtaaaat attttgtgta aatagaaaaa aggaagtccc
    22801 ttctgtagaa tagagttacc acaacacatt cacagaagag aggacttccc tatgaacgat
    22861 tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga attattccgt
    22921 gtccatttgg agaaagcagt caatacgctt ctcaaaacgg agttaacggc tttcctagat
    22981 tacgaaaagt atgatcgcat tggttttaac acgggtaatt ctcgtaacgg ctcctatgac
    23041 cgtacggtca agaccgagta tggggaactt catctccaga ttccgcgcga ccgcaatggt
    23101 gagttcaagc aacagactgt tcctgcttat agacggacga atgacacgtt agaggagacc
    23161 gtcattcacc tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa
    23221 atgtatgggc attactacac gccccaaacc atgtccaata taacaaaatc atttacagaa
    23281 gaggtaacgg cgtttaatgg gcgggagctt catgaccgtt atgctgctat ttatatggac
    23341 gcaacgtata ttccgttaaa gcggaaaacc gtcgccaagg aagctattca tatcgcagtt
    23401 ggcattcgcc cggacggatc aaaggaagta ttgagctatg cgattgcacc gactgaatcc
    23461 atcacgattt gggaggaaat tttattggac cttcaagagc gcggtttgaa aaatgtcctc
    23521 ctgttcatca cggatggctt aaaggggatg gtaggagcga ttagtcggtt ctatcccaaa
    23581 gctcgttttc aacattgttg tgtacacgtt tcccgtaata tcagtcataa agtgcgtgtc
    23641 gatgatcgta aggaagtctg tgatgatttt aaaatggtgt atcaagcctc atctaaagag
    23701 gtggcgttgg aagcacgtgg tacttttgcg gaaaaatgga aaaccagcta tccaaaagtg
    23761 gttgaatcga ttctttcgaa cgatcacttg ctcactttct atgatttccc cttggccata
    23821 cgcaagagta tttattctac gaacttgatt gaatccttta ataaacaaat caagaaatac
    23881 agccaccgca aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct
    23941 tttgatactt acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa
    24001 ggcgagctcg aacaaatgct aagccaactg attgagaatt agaaacaatc tgcagtaggg
    24061 aagaatcatt tacacaaaat tattgacgct cccttataat gagaactttt actaacataa
    24121 tttatgataa acttactata taatcatatt aattaaatag ttaggagatc tgcttatgtt
    24181 tatatctact cttcaaattc gaaattttag aaattatgaa tccgaaatct ttcgattcga
    24241 taatgagaca aatactatta taggtgagaa tgattcaggg aaaaccaatg ctcttactgc
    24301 attgagaatc ttattagatg atagttatta ctatagtagt aaaacactaa aagaatcaga
    24361 tttttttcat ggaattcaaa atgggtggca agggcattgg attattatct ctgcgacatt
    24421 tgaaggaatc agcgaagaag aatttgacaa tgagatttgt gcttccattt ctttaaattc
    24481 tgaaagtcaa acaatcttag aagaattgat ttcaaatgca gataaaggtg taggtagtat
    24541 ttctctattt attagaccta acaaagctat tagaaagcaa ttatttgata tttccactga
    24601 attagactct catcaattta acgaatttag aaattctatt cgtctatccg actatgaatt
    24661 ttactacaca tctaaatcaa atctaaattt ctgtatcaat gaaaattatg ataaattggt
    24721 aggaagactc aatgacaata tagctagtaa tcctgaagaa gacgatgaag ctctgttggg
    24781 ctcaagaatt gacatgtcag atattttcaa gcatatttcg gttgtttatg ttgacgctct
    24841 tcgagatgtt cttagagaaa tgaaaaacaa tcgaaaccca gtcaaaagaa tcatggaaac
    24901 aattgaatca aaaatatcat cagataatgt tgagtcatta aaaacaatta tacagcaatt
    24961 aaatgagact attaccagtg ttcctgaaat taggaaaatt ggcgaaaata ttaatcggca
    25021 actaaatagc ataattggtt cagtgtattc tcctaatctt cttctttcct caactatgtc
    25081 agatgatatg ggttctttag caaaatttct ttcaatgaag ccagaacaaa atatcgactt
    25141 agacttacta gggttaggac atctaaatat gatttattta gctttgaaaa tagtcgaatt
    25201 tgaagcgtgt cgttcaagag aactacttaa tattatgttg attgaagaac cagaagctca
    25261 tattcatcat catattcaaa aaactctgtt cgaaggatta aatcttcaaa aaaactatac
    25321 tcagattctc atgaccacac attctgtaca tttagctgaa gtgtctgaaa tatctcggat
    25381 gaatgtatta aaatcgttta atggcaaatc tatcgttatg aatccagcaa ataatctgaa
    25441 ctcatttgct aaagaaaagt taaaaaagaa caatctaaat ctaataaacg cagtagagag
    25501 atatttagat gttaaaagaa atggattgct tttttctaaa ggtgttatct tagttgaagg
    25561 agatgctgaa gaaatactta ttcctcaaat agtaaaaaaa ttatttggta taaacttaga
    25621 cgaacttgga ataggtttga ttaatgttgg tagtacttcg tttgaatata tcgctagctt
    25681 gtttgacgat gatagaatac agagaaagtg tgctattgta actgatttag atgaacaggc
    25741 aatcccatca gaacatcgat tgtataaaag cactgcacaa gagaaaggtc aagcaagaaa
    25801 agaaaaatta tcctcccttt ttaatgaaaa caattgggtt aattgttttt atgctaatac
    25861 aacatttgaa attgagtttt tagatgtgaa taaagaaaat attctaactt atgtcagtcc
    25921 aattatagat gacgcattca aagataaaaa aacaataact gcttataaag aagaaataca
    25981 aagtgaacaa tatttagaaa gaaatttagc gatgttaaaa ctagccaatc atgttggtaa
    26041 ggggtggttt gcaattgagt taggtgaaaa aattgacttt ttaatcaaaa ttcctaagta
    26101 tattcttaat gctattgctt ttgcatgtca ggaaatagta gatctctcaa tttataaaaa
    26161 aattctactc catacaatcg acttgtacga ggaaactaca gaattgtcag ctttatcagc
    26221 tagtttaaaa acaagtgata cagctgattt gaatatagca ataatttctt tattagagta
    26281 ctttggtaaa gatgaggatg ttgtgtatct aatcaatcag atagaaaatt acacaaaaat
    26341 actttatgct gaggtttcaa aatgacaaat atcttcgaaa aaatcgtaaa tgatttaaat
    26401 ttagaacaaa aagatgttgt ggaaagtgaa gataaccttt atgtaaatgc atgtcctggg
    26461 agtgggaaaa caagggtact tacaaggaaa attgcttacc aagcaattaa acacgatgga
    26521 tcactaaaaa gaataattgc tatcacatac actaatagag ctgctgaaga aattaaggaa
    26581 cgcctaagtt tacttagtat agatgatgat attaatattt gggtggggac aatacatcag
    26641 ttttgtcttg aatttattat ttatcctttt aaaatgaatc tctcaagagt atcaaaagga
    26701 tttacaatta ttgatgatta tactcaaaga gcttacgtca gccaaattaa taatatgctg
    26761 aatttaaatc tcaaaacgta tgaactgaac aaaattgatt tgtcactttc tcatgatttt
    26821 aaaattaatg aagttcagta tactcaagtt tctgaaatgt atcacactat gttggaaaaa
    26881 aataaagaaa ttgattttga tatgatttta ctagtagcgt ataaaatttt aactgtaaac
    26941 agtaacgttg cacgtaatat tgctagtaat ataagggcga tttatgtgga tgaatttcaa
    27001 gatactagag aattacaata caatgtaata gctaaattac ttcaaaacaa ccctcaaatt
    27061 cagtcaatgt ttgttggaga tattgaccaa gcaatttatg gatcattaga tggtatagct
    27121 aaatcagttg aagaactaga aatgctaact agtcacactt ttcaatcaaa gacgctacat
    27181 ggctgttaca ggtctaatca gagattagta gacttctact ctaattttca gagttgtcct
    27241 tacgagattg aaagcagagg gaataataaa aacgaccgtg gttttataag ttatgattgc
    27301 aaagttacta aggaggattt acccaacata ataaaaaata taataaagga aaagattgaa
    27361 agcggcattc atgaaaaaga tatttgtgta cttgctcctc aacactatcc tctgttttca
    27421 ctgggtgagt atttaaagaa agaattaccg tattgcaatt ttgatgctat aaatatatca
    27481 ccaattaagg taaataatca tagtttattt ttcaagttag caattttgta ctttactgaa
    27541 tcaggtgaaa aggttcgaag aagaaagcta attgcaaacg atattttaat tatattaaaa
    27601 aatgaatttg gaatagaagt taaagattat tttattgact tagatttatt acagctaata
    27661 aacagtgtga aaagaatgta tagtggagac gacaacggtg ttgatttata caaatttgtt
    27721 accaaacata tatttgttaa tcttaatatt gacgagaaaa attacccgtc tatttttaag
    27781 cactatctat tttttattaa cgaagtagaa gatcggataa aaaataattc attatctagt
    27841 agtgtatcta gttttaagaa gatgtttaaa tctcgagatg gtatcacact tactacagca
    27901 cataaagtta aaggaaatga gtatgatact gttatcgcta ttaatcttct aaacgggaaa
    27961 attcctcatt ggaatgaaat ttttaatgtt tctgataaag gaattagttc agcgagaaaa
    28021 ctactatacg taatttgttc aagagcaaaa aataatttat ttcttttctc agaggtgggt
    28081 tattttacga gtaaaggaga cttgctttat cccactcaat tattagataa aatagaattt
    28141 gattataata tttaaaatac ctaggtagtc gttgttttaa atatagtagg aatcggacgc
    28201 tcagtacgga gcgtctattt ttttacccaa ttttgaaagg aagtgaactt atgaaaataa
    28261 aaaatcaaga atcaaaaggt cgctctcccc cctttaaaac catcacacaa ataagattca
    28321 ttcaaacata aaaacagaaa ggataggtaa aaatatggaa cttaaatatg tcattcccaa
    28381 tatggaaaaa acatttggac aattagaata tgctggtgaa gggaacattg aacaacgtcg
    28441 aataaatggc agaccaacca ttctctctcg tagttacaat ttatactcaa cgattcaacg
    28501 agcagatgat attgttgtca ttcttccctc cgaagctggc gagaaacatt ttgaatccga
    28561 cgaaaaagtc aaactcatca acccacatat taccgcagaa ggttataaaa ttggcaatcg
    28621 aggatttacc aattacatta tgcacgcaga cgatatggtg aaagcataag aaaggaaagg
    28681 tgaaaaaata tgagattagc agaaggtatt gtgattgaaa aagaccagac atttggcaca
    28741 ttgaaatttt ccgccttgcg tcgtgaggtt cgtatccaga atgaagatgg tacggtttcc
    28801 gaagaaatta aagaacgtac ctatgactta aaatcaaaag gacaaggacg catgatacaa
    28861 gtcagtatcc ccgcttctgt tcctttgaaa gaatttgact acaacgcaga agtggaatta
    28921 atcaatcctg tggcagatac cgtagcgacg gctacttttc gaggggcaga cgttgattgg
    28981 tacgtgaaag cagacgatct tgtattaaaa ggacagacaa aacctcatca gaaatataaa
    29041 aaagaagcgt ccgaaagtga aaaataggtg ttgcttatga caaacacacc attgattttg
    29101 aaagaaattc cgaaggatga agccatttct tttattcggc aataccatta ttcaaagata
    29161 ctccctagat tgtgtaaata ctttctaggg attttttcag aagaaaaatt attaggcgtt
    29221 gtggaattag gctggggtac gcaaccccta caaaccatac gcaaactctt tcccgacagt
    29281 tcccttcaaa ccacagatta tttagaaatt ggaaagatgt gttttcttcc agaaatgaat
    29341 caaaccaatt attttggcag tcaagctctt tcagctctaa ttaagtggtt gaaagaacat
    29401 acggactgtc attttttata tacactggca gatggaattg agggaaaatg tggctatgtc
    29461 tatcaagcgt ccaacttctt ttactgcggg tatttcaaga caagtgtcta tcgtgataag
    29521 cagtcatggg aaaagataca ccctcgaagt gctcgccttt tattagagga aaatgctcgc
    29581 tttgaacagg tagagaaaaa gcattggctt tcccaggcat tttgcgaata caaagggatt
    29641 gagaaaatca atggacgcat gtttcgctat ctctatccgt tgacaaaaga agcgaaaaaa
    29701 ctgttaggac acactctcta tcgacgtcat tattatccga aagaaaaaaa tttacgcttt
    29761 gaaaagcgaa tcgcttatca gaaatatgaa gccatttctc aaccaacttt tgataaacag
    29821 gctcgtatct acaatacaca attattttga taggtggaaa ggaggatttt tatgccattg
    29881 cttcatgaca gaggagagcg aatcaaagcc aacgataagc acttggtcta tcattttgta
    29941 ctaatttcat tgctggtagt ttttctagtc gtcctactcc cttttcattg gagagcgtta
    30001 caatcggtga actggcaagc agttcagtta gatacatttt tctatcaact tcatacgcca
    30061 tacttttgga gcagtattct cactgctctt tttgtatgcg gacttatcag ctggctttat
    30121 tatcactatc gtatagattc tataaaacag ttagagcacc gtcaaaagtt agctcgtatg
    30181 attttagaaa atcattggta tgaagccgaa gaaactcaat cagagggatt ctttaaagat
    30241 ttacccagca agaaaaccaa acaacgaatc cgccattttc ctaaaatata ttatcgaatg
    30301 aaagatggac taatccatat tcatgtagaa atcaccttag ggaaatatca agatcagctt
    30361 ttgcacctag aaaacaaact agaaacagga ttgtattgtg aattagtgga aaaaacgttg
    30421 aaagattctt atgtagagta tgtcctactt tatgatacta ttggcaatcg aattggcatt
    30481 gaagacgtgg tagctcaaga tggacgtgtc aaattgatgg aatcggtcta ttgggagttc
    30541 gattctctcc ctcacatgtt aattgctggg ggcactggta gtggaaaatc ctattttatt
    30601 ctcacattga ttgaatctct cctgcataca aatgcaaagc tttatattct tgaccctaaa
    30661 aatgctgatt tggcagactt aggggcggtc atgaataatg tctattaccg taaagaagat
    30721 atgttggcat gtatcaatca attttacgaa gatatgattg atcgcagtga aaccatgaaa
    30781 caacacccta actataaaac aggagaaaat tatgcttatc ttggtttgtc tgccaatttt
    30841 cttatttttg atgaatatgt ggcaatgatg gatatgctag gtcgtgaaag taccaccgtt
    30901 attcataaac tcaaacaaat tgtaatgtta ggacgacaag caggcttctt tttaattctt
    30961 gcctgtcaac gtccagacgc aaaatatttt agtgatggga ttcgggacaa ctttaacttc
    31021 cgtgtggctt tgggtcgtat gtctgaaatg ggctttggta tgatgttcgg aagcgataca
    31081 caaaaacaat ttttcttaaa acccatcaag ggaagaggtt acgtggatac aggaaaaagt
    31141 gtgattagtg agttttatac accccttgta ccaaaaaggt acgatttctt aggagaaata
    31201 ggaaaagtca ttcaaaaaaa gcaatctgag cctgtaccgc acgaagtgaa aggtacaggt
    31261 tcagacttgc tttgattggt gtggcgttag ccacgccaat cctttagccc cctctatcta
    31321 acaggggggc acaaatacaa ctgaaaacag gcaattctca tcaaccaatg ttgatggtac
    31381 aaaggattgc ggaaaatatg aaaggtgtaa actcaagggt catagtttac atctttttta
    31441 gattggaggt ttgcgaatgg aaaacgaaaa aatttggatt aaggaattga aagaaaagcg
    31501 actggcttat ggcgtttctc aaaataaact ggcggttgct tctcatatta ccagacctta
    31561 cctatcggat attgaaacag gcaaggcagt ccctactcaa caggtgaaag aagatttact
    31621 gaacgctttg gaacgcttca atcccgataa cccattggag atgttattcg actatgtacg
    31681 gattcgattc cctaccaatg atgtggctca aattatcggt gaggttttac gtttgaatat
    31741 ggattatatg ctacacgaag actttggcta ttattcttat cccgaacact atcgtttcgg
    31801 tgatattgtt gtcctagtct ctcatgatgt aagtaaaggt gttttattag aactgaaagg
    31861 aaaaggctgt cggcaatttg aaaactttct attggctcaa catcggagct ggtatgattt
    31921 ctttcaagat tgcatggaac ataaaggtat tttcaaacgc ttggatttag ctatcaacga
    31981 taaaacaggg attttgaata ttccagattt ggcaaagaaa tgtaaacagg aagaatgcat
    32041 ttccgtcttt cgaagcttta aaaattatcg cagtggtgaa ttggttcacc gtgatgaaaa
    32101 accatacatg ggaaacacac tttatattgg ctcattaaaa agtgaagtct atttctgtat
    32161 atatgagaaa gattatgagc aatacgtgaa aaatgatgtt cccttagaag acgcagaagt
    32221 aaagaatcgg tttgaaattc ggttaaaaaa tgaccgagcc acccaagcca tgaaagattt
    32281 attggcacat caacaggcgg aaaaaacagc ctttgaaatt atcaatcgct atatccgctt
    32341 tgcagataaa gacgacacta aacgtcggag tgattggaaa actaatgaac gttgggaatg
    32401 gtttatcggt aaaaatcgtg gagcattacg cctaaccaca cagccagagc cttattcttt
    32461 tgaacgaaca ttgaactggt tacatcatca agtcgcccct acattaaaaa tcgcaagcat
    32521 tttagatgtt ctcaacggta caaccattat ctccaccatg attcaggaag ccaaactgac
    32581 agaaaaacat gagaaattaa ttgaacaaca acatctagcc atggaggatt tgattacatg
    32641 agccttttaa caaaagaatt aaagaagtta gggtttcagt gtggcattga gtttcaagcg
    32701 tatatacaaa atacagggaa atatacgtct ctcattattg agggaaaacg gcaagctggt
    32761 gataccatct atacctatga tttttacaag gtgagatttt ataataatta taccaatcga
    32821 gtaaccgtct atggagagca tttaacccct tttcaattat taaggcgtgt aaaaagctat
    32881 atctattatc gagaaaaata tcttaaggaa aggaggacaa tcacatgaat tttgggcaga
    32941 atttgtacaa ctggttcttg gataatgcac agtcattggt attgttagcg attgttgtca
    33001 ttggactgtt tttaggtttt aaacgagaat tttcaaaact cattggcttc ttgattattg
    33061 ctctaatcgc cgtcggactt gtgttcaatg cagctggcgt gaaagatgtg ttattaaact
    33121 tatttaaccg aatcattggt gcttaacaga tatttttcat gataaaatag gaagtatata
    33181 ttttgttatg aaaggattta tgaatatgtt tacaaatgat caatttaaat ctcttttaat
    33241 taaaacatta gaaagaaaga gctcttcaaa ttattatgaa ggaggtaacg agattgtttc
    33301 taaaatgaaa atatcagagg taactttaga tgagactgac gattttactt attataaagg
    33361 atataaaaag ggctggaata cgctttgcac atatcttaaa ataagagttc cttttgatga
    33421 tttagacttt tttgaaagtc ataaagattt gattacccag acagcaagct ccatatatga
    33481 taaacaaggt gataatgtat tagtggatac tattttagta ccacttccag agaattacga
    33541 agtaattaac tttagtcagt tgaaaattag tgatgtggtt tcacaagcta ttgaagacgc
    33601 tgagtctttt atgagtaatg gtgaatatca acgtgccttt gacagagttc acactgcttt
    33661 tcatggatat ttaattgaaa tactaaaaaa atatgaaatt acagttccta gagacgaaaa
    33721 tctatctaaa ttatatagta ggatacaaca attaattgaa aaagaaattc aacctactga
    33781 acttgctgat atagtgaaga caacgataag gtcatcaaat ggaatgatta gctctcttaa
    33841 tgaagcaaga aataggcatt cacttgcaca ccctaataca aatattattg gaaagagaga
    33901 agcaaaacta ataataggta tttcaagtac agttacagat tatattagcg gataccttga
    33961 taagtagcgt cattaactga ttgctacttt ttttatgact atttttataa aaaggatgtg
    34021 ataagaattg gaaatgaaag tctatgtggc aaatttagga cggtataatg aaggtgaatt
    34081 agtcggagca tggtttactc caccaattga tgaggaagaa atggcagaaa gaatcggttt
    34141 aaatgaggac tacgaagaat atgcaataca tgattttgag cttccttttg atgtggacga
    34201 atacacgccg attagtgaaa tcaaccgtct atgtgaagcc attcaagaaa tcgaaggtac
    34261 acctatttat aatgaactca aagaaattca aggcatgtgg tttagtagct tggaagaatt
    34321 actggaaaac aaagaggata ttcattgtta tagtgattgt gattcgatgg aagatgtcgc
    34381 ccgttattac gtggaagaaa caggacagct tggtgaagtc ccttccaatt tacaaaatta
    34441 tattgactac caatctttgg gacgtgatat ggaaatagag ggtaattacc tagttacctc
    34501 tcatggggta tttgaatatt gtcagtaagt ttaacaatcc gtgttataat taaattaata
    34561 aatagcacgg agaggtataa attatggtaa atgtatttga aaatcttaaa ctattaaaac
    34621 tagacatgaa agataaaggc tgggtgatag attcctttta ctttcgttac aaacagcaaa
    34681 attatattgt acttgttaag ctatttgaaa aagaggaaaa agttccagaa tatgctctgt
    34741 taaaactaga atttttaaag gaaaacgatt tttcagatat gctagctgtc tatgctaatt
    34801 cggtaaaatt atatacagac acgaaaacaa taagagaata ttttggtatc gaatacagta
    34861 gtaacttggg agatgtttta tttcaattta gccaaactct tgctcgattt attccgacag
    34921 aagttagtga gaagaaaaat gaagaccaaa aagaagccat gtgtttctca ttaagtcaaa
    34981 gtgattcaga agatcccaga aaaaaatatt gtttttcagt tagaagaaat cctttaaagg
    35041 gaaatggtga gctgggaaaa cgaagtcctt ttaatgacaa taaaacgaga ttgtatagac
    35101 ccagcttata tgaacgatta gggtcagata ccaatttaag tttcagatat tcaatgaatc
    35161 cagatgatga agaaactgat gaaggtatta ttgcaaaatg gacaaaaaat aaaattgaat
    35221 agttaaagca ttgaaagagc aatcacctat tttgattgtt ctttttctat ttttacggaa
    35281 aggacgtgaa gacatgaaaa aaataagaag ttacaccagt atttggtcag tagaaaaagt
    35341 gatttatgcg attaatgatt tacaactccc tttcccaatt acctttacgc aaatgacatg
    35401 gtttatcgtt tctttgtttg tcgtcatact ctttggcagt ttaccaccac tttctctgat
    35461 tgacggagca ctactcaaat acttagggat tcccattgtt ctcactgggt tcatgtgcca
    35521 aaagaccttt gacggaaaga aaccctatgg atttctaaag tctatcatta cctatgctct
    35581 acgccctaaa gtaacctacg ctggcaaaca aatacgatta aaaaaagaaa caatgaatga
    35641 atcgattacg attgtaagga gtgaacaact agatggcata tccgattaaa tatatggaag
    35701 ataatctcgt atttaatcat gatggcgaag tattcgctta ttatgaatta attccctata
    35761 attacagttt tttaagttcc gatgaaaaaa ttcaagttca tgataatttc tgtcaattga
    35821 ttgcccaaaa tcgtgatggc aagattcatg ctctgcaact agcaaccgaa tcaagtattc
    35881 gagccacaca ggaacaatcg aaaaaagaaa tcactggacg attaaaagag atagcttacg
    35941 aaaaaataga tgaacagacc gaagcgttaa tttctatgat tggtgacaat caagtagact
    36001 atcgcttttt tattggtttt aaactctacc tcaatgaaca ggaagtatcc gtaaaaaatg
    36061 ttgggaaaga aattcaaaaa gcatttgcgg aattctttca tggtgtgaat cacaaattaa
    36121 tgggcgattt tgtttctatg cctatggcgg aaatcgaacg cttttctaag atggaacagt
    36181 tgttagaaag taaattatcc agacgctttc agattcgtaa attggagaaa gatgattttg
    36241 gttatattat tgaacatctt tacgggcaaa caggtgtccc ttttgatgac tacgactacc
    36301 atttaccgaa acgatacgaa gataaaaaag tgttggtaaa gaaatacgat attctcaaac
    36361 ccactcgctg tttaatcgaa gaaaatcaac gctatttacg gattgaacag gaagatacca
    36421 ctacttatgt ggcgtatttt acaattaata gtattgttgg tgaattggat ttccctaata
    36481 gtgaaatttt ttattaccag caacagcagt ttactttccc cgttgatacc agtatgaatg
    36541 ttgaaattgt cgccaataaa aaagccttat ctaccgttcg caataaaaag aaagaactga
    36601 aagatttaga tagccacgca tgggaaagcg acaacgaaac cagtactaat gtggtggacg
    36661 ctttagaatc tgtcgatgaa ttagaaagta atttagacca aaccaaagaa tctatgtaca
    36721 aactaagcta tgtggtacgg gtatcagcaa agaatttgga cgagctgaaa caacgttgtg
    36781 atgaagtaaa agatttttat gatgatctgt ctatcaaatt agtacgacct tttggcgata
    36841 tgctaggctt gcataatgaa tttattccct caagcaagcg atatatcaat gactatattc
    36901 aatatgtcac gtcggatttt ttggctggtc ttggtttcgg tgctacacaa atgattggtg
    36961 aaaattctgg tatttacgtc ggttacaatc tcgatacagg cagaaatatt tatctccaac
    37021 ctagccttgc aagtcaagga attaaaggct ctgtaacaaa tgcattagcg tccgctttta
    37081 ttggttcatt aggtggcgga aaatcattct ctaacaatat gctggtctat tattcagtac
    37141 tctttggcgg acaggcaacc attgttgacc caaaagctga acgtggtgaa tggaaagaaa
    37201 ccttacctga tattgcccat gaaatcaata ttgttaatct cacgagtgag aacgacaata
    37261 aagggttact tgacccttat gtgattttaa aactcccgaa agattcagaa agccttgcca
    37321 ttgatattct cacgttctta acaggtattt ccagccgaga tagcgagaag tttcctgtcc
    37381 tgcgtaaagc tattcggagt gtaactcaaa gcaaacaacg agggttgtta ttggtgattg
    37441 atgaattgcg aaaagaagat acgcccatca gcaatagtat tgctgaccat atcgaaagct
    37501 ttgtcgatta tgattttgcc catctgttat tcagtgatgg cagtgtcacc caatccatta
    37561 gcttggaaaa acaactcaat attattcaag tggcagattt ggtcttaccc gattccaata
    37621 gctcttttga agaatacact accatggaat tattaagtgt ggcaatgctg attgtcatta
    37681 gtacctttgc tttagacttt atccactctg accgttccat ttttaaaatt gttgaccttg
    37741 atgaggcttg gagtttttta caagtggcac aaggaaaagc tctatctatg cgattggttc
    37801 gagcaggacg tgccatgaat gcaggggtct atttcgttac tcaaaacgcg gacgatttac
    37861 tagatgaaaa gatgaagaat aatattggct tgaaatttgc ctttcgttcc actgacctag
    37921 tggaaatcaa aaagacctta gaattttttg gtgtggacaa ggaagatgaa aacaatcaga
    37981 aacgcttacg tgaattagaa aatggacaat gtttagtttc agacatctat ggtcgtgtag
    38041 gggttatgca gtttcacccg atttttgaag agttgcttca tgcctttgat accagaccgc
    38101 ctgtgcgaaa tgaggtggaa gaatgacaca aaaacaaaag aaatttcttc actatagttg
    38161 gataacgctc ctttgtgtgg gcgttatttt acttttatta ggcacgctgg gcaatgcagt
    38221 acaagcgaca ggcttagttg atgaaaccaa atactccaag tatggtttga atcaatatca
    38281 gcttgattat tatgtggaca atagctgggg ctggcttcca tggaattgga gtgatggcat
    38341 aggacaatcg gtgatgtatg gattgtatgc cattactaat tttatttgga caatttctct
    38401 ttatctatcg aacgccacag gttatttggt gaaagaagcc tatgccttag atttcattag
    38461 tcaaacatcc caagcaattg gcgaaaacat gcagacgatt gcaggcatta caccgaatgg
    38521 cttttccagt tcagggtttt atgtcggttt cctccttctt tttattctca ttgttggaat
    38581 ctatgttgcc tatacaggat tggttaagcg agaaacgacg aaagtgatac gagctgtttt
    38641 aaactttgtc gtggtgttta ttctatcggc ttcctttatt gcgtatgcac ccgattatat
    38701 tgcgaaaatc aatgattttt ccaaagatgt cagtaccgcc agtctgaata ttgggacaaa
    38761 aattgttctt cctaacacgg atagtcaagg gaaagacagt gtggacatga tacgtgacag
    38821 cttattctct gttcaagtaa agcaaccatg gctattgctt caatatggct ccactgatgt
    38881 agaagcgtta ggggaagaac gtgtggaaag tttattgtct accagtccag atacaaacaa
    38941 tggtgaagat agagaaaatg tagtgattga agaaattgaa gatcatgaca atacctattt
    39001 aaccctaccg aagacgattt ccagattagg aacagtcttc tttttgtttg tattcaatat
    39061 tggtatctcg atttttgttt tccttctaac aggaatcatg attttttcgc aagtgctatt
    39121 tattattttc gctatgtttt tgccaatcag tttcctatta agtatgttgc cgagttttga
    39181 tggcatgggc aggcatgcca ttaccaagtt atttaatgtg attatgacaa gggctggtat
    39241 taccttgatt attaccgttg cctttagtat ttccactatg ctttattcgc tgtcagcaag
    39301 ttctcccttt ttcatgatta tgtttttaca aattgtgacc tttgcaggga tttattttaa
    39361 actgggcgat ttaatgagcc tattcacttt acaaagcaac gactctcaaa acatgggcag
    39421 acaagtgttt cgcagacctc gtcaaatgat gaatcgacaa accagaagat ttcaacgcag
    39481 tatgagtaag atttttcgtg gaaaagcagg gtcaacgaag caaaagacaa tgacaacaaa
    39541 gcagaatcaa accaataaag ctaaccatac tcgtcaaaat gaacggacta caccaaagaa
    39601 agaaacagca ctttccaatg tgaaaaagca acaaggcggt atcgggcaac gattaggtga
    39661 aaagacagcc catgtattgg acacgaaaaa tcgtctagtc aataaaagaa agcaggcaaa
    39721 acaacagata caggcgacac caacccatgt caaatatgcc cttcatcaag gaaaagagaa
    39781 tgtcactcat aatgtaacag actttaaaca atctatggca gataccaaag ctcaacgcaa
    39841 acaacagcgt atgaaccagc aggagcaaaa acgaaagaac attgctcaaa agcgtttgga
    39901 gctggataaa gcaaaagaaa aaagacgaaa agaaaaccca tcatctcctg ttcagaataa
    39961 ccagcagcat gttcgcaagc gtcagcaaac cgtcacgcct gttttgtcac aaaacactaa
    40021 acgtgaaaat catcagcgac cattgaaaaa agagcaaaaa caaaagacac caccgaagca
    40081 attcaatcag aggaagctaa ataaaagaac ggataagcga ggacaacaaa catgaagcta
    40141 aaaataacag tgatttgtag tgtggtcttc tctctcttct tttttctctt attattccta
    40201 gtcattttct ttgcagatga tacggacagt ggcgaaaaca acaaggattc ttctattcca
    40261 caaggcggtg tgaccgtttc accagaagtg ttggcacacc gtccgttgat tgaaaaatat
    40321 ggtaaggaat atgggattga agactatgtt tcttatatct tagccattat gcaagtggaa
    40381 tcgggcggta cagcagaaga tgttatgcaa agttccgaat ctttgggttt accgcccaac
    40441 agtttgagta ccgaagagtc catcaaacaa ggggttaaat atttcagtga attattaacc
    40501 agtgcagaac aacaaggagt ggatatagat tctgttatcc aatcttataa ttatggcggt
    40561 ggctttttaa attatgtgag gagtcatggg aaaaaatata cctatgaatt agccgaacag
    40621 ttttctaaag agaagtcggg tggtcaaaaa gcagactatc ccaatcctat agccatacct
    40681 gtgaatggtg gttggcgata taactatgga aatcagtttt atgttcagtt ggtttcacaa
    40741 tacctaaccg acacttcccc aacagaattt gatgatgaaa ccgtgcaagt cattatggac
    40801 gaagccttga aatacgaagg gtttccctat gtctttggag gggcgtcgcc taccacctct
    40861 tttgattgta gtggcttaat acaatgggtg tatgacaaag ctggcatttc tctccctcgt
    40921 gtcgcccaag atcagtatga cgctacgcaa gaaatctcta tggaagaagc ccaagcaggc
    40981 gaccttatct tctttcattc aacctataat gcaggcacat atgtgactca tgtggcgata
    41041 tatttagagg gaaatcgctt ttatcatgca ggcgacccaa tcggttatgg cgacttaagc
    41101 agtcgttatt ggcaagacca tctgattggg gctagacgtg ttattcataa ctaagaaagg
    41161 aatgaatcaa atgattataa aaatcgagcg aaaagaaaag aaggagaaac caaagaaatc
    41221 taaaaaaccg agcaagcaaa gaaaagttcc gatgattaaa gttggcacgc ataagaaatt
    41281 aacctttgtt ttatgggttt tattgattgg tagtgtcgga tttggaatct ataaaaattt
    41341 tactgccatt gacactcata ctgtccgtga aacagaaatc attaaacagc agattgtcga
    41401 taccaaccaa gtagaaagtt ttgtgaaatc ctttgcgaaa gactactttt cgtggcaaca
    41461 atcccaagaa gccattgata aacgaaatga aaaattgacc cattatttga cggaagaatt
    41521 gcaggtatta aatgaagaaa tgattcgtaa ggatattcct actagttcta gtgtgaacga
    41581 tatacaggtg tggcaggttt ctcaagtgaa tgagaacacc tttgaggtac tgttttctgt
    41641 tgaacaagtt attaccgaag acaaagacaa ggaaactata tcgtctagct ttcatgtggt
    41701 tgtccatata gatgaatcag ataatatggt tattatcaaa aatccgacaa tgagtaagaa
    41761 acctcaaaaa tcggactacc aacccaaaca gctagaaagt gaccatacag tagatacaga
    41821 aacgatggac gaaatcacta gctttttaga aacgttcttc cagctctatc ctaccgccac
    41881 agaaaaagaa ttgacttact atgtaagtaa tcatgtgtta cctatgatta acaaggaata
    41941 tgttttcgag gaattggtga atcctatctt taccagaaaa gataaccaag taatcgtgaa
    42001 tgtcgctgtg aaatatttag atcaagaaac aaaggcaacc caaatctcgc aatttgagtt
    42061 gatattagaa aaacaagata attggaagat tgtaaaataa caaatattgg tacatgatta
    42121 cagatacttt gtaatcatgt actctttttg ataaaaaatt ggagattcct ttacaaatat
    42181 gctcttacgt gctattattt aagtgactat ttaaaaggag ttaataaata tgcggcaagg
    42241 tattcttaaa taaactgtca atttgatagc gggaacaaat aattagatgt ccttttttag
    42301 gagggcttag ttttttgtac ccagtttaag aataccttta tcatgtgatt ctaaagtatc
    42361 cagagaatat ctgtatgctt tgtataccta tggttatgca taaaaatccc agtgataaaa
    42421 gtatttatca ctgggatttt tatgcccttt tgggtttttg aatggaggaa aatcacatga
    42481 aaattattaa tattggagtt ttagctcatg ttgatgc
//
LOCUS       DAFCNS010000018        42357 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFCNS010000018 DAFCNS010000000
VERSION     DAFCNS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.018"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..554)
                     /locus_tag="LNE23_000907"
     CDS             complement(<1..554)
                     /locus_tag="LNE23_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBK6930780.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIE"
     gene            complement(708..1238)
                     /locus_tag="LNE23_000908"
     CDS             complement(708..1238)
                     /locus_tag="LNE23_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK6930781.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(1337..1687)
                     /locus_tag="LNE23_000909"
     CDS             complement(1337..1687)
                     /locus_tag="LNE23_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBK6930782.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1844..3373)
                     /gene="ahpF"
                     /locus_tag="LNE23_000910"
     CDS             complement(1844..3373)
                     /gene="ahpF"
                     /locus_tag="LNE23_000910"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBK6930783.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(3383..3946)
                     /gene="ahpC"
                     /locus_tag="LNE23_000911"
     CDS             complement(3383..3946)
                     /gene="ahpC"
                     /locus_tag="LNE23_000911"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBK6930784.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(4090..4752)
                     /locus_tag="LNE23_000912"
     CDS             complement(4090..4752)
                     /locus_tag="LNE23_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6930785.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4936..6582
                     /locus_tag="LNE23_000913"
     CDS             4936..6582
                     /locus_tag="LNE23_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK6930786.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            6573..7655
                     /gene="uxuA"
                     /locus_tag="LNE23_000914"
     CDS             6573..7655
                     /gene="uxuA"
                     /locus_tag="LNE23_000914"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK6930787.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7763..9316)
                     /locus_tag="LNE23_000915"
     CDS             complement(7763..9316)
                     /locus_tag="LNE23_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6930788.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            9464..10753
                     /locus_tag="LNE23_000916"
     CDS             9464..10753
                     /locus_tag="LNE23_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6930789.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEAAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(10879..11958)
                     /locus_tag="LNE23_000917"
     CDS             complement(10879..11958)
                     /locus_tag="LNE23_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6930790.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(12067..12507)
                     /locus_tag="LNE23_000918"
     CDS             complement(12067..12507)
                     /locus_tag="LNE23_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6930791.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(12564..12974)
                     /locus_tag="LNE23_000919"
     CDS             complement(12564..12974)
                     /locus_tag="LNE23_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK6930792.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(13155..13589)
                     /locus_tag="LNE23_000920"
     CDS             complement(13155..13589)
                     /locus_tag="LNE23_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6930793.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(13689..14537)
                     /locus_tag="LNE23_000921"
     CDS             complement(13689..14537)
                     /locus_tag="LNE23_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6930794.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            14926..16692
                     /locus_tag="LNE23_000922"
     CDS             14926..16692
                     /locus_tag="LNE23_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBK6930795.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            16794..18053
                     /locus_tag="LNE23_000923"
     CDS             16794..18053
                     /locus_tag="LNE23_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6930796.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            18200..19501
                     /locus_tag="LNE23_000924"
     CDS             18200..19501
                     /locus_tag="LNE23_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6930797.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            19504..20355
                     /locus_tag="LNE23_000925"
     CDS             19504..20355
                     /locus_tag="LNE23_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6930798.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            20368..21183
                     /locus_tag="LNE23_000926"
     CDS             20368..21183
                     /locus_tag="LNE23_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBK6930799.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            21334..22500
                     /locus_tag="LNE23_000927"
     CDS             21334..22500
                     /locus_tag="LNE23_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6930800.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            22730..23377
                     /locus_tag="LNE23_000928"
     CDS             22730..23377
                     /locus_tag="LNE23_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK6930801.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            23461..24405
                     /locus_tag="LNE23_000929"
     CDS             23461..24405
                     /locus_tag="LNE23_000929"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBK6930802.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            24540..25991
                     /locus_tag="LNE23_000930"
     CDS             24540..25991
                     /locus_tag="LNE23_000930"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBK6930803.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            26012..26848
                     /locus_tag="LNE23_000931"
     CDS             26012..26848
                     /locus_tag="LNE23_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK6930804.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            26848..27507
                     /locus_tag="LNE23_000932"
     CDS             26848..27507
                     /locus_tag="LNE23_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6930805.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            27633..28322
                     /locus_tag="LNE23_000933"
     CDS             27633..28322
                     /locus_tag="LNE23_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930806.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            28328..29284
                     /locus_tag="LNE23_000934"
     CDS             28328..29284
                     /locus_tag="LNE23_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBK6930807.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            29328..29840
                     /locus_tag="LNE23_000935"
     CDS             29328..29840
                     /locus_tag="LNE23_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6930808.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(30096..30644)
                     /locus_tag="LNE23_000936"
     CDS             complement(30096..30644)
                     /locus_tag="LNE23_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6930809.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(30733..31914)
                     /locus_tag="LNE23_000937"
     CDS             complement(30733..31914)
                     /locus_tag="LNE23_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBK6930810.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(32150..33658)
                     /locus_tag="LNE23_000938"
     CDS             complement(32150..33658)
                     /locus_tag="LNE23_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBK6930811.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            33817..34008
                     /locus_tag="LNE23_000939"
     CDS             33817..34008
                     /locus_tag="LNE23_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930812.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            34211..35128
                     /locus_tag="LNE23_000940"
     CDS             34211..35128
                     /locus_tag="LNE23_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBK6930813.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            35121..36194
                     /locus_tag="LNE23_000941"
     CDS             35121..36194
                     /locus_tag="LNE23_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930814.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            36342..36860
                     /locus_tag="LNE23_000942"
     CDS             36342..36860
                     /locus_tag="LNE23_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930815.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            36841..37233
                     /locus_tag="LNE23_000943"
     CDS             36841..37233
                     /locus_tag="LNE23_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBK6930816.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            37309..38244
                     /locus_tag="LNE23_000944"
     CDS             37309..38244
                     /locus_tag="LNE23_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBK6930817.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            38377..39156
                     /locus_tag="LNE23_000945"
     CDS             38377..39156
                     /locus_tag="LNE23_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK6930818.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            39149..39466
                     /locus_tag="LNE23_000946"
     CDS             39149..39466
                     /locus_tag="LNE23_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK6930819.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            39710..41320
                     /locus_tag="LNE23_000947"
     CDS             39710..41320
                     /locus_tag="LNE23_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6930820.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            41477..42325
                     /locus_tag="LNE23_000948"
     CDS             41477..42325
                     /locus_tag="LNE23_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6930821.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13960 a         7642 c         8109 g        12646 t
ORIGIN      
        1 tgttcaatca aaacaagcgg taaagaagca ttgctagaag cttcccccgt tagaaaagat
       61 aggctgaaac ttagaactaa aacgactaaa atgtcatcta ccacagaagc acccaaaatt
      121 gttgatcctt cttttgtatt cactacgttt agttctttta atacttctac tgaaatactg
      181 acagaagttg ccgcaagaat aatcccaaag aaaatcgctt catttcccga aacttggaat
      241 gctttgcctg tcaaccagcc aaaaacaacc gggaagatga tcccaagcaa agcaacgaac
      301 ataccaggtc ggaaatattt tttcaaaaga gataaatcac tttccagacc agctaaaaac
      361 attaataaga tcacaccaat ctccgaaaag tcatggacta gaatatcagg tttgacccaa
      421 ttcaatccag cttggcctag caaaacacca acaagtaatt gtccaatcac tgctgggata
      481 cctaatctcc gcgagatatg tgagccaatc gttgttgaaa tcaatatcag gcataaaata
      541 ccaataaatt ccattctcat cattcctttg tataaaatat agcccgaact tagcataaca
      601 tacattattg aaaaaatagg aaaatatact cacaaaaaaa ccaacaatcc aaaagatttt
      661 tttggattgt tggtttttat acggtctatt ttaataacta aaaatgatta acgtatagtc
      721 atttttctta ttctgatgaa attgataatc tcctggatgc ccatgaaaat caaaataaag
      781 ccgaatattt ggaatagaag catcaaactt ttgaatggat tgaacaataa gacgaatccg
      841 ccaatcaaca acagaatact actataaaca ctccaacttt tagcttttgt attcatgctg
      901 aagaacaact ggaacaagct gttgatgata acagctaatc caagaagaat cggcaggata
      961 gaaacgattg caggtgcaaa agcatagacg atcagagcta agatcaggaa aaaaagacca
     1021 ctgaacagac cgatactcca agaacctgtt ttccttttaa aacggtaagc ctcaaaaaga
     1081 ttcaacaacc caagcaaaac aagataggcg gtgatcagat aaccgataaa atgaaaaaca
     1141 gcagaagggt ttagcacaat cgcaatccca gaaataatat aaacagcagc tcttaataag
     1201 gcgtattttt gaatattttg tttaatcgtc tcaaacatat aagaaaacct cctttgactt
     1261 attttaacgt gaactagtca ttttaccaaa ttaaatgtta caaaatgagc gaaggagaag
     1321 aatatctctg gaaaaatcaa cttctgagga tcttttccat ttttttgcct tttgctagct
     1381 catcgatcat tttatctaaa taacgaattt ctttcatcaa gttatcctcg atgtcttcta
     1441 ctcgtacacc acaaacaaca cctgtaatca aggaacgtga aggattgggg acaggggctt
     1501 ccgcaaaaaa ttgttcgaaa gtcacttgtt tttgtagttg atattctagt tctgtttggg
     1561 aataacccgt cagccaacaa atgatttcat ctacttcctc ttttatacga tcttttctct
     1621 ccgcttttgc tacatacata ggataaacgc ttgcaaaact tgtcgtaaaa atacgatttt
     1681 gcgccataat cagtcacctt tctttttttc aaattattta ctacttatag tatataagaa
     1741 aaagagaaga aaaagcgatt attcaaaaaa acaaaaagga ttgtgacaaa attcttgtca
     1801 ccatcctttt ttccgctctc tgacttttta caatcttcat agctcaagtg cgcatcagat
     1861 aatcgaatgc tcctaaagct gcagtagctc ctgaacccat tgaaacaatg atttgtttgt
     1921 atggactatc tgtacaatca ccagctgcga aaacgccggg gatattcgtt gcgccatttt
     1981 tctcggtcac tatttcattt cttggattca tttcgatcgt tccatctagc caatccgtat
     2041 tagggagtaa gccgactaga atgaatactc ctgaaacagc tagtgtatgc atctccatcg
     2101 tacggcgatc gatataggag agagattcta cctgattcgt accgctgatt tctttcgtcg
     2161 ctgcatgttt gataacagtg acattcgcaa gagagactag tttatcctgt aagatttgat
     2221 ctgcttttaa ttctggtaag aattctagaa catatacatg ttttgctaac ccagccaaat
     2281 cgattgctgc ttcaacacca gaattaccac caccgataac agcaatctct tttcctttaa
     2341 ataaagggcc gtcacagtgt gggcaataag caattccttt atttttaaac tctttttcgc
     2401 ctggtacatt gatagaacgc cagtgcgcac ctgtcgcgat gatggcggtt tttgctttaa
     2461 gtgatatacc gttagctaag ccaatattaa taaaatgttg ctctttattt aaggaaacgg
     2521 cctgttgatt catcataatg tcgatagggt aactacgcac atgactttct acttgctgca
     2581 tcaattttgg accttctgtg taagaggtac caatcatatt ttcaatccct agtgtttcaa
     2641 cgacttgtcc gccgaaagaa tcagcgacaa gtcctgtacg gattccttta cgtgctgcat
     2701 agatagctgc actactagct gctggtccac cgccaattac taatacatca taaggttctt
     2761 tttcaaacaa ctcttcttca atcaaagggc cagtgatttt ttcaacaatt tgttcgatcg
     2821 tcatgcgtcc gctactaaat tcttctccat ttaaaaacac agttggcact gccatgattt
     2881 ttttatcatc gatttctttt tggaacatcc caccttcaat catcgtatga gtaatatttg
     2941 gatttaatac agctaatata ttcaatgcct gtacaacgtc ggggcaatta tgacaagtca
     3001 agctaacata actgtcaaaa tgcaatgttt gtgttatttt tttgatttta agactcaaag
     3061 attcactgat tttcggtgcc cgtccactaa tttgtaacat agccagtaaa aaagaggaaa
     3121 attcatgacc gagggggata ccagcaaaaa cgattccact ttctttgcca actggattga
     3181 tagaaaaact aggtgtgcgc atcgaatctc ttttttccca agagatttta ggagacattt
     3241 ctgctatttc cttcgtaaat tcagctagtt tttgactatt ttcactatta tccaaactaa
     3301 cagaaaaaac aataggagat tcaatcagtg ctaaatattg ttcgagattt tgtttagttt
     3361 ctttgtctaa catatccacc acctaaatct ttccaacaag atcaaaactt ggtttcaatg
     3421 tttcgccact ttctttccat tttgccggac aaacttcacc tgggtgggta cgtacatatt
     3481 gagcagcgcg aatcttatca atcagtgcac tagcgtcacg accgattcca tctgcattga
     3541 tttccatagc ttgaacgatt ccgtctggat caataataaa tgttcctcgc tgagctaatc
     3601 cttgctcttc atccaaaaca tcaaaaatac gagaaatctg atgagacgga tcaccgatca
     3661 tcacgtattc gattttaccg atagcatctg atgtatcatg ccatgctttg tgagtaaagt
     3721 gggtatcggt agagcaagaa aagacttcaa cacctaaaga tttcaatgta ctgtattgtt
     3781 cttgtaagtc ttctaattca gtaggacaaa caaaagtaaa atcagctgga tagaagcaaa
     3841 gaatactcca gttccccata agatcttttt cactgacttc taaaaattct ccttgatgat
     3901 aagcatgtgc attaaattct tcgatttttt tcccaatcaa tgacatagta aaacctccaa
     3961 taataattat ttatttataa ttattgtaat ttagttggag ttgcaaagtc aatggatatt
     4021 ttgttattta ataaagatta ctcaaaaacg aaggttaatt ataaaaatag aagaggtcgc
     4081 aagaaaatgc tactgctgga aatactcggg ataacgttga gtaacagttt cctgttcttt
     4141 cagcatcatg tgtaagtgca atcccgtttg aaatcgagct tcttctggtt ttttatcaac
     4201 taaagccttg agtaaatcat aatgttgttc catgatcgtt tcccatggaa gcccctgaat
     4261 ggtcactcgt aaccagcgga aacgttctaa atgggtatta tggtcatcta accagttcca
     4321 aacttctttt cttcctgcaa tttcgaaaca agcttcatga aataaattat ctgcacggaa
     4381 aaaagcgcgt tcgtcattta ttttgactgc tttttcttgt tcttctaaaa tcgtttctag
     4441 cactattcgt gtttgtgtgt ccaatttgga acaacattca atcatgattt gctcttccaa
     4501 ctgttctcgg acaaagcgag cattttctgc agaaacaaga ttaattttgg aaacatacgt
     4561 accactttga ggtatcgtat acacgagttc ttgctgacgt agttgaatca atgcttcgcg
     4621 aatgggagtc cggccgatag caagatgttc ctccagtgcc ttttcagata ttttccttcc
     4681 aggttctaag tcagcataga taatttgttt acgaatcgct tgataagctt gtgattgcaa
     4741 gctgcttttc atctttattc ctccaaaatc atttctatga acgtagtata acagaagagg
     4801 tctgaaaaac aatctggtat gtcacattaa ctagtaaatt tgtataaata gtaaaatttt
     4861 ggttgacata gcgattttta ttgtataaaa ttcacttgaa tacaaaaact aatatgttag
     4921 tttaggaggg gaaaaatgat tgatttaaac aactggagga actttatccc tgaattatct
     4981 caaaaagaag tagaaatacc tcattttgac cgtgcatcat taagagaaaa tggacaaaaa
     5041 aatcctgtct ggatccattt cggtggcgga aatctttatc gaggatttca tgcagaaatc
     5101 gctcaacgtt tagcggatct aggcgaattg tccactggtg tcattgtttg tgaaacattc
     5161 gatgaagaaa taattgaaag ggcttaccaa gattttgatg atttgattct tgaagtggtc
     5221 atgccaagca gtggagagtt cgtcaaacgt atattagctg cgacagctga cagtctgtat
     5281 tgtcatccag attgttctga acactttgat aagatgattc gacactttga aaatcctagt
     5341 ttacaattag ttacttttgc tattactgaa aaaggttatc gattgaaaga tacaaataat
     5401 aaatatatag gaccgattgc agtcgatata aaaaatggtc caaagaagcc atcacataca
     5461 atgagtattc ttgctgcgct actttataaa cgttttttat ctggagcttg gccgattgca
     5521 cttgtctcta cagataattt ttcacaaaac ggtagtcaat tacgtacttc tttgctaacg
     5581 atcgcaaaag aatggcaaaa ggaaggttat gtacaaaata catttatcca atacttatcg
     5641 aatgaagaaa ttgttgcgtt tccatggacg atgattgatc gtatcacccc gaatccttcc
     5701 ttgtcagtta gcaaaatgtt ggcaaaagag ggcttttcaa atatgtcgat tttccaaacc
     5761 gcaaaaggaa ataatattgc tccatttgtc aatacagagg acatccatta tttaatcatg
     5821 gaagatcatt ttcccaatgg acgtccgaaa ttcgaaaaag ctggggtatt cttgactgat
     5881 cgtgcaacgg tagataaagc agatacgatg aaggtgacga cttgccttaa tcctttgcat
     5941 acgtctttag ctattatggg ttgtttactg gggtatagga gcatagctga ggagatgaaa
     6001 gatccagatt tatcgctgct agtttctgaa atcgggtaca atgaaggcct cccagttgtg
     6061 gagtctccag gtatcattga tccaaagatc tttctggaag aagtaattac tgtgcgcttt
     6121 tctaatccaa atattcctga tatgcctcag cgaatcgcca cggatacctc tcaaaaggtg
     6181 gcgattcgtt atggtgagac gattaaaaaa tatatggaaa tagaagttaa agacccgcag
     6241 acacttgtat tcataccatt gactctcgct gcttggcttc gataccttat tggcattgat
     6301 gatcaaggaa aaccattcat cccgagtcca gacccactat tgacagactt tcagcataag
     6361 ctttccgata ttcaactggg tacgcaagat acatcacata ttcatgagaa aatggctcct
     6421 attttgtcaa ataaagaaat ttttggtgta gatctttacc aagcagagct tggcagaaaa
     6481 atagaagaaa tgttcatcaa gatggtagca ggaccgaatg ccgtacgaaa aacattacag
     6541 gattacttac aagaacatgg aggaaattta tcatggaaat gacttttaga tggtacggat
     6601 cacaggaaga aaagattacc ttagatgaaa tcagacagat tcctgcggtc aaagggattg
     6661 taggcacatt gatggatata ccagtaggag aggtatggcc cagggagagg atcaaagaac
     6721 taaaagaaga aattgagtct cgtgggttaa cactaaaagt aattgaaagc gtcaatgtgc
     6781 atgatgccat taaaattgga ttaccttccc gtgagcagta tatcgaaaac tataagcaga
     6841 caatccgaaa cttggcagaa tatggaatag aagtcatctg ctataatttt atgcctgtat
     6901 ttgactgggt gaaatctgac cttgactacc gcttagaaga cggctcatcc acgttagctt
     6961 ttatatctga tgatattcca gatgatccaa gagaaatcgt cgcgcgcatc gaacattcca
     7021 gcaatgaatt tgaattgccg ggatgggaac ctgagcgctt agctcatatc aaatccttgc
     7081 ttgaagccta tgcatcagta gacgaagaaa aacttcgaga aaattttgcc tattttctcc
     7141 aatcaatcat cccaacatgt gaagaggtag gggtcaaaat ggctgtacat ccagatgatc
     7201 cgccatactc gatttttgga ttacctcgaa tcgtaaagaa tcgagaagat ttagattggt
     7261 tgtgccgcat agtagacagc ccttataacg gaatcactct ttgtacaggg agtattgcag
     7321 aagatccaga aaatgatgta tatgggattt tagcagaatt ttgtcaaaga gatcgaatcc
     7381 catttgcaca tgttcgaaat attaaatttt tagctaatcg agatttctat gaggcaccgc
     7441 ataaatccga atacggttca ttagacatgt accgcatact aaaaacaatg tatgacaacg
     7501 gatttaacga atacatccgt cctgatcatg gaagaatgat ctggaatgag acaggaagac
     7561 caggatacgg cttatatgat cgagcattag gtgctgttta tctaaatgga ttatgggaag
     7621 caatcgaaaa aggaaacaaa agtaaagata attagtgaaa aaaactgtaa ttttattatg
     7681 taaataagaa ggatgttcaa attcttgata cataaatccg gaggttgtta ataagcattc
     7741 ggatttatgt atcttatttg gtctaggaag taacttgatt ttcttttttc gattgatcca
     7801 tgagtgcttc ttcttttaat tcaaagatat attctgagat cctcataatc acttttttcc
     7861 agtcttctgg tgtgatgatt ccttttaaaa tatagatttt tttgatatga gaaggcaatt
     7921 gtttcaaagg gcagtcgctg ataacgatgt tgcagttctc cataaaatca ttttgtattt
     7981 tgatcgattc atttgaaaaa atttgttcta tcttaacgat cagatcttta ctgatataaa
     8041 aatcacgcgt atagttcaca ccaatcacaa ttcgcggctg ttttttgtag cgatcataca
     8101 aatgtactag gtcttcaacc atccattgca cattttcctt ttctaaaata actggtaaca
     8161 agtgttgatt ttgttctctg aaatccatat aaaattgttc aatggcggtt tggacttctt
     8221 tcaacacgct atgttcttga taaagcatga aagtaggaag gggcgtattt ggttgattga
     8281 agttaagaaa tccattataa agtttatttc gtgcgaatgc caatgagtaa agaatctttt
     8341 ctttttcagg tataaacaaa tcaaatacag tacaaaagga ctccaccatt tctcttgaat
     8401 aaagaacatg aggaacgttt gcttttttta aaagtttgta ttgctccaag cttgtttctt
     8461 ccgattcgat tttcgtgata gaaagtcgag ctaaaatatt cactaagatg cgttgttcgc
     8521 gacaaattgg cagattaggt cgcaaaatat ctacagaggg atcaataaaa aagtcaatat
     8581 attcatactc tgtgaattct ttttgtacgt ctttcacaga agttgcagga aaattctctt
     8641 tacatatttt taatagaaaa tataatttat ctatcatacc gctatttagt cggtcaagta
     8701 cttctttttt aaaaaaagta ttcatcataa aagccactac ttgcggatct tcatcataaa
     8761 attctgtagc agtacttgaa aagatgttcc aataaatatc taatgctgca tattgaattt
     8821 gtacctcttt tccagaaaaa gactttctgc cattattaga aaaggagata gacaaaccgt
     8881 acaatgctaa aaatgcatta atctttttca ttttcgtata caaataagag gcactatagt
     8941 ttgtttgctg acttaaactg ataatcgaat ttgtctgaca gctgaacaaa gaagtcaaga
     9001 tctgataact gactgattcg ttgcagtatc ttccaaatag acgataatag cagtaagccg
     9061 gatcaattcc ttcaaaattc aactttaaaa aaggtttttg cttatcaata tgcagttctg
     9121 ttagattgag acgctcaaag tcttgttcta attctttgat gtatagtaaa aaatttgatt
     9181 cagacatatg gaaatgatct gctaaatatt gtgtagtaat aaagaaacga tgttcagata
     9241 ggaaaagaaa aatatctaat tttgtattgc tttttttctt caagagaaac gttttaatcg
     9301 cttcttgagt ttgcatgaaa gaggctcctt tttacttaaa ttgttaaaac ttgaaaatat
     9361 tactgttttt ttcataaatt tagacaaaaa aacagtaaac atataggtag aatctctact
     9421 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
     9481 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
     9541 agaggagata gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
     9601 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
     9661 agcgaccatc aaagcaaaaa ttgactattc aggagactta gcacctatac tacaatcagc
     9721 tgttacattt gagaatgttg atcccgcttt gtcgattggc tatgacgaga acactttgac
     9781 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
     9841 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atcatttaac
     9901 gccgtatagt cagcggcaga caattgagca agattcagct atcgactcag gaggagatct
     9961 tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga
    10021 aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac
    10081 tcaaccagag ccagacaatg aatcaaaacc tgaaataacc cctggagaaa aaccagatgt
    10141 agacccagaa gaaaaaccgg atgtgactcc agaaccagat acggattctg gaaaccaaac
    10201 agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa aaccagaggc
    10261 agccccagaa gaaaaaccag atgtaactcc agaaccagat acggattctg gaaaccaaac
    10321 agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa aactagaggt
    10381 agatccagaa gaaaaaccag atgtgactcc agaaccagat acggacgcaa gagaccaagg
    10441 aataccagaa aaaataaaca aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa
    10501 atcaaatcta gcaattctaa aaataaatga agagcagctg aataaaaaat cacgaatatt
    10561 tgatagcgcc caatcagctg aaaccttaaa aagtagcaag gatacaacct ttgctagtcc
    10621 tgaaacgaaa aataaacagc taccaaaatc cggagagtca caaaacaaag ttatactgtg
    10681 gagcggaatc attctattaa gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa
    10741 cagatctctc taacatttga ataatgtctg tgaacgcatt tttcaaataa aaagaaatat
    10801 ccagaaaacg accccaaaat accagattga aatctgatat ttggggtcgt tcatacttta
    10861 tctttcttca tatttatttt aagggatatc tgtttctttt ggaagatggt tcgggatctt
    10921 tttcactttc aatacatcta aaaagaaggt aaagatcgga atgccgacaa tcaagcccca
    10981 aacgccaaag aaccgttcgc taacaagtaa gaccacaaat gtataaaaaa tcggcagttc
    11041 tgttttgctg gacatcaact tcggattcag cacataagac tcaattagat ggacaatcaa
    11101 aatcgtaatc aggatataaa caacatctcg gatgcctcct tgggaataag ctatgaaaga
    11161 tagaggaata caagaaatga taacacctgc tactgggatc agacttaaaa tgaaaatcat
    11221 gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa
    11281 cgtgtttaaa agagcgataa caaattgagc ttctaatacc aaaccaaatg tattgacaaa
    11341 cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa
    11401 tcttgagaat acaattgttt ttttcttttc aatcataaaa aagaaactta agatgaatga
    11461 catagcaaac gccactgcca tttttcctat atcctgtaaa tagccaagcg caatcgaagc
    11521 gccattttga agctgttcaa aaaaatcgtt tcgctcaata taagtatgga tatattgtaa
    11581 aacaggatct gcatcttttg gctgatgctc gtaaaaatca ataacggact ggatcatatc
    11641 aaatgtttga tgaataagca tcgggatata ctttgtgaat gccagataga taaggtaaac
    11701 gatcagtgta taaatcatga tagctaaaac acctgtaggg attcggacaa atcgctgaat
    11761 gatttgaaca agatgaatca tcaaataagt aaagataaat gtgagcaaaa tggttgtaat
    11821 catacttcta actaagaaaa gaacaaaaac gacaaataat aaaacagtga gacggcgtat
    11881 tttttcattc gccaaaaaac gctgataaac ggtcaaatcg acaacctcca aataacaaaa
    11941 aataaacacg ttattcatta tatgatggaa tatgaaaata agaaagagaa agagcaaaaa
    12001 gaaaaaccgc taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta
    12061 gcagtttcag ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg
    12121 ttgcagtgta tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat
    12181 accttctgct tgtttttgca acgcagcacc ttgaggagtc agcgtaacat ttacgattct
    12241 ttcatcaccc gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa
    12301 gggagtcagc gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt
    12361 tttcttttcc cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt
    12421 gaggagggaa gtgtagtgtc taatgatttc cttagaggcg gcatataaag gaaaacatac
    12481 ttgattaact aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc
    12541 atagcggatt tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt
    12601 ccagctgtag cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt
    12661 tcttccagag aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt
    12721 tcactttctg aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt
    12781 tgctgtttga tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat
    12841 tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat
    12901 gtacgatcgg gattatgaac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa
    12961 tacatttttt tcataaaatt accaccttta attatatttt atacaattga attttacaca
    13021 atataattgt ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga
    13081 aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa atatcagaga caaaaactaa
    13141 tctcatcata aacattacat atatttctca aaaagttgtt ttctttcttc aaaacttact
    13201 tcacgatgga cctgatgaag catccataaa taaccaaaag gatctctgaa cattgagtta
    13261 gtcacaccaa agtccatcat ttctgtcatt tcttgaatct ctacacagcc atttcgtaat
    13321 gctttattat gtgtgtcctc gatatcagag acgatgatat tgaaccagat cgtatttgga
    13381 tgttcagcag taggagcagt caaaccaaga gacgtgtttt catccagcat atggaatctc
    13441 gtgccataca aggtaaaaat gacttcatta ttgcctctag ggtaatctgt tgcctcaatc
    13501 agttgtattt catcaaagat tgtttgatac agttctagtg ctttcaaact atcatttaat
    13561 acaaaatcta tttccgttcc tttatgcata aagatctcct ctccttttca gtcgaacagt
    13621 tactagattc ttttattata ccagttaaag aaaacgcatt caaaaaaata gcggatttac
    13681 ttaccaactc attcgattgg atgataagta aatccgcgtc ccattacttc tgctacgctg
    13741 ctgacaatga caaaggcact tggatcgatt tcttctatca atggtttcat tcgggaaaat
    13801 tcatgatcag tcacaacgat caataaaacc tcttttgact ttcgattata cccgcctttt
    13861 gcctctagca gggtaaggcc acgtccaatc tcagcactta agcgaaggcg gatgtcttcc
    13921 aaataatgtt cactcatgac cataacagat ttctttcgat tgatacctgt ttcgatataa
    13981 gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat aaagaaactc ttctattcca
    14041 aaaacaaaca gattaagaga aacaacgaat gcgtctgtaa aaaataagcc aatcgaagta
    14101 ttcaagtgga aatatttttt aaaaatgagg ggagggatcg tcgtaccgcc gctagaagag
    14161 ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg cacttccaaa aatgacagac
    14221 aacaaccgat ctgaagtaat cattgtttct ggaactattg caatgattaa aggaaaaaac
    14281 aagcttccga aaagtacttt aaaaaagggt tttttaccta aaaatagaag agctagtacc
    14341 aacataacga tattcaacac taaaataact atagctcggt caaatcctaa aacagcttcc
    14401 agtaatattc ctagaccgct gacacctcca gcagcaatat gatgaggccc taaaaacata
    14461 ttgatagcga ctcctaatat gaagagggca aaaataatct taatgtatgt ccatataaat
    14521 tgtctcgttt ctttcattta tccacttcct taattcatta ataatactct tatcttatta
    14581 taacttactt ttcaccttaa gttaacttta attaataaaa aaattaggct agtctaaatt
    14641 attatgtttc attcttctag aatatacttc tacgttaaaa actgttaaaa aacttttact
    14701 ttagaaaatt ggagaaattg atcgcttaag ataaattcag gacactaaat agtacaggaa
    14761 gtagattaaa acttttaaaa gttttaagtc tatctatttg gcaagtttaa aaaataaaaa
    14821 ttcggtatca aaaaactgtt accgataaca tttttgaaag cgttgtcttt tgtttctcta
    14881 tcacttatag tgatgacgtc atattaaaac acaaggagga aatcaatgga tacagctgca
    14941 atttatcatc gaccggaaag tgaatatgca tttctatata cgaatacaag atttcgtatt
    15001 cggcttcgta ctaggaaaaa tgatatcaaa aaagtatatg tgctttgtgg agatccatat
    15061 accataacaa ctgaaaaatg gtatcaaaaa caacgaccaa tgaaaaaatg tctaagtacc
    15121 aatgttcatg attattggga aattgaagtg acgagcgaaa caaggcgctt acagtatgcg
    15181 ttccatgttg ttggggaaga taacacggac tgtttttacg gtgatcaagg aatttttcct
    15241 tatcgagaaa atgtgattac tgaaccaaat ttttattttc gaatacctta ttttcatcaa
    15301 atcgatcgat tcactattcc agaatgggta aaagaaacag tatggtatca aatttttccc
    15361 gaaagatttg caaacgggga caaagcaaat gaccccgaac acactttacc ttggggaagt
    15421 aaagatccag accgagaaga cttcttcggg ggagatttgc aaggtgtgag ggatcatttg
    15481 gattacttga cggaccttgg cgtcaatggc atctattttt gtcccatatt taaagccaca
    15541 tccaatcata aatacgatac gattgattat tatgaggtgg atcccgcttt tggcgataaa
    15601 aaacttttaa aaaacttgat tgatgaagca cataaaagag ggatacgtat catgttcgat
    15661 gctgtattca atcatatggg tgtccattca ccacagtggc aagacgttct caaaaacgga
    15721 gaaaaatcga tctataaaga ctggttccac atccgttttt tcccagtaga ttcttatcag
    15781 atgactgact taccagaaac tgctgaaaat atcccatatg atacttttgc ttttactcca
    15841 ttcatgccga aactaaatac agcaaatcca gaagtgcaaa aatatttgct ggatatcgca
    15901 acttattgga taaaagaatt cgatattgat ggatggcgtc ttgatgttgc taatgaagtc
    15961 gatcatcatt tttggaaaaa attcagagaa gcagttacag aaatcaaacc agacatctat
    16021 atccttggag agatttggca ttcctctcaa gcttggctgc aaggggatga attccatgca
    16081 gtgatgaatt atgcattcac agattcgatt aaagattatt ttgcaaaaaa gaaaatcaca
    16141 gcttcacaaa tggtcagtgg aatgaatcat cagcaaatgc tttacagaga tcaggtgaac
    16201 gaaggaacat tcaacttatt agattctcat gatactgcca ggatcttgac cctttgccaa
    16261 ggtaacaaag aattgatgaa gtctgtgatg gcctttatgt ttcttcaaaa agggtctcct
    16321 tgtatctatt acgggacaga aatagggatg actggtgaag atgacccgga ttgccgcaaa
    16381 tgtatgatat gggagaagga ggaacaagat ctagagctat tcggttttat aaaagaactt
    16441 gtttctctaa ggaaacagtt gtctaagatt atttctgaag gttctactca gtggctaata
    16501 gtatatgatc gtgaagataa gctctatttc acacgggaac tagagggaca gatcatctat
    16561 gtttacttta atcagtcaaa agaaccttgg gtagtagaac aggaaaatga ggtgattctt
    16621 agtcaaaatt gtcagttgtt agaagacggg aaagcagaga tacgacaata cggatttttg
    16681 atcatgtgtt gaaaactgtt accggtaaca acaagaaagc gtttgcaaat gaagatttgt
    16741 gcccattata ataagggtgt aacagcacgt tgaatacaca ggaggaaatg aagatgaaaa
    16801 aaagaagtgt gaaacagttg ggtttagggt taatctcttt aggaatctta ggcggattag
    16861 cggcttgtgg caatggtaac tcttcagcag aaggggaagc cgatgaagga aaaacgttaa
    16921 cagtttccgt tgattctgga tataaagatt atataaacga aataaaaaac actttcgaaa
    16981 aagaaaacga tgtgaaaatc aagctagtag aaaaggacat gttcgatcag ttggaatcat
    17041 tagcgttaga tgggcctgct ggaaagggac ctgacgttat gatggcagca tatgaccgga
    17101 tcggtgcgtt aggacagcaa ggacatctgg cggaagtaaa actgggaaat gaagcagctt
    17161 acgatgaaac agacaaagcc caagtgactt atgatggaaa aatctacgga gaacctgcag
    17221 taatcgagac ccttgttttg tactataaca aagatttagt cgatacagca ccatcaacgt
    17281 ttaaagattt ggagaatcta tccaaagaca gtcgttttgc ttttgagtca gaagcaggaa
    17341 aaaatacagg atttttagct aaatggactg acttttatta ttcctatggt ttaattgcag
    17401 gttacggtgg ctatgtattt ggtgatgatg gcacaaatcc ttcagatatc ggtttgaata
    17461 atgcaggtgc tgtagaagga atctcttatg caacagattg gttcaaaaat gtttggccaa
    17521 agggaatgca agacataaaa agtgcaggag acttcgccag ccagcaattc atgtccaata
    17581 aaactgcagc aatcattgac ggtccatggc aagcgcaaac atacaaagaa aacaatatca
    17641 attatggtgt agccaaaatt cctactctaa ataatggaca gccttatcaa ccgtttggcg
    17701 gaggaaaagg atgggtagtc agcaattatg ctaaaaacaa agacctctct caaaaatggc
    17761 ttgattatgt aacgaatcag gaaaatcaag aaaaattctt tgaaatggta aatgaaatcc
    17821 ctgcaaacca acaagctagg gaaacagcaa aggctaaaaa cgatgaatta acaacagctg
    17881 tgattgaaca atatgaaaca gctcaagcga tgccaaacat cccagaaatg ggcgaagttt
    17941 ggacaggtgc agaaaacttg atgttcgatg cagcatcagg ttcaaaaaca ccaaaagaat
    18001 ccgcagatga agcagtaaaa acaatcagtg aagccataga acaaaaatac tgattaaaat
    18061 gataaccgtc catttttata aaaaatggaa caggtgcttc agataaaagt ggcaagagcg
    18121 agatggaaaa ttatcttgct cttgttgcca ttatcttaaa agcagtttgc ggtttagcaa
    18181 gtttagaggg ggcattcaga tgcagcaaga taaaacaagc aatatcaacg aaaaaaaaat
    18241 cacgctttgt tcacttattc cagggatggg gcagtttgta aatggccaag cttttaaagg
    18301 tatcgttttt ttcgccatat tcgtcctatt cattggtcag atgcttctgg gcggatttca
    18361 tgcattggta ggattggtta ctttgggaag tataccaatg gaagatcatt cattatttat
    18421 tttgattgaa ggcactttgc aattcatcat tacaatcatt ttcttgattt tttacggttt
    18481 gaatctttta gacgcttatc gagtagcgaa gatgcgtcaa gagggcaaaa aaatcaatcg
    18541 gacgatgaag gaagtcatac ataatatagg tgatgaaggt tttccttatc tattgacact
    18601 tcccgcttat cttctcatga tttttacgat cattttccca gtcctagtta cattgttcac
    18661 tgcatttact aattatgact tcaatcatat tccaccagcc agtttaattg attgggtagg
    18721 ggctaaaaac ttttttagta tcttcttttt aagctcatat cgtaatactt tttttgcagt
    18781 cttttcgtgg accgtgattt ggactttatg tgcaacaact ttgcaaatca cccttggtat
    18841 tctcacagcg gttgttgctc accagaaatt tattaaattc aaacgtattt tcggtatcat
    18901 tttcttatta ccatgggcgg tcccagcttt tattactatc atgagttttt cgaatatatt
    18961 caacgatagc gtaggtgcca tcaatactca agtgattcct ttcataaacc agcttccttt
    19021 tgtagatatc agtgcaatct cttggaaaac ggacccgaac tggacgaaag tagcaattat
    19081 cttagtgcag ggctggttag gatttcccta tatctatgtg atggttacag gaatcttaca
    19141 atcgatttca gaagacttat atgaagcagc aaaaatcgac ggagcaaatg catggcagcg
    19201 cttttcaaac atcacgcttc ctgctgtctt cctagtggca gcgccaacat ttattacaca
    19261 gtatacgggg aatttcaata atttctccat gatctatctg tttaacaatg gtggtcccgg
    19321 aagcgtaggc ggaggagctg gttctactga tattcttatt tcctggatct ataaacttac
    19381 gactggaact tctccgcagt attctatggc ttcagcaatc acgttgatta tctccgtcat
    19441 cgttatctgt gtgtcattgc tggtatttaa aaagacaaat gcgttcaata tgggggatta
    19501 acaatgaaaa aatttcattc gagtgctcga ttccaaaaac gactttctca cttttttact
    19561 tacttttttc ttttaatact gtcagtcatt atcatctatc cgcttttgat cacagcaagt
    19621 actgccttca agtcaggaaa tattgcagct tttagtttag atctttcaag tgactggact
    19681 ttaggaaact ttactcggct attcagtgaa acactttacg ggacttggta taaaaataca
    19741 ttgatcattt ctgtcctaac aatgatcgta caagtgactg tagtaacttt ggcgggctat
    19801 acttatagtc gctatcgttt tatcggccgc aaatcaagtt tgatgttctt tttagtgatt
    19861 cagatggtac cgactatggc agcattgact gccttttatg tcatggcttt cttattagaa
    19921 gcgctcgata agtactggtt cttgacaatg atctatatag gcggtggaat tccaatgaat
    19981 acttggctga tgaaaggata ctttgacact gtaccaatag atttagatga atctgctaaa
    20041 cttgatgggg cagggcattt caggattttt ttccagattg tattaccgct tgtcaaaccg
    20101 atgctcgccg ttcaagcatt atgggctttt atgggacctt ttggggattt catgttagct
    20161 aagttcctgc ttcgaacacc tgaaaatcag actgttgctg tcggcttaca aacatttatt
    20221 tctgatgtga caaaccaaag agtctctctt tttgctgctg gggcaatttt gattgcacta
    20281 ccgatttgtc tattattctt ctttttacaa aagaactttg ttaccggcct gacatctggc
    20341 ggaacaaaag gataggagca gagtatcatg caaacatttc cgttaaacta tttttccgca
    20401 ttacgtacgc caactcaagt gtttgcggga cgcaaactat tatcatggcc taaatttttc
    20461 ttgatatttg tatttttagt ttccttgatg gtcatgcctg tgactctttt ttatgccaat
    20521 cagatccaag cgattccatt agaacaattt ctatccgttc attcgctgat cgatgaacaa
    20581 ggaacccaaa aattttctga acttgaatta tctgaaactg ggttacaagc atcgcaacaa
    20641 acgatagctg tgacccctga aattttggta ggtgtgtctt tatctgagaa acaacaatcc
    20701 gatcatggaa cttttattga ttttgagaag gaacaatggg tgatccaaca aaaggataaa
    20761 tctggtatac gccgctatac gatgaattat agcccatctt tccaaccaga ttctgtacgt
    20821 acgcccgaag atttccaacg atttttagaa agagaatttt atgccagtaa tcgtccaaca
    20881 atcattttat cttatagttt aagtcttggt cttgtcctat ttgtcatgac gagtctcatt
    20941 ttattcgggg cttccttttt cttgtggatg acaagaaaaa gccaactttc atccattcat
    21001 acattcaaag agagtgcaaa tttgatgctg aatgttatgg gaataggttc catggcagca
    21061 gcagttgtag ggtttatcca ctttgacttt atcttgatgc taagtgtcca aacaacgata
    21121 actgtcttgc tgcttttatg ggtttttgtc aaaacaagat tcaaagataa gcgtgtcgaa
    21181 tgacaagttt aagcgattta tgcgacaatg aatgtgaaaa aaatgaaaaa taaagtagtt
    21241 tttgaaaaat ttctgaaaaa attaaacttc ttagttgatt cttttctttt ttagtattaa
    21301 cgtaataggg tagttttttt aaggaggaaa atcatgttag agaaaacttt atacaatcaa
    21361 ttattttcac gttctttctc gctccctgtc gaagtaaatt attgggacgg tacaacgaaa
    21421 caatacggag atacggacac tcctccccaa atcaaaatca atatccatga agaaatccca
    21481 atgaaagaga ttacaagtaa cgcttctttg gcactaggag aagcctatat ggatcatcga
    21541 atcgagatcg agggaagtat ccaagcattg atcaacgatg cttatcagca agcaaacagc
    21601 tttttacgag gaaaagatta tctaaaatgg ctgccaaaga aagaaaaaca tacgaaacaa
    21661 aaaaacaaag aagatatcca tgcgcattat gacttaggta acgactttta taaactttgg
    21721 ctagatccaa ctttgactta ttcttgtgct tatttcaata cacctgaaga tacgttggaa
    21781 caagcacaaa tcaataaagt ccatcatatc ttagacaaac tctttatcaa agaaggcgaa
    21841 acgttgcttg atatcggttg tggctgggga acattgatgt tcactgctac caaagagtat
    21901 aatgtcaagg caactgggat cacactaagt gaagaacaat acgatttgat taccaaaaga
    21961 ataaaagaag aacatttaga agataaatgc cgtgtattat taatggatta ccgcgaacta
    22021 aaaggcgaaa cgtttgatca tatcacaagt gtaggtatgt tcgaacatgt aggttctgaa
    22081 aatcttgaag gttatttcaa agtggtaaaa gatttattga aaccaaaagg aacagctttg
    22141 atccatggaa tcagccgaca acaaggcggt gcgacaaatg cttggatcaa taaatatatt
    22201 ttccctggcg gttatgtccc tggtgttact gaattagttg ataatattac aaaaaacgat
    22261 ttgcaactga ttgatttgga aagcttacgt cgtgactacc aattgacatt agaacattgg
    22321 acaaagaatt ttcatcaagt ccaagatcaa gtcacgaaag aaatgggtga ttccttctat
    22381 cgaatgtggg atctatattt acaagcttgt gcagcttcat ttgaatcaag taatatcgat
    22441 gtgattcaat atttgcttgt tcaacctgga aacaatgaca tcccaatgca tcgtgcataa
    22501 ttgaggcaaa agtactgttt aaagactatt taaaaagaag ctaagtttta tgatgctcca
    22561 gaagtgaact tttttgtcta acttctagga ttgtctctaa agcttagttt ctttttcttt
    22621 atattttcat aataaaagtt tggttcttta atttttaaac atgacagaaa attttttctt
    22681 cgttatactt gtaatagaat actattttat aacaaagggg atcgaatcaa tgaaaaaaac
    22741 tgttatttat actagtttat ttgcagcttt gtttttagct gcttgtcaat caaataccaa
    22801 tcaaggcaca tctgggaata cgtcttccag tagcgaacag cgctcttcac aggtatcgga
    22861 ctcctcaaca aaagcaagca catcttcgtc tagccagaca aaaagttcta gtgaagcaag
    22921 tactcagtca gatatcaaag tgtctgcagc tcgtgcgatc gaattgttcc aagataaata
    22981 cccagatact gcaattacta gtttggaatt agatagtgat tggggatctt acttctacaa
    23041 gatagaaggt gtcgatgatc aaaaagaata cgaagcaaga attgatgctc aaaatgaaaa
    23101 aatggagaca gagaatccag aaactctaga tcgagatgaa caaaatggtc aaaagaaaaa
    23161 agaagatggt ctggatgttg ataaattgat cacgattgag gaagcagcaa aaatcgccgt
    23221 cgacaaagta ggaagcggta cggcaacaga ttgggatttg gataaagatt taggtactac
    23281 ttattgggaa gtcaaagtaa aagacggcaa tcaaagtaca tcagtcaaga tcaatagcca
    23341 aacaggcgaa gtactgtcaa ctgaacaaga tgattaagaa tttcttcgga aaacaagagt
    23401 tgatttttct tttagtcatg atacactaga caaaataact tgtttgaagg agcgatttac
    23461 ttgagtttag aaacgacctt tgccaatcat atctttgcca atcctttgat gaatgcatct
    23521 ggtgtccact gcatgactac ccaggaatta gatgaactgg ctcattcaga agcaggagca
    23581 tttatcacta agagctgtac gatcaatgaa cgaaaaggaa atcccgaacc acgctatttt
    23641 gatgttcctt tagggagtat caattctatg ggacttccaa acttaggttt ttcttattat
    23701 ctagagtatg ctcttgcgta tgaaaaagtg caggaaaacc aaaatcaacc gttatttttt
    23761 tccattgccg ggatgagtgt ccaggaaaac ttggaaatgc ttgagaaaat agaaaaaagt
    23821 gggtttaatg ggatcactga attaaattta tcttgtccca atgtgcctgg aaaaccacaa
    23881 ctagcttacg attttgaggc cacttatgaa acactgaaag aagtattcag catcttttct
    23941 aagccgctag gaatcaaatt gcctccttat tttgattttg ctcattttga tcagatggct
    24001 gatatcttga atcagtttcc attgacctat gtcaatgcta tcaacagtgt gggcaatggt
    24061 ttgtatatcg atacagaaca agaagcagta gtcatcaaac caaaagaagg gttcggcggc
    24121 attggcggag agtatatcaa gccgactgct ttggcgaatg ttcgcgcttt ttatacacgt
    24181 ttgaaaccag aaatccaaat cattggtaca ggtgggattc ggacaggaca agatgcattt
    24241 gaacatctgc tatgtggcgc aagcatgtta cagattggta cagagcttca taaagaaggg
    24301 ccggaaattt tttctagaat cataaaagaa ttaacgcaga ttatgtcaga aaaaggatat
    24361 acgtcaattg acgaatttaa aggcaaatta cgcaccattt catagcaaag agacgaaggt
    24421 catgattgat cttcgtcttt ttttataaaa aacactcgtc tgttcattcc tgtgcaagcg
    24481 tactcattgt atgttagacg aacttgtggg aaaataacag aggcaaaaag gaggataata
    24541 tgaatgatgc aacagaatta gcaaaagagc ttaaagaaaa agaatttagt tttcaagaac
    24601 tagttacgga tatacagaaa aaaataaaga agcaaaatca tgaattgaat gcgtttgtcg
    24661 cgcttgaacc attcgagagt aattcccacg agctattgat tccccagtca gaaaaagggc
    24721 cattggctgg tatcccattt cctttaaaaa tgctcggtca agaaaaaaag ggatggctag
    24781 ctacttcagg atctcgttta tttgaaacac accgagcttc tcatacatct aattatgtgc
    24841 ggcaagcaga agcgataggt ctagttcctt ttggacaaac caatgcgcca gagtttggtt
    24901 tcaaaaatat tacagaccca gtcatctatg gcccagcaag aaacccttgg aaccttgaat
    24961 gtacaccggg cggatcaagt ggaggagctg ctgctgcagt ttcatcagga atcgttcctt
    25021 tagctggtgc tagtgatggc ggtggttcta ttagaatacc cgcttcgttt tgcggattga
    25081 ttgggctgaa accttcgcga gggacgatgc cagttggtcc atatgcttgg agaggatggc
    25141 aaggagctgc tattgatttc ggcttgacag tttctatgag agacacggag gctttatttt
    25201 atgggatgag aagtatccat tccggtgcgc catatcaagt atcgccagtt gagtggcaaa
    25261 cacatccaag aaaaaaacgg ttgaagatag ctttttgtgt tgattcaccg atccactcac
    25321 agattagtga agaggcagta cttgctgtaa aaaaagctgt agatttcctt ggaaaagaag
    25381 ggcatgattt gacagagatt ccttacccac tagatggtcg taaactgatc acgtcttatt
    25441 atcagatgaa tggcgcagaa acagcagcaa tgatccgctc gattgaaaca ggcatccagc
    25501 gagctgtcag aaaagaagaa cttgaaccca tttcttggac tttgttccaa tatggtcaga
    25561 agctctctgc cgctacttac atcgattctc tgcatgtatg ggatgaagca gcaattgtca
    25621 tggaaaacct acatcaagaa tacgatctat tcctttcacc aacgactgca tttaccgccc
    25681 ctaaaatcag cgaagatttg caaagtgatg cgataagagc acagatggaa cacatcagtg
    25741 attgttctga agtagaaggt ttagacgttg tttcttccat gtttgaggag agcttgaaga
    25801 tcacacctta tacacagttg gctaatctga ctggacaacc agcaatcagc ttgcctactc
    25861 atttgacaaa agaaaacatg cctctaggaa tccaatttat ggctgccaga ggaagagaag
    25921 atttgctatt tcaaatcggc tatctatttg aagcaaatca tcagttccat ttaccaaatc
    25981 catggaaata gcagaaacaa ggagaagaag aatgaagaaa aaaagattgt ttgctctaat
    26041 aactagtgcc ttttttattt taagcgcttg cggtacagct gccgctaatc aggaagataa
    26101 cagctggagt aatgtagaaa aaaagaaaga attagtagta gcgacttctg gcacactgtt
    26161 tcccgcatct tattataatg atcaaaatga attagttggt tatgatgtag atttggcaaa
    26221 agaggtggct aaacggttaa atgtcaaaat ctcctttaaa gaatacaacg tggatggaca
    26281 aatcacttct gtttcaaaaa gagaatcaga ttttgctgcg aacgattttg gtatcacaaa
    26341 agaaagagaa aaaaaattct ccctttcaga gactttcaaa tattcatttg acagtatgat
    26401 cgtacgaaaa aaagatgatt caggaatcca ctcacttgaa gatcttaaag ggaaaaaagc
    26461 agctggcgaa cccaatacta gctatatgcg tttagcggaa aaacacggag ctaaattagt
    26521 cacatatgac aatgccacga atgatcagta tttaagcgat gtagcaaatg gtcgaacaga
    26581 tgttattcta aatgattatt atctgcagaa aatggccgta tctgctttac cagatatccc
    26641 tgtaaaaata ctggagaacg tttatttcaa tgaaaacgaa agtggttttt tattgaaaga
    26701 aggaaatgat gctctgaaaa agaaaatcga tggcgtatta aaagagatga aagaagatgg
    26761 gacgacaaag aaaatatctg aaaaatattt tcaaactgat gtttcagtca aaccgaaaga
    26821 aacgatggct gagtcggtga gtgagtgatg tactttcccc taattgattt ttccctcatg
    26881 tggacttcac ttcctttcgt actgcaaggc ttggtataca cattagggat tggcttcgtg
    26941 tcgtttcttt taggaaattt ggtagggttg cttttgacag ttttaggtct tttggattgg
    27001 ctgcctctga atgtatttat tcgtttttat ttatcgttct ttagaggcat tcccgcacta
    27061 gtcctgctgt ttctgttata tttcgggtta ccttatcaat taagtgcctt aacggcttca
    27121 gtcatctgct ttacgattac cagcagtgca tttattggtg aaatctatcg aggatcacta
    27181 gcaggcgttt cttcaggtca gtgggacgct ggtttagctt ctggtttttc tttctttggg
    27241 gtattgaaat atatcgtttt gccgcaagct tttcgaatct cagtaccggc actcagtaac
    27301 gtagctatgg atctagtgaa aggtacttcg ttagcagcaa caatcacagt gccagaaata
    27361 tttcaaaaag caaagatcat aggcggtcgg acattcgatt atttttctct ctatatttta
    27421 gttgcactga tttattgggt catttgtatc gggatcgaaa aagtgcaaca gtatttagaa
    27481 aaacgttcta tccatcagtc aatctgaaaa atagaaatgg tgctagcgtg gctggcacca
    27541 tttctatttt ttgtttcttg tatattctgc ctagtatttt aaaaccattt catgtaaaat
    27601 gtaaatcgaa aatatgttcg gaggtagaaa acatgaagac cccgctaacc atcgaaatag
    27661 aatcctcgtt ataccaatat tgcatcgagc aaggcggctt ggttgtagaa gaagtcacga
    27721 tgcctgatga ccaaggaatt gtagatacgc ttagctgctt gacaaaattt gatggaagcc
    27781 gagaatggcg atgctatgag ctgaaagtga caaaagcaga ttttcgctct acagccaaac
    27841 tgtcatttgt tggacattat aattattttg ttttaccaag taaactatat gaagaagtaa
    27901 aaactgaaat ccctacagga ataggtgttt tagtgtatcg tccgtacacg gttgaagagg
    27961 agatgcctgc ctctggtaca tttctgattg cgaaaaaagc agcaaagaaa gagcttgctg
    28021 taccggaaga agctttgaca aaccgcttca tggcttcttt attcagggaa gtaagaaaag
    28081 caaagcaaat ggagcgaggc gtaaaatatt ttccaaaaga tcaattgtac aaagaattga
    28141 aaagacgttc agaaaatcaa gatccatttt ctaacgaacg atattatgaa cggtttttag
    28201 aggaaatagc tagtcaggca atcaatgagt tgcaagaaga agttatcgcg cttcagcatg
    28261 attatgaata tttgaggcaa caacagcaga tccgtcggtt gcctacagag ccattggagt
    28321 gaaaaatatg tactatcctt atttaagagg aaaacaattt gacctattag cattaaaaga
    28381 agcactgtcg cgaggattgc tctccaataa aatccaacca gtgatcgaac cagtacgtga
    28441 ttccgctacg cttaaaaatg tgatcgaatt atttcaaaaa aagcaccatc caatcgctgt
    28501 gatccaaaat ccccaagtag gacaattcaa attattcgat cagcatgtgc acagttggac
    28561 agtcaaagaa aatagctctg tcgtccctgc acagatctta acgccagaaa atctcagtga
    28621 agtactggat tcacctccag cgtttcttgt tttcgatgga cagcactacc caaaagatac
    28681 agaggtgtgg aaacagttgg caggagttga ttcgaaattt ttgataccag atactagtcg
    28741 ctttcgcatc tggctgcccg aaaacaaaat tgtgatccgt gactcttttc aaacgagaaa
    28801 gcatgttgaa agttatgcag ataaaacaga tgactttttt agtgatgact atcttttttt
    28861 ccacgcagac ggatatattg gattttctga tttcacgatc gaaggtagcc gttatttcga
    28921 taaagggttc cctagccgag cgcttgctat ccatctaacg tatattgatg cttacggcaa
    28981 tatacgcgtg aaacattttg tctctgacag taacgactct gctaaagacc aagcattgaa
    29041 gtttcttgaa gcaggagata aaatgaaaaa atggatcatg cgtcaccatc atcaactctt
    29101 gattacttca ggaatgattg aattattggc tctttaccag caacaaaaat tccctggact
    29161 gggagtgttg aaaaagtggt cacttatgca tcatttggaa cttattagtc acctgctaga
    29221 tcatccaaaa gattggctta aatattattc aaaagttcag gaactatatg aagtgatcca
    29281 atgattagat aatgtaaatg aaaaaggaat agaaaagagg aaactggatg tatattcggc
    29341 aaacaaggaa agaagaagta gaagaagtgg tagaaatcat taatgccgca gcggatttca
    29401 ttgcttcgca aggctctcct caatggcaaa acgggcagaa accaacaaaa gaaacgattt
    29461 tgaacgatat tgaaagaaaa gaaagctatg tattaatagt ggaagatcaa attgctggaa
    29521 cagctgcatt agtctcaggc gtggatcccg tttacacaaa aatagatggt agttggaaag
    29581 aatcaactgg tccttattta tcgattcatc gtgtcgcttt agcagaaagt actcgaggaa
    29641 aaaaactagc aaaaccgttc ttagaacatt tgttgacgat tgcagccagc aaaaatatcc
    29701 aagatatccg tatcgatact tatccagcta atgaaccgat ggaaaagacc atctattctg
    29761 ctggattcac ttatcaaggg atgattgaat ttccttttga acacggcgaa agaaaagcat
    29821 atcaaataat catcaattga ctataaaatc atctgagcaa agataaagac attaacacta
    29881 ctgaaaaaaa caatcatatg atagccaaga ggttgtaaca gaagtggcca gcttcaaaaa
    29941 acaagaaaga atttccgaaa atgcttctca aatttttgtg gaattttagc gtattttacg
    30001 aaatagctgc tttctgaaac tcccttcatc gagaggtcgt gacatagctt ttgtcacgat
    30061 ctctcggctt atcctacgag tattattcga tacatttata tcaataatcc tttttttatt
    30121 ttatctgcag cttcttggaa cgctccatcg tttgacacga ctaaaagtga ggggatagct
    30181 ttcaaacgtt tcaactgtgc taaatcttct acataaaccg ccagtctggc tgaaatctgt
    30241 tcagatgaat ctccacgttt tttcaggcgc gattgcaatg tttctttaga gatcgtaata
    30301 aagataggac ggactaccgt cccgaaatac tcatataaat gccaaaaacc atttgctgtc
    30361 aaaacggcgt aacaatttcc tactgtcagt ttctttttaa tttctgcttt actggaacca
    30421 tatttatgtt gagcatattg gtcccactct gcaaactcat ttcccttgat catctgattg
    30481 aatgtattgt cagaaacaaa ataataatcc tcttgatctt tttccccagg tcttggtgat
    30541 cgattcgtgt aagtaatgat cttttgttcc ggctgaaaaa tagcttctgc gagtgaagtt
    30601 tttccagaac cagaaggacc gataaataca tagcaataat tcattgtcat ttctcctttg
    30661 atgattgaac aagtgaaaaa gctttggaaa atatttccaa agcttttaaa agtaactttg
    30721 cttttttgta ttttatacca gcaagccagt cacaatagca cttaaaaagc tgactaaagt
    30781 cgctgttagc aaaattttca atccgtgttt tgcgacaaga tttccttttt cttcatttaa
    30841 tcccttcatc gcaccagaaa tgatcccgat agaagagaag ttggcaaatg aaacgaggaa
    30901 gacagatata attgctgttg tacgctcagt aaaatggaaa ttaccatttg ctaattctgt
    30961 catagcaaca aattcatttg taaccagttt agtcgccatg atactaccgg catccactgc
    31021 ttctttccat gggattccgc tgatgaaggc aagaggggca aagatgtaac ctaggatctc
    31081 ttggaaagtg atgccgaata tcccgtggaa gatagcattg atcatggcaa tcaaagcaac
    31141 aaaaccgata agcatagcag caaccgtgat cgccacatgg aaaccatcaa gaatgtattc
    31201 tcccaacatt tggaaaaatg tctgttcttt gttttcttca atgatcagct catcttcttt
    31261 ttcttctaat acatagggat ttacaattga agccagaata taaccgccaa ataagttcaa
    31321 aacaagggca gtgatcacat atttagaatc gatcagtgcc atataagatc cgacgatcga
    31381 catagaaact gtagacatag ccgatacact catggtgaac aaacgtttct cagataggaa
    31441 gggtaattcc ttttttatgg aaataaaaac ttccgattgt cctaaaatag cagaagctac
    31501 tccattataa gattccaatt tgcccatccc gttgatttta cttaatccta gaccaacagc
    31561 tttcataaat aatggaagaa tcttgatata gcgtagaata ccgataatag cagaaataaa
    31621 tacaattggc atcaatacgg aaagaaagaa aggtgcactt cctttatttg ctacaccggc
    31681 aaaaacaaag tcaacacctt gcccagcaaa agcaagaaga tgatcaaaca tttttgccaa
    31741 tacagaaaca accgctgtac caaaagttgt acgtaataat acgaaaccaa gaacaagctg
    31801 taaaacgaac atcacaatta gtggtttcaa tttgattgct tttttgttac tgctgatcaa
    31861 aaaagcaagc cctaatgtga caaataaacc aataatacct aataaataac tcaaataatc
    31921 actcctattc caaattccgt tttattttac ctgttttgca aataaaatac taatctttag
    31981 tgaaattttc attacttttt tagagtgaaa cgcattcatt ttccattaga aaataaagcg
    32041 aacaaaataa aaggtttaaa aagtgtaacg tcttagtaaa gtctataaaa aaacgaaact
    32101 atcgattaag ctaatagttt cgttttcaac atttataagt aaactttttc tattggtaat
    32161 atagattttc ggaagggtag acaggtgcgc aggtaatatc gattcctagt tttttcaatg
    32221 tttgttcatc atttttattg accatcacag tagagtgggc ttgtgctccc tcaagttcag
    32281 ctagtttatc ataagcaact tgagcgattg gattcgttac tgcactgatt gataaagcga
    32341 tcaagacttc attgatgctt agtgcatcca aagaggaatg aagtgtcttt gatttcaatc
    32401 cttgtattgt ttcaagaact aatggtgaca ataaaggaat gtcatctgcg atatgggcta
    32461 aatgttttac tgcatttaag ataactgccg ctgacgcatc catcaatgaa cttgtacgcc
    32521 cggtaatgat gacttcatct ggtaattcga ttgcaatcac agcagaagga tcttttgtag
    32581 gactttgcgc ttgaatctta tctgcatagt catgggcagg gactaccacg tttcggtctt
    32641 caggacgtaa accgacttct tccataatca gtttgattcg attaactgct tcttcgtcga
    32701 tcaatccttt tttgaagtcg cattctgttt ggaaataacg acggatgatt tcttgtttag
    32761 aggcttcttg gacaacggta tcatccacga tcccaaatcc tacacgattc acacccatgt
    32821 cagtaggaga ttgataaaca gattctttac cagtaatttt ttcaataata cgtttgatta
    32881 caggaaatgt ctcaacatcc cgattatagt tcacagctac tttattgtag gcatcaaagt
    32941 ggaaggaatc gatcatattg acatctttta gatcgactgt ggctgcttca taagcaatgt
    33001 tcagcggatg tttcaacgga acattccata ctgggaaagt ttcgaacttg gagtaaccag
    33061 ctgcatgtcc tttttggctt tcatgataca gttggttcaa gcaagtagcg agctttccac
    33121 ttcctgggcc aggagctgtc actacaacga ttggtttagt tgtttcaata tactcatttt
    33181 ttccaaaacc atcttctgag acgatttttt ctacgttgac tggatagtct tcgatttctg
    33241 agtgtttata gaccttgatg tttcgtcttt ctagtttatt gataaagact ttcgtggcag
    33301 gctgtccact atagcgagtg ataacgacac tgttgattaa taagccataa ccacgtaatt
    33361 catcgattaa ccgcaagata tccatatcat aagtaatccc ataatcacca cggatcttat
    33421 tacgttcaat atctccagca tagacgcaaa tcagtatttc agcttgatct ttcaattttt
    33481 gcagcaattt gattttcgca tcttcttcaa atcctggaag tacacgtttc gcatgcttgt
    33541 cgtcaactaa cttcccacca aattctaaat ataatttgtc atagtggttc actcgttcca
    33601 aaatatacgc tgactgctct tcaatatatt tttctgaatc aaaaccaatt tttttcacta
    33661 ggttccctcc ttaagtacta aagcattttt tcattttttt gaggattcgt caactggaag
    33721 agcgaaattc ctcaaaaaaa caaagaaaat ggtttaaacc aacttgtcta gatagttatt
    33781 cattggtatg atagtttttg ataaggaagg tgctacatga aaaaaagaca gaaaaagaaa
    33841 aacgcatata aacactatat ccgttcgatt tttaccggtt atgaaagaat gcttgaagat
    33901 cccgaattgg aacaattgac gtttacttat ctaaacgaag aaacgcagct aactcgtgat
    33961 gagcatcagc gcattcattt cacaacaaga gatctgccta gtaaataaaa aataagataa
    34021 aggtcatcgg attcgattct gtcctatcag atggaaagac tagttgatcg aaaaaaaggc
    34081 tgtaccaaat tttggtgcag cctgttttat ggagcattgc ccgctctttt ttataacaat
    34141 tttttacaga aaatggtact attgaagtat taataagtga aaaacagatg agtaagagga
    34201 ggcaagtagt atgaaagagc cagatacaaa tgcaaattat gagccagacg aactagagga
    34261 gacgaacgcg gatcaggata caggacaaga atcgtcttct ttttacgata tcaaagaaaa
    34321 agcaagcatg aatctttccc aattattaga agacttacag gcttttgaaa aagctgtccg
    34381 cgaagaagat atccctgaga tctaccgttt atacaatggc cgattgaatg aagaattaaa
    34441 aagaagctca aatgaaaatc acgagatcga tcaattgctg atgcgtaaga tccatgatcg
    34501 ttttttacag gaatttcctt ttatggaaca agtagaaaac atctcgccaa cgatgagcta
    34561 ttacaaaatc ggcacttatt accatgatcg gcccacggtc ggtattgatg caagtctgcc
    34621 agaaattttt gttttgccta agatcgatga agagtgggag aaatattccc aaccggagtc
    34681 aacggactat gacaaaaaaa tcaatgagct agacgcaaag gtgatcactg ctcagacaga
    34741 aattgaacgt ttagatgaac aaataaaaga aatcaatcga caaatgactg atttgaacga
    34801 caataaagga ttcttgaatc gtaaaaaaat cgaggaagaa attcaagaat tagagaagaa
    34861 gaagcaagta ctatctaatg aaaaattagg ctggcttcct tatattgaga cgcctgaaac
    34921 aatccagcag caaaaagaag cattgaagca agaagcaaga gcagaccagc ttcgtgcagc
    34981 catcgtagaa aaagaacaaa ggcaaatcag acgctatttc ggtagcaaag agggattcgg
    35041 tcaagcaatc catgaattct tgatgaatta tcttggcaat gagaatccta ataatcaatc
    35101 taatgaagga ggttccgagt atgaatgaac acgatgaagc agaatggaca aaaaatgaag
    35161 aacaaacaga agatcatgaa gaaacgaaca cttcattgga agaacaaaca agtaatcctc
    35221 tggatgacac agaagaaatc cctatgctag aggaaacaaa aatacatgaa aaagaaaaag
    35281 taactccgac gaatacgaat actgcagaaa acacccatgc ttacgaaata actgaaaatc
    35341 ccgggtattt agaaggaaaa gagcaagatc atcaagatat cgtcagtttg attgaaacag
    35401 cggaagcaga attagcggat ttacgtatga gaaaagattt gatcgaatca gattttccac
    35461 aattattaga atcatatgat cgttttatat tgaatgaaga accaatctcc acgatcggaa
    35521 agacaagcct gatcgaatac ttcacgtatg aattactgaa accgttgaat gatattggtc
    35581 tggaacggtc aaattcagct tatgacacat ggaaaacaag acatttttcg atcaattata
    35641 cgttgaatgg acaaaacgaa ttagtctttt cctttgtatt accgaccatc gatcaacgat
    35701 accaagtgga atcgatgagt ctcctaaacg tatcaccaga gacaatggaa atcaatgtcc
    35761 aagatgaccg ggtgctttct ttgatccgac actggtcagt ggatcgagta ttctctgcag
    35821 ggcagatcac gatattcaat cacaaattga atcaattatt agcccatgca agaacacttg
    35881 gttttaccgt taatcagaca ttactagaca atacgaagcc acttcgtttg aatcttcaga
    35941 gcgaatttga attgacgaat gaagtattgg atgatatttt tatcgtggca atgaaaaatc
    36001 catcctacga tatggaaaag atcaacgaaa ctacttatca agtacgatta gataaaggac
    36061 agtctttaac gataacaaca aacgaaaaag atcaaacagt cttgtcgatc tcatctggag
    36121 actacccacg atctgtcatt gatttcttta tcaattacga attcttggtc ccattaatgg
    36181 tgagaaagtc ctgattggat aagttggatt accagatgtc attcagataa ttctgttcca
    36241 aaggacttgc ggcagttatc cagaataatt gtcacaagtc ttttctgtat gtactaaatg
    36301 gacgtgaata acttgctgat gaaggaggga tttcattagg aatgtccaca aataaaatag
    36361 cacagcttag cttattgtct gctgcttgtg tagcaggtag gatcgctttt caatttattc
    36421 cgaatttcca gccaatgact gctatttttt tatttatagt actttactta agcttaaaag
    36481 atgcgttaat tgtcatgagt ctttctattg cgatcagcag tttttatttt ggtgttggac
    36541 catgggtcat cggccaatgg atcagctata ctgttgttct gagtttgttt tcaatcgtct
    36601 gcttgagaaa aacagtgcaa aggaatctat ggttaaaggg agtctgtttt tttctggcgg
    36661 gcatggttta cggaataggt atgaccgttt ttgatacgct gttatatcgt ttgcctcaac
    36721 cttggatcta ttaccttcaa ggggtgtcgt ttgatttgat gcacagtata ggcaatgttg
    36781 tgttctttct tgtttttctg ccagtcgtca aacgttttta caatcattca ggaggaaaaa
    36841 atgaaaaaaa taatttatag tttgcttgca atgagtgtca gtatactcat tttgacaagt
    36901 tgcagtacaa cctatacaca atcggcttcc aaccaaaagg aggaagtaaa aacatcgcaa
    36961 aaagcaacaa tcacgttaca agaaaatggt caaaactttt cggaaaaaga agtggtcttc
    37021 aaaaaaggcg atgatttact gagtattttg aaacgaaatt ttgaagtcaa agaagataac
    37081 ggcttcatca cttcgcttga agggcactca caggatgaga aaaatcaact ctattggatg
    37141 tttactgtgg acgggaaaag tgctactact ggtgccaaag agatcaaatt gaatgatgga
    37201 caatcggtca ttttcaattt gtctaaacta taagtttcat aaaaaactgt taaagggcga
    37261 atattgccat aattcagtga gttcagttga tgaaagaagg aaaataacat ggaaaatttt
    37321 aaaaaaagcg gaatgttttc cgtaattgct gctctagggg ctaatattct agttgctatc
    37381 agcaaatttg tcggctatgc aatcagtggc agtgccgcca tgctaaacga aagcatccac
    37441 agtatcgttg attgcagcaa tcagattttg ctgttattcg gtgataaaca agccaataaa
    37501 ggacagagcg agttgcatca atttggtgaa ggacgagcaa aatacttttt cagtacaatc
    37561 gtcgcaatga tgcttttctt cggcggtggg gcacttgggg tgatggaagc ttttgaaaag
    37621 ttgacgcatc catcccatga agtgggtaat ccgatcattg ttatagcaat actgttattt
    37681 ggtttggtaa tcgaaggaag ttcgttacgc gtggcaatga aagaaatcaa agaattgaat
    37741 actgaaaaac tttccacgat gcgctttttg cgcgaaagtc gacacagtga aatactgatt
    37801 atttttacgg aagacttgtg tgctgtaatc ggtttggtct tggctttagc aggaacaata
    37861 ttgacttatg caaccgggat tgctgcattt gatgcgatca gcggattgct gatcggtttc
    37921 ttgctgatgg gagccgccat tttcctagca aaagaatttt atagtctgat catcggtgaa
    37981 agcgtgacag caagcgattt atcaaaaatc aagttagctt ttgaacgtcc tgagatcagt
    38041 cggctgatcg atataaaaac agttcattta agtccaacgg agattttagt tgcagcaaaa
    38101 gcagatatcg ttggtaacaa agagaatgaa gcttactcgg tcatcaatga tattgaaaaa
    38161 aatatgcgtc agtctcttcc tgacaagaag atgtatatat acattgaaac agataaatat
    38221 gacccaaatt attctcgtaa ataaaacgtt tattcatgaa ttgaagcaaa aatgagaaaa
    38281 atataatctt gtttttaaat aaaacggcta caatttagcg aatttatgtt acaatgagtc
    38341 cgctgtttat ttgttgtaat taaaggagat attttaatga acaaaaagaa aagtgcgcca
    38401 tggcttcaag ctttgcatgc agtgatgcca ctttgtataa gctatatacc agtaggatta
    38461 gcttgcggag tattgttgca aaaagttgga tttgatcctc tcttatcagg actgttatcg
    38521 attctggtct tttccggtgg cgcgcagttt ttagttgcat ctatgcttac gacgcaagca
    38581 tcatttgcaa cgacattatt gatggttttc tttttagaat tacgctatac attattagga
    38641 gcaagtcttg ccggttttat gaaaaaagaa aagcggacgt ttttggctgt cttttctcaa
    38701 tcactgaatg atgaaaacta tgcggtgaac tacttaaagt tttcaactga cccttcctgg
    38761 aataaaagaa aggctttata tgtcaattgg ttctcaatga ctgcatgggc cggtagcaat
    38821 atgatcggta attttttcgg ctcggtgatt cgagtagatg ctgaccttgt ccattttgct
    38881 ttgacggcca tgttcatttt catgtttatc atgcagatga aaagcgcact actgatcttt
    38941 acaggtttat tctcaggcgt attaggggtc gtattcatgg tattattcca aaatactttc
    39001 ggattgattg cagcaacagt cattgcttca tttgccggat ttatgctgga gaaggcattc
    39061 aaaaaagaaa aagctaagaa actaagagac cgcggtaaag atgtcaatga gccgctcttc
    39121 aacttcccgg aacaggaggc caatcaccat gactaaacta tatttgctac ttttagttgc
    39181 ttgtctgttc atcgtggcat atatcccgcg cttgttcccg atgctttatt ttactcatcg
    39241 aaaagtgcca tcctggttca gtgactggat gaaatatgta ccggtggcat tatttgcggc
    39301 gttagctttt aaagatgttt tcattactca tgaacatctg gatattgctt ggaatatcaa
    39361 aatagctgca atgattctag tagcaggcgt ggcttacaaa acacgttcga tggcgttgtc
    39421 agttttgaca ggacttgcgt ctgtcttttt gctgtctatg ctttaaactg ttttatttta
    39481 ttaaaaacag agattgcgac ataagttgtt caaactgatc tatccgaact gcatttgcaa
    39541 cagtcaattc tggatgcaag tttattcgta gggataaaaa agattagtgt tggcttatga
    39601 tgcaagctct tttttatttt aggaattaga gagggatttg tccttctatg attttttgct
    39661 ataataggga agatgtttat tttatgaaag aatagttgag ggaatttaaa tgaataaaaa
    39721 attgatgcaa ggaacatttt ggctaacctt tgctaatctt ctatgtaaag ttttaggggt
    39781 agtctatttg attccctggc taagtatgat ggggaacaat caagatggta tgcttgctac
    39841 gacattgtat aatgttggct atcttcctta tggattgttc ctgatgcttg gtacagtcgg
    39901 atttccaaat gcgattgcaa aaaaagtagc tgtagccaca aaagaaggag ataacgctgc
    39961 ttgtcggctg atttttagaa gtacgatcaa tatcatgttt gtcatcggta tcgcctctgc
    40021 tgcactgatg tatttatttg ctcctttact agccggaatc agtccgattg ctaacgttaa
    40081 taatgggata ctagctatca gaagtttatg cccatctttg attgctatcc caattcttag
    40141 tgcaatgaga ggatatttcc aaggaaaaaa tgatctgcgg ccttatggaa cttctttgat
    40201 tatcgaacag gctgtgcgag tgatcgttat cttggctggt acttattact tgcgcgtatt
    40261 gactgacgga accatattag aagctgtatt gatcagtaca gtcgcttctt tcttcggtgg
    40321 gttagctgcg attatccata tgtatatcgt cggacgcaga aaagacttct tcgaacttaa
    40381 agattttttg atttccagcc gttatttcga aagagaaaac cgatctgcct ctgtctctat
    40441 cattcgggaa acactaccgt ttatttttgt cggctcagtc attactatcg ttcaaatgat
    40501 cgaccaagta acgatgaaac cactgcttca ctttttccgt ccagagatcg cagatcagca
    40561 actggaattc ttattcagcc gtgcttctgt caatccaaat aaattgacat tgatcctgat
    40621 ctccatggta ggaacagtag ctataagcag cttgccaata ttaagcacga tgaagaaaaa
    40681 agatcggata caaatcgaaa aaacagtagg agatagtttt tcgattgcgt tattgattct
    40741 tcttccttca ttgactggaa tgtctttatt agctggacca ctatacacat tgttttttgg
    40801 ctatgatcct gaaagtgtcg gatatttcca aatggctttg ctagcttctt tgtttttctc
    40861 tttattcacg atcctatcta cgatgcttca atctttgaat caccatcgtt ttgcgatccg
    40921 attaactgta gaagcaatta tcctgaaagt catcttccaa gtaatcggat taggattagt
    40981 tggcggatac gggatgagct tatcaaatgg cttagcattc ggtattgtct ttgttcgcgg
    41041 ctatcacttt atgtgcaaag aataccgaat agcaccttta gcaaagatca gcaatttttt
    41101 cttaaaaact ttccgctcca ccctgattat gcttgcagta tgttttgccg ttttcttttt
    41161 gctgaatcag cgattgtcaa tggaatcaaa aagctatgca atgatttatt gtgtggctgt
    41221 aggaagtatt ggcggcttag tatttttatt ttctcaattc ggaaaaaatg ggtttcgaat
    41281 gttgaaaaat gttaaacatc atagcagaag caaagaatag gttaactaat cgttttttgc
    41341 tgtcacttga aaaacggcaa ctcgtatgat tacgcttgtc gtttttctat tttccaattt
    41401 tagtttcata tctaatctta cttatgatat agttataagg tctggaaaaa ttctttgaaa
    41461 cgaggaaatt aaacacatga aacttgcagt agttacagat agttcggcat atctgccgga
    41521 acgtatcaag aaccatcccg acttattcgt tattccaatc cctttgatta tggatgggaa
    41581 aatttataac gaaggaatcg atatcgaagc ggatgaatat tatggacttt taaaaaacag
    41641 taaagatttt cctactactt cacaacctgt cgtaggggaa gttttgggtt tatatgagga
    41701 attgaaagag aaagggtatg acacgatttt aagtatccat ctttcttcag ggatttctgg
    41761 ctttgtcaac acattatttg cgatgaaaga tgacatcagc ggagtaacgg tcattcctta
    41821 tgactctaag atcactagta tgccgatggg acacatggta gaagctgcct tagacttaaa
    41881 tgatgaaggt cagtcccttg atgaaatact tgcacatatc gatcgaatcc gagataatac
    41941 ctatgcttat ctgattgtag atgatttgaa caatttagta cgaggaggac gtttgacaaa
    42001 tggtgcagcg ctgattggca gcttattaaa aatcaaaccg atcctgacat tttctgaagg
    42061 gaaaattgtt ttattcgaaa aaatccgttc aagtaaaaaa gcatttgctc gagctgaaga
    42121 aattattggt agaagaaata acgaaataca accgccagtc aaactttacg tgatacatgc
    42181 aaataattta gaagtagcga ttgcggagaa agagaaattg cagaaaaaat atccaaatgc
    42241 agaaatcgaa attggtcatt tcggaccagt cattggtaca catttaggtg aaaaagccat
    42301 cggcattgcc atttcagcgc aataattcct tttattcaaa aaacatacca aaaatat
//
LOCUS       DAFCNS010000019        38600 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFCNS010000019 DAFCNS010000000
VERSION     DAFCNS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.019"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1229)
                     /locus_tag="LNE23_000949"
     CDS             complement(99..1229)
                     /locus_tag="LNE23_000949"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBK6930822.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="LNE23_000950"
     CDS             complement(1878..3080)
                     /locus_tag="LNE23_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6930823.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="LNE23_000951"
     CDS             complement(3140..3343)
                     /locus_tag="LNE23_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBK6930824.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="LNE23_000952"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="LNE23_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="LNE23_000953"
     CDS             4880..5692
                     /locus_tag="LNE23_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930825.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="LNE23_000954"
     CDS             complement(6716..6868)
                     /locus_tag="LNE23_000954"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK6930826.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="LNE23_000955"
     CDS             complement(7059..7937)
                     /locus_tag="LNE23_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6930827.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="LNE23_000956"
     CDS             complement(7976..9094)
                     /locus_tag="LNE23_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBK6930828.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="LNE23_000957"
     CDS             complement(9091..11190)
                     /locus_tag="LNE23_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBK6930829.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="LNE23_000958"
     CDS             complement(11241..12062)
                     /locus_tag="LNE23_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6930830.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="LNE23_000959"
     CDS             complement(12059..12868)
                     /locus_tag="LNE23_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6930831.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="LNE23_000960"
     CDS             complement(12889..13383)
                     /locus_tag="LNE23_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6930832.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="LNE23_000961"
     CDS             complement(13395..13883)
                     /locus_tag="LNE23_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6930833.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="LNE23_000962"
     CDS             complement(13960..16656)
                     /locus_tag="LNE23_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6930834.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="LNE23_000963"
     CDS             complement(17084..18157)
                     /locus_tag="LNE23_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6930835.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="LNE23_000964"
     CDS             complement(18154..18981)
                     /locus_tag="LNE23_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6930836.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="LNE23_000965"
     CDS             complement(18982..19788)
                     /locus_tag="LNE23_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6930837.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="LNE23_000966"
     CDS             complement(19800..20885)
                     /locus_tag="LNE23_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6930838.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="LNE23_000967"
     CDS             complement(20902..21444)
                     /locus_tag="LNE23_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6930839.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="LNE23_000968"
     CDS             21702..22691
                     /locus_tag="LNE23_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBK6930840.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="LNE23_000969"
     CDS             22688..23425
                     /locus_tag="LNE23_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBK6930841.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="LNE23_000970"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="LNE23_000970"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBK6930842.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="LNE23_000971"
     CDS             23989..24621
                     /locus_tag="LNE23_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6930843.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="LNE23_000972"
     CDS             24706..25353
                     /locus_tag="LNE23_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6930844.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="LNE23_000973"
     CDS             25358..26341
                     /locus_tag="LNE23_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBK6930845.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="LNE23_000974"
     CDS             complement(26744..28258)
                     /locus_tag="LNE23_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBK6930846.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="LNE23_000975"
     CDS             28396..29202
                     /locus_tag="LNE23_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930847.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="LNE23_000976"
     CDS             complement(29377..29946)
                     /locus_tag="LNE23_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6930848.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="LNE23_000977"
     CDS             complement(30029..32089)
                     /locus_tag="LNE23_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBK6930849.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32366..33844)
                     /locus_tag="LNE23_000978"
     CDS             complement(32366..33844)
                     /locus_tag="LNE23_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930850.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34142..35077)
                     /gene="murB"
                     /locus_tag="LNE23_000979"
     CDS             complement(34142..35077)
                     /gene="murB"
                     /locus_tag="LNE23_000979"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBK6930851.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35150..36070)
                     /locus_tag="LNE23_000980"
     CDS             complement(35150..36070)
                     /locus_tag="LNE23_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6930852.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            36183..36935
                     /gene="xth"
                     /locus_tag="LNE23_000981"
     CDS             36183..36935
                     /gene="xth"
                     /locus_tag="LNE23_000981"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBK6930853.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            37799..38332
                     /locus_tag="LNE23_000982"
     CDS             37799..38332
                     /locus_tag="LNE23_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBK6930854.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT        11576 a         7961 c         6543 g        12520 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctatcg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatca aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgtttca
    32221 agaaaatcgg aataataact tatgtacttt ttaattttgt taagtaatat atctagttat
    32281 atctagttat atctagttac cttttctttg aaaaaaacat aaatcagcta ataaatatgt
    32341 gagacaaagc tgccatccat aaatactagc tgattttttc agttaaatgt ctattttttt
    32401 ctctgatgat ggtatttcca aaatattccc agtcatctgg agtcggtgga tttttccaaa
    32461 ttatctgttc tttattaaat ttttcaagta aacaatcaga taaataaata ttcgtacgag
    32521 aagtgattcg actctcaatc acaatattgt aatttttaaa tcctttcact tgagaaataa
    32581 tgtaatcggt ataagcagat ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa
    32641 aatatgatac cggacaaata ccgaccaata aaaagagata atcataaaat aagcgaatca
    32701 tgaaacgttc atcttttccc aagaaatagc gtttattctc ttgtatgacc ttttggattg
    32761 ctgtactgaa tattggatac gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc
    32821 tagtcatctc aaagtcgaat atccaatttt ttcgattgac atgcatcata cccatacgaa
    32881 gttcttttcg tatatatagt ctttcttctt cagagatttc aatcgaaaat ggtaattgat
    32941 caatcaattc cactgacatt ttttgactaa tcatgtggat atcatgaaaa tgagcttccg
    33001 caagtccaaa tgcgccatct aattcaaatt cttcttccgt gatcaaaaac aacatgatat
    33061 aagaaatctc atcaatgatc tgctctttag ggatatgaag aaaagaaata tgtgataaat
    33121 ccttgatgat cttcattcct tctgagctct taaagtattc atctgaaaag aaacgttttc
    33181 cgattggttg attcccatag gccctcaaca aactaatagt ggttagcaaa ttgaatttga
    33241 ttcgttttgt tgcagacagt tttaaattga acagatattc aaaatattgc acgatactct
    33301 ttgcttcatt catgacgctt tctctaaatg gcatagaaaa accattaaag accccgaaat
    33361 ataccccaaa taatacgttc cgtatgttta tttcttcccc aactaatatg tttttcttta
    33421 actttatttg atgctgttca agaaaagaga ctaattctct tctttttaca taggctcttg
    33481 atatgcttaa aaaatgattc tctgcaaatt tttcgatcgt tacttttgaa tccacgatct
    33541 ccgttaacag tcgagcaatt tttgatcgtt gaaagtagat tcttcttaaa tttttgatat
    33601 ccgtcaaatt cagacgttcg acatggatct ctgcttcttc tgaaatttgg atctctggct
    33661 ttgttttgaa ttcagtaaat tctttattta attctatgat ataatttctt gttttaaatt
    33721 tagacaaacc taatgtttca tataacttag gcagcactac ataattcttt ttctcaaatt
    33781 ctaaatatct agctagcagc agtttttctc tatcttcctc ttctataagg taatccaact
    33841 tcatatccta cacctcaaaa aatgcctcat accaattact tgatatgcag atgaaagttc
    33901 atcttttcac ctatactgat tttatgatgg attatgcttg ttttttcatc agttattctc
    33961 attttttatc ttttttttga gaatattgca aaaaatggaa aaatttatga ggaaatattc
    34021 tatatttttt gttttattgt gttaaaatgt acaagatttg cgccatttgc gtaaacaacc
    34081 acaaaaaaga gcaggacggc caccgtcctg ctcttttact tattttaagt tccattactt
    34141 attaattggc taaacttctg cttgtttctg atattgcttg tttttctcca atgatccgca
    34201 cttctgtttc taaagacaca tcaaattttt ctttgattac ttcttggatg tgcgcaatca
    34261 attcaatata atctgtcgct gtcgcatgat cgatattgac gatgaatcca gcatgttttt
    34321 cagagacttg tgcaccgccc catttcaggc cttgcaatcc tgcttgttgg atcagctgac
    34381 ctgtatagtg tcctactggg cgcttaaaga cacttccgca tgaagggtat tctaatggct
    34441 gttttgattg acgcaattcg gtcaactcat ccattcttgc tttgattgct tcataatccc
    34501 ctgtttgcaa actgaatcga gcttccaata cgatagcacg taattcttgg actttactgt
    34561 gacgataaga aaataccatt tcgtcttttg tcaaagtttt caatgttcca tcttcaagca
    34621 aaagatccac ttcagcaaaa acatctttga tctctccgcc atacgcacct gcattcatat
    34681 aaaccgctcc accgacactt cccgggatgc cgcaagcaaa ttcaaatcca gtcaatgatt
    34741 ctgccaaagc tgcataagtc gtatcaatca gtgaggcacc tgcttccgca atgaccatcg
    34801 ttcctttgac atgaatctgt ttcatttcag tcagcatgat gactacaccg cgaatcccgc
    34861 catcttgaac gatcagattg cttgcatttc ctaataccat ccaaggtata tcatggattc
    34921 ggcaataatc gatgatttgc tttacttctt cttgtttttt cgggaatgca aggacatcag
    34981 ctggtccacc tgttttcgta aacgtatagt tcatcaatgg ttcatcaaac aatatagtaa
    35041 tcgtggctaa gctttcaact atttcttctt tattcactga ggattttcct ttcttttctc
    35101 aaacgtcctc attattctat cacgcttaat acaatcttgc cagtcttgtt caaagttgat
    35161 gacggtagtg atctttaagc acttcctcac ataattcatg acttcttaca acttcttttt
    35221 gtttaagcaa ttctgtttcc gatttaccta aacggatctg agaaaagaag gcctctacca
    35281 ttggctcaaa cccgcgtttg actagcgtgt ttgtccagtc gccaaattct ttcacttttt
    35341 ttccatcagc atcttgaatg acaagctggg tcagatcgtt tacttcataa gtaccatttt
    35401 tacttgttac ttgatatttt tctatattag cgccacttgc taaatccatc gttaaaatag
    35461 ccgtttgatt ttgtgtttct aattgtaaaa tggcatattc caaaaattcc tttgtttctc
    35521 ttatctggta ttttgttctc aaaatgggtt catctaataa atacacagct gtatcaataa
    35581 gatgtaaaaa caaatcataa aggacgaact ctgtcgtttc ttttgccgct attcgatttt
    35641 tttgcaggtg gatcaaccgc ttatctggga tacgtttcaa ttcttctacc ataggagcaa
    35701 agcgccggtt gaaaccgatc atcaaaatta tttggttctt atcagctaat tcttgcaatt
    35761 cacgaacttc atttagatgg acactcagtg gtttgtcaat ataaacatgg ataccatttt
    35821 ctagacattt tttggctaat tcataatgta cttttgttgc tgcgtggatc atacatgctt
    35881 cgattttttc ttctaaaagc tcatcgatcg tttcatatag atgttggaac ccgtactctt
    35941 tttgaattct ttcttttgtt tctttatttc ttgttgcaaa ataaaaatca agtgttcctt
    36001 gttttttcgc ataagttggt aaatatgctt tttctgcgat attccctaca ccgattacac
    36061 caatcttcat ctactttcct cctggatcat ttagttactt tcatcttaca ccatttttta
    36121 ctaaaatgag gtacaatagt atcgcaaagg taaggaaatt aattcgaagg agtgtgcttt
    36181 tattgaaatt tatctcttgg aacgtaaacg ggcttcgtgc catcgtaaat aaaaattttt
    36241 tagaagtttt tcatgagtta gacgccgatt ttttctgtct gcaggaaacg aaactccaag
    36301 caggacaaat tgatttagat cttccaggtt attatcaata ttggaattac gcggaacgaa
    36361 aaggctattc cggcacagcg atttttgcta aaaaaccagc attgaatgcc acttacggaa
    36421 tgggtatcga tatccatgat acagaaggcc gtctgattac tttggaatat tccgatttct
    36481 ttcttgtaac ctgttacacg ccaaattctc aaagcgaatt aaaacgttta gattaccgat
    36541 tagaatggga agaagccttt tataattatt tggaaaatct aaaaaaacaa aaaccagtca
    36601 tcgtgtgtgg ggatctaaat gtcgctcatc aaaagattga tttgaaaaac tggaaaacga
    36661 atcaaaaaaa tgctggattc actccagaag aaagagcggc tctttctcga ctgttggata
    36721 acgggtttat cgatacattc cgttattttt atccaaccca ggaaggtgtc tactcttggt
    36781 ggaactaccg attcaattca aggaaaaaca atgctggttg gcggatcgat tactttttga
    36841 cgagcaaaga tttagagcca ggactagctg atgcaaagat ccatacggac attatgggca
    36901 gtgatcattg tccagtagaa ttagatttga aataaagagg ttgtgaaaaa gtcgtttagt
    36961 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac attttgcata aatttggcat
    37021 aataccgaga aactggcttt tgaacactgt ttatcgagag attgtgaaaa agctgtcctg
    37081 catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttgggc
    37141 ttattaccgc agatacaagc ttttgaacac cgtttattaa gaggttgtga aaaagctgca
    37201 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttt cgtttggttg
    37261 ggcttattac cgcagatgca agcttttgaa caccgtttat taagaggttg tgaaaaagct
    37321 gcactgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt tttcgtttgg
    37381 ttggacttat taccgcagat gcaagctttt gaacaccgtt tattgagaag ttgtgaaatc
    37441 agcgtcctga cctgagtatt agagaaacaa atggcaaaat agttcctcat attttgctat
    37501 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttagtggaga agttgtgaaa
    37561 aagtcgccta ctttcgagtc gtaagaaatt tttttgaaaa tggttcctca caactttgca
    37621 taaatttgac ttaataccga gaagtcatga tccccatcta actccaaatc acaaggaatg
    37681 agttcaaaaa ctagcttttc tcaacaaata ttacataaaa aattctatgt tttttgttaa
    37741 atttatttgc ttttttcaaa agtatgttac agtaatttcc caaggaggtt ttacagaaat
    37801 gaattttgtt gccatggatt ttgaaactgc caatcatcag ccttatagtg cttgttctct
    37861 tgcgctagtc atggtaaaaa atagccagat cgttgatgaa ttctatacat tgatacagcc
    37921 tgagactcct tttttctggc gaaatgtcca aattcacgga atccatcaag aagacgtacg
    37981 caatgcgccg aaattcccag aagtctggca aactatccaa ccatattttc aaaaaaatcg
    38041 tttagtcgtt gcacataatg ctgcttttga tacaaaggtc ttagcgggat gcttagatta
    38101 ttatcatctt gaacagccta attatctttc tttatgtaca gtgaaaagta gccgtcgcct
    38161 gtttcctgaa tttccaaatc atcgattgaa cactgtgtgc gaaaatttga acatccaatt
    38221 agatcatcac catgatgcat tggaagatag tcgtgcatgt gcggaaatcc tgctttatca
    38281 agaaaaaaaa ttcgggacag atccattgaa aaaacttgtg actataaagt aaaagaatac
    38341 ttatgaaaca cacaaagagg tcgtgagcct gccgtttcac tccgagtcac aaggagcaat
    38401 ttcaaaaaat agcttctcat gtttattaaa tattgtgact ggtgaccgag gagctggcag
    38461 gagaacaccg tttattggag aggttgtgaa aaagctgttc tgcatcaagt aataagaaca
    38521 gccaaacaaa aatagcttct catatttttc atttggttgg acttattacc gcagatgcaa
    38581 gcttttggac accgtttacc
//
LOCUS       DAFCNS010000020        38104 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFCNS010000020 DAFCNS010000000
VERSION     DAFCNS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.020"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(513..1457)
                     /locus_tag="LNE23_000983"
     CDS             complement(513..1457)
                     /locus_tag="LNE23_000983"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK6930855.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1753..2697)
                     /locus_tag="LNE23_000984"
     CDS             complement(1753..2697)
                     /locus_tag="LNE23_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930856.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2918..4132
                     /locus_tag="LNE23_000985"
     CDS             2918..4132
                     /locus_tag="LNE23_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6930857.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4154..6169
                     /locus_tag="LNE23_000986"
     CDS             4154..6169
                     /locus_tag="LNE23_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBK6930858.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6252..7625)
                     /locus_tag="LNE23_000987"
     CDS             complement(6252..7625)
                     /locus_tag="LNE23_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK6930859.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7651..7935)
                     /locus_tag="LNE23_000988"
     CDS             complement(7651..7935)
                     /locus_tag="LNE23_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6930860.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7937..9952)
                     /locus_tag="LNE23_000989"
     CDS             complement(7937..9952)
                     /locus_tag="LNE23_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6930861.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10131..10436)
                     /locus_tag="LNE23_000990"
     CDS             complement(10131..10436)
                     /locus_tag="LNE23_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBK6930862.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10525..11556)
                     /locus_tag="LNE23_000991"
     CDS             complement(10525..11556)
                     /locus_tag="LNE23_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6930863.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11763..12638
                     /locus_tag="LNE23_000992"
     CDS             11763..12638
                     /locus_tag="LNE23_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6930864.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12790..13632)
                     /locus_tag="LNE23_000993"
     CDS             complement(12790..13632)
                     /locus_tag="LNE23_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6930865.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13724..15601)
                     /locus_tag="LNE23_000994"
     CDS             complement(13724..15601)
                     /locus_tag="LNE23_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6930866.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15602..17020)
                     /locus_tag="LNE23_000995"
                     /pseudo
     CDS             complement(15602..17020)
                     /locus_tag="LNE23_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(17023..20412)
                     /locus_tag="LNE23_000996"
     CDS             complement(17023..20412)
                     /locus_tag="LNE23_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK6930867.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20669..22060
                     /locus_tag="LNE23_000997"
     CDS             20669..22060
                     /locus_tag="LNE23_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930868.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22133..22780)
                     /locus_tag="LNE23_000998"
     CDS             complement(22133..22780)
                     /locus_tag="LNE23_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK6930869.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22788..23474)
                     /locus_tag="LNE23_000999"
     CDS             complement(22788..23474)
                     /locus_tag="LNE23_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6930870.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23480..24553)
                     /locus_tag="LNE23_001000"
     CDS             complement(23480..24553)
                     /locus_tag="LNE23_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6930871.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24775..25449
                     /locus_tag="LNE23_001001"
     CDS             24775..25449
                     /locus_tag="LNE23_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6930872.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25454..26794
                     /locus_tag="LNE23_001002"
     CDS             25454..26794
                     /locus_tag="LNE23_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6930873.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26943..27929)
                     /locus_tag="LNE23_001003"
     CDS             complement(26943..27929)
                     /locus_tag="LNE23_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBK6930874.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28653..29684
                     /locus_tag="LNE23_001004"
     CDS             28653..29684
                     /locus_tag="LNE23_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6930875.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29793..31325
                     /locus_tag="LNE23_001005"
     CDS             29793..31325
                     /locus_tag="LNE23_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBK6930876.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31464..32276
                     /locus_tag="LNE23_001006"
     CDS             31464..32276
                     /locus_tag="LNE23_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBK6930877.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            32266..32751
                     /locus_tag="LNE23_001007"
     CDS             32266..32751
                     /locus_tag="LNE23_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6930878.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32774..33319
                     /locus_tag="LNE23_001008"
     CDS             32774..33319
                     /locus_tag="LNE23_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBK6930879.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33392..35035)
                     /locus_tag="LNE23_001009"
     CDS             complement(33392..35035)
                     /locus_tag="LNE23_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK6930880.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(35164..37392)
                     /locus_tag="LNE23_001010"
     CDS             complement(35164..37392)
                     /locus_tag="LNE23_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6930881.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37425..37904)
                     /locus_tag="LNE23_001011"
     CDS             complement(37425..37904)
                     /locus_tag="LNE23_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930882.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11122 a         7200 c         6926 g        12856 t
ORIGIN      
        1 tgaatagtag gaaatagtgg tttagaacta gtgggctgat tttttatata ttttagttta
       61 cataatatat attatacaaa gtagatttaa aaaatgataa acctctctcg aacggttttt
      121 tatcaaaaat cgtcattttt ccatcaaaat ctgcttatgc aaaattataa aaaaattttg
      181 cagtttttct tacttttttt gtcgtttaat tgaatgataa aaaccagttt ctttattcag
      241 ttaatcaaat atgaacgaat tagatatgaa tcattgtatt tgccgttgcg gaaggatttt
      301 ttaattaacc acagataaac attgataacg attcgcttat tgtaatcaac agattaaatt
      361 tcatatgttc atatgtacgg atacattttc ggaccgatta attttaacca ttctatttat
      421 ttgcttacct attagatatc aataaaaaac tgcttaacag ctatttcagc tgttaagcag
      481 ttttaggatt tttagttcat attcattgtt tcttataata cagtgttcaa tacttctctt
      541 aaagcacttg ttgatttgct gaacattgct ttttcttctt cgttgattga taattcaaca
      601 acttcacgaa caccattttc gtcaacgatt gatggcacac ctgtaaagat atctttttcg
      661 tcgtattcac cagttaaata agcagataca ggcaatacag cttgttcgtt attcaagata
      721 gcttttacga tacgtgcagt actcatacca ataccatagt aagttgcttt tttacgatca
      781 atgatctcat aagcagcatt acggacttta tcagcaatca catctaattc atcttgggca
      841 atacgatggt ctttttctac aatttcaaac actggtttac caccgacagt tgtatgtgac
      901 catgcagcaa cttctgaatc accatgttca cctaagatat agccgtggac gctgcgtggg
      961 tcaactttta atttcaatgc gatttcttta cggaaacgag ttgtatccaa tgttgttcca
     1021 gtaccgataa cacgtgatgt tggcagacca gattcgttcc aagcgatata tgtcaaaata
     1081 tctactgggt ttgaagcaac aacgataata ccgtcgaatc ctgatcccat gatttctttt
     1141 acgatttggc gcatgattga cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt
     1201 ttttggttga taccagctgt gataacaacg atatcagcat ctttacattc ttcatagcca
     1261 cctgaccata cagccacatt ttcatcgccc caagccatac catctaaaag gtccaatgct
     1321 tccccttctg ctttttcttg attcacatcg atcaatacta attcattaga gattccttgg
     1381 ttgatcattg cataagcgat acttgttcca acaaaacctg taccgacgat tactacttta
     1441 cgacttgttt ttttcatgaa taaacagctc cttttcataa ctatttgtta ctttcatgaa
     1501 aagtatagtc ttgtttattc attttatcaa catgttaatt ataatagttg ttattataat
     1561 ttttgatgga aacgaattcc tgaatacgca taacagataa atgacaaatg tttacaccta
     1621 tatcccctga taatacaacc aaaagtttag aactgaaaat aaatccaaaa aaattcgctg
     1681 caacgatata ataagacagc aatcgcacct aaaacaattt tccttcatat gctttagtga
     1741 aagattttct tcttaatgtt tatttctcca ttcattggga gtacatgttg aatacttctt
     1801 gaataatttg aataaagaag tcgtttgttt atatccgata cgattagcga tttcttccaa
     1861 cgaaaaactc gtttcttgaa tcagttccaa cgcaaagtcg aattttttct ttagaaccag
     1921 ttcttgaaag gaagcatcaa aatacatttt caatttatta ctaagataga ccggattgta
     1981 gccaaaatga gctgctgttt cttttaacgt acaagaagta aaatgcttat cgatataatc
     2041 taatactcta agcatctcat tttctgcgca tccatctgct gatgaaaagc tttgttgttc
     2101 taataaatga gtcaattcga tcagtaatag tgatgtatag agatttaacg aacggatctt
     2161 atgactgtat gtatcaaaat aaatcaacag catattttca atcaaatgcg tcaaacgact
     2221 tgtttttccg ccgcgaaaat gaatgaattg tgtatgttcc cctttttctt ttgatgcatt
     2281 tatcaaaaaa gaagaaagta gattatttgt gcttgccaaa tgattcaata tctctgtaga
     2341 taaggtttct tcttttaata agatgttgat taaaatatcc tcttgccctg tgtattcgat
     2401 tctttgagta gcttctttat caatgatcac cacgtcactt tcttccatct ctatcctttc
     2461 tccattaata atttgtaaac atgtaccgct atacatataa ttgatttcaa caaaatcatg
     2521 tgtatgctcg ggaacttccg catatcgacg gtgtttactg atatgaatat ttccctgttg
     2581 aaatagatcc cctcggtgga tttttggcgg tgtctgaaga tggtaagcta agtttaaatc
     2641 attgatattc attccttgtt cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa
     2701 aaatgtctaa ttcagctagt tccaattgac tccctccttt ttgtaagcgg tttctttcgc
     2761 ttattagttc acgctgtact gctattatat gcaaagctat ttttggtttc ataaagactt
     2821 tctatggttg ggacatgaac ttcctgtgat tccgctattg agagaataac cacttcatac
     2881 tatatttttg ataagcgctt acaaaaatga aaggaacatg cttatgaata aaaagatgat
     2941 gaaattttct ctgctgtcag tctctcttat tttgacttct gctggtgcag tcacgtctgc
     3001 aattcccagt atgtcagcta cttatgccac gcgttcttta tctgctgttg aattattaac
     3061 gactgctccc tcttttatgg tcactatttt tgttttgatt agtagtttta ttgctaggaa
     3121 aatcggacaa aaacaaactg tgatcatcgg attagtgatt gcatcgattt gtggtatcct
     3181 ccccctcttc tcacaaagtt acagtattgt cttagcttca agagctggac taggtattgg
     3241 ctttggattg atcaattctt tggcagtcag catgattgct gccttttatc acggagatga
     3301 acgagcctct atgattggtt ttcaaagtgc atttcaagga tttggtgcag ctttaatgac
     3361 atttgttgca ggacagctag tacagttcgg ttggcaatat acctttttag tctacgccat
     3421 cagcttacct attcttgtct tgtttgtttt gtttgttcct acacctgaaa caactgaact
     3481 tcaatcgcat ggcacagccc caacaagaca agtaatcaaa acgaatccta aaatgattag
     3541 ttatggtatt tttctcttgc tggtcatcgt ggtatataat gctgtatcga ttaaattagc
     3601 cacagtcctg acgacttatt ctctaggaaa cgaaactaat gcagcgaata tcttaacaat
     3661 catgcaattg gcaagtatgg ctgccggagt ttgttttggc tggcttcaga caaaagcaca
     3721 tcgctatatg cttacacttt ctttactact gatgggaatc ggctttgtat tgattgccac
     3781 tgtgataaac ctgtatgtga ttgggtttgg tgcgatccta acaggaattt ctttttcgtt
     3841 attcattcct tacttattca accaagtaag tatccaagcg cccaagtacg cggagagttt
     3901 caatacttca atcttattgg ttggcgctaa tttaggtact ttcattgcac catatggttt
     3961 gctctttctc tcttttgctt ctcttgggag taaaacactt gctgttttcg tcaatggcag
     4021 tatcttgctg ttgattagcg cgcttattag ccttctagta gtacacagga cgaatagaaa
     4081 gacagtgata agagtagatg aagaaataaa agaacaattg acaacaaaat aaatttgatg
     4141 gaggtcgtgt aaaatgacaa atgaaaaaca taaagataga tggctttggt atcctggaga
     4201 ctttgagatc cgccatggac tgctacaaaa tttccaacgg gaagaacgag gttttgactg
     4261 gccagcgtac tggtacatgg atgattgcca tcggaacgtc aagtttaaga gatattactt
     4321 tcttgatcaa ccttccatgt ttaaagtgac gatccaagga gttgggtatg tagaaattaa
     4381 tggacaaaaa catccatgtg gaaaatggct gacttgtcca gctggtaaag caaaaattcg
     4441 tatctttgta ggacatacat cagggctacc agcgatgttt atcgagggag acgaagtaaa
     4501 atcagatatt ggctggacag caagtaattt tattgaagaa tatccagctg gctggagtcc
     4561 gctttatgtc gatatcgcaa aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca
     4621 tccagtcact gaaacggaga ttggcggagg aatcttattc gattttgggc gagcagtgaa
     4681 tggctgtgta gaactaatat ctctggatga ccatatcgaa tctatcactc tatgttatgg
     4741 agagtccgat gtagaagcta tggatatcga ttattgctac tataagcaaa taggaatgac
     4801 aagaaaaatg aaagctcgta aacgcgcttt tcgctatttg tttataccag aggtttctac
     4861 tggaatggtt caacttcaag tttttcatga atttttatct ctttcgaaac aagctgcttt
     4921 ttcaagcgat tcttctttac tgaatcagat ctggtcagta tcagaagaag ccttcaagtt
     4981 atgtagtgat ttgtttttta tcgacggtat caaacgagat cgctggattt ggtctggaga
     5041 tgcgtatcaa tcattcatga tcaatcaata tctttttttt gacgaggaaa tcaataaacg
     5101 aacgatatta gcattaagag gacaaaatga aatcaagcag catatgaata ctatcgttga
     5161 ctattcgatc ttgtggttga ttggtatcga aaaccactat atgatgacga aagactatga
     5221 atttttacgc atcgtctacc ctaagatgca atcattaatg agatacttga tggagcaaac
     5281 gaatgaatta gggtttattt atgggagagc aaaagattgg atatttattg attggtctga
     5341 aatggataaa gaaggaacgc ttgctgcaga acaagtctta ttactgaaag cctatcattc
     5401 catgatcact tgcggagata tcttagctga aacatatgga gagaacgaaa attatcatac
     5461 tcatctctat caagaaaaat atcaactatt gaaacaaaac ctatttgact acttttggga
     5521 tgaagaactt ggcgctttta tcgattgcta tgaatctggg agaagaaatg tgacgcgtca
     5581 tgcgaatatt ttcgctgttt tattcgattt tgctaacaaa gaccaacagc aatcgatctt
     5641 aaagaatgtc ttattgaatg acactatcac acagattact actccatatt tcaaattcta
     5701 tgaacaagat gctttatgca aactcggaca gacgaatatc gtctaccaaa cgattcttga
     5761 ttattgggga gggatgttat ctcatgatgc ggtaactttc tgggaagaat acgaccctac
     5821 tcaaacagga aatgaaaagt acagtatgta tggagatcct tatggaaaaa gcttatgcca
     5881 tgcatgggga gcaagcccaa tctatctttt aggacgctat tttatcggtt tgtatccaac
     5941 aaagccaggt tatgaagaat ttatgataca acctaatcta gattcatttg aaacactgaa
     6001 ttgtacgcta cctatcaaag atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt
     6061 gattacttct gatagaacag gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct
     6121 agcaaaacag ccacttgtct taacaagcta ttcaaaaata aatgcataaa acaaaaaact
     6181 gcttgcgaca tatcagaaga ctttaaatct tcttctgata ttgtccaagc aatttttatt
     6241 ttgaagtttg cttaattctg ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga
     6301 aagctagata atacgacaag taaaaagaaa gcgatgattc caactgtcaa ggctatttga
     6361 ccgtattctc ctagtttccc aagtacaagt cctggtacta gataatcagt atcggcaaat
     6421 gtagaagaag cgattttaag atctcctaat actggaagga taaataatgg cagccaacta
     6481 atcaataagc tattgaaagc agaacctaat accgcccctc ttctaccgcc agtcgagttg
     6541 ccgaagacac cagaagcagc tccgcagaag aaatgtccta ctaccccagg aatgataact
     6601 gtagtttgca agcctatcat agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa
     6661 aagccaatta atacagcatt aggtgcatat ggaaaaacga taggaacatc taatgctggt
     6721 ttagaatcgg gtacgagttt tttagcaatc ccttggaatg ctggaacgat ttctgccaac
     6781 accattcgaa ccccttgaag gacgataaca aacccagctg tgaatgttcc agcttggatc
     6841 agtgcaaaga ttacagcgtt gtctccattg cttagttctc tttcaacaaa tccaggacca
     6901 gctaacaaag caagtgcgac atatacgatc gacatcaaga gcatgatcga tacagtagaa
     6961 tctcgcaaaa agctgaaact tttaggaatc tttatgtcct ctgtagaacg gtctttgttt
     7021 ccaaaaaact ttccgatcca tccactagaa gcataaccga tatttccggt atgccctaac
     7081 gcaacttgat tgtttccagt gatgttcttc ataaacggtt gacaaagagc aggcgtaata
     7141 gttaatagtg ttccttcgaa gaccccgcct aataagatca tccacaaatt tgcagtaaac
     7201 cccgcgctta ctaaaatgac tgctgttaga caagaaacat aaagcattgc ttgacctgta
     7261 agaaaaatga atttatatct tgtgaatcta gcaagtaaaa tattcacgat catccctact
     7321 agcatgatca aagcagtagg tgtaccgtat tgttgcaaag caatcgccac tactgcttca
     7381 ttgctaggaa ctactccttg agtatgcaga gctgtttgga acatcgaagc aaaaggggtt
     7441 agtgaattac tgattaaacc agcaccgccg atcagtacta ggaatccgac aaatgttttg
     7501 atagttcctt ggatcacttc tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca
     7561 atcaaagaaa cgaggatgga gggctgactg acgattgaca caaacaaatt caacaattta
     7621 tccattgata gttcctccct tgaatcattt ttattctttt gacaagatct tcgttacttt
     7681 ttcttttact tcttcgctgt caatgatcga ttgcaaaggt acgagctttt catcaggtat
     7741 agaagttagc gtatctttca aagaatgctc aatgaagaaa taatcagcca tatcgctaga
     7801 tacacttcca acatccgtat gacttacttc aaactcgtcc acatctgctc caagatctgc
     7861 aagtgcttgt ttgatgttca tctctaccat aaaacttgtt cctaatccac tttgacatac
     7921 tgcaaaaatt ttcatatcat tcgtctcctt ttcttatgat cgctaatatt tcttcttttg
     7981 ttgatgcgtg taataaatct tgaagatttt gtttattaga aagtagcttt gttaaatttg
     8041 ccaaagcccg aagatgtgcc tcattatcga tagcagctaa acagatgaac aaactaatgg
     8101 gatgggctgg attatcaagc agatccacag gttgttgaag tttcagcaaa gacatcccta
     8161 cttcgtttac gccatcttct ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga
     8221 taaacgcacc atactgctcc acgcgctcga tcatagcttt gatataactt tcttctattt
     8281 tattttctga taatagcggc tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt
     8341 gatccgataa ttggatcatt tcttctgtta ataattcaga tagcatagga cgttgatcct
     8401 ccttcttttc taaaagtttg ttcattttac gatttaatac ttgatagagc ttttcttcgt
     8461 ctacgccttc ttttaattcg atataaggca gtagcgccga caacaattct tgggcagaag
     8521 gcattgaaaa tcctggtatc agccaatctc tttgtacttg attgatcagc tgattttttt
     8581 ccaagtcagt catgattgga tgcagaacat agagagactt tttcttatca gtaagtgtga
     8641 gaggtacagt agaaaaaata atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag
     8701 ctgaattagt ctctgtaaaa aagatcatcg ggaataattg ctgaagttca ctttttaaga
     8761 tcaatgaaga gctaatgcca cttggacaga cgatcacggc tcttattttt ctatttcttt
     8821 cttctgcgtc cgcttttctg atctctccgc caaatagtat agtgaaaaaa ccaatctctt
     8881 cttcagggat gctcttccct attcgtcttt ctaacggaag taatgctttt cttgtcattt
     8941 cgtagatgga agcatattga tgcttaattt gatcgatcat cacattaggt aaataaaaag
     9001 aataggaaat acgaaaatag gcagggacca agtgataaaa gacattcatc aataattcac
     9061 gaaacgaagc aaattctacg gccatcagga attccatgcg atggatgact tctgaagaac
     9121 attccagcaa aaaatcaagt gcaggctcct ggacatctcc ttcaacgatc gatgctaagc
     9181 aaagagagaa aaatataatc tccgaatttt gatgttcgga atagccaagg ccagtcaata
     9241 cttgtcgact aatgtcagtg actgcaagag aagccaacag ttcagcatct tttaccagtt
     9301 ccatggatgt ttttccaatt cgaatggttg tcaaactcaa ataataagct aattcatcca
     9361 ttcgatttgg tgcgaatcga agatctgata gtgaaatcgt tttatctagc aaatctctaa
     9421 ctttggcgta ataatcaatg gaaagggttg cggttaaata cgtgagccca aatcttccag
     9481 caagtgactg catcaaacga gcagtgcgat tttttgcttc tcttcttaat attgcttctg
     9541 accctagcag cctaaaacca gttttcctag tatacgacag ctgacaatct tccacttcta
     9601 attcatttct tatctttttg atatctgaca ggactgtccc tttagaaacc tgaagaaaat
     9661 cttgaaaatg aaaaacagaa acagacgttg cagtcgaata aaccatcaaa taaagaagat
     9721 ctcttcgctc ttgttcggta tacacagctt caaatcgagt aatatccaaa aatttttctg
     9781 cccagtcgat tgctcggaat ttctttatac ggatacgggt atcatccaca tattcggcaa
     9841 aaggagttaa ttgtttgatc gcctcgcttg tatttacttc gatgatctct gctgcttttt
     9901 ctagactgat ttctcttggt gttttcagga gataactaat tttgtagttc acagcgctgt
     9961 tcacctccta cttatattct agtatggtag cgcttttaaa tcgaggggtg tttggtacaa
    10021 cttacagttg gatttttttt acgactgtac cgaacgattt ctatcgaatg gatacagaaa
    10081 aaaagcaggt gaccaaatgt ctcctgcttt tatgaattga tttcgtccga tcaatgatga
    10141 tcatgtttat tttttgaggt cagttcttca aatttttctt tgacatcttc aaccgctttt
    10201 tcgatatctt tttcaaagtg attgggctca ttaggctttg tttcctcttc tacagaattt
    10261 gaaccagata aagctgtttc tttattttct gtttcaccag agtttgtgcc agctaatgat
    10321 tcttctttgt cttctgtttc accagaatta gttcctgcta aagccacttc ttcttctgtt
    10381 ttaggggcca ttgaacctct taatcgtttg tgtttttcac cgtcataatt tgtcatgttg
    10441 gttcctccta gtagttctat actttttcat cgtacaataa gacagtttat gcgtcaaaaa
    10501 acaaacccga ttcttacaaa tatgtcatat ttttccttct gattcttgaa tgcttttgat
    10561 tccttcttcc aaaattttca tttgtaagat tgtttcttca tctttgatga ctttttctgc
    10621 gccattaact aatgtctcgt aaatcccttc gtagacacgg ctgtaatcac caacctcgga
    10681 aattactttt tcttcatgat aactcccttg ttcatctata taggtcaata caccataatg
    10741 ttcgggcaaa tcgataccaa agtcttcatg atcaggcatg taaaacattt tcagatgttc
    10801 ttcttgtcga tcttttgttt gtttcacgaa catccctttt tttccgtaaa cgacgaaact
    10861 tggacgttct ttgatgcgga aataactgga ttttatcgaa attttcatat ttccatagaa
    10921 aaaatccaga tcgaagtaat cattcattcg tcctttccct aaaagttgac gtacatcata
    10981 gctgatatca tctggttcgc cgaaataaga aagaacttga tctactgtat gacaagcatg
    11041 tccatataaa tagctgttcg cagtagaaaa ttcatgcgta ttttctggta cttccggtct
    11101 gaaataatca taatgcattt ctacttctaa aatatcccct aattttccgc tttcgattac
    11161 tttttggact gtcaagaagt cagaatcgaa ccgtctgttt tggtaacatt ggataaatag
    11221 acctttttct tttgctaatg caaataactc ttctgcttcc tttgacgttt ctgcaaaagg
    11281 tttctctact agtacattct taccatgttc gagtgccatt tttccatatt cataatgaaa
    11341 ttgactaggt gtagtgatga caactaattg gatctcagga tcatcccaaa gatcagacag
    11401 ctgatcagta tattcgatgt cttcgaatgt tttccacact ggttcagatg gggaaaaaat
    11461 ttttttgata ttgattttgt tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt
    11521 actctttcca ttgccaagat aagcgattgt aagcatgagt ggtgcctcct tttattgatg
    11581 ttttcagtat atcctagaaa agcgcagaaa gtatttataa tagctattct ggtaaaaaag
    11641 ttcagaaaaa cacttgttat cattcggttt ggtgaaatta atgttcaaag taaggataaa
    11701 aatcaaaaag atagtagcgg aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga
    11761 tcatgcttat tgaagaaaaa ctaacaaaac aagagttgtt taccacgaca gagaaacgga
    11821 ttgtcgatta catccgtagg aatatcgaag ctgcagtcta catgacgatt gaagaattag
    11881 caaaagctac gtatacttcc cattcagcta tcattcgttt atgcaaaaaa atgggattca
    11941 gcggatttaa agaattccgt ttcgagctgg ggcgggaagt tcatcaattg attgctcagt
    12001 tcaatcaaac gattgatcct aattttcctt tttcaagtga agatagcact cagcaaattg
    12061 caaagaaaat ggcggattta tcgatccagt caataaaaaa agcacaattg cagattgaac
    12121 atgaagattt agatcaaatc ggtaaagtat tgaacaaggc acagcgaatc tttctttttg
    12181 caaaaggtga ttctcaaatc acggcaagga aattccagaa taaaatggtc aagttgaata
    12241 agtttttgat catggctgaa gaatacagtg attcttcttg gaatgcagcc aatttgacta
    12301 gcgaggattg cgccattttt attagctaca gcggtcgcat ccatcattat gaacgaataa
    12361 tgacctattt gaagcatgta gaggccccta ccttgctgat taccggaaat cagcattcag
    12421 aaatggccaa acaggctgat atgtgtcttg ttatatcgca ggaggaatat gattttgcta
    12481 aagtagcgac tttctcctct caaattgcct tcgattacgt attaaatacg ttattctcag
    12541 tgatatattc tcaaaatttt gaagagaata tccgcaactt gaaaaacaaa caagaattga
    12601 tgcaaaaagg ccttcttaaa gaatatcgga aaacttaaga aaagatcctc atttagtaat
    12661 aagaaagata gtgtttgata ggctctcttt gatatcatca taaaaacagg ctgaagtttt
    12721 ctttttcaaa cctcagcctg tttgtttacg gaacgattcg aatagatata ttatagaaat
    12781 gaggaatagc taacttctgt ttcttatttt gaacgattga cgaaaaagat atataagaag
    12841 gaggaaaatc agtatacatg cagctaacaa tccatacagg aaaaatcgtt tgttctcgga
    12901 tactttctgt acttcttttt gaacttcttt tgcatgataa ggaacccggt gccctctgac
    12961 aagtaagcga tgactgttga ccatataagg tgtacaagtt aggagtgtca cgaaatcctg
    13021 ccctttttcg attctcagat cttttgtatc agtcggttct accgtcttga tctgatcgac
    13081 ttgataagcg agtgtccgtc cattgatttc gatatagaaa gaatcaccta aagtcagttt
    13141 cggtaagtct gtgaacaact ttgcttgagg cagtcctcga tggctggaaa taacggcatg
    13201 tgtattttct ccccccgtag gataagacgt tccttccaat aatgaggcac ctttttctag
    13261 cagcacagta ttcgtttcat caaaaattgg caaatggaca ttgatctcag ggatcgtcaa
    13321 aataccgatc gtatgtttct caaaataggt tctatccttt ttcgcaggtt cttttttctt
    13381 agtaaaggga tctgcacctg gatttccacc ttcttttgct aattgctggt ttttctttgt
    13441 catgttttct tggattttag ccatgacttc ttcattttct ttgaccgctt gttgctgata
    13501 atgagagatg atctgctgat ccagataatt attcagcgcg tcgctaacaa atggataagc
    13561 tagtatccct attcctgaga gaagcaagaa aagcatcagc agatctagaa gtttcctttt
    13621 ctttggtttc atttgtgttc tccttgtgat gactgtttga taataaaaga aaaaggagca
    13681 tttgcgtaat ccgctgctcc tttttccaat tcttgcaaaa agactaaatc tgactgtgct
    13741 tgcgtctagc aaagtacagt ccagcaatca gtagaaggac tactcctgca ccgatataca
    13801 catagattcc cttaccgcct gttgaaggaa gtgtaccttt gtgtttattt ggtatttttt
    13861 caggagaaat caactgtcct gctgttacat aagattgttc atcgatagta aatgctacac
    13921 ggtttgttaa tggaacatat ttttctggcg ctttcgtttc ttccagatag tacgtgccat
    13981 attttagacc tgtcacatcg attaaaccgt tacttgtggt tgtaaaaacc gttgctgatt
    14041 ctttcgccga tacccagctg acggcttttg tggatgggtc gatcgataaa tatttcgctg
    14101 tgtcactatc ttgatcacga acgacgaatt ctgctccagc aagtgtttgg tctgatgtaa
    14161 cgtcaccatc tactttaaca aatcgtttgc ccccagtaac gacatcgact gacggtggtg
    14221 tttgatcatt tgtatgcccg ttatcgacat ttgcttggtt agaaaatcct ttggttggat
    14281 ctgctttttc gttcaaatgc atatagtaaa cgaatttcaa tgtaccgcca ggagttaatg
    14341 aaggaatata attcggatca actgaaaccg tgaatccgtc tgtttgctca gtgacagaat
    14401 aatttactgg gtcgatttct ttatttccat catataaggc ataagcatac gttcctgaag
    14461 aatcattatc aaatgttaaa gcagcgtcat gagtatcgat aagtttgaat gttgtgtact
    14521 tgttttgcgt tccttcttta tcagcgatac ctaatgggat attgacagaa atctcatatt
    14581 gaattttttc accgattgcg acatcttttc cattcaattg aggtgttgtt ttatcaactt
    14641 tagacgtatc atttttgatg gtcttttcta ctggtgtttg gctatttgcc aggatctcaa
    14701 aaggcgtgat tgtttgcttt tcaatcattt ccgcattatc aggagctttt acttcttcta
    14761 aattatattt tccaacttct aaatgattaa cgatcaactg gcctttttca atagatgcct
    14821 cggcaaagtc attgttgccg atgtcataag aatgaccagt aatgaaatgt ttggctttgg
    14881 tttgatcagt tgtccaagtg tacaatccat ctgtgacact ttggatgtat tttttgacgc
    14941 ttggtgttcc ttcttcttta gaaataataa attctgctcc atttagtgct tcgttttcgg
    15001 cagtaccgat ttttgtaact ttcaacgttc catcattacc gactgtattt ttagggtaga
    15061 gatggatagt atctagttct tctgtcccgt atttataaga gccatctgct tgtttgatca
    15121 tctcatatac agggaaagct aaaaccatgt ttgcggcagc tgacactccg tcttttggtt
    15181 cttctttgat cgtgtagact gcatctttcc cattttgttt tttaggtaaa gataaagtga
    15241 catttccatc agcatctgtc gttcctgaag caactggtgt accaggagtc aaagactgga
    15301 ctgcttgttt tgctgcatcc acggacgctc ctttatctcg ttgcgcataa aattcttgag
    15361 tgacgttata aactgaaaat gaaatatcgg ctaatccttg gtattgatcg aattcgctca
    15421 tttctttccc gctgttttgg attaaaggat cgggtaaatc agtcattttc tttttatgaa
    15481 ggatgacttg agctgctgtc tcctctgcaa acactttttt tgcgccgaag ctgtttgtga
    15541 gtaatggtaa aataaggaaa aggactccta acataacgtt tattttttta tgatttttca
    15601 ttttttcact cctgttcttt tttttgataa acaaccagat tgcaaagctt gcgaatacca
    15661 taccaacaac gcctaaaagt gtatttttcg tttcgtttgt ttttggaaga aatggacggc
    15721 tattttcttt tttattgact actttcaata tactgtccgc ctctttatcc tcagaaagaa
    15781 aagttgaaat ggtaaacgga atttctgttt cacttaaaat ataaccagaa ggtgcttcga
    15841 tctcttttaa tcgataactt cctgttttca acccggaaat tgaaaaagcg ccatttttat
    15901 cggaaatcag gtgaagcgca tctgattcgt tctttgtcca cttgtatccg ttggctgttt
    15961 gctggaggta atttcctgct ttgtttttaa taaggaatac tgcgccagct aatcgtttat
    16021 ctggtttatt tgtctcgatt ttgacaaatt gcttactccc tgtatataca gagactttct
    16081 ttgtactgac accaaaacca aaatccagtg ttccgttgtt gttgatttcc ttgttcgcct
    16141 gtgctgtact gcttagctgc atctgataag aaatggtcag tttttttcca gcatattttt
    16201 ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg tttcttgtgt atgacaaagc
    16261 cttctgtcag cttttctcca ttcgatgaaa tcgttaaact attaggtaaa aacgttaatg
    16321 cttcatccgc actatctgac aattcgaact tttgatagtc aaggatattt acaggaattg
    16381 tcgtatgcaa ggaataagtg attttttcac caatcgtaaa atcatttcta ggctcttcta
    16441 ataccttctc aaaaggtgga tcagggtagt cgttttcttc atttttagga tacaaatgga
    16501 tagttgaaag tttttgattg ttttgtccat gtacaggaag aacaactacc atattctctg
    16561 ccttttcttt gacgacttct ggtactttgg attcaatgaa aagatacgct ttatctcttt
    16621 tttcttgatc tttagcggcc agagaaaaag aagcgatacc atcttcgttg ttaagagttg
    16681 tagttgttgc ttctgcggta aacataccgg aagacgcacc gttttttgcg atctctgctt
    16741 gtgcttcttc taccgttttg cccttttccc gtagatggta aaaagaatct gtgacatcat
    16801 aaacttggaa tgtgacacca tttaatcctc gatacgtttg taataaagct ttttcttgtc
    16861 cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa aagcaatttg tgtaaagtaa
    16921 tctgaaccat ttctgtttct gtttctgttg ctacctgagt aaaagctggt ttaaatagta
    16981 acaagaagag acacatacta agccaaccaa gttttttcat atttagctgc cccctctttc
    17041 ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca atcatcccta ctagatagat
    17101 tcctaaacgg ccaattcctc ccgtttcagg taatggtact tttgcctgat tcgtgatgtc
    17161 taaagaaatc tggttgtttt tggctcctgg tgacagaaca gattcatgat cttgtccatc
    17221 cacttgaatt gacccatctt cgtgtataac tatagtaact ggctctttta gaccttggta
    17281 tccttctggt gtaaaagttt cggtcagcgt ataagttcca ggttttaaat tttcgaatag
    17341 aaaggtatct gtttcttgtc catcttttgg cgattctaag tccatttctg gtccctgcag
    17401 tctgaatttt gctcctttta atgtctgtcc gttgtcttct tttttgtgta ccgttagatc
    17461 aaacgctttt agtcgatttt gatgagtgag cgtccataca ttatttgtca tctcggtatt
    17521 ttttcccgtg taaatgattt ttccatattt atcgatttgg aattcataat ttcccggcga
    17581 agtatcatat ccggcaggac cagctgtttc aacgactcga tatttaccag gtgtgagtga
    17641 atcaaaactg gcaagacctt tttcattcgt tgtttggctg tcaattggct gataagtacc
    17701 atttgcttct tttttctgta gggaaaatgc tgctccttta agatttatct ctttgtccgt
    17761 cccttgcatc gcatacttac gtactgctac aggaacttca acaaaaggat tttcaatagt
    17821 caactggatc gtatcatcgg aagtagtgac tgtttttcca tcaatggtta ccgtaccatc
    17881 agaagcgatc ttgatttccc aagtagtctt tttgcttaat ccatgccctt ctggagcttt
    17941 tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg acattttctg gaagagaata
    18001 ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa tctcctgtta atttgaaaac
    18061 agcgccaata agatcctttt cacctgtaga ggaatttttc gttattttca agtttaacgg
    18121 tgtgaattgt ttagtatttt tccatgtcat tgcatctatt tgttgagaat cgtatcctgg
    18181 tacaggtaat tctttgattg tttggtaact gaatgcttga ccttgattat tgtattgagg
    18241 tagtgataat atctcttgat aactttctcc gctatttgca gataatttgt caatgtctgc
    18301 acgttcccac gtatttgttg tatctttagc tggtttaatg attcgaatat atccgttttt
    18361 ccaagctgca gcatttgtcg tatgttcccg ttgaatctca aaagtaactt gatctggacg
    18421 atcagctaga ttgttgtcaa attcttccca taactttttg atgtgaagac tgactccggg
    18481 agctttagca gatggtatac cgaattcagc taattcattg gtatcaatgt ttggctggaa
    18541 agttgtcctg ccgttcattt gataccagaa atttggatgg aagtcctcat tttctgtttg
    18601 gattctcact tggtaatgga tttggatttc ttggtctttt cctaaataga tctgattgct
    18661 cttgatggta tttccttcta tggaaataga tggagtgacc gttgtaggac ttgtccccac
    18721 actcttgact gaaagtgtac caggctgata aatgaatggt tctgcgattg gatcatttat
    18781 ttgtccattg cttacagttg ctgagatctg tacagctttt ttggctaggt attcagagat
    18841 atctgttgca tgatcagctg attcatagta aagatcgcct ttttcatctg atgaaaccat
    18901 ttgtcgcata cgagactcta cttctgcttt tgagagacca gctgccggat cgctttgaag
    18961 ttggatgcca agaccatgta tttcgattcc tcgttctttg agtgccatcg cttctccgat
    19021 ggttgcgata aacgtactgt cgattcttct ggatagattg ttttggtcag gtgcatcata
    19081 gattcttgag attagagaag tatttcccgg ccgatcttgc gtattagaaa actgagttcc
    19141 gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact ttataggaaa atgttggtac
    19201 accatccgtc agcaaaacga tcactttttt atgtccattt ggaacggata gcatgcttcc
    19261 tgcatctctt agtgcttttt gtgtaaaagt accaccattt gtccgtgaag gtgtaatgga
    19321 ttttacttga tttttcactg aatcaaatga acccatctgt actgcaccgt tactgtagct
    19381 atatccttcg cttgagtagc cgacatatcc catattgatt ttgtctgtga taccgctatc
    19441 tgctaaagta tcgacaaaac gatcgacacc aatctttact tcaccgatcc gattattgtc
    19501 gttcatactt cctgaccagt ctacgaccaa tacgagatca agaggcgtga tatctttttg
    19561 tacatttcca cgagcattca aataaacatc aaacaatccg ggtgtacttg tttcctttgc
    19621 atactttcgt atcgcatagt ctgcttgatt accggttcct ccatattcga tatacgaatg
    19681 ggtccgatca ttaggatctc cattccaaga atttatacca tcccattgat ttgttccgtt
    19741 tttattgcct tgatgatcaa gaacatttgt atttcctgta ggttgccaac tggctttagg
    19801 ataagtgccc tgatcatctg ttgtatattc tgtaggaatc gtttgattag atggcacact
    19861 cacagctggc aaggataatg taggcaatgt aaatgggctg acagaatttt cgttttcttt
    19921 tgatgattct tcttctattt cgtctggctt tggtactgac acatctgtat ctttcttatt
    19981 ttctgtctca tcaataatgt catattcttt attatcgacg atcagcttgt ttgcctggct
    20041 gtggtaaagt gctggatcga ttggaaaact cagatcgata gtttgtttag tgtttgatga
    20101 gatttccaac gtaattttcc cgctatttac ctgataagca tagccatctc cttcttttct
    20161 ttccacatca cttaagtaat tttcgactag accaatttct ttttctatct tttcagcact
    20221 ggtgttattc gcatttagct tgatgttcag tgcttttttt ccagactgat ccgaaatgac
    20281 ttcagctgaa attgtttcta aaactgttgg atcatctgta gtgtctgcta ttgcagataa
    20341 tggcacaaag tttgataaac tcaatattat cagcatgaaa ataactttca gtttcttccc
    20401 tgtggtcgtc atttttcccc tcctttttct ttttttctgt tttgacgtga atagtgaaaa
    20461 ttattaaaat aaacaggcgt tacataattt tatttatttt aatatgataa ttattatacg
    20521 aagggcgcta aagggaatca caccaatttt cataccaatt ggtttcattt atgaagttga
    20581 ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt gagggagaaa aacatatgat
    20641 caggttgttg gaattaaact atagaaagat ggaaaaaatc attcgaattc tgtccatttc
    20701 tgacaagtgg atgactaaaa aagaacttgc caaacgaatt ggtagttcag aatctacatt
    20761 tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg gatgtaacga ttcaatcttc
    20821 acaaaaattg gggtatcgtc ttgaacattt gaatgtacca gcttatttaa atgttctgat
    20881 ggaaatgtcg caaagttcta caaacagtcg tttattaaat gagattattg tcaatccggg
    20941 taaaacgatt gagtattatt gtgaaaaaat ttccatcagc cggtcttctt ttacacgtaa
    21001 gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta aaagtgatcg ttgaccaagg
    21061 ttttcagtta actagcctta aaagtgagct ccagttgcgt atctttgtta cctttttctt
    21121 tttaagttac tacggaagac gcacgttgcc atttccaatc gaaaaaaaga agatcaaaga
    21181 aattatggtc aagaataatt gtcagatgaa tttgtttcag gaagaaagcc catatgagca
    21241 atctttcttt attatgtatt ttgttgtgca tctcatgaga gaatcccaag gaactccatt
    21301 tgaatggaaa gctggagact cttatgtaca gttgataaat atcgataaaa aagacttctt
    21361 aatcttgaaa aatctcttag gaaaattgaa gtacagcagc tatataaaag tagtgaacta
    21421 ttttgtcaat acgatttttt cagaattttc ggagaaagaa aaaaatgaaa tcagtgagca
    21481 gatacgaaaa aatatgtcct actgttcgtt ttcatccgct gattgcaaag aaaaaaacga
    21541 agaatatgtt gttcaattat taacgcaaat ttattttttc tcccgtattt tcccatacga
    21601 tacgactcat ttgacgatcc gtgcaactta ctttgcacag cagatgcaac accgccagat
    21661 acgtctatat gctggatttc gtgcagaact tcaattttat tccactctac tgaatcagaa
    21721 tctgatttat tatttatcta gctatatctt ttggatgctt atcggtaatc ctgaaattca
    21781 ccattatact tctgataaaa aaatactgat tatcagcgac ttaagtcttc gacatagtca
    21841 atatattgaa gaatatatca gtgatattct tgctgtgcat aaaattcact ctgagacaac
    21901 tgctattact gaagatcagc tgtcgaaata caatcttctc gagtatgatc tgatcgttac
    21961 gaatcaaccc attttgaatg cccatgttcc tcatatattg attgatgata gtgtgtcatt
    22021 cgcaaatgaa gaagaactca tccgcttatt cgagttatga ataatcaaaa aacagctatg
    22081 tataaaccac cctgataaaa atttcaggac aaatacacat agctgtttct ttttattgta
    22141 ttccttcagc aatccaacga gatttatagc gaaagtcttt taacaactct tcttttgtcc
    22201 aattatttgc aatgagatcc gtcaaagtgt tcattacgag ggtcatggaa aagtatagga
    22261 aactaactgc ttcttccgac gattgaatgg agaaatgatt gattcttaat aattccagcc
    22321 atttttcgtt cattggagaa tcagccgata gttctctacg tttggttgtc tgctgatcat
    22381 ttcgctgggt taacaagtat aacctttgcc aatagatact ttcgtgatct aagtcgcagc
    22441 accactctaa atatttttcg atccccaaaa agaaatgttg tttgttttcg ctaatgaatt
    22501 ttaagatatc ttgatggact atgtgagaac tgcttttgat cgtatacaaa taagcatctt
    22561 ccaaatcttg aaaatattta taaaaagcac cgcgcgacat ccccatcttg tcaacgattt
    22621 ctgagacttt gacttggctg atgtgctgtg agcaaaatat ctccagtaat aggtcatcga
    22681 ttctctgttt ctttgcgttc gataaattga aaaaagtttc ttttggcatc cacatctctc
    22741 ctttatctct gattatacgt ggatagccct tcattgtcat gaaggattca ttccttcttt
    22801 ttctcttctt ctgtcaagta cccatcttcc attcgataaa cgcgatcact ccattgaatc
    22861 atacggacat catgagtaac cataatagtc gctttttgtt tctcatgagc ttctttaact
    22921 agtaatttga cgacttcata tgcatgatct gtatccaaac tagctgttgg ttcgtctgct
    22981 aagatcaagc ttggctcatt atataaagcg cgagcaattg ccactctctg tctttctcca
    23041 ccagaaaggt ctttaggata actattttta agtgactgga tatctaaaga ttgcagcaag
    23101 gaatcgattt ttgttgaatc ttcttttttt gaagtaactt ggtcaactag ttcaaattgt
    23161 tcctttaccg ttaaaaaagg aatcaaatta gagctttgga gaataaatcc tatttcttta
    23221 aagcgtaaat ctgctcgttt tttttctgga agctcagtta gtgagtgacc gttgatcact
    23281 atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg taaggaaagt acttttacct
    23341 gaaccagaag gaccaatgat gctgacgaat tctccttcct ttacataaaa atcaattgct
    23401 ttcaaagctg agacctttgt atgaccagaa ccaaatgttt tggtaacttt tttcatcgtt
    23461 aatatttttt tcatttttat taacctccta ttgctgtaat tgggtctact ttggttatcg
    23521 tacgtatcga aaacaatccg cctagcattg cgacaatgat caaaatccca ctatatagca
    23581 accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg taaaatcaga ctagtcaaat
    23641 aagcaaacaa aagagcaagc agaacgccta ccaccccaac aataaaggac tgtgctacta
    23701 aagatttagc gatgaagaaa tttttgatcc cttgtgcttt cattactcca aaaatagcag
    23761 tcttctgaag tgtcatcacg tacatgaaaa taccgaccac ggcagcaacc actaagaaca
    23821 aaaagtatat catcgcattt aaggtcatat tttgggcaga atagccagga atactttcaa
    23881 tgaatgtccc aatagttaat aactgtagac ccttcgatgc tttttctttc gaaattttag
    23941 ctgaatcctt tattacgatc ccattgatcg gctgttcttc ttcagaagca aacggctgtt
    24001 gtccatactt caaagcagtc cacgtatcta gggacgcata gatgacggga gaaactgtgt
    24061 aataagtttc aggaaagata ccgacaatcg tcaaaggatc gtcgtaactg ccaattttga
    24121 tcgtatctcc aattttatag cctgaatcag ccagattttg agagatgatg atttcattct
    24181 ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa aaatgcttct ttcgttgtgc
    24241 cgaaaactgt aacattttct ttgttttttc ctttgattgc tccgctgtac aatccgattg
    24301 gtgctttctc tcctccttca atgtatttca gatctcctct tgttaattgt gaagctgcaa
    24361 atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc gtcccaatca tcgattgctt
    24421 ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa catgaatacg acatacgcaa
    24481 tcaagaacat aataccgacg atcaagccat atcgtaattt agaatagcga atttctttga
    24541 tagctagaaa catttgtttt tcctccctat gatagattga tagtgacaaa atgtcacttg
    24601 ttgaatagtg taactctaaa gtgacaattt gtcacttgtg ttgttttttt cttatcaata
    24661 aattgtctaa gacatctttc gataaaacct taacatgagt cataaaaaat ttatttaagc
    24721 cttatttatg ctttccatga taaactaaag tgaccaaatc aatgaggtgg gaaaatgatt
    24781 aaactattag tagtagaaga cgataaagta ttatccaata atataaaaga aatactcgct
    24841 gcaatcggcg agataacaca agtttatgat ggtagtgaag gactgttcga gttggaaagt
    24901 ggagtttatg atcttgcaat cttggatttg atgatgccgg aaatgaatgg atatgaagtg
    24961 ttagcaaaaa tacggaaaga aaaaatacag actccagtat tgattttgac tgcaaaagat
    25021 agtttggaag ataagattga aggatttgaa cgaggtgcag atgattatct aacaaaacct
    25081 ttctatcgag aagaactgat catgcgcgtg aaagcattat tgaaacgctc gctaggtctc
    25141 tttgatgaaa atgttctgaa gtataaggat atcagttgtg atttaaaaag caatgaagtc
    25201 ttgtataaag aacaagtttt gcccatacaa ggaaaagagt ttgaattgct cgtttatttt
    25261 ttacaaaata agggaatcat tttgacaaaa gagcagatat ttgaccggat ctggggattt
    25321 gattctgata cgacgattac agttattgaa gtttatatga gcaacttacg aaaacatctg
    25381 cgaagtactg gattggataa agaaatcaaa accttacgta atgtcggtta tatactgcaa
    25441 agggtgtgat gcaatgaatg gagatattac acgaaagcaa cgtagtcgct tttttgcgac
    25501 aaatgtagca gcatttgctt ttatcttttt tcttttggga ttcattactt tacagatatt
    25561 aaaccaatct gcatacagag aaacagatct gtcattagag aaaataaatt ttgattctcg
    25621 aacagtacaa ttagagatac aaagatatca gcaaggcgat ccctttttaa acccgactca
    25681 aacggaacaa aacagaacaa acatggaaca gccattaatg gaaccatcaa atgaccgttt
    25741 taacactcaa gtgatcttgt ggtctaaaaa tggtgagata ttgaataaag cagctattgg
    25801 tgggcgtttc aaccaaatca aagaattaaa aatggatacg aaaaatctga ataccatcca
    25861 aaatattgaa ttaaatgaag acagtggctc atctgccctc tctttccgtt cgattacgaa
    25921 aaaagcggag acgaatcaag atggcgttgc ctacatccaa attttagaaa atacaaatca
    25981 aatcaagaat tcattagcga cttttcgcac gattatgatt ttgtgtatga ttgttttttg
    26041 gattctctct attggtatca gttattactt gtctagtcta agcatgcgcc ctattattct
    26101 ttcttggaga agacaaaaag aatttgttga gaatgcttct catgaattgc gcacaccgtt
    26161 aacgattatt caaaatagtt tggaacatct ttttaccaag cctaatcata ctattattga
    26221 agaatccgaa agcatcgctc aagcattgtc agagacgagg cgcctcactg gattgacttc
    26281 ggatttgctg acaatcgctc ggaatgattc tgatcagcag ctgctttcaa agcaaatgat
    26341 caatacacaa gaatatatcg aacaacttgt taaaccattt caagagatgg ccatcataga
    26401 tggaaaagag tttattctcc ataacgatgc aacattagaa gtaccaattg atgaaaaaaa
    26461 aatccatcaa gttttggtta ttttgctgga taatgcatta aaatatacga atccagaaga
    26521 taagatcatt ttggattcat ctgttaacca taaagaatgg ctgattcacg taaagaatac
    26581 tggcccttct atcagtgaag aagatcaagc aaggattttt gatcgatttt atcgggaaga
    26641 tcaatctcgg gcgaaagaga ctggcggata cgggcttggc ttggcaattg caaaacaggt
    26701 aatcgaacag catcatggac agatttctgt atccaattat cttccacaag gagtcgattt
    26761 taaagtccaa ttgccgttga agcctaaaaa ataatgctta tccctaaaaa agggctgtga
    26821 caaaaatctt gtcacagccc ttttttgttg cgaataaacg gtgttccaga agtagcttct
    26881 tcggaaataa gccgaaattt ctttaagctg accacttctg tcacaacctc tttagcttac
    26941 gcttacgatt ttttattgcg gtcttcaatg tgtacatcac cgcttgtgtc aatatccaat
    27001 tcttctcggc taacatcttc tgtcactttt tcaacatctt catgttcttc tttatgaatc
    27061 ccaacttctt cacgaataac tgtatgtttg cttacatcta cttgctcttc tttgattggg
    27121 atagtaatcg tgtcatcttc caatttttcg tctgttccct gtgaagattg atctgttact
    27181 ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta cttctaccgt ttcagtatcg
    27241 ttgaccacat gtttgtggat gtcgacttct ccagttgtca catcatgtgt atttacatct
    27301 aatttttctt cttttagttg aattgtatca tcatctagat ttggtttgcg atcgatgtct
    27361 tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta cgtccatcgg atcagttcct
    27421 ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg tcgtgccacc tacgtccatt
    27481 ggatctgttc cagtgccagt ttctaatcca gtccccattg tgcttgctgg gctaacagta
    27541 tcttgtgtat ccattgtatc ttctgttgtt tctggctgac gatagccttt tactgtgatg
    27601 acaaattttc catcagaaat atctcctcta tattgatata aaggatcatt ttctggtgta
    27661 gccgtctcag aatcataggt atcaaaagaa aaagtatctt ttattttttc ccataaagaa
    27721 cggtcttctt caccatccat ccgattttca tctgtttcaa catctatacc agacaatccg
    27781 tcaaatccta gtctagatgc tgcttcatca gtagtataaa taacgatatc atctcgttca
    27841 tacccttcgt tacgcaaccg ttcaattact gataatgctt cttccctcgt actatagctt
    27901 ccgatgaccg ttaaattttt ctcttccatg taaattcctc ctagcaaata gatttatgat
    27961 tggtatattg ttacttataa atttattcta taggagacga ttgttttcta caaatattta
    28021 gattggaatt tgattaaaac aatttaattg aaatttgaaa acatttatgt taatgaaagt
    28081 catattctaa taaaatgtca tttgtttcta tatgaatttt tatgaaaaat ttcatataat
    28141 ttccactcgt tgtgatggtt taatttatcc aatccatcaa taagcaaaca acttatataa
    28201 aaaaggatta attctcaata taattgctcc taccatagaa taaatcttca tgtaaagtaa
    28261 tgaagtacca actatttcta atacttcttt aagaattatc ccaacaatat cgttagacaa
    28321 accattattg aataaatgga gagccaatcg attctgaaaa aatgttttct aatttttcgg
    28381 aaataaaaga aagagcctcc tgccattaga acgaaccaaa tccatttcat tgctcatgaa
    28441 gtaaccacat agctaaaaag aggataaatc aattaaaaga tcaaaagaat gactgcacta
    28501 ataaaataac taaaaatgta tcttcttact tcatatcttt tagctagtac ttagttaaaa
    28561 atttgcttcc taagatcaag acaaactgtc tatcttcatt tgatgaaatg cggtaaaata
    28621 tagaaaatga acactattgg agggaatttg taatggacag caaaactaga aaacaactgc
    28681 aagaaaagtt aaagcaacgt acacacttgc tcccttccgt ctttcaacag tttttagaag
    28741 aaaatcgttc ttccctttca ttaggcagtc gctatgaata cgcaaaagat tttcatctgt
    28801 ttactgatta tttgagaaaa gtattttctg aagaagtgga tgatcagctg attatccatt
    28861 tagaaaaaca agattatcag aatttcttag ctaaaatcta tcgacatact cgttcgttcc
    28921 aaactacagc gaaccaagaa acggagcaat tgtttgaaaa tagctattac gggatttcaa
    28981 ggaagcaata ttcattaaat cattttttac gatttttgta tcaaaaagga ctgattgaag
    29041 aagagatttc tcttttgggt acaagtttac cggaaacaac caaagcagct ccgcgatata
    29101 tcgatgcaga acttttcaaa gattttgagc atttgtttat cccgattgaa ggctttcaaa
    29161 caagcagaga agctagcttt gaagaaaaga atagaccgag gaaccttgca attactgcca
    29221 ttcttctgta ttgcggatta aagatccatg aagtagtaga acttaaacgt aaagatgtat
    29281 ctttgaccaa gcagattctt aatctgaata gaaaagaaaa cagactgaat aaagtaccaa
    29341 taccaaaaga agcgatgcca tttctggaga actattttaa gttaacagca caaaatacgg
    29401 acgaaaattc ttttgttttt cgttcctcac atgatcagca aatttctcct cggactattc
    29461 gccaaatttt gagcaaaatt acagtatata aaaacgtatc gccagaacta tgcagaaaaa
    29521 catatcgtta ttatgcagaa ctatatttag atgatgctga cgcggtaaat tatttatgtg
    29581 gtaatcgata cctctcatcc cattcttttc aagatatctg ggaaaagatg gttgattttt
    29641 cttatcacga attagcggca ttcgttcggg tttcttccat ataaaacaaa tgtatgatct
    29701 ttttttcaca ataaactatg ttattatata aaggattatg ttacgattat cttgaatttg
    29761 tatattactt aacaaaagat aatggagtgt gaatgatgga gaccataacg tattcccctt
    29821 tgaacttatt tacgaatttt caagaagctg cagctaactt ttccgagacc ccgattatct
    29881 ttgatgagcc actttcagct ttcccagaat taggacttga aacaacttac aaaaaatgct
    29941 gcgaaagtgt gttgaaacgg gcttatcaat tggctcatct tggagtgaaa tccggagaca
    30001 aagttattat ttataagagt tccgcttttg acacatacct tctagctgtt tctgtgtctt
    30061 accttggtgc agtgcctgtc atgacttctt atcatctacc aacaacaaca atggaagtat
    30121 ttatcgatcg tttagaagac cctttcattt tatttgatga tgagacaaaa aagcgagtga
    30181 gagaaatttc aaatggaaca aaatcaaaac agattcctat tctttattta atggaacaac
    30241 ctgctttgtc tgtttctcaa tcatttttag acaaaaatga aatttcttat atgacccata
    30301 cttctggtac aactggtatc cctaaactta tttgtcattc tgctcattct atgggatggc
    30361 ggacgaaatg gcaaaaaaca gttttcacaa aaatttcgga aaagaaatta atcgcttttc
    30421 acatctctcc agtgcattcc agattcaata ttggtatctc ttctttgatg tccatgggat
    30481 ttcctatgat gcctttagca aacgcacaaa gttcaaaagt tgttcatatg ttggaagctc
    30541 accggccaat tgcactagag acccatccga ataactttgt tcagtggcgt tttactgcta
    30601 aggagcatcc agaagctttt tcgggtatcc gatactatca ctccactttt gatgctataa
    30661 acaaccagac aatgaagaca tttttgcgaa cgtcgatggc acaggatgcg gtatttcttc
    30721 aagtatatgg ccaaagtgaa tgcggaccaa tgattcttaa ggctcataca ctagagagtt
    30781 tgaaaacaag tgatgcaaga gatatgggcg ttggactgga agacatgaca agcgctcgga
    30841 ttacagattc agttgggaat gtcctacctg aaaacacaga tggtcatata caattactgt
    30901 ctaaaggacg ggctttgact tattacaagg aagatgcacg gttccaggaa aatgtttatg
    30961 gcgattggtg ggatagcgga gattacggtt ttatggataa gcatggccac ctatttttga
    31021 aagatcggca agtggatttg atcgaaacga tcaatagtac attagctatt gaagactttt
    31081 tattagattc gttagatttt ttggcagaag tagtgattgt aagggataaa aatcattctc
    31141 cacaaccgat tattgcttta gctcctgata aagaaatgga ttggaatcga tggtgggagc
    31201 aagtccatga acttcctcgc ttgaacgtcc ctatcatccg tgattttgat gcaatcccgc
    31261 gaacagctac aatgaaagtg caacggcttc aaatcgagaa agaattaaaa tcacaaaagt
    31321 cctaactatt taaggaaaga aagatccatt ttggtctttc ttttcttttt gtttttttaa
    31381 attggatgag ataaaaatat gccaattggc tgtttttctt ttttgaaaat ttcgttatac
    31441 tcttattcat ggaggaaaat gttatgaaaa gaatcttgac aattggcggt tctgatccat
    31501 ttgctggtgg aggaatccaa acagatttaa aaacttttga gaattttggt ctattcggac
    31561 taagcgcctt gacttctata ggggcactag atattaatga gcgatttatg ctggaatcta
    31621 tatctttgga attgcttaaa cgacagttag tttcgattga taaaatgact tcattagatg
    31681 gtatcaaaat tgggttattg aattcaagtg aagcaatcat ggttgttcga gacttcataa
    31741 aaacaaaaca aaatatccca atcattttag accctgtttt ggcattcaaa gaaactgaag
    31801 ctgtgatcaa tcaattgtat atttcgcagc tcatcgaaga actttttcca cttgttacga
    31861 tgattacgcc taatttggca gaagctgctt tactttctgg tcaagctctt ccctcttcgt
    31921 taacagaaat gatccatctc gctaaagaac tcactgcttc aggaacaaaa aacattgtga
    31981 tcaaaggcgg cacaaggatg gctggagatg aagctgtgga tcttctctat tcaattgatc
    32041 attatgaggt attttctcat aaaaaaatcg acactacaac agtaaacggg gccggttgtg
    32101 ccttttcttc tgcaataaca gcgaatcttg tgttaggggc agattttacc aaagcagtag
    32161 cagaaagtaa aaaatttgtt gcccattgta tttctaatgg cgtgctgatg aacgatcata
    32221 ccggaagtgt ctggtctggc ggtattcaaa aaggaggaat gagaaatgaa aactaagcaa
    32281 ttaacgatct atgctatgct gattgcgttg acagttgccc tttcattaac aattttgatc
    32341 cctgtaccgg ctacgaatgg atttgtgaca ttatgcgagg ccgggatcta taccgctgct
    32401 tttctttatg gtccaacggg tggattagtt gttggtgctt ccagtggtct attgatcgat
    32461 ttgatttcag gatatccgca gtgggcagtt ttttcattag tgatccacgg gttacaagga
    32521 tatgttgctg gttctttcag caaatcttct actcgtatgt ggggattagg gcttttatta
    32581 ggaagcttag ttatgatcat cggctatggt atcgccggtt ggattttata tgattggccg
    32641 gctggagtgg cttctatacc aggaaacatc ttccaaaatc tgattggaat gggacttgcc
    32701 tttcctataa ctgcaagtct tcaacgtatt caagcacgga agcaatatta attgaaaaag
    32761 aaggttttag aaaatgaagt tgtcagaaaa agaattaaaa gaccaattga ctgaaatagt
    32821 taatgacata ttggctgagg cacacttgaa aaaaggtgat ctttttgttt taggatgcag
    32881 cacaagcgaa gtcgttggag gacatattgg aaaaaattcc agtgcggaag ttggtcaatg
    32941 gatcatccgt acgttaaaag agttgttgga tcctaaagaa attgcgctag ctgtccaagg
    33001 ttgtgaacat ttgaaccgag cattagtagt agaacgaaca gtggcagaag caaaaaattt
    33061 cgaaattgtc tctgttgttc ctgcactaca tgctggaggt gcttgttctg ttgctgcttt
    33121 tgaccagttc aatgatcctg tcgaagtgga acacgtagtt ggacaagccg gtatcgatat
    33181 cggtgacaca tcaatcggta tgcatatcaa gcatgtccaa gtacctgtca gacctcgttt
    33241 gaaaaccctt ggacaagctc acgtgacagc ccttcgctct cgtcctaaat atatcggtgg
    33301 tcctagagcg aattattgaa atgagtaatt gacagagcaa tctgaaaaac ttcctcgcaa
    33361 agtccaacta atgaacttta acgaggaagt tttacttgtt tgttttatat aaccgagctt
    33421 cataaggtgc tagtatgcct ttagcttcaa attctccgct agaggaattt gacaagagta
    33481 atgtccagga ctgatctgct tcttttggaa gttcatagtc agcaagtgat tctgttaagt
    33541 ttaccataat aagatattgt tcatcttcta aatatctttc atagacataa agttgtggat
    33601 gttcatgaag atattcttta taagaaccgt atatcagacc tttattggtt tgtctaatgc
    33661 ggatcatgtt cttatagaat tgcaggaccg actctggttc atattcttgt tgttctacat
    33721 tgatcgtttt ggtatttgga ttagtgatca gccaaggttc atgtgtagaa aaaccagcat
    33781 attgttccga agtccattgc atgggtgtcc tgctgttgtc tctcgttgtt tcacgaatga
    33841 tatccaatgc ttcttctctt gtcttacctt ccgcaagcag ccttttataa agtcttttcg
    33901 aatctactgc atcaacttga tcgatcgatt caaagggcat attcgtcatt ccgatttcct
    33961 gtccttgata aataaatggc gtaccttgca aaaagaaata catcatagcg attgcttttg
    34021 cagacatagc ccaaaaatcg ggacgagtat ctccaaacac tgaaacggca cgaggaacat
    34081 catggttttc catatataaa gcgttccatc cgataccatc caatgaagtt tgccaagcgc
    34141 ttaatgcatg tttcaattta aggacatcta attgcccttc tttttcttgc tgccagatat
    34201 ggatatgatc aaattcgaat atcatatcga aataaccgtc tttccctacc cattgtggtc
    34261 cttcttctgc agtgacacca cttgcttctc cgacagtcat gatatcgtac tcttcaaaaa
    34321 catgtttcaa atcggtcaaa tgttcttcga ttcctgagac attttgaaag ggtgagaaag
    34381 ggttctctgt ggcttttact gaatattcat ctttttttac atgagaaatc gcatctaaac
    34441 gaaatccatc tatcccttta tccaaccacc atcggatcat attgaatagt tcttctttca
    34501 atttctcgct ttcccaattt aagtctggtt gttcttttgc aaaaacatgc agataatact
    34561 gtccagtttc atgagaatac tcccaagcag aaccgccgaa aatcgatgtc caattattcg
    34621 gttctttgcc atctaccccg tcgacccaaa tgtaataatc gcgatacggg ctttctttag
    34681 attttttcga ttcttcaaac cattgatgct gatccgaact atggttgatg acaagatcca
    34741 taatcacttt gatccctaat tcatgtgctt cttttagcaa taaatcaaaa tcttccatcg
    34801 ttccgaatct ctcttctata tcttgatagt cacttacgtc ataaccatta tcgacaaatg
    34861 gagattgata gatcggattg atccagataa agccgatacc aagatctttc aaataattaa
    34921 gtttttcacg gatcccattg atgtctcctt gaccatcgcc attgctatct ttgaaactag
    34981 caggatagat ttgatagcct actgcttctt tccaccattt ttgttcttgt atcatatcgt
    35041 ttcactcctt taacggtccc tcttgattat aaccgattct attttagaag tatccttgtt
    35101 accggtaact agaaacgaaa agctcaacga atctgctaag tgcgttcgtt gagctttatt
    35161 ttatcataga tagcgggcaa tcagttgaat catttgcttg ggaaattcat tcaagtcagt
    35221 aatatctaca aaacgacttt caccgtagat cgacttgatt tcttctttgt cttggccaat
    35281 agctgcggag acaaatagga ttccttgacg ttcatattct ttcaaaacag actggatatc
    35341 ttcttttgct tttttgcctg tataatccgg caatgctttt ggctgaccgt cactaatatt
    35401 aagcaacagt ttggttgttg cggattgtct gtttaatttt tctgctgcta aacgaagtgc
    35461 agcaccatca cgattgtttt gccttggttt catagtgact aatttttcat ttaaccattg
    35521 aaatccgtct tcaaattctt tataagaaaa caaagaagtc ttttctcttg acgaacggtc
    35581 tgctgtatct ccgtaaatca acaatgggat atcgactttc ttagaaaatt cggcaatggc
    35641 aatcgctgct ttttttgccg cttcgatccg atcatcccgt atcattgacc cagattcatc
    35701 gatccgtaca gctaccgcta aagaaggttg ctcatgtggc gggttctttt tgtcaaaatt
    35761 tctcaaatca ccatatgcta ctttgcttgc attgaaccgc tgaccttcgt attttccttt
    35821 ttggtacgtc tctgtttgtt cattatccag taactctaaa atttgccggc taagtgattc
    35881 tactattggc attacttgca tgtgtagctc acgtgcttcc ggcgtttttc cttggaaagc
    35941 tggtcggtga acgatgagac cttctttttc atgaatcgtt tccttcatga tttgtcgcgc
    36001 ttctttattc agttgcttct tgatttcctt atcatgtgct tccatgtcag aagcagacat
    36061 ttcttgagaa tcatctcctg tttgttgttc tttttgattt tttccttcga tatcgctact
    36121 gcgttctttt tgcatttgag gaggaatctc gtttttttct attgtttctt cttgtttctc
    36181 ttctccctca tttggttctt cagcagacaa cgatttcttt tcatctaagc gcagaatttc
    36241 ttcagcattt tcagtgatgt ttcgttcatt tgcttcttcg gcgctaatgg ttctttcttt
    36301 tgtatgctca gattgaggat gaccccattt ttcttttctc aatatgtcct gaagttccag
    36361 tagcaatcca ctgtcagaca aagcttgttc aagaattgcc aattcatctg aatcagtgga
    36421 taatttatac aaaacttttg gataaatcga ctggagtaaa tcttgtcctt gcttataggt
    36481 atttgcccaa taaaaataag aacgcattcc tgctacacct ttgatggcgt tagcttttgc
    36541 agtttcatcc aacaaacgga taatctctgc catctttaag agaagttctt gtttttggat
    36601 catagttttc gccatcgcac gttctgccat gacttctaag gcaggaagat ccattttttc
    36661 cgcatgttgc atccgatctc ttaaagattc atttaatggg cgatttcctt ggtaatttcg
    36721 atttgtagtg atcacaatga cgcaatctgg atggcgacga atgattcctg tcggtaaatt
    36781 caacatccca tttggttcga gcgctgagtt aagtgccaca agaacagagg catctcggat
    36841 gacagtcggc tcttgaatct ctaataaata gcctttttca atcgctcgaa cgatttcaga
    36901 agggtaatag ctgtaatgga ttgcatctgg ataatcagct tcgatccttt gaactagctc
    36961 tgctaatttt tctttagcag aaaaaggatc aatgccgtaa tggttctgga tcagtgtcag
    37021 tacagcttca agtgtatctg tttggtaaat agcttctagt aattcttggt cttgttcctt
    37081 attagaatct actacaggta ataaggctcc gaaaacatct gatttgtcca tatctgcaaa
    37141 acaggtcact tttgtatagg gaagttgcag atcagcagat agtgctttgg ctaattgtgt
    37201 cttccccgac ccggcatctc cttccaaaag tacgttactg attttcattt caggatcttg
    37261 ccaattccct ttgatttcgg aggcgatccg ccattcttct gcacttgtcc gatggctttt
    37321 cggtttcttc cagatcattt gcttttctag ccgtgaaaag gttcggtttt ctgttagtga
    37381 gaaattctcc atgcttctct tccccttttg atcatctctg tctattaata tcctagctca
    37441 tctaaaagac gttttaaaat acggaatcgt tccccaatca gttctccgtc tttttgtagt
    37501 gtatcgtaat acaaaatgat gtcttcatcg atctttggat ttttggtatc tacttctacc
    37561 gtcattcggt tcccttgtaa tccaatgatg tctacgactg gattttctgg atcataaaat
    37621 ttttcataac gataagcatc ttggaagtat aaacttcctt gcgcagctaa gatcgctaaa
    37681 tctaaaatac gtgtgattgg taattcttcc gactgtcgtg accatttttc tccagtatgt
    37741 cgccatacct tgcctgagat atcgactttt ccacgatcat tccactgagc aaggcccagt
    37801 gaaagccctt ttgcatccat atttaatgca tttcttccat caactttatc atagtcttct
    37861 acaacgatta ctggtttatg ttttaaattt gttggtattt ccattatttt aactcctcat
    37921 ccttttattt ttagtaaata agtaaattag tagtttactc atttactaaa ctatatgcga
    37981 cattatagag gattgaagtg aggattgcaa agaaaaagag cttcattgat agaaataaaa
    38041 acaccatctc gttcttttac acgctcatta gtaacatgta gaaagtccaa gatgatgttg
    38101 gaat
//
LOCUS       DAFCNS010000021        38019 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFCNS010000021 DAFCNS010000000
VERSION     DAFCNS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.021"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(451..1761)
                     /locus_tag="LNE23_001012"
     CDS             complement(451..1761)
                     /locus_tag="LNE23_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK6930883.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="LNE23_001013"
     CDS             complement(1773..3191)
                     /locus_tag="LNE23_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6930884.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="LNE23_001014"
     CDS             complement(3431..4999)
                     /locus_tag="LNE23_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK6930885.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="LNE23_001015"
     CDS             complement(5003..5413)
                     /locus_tag="LNE23_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBK6930886.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="LNE23_001016"
     CDS             complement(5410..6333)
                     /locus_tag="LNE23_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBK6930887.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="LNE23_001017"
     CDS             complement(6330..7208)
                     /locus_tag="LNE23_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBK6930888.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="LNE23_001018"
     CDS             complement(7195..8532)
                     /locus_tag="LNE23_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK6930889.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="LNE23_001019"
     CDS             complement(8535..9638)
                     /locus_tag="LNE23_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK6930890.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="LNE23_001020"
     CDS             complement(9712..11220)
                     /locus_tag="LNE23_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6930891.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="LNE23_001021"
     CDS             complement(11186..11878)
                     /locus_tag="LNE23_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK6930892.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="LNE23_001022"
     CDS             complement(11900..12874)
                     /locus_tag="LNE23_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6930893.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="LNE23_001023"
     CDS             13174..14046
                     /locus_tag="LNE23_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6930894.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="LNE23_001024"
     CDS             complement(14504..16702)
                     /locus_tag="LNE23_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6930895.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="LNE23_001025"
     CDS             16934..17926
                     /locus_tag="LNE23_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6930896.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="LNE23_001026"
     CDS             17986..19407
                     /locus_tag="LNE23_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBK6930897.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="LNE23_001027"
     CDS             19400..20899
                     /locus_tag="LNE23_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBK6930898.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="LNE23_001028"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="LNE23_001028"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBK6930899.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="LNE23_001029"
     CDS             22491..23174
                     /locus_tag="LNE23_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBK6930900.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRPP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="LNE23_001030"
     CDS             23176..24012
                     /locus_tag="LNE23_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK6930901.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="LNE23_001031"
     CDS             24023..25777
                     /locus_tag="LNE23_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6930902.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="LNE23_001032"
     CDS             complement(26053..26394)
                     /locus_tag="LNE23_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK6930903.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="LNE23_001033"
     CDS             complement(26462..28633)
                     /locus_tag="LNE23_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK6930904.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28802..29701
                     /locus_tag="LNE23_001034"
     CDS             28802..29701
                     /locus_tag="LNE23_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK6930905.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="LNE23_001035"
     CDS             complement(29772..30629)
                     /locus_tag="LNE23_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6930906.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="LNE23_001036"
     CDS             complement(30614..33316)
                     /locus_tag="LNE23_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBK6930907.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="LNE23_001037"
     CDS             complement(33329..34618)
                     /locus_tag="LNE23_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBK6930908.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="LNE23_001038"
     CDS             34768..35814
                     /locus_tag="LNE23_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6930909.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     SYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="LNE23_001039"
     CDS             35816..37969
                     /locus_tag="LNE23_001039"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBK6930910.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11221 a         7826 c         6751 g        12221 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtcc gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatagtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaag
//
LOCUS       DAFCNS010000022        36567 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFCNS010000022 DAFCNS010000000
VERSION     DAFCNS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.022"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="LNE23_001040"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="LNE23_001040"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBK6930911.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="LNE23_001041"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="LNE23_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBK6930912.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="LNE23_001042"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="LNE23_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBK6930913.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="LNE23_001043"
     CDS             complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="LNE23_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBK6930914.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="LNE23_001044"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="LNE23_001044"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBK6930915.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="LNE23_001045"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="LNE23_001045"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBK6930916.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="LNE23_001046"
     CDS             complement(8052..8522)
                     /locus_tag="LNE23_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBK6930917.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="LNE23_001047"
     CDS             complement(8568..8993)
                     /locus_tag="LNE23_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBK6930918.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="LNE23_001048"
     CDS             complement(9060..9329)
                     /locus_tag="LNE23_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBK6930919.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="LNE23_001049"
     CDS             complement(9340..10935)
                     /locus_tag="LNE23_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6930920.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="LNE23_001050"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="LNE23_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBK6930921.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="LNE23_001051"
     CDS             complement(14488..15048)
                     /locus_tag="LNE23_001051"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBK6930922.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="LNE23_001052"
     CDS             15401..16375
                     /locus_tag="LNE23_001052"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK6930923.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="LNE23_001053"
     CDS             16545..17945
                     /locus_tag="LNE23_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBK6930924.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="LNE23_001054"
     CDS             complement(18023..18745)
                     /locus_tag="LNE23_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6930925.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="LNE23_001055"
     CDS             complement(18824..19330)
                     /locus_tag="LNE23_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK6930926.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="LNE23_001056"
     CDS             19453..21252
                     /locus_tag="LNE23_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBK6930927.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..22894)
                     /locus_tag="LNE23_001057"
     CDS             complement(21320..22894)
                     /locus_tag="LNE23_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK6930928.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="LNE23_001058"
     CDS             complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="LNE23_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBK6930929.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="LNE23_001059"
     CDS             complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="LNE23_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBK6930930.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24671..25309)
                     /locus_tag="LNE23_001060"
     CDS             complement(24671..25309)
                     /locus_tag="LNE23_001060"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBK6930931.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25310..25960)
                     /locus_tag="LNE23_001061"
     CDS             complement(25310..25960)
                     /locus_tag="LNE23_001061"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBK6930932.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="LNE23_001062"
     CDS             complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="LNE23_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBK6930933.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="LNE23_001063"
     CDS             complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="LNE23_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBK6930934.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29103..29843)
                     /locus_tag="LNE23_001064"
     CDS             complement(29103..29843)
                     /locus_tag="LNE23_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBK6930935.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="LNE23_001065"
     CDS             complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="LNE23_001065"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBK6930936.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31214..32152)
                     /locus_tag="LNE23_001066"
     CDS             complement(31214..32152)
                     /locus_tag="LNE23_001066"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBK6930937.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32145..32636)
                     /gene="def"
                     /locus_tag="LNE23_001067"
     CDS             complement(32145..32636)
                     /gene="def"
                     /locus_tag="LNE23_001067"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK6930938.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32651..35059)
                     /gene="priA"
                     /locus_tag="LNE23_001068"
     CDS             complement(32651..35059)
                     /gene="priA"
                     /locus_tag="LNE23_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBK6930939.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35312..35617)
                     /locus_tag="LNE23_001069"
     CDS             complement(35312..35617)
                     /locus_tag="LNE23_001069"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK6930940.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="LNE23_001070"
     CDS             complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="LNE23_001070"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBK6930941.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9885 a         7988 c         6706 g        11988 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt gattcttctg
    21901 ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc tgaagattca ggcgctgttg
    21961 attcttctgt tgaagattca ggtgttgttg attcttctgt tgctgaagat tgtgtagctg
    22021 atgatgattc ttcagtcatt gaagattgtg tagctgatga ttcttctgtt gctgagcttt
    22081 gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc tgcttgttcg cgtgcttctt
    22141 tttcagcttt ttcttgtgca gcttgttgac gagcttgttc tgctagacgt tgttgttcac
    22201 gaatacgagc ttgctctgct tcagcttctg ctttttgacg gttcaagtca gctttcttgt
    22261 cttcagcagt tgcttgttca gcagctaatg aagttttcag tacgttcaaa tcagcttgtt
    22321 tagataaaag atcgcctttt tgagattcca aagcagcttg gttttcagct aattctttta
    22381 gtttagcatc gttttcagct tttttgtctt ctacagcttt tttatcttgt ttttgttgtt
    22441 ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc ttgaacgcgt ccaatagcat
    22501 ctgctaatga atcagcattc aatacagcat cgatgtagtt tgagcttgtg ttgcttactt
    22561 gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt ttcaatacgt tcttgtaaat
    22621 ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa tgtatcttgt tttgctaata
    22681 ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc taaggcatcg atttgtgatt
    22741 gtgcatctgc ttgttgattt ttcaagtctg caatcttttg atcttgctgt tgtatctgag
    22801 aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac ggcagtcaag gtcattgaac
    22861 ataccattac tgctgatatt aaactctttt tcactcttca ttcctccgac tggcttaatt
    22921 taataaatat ctaaatatat ttaagtcttt cttaacgata taaaaattgt accatagcag
    22981 tgtgtcaacg atataatagt tatgttacaa aaatgtttca aaacaacttt taaaaatgac
    23041 aaaactgttc gtttcagaaa atgatcatga aaaagccgcg ttaagtctag catttcttaa
    23101 gataacaacg attctttttc gctttcgacc taaaaataaa aaaagaaatt attcatcact
    23161 gaataatttc ttaaagggaa aaatgaataa tacaaatata atcatgttca acagcagtgt
    23221 aggtcctaaa aaccttgtaa taaaatacgt gttatttact actgctaatt taaaaatcaa
    23281 ctggaccacc atcgtaaaca attcataacc cgtgacaaag atgatcatac tgaaaaattc
    23341 agtaaagatg ttgacatgga taacgtcttt cattacatac atcaaccata caattaaagg
    23401 gagagctact gcatagatac cgataacgcc aatatagtaa gcgtcataga tagccccgag
    23461 aacaatcgtg gtaatcagta aataacgttt ttcaaacgcc atactgcaac ataataatgc
    23521 caatatcaga aagtgggcat ttgacatata ggtgcctttc gaccactccc ctagcattct
    23581 tgttaaatga ccatctatca acatcaaaaa gaacaaaacg attggcagat agtacttcat
    23641 tgtttctttc ttcaacatgg actaatcctc cgctagccgt tggacaatcg tcacaactga
    23701 cacgtcatac atttctgcat aaggtttcac ataaacttcc cgatccagcc cataactatc
    23761 tggtttcgtt ttaataaccg taccgatcgg caagttagct ggagaattcc ctccaagacc
    23821 ggatgtctgg acaacatccc cttctttgat atccgtatca ccagttaatt gggtcaccac
    23881 taaggcatgg agcttttcat catagttttt aagcaaacca aacgcttcgc cattagccga
    23941 agatacccgt actggaaaat gattggagct ttcattagag gatgacagta attcgatttt
    24001 agacgaagcc gtattgacct cgatcactcg gccaatcaat cctttttgcg acatgactgc
    24061 catatttgct tcgataccgt ctttagaacc cttgtctacg atcagcatgt cctgccaact
    24121 gtcaggcgaa cgagtaatga catttgccgt tactttttcg taactagtca gcgtctgatt
    24181 caactcaagt tcttttttca acgcttcgat ttctctctcg taattcttgt tttgctgtga
    24241 aacgtcatca tagctatcga ttttttcttt tagccgctca ttttcggcat aagttgaaaa
    24301 aagattatgg acagaatcta ctccatttcg tacccaccgt actggggcag agattgtttt
    24361 atcaaccatt gcaacactgt cattaacgat ggattggaag aaattgggct ttccttcatt
    24421 cgctcgctgc gcagcggtca cacttagtat cgtcaccacg atgataacaa taaccaaggt
    24481 aataataata tttttattag gattgaactt cttcacaaag acacctcgaa ttaattagac
    24541 tcttgtgttc aattttatca ctaaatgccg tgaaacaaaa ggtaagttgc tcctcttaag
    24601 ctttttttta gcaaataata gattctgtca tttttagcct gaaagacaaa tttttttatt
    24661 tgtttatgga tcaatctttg ctttgaataa ctgcaatcat accatctgaa aaagaaaatt
    24721 ctgcggtatc tgaaagaaat tcgttacttg cataagatgt cggatattct acttctttat
    24781 tttccaatac atacttgctg ccttttaatg taagattcga gacgggagtc aagcagcaat
    24841 aagctaaata cttcatatct ggtatctgtc ttactatatg ctctccaggc aaataataag
    24901 acaaataatt ttgacaatct ctgataataa gctgatgggc aaagggtttg aagcgaggtt
    24961 ccatacctaa ccagagattg gctaaaagat gatccaatct tccgcccgta gctccaatca
    25021 aagtgacttc cgcttgcggg taacgttcaa atgtttttaa gatacctaac tgtgtatctg
    25081 tatcgtcttt ttctgcaggg gaacgggtat actctttggc ttttgcaaat acttccttct
    25141 gctcttcgtt cgacaatgaa tcaaaatcgc ccactgccag atctactggg tagcctgctt
    25201 gaagcaggta cagcgcacca cggtctatcc cgacataggc atcgtatttg gacagcagag
    25261 actgcggcca gttttgcgga ttacctccag cagctaataa aattttcatt tagtctaatg
    25321 cctcttttaa tcgttcgatt tgtccgactg gattttcggc tccatagata tatgatcctg
    25381 caacgaagac atccgcccct gcatctttac agatttttgc tgtttctgga acgattccgc
    25441 catctacttc gattgcataa gtccaattgt tctctctacg tagatcagcg agttccgtaa
    25501 ttttttctac tgtttcttca ataaagcttt gtccgccgaa cccaggattg acagtcatga
    25561 ctagtacttg atcagccaaa ggcaaaacat gtttgatcat ggagactggt gttcctggat
    25621 tgataactac tccagcttta actcctaagc tttttgtcat ttgaagtgcc cgatgaatat
    25681 gaggagttgc ttcgacgtgg acagtaatga tatctgctcc agcttttgca aatgctggaa
    25741 tgtagttttc tggatcaacg atcatcagat ggacgtctaa cggtaattta gtaacaggac
    25801 ggattgcctg aacaatattt ggccctaatg tgatatttgg gacaaattgg ccatccatta
    25861 catctacgtg gatgtaatct gcgcctcctt tttccactaa ctcaatgtct ctttgtaaat
    25921 tggcaaaatc tgcactcaaa atggatggtg ctatcttcat tcttgtttcc tcctattttc
    25981 gtttaccttc tttttttgca tacattggtt ttcttttttc gatttcttgt aagaattgca
    26041 aataattgtc gtaacgtgtc tgtgcgatac cacctgtttc tactctatgt tttacttcgc
    26101 agccaggttc tttagcatgc atgcactctc taaatttgca atggacagaa gcttctacaa
    26161 attcaggaaa ttgtctcggc agttctcctg cttcaattgt caaaaagtca atcgagctaa
    26221 atcctggtgt atctgctact aacccgccat agataggaat cagttccaca tgacgtgttg
    26281 tatgccgtcc tcttccaagt gcatcagaaa tctctcctgt tgctaaattc agttccggtg
    26341 atatttgatt cagtagcgtc gattttcctg ctccagactg acccataaag accgatagcc
    26401 gttctgggaa gtaacggatc agttctgtcg tatcacccgt atgattgggt acaatcaccg
    26461 gatagcctat tttagtatag gtttgcttga tctcttcgat tttttctggg tcgtctgaca
    26521 agtcggtctt tgtcaaaaaa ataatcggtt cgatcgactc gtattccaag gtaactagaa
    26581 agcggtcaag tagattataa gaaaatgcag gctctacgat acttgtgaca atgattccaa
    26641 gatcaacatt tgtgactggt gggcgaacta attgattttt tctcggcaag atttccagaa
    26701 gatagccgtc tgtttgattt tcactctcaa agatcacctg atctccgaca agcggcgtga
    26761 ttttccgatt ccggaaattt cctcttcctc ttgtttgata ggtctctccg tttgcgtata
    26821 catagtaaaa accgctgatt gcttttctga tttgtccttt gataagtgcc acctccgttt
    26881 ttgtttattt taccatacga agaggaaagt aggtgttatt ccatgcaaac ttctcaaagt
    26941 atatggcttg aaaattgatc aaaagagata gaaacacaaa aaagagaatg caacaaatca
    27001 atgtcgcatt cccttctgat atattttatt cagcacttat tttgaacttt ctgaggtgct
    27061 agctgatgaa cttgtggagc tggaagattc ttcttttgaa gagttgcttg tgctactgga
    27121 gctacttgag ctgctagaac tgtctgaaga gtctttttca gaactagaag acgctggatc
    27181 tggtccttta gagtaaatta cgttcacaac cattcctttg gtaattgtcg ttcctgcccc
    27241 tggtactgta cgaatcactt tatctttatc caccgtgttt gaatattcgg aagtctcatg
    27301 cccgatatat tctgcaccga cactagctaa ataactttgt gcttcgcttt ttgtatagcc
    27361 ggagatatca ttcaatttag gctctgttcc ttggcttacg acaaaattga tgtaagtttc
    27421 ttctgctaca acttcacccg aggctggatc ctgactgatg atcgaacctg atggtacagt
    27481 gtcgctatag tcataagtag cactaatatt attgatattg aatccttgtg taacaagcca
    27541 attacgaaca gtttcgtagt tttctcctac atgactactc aaagtcactg tttctggtcc
    27601 tttgctgact gtgaaagtaa tcgtgtctgt tttaggatcg acttctgtat cttctgatgg
    27661 agattgcgaa atgatcattc cgctttctac ctctgaactg aattcctctt ctttttgaat
    27721 caagtcagca gaaaacccta attctttcaa acttgcttcg gcatcttcat aacttttacc
    27781 actgtaatca tctaatttga tttttttcgt gccgctacta atatacagag tgacttcgcg
    27841 tttttgcttg actgtcgttc ccgctgccgg gtctgtcttc actacattac cttcttcgat
    27901 tttgtcatct gcaatttctt ctgtttttgt tgctacttct aatttcgctt ttgcgagtgt
    27961 ttctctagca gaagcttcag aaagatcaga aacatcagga acaacaactt ctcctcgccc
    28021 gccagaagca aagaatacag ctgttccgat tcctaaaaca gcaagaatag ctaataggat
    28081 cagccaccat tttttctgtc ctttcttttt aggatcaggc agaacttctt cctcctctgg
    28141 caaggtttcg ttttctggct tgtcttttgg cagaggcata gatttgaatg aatcaggcat
    28201 tggtgcatct tccgtaaggg gtgtgataac ttttgtttca ttatccatca catgaggctg
    28261 ccatttttct tcgtttgcac gagctggtga taatactgta gaaagatcat cagacatttc
    28321 ctcggctgac ttataacggt ctgcaggttc tttagccgtt gcccgtaaaa tcacgttttc
    28381 cagtgcttgt ggcacatttg gatcaagtgc ttttatagat ggaagatcat cttgaaaatg
    28441 ttttaaagca attgttacag cagattcacc atcaaacggt acgctacccg tcaacatctc
    28501 ataaaggatg atccctaaag cataaatatc tgactgcttc gttgccatac ttcctctggc
    28561 ctgctcaggg gacagataat gaacagatcc cagcatcgta ttcgtctgcg tgatcgatgt
    28621 ttcagataaa gcgatagcaa tcccgaaatc cgtgattttt acaacacctt cattatcgat
    28681 cagtacgttt tggggtttca aatctcggtg gatgatttga tggttatgag ctaaagaaat
    28741 agcagaaagg atctgctgca taatatttac tgctgtttca taaggaacag gataatgtgt
    28801 ttggatatat cgtttcaagt ccattccttt gacgtattcc atcaccagat actgcatgtt
    28861 gtcttcttca ccaacatcgt aaacactaac aatgtttgga tgaacgagtt ctgttgctgc
    28921 taaagcttct ctctgaaatc gtcgaatcgc agtctggtca ttttgaaagt caaatcgtaa
    28981 gactttaact gctacatcac gatccaagat taaatcgtgc gccaaaaata catttgccat
    29041 accaccgcta ccgatatttc cagtgatttg ataacggcca tttaattttt tgcccaactc
    29101 gatcatacac tgtcctcctt atattcgata agaagaacgg tgatattatc cgctccgcct
    29161 gcttcattgg cacgatcaat cagctcagtt actttatgag acaatgggcc ttcttgattg
    29221 atgatcgttc ctatttcttc ttcagacagc atgttcgtca agccatctga acataacagc
    29281 agtcgatctt ttagttgcca aagatgagta gaaacatcta cttcgactgt tccaggcatg
    29341 ccgacagaac gtgttaaaat atttttcctt ggatggttca ctgccatttc ttcgctgatt
    29401 tctcctgatt tgactagttc attgactaaa gaatgatcct ccgtcaattg catgatttta
    29461 tcttcacgaa tcaaataagc tctgctatcc ccaacattgg caatcgtgaa ccagtcatct
    29521 gacagagcgg ccgctacgat tgttgttccc ataccgttat attccggctg ttcttgtcca
    29581 cgctgataaa tcagcatatt ttcttcttga atcgattgaa tgaaccattg ggcgattttt
    29641 tcatcgtcaa cagcttcttg tttttcccaa gcttcgccta gattcgttac cgccatctga
    29701 ctggcaatgt ctccggcacg atgaccaccc atcccgtctg ctaatacagc aagcttgaga
    29761 cctgaacggc tggtaaatac actggcatag tcttgatttg tgttacgtcg tcttcctaca
    29821 tctgattgat attcaatctg cattttttca cctcattact ttttgcgcaa acaacaaatg
    29881 aaaaatccat ctgtgtaaaa ctgatgcgga tacaaagtca acattttctc ttcgattgat
    29941 gattgtagca taggattggt caaaacatca atcctctcaa aatcagggtg tctttccaag
    30001 aaagcagaaa caacgtcttg gttttcttct tttaagatcg tacaagtgct ataagtcaga
    30061 ataccggaag actttaaagt tggtgcgcag ctttctaaaa tttccaattg gatttttggt
    30121 aaattcgcta attcatccgg ctgtttttta taacgaatat ctggttttcg ccgaagcagc
    30181 ccaagacctg aacaaggcgc gtctactaaa atccgatcaa actgctctgc ttcgaactgt
    30241 ttactgactt ctctagcatc catcttttga gcgtagacag cagaagagac acctagacgt
    30301 gctgcattat cttcaatgag tttgactttg tgttcgtgta catctaaagc gaccacttct
    30361 ccaccgcatg acggatctaa aaaagaagca atatgcgtcg tcttacctcc gggtgctgca
    30421 caggcatcta agacatgatg ttcaggctcg atttgcatag acggcgctac aagcatcgaa
    30481 ctttcatctt ggatcgtcat gacgccatcg tggaacaatg agctagaagc taaatgccct
    30541 tttttcgcta ctataccgta tggactgact tggctttttt cagctgtgat ctcttcttct
    30601 tgcaaaacag tgatggcctg atctctggaa agtcggcgtg tatctactcg ggcactagca
    30661 tggctaggct gatatagcga taaaccgagc ttttcagttt cttcgtatcc gatttgatcc
    30721 accagtcgtt tcgtcatcca tagaggcagg ctgattttga cagaaagtct ttcgatagga
    30781 tcagcaatgc cttgcagatc tggtgctcct tgacgctgat aattcctcaa gacaccattt
    30841 acaaatttcc ctgtcccagg atttcctttt acttttgcga tttcgaccgc ctcgtttaaa
    30901 atagcatgtg cagggacttt atctaaaaat tctaattggt atacagaaag ataaagaagc
    30961 gtcttgaccc agtcatccac tttttttgct ttagcgatca atggtgcaac gtaaaattcc
    31021 aatagtcgct gatggctaat cgtaccatag atcaattctg taaataaacc cacatctttt
    31081 tgatttagct cgctttgttt gatcatctca ttcaacaaaa gattcgagta ggcgccaccc
    31141 ttgttcactc gctctaatgt cagtaatgcc atatatcgta ctgatttttt cattttttta
    31201 gggatttttt tcattatttc acctgctgac caatctgaac ttgctgaccg cttccgttaa
    31261 ggaaatcatg tacggcctgt tttcctttac cagctggctg taattcattg atggctaaga
    31321 cggtctgttt accgcaagca atccatagat tttttttgtc tttccggatg atcgtaccag
    31381 gtgctttgtc tgtagtttct gccaatactt caaccgataa taatttccag cgtatgccct
    31441 cataggtcgt gaatgcaatt ggccaaggac gcattccgcg tacttggttg tcaatttctt
    31501 ctgcagtttt atgccaatcg attgcttctt gttcccgtgt gatgtttggc gaaaaagttg
    31561 cttctgactc gtcttgaggt actggttttt gtccatccaa aatattgggc aatgtttcta
    31621 gcaatagctt tcttcctaaa aggctaagct tttcaaacat cgtccctaca tcatcttgtt
    31681 tagtgattgg tatgctttct tgcgcataga ttccgcctgc atccattttt ttgatcattt
    31741 ccatgatcgt tacaccagtt tctttctccc cattcatgat ggcataatga acaggcgcac
    31801 ctccccgata tttaggaagt aaagaagcat ggacattgat tgcccctaat tttggtactt
    31861 gcaataactt ttcaggcaag aattggccaa atgccgcagt gacgatcaca tcaggcacca
    31921 attcaatgat ttcttccatt tctggcgaac cggagatttt ctctggctgc agcacacgga
    31981 taccgtgctt cagagccgct tcttttaccg gtgtcggcgt aattactcgt ttccgcccaa
    32041 ctggacggtc gggctgtgtt accactgcct ggatctcgta acctgattct atcagccctt
    32101 caagaatcgg cacagaaaaa gcaggtgtgc ccataaatac tagcttagtc atcaagatgt
    32161 tcctccatat attcttcaag ttcttcttcc gggatctgct gtatcatttt ctcaatgaac
    32221 aagataccgt ctaagtgatc gatttcatgc tggaaggcac gggctaaata gccaaaagcc
    32281 gtcacttcta tctcttctcc gtctcgatca taataacgca cggtcacttc atctgctcgc
    32341 ttgaccgttc catagacatg cggaatactg agacaacctt ctacgtcaag gctttcacct
    32401 tttgcttcga tgatttctgg gttaatcagt tcaaatttct cgccttcatc tacttcgatc
    32461 acagcaattc tcttgttttg tcctacttga ggagcagcga tccctattcc gtcattcgca
    32521 atcattgtct catataaatt gtctaataaa gcgattgtct cgtccgtgat tacatcgatt
    32581 ggctgagcag ttctttttaa tttatcatta gggtggatca atataggata gcgcataatt
    32641 tggccctttc ttaaataaag tttaatggtt cgttgtcaat tgccacaaaa agaccttgtc
    32701 gctgttcttt ctggcttcca ttcaaaattt catccaaaac atgaggaaga ttcggttcat
    32761 gtttatattt gatgattagt tgataataat atcggttttt cactctcaaa atcatgctgg
    32821 gcgttggccc taatagcaag ctttgagggc ttaacacttc ttttagctga ttggcaatct
    32881 gaaagatttt ctttgccgct acttgttctt caggatggct ggcggttatt ttcacagtaa
    32941 agtaataagg aggatagcct ccacgatgcc gtacatgcat ttcctgctga taaaactgct
    33001 cataattttg ttttttagct aattctatgg cgtaatgttg aggattgaat gtttggatca
    33061 cgacttcgcc tgctttttcc gctcgccctg ctcgtcctga aacttgggta agcaattgga
    33121 atgtatgctc actagaacga aaatccggta aattcaggga ggtatcagca ttcaacaccc
    33181 caactaatgt cacttctgga aagtccaaac ctttagcaat catttgtgtc cctaataata
    33241 tatctgcttc ttttgcaccg aatttttgta aaatctgttc gtgtgcccct tttcttcgag
    33301 tggtatctac atccatacgc aaaatcctag cttgcggata aagttctcgg agttcctctt
    33361 ctactttttg agtacctgtt ccatagtagc ggattttatt gccgccacaa ttaggacagc
    33421 gattgggaat aggttcttca tgtccgcaat aatggcagcg cattgaacgt gtatccatat
    33481 gcagtgtaag agagatatcg caattaggac atggaagaac aaatccacag tccctacaca
    33541 tcacaaaaga cgaatatcct cgacgattga gcagtaggac agtttgttct tttttgtcta
    33601 atcgattttg tattttttcc tgcaaattag cagaaaaagt agaggtccga tgattctgaa
    33661 actcttcacg catatctacg atctcaatag ctggaagctg tgcagctgct ttggctcggt
    33721 ggtttaactg caataactga taaacgcctt tttgagctcg tgctcttgat tctaaagagg
    33781 gcgtcgcgct tcctagaact attgggcaat gatgatattt actgcgccaa attgctagat
    33841 ctctcgcatg ataacgcggt gtctcatctt gtttgtaact tgattcatgt tcttcatcga
    33901 taatgatcag cccgatattt ttcagcgggg caaaaatagc agatcgtgcg cctactacta
    33961 cttgcgcttc ttcccgctca atcttgcgcc attcatcata tttttcacct tgggaaagtc
    34021 cgctatgtaa gaccgcaact gattctccga agcgtccttt gaatcgctcg accatttgcg
    34081 gagtcaatgc gatttcaggc actaacataa tagccgtttt attttctgac aaaacatcag
    34141 cgattgcctg aagatatact tctgttttcc cgctgccagt gattccctcc agcaaaaaga
    34201 cctgatcttt ttgctgctga cctgctgtga tgatttgttc gacagcattt ttttgttctg
    34261 cattcagttc caatgccgtc gtttggtcaa atactcggtc tttataaggg tcgcgataac
    34321 gttctgattc tataaactcc agccagccct tttcttttcc ttgattcaag acagcagtac
    34381 tgattccttt atttttgaaa taagcaattg gttcttcttg gctcagttca gataaacaca
    34441 aaagaagttc tttttgtttc ttggcatttt gccgtaattt tgcccattct tcttcgattt
    34501 gcttttcagt taacaaagaa cggaccaaac gaatcgtttt tactttattt cgtgtatgga
    34561 cttcatattt gatatctact aattgctctt ggcgccagcg aatcaattga gaaaggctgc
    34621 cttcttcttc tgcttccttc caggaaatca cttctcgctg atcaaacaat acctgaactt
    34681 ctggtgtgtc tgccaaagga tagatcaatt tatcatattc tgctttcatc acactcggaa
    34741 gcatcgtttg tagacaagtg attttgaatg cgtaagtaat ttttttcata taatcagcta
    34801 atgaaagtaa ttcttcattg accaccggag caaggtctag caaacgaata actgccttca
    34861 aagatggatt agctgattct tctgattttt ggatagccat aacaaatcct tggacatgac
    34921 gattgccatt accaaaaggg acttctactc gcatcccgac ttgcaaagct gtttccattt
    34981 cagagggaac caaataagtg aatggctgat cggtctgcat agtaggtacg tccacaatga
    35041 cttcagctgt taatgtcatg gttcgtttcc tccttttttt gtcttttctt atcaagcgac
    35101 aaaaagttcc ttgcctattt tactagagat cgatggattt gtcttatctt ctgccaaatc
    35161 ctaacaaaaa attaacagca gataagaaaa aaaagaactg tgtcaaaaag ttcagacaca
    35221 gccttttcta ccagacaata agtggcgaat gtttgaaaag gaaacagctg ggtttgcttt
    35281 tctttttaga tgttcgctct atttttcttt attacatttt ttgctcttca cggatacgtg
    35341 attccaattc gtgttgttca cgctcttttt gtgcttgacg ttcctcttct tccatcttca
    35401 aacgttcacg ttttaattca ggatgtggat cattgactac atttcctgca tcgatttctt
    35461 ctaaagcttg accgacgctt tttactgaat caaaagaatc tagagtaggc tgtgcacttg
    35521 catccaattc atgcgcgcgt ttacttgcta aaatcactag agaatatttt gaatttacac
    35581 gatctaatag tgagtcaatt gaaggtttta acatcataag actacatctc ctttaacatt
    35641 ttaatatatt taccgattac gcgatctact cgaaaatgtt cactggcaat aatgtttttg
    35701 attcgttcaa ctgctagcgg cacttcatca tttacaacag catagtcgta aagagccatc
    35761 atttcgattt cttcttttgc aactttcatt cgttcttcga ttacttcatc tgcatctgtt
    35821 cctcggccta cgatccgtga ttttaattcc gctaaatctg gcggagtcaa aaagatgaat
    35881 acaccatcgg gcactttttc tttgacttgt tttgctcctt ggacttcaat ctccaagaaa
    35941 acatcttttc cttcatccaa tgtcttgttg atatatgaca aaggagttcc ataataattc
    36001 ccgacatatt ctgcgtattc cagcatctct ccagcttcga tcattgattc aaactcttct
    36061 ttgcttcgga agaaataatc aacgccttcg acctcccctt cacgcatttt acgggtagtc
    36121 attgagatcg aatactgaaa atcattatcc tcactctcaa aaattgcttt tcgtactgtg
    36181 ccttttccta caccggatgg accagacaac acgattaata atccacgctc tgacatgacg
    36241 acgtcctttc aactagtctt tttgtactta tcgtttattt tgcacttttt ttctgcaact
    36301 ttcaaggtta tctttaatta tagctagaaa ttggtaaaaa agctaccctg taccaagagg
    36361 tcgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacataaac agcttctcat
    36421 gtttttcgtt tggttggggt tattatcgca gatgcaagct tttgaacgcc gtgtataaga
    36481 ggtcgtgaag aagccgatta gtttcgagta tgaagaaaaa ttttgtgaaa atagtttctc
    36541 acatttttgc ataaatttgg cttaata
//
LOCUS       DAFCNS010000023        34770 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFCNS010000023 DAFCNS010000000
VERSION     DAFCNS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.023"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(226..1113)
                     /locus_tag="LNE23_001071"
     CDS             complement(226..1113)
                     /locus_tag="LNE23_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6930942.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1110..1979)
                     /locus_tag="LNE23_001072"
     CDS             complement(1110..1979)
                     /locus_tag="LNE23_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6930943.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2116..3402)
                     /locus_tag="LNE23_001073"
     CDS             complement(2116..3402)
                     /locus_tag="LNE23_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6930944.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3675..4253
                     /locus_tag="LNE23_001074"
     CDS             3675..4253
                     /locus_tag="LNE23_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK6930945.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4253..5182
                     /locus_tag="LNE23_001075"
     CDS             4253..5182
                     /locus_tag="LNE23_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6930946.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5179..6915
                     /locus_tag="LNE23_001076"
     CDS             5179..6915
                     /locus_tag="LNE23_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK6930947.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="LNE23_001077"
     CDS             complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="LNE23_001077"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBK6930948.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7811..8320)
                     /locus_tag="LNE23_001078"
     CDS             complement(7811..8320)
                     /locus_tag="LNE23_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBK6930949.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="LNE23_001079"
     CDS             complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="LNE23_001079"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK6930950.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9621..10712)
                     /locus_tag="LNE23_001080"
     CDS             complement(9621..10712)
                     /locus_tag="LNE23_001080"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBK6930951.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="LNE23_001081"
     CDS             complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="LNE23_001081"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBK6930952.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="LNE23_001082"
     CDS             complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="LNE23_001082"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBK6930953.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="LNE23_001083"
     CDS             complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="LNE23_001083"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBK6930954.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14675..15649)
                     /locus_tag="LNE23_001084"
     CDS             complement(14675..15649)
                     /locus_tag="LNE23_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBK6930955.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15630..17513)
                     /locus_tag="LNE23_001085"
     CDS             complement(15630..17513)
                     /locus_tag="LNE23_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBK6930956.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17531..19756)
                     /locus_tag="LNE23_001086"
     CDS             complement(17531..19756)
                     /locus_tag="LNE23_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK6930957.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19871..20905
                     /locus_tag="LNE23_001087"
     CDS             19871..20905
                     /locus_tag="LNE23_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6930958.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20972..21985)
                     /locus_tag="LNE23_001088"
     CDS             complement(20972..21985)
                     /locus_tag="LNE23_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6930959.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22063..22251)
                     /locus_tag="LNE23_001089"
     CDS             complement(22063..22251)
                     /locus_tag="LNE23_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBK6930960.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(22691..22819)
                     /locus_tag="LNE23_001090"
     CDS             complement(22691..22819)
                     /locus_tag="LNE23_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK6930961.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(22822..24195)
                     /gene="rlmD"
                     /locus_tag="LNE23_001091"
     CDS             complement(22822..24195)
                     /gene="rlmD"
                     /locus_tag="LNE23_001091"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK6930962.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(24306..25361)
                     /locus_tag="LNE23_001092"
     CDS             complement(24306..25361)
                     /locus_tag="LNE23_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK6930963.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(25371..26801)
                     /gene="gatB"
                     /locus_tag="LNE23_001093"
     CDS             complement(25371..26801)
                     /gene="gatB"
                     /locus_tag="LNE23_001093"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBK6930964.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(26798..28270)
                     /gene="gatA"
                     /locus_tag="LNE23_001094"
     CDS             complement(26798..28270)
                     /gene="gatA"
                     /locus_tag="LNE23_001094"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBK6930965.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(28270..28575)
                     /gene="gatC"
                     /locus_tag="LNE23_001095"
     CDS             complement(28270..28575)
                     /gene="gatC"
                     /locus_tag="LNE23_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBK6930966.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(28681..30717)
                     /gene="ligA"
                     /locus_tag="LNE23_001096"
     CDS             complement(28681..30717)
                     /gene="ligA"
                     /locus_tag="LNE23_001096"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBK6930967.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(30734..32968)
                     /gene="pcrA"
                     /locus_tag="LNE23_001097"
     CDS             complement(30734..32968)
                     /gene="pcrA"
                     /locus_tag="LNE23_001097"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBK6930968.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            33123..34682
                     /locus_tag="LNE23_001098"
     CDS             33123..34682
                     /locus_tag="LNE23_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBK6930969.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT         9518 a         7494 c         6335 g        11423 t
ORIGIN      
        1 acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt ttttagcatg
       61 tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg agcacaaact
      121 taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa aagtatcttt
      181 ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt ttacagagcc
      241 gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag ttggtaaagt
      301 agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag cacccatagc
      361 agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta agggccataa
      421 ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg tatttttagc
      481 cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta atcgagcggc
      541 ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga tcaaaaaagc
      601 agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa ccgttccgtt
      661 caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa ccatcatcga
      721 tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct caaatccata
      781 tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga tcatcgctga
      841 attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa aattgatgat
      901 caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat tcgtcattcc
      961 caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta atgctgtata
     1021 ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga cgttggggcg
     1081 ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac tttcgtttgg
     1141 attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc cgcatatcca
     1201 aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt ggtcgttgca
     1261 tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa tgtcccgatc
     1321 gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt cactttgata
     1381 aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc cgaatcaata
     1441 ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca agtgatcgaa
     1501 agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg tgtgtcaatc
     1561 ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag acttttcatg
     1621 atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt tcgaaacaac
     1681 cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc tagaaacaac
     1741 atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt tttaaaagtt
     1801 gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt gacaggggga
     1861 ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat gattgatatc
     1921 aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt ctgcttcatt
     1981 cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag acaatatctg
     2041 taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg aagaaattca
     2101 cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg tatctgctag
     2161 taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta cgactgcttc
     2221 aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc atgttgaaaa
     2281 atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag cttcttgttc
     2341 attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc ccatcagttc
     2401 ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc cagctactcc
     2461 tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt gcagattttc
     2521 tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg ctccttggat
     2581 cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt gtatcgcagt
     2641 tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa gagtggcaaa
     2701 caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct ccccatcttc
     2761 agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa ggtcagaagg
     2821 attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta cttgaatata
     2881 ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg ccatcagatc
     2941 cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata ttttatctcc
     3001 tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa tagcagacaa
     3061 attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat cttcaatcaa
     3121 gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca gtttctgaac
     3181 gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct cataaacttt
     3241 ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga cagtgacttt
     3301 ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac ctgatgctgc
     3361 cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc gctccttttc
     3421 catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa acggtttctt
     3481 tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa atcaactatg
     3541 acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt tctttaaaat
     3601 aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag actattctta
     3661 aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag acgattttag
     3721 atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata gggacttttt
     3781 ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc gtggtcacag
     3841 atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa cagcaacaac
     3901 ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt aaagattctt
     3961 ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat ttttttctaa
     4021 attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc ccaatcacca
     4081 atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa actcgtccat
     4141 tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg aaaaacggcc
     4201 attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat tgatggatag
     4261 cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg gctatgtaat
     4321 caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc aagaagtttt
     4381 tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca atgaaatatt
     4441 ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa agaaccttgc
     4501 ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa gtagttattt
     4561 aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga atttcttcac
     4621 gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa tcaatcgttt
     4681 ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac tttctttgct
     4741 caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt tttctattct
     4801 tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa aagacaattt
     4861 cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag aaatcaattt
     4921 acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca tctttactga
     4981 aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta aagccaagct
     5041 ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg gctataccga
     5101 actgggttat ttctccagag tttttaaaga agagacagga atgacacctt ctcaataccg
     5161 taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt tgccaaactt
     5221 ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt gtttatttat
     5281 catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt ggtactaaaa
     5341 aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt agccgctttt
     5401 gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca tatggaacag
     5461 cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat ccatccaatg
     5521 atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg aaacgacatg
     5581 ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag agtgaatgaa
     5641 cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg cgataagaaa
     5701 gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt ttacggatat
     5761 gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt agtaaatcct
     5821 gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc aaatgaagaa
     5881 aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga aacaacgatt
     5941 tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac attactcagt
     6001 caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat tattttgtat
     6061 aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga tcgacacaca
     6121 aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg cttaaaagct
     6181 actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat caatcaattg
     6241 ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa gaaacgttcg
     6301 cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa tacgctgact
     6361 agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg aatcgacagt
     6421 ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc agtcaaggaa
     6481 gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg cgaaagaatc
     6541 tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa tatgatcctt
     6601 caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca aggattcatc
     6661 acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga taacggcgat
     6721 ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat cggcatcagc
     6781 catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac gatccaatca
     6841 gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa aaatcaatca
     6901 gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt aaataaatca
     6961 atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg ataacagccc
     7021 aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc tactaatgtc
     7081 attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct agttttcaat
     7141 ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc cagcatttta
     7201 tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt tactggagct
     7261 gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg gttcaactca
     7321 ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt ttctaatgaa
     7381 gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc agcagttgtt
     7441 gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg ttgtgattgt
     7501 gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt gcctaaaagc
     7561 tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg tttggaaaga
     7621 agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac gacagccaga
     7681 tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata cgcagtcaat
     7741 tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc tggccctaga
     7801 tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc ttttctggtg
     7861 tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa agtcgttgtg
     7921 ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga atagacgttt
     7981 catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa agaacaatcg
     8041 gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca ccagtcgcga
     8101 taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt cgaaagcttt
     8161 tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc tcaatctttg
     8221 tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg attgttgttt
     8281 ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc tcctgacaca
     8341 agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc agctccttct
     8401 agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc gattcgatga
     8461 tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat gatcagaccg
     8521 tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac cgcatcaatt
     8581 ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat tccttctgct
     8641 tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat gatttctcca
     8701 ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga ttggttctgt
     8761 tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc taagatacca
     8821 gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg aacaagaagt
     8881 cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat ctgttgacca
     8941 gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat ccttccgcca
     9001 aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac gattacggat
     9061 gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc actggctaca
     9121 ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat tggtagatac
     9181 tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc catcactcgc
     9241 cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa aggctgtcca
     9301 gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc aattgattcg
     9361 gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc ttcctcttct
     9421 tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc ttctgcgcgc
     9481 aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat gctgcgatgg
     9541 gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat ttgttgtaat
     9601 aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt agacataatg
     9661 attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag tattcctaaa
     9721 atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc ttttccgatc
     9781 agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa taaatcaaaa
     9841 aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc ttttgcccga
     9901 ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc tatttgtgtc
     9961 atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct caacaaaata
    10021 tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa gcctcctgct
    10081 gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt gactaagcca
    10141 gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg ttctcttgct
    10201 gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt ttcaacatct
    10261 tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag tttagcatct
    10321 gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac aaaccgaaga
    10381 tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc cgtaatgatg
    10441 acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt tgtacggacg
    10501 ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc acttgcaacg
    10561 taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact atcccaacca
    10621 actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc accaatcagt
    10681 ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt agtaatgttt
    10741 caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat cacttgggaa
    10801 ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa cgacgctagc
    10861 tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt cttttgtatc
    10921 gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga ccccgcgaat
    10981 gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat tggtacgtcg
    11041 gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc ctggtaatga
    11101 accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac taaccacagc
    11161 ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta atccagctgg
    11221 aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt tctttgtctg
    11281 atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta cttctgacgc
    11341 ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg cttggattcc
    11401 accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa ttttcttggc
    11461 aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt caagcacatt
    11521 ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc ctggacgcgg
    11581 acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag acatgacagt
    11641 tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac caattgtttt
    11701 gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca tccttcctcc
    11761 tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt ctgacagagg
    11821 taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg attctcccgc
    11881 agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct gtgggaacag
    11941 gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg cttcctcttg
    12001 ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga tcgtacgtaa
    12061 tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca gttggagaca
    12121 agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact ccttctccat
    12181 gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta tgaccaaaat
    12241 ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt cgtttgattt
    12301 ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg tctttcagct
    12361 cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg atttcagcga
    12421 ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca atcaatacac
    12481 cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg actgctgtct
    12541 tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga acgaaatgga
    12601 tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt cctccaccaa
    12661 gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg tataactcag
    12721 ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg actggttcaa
    12781 aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca acgtttgtat
    12841 cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat ccagcctgtt
    12901 ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt acagtaagca
    12961 ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa tcttttccat
    13021 tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt cattcaacga
    13081 ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga tcatgccatc
    13141 tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa ttcctgtacc
    13201 atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg ggacagcact
    13261 taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt cgataaaaat
    13321 gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt cattgatcgt
    13381 accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag cagatagcag
    13441 tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact cagccaccat
    13501 atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt catcagccgc
    13561 ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg gagaagtacg
    13621 tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca ttcgcgcaca
    13681 ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca cgaaactgcc
    13741 gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga attctcgaga
    13801 agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat agcgtgtaaa
    13861 tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc gttctgttcc
    13921 ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt tttttatttg
    13981 ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt tcttttgttt
    14041 acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat taattcctac
    14101 taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta tgacccagat
    14161 aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat cttcaacgag
    14221 ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct ctacgtggct
    14281 gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg tttgtaccca
    14341 cgaagtcatg aatacaaacc tatctaaaat agctaaagta atacgtattc agttttgtaa
    14401 gttttctaga tttcataaaa aatcgctcca aactgatgta agaattgtac ctagtataga
    14461 cataaaaaaa tcgactgtca atcattttta tgaaactttt tttaattttc tgatagttaa
    14521 ctttgttttt cagaaaaata acaattggtt ttattgaaga aaatagtcat gaacaaaagt
    14581 atctgagaac ttttttactc tcttgttcat gacatataga ataaagcagc tttgtcttgt
    14641 aaactccgtt tttcaaataa taatctaaag attgctactg aataataaag ccgatttcat
    14701 ggtcctcttc gctgctgatt cggtaaggtg actcaacatc cgctccccag ctattgatac
    14761 ccccgactcc tcttactttt gcaaaaatcg taagaaccgt tcgtctttct ggcggtagtt
    14821 cttctatatg tgttgcctgt tccagttctt ctgctttgaa tggtaaagcg gaaaaagcaa
    14881 atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc ttgatcttta tccgtattat
    14941 ccaacgtttg atttcttgtt agttctaccc atttcgtttc catatgcatg ccgcattctt
    15001 ggggaacaag gtatggggaa acggggagtc ctgccacttt ataaatacct tcgacaccac
    15061 cagccatccg atccggatat gtttcccctg ataggccttc atactcaaaa aattttgctt
    15121 tcgttggcat aatgaacctt aaacccaatg cgggtaattc cggtaatcct ttttgtccat
    15181 aataatgcat attgacaagc attttaccct ctccagcgat ttggtaggaa attgtcactt
    15241 gggtttttgg tactgtgttg atttccagtg taaaaatgat ttcaaccgtt tcagcgtatt
    15301 cattacttga atattgatta tttactggag caatcggcaa atcgatcttt ttattgtcca
    15361 ccagtacttc tatttctttg acttttggat acaatccggc tcctaaccac ataccggatt
    15421 taaatccaaa tccgctacct ctgtcattat cggtcgttgc tcgccaaaaa actggaagtg
    15481 tttcccggta tagccattct tttccgtttt tattgagcga ttccagtccg cctcttgtat
    15541 aagaaaaaat ataatgcgta ttccccgatt tgacacctaa tgtacagtcg ccgaagacga
    15601 ttgcaacttt ttccgtatcc atatgacttt tatttactgt atttttcata ataatatctt
    15661 acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa cgatcccatt ccctgagaat
    15721 tcatagtcac ttggtttatc gtcaaagtca ccgccatagc gcatcacttc tctgcctgtg
    15781 attggatcag tcacttggat cgcctgatct ttaaaatccc atatatagcc tccttggtac
    15841 atttcataac gatcaagcaa cgtcatgtaa gaatccattc cacctaacga attccccatg
    15901 tcatgcatat attcacataa gatgaacggt ttttccgctt tattttcaag gtagtcaatg
    15961 atctcttctg gtggcgcata catgcagctt tttacatctg aaatcgaatc ttcgaatgct
    16021 ggatttggaa aattcccttc ataatggaca agtcgatcgg ggtctacttt tttgaagtaa
    16081 tcgttcattt gtcgaatgtt ttcttccgca taagattcat ttccaagaga ccaaaaaaga
    16141 atacttggat ggttttttaa tgtttcgaaa ttcgtttttg ctcgatcaag aacagccgcc
    16201 tgccactgag gtactgcacc tggtacgttg taagaaggct caaatgcgcc cattttttgc
    16261 catgagccat gggattcgag gttcgtttcc gccatcacat ggattcctgc ttggtcgcat
    16321 aaataatacc aagtgatctg gttggggtaa tgagatgtcc gtacgccgtt gatctttgca
    16381 ttttttatga tagccaaatc tttttccata tcttctatag tgatggcgcg tccttttttc
    16441 ggatgccact catgtcgatt taccccgttg ataatcatcc gttttccatt gaagtagatg
    16501 attttatctt tgatttcgat tttacgaaaa ccgaaggaat agtcaatata ctccgttact
    16561 cttttttctt cgtctatcgt ctccaattca agcttatata aattgggctg aaaatgcccc
    16621 cacagtgcca cattttcaaa tccatcgatg cgaatcgtca tttgctcttt taacggatat
    16681 tgctgttgat agatttcctt cccatcagga tctagtatcg ctacattcag ctgactgttt
    16741 ttggctactt gctcaaaaga caaatctact tcaagtttgc cattttgaca tttgtcatct
    16801 aagcgaggtt tgatccaaag atcttctaaa tggttttgtc ctcgagcaac taagaagaca
    16861 tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt ccaagaatgc agctgtactt
    16921 cttttgtaaa cctccacagc tagaatattt tcttcttcca aataaggtgt taaatcaaat
    16981 tcagacggag tgaacgaatc ttcggcatac ccgataaaat gaccattcaa ccaaataaac
    17041 attgcttgtt ctacaccagc aaaatgaatc gagatttgtt ttccctttag accttcttct
    17101 aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt tagcagctga aaagatccct
    17161 tccgcctctt tctcagggct tgtcgtgaaa gggggacggc gaaatacatg tccctcccac
    17221 ggatacatcg tattgatgta atggatcttg tcataatttg ctaattcaat atgactagga
    17281 acagtgattt catcaaaaga acaggcatca aaatcttttt tataaaatgt ttttggtcgt
    17341 accgaaggat ttggcgaata acaaaaacgc catgatccat ttaaagactg tttaagggag
    17401 gatgtggttt ctacttcttc ccttgttgca taacaaacat gatcgctatg tgcatttgat
    17461 tggttcactc taaaaacttg tggatcatct aaccatttta tatttgcttt catacttttt
    17521 tccttctctc tcataacttt ttgaaatgaa gcagttctgt ttggaaatca ccttgaaaat
    17581 cggggaagta aagtccaact gtcatcaatt cggcccccga aaatatccgc ttcgaggtct
    17641 gttcctgata aagtgcctca tcatctaacc ccatcagata tacttcatgg aacgctggtt
    17701 gagctgaagc tagtatcctc cctaaaaaca caatagcttc atcttgttta ggagacacaa
    17761 acatccatgc ggcaatattt tcttcaaaag gactcgccaa acgataataa tggccatact
    17821 ggatcaccgg acgaatcgtt tgataggata taatttggtc ttttacttgc tttttttctt
    17881 caggagacag ctttgtcaaa tccagttcat aaccaaaaac agcgctcata gctgtttctc
    17941 ctcttgtttt caacgaagta gatctgtcgg tctgatgatt tggtacagct gatacatgag
    18001 ctgtgatgga cgaaatcgga taagcaaggc ttgttccata ttggattttc atacgctcca
    18061 ctgcatccgt gttatcactt gtccatgact gcggcatata ataaataaac ccggcatcaa
    18121 accgtccacc tccaccagaa cagccttccc ataagatttc cggataatca gttgtcaaat
    18181 gttcaagtag ttcataaagc ccgagcatat aacgatgcgc tacttctcct tgtctctgtg
    18241 ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt catatcccat ttgatgtagt
    18301 caagaggaat cgtatctaaa atctttctca tttgtttctc aatcgtctgc cgaacatcta
    18361 accttgtaaa atccagtaca tgttgtgatc tggaagcaga aggcattcta cctggctcct
    18421 gcataagaaa atcaggatgt gttcgataca gttcagaatc aaccgagatc atttcaggtt
    18481 caaaccataa accaaatttc aatcctttgc tatgagcata atctgctatt tcacgtaatc
    18541 catttgtcaa cttgccttca tactcgaacc agtctcctaa tgaagaattg tccgcatctc
    18601 tttttccaaa ccagccatca tctaatacga acatttcgat gcctagctcc tttgcttcat
    18661 caatgatcgc cttgattttt tcactattga agtcaaagta agtggcttcc cagttattga
    18721 ccagaatcgg ccgttcagca aattgatgac gacctcttgc gattctttca cgtagcaact
    18781 catggtggat ctgactcatt ttgttcaatc cctgacttga gtaagaaagg atcatttcag
    18841 gtgtttgaaa agactggcct gcattcaatt cccacgaaaa acgatggctg ttgatcccac
    18901 cgacgactct caattgattg atctgatctt tttcaagttc gaaagaatgg ttgccggaat
    18961 aaacaaattg aagtccgatc gcttctccag tattttctgt cgtatgatga tgaactaaag
    19021 caataaagtt attcatatgg tgactgctcg ttcctcgacg gctttcaaat acttttgttc
    19081 cagataaaac agattgccgt gaaatctgtc tttcattgac atgtgctcct ggcaaagcaa
    19141 tgacttcatc aaatcgccta gtatgggcaa agtctagctg gaaactagcc gctttttcta
    19201 gtttgattgt ttctccggtt tcatttctta tctttacaga tcgtgtgatc actgctctat
    19261 cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt ttctctatcc ttaagtgaaa
    19321 tcaccaacgt ttgtgcctct gacttgtcca tcacataagt ttgaggcaag ccttcgatct
    19381 ctggcttccc atctgtaata taatggctgt catatctcag atccagtaag tctgatccat
    19441 tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat gctgccatga gaagagtatt
    19501 cttgcggtag tgtatctttc gacagagacc ggtcaggatt taagggaaca tttcctgaaa
    19561 atccacgatc tcttcgggga taatttttat tgtctttgta ctgcttgact ggtttgccaa
    19621 aataaatatg cgccaataca ttgctttctt ctatctttat cagataagaa agaaatgtat
    19681 ttcgtaaatg gaagactcgg ctcttttcat caaataagat gaaattttta tctgtttttc
    19741 tatgcgtaat atccatcatt tcactcccaa caaaacggaa accgtttact tttttttata
    19801 attcctgtat actgtttact aaacaatttt gtcaataaaa attttttatt tttttgagga
    19861 gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag gcaaatgtct ctcctgctac
    19921 agtttcccgt gtgttgaata acgatcagac gatccatgtg gctcagacta ctcggcaacg
    19981 gatagtagag gcagctgctg cgttggatta tgacatcagt actcgtaaat atatcaaaaa
    20041 gagaatgcct tcgattggtg tgatctctac gatcggcaaa gcgagcgaag aacaggatat
    20101 ctattacaaa catttgcgag tagggatgga agaagaagct cgacgtctgc atttagggat
    20161 gaatcgaatc tataatctat ctgataatcc taaacaatgg gaagatctag atcagttagg
    20221 tgcggtgatc gttgttggga ctgttacgaa agaatcgatt accgccttgt taaaacaaaa
    20281 catgcaaata gtcgtcgtgg ataaccctga catccagcaa gaggtcgata tggtgtatgc
    20341 agatcttgaa cggatgacta aaaccgtact ggaattgttc ttagaaaatg gtcataaaag
    20401 aatagcctat atcgggggat ataaagtcga tatggatgaa ttcggtggga agcagatttc
    20461 tataaatgaa aagaggatgc gtgcctatca gcattttatg atcgatcatc acttagaaaa
    20521 atatatgtct tatcatctag gtggctggac agaaaaacaa ggtgaggtac ttgcgactaa
    20581 tctattagaa aatgaatccc ctcttcctac tgccattctt gtcgggagcg atccaatggc
    20641 aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa attggagaag agattgttgt
    20701 agcaagcttt gataatatcg agacaatcgc aaacctctcc cctggcttga ccagtgttca
    20761 aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg gcattggagc gaattgacgg
    20821 tatacgggac gaaccaatca ttttgactta tcctgcacga ttgattgtta gggaaagttt
    20881 tatcccaaga acaaaaaggt attgaaagag aggttgtgaa aagccgcttc gtttcttcat
    20941 actcaaaacg aaacggcttt ttcacaacct cttacttata cttattcaca aaatccaatg
    21001 tctgctggta acaattttgc gattctttta gcgtgtaatc aaattggtat tcatgggtaa
    21061 tctctttttt tgtatctgta taaaacaagg attgcacggg tacctttaat tctgtcaacc
    21121 gattttctaa tgcttgacct tgctcctgga atgagtatgc atttccgtca gttatatacg
    21181 ttggcggaaa ttccttagaa acatggtcag ccacactagc ttctttcaat tgctcactcg
    21241 ttttccaatc ttttgttcca atcaacgacc acgcaacggt tttgacgaaa aacttcataa
    21301 atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc aacaggtccg caataagaaa
    21361 tggttccttt cagactatct tttggaagta gctgtttgat tttcatttgt ttagcatacg
    21421 catcattcgt ttgtatagct gcatattgca atgcgatttg tgaacctgca ctatctccac
    21481 caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt ttcttcagct aataaatctt
    21541 tgatcagttc attcacttga agaacttgat tagggtattc tgaatcaggc gctaattcat
    21601 aattcatcgc aaccactgcg atgttggcgt ctgaaactaa ttttgtcgca aattctttga
    21661 cacccgattt atctccgccg acgaatcctc caccatgtac ccaaaataga acaggaacag
    21721 gccccttgct gttttttgga tagtagatgt cataagtatt ctctttatac ttggactcat
    21781 acgtcaaatc tgtttttttc gccacgtttt tttctgcttg atcgaacgtt tttttatcag
    21841 tgatttgaac agtatttgaa aacatatggt tgatcaagaa tgctccaggt ctgggggaaa
    21901 gctggaaggc aagaaagatt gcaaacgcga tcccaagaat gattccgaag aaccatccga
    21961 taaatttaag aattttcttt ttcataaggc tccctcagta gttagtatta agcattctga
    22021 ttataccata gtgttatttc ctatcaagct ctcctagatg atttaatgat tttccccaaa
    22081 cttcttctgc aatgcttttg acaaaagaaa ttttttccat tgttcttcaa acaaaacatt
    22141 ttcttcttct gttgaagcaa atccacattg aggacttaag cacaaacgat ccaaaggcag
    22201 atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg tgctcttcca tttcgccatt
    22261 tttcgttaca gtcaaaatct aagacaacta atttatcttc agagactttc tctaaaagaa
    22321 caaaactccc agcacgttca ctatcgtatt ctaaataata ggcacggaca ttttcttgag
    22381 taaaataatg ggtctgctac gtcgtcatcc cccctgatgc cgccaaaatc gaataataat
    22441 ttccgcgtca aacatgtata tttagtgttg aatcatttga gaatatgaaa ataaaaatct
    22501 ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat ctgagacaga atgacgttca
    22561 ttctgaccca gatttgattt tgcacaactc aacagtcctt attggcaaat cgccaaaata
    22621 atgtagacta actaaaaaga ctaagaaaca ataacaaaag ttaacacttc ttagtctttc
    22681 tttagcacgt ctatttatta aagattttag ggccttttct catgttttta cgatcattct
    22741 taggtgtttc catttgtttg gatcggcctc caccttgttt ctttttgatc aattctttca
    22801 ttttttcttt ttggtccatt tttattctcc tttaacgaat gaagcgacta attcgatatg
    22861 atgggtttgc gggaaaagat ctacaggctg aactttcttc acggcataac ctgcttctgc
    22921 aaataactta gcatctcttg caaaagttgc cggattacat gagatataga cgattcgttc
    22981 tggtgacatt tcgattgctg attcaataaa atgatgatct aatccttttc gtggagggtc
    23041 aactacgatc acagatggtt tgatcccttt ttcttgccat tttttcataa ctttttctgc
    23101 ttttccgact tcataatgaa cattttcgat tttattattt aaagcattga actgtgcatc
    23161 ttcaattgct tctggaataa cttccatccc atacacctta tccactctgt ctgctaaaga
    23221 caagccaatc gtacctattc ctgaataagc gtcgatcaca atgtcttttt tatctaactc
    23281 ggcaaactcg atggctgttt ggtacagata ctctgcttgt tgagtattta cttgataaaa
    23341 tgactttgct gagattcgat aggtttttcc taatagctga tcttcgatat agtctctgcc
    23401 atataaaact ttttctttgt ctcctaaaat cacatttgtt ttttcttcat tgatattttg
    23461 gatgaccgaa accacttctg gcaattcttc atgaatagct tcagcaattt ccttggattt
    23521 aaagaatttt tctttacgag taactaaaac gaccatcatt tcatgggaat aatgaccacg
    23581 acggataatg atgtgacgaa gctgtccttc atttttttct tcattatatc ctcgtacttg
    23641 gaaccgttgc aagatatctc tgactacttt gattgcttga tcgatgacag gatcttggat
    23701 ataaaaatct tcgataggga ctagatcatg gctattcttt ttgtaaaaac cggtggctaa
    23761 ctgtccatcg atacggcgaa cagggatctg tgctttatta cggtagccga atggttctgc
    23821 cattccgacc gttggaagta caggaacttc tggcatctta gcaatcttcg ccattacatg
    23881 ctctacttgt ttttgtttga agacaagctg ttgttcatac ttcaaatggg ctaaaggtgc
    23941 gatacctatg cgaagtaatg cattgttttc tacaggctga cgatcaggtg attgtgtaat
    24001 aaatgtttcg acttttccat atccaaactt attgcctgct tttactacta gtatttctac
    24061 ttcttctcca gggagtgcat tttcgataaa taatggataa ccatcgattt ttgctacccc
    24121 cattccttca taactcaagt cgacgatttc gactgtatat cgttcgtttt tctttactat
    24181 ttgctgttct gtcatatttt gctcctttaa gcctattttt caatcctact gaactctact
    24241 ataaatcagc taattacaca actaaaaccg gcactgacct tactacgttc agcaccggct
    24301 tttgattaat tcttttgatt aggtgtttcg tctgctattt ttccgtcacc gtcgatatct
    24361 tcatccgtta aacgttcgac ttcttttacg aattctttgc tgacaacctc taattcttct
    24421 tcatctgtac ctgtgattgc ttcatcagga atctgatcgg tattggcaaa gaactcaatg
    24481 tgttgatgaa gattttggaa atgcatcggt gcttccccgc cgtattcacc atctaagttg
    24541 atcattactg gtcgattgat atcatcttct gtttcaacga acaaactgct tgttttggta
    24601 tagattattc ttggatcatc gacatgtcga ccgccattca gcatgagtgc tgcaatatgc
    24661 aagatctcaa agatatttgc tgtcttcaca atgattaaag aaaacttccc atcatccaat
    24721 ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt tcgtcagccc taggaaaaac
    24781 atcgaagcat ttccgatata ttccccttca tcatacttca agcgcatttt gattggcttt
    24841 actcgaggga gcatctctgc tcctttcgca agataagcaa gataaccaaa aatactcttt
    24901 agttctgatg gaacttcata agtcaattct gtcaaatagc cacctgcagc gatattgatg
    24961 aagtaactgt tatcagaaag gccgatgtcc atttttacgg tttggtgttt ttttatcact
    25021 tctgctgctg ctttaatatt atctctagga atttttaatg ctcgagcata gtcgttcgtt
    25081 gtaccagctg ggatgatggc cattttagga cggtgttcaa gcggagcgat tccattaacc
    25141 acttcgttga ttgtgccatc gccaccagct gccactacta gatcaaaacc tagttctgcg
    25201 actctacatg cttcattctt ggctgaatct ggttctggtg tagttgcata ggcgctggct
    25261 tcaaatcctg cttcttctaa tgcctgtagg atatccgcca aatttttttt caataattct
    25321 tttcccgagg tggggttata aattacgcgt gcttttttca ttttcagcct ctatcgtttc
    25381 gctaattcat ctttaagtag cttattcacg acaccaggat ttgcttgtcc tttagttgct
    25441 ttcatgattt gaccgactag gaagccaacc gcacgatctt ttccattttt gaaatcatcg
    25501 acagattgtt ggttgttatc caaaatttca ttaatgattg gcagtagttt gttcggatca
    25561 gataattgca cccaaccatt tttttcaact acttcttggg cttctccgcc atttgtgatc
    25621 aattcacgga agactttttt ggcgattttt gagctgatcg ttccatcggc gatcaaacga
    25681 atcattcctg ctaagttctc aggtgtcaat tttgtctcag ctaattccag tttctcactg
    25741 ttcaagtaag cagaaacttc acccatcaac cagttagatg cttgcttggc atctgcacca
    25801 ttttccaaaa cagcttcaaa aaaatcagac atttcttttg taagtgtcaa taccattgcg
    25861 tcatactctg gaagtccaaa ctcacggata tagcgttcgc gacgagaagc tggcatctca
    25921 ggcaaactag cttttacttt ttgaatccat tcgtcatcga tcacgaattt cgggatatca
    25981 ggctctggga agtaacgata atcactagac ccttctttga cacgcatcaa aactgtttta
    26041 ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt ctcctcctga aagaagaact
    26101 tttgcttgac gtttttcttc aaaagctaaa ccttttttca cgaaagataa agagttcaag
    26161 tttttcaatt ctgctttcgt accgaactct tcttgtccat aagggcgaag agaaatattg
    26221 gcatcacagc gcatagagcc ttcttccatt ttcacatcgc tgacttctgt aaattggata
    26281 attgatcgca atgcttctag ataagcgtat gcttcttctg gtgaacgcat atcagcttca
    26341 gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat caacgtaaga ataaccgcca
    26401 ataccatgca tgttttttcc agcatcttct tctaaatgga cacgttcgat ccggattttt
    26461 ttctttttac cttctacttc gatttcgatc catccgtcat gaccgattgg ttgatcaaat
    26521 tgggaaattt gatatgcttt tggattatct gggtaaaagt aatttttccg gtcaaaatga
    26581 gtatctttag agatttcaca gtttaatgcc aaagctgctt tcatcccgaa ttccagcgca
    26641 gctttattca ttactggcag cacgcctgga taaccccagt cgatcacgtt tgtattgctg
    26701 tttggttctg caccaaaatg agctggcgct ggagagaaaa tttttgagtt ggtttttaac
    26761 tcgacgtgga cttctagtcc aatgactgtt tcaaaattca ttcgtctttc cctcctaaaa
    26821 tagctggttg ttgtttatgg aattctgttg cttgttcaaa cgcatatgcc gcttgataca
    26881 tcgttttttc atcaaaataa ttaccgatga tctgcaatcc aactggtaat ccttctgata
    26941 aacctgccgg tacagacata cctggaagtc cagccaagtt gacagggatc gttaagatgt
    27001 cactcatgta cattgtgatt ggatcgttaa tgttctcacc gattccgaat gcaactgttg
    27061 gtgaagatgg cccgatgatc aagtcataat ctgcaaagac tttttcaaaa tctcgtttga
    27121 tcaatgtacg gacttgtcct gcttttttga agtaagcgtc ataataaccc gcgcttaagg
    27181 agaatgtacc taacatgata cggcgtttta cttcttcacc gaatccttct gaacgtgagt
    27241 tgacatatac atcatctaat gatttcacat tttcagagcg ataaccgtag cggataccat
    27301 cgaatcgttg caaattggaa cttgcttctg atgaggcaat gatataataa acagccacac
    27361 cgtattttga atgaggcaag ctgacttctt ccacaacagc acctaatgaa cggaatgttt
    27421 ctgctgcttt caatacagct tcttttacgc caggggccac accttcgcct aagtattctt
    27481 taggtaaagc aatcttcatt cctttgatgt cgcctgtcag tccctctgca aaatcaggaa
    27541 ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc gctgatagct gtcaatgcta
    27601 atgcattatc tttcaccgta cgggtcattg gtccgatttg gtcaagagat gaagcaaagg
    27661 caatcaaacc aaaacgtgag acacggccat aagtaggttt catccctacg atgccgttaa
    27721 aagctgctgg ttggcggata cttcccccag tatcactacc taaagaaact ggaatttgtc
    27781 cagctgcaac tgctgctgca gaaccaccag aagagccacc aggtactttt gttgggtccc
    27841 aagcattttt tgttttcttg aaataagaag tttctgtact tccacccata gcaaattcat
    27901 ccatgtttag cttaccaaca gggatcatat ctgcttgata gattttatcc atgaccgttg
    27961 catcataaat tggttcaaaa ttgtaaagga tcttagatgc agcggttgtc aagatatctt
    28021 ttgttacgat attatccttg ataccaattg gaataccagc aaggacgttg ctttcatcaa
    28081 tccctttttc atctaaagct tttgcctgtt ccaatgcttt ttcttcgttc aatgtaatga
    28141 atgcctcgac ctcaggctct gtttctttga ttcgttcaaa tgttgttttt gtcaaatctt
    28201 gtacagtgat ttctttcgat acaagcatat gatgcagttc ttctagagaa cgattgtgta
    28261 attctgtcat tatgcgccag cctccccatt atcgatgatt gcgggtactt tgataaagcc
    28321 gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg ttccatccag cttgcgcccg
    28381 atcttgtcgc atcacgttta ctgtttcaac cacattcgac gtaaatggaa caccttccgt
    28441 atctacttct tccagcattt caaccatatc gatgatttta cctaattggt cggtaaaact
    28501 tgcaagttct tcatctgcga acgcaagttt tgctaatttg gcaacgtgtt tcacttgttc
    28561 ttcactaatc gccatactac cccttctttc taaacgagtt aaaaactttg ctctctattt
    28621 taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt tgtctgaaaa aactcatttt
    28681 tcattcttct actgagcgac ttttagccaa tgcatctgcc atttgatctt ctgtccaaac
    28741 ttcgattcct aattcttgtg ctttggtcag cttgctgcca gcatcttctc ctgctaccac
    28801 gatatccgtt tttttcgata cgctaccagt cactttcccg ccaagatttt cgatcgtctc
    28861 ttttgcttct tcacgagtaa atcgtgttag tttaccagta aggactactg ttttttcttt
    28921 aaaaggagac tcgacttctt gcaattgggc atttcgaagc cctttgtatt cgaaattgac
    28981 gcctgcttgt tttaattcat tcatcaattc atggacttct tcgttactga aataagtgac
    29041 tacactgtcg gcaatgatat caccgatcgt atcaagcgca ataatcgact catagtcact
    29101 tttactcaat gtttctagat caccgaaatg ttctgctaaa atttttgctg ctttcgctcc
    29161 gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc gaattatctt tgctgttgtc
    29221 gattgctgtc aaaatattat ttgctgattt ttcttttatt ttgtccaacg tcaataactc
    29281 ttcttctgtc aatttgtaaa gatcagcaac atctgctact agttttttat catacatctg
    29341 ttctagtacg cgcggcccca acccatcgat attcatcgca tttcttgaaa caaagtggtt
    29401 caaaccttct ttcatttgag cagggcattt aggattgatg caccgtaagg ctacttcttc
    29461 atccaaatgg acaagttcac ttccacaaac tggacaatgt gtaggcaatt ggtattcttc
    29521 gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact tctgggatga tgtctcccgc
    29581 tttataaatc agaactgtat ctttcaagcg gatatccttc aacttaatat aatcaccgtt
    29641 atgcaggctg gcacggctta ctgtcgttcc tgctactcgg actggtgtca tgatagctgt
    29701 tggcgttact acccctgttc gaccgactgt ccattcgatg ttttcgatta gcgtttcaac
    29761 ttcttctggc ggaaacttgt aagctgctgc ccatctcggt gctttgatag taaaaccaag
    29821 ttgatcttgt agcgagaatt cattcacttt gatgacgatt ccatcgattt catagggcaa
    29881 atccacacgt ttgtcatgat attcttcaat gtatgaccac acttcatcaa tcgaatgaca
    29941 tagacgtttt tcccgattcg tgtggaatcc aattttttct agctcttcta gcgcatcata
    30001 ctgggttttt gcttgcatag gaccgaaatc agctactgtg tataaaaaag tgtccagatt
    30061 tcttttcgct gtgattttag aatctaactg acgcaaagaa ccagctgcgg cgttccgagg
    30121 attcgcaaag atatcttttc cctctgcttc tctgtcttgg ttcaattgga caaatgaccg
    30181 ttttggcatg aaacattcgc ctcttacttc gatgttcatt ggttctttta atttgattgg
    30241 gaccgaccgg acagttttta ggttttcagt gatgttttct cctacagttc catccccgcg
    30301 agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa gaaacggaaa gtccatctat
    30361 ttttagttca cagcaatagt caactacacg tcccactgct ttttgtacac gttgatcaaa
    30421 agctatcagc tcttctttgc tgaaaacgtc gttcaaactg taaagcggaa tatcatgagt
    30481 gactttttca aaaccttcta atacttttcc gcctactcgt tgagtgggag aatcagacgt
    30541 gatcaaatca ggatattgtt cctcgattgc tactaattct gcgtattctt tatcataaac
    30601 atagtcttcc actgttggtt tatcttctac gtagtattcg cgagaccatt ggttcagtct
    30661 tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc gtcatttcat gttccatcat
    30721 ttaccctcca ctactaagct ttttcaattg gcgcaaaggc agccaaaaga cgttttactc
    30781 cttgacttgg gaaagccaca tctagttcta gatccttcga cgtcccgctt actcggacga
    30841 ctgttcctag accccatttt ttgtgtttga ctttatctcc aggtttccag ctgttatttt
    30901 cccctccagt agcactttta gagggaacaa ccgctgtctc tggctgttta tagctatatg
    30961 ctgttttttt agcgaataca ggcgtttttt taggcgtagg acgcatgcct tcgatttcca
    31021 gtaactcctg atcgatttct tctacaaaac gagaaggtcg gttatattgc gtccgtccat
    31081 ataatgttct ggagaaagca tttgttaaat acaacgcttc ttctgctcgc gtgatcccca
    31141 catatgcgag acggcgttct tcctccagtt cgctttcttc catcaatgct cgagataaag
    31201 gaaaaacccc ctcttctaga ccaatcagga atacaactgg aaattctaat cctttggctg
    31261 catgcaaagt catcaatgtc acttgggaag catcttcttc taaattgtca atgtcggaaa
    31321 caagtgccag atcatttaag aagaccgtta gtttttcttc tggtgcatca gcgtcttctt
    31381 cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac cgttaaaaat tcctccaagt
    31441 tttcgatgcg tgcttgtgct tctaatgtat tttggatttt taagtcttct agatatccgc
    31501 tacgatccaa aacctctttc gtcaattctg tcactgtcaa gtatgggatc atctgagtga
    31561 cttcttggat catctctcca aatgcaccta attgctgtcc tgcttttcca ccgatatttg
    31621 ccagatccac attttgcgct gcttcaagca gtggccattc atgaagagaa gcaaaactgc
    31681 gtagtttttc gatagatcct ggtccgatgc ctcgtttggg cgaattaacg atacgctcaa
    31741 aactgattga atcttgagga tttaccagca cattcaaata cgctaaaata tctttgattt
    31801 ctttacgatc gtagaacttg tgccctccaa ccattttata aggaatattt gctttcaaca
    31861 gagtttcttc cattacacga gattgtgcgt tcgtccggta caagatggca aagtcgccgt
    31921 aattccgatg attgcttcga atctcttctt gcatccggtc tacgataaaa cgggtttcgt
    31981 ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc ttctttgttt tctgtccaca
    32041 agttttttgg ttttcgattg ctattgtttt caatgacttg gtttgctgcg ctaaggatgt
    32101 ttttcgtgga gcgatagttt tgttctagca aaattactgc agcatctgga taatcttttt
    32161 caaaatccaa gatgttctgc atatctgcac cgcgccagcc gtaaatactt tgatctgcat
    32221 ctcccacgac gcacaggttc ctaaatctac ctgccaataa attgactagc gtatactgtg
    32281 cgtgatttgt atcttggtac tcatctacat gaatataatg gaatttgttt tgataatacg
    32341 tgagtgaatc aggatgttct tcaaataatc gaatcgtatt catgatcagg tcgtcaaagt
    32401 ccatacattg attatattga agctcttttt ggtaagcagc ataacatttt gccgcaatct
    32461 cttcgtaata actgccttgc atttttgcaa aatcctgagg ggtctgcagg ctgttttttg
    32521 cttgagagat tgttcctaat atacttcgag gatcatattt tttcggatca atgttcagtt
    32581 cttttagtat ccgtttcatc aaggtcagct gttcagaaga atcgatgatt gtaaagttcc
    32641 ggttataccc gataaaatca acatctcttc ttaaaatacg cacacacatc gagtgaaaag
    32701 tcgataccca gacatcttcg cctccagatg cgagaatcgc attgacacgt tctttcattt
    32761 cttttgcagc cttattggta aaagtaatcg ctaggatgtt ccaaggattg acttcttttt
    32821 cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt ctttccactt cctgcacctg
    32881 ccattagcag taatggtccg tcagtgtgaa gaacagcttc tttctgacgg gggttcatcc
    32941 cgtttaataa ttcagctttg gatgacatta acaacatcct ttcttttatc aatcttgtta
    33001 attatagcat tttcttggct ttcattaaac tagctagttc aaaatcattt gtttcttttt
    33061 atggattttt ccttgtcaat tcgttgattt cgcgctaaat taaaagaaag cgaggcgttt
    33121 gaatggaaaa aaaaggaaaa atcaagaaat tagataagac tcagatatat tttattctaa
    33181 aaggaatatt tgttggactg ctgacaggaa tcgtcgttag cctgtttcga ttatctatcg
    33241 aaaagctaag cgatttagtt cgaagtatct atgaaatgag tcgggaacag ccgatttact
    33301 taattggcct agctatttgc tgcatccttg cagcattttt cgtgggttat ctggtcaaaa
    33361 atgaaccgga tatcaaggga agcgggattc cacaagtaga gggacagctt cgcggggaat
    33421 tatcaatgaa ctggttctct gttttatgga aaaagttcat tggcggtgta ctttcagttg
    33481 gtgcgggcct ttttctaggt agagaaggac cttctataca gcttggcgca tccattggac
    33541 aaggagctgg acaattgttt cggagtccat cttcagaaga aaagatttta atttctagcg
    33601 gtgcaagtag cggacttgca gcagcattca atgctcctat cgcaggatta ctctttgttt
    33661 tagaagaaat ccatcactcc ttttctccac ttgtttggct gacatcattt gcttcagcaa
    33721 tcactgctaa cttcgtctct ctttactttt tcggcttgag acctgtttta tatctaggtg
    33781 accttccttc tttgccgttg aaatattatg gttccttagt gatcttagga gtacttctag
    33841 gcatcttagg ttttatttat cagaatgtct tgctggcttt gccaaaattt tacaaccgat
    33901 tgcctttacc agcttatttt cacgggttcg ttcctttctt gctgattttg ccaattggtt
    33961 atttatggcc tagtactcta ggcggaggca atcaaatcgt tttagctttt ggacatgctt
    34021 ccttgccaat cattatgatt atcggtttgt ttgttcttcg attcgttttt tccatgattt
    34081 cttacggtgg gggtcttcca ggggggattt tccttccgat attgacattg ggggctttaa
    34141 tcggtagctt ttatggaaac attatggttg atttcgggat ggatccaatc tacatcaagg
    34201 attttgtcgt tattgccatg gctggttatt tcacagcgat tggaaaagca ccgctgacag
    34261 caattatttt agtgacagaa atggttggct ctcttaatca tctgatgcct ttaggattgg
    34321 ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg taatccaatt tacgaatcat
    34381 tattggaacg tctacttgga aatactcaga ccgcctcact aaaaggtgca aggacaactt
    34441 ttgattttcc agtaacggca gagagttctt tagacggatt aatggtacga gacttcaatt
    34501 ggcctaaaaa tatgctgcta atctctatac gcagaggaaa tcaggaaatc ttgactcacg
    34561 gtgatacgat catgaatgta ggagatattt tggttatctt gactgatgaa gcaaacttgc
    34621 caagaatcaa gcaggaaatc gaggctaaat cttctatcac aaatttgaag caatcatttt
    34681 aacgaacatg gaaaaactat tttacaacaa ctattcatct acactacttg attctatatg
    34741 attctataaa aaaagaagag gctgggaaaa
//
LOCUS       DAFCNS010000024        34661 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFCNS010000024 DAFCNS010000000
VERSION     DAFCNS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.024"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(313..2205)
                     /locus_tag="LNE23_001099"
     CDS             complement(313..2205)
                     /locus_tag="LNE23_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6930970.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="LNE23_001100"
     CDS             complement(2202..3929)
                     /locus_tag="LNE23_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6930971.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="LNE23_001101"
     CDS             complement(3945..4595)
                     /locus_tag="LNE23_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK6930972.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="LNE23_001102"
     CDS             4799..5095
                     /locus_tag="LNE23_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK6930973.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="LNE23_001103"
     CDS             5287..5907
                     /locus_tag="LNE23_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBK6930974.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="LNE23_001104"
     CDS             complement(5999..7165)
                     /locus_tag="LNE23_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6930975.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="LNE23_001105"
     CDS             complement(7177..7530)
                     /locus_tag="LNE23_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK6930976.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="LNE23_001106"
     CDS             7658..8680
                     /locus_tag="LNE23_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBK6930977.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8699..8860
                     /locus_tag="LNE23_001107"
     CDS             8699..8860
                     /locus_tag="LNE23_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930978.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9119..9553)
                     /locus_tag="LNE23_001108"
     CDS             complement(9119..9553)
                     /locus_tag="LNE23_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930979.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="LNE23_001109"
     CDS             complement(9663..11522)
                     /locus_tag="LNE23_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK6930980.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12799)
                     /locus_tag="LNE23_001110"
                     /pseudo
     CDS             complement(11640..12799)
                     /locus_tag="LNE23_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13106..14530)
                     /gene="araA"
                     /locus_tag="LNE23_001111"
     CDS             complement(13106..14530)
                     /gene="araA"
                     /locus_tag="LNE23_001111"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBK6930981.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14546..15244)
                     /locus_tag="LNE23_001112"
     CDS             complement(14546..15244)
                     /locus_tag="LNE23_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK6930982.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15201..16862)
                     /locus_tag="LNE23_001113"
     CDS             complement(15201..16862)
                     /locus_tag="LNE23_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBK6930983.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17080..18480
                     /locus_tag="LNE23_001114"
     CDS             17080..18480
                     /locus_tag="LNE23_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBK6930984.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPTIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18732..19811)
                     /locus_tag="LNE23_001115"
     CDS             complement(18732..19811)
                     /locus_tag="LNE23_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6930985.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19897..20856)
                     /locus_tag="LNE23_001116"
     CDS             complement(19897..20856)
                     /locus_tag="LNE23_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK6930986.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20868..22379)
                     /locus_tag="LNE23_001117"
     CDS             complement(20868..22379)
                     /locus_tag="LNE23_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK6930987.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22401..22964)
                     /locus_tag="LNE23_001118"
     CDS             complement(22401..22964)
                     /locus_tag="LNE23_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBK6930988.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22970..23833)
                     /locus_tag="LNE23_001119"
     CDS             complement(22970..23833)
                     /locus_tag="LNE23_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6930989.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23847..24743)
                     /locus_tag="LNE23_001120"
     CDS             complement(23847..24743)
                     /locus_tag="LNE23_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6930990.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24918..26207)
                     /locus_tag="LNE23_001121"
     CDS             complement(24918..26207)
                     /locus_tag="LNE23_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6930991.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26179..27696)
                     /locus_tag="LNE23_001122"
     CDS             complement(26179..27696)
                     /locus_tag="LNE23_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK6930992.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27894..28784
                     /locus_tag="LNE23_001123"
     CDS             27894..28784
                     /locus_tag="LNE23_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBK6930993.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="LNE23_001124"
     CDS             complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="LNE23_001124"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBK6930994.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29693..31165)
                     /locus_tag="LNE23_001125"
     CDS             complement(29693..31165)
                     /locus_tag="LNE23_001125"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBK6930995.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31802..32164)
                     /locus_tag="LNE23_001126"
     CDS             complement(31802..32164)
                     /locus_tag="LNE23_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBK6930996.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32387..33139
                     /locus_tag="LNE23_001127"
     CDS             32387..33139
                     /locus_tag="LNE23_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6930997.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33279..34202)
                     /locus_tag="LNE23_001128"
     CDS             complement(33279..34202)
                     /locus_tag="LNE23_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6930998.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34217..34489)
                     /locus_tag="LNE23_001129"
     CDS             complement(34217..34489)
                     /locus_tag="LNE23_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6930999.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10230 a         7213 c         5980 g        11238 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg
    12061 gaaaatcagt caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt
    12121 tatcaacaat ttcacttctt cctacattga taagtacagc atcttttttc attttttcta
    12181 tattcgcctg atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt
    12241 aatccgaggt agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat
    12301 cgcggataga ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg
    12361 tgcctatcgc tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat
    12421 aagcacttct tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca
    12481 tacgacttgc ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt
    12541 ttactgcatt agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt
    12601 tttcaacgtt gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg
    12661 taagccattc atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat
    12721 ctattatttt gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa
    12781 tcaaatgaac gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca
    12841 attagacaac tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta
    12901 ggctgagtga taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc
    12961 aaaatattga gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa
    13021 gactaaattt caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac
    13081 taagtggctt tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt
    13141 ttgcccacgt ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc
    13201 cacctgcctg aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca
    13261 cacgtgcaac aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga
    13321 tcagcagttt ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt
    13381 tcccatcaaa tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa
    13441 cgattcttgg tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga
    13501 ttgcttcttg gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc
    13561 ctcgagccat gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag
    13621 caaaaccgta tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc
    13681 catacaggtc ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc
    13741 gaataccgat ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat
    13801 aatcaccata gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt
    13861 cgtctgatac gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc
    13921 aaccaaattg aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt
    13981 tgtcaccaaa acgagccact tttattccaa aactttcatt ataggctact gcaacatcca
    14041 tccaatcagc aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat
    14101 tttgtttatt taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat
    14161 ttaaattcat gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag
    14221 ctaaatgaag caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg
    14281 agaaagtatg catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca
    14341 tgatttttgt gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca
    14401 attggccatt ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca
    14461 gtgcttcttc cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa
    14521 tattcaacat aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt
    14581 gttttcgtaa ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga
    14641 gctgtctggt aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg
    14701 cactttgcgc atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa
    14761 ttgctaaggg atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat
    14821 attcgtaccc gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat
    14881 aaaaagtatc tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga
    14941 tcgtcgccca tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata
    15001 aaacagcatg agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt
    15061 ccaagtccac aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt
    15121 tgataacgaa atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga
    15181 cgagtccgtg tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca
    15241 gcattttcta tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc
    15301 ccctcacggt aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg
    15361 atggtgattc cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc
    15421 ccatgtcgat ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg
    15481 gctgtgtcca tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact
    15541 tctggtgttt taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat
    15601 ttcaatatat ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg
    15661 aaattcgcta aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt
    15721 actcctgtaa tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta
    15781 tcgccttcta gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata
    15841 cccaaagaat tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag
    15901 ttattcgtat gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc
    15961 ggatggacat tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc
    16021 gaaatgttcc ctgtcccttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc
    16081 ccttctggcg gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa
    16141 gcccctgatt cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg
    16201 tgatccaagt caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt
    16261 tcttgatggt aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg
    16321 cctagtactt tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa
    16381 ttaatttctt tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc
    16441 catctctgcg gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg
    16501 attgtatttc gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga
    16561 taaccgtgca tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag
    16621 atttcgaata cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct
    16681 tctaatgaat aggtccagat accattttct agtttgtttt cccactcata actaccagcg
    16741 gcaataggag taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca
    16801 atccctaaag atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc
    16861 ataatgattt aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct
    16921 tacaatgatt attgtatact tttgtacgta catgtcaagc agttatttac aagaatacaa
    16981 taaaaaacac ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga
    17041 tcaaccgcct tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt
    17101 tcctcatcgt ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt
    17161 ggtgttatga ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct
    17221 tctttgatag gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta
    17281 tctggtcttc tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt
    17341 tttatggctg gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt
    17401 gcagcaagga ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat
    17461 atgtctgaaa tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg
    17521 attgcttctg gcatgctcct ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa
    17581 acgatggctt ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc
    17641 ggtgttttag ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag
    17701 gcaaaaagag tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa
    17761 atccagctga atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta
    17821 gaaaaatatc gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa
    17881 ggagctaatg ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca
    17941 gcgagcgatg cattgatgtg gccgaccatc caaggaatca ttttagttgc tggttctttg
    18001 ctctttttgg ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc
    18061 gtgatgggac tttctttcat cctaccagct gttttaggaa ctgtactgga tgctcataca
    18121 aattcgttgt tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg
    18181 gctccactga catgggtaat cgtaggcgaa atattcccac tagctgtccg cgggcgagca
    18241 tctggcctag cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct
    18301 gtgatgactg cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt
    18361 ttaggggttt tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa
    18421 atcgaacaaa gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa
    18481 agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt
    18541 cacaaggaac aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc
    18601 agttggtagg agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg
    18661 atgacaaaag ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa
    18721 aaacccgtct cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca
    18781 tagagaatat ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca
    18841 tcttgtccca acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg
    18901 cttaaagaag aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt
    18961 tctgtcaact tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct
    19021 aaagcttgtt cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc
    19081 gtggtgaaag taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg
    19141 ataaaacctt tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga
    19201 cgctgatgtc ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct
    19261 aaacaaatat aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg
    19321 ccttgttctc tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta
    19381 ggctcaacga tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc
    19441 aattcgtgat cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca
    19501 ttttcgattc ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca
    19561 ccgatcgttt ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat
    19621 gtgcccgacc ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc
    19681 cgaatcgtat gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt
    19741 gtatttactt cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga
    19801 tattttgcca tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc
    19861 aaggtttatt caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc
    19921 accaaattct ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt
    19981 aggattgtct aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaaat
    20041 atcaaagcac ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt
    20101 tttttcagaa gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag
    20161 attcttgtgc tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc
    20221 agaatacgta atgaatattt tcccattgcg agtaaggact gctggacctt catttactga
    20281 aaaaccccgt ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa
    20341 cgtccaagga ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc
    20401 ttgttgggcc cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact
    20461 gaaagtttcc cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc
    20521 tatatgctga ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt
    20581 atgattgctt gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc
    20641 ccagatcaat tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc
    20701 atgttctaat tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac
    20761 cgatcctgta aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg
    20821 ccgaacgata ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt
    20881 attctaatta cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct
    20941 attgaaaaat agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc
    21001 aacagatcgc tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt
    21061 tcaatcgtta tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg
    21121 ccgttatcta cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa
    21181 acagggcaaa tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc
    21241 atcacataat aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt
    21301 attgtgttta ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata
    21361 gccagcccta atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg
    21421 ggactaccga cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca
    21481 tcaaacgcta gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta
    21541 gcaacgattt cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta
    21601 tcatcacgct catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag
    21661 ttatgcaaag ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat
    21721 tcagggtacg atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca
    21781 tccactaact tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc
    21841 ttagccccca cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca
    21901 aaaggctttt tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa
    21961 ttacaatatt ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg
    22021 aatatcggtt tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa
    22081 tttggttcta tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct
    22141 acagtatcca cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct
    22201 aattctaatt cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt
    22261 tcatggcccg gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag
    22321 ccaaacagcc gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact
    22381 tcttcgtcca acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt
    22441 ttccatacgc caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca
    22501 aatcctaata acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat
    22561 gcactttgtt caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc
    22621 caggataaaa gaaacatgga ataaataagt aaaactacat atccactaat tgaaagtacg
    22681 attgtctgtt cttctaataa ttgattaaca agaaaataaa taatgatcgt taacagacta
    22741 aggaacaagt tcttaccaaa atgttgcttt attccttgaa agaaaaaata aatgatattt
    22801 cctgatccct cacctttcca ttgcgccatt acataaaaaa cagcacatgt agcaggcacg
    22861 atagtaataa caggcaaaca cacgattacc cagagaatac ctaataagat gtaatcagaa
    22921 accacaatca actgtccata aagtttagtc tcaactattt tcatcttcct cactctttca
    22981 ttccagacat agctgttcct tgaacaaaat atttctgtgc aaaaatataa accactagta
    23041 atggcagcat tgaaataacc gttccagcca ttacaggtcc ataaaaagaa atgttctgcc
    23101 ctgtaaataa agctaaccct gctggtaaag ttctcatttt tgaactactt gtcaatatca
    23161 atggataaag caagtcattc caactattca tcagcgtgaa aatccctaat gataaaagag
    23221 taggtttgct caatggcaac atgattttca aaaaaatatt aaactcactt aatccatcaa
    23281 tgcgtgctgc ctcttccaaa gaatctggta aagtaataaa aaaggaacgc atcatgaaga
    23341 taccaaatgc agttgtcata cgaggaatga tcaaacctgc ataagtatca agcatgccaa
    23401 gcaaattgac ttcaataaat agaggaatca tgaagacttg gaatgggatc atcattgtca
    23461 gcaaaacgaa atagaacaaa actgattttc ctttgaaacg catacgagca aatgcatacc
    23521 ccgcaagaga atcaaagaat acagaagtga tcactgtccc tccagcaaaa atcactgtat
    23581 ttttgatgta atctaataaa gggatcgttg tccaaacctt aatatagtta tctagggtat
    23641 agctatctgc aaatattcta ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag
    23701 aaataatcag ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc atactaatcg
    23761 ttcgaatgat ttttttacca ctaactgctt ttcgactatt cacagtaact gtaatcgagc
    23821 tttctggtgc catacaggtt tccctcctat ttcatttgtt tttctttccg tgacatgtac
    23881 acattagtgg agatagctat agttgcaaca atgataaaca aaaccgaaga cagtgctgat
    23941 gcatagccca attcataagg tgctgtaaat cctcgactgt aaatatattg aacagctgtt
    24001 tctgtcttaa attgtgggcc accagcggtt gcaacatata cttggtcaaa cacttgcatc
    24061 gaaccaatca gattcgttaa aatacaaaaa cctaaggagg gaacgatctg aggtagcgta
    24121 atgttaaaaa actgctgtcg atttgttgca ccatccattt gagcagattc atacaaagag
    24181 ggagagattc cctgaatagc agtcaagaga atcgtcatag taacaccaaa attcttccaa
    24241 acagtcatga tggctaccgt cggcatagca agggttggat cgcgaaaaaa ttgagggatc
    24301 ggcaagccga cttgtgaaaa caagtaagga atcgccccta cattcggatc aagcaacata
    24361 gaaaaaataa tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga
    24421 caaaaacgat tgaatctggt atttttagat aatgctactg ctgctaataa accaacgata
    24481 atttgtgttg gcgtttcgag tacggtgaaa tataaagaat gaatcaacga attaactgct
    24541 cgttcatcac taaatatacg tgtgaaattt tcaaaactta caaaatttct tgatgtaaag
    24601 aaaatattca aatctgtaaa actaatagca aatgttccta aaagcggtat gacagtaaat
    24661 aataataaaa ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc aggacgattc
    24721 caatatttgg tgaatccgtt catatagtcc acctcatctt tcattttgat aaaatataca
    24781 atcttggttt aaacgaatct gaataagata tccgaacaat cgctgtgatt tttgcaataa
    24841 cacctatctg caaatactag gtcgatcatt cagatatctg tatcttgcca acagtctatc
    24901 ctgtataaag ttctgtacta tttttgcagt aattgattga tggcatcatc tgcatcgttc
    24961 atgagtgtct caggatcttc tcctgctaat aatttttcaa tcagtggatt gatgatatcg
    25021 ttattgatcg cagagatttg ggtaaaacct ggtaaatatg gctctgcata gtccatttgt
    25081 ttcgataatt cagatacaat aggatcattt tttacttctt catcttttgc tactgaatcc
    25141 aagtaggcag gaaaaccatt ttcgatactc cattttttac caatttcagt tgtattccaa
    25201 tatttcaaaa actcatagac agcttctttt ttattattat ctgtacttgc cggaatggca
    25261 tatcctacgc catctaaaat agctgttttt ttgtctgaat cactcccttg tggtaaggta
    25321 gtcacattat aatcaatctc gtttgctttc aatccgttat ttaaccatgg cccattgatt
    25381 tcaatggcta gttgaccagc attcattaaa ttgtccattt cggcgccaga aatcgattct
    25441 ggaccagtct tgtctacatg gatcattttt tggatcgctt ttaacgtttt aaggttctca
    25501 ggagacgcaa attcagactt agtataatca tcgttcacta atttaccgcc atttgctaac
    25561 atccagttat ataacggtgc agttccatcc ttattgaaga caaaacctga tacattttta
    25621 ctactatcag ttaattttgg tgccatttct gtcaattctt cccaagtagt aggtggttgt
    25681 tcagtatcta atccagcttg tttaaacaaa tcattattcc aatacatata catgccttgt
    25741 acctgcatgg ggacaaaata ctgtttacct tctacttcac ctaattcaat agccgtttct
    25801 ttaaaatcag atttatctac tccatcatat tcccagaaat cgtctaatgg ttgtaccgct
    25861 ccgttttcta catactgtgc aaaatctgga taatttaatg caataaagtc aggtgctttt
    25921 ttagaagaaa tcgctgtagg aagtttttca ttcaaattgg cccaagtcat gatatccatt
    25981 tcaattttga tgtttttatc atttgtttta ttaaattcat taacgatttc ttgcaatatt
    26041 tcaccatcag atgcagtaaa cccattccaa aaagtcaaag tcacttcttt atcagaatca
    26101 gattctgctg aacttccgcc accgcaagct cctaaaatac ctgttgcagc taccaacaaa
    26161 gagccaatta accatttttt acctttcatt ttttgttcct ccttcatctg ataaacgaca
    26221 tctgaccaca ttccaagata gcggtttcaa taaacaattg attttcccat cttcagcttt
    26281 tacatttgtt gaatgacttg gttttacttg ttcactttta ggaagattag ttgcttttgt
    26341 atcaaatcca ctcatttcag aaaaatcaat cactccctct aatgataatt cagacaagtg
    26401 aacagttaga ttcatttctt cttctccacg gttaactgca aatatgacaa gttctttgtc
    26461 atttattacg gctatacttt ctaaatacgg cacttcttca aaatcttccg tcgcatagtt
    26521 tgctgaacga atttgtgggc ttaacacctc tccttgtcca tacacagcag cttgcataaa
    26581 tggataatag atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat
    26641 aacatttacc aattgagcaa gacaagcaat ctttactcta tctgcatgtt tcaataaagt
    26701 gattaataga cagcctaata gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg
    26761 aggagcaatc tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat accaaatatt
    26821 ccattcatca aaggacaggt tgatctgctt cttactatgt ttcttcgcct tgactgtatc
    26881 gcacatcgct gtcacttcag aaatgaaccg atccatgtct aaggaacgtg caagataatg
    26941 agaaagatca tttttttggt tgccataata ttgatgcaga gacaaataat ctatttgatc
    27001 gtacgcttga tcaagaactg ttaactccca atcaccaaat gtaggcattt gccgattcga
    27061 acttccgcat aaaaccacct caattgaatc atctacgagt ttcatcgcct tagccgtttc
    27121 ctctgctaac cgtccatatt cttccgctgt tttatgtcca atctgccaag gaccgtccat
    27181 ttcattccct aaacaccatg tcttaattcc aaaagggctc tcttgaccat tcgaacgcct
    27241 taaatcactc caataagttc ctttaggaaa attacaatat tcgactaaat tacgagctgc
    27301 atctactcca cgagtaccca gattgacagc catattgatt tcggccccca catgctgaca
    27361 ccagttataa aattcatgaa tcccaacttc attcgtttca attgtccgcc aagctaaatc
    27421 caaccttcta ggtctatttg ctatgggccc aatactgtct tcccaacgaa atcctgacac
    27481 aaaatttcca ccaggatatc taatcatggg aatgttcaat tcttttacca agtgaatgac
    27541 gtcttggcga aatccacttt tattagctga tggatgatct ggctgataaa tcccttcata
    27601 tactgctcgt cccatatgtt caataaatga actgtacaac cgcggatcaa ctttgcctac
    27661 acgcttgaca ctactaatca acattgtggc ttccataact gacctccttt aatttataca
    27721 tgaataatac acctcgttga aagcgttgtc aataatataa atgttaaact tacttatttt
    27781 ttatcgattt taattaacca aaatattaat aatgattaaa acaaataatt taatataact
    27841 tttattaaca aatatgttaa tatataagaa aaacctttta agaggtgata tatatgcaac
    27901 tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa acaaggatca
    27961 agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg
    28021 gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca aacatcatca
    28081 aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa atcgatatca
    28141 gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata cctattggtc
    28201 actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat gattttatcg
    28261 ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc cgcattctct
    28321 ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca gaggatacat
    28381 tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca aatgacaatt
    28441 ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg acgagccctg
    28501 gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat aatcttaatc
    28561 aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg aatggagaac
    28621 ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg catttgcgaa
    28681 tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt aaaggattcg
    28741 gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt tcttttctat
    28801 tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct ttattgttat
    28861 ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac
    28921 catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag ataatcatca
    28981 atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt cccatcttca
    29041 agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc ttgaagcaat
    29101 tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc accatcacca
    29161 aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa tacggctcct
    29221 gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt gatatttcct
    29281 ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg tgcatcaaca
    29341 gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag agcagctttt
    29401 gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa ttgatagttt
    29461 tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg gcctgctaaa
    29521 gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg atattttttc
    29581 aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc aattgttggt
    29641 ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc tcctatgctt
    29701 ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt ttatggttcc
    29761 agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt ttatactctt
    29821 cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt gcaggtaatt
    29881 cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct gtaaagtctc
    29941 gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct tcttcttctc
    30001 gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca aaatttcgag
    30061 tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg cttgataact
    30121 tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg aatactgcac
    30181 caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat acatcaattt
    30241 tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc gaagcataga
    30301 tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta gatatatagg
    30361 ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct aattctttcg
    30421 ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg actaagaaca
    30481 cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt ccataagatt
    30541 ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc ccagcacgta
    30601 cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag actgctgcat
    30661 ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg atgacagatt
    30721 tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc aacagtgctg
    30781 tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt tcatttgcaa
    30841 aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat
    30901 actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata tcggaatcat
    30961 tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa accgcgtatc
    31021 cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac
    31081 ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc agcgtcaaac
    31141 tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt ttcatttcct
    31201 acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc tgaaaaagca
    31261 catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact atcctttctt
    31321 attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc cgagacacga
    31381 agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc ctactacaat
    31441 cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta caacataatc
    31501 aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt atccccaaag
    31561 aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat gacaagatat
    31621 accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta
    31681 agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca gtctcgcctt
    31741 gttttttatt gtatacatta gatatggagc aggttaccat aaagatgatt attagtcgtt
    31801 attaaataga atccttcgta tttttttgtt ctatcactac aatatcttta tttggacgtt
    31861 tgaattgtat tccggcacgt tctagctcct gtttcactcc tacatggact ttgacatctt
    31921 ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt gtgatttttt
    31981 tcttcgtatt gatcatcacg acttgctttt tatcatatgt gtctaccaat agcagttgtg
    32041 ttcgatctaa cgaaaataat ttgtaataat taatagccgt attcccaaaa aagaattcca
    32101 ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt tcgattagct
    32161 tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat
    32221 ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt
    32281 ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt tcttgaccat
    32341 ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat
    32401 aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat
    32461 tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga
    32521 agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat
    32581 ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca
    32641 aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact
    32701 tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca
    32761 acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa
    32821 aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga
    32881 aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga
    32941 agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct cttacagctg
    33001 gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg
    33061 gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat
    33121 tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc
    33181 taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc
    33241 attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa
    33301 ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa
    33361 gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg
    33421 cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc
    33481 atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt
    33541 tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa
    33601 agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc
    33661 aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt
    33721 gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa
    33781 actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta
    33841 atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct
    33901 ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg
    33961 atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg
    34021 cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata atcgacttct
    34081 ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt
    34141 acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc
    34201 actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac
    34261 cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc
    34321 aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc
    34381 tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat
    34441 aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc
    34501 cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc
    34561 aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa attgaataca
    34621 ttataatgat actgtaaata tatctttatt attagtgaat g
//
LOCUS       DAFCNS010000025        34636 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFCNS010000025 DAFCNS010000000
VERSION     DAFCNS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.025"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            591..1280
                     /locus_tag="LNE23_001130"
     CDS             591..1280
                     /locus_tag="LNE23_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6931000.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="LNE23_001131"
     CDS             1267..2475
                     /locus_tag="LNE23_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6931001.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="LNE23_001132"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="LNE23_001132"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBK6931002.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LNE23_001133"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LNE23_001133"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBK6931003.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="LNE23_001134"
     CDS             complement(6578..7273)
                     /locus_tag="LNE23_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBK6931004.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LNE23_001135"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LNE23_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBK6931005.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="LNE23_001136"
     CDS             complement(8472..8663)
                     /locus_tag="LNE23_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBK6931006.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="LNE23_001137"
     CDS             complement(8684..9574)
                     /locus_tag="LNE23_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBK6931007.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="LNE23_001138"
     CDS             complement(9561..10328)
                     /locus_tag="LNE23_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6931008.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LNE23_001139"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LNE23_001139"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBK6931009.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LNE23_001140"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LNE23_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBK6931010.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LNE23_001141"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LNE23_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBK6931011.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="LNE23_001142"
     CDS             14899..15282
                     /locus_tag="LNE23_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931012.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="LNE23_001143"
     CDS             complement(15450..16208)
                     /locus_tag="LNE23_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBK6931013.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="LNE23_001144"
     CDS             complement(16226..17044)
                     /locus_tag="LNE23_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK6931014.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LNE23_001145"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LNE23_001145"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBK6931015.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LNE23_001146"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LNE23_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBK6931016.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="LNE23_001147"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="LNE23_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBK6931017.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="LNE23_001148"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="LNE23_001148"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBK6931018.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="LNE23_001149"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="LNE23_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBK6931019.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="LNE23_001150"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="LNE23_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBK6931020.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="LNE23_001151"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="LNE23_001151"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBK6931021.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="LNE23_001152"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="LNE23_001152"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBK6931022.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="LNE23_001153"
     CDS             27086..27385
                     /locus_tag="LNE23_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBK6931023.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="LNE23_001154"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="LNE23_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK6931024.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="LNE23_001155"
     CDS             27973..28209
                     /locus_tag="LNE23_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBK6931025.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="LNE23_001156"
     CDS             28352..30325
                     /locus_tag="LNE23_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBK6931026.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="LNE23_001157"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="LNE23_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBK6931027.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="LNE23_001158"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="LNE23_001158"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBK6931028.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="LNE23_001159"
     CDS             33204..34496
                     /locus_tag="LNE23_001159"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBK6931029.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DAFCNS010000026        34552 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFCNS010000026 DAFCNS010000000
VERSION     DAFCNS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.026"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(71..2113)
                     /locus_tag="LNE23_001160"
     CDS             complement(71..2113)
                     /locus_tag="LNE23_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931030.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            complement(2103..2897)
                     /locus_tag="LNE23_001161"
     CDS             complement(2103..2897)
                     /locus_tag="LNE23_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931031.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            3196..5385
                     /gene="nrdD"
                     /locus_tag="LNE23_001162"
     CDS             3196..5385
                     /gene="nrdD"
                     /locus_tag="LNE23_001162"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBK6931032.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            5700..6302
                     /gene="nrdG"
                     /locus_tag="LNE23_001163"
     CDS             5700..6302
                     /gene="nrdG"
                     /locus_tag="LNE23_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBK6931033.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(6356..7477)
                     /gene="dinB"
                     /locus_tag="LNE23_001164"
     CDS             complement(6356..7477)
                     /gene="dinB"
                     /locus_tag="LNE23_001164"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBK6931034.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(7586..8458)
                     /gene="rsmI"
                     /locus_tag="LNE23_001165"
     CDS             complement(7586..8458)
                     /gene="rsmI"
                     /locus_tag="LNE23_001165"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBK6931035.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            complement(8527..8874)
                     /locus_tag="LNE23_001166"
     CDS             complement(8527..8874)
                     /locus_tag="LNE23_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBK6931036.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            complement(8867..9690)
                     /locus_tag="LNE23_001167"
                     /pseudo
     CDS             complement(8867..9690)
                     /locus_tag="LNE23_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
     gene            complement(9726..10664)
                     /gene="holB"
                     /locus_tag="LNE23_001168"
     CDS             complement(9726..10664)
                     /gene="holB"
                     /locus_tag="LNE23_001168"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBK6931037.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            complement(10678..11007)
                     /locus_tag="LNE23_001169"
     CDS             complement(10678..11007)
                     /locus_tag="LNE23_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBK6931038.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            complement(11022..11666)
                     /locus_tag="LNE23_001170"
     CDS             complement(11022..11666)
                     /locus_tag="LNE23_001170"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBK6931039.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            complement(11730..12326)
                     /gene="recR"
                     /locus_tag="LNE23_001171"
     CDS             complement(11730..12326)
                     /gene="recR"
                     /locus_tag="LNE23_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBK6931040.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            complement(12351..12665)
                     /locus_tag="LNE23_001172"
     CDS             complement(12351..12665)
                     /locus_tag="LNE23_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBK6931041.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            complement(12685..14430)
                     /gene="dnaX"
                     /locus_tag="LNE23_001173"
     CDS             complement(12685..14430)
                     /gene="dnaX"
                     /locus_tag="LNE23_001173"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBK6931042.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            14645..15892
                     /locus_tag="LNE23_001174"
     CDS             14645..15892
                     /locus_tag="LNE23_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK6931043.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15917..17101
                     /locus_tag="LNE23_001175"
     CDS             15917..17101
                     /locus_tag="LNE23_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK6931044.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            17098..17895
                     /locus_tag="LNE23_001176"
     CDS             17098..17895
                     /locus_tag="LNE23_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBK6931045.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(18337..19032)
                     /locus_tag="LNE23_001177"
     CDS             complement(18337..19032)
                     /locus_tag="LNE23_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK6931046.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            19186..19464
                     /locus_tag="LNE23_001178"
     CDS             19186..19464
                     /locus_tag="LNE23_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931047.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            19483..20691
                     /locus_tag="LNE23_001179"
     CDS             19483..20691
                     /locus_tag="LNE23_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6931048.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(20839..21204)
                     /locus_tag="LNE23_001180"
     CDS             complement(20839..21204)
                     /locus_tag="LNE23_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBK6931049.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(21351..22124)
                     /locus_tag="LNE23_001181"
     CDS             complement(21351..22124)
                     /locus_tag="LNE23_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6931050.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(22298..23158)
                     /locus_tag="LNE23_001182"
     CDS             complement(22298..23158)
                     /locus_tag="LNE23_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBK6931051.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(23158..24576)
                     /locus_tag="LNE23_001183"
     CDS             complement(23158..24576)
                     /locus_tag="LNE23_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6931052.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(24633..25082)
                     /locus_tag="LNE23_001184"
     CDS             complement(24633..25082)
                     /locus_tag="LNE23_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6931053.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(25527..25790)
                     /locus_tag="LNE23_001185"
     CDS             complement(25527..25790)
                     /locus_tag="LNE23_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBK6931054.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(25889..27184)
                     /gene="rho"
                     /locus_tag="LNE23_001186"
     CDS             complement(25889..27184)
                     /gene="rho"
                     /locus_tag="LNE23_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBK6931055.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(27181..28452)
                     /locus_tag="LNE23_001187"
     CDS             complement(27181..28452)
                     /locus_tag="LNE23_001187"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK6931056.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(28617..29486)
                     /locus_tag="LNE23_001188"
     CDS             complement(28617..29486)
                     /locus_tag="LNE23_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBK6931057.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(29778..31388)
                     /locus_tag="LNE23_001189"
     CDS             complement(29778..31388)
                     /locus_tag="LNE23_001189"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBK6931058.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(32581..33921)
                     /gene="glnA"
                     /locus_tag="LNE23_001190"
     CDS             complement(32581..33921)
                     /gene="glnA"
                     /locus_tag="LNE23_001190"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBK6931059.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(33949..34341)
                     /locus_tag="LNE23_001191"
     CDS             complement(33949..34341)
                     /locus_tag="LNE23_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6931060.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT        10065 a         7256 c         6122 g        11109 t
ORIGIN      
        1 acaacctctt attccaaata aatggtattc aaaatttagt tctatttatt atcttgtctt
       61 attccgtata tcattcctcg ataatccgac aatataactg actcgtaatg acataaagca
      121 aaagatagat aaggatcaat aagccactca caagataagc caaccagtaa tcggcagttt
      181 ttatatactg agtgaagaca ttgattgcaa atactgcgtg agtgatccct aatatcatgg
      241 gtggaaagaa ggtaagtgcg ttttgctcga agatcatccg tctgatcctt ctttgtggaa
      301 tccccagctt agtcaatagg tgataatttt ctttttcttc ctctgcttct gctaattgcc
      361 ggactgtcaa aatactacca gtagcaataa tcacgatcat cccgacaaat agtgtgacaa
      421 aaatattgat accgattcgc cgattaactg cacgcaattt cgaatagctg gaagagaaat
      481 tcaaacgaga gctttcttgg ctaaattgtg cttccttcat ttggtcatcc ggaagtttct
      541 ttgtgctaat ttctccttgt agacttccat ttacgatatc ataagaataa taaacagcat
      601 tgagccactt tatcgacagg tcattgctca gtcgttcatc tattttttca ttatttccac
      661 ctgatacatc tacattgact acttgataat ccacaccttt tgctttttca aaaacggtat
      721 catctacaat cagtacagga cttagatata ccattgtttc gtcacctaat acacttggaa
      781 gcatttgttg aatcttcaat tcttgattat ctggcagtcc aatactagat ccatataatg
      841 aaatattccg aaaaagagtt tgcatttgat caagcaaaac agtatgacca ggtgatttca
      901 aagaaatgtt cggcaaatct ggattaactt ttctgaaggc ttgatattgt gtttccgtga
      961 tcagattagc agtttgaaac ataggttctt cgagattttg actatagata ctcccaatga
     1021 ctttataatg caaggtgatt tctttagtta tcttctgccc ttcctctgct agaatcggct
     1081 tgattttttt ctctgtctca acatcaaact gatagtccat cggggaggaa actttctcct
     1141 ggtaggataa aatagtagag atactagccg tgccgccaat tgtcattaaa gaaattccga
     1201 gaatgactgt aatcagagaa tttgtacgcc aacttttgag caaatgaact tgcgtgttac
     1261 ccattatcaa aaaattgata ccatagtaat aaatcttcca tttactcaat atgatcaaaa
     1321 ataaccgaaa tgtataacag aaaaacaaat aagtaccgac cacacaaacg aaaaaaatta
     1381 aaaaaggaaa aaagaaaact aatgtatcaa gataatgata actgatcatc catgaagtga
     1441 tttccctaaa atgactagct ccaaaatatc cgctcgttaa aagtagtaac ccgatgactg
     1501 cgagtaatcg gtgccttgtc ctgaagcgca ttgttgaact atctgcttgt tctttttctc
     1561 caaaacttcg aatcattgga tagcgccata ttttccatat tgaatataca gatactgcta
     1621 aaaggatgat gaaaaaagca aataaagtat ctactactga tggaatggag ataaaaaatg
     1681 gactagtaag atccatatcc atcattcgaa ccaaaatcat tgaaaagagt ttggaaaaaa
     1741 taataccgag taatatccca gaaatacagg cgaacaatcc aattatcatg atttctaaca
     1801 catatatacc tgatatctgc agtttattca taccaaaaag ctgatagatg cctatttcct
     1861 tttgccttct attcaagaaa aatcggttgg cactgaatac gaaaaaaagc aaaacaacag
     1921 taatgataaa tccaccaaaa ctcaatccac catctatcga agtatcttga cttgctcttc
     1981 tgactaaaga ttgatcgtag gtcattgcgc tgaaggaata atagatcatt acggataaag
     2041 tcaaactgaa aaaataaaca agatagcctc gtgactgccg aaaaaaactt ttaacggcta
     2101 gtttatacag cattcatttt tcctcctccg ccaatcgtcg cctgcatgtc tattactttc
     2161 tcaaagaact ctcttcttgt ccctcttctg acaacttcag aaaaaatcac accatcttta
     2221 ataaataaaa ttctatcgca gtagcttgct gtatatggat catgggtgac taacagtatc
     2281 gttgcttttt ctttttgatt catggtcttc aagtagttca acaattctgt agccgacttc
     2341 gagtccaaag agccagttgg ttcatctgca aaaatcactt gtggctgaac aacaagtgct
     2401 ctagcagctg caattcgttg tctctgtccg acagaaagtt catcgggata ccgtttgagc
     2461 agttcttcta tattcaacaa ttgagctaca tgtcttactc tcttttccat ttcctcttta
     2521 gtcatccgtt caactgctag gggcaatagg atattgtctt ttgcatttag ggtatcgatc
     2581 aaattgaact cttgaaaaat gaatcctagt ttttttcgac gaaagtcact gagctcactg
     2641 tttttcattt ttgtgatttc ttttccttgg atcgagacac tgcccatcgt aggcaaattg
     2701 atcgtggaca agatattcat cagcgttgtc tttcctgcac cacttgggcc cataatccca
     2761 acaaactcgc cttcttctac tgtgaatgag atattatcta aggctttgac gggagaaagt
     2821 ttgctgccgt agcttttact tagatgtttc acttcgatca tcttttccaa ttcttctcct
     2881 cctctttcat ttttcatttc tatttatttg catagtagca tatctacgaa taatttgctc
     2941 ttatgttttt gtcactttta cttggactga ataaaaaatt ttattcaatt attttattca
     3001 gcaaaattaa atatgcaaaa aacaaatcca ccagctacaa gttttcacct tttttcacaa
     3061 taaaatatac actacatata gtatagaaat ccaattatac agcacattga atactatata
     3121 tagccgatat ttagttgctc cttccccgat tttgttttat gatagacaag aacaatttag
     3181 caggagttga agataatgaa agtaaattat gaagaaaaaa ctgtcgttac tcagcagcca
     3241 gagacatccc agatgaaagt catcaaacgc gatggccgcc atgttgattt cgatgaacaa
     3301 aagatcaatg aagcactgat caaagcagaa aagaaaattc acggctcatt atcaccactt
     3361 acttatgaaa aaattcaaat gattgcagac ttggttgtcc aagaaatcaa aagccggttt
     3421 gctgaaaatg tcaaaatcta tgagattcaa aacatcgtgg aacatatatt actagaaaaa
     3481 aacgaatatg aattagcaga agagtacatc aattatcgta ccaaacgaga ttttgctcgc
     3541 agtaaagcaa cagatatcaa cttttcgatt gaaaagctaa tcaacaagga ccaaactgtc
     3601 gtgaacgaaa acgcaaataa agacagtaat gttttcaata cacagcgaga tctgacagcc
     3661 gggatcgttg gaaaatcaat cggactgaaa atgttgccac cacatatagc aaatgcgcat
     3721 caaaaaggcg atatccatta ccatgatttg gattatcatc cttatacacc gatgacgaat
     3781 tgctgcttga ttgatttcaa aggcatgctg aacaatgggt tcaaaatcgg caatgccgaa
     3841 gtcgaatcgc caaaatctat ccagaccgcc acagcacaaa tctcacagat tattgccaat
     3901 gtagcgtcaa gtcaatatgg tggctgttct gccgaccgaa cagatgaatt gcttgctcct
     3961 tttgcagaat tgaactataa aaaacatcta aaagacgcgg aagaatggat cgattcgcca
     4021 gaacgccaaa aagaatatgc aaaagcaaaa accaaaaaag atatcttcga tgctatgcaa
     4081 agtttggaat atgaaatcaa taccttattt acttcaaatg gacaaactcc tttcacttca
     4141 ttaggatttg gactaggaga aaactggttt gaaagagaaa ttcaaaaagc aattttacag
     4201 attcgaatca atggattagg gagtgaaaag cggacagcta tcttccctaa actgatcttc
     4261 actttgaaaa aaggtgtgaa cttaaaccct gaagatccta attacgacat caaacaacta
     4321 gcattggaat gtgcaacgaa aagaatgtat cctgatattc tgaattacga taaaatcgtt
     4381 gaattaactg gaagtttcaa agtacctatg ggctgtcgtt cattcttgca agggtggaaa
     4441 gacgaaaatg gtcaagaagt aaatgtcgga cgaatgaatt taggtgttgt tacattgaat
     4501 ttgccaagaa tcgcgatcga atcaaaagga gatcagaata aattctggca gttactgtct
     4561 gaccgcttag aaatcatgaa agatgcgctt ctctatcgtg tagaacgttg taaagaagcc
     4621 attccagcaa atgcgccaat tttatatatg tatggtgctt tcgggaaacg tctttcacgt
     4681 accgattctg tcaatgaatt attcaaaaac cgtcgagcta ctgtatcgtt aggctacatt
     4741 ggattgtatg aagttgcttc agctttcttc ggtggcgaat gggaaacaaa cccagaagca
     4801 aaagctttca cacttgatat cgtcaaagaa ttaaaagcta atgcagatgc atggggggat
     4861 gaatatggtt atcatttcag tgtctattca actccaagtg aaagtctaac tgaccgtttc
     4921 tgccgtttgg atacagaaaa attcggcatc atcgaaaata tcacagataa agaatattat
     4981 acaaacagtt tccactatga tgtgcgtaaa aacccaacgc catttgaaaa attggaattt
     5041 gagaaggatt atccaaaata ttgttcaggt ggattcatcc actactgtga ataccctatg
     5101 ctccaacaaa atccgaaagc tttagaagct gtatgggatt ttgcatatga caaaatcggg
     5161 tatcttggta cgaatacgcc aatcgatcat tgctatcaat gtggtttcga aggtgatttc
     5221 aaaccgacag aacgagggtt cgaatgtcca gaatgtggaa atcatgatcc aaaaacatgt
     5281 gatgtagtca aacgtacttg tggctattta ggaaatccgc aagctcgtcc gatggtacat
     5341 ggccgccata aagaaatttc ctcacgtgtg aaacacatga aatgaggttg tgaaaaagct
     5401 gtcctgcatc aagtaataag aatagctaaa caaaaatagt tcctcatatt tttcgtttag
     5461 ttgggcttat tactgcagat gcaagctttt gaacaccgtt tattcagaat aagaggctat
     5521 gacaagtctt ttttcaccac cctctttcga tggctttgta tctttgaacg ccctctatca
     5581 aaataagaac aagctttctt gaacaatgga tttaggtcag tttgttctta tttatgtggt
     5641 cgagtagatt ttatcttcaa tagctatatc tttgaacctt aaatgaaaga agttttttta
     5701 tgagaaatcc aaaaccaaaa gaatggttag caaaagacta cagccaaaac tatattgctg
     5761 actacaaacc gtttaatttt gtcgatggtg aaggtgtccg caacagtctt tacgttagtg
     5821 gctgcttatt tgcctgcgaa ggctgcttca acaaagctgc acaaaatttc cgatatggca
     5881 aaccattcac acctgaatta gaagaacaaa tcatcgctga cttgcgcaac gattacgtgc
     5941 aaggattaac tttacttggt ggcgaacctt ttttgaatac agatgtttgt ctgcaagtcg
     6001 tacgacgtat ccgaaaagag ttcggtacga caaaagatat ctggtcatgg acaggctata
     6061 cctttgatga attgctacaa gattcagaag ataaattaga actcctttcg caaatcgata
     6121 tccttgttga cggacgcttt gaattaagca aacgtgacct aaaactacaa tttcgcggaa
     6181 gtagcaacca gcggatcatt gacgtccaaa aatctttaga atccaatcaa gttgtgattt
     6241 gggaaaaatg tacggatgca acagaaacct atgaacaaat caaaaagcaa gacttgatat
     6301 aaaaacagct ttgggagaga aaacggattt ccggtttcaa ctctcaaagc tgtttttata
     6361 tttcttgatt ttcccataat ggaaggacga tgttttcata agtcatcggg tccagattcg
     6421 tcaaagtaat ccctaacaat cggattcctt tttctactcc caagatttcc tcccatataa
     6481 ggcttgcttg ataaaagagt gcttcttttt tatagatata ttctggcaat gtcactcttt
     6541 tggtcacagt agaatagtcc gtgtacctga ccttcaaaac tactgttttc ccatgttttt
     6601 ggactcttct cagtgcttct tctacttttt cagctagttg gcgcaactga gtcagcactg
     6661 cttcttccgt ttgcaacggc tgcccataag tatgctcttt tcccacagat tttcgctctc
     6721 ttgtaacgtt tactggagaa tcatggattc ctctgacttt acgataaaga gaatagccca
     6781 tttttccaaa atttcgaatc aacatcattt ctgtgcattc atataggtct tttcctgtat
     6841 aaatccctag ctcatgcatt ctaggtacag ttttcttccc taccccatga aatttttcaa
     6901 ttggcaaagc ttttaaaaat ggcacagcgt cttgaggggc aactaccgtg attcctctag
     6961 gtttttgata gtctgatgct aatttggcca aaaacttatt gtaacttact cccgcagaac
     7021 atgttagctg gacactctcc caaatatctg cttggatcat tcgggcgatt ttgatggctg
     7081 acttgcaatt gattttattt tcggtcacat caagatacgc ttcatcgata ctaactggtt
     7141 ccacaatatc tgtataacga tagaatactt cccgtacttg tttggaaata tcgctgtatt
     7201 tatgatggtc gccaggctta aagatggctt gcggacaaag ctcatacgct ttttgtgcac
     7261 tcattgctga atgaattccg aattttcttg cttcataatt tgctgtagtc actactccac
     7321 gcccaccagt gtcactgggg tgtctggcaa ttactaatgg atgtccgcga aactcaggat
     7381 gatctcgttc ttcaaccgaa gcgaaaaaag catccatatc aatatgaatg atttttcttg
     7441 atgtatcatt atgtaaagga aattgcagct ccatcatcct cacctcttat tcattataca
     7501 agaacattcg ttcgcgttca agagatttga aaagttagta gctgatttcc tcattagtct
     7561 ttcattatag acaacttata ctatctcagt ctaattcatg gtactgacgg tagacttctt
     7621 gttttttcag cccatttttc agcgctactg ctttgatagc agcatttggt ttttcgcctg
     7681 aagcgatctt tgcttctact tgttccttta acgttcccaa ctcttcatca acttccgtca
     7741 ctggcatttc gaacaaattc ccttctacaa gcagacaaca ttcacccttt actggatgtt
     7801 cttcgatata agcgagtagc tcttccgtac ttcctctaag gtattcttca tggacctttg
     7861 tcaattcccg acacaaaacg acttggcgat ttccaaaaat ttctaagaaa ttcgtcaatg
     7921 tagcagtgat ccgataaggt gactcataaa aaatcagcgt aactgtctgt tcttttagct
     7981 ttttcaatgt ttctttctgt tcttttttct tacgtggaag gaagccataa aataaaaatg
     8041 gttgaggcac tagccctgaa gcaatcaaag ccgtcatccc agctgtaggt cctgggatag
     8101 agatcacaga aatcccttct tttatgcagg ctaaaactag ctcatgtcca ggatcgctga
     8161 tggaaggcat tcctgcgtca ctgacttgag caatcgtttt tccttctttt aacaaagcaa
     8221 tcaattgagg gacacgttct ttataattat gttcatgtaa acttttttgg ggtgtatgga
     8281 tttcaaaatg attcaataac ttttgtgtat tacgcgtatc ctcacttgcg ataagatcgg
     8341 attcttgcag catacgaaca cttctgtagc tcatatcttc taaatttcca attggcgtcg
     8401 gtacaagata taaatcccca tataccggat ttcccttgaa acttttttga ttatacatct
     8461 gtcacaatct ccttaataaa aagactggag cagtatttct ccgctccagt ctcctcttgt
     8521 tattggtcat cgaccgcttt cgcgatagat cacttctaga caaaacgcac aaggctcgtc
     8581 attgtccctt cttgatccat ataaatcgta gcagacatgg aatccttctt cataaatttt
     8641 ttcaagattc atacgagact tagaaagttc ctgcttcaca ttttcagctg gagcttgtgc
     8701 aacttggttc aattcccgca ggtgttcccg cagacgttga ttttccatct ccaaagttgt
     8761 atttttttct acgatctcat gcaaagcatg cttcatttca acaagttctg ttaatgtatt
     8821 tctcaactct gattctaatt gattcaatcc gtcgtataat gatctcttat ccattcatct
     8881 cacctacttg gataaattct ttcaattcat cccattcata ttcgacagga ttttcccgtc
     8941 cagtcagccg aatactaacg atacggctaa gcagattcaa ccctacgact ttccctttac
     9001 catcaggtgt agcaattact tttccaaagt cagggagttc ttttttcgct gtctcgtatt
     9061 cttcgctctc atattgcaga cagcacatca agcgtccgca taaaccggaa atttttgttg
     9121 gattcaaaga taaactttga tcttttgcca ttttgattga tacaggcacg aaatcgccta
     9181 aaaaagaaga acagcataat ggacgcccac atggaccaat tccgcctatt atctttgctt
     9241 catcgcggac accaatttgt cgaagctcaa tacgagtacg aaatacagcg gccagatctt
     9301 ttaccaattc tctaaagtca attcgcccgt ccgctgtgaa agaaaaaatc aatttatttc
     9361 gatcaaatgt gtattcggct ttgaccattt tcatttccaa ctcacgcaac tgcacttttt
     9421 cttttgcgat tttcaatgca tcataagcat cttttttgtt tttttgtctt tttctaattc
     9481 agcttgtgac gctttatgca agatcggacg gatagcttca ggaaggtcat tgggatcaat
     9541 ctctttttta ggaaggacaa cagtagcaat atgtaaggat tgctgagatt cgactaatac
     9601 cttgtcacta taaacatatt cagagtctcc tggtgcaaaa taatagatat gacctgcatc
     9661 tctatatcgg acacctacta cttctaccat ttccatcctc cttgatcatc atcctttgcg
     9721 gcaagctaaa aaatcgtatg caaaacgaac tgttccgcaa cagcctgaaa actgacattg
     9781 ctgcgtagtt tctgctctgc gatcaatatc cgttcaatta tctggtttat ctgtttcact
     9841 ttgccatatt cgccgtttgc tactgcttga cgctgtgctt cctgataata aaacatcaag
     9901 acagataatc ctgtaaactg ttgtgttttt tctttaaatg attttaccaa ttttttttgg
     9961 acatagataa aggcttgagt atcttgcttt tgcaagtaaa cgaaccattg ataaatcaag
    10021 tccttagcat cattaaacca ttcatcttga gagatttcaa ctgcttttcc taaactgttt
    10081 gttaagaaag ccaaaagttt tgctttttcc aatggaatct gctcttcttg caatcgatgg
    10141 atcagtgctt ctttcgaaag ttcttgaaaa tgcagaactt gacaacgaga ttgaatagta
    10201 gggagaattc ttcctactgc atctgtttct aacaccgcta aaaaatctcc agggggctct
    10261 tccaagaatt tcaacaaact gtttgccgca ctgctattca ttttttcagc ttctttgatc
    10321 aaaaaaactt tttttcttga ttcgtaaccg ctgcgactga attccgtctg caaacgtctg
    10381 atctgatcga ctttgatggt ttgtccttcc ggttcaataa ccaaaacatc tggatgttct
    10441 ttcttttcga tccgctgaca attgttgcat tttccacaag gcagattgtc tttcatttgg
    10501 gtacagaaca ggtgctgtgc aagccatata cctacttcat gttttcctgt tcctttctcc
    10561 ccttcaaaaa gataagcatg agcgatacgc ccatgctcaa aactccgttg aagctgctga
    10621 tacacgatcg gttgcatttg agccaagttt atttcttgat tcatagctca gctcctccta
    10681 aaattgatgg aatccttcaa caggtaagac gaagactgtg gcaccgccta cttctacttc
    10741 tactggataa gggacgcccc catccatcga aatatccagt gtaacaggtg tagatacaaa
    10801 ttgctttctt gattcacacg tttttttgat gatttccaat gcttcttcta cacggtcatc
    10861 ttcaataccc acgataaacg tactgtttcc agccttaagg aagccgcctg tcgatgatag
    10921 ttttgtggca cggatatttg catcaatcaa ttcatttgat aaacggttgc tgtctttgtc
    10981 ttggataatc gctaaaataa ttttcataaa cgttcacctt cctagttttc aaaaaattgc
    11041 ggatactgtt caataattgt atgataactt gtttgcagca cttcttcaaa gctttttctt
    11101 gcatcaactt tgtggattct ttccggattc tcttctgcca gctttaagta agcatgacgt
    11161 acacgctggt ggaattccaa accttccgaa tctaaacggt cgatttgatt ttgtctgttt
    11221 ttcttgattc ttcttaatcc ggtgtctgaa tctacgtcaa gataaagggt aaaatccggc
    11281 gtagtacctt ctgtagcaaa ctcattcaac cgagcaatct cggtaacgcc gatgcgtcgg
    11341 cctgcacctt gataagcaag agaactgtcg acaaatctat cgcataaaac gattttccct
    11401 tttgccagag caggcagaac cttttcgact agatgttgtc ttcttgctgc tgcataaagc
    11461 agtgcttccg tacgttcatc catccgatca ttttttggat caaggatcac tgctcggatc
    11521 tcttcagcaa tcggaatgcc tcctggttct cttgtttgaa tgatttctgt ttttgctact
    11581 tctttcaaaa gagggtaaag ttcattcaag atgctggttt ttcctgcacc atcaggcccc
    11641 tcgattgtaa taaatagtcc gttcacattg ttgcccccat ctcttgtgct gcttttatat
    11701 tgtatctgaa aaaacactta attagaaatt tataactcac gtcttccttc gacagctttc
    11761 aataaagtaa cttcatctgc atactcgata tcagagccta cagacagacc atgtgctaga
    11821 cgagtcactt tgatacctgc gggtttgatc agacgtgaga gatacatagc tgttgcttct
    11881 ccctctgttg ttgcatttgt tgcgataatc acttctttta catctgcatc atgcagccgc
    11941 tgcaacagag aagaaatatt gatgtcttct ggacctgtcc cttccatcgg tgataacaca
    12001 ccatggagaa catgataaag tccgtgatat tctcgcattt tttccattgc cattacatct
    12061 ttcggctctt cgaccactag gataacgctt cgatcacgtg aaggatttct acatatttcg
    12121 cagggatctt cttctgtgat gtttccgcag atactacaaa aatgcaaatc gcgttttaca
    12181 cttaacaaag atttagcaaa ttcatttact acttcatctt tcatatcgat cgtataaaag
    12241 gcgagtcttg tcgctgtttt ctgaccgatt cctggaagtt tcatgtagct gtcgatcagt
    12301 ttcgcaattg gttctggata ttgcatattc attactcctt cagtttaaaa ctaaaatcct
    12361 ggcaaacctt ttgcgtattt ccctaatttt ttctcagttt ccacatcaat ttgtgcgatc
    12421 gcatcattga ctgccattac gacaagatcc tgaagcatct cgatatcatc aggatcaacg
    12481 acttcttcat tgatttggat atctttcatg cgacgatcgc ctgtaaaaac cgctttgacc
    12541 aattcattgg tggctttacc tgtaaactct gttttattta gcagctcttg tgcttctgcc
    12601 atttcttttt gcattttttg tacttgtttc atcatgcctt gcatattgcc cattccacgc
    12661 atcataatta tctactcctt tttattaatc atctaccact gtaacagctt cggagccaaa
    12721 cagttcctct gcttttacta caagtgtttc ttctggtact tcttgttctt gaggaagcaa
    12781 ctcgatttct ccttctgagt cttcagaagc aaaagagttt tctgttccct cttcttgtgc
    12841 ctgcgtcaaa taatttcttc gaagttctgg ccagctttct cttgtaatat aaacaggatc
    12901 aggcgcataa tctttgatca ttcggcttaa gttgttgtgt accgaaagct gcaattcttc
    12961 gtcatttgct gctctttgac aaacaatctc ataatcaaac gcaatcacta gtcctgtagg
    13021 tccagctgca actggttcgc ttgctttaag cattgctcgc tgagtgacag atagactcat
    13081 cagcaagtct tcccatactt cttttacttg gttcagatgc tgtcttgtcg cttcccgcag
    13141 aactttatat actcgttccg tcggtacacg ataggtactt gtctgttttt tatgatttgt
    13201 ttgctgtacc ggctgtactt ttgtattctc aattcctgta ttactgcgta gttccttgat
    13261 ttcgtttctt agttccttaa tctcttgttg tagctgtttt actatctgat catctgcaaa
    13321 tgctacatct gctgaaggaa cagaagcaga cggtagctgt gcaagtttga ctgtagcgac
    13381 ttccaaataa atagcggcac tattggtaaa acgaatttca ttttgagtat cgctcaaaat
    13441 ctttatcatt gaaaacagct gatccgctga aacagtttgt gccaattctg taaatgtact
    13501 tctgatcgta ctcgattgtt cctcaactag gttaggtgct tgctgataca tcagcaagtc
    13561 acggcagtat tgtaacatat cctctagaaa gcgacgtgat tctttgccgg ctgcgagaat
    13621 ttggttcaat gtttccaatg cttctggtac ttcttttttc acgcaagcac tcaagtaact
    13681 gtccatcatt tcgtcagtta agctacctgt cacttgcatg gcatcatcta aagtaattgt
    13741 tccttcacta aacgagattg cctgatcaag aatactcaat gcatcccgca tcccgccttc
    13801 tgcagaacgg gcaatcacat tcaatgcagc tggttcatag ctgatagtcg attctttcaa
    13861 gataaattca agatgttcga caatatctgc cgtattgatc cgtttaaaat caaatcgctg
    13921 agtccgtgaa atgatcgtag ctggtatttt gtgaggttcg gtcgttgcta agatgaatat
    13981 gacactttct tttggctctt ccaatgtttt caacaaggca ttgaaagctc ctgttgaaag
    14041 catatgtacc tcatcaataa tgtacacttt gtacgctgcc tttgtaggcg cataatttgc
    14101 ccgatcacgg ataaatcgga tttcttcgac tccgttattg ctggcagcat cgatttcgat
    14161 cacatcttct tgcgtaccag cagtaattga tcgacacatt tcgcattcgt tgcatggctc
    14221 accgtcttta ctgttcggac agttgattgc ttttgcgaaa atctttgctg cactcgtctt
    14281 tccggttcct ctgggtcctg taaacaagta ggcatgtgag gttttgtggg aaacaatagc
    14341 attttttagt gtctgtgtca cagctttttg tccaacgatg tcttcgaaac gctgcgaacg
    14401 ccagactcga taaagtgctt gatatgccat aaggatcccc cttttttaaa aaattcacta
    14461 tctcttatta tacgtgatat taggtaaaaa aactactatc cttctcggct ttttcaggta
    14521 tttaaacaat acgcaatttt ccatttcaat tccgtctcta gtccgagaag tttcacatct
    14581 tcctatggta taataaataa acaataattg tttggcacta gctgaatcag aggaggaatt
    14641 ttttatgggt cttatcaaag cagcaacaag tatggttggc ggcggtctgg cagatcaatg
    14701 gctagaagtc atcgaaccag acaatatgag tgatacaacg gtcatgacaa aaggtgtcaa
    14761 agtccgcaaa gatgacaaac gtggctccaa tcgtaaagga acagaagatg ttttaacaga
    14821 cggaacagtc gtccatgtct atccaaacat gatgatgctt ttggtcgatg gtggaaaaat
    14881 catcgattat accgctgagg aaggttatta caccattaaa aatgacgcag ctccttccat
    14941 gttcaatgga acactgaaag aagcaattgc tgaaactttt gaccgattca aatttggcgg
    15001 cgtcacaccg cagaaacaac aagtttttta tatcaatctc caagaaataa agggaatcaa
    15061 attcggtaca agcgcgccgt tgaactactt tgacaatttc tataacgcag aattattctt
    15121 acgggcacat ggcacttatt ctattcatgt cgttgacccg attttatttt atacaaacgc
    15181 aatccctaaa aacaaaacac aagtggaaat caatgatatc aatgagcagt atttagctga
    15241 attcctaaca gcgctacaat cagcaatcaa tcagatgtct gcagatggtc aacggatttc
    15301 ttatgtacca tctaagagtt tggaattaag caaatacatg gatactgcat tggatgattc
    15361 ttggcgtgaa ttacgtggga tggagattgt ttctgtggct gtagccagca tttcttacac
    15421 cgatgattct gtgaaactga tcaatatgcg taacgaaggg gcgatgctag gtgatcctag
    15481 cgtacgtgaa ggatacgtgc aaggttccat tgcacgtggt atggaagcag ccggaaaaaa
    15541 tgaagcagga gcaatgaccg gctttatggg tgtaggtatg ggaatgaatg caaatggttc
    15601 ttatctttct caagcagctc aaaataacca agaacaaatc aaacaacaag cagaaaaaca
    15661 aaatcaacaa actgcacaag gaaatgcgga tacttggaca tgtccagttt gcgggacaga
    15721 aaattccgga aaattctgtt ccaactgcgg agctgccaaa cctgtagaaa acgttcagcc
    15781 taagctacaa atgcgatgca gtgaatgcaa tgagatcgtg gatctttcca acggcatccc
    15841 taaattctgt ccaaactgtg gtaaaccgtt taaaggaatc cccgttaatt aatttattta
    15901 gaaaggtgtg cagctgatgg atgttcttac acataaatgt cctaattgcg gcggtccgtt
    15961 gacttttgat cccaaagacc aaaaatttca ttgcgaatac tgcctgaata tctatacaga
    16021 agaagaagtc agccaatatg agcaaaagca aaaagaagcg cgtatggctg gcgaagaaag
    16081 caaagaagaa ccttcaccta ccgaagattt tacttttaca gcccaagaac agcttgccga
    16141 tatgaatgaa gccgagcgca aagcggttga cgaggcaggc ggtttttcag atacagcgga
    16201 cgaatctgca gataataccg cagcgataga tctttttctt tgtcccaact gtggtgcaga
    16261 gatcgtgact gatgcgacaa ctgctgcgac ttattgttac tactgccata atcccgtcgt
    16321 actatcggga cgtttaagcg gagaattctt gccaaataaa gtacttcctt ttgctgtcga
    16381 gaaagaagaa gcaatcaaca gatttctcgc ttggaccaag aaaaaatggt ttgttcccaa
    16441 agcttttttc aataaggatc aaatcgataa gttgacaggt gtgtatttcc cttattgggc
    16501 tacagatgct gaagtagatg gtgccatgca ggcgaatggt acagttatcc gcatatggcg
    16561 gataggtgac atcgaataca cagaaacgaa gcaatttgct gtttcaagag caggcaagct
    16621 ttcttttaaa gaactagtaa aaaatgcctt atcaaaaaat acgcaacaaa agatggtcga
    16681 aggcgtgcaa cctttcccgt tagataaagc aattgatttt aaaagccagt atttagccgg
    16741 gttccaagca gaaaaaagag atatcgaata tgaggcaatc aaatcacaga ttcaatctga
    16801 attaaaagac tattcagaga agctgttaaa agataccgca aatggatata caacattgac
    16861 cggtgtgcga acttctgctt caatcacaaa cgaagtaaat aattacgtat tactgccggt
    16921 atggcttgtt acttatcgaa gcaatgactc aagtaaaaaa gtctactact acgcaatgaa
    16981 tggacaaaca ggaaaggtca gcggtgtcct gccgatcagc cagaaaaaat tgggactgac
    17041 cgctcttggg attttcgctg ggctagcgat cctctttctg atcggaggtt acttgatatg
    17101 atgaaaaaat tttttttatt ctttcttttg ctagtaagtg ttggctggcc ggtggctact
    17161 catgcagata cagctacggt aaatgatgag gctgggttat tcacaacaga tcaaatccaa
    17221 tctttggaac aacaggcaga aaagctgaat gaaaaaatca aaggacgagt cttgatcgta
    17281 acgactacat ctaattcaga agaaccaaga gactttgctg ataattactt aagagacgca
    17341 gtcggaaatg atcaaaatgg ctcagtattg ctattagata tgggacagcg tgaaatctac
    17401 atttcgactt ctggtaatat gatcgattat ttgactgata gcagaatcga ttcgatctta
    17461 gatgatgttt atgatcagat gacaaatgct gcctattaca atgctgcaaa agcttacctg
    17521 tctaaatcag caacatatgt ggaagacgga gttccaggag gacattatcg tgtagacgaa
    17581 gaaactggca aaattacacg ctataaggtt ttgacgacag tagaaatcat cattgctgtt
    17641 gttttggctc ttatccttag tcttgtcttt tattttgtga ctgtttcgcg ttatcaattg
    17701 aaatcaggca tctacaaata tccattccga gaaaaatcca gtatcaaatt aactgaaaaa
    17761 accgatcgtc tgacaaattc ttttgtcacg acaagaagaa tccccaaaag cccacctcct
    17821 ggaagcggcg gtggcggaag tactacccat tctagcggtg gcgggacttt tggcggtggc
    17881 ggcagaagtt tctaaaaatt gctaaatgaa tgttcgcaaa aaaagggctg tgacaaaaat
    17941 cgtgtcacag ccccttttgt tgcaaataaa cggcgttcaa aagcttacat ctacggtgat
    18001 aagtccagcc aagttaaaaa cataagagac tgtttttact tggctcttct tattacttga
    18061 tgcagaacag ctttttcaca acctcttggt aaacggtgtt ctcctaccaa ctcctcggtc
    18121 acaagtcaca atattcatca aacatgaaaa gctgtttttt gaaattgttc cttgtgactc
    18181 agagttaaac ggcaggctca caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg
    18241 tattagctca aaaaacggca aaatatgagg aactattttt ccgtttgttt ctctaatact
    18301 caggtcaaaa cgctgatttc acaacctctt tgttcatcaa aagataatat tgtcttctaa
    18361 caagtccata ttcaaaataa cgatatgatg gtctttaacg gtgataatac catctttttt
    18421 cagttgtttt aaaattctgt ttacactgct agcagttgag atgccacaaa atttggcgat
    18481 ttcttcattc gtgatcgaaa aatcgatcag catcccttct tctgtccgca caccgaataa
    18541 atcatataat tcgtaaagtt gcgtacaaac agctcccagc tttccgttta atagcatttg
    18601 ctgcattttt ttcatcgaat aaagcagacg atagcggtaa tactctttta catagtgttg
    18661 tagctttggg tcttggttga tattttgcca aaagcgcaca cgattgattc gataaaactc
    18721 tgcatattct gattcaactc gaatattata cggcgaatcg atgaattgtg aatattcgtc
    18781 ttttaaaaga gagacgattt caagatcttt gatatagcga aggttaaatt ctctcccatc
    18841 atttgaaata acacttgctt ttaccgttcc ctctttcaag atatacgtat gggaatcttc
    18901 catcccttcg aatgtcaaat atttatgata tcctttgcgt ataatcggaa aatcattttt
    18961 atccaaatat tcctttaaga ctctactatc caatgatgct cccccatcca aactttcatc
    19021 taaaaagatc atacagtact ttaaaacttt ttaaagtcca aaggtatttt actaatgata
    19081 ttaacatttg ttaagtacct ttttccatca cttatgcgat ttttttcctt ttttttattc
    19141 gttatactgc agaagtaaaa ttttttaaga atagagggaa aaaacatgaa ttactggatg
    19201 aacacaatca ttaatcgatt agaaacagca tatcaaacaa gatttgatat gaaagcttct
    19261 ttagtctttt taaatgatgc gtatcaaaac tcgatcgaat tgatcaaagc agtggatgag
    19321 aatccaacaa acgaatgcga agaattttta aatctgttta tgtctacaag agatttattt
    19381 attcgccaac ttgttgatcg ctatccatcc aattatcacg atgtagaagt tcaaatacaa
    19441 aaattaaaag catattcagc ttaaggggaa tgtataacat acatggaaaa gaaattattt
    19501 aataaatatt ttattggaat cacattgatc aattttattg tctacctgat ttattatctg
    19561 ttgatggtca tcatggcagt cattgcgcaa gacaccttac acgccagctt gagccaagca
    19621 ggttttgctt ctgggatcta tatcatcgga acattgctgg cacgcttatt catggggaaa
    19681 cagttagaac tatttggtcg gaagtttacc ttaagaggcg gcgcgatctt ctatctcctt
    19741 tcaacattag catatcttta tacgccaact atcggggcat tgtatttagt tcgattcttg
    19801 aatggattcg gctacggtac agtttctaca gctacaaatg ctatcgttac tgcatatatc
    19861 cctgcatcta aaaaagggga aggcatcaat tattatggat taagtactag tttagctgcc
    19921 gctgttgggc catttttagg aatgattttg ctgaatatga ctgattttcg tttcattatc
    19981 tggttttcaa ttgtactggt cttctttgtt acgattgcct gccttgtctt tcctgtaaaa
    20041 aatattccat tatctgaaga acaaaaaata tctcttcgct catggaatat caatagtttt
    20101 attgaaaaga aagcactttt cattacagga attgcctttt tgatgggtct ttcttattca
    20161 agtgttttat catttttatc ttcttataca aaagtaatcc acttagttgc tgctgggtca
    20221 tttttcttcg tcgtatatgc tttggtcatc acatttacac gtccattgac tggaaggatt
    20281 ttcgatgtca aaggagaaca atacgtgatg tatcctagct atttattctt aacagcagga
    20341 ttatttttac tgagtgtaac gacgaatagt gtgacattgc ttatctctgg agcattagtc
    20401 ggcctaggtt atggtacgtt tatgtcaaat ggacaggctg tttgtttaaa aatcgtccgc
    20461 gaacatcgaa tcagtattgc tttatctacc tactttattg gtttagacct aggcttaggc
    20521 gttggtcctt atttgatggg gatgctaaaa tctcttactt catttagagg attgtatgtg
    20581 attgccggag tcattccatt gatatgtaca gcactctatc ttgttaatgg ccgaaaacat
    20641 agtcatgaag cagagggcga attgattgaa tcattgaaaa atgaaatttg atcttgatga
    20701 atagataaaa aaaaccgttc agatctgaaa ataatctgag cggttttttg agtaaaaata
    20761 gtttatgatt attcttaatt aaacatctat ttatttttaa agctttcatt tatcgataag
    20821 aatagtttaa ctgttgattt attccatttt ccgttcatct ggtacgatgc cttgacttag
    20881 caaccacttg tggaagaacc attctgccac accgatcaat agagcaggaa tcaatacttt
    20941 catccaaata tcgactgtat cattcataaa atatagatag agccacaaga taactgcagc
    21001 tgaaccagca tctgcgatcg tcgctgcgat gttccctact ttacgtaaga tcaacagatc
    21061 ccctagcaca tagatcatga ctacaataat taccccaaca atcgttgaat ccataaagct
    21121 aacaccgtat aaacctgtca gcacgaccca cagaatcgcc cagatcatga ttgccttcac
    21181 taccaacgcc ttcacatgtt tcattttctg cactcctttc tcctggataa aaaagtaaga
    21241 ttatctaaca agataacctt ttcattatta taccatcggc tgagagaagc gagcaaagat
    21301 aaggtcctat ctaaaaatga ggaagccact tagaacattc acgtaataat ttatccgtac
    21361 agacattcga tatggtgttc ctttactttc gcatactgtt cttttgtcag ttctttactt
    21421 gtcaccagat aatcgatttc atgaaaagca atggcttgat gcatgaattt atgtgcaatt
    21481 ttttgctgtg taactaaaac ggcaagccgg tctgattgtt ttctaaaagc ttttttgacc
    21541 attgcctctc cttcatgagt ttctgttact ccttggctga aatctagccc gcgacaactc
    21601 attatcgcta aatccgcata atatgacaat gcatcgttat aggcttttga accgttgatt
    21661 gttgctcgtt tcggaacaat tgcccctccc aatacagata cttttgccgt tgtatattca
    21721 ctcaacatag cagctgtgac gatgccattc gtaatgatct gcaaaccatc aaatcttgct
    21781 aggtttctga ctaagtgtaa agaggttgta ctggaatcca agaataagac catatcatct
    21841 cgaatcaaag aagaagctgt atctgcaatc ttacgttttt ctgtttcttc ttctaatttg
    21901 gtgatcttca tcaggtaagg cacgtcaatt tttcgattgt caataagcat ggcgccaccg
    21961 cgaactctac ggatcaatcc acttttttcc agaaaaacaa ggtcacgttt aatcgtggct
    22021 tcgctataat ttactaactc gatcaaatcg gtgactcttg caaattggtt gttttttaga
    22081 taattcacaa tctcctgctg tctattctcc acttcattca acataaaatc accccattta
    22141 ctttatatag taacaggaaa aaagaggttg tgacagaagt gattggcttc gagaaataag
    22201 aagaaatttt tcgaaaaaac tacttctgaa catcgtgtgt ggttaagagg gtatgacaag
    22261 actattgtca taccctcttg accacacatt tttattttta gtataaacta gccttgccta
    22321 ctgtgttcaa aatttccatt ttatgtttga ccacttcttg taccttttta ttagctgctg
    22381 gataaattgc attcggctcg tacatcgatg gattttcact caatatttct ctcaaagcat
    22441 tgaaaaagac gcttttaaga tctgagctca aattcacttt ggcaactccg tattttactg
    22501 cttcgctgac ttctgcgtcc ggattacctg aaccgccatg taaaacaaat ggaagatcga
    22561 tttgttggtt caattctttc agtaagtcga tatttaattt tggagttctg tttttcggat
    22621 acaagccatg cgctgttcca attgctacag caagtgtatc gattcctgtt tctttcacaa
    22681 aaatcttcgc ttgatcagga tctgtataaa tgattgtttc agcacctcct tcagcagaac
    22741 cattattccc aatcgttcca agctcagcct ccactgagac atttaccgca tgagctaacg
    22801 caaccacttt tttcgtaatc tgtacattgt cgtcgaatga tttcaaagac gcatcgatca
    22861 tcacggaaga atagccgttt cgaatcgccc gtaagacatc ttctatcgta ccgccatgat
    22921 ccaagtggat aacgatcggc agtttacttg tataagcata ggctcttact gccgcaacaa
    22981 actcatcact taaataggcg atttcatcag gatgaatttc taagattact ggtgcttcta
    23041 atctctctgc ttcttcgatg attgctttca tcatatcata gctgcaaata ttgaaggctg
    23101 gtactgcaaa ttgattctct ttagccactt tcaataactc tttcattgta tataacatta
    23161 cgcttccacc tcatttgtta gattttcatt tttcgtccac ttgtcttgtt ttttctcttt
    23221 cactgttttt ttcaatagtc caagcataaa ggctgtagtg actgtacctg atgcaattgc
    23281 caagacatac ataggtaaat tgttcataac atttggaatt agcatcaccc aaatccctcc
    23341 atgaggtgcc attgaagtaa ctcctactgc catggaaatc cctgcagcga ctgctgaacc
    23401 tgtcatcaaa ctaggtatca cgcggatcgg gtcagatacc gcaaacggta tagcaccttc
    23461 tgtaatatac gaagcgccta aaatccagca tgattgacct gaagtccgtt cttctttggt
    23521 aaatttatgt ttgaataata aagtggctaa agcaatacct aggggaggtg tcattcctgc
    23581 agccatacaa gcagcaatcg gtgcatttac tccagctgac atcaaaccga tcgagaaagt
    23641 cgaaatcgat ttattgatcg gcccacccat atcagctgcc atcatgatcc cgataagtaa
    23701 tccgaacaat agcccggatg tatttcccaa gctattcagc caatttgtca aagcatccaa
    23761 cacccatgtg atcggttgtt caatcacaaa taccatcaaa agcccaataa ttaagatcga
    23821 taaaaagggg acgacaatca gctgataaat agcagaaaat tcttttttta cgctaatttt
    23881 atttgccaga aaatcacaga tataccctgc tagcacgcca ccaatcattc cgccaagaaa
    23941 acctgaacca cctgtagagg ccagtaatcc ggcaacaatt cctggggcaa aacctgcttt
    24001 tccagcaatc gatgtagcaa tccccgcgcc taacgcagga accatcatgg caaacgctgt
    24061 atctcctccg atccttgaaa atgcagctgc taacacatta tagtctgctg agttgggatc
    24121 agccgctgta atcccaaagg caaaactcag tgcgatgatg attccgcctg cgatcacaaa
    24181 tggaagcatg taattcacac cattcatgaa atgattatag attgtcggct ttttctcatt
    24241 tgcagctggt tctttttcat tagacacaac gaatacgcca atatctctca atgcttcgtc
    24301 tattactact tgcggttctt tgattgctcg gcttgtagag acacgcttta cttttttccc
    24361 ggcaaatcgg tccaattcga tttctttatc tactgcaaga atgattgcat ctgcctcttt
    24421 gatttcttgt tcagataact gattttctgt ttttacacct tgtgtttcga attttgcttg
    24481 atggtttaga tttcttgctg ttacttctaa tttttctgcc gacatataag tatgagcaat
    24541 tccagtcggg caagcagtta caccaacgat tttcatgatt attttcctcc tttttcttca
    24601 ctatcgatac tcggacaaag actctactat tttcattctt gactaatctg tctgatgata
    24661 tctgctggcg tagtggcttc ttttattccc tttctaaaat ctgcattcat caatcgtctg
    24721 gagagtgtgc tgagaatctc tagatgcata tctgctgatt tttcaggaac tacgatggca
    24781 aaaatatatt caattggcat ttcatctatc ccgctatagt ccgttaattt ggctgtatga
    24841 aaaaaacaca gtgctggttt tgtcacatat ttgctttttg catggggaat cccaaatcca
    24901 tcttctattc ctgtggagat ctcctcctct cgtttgaata gtgccttaac gactttttga
    24961 acatttctta ccttatttgt ttttttcaac acatccccta ttcgctcaaa taatgatcct
    25021 tttgaatcta tcgatggatc gaaaacaatc agttcttctg cgatcaattg atcaaacctc
    25081 ataaaaaacc tctcccccac aaaaaatgtt aacgttttct tggaactgag gtcagtatag
    25141 catcgctagc tgtaccgttc aatattatga atgaacagag gaaaaagacc catcatccgc
    25201 gtgtataagt cattaaaatg aacgatctag ctcacaaaca atcgcaagtc tataacaaaa
    25261 caaaaagcct tccttgaaag gaaagcttag cttctaattt taggtaaatg gttttttact
    25321 tcgaaataac ccctcttcat ttcgttaata taaggtgttt ttatcaagtg gataattaaa
    25381 actatatact agtttttcat tgttttgtgc attatgatcc tacgactttt tcagcatcaa
    25441 acgaagtttt aaattggcaa aaaacataaa aaaacagact atccctatca gatagtctgc
    25501 taaacgttga gttatcagcg tttttattat tccgcagcgt ttgcgtcttt gagaccgtat
    25561 tttttgttga aacggtccac acgtccgtct gcttgtgtga atttttgacg tccagtgtag
    25621 aatggatgtg agtcagaagt tacttcgaca cggatgactg ggtatgtatt accgtcttcc
    25681 cattcaactg tttcttgtga agtttttgtt gaacctgaca agaatttgaa accagttgtt
    25741 gaatccatga atactactgg gtggtaatct ggatggatgt tttctttcat gattttttcg
    25801 ctcctttgcc ctgctccatt tgctgaacca gagttgattt gtcgatttac aacattaaca
    25861 tcttaacatg tttcagtcaa ggttgcaatt atcttttcag atttctggtc ggattttttt
    25921 taccaaagga gacatcttga aattctttga aaaattgctg attgtttttt gttttacgca
    25981 aaaagcggat cagttgttcc gtatattcta atgaatctcc agtcatattt tttcgaagtt
    26041 tccaaatttc ttctaattct tcacttggca tcaataattc ttcttttcgg gtacctgatt
    26101 ttttgatatc gatggctggg aagatccgac gttcagataa ttcacgtgaa agttgtaatt
    26161 ctagatttcc agttcctttg aattcttcat agatcacgtc atccatacgg cttcctgtat
    26221 cgactaaagc ggtcgctaaa atcgtcaaac tgccaccttc ttcgatgttt cttgctgccc
    26281 cgaaaaattt cttaggttta tacaatgcgg ctgggtcaat cccgccactc aatgtacgtc
    26341 cgcttggtgg aacgactaag ttataggccc gagcaagtct agtaatacta tccatcaaga
    26401 taacgacatc tcgtttatct tcgactaaac gcattgcgcg ttccaatact aattcagaaa
    26461 ctcgagtatg gttttgcggc tgcagatcaa atgtagaaga taccacgtcg cctttcacgc
    26521 ttcgttctag atccgttact tcttccggtc gttcatcaat cagtagaacg attaattcta
    26581 cttccggata gttttcggtg ataccatttg cgatttcttt taatacagaa gttttccctg
    26641 cttttggcgg tgcgacgatc aatccacgct gaccaaaacc gattggagaa aagatatcga
    26701 tcattcgggt agatagtctt tgcggtgttg tttccagttg aagctgtctt tgcggataaa
    26761 gaggtgttaa ggctggaaaa tgcggacgtt cttttgcttc ttccggatct tttccattaa
    26821 cgctttccac gtgcatcaaa ccataataac gttccgattc tttaggtgga cgggcttttc
    26881 ctgccacttt gtccccattt ctcaaactga atcgtctgat ttgtgaggca gaaatataga
    26941 tatcttctgc acttggtcca taattgattg gccgtaaaaa accgtaacca tcttgaccaa
    27001 cgatatccaa tattccttcc atataataaa atccttgttt ctctgcttgt gcacgaatca
    27061 cagctagaga taattccttt ttattcatct tactgtaata aggaatttta aattctttgg
    27121 cataactata aacttctttt aacgttttgt tctctagttc tgccattgtc aaataatcgc
    27181 tcattcagct cccacttcct ctatcatttc aattgatgcc cctaaggctg ttagtttctc
    27241 gataatatga tcataaccgc gtaagatata atctacattg taaatcgtcg ttgtcccttc
    27301 tgccaatagt ccagcaatca ctaaacaagc acctgcccga agatcagaag ccacgacttc
    27361 tgtgccgtga agtttatttg gaccattcac gatgatcata tttccttcca cggtaatatc
    27421 agcgcccatt cgtgctagtt ccggcacatg gttcacacgt ttcgcataga tcgtatcgat
    27481 gatttctgaa gtgcctgtag tcatcaatag taacgcagta agtggctgct gcagatctgt
    27541 ggcaaatcct ggataaggat aggtcatgat gtttgcgggt tttaattctt tcgaggggta
    27601 aacttcgatt tcatcttcgc tgattttcat atggacacca atttcctgca attttgctat
    27661 aaagctttcc aaatgttcaa agatcacatt tttcactttg ataccatttc ctactgcagc
    27721 tgctaatgct agataggttc ctgcttcgat gcggtcagga atcatgaaat gccgacagcc
    27781 atgtaatgtt tcgactcctt cgatacggat cacatctgta cccgctcctc gaacttttgc
    27841 ccccatatta ttcagtaaag ttgccacatc tatgatttcc ggttctcgtg cagcgttttc
    27901 aattactgtc tgtccttttg cttttacagc agctaacatc acattgatcg ttgctccgac
    27961 tgaaaccacg tccatgaaaa tccgattgcc acgcaatcct ttattttctg tccgtaaata
    28021 catggcacca tgttcatttg tcacttcagc acctagagct tcaaatcctt tgacgtgtag
    28081 gtcaatcggt ctagggccta aataacagcc acctggcagg cctactaccg cttcgccaaa
    28141 tttaccaagc agtgttccca taaaataata agaagcacgc aaactattga tttttccgtg
    28201 cggcatcgga atcgatacga cttctcgagg atcgatttct agtatgttgt tggaaaaatg
    28261 gacagttgcg cccatgatct ctaaaatttc aattaatgaa tgaacatctt gtatgtccgg
    28321 cacaccttcc aagatcactg gggaatcagc taagattgca gctggaatca atgcgactgc
    28381 actatttttt gcaccgctga tcgttacttc accagctaaa ggtcggtttc ctttgattac
    28441 gatttttttc atctgtttcc accttgttta ttaatctttt ttcatggata tgcttgtatt
    28501 ttatcataga taaaaaaaag aaacgagcat ggtctgccgt tttaagtaaa aaagaatgga
    28561 gagcgacttc aaaagtcgtt ctccattcct atatatcgta tctgttatgt ggatgcttac
    28621 gctttgttag ctgaaccgaa ccagtcgata tgagatttag catcttcaac gattgctgct
    28681 ttacctggag ctaataattt acgagggtcg aatcctttac cttctttatc tttaccagct
    28741 tcgatatatt tacgagtagc tgcagcaaat gataattgga attcagtgtt aacatttact
    28801 tttgagatac ccattgagat agctttttcg atttgatctt gaggaatacc tgatccgcca
    28861 tgtaatacta aaggcatgtc tggacctaca gcatcagcaa ttgcttgtaa gtggtcaaaa
    28921 gctaatcctt tccagttttc tggatattga ccatggatgt taccgatacc gcaagctaag
    28981 tagtcgatac ctgtagcaac catttgtttg cattcttcaa tatcagctaa ttcgccgtta
    29041 ccgatgattc cgtcttcttc tccgccgatt gaaccaactt cacattcaac tgagatacct
    29101 tttgcatgtg ctttttcaac aacgtcttta gctaatttca agttttcttc aaatggtaga
    29161 tgtgaaccat cgaacattac tgaagtatag cctaattcga tacattccaa agcagcttcg
    29221 taatcaccat ggtctaagtg gatagcaact ggaacagtga tgttcattgc ttcaactaaa
    29281 tcagtgatta aatctttaca gattttgtaa ccacccatgt atttagctgc acccattgat
    29341 gtttggataa gtactggtgc ttgttttgct tcagccgctt caaggattgc ttgagtccat
    29401 tccaagttgt ttgtgttgaa tccgccgaca gcgtaaccgc ctttacgagc agcttttaaa
    29461 aattcagctc ctgatactac tggcataata aaattcctcc taagttttgt aaaactaaat
    29521 ttgttaagac aattcttaac caacgaccct attttaccaa acatttcggt agttttctac
    29581 taaaatcaat gaaaatgaaa attttcaaga agttttttgc aaaaaataaa atatccaata
    29641 gcgctttaat cgaaaaaagt cttttgcttt ctatcttttt tcctacaaat aataacaaat
    29701 cagaaaaaga gaaaagtgac aagaaaattg cttatcccct tctcttgcta cttttctctt
    29761 tctatcaagt tcaaaaatta ttttgtttcg tgatctgcta atgatgctcc gacaaatgct
    29821 ttgattaatt tttgcgggcg atttggacga gaaatcaatt ctgggtggaa ttggcatccc
    29881 acaaagaatt ttttctctgg cagctctacg atttctacta aacggttatc tggtgagaca
    29941 cctgaaaaga ctagcccttg ttcttcaaac aattgacgat atttgttatt gaactcatag
    30001 cggtgacggt gacgttcttg cacaacatcg gctccatcat atgcttcagc cgtcttcgtt
    30061 ccttttttga ttttacatgg gtataatcca aggcgtaaag ttccacccaa attctcgatg
    30121 ttttcctgat cagccattaa atcaataata ttgtttgctg tatctggatt cgtttcagct
    30181 gatgacgcat cttctaatcc aacgacgtta cgtgcaaatt cgacacaagc catctgcata
    30241 ccaagacaaa tacctaaaaa cggaacgtca ttttcgcgag cataacgaat cgcttcgatt
    30301 ttcccttcta ctccgcgatc accaaatcca ccaggaacca agattccatc tgcgtctttc
    30361 aaacgttctt ctacattttc tctcgttaat tcttgtgctt tgatccaatc gatctcgatg
    30421 tctgaattat aggcgaaacc agaatgtttc aatgcttcaa ctactgagat atatgcatct
    30481 ggtaactcga catatttacc gacaagcgca atccgtactt ttttcttcaa gttcaagaca
    30541 cgttcttcta attctttcca ttctgtcatg tctgcttctg gtgtatctag ttttaaatgg
    30601 tcgcaaacaa tttgatccat tccttgtgct tgtaaaacta atgggatgga gtaaagtgta
    30661 tctacatctg gcgactcgat aactgcttct ggttcgacat cacaaaattg tgccaatttg
    30721 tttttcacgc tttgagaaac aggttgctct gtacgtacca ccaaaatatt cggttggata
    30781 cctaaaccac gtagctcttt tacactgtgc tgcgttggtt tagttttcat ttctccagca
    30841 gctttcaagt aaggaatcaa tgttgtatgg atatacataa cattatctgt accgacatct
    30901 gctttcattt gacgaagtgc ttcaaggaat ggcaaagatt ctatatcccc tactgttccc
    30961 cctacttctg taatgatcac atctgaatca gtcattttag ctgctcgcat gattttttct
    31021 ttgatctcat ttgtaatatg aggaataact tgaacagttg ctcccaagta ttcgccctta
    31081 cgttctttac gcaaaacttc agaatagatt tttcctgtcg tcacgttgga gtatttgttc
    31141 aaattgatgt caataaaacg ttcgtagtgg cccagatcca aatctgtttc agcaccatca
    31201 tctgttacga atacttctcc gtgctgatat gggctcatcg tccccggatc cacgttgatg
    31261 tatggatcaa atttttggat agttactttt aagccgcggt ttttcaataa gcgtcctaaa
    31321 gaagctgcaa caatcccttt tccgattgat gaaacgacac cgccggtcac aaaaatatat
    31381 tttgtcataa gttcaaaaac tcctttttaa tttatgtgca ggaaaagtct cagacaataa
    31441 acaaagctcc ctattcttca ataagaatag ggagccgaaa tttctgtaaa cttctgaccc
    31501 tctaaaaggg tgcccaaggt gtataataca agctgactaa gggaaagtca agtactcaaa
    31561 tgaatactat ccgcttacaa ttttttcttt ttcgatatcc tttgagaaat tatttcttaa
    31621 gtcctttatg agtgcaaaaa aaatttaacc cacataataa gcttgatata aacatagcta
    31681 acgttaaacc taagcaaatc agaaaatcgt tactgaaaaa ttttaagtga aataatagat
    31741 aaaaaaataa aacaaacatt atgatagata atatcccacc taacaaagaa ctactttttc
    31801 ctacttgcag caacagttta tatagcttat cgtagaaaca atctttttct ttcaccctta
    31861 tttacctcct ttacgtaaaa aacgtagtcc acagttctcg gatttttggc atatcttttc
    31921 tattaaataa tatcaaaaac aaaacaattc cactaatttt ttcaaagatg accttttttt
    31981 caacaaaagt gccttgtagg gattcattga aggtaacgaa tcacataagt aaaaaataag
    32041 gagatgcgac aaaagtcgtg cctccttatt tctaaataaa cggtgttctc ctgccaactc
    32101 ctcggtcaca agtcacaata tccatcaaac atgagaagct gtttttgaaa ttatttcttg
    32161 tgattcggag ttaaatggta ggctcacaac ctctggataa acggtattca aaagccagtt
    32221 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct
    32281 taatactcga aactaaacgt ctttttcaaa acctcttggt aaacggtgtt caaaagcttg
    32341 catctgcggt aataagttca gccaattcaa aaacaagaaa agctgttttt gcttggctat
    32401 tcttattact tgttgcaggg cagctttttc acaacctctc cactaaacgg tgttcaatca
    32461 gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt ttccgttagt
    32521 ttctctaata ctcaggtcaa aacgctgatt tcacagcctc taattcacgg tattcctgtt
    32581 ttaataaagt tctagatatt gttctctttc ccactcggaa acagtttgtc tgaatgccgc
    32641 ccattcgaga cgttttgctt caacaaaatt tgagaagata tgttgtccta atgcgtcaat
    32701 cattacatta tccttacgca attctttgat agcattatgg atagttgaag gtaagtcctg
    32761 gatatgtgca gcttctcttt cttcctcatc cattacataa atgttacggt caacagctgc
    32821 tggcggtgtg atctgattac ggataccgtc tagtcctgct tgcaacaata ctgccattgc
    32881 caagtatggg tttgcagatg gatcaactga ccgcaattcc aaacgtgtgg ataaaccgcg
    32941 agattctggt acacgtacta atggcgaacg attacggcca ctccatgcga cataaacagg
    33001 cgcttcataa cctggaacga gacgtttata agaattgact gttggattgc aaacggctgt
    33061 gtaggcacga gcatgtttca gcaaaccacc taagaaatga taagctgttt cacttagttc
    33121 caattcaccg tctttatcat agaaaacatt tccttcatca ttgaacaaag acatattgca
    33181 atgcatacca gaaccattga tcccaaataa cggtttcggc atgaaagtcg cgtgaagtcc
    33241 atgcttgcgt gcaatcgttt tgacaacgag cttgaaggtt tggatattat cacaagcatc
    33301 taccacatct gcatatttaa agtcaatttc atgctgtcct ggcgctactt catgatgtga
    33361 agcttccact tcaaagccta gactttctaa ttccaacacg atgtcacggc ggcaattttc
    33421 acctaaatct gttggtgcaa aatcaaaata accaccttta tcattcaagg tcgttgtgat
    33481 cccaccgtct tcgtctaatt taaataagaa gaattctggt tcaggtccta aattgaaaga
    33541 ggtaaaacca aggtcgcgca tatctttcaa tgctcgtttc aaattcccgc gaggatcgcc
    33601 ggcaaatggt gtcccatccg gattatagat atcacaaatt aaacgagcaa cttttccatg
    33661 agcactttcc cacgggaaga tcatccaagt ggagagatct ggatacaaat acatatcgct
    33721 ttcttcaatt cgaacaaatc cttcaatcga agaaccgtca aacatcattt tattttctaa
    33781 tactttgtcg atctggctca ctggaacttc tacatttttg atcgtaccta agatgtctgt
    33841 gaacatcaat cgtaaaaatc gtacgttttc ttcctcaatg attcgtttga tttcttcacc
    33901 tgttatctgt ttctttacca tggtatccct cttccttttt taatttattc agtcaaagaa
    33961 ttgtctttgc aatccttgcg actgataatg acctgttttg cttaacccac cttgcgccaa
    34021 aatctcatca cgaaaaattt gtcgaacgtc atgatctgtc aacggcttct tgttttttgc
    34081 ttcttgtttt ttcgcctgtg ccatttcgta tacacgtttg atcccagcca tattcaatcc
    34141 ctctgaaaga tagtctttaa tctcaagcaa catatcaata tcattcagcg aatacatacg
    34201 tcggtttcct tcgcttcgtt ccggatgaac taaatcttgt tcttcataat agcgaatttg
    34261 ccgagcagac aaatcagtca gcttcataac tgtaccaatt ggaaatacgg acatagatcg
    34321 gcgcaattcc ttttctccca tcagacgtcc cctttcgttt tataagttgg ctaaagtgta
    34381 ccaattaaaa aaaatattgt caactgcata tgttaggttt tctaacatga gatctctatt
    34441 ttttataaaa taacaaaaaa ataagaacac aaaaaaggta tctagttgat tttcgtacca
    34501 gatacctttt attttttatt atactgtact atcctttatt agtgttttta tt
//
LOCUS       DAFCNS010000027        33817 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFCNS010000027 DAFCNS010000000
VERSION     DAFCNS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.027"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            299..1960
                     /locus_tag="LNE23_001192"
     CDS             299..1960
                     /locus_tag="LNE23_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6931061.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="LNE23_001193"
     CDS             complement(2030..2377)
                     /locus_tag="LNE23_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBK6931062.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="LNE23_001194"
     CDS             2461..3402
                     /locus_tag="LNE23_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931063.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="LNE23_001195"
     CDS             3403..4446
                     /locus_tag="LNE23_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931064.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="LNE23_001196"
     CDS             4452..5522
                     /locus_tag="LNE23_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931065.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="LNE23_001197"
     CDS             5512..6465
                     /locus_tag="LNE23_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6931066.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="LNE23_001198"
     CDS             6477..7487
                     /locus_tag="LNE23_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6931067.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="LNE23_001199"
     CDS             complement(7772..8593)
                     /locus_tag="LNE23_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK6931068.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="LNE23_001200"
     CDS             complement(8690..8968)
                     /locus_tag="LNE23_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931069.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="LNE23_001201"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="LNE23_001201"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBK6931070.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="LNE23_001202"
     CDS             complement(11724..12311)
                     /locus_tag="LNE23_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931071.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="LNE23_001203"
     CDS             12486..13808
                     /locus_tag="LNE23_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6931072.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="LNE23_001204"
     CDS             13963..14739
                     /locus_tag="LNE23_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK6931073.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="LNE23_001205"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="LNE23_001205"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBK6931074.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="LNE23_001206"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="LNE23_001206"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBK6931075.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="LNE23_001207"
     CDS             16629..17258
                     /locus_tag="LNE23_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931076.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="LNE23_001208"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="LNE23_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBK6931077.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="LNE23_001209"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="LNE23_001209"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBK6931078.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="LNE23_001210"
     CDS             19445..19981
                     /locus_tag="LNE23_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK6931079.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="LNE23_001211"
     CDS             complement(20021..21784)
                     /locus_tag="LNE23_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931080.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="LNE23_001212"
     CDS             complement(21787..23502)
                     /locus_tag="LNE23_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931081.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="LNE23_001213"
     CDS             complement(24281..24505)
                     /locus_tag="LNE23_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931082.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24696..27473
                     /locus_tag="LNE23_001214"
     CDS             24696..27473
                     /locus_tag="LNE23_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6931083.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27554..27973
                     /locus_tag="LNE23_001215"
     CDS             27554..27973
                     /locus_tag="LNE23_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBK6931084.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="LNE23_001216"
     CDS             27985..28455
                     /locus_tag="LNE23_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6931085.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="LNE23_001217"
     CDS             28472..29251
                     /locus_tag="LNE23_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6931086.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="LNE23_001218"
     CDS             29238..30059
                     /locus_tag="LNE23_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6931087.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="LNE23_001219"
     CDS             30076..31125
                     /locus_tag="LNE23_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6931088.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="LNE23_001220"
     CDS             31138..32142
                     /locus_tag="LNE23_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6931089.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="LNE23_001221"
     CDS             32775..33794
                     /locus_tag="LNE23_001221"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK6931090.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11042 a         5991 c         6908 g         9876 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgt
//
LOCUS       DAFCNS010000028        33262 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFCNS010000028 DAFCNS010000000
VERSION     DAFCNS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.028"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(40..228)
                     /locus_tag="LNE23_001222"
     CDS             complement(40..228)
                     /locus_tag="LNE23_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBK6931091.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            498..860
                     /locus_tag="LNE23_001223"
     CDS             498..860
                     /locus_tag="LNE23_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6931092.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            912..2594
                     /locus_tag="LNE23_001224"
     CDS             912..2594
                     /locus_tag="LNE23_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBK6931093.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2711..4747
                     /gene="recG"
                     /locus_tag="LNE23_001225"
     CDS             2711..4747
                     /gene="recG"
                     /locus_tag="LNE23_001225"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBK6931094.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4796..5797
                     /gene="plsX"
                     /locus_tag="LNE23_001226"
     CDS             4796..5797
                     /gene="plsX"
                     /locus_tag="LNE23_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBK6931095.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5919..6161
                     /gene="acpP"
                     /locus_tag="LNE23_001227"
     CDS             5919..6161
                     /gene="acpP"
                     /locus_tag="LNE23_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK6931096.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6429..7433
                     /locus_tag="LNE23_001228"
     CDS             6429..7433
                     /locus_tag="LNE23_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931097.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7434..8378
                     /locus_tag="LNE23_001229"
     CDS             7434..8378
                     /locus_tag="LNE23_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931098.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8378..9340
                     /locus_tag="LNE23_001230"
     CDS             8378..9340
                     /locus_tag="LNE23_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931099.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9367..10281
                     /locus_tag="LNE23_001231"
     CDS             9367..10281
                     /locus_tag="LNE23_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931100.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10307..12088
                     /locus_tag="LNE23_001232"
     CDS             10307..12088
                     /locus_tag="LNE23_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6931101.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12436..13122
                     /locus_tag="LNE23_001233"
     CDS             12436..13122
                     /locus_tag="LNE23_001233"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBK6931102.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13142..16723
                     /gene="smc"
                     /locus_tag="LNE23_001234"
     CDS             13142..16723
                     /gene="smc"
                     /locus_tag="LNE23_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBK6931103.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16732..17541
                     /locus_tag="LNE23_001235"
     CDS             16732..17541
                     /locus_tag="LNE23_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6931104.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17552..18550
                     /gene="ftsY"
                     /locus_tag="LNE23_001236"
     CDS             17552..18550
                     /gene="ftsY"
                     /locus_tag="LNE23_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBK6931105.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18737..21004
                     /gene="gshAB"
                     /locus_tag="LNE23_001237"
     CDS             18737..21004
                     /gene="gshAB"
                     /locus_tag="LNE23_001237"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBK6931106.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21093..21545)
                     /locus_tag="LNE23_001238"
     CDS             complement(21093..21545)
                     /locus_tag="LNE23_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBK6931107.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21695..22645
                     /locus_tag="LNE23_001239"
     CDS             21695..22645
                     /locus_tag="LNE23_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK6931108.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22638..23597
                     /locus_tag="LNE23_001240"
     CDS             22638..23597
                     /locus_tag="LNE23_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK6931109.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23594..24349
                     /locus_tag="LNE23_001241"
     CDS             23594..24349
                     /locus_tag="LNE23_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6931110.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24372..25325
                     /locus_tag="LNE23_001242"
     CDS             24372..25325
                     /locus_tag="LNE23_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6931111.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25969..26265)
                     /locus_tag="LNE23_001243"
     CDS             complement(25969..26265)
                     /locus_tag="LNE23_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBK6931112.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26620..26976
                     /locus_tag="LNE23_001244"
     CDS             26620..26976
                     /locus_tag="LNE23_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK6931113.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26986..27885
                     /locus_tag="LNE23_001245"
     CDS             26986..27885
                     /locus_tag="LNE23_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBK6931114.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28402..29133
                     /locus_tag="LNE23_001246"
     CDS             28402..29133
                     /locus_tag="LNE23_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK6931115.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29278..31305
                     /locus_tag="LNE23_001247"
     CDS             29278..31305
                     /locus_tag="LNE23_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBK6931116.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31413..32510
                     /locus_tag="LNE23_001248"
     CDS             31413..32510
                     /locus_tag="LNE23_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBK6931117.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32817..>33262
                     /locus_tag="LNE23_001249"
     CDS             32817..>33262
                     /locus_tag="LNE23_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6931118.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        11137 a         5762 c         7123 g         9240 t
ORIGIN      
        1 aaaaccgttc actatctgaa cggtccaagt atcattcaat taaacgcgtt caacttttcc
       61 tgatttcaaa gcacgagttg acacccaaac ttttttaggt tttccgtcga ccataacgcg
      121 aactttttgt aagttaggtt taacagtacg ttttgttgag ttcatagcgt gtgaacggtt
      181 gtttccactt tttgtcttgc gacctgtaaa gtaacaaact tttgccatgg taatttcctc
      241 ctttgccttg atttggagca atagaataat tcagctcata ccagactaac atatcatata
      301 gaatggaaat ttgcaagtac attttcatag atacaattga caattgagaa gaataattgt
      361 aaattaaaca aggttatacc tgaaattttg caaggttttt gagtactttg ggtaactgcc
      421 ttttatttca agttgcgcta tgataaaata tgatagaaga aaaatgggag ttttttaccc
      481 gttaaggagg ctcattcatg gctgtaaaaa ttcaaacgtc agctggaacc attgaaatta
      541 ccaatgatgt aattgcaaca gttgttggtg gcgccgcgac agatgtgttc ggtgttgtag
      601 gaatggccag caaaaatcaa attaaagata atattactga gattttgcgt aaagaaaatt
      661 actcaagagg agtagttgtc cgccaagaag ataatggtat tgctgtagat gtttatacga
      721 ttatcagcta tggtacgaaa atctctgaag tttcccgcaa cgtccaagaa aaagtgaaat
      781 acaatctaga ggcattatta ggcgtaactg ccaattcagt taatgtcttt gtacaaggtg
      841 ttcgtgtact gccagactag gcaggagact ggctggtaac tcagcagtcg atctgaagga
      901 ggattatcta ggtgagtgta acagaaataa gtgcaagcca gttccaggaa atggtccagg
      961 caggtgcgaa tcgtctgcaa gtaaatgcag aatacgtcaa ttcattaaat gtattccctg
     1021 tgccagatgg cgatacggga acaaatatga atctatctat gactagtgga gctaaggcag
     1081 tagtagattc aacctcagaa aaagtaggag aattagcagc aatgctttca aaaggattgc
     1141 tgatgggtgc gcgcggaaat tccggcgtta ttttgtctca attgtttcgt ggattttcta
     1201 agcaaattcc agatgttgtc tcattaaatg cttccgatct tgctgcagcc ttcactcatg
     1261 gagttgaaac tgcgtataaa gcagtaatga aaccagtaga aggaacgatt ttgacagtag
     1321 ctagagaagc agcgaaagct ggagaaaaga aagcaaaaag tactgatgat gtcgttgaag
     1381 taatgacagc tgttgtaaaa ggggcaaagc gtgctttagc taaaactcca gacttgcttc
     1441 cagtcctaaa agaagtcggt gtcgtagaca gtggagggca aggactgctg tttgtttatg
     1501 agggcttttt aagtgcattg aatggagaat tccaagcgga tgaaacatat gaaccttctc
     1561 cagcagaaat ggatgatatg gtcaatgcag aacatcatcg cagtgtccaa ggtcaaatgg
     1621 cgacagaaga tatccatttt ggttactgta cagaaatcat ggtaaaaatc ggagaaggac
     1681 ctacagtcga tagtacattc gattacgaag aattccgtaa ctatttagat ggtataggag
     1741 attctttact tgtggtcaat gatgacgaga tcatcaaagt ccacgtgcat acagaaaacc
     1801 caggagaagt cttgaattat ggacaaaaat tcggttcatt agtcaaaatc aaagtcgaca
     1861 acatgcgctt gcagcatgag acgattttgg aacacgatga agaagtgaac gagtttgcga
     1921 aaaaagaaac accacgtatc ccgtatggta tcgtagcgat cgctgctggt aaaggcgtcc
     1981 aagagctgtt tgaaagttta ggcgctaatt atgttatcag tggtgggcaa acaatgaatc
     2041 caagtacgga agacattgtt aaagcgattc aagaagtaaa cgcagataaa gtgattgtct
     2101 tgccaaacaa taagaacatc ttcatggcag ctgatcaagc agcagaagta gcagatatcc
     2161 cagtagcagt tgttccttca agaacgattt cccaaggatt gacagctatg ctttcattca
     2221 atgatcaagc ttctttagaa gaaaacaaag aaacgatgac agaaatgttg tctagcgttg
     2281 tgagcggtca agtcactcac gcgatcagag atacctcaat tgacggtgta gagatcaaag
     2341 aaggagactt tttgggcatg atcgatggaa aaatcctgat ctctaaccca gatatcacag
     2401 caacagcgtt aagtacacta gaacaaatga tcaatgagga cacagagatc gtaacgatct
     2461 tggcaggtga tgaaggaaca gttgaacaag caaatgaatt tgctgagaaa ctaacagaaa
     2521 aacatcctga tttggaaatc gagatccacc aaggagatca gcctgtctat ccttatctat
     2581 tttctgctga ataatttctg tgtcagaata tctttctaac acagtattca ttcataaaag
     2641 gccttcggtt agtgatcagg tatgatcgct gaccaagtcc tttttttgtt aagggaggga
     2701 ggaaaaattt atgcgttctt tagatgatcc aatcactatg ctggcaggag tcggagaaaa
     2761 aagagcagct agtcttgcca gcttgggcat ccaaacgatt gaagacttac tgacttatta
     2821 tcctttccgc tacgaagata ttcaggaaag aaatctaaac gagatccaag atcaagaaaa
     2881 ggttactttg aaaggaatcg tagtctcgcc tcctgtgatg aatcgatttg gttataaaaa
     2941 aagccgattg caatttcgta tgatgcaaga acatgatgtg ttcaacgttt ctttttttaa
     3001 tcaaccttat ctaaaagaaa aagtcatttt atcggaggaa atcgccgtat acggtaaatg
     3061 ggatgcaaag cgtaaagcat tgaacgggat gaagatttta ggttctcaaa caatggatga
     3121 cttttcgccg atatatcacg tcaacaaatc aattcgtcaa acgacattag ttgatctaat
     3181 caaaaaaggg tttcaagaat acggcacatt gattgaagaa aatctgccaa acaaccttgt
     3241 ggagaaatat cgactgcttg accgagcttc tgctgtaaga agtatgcatt ttccaaaaga
     3301 ccacgaagaa aaccatcaag ccaaacgacg tgtcgttttt gaagagttct ttttatttca
     3361 aatgcgtctt caaggattga aaaaagcaga aaaagcagaa acgaatggtt tggagatcct
     3421 ttatgatgtc caaaaattga aagaatttac tcaaaagcta ccttttgaac tgacaaatgc
     3481 acaaaaacgt gtaacgaatg aaatctgtcg ggatcttcgc agtccgcagc atatgcagcg
     3541 acttttgcag ggagatgtag gtagcggaaa aacagtggtt gccgcgattg cgttatacgc
     3601 taccgttaca gcaggtttcc aaggtgcgtt gatggtgcct acagaaatct tggcacaaca
     3661 acatatggaa agcttgcaac agctgtttga tcccaatgaa gtaaccatcg cattgcttac
     3721 ggggtcaacg aaagcaaaag aacgtcgtga gctccttgaa aaactagagc aaggggaaat
     3781 taacatcgtg atcggcacac atgcattgat ccaagatgga gtcgaatttc agaatttagg
     3841 tctagttatt acggatgagc agcaccgttt tggcgtgaac cagcggaaag tacttcgtga
     3901 aaaaggctgg cgaccagatg tcttatttat gacagcgact ccgattccta gaacattagc
     3961 tattacagca tatggtgaga tggatgtatc tatcattgat gaacttccag ctggacgtat
     4021 cccgatcgaa acacgctggg taagaacacc tcaattggac agtgtactgg attggacgaa
     4081 aaaagaactg gcaagaggac atcaaatgta tgtcatctgt ccgctgattg aagaatcaga
     4141 agcattagat gtaaaaaatg cagtggaaat ctatgaaaaa cttcgtgaac tatttgcacc
     4201 aaaatatgaa gttggcttgt tacatggtaa aatgaagaat caggaaaaag aagtaatcat
     4261 ggaagccttc aaagacaacc agatgcagat attagtttcg actactgtaa ttgaagtagg
     4321 ggtaaacgtg ccgaacgcga ccgtcatgct gatcatggat gcggatcgat ttggattggc
     4381 acagctgcat caacttcgtg gacgtgttgg acgcggaagt gatgcttcct actgtatctt
     4441 agttgctaat cctaaaaatg aactaggtgt ggaacggatg aaaatcatga cagagaccac
     4501 caatggtttt gtactaagcg aaaaagatct ggaattacgt ggtcctggtg aagtatttgg
     4561 cttccgtcaa tctggtctgc ctcaatttgc gattgctgac ttggtgacgg atggaaatgt
     4621 tttggaggta gccagagaag aggctgcagc cctttggcag ataaaggatt ggcagatgct
     4681 tcctgagtat cagcctttag cgcaaagcct ccaaatagaa aatcaggata atcgttattt
     4741 cgattagcag actgtgctat actttatact gatgacggaa aaaggagtgg aaacaatgaa
     4801 aattgccgta gatgcaatgg gcggagatca cgcacctcaa gcaattgtag aaggcgtggc
     4861 tttagcgcag caagattttc cagaagtaga attcttactt tacggaaaag aagcagaaat
     4921 caagaaatac ttaacaaatg aaaaaaacat cacgattatc catacagacg aaaaaatcaa
     4981 tagtgatgat gagccagtaa aagcaattcg acgaaaaaag actgcatcaa tggtccttgc
     5041 tgcacaagca gtgaaaaacg gtgaagcaga tgctgtattt tcagcaggga atacaggcgc
     5101 gctactagca gcagggttgt tcatcgtagg acgtatcaaa aatatcgaac gccctggctt
     5161 gatgtctacc ttgccagtag tagggaaaga aggtgccggt ttcgatatgc tggatcttgg
     5221 cgctaacgca gaaaacaaac cagaacattt gttgcaatac ggtattttag gttcttttta
     5281 tgcacgtaaa gtccgaggta tcggacgtcc tcgtgtcgca ctcttaaaca acggaacaga
     5341 agcaacaaaa ggtagtgaag tgacgaaaaa agcctatgaa ctccttcaga atgaaacaag
     5401 cttgaatttt attggaaacg tagaagctcg tgagctactc aatggtgtgg ctgatgtagt
     5461 ggtcacagat ggatttacag gcaacgctgt tttgaaatct attgaaggaa cagcaatgaa
     5521 tatgatgaat ctattaaaat ctgctattct aaatgaaggg atcaaaggaa aaatgggcgc
     5581 tatgctttta aaagacggct tacgttcttt gaaagcagaa atggattatt ccaaacatgg
     5641 cggtgccgtt ttgtttggct tgaaagcacc agtcattaaa actcatggag caacaggccc
     5701 cgatgctgtt cgctatacga tccgccaaat ccacacaatg cttgagacag atgtagttgg
     5761 acaattagtc gaacaatttg aaaaaagcga agaatagtat ttggatatct gaagcaatcc
     5821 gttccattga aaaaaaggtg gacattcctg aaaaatgcca gtaaaattag ccatggtgta
     5881 ggtaattgac aagtgtatga ttcttggagg tgaaaaagtt gactcgcgaa gaagtattta
     5941 acaaagtagc gaaaattatt tcaaatcatt ttgagataga taccgataaa gtgacggatg
     6001 aattgaacat caaagatgat ttaaaagctg attcaattag tattatggaa ttcgttttag
     6061 agcttgaaga tgaattcggt acggaaattt cagatgaaga cgctgaacaa attgaaacag
     6121 tcggtggcgc agtagattat atcagcagcc atcttaaata atttcaaata aaagcaaaga
     6181 aggataaaaa gctgcataca gctttttatc cttctttttt taatcttttt gacacgtaaa
     6241 agtcattttt ctgataattc tggcgataaa cagaaattat gttgcaaagt aaagcgattt
     6301 tctatataat tattaataat tcattacaca aagataagaa taacctttgt tataaaacgc
     6361 tttattctgt gtaatgtcct tttttattaa gaaaaacttc catttaggaa gcagagaggg
     6421 gtattcgatt gtccgataac caattattag aagttcagaa cctacataca ggattccgac
     6481 tcaaagatga ctattacgat gcagtagatg acgtttcatt tactttagac aaaaacgaga
     6541 ttttagcaat cgttggggaa tctgggtgcg ggaaaagtac cttggctact acgattatgg
     6601 gattacttga tcctaataac acaaaaatta caggtgagat tttatataac gatctgaatt
     6661 taatcgacct gaacgaaacg ctctataata aaatccgcgg aaatgatatc ggaatgattt
     6721 tccaagatcc gttgtctgca ttaaatcctc tcatgagaat cgaagaccag atcaaagaag
     6781 gactttccta tcacacaaag atgaatgcag aacagcgtca agctcgtgca ctagaattgc
     6841 tagaacaagt agggattcct aatcctgcga gagttggacg ccaatatccc catgaactat
     6901 caggcggtat gagacaacgt gtcatcatcg caatcgcaat cgcctgcaaa cctccaatcg
     6961 tcattgcaga tgaaccgacg acagccctag atgtaaccat ccaagcacag attcttgatt
     7021 tactgaaaga ccttcaggaa gaaacaaaaa cagggatcat cttgatcact catgacttag
     7081 gagtcgtagc agaaatggct gatcgagtgg cagtgatgta tggcggacag ttcgttgaag
     7141 tagcaggtgt aaaagaactg tttgatcatc ctaagcatcc ttatacacgc tctttactga
     7201 actctatacc acaagaagga aatcatgatg cagaactcca tgtgatcgaa ggtgtcgttc
     7261 cttctctgaa aaacatgcct cgtaaaggtt gtcggtttgc accacgtatt ccatggatac
     7321 cagcaagcga tcatgaagaa gagcctatcc ttcatgaagt cgcaccgaac cacttggtac
     7381 gttgctcctg ctataagcat ttccacttta gagacgaaaa aggagaggtg tagatgacag
     7441 aattaatcac aatcaaagac ctgaaagttc attaccctat tcgcagtgga ttcttcaatc
     7501 gagtaacgga tcatgtatat gctgtagatg gtgtggattt tattatcgaa aaaggaaaga
     7561 catatggttt agtgggagag tctggctctg gaaaatctac tacgggaaaa gccgttgtgg
     7621 gattagaaaa ggtaacagcc ggacaaatca tgtatgaagg aaaagatgta acgaagcgaa
     7681 gcaaccgcaa aaaaatgggt tataacaagg atgtgcagat gatcttccaa gattcaatgt
     7741 ccagtttgaa tcctaaaaag cgagtgttgg atatcattgc tgaacccatc cgaaattttg
     7801 aacggttaag cgatcaagaa gaaaagaaaa aggtaaaagg actgctagat atcgttggga
     7861 tgccggaaga tgcgctatat aaatatcctc atgaattttc aggcggacaa cgtcaacgtc
     7921 taggagtagc ccgtgctgtt gctaccaatc caaaactgat cgtagccgat gaacctgtat
     7981 ctgctctgga tctatctgtt caagcgcaag tattgaattt catgaaacga atccagcaag
     8041 aatacggcct cagctattta ttcatttcac atgatttagg tgtagtaaaa cacatgtgcg
     8101 acaatatcgc aattatgtac aaaggtcgat ttgtagaaat cggtacaaga gaagacattt
     8161 ataatgaccc gcgacatatc tatacgaaac gtctgctttc agctattccg caaatcgatg
     8221 tagaaaatag ggaaagccac aaagaaaatc gacgaagagt agaacaagag tatatccaaa
     8281 accaaaaaga atactatgat gcgactggtc gagtctacga tttgcgtacg atcacaacta
     8341 cacacaaagt ggcgttgaaa gatggaggtg caagctaatg tggaaaacga ttctgcgacg
     8401 tgttttattg atgatcccgc aagttattat tttaagtgtt ctgatcttct tattagccca
     8461 agcgatgccg ggggatccat ttaccggttt gatcaatcca aatcaggatc cggcagtgat
     8521 cgaacagatg cgacaagcag ctggcttgaa tgacccatgg tatgtccagt acttccgttg
     8581 gatcggaaat gctttgcaag gcgattttgg tcaaagtttc ttatacaaac gctctgttgc
     8641 tgacttgatc ggcgaacgga tcatcaacac tttatatcta tcgatcttga cagttgtcct
     8701 tacttatttg attgctgtgc ctctggggat gcttgctggc cggtatcaaa attcaatctt
     8761 ggataagatt gtcgtaatct acaacttctt tagttttgct gttcctttat ttatcttcgg
     8821 cttgatcatg ctatttgttt ttggttatcg tttaggctgg ttcccaacaa gtggatcaca
     8881 aacactaggt ttgcaaggtg gatttatcac tcaatggata gaccgtttga aatatattct
     8941 gttaccatca attacccaag catttttggc gactgccgtc acaatccaat atctgcgaag
     9001 tgaagtaatc gattcgaaat cgctcgattt tgtcagaaca gcccgttcca aaggggtacc
     9061 gacgaataaa gtattcagtc gacatatttt ccgaaatgca gcgttgccga tggcttctca
     9121 aatggggtat gaaatcactt cgttgattgc tggttctgta gttatcgaaa agatttttgg
     9181 ttatccggga gtcggaaaac tatttattga ttccatcggc caacgggact ataccgtgat
     9241 cacatcctta gtattgattt taggaatcgc gacgctagtg gggacattac tttcagatat
     9301 catcatgagt atcgttgacc cgcgtattcg gattcaataa aaccaatcag aaaggagaat
     9361 ggatcgatga gtgaaaaaaa caatacagca gttcaagaaa gtattcctcc tatggggata
     9421 cgaatgattg ctcgagaatt taaaaaagat aaaattgcta ttttttcatt aggtttgcta
     9481 gtggttttat tattagtgat ctttatcggt gcgatgctga ccgatcaaga caaagtgatg
     9541 tatgttagta tcttcgacaa atacgctgca ccaggaacag tttcgacaca aggacaaaag
     9601 ttcattctgg gagcagatga aggcggacgt gatgtattag gacaattgat cataggtgct
     9661 agaaactctg ttacgatcgg atttgcgatc acgatcctta cttcgatcat cggggttgga
     9721 ttagggatca tctctggcta ctacggaggg atcattgaca acatcttgat gcggatcgtt
     9781 gattttatca tgattctacc gatcatgctg atcattatcg tcttcgtatc gattatttcc
     9841 agatacaatg tctggtcctt tatattcatc atgagtgcat tttattgggt agctaaagcc
     9901 cgattattcc gaagcaagac attatctgaa gcaagaagag attatgtcag tgcctcaaaa
     9961 acaatgggaa ccaatgattt taaaatcatg ttccgcgaaa tcatgccgaa tctaagttca
    10021 ttaatcatta ctaatttgac gatgaacttt gcggcaaaca tcggtatcga gacaactttg
    10081 actttcttag gtttcggctt gccggcaaat gtacctagtt taggtacatt gatcggttat
    10141 gcaagtaacg gagaagtctt agtgaatcgg caatggatct ggttacccgc atcaatcctt
    10201 attttagttt tgatgttgag tatcaattat gttggacaag cattcaagcg ttctgcagat
    10261 gcgcgccaac ggttaggcta gtatagaagc gaagggggaa ataggaatga agaaaacgat
    10321 ttttggattt gcagcgctga tgtctgctgt cgttcttgca gcatgcgggg gcggaggtac
    10381 agatacctct ggaaacaact ctggaggcaa cggcaataac aaagaggaat taacttttcc
    10441 aattgcaaca aaaaataatg atgatgcaat cgaagacggc gaattagaag ctgcggtcgc
    10501 aacggacagt cagtttaaag gattgttcca gtgggagttt taccaagatt catatgatgc
    10561 agcttttatg gctccttctc atgaaccgct atttgcaaac gatgacgatt tcagaatcac
    10621 agacgacgga gctgcaacgc ttgatctaga tgaagatacg aaaaaagcga caatcaaagt
    10681 aaaagacaac gtcaaatggt cagatggtga agatgtaacg gcggacgatg tgattttccc
    10741 ttacgaaatc attgggaata aagattacac tggtatccgt tatgacgaca ctttccgtaa
    10801 catcgtaggt atggaagaat atcatgatgg aagtgccgac acaatctccg gaatcaaaaa
    10861 aatagacgac aaaacagttg aaatcgaata taaagaagta aacccaagca tgcttcaatc
    10921 gggtggtggt atctggtcat atgcagcacc aaaacatacg ttagaaggtg tagcaatcaa
    10981 agatatggaa tcaagcgatc aagtacgtaa aaatccagtg acttttggcc cttattacat
    11041 gagtaatatc gttgcaggag aatcagtaga atacttgcca aacgagcact actggaatgg
    11101 tacgcctaaa ttgaaaaaga tcacgatgaa atctgtaccg atggcaagtg caactgaagc
    11161 attgaactct aaattatatg atatgatttt ccaaatgcca acagatactt acgataccta
    11221 taaagaaatt gctggctata caaacttagg ccgtgaacaa acttcttata cttatctagg
    11281 attcaagtta ggtaaatgga atgcagatac aaagagtgtg gaatacaatc cagattcaaa
    11341 aatggctgat aaatcattac gtcaagcaat gggctatgca ttagataata atgcagtagg
    11401 tgaacgtttc tatgcaggtt tgagaagcaa tgcgacttca ttgattcctc cagtattcca
    11461 gtcataccat gatgatgaga tgaaaggatt cacacaagat ctagacaaag cgaaaaaact
    11521 attagatgat gctggctaca aagatgtgga tgacgatggt ttaagagaag acaaagatgg
    11581 aaataaacta acaatcaact ttgcttcgat gtccggtggg gaaacagcgc aaccattggc
    11641 agattactat atccaacaat ggaaagaaat tggtttggat gtccaataca caacaggtcg
    11701 tctgatcgaa ttcaattctt tctatgataa attagaaaac gatgatcctg aaattgatgt
    11761 ttaccaagct gcttggaata caggaactga tccaaatcca agtggactat ggggaccaaa
    11821 tgctgcgttc aactacactc gttatgaatc agaagaaaat acaaaattga tcaacgatat
    11881 cagttcaagc aaagcatttg atgaagagta ccaaaaagaa gcatatcaaa aatggcaaca
    11941 atatgcattt gaagaagcat ttgcgattcc aacgttgttc cgtaatgaag tattgccagt
    12001 caacgaccgt gtcaaagact gggattggtc ttatgatgta ccaaacccat gggcaaatgt
    12061 ttctgttaca gcagattcac gttcataatc actaaaaatg accgaaaaag ggcgatgtcc
    12121 tttttcggtt ttttgatatt tttctgttta tttctctaat attcaggtca aggcgctgat
    12181 ttcatgactt ctccaataaa cggtgttcaa agtccagttt ctcggtatta agtcaaattt
    12241 atgcaaaaat atggaaagcc attttcacaa aattttttct tcatactcga aactaaacga
    12301 atttttcaca acttctcgtc aatcactagg attattaaag ctttacttac cttttgaccg
    12361 aaccatttca aatttgacaa aaaaccagta taataaggaa gtatcgaaaa aatatccata
    12421 aaaggaatgt ggaaaatgga caaccagctg acaaaagaac taaaagaaaa ctataacatc
    12481 gtttttcacg atctaaattt gcttgaacaa gcttttaccc attcatccta tgtgaatgag
    12541 catcgcaatc tgcaattatc ggataatgag cgacttgaat ttttaggaga tgccgtttta
    12601 gaactgatgg tttctcagta tttattccgt ttatatcctc atttgccaga aggaaaattg
    12661 acaaaaacac gagctgcgat cgttcgcgaa gacagcttgt cgaaatttgc caaagaatgt
    12721 cactttgatc aatatgtttt gttgggcaag ggagaagaga attcaggagg acgtacgcga
    12781 ccagcattgc tttgcgacct attcgaagct tttttaggcg cgttatatct tgaccaggga
    12841 ttcgacacga cccatgcatt catcgaaaaa gtgatttttc ctaaagtcaa agcaggtgct
    12901 ttttcacatg agatggatca taaaacaaaa ctacaagaag tcttgcaaaa atcaggtgat
    12961 gtatcaatcg aataccgatt gatcaatgaa gaaggtccag ctcatgaacg agtattttgg
    13021 atcgaagttt atgtagatga tcaattgatt ggcactggac aagggaaatc gaaaaaatta
    13081 gcagaacaag ctgcggcgga aaatgcattg gcagcactgt aaaagtaagt gagggaaagc
    13141 tttgtatttg aaacgaattg aaatagccgg ttttaaatca tttgcagaca agacagtcat
    13201 tgattttgaa aatagcgtca cagcagtggt tggaccaaat ggcagcggaa aaagcaatat
    13261 tacagaggct atccgctggg tattaggaga acaatctgct aaaagtctgc gaggcggaaa
    13321 aatgccggat atcatctttg ccggttccga tacccgtaag cagctgaata ttgcagaagt
    13381 cacagtgatt ttagacaata gtgatcatta tctgccattg gagtacaatg agatcagtgt
    13441 aacccgccgt tatcgacgga ctggggaaag cgagtttttc atcaataaac aaagctgcag
    13501 attaaaagat atccaagaat tgttcttaga ttctggatta ggaaaagaat cgttctcaat
    13561 catttctcaa ggaaaagtag aagcgatttt cagtagtaaa ccggaagacc gtcgtgggat
    13621 ctttgaagaa gcagctggtg tcttgaaata caagcagcgg aaaaagaaag cagaacagaa
    13681 gctttttgaa acagaagaca acctcagccg cgtccaagac atcatccacg aattggaaga
    13741 acagttaact cctttggccg cccagagtga ggcagcaaag gagtttttac ggctaaaaga
    13801 aacactgact caaaccgatg tctctttgat ggttgctgaa atcaagacag caaaaaaaga
    13861 ttgggacaac aaacaagcac agcttgcgaa attcaatcta gaattaggca aactatctga
    13921 gtcgatccaa gaacaagaat ccattttggc gaagcagcgt aaagaaaatg cgcaagccga
    13981 tcgtctgatc gaaaaaaatc agcaagtatt attagatctt tcggaaaaac tgaagcagac
    14041 agaaggacaa aaagatgttt tgcaggagcg gacaaagcat acacaaaaaa gctcgcaaga
    14101 atatcaaact tcattagcag aagcacaaaa aaaggttaaa cattttgaaa agcttcaaga
    14161 aagcttgatg aaagcagcag cagaaaaaga aacagagatc caaaaagctg aagcaaatct
    14221 cataaaaaca cagcaagaat tagaaaaata ccaaaaatca acgaaagaat tattggcaga
    14281 attaagagat caatatgttg atttgatgca agaacaagca gctgtaggaa acgaactgaa
    14341 gtatctagaa cgtcaatata tacaagaaac agcaaaaagc aagcagacgt tagccaagca
    14401 atcggaagta gaagcatcag tagaccgatt gatcttacaa aaagaagaat tgacacaaaa
    14461 gcaggcgcag ctaaaatcat ctttaactga aacaaaagag aagttagaaa tgatccaaca
    14521 aaacgggaaa aagttccaag aagcattagc aaaagaacag cctaaaatgt atcaattaat
    14581 gaatcaagtc cagcagctac gtgctcgtaa gaaaagcctt caagaaatcc aagaaaacta
    14641 ttttggtttt taccaagggg ttcgattggt cttgcagcat aaacagcagc tttcgggtat
    14701 tgtcggagca gtggctgaac tgatcgatgt tcctgcagat ttcacgttag ctatcgaagc
    14761 agctttaggt ggggcagccc agcatgtcat cgttgaaaat gaaaaagatg ctcggcaagc
    14821 catcacgtat ttgaaacagc aaagaggagg aagagcaaca ttccttccac tgacgacaat
    14881 caaaccaaga caattaccgg ctcatatatt gacacaagca gcagctgtag aagggtttat
    14941 agggattgcc agtgaacaag tcagttatcc cgatcaaatc caaactgtgg ttcaaaacct
    15001 gctaggaacg atccttttag ccaaagactt aactagtgcc aatgcaatcg cgcaaacgat
    15061 ccgctatcaa tatcgagtgg tttccttaga aggtgatgtg atgaatgccg gaggatccat
    15121 gacaggtgga gcgaataaac gaggaaacca aggcagtttg tttgtacaaa atcaagaatt
    15181 gaaacagttg actagtgaat ttgaagaagc agataaacaa ctccaagcac aagaaaaaaa
    15241 agttcaggag ctgcaacaag aaacagctcg tttagccgaa gaacaagaag ttttgcgtac
    15301 acgaggcgaa caattacgtt ttgaagaaca agaagcgaca aatcagctac aaaacatcat
    15361 aaatgaactt gaacgttttg aaaaagaaaa gcaaatctca acttttgaga cacgtgaact
    15421 gcagcagttt atcgaagatt acgaaaagca aacgaacgaa ttgaaagaca aacaaacaga
    15481 tcttgaatcg caacgacaaa aaatcgacga agaaatcaaa tcgttaagtc aagaaagcga
    15541 ccagatggaa gccagaagag cacaagtaca aagtcaaaaa gcacaagaac aagcagatct
    15601 tgctgtcttg aaagagcagt ttaatcatct gcagatccag ctccgcggtg cccgagtcca
    15661 aaaagcagag gcaaccgaac gtcaagaagc gatagaaaag cagttggcga cattgacagc
    15721 agacttttct gatcatgaag tcacagaaga aagtttggaa aaacaaatta acgaattatc
    15781 tgctcagcga gaaacattaa aagccgagtt ggcaaaagcc aaagagcagc gagatagaag
    15841 gcaaaaggag atcgataagc ttgaagcagt cttggcagag cgtaatcgtg agcaaaaagc
    15901 acgactgtcg gaacaatcca aattagaagt ccaaaaagac cgagctgaaa tgattttaga
    15961 taatcatttg acctatctgc agtcagaata tcagctgaca tttgagaaag cttcccaaga
    16021 ctatcaagaa acaaccgata tcgaagacag tagaacaaag gtttcatcct taaaagaaca
    16081 gattgaaaaa ctgggaccag taaacttaaa ttcaatcgaa caatacgaac aagtcagtga
    16141 aagacataca ttcttagcaa cacagcgaga tgatctgtta gcagcgaaaa accaattgtt
    16201 tgaaacgatg gatgagatgg atgatgaagt gcggacaaga ttcaaagaag tatttgaagc
    16261 tatccgtcaa gaattcaaaa ttgtctttcc aaatatgttt ggcggcggta aagctgaatt
    16321 agtactgacg gaaccaactg atttgctgaa aacaggtatt gagattgaag tacaacctcc
    16381 tggcaaaaaa ctgcaaagtc taagtttgct ttctggagga gaacgagcac ttacagccat
    16441 cgcgttgctc ttttccatta tccgtgtctg tcctgttccg ttttgtatct tagatgaggt
    16501 agaagcagcg ttagatgaag ccaacgtgaa acgttttggt cgttacttga gcgatttcca
    16561 agatgacaca caatttatcg tagtcacaca ccgcaaagga acgatggtcg cagcagatgt
    16621 actgtacgga gtgacgatgc aggaatccgg tgtatcaaaa atcgtttctg tacggatgga
    16681 agatatcaat gaagaggcga aaatcaacga aacgaaagtg taggaacgaa aatgatcaaa
    16741 ttagtagcaa tcgatttgga cggtacatta ttaaatgaac aaaagctgat ttcagatgag
    16801 aataaacaag cattagcaca agcaaaacag caaggggtca agatcgttct atgtacagga
    16861 cgccccttag ctgcgatggc gcattatctg caagaattag gactggtaga tgaaggtgac
    16921 tacagcatca ctttcaacgg cggtttggta caaaaaaatg atactggtga aatcatggaa
    16981 aagaaagtga tggaagtatc agatatccaa cgcttataca aactggctca gcaattggat
    17041 ctgccactag atgttttgtc tgacaatgtg gttttacagc tgcctagtgc cccaaggaag
    17101 caatccttgt acaatgtgtt gaataagctg ctgcaattcc aaccagcgga tataagcgat
    17161 attacagatg aatttgtgct taataaggca gtaatcgctt atgaccaaga agaattagat
    17221 cctaaaatca aagaaatacc agcagaattc catgaaatgt atgagatcat caaaacaaga
    17281 agtatgcttt tggaatttat gccaaaaggt gttacaaaag cttatggtat ttctttattg
    17341 gcaaaagatc ttggtttgaa acaagaagaa atcatggcga tcggtgatga ggaaaatgat
    17401 ttaccgatga tccaatatgc tggactaggc gtagcgatgg agaatgctgt gcctttcgtc
    17461 aaagaagcag cagatttcgt gacagccagc aatgaagaaa atggcgtagc caaagtgatc
    17521 caaaaatttg tattagagta gggggagtca aatgggattt tttgataaaa tcaagcgtgc
    17581 ttttactggc gaagagaaaa cagaagaagt tgaagtacag gagaagtacg acaagggttt
    17641 agaaaagacg cgtaaaacat ttggtgaacg gatgaatgag ttgtttgcca attttcgaac
    17701 agtcgatgaa gacttttttg aagaattaga agaaacactt atcggtgcag atgtaggttt
    17761 tgaaacagcg atacgaatca ccgaagcatt acgtcaagaa gtgaaactca gaaatgcgaa
    17821 aaaatcggca gaagtccaaa atgcgatcat cgagaaactt gtggatctct atgaagcgga
    17881 aggaatcaat gaagtaaatg aactaaatat ccaagaagac ggactaacag ttattttgtt
    17941 tgtcggtgtt aatggtgtag ggaaaacaac aagtattggt aaacttgccc atcagttcaa
    18001 aaacgaaggt aaaaaagtat tgatggcagc agcagatacc ttccgcgctg gagcaatcga
    18061 ccagttagtt gtctggggcg aacgcgctgg tgttgaagtt gttcgtggca atgcaggagg
    18121 cgatccagca gctgtcgtat tcgatgcttt ggaaagagca aagacagagc aggcagatgt
    18181 tttactagtt gatactgcag gccgactgca aaataaagtg aacttgatga acgaattaga
    18241 aaaaataaaa cgagtaatcc agcgggaatt accagaagca ccccaagaaa cacttcttgt
    18301 attagatgca accactggtc aaaacgcgat ggttcaagca aaacaattca aagaaacaac
    18361 agatgtgacc ggtcttgttt tgacaaaatt agatggtaca gcaaagggcg gtatcgtact
    18421 tgctatccgt aatgaattgc atttaccagt gaaattggtc ggtcttggtg aaggaatcga
    18481 tgacttggag ccgtttgatc caaatgattt tgtcgttgga ttgttcaaag gattactaaa
    18541 agaagaataa cccatgtcta aaaagcagga gctgtgacaa aagttgcagc tccttgtttg
    18601 aatgattgtt tttaacatag ctgtgttttt gatgccttca tttttatact tatttgtaag
    18661 cggttctcaa acaatttcgt tctttttctt agactttttg attataatta gattcatatg
    18721 aattggaggt cacttaatga tgaattttaa gcaattatta ttgcatgtca atgcgcgccc
    18781 ttttatcgac caagctcgat ttggtataga acgggaagga cagcgggttg atcttgcagg
    18841 aaatctagca aaaaccgatc atccagcaat ctttggcgat cgatcctatc acccctatat
    18901 ccaaacagat tttagcgaaa cacaaacaga gatgatcacc cctgttaccg attctattcc
    18961 cgaattattt cagtatctcg ctgctgttta tgatgtgact gctcgttcta taccgaaaga
    19021 agagatgatc tggccattaa gtatgccacc tgccttaccg gaaaaagacg aagagatcat
    19081 tattgcaaaa ttaaaaaatt tcgaagatgt cttgtatcga cgttatttag caaaaaaata
    19141 cgggaagcga aaacagatgg taagcggtat tcatttcaat tttgaatttg gcgatgagtt
    19201 gctaagaaca ttgttcagcc atcaggaaga atttcaagat ttttccgaat tcaaaacaga
    19261 actttatttg aaaacagcga gaaactttat gcgctatcgc tggatgatca cttatttatt
    19321 cggtgcttca ccaatgagtg agaagaacta ctttttagat gaatcacatc cgcaagaacc
    19381 tgttcgcagt attcgcaaca gtgcgctggg ctatacgaat catccaaatg tgaaagtttc
    19441 ctatgcttct atgaaacagt atttagcaga tatcgagcga atgatcgaag aagggaaact
    19501 ttcggaggag aaagaatttt atacaccgct tcgtttccga ggcggaaaga aagtcgcaga
    19561 tttagcaaca acaggtgttc gttatattga attgcggaat atcgatttga atccttatgc
    19621 aagattgggt atcaatccag agcaagttcg gttcttacaa ttattcctga tgtacatgtt
    19681 atggacagaa gaaaaagaag actgtgacca atgggtagca gaaggtacga ctcgaaataa
    19741 caaagtagca ctagaacaac catcagatca aacagaattt catcaagaag gtagagaaat
    19801 tcttgaagga atgaagcaaa tgctggttga attggattgg ttggattctc tttatttagt
    19861 agaggaagca ttgactcaaa tggatcatcc tgaacaaacg ttagcagcga aactctatca
    19921 agaagcacag ctatcgagtc agcaagaagt tgctgtggca ttgggacatc aatattataa
    19981 agaaagccat gaacgtccct atcagttggc cggttttcgc gaaatggaac tttctactca
    20041 gatatttatg ttcgatgcca tccaaaaagg tgtccaagtc aaagtattag atgagtcgga
    20101 tcaatttttg cgtctgcaat tccaagacca tgtagaatat gtgaaaaatg caaacatgac
    20161 aagtaaggac agctatatcg tcccacttat catggaaaac aaaacagtaa cgaaaaaagt
    20221 cttaaaagaa gctgggttcc gggtaccagg aggcgcagaa ttttcatcca tggaagaagc
    20281 ggtaaaagct tatccgagat ttgccgagca ggcatttgtg atcaaaccta aatcaaccaa
    20341 ttacgggtta ggtatcacga ttttcaagga aggagctagt ctggaagatt atcaagcagg
    20401 tttagccatt gcttttcgtg aagacagttc ggttttagta gaagaattta tgccgggaac
    20461 ggaatatcgt ttctttgtga tcgacggaga agtccaagcc atcatgttgc gagtccctgc
    20521 taatgttatt ggtgacagta tccgaacagt gaaagaactt gttgaagaaa aaaatagtga
    20581 ccctttgcgt ggaacgaatc atcgtgcacc tttagaattg atccaattag gtgaattaga
    20641 acaattgatg ttgaaagaac aagggttgac gatcgaatca gttccccaag ccaatcaaat
    20701 tgtttatcta agggagaact caaatatcag tacaggtggt gattcgatcg atatgactga
    20761 tgaattttct gaagcttaca aaaaaatcgc cgtttctgcg gtagaagcac taggagctaa
    20821 gattagtggt atcgatttga ttataccaga taaagaaatc gatcctacaa ctgataaaaa
    20881 agcatatggg atcatcgaag caaatttcaa tccagccatg catatgcatg tttatccttt
    20941 tgcaggaaaa ggcagaagat tgacgatgaa tgtattgaaa ttgttatatc ctgaagtatt
    21001 ttaaaagaaa aagcttttga cagattgtcc tatttgatgt caatctgatc aaaagctttt
    21061 tttgcatgta aaaatttatg tgttttttcg ttttaaaata aacgatccca gaagcttttt
    21121 ttcttttttg gtttttcttt ttcagctggc gaagagtttt cttctgggta ttttttttcg
    21181 atatcaatca caggttcatt tactgcttct tttgctgtta agagcaatcc gaattcctca
    21241 ggatttgcag tgacgtaatc gttgactaca gtgaaacggc tattgttttt cgttgctagc
    21301 tggatatatt ggttttggac agaaaggggt agagccccat tcaagagtat ggaagcatct
    21361 ggatgttttt tgagttcttt taaaaagttt tctttgtttt ttggttggat catttcagca
    21421 acagtcatac taagatagca gcgttccctg aatgtcccta aatatttatg ctgttcttca
    21481 gggttcacta aaggtgtacc ataaagtccc ttatccaaat ggctttttac atcatcttgt
    21541 gccataaaaa ataccctcct tcttctcttt gtctttctta cgattatagc atattcccaa
    21601 taatacctaa aacatatgaa aaagcggttg cttttgttca acgattcact taaaataaga
    21661 atgaaaatca ttctcaatta gaaaggtgga ttggatgcgc tacggacgac tagtagcatt
    21721 attagttatt ttaagtagta tttcattgtt catcggagta tcgaatgtac aattgtcaga
    21781 actgctccag ttagatgggc aaagcttaca aatcttgcta gtgactcgtt tgccacgaac
    21841 gatttgtttg atccttgtgg gagcgaccag tagtatttgt gggttgatca tgcaacattt
    21901 gacccaaaat aaatttgttt caccaacgac tgcgggcacg atggatagtg cgcgtttagg
    21961 tattttagtg gctatgattt tcctacctgg tgcatccttg attgtccgat ccttgacagc
    22021 attttgcttt gcttttgcag gaacattgct ttttttatcc cttacgcgat tgttcctcca
    22081 gaaaaatcaa ttgatgcttc cgttgattgg agtcatgtta gggaatatca ttggttcagt
    22141 tgctactttt tttgcttatc aattccagtt ggtacaaaat atgacttcat ggcttcaagg
    22201 gaattttgcg actgtgatac gaggaaatta tgagttattg tatgctgttg ttccattgtt
    22261 gctcttggtt tggctatttg cttaccgatt tacgatcatt ggtctgggcg aatcgtttgc
    22321 agtaaatctg ggaatagatt accagaagat gcagttttta ggcattggct tagtatcatt
    22381 ggctagtgca gtcatgttga tcatggtagg aagcattcct tttatcggag tgatcattcc
    22441 gaatcttgtc tctagaatat atggagatca agtgcacaaa acaattggcg taactgcttt
    22501 agcaggtagt ttgttcttag tattttgcga tcttgtcgct cgcgtgttga tttttcctta
    22561 tgaaatacca gtcagtgtga tcgttggaat catcggtggg gctatcttcc tttatcttct
    22621 gataagggga cgacgatatg cttaaaaaaa agccatctat ttttattggt atgcttgttc
    22681 ttgttttatt tgttacagga atgtacttga cgatccatac atatggaaac tggcattttg
    22741 catgtgcatt gaggagtaag aaaatttttg catttatcct tgtctcgcta gctgtcagca
    22801 gtgccacaat cagctttcag acaattacgc ataaccagtt tttgactcct tcagtattgg
    22861 gacttgagtc gttatatgtc cttgtccaaa cggtcctttt cttctttatc ggaggagtga
    22921 cgatgcttca gcagacaggt acagtttttt tcttattgaa cgttgggctg atggtcgggc
    22981 tgagtctatt gttatttttc tttcttttga aaaaaatgag agcgaatctt tttttattac
    23041 tgatgtcagg gatgatttta ggtacgcttt ttggtagtgt cagtaccttt ttacaagtca
    23101 tcatggatcc gaatgaatat gatttgcttc aaggcagatt atttgcaagc tttggcaata
    23161 tccagagtca gtatcttttg cctgcttctt tgatcattat cggctgctgg tacctgttat
    23221 ttcgatatcg acatgtgcta gacgttttcc atctcggaaa tgaacaagca gtaaacctcg
    23281 ggatagacac agatcgtttt gaatttttga ttttatgttg tgtcagtctt ttgacaggga
    23341 catcaacggc tcttgtgggt ccagttactt ttttagggtt tattgtcgca aatatcagtt
    23401 atcgtgtctt ttctacttat cgtcatggtc agctatttat cggaagcttt ctggtagcta
    23461 tgctgtttct gacaggagga cagctcgcag tagagcagat tttcggctgg aatacaacag
    23521 tgagtgtcgt cgttgaattt gtcggaggaa tttattttgt cacaaaattg attcgcgaaa
    23581 ggaagagtat cctatgattg atttacaaaa ggtcaataaa ctgtaccatg gaaaatcagt
    23641 ggttaaagat gtacagttaa cgattcctga aaagaagttg actgctttta tcggtccaaa
    23701 tggtgctgga aagtcaacgg tgttgtcgat gatcagtcgt ttgataccaa aagatacagg
    23761 tgaaatctat ttagaccata acgaagtaaa ggcttggaaa tccaatgaat tagccaaaaa
    23821 attatcgatc ctacgacaat cgaatgaaat aaatgtccaa atcaccgtga aagaattagt
    23881 agaatttgga cgatttcctt atacgaaagg aaaattgacg acaaaagacg aggaaatcgt
    23941 tcaggaatca ctggaacacc ttgggttaga agaaatagcg gatcaatcga ttgacactct
    24001 ttcaggcggt caactccaac gtgcgtatat tgcaatggtg ctagcgcaag acacggatta
    24061 tattttactg gatgaacctt tgaacaatct agatatgaat tttgcggttc aaatcatgca
    24121 gattttaaaa aatttagtac aagaaatcgg gaaaacaatt gttattgttc ttcatgatat
    24181 caactttgct gcaagttatg cagacgaaat tgttgcaatg aaagatggac agttatttac
    24241 gcaaggaacg acccaagaaa tgataaaaaa agatgtattg gatgaattat acgaaatgga
    24301 catacggatt tgtgagattg aaggccgcag attctgtctt tatttctaaa actaacatta
    24361 ggagagaaga gatgaaaaaa ttagtagtag gtatcttgat tctagcatca tttggtttag
    24421 cagcatgtgg aaatagcaac aacaccagtc aggcagacac aaaaaaaagc agcacacaaa
    24481 ctgaaactac attgacgata acagatagta atggagacca aatcgaggtg ccaaataatc
    24541 cagaaaaagt cgtcgtattc gacaatggtt ctttagatac gatggatgct cttggtgttg
    24601 gagataaggt agttggagca gctacaagca gccttcctga gtatctttca tcctataaaa
    24661 aagtagaatc agcaggtgga ataaaagaac cagacttaga aaaaattaat cagcttcagc
    24721 cagatctaat catcatttct ggccgacaaa gagattttca aaaagatcta agtcaaattg
    24781 ctccgaccat tttcctttct ttagatgcga aaaatccatg ggaatctttc cagcaaaatg
    24841 tcactgcttt aggggagatc tttggtaagc aagaagaagc taaaactcaa ttagaggaat
    24901 tatctagtgc aatcgatcag acgaaaaaga aagcagaagc tactgacaaa aaagcactag
    24961 tcacacttgt gaatgaaggg caattatcag cctacggatc tggttctaga ttcggtttca
    25021 tccatgattt atttggcttt gaacaagcag atgatcaaat cgaagcatcc actcacggtc
    25081 aaagtgtttc ttatgaatat gtgctggaaa aaaatccgga tatcttattt gtagtagaca
    25141 gaacaaaagc tattggtgga gacgacagca aagatgatat ttctgccaat gaactggtag
    25201 cacaaacgaa tgctggtaaa aatcagcaga tcatctccct tgaaccagat gtctggtatt
    25261 taagcggggg cggtttagaa tcaatgaaac tgatgatcga agatgtgaat caagcattta
    25321 aatagcataa ataacagagg attaaactct catactatgt aacaataatg tttcttacat
    25381 tcttaaatag caagagccta tcctcaaaga agttatcaaa aaccatccgt aaaatcgtat
    25441 tgatgatttt acggatggtt tttatttatt tgagaaatct tttttcaacg tgaattcatt
    25501 atctacatca tacgggttga ttctagaatg gggagtggca aaaaatagtg tgatccattt
    25561 tttcagcatc aagatcgcct cctttgattt gaatcaaagg tacaaagcga aacttagata
    25621 aaacttaaat ttagatattt ttattttgta ccgatctctt ctttaaacga tgttccatct
    25681 gctgcttctc ggcaataagt ccactagact agaaaatatg agaagctatt ttctgtccag
    25741 cgttcttatt actcaaaaca aatcgcagtt gtcacaatct cttctaaacg atgttccatc
    25801 tgctgcttct cggcaataag cccactagac tagaaaatat gagaagctat tttctgtcca
    25861 gcgttcttat tactcaaagc aaatcgcagt tgtcacaatc tcttctaaac aatgttcaat
    25921 cagctgacct tcggtattag ctcaaagcac tgatttcaca acctctattt aggcatgtgt
    25981 ctcagcaagc ggttgatagt tgatgatctg catttttcga taaaaatcgg cgggaagctt
    26041 actccaccgt tttagtaatt gatagaaata actctcttct gctttgattt tatactcgct
    26101 taatatcaat ccaaaactat tcgttttttt tgaagctaat tttttataaa tatccaatgc
    26161 tttggtttta tcttcattga caaagagtgt ttttacttca atataagaac cctcgatatt
    26221 ttgtgtttct tcgataaaat agatgttttt tatatattcc ttcatagtga ttcctccaaa
    26281 gaagtctttt atttgataag tttactctac cgaagagttg ttaataatcc gtgaacaaaa
    26341 agtaattctt tttccaatag ttgttaaagg ttttttattt tttatagtat tttttgttat
    26401 ttaagaaagg aataaggtta aacaaataac aggttgcttt ttgggtcttt ttccgttcaa
    26461 gaaactgaac gaagaaaagg gaaagttgtc tcttttgtga taagagaaat cataataaac
    26521 gaatgactta ctttccaata aattgagaac agtcagaacc agacaaatta gttgcatctg
    26581 atgaataaag tgattattct atgacatgag gtgatacaga tgaaattaga agtgacaaaa
    26641 gaagcacaag aagtattgaa gaataaatta gaagcagacg atcaactttt attagatatc
    26701 gaaaacggtg acggtccatt tgcagaaagc caagtcagtt gccaattgga tacggcattt
    26761 cgattgatca ttgtcaaaaa agatacacaa acagatctaa gtctttattc tgtagtggct
    26821 gatacaccag ttggaccaat caaaatcaaa gacagtgcaa tactttacat ggatgatcca
    26881 tccacacttg ctttagaacc aacttataat agtttacaat taaaaggacc aagcggcttg
    26941 agaaaaggca acttgcaagt tgttcgaaaa gactagaggg ataaaatggg aaaattggcg
    27001 atcatcagcg acctacatgt ggacatcaat aaattcggcg aatcagagct gatgcagctt
    27061 tggcaagttt tgcaaaagca caagatcact cggcttcatc tagccggaga tacggctaat
    27121 aaagtagacc gttgcctagc tgttgtcgaa tttttcgtca ataaaggatt atctacgaca
    27181 tttcatttcg gcaatcacga gttggcagat gtgcccaatg aacagatgat ggaccatttt
    27241 ccagacccac attttttaaa tgaacggtat attgctttaa atgagcagac cgttctttta
    27301 gggatgaatg gctggtacga ctatcagttt tccgaattga aagattacga ggcaatcgta
    27361 cgaatgaagc gtttgtattg gtatgatcgg ttcatccagc gtgggaaaac agatctcgag
    27421 gtcaatgcag aaatgcttgc agctgcaaaa aatttgctag accagctgga agcaaaaaaa
    27481 cttgaggtga ttttatccac tcatttcgtt cctaaacggg catttatcat gtatcaatct
    27541 gctccttatg agcgttggaa caaattaaat gcatttttag gatcagaatc attcggagaa
    27601 ttacttgacc gttattcaaa tatcaaacaa gtagtttttg gccatacgca tcgtcgcttt
    27661 gaagataaat ccatcggtca cacgatttac agctgcagac cttttggtta ttattatgaa
    27721 tggcagctga cccgagattt tgtcttgaaa gaacagttgc tagacagcta tgatcctatg
    27781 aaattacgtg tcctcttgcg tgcccatcac ccagcttttc aggcctatca aacacaacat
    27841 ttgcaagagg aatttgaaca agcgatgaca atcgttccgt attgagacag cattagtaat
    27901 gtgtaaaaga aaataccttg taaaacataa ggataagtaa gagcagtaga tccatttatc
    27961 gtggatatgc tgcttttttt gcggaaaata atcagcgaat gtttatttga tcggatgatt
    28021 tgtaactcgc agagtactag atgcaagcgt gtattctttt tttgaacgat aatcacaaaa
    28081 aaaaatataa tatacatttt ttatttaatt ataattaata ttgattgaag tctaggcagt
    28141 caagtcacat tttgatagat aggaggggga cttacgaatt attttaaagg ctatgacagc
    28201 tgtatttcta gattgtgttt gcatcaagtt gcaataaaaa ctagaaatca aatcgggctg
    28261 ataatcattt ataactaatc gatacatagg accaaatata ggtctatgtt cttttatttc
    28321 ataagtatcg taaaacagaa ttttgctatg gattaatcca atcgactgat atgtttagta
    28381 aataaagaaa gaggaatgtc aatgatgaaa aaagaaatcg tattaacagc agcaatgatg
    28441 ttattcagca tggtagcaag tacgactttt gtttctgcaa ctgaagtata cccaaaggaa
    28501 tataacacag aaggaacgat tacatttgaa gcaggagatg agggtgtaac tcctcctgtt
    28561 gatccagaga atcctgatcc caataaacct gtcgatccat cagatcctcc aagccctgga
    28621 acaggagggg cattatcgat tgattatgga tcaaaattca aatttgggac tcaaaaaatt
    28681 tctactgcgg ataaaacgta ttatgcagct gctgatgtga tgaacgatgg gtctagaaaa
    28741 ccgacatatg tccaagtgac agataggaga agtaccttat ctgggtggaa attaagtgtc
    28801 tctcaaccag aacagtttaa aactgccagt ggggatgaac tagttggagc acagctgaaa
    28861 tttacgaaag ggcaggctgt ttctttggtt gatccaacat atacgccgca aacggtgaat
    28921 tctgaattaa cgttgacacc gggaggaaac aatacgttag caattaatgc taaatcagga
    28981 acaggtgtgg ggacttgggt ctatcgattt ggtgcaaatg aaaatgaaaa tcaagatgcg
    29041 gtacagcttt cggtacccgg taaatctgtc aagttagcgc aacaatattc cactaaatta
    29101 gtttggacat tagaagatac acctaacaac tagtttactg aaaagaactg ttaaaaataa
    29161 atacgtagtc atattgtctt gcgaggtaat cgagcaaaaa ttgtcaaagg ctaggctaaa
    29221 tttgtttgga tacgaatagt tcatctagga atttaacgat caggaggaaa agatatattg
    29281 aaaaagagga ggatcgtgct cattttaagc agtctcttag tgactaatat ctttccgatc
    29341 atagggtact ctttggaatt gaatcagttg gagcatggaa atagaagaga cgaagaaagc
    29401 aaactcactt cgacattcga cagtttaata gaatccaacc agacacaaga aagcagtgag
    29461 gatttaagta tttcgagtag tggagagacc actgacaacg tagaagctga gggttcggac
    29521 aaacaaggac tagaagatgc accaatttct gcggttgagc tgcctgacga tcccaatatt
    29581 atttctatcg ataaaatttt tcaaacacct atcggtacta gcacgtccat attggaaaat
    29641 gggaagttac tgcagttgaa cccagcagaa aggtctcaaa gaggagctat ttggtcaaat
    29701 aagaagatta gtttactctc ggattttaca tttaaatctt atttatactt aggaaatcaa
    29761 cgagatagag cgggagatgg catgaccttt acattgactg gcgatcctag aatggctagt
    29821 aatcccagtc aagttattgg atcgccaggt atgggaattg gctcatattc tactcgttct
    29881 ggccaacctt acattgcgaa tgcgctctcg attgagtttg atacgtataa aaatagtggt
    29941 tcttctgatc gaatggatcg agagataact gctgataata gagggcatgg acatgtcgct
    30001 tttgtcacgc ctaaaccaaa taataataat tattctggag agcatactgg cgtgacggta
    30061 gctcctacat ttctttctaa tggcacatgg cgcttgttaa taattaaatg ggacgcggag
    30121 caaagaagtc ttacttatga tttggaaggt gtcggtcgct cttctcggat tatacctgat
    30181 ctcattaggc agtttggttc tacagaagta aattgggggt ttacttcttc aacaggtact
    30241 ttttttcaag aaaatgctgt cgctgtcacg cgacttccat cgaatgtcac atcaacagct
    30301 gatgtaaaag tcaacgatag tgattatggg gccatcgctg aagctggagg aaacgataag
    30361 ataactatca gaaatacatt gaaggtcgag ggggattttc ttgaatataa gaaaatgcag
    30421 actagtatcg acctcccaca agaattaact attcccgagc atatcttgat caatggaaat
    30481 caaattgatg ccaaagatat cttgataacc ggaaatcgag tcacgctaaa tctgacagat
    30541 tattttattg ctggagatac gttaataatc gaattggaga caactttgca ggaagtaatt
    30601 cctgaaaaag tactcactat gcattttgaa taccttgaaa acgggaattt gattgagcaa
    30661 tcgaatgaaa taaacctaaa gatcgcaaag ctgaaggaga tagatatcca tgtttattat
    30721 aaagataaag acagtcagat tgatattgct gaaatgaaaa caatcactgg gaaaatagga
    30781 gagaagtata aagaaactcc tattgatatt gaagggtatg tttttgactc ggattcagcg
    30841 aatacagaag gcatcttctc cgaaaataca gaggatatct atttttatta cagaaaaggc
    30901 aaactttatc tctcaactgc tcctcaatat gtcaattttg gaaaacataa aatatccagt
    30961 agaccattaa gtgaattcgg acagttttca ggtgggttga aaattataga tgaacgggct
    31021 tttggaagtt ggaaattaca attgaaacaa gagaaagttc ttacaaatgg agattttgag
    31081 cttccagaag ctctttcctt tgttacctct gaaaatacaa cggttatagg gacatccgct
    31141 gttacgattt tcgaaagtga tcaaaagggt gaaagcgatt tgtcctctct tttagattct
    31201 acagctcatc gtggaatcag aataaatatt cctgtagaaa accaaagaat cggaaccttt
    31261 gaggggaaac tatcttggat acttgcagat gtcccaggaa attaacagga agagaagaat
    31321 ataacaaaat tattgagttt caagaagggc acaggtacca taggctcttg tgtcccacag
    31381 agacaagatt ttgttatagt cttctcttac atatgattaa aggagcgatt ttcttgagac
    31441 caaaacaaca attatttata acactcctag ttattttagg ggcacttttt aacaatatat
    31501 cagtgtcagc tagtgcattt aatttttcag tgacgccgat aacttcagag aatcaaatcg
    31561 acaagagaaa aacttacttc gatcttcaac ttgatccgga tcaggaagta gaagtaaaag
    31621 cggaattgag aaatgataca gaaaaagaag tgaaaatcga tatttctgtg aatagcgcaa
    31681 ccaccaattc aaatgtgatg gttgaatacg gaaaaaacga gattgaaaaa gacatgagcc
    31741 tgatctttga tctggtagat tatgtgagct atccacagat agtgacgcta aaacctaaaa
    31801 gtgtccaaac cgttgctttt catgtcagga tgccgaatga gcgatttgat ggtgtattgg
    31861 ctggtgggat cacattccaa gaaatccaga cggaaaagga ccaaacagaa actaaggatc
    31921 aaagcttatc gattgagaat gaatacacct atattattgc tttgttgatg caacagacac
    31981 tgaacgaagt tgctccgaac cttactcttc atgaagttaa accagatcaa atcaatgcaa
    32041 gaaatgtcat cttagcaaac gttcaaaatg atcaaaaaac ctatatcaat caagtagtta
    32101 ttgaaacaaa aattacaaaa aaaggacatt ctgaagtttt atatcaagag gaaaaagaag
    32161 gattgcagat tgcgccgaat acaaattttt cttttccaac tgctttaaat ggtcaaccgc
    32221 taaccccagg tgaatatcac cttacaatga ctgttttagg aaatgaaaat gaaaatggaa
    32281 aattttcgag aaaaaaagga aatactacga tcaactatac caatcaatgg gtatttgaaa
    32341 aagattttac aatagacgga aaagtagcca aggaattgaa cacgacggat gttacgatta
    32401 aaaaagacaa taattggatt tatatcttag tagcttcact tttcttattg tttattttat
    32461 tatgcatact tttgtttgtt ttgagaaaaa aagaagagaa agaaaagtaa ggttaagata
    32521 acaaatagta aatttttatt attgtaggat ggttgggctc tttttataac aatataaaat
    32581 ataccatttt attaataaaa atgcatacta gtaaatgata taattctaat aacaggtttg
    32641 atcgcgagaa aggccaaata aaacgctctc caaaatgtta aaaaaaagac aggacgatgt
    32701 atattataca aacaagaatc gctgacaaca gtgactcgga tccaggaaat cagttttatc
    32761 cccagctagt tggtgaatgc atggaaagta atatacgtag aaagagctga tgactaatga
    32821 attatgagtt ttttttaagc aaagaagatc acagaagtta ttcattactt aaatatttag
    32881 aagcaagctc aaatttatca gagtcaatat cgaatgttca agaagagttg tcgctatcga
    32941 catttctctt gaaaaaaacg atcgataagc tgaaacatga tttacaaaag ttcgatctag
    33001 agaaaaattt gcgagtaacc gtcagtgaat tggatatatg tctggaaatt aatggaaaat
    33061 gttctagcaa agctctgtta tctcgttata taacggattc tttatctatg aaaatggtat
    33121 tggctttctt tcaggaaaaa tatatctcag tagaaaattt tgctaaacag catcacgtga
    33181 gttattctgt ggcttataaa gtattacaaa gactaaaaag aaatctaaaa aaatatcaaa
    33241 ttttttttga aaagagaaag tt
//
LOCUS       DAFCNS010000029        32909 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFCNS010000029 DAFCNS010000000
VERSION     DAFCNS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.029"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            132..875
                     /locus_tag="LNE23_001250"
     CDS             132..875
                     /locus_tag="LNE23_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931119.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            881..1245
                     /locus_tag="LNE23_001251"
                     /pseudo
     CDS             881..1245
                     /locus_tag="LNE23_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1261..1791
                     /locus_tag="LNE23_001252"
     CDS             1261..1791
                     /locus_tag="LNE23_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931120.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            1953..2261
                     /locus_tag="LNE23_001253"
     CDS             1953..2261
                     /locus_tag="LNE23_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931121.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            2257..2676
                     /locus_tag="LNE23_001254"
                     /pseudo
     CDS             2257..2676
                     /locus_tag="LNE23_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2813..3613
                     /locus_tag="LNE23_001255"
     CDS             2813..3613
                     /locus_tag="LNE23_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931122.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            3650..3832
                     /locus_tag="LNE23_001256"
     CDS             3650..3832
                     /locus_tag="LNE23_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931123.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            3943..4260
                     /locus_tag="LNE23_001257"
     CDS             3943..4260
                     /locus_tag="LNE23_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931124.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            4263..4495
                     /locus_tag="LNE23_001258"
                     /pseudo
     CDS             4263..4495
                     /locus_tag="LNE23_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4610..5089)
                     /locus_tag="LNE23_001259"
     CDS             complement(4610..5089)
                     /locus_tag="LNE23_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931125.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            5209..6546
                     /locus_tag="LNE23_001260"
     CDS             5209..6546
                     /locus_tag="LNE23_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBK6931126.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            6569..7096
                     /locus_tag="LNE23_001261"
     CDS             6569..7096
                     /locus_tag="LNE23_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931127.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            7083..7646
                     /locus_tag="LNE23_001262"
     CDS             7083..7646
                     /locus_tag="LNE23_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931128.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            8068..8805
                     /locus_tag="LNE23_001263"
     CDS             8068..8805
                     /locus_tag="LNE23_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931129.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(8822..9238)
                     /locus_tag="LNE23_001264"
     CDS             complement(8822..9238)
                     /locus_tag="LNE23_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931130.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            9631..10878
                     /locus_tag="LNE23_001265"
     CDS             9631..10878
                     /locus_tag="LNE23_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBK6931131.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            11146..13536
                     /locus_tag="LNE23_001266"
     CDS             11146..13536
                     /locus_tag="LNE23_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBK6931132.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            13585..15420
                     /locus_tag="LNE23_001267"
     CDS             13585..15420
                     /locus_tag="LNE23_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK6931133.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            15489..16352
                     /locus_tag="LNE23_001268"
     CDS             15489..16352
                     /locus_tag="LNE23_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6931134.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            16342..17451
                     /locus_tag="LNE23_001269"
     CDS             16342..17451
                     /locus_tag="LNE23_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6931135.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            17790..18590
                     /locus_tag="LNE23_001270"
     CDS             17790..18590
                     /locus_tag="LNE23_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931136.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            18678..19484
                     /locus_tag="LNE23_001271"
     CDS             18678..19484
                     /locus_tag="LNE23_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931137.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            19716..20585
                     /locus_tag="LNE23_001272"
     CDS             19716..20585
                     /locus_tag="LNE23_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBK6931138.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            20605..20952
                     /locus_tag="LNE23_001273"
     CDS             20605..20952
                     /locus_tag="LNE23_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931139.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMVIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            20965..22227
                     /locus_tag="LNE23_001274"
     CDS             20965..22227
                     /locus_tag="LNE23_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931140.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            22307..23683
                     /locus_tag="LNE23_001275"
     CDS             22307..23683
                     /locus_tag="LNE23_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBK6931141.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            23680..24309
                     /locus_tag="LNE23_001276"
     CDS             23680..24309
                     /locus_tag="LNE23_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931142.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            24321..24791
                     /locus_tag="LNE23_001277"
     CDS             24321..24791
                     /locus_tag="LNE23_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931143.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            24788..25270
                     /locus_tag="LNE23_001278"
     CDS             24788..25270
                     /locus_tag="LNE23_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931144.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            25267..25929
                     /locus_tag="LNE23_001279"
     CDS             25267..25929
                     /locus_tag="LNE23_001279"
                     /inference="COORDINATES: protein motif:HMM:NF019467.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290663.1,RefSeq:WP_010747081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBK6931145.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            25916..26284
                     /locus_tag="LNE23_001280"
     CDS             25916..26284
                     /locus_tag="LNE23_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HBK6931146.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            26365..26817
                     /locus_tag="LNE23_001281"
     CDS             26365..26817
                     /locus_tag="LNE23_001281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931147.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRIRITEQAIEKQKDKEKKEIMSIYLSSGASFFHE
                     IDSMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            27469..27522
                     /locus_tag="LNE23_001282"
                     /pseudo
     CDS             27469..27522
                     /locus_tag="LNE23_001282"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27526..28125
                     /locus_tag="LNE23_001283"
     CDS             27526..28125
                     /locus_tag="LNE23_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBK6931148.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGLLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIRFDSKEHLLLSVFILLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNISLIKQKN"
     gene            28141..28617
                     /locus_tag="LNE23_001284"
     CDS             28141..28617
                     /locus_tag="LNE23_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931149.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            28634..29536
                     /locus_tag="LNE23_001285"
     CDS             28634..29536
                     /locus_tag="LNE23_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931150.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            29609..29800
                     /locus_tag="LNE23_001286"
     CDS             29609..29800
                     /locus_tag="LNE23_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931151.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            29964..30551
                     /locus_tag="LNE23_001287"
     CDS             29964..30551
                     /locus_tag="LNE23_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931152.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            30551..31450
                     /locus_tag="LNE23_001288"
     CDS             30551..31450
                     /locus_tag="LNE23_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBK6931153.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            31450..32364
                     /locus_tag="LNE23_001289"
     CDS             31450..32364
                     /locus_tag="LNE23_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931154.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            32416..32631
                     /locus_tag="LNE23_001290"
     CDS             32416..32631
                     /locus_tag="LNE23_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931155.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT        12443 a         4451 c         6663 g         9352 t
ORIGIN      
        1 gatctttagg gcgtaagccc tattttttta aaattaagtg ataacaaata aatgatttct
       61 gaaacaaacg ttcttactca actactcgaa acacacaagc taattaacta gattataaag
      121 gagaatgcgt aatgaaagaa atcagtgcaa atgaagctca tattccgcaa aagaatcagg
      181 ttggtatata taaggtgctt catggtgaag gaatttataa aatacagtta cttaccggtt
      241 acaataatgg tgcaaatggg aagtggttaa aggcaacaag tattcgtgaa cttagaagtg
      301 aagtagaaaa aattaggctt ggtctcacaa gtgaagaaat agctaaactg aagggaaata
      361 attcttttgt aattgaaaaa gaaaaggaaa aacatctact ttatcgtgag ctgaaaattg
      421 aagaatacta tgaaattgat ttacctgaaa aattaagaat tattgatcaa cgttcagaag
      481 aaaaatggaa aactgaaata tatccaagct taaatgacta caagacatca ggaaagccca
      541 tgtataacta cacaggcttt ttaacctaca cacaatacaa atatgccttt cgcaaattaa
      601 aaggcattaa acaagctgaa attgccctgt atgagatgaa aaaagtggat agattgaaat
      661 tgattcaaga tttagtgcaa aatacatgga atcaagcgat tgtcgagcta gaaaaagaaa
      721 atgggaaaat tttaactaat aaatatctga tcgtaaatga aattgaagaa ttaattggtt
      781 tattttcatg tgaaaaaggt gaagtcttct tgaagtcatc accaacctat tcaattttgg
      841 acagtccgta tcagtttata caggcaagga gatgagttag atgtctagct tgaaagatga
      901 ctatatccaa gcaaaacaag agtatgaaaa aatacagatg ttgaaatctg agaaagtcga
      961 attattttta acacaaaaat tagcagagga tcatattgct aacttagatg ctaaaacttt
     1021 agaggaatat gatcggcagt ttaatcagat acatcacaat atcattttgc ttgttgagca
     1081 ggcaaagcga aggtttgaaa aagcggaata tgatctatta gatcaaggaa ttgtatgctt
     1141 ggataaggga ttgcgagata ttatggtgca gagttaaaaa agcaaaatgt agatagaaat
     1201 gcactaaaac atgaaatctt agaattgata atgcaacgaa tttgaaggga tagaaagcaa
     1261 atgtcacaaa acattagtgc tgtaaaacaa ttaatagaag aagatttaga gaccacagtt
     1321 cagagattag cggaacgaat ccattttgca aattatcaaa ccatctctaa ctggtcaagc
     1381 aggaacagaa atccggaaga aattccaatg aaattttttt atcgaatcgg aaaagaatat
     1441 ggtgttgatc cgataaaaat tttcgagaag accatgattt attactatca tggaacacct
     1501 aacgaagtgt tagaacatct tttggaagag gcaaaagaaa aatcatcaat attagagcta
     1561 tcgcaaaatg acaaagttat tcaagtgaaa tgcgttgatc caacaggaaa gattattgtc
     1621 ggttttgata ttgaaagaaa atccaaaata gattttatta cacaattaaa aaagataaat
     1681 aatttagata ttctaacgct tagaccagat catgaacagt tgtttatgag ttttaaaaaa
     1741 caattgaaag aaatacaaaa aagatataac aaggtgtttc ctaatgcgtg aaaagctcaa
     1801 agaaatcaga gttttttgga taattaacaa agaatgtatc atcttatata ctcttggatt
     1861 tcttagcatg atcttgtttt ggtggttata tttaaaacag taattggaga gggtcaagag
     1921 gatttttatc aagataagga tgtggaaact gcatgtggaa taatagatta aaaacaggat
     1981 tgttattaat agttatttcc tgcgctatga tgattggtat gaggatacaa agggaacaat
     2041 cctattttga agtttctgct aacaacgtaa tagaaaaatg ctattacggt caacattatt
     2101 ggagtgagga agtcagagaa aatatagatc gtgaatatgt tcaaagaatt gtttgggatg
     2161 catattctat caaagattat cctaaaagtt taacgagtag attattttac agtgaaaaag
     2221 ataatcaaaa actctcagat ttgatgatga aaaaagtatg actattatta cgaaagagaa
     2281 acagagtgct atattcgagg ggaaaaagcc tacaacaaaa gtgactattg gggtgacaag
     2341 gcgaagcgat taactgcaaa ttttagagtg tatgaatcag agtttataaa cttgaccttt
     2401 cttaatagtg tttttattga ttatgtaatt actacgaaac gcattggaag ttattcaagt
     2461 acctacgcat ccttagttcc aatgttaaac tcgatgagcc gtttcttaaa agaaagagaa
     2521 aagaaagagc tacagtatat caaagaaatc atgccggatt ttgaaatgca cgaatggcaa
     2581 attgctttat cagattggaa attggcgaat aaggttcgta gaataacacc ttttcaagcc
     2641 aagcgctttt tgagagctat gaaatttgtg caataaaaaa agccaccaga caagttgatg
     2701 gagactaaca caattaccaa cagttgtaat tgtgttagct tacaaattaa ttatggtgtt
     2761 tagtcaatcc caaattgtga aatggcacca ataattggaa aggaaaaatg aaatgtctga
     2821 agatttacga gagctgaagt tacgaaagaa gcagtatgat cagtattttt ccggtttgtt
     2881 gaataaacat attgaagaag gacttgatca tgattttgga aaagatgaat attgtttata
     2941 ttgcgaacgt tacaatgaaa aaatgtctga atatctacag ctgaaaaaag agatacagaa
     3001 attagagaaa aaagtacatg cttggaagta taaagattcc atgtctggtt atgttgaatc
     3061 tcaaaataag ccaagaaaaa agaagcaagc gtatgttcta aagatggtaa atggggaaga
     3121 gttaattgta ttagcgtaca gtgaagaaga ggttcgagaa ctcgttgaaa cacctgtcag
     3181 gaaaaaggtt gaaacaataa atgagataga cgacagccaa tacaatagca tctatattga
     3241 aacaagtcat ggtggatata agaaattagc aactttcctt agaagttcta aagttgggat
     3301 aatttctaaa aaagacataa gtgctgttac cgatagtttt tgtactttat ctgttcctaa
     3361 agcttatttc caatacgata gtaaaagatt tgaacgctta gagaaattct gtagaccgta
     3421 tactgtattg aaaactaaaa aatttaataa aactaaaaga gtttctatac agttaccgac
     3481 tggtcaaatc tcattaggtg aaaaagacta tctgataaaa gtaggaaaag agttaattat
     3541 aaaagtaccg gaacacttat ttaaagaaaa ttttgagata cgtgaaacct tggtatggaa
     3601 agaagaaaaa tagtttggaa gacatagaat ggtataaggg gaaattgaaa tggcagtaaa
     3661 taaaatattg caggtactaa tcatagacga atttggcgga ggagaatggt ttgatgtgca
     3721 gtccatagac tttgaagaag aagtcgtagt tatagaaaat atttacggcg aagtatattc
     3781 atattcgctt gaaggtgaga atattgagga tttacggttg attggtgagt agaaaaaaac
     3841 agaaatatca aaatggagat aaaaattaat agcatgtaaa gatagtcatg cttgggttaa
     3901 aaatgttaaa attcaaaaaa atgagtcaga agggataaaa gtatgaaaag agaggaatac
     3961 aaacaaagat taaacgaact gcttgaagag gacgaaacat taactcatgg ttcgcctgat
     4021 gaaattctat acatgattga taatatggtg atctttggtg gatatgaact tggtaatagg
     4081 tcagttgatc ataatatttt agaatttgat gatgtatcat gggaagaaat tcttgattgg
     4141 ggaattttgg cggtacctga aactaaaacc tatattagtg acactatggt tccttttttt
     4201 gaggaattgg attataaaag acttcccaaa aatgaaaatc atatactggg aggaaactaa
     4261 caatgttaag tgacttattt attttactga tctatctagg tgtaagtgaa attattgcgt
     4321 attttttgtt tgtcattgct aattatggta tttccagtgc aatcgttcaa atgtttagat
     4381 taccagtaag tgaactaaaa aaatgatgag attatcagca tatcttttaa tgtttttgaa
     4441 tgtggtcacg ttaatctttg tagtaatcgg tgtgactttc gatttaatgg aatagtggga
     4501 aatctataat ggaaaaaaga gacacgaatg aacatatttt ttgaatcaat attgttgatg
     4561 gtttttgcga tcagcgctta ccagaaatct aaaaaagagt aatgattgtt taatcatcac
     4621 tctttttttg tagggaaatt gaaatatgtg gagcttgtat tggtttagtt aggattgctt
     4681 ggctataagg agctagttct ttatcaaaac catcaaagta ggttttcagt agcgatttaa
     4741 ctgatcgctc atcttgtttg atttggataa tttcagcatc caaatcggtt gtgagatcag
     4801 gagcttttga tgcgattacc tgattttttt tatcttccaa ctctcttagg ctgtttaaca
     4861 agttgtaaaa agaatcattt tgattgttca ttccaacgtg agttgcaatt ttaacagaag
     4921 tttcgtccgt attgtcatca aaagaactca ttggaatatc aagtagtaac gaggatgttt
     4981 cttttactga ctttttcttt acagcatttt ttgtttttcc atcttttttg ctctttactc
     5041 gagatgtttt tggagctagt ttaggtaaat cacttgtttt atcagtcatt tattagcttt
     5101 tcctttctat taatttatgt tattatatta tcataataac ataaaaaagt acactttttg
     5161 ggggcgtagt tattgaaaaa atctaagaga aagattgcca cattgcttat gttaatggtg
     5221 caatttacag ggattgcaat gagtgtaatc acaataaatc cgcaagtgat acatgctgat
     5281 gggatagcta cttatgatgc acaatttaac tgtactactg aaggtagtgg gtcaggaaca
     5341 gatagcgatg gaaacgatac tggaagtggc gctgtaacag gtgatgtagc caaaaatatt
     5401 gaagatattt acaaatacat gaatggagaa tatggcttat caggtgctag tattgcaggt
     5461 attttaggta actggaaaca agaatcaggt attgatcctc gagcagtaga agggtattac
     5521 ggagatggac agagagaagt tgacttcatc aaacaattta ctgatggtct gatgggtatt
     5581 ggctatggtc aatggacagc tgggcgacat acagcattag ttgattggtc taagaaaaat
     5641 aatggcggtt cttggtggac tacagaagcc caattggact ttatgttcaa aggtgatggg
     5701 ggattcgtag gaatattaaa agattatgct ttaaatgctt ctgatgatgt cgtagaaaat
     5761 gcagtaactt ttcataaaaa ctgggaaatt tcagctgatt ccgaatcagc tgtaatagct
     5821 gtacgtggtg aaaatgcaaa agaaatctgg aagtacatga aaagcaaggg tatggacggt
     5881 gcaaaagatg aaagtaagat caataaaata tccggaggtt caagtaccgg agatggatcg
     5941 aataattcat caagttcagc atcaggaaca gtagaagatg cctgtgcaac tacggatgac
     6001 gctaattctg gttctggatc aattgggaaa gaaggaaaaa ttaacggtaa aaaaggtcaa
     6061 gtaatagcga atatgactta tgaagaagct ttgaaaaaat atgggaaagc gatcacgcta
     6121 ccaaaattca aagaaccaga ttggtcaact tcacctttta acggaatata tcaaggtcag
     6181 tgtacagagt tgacgtgggc ttatatgagc caattatacg atgggaatca accgactact
     6241 ggaaatggtg gttatgtatg ggaaagctac aaatcggcag gagcaaaaat aacgaacaaa
     6301 ccaatcgtag gttatgggtt tagtgcttcg gatggatggt tatgggctgt tggagatgca
     6361 gggcatactg gtgtagttat agcggtgttt gaagatggat catttctatg tgctaacttt
     6421 aatgttccac caacccaagc tccaacaagg ggagtttcgg taacgttgat tgatggagtg
     6481 gacggatcag ataatgtgca tttctttagc ggtgtaggaa aagctaaatt tgaaatcgaa
     6541 aaataaattg gaatgaagga cgatatagat gaatagtaaa gtgaaagata caattatcat
     6601 ttctttgctt tctggtatgt tattagcact gcttgttgga atcctattct tcaataagca
     6661 gaatcaggac atagaagctg aaaaacacag tgtgcatatt gaagataagc tgacattctt
     6721 taaagaggtt aaaccaaatg aggtggatgg attacggaaa gatttagaaa atgatattca
     6781 agaaatgttg aatggatcag ttcaaaataa gtttgcagat caatcaggtg aagaattttt
     6841 gagaaaacta tttattgtaa caggtaaacc accaattact gataagagta gtgagaaaga
     6901 aaaaatcgag tactataagc cttttacttt taagctaaca aactttggaa ttaagacatc
     6961 tagtagtggg ttgtatgaag cattgaccga ttttgaagtt caatacaatg gaaaaaaagt
     7021 gtatgatacc acattaacca tcacactaaa taatgataag aaagtgactg ggggtgcttt
     7081 ttatgcaaaa tcatgaaatc actactgcta aaaaggcgat cggaatactt tccataattg
     7141 tgatcctttg ttgtgtggta ctatttcaaa agaaaaatgc ttatgataat aatgtcaaag
     7201 aattggaaga ccttaaaagt aaacaaatcg aagtagtcaa gaataaccag aaagtgaaag
     7261 ctatgcagga agctcaatac aaagaattgg gaaatgctga aattaaggaa caaagtagga
     7321 aattctttaa tttattttac aactggaatt catgggaaaa atatacgaag aatatgaagg
     7381 aaatacaaaa agaatttcct cagatagtaa agtctagtcc tgttgatatt tctggcacga
     7441 tggtaggcac tggtaatagt ccaatatcta gctatactgc tgattactat acaacaaatc
     7501 aaaaaggtaa aatggttgaa ttgattacac agacaagaaa tactccaaca acagcaacaa
     7561 caacaatttg gcgtggagta acaagcatag gagaaaatca attgttttat ttggaggatt
     7621 tagatagcta tgtaagagca gagtaaaata aaaaaagagc taacggtacc aacgttagct
     7681 ctttgtgctg acgatgataa caaaacctac cgaaaaagga ttgtttcatc acaaacacaa
     7741 acagaaaaga attgataaaa aacccgaacc tcagaatttt tattaatcaa ttcatttaga
     7801 ttataataaa ttgagctgaa cattacaata cgtatatgtt gtaaaacgta aaaaaagtca
     7861 aaagtaaata ctttcttaaa aagtcaaata aaactattaa agacaatata tatggtttat
     7921 ttgacataag aaaatggtct agatgataat tgtaatatag aattgtagac ttaaataaag
     7981 cataaaatac cttttatgat aaggtatagc tcttaatttt aagcagtttt agatataaga
     8041 ttgaaagtaa agtgggggag aataataatg gcagatcata aattagaaaa gttattagaa
     8101 gaaacgtgga atccaaaaga atttagtgaa ttttttatgg agaattttga aactgactta
     8161 gcagtgattg ttaaagatgc tcttagagag caagggtatc ctgaaactgc aaactatata
     8221 aacattaatt ttactttgta tactgaaaat aaaggaactt gggatttttg ggctacttta
     8281 gcaaataagg agttaagtga taaatcagac acaggaattc gtaacttttt tgaatcaaac
     8341 agagatgatt atatgtatgc taacaatcaa aataaattga actttagagt agagtttgat
     8401 gaaacacctg aagaatttat agaaagacaa ccgcctaaag aaaatgtagc aaaggtattg
     8461 gaagatcgtt ggaattcaga tgaaatagtt tcaacaatta gcgaactagg cggacaatat
     8521 gaaccgctag tagaagcggt tagagaagag ctaagactga ataagtttcc tgatgttcaa
     8581 aatattgatg tttctcaaat tgaaataaac gtcaagatca cgaataaact tgattatggt
     8641 agttgggcag atatagcatt agaaaaatac atctactcaa cgttaaaaga gtttatcgaa
     8701 aatagaatgg atatcatgta tctacagcat ccacagtatt taaactttgg tgttgaaata
     8761 gctacacctc ttgaagaatg gaaaatggag caagggcttg actagccttt gctccatttt
     8821 tttattttac aaatgaatac agttcgtcgt ttttaccaag aatgattgtt tgcccaactt
     8881 tgagattgtc aggtgatcct tcagcggttt gtaaggagaa cgttccattg aacttatcgg
     8941 ataccgttat aggtagcttt aattctgatc cttcctcatc tttgagaatt gctgtttttc
     9001 gttcatcaaa ataaacagtc tttattaaat aatttttcgt agtgatctca gcagatttgt
     9061 ctttttttgt agtagtacca gtttctttag aattagaggt attttctacg actattccat
     9121 ctttggcatt tgagcttgtg tttatttgat tgaatatttt cagcgacaag ataacgctaa
     9181 gaatagtcaa tatagaaaca agtgtaatta atactgataa tttccttttt ttgttcattt
     9241 tttcttaaca tccttttttg ttttgattag ttagatattc cttatctaac aagccttttt
     9301 aggctattaa atgaagataa accccaaaaa aatattgcaa ttatgacagt gcaatggtac
     9361 tattattata caataaattt tgcgaaaggg gtaggtctaa atcgcaaaca aaaagaaatt
     9421 gaacctagac aaatacaact tgtataccgc aagggaagca agtttgattt tgggtaaagg
     9481 tagaaattat gttacaaaca taatcaacta ttatccaaat cgtttgccta aaggttcgtt
     9541 caagaaagtg ggtgacaaaa cctatattat aacggaagaa ggaatagaag cattgcgatc
     9601 acaaacagaa aataaaaaca aaaagaagga atgaatatga agagaaattc gcttggtatg
     9661 gttttattgg catcagctgt acttgcgggg ttatcagtac cagtaacctc acatgctgat
     9721 gaatggactg caaacacacc ggatcaaatc caaattacaa caggacaatc aagttatgcg
     9781 ttcaaaaaag gagatactat ttggtcaact gtagagaagc ttaaaagctt aggttacaag
     9841 actactgttg ctgagtttgc cgaattgaac aatgttgatt tggcaaaagg agaacaacgg
     9901 actatcccga ttggacgaac tttcaacatc agtaaagata cattaacagt taaggaagca
     9961 gatggcgaag tagttagtca aactgttcaa aaagataaac ctgatcctat tgaaaatacg
    10021 gagcaagaga acaaaaaatc cgagactaca aaagaatcgg aaccaacagc aaacacttct
    10081 gataagacag aaacaagtgc ttcaagtgtt gatggagcaa gttcatctaa tacaaataat
    10141 tctacaagtg agaaagaagc atcacaggga acaagtgaaa gtagcactac ggaagctgat
    10201 actggaacat caacaaataa ctcacaatca acatcaaatg atgcaaatga atcaaccagt
    10261 acatcttcta gtgatcaatc acaaacaggt tcgagtgatg aaactgacag ttctacttca
    10321 ccaagtaaac ccgaacaacc aactaatgtt gtgctaggcg ttccatttat tagtcaaggt
    10381 aacacaatgc tttgcgaagg aacatctttg ctagaagcgt tacattataa aggtgttgca
    10441 acaaatcaag atttaatgac ttttgttaaa tcaatgccac tatctcctga caataatcca
    10501 aataatggtt tttctggtga atggagacac aatgtggatg gaacttatca aggtatgaat
    10561 ccggctcctg tagtttcatg gggtgttgct aatggcggaa atgttgtaga tatttctggt
    10621 tcatcggtac aaggattaaa agatgagatt gtaaatggaa atcctgtagt agcgtgggta
    10681 acttatgcct ttgaacctgc tcaacataaa ccaatgtttt ggggcgatgc tatttggaat
    10741 cgacatgtag tagccgttga tggatttaaa gacggttttt accatgttgt cgatcctgtg
    10801 tttggggcaa gttggattaa tgcaggatca tttgaaacag catataacgt atctcaatta
    10861 gcggtttcgg tacgttaata aaagatatcg tttatatact aagtagttat atagtcttta
    10921 tttgtccttg cttaactact tagaaaactt gttgctcatg gtgggtagaa ctaaaagggg
    10981 tgatgctatg aatactagag cgttgaaaca gcttgctttc aacgaaaaaa atgattaata
    11041 ggtgtggtga ttttaactta ggaatgggcg cgaaggtcat taccacaccg acatatcgtg
    11101 gctcattttc ttcgcctaac tggtataaag aaggagtaac gagagatgaa gcatgaaaaa
    11161 acgataattt taaaatcaaa aataatttat tgttttgcga ctttagtaat gatgttatct
    11221 acgcttataa tgcctttttc agcaataaga gctgaagcag atgtcacaaa accgacaagt
    11281 gcaaaattta tagaaactgg cgtaaaaaca gacggttata ttagggttaa tatgccgaat
    11341 catccaaatg aatggatgat ttcaagtcaa tttaaagatt ctcatggaaa tattggatat
    11401 tgtatggatt cagaattgcc atccccaaca ggaagtggag ccggaagctt gaagtacaag
    11461 ggagctggca gtgatgagtt ctatcgtatg tttaaaggtg gttttccatc aaaaacagca
    11521 aaggaactgg gtgcaggaaa tgatacagaa gcatggtatg caacacaatt agtttcatgg
    11581 gttttagccg gaaactttaa agtttcacag attgtatggt cacatccaaa ccataccgcc
    11641 gcagaaacag ctcgtgtaaa aaaagcattt gaaaaaattt atgactatgc taagaatgga
    11701 aaagatacgc ccaacactga gttttctatc acagcttcta agacagctga cgaaggaaaa
    11761 tatcacactt tcacatataa aacagcatca aataagactg gtaatgcgaa gctcactttt
    11821 acatcagcaa aaccagctgg tatgaagatt tatgatgcag atggtaaaga aattactaac
    11881 aacactgtga aattaaattc aagttttact attaaagtac ctgtgactac accaagtggt
    11941 actttgtcat ttaaaggtac tgctaatgtt agcacaacca atccttttac ttttgatgga
    12001 cgaggtgtgt atcaagatgc cgtagttatg atcactactt cagaaacaaa ggactctaaa
    12061 tctttgagtg ctaaatggac aagagctgtt ggtcaggtaa cagttaagaa agttgatcaa
    12121 gatggaaatg ctttaaaagg agcggagttt actctaactg acaataacaa tgctaaatct
    12181 gttaagacaa ccggagatga tgggttagct acgtttgatt tgcaacaagg cttaacatac
    12241 agcttagaag aaacaaaagt tcctgaaggt tatcaaggtt catttaaaaa ggatggtatt
    12301 actgttaata ccgatggaca agaatttagt tacactgcta cgaacacaat taaaaaaggt
    12361 aaagtaaaag ttaaaaaggt tgaccaagat ggaaaaccat tagctaatgc tgaatttacc
    12421 ttgacggatg attcatccca aacacaagca gaaaaaacag atgaaaacgg acttgcaagc
    12481 ttcgatattg tagcaaacaa gacctacagc cttgaagaga ccggagtacc aactggttat
    12541 acaggcgtat tcaagcaaga aggaattacc gttaaagatg atggacaaac ttttgaatat
    12601 acagctacaa acactttgaa aaaaggttta gtaaaagttc ataaagtcga tcaagatgga
    12661 aaacctttgg caaatgcaga gttcacttta accgatgaaa aaggtgcaac acaagtagaa
    12721 aaaacagatg aaaatggact tgcaagcttc aatattgtag caaacaagac gtatagccta
    12781 gaagaaaccg gagtaccagt aggttacaca ggtgcattta agcaagaggg tatcaccgtt
    12841 aaagacgatg ggcaaacttt tgaatataca gctacaaata atttgaaaaa aggtgtagta
    12901 aaagttcata aagtcgatca agatggaaaa cctttagcag atgcagaatt caccttaacg
    12961 gatgaaaaag gtgtaaaaga agttaaaaag acggatgcaa ctggtctggc tacgttcaac
    13021 attattgcga acaaaactta caacttagaa gaaacaaaag taccaacagg ttacacaggc
    13081 gcattcaaac aagaaggaat taccgtcaaa gatgatggac aagtctttga atatacagct
    13141 aaaaacactt tgaaaaaagg tgtgatcaaa gttcataaag tcgatgatca agggaaagca
    13201 ttagcaaacg cagaattcac tttgacggac gacaaaggtg taaaagttgt acaaaaaacg
    13261 gacagaaatg gcttggcaac atttaatatt gttgccaaca aaacgtacaa aatcgaagaa
    13321 acacaaaacc ctaatggtta tacagggaaa tggtctaaag aaggcatcac cgttgctgac
    13381 gatggacaaa cctttgaata tacagtaatt aatcaccttg tacctgaaac agtaagaaca
    13441 ggcggaatcg gtacatcagc cttccttctt gtatttctaa ttgcaggatt gagtatggtg
    13501 ctactgtatt atcgagaaca gcacaaagca aaataaaaaa gtgttaatgg aaaagaagta
    13561 aatatgaaaa aagaaggctg acgtatgaaa tcaaaaaaaa ttttttcaaa tttaatgcta
    13621 tttggtactt tgctaggagt gggattgggc gcaagctcaa tctcttctag ccttggacta
    13681 ggggggcaag tagcatatgc acaacaaaac tataattatc aaggaactgg aacgattcat
    13741 atccataaag taacaaatac taatggggat gggaaccctg tctcagatat tgataacaaa
    13801 gggaaagaaa tagctttacc aaatggtgta aaacctcttg aaggcgttga gttctcttat
    13861 gtgaaactta ctaaagatga agctaaatcc ttgggatgga cggatagtga tactacaaaa
    13921 ggtcaacata ttgatgatgc taaagcacga ttattactaa acaaaaaata cagtgaagta
    13981 agtagaatga agactgattc acaagggaat atagacatac ctatacaagt aaatacagac
    14041 gatttaacag ataacttgta tttgattgtt gaaacaggaa cagtcaaagg tgtagttgaa
    14101 gaagcgtatc ctatgattgt taacgtacct gaactaaacg gatcagatga tcaatacttt
    14161 aattatgatg tacatgtcta tcctaaaaac tatttccaat taaactcagc agagcttgat
    14221 aaaaaagacg aagatggaaa ggctcttgcc ggaactgttt ttgaattgcg aaaagatgat
    14281 ggatctgatc aggttgttgg aacttataaa acagacagca caggtaaatt aactgtggat
    14341 ggtttggaat taggtgacta ttatttccta gaaaccgatt tgggaacaga tgctaataga
    14401 acaacctatt tactagatgg taaaaaattc aaatttagcg ttaaacgtgg ggagactgct
    14461 aaagtagttc ttgatgttac caactacaaa cgacctgtaa ttactaaggt aattgataaa
    14521 gaaaccgttg atactggcga agatgttcaa tatacattga aaccatcaat tcctggtaat
    14581 attggtaatt acgaaaaata tgttgtcaaa gatgttctag gtgcagagtt aacttatgat
    14641 tcatcagaag tgatcgaagg aatgacttta gatactgatt acagtatcga acgtgacgga
    14701 caaacgacaa actatgtctt tactgaatca ggtatggata agcttcagaa acaatttgat
    14761 gcaggggatc gcttctttac aattttgatg aacgctcata ctaatagtaa agctaaaatg
    14821 gatacaccac ttaagaatga tgtttcaacg atctacaaca atggatatga acccgaagac
    14881 gaaacaccag ttgatcacgc ttatacaatc attggtgatc accaattcat taaaacagac
    14941 ggagatacaa gcaaagcatt gagtggagca aaatttgtag tgaagaacaa aattgatccg
    15001 aataaagtta cttacctaaa acaagatcca acaacgaaag ctgttacttg gattgcagat
    15061 aaatctaaag ctactgaatt cgtttccgga tcagacggta agttccaaat tgaaggtttg
    15121 caatatacct atgatgtaga ttttgataaa caaggtaatc caacaatcac agataaaaaa
    15181 atcaatgatt atgctcttga agaagttgtt gctccttcag gatatacacc aactagaaac
    15241 gatgttccat ttgtggtatc gaatgatgat gcttctagcg aagtaacaga catcaagaac
    15301 cttaaaacac ctgaaacagt tcgtacaggt ggaattggaa cagtagtatt tctagtggtt
    15361 gcaggtgtgg caggaacagg gctatatgtt gtctacaaac gagaacaaca caaagcttaa
    15421 tgaaataaaa ttccctagag tgtttttggc tctagggttt ctttttaaaa agtgtgtgaa
    15481 aagtaggtat gagcatgaaa aagaagaata tcaagttgat catcttaaaa attgggatgt
    15541 tgctatgttt aggagtaggt ctatatcctg taattactca ggcttatacc tactaccaaa
    15601 ctcaacaaac actaaactcc tataaggaaa aatatgatga agcttctgaa aaggagaatg
    15661 aagaagtctt caagagatat tcagaagtta ataggaaacg aaaagacaca gaacaaacct
    15721 cagtcacgat tcctgaagat aataacaata ttccgtcaaa tggaaatctt cagaaggata
    15781 aattaggtga attagtgggt gaaattctta taccaaagat taatgtggat ttgccaattt
    15841 atgatggtgt aaacgatttc caactatcag caggagcagg aatattaacc ggaactgatt
    15901 atccaactgg tgggattgga aaccattctg ttatatcagc tcattcaggc attcctaata
    15961 aaaaatattt tacagattta gagttgctca aacttggtga taaatttttt attgatattt
    16021 cagggaaaaa acttgcttat caagtagatc ggataaaaac aattctacca accgaatttg
    16081 aggatttgct tcctgtggca ggaaaagatt atgtaacact tatgacatgt acacctgttg
    16141 gagataattc tcataggtta ctcgttagag gggttagcat accttaccaa aaaagtattg
    16201 ttgagaaaga aaagaagaaa gaagatttaa aggtaaaggc gcagatgaac tggtatccta
    16261 tcgtagttgt aggggtttta ttaataatag ctgtgatagt cattattaaa aaaatagtag
    16321 caaagagacg aggaatgaaa aatgaagaat aaaatactac tttttttgac tcttatttgc
    16381 actttggttt taacaattta tcctgcaaat cagctctatc atcaattcaa aacaaaagag
    16441 aatgtggcgg aaatccaaga gaaaattgag aaaaaacaaa ctccttcaaa agaggaagtg
    16501 gcaaaaaaag tcgaacaaat aaaatcgaac agcggtaacc aaggattaga caatactcag
    16561 aaaacagata aaggagtaga caatactcag gaaacagacg gtggatcatt tgatattcgt
    16621 gaagctgaag gaaaggtcaa tccatacaca ggagatgttt tttcaaaaaa tcaaacgaaa
    16681 gattttgaac catctaccta ttcaggagcg gttggttatg tctatattcc aaaaataaat
    16741 gtgctagagc ttttgtttct aggcgcttca caggagcatt tatatagagg tgtggcacaa
    16801 atgagtgcta ctagtctacc tttatcaggt aaaggtcatc aaacagtaat agcaggtcat
    16861 cgtggcggtt atacgtcacc acaatttctt tatgttgatc aattagtagc cggagaccac
    16921 atttatatag attatcttgg tgaaaaagca gtttatacgg ttttccgaac agacgttatt
    16981 agaaattacg gtcagaattc aacactgact gatattcctg atttagatat gttaacactt
    17041 tatacttgtc atccatatcc tcagaactac gatcgtttat tagttagaag tctaagagat
    17101 gaaaaagcaa aatacatcac gccacaagta acagttggca ataccgcata cgctgatgcc
    17161 attgatagcg agtccgtttc agataagaca gaaactgtta catcttctaa tgatttattt
    17221 gaggatgttg atcgtgccaa caaggaatta gatggaaata atgatcttaa tattccggaa
    17281 caaggtagcg tagagatgca agacagaacg tttgctgaaa aggcaagttc aatatttaca
    17341 ggaattacca ataaacatca gaaaatgctt ttagtcattt ggactgtaat aggagcatta
    17401 gcaagtctaa aggttcttat tcttttaatc aaagagtttc gaaaagcata atgaagatta
    17461 taaaaattga tagagtacag taaaaaggga ttgatactat tattgatctc ttttttattt
    17521 tgaatagagc tagattcact tttaaataaa atcaaaactg atcttaattc taaagaaaaa
    17581 gtcaagaaaa aaatacaaca aattttttta tcagaatatt tatcagaata ttattagaaa
    17641 agataaactc aaaagcttaa tttaataggg ttattggttg tatttagaaa taatgtatgg
    17701 aaaagctata aatcaataga taagatgtag aaaatcttat ttgtgaatga aaaacaagtc
    17761 atcgtaaaat attaatagga ggtgtagtaa tggtgggaat atcaaaaaca gctttagact
    17821 cattagctaa actatttagt agcaggtttg atggtgcaga atttgttgaa tatctaagcc
    17881 aaaacgaaag tggaaaaact ctgatcgaaa caatgaaaaa agactatttg aatgaaaatg
    17941 actttccgac atggacgaat attgaagatg ctacatggga tgtagtatgt acgatatatt
    18001 ctccttgggt aacgattgct gattttgttt tagataaaat tggaggggat acataccctg
    18061 atgccaaatc tcttgctaca agtgacttta aaacgttcta tggttatcta ataggtctaa
    18121 taggcaatgg aatagatttg aaagttacgc caaaacaaac acctgaagaa attgaaaaag
    18181 aaaaaggaga ggttcaaatg gcttatgcaa atgacagaga aaaagtaaaa ttattggaag
    18241 gtaaattatc tgaagaagaa cttggaaaaa tggtgcaaga aatcaatgaa gacagcaact
    18301 attttcctga attgaaggta tatcaaaatg acgaagattt ctttaatgaa atgtttagta
    18361 atagtccatg ggaagcagtg agtaaaacaa atttaggaga atggaacttt caagatgatc
    18421 tagttgcatt tagcaatgat gggtatttac gatcatattc agaaagtgag tatagagctg
    18481 aattaaaaac gtatgaaacg gatattctga tgagctattt aaatctatta gagagtggtc
    18541 agggagagtt aagagataaa gttgaagaac tattaaaaga agagctatag ggattatttc
    18601 aacataaaca caagcagaaa actactaaac aaaacgtacc atgcaaaaag tcggttaaac
    18661 cggctttttg ctctattatg agaggagatc aaaccatgag tgcaacaacc gatatggctt
    18721 tagaagcagt aataaaagat aatacaggaa atgtcgaaaa cgtagaagac tttagcgata
    18781 ttgcaatcaa tctttcagca tggatttcag ctgtaggaga agaaaatatc gaatttgaga
    18841 tcaagaatat ggatgactta aaagcaaaat ttccttttat cgcatcgaaa ctaagtcaag
    18901 ttgccgagga taaatcgtgg aatgaattag ttagtttttc cgaaaaaatt tatgatatta
    18961 cgcaagaaga tgagtgtgtt tcatgggaaa tgaataatca atacaagaaa attgacgctt
    19021 tcgtcaaatc acgtatagaa ggaacagctt ttgagtttga aggtatagat accgcagata
    19081 attggttaaa taaagaatcc tttattaaaa gtatagcgga tgatcttgaa aaacaatggt
    19141 ctaaggaaga ctatgcagag tacctaaaaa cagaaagaga tacagaggtg aaagaaaaac
    19201 tcactgaggt atgtttggat gaacatattg atcccaatat cgtaaaacaa gaagactatg
    19261 aagtggttat tgataaagtt cctactgatt ttgaggaagt tgtaaagtat ctagttgaag
    19321 aagcattatc gcaatactct tcagatgagg aagtggacga agaaactgtt cgtaccagag
    19381 tttggcaact ggtttttgat gacaaagatt ataaatattt gagtgatcaa aactcggtta
    19441 attggagtgt acgattatta attgatcaaa atgatattga ataaaataga gctgaaaagc
    19501 tctatttttt ttgttaaaaa aactagctat tttattaaaa ataatttggt tcaaatttta
    19561 tttttttaac ttgataattt cttgccatcc ttttttaaat tggctatata tcaaggttta
    19621 tagtgggaat aaataaaaat acatttgaca aaatttacaa tatagtttat tatatattta
    19681 tcttattatg ataacacaat aaaaggaatg gtggtatgaa aaagaagaag tttagtcgat
    19741 cgttgttagg aattttggtt ttagttgcgg ttggtcttag tggcaagggt tactttcaga
    19801 ataacagcac tcaggaacat gattttcaga tcaaaagtag tgttcagata ggtaataaag
    19861 cagacagtga atcaaaagaa tttttaggga tacgttatga tccaaacaaa tatccaaata
    19921 atatcgtaaa cattaatgat gggaaatata cactgacaaa agaagaccat gaagcattat
    19981 cagaaaaagg cagtcaaacc ttttgggtaa attatgcaga tttagatcaa cttggaagag
    20041 caggagatgt tgaagcgttg atcacctatg atgctgtaaa ttctcactca tctaaaaatg
    20101 tggaacgtcc atctttcaaa agttctgtcc atattgcagg agaatacaag gatggagtat
    20161 ttgatcaaga gagagatact tgggtcggca aagattcaaa caatgaaatc actcagctag
    20221 aaggctataa aggttatctc tataacaaaa gtcattcatt agcatggtcg ctaggaggag
    20281 atatggaggt acacaattta acacttggaa cgagagcgca aaatgttggg aaaaatgatc
    20341 agaatggagg gatggcttac tcagaaacga ttgtacgtga tgcaatctat aaaaataaga
    20401 atgaaaaagt attgtataaa gttgatccaa tttataaagg gaatgaatta gttcctcgtg
    20461 gttctcatgt cgaagctttc tcagttaacg ataatggaaa aacggtaaac cttaatgttt
    20521 atgtatttaa caatcaaaaa ggaatcaaca tcaactatca aaatggtact tgggtaagag
    20581 attagaaatt gagaaggtga agaattggaa acaataagca tcaatggatt agataaatca
    20641 gttgcaaatc gaataaaaaa aggaaatggc tactatcttc tgaagaaggg taaatattca
    20701 gttgctagag agttagaaac aatgagtatc gtgtatgtag ctaagaatca ttttgacgta
    20761 gaacaggcta tttttgatcc agattctgaa atagggaaat gtgttgaatt atcagagaaa
    20821 actgttagta caaaaggggc gatggtcatt gagagagaaa tgttagacga aatcagagta
    20881 atcattccta aagatacgca gatgtgtgag gacagcataa gtgacgatga accaaaatca
    20941 gaaagaaaat aaggagatat tcagatgagc gaaaaatcca ttgaattaaa ggaatataag
    21001 cgaagcgatg gagaaaaata ttatcctgta gccagttttt ctaaatcagt atatctggtt
    21061 ggacgtgcat tggtagttgg tgcaattggc atatatattt tgcattgggt aacaaaggtt
    21121 gtacaactgc ttgtagatac cttagtaagt attcttgtct cgttaaatga tgttaacgga
    21181 acagatgatt taggattaaa agagatgttt aaagagttac tcaatgcgtt ttttcccaat
    21241 tatttaagtt attcatataa tgtacaaagc ttttttgcaa agctacactt ggtagacatc
    21301 tctattttag tattagaagc aggaacggta ttctttgttg gatatttggt tactttgatg
    21361 ttcaggcatc actatggaga acaaagacca ttttttgatg atatggaaag tagaaggttg
    21421 aaaagagaag ctctagaagc tatggatgtg aagtatgatc caaacaagat tataggagat
    21481 gaaacgaaca acaaaaaaag aaaacgaaac aaaacgcaat tagaatccga tgtgctatca
    21541 acgcttaaaa atatcaaaat ttctgttcat acaagacgtg atctaagtgt caatgaagat
    21601 attcgagaat atatcgtaaa aatcccgcaa ccaaaaggga agaaagttag agaaacagtt
    21661 ttacgacaaa ttcaagattt agggtcgatc ctgacaagag caacaagcgg acgagccaaa
    21721 tttggtgaaa aagaagatga agtttcaggc gattatttca ttttccgagc atcagtaaat
    21781 attgaaagag aacgtaagga aagagtaagt accaaaaaca aagaaacgga aagagcaaca
    21841 tcttcaatcg cagtttcaaa ttcacaattt tcttttccaa tcaatctctt tgccgataat
    21901 tccaaaaata tccaaaacca aaccgaaaaa gctagaaaat atgccgaaac tcaaaagcaa
    21961 tctttagaac tttatctatc ttctgctgac gtacctgcaa gtctaacaga tatgcacgtt
    22021 gggaatacat ctattgaata tttgtttact ttatctcgta ccagaaaaat tcaactaagc
    22081 tcaatgaaag aagaaattga aacttacttg catatatcag gtgtaatcgt gcggatcaac
    22141 gcagggcgta ttcaaattac tttaccatta ggagaaaatg atcgtattcc aattgatgta
    22201 ccatcaatga tcaatagcgt gttttagaag acatcacaaa tagaaacaga aaatacaaac
    22261 taccagttca caaacagaaa agtaccgaga aaagtgagga atagaaatgg ctatgaaatt
    22321 attagagaaa gtcaaaaaga gagccgaaaa agctgaatct attttgctag ataaagaacc
    22381 taatataaag cttaatggaa aattcagtgt caatatgttg aacacaaagg ctaaagaccc
    22441 aacagatgct atttttggaa ttaagcctga tggcacaatt gctcattttt gcatcagcga
    22501 aatgccacac atgttggttg caggaacgac aggatcaggg aaatcagtat tactgaatga
    22561 aattcttgtt acagtaatgt gtcactcaac gcctgatgaa ttaaaaatag gaattgtaga
    22621 cccaaaaagg gttgaatttg gaagatacaa aaaattgccg ttcatgttgg ctgatccaat
    22681 tacagatatg gatgaagctt atgatttttt tgaatatctt gtgatcttga tgcatgaacg
    22741 ttacaaactg atggaaaaag cagggttaca aaacatcacg ctatataacg aatatgccga
    22801 aaaaaatgga ttagatagat ttccctatgt gatcttatta gttgatgaat attcacagtt
    22861 ggttggaaca cataaagaag tagaaggttt gattgttcaa ttaggacaaa tggcaagggc
    22921 agcaggtatt catgttatct tagcaacaca atctcctcga tcaacagttg tcactggtat
    22981 tatcaaagcc aattttccgt cacgtgtttg ccttatggta gcaagtgatt tagaaagtag
    23041 gattgtatta gatgaaggcg gtggagaaag tctaagtcca aagggagata tgcttattaa
    23101 gcttgttgat ggaagtatgg ttagagcgca gggagcttac atcagtaaca aagaaattga
    23161 aacaatcttc aatcacctta gaaatactat gccagaacca gaatatgttg attaccgagc
    23221 aatcgtagct gaaactcgtg gtgagggcgg tgaagacgag gattttggtg gaattggtaa
    23281 agctcctgta agaaaatctg ctaaaagaaa tttgaacaca cctttagcaa atccatcatc
    23341 taaagagcca tcaattgaaa agaagagtgc gtttgctatt aagcttgaaa aaaataaaga
    23401 agaaaaagct aatcagggag ctaagtcact agaaaaactt cagcaagaaa aattagaaaa
    23461 actagaaaaa gcattatcat catcaacttc tgaaactcaa tcttcaaagg gtatagataa
    23521 tggacaatca attacaacta aaaacaagcc taaaaatagt aattcaaatc tctcggtaaa
    23581 tgttgttatc acaactgata aatctgaaaa aaaacaagtg aacggaacta cttcacccat
    23641 catgcaaaaa atgataaagg caaaaaaaca ggtgcagaaa tgattatcat tagttccttt
    23701 ttgagatggc taacaccata taaatataca gatgaaatag ttgagaagcg agcgaagaaa
    23761 aaagttaaaa aaacgatgtt aactcctaaa gaaatgctag atcgtggaat attgggagtg
    23821 aaggttttag aagtaaacca accgtttgaa ttggataatg cgagagatag gttgcttaga
    23881 gctgaagttg atgtcgcata cttttatcat gataaaagca agcatcaaat agctatcaaa
    23941 ttcaatccaa aatcggcatg gcgataccaa gcaagagatg acctaaagcg taggaagctg
    24001 aaaccgatgc tctatcctga aggaaaagag gcatttgata gaacccatat tgttcccatt
    24061 ggatttcatg gtacagagtc agataaccgc ctattggtag ggtggaacag ctcacaaaat
    24121 agagttcaga tcaataaatt tgagaagaag gtaagaaaat ttaacgatcg gaaaataata
    24181 ctttggtttg ccaatattca gaagcaacca gatgggacag caaagtggga agcggtcata
    24241 tttgatgaag atggcaaaaa agtaatgtct gaagtatggc atgataaagc taaatttgtt
    24301 tggcagtaga ggtgaaaatt gtgttaacta acctaaaact atatagagag ggatttaacg
    24361 aaaaagtaag tatcggtgaa tctattgaat atttaggaaa gaagtacatt attattcgta
    24421 ttcttgaaat taaaaaactt tattttagta aatataataa tttagcagtt agagttctcg
    24481 tccaaaaggt tggaaatcca agtgactata gaagtgatta ccataactat aaaacagaat
    24541 catctgtaat taagcgttat aatcaatcaa aaaatgatga gagaatattc aaagtaggag
    24601 aaattattat ttctaagtat gggatagcat acgaaatagt ttctattaaa aaattttatt
    24661 atgaatttgt tgatttggtt gtagaatatt ctgttcgatt gatcgttccg tggagtaaag
    24721 aagaaattaa tgaggcatta aaacatgaac gaaaaagtac attcaaagtt ttagaaggtg
    24781 aaaaaaagtg aaaacagacc ttactaggca agctgagaag tgcttatggc atgaaacaaa
    24841 tcgcatgggt gtatttggtt gtttcgaagt cactatcggt tgggatggca gtgaaagagt
    24901 agattatata acttattcag tagataacac agttcgatgt tatgagatca aagttacttt
    24961 agctgattta aaaagcaagg cgaaacagac ttttatcggt gattataact atttagttgt
    25021 tactagtgat ttgtgggaga aaatgcaaca agacgataaa cttatgcgga aatattacta
    25081 tcaaggcgta tacgtgttca atgaaaaata tcctagcata ataagtgtta agaaagcaag
    25141 gaaacaaaaa gtatcgctag gtacaagatc aactgtatta gaaagcatgg ttcgttctat
    25201 gaatcgtgaa gttaagaaac tctataaagt aaaaccattt tgggagatag cagaggtgga
    25261 aaaaggatga ttcaaacatt tgcaaaaatg agtagaagtg cttggattga aaagctggaa
    25321 aatgatttga gtgttatgcg attagctcca aagaaattca aacgagagat tgcagaaaaa
    25381 gaagcaattt tgtcggtatt gaatgcggta gaactggacg aaccgaaaaa gtcagttgat
    25441 gtacctgaaa atgtagcaga gttactagat tattatcgta attcgtcaga tgtcgatttg
    25501 ttagctctga tattaacatt tcacgattgg tattactgtc aaaatgaaga tactcagcat
    25561 gataaacatg ataaagcagt tgactggtta atagagaatc cagaaaagtt catgcgtgct
    25621 tggctagatg gctatgatag caagaaaaag ccactggaaa gtaatgagga aacgtattta
    25681 acggcagaac aatatgacta tgctagaaaa acaggagatt gggaatctgg taagactttc
    25741 aatcacagaa aagatcaacc aaaaattagt ttttcagata tagccgcaat tatcgaaggt
    25801 atctactatg gacaagccga tgaagagatc ttttctatac aagatttatt agaatttttg
    25861 aacaaaaata aggatagctt tgtagaacta actgattatg aggaggatac agcaaatgga
    25921 aaattatgat ccaaatatgc gatggggtac acatacgctt aaagtttctt ttcaacgttg
    25981 ggattacaag ggatttgtga cttttagaaa agctggaaat tgtaaaggat tagatgtctt
    26041 agcgttggat gaagactatc tatatgacca tccattgacg gataatccaa tcggatttgg
    26101 attgcttcct gaagatgatg agggcaatga atggttcaaa atgattctta caaacgataa
    26161 aggtgatcaa ttatttgtag aagatacttg gtcatatttg agtgaatata ttgttagcat
    26221 taaaatcatt gattttgtag cagataaaga aaaagagatt ggagaaggta aatctaatta
    26281 ctagctaatg aatagcaact ttggaggata agtcaaaaga ataaaaaaag aatactgtgt
    26341 gtcattgcag ggatgtatct ttgcatgata gtatctgcgg ttaccattta ttggttagga
    26401 aatcagcaaa acagtttaat tgttgatctg ggggagttaa aggttggatt acgaatgact
    26461 agatggcttt tattagctca aacagttggt ttagccatat tgacttggat atgtgtggaa
    26521 aaaataacat ctaataataa tcaagagatt gttgaatcaa caaacctaag aataagaata
    26581 actgaacaag caattgaaaa gcaaaaagac aaagaaaaaa aggaaataat gtcaatttat
    26641 ttatctagtg gagcatcatt ttttcatgaa atagattcga tgaaactgag tagtcaagaa
    26701 atcattgata gatggaaaaa catgcctcta ggtggtgagt taatcgttga agatagtgat
    26761 ggttggtcag cttataagaa ggacgagatt gtagctatca cgattttgtt tgaatagtaa
    26821 tatattacca ctagataatc tgaatatgtt gatctgctag tgacgtacca aatgcgttta
    26881 atagaacctt ggtcacaggc agaaattgat aaagcagtaa aattgaaccg attatcaaaa
    26941 tttaacgtat taggaaatgc cttttaaaaa tcgcaataca caaacagaaa aataccaaaa
    27001 aattagcaat gaacgtaggt aagaacatga caataaataa taataaccct aacaaatata
    27061 atgctcttgt ccaatgctat atcgaaaaca aaattacaat gcaagaagtc gctgatgaat
    27121 taggcaaatc aaaaagccta gtatcacacg actttaagca caatgttttt ccgaacccga
    27181 ttgagctgag gaaaaaattt caaaagagag tttatcaaga aatagggaag tggattgatg
    27241 aagggaatgc aattgaccct ttaattgaaa aatatccttt tttaaaaagt aaaaataaaa
    27301 ttcaaaggtt aattcgtcaa ggcgatattc aagctaaaga acctgttttg acaaccaatg
    27361 atttggctaa aataaatctt cataaacaga ttattgaaga ctttgaaaaa aatctttcaa
    27421 caactgaaat tttaaaaaaa tacaatattc cgagaggaaa gctaacatcg actcttttta
    27481 gagaatatgg agaatcggca gttactaaag caaaggaagt agcgaatgag agaaaaaaac
    27541 aaaaatagta gtcgatacct agtagcgact gtagtttttg tttgtgtatt gattttcctg
    27601 tttgattttt tgcagattgg aaaaagcata attggaaata cattgagtgc aaaggaaatg
    27661 gttgctttag gtggtttgta tgaaggacat ttgttcccga atatattcat ttctatcgtt
    27721 tctcatatta acttgtacca tttaatttct aatttattgt tcttagtagt tcttgaagaa
    27781 gcactgaatg atcaatatga gtggttaaca ctattgattg ttttaatcat atcaggactt
    27841 ctaggaagct tagttccttt tctgttacaa agcaacatcg taagtatggg gcttagtgga
    27901 ggtatttttg gatggattgc tttatttgcg attgcataca tccgctttga tagtaaggaa
    27961 catttattac taagtgtttt tatattattg ataatcggca ttttactggt atctactttt
    28021 gaatcaggcg agacaaacgt cttgtcacat gtaatcggat ttgtctgtgg gatgatcttg
    28081 ggatttattc taaagaacat ttcattgatc aagcaaaaga attaaaaggg agtgctaaaa
    28141 atgccaaaaa aaaaagaaaa gaaaattcca gtgtttttgt ctgtctcaga aatgttatca
    28201 cttcaaatac tgttaagaaa aaagattgaa acaatagacg aagaagttag aaataaaagg
    28261 gctatgtacg aacaacgtga agcgctaaga gaacgaatcc actattttga tgatatacac
    28321 actaaaatct atgaagcacg tacaaataac gaagacgaaa gcaaattagg attagctcaa
    28381 caacgaatat ttgaatggct aaatacgaat actgagcaag aaccgttaag agcaattagt
    28441 aatctttttg aatttggaac gaagcattgg tttaacgcag tagaaaagga tgctgactta
    28501 caagaagcct ttgacacgct aagtaaggat agcaaatcca tgcgagattt attgattact
    28561 tatctaaatg agcatgagtt tgaacaaacg aaaaccaaaa agaaaaataa agaatagaaa
    28621 gttgtgatga atcatgctat taaccctgtt aggttattta tgtttaattc ttgctttttt
    28681 tggggcaatc gctataatta ttggtcaatt tcaaacaaat aatccgatta tgcgtaacat
    28741 aggcattgga attgtgattg taacctttat tgtgtttaat gccactaaga tttttggaag
    28801 taacgataaa caatttggta tatcgggagt taataatagt tatgctccag attctttggg
    28861 aagggtagta atagatggaa catttgaaaa tgtaacgagt tataaaaatg tttcgctgac
    28921 agtgaaggaa gatggcaaga aagcatcaga cgacgttctc gctactatta agccgtctca
    28981 atcacaattt gaagtgtttt atacagttaa agagaatagc cctgtacaca agctgacatt
    29041 ggtttttaac gttgatggag aagaatacta taaagatgta aaaataaata aaacatcaac
    29101 gaaaaagatt atcgaatcag cgaaaaaaga agtagctaca gaagaaagta caactgaatc
    29161 aagttctact gtcgaagcgg aactaccaga taaaattgat tttgatcctg aaaaagtcaa
    29221 atcgtttgaa tctgtttctg gttctatggc tgatcggttt aacactgaag tcttcaaagc
    29281 tatcaaaact gttaaattgg atactgaaaa gcaaatgatt aatgttacct tattcaattc
    29341 agttgaatct atggatcagg ataaaaaagt tacggttgca agagaactag gaaaaagagt
    29401 aattggtttg tatcgtggta ctgctaaacc agattcagac ggtgcagatt tgccagttca
    29461 tgtagtttat gaagacggta aagtttttgg agattctaca gcaaacgaag cattaacgat
    29521 taaattggct aaatagccag aaagttataa taaacaaatg gtgtcacacc aataatatat
    29581 aggtgtgaca ccttggagat gatagctgat ggtagtaaat aaagaaaaag ttagaaagta
    29641 tgtgacttta aaaatagctt ctgttaaata tttagaaaag cgagctaaag aagaagaaag
    29701 agcaagaggt aagaaagtaa cagtttcaga gttgttagaa gaattgatcg agaatgatca
    29761 aacaataaga cgtgcattta ctaataggtc tatcagataa caatataatt aataaaaaag
    29821 aaagagtgat ttggtcttag actaaatcac tctttttata taaagtttct ttacactcat
    29881 tccgaataat gttaaaattc tttatatgat atatagttcc ttgtcagagg aagagataac
    29941 cagcaatatt ggagggataa taagtggaaa acaataatga tattagacat tttagcattg
    30001 gggaaagagt tacaacagta agtgaattat caaaggatta tagagataaa gagggaacga
    30061 ttatttctct taataagaat cgaagaggcg tttggcaata tgttgttgat ttaggtgatg
    30121 gcgttacagt tgagtgtaaa gatagtcaac tgatcagaaa taatggcagt cattccattg
    30181 aaaaagcaat tcttgtatac ccaaaagtat caagtaagat catgaatgac tggttgcttg
    30241 gaaaatttgg tgagtacaat aaaaaagaaa tcgcagaagt gatcggcaaa tcttatccaa
    30301 cagtattagg gtatctaaaa aagataacga gtaaagaagc aaaaattgag gatgttttaa
    30361 cagtaagaga gcttcaatca ttaatcgagt atcggaataa acaaataaca aatcctgagt
    30421 accttagaaa agaagatggt ctattgaaac aattggaaca agtaaataaa atccggaaga
    30481 tgcaaaaggt agctccttta agccttgata cctttaaaaa aatctatttg aaagagatca
    30541 aaaaagacta atgtggactg taactttacc gttccaacct gtatctgcac caagaccaag
    30601 tgcaagaagg aatgctgctg atgaaactaa tgacgtagca gagaaaagga tttcaaccta
    30661 ctatgataaa aagtatcttg attatttgaa aaaaataaag agctttatcg aagatcaaga
    30721 tttattagat gatgaatttt actcgattgt aaatgatcct atgggcgcaa ttatggaagt
    30781 ggacttttac taccagatac caaaaagcta taaaaaagtc tataacatta tgaagacaac
    30841 agcgccagat ttagataatt tagtaaaaag tgctatggat ggtattttta acatctcagc
    30901 tatacgagat agccatatca caggcttaat tgcttttaaa tataatgtgg cgaacgaagg
    30961 aaaaacagtt gtgaggatca aacgatattc agaatttgag tgggatactt ctgaaggatt
    31021 gaacggagat atatgggaag ctacatttga ttttaaacca gtagcaacgc caagaccaaa
    31081 gtctaaattt agaggaaatg ggattatcac ttactatcca aaagagtaca acgactattt
    31141 ggaaaatatc aagaatacgc ttaaagagaa agatttggta aatgatcaac tgaaaaaggt
    31201 aatgaatgct cctatggggg ttatagctca gatagattat ttttaccaag tgagaaaaga
    31261 taagaaaaag cttgattcac tgatgcgtac ttcacagccg gatattgata atttagtcaa
    31321 aggaacattg gacggaattt ttaacaagga aataggcatc aaagatagca gggttgtagg
    31381 gttaattgct tttaaataca atacttttga aaagtcgaaa acaaacgtga gactacagga
    31441 gatgggctga tgaataataa tgaaaaaatt cttcatgtac ttgatagctt tgaaataatt
    31501 caagaagaac taaaaaaata tcgggatgtt ttggaacaaa gatatgattt tgtcaatcag
    31561 caaaagagta atcacatgga tttcattctc aatatgaatg acttgaagaa gaaattagtt
    31621 gaacgaaaag aacaagaaaa gttgatcaaa gcttactttg aactcggtga aaaagaagtt
    31681 aaaaatgcaa tggaactgaa tgaagagcgc agggttttag atcagctgtt agaacaatta
    31741 ttggtgatgt ttcaaaaagg acgaatagat gaggatttaa ttgaagaagg attgagaaaa
    31801 tatccttcaa actcaggtat cggaatagtg ctgaaagcga ttgatgaaga agaaattgaa
    31861 gctttcatac cgccagagga ttttgaatcg gcaatggaat atataaaata ttatagccaa
    31921 gggatcactg cttttagaga gtttgatcct gaagatgtta ttcatgactt gaacaaccta
    31981 aaagaatggt gtgaaagtta tggagtcgat gattccggat tggactactt gatcagtatt
    32041 atggaaatcg aagaagagat gccggataag cctgatccaa cagatatttt agaattgatc
    32101 catgaagcta gaaatccaat tgcctatatt tctagaggat atactgtatt ggaatactat
    32161 aagccttata tatcagcgat gaaccattta agacgtgtat taagggaaaa acgtgaatac
    32221 cgctcagtgt tgaatgcaac gaatagatta gaaaaagctg tttcagagct agatgcttat
    32281 tatcgtgaac attacctgca agcaggtggt atgccaagaa atacgaaagc aaatatcagt
    32341 aggtatataa aaaaagctga gtaggcggag ttagcccata aaatggcaaa aggtaacctg
    32401 aaaggggcat tttgaatgaa aagggatgtt tttgaattgt acgtattaga acgtctagaa
    32461 gacggcatga tggcacaaga gataattgat gaaattgatg atatgatcag cagatttggt
    32521 caattggagt tgaatgatgg aaacaaaaat tcggtagtgc taaaacaatt ttggttgaac
    32581 aatttttcgt ataaggattt tatcaaagaa aaggcaaata cattgtactg actaaattca
    32641 cgaggagcag tgatgagagc ttattgctaa gagcagttag gctctttttt gtttgtgtaa
    32701 aattatttgt tttataatac acatttatac acaaaaaaat attgacagat agtacacaaa
    32761 tgtggtagga tatatttgta agcaagttag attaacaagt taaaggaaga gcagttatga
    32821 aaaaagaaga actaatagat atatttcaaa ttgttgaacg agcaaataat atgggaatta
    32881 tgttttttga tcgtatttct ttaaagatg
//
LOCUS       DAFCNS010000030        32533 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFCNS010000030 DAFCNS010000000
VERSION     DAFCNS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.030"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="LNE23_001291"
     CDS             131..505
                     /locus_tag="LNE23_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK6931156.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LNE23_001292"
     CDS             complement(619..1248)
                     /locus_tag="LNE23_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6931157.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LNE23_001293"
     tRNA            complement(1382..1454)
                     /locus_tag="LNE23_001293"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="LNE23_001294"
     tRNA            1668..1741
                     /locus_tag="LNE23_001294"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="LNE23_001295"
     CDS             complement(1879..2043)
                     /locus_tag="LNE23_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931158.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="LNE23_001296"
     CDS             2338..3666
                     /locus_tag="LNE23_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBK6931159.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="LNE23_001297"
     CDS             3868..4701
                     /locus_tag="LNE23_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBK6931160.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="LNE23_001298"
     CDS             4717..5454
                     /locus_tag="LNE23_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBK6931161.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="LNE23_001299"
     CDS             complement(5609..6577)
                     /locus_tag="LNE23_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBK6931162.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="LNE23_001300"
     CDS             6809..7642
                     /locus_tag="LNE23_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBK6931163.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="LNE23_001301"
     CDS             7676..8515
                     /locus_tag="LNE23_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6931164.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="LNE23_001302"
     CDS             8541..9101
                     /locus_tag="LNE23_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK6931165.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="LNE23_001303"
     CDS             9300..11021
                     /locus_tag="LNE23_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBK6931166.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="LNE23_001304"
     CDS             11186..12328
                     /locus_tag="LNE23_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK6931167.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="LNE23_001305"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="LNE23_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBK6931168.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="LNE23_001306"
     CDS             14128..14775
                     /locus_tag="LNE23_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK6931169.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="LNE23_001307"
     CDS             15168..17813
                     /locus_tag="LNE23_001307"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBK6931170.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="LNE23_001308"
     CDS             18123..19436
                     /locus_tag="LNE23_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBK6931171.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="LNE23_001309"
     CDS             19426..20079
                     /locus_tag="LNE23_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6931172.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /gene="radC"
                     /locus_tag="LNE23_001310"
     CDS             20134..20826
                     /gene="radC"
                     /locus_tag="LNE23_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBK6931173.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="LNE23_001311"
     CDS             20976..21176
                     /locus_tag="LNE23_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6931174.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="LNE23_001312"
     CDS             complement(21430..23109)
                     /locus_tag="LNE23_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK6931175.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="LNE23_001313"
     CDS             complement(23111..23323)
                     /locus_tag="LNE23_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBK6931176.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="LNE23_001314"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="LNE23_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBK6931177.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="LNE23_001315"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="LNE23_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBK6931178.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="LNE23_001316"
     CDS             27298..27495
                     /locus_tag="LNE23_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931179.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27648..28133
                     /locus_tag="LNE23_001317"
     CDS             27648..28133
                     /locus_tag="LNE23_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBK6931180.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="LNE23_001318"
     CDS             complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="LNE23_001318"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBK6931181.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29010..29537
                     /locus_tag="LNE23_001319"
     CDS             29010..29537
                     /locus_tag="LNE23_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931182.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29628..30365
                     /locus_tag="LNE23_001320"
     CDS             29628..30365
                     /locus_tag="LNE23_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK6931183.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30369..31286
                     /gene="miaA"
                     /locus_tag="LNE23_001321"
     CDS             30369..31286
                     /gene="miaA"
                     /locus_tag="LNE23_001321"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBK6931184.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31288..32517
                     /gene="hflX"
                     /locus_tag="LNE23_001322"
     CDS             31288..32517
                     /gene="hflX"
                     /locus_tag="LNE23_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBK6931185.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10772 a         5522 c         6874 g         9365 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt
    27601 attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta
    27661 tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt
    27721 atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc
    27781 aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga
    27841 cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga
    27901 tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat
    27961 cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta
    28021 aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt
    28081 atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag
    28141 catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta
    28201 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
    28261 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
    28321 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
    28381 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
    28441 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
    28501 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
    28561 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
    28621 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
    28681 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
    28741 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
    28801 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
    28861 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
    28921 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
    28981 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
    29041 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
    29101 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
    29161 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
    29221 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
    29281 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
    29341 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
    29401 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
    29461 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
    29521 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
    29581 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
    29641 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
    29701 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
    29761 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
    29821 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
    29881 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
    29941 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
    30001 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
    30061 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
    30121 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
    30181 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
    30241 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
    30301 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
    30361 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
    30421 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
    30481 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
    30541 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct gattttcagc
    30601 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
    30661 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
    30721 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
    30781 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
    30841 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
    30901 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
    30961 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
    31021 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
    31081 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
    31141 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
    31201 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
    31261 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
    31321 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
    31381 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
    31441 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
    31501 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
    31561 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
    31621 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
    31681 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
    31741 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
    31801 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
    31861 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
    31921 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
    31981 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
    32041 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
    32101 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
    32161 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
    32221 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
    32281 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
    32341 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
    32401 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
    32461 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
    32521 tttatttttt taa
//
LOCUS       DAFCNS010000031        31894 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFCNS010000031 DAFCNS010000000
VERSION     DAFCNS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.031"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(25..600)
                     /locus_tag="LNE23_001323"
     CDS             complement(25..600)
                     /locus_tag="LNE23_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6931186.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(605..1579)
                     /locus_tag="LNE23_001324"
     CDS             complement(605..1579)
                     /locus_tag="LNE23_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HBK6931187.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1603..1647)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1862..2830
                     /locus_tag="LNE23_001325"
     CDS             1862..2830
                     /locus_tag="LNE23_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6931188.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2927..3220
                     /locus_tag="LNE23_001326"
     CDS             2927..3220
                     /locus_tag="LNE23_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK6931189.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3338..3574
                     /gene="secG"
                     /locus_tag="LNE23_001327"
     CDS             3338..3574
                     /gene="secG"
                     /locus_tag="LNE23_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBK6931190.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3677..4432
                     /locus_tag="LNE23_001328"
     CDS             3677..4432
                     /locus_tag="LNE23_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6931191.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4435..6792
                     /gene="rnr"
                     /locus_tag="LNE23_001329"
     CDS             4435..6792
                     /gene="rnr"
                     /locus_tag="LNE23_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBK6931192.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6814..7278
                     /gene="smpB"
                     /locus_tag="LNE23_001330"
     CDS             6814..7278
                     /gene="smpB"
                     /locus_tag="LNE23_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBK6931193.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7580..8299
                     /gene="atpB"
                     /locus_tag="LNE23_001331"
     CDS             7580..8299
                     /gene="atpB"
                     /locus_tag="LNE23_001331"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBK6931194.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8352..8567
                     /gene="atpE"
                     /locus_tag="LNE23_001332"
     CDS             8352..8567
                     /gene="atpE"
                     /locus_tag="LNE23_001332"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBK6931195.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8658..9182
                     /gene="atpF"
                     /locus_tag="LNE23_001333"
     CDS             8658..9182
                     /gene="atpF"
                     /locus_tag="LNE23_001333"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBK6931196.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9169..9711
                     /locus_tag="LNE23_001334"
     CDS             9169..9711
                     /locus_tag="LNE23_001334"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBK6931197.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9734..11290
                     /locus_tag="LNE23_001335"
     CDS             9734..11290
                     /locus_tag="LNE23_001335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBK6931198.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11304..12206
                     /locus_tag="LNE23_001336"
     CDS             11304..12206
                     /locus_tag="LNE23_001336"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBK6931199.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12229..13635
                     /gene="atpD"
                     /locus_tag="LNE23_001337"
     CDS             12229..13635
                     /gene="atpD"
                     /locus_tag="LNE23_001337"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBK6931200.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13649..14071
                     /locus_tag="LNE23_001338"
     CDS             13649..14071
                     /locus_tag="LNE23_001338"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBK6931201.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14540..14770
                     /locus_tag="LNE23_001339"
     CDS             14540..14770
                     /locus_tag="LNE23_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBK6931202.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14867..16168
                     /gene="murA"
                     /locus_tag="LNE23_001340"
     CDS             14867..16168
                     /gene="murA"
                     /locus_tag="LNE23_001340"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK6931203.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16181..16357
                     /locus_tag="LNE23_001341"
     CDS             16181..16357
                     /locus_tag="LNE23_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK6931204.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16492..16788
                     /gene="yidD"
                     /locus_tag="LNE23_001342"
     CDS             16492..16788
                     /gene="yidD"
                     /locus_tag="LNE23_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBK6931205.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16852..17319
                     /locus_tag="LNE23_001343"
     CDS             16852..17319
                     /locus_tag="LNE23_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6931206.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17404..18288
                     /locus_tag="LNE23_001344"
     CDS             17404..18288
                     /locus_tag="LNE23_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6931207.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18402..18638
                     /locus_tag="LNE23_001345"
     CDS             18402..18638
                     /locus_tag="LNE23_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBK6931208.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18726..18806
                     /locus_tag="LNE23_001346"
     tRNA            18726..18806
                     /locus_tag="LNE23_001346"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18760..18762,aa:Tyr,seq:gta)
     gene            18813..18884
                     /locus_tag="LNE23_001347"
     tRNA            18813..18884
                     /locus_tag="LNE23_001347"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18845..18847,aa:Gln,seq:ttg)
     gene            19010..19180
                     /locus_tag="LNE23_001348"
     CDS             19010..19180
                     /locus_tag="LNE23_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931209.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19400..20107
                     /locus_tag="LNE23_001349"
     CDS             19400..20107
                     /locus_tag="LNE23_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6931210.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20166..20810)
                     /locus_tag="LNE23_001350"
     CDS             complement(20166..20810)
                     /locus_tag="LNE23_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBK6931211.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20937..21224
                     /locus_tag="LNE23_001351"
     CDS             20937..21224
                     /locus_tag="LNE23_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931212.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21332..21652
                     /locus_tag="LNE23_001352"
     CDS             21332..21652
                     /locus_tag="LNE23_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6931213.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21970..23046
                     /locus_tag="LNE23_001353"
     CDS             21970..23046
                     /locus_tag="LNE23_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK6931214.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23043..23762
                     /locus_tag="LNE23_001354"
     CDS             23043..23762
                     /locus_tag="LNE23_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBK6931215.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            23890..24441
                     /gene="raiA"
                     /locus_tag="LNE23_001355"
     CDS             23890..24441
                     /gene="raiA"
                     /locus_tag="LNE23_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBK6931216.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            24663..27197
                     /gene="secA"
                     /locus_tag="LNE23_001356"
     CDS             24663..27197
                     /gene="secA"
                     /locus_tag="LNE23_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBK6931217.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            27323..28424
                     /gene="prfB"
                     /locus_tag="LNE23_001357"
     CDS             join(27323..27394,27396..28424)
                     /gene="prfB"
                     /locus_tag="LNE23_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBK6931218.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            28529..29215
                     /gene="ftsE"
                     /locus_tag="LNE23_001358"
     CDS             28529..29215
                     /gene="ftsE"
                     /locus_tag="LNE23_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBK6931219.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            29199..30092
                     /locus_tag="LNE23_001359"
     CDS             29199..30092
                     /locus_tag="LNE23_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931220.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            30244..31692
                     /gene="cls"
                     /locus_tag="LNE23_001360"
     CDS             30244..31692
                     /gene="cls"
                     /locus_tag="LNE23_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK6931221.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT        10606 a         5564 c         6908 g         8816 t
ORIGIN      
        1 ttttgctaat ccagctctgt ttattcagcc tatttttctt tttgaatata tcaagcgttg
       61 tggcaacata gtgatgacta atgaaacaac gacgatagac aacaaacgat caaggtagtc
      121 tgtacctgcc tgtaccaaga tcacacttgt tgtctgactt aaacctaacc catgtaatag
      181 ctgaacgatc atactggatc ctgaggaggt gatcccgcca aataactgaa cggtaatgat
      241 cgaagaaacg atcgttccag gtaccgacaa aatcaaagcg agtaataaca gtttcaagct
      301 gtttttatct ttgccgagat atcttggcaa aatcaatcca gcaagcaagc ctgtgagaat
      361 ttggattggc gaataataca gagaaaaaat atctgtagtc actccactta aaataccgct
      421 ggacaatcct gtcagcattc caggtacagg cccaagcaat gcaccagcaa aaatcgttcc
      481 gatagaatcg agatagattg gcaaacgaag ccataaagca atcgttcctc caatataatt
      541 gatcccaata cacaaagcca tcactgtaag tacgcgagtt gccattttgt ttttattcat
      601 tttgttagcc catctttaac ctttccaaat ctttcataat cgtttcttct tgtgcattta
      661 aaagaacggt taaaaacttt cggaaaaaaa gacgagtatc tacttttgtc ataattttac
      721 tgttagcagg tttctgccaa aattcataac gatcaaccaa tgtttgtcca cgacttattc
      781 cttgagtttc cacagcagtg aagcttttga aaccttcaca gatggcctcc tcgataaaat
      841 aagcaacagc taacggatca ttgatgacac atcctaaaat tctttcttgt ttccagtgga
      901 agtcaaaata aaaacgagtg atcgctttta gatactctcc ctcttcagga ttcatttgac
      961 agcagtattc gaggatagtc ggtgtcaaga cgatttctct tgtcacatct aacccaacca
     1021 tttctatcgt ttgtttgaga ttctcgaata cataagaagc tgcttcagga tcacaccagt
     1081 aattgtattc agcaactggc gaacagttac catggctttt gtaagttcca cccatcgaga
     1141 caaaacgttc catatgtttc ccaatatttg ggttgacttt caaagcagaa gcgatattgg
     1201 tcaaaggacc caaagcaatg actgatgtat ttgtcttttc tttgaaataa tcagccaaaa
     1261 aatcaacggc atggagaggt tctgcctgtt ttctcaagat catcgggaaa tttgtttccc
     1321 ctaaaccatc cataccatga gtgtcttgtg cactgatgaa aggttgcttc aaaggttttt
     1381 ccattccttg ataaacagga atgtctaaac gatcacagcg ttccaggcat ttcagcacat
     1441 tctgcgtacc tatatctgca gggacattcc cacaaactac ggtaatagca actacttcga
     1501 tatcagggga tttgacagct aatgataaag ctaaagtgtc gtcgatccct ggatcgcaat
     1561 caataattac ttttcgcatt atttccaatc tcctttcttt agagatactt agttttttat
     1621 tctgggaagt ttccgaacca gtccaagacg agcgtcttga ttataatgca cgaaattagt
     1681 atactggctt ttattgcgaa tgtaaatgct ctcttacaag taaatctatt gaggagaact
     1741 ttaagtaaat gatacaatga aaaagaaatg gaatttacgt gtgccgaaaa taaagaatcc
     1801 tatggatcgg tacattggat aatagataga ttatagtttg gcagaaagcg gcgggtgtac
     1861 aatggttatt ttttatttca ttcttattct gataattata gggatcagtt accattttct
     1921 ttcttatttt tatgcaaaga aattagtaag agcgggagta cagagaggat atgactggta
     1981 tgaagacact ggtcatcgtt tgatggaccc tgctgcagtc acgtcagtag aaaaaaaacg
     2041 acaaaaatta gaagaagagt gtgaaagctt ttgggagcag ggaacaccca aaacaatcaa
     2101 aagttatgac gggttgaaac tagctggtca gatgttcatc catccaaata agcagaataa
     2161 atgggtcatt tgcgtacatg attaccgaag cacagggaaa agagacatgt ctcatatagg
     2221 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
     2281 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
     2341 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
     2401 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
     2461 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
     2521 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
     2581 ttactcctta aagcaagcat cagctacgag acagcttggc agcaatcatc tgcctttatt
     2641 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
     2701 tgctacggca ggggataaag cactgctgct tgttccggaa gcagaacact tggaagcatc
     2761 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
     2821 gatgaattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
     2881 tcaggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
     2941 atatggaaat caaagaagaa aaaatcgtct tgctttattc aatgatgaac aacaagagat
     3001 cggagaaatg acatggtcag atgcgggccc agacatcatg atcgttgacc atacatttgt
     3061 agatccagca tatcgtggtc aaaagttagc tgaaaagcta gtgtatactg gagtagaatt
     3121 agcaagacga gacgggaaaa agatcattcc tttatgccct tatgcaaaaa aagagtttga
     3181 aaagaaacca gaatatcaag acgtcttacg tcagtcataa gaataacagc tgaaagtttt
     3241 gaactttcag ctgttattcc atttatatag agttgtcacg gacttttatc tatgataaag
     3301 ttaaagagac gatatctttt gggaaggagg ctgcgatatg tataatttaa ttttgggaat
     3361 cgtgatcgtt ttatcgattg ttatggtcat tgcgattatg atgcagccaa gcaagcaaaa
     3421 tagtgcagcg agtgcattta ccggcggtgc ggatcaacta ttcggcaaac aaaaagctcg
     3481 cggctttgaa gctgtcatgc agcgatcaac tgcagttatg ggagctatct ggatgatctt
     3541 attatttgtc ctagcatatt tatcttcgaa gtagttgaat aagcctctcg caaaacggcg
     3601 ggcggctttt tttattgcaa agagaacgct taagaagaag ttatgaaaga aactatggta
     3661 aaatgaggcg aaagaggtga gacgagtgag acaattacca aaaccccttt atgcaaaaca
     3721 tggaaagcga gcggttttgc tgcttcacgc ttactcagga agtccaaacg atgttcgaat
     3781 gctcgcacga tttttggaaa aagcagatta tacagtttat gcgccgcttt ttaaaggaca
     3841 cggaacaatg gagccatatg atatcttgca agaaaaagca gaaagctggt gggcagacac
     3901 taaaaaagcc atccattttt tacagtctga gcagttttct gatattgccg tattaggatt
     3961 atcaatgggc ggcatctttg ctgtacgagc actagaagaa gaatcagtga ttggcggcgg
     4021 ttttttctgt tcgcctttgt ctcctgtaaa aacaaacgtg cctgaaaact ttgaaaaata
     4081 cgtacgtcaa gtgctgaaga cagcaggtaa atcagagaaa gaaatcaatg aaaaagcagt
     4141 tgcttaccgc tcgttagctg aacaacaatt aatggatata caagatcagg cagcaatcgt
     4201 agaaagtagg ttgtcagata tccaacagcc gatattttta gctcaagctg ggaaagatga
     4261 aatgatcgac cctaatggcg tatttgaaac tgcacgcaag ttgagccgtc agcgagtgac
     4321 acttcaatgg tatcctgaaa gtggtcatgt gattacagtt ggtacagcaa gacgcgaatt
     4381 agaaaaagac gtcttggaat ttttagaaaa attaccttgg aatgaggaat aaatatgaca
     4441 aaagaaacga taaaagagaa aattctcttt ttcatggaaa gcagtcgcaa acgcagtttt
     4501 tctatggaag aaatcgctga aggtttaggg ttccaaaaaa gcgatgattt taaattacta
     4561 gtacaaacga ttgctcaaat ggaaagagaa cagagtatag ttttcaataa aaaaggaaaa
     4621 gtgaaacttc ctttaaaacc aatcctgatc gaaggaacat ttcgagcaaa tgaacgagga
     4681 tttggatttg taacgattga tcccgaagaa gatgacatct atatcccaaa agaagcgact
     4741 ggttttgcaa tggatggaga caccgtagca atcgatatca tgaagcgagc aaatacagat
     4801 atggatcgcg gagcagaggg gaaagtagtc gagattcgag accgggcgac tactcagtta
     4861 gcaggggaat tcgttgctta ttcagaagaa gaaatacaag aaacagatct ttacggcgtt
     4921 gttatcccga aagatagaaa gctcaaccaa tttaaagtat atgcagcagc agagggagtt
     4981 cgtccagttg acggaagtat tgtgctgatc gaactgaccc attaccctga aaaagggtat
     5041 gcgactagtt tagaagggat aatcaagcaa gtgattggtc ataagaatga ccctggcatg
     5101 gatatcctct caattgtcgt ggctaatggt atccctacga aatttcctga tgatgtattg
     5161 tctgaagcag atgaagttcc agatcaaatc agcgaaaagg atataactgg acgtagagat
     5221 ttaagaaatc agctcattgt tacgattgat ggagaagatg caaaagatct agatgatgca
     5281 gttacggtta aaaaattaga taacggtaat tattttcttg gagtacacat tgcagatgtt
     5341 tcttactatg tgacacaagg cagtcaattg gacagagaag catatgaaag agggacaagt
     5401 gtatatttga ctgatcgagt aattccgatg atcccgcaac gtttatcgaa tggtatctgt
     5461 tcactgaatc cccatgttcc aaggctgacc atgagttgcg aaatggagat tgatccaaat
     5521 ggacaagtag tctctcatga tattttccca agtgtgatcc aaacaacaga acggatgacc
     5581 tacacagctg tcaatcaaat cttagaagat caagacgaag aaacgatgca acgctatgct
     5641 cagctcgtac ctatgttcca agaaatgggt gaattgcacc gaatccttga aacgatgcga
     5701 atcaaacgtg gcgcgatttc ttttgaggat cgcgaagcga aaatccttgt tgatgctgaa
     5761 ggacacccaa atgacatcca gctaagaagc agaggaattg gtgaacgctt gatcgaatct
     5821 ttcatgcttg cggccaatga aacggtagct gaacattata ataaattaaa tctgccgttt
     5881 atttaccgaa tccatgaaca gccaaaagaa gaaaaaatgc agcgcttctt tgattttgct
     5941 tcagctttgg gcattctggt ccgaggaacg aaaggaacga tcacaccaaa agatttacag
     6001 catgtgatcg aaggagtaga agacaaacca gaagctgcag ttatcaatac tatgttgcta
     6061 agaagcatgc aacaagcacg ctattcagaa gacaattttg ggcattatgg cttagcggca
     6121 gattactaca cacactttac atctcctatt cgacgctatc ccgatctaat cgtccatcgt
     6181 ctgatccgtt cttacagtga agataaaagt gaaagcaatc aaagttcgtg ggctgcggaa
     6241 cttcctgaaa ttgcagatca tagttctaag atggaacgtc gtgcagtaga aacagaacgt
     6301 gaagtagatt ccatgaagaa agcagaattc atgatggaca aagtgggaga agaattcgat
     6361 gggatcatca gctctgttgt gaaattcggc ctgtttatcg aactgccgaa tacagtagag
     6421 ggattgatcc atatcaatga attgaaacag gactacttcc attttgttga aaatcattta
     6481 gctcttgtag gtgaaagaac tggtctgact tttaaaatcg gacagaaagt gcgagtaaaa
     6541 gtagtcaaag ctgatccaga tgaacgagca gtagattttg aacttgtttc cgcagaagag
     6601 gttacagcgt tggaacggcc aaaaacacga aatggaaaat caggaaaacg cgaaggaaaa
     6661 caagcaggaa aacgcccaga aagaaatggc aagaagaaca aacgatatag cgatagtaag
     6721 caaacgttcg gtaagaaaaa agggaagaag ccattttaca aaggtgttaa aaagaagaaa
     6781 aagaaaaaat aaaatgagtt ggaggaaaca gctatgccaa aaggcgaggg aaaattaatt
     6841 gcacaaaaca agaaagctcg ccatgattat tcgatcatcg acacgatgga agcagggatg
     6901 gtcttgcaag gaaccgagat caagtcgata cgaaacagcc ggatcaatct aaaagatgga
     6961 tttattcgcg tccgcaacgg agaagctttc ttgcataatg ttcatatcag tccttatgaa
     7021 caaggaaata tttttaatca tgatccgttg cgcacgagaa agttattatt acacaaaaaa
     7081 caaatcatcc ggcttgaaaa tgaattgaaa aatactggaa tcactgttgt tcctttaaaa
     7141 gtctatattc gtaacggcta tgccaaggta ttgattggtc tggcgaaagg gaaaaaatct
     7201 tatgataaac gggaagattt gaaacgaaaa gatatcgatc gacaaattga tcgaacatta
     7261 aaaaatttct ctagataatt tcattatcct ttttcgcata ttcggtgctt ttgcttttac
     7321 ttaatgataa aatttgttag catttaatct gataaaacat gattatcata ggttttttct
     7381 tagatcagga gcaccgtgta taaagaaaaa ttcgcttcgt ttttgactga aataaaaaag
     7441 tgtctaattt tcatgaaaat gttttgaatt ttctgtttca tggtggtatg ataccgtgtg
     7501 gttagaaacc gattggaaaa aaccagatac gcacatgaat tgattttatg tgaatttcgg
     7561 ggaaaagagg tgaaatggat tggatgaacg ctcgctaacg ttccacattg gaccaatttg
     7621 gtttgatggt acagtttgta taatggtgtt actgacctgt ttgattgttt tcttcttagc
     7681 ttactttttt acaagaaatc ttaagttgaa accaacaggc aaacaaaatg cgatagaatg
     7741 ggtcattgat tttagtcgag gaatcgtaac agataatctg cctagaaaag aattgagcaa
     7801 tttccatttg ttggcattta cattgttttt atttgttttt gttgcaaaca acatcggttt
     7861 agtaacaaaa atcgttttac ctaacgaaac aacactttgg aaaagcccga cagcagaccc
     7921 gtttgttact ttaactttgg catttataat gatcttactt actcacttat tcggggtgaa
     7981 aaagttaggc ttcaaaggat atttcgtaaa ttcgttcttg aaaccctata gttttatgtt
     8041 tccgatgaaa ctgattgaag aatttacaaa cttattaaca cttgctttac gtctttatgg
     8101 gaatatttat gcgggggaag ttttgctaac cttgattgca aaaatgatga caagcttagg
     8161 ctggttctca ttaccattag caattccatt agagatggtc tggatcgcgt tctccttgtt
     8221 tattgggagc atccaagcat ttgtctttgt cactttgtca atggtttata tgagccataa
     8281 gattgaagta gaagaataat actcggttaa aaaacaaact actatttatc tattttagga
     8341 ggaaaacaat tatgaattat atcgcagcag caatcgcaat catgggagca gcaatcggag
     8401 ccggttatgg taacggtcaa gttatctcta aaacaatcga atcaatggct cgccaacctg
     8461 aaatgtctgg tcaattccgt acaacaatgt ttatcggggt agctctagtt gaagctgttc
     8521 ctatcttagg ggtagttatc gccttgattc tagtttttgc cgtttaattg aatcagtatt
     8581 aaggcagaaa caacatcgtt tgttactgct tttttacatg gaaactagaa atactccaaa
     8641 gaaaggaagg ctcgcctatg ctgaatcaat tggcaattgc agaagttggc aatgcgatgt
     8701 taggtaatat catcgttgtc agcggctcat ttttgatact actggctctc ttgaagcact
     8761 ttgcttgggg accaatcagc gatattttga aaaaacgtga agacaagatc gccaatgatt
     8821 tagattctgc agaacaatct cgcatcaact cagcgaaaat ggaacaagaa cgcgaacaac
     8881 aattgttagc ctctcgttct gatgcagctg atatcatcaa aaatgcgaaa gaaagtggag
     8941 aattaagccg ccaaaatatt ttgaaggatg ctcaagaaga agcagctcgt ctaaaaagca
     9001 aagcccaagc tgatatcact gtagaacgtg attcagcgct gaactctgta aaagacgacg
     9061 ttgcagaact ctctcttcaa atcgcggaaa aaatcttgaa caaagaatta tctccagaaa
     9121 tgcatgaatc attaatcaat caatacattg aaggtctagg ttcttcaaat gaaactagat
     9181 aaatatacag taggaagacg ttacgggaaa gctttatttg aactggcgat cgattcaaat
     9241 caagcagaag aaatctatca agatctcgta agtttacgtc aaatctatga acaagtacct
     9301 ggattaggga atatgttaag tgatgttcgt ctagaacctc atgaaaaacg agaaatcatg
     9361 gacaagcttg tcagcggtta tgatgggatc gttaaaaact ttttagaagt tgtttatgag
     9421 tataaccgaa tggatgacct tttgttcatg atcgaagaat atgaacgtcg atatgatgaa
     9481 catcaagggt tactattggg aagtgtcaca acagcagtcc cactttcaga cgaacaacga
     9541 atacaattag aaaagaatgt tgcaaaaaca atggactatc aatcagttga attaaaacaa
     9601 atcgttgatt cttctattat tggtggcgcg attgttgaag ccaaccatcg agtaatcgac
     9661 ggaagcattc gcacgcaatt agaaaaaatg cgtaatcaat taaacagata gtacaaagag
     9721 gtaggtgaat cgaatggcca tcaaagcaga agaaattagt gccttgatta aagaacaaat
     9781 tgaaaattat caaaacgtgc tttcagtcga agaaatcggt accgtaactt atgtaggtga
     9841 cgggatcgct cgtgcacatg gtttagaaaa cgcgatgagt ggtgagttgc ttgaattttc
     9901 aaacggctca tacggaatgg cgcaaaactt agagtcaaat gatgtaggta tcattatcct
     9961 tggcgatttc gagtccattc gtgaaggaga taaagtaaaa cgaacaggta agatcatgga
    10021 agttccagtt ggagaggcct tgatcggtcg ggtagtcaat ccgctaggtc aaccaatcga
    10081 cggactaggt gaaatcgtta ctgacaaagc tcgtccagta gaagcgatgg caccaggtgt
    10141 tatgcaacgt aaatctgtta acgaaccaat gcaaacaggg ctaaaagcga tcgacgccct
    10201 tgtgccaatc ggacgcggac aacgtgaatt agtcatcggt gaccgtaaaa cagggaaaac
    10261 ttctattgcg atcgatacga tcatcaacca aaaaggtcaa gatatgatct gtatctatgt
    10321 agcaatcgga caaaaagatt ctacagttcg tacacaagtt gaaacattga aaaaatatgg
    10381 cgcaatggat tacacaatcg ttgtgaatgc cggtgcgtct caaccagcac cattgcttta
    10441 tatcgcacca tatgctggta ctgcaatggg tgaagaattc atgtacaacg gtaaacatgt
    10501 attgatcatt tttgatgatc tttcaaaaca agctgttgct tatcgtgaac tttccttgct
    10561 acttcgccgt ccaccaggtc gtgaagctta tcctggggat gttttctact tgcactcacg
    10621 tttattagaa cgtgcagcaa aattaagtga tgatttaggt ggcggttcaa tgactgcctt
    10681 accatttgtt gaaacacaag ctggagatat ctctgcttat atcccaacaa acgttatttc
    10741 gatcactgat ggacaaatct tcttagaaag cgacttgttc tacgcaggta ctcgtccagc
    10801 ggttgatgcc ggactatctg tatctcgtgt aggaggttct gcgcaaatca aagcaatgaa
    10861 aaaagttgct gggactttac gcctagactt agcaagttac cgtgaattag aagcatttac
    10921 acaattcggt tctgacttgg atgcagcaac acaagcaaaa ctaaatcgtg gacgccgtac
    10981 agtagaaatc ttgaaacaaa aattgcatgc accgctagct gttgaaaaac aagtagtgat
    11041 tttgtatgct ttgacacatg gattcttaga tagtatccct gtagacagta ttttagactt
    11101 cgaacatgaa ttattcgaat atctggatac caatcattca gatatctttg aaacaatccg
    11161 tacaacaaaa gatcttccag aagaagaagc gttgaacagt gcgattcagg aatataaaga
    11221 catgttctta gctacaaaag gaagtaattc ctctacagaa gataaattga aatccattca
    11281 aaatgcatag tgaggtgaga tgaatgggtg cttcattaaa tgaaatcaag acacgtatcg
    11341 cttcaacgaa aaaaacgagc caaatcacgc gggcaatgca gatggtgtct gcttctaagt
    11401 tgacaaaatc tgaagcttct tcacagaagt ttcaggttta tgcaacaaaa gtaagagaaa
    11461 tcgttacgca tttgactgct actcagttaa atgatattgc ctcagaaaat cctagagggg
    11521 atatcaatta caacagcatg ctgctcagcc gtcctgtgaa gaaaactggt tatatcgtca
    11581 tcactgcaga tggcggttta gtcggtggat ataacagctc gatattgaaa caaacgatgt
    11641 ctattttaga agaagaccac caatcacccg atgattacgt gatgatcgca attggcggaa
    11701 ctggtgcaga cttctttaaa gcacgcggta tcaatctagc atatgagctg cgcaatctat
    11761 ctgatcagcc aagctttgat gaagtccgca aaattgtaaa tatggcaaca actatgttcc
    11821 aaaacgaagt attcgatgaa ttatacgtat gttataacca tcatatcaat tcgctaacta
    11881 gtcagtttcg tgtagaaaaa atgctgccaa tctcagactt agatccagaa gaagcaacga
    11941 ctttcgaaca agaatatatt tttgaacctt caaaagaaga aatcttgtct cagttattgc
    12001 ctcagtatgc tgaaagtttg atctacggtg cgatcgttga cgccaaaaca gcagaacatg
    12061 ctgctggtat gacagcaatg aaaacagcaa ctgataatgc tgctaatatc attgatgagc
    12121 tgacggtttc ttataaccgt gcaagacaag gggctattac ccaagaaatt accgaaattg
    12181 ttgccggagc ttcggcacta gaatagtatt gagattggag gaaaaaagat gagttcaggc
    12241 aagattgttc aagtaatcgg tcccgttgtc gacgtggaat tttctttaga ccaatcctta
    12301 ccagatatca acaacgcatt agttgtttat aaaaatgacg aaaataaatc aaaagttgtt
    12361 cttgaagcag cgttagaatt aggggacgga gtgatccgga ccattgcgat ggaatcaaca
    12421 gatggtttac aaagaggaat ggaagtcatt gatacaggca aagcaatctc tgttcctgta
    12481 ggtaaagaaa cattaggtcg cgtattcaac gtactaggag atacgatcga tttagaaaca
    12541 cctttcccag aagatgcgga aagaagcgaa attcataaaa aagcaccagc ctttgacgaa
    12601 ttaagtacaa gtacagaaat tttggaaaca gggatcaaag ttatcgattt gcttgcccca
    12661 tatttaaaag gtgggaaagt cggactattc ggtggtgccg gtgttggtaa aaccgtactg
    12721 atccaagaac tgatccataa tatcgcccaa gaacatggtg gtatttctgt atttaccggt
    12781 gtaggtgaac gtactcgtga aggtaatgac ttgtattatg aaatgaaaga ttcaggagtt
    12841 atcgaaaaaa cagccatggt gttcggacaa atgaacgaac caccaggtgc acgtatgcgt
    12901 gttgctttga ctggtttgac gattgcggaa tatttccgtg atgtagaagg tcaagatgta
    12961 ctgttgttta tcgacaacat tttccgtttc actcaagctg gatctgaagt atcagccttg
    13021 ttaggacgga tgccttctgc ggttggttat caaccaacat tggcaacaga aatgggtcaa
    13081 ttgcaagaac gtatcacatc tacgaaaaaa ggttcgatca catcgatcca agcaatctat
    13141 gtgccagccg atgactacac tgacccggcg ccggcaacag cattcgccca tttggatgcg
    13201 acaaccaact tggaacgtaa attaacagaa caagggattt atccagcggt agatccattg
    13261 gcttcttctt caagtgcttt agcacctgaa atcgtgggag aagaacatta taaagtagca
    13321 acagaagtcc aacacgtatt gcaacgatat cgtgaattac aagatatcat tgctatctta
    13381 ggtatggatg aattatccga tcaagaaaaa gtgttagttt ctcgtgcccg cagagtccaa
    13441 ttcttcttgt cacaaaactt taacgtggct gaacaattta ctggtcttcc aggttcatat
    13501 gtccctgtag aagaaacagt gaaaggattc cgcgaaatct tggaaggcaa atacgatgat
    13561 cttcctgaag aagctttccg aagtgttggc cgaatcgaag acgtcgtaga aaaagcgaaa
    13621 acactaggct actagaaagg ggtgccccat ggatcaatat ttaacagtaa atgtggtaac
    13681 tcctgccggt ttggtctatg atcaccatgc agctatcgtc gttgcacgta cgacagctgg
    13741 tgagcttggg attttgccta aacatgcacc aatcatcgtt ccattgacga ttgatgaagt
    13801 tcgtgtaaaa cgaacagatt cagataccca cgtcgattgg atcgctgtta atggcggaat
    13861 catggaagtt cgtgataatg ttgtttcaat cgttgctgat agtgcggaac gtgaacgtga
    13921 tatcgatgta tctcgtgcgg aacgtgcaaa acaacgcgca gaacgccaaa tcgaagaagc
    13981 aaaagcaaaa caagatacga acgaattgaa acgggcaact gttgcgttgc accgagcaat
    14041 caatcgtatc aaagtatcta agcactcata aaatgagatg cggatgttta tccgtgttta
    14101 agaacagtaa aaagaggtta tgacagaagt ggtcagcttc aagacataag aaggaattta
    14161 cgaaaattgc ttctcaaatt tttgtgaaat ctcagcttat ttttcgaaga agctacttct
    14221 ggaacaccgt atatcagagg ttgtgaaatc agcgttttgt cctgagtatt agagaaacaa
    14281 atggaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagatgatt
    14341 gaacaccgtt tatttggaat aagggactgc gacaagactt ttgtctcagt cccttttttt
    14401 gcatattttt agcatttatt tcctaaaaaa tgtaagggga ttcctatatt tttaagtata
    14461 cttgtttttc cttttataca ttttatggta ttatcatttt attagttatt ataaaacaat
    14521 cgttagaaag gacgtcatta tgcaagtata cggaattgat gcggtgcttc gaatcgtgag
    14581 tcatttggcg tttatctact tagccttctg gtctttacgt tcgttacgca ttgaaacttt
    14641 tttcagacca atgcataata cgcaaattag aatggcaatc gtactttttt caattttttt
    14701 agggtacact gcaagtaatt ttttcttaga ggttatcgct ttatgccgaa atcttttcgt
    14761 tagcctgtaa attttaaaaa aaggtactat tttaaaagca aggatgttat aatagaaaag
    14821 attttgagta agtgtatgct cattggaatt ttttggaggg aacaacatgg aagagatcat
    14881 cgtaagaggt ggcaatcaat taaatggaac agtaagaatt gaaggagcta aaaatgctgt
    14941 tttgcctatt ttagctgcta gtttgttagc agaagaaggt attacgacat tagacaacgt
    15001 tccgattctt tctgatgtct tcactatgaa tcaagtgatt cgtcacttaa acgtagatgt
    15061 agattttgat gaacaaaaga atcaagtaac aatcgacgct tcaagacaat tagaaatcga
    15121 agcgccttat gagtatgtta gtcaaatgcg tgcttcaatc gttgtgatgg gtccgctact
    15181 tgccagaaat ggacatgcaa aagtagctat gcctggtgga tgtgcgattg gtaaacgacc
    15241 aatcgatttg catttaaaag gatttcaagc tttaggagca aaaattattc aaaaaaacgg
    15301 ctatatcgaa gcaattgcgg atgaattgat cggtaacacg atttatttag atttccctag
    15361 tgtaggtgct actcaaaata tcatgatggc tgccgtaaaa gcaaaaggaa caaccatcat
    15421 tgaaaacgta gctcgcgaac cagaaatcgt tgatttagca aacattttga ataaaatggg
    15481 cgctcaagtc tatggagctg gtactgaaac aatgcgtatt gaaggcgtgg atcatttaca
    15541 tgcagtcaac cactcaatcg tacaagatcg tatcgaagca ggtaccttta tggttgctgc
    15601 tgcaatgaca caaggcaatg tgctgattgc agatgctatc tctgaacata accgcccatt
    15661 gatttctaaa ttgatcgaaa tgggtgcaga aatcattgaa gaagaaggcg gagtccgtgt
    15721 catcggacca aaacatatct tacctacaga tgtaaaaacg atgcctcatc ctggtttccc
    15781 tacagatatg caagctcaaa tgacagctat ccaattagtt gcagaaggta caagtgtggt
    15841 gactgaaaca gtgtttgaaa atcgtttcca acatttagaa gagatgcgcc gtatgaacgc
    15901 tcatgtcaaa atcgacggaa atgtagcaat catggatgga aaccatgaat tgcaaggtgc
    15961 agaagtatat gcaacagact tgcgggcagc agcagcatta gttttagctg gcttgaaagc
    16021 aaatggaatc acacgtgtgc gtaacttgaa ttatctagac cgtggatatt ataatttcca
    16081 tatcaaacta cagcaattag gtgcagacgt cgaacgtgta gacatggatc aaacttctgc
    16141 tgaaaaaaca gcacaaacga ttgcttaagg aagtggctga atgagtaacc aacgatatat
    16201 cttattaact ttgataaaga ttttagtggt gatcttattg cttatccttc tttttgtggc
    16261 cggaacgatg atcggatatg gtgtcatcgg tggcggcaat ccatttaaag tattccagcc
    16321 aagtttgtgg attcatattc gagacttttt tcattgataa ataagaatca cagtggttct
    16381 agtcgatatg aagtgagcca tgaagcagaa catgataaaa gaaaacagaa gggaacgttg
    16441 acgttagtcg cgatcccttt ttttagtaaa agaaaagaag gaatctaatt gatgaagaag
    16501 atattgattt atttagttag aggatatcag cgctttattt cgcctgcatt tccacctagc
    16561 tgccgttatt acccaacctg ttccaattat atgataaaag caattcaaac ccatggtgcg
    16621 ctaaaaggga cggtaatggg gactgcacgg atcttacgct gtcatccttt tgtcaaaggc
    16681 ggtatcgatt atgtaccatt gacattccga ttaacgcgaa ataagaaaga taaaaaagaa
    16741 ccagattata aaaaggttgt gaaaaatacg ggtagcactg agcgataaag aaaaagagga
    16801 aatttattgt caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa
    16861 gaactaaaca agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa
    16921 aaagttcaag tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca
    16981 ataggtgtac tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta
    17041 accccaacgt atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag
    17101 ctttcacagc tagcttcaaa ccaaactcgg atcattatac gtactggcac tactagtagc
    17161 gctgcgttac acctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat
    17221 tttatccatc attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg
    17281 attttagcaa ttcacttacc taaaaatagc tttaactaat aaactttcaa ttcgagaaaa
    17341 tgctttcaaa tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt
    17401 tttatgggaa tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat
    17461 ggcaaagaga ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa
    17521 acgcaattat ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga
    17581 atcagctctc ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt
    17641 ccttatattc atggatttaa tattttccga gatttggaaa ctctgtttca actcccagta
    17701 acgattgaaa atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgt
    17761 gattatcaag atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg
    17821 aaaggacaaa taaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta
    17881 gatggcgata agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt
    17941 gaacgcaaag gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa
    18001 actggagatg caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga
    18061 ttattcagta ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct
    18121 gcaaaagaag ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc
    18181 gatctgcatg atttcgaacc gcagatttta ttatgtgagt acagaaacga tgctaatctt
    18241 ataggtgcag cagcaaacta tctcgctgta cgtcaaggag agaattaaga gctttagaga
    18301 aggtgcagct tgcataaaag aagagaatga aatgaatagg agatttttac ctaaggaatg
    18361 ttttatttgc tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata
    18421 aagatattga agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag
    18481 tcactcagtt atttataggg aaaaaaatga ttggtgagat cttagcctat gggccaaaag
    18541 aatatgaaat tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga
    18601 tagaaacagt gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca
    18661 ataaagggtt gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac
    18721 ctttcggagg agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt
    18781 cagtggttcg aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg
    18841 gactttgact ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa
    18901 gctctcgtta gttcgagagc tctttttttt tgctttgcta ccttgttcct tgaagattct
    18961 gctgaattaa gatagagtta agttaactac aagaattgga gggatccaca tgaaatggaa
    19021 agaaaaagta gacgaagcag ctgaaaagtt atatgatttg gtcaaatcag aaaaatacaa
    19081 tattgaagtg aatattccga aaaaaggcgg aaaagcggtc caggtaaaat caaaacgccc
    19141 aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa taataacaaa agatccataa
    19201 gtcggcattt tcatcgattt tagggtcttt tgttattttc taggttgaac atatctaaaa
    19261 aattgtttct gacaatagga gtagggagaa cgatgtgtgt ctaaatcctc tgttgatgga
    19321 tagaaacctt tatctttttc agttgtgaat caggtatata ccagttataa tagaagaaac
    19381 gaaaggacgg gtgtacgtta tgtcgaatca attacctgta tatatacaga ttcatgacca
    19441 attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga gaccgtcttc cttcagaaag
    19501 agaattagca gtgaaattca atgtcagccg tatgacgttg agacaagcaa ttcaaaactt
    19561 ggcagatgaa ggaatcttag aaagaaagat cggttcgggt acgtatgttg cgcgagaaaa
    19621 agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa attatggaat cacaaaatcg
    19681 agtaccgtct agtcggacaa tctcttattt cttgacttcg ccaagctcaa gtgaaatgga
    19741 aaaactaaac ttgagtaaag atgacacgat cttgcgaatg gaacgtattc gtttcgcaga
    19801 tgatgtccct atttgttttg aagttgccag tgttccacaa aagctgatcg atggctacag
    19861 caaagcagag attactgaat cgttgtatcg cacgctagaa gaaaaaggcg ggaaaaagat
    19921 cggtccagcg aatcaaacga ttactgctgt gttggcttct gagaaaattg ctgactattt
    19981 agaggtaaaa cgaagagatg cagttcttcg tttgcgccag atttcttatt ttgaagacgg
    20041 tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat cgttttgagt tttatttgga
    20101 aaaataaaaa acaggtgatc cctgattcct tattagaaat aaaagagaat cagaatcacc
    20161 tgtttttata tatgatcagt tatcagttgc tcgtgataag aaacagaacc ctcttcaaat
    20221 tggacttgtc cgttcagcag ctcaatgatg gattctttaa agtggttagc acttttttca
    20281 tctacagcac agatgacgct aacttgatca gtatattgga tttcttttat aaaaaaatgt
    20341 tggagttcaa gataattctg taattttcct agttgcggat acgtgatcat cacagagatc
    20401 tcctgttgca aggtaccttc tacgattcca atgtcagaca atgcttgaga gaccgcatga
    20461 gtgtatgctc gtattaatcc gcctgtgccg agtttcgttc ctccaaaata tcgagtgact
    20521 actgcagcta cattgatcag ttgatttttt ttgaggactt ccaacatcgg tacgcccgcc
    20581 gttccacttg gttctccatc atcgctgctt cgctggattt catttttttc tccaatgatg
    20641 aaggcgctgc agttatggtt cgctttccaa tgttcttttt ttatttcttg aatgaaattt
    20701 tttgcttggt cctctgattc tattcgtcta agagaacaga taaagcgtga ttttttgatc
    20761 tcaatctcac tttgtccatt ttcttttatt gtacgatagc gctcgatcat cttgtcacct
    20821 ctttttttca ttttctttat tcagtataaa taaagttcta ggttgtttca acactgtaac
    20881 ggttgcgcaa ttgctgtagt atgcgataat aaagacaaag gcaggtggat ctggggatgc
    20941 aggaacgaat gaaaaagtac gatgcaatta cgcattattt gaagaacaat ggcggctctc
    21001 aagtaacgtt gacctttacc caatttgacg aactgctgtt tcccagtaac ggacttccca
    21061 aaacagcaag ggaaagtaca gattggtggg caaatgatta taaacaccct gaaaaaggtg
    21121 cctatggctg gataaatgca ggctatgaag tagttgtcat caatttggac aaagaatatg
    21181 tcgtgttcaa taaattagtg aaatctagtt ggctatttga ttaaataaac cgcgtttaga
    21241 aaacgttgac aaatttcttt ctttttatat aataaagtaa cagacgcgaa gaaaacgttt
    21301 gcgtagtttg ttttaaaaag gagagaaaaa tatggctaaa aaaacaatca tgcttgtatg
    21361 ttcggctggt atgagcacaa gtttactagt aacaaaaatg caaaaagctg ctgaagcaaa
    21421 aggaatcgac tctgatattt tcgctgtgtc cgcttctgac gcagataaca atcttgcaaa
    21481 taaagacgtg gatgtgttgt tattaggccc acaagttcgc tttatgaaag ctgattttga
    21541 gaaacgtctg gaaccaaaag gcattccttt agatgtcatc aatatggctg attatggtat
    21601 gatgaatggc gaaaaagtgt tagatcaagc aatcgcgctc ataggtgaat aaatagaaca
    21661 gtataactat cgaacaagcg atccaaaaaa tagatcgctt gttttttcgt atattttttt
    21721 agggcgaata atatgaatca tttttggggt agaaggcttc tgacgagaga tattaaagca
    21781 atagactgtg aggaggtaaa tttaccaaat tatgagaaga tcgcagcagt ggaacgtttg
    21841 ggcaactgga ttctttgtca tcgctgtgga atgaggacgt ccgtcattgt tggacagctt
    21901 gcagattacg gttatttttg tacacattgt ctatatttag ggtgatgcga cactcaacaa
    21961 gatttatatt tgtttgatca gcctgaagca aaacctcgag aagtcgtctt tttttggaca
    22021 ggacagctta ctgaaaaaca aacggaaatc gcaaagagaa tactttgcca ttcagaaaaa
    22081 agacatcatc ttatttgggc tgtaacaggt gcagggaaaa cagagatgct ttatcctatt
    22141 ttagtggaaa ctttaaaagc tggaggaaga gtagcgattt gtacaccgcg agtcgatgtt
    22201 tgtaatgaat tgtttttacg ctatcgttat gtttttccag aagaaaaaat cacgttgctc
    22261 catgggaata cagcaacggc ttataccttt acttcttttg ttatctgtac gattcatcag
    22321 ctataccgtt tttatcggtc ctttgattta atagtgatcg atgagatcga tgcgtttcct
    22381 tataacgggg atgctgggct agctcacgct gtgcaaacag caatcagatc aaacggccga
    22441 ttcatctact taagtgccac gcctgatgag aagttactaa aggaaatcaa gcagacattt
    22501 gagctccaca aacttgcctt gcgctttcat cggcgcttgc ttcctgaacc cgtgcttgtt
    22561 ttcatgaata attggcgaca aaaatgtgga aacaaaaaga aaatcaactc tttggtgcgg
    22621 atcattcagc aactaatcaa agaaaaccgt atccttttat tttgtccaag tattacgatg
    22681 atgcatcgtt taaaaatcac actgcaagaa gcttttccag aatatgagat aacagatgtt
    22741 tcttcacaag acgatcaacg ggcaaagaaa gttcagaaaa tgcgagaagc aatatacgag
    22801 atactgttaa ccacaatgat cctagaaaga ggagtcacat ttgaaagaat ctctgtcatc
    22861 gttttaggag cagatcatcc agtttttact aaatcctctt tggtgcaaat cgcaggaaga
    22921 gctgatcgta aaggtcctta tacgaacagt cgagtctatt ttttctatga agaaaaaact
    22981 tcagctattc gaaaagcctg tcaggaaatc aaagaaatga acgaagaagg gaagcagatg
    23041 ctgtgagatg tagttgctgc ggtaaagaca tcattcaaaa tttaacattc agcgagatca
    23101 tcggagtgtc gaaatctaga cttcctcaga catgttcagt ttgccgcgca caattccatt
    23161 tattagaagc aaaaggttgt gcaggttgtc aaaaaccaat ccatttgaaa tacgaggaga
    23221 cgaaaaagaa agcaaatact agtttcgttt actgcaacga ctgtcaagaa tggaagaaga
    23281 aatatcctgc atatgatttt caacaccgcg ccttttttac gtacgatgaa aagatgcagg
    23341 cttggatcaa tcggtacaag tttatgggag atatccgatt agcagggact tttgcatata
    23401 aatggactca gataaaaaaa gattacccgg catatattta ttgtccaata cctttatctg
    23461 aaaaaaggtg gctagaaaga ggattcaatc aagtaagcga gatgttgaaa gcagcaaaaa
    23521 ttccttttca gatgctgctc aaaagagagc aacatctgct tgctcaagca aaaaacacgc
    23581 gaaaggaaag attggagatg cctcagcctt ttgagcttgc cgtacctctt tctaagatac
    23641 aacataaaaa aatattgatt attgacgatg tctatacaac aggacagacg ctttttcatg
    23701 cagcagattg cttgcagaac gcacagccga aaatcatccg aaccatttct ttggcaagat
    23761 gaatcagatg tggaaatttg atagcgattt aatttgcaaa taacctagag ttcgatataa
    23821 tagaattaag aagagggaaa gagtggtcct tctccttctt gattgtggta gacgaaaggg
    23881 gtaattttta tgtttagata taatgtacgc ggcgaaaata tcgaagtaac tgaagcgatt
    23941 cgcgactacg ttgagaaaaa agtaggcaaa ctagaacgct atttcagtga cgcacccgat
    24001 gcaacggcgc atgtgaactt aaaagtttat acagaaaaaa cagcaaaagt ggaagtaacg
    24061 atcccattac cttatcttgt attacgtgcc gaagaaactt ctccagattt atacgcaagt
    24121 atcgatttgg ttgttgacaa attggaacgc caaatccgca aattcaaaac aaaaattaat
    24181 cgtaaatccc gtgaaacggg catgaatact gcagatgctg ctgctttatt caatgaagag
    24241 gacgaaaacg aaaatgattc tgaattagat atcgtacgta cgaaacgcct ttcattgaaa
    24301 ccaatggata gtgaagaagc agtcttacaa atgaacatgc tgggacataa cttctttatt
    24361 tttgaagatg cagaaacaaa tggtacaagt atcgtttacc gccgtaaaga tggaaaatac
    24421 ggactgattg aaacagactg atcagcctta cttaaataac aacgaaaact tgcagcagct
    24481 actgacagct gccgcaagtt tttttgagtt tcctcctctt ttagatgaaa tatgaataaa
    24541 ttatgaagtt cttactttaa tacgcttaag atatttcaat tatctataac taatgataaa
    24601 atgaaaagta aagtctacat agtctggata atacacagaa ggaaaaggag attacacaac
    24661 gcatggccaa tttgattcgt tcacttatcg aaaacgataa gaaagaacta aaacgtttag
    24721 atagcattgc aaaaaaagtt gaagcatacg ctgaccaaat ggcagcatta actgatgaac
    24781 aattacaggc aaaaactgct gaattcaagg gcagatacca aaaaggagaa acattagatc
    24841 aattacttcc ggaagctttt gcagtcgtcc gagaagcagc aaaacgtgta ttaggtttat
    24901 atccatacca cgtacaattg atgggtggaa tcgtgcttca cgatggaaat atccctgaaa
    24961 tgcgtactgg tgaaggtaaa accttgaccg caacgatgcc agtatatcta aatgctctga
    25021 ctggcgaagg ggtacacgta gttaccgtca atgaatattt agcaacccgt gactcgacgg
    25081 aaatgggcga actttataat ttcttaggat taacagtggg gttgaatatc aactcaaaat
    25141 cttcggatga aaaacgcgag gcttataatt gtgatatcac ttatagtacg aataatgaat
    25201 taggatttga ctacttgaga gacaatatgg tcgtttaccg ccatcaaatg gtacaacgtc
    25261 cgctgaatta tgccattgtc gatgaagttg actcaatctt gattgatgaa gcgagaacgc
    25321 cgctgatcat ttcagggcaa gcagaaaaat ccacagcttt atatacaaga gcagacaact
    25381 tcgtgaaacg tttgaaagaa gaagaagatt ataaaatcga tgtacaatca aaaacaatca
    25441 gtttaaccga agcagggatc gaaaaagcag aagaaacatt cggtttggat aacttatacg
    25501 acattgaaaa tactgcattg acccatcact tggatcaagc attgagagcc aactatatca
    25561 tgcttttaga tatcgattat gttgtccaag acggaaaagt actgattgtt gaccagttca
    25621 ctggtcggat catggatgga cgccgttatt cagatggact tcaccaagca atcgaagcta
    25681 aagaaggcgt agagatcgaa gacgaaacga aaaccatggc gacgatcact ttccagaact
    25741 atttccgtat gtacaagaaa cttgcgggaa tgactggtac agctaaaaca gaagaagaag
    25801 aattcagaga aatttataac atccaagtat accagattcc gacaaatcgt ccggttatcc
    25861 gtgatgatcg tccagatttg ctttacccaa cattacaaag caagttccac gctgtcgtgc
    25921 aagatatcaa agatcgctac cgcaaaggtc aacctgtgtt agtcggaact gttgcggttg
    25981 aaacatctga gttattatca gatatgctga atcaagaacg tattcctcat gaagtgctga
    26041 atgcgaaaaa tcactttaaa gaagcagaga tcatcatgaa tgctggtcaa aaaggtgctg
    26101 tgacgattgc taccaacatg gctggtcggg gaacagatat caagttagga cttggggtaa
    26161 gagaacttgg tggtctagca gttatcggga cagaacgaca tgaatctcgt cgtatcgata
    26221 accagcttcg aggacgtgca ggacgtcaag gagaccctgg tatgtcacag ttctacttgt
    26281 cattggaaga tgatttgatg aaacgattcg gttcagaacg aatcaaagct ttcttagatc
    26341 ggatgaagat cggtgaggaa gatgctgtta tccaaagcaa gatgctgacg aaacaagtag
    26401 aatctgcaca aaaacgtgta gaaggaaata actatgacac acgtaaaaac gtcttacaat
    26461 acgatgatgt gatgcgtgaa caacgtgaag taatctataa acagcgacaa gaagtaatca
    26521 tggaagaaaa aagcttatcc aagccactaa tgaacatggt caaacggaca atcagtcgtg
    26581 tagtagatgc gcatacacaa ttagagaaag aaaattggaa tttggaaagt atcatcgatt
    26641 ttgccggcag tactcttgtc catgaagata gcattactct tgcagatatc gaaggaaaaa
    26701 cagctcagga gatcaaagag tatctaaacg aacgagcaca agaagtattt aataccaaag
    26761 cggctcaact aaatggtcca gagcaactgt tggaatttga aaaagtcgtg atcttgcgtg
    26821 ttgtggactc taaatggaca gaccatattg atgcaatgga tcaattacgt cagtcaatcg
    26881 gcttacgtgc ttatggacag aacaatccat tagtcgaata tcaaacagaa ggctataaga
    26941 tgtttgaaga tatggtaggc gccatcgagt atgaagtcac tcgaatcttt atgaaagctg
    27001 agatccgtca aaacgtacag cgtgaacagg tttctcaagg aacagcttct cactcagaag
    27061 acggagatgc caaagaaaat agtaatacta gcgcgaaaaa acagccaatc catgtgaata
    27121 aggtcggaag aaatgatcct tgtccttgtg gaagcgggaa aaaatataag aactgtcatg
    27181 gaaaaaatgc aaactaattt ctgacagcag gattggtgag acaaattctt tttttaagaa
    27241 ttcgtctcac cttctttctg ttatttgctg attctgttag actagatata atgaaaaaag
    27301 tatagtgaaa gaaggaaaaa acatggaaat atccgaaatt cgaaatctcc tgacagaaat
    27361 gcaggaaaag gtcacaagtt tcaggaggtc tctttgactt agatcagctg gaagaagaca
    27421 tcgcggaagc agaagcaaaa atggctgaac ctggtttttg ggatgacagc gaagccgcac
    27481 aaaaagtgat cagcgaaaat aacgctaata aagaaaccta tgaccaattc cattctcttg
    27541 ccgaagaact agaagaaatc gaattgatgg tggagatgct gcaagaagaa cctgatgcag
    27601 caatgcaaga agaagcagaa acacgtatca atgcattaga tgaaaaaatg aagacgtatg
    27661 aattatctat gctgttagac ggaccttatg atcgaaacaa tgcgatcgtc gaattgcatc
    27721 caggggctgg cggaacggag tcccaagact ggggcagcat gcttttacgt atgtatacac
    27781 gttgggcaga gcagcatggc tatcaagtag aaacactaga ttatcaagca ggtgatgaag
    27841 cagggatcaa gagtgtgacc ctgctgatta aaggacataa cgcttttggt tatctaaaat
    27901 cagaaaaagg tgttcatcgc cttgtacgga tttcgccttt tgactcagcg aaacgacgtc
    27961 atacttcttt ttgttcggta gatgtgatgc cagagcttga tgaaacgatc gatatagaaa
    28021 tcaatccaga tgatttgaaa atcgatacgt accgagcaag tggtgcaggt ggtcagcaca
    28081 tcaataaaac agaatctgct gtacggatca ctcacttgcc tacaggaaca gtagtagcta
    28141 gtcaggcaca acgctcacaa ttgaaaaacc gtgagcaagc gatggggatg ttaaaagcta
    28201 agctttatca attagaagtg gaaaagaaag aacaagaagc agctgcactt cgaggagaac
    28261 aaaaagaaat cggctggggt tcacagatcc gttcttatgt tttccatccc tattctatgg
    28321 taaaagacca tcggacaaac gtagagacag gaaatgtcca agcagttatg gacggtgacc
    28381 ttgatttatt tattgacggc tatttaaaaa tgcatctaca ataagaaccc attgttaaat
    28441 tgaaataaaa ttgtaagatt ttgcaagcat gttgaaacat tgccatgcta taatagctaa
    28501 agactgaaaa agatatggag agaacgccat gattgaaatg aaggatgtaa tgaagaagta
    28561 ctcaaatggt acgaccgcta tccgcaatct ctcgattgtc atcgaacaag gagagtttgt
    28621 ctatgtggtt ggtccgtctg gagccggtaa atctacattc atcaaattga tgtatcgaga
    28681 agaaaaagct tcaaaaggaa ctttgaacgt cgcaggtcat gatctaatag caatcaaaaa
    28741 ccgcgaagtt ccttatctac gcagagaaat cggcgtggtg ttccaagact ataaattatt
    28801 accaagaaaa acagtatatg aaaatgttgc gtacgctatg caagttatcg gcagaaaacc
    28861 tcgagatatc aaaaaacgag taatggaagt gctggacctt gttggtttga aacacaaagt
    28921 tcgagtcttt cctagcgaat tatccggtgg ggaacagcaa cgtgtctcta ttgctcgggc
    28981 aatcgtgaat acgcctaaag tacttatcgc ggatgaaccg acaggaaatc tggatccaga
    29041 aaactcatgg gaaatcatga aattattaga tcggatcaat gcccaaggaa ctactgtcgt
    29101 gatggctaca cataacagta cgatcgtaaa tacgattcgg caccgagtaa ttgctatcga
    29161 aaacggccgc attattcgag atcaagcgga aggggaatat ggatacgatg attagaacct
    29221 ttttccgcca tttattagaa agtatcaaga gtttgaaacg taacggctgg atgacagttg
    29281 cttcagtcag tgcggtaacg atcacgttga ctttagtcgg tgtattcatg gcagttatca
    29341 tgaatgcaac taaattagct caggatatag aaggaaacgt tgatgtatct gtattcgtag
    29401 atatcggtac aaaagacgaa gagatgaaaa agctagaaaa agaattgaat gacttagatc
    29461 atgttaaaaa agttgaattt tcaagcaaag aacaggaatt gaaaaaaatc caaaacgaaa
    29521 tgggtgattc ctggaaattg tttgaaggtg acaataaccc gttatatgat gtgtatgtag
    29581 taagtgctac tgatccttct tatacaaaac aagtagcaga agaagcagca gatttgaaga
    29641 atgtttctcg ttctgattac ggtggagctt cctcagatcg gattttccaa atcgctggtg
    29701 cagttcggac atggggatta gttgcagcgg cattgctgct gttcgtagcg atgtttctca
    29761 tttcgaacac gattcggatc acgattttgt ctagacagcg tgaaattcaa atcatgcgtt
    29821 tagtcggtgc aaaaaatgga tatattcgct ggccgttttt ccttgaaggt ggctggatcg
    29881 gtctgatcgg tgcaattctt ccaattttga tcatcacatt tggttatgca tggtttttcc
    29941 aattgatcaa tccatcatta ttacgttccc attattcatt gattcatcca caaaatatcg
    30001 tttggaaaat caatctttta atggtcggca ttggtgtgat cattggttca cttggctcga
    30061 tcatttctat gagacgtttc ttgaaattct aataccacaa tataaataaa atgagaaagt
    30121 tggacagttt cgttccagct ttcttttttt attggctagg agaatagaag gaagcggctt
    30181 ttttcccttt actttaaaaa acaacttggg tattcttaag ttggtagaag aagaggtgaa
    30241 gccgtgaaaa ttatcgttga caattttttt acgatccttt tgattatgaa tattttgctt
    30301 tcctttatca tcgtttttcg agaaagaaag gaaactgctc aaacatgggc atggctgctc
    30361 gttttgatgt tcattccagt tgtaggattt attttatata tttttttagg aagagggatt
    30421 tccaaagaga aaatctttga tttgaagatc caagggaaaa tcggtaagaa cctggaaatt
    30481 gaagaagaca aacaggcatt gatgcgaggg ctttatcctc atccgcctac tggacaagta
    30541 gatgtgaaac aattgatcta tatgctcacg gtatttgaaa gtacgctcta tacgactaaa
    30601 aatgaaatca ctttatttac agatggtcgt gaaaaattcg atgcgctgat tgaagatatc
    30661 aagcaggcag aagaccatat ccacttccag tattatattt atcgcagtga tgcattaggt
    30721 gaagaagttc gagatgcatt gacagaggcg gcaagacgag gggtaaaagt cagagtcctt
    30781 cttgatgcat ggggttccac acaagtttct tccagctttt ttcagaattt gaaaaaagca
    30841 ggcggagaag tcgcattttt ctttcctcta tttgttccgt atattaatcc tcggatcaat
    30901 tatcgcaatc atcgtaaaat cgtagttatt gatggggaaa tcggatatac aggtgggttc
    30961 aatgttggaa atgaatatct aggattggta aagaaatttg gttattggcg agacaatcat
    31021 ttgcgtattt acggagaagc ggtgtatacc ttgcaaaacc gttttttgat ggactggaat
    31081 tctcagcata caaaagaagt aggctattct cccaaatttt ttccgtccat tcacactact
    31141 ggtgatatag ctgttcaaat cgtgacaagc ggaccagata ccgaacatga acaaatcaaa
    31201 atgacttatt tgaaaatgat ttcgttagcc aaacgggaga tattgatcca aacgccttat
    31261 tacataccgg atggatcgat tcatgaagca ctgaagttgg ctcttctttc aggcgtaaaa
    31321 gttcatatcc aaatacctaa caaaccagat catcttctcg tttactgggc aacgtattct
    31381 ttcgctgcag agctgatcga gtatggagca aggatcgaaa catatgagaa tggatttatt
    31441 catgcaaaga cgatgattat cgatggtggc attgtttcag ttggctcagc aaatatcgat
    31501 gttcgttcat ttcgcttgga ctttgaggtc aatacactgg tttatgatga acgaatggct
    31561 tcacgtgtaa gaaaagcttt ttttgaagac tcgaaaattt ctacgcatct cacaaaagaa
    31621 atgtatgaaa accgaggaat aatcatcaag atgaaagaag gattagcaag attgatttcg
    31681 ccgctccttt aaaacataaa aaggacatga gaaccatcaa cagatggaga tctcgtgtcc
    31741 ttttttaacg gggccatttc tgagagtacg tcagaaatgg agaaagcgtc tcgcatgagc
    31801 gaaattaact ttggggtatc tcttgttttt tggggaaagc aagaaataag ctaatatatg
    31861 ctattaaaag agacacattt caaagccagt atag
//
LOCUS       DAFCNS010000032        31337 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFCNS010000032 DAFCNS010000000
VERSION     DAFCNS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.032"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            316..1185
                     /locus_tag="LNE23_001361"
     CDS             316..1185
                     /locus_tag="LNE23_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBK6931222.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1394..2419
                     /locus_tag="LNE23_001362"
     CDS             1394..2419
                     /locus_tag="LNE23_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK6931223.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2458..3408
                     /gene="manA"
                     /locus_tag="LNE23_001363"
     CDS             2458..3408
                     /gene="manA"
                     /locus_tag="LNE23_001363"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBK6931224.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3502..5205
                     /locus_tag="LNE23_001364"
     CDS             3502..5205
                     /locus_tag="LNE23_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6931225.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5207..7240
                     /locus_tag="LNE23_001365"
     CDS             5207..7240
                     /locus_tag="LNE23_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6931226.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7251..7691
                     /locus_tag="LNE23_001366"
     CDS             7251..7691
                     /locus_tag="LNE23_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6931227.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7891..9018)
                     /locus_tag="LNE23_001367"
     CDS             complement(7891..9018)
                     /locus_tag="LNE23_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931228.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9021..9698)
                     /locus_tag="LNE23_001368"
     CDS             complement(9021..9698)
                     /locus_tag="LNE23_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931229.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9962..11728
                     /locus_tag="LNE23_001369"
     CDS             9962..11728
                     /locus_tag="LNE23_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBK6931230.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11743..12180
                     /locus_tag="LNE23_001370"
     CDS             11743..12180
                     /locus_tag="LNE23_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6931231.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12193..13344
                     /locus_tag="LNE23_001371"
     CDS             12193..13344
                     /locus_tag="LNE23_001371"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBK6931232.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13406..14104)
                     /locus_tag="LNE23_001372"
     CDS             complement(13406..14104)
                     /locus_tag="LNE23_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBK6931233.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14097..15449)
                     /locus_tag="LNE23_001373"
     CDS             complement(14097..15449)
                     /locus_tag="LNE23_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBK6931234.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15672..17072)
                     /locus_tag="LNE23_001374"
     CDS             complement(15672..17072)
                     /locus_tag="LNE23_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBK6931235.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17233..17970
                     /locus_tag="LNE23_001375"
     CDS             17233..17970
                     /locus_tag="LNE23_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBK6931236.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18095..18574
                     /locus_tag="LNE23_001376"
     CDS             18095..18574
                     /locus_tag="LNE23_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBK6931237.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18571..19338
                     /locus_tag="LNE23_001377"
     CDS             18571..19338
                     /locus_tag="LNE23_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931238.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19568..20137
                     /gene="lepB"
                     /locus_tag="LNE23_001378"
     CDS             19568..20137
                     /gene="lepB"
                     /locus_tag="LNE23_001378"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK6931239.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20241..20843)
                     /locus_tag="LNE23_001379"
     CDS             complement(20241..20843)
                     /locus_tag="LNE23_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6931240.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20949..21418
                     /locus_tag="LNE23_001380"
                     /pseudo
     CDS             20949..21418
                     /locus_tag="LNE23_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21524..22108
                     /locus_tag="LNE23_001381"
     CDS             21524..22108
                     /locus_tag="LNE23_001381"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBK6931241.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22111..23184
                     /gene="prfA"
                     /locus_tag="LNE23_001382"
     CDS             22111..23184
                     /gene="prfA"
                     /locus_tag="LNE23_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBK6931242.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23177..24016
                     /gene="prmC"
                     /locus_tag="LNE23_001383"
     CDS             23177..24016
                     /gene="prmC"
                     /locus_tag="LNE23_001383"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBK6931243.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            24009..24188
                     /locus_tag="LNE23_001384"
     CDS             24009..24188
                     /locus_tag="LNE23_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931244.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24210..25235
                     /locus_tag="LNE23_001385"
     CDS             24210..25235
                     /locus_tag="LNE23_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBK6931245.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25410..26654
                     /locus_tag="LNE23_001386"
     CDS             25410..26654
                     /locus_tag="LNE23_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBK6931246.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26807..27754
                     /locus_tag="LNE23_001387"
     CDS             26807..27754
                     /locus_tag="LNE23_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6931247.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27888..28517
                     /gene="upp"
                     /locus_tag="LNE23_001388"
     CDS             27888..28517
                     /gene="upp"
                     /locus_tag="LNE23_001388"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBK6931248.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28656..28898)
                     /locus_tag="LNE23_001389"
     CDS             complement(28656..28898)
                     /locus_tag="LNE23_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931249.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            29019..29091
                     /locus_tag="LNE23_001390"
     tRNA            29019..29091
                     /locus_tag="LNE23_001390"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29052..29054,aa:Thr,seq:cgt)
     gene            complement(29210..29848)
                     /locus_tag="LNE23_001391"
     CDS             complement(29210..29848)
                     /locus_tag="LNE23_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK6931250.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29971..30603)
                     /locus_tag="LNE23_001392"
     CDS             complement(29971..30603)
                     /locus_tag="LNE23_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK6931251.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30954..31253
                     /locus_tag="LNE23_001393"
     CDS             30954..31253
                     /locus_tag="LNE23_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6931252.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10681 a         5483 c         6311 g         8862 t
ORIGIN      
        1 atttttctta ctactcgaaa ctaagcgact ttttcacaac ctcttcctta aacggtgttc
       61 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aataggagga gctatttttg
      121 tttggctgtt cttattactt gatgcagaac agctttttca caacctcttt ttataagagt
      181 ttttactcat taatttacaa aaaacaagca aaatgcgaat tttctcacat tttttttaac
      241 aaaatcaaag ttattgcttg aaagaagaag aatccatcgt taaaataaag cttgacgtag
      301 aggggagcaa gaaatatgaa agattttcaa ttgggggctg ccacctattt aaaaagtgta
      361 gccattcggg tgaaagaccg agataaaatg attgattttt acaagaacgt tgttggcttt
      421 gatttgaaaa gagaagaaaa cgaattagcc attttaggaa cagaagaagc tggaagtgaa
      481 atgttgctgc tggaagaaag tcctagagcg aatgatcaca tgggggtgat caaaaagctg
      541 aaccgttttt cattgattat ccctagcaca gaagaaatgg cagatatcct ttgtcgtatc
      601 cgtaaaatga attatccaat caaagaagct ttagaagatc atggacgtat ggggattttg
      661 ctcgtagacc cagaagagaa tgaattggaa atttaccatg aagagaataa gaacgaccaa
      721 agagaaattc cagaaacaat ggatcaagaa gcattattaa gaaaatctga agaaaaattc
      781 agtaaactat caacaggttc ttattttgaa aaaatccatt taaatgttac taacttaaca
      841 aaagaacgcc aatttcttaa tcatgtactt ggtttgaagg tacataatga agaagagggc
      901 atccatgtat tgaatgaagg agatttccat gttggtttga cagaagctag cggaggcgca
      961 attgatttgc ctacagatga agtgattgga cttgattttc tgaaattcat gatcgatact
     1021 gaaagcatgg aaggactgat tgctcacttg cagcaactat cagttgattt cttcttagac
     1081 aaaaagcaca cagtactcac aatctatgat gctataggaa tcgaatggtg gttcgtcagt
     1141 gagccaaaag aaaagaataa aactgacatt cgatgcgacg cttaactttt tctttcttaa
     1201 cgattgtcgc atgtctcgtc attttatgaa cggtcatact atcgcttatt ctagagatag
     1261 aaagagaaaa tagtataccg ggggaaactg cgcggttttt cgcgcagttt ttttgtgtaa
     1321 aaaaggttga ttcctcatag attaagcttt aagcttggac tgtaagaatc tgatagaaaa
     1381 gcaggagaaa aagatgtatc cacacacgaa acagcttatt ttgaaaaaga tggaagaagg
     1441 attttatcca ggtgtagcag tcgccttcat aaaagaaaat caagtagaaa catttacagc
     1501 cggctttgcc caagtcgttc ctgaaaagca gctgatgaca gaagatctgt tgtttgatgc
     1561 agcctcactg acgaaagtcg ttgcaaccac ttcattagtt ttacgtctat tagaagatgg
     1621 aaagatagaa ctcgatcaac cgcttcataa atatcttcct gaatttgaaa atcagacaat
     1681 cactttgcgt catttactga cacatacttc tgatatcaaa acatggatac caaacagaga
     1741 taagctatct caggaagaat tgatcgccgc ttatttgaag ctagaaccag gtgagcaact
     1801 aggaaaagaa gtaaaatata cagatgccgg tacgattctt cttgggttga tgctagagga
     1861 aatttaccaa gaaactgcag tggaaatctt tcaagaacaa gtattggaac cattaggaat
     1921 gatgaacagc ttatttcttc cttttccttc caaaaaaatc gttcccacag aacaactgcc
     1981 ttcgggtgag gttttgcgtg gacaaactca tgatccgaaa gcaagagttt tggctgaaca
     2041 tgcaggtaac gcgggtctct ttattgattt gaaagacagc attacttttg tccaaatgta
     2101 tttgaacaag ggaaaaatcc gtaatggtcg ttttttagaa gaagagacga ttgattcttt
     2161 actgcaagat caaacaccga aaaaaaatgg ctatcgttct ttaggatggg acttaaaaaa
     2221 ggatgtagaa gacggtcatg cgattctttt tcatactggt tatacaggta cgtttttctt
     2281 gattgatata aaaacacaag aagcgttgat atttttgtca aatcgcgtac atcctgtcga
     2341 tcatcgtgcg gagtatctca tatgtcgaga tgaaattgtt gaaaactttt tgaaagagaa
     2401 agcggtatta ccgaaataat gctataatag ctatgaaaaa tgagaggagt ttgaattatg
     2461 caggaaccta tgtttttaaa accagttttt caagaaaaaa tttggggagg aagtcgttta
     2521 cgttcagtct ttggttttga tatccctaac gataaaatcg gagaagattg ggcgatcagt
     2581 gcgcatcctc atggtgtcag tgtagtagaa aatggcgaat tcaaagggaa aagattagat
     2641 gaactatggt cagaacacaa ggaattgttt ggccatcctt cagaaccagt gtttccatta
     2701 ttgatcaaga tcttagacgc agaagatgat ctatctgtac aagtacatcc agatgatgcc
     2761 tatgggatga aacatgaagg tgaattagga aaaactgaat gctggtatat catcgatgct
     2821 gaacctggtg cagaaatcat ctatggtcat catgcaaaga caagagaaga actagctgag
     2881 atgatcaaag atgggcgctg ggatgacctt ttgaagaaag taccagttaa aaaaggtgat
     2941 ttcttttatg taccaagcgg aacgatccat gctattggta aaggaatcat gattttagaa
     3001 acacagcaaa gttctgacac gacttaccgc gtgtatgact acgacagaaa agatgcgcaa
     3061 ggacaaacaa gagagctgca tatccaacaa tcgatcgatg taacaactgt ccctgcgaaa
     3121 acaccagaat tacaaatcaa agaagtccga aaagggaatt catcgatcgt cacttattta
     3181 gaaactgagt ttttcaatgt atatgagtgg gatattaaag gaatcacatc tttcaaaaaa
     3241 caagcccctt atacattgat gacggtaatc gaaggagcag gagaccttgt cgttgacgga
     3301 aaaacatatc cattagaaaa aggaacaagc tgtattattc caaatggcgt ttctgagtgg
     3361 gcggtccaag gtgaactagc aatcattgcg tctgaaccag gcaaatagtt cccagatagc
     3421 tgaaagcagc agacggtttt tcgtttgctg ctttttctac ataaaatccg tccatcaata
     3481 aatagaagtc tggtgaaaaa gatggaatac aaatggaaag caaacaacag cagtcgcgtg
     3541 tctatgaaac aactgagaaa agctggacat cacttaaatg aaatgatcat gccaaatcta
     3601 agtctgttta tcgcttgggg aatcttgtca ttagcaacaa aaggtgtgtc tggcgatttg
     3661 cacaccacat tagaagaggt atgccattgg atgatccagc tgcttttacc catcttgatc
     3721 agttattcag gcgggaaatc ggtaggggga caaaaagggg cagtcgccgg tgcaattgct
     3781 tctttagggt tgatcgctcg ttccaatgct ccacaaatcg taggagcgat gattatcggg
     3841 ccgcttgccg gcatcgtata tgaacaattt gtttctagat tccatgtgaa attcaaggct
     3901 ggatatgaaa tgcttgtcag caacctacta gtaggactga ctggtagcgt tatttgtata
     3961 attggaatcg tttttattga accagtttta aatagtgttc acttgttgct cgcctccgtt
     4021 gtgtcttggc tagtcacaat gaatctgtta ccacttgtca gtctgatctt agaacctcta
     4081 aaagtactat ttttcaataa tgcaatcaat catggtgtat tgacaccttt aggaatcgaa
     4141 ttctcacaaa cagtcgggta ctcttcctta tttttgatgg aggcaaatcc tggacctggt
     4201 ttagggatct tactttctat cctttgtttt gcagaaaaac aggaaaaagt aaatgcgagt
     4261 ggagcattga tgatccatgc gattggcggg attcacgaaa tctattttcc atttgtcttg
     4321 ctgcgaccgt atctattttt agctgtaatg gctggtgggg caagcggcac gttgatattt
     4381 caatggttag atacgggatt agctgcacct gtatcgcctg ggtcactact cgtgattttt
     4441 gccaatgcgc cttatccaca actagctggg gtaagtatag ggatagcaat cagtacattt
     4501 gtgaccttca tttgtgcagc gatactattg aaattaccaa ataaagaaaa agagaacaaa
     4561 acggtcaaag aaaggggaga aatcttggaa ccatctatca ttcattccgt catttttgct
     4621 tgcgactcag gtatgggatc aagtgctatg ggagccgccc tgctgaaaaa gaaattagca
     4681 gcagaaaaaa tcgatctttc tgtcggatac agctctatct atgaattgaa taaccaagta
     4741 aatcagctgg ttatcgtgca acatgaacta gcgggtcttg caagagaaaa agtacctcaa
     4801 agtcaaatct atgtactgga tcactttttg gaaacagata tcttgctgtc acgaataaaa
     4861 gaagcgatcc aatttccaaa gcgggaagaa aaaaaagaga tagcagatat cacagaacaa
     4921 aaaacgtcgc ctgctaatat tgtatttttg tataaaaaca acagacgcgg ctcacaaact
     4981 atgggcatga cgattttaag tcaattagca aaaaaagcga aaaaacagtt tgttatctca
     5041 aaagaatcaa tagaaaaaat caaaatccaa gaagaaacga tttatatcgc acaaagagaa
     5101 ctagctgtaa ctgaacagct agaagaaaag atccctcggc tagttctagt tgaacattgg
     5161 gtagctacga atgaatacga aaaactcgtg aagggagaaa tataagatgt atttatcgcc
     5221 cagagaaaaa caattattag cagaatttct cagtagcccg tcacctgtat ctatccaaaa
     5281 aatgatgaat ttattgaagg tcagtaaacg gactatttac cgcgaattag ataatcttga
     5341 agtctcattg aaatcggtaa atgcttcttt gaaaaaagca gctagaggct catttgtcat
     5401 tgaggcatca gaagaagtat tagagcaatt aaaagaaaat acagatggga aaagtaatga
     5461 cttatccact gctaaacgac aacacgcgat tttagccgag ttgttactga cgacagagcc
     5521 gttgagtctt gctcatttca tggaatcata tcgcatcagt aataccactt tttatgcaga
     5581 tatcaaacag ttggaagaaa gtattcgcca actgcctttg gaaatcattc ggaaccaggg
     5641 atacgagatc gaaggaccgg aaaaataccg ccggcttctg acagcgaatg tattagaatt
     5701 agaaatcaat gaatatgaac tttttcatag tatttctttc gatgcatcac tgaattattt
     5761 ttttcgattc gttgatccgc aacatctatc tcttgcacgc aaagtaatcg gggaagaatt
     5821 gatccaaaaa aagaccaatc taagtgatag aaagcttgaa catttggttt tgatgctgac
     5881 attgacgatg gagcgtgtga cgaaaaatca tttactgaca aatgagacat ataatagtct
     5941 ggcaaacaaa gaattactac agacagctaa acgattgttt tcacggatcg cttctgagac
     6001 aaaacaactt tatccagtaa atgagatcgt cttttttgca ggattattaa atgacttcac
     6061 gaactcattt gatcagaatt tctttgaaga gacgttcgat acacagcttg cttatttagt
     6121 aaaacaactg attcaagaag taagtgaaga gacgaatgtc catttttatg aagatgaaac
     6181 tttgtacaaa atgctgttga ctcatttatc tggtgtgttt tcaagagccg ttttacaaga
     6241 agagacactg accaatccga ttttagaaaa aatcatggtt caatacgagg aagttgcgaa
     6301 tgcaatccgt aaagcagtag agcggatttt tcaagaaaaa aaattatccg aagaagagat
     6361 cgcttatatg gtccttcatt ttgcgaattc attggaacga agtccgaaag aaagcaatat
     6421 cagtattgca ggattttcgc caagtggatt agcttctact agcatgttgg aactacgatt
     6481 gaaaaggtat tttcccttca tccaacaaat tcatttctat cggattgctg atttaaaaaa
     6541 aatcgattta caagaagaat atgatgttgt tatttctaca tctattttac caggatacgg
     6601 tggaaaatat attttagtct ccccgctact gctagaagat gaagtcaagc agctaaaaga
     6661 agctttttcg catattgaga agaaaaaaag gcaaaaaatt acgtataaca gcaataacga
     6721 gtggatgata gaagatactt atgaggaaac aatgcatttc atggatcaaa tcaatcaatt
     6781 actcggtcgt ttttttatcc aagaactgga taatcctgag acgatttccg aaacgattcg
     6841 cagcttattt ccttattttc ctgaaggact agtaacggat gaagaggtgg ttcatcgccg
     6901 gctgcttaag cgatatgaac aagcacctat cggtattcct catacaaata tggggttatt
     6961 ccatacatcc agctcattga tttctaaacc gatcttttgt attttcaatt tgaaacatcc
     7021 gttgaccatt gaagggatgg atagacagcc agtcgatttg tcccgtatgt tagttatgct
     7081 ggcgccttcg ccaatccaag aaaaagacgg gattctttta ggaaagatca gtggagcaat
     7141 catcatgaat gacttgaaca ccgagatctt ccgctcagga aataaagaaa tcgtttatca
     7201 gttgttagcg aaaatcctga ttgaagaaat aaagaaatga aggtgaaaag atgatacagc
     7261 taccagcaga aaaaatattg ctcgatcaat cctatcaaaa ttggaaagaa gcgttgaccg
     7321 agatcggcca gtggatgacc caacaagggg gaatcaaacc ggcatatatc caatcaatga
     7381 tcgaaagaca agaaaaagcg agtgtctata tcgggaattt cgtagccttg ccacatggga
     7441 ctgacaaaga tgaagaagta gtagaagaag gaatctattt agtccaagtg cctgatggtg
     7501 tggactttgg aacaaaagag gaaccaaaaa tcgcgacact cttatttgta gtggctttaa
     7561 aaaaagaaca tcagttagcg gtgctccaag atttagcctt tttctgttca gacatcgacc
     7621 aagtaatggc gctcagtgat gcggaaacca ttgaagaagt ccaaatgatt tttgaacatg
     7681 gacatatata accaatgttc acagtaaaaa aagctgcctt gcatcaagta ataacttcaa
     7741 ccaagataaa aataagagga gctatttttg ttgggaggtt cttattactg cggatgcaag
     7801 cttttgaaaa ctatttattc tagaaaaccg tttatttgaa ataggggacg gtgacaaggt
     7861 ttttgtcacc gcccctatta tttttgtgtt ttaatagctc ttttttatat agatagtgcc
     7921 catacctatg aataataaaa cgatactcca tataccaagt gtcaagatac cattcatgaa
     7981 agtaacctgt tctgaagcta gtggcgagaa aatatctcct cctatgatca cattttgaaa
     8041 atcaacataa gaagttggca agtaatgact gattgattct atccatccaa tcgaagaaaa
     8101 caagcgatac tctgctaaga aaagacaaag aagtgtacct aaaagattga ctagagaatg
     8161 ttcaaataaa gcagatagaa gaaaactgat tgtacttaaa aagagcaccc acaacaacag
     8221 gaacagaaag ctctggaaaa gatactcata catcggcagg attcgtatct cctgaccaat
     8281 gatcgttcct acaggataac ggaagtctcc caaaccattt ttgattatta ctagtataga
     8341 gacgatcaaa aatggcatca cgagacttag catagctgca ccataataaa tggtaaaacg
     8401 gatgctatgt ttctttacca tactatccgg taatagattg actagagaga ttacctgctt
     8461 tcttttttca taagagatga tggaagaaag aaacaaggcg gttatacata aaatcaaaaa
     8521 actagaaatc attcctgaaa aaatattttc gtagtaattt gctaaagaga gatgcgctgg
     8581 caagtcaaaa atagaatatc tttggatctg atgttctttc atataacgga gcaactcgac
     8641 acgtttttgt tgttcaatga ttggaattcc ttgtctttga ccagaaatca gtcgatccag
     8701 tatatctttt tcataatgat aagttgcttc gaccatttgc tggacattcc ctgtgttttt
     8761 tgcatgtaag attgcttcga tcagtttgtt gcttttctct aaactttcca caaattcttt
     8821 ctcattctca tcttctttca acgtttcaat caaacgttgc atctcttcat agttatgatt
     8881 cagttcatcg aattctgtat aagcaagttt atgatcaaaa aaaagtggga atgcaaagag
     8941 cacgaccaac aatagaggaa ataaaaagtt tactttgttt tttagtaaga gattgcgttc
     9001 aaatgtaagt aaagacatag ttacacctca tataatttac gataaatagc taaaatctct
     9061 tccatatttg aggattctgt gacgatttct ccatttttta gaaaacatgc tttagaacaa
     9121 atcgattgga tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc ctctttctgc
     9181 cattgtctga tgaggctatt caagatatca atacttcctg ggtctaatcc gttcaacggt
     9241 tcatcgaata acatgacaga tgaatcactg atcaagtaag cagcaagcaa gacatgttgt
     9301 ttcattccta gagacatttt tccaatcggc aaagtaaggt actcagtcat cttcaatcgt
     9361 tggaaaacag tttggcattc tactttgctc tgccaatagt atttgatcaa agtcaaataa
     9421 tcaacagccg atagatagtc ggataactga ttcgttgatt ctagaaaaaa gaaagaccgg
     9481 cgataatctg tataagcagt aggagagatg ttgtgaaggg acaattgatc ataagttgtt
     9541 ggcagcagtt gggcaatgcc attcagcaaa gttgttttgc caaatccgtt cggggcaatc
     9601 aaagcagcta cttctcctga ttctactaaa agattcaatt gtcgaatcac acgtttttta
     9661 ccacgtttca tgttcatgtt tttgatcagt aatgtcatag ataggcctcc aatataaaat
     9721 ttaataacat aatagaatgg aaaacatcta aattaaatgc taacaaaagt tattgtttgc
     9781 atattataaa aaaattaaaa taacagtttt atttaaaatt gaagttttct gataccgaaa
     9841 tctttttatt ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc aaagtacgac
     9901 tatttctctt tttagtaagc ggatacaatt agagtatcaa aaggagagag ggagagacaa
     9961 aatggaacgg acgattccca aaaacaacat ttcgctaaaa gcgagggtac aaaaattagg
    10021 cagtacttta tcaagcatgg tcatgccaaa tattggagca ctgattgcct ggggtgtatt
    10081 aacggctcta tttatcccag atggttattt accaaatgag gctttcgcta ccatggtaag
    10141 cccaatgcta acttatctga ttcctttatt gatcggttat actggcggaa aagtaattgc
    10201 tggtgaccgc ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg tcggaacaga
    10261 tattccaatg atgctaggcg cgatgatcat gggacctctt ggtggatatg ccatcaagaa
    10321 atttgatcag ctattccaaa aacggatcaa atccggtttt gaaatgctag tcaataattt
    10381 ttcagcaggt cttatcggtt ttggattagc gttattaggt ttttctgcga ttggtccagt
    10441 tgtcgatgca ttgactcaag caatggcaaa aggtgtagag atcattttat ctgcccattt
    10501 gattccattg acaagtattt ttattgaacc agcaaaaatc ttatttttga ataatgcaat
    10561 caatcatggg attttgacac cattgggaac tgaacaagta gcagaggctg gaaaatctat
    10621 tctattcttg cttgaagcaa atcctggtcc aggacttggc gtattacttg cttttacttt
    10681 gtttggcaaa ggagcagcaa aatcttcagc accaggagcc atgatcattc actttttagg
    10741 cgggatccat gaaatctatt ttccttacgt tatgatgaaa ccgttattat tcttatcagt
    10801 tataggcgga ggaatgtcag gaagtttcat tttccaattg atggatgctg gactaagagc
    10861 gccagcttct ccagggtcga tcattgcgat tctagcgatg acaccgttca attcttacct
    10921 tcctgttatt ttgggcgtga ttgttgccgc agctgtttcg tttggtattt ctgcagtaat
    10981 tttgaaagcg gatgcgaaag atacaaatga agacttcgaa aaatctgttc aagagacaaa
    11041 ggcagctaag caagcttcca aaggtgccca aggtattcct caaggagcta cattctctgg
    11101 cgtacaaaaa atcatttttg cttgtgatgc aggaatggga tcaagtgcga tgggcgcttc
    11161 gatcttgaga aaaaaagtac aggaagcaga cttgcctcaa acggtgacca atcaagcaat
    11221 cagccaactg acagatgact cgaatacatt gatcgttact caggtcgaac tacaagaaag
    11281 agcaaaacaa aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt tgaattcacc
    11341 tcgatatgat gaaatcattc aagaattaag cagcaagcag aaaacatcag tttctgaaat
    11401 tgagaatgag ccagcagaag agtttctttc agcaaacatc aaacaaatca aagagattct
    11461 acttgtccac gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc tcaaagatat
    11521 attagacaag aaccatttaa ctatcccgtt aagaaaagtc aatatccaag atttaaaaga
    11581 tgaacctgat tcactgatcg tagccaaaga aagtttagcg gaaaaagtac agatggctgc
    11641 acctcaatcc attcatttat cagtcaacag catggtcaat ccgcaaaaat acgaatcgat
    11701 tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag tttgccattc
    11761 gacatgatcg agctcaatcg atcttttaaa accaaagaag aagcgatccg ctattgcggt
    11821 cagatgcttg tagaagcagg gtgcgttgat gaggaatata tcgatgccat ggtagataga
    11881 gacaaaatgt tatctgttta tatggggaat tttatcgcga ttcctcatgg aacagatgaa
    11941 gcaaaaaaat atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga tggtgtagat
    12001 tttggtaccg aagaagaaga aaagattgcg actgttttgt tcggtattgc aggagtcgga
    12061 gatgagcatt tgcaacttgt acagcaaatc gcactttatt gcagtgatat ggacaatgtt
    12121 gtccagctag cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc aattgcttaa
    12181 ggagagagaa aaatgagagc agtacatttt ggtgcaggaa atatcggtcg aggatttatt
    12241 ggagaggttt tggcagctaa tggatttgag attactttcg tagatgtgaa tcacacgatc
    12301 attgatgctt taaatgaaag aaaagaatac gaaatcgaat tagcagatga atcaaaagaa
    12361 aaaatcactg taaaaaatgt caaaggaatc aacaatcaaa caaatccaga aaaagtaatc
    12421 caagaaatcg ctgaagcaga tttagttact accgccattg gcccgaatat cttacctttt
    12481 attgctgagt tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag cacaacatct
    12541 ttagatatca ttgcctgtga aaatatgatt ggcggttcag catttctaga aaaagaagtg
    12601 tataaatatc ttccggaaac atcatttact gacaaatata tcggctttcc aaatgcagca
    12661 gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt tattcgtcca agtggaacct
    12721 ttcaaagaat gggtcattga cgatagccaa agaaaaaata aaaaaatcca attaaaaggg
    12781 gttttatatg tagatgatct agaaccatat atcgaacgca aactatttag tgtcaatact
    12841 ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt accagaccat tgatgaagca
    12901 atgcaagatg cgttagttgt ggcacaatta aaatcagttt tgcaggaaac aggaagtttg
    12961 ctgattgcca aatgggggtt tgatgcagag cagcatcatg cgtatattga aaaaatcatt
    13021 catcgcttcc aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg gacaccatta
    13081 cgtaagttag gttatcaaga aagatttaca cgtccagtga gagaactaca agaacataac
    13141 ttgacttgcc cacatttgac ggcaacaatg gggattattt ttaattacta tgatcctgaa
    13201 gatgaacaaa gccgtcaatt gcatgaaatg aagatccatg aaaacttgga acaattgatt
    13261 caagaagtca caggaatcaa tgatccgaaa acaatcggga atatcaagca aaacgtgaac
    13321 cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg acgaaatttt
    13381 tgtcacattc ccctttttcg aataactagg ctgtttgtcc ttcaagatag ggcagatcca
    13441 attctttacc gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc aggttctcat
    13501 ttcttgcaag gataggtcca tggaaataag aaccgaagac atttcggtaa atgacacctt
    13561 cagattggtc ttcaccatta tttccttgac cttgtaccac tttccccaaa ggtcgttcac
    13621 cttcacctaa gaaagtccga ccattgtggt tttcaaatcc ataataagtt tcattgaatt
    13681 cttcgttatg gataacgata tcaccgataa accggttatt ttcctgactt aacgtgtagt
    13741 gatccagtgc actgattcct tggatctttt ctccattagc tcccatgtag tagtgaccta
    13801 gcaattggta gccaccacaa atggctaaca tgacgccatc attttcgata tagttgatca
    13861 aagaatcttt ctttttctgg atgtcttttg aaatgattaa ttgctcaaag tcttgaccac
    13921 cgccaaagaa aaccaagtca tatttatcag catcaaaagg ttcatgtata ctaacgatct
    13981 ccgtatggca agaaaccccc attttttctg ctatatattt caacatcaaa aggtttccgt
    14041 tatcaccata tgtgttcatc aaatttccat aaaggtgggc gatattgagt tcataattag
    14101 ccacggttca ttcctccttt gataaatcct tgagcagcta gttctttacg aagctgcaat
    14161 acagcagtgt aagtagctaa aatatagaca tgctcagttg gcaattcttt gattttttcg
    14221 attacttctg gtaattcttt tgtttctgtc aatttatttt ctggaatccc ggcaactttt
    14281 agacgtaaag tcatatctgt atgacggtct cctccagaaa taacagctgg gatatccata
    14341 tcggcaaagt tttcaaaatt accatcccaa atccagctga catcgattcc gtctgcgtaa
    14401 ttggcattta ataaagccac taaagaaaat ggataaggag ctagaccaat catatcgatt
    14461 acttgattga tacctacagg atttttgaca agtactaatg tacatttttt cccattgata
    14521 ttgatgactt cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg tttgatttta
    14581 tcaggatcga cattgaagta ctctgccact gcagtagctg ctaaagcatt gtagacatta
    14641 tacataccgc caacttcgat tccgtattca tgaccatcga tcacgaaatc tgctgacaca
    14701 ttgtccatcc gtaccatatc agttaatcga taatccaatt caggacgttt gaagtcacaa
    14761 tttggacagt aatatttccc taagtttgca taagtgatca ttttataatg caagatgtga
    14821 tgacaatttg gacataagac accgtctgta ttgtaatgag ccatttgttc acgatcttct
    14881 tcatggtcaa agccataata ttttctagga ttgactgttt cgatagaatt gaagattggt
    14941 gaatcaccgt tactgataat cgttgcattt ggtgactttg cggcaccgtc tacaatcatt
    15001 ttataggtcg tatagatttc tccataccga tccatttgat cacggaaaat atttgtaaat
    15061 aagaacagtt ctggtttcat atattctgtg actttgctca aacttgcttc atctatttct
    15121 aggacagcaa actttttctg tccttttttt ggttttgcct gcaagaaagt cgatacaatt
    15181 ccctgtacca tattcgcacc agtagggttg gttagaactt catcgaattg ctggcgcaaa
    15241 atattgactg ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac aacgacgact
    15301 tggtagtctt ttgccaaact atctagtata tgtggatcga ctttcagcgc tagtttacct
    15361 ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc attgcgacgt ttttccaact
    15421 gcaatcgcaa aatgactgcg tattcccatt atgtaacctc caaaaacgat ttgctgacga
    15481 ataaattcaa ctaatcatac catatgttga aaaaaaggtt aaattgcttt tcttattctt
    15541 aagaaaaggt tacggctgaa agtgcatgaa atcggacaaa atcgcatgat ttaacaaaaa
    15601 aacagcttga aacaaaatga ttgtttcaag ctgctgtgat tttattattc cctcttacct
    15661 atattcccat tttaaccgat aataattttc tcgcttggat attcatagcc tagatctcgt
    15721 gtttcttttg gcacgaataa catcaatgta tatagcattc cgatccgccc aataaacatc
    15781 agtgtagcaa tcgtgatttt gcccacaaca gacaagtctc cggtaatccc taaagataaa
    15841 ccagttgttc caaacgcaga agttacttcg atgatgatcg agatcagcgt ttgttcttct
    15901 gtagcagata aaaaaacgat acaaaagaaa cacataccaa gagaaagcat aaagacaacg
    15961 actgattttc gcacatcatc ttgatcgatt ctccgtccaa aaatattgat gttatcttca
    16021 cttttgagga aggaatatag atacaaaccg atgattgcta cagtagttgt tcgaatcccg
    16081 ccgcctacag aactaggact gcatccgata aacatcagta aagaaaaaat gatcagtgtt
    16141 gtgatttgaa aatcacccaa atcgtgaatc tgcagaccag cattacgagt cgtaatcgaa
    16201 taaaacatcg aattgatcca tttgacactc atattggaat cttggaataa atggtccttt
    16261 tccaatagat aaatcaagac agttcccgaa acaaacaaga taacaaaagc taacacagct
    16321 aatttggtaa acaaggaaaa acgaaacgga agcttcgcat tggaacgttt gtataatagc
    16381 cattcccggc actccatcaa tacaggaaaa ccaattcctc cgacaaaaat caaaaacatg
    16441 atcaggatca agaaaaaata gtcatgtgca aagggcttga tggaatcccc agtgacgtca
    16501 aacccagagt tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac ggcatcacgc
    16561 caacgatcaa aatagcctct gtaataaaaa taaatagaaa agaatgtccc aaaaagtaat
    16621 tgaaaccaga ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc agacaaacgc
    16681 ggttgattca tatccgtcat gatcagctgt cgctgtttca gtgtgatccg tcgtttagaa
    16741 aaaatgacaa aagcagtaga aatcatcatg atccccaaac caccaacttg aaataatatt
    16801 tccagcagta taataccgtt atcattgaac acagaattga tatcaaaagt cgatagtcca
    16861 gtcacactca ctgtactgat tgccatgaac agcatgtcca caaaagaaac atggctatct
    16921 ggttcgcgaa aaatcggcat ataaaataga acgagtgata agaccgtcat caatatataa
    16981 taaaaaacga tgatttggat cgatgagaaa tgattggcag caaacttctg tcctcgccgc
    17041 tgcagccttc gaaagaataa atcaacatgc acagtactat cctcctgaca atagtcgttt
    17101 ctaccagtat acaaggggaa ttaggaatac tcaaagaaat tatcaaacag ataacaatac
    17161 agctttcgat taaaatagaa atggcttata ataaggaaaa tcctagaaaa aagaaggagg
    17221 aggaataaca aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa tcaaaaaata
    17281 aattatgata cagttctgaa aaaaatgatc atgcaatggg agaaagaaga agtaaagcct
    17341 aagatattaa tacatacatg ctgtgctcct tgcagtacgc acacattgga attcatgtct
    17401 caatatgcag agatcacgtt atttttttca aattcgaata ttcatcctaa aagtgagtat
    17461 ttgcgaagat tacatgaaca aaaacgattt gtagaccaat ttaatgagaa aactgaccat
    17521 catgttgcgc taatcgttga tgaataccgt ccaaatgaat tcaccaaaat ggttttaaca
    17581 aaccatcttg aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt caatatgcga
    17641 ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg aagtgccctg
    17701 accatctcgc caaagaaaaa tgcagctttg atcaatcaga tcggaatgga tatccaaaag
    17761 atttacggga cacaatactt gccaagtgat ttcaagaaaa acaaaggcta tgaacgctct
    17821 ttgcaaatgt gcaaagagta tgatatttat cgccaatgtt attgtggttg cgtgttcgct
    17881 gccaagcagc aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc ttacttaaaa
    17941 acatcaaccg tagtttttga ggaaaattaa agaaatcttt cggaaaaacg taagaaaaaa
    18001 ataaagaatt tatggtagaa ataaggtaga ctgtataatt tgtccgtagt aaagtaagga
    18061 aacaagaata aatttcaatg aaggaagaga taagatgcga aaagtagcac ctattttgaa
    18121 gccaatcgct gtattaacaa tctcgacgat tgtcgcgact actacaggta ttgtgaatag
    18181 ttccagctac cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa caccaactac
    18241 ttcagtctcg acatctgaac caacaacaac ggaaagtttg aatgaaacca ctacgacaag
    18301 tacatgggac gatacaacaa ctagttcgtc gtatgatgag acatatacaa ctgacagctc
    18361 aagcgaaaca agtaatacgg taccagatgg cactccatcg gattcaacca atccaagtac
    18421 aagtccggat acaagtgcta gctccgaaac gataccaagc agtacgactg tagatgataa
    18481 cacgcaaaat gatgatgcag cacgtacagc acagcaaaac caagattcag gaaataccca
    18541 aacgtataac cagcaagcag gtgattctca atgacatttt tactagacct tatcaacggg
    18601 gtgcataaat tccttggcta tttagacatc agtcctaaat atcttaaccg tggatatacg
    18661 attttaagtg tgattcccac gctttattta ttgagaattg tttatggact ttggcaaaac
    18721 caaaactatc ttcaattttt cttatatgga cttgcatttc tagtgcttgt ttattttact
    18781 gttttaaatg tattttatta ctttttagat aaaaacacga aagccgatgt cactcagctt
    18841 tttgtgaagt atttacctga tgaagcgttc aacatccaga cagaagaatc gatagataat
    18901 ggaaattcga tcgataaagt gaatacacaa gaggtcatgg tttcttatga cgaagattat
    18961 caattgaaat tagccgagaa tatgcggtat ttgattggaa atggtgagat caaaacaaat
    19021 gaactcggtc gcatggatgg atttctggtc gatagaaata cattgtatcc ttattactat
    19081 gtcaagaaaa caggagagaa agagtatgtt ctaaatattg gacgaagcta ctcggacatg
    19141 gaaaaagtag ggaccattcg acttgactca acagaagaca cattggaacc tgtcggattg
    19201 ttcatcgtcg gcggtgattt tgtcaaagac gggttacgct atcatgagcc ttatcgtctg
    19261 aaattgattg caaaaaaggc acagtcgcag gaagaaccag tggtttatag tcgtagccaa
    19321 agaagaagac attcataatg tgatgaaacg ccgtgtatca gtggttgcga taaaaacgtt
    19381 tattcggaat aagggggatg tgacacgtct tttgtcacgt tcctttttta tataacgaga
    19441 aagataagac gaacacatca cttattcttt cctttcattt ataaacattg tagaataaag
    19501 aaagtacaac gaagtattgt gaaaaaacgt aataaataac gaggaaaata caagtaggag
    19561 gtaagctgtg aaagaatttt tgaaaaattg ggggattttg atccttgtat tagcggctat
    19621 tttattagct agagtgtacg tgtttacacc agttactgta aacgggcatt cgatggaccc
    19681 tacattaagt gatggacaac gattgatttc atctaagatc tcaaactatg aacgaatgga
    19741 tattattact acaaaggaac cgggggatga agaacggatg attgttaaac ggatcatcgg
    19801 tatgcctgga gatacagtca agatggaaaa tgaccagttg accattaatg gcaaaaagta
    19861 tgatgaacct tatctggatg agttcaaaaa agaattttca gaagataagt tacaaggaga
    19921 atatgcttat agctcaggat tccaagcaca agcagaaagt tcatccacct ttacgagtga
    19981 ctttgaatat actgtgccaa aaggaaagta tcttgttcta ggcgataacc gcttgatttc
    20041 aaaagacagc cgaatgtttg ggttagtcga caaagatatg atccaaggaa aagttgtttt
    20101 ccgttattgg cctctttcag aaattaaaat catgtgatga aaaaataaaa agaagctgtg
    20161 acatgttttt tgtcacagct tctttttccg aaaattcctt cttatttctt ggagttgctc
    20221 actttttttg caatctcatt ttaagctttt tggctgatgg ataagaaaag cgaagcgttt
    20281 ttgttcataa atagcttttc cccacgatgg aaggtatgac tagtagcaga gacaaagccc
    20341 gttttttcca atcgattatt caattcttct tggctaaagc cattatggac tttcggatga
    20401 ctaatttgtt cattcttgtc aaagtctacg atgatcaatt gaccgccagg agctaaaatt
    20461 tcataaagtt ttgtaagaat attctccgta tcaggaatgt gtaaaagtac aagtgataga
    20521 aggatgagat tagcttggac agcaggaaat tcgacagaag catcagcgtg gatcgttcgg
    20581 acatttttca gatctgctgc ttggatcttt tcttctgcca ttttcagcat agtttctgaa
    20641 gcatcggcaa tgatcagttc tttgaatctt tccgcaagcg gtaaactaac aagcccagtt
    20701 ccgcctccgt aatccagcag gactttttgg tggctgtcgc gcggtagata agtagttagt
    20761 tcttcgcgaa taatcttggc taatgcttga cggtcagggg aatcataatg gttagcgatg
    20821 gaatcaaaaa tattttttgt catcaaatag ctccttacgt aaaactttga aattttaact
    20881 tattatagca cgtgaaaaga aagaaagcat gctatactca attcaaaaag ggagaatcta
    20941 gaaagaatat gccgatatcg tttgtaaaag atagagagga aaaaggaaag tgtgtccgag
    21001 aaatattact agacttgcct gagtggtttg ggcttcctga atcaacggaa aaatatatcg
    21061 aggaatcaag taaactacct ttatggtgtg aaaaaagaaa agaagaatat ttaggcttta
    21121 tcactctttc acagacaagt gaagatactg ccgaaatcta ttctattgta tgggaataaa
    21181 aaaagactat catcaccaag gattaggaac aaaacttttt aaagaagcag aaggttacgc
    21241 agcaaaacat tacaaatatt tgcaagtgaa gactgtagac gaggggcact actctatata
    21301 tgatcaaact atttgtttct acgaatcatt aggattttca aggttggaag tttttccaaa
    21361 tttatgggat gaatggaacc catgtttagt tcttgtcaaa aaattggaac aaaagtgagc
    21421 aatagattta atactaatct gtgcttgatg aaaattctca ctgtaaaaaa tgctgaaaat
    21481 caagtataat accataggaa caaactggaa ggtgaaaaag agcatggcac aactattttt
    21541 taagtatggc gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg cacataatta
    21601 tgaagaacaa aataagccag ttgtcttaat gacaagcggt attgatacaa gagatggttt
    21661 aggcgttgtt tcaagccgaa tcggactgaa acgtaatgcg atgccgatat ttgaagaaac
    21721 agacgtattt gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc tgatcgatga
    21781 agcgcaattc ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg acgaattgga
    21841 tattccggtt atggcatttg gtttgaaaaa tgattttcga aacgagttat ttgaaggatc
    21901 aaaatattta ttgctttatg cagacaaaat cgaagaaatg aaaacaatct gttggttctg
    21961 ccacaaaaaa gcaatcatga atttacatta tattgatgga aaccctgttt atgaaggcga
    22021 tcaagtgcaa attggcggaa atgaagctta ttaccccgtt tgtcgaaaac attactttca
    22081 cccatcaatg attacagaag gagaataagc atgtacgatc aattacaagc aattgaagac
    22141 cgttatgaag agcttggaga acttttaagt gacccagaag tgatttctga tacaaagcgt
    22201 tttatgcagc tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt ttatcgtcgt
    22261 tataaagaag tagtacaagg aatcaccgat acagaagaac tgcttggtga aaaactggat
    22321 gatgaaatgg ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga aaaagaagtc
    22381 ttagaagaaa aaatcaagat cttgctttta ccaaaagatc caaatgacga taaaaatatc
    22441 atcatggaaa tccgtggagc agcaggcgga gacgaagcag cattattcgc tggcgatctt
    22501 ttcaatatgt accaaaagta tgctgaagca caaggctgga aaacagaagt gatggaagct
    22561 aacgttaccg gaatcggtgg atataaagaa gtgatcatga tgatcaccgg agacaacgta
    22621 tattcgaaat taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc ttcaactgaa
    22681 tcgcaagggc gtatccatac ttccaccgca acagttgtcg tgatgccaga agctgaagaa
    22741 gtagagatcg atattgaaga caaagatatc cgtacagata tctatcatgc ttcaggtgct
    22801 ggcggacagc acgtcaataa aacagcatct gctgtacgtt tgactcactt accaacaggg
    22861 atcgttgttg ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa agcaatgaaa
    22921 atcttgcgtg ctcgtgtgta cgataagatc cagcaagaag cacaaagcga gtatgatgcc
    22981 aatcgaaaat cagcagtcgg aactggggat cgttcagaac ggatccggac gtataatttc
    23041 ccgcaaaatc gtgtgactga tcatcgtatc ggattaacta tccaaaaatt agatcagatt
    23101 ttagcaggga aattagatga gatcgttgat gcattgatca tgtatgatca aacatcaaaa
    23161 ctggaagcta tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac gggcttcttc
    23221 ttttttagag gatcatggga aagaagcata tagtatccaa tttttatttt tggaacgaaa
    23281 acaatggaaa aaacttgatt ggctccttca tatgaacgaa gagatttcag aagaagaaca
    23341 gcggttgatc gaaaccgatc tgcgacttct gcttgcagat catccacctc agtatttgct
    23401 aggatatgca gatttttatg atcatcggtt aaaggtaact gaagcaacac tgatccctcg
    23461 accagaaaca gaggagttag tcgaatggtg tttagacgag acgcctggtg taccgttaga
    23521 agtgatagat attgggacag ggactggagc aattgctatt agtttgaaag cagcaagaaa
    23581 aaattggcat atatctgctg tggatctttc agaagaagca ttagaagtag cgaaagaaaa
    23641 tgctcagaaa gaaggaacga aaatcagttt ttatcatgga gatacattag gaccagtaat
    23701 ggatcagcaa tttgatgtga ttatttctaa tccgccgtac atcagtcgga atgaatggga
    23761 actgatggat gaaagtgtcc ggtcattcga acctaaaatg gccctttttg ctgagaacga
    23821 tggattagct atttatgaaa aaatagcagt tgaagcacca agcgtactaa aatcaaatgg
    23881 aaagattttt ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa tatttcagca
    23941 agcatttcca gataaaaaag tggcgataaa aaaggatctt tttggcaatg aacgaatgat
    24001 ccaagtttat ggttgaagtt gtggatgaaa aattttttat ttgttttttt attataaaaa
    24061 aacacttatt tgacaaggat gtaaacgact ttttggttta tcaacaagtt tatccactat
    24121 tgacaaatag attatccaca aacagtggaa aacttgagga taaactcaga caaaataaga
    24181 atgtatgaaa aaggggttgt ggataaactg tggaaacgaa acgatatgat gaaacagaat
    24241 tgcaagaggc agccgaagca ctgaaagaag gagaacttgt tgcttttcct accgaaacag
    24301 tatacggatt gggcgccaac gctcttttac caaacgctgt caagaaagtg ttttctgtaa
    24361 aagggagacc gcaagataat ccattaatcg tccatgtcgc ctcttttgaa caagtaaaag
    24421 agtatgtgga caactttcat ccggcagcag agaagattgt gaagaacttc tggcctggac
    24481 cattaacttt gatatttaag atcaaaaaag acactttgcc ctcagttgtg acgggaggat
    24541 tatccacagc tgcatttcgt ataccggata acaaaaaaac actagaagta atcgaacttt
    24601 ccggtgttcc gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt ccaacgactg
    24661 cagatcatgt atatcatgat ctacaaggaa aaattaccgg gattattgat gatggagcta
    24721 cgcgaattgg tgtagaatcc actgttcttg atttaagtga tccaacagcc atgcctatga
    24781 ttttacgacc aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa tctcctgttg
    24841 ctattgatca gcatcttgta aaagaaaatg aaacaccaaa agcccctggg atgaaataca
    24901 aacactactc acccgataca cgtgttctga tggtcagaga tggcgattgg tcaactgcag
    24961 ttcaatgggc aaaaaataag aagatccgtg caggtgtcat agcaagcccc gaaattgcgg
    25021 atcaagtgcg gacagatact gcagctgttt atatgtataa cgacaactca gtagaagcgg
    25081 cggctaaagg attgtttgca ggtctaagag gattagatga accaacactt ggattagatt
    25141 tgatttttgt acaagtgtat ccagaaactg gattggggaa tgcttacatg aatcgcttga
    25201 aaaaagcagc aggacaaaat tattttgaaa aatgatcaat tggttattgt cgcattcttt
    25261 tagtatgata caatgctatc aagtaaatct tctatcagtg aacgaaaaac taagcgtaac
    25321 taatcgtaat agaagagata cgaatatttg gcaatttgtt tttgatggga aaaataaaat
    25381 tgtaggaaaa taaaaaggag ctgttgtttg tggtagatta caaaacgttt gaccctgatc
    25441 tttgggctgc gatcgccaaa gaagaagaaa gacaagaaca taatttggag ctgattgcct
    25501 ctgaaaactt tgtctccgaa gcagtgatgg ctgcacaagg aagtatcttg acaaataaat
    25561 acgcagaagg ttatccaggg catcgctatt atggtgggtg tgaatttgtc gatatcgttg
    25621 aaaatttagc gattgaccgt gcaaaagaat tattcggagc gaaatttgcg aacgtccagc
    25681 ctcactccgg ctctcaagcc aacacggctg cttatctcgc tttggtagaa ccaggggaca
    25741 cgatcttagg aatggatctt tccgcaggcg gacatctaac acatggatca ccagtcaact
    25801 tcagtggcaa aacctatcac tttgttgctt acggtgttga tccgacaaca gaagttattg
    25861 attataatgt ggtacgtatt ttagctagaa aacatcaacc aaaattgatc gtagcaggtg
    25921 ccagtgctta cggacgaacg atcgactttg cgaaattcag agaaatcgca gatgaagtag
    25981 gcgcaaaact gatggtagat atggcacata tcgcaggatt agtagcagca ggacttcatc
    26041 ctaatccagt tccttatgca gatatcacaa cgactaccac tcataaaaca ttgcgtggcc
    26101 ctcgaggcgg catgattttg acaaacgatg aagcattagc aaaaaaaata aatagtgcgg
    26161 tatttcctgg tattcaaggc ggccctctag agcatgtgat tgcgggtaaa gcagtagcat
    26221 tcaaagaagc attagatcct gcatttaaag aatatagtga acaaatcatc gccaatgcaa
    26281 aagcaatggt caaagttttc aatcaagcaa ttggaaccag agtcatttca ggggcgacag
    26341 ataatcatct gatgttgatc gatgttcgcg agttaggtat aaatggaaaa gaagcagaga
    26401 gcattttgga cagtgtgaat atcactgtaa ataaaaactc gattcctttt gaaacactaa
    26461 gtccattcaa aacaagcggt attcgaatcg gcacgccagc aatcactact cgcgggttca
    26521 aagaagaaga tgctgtaaaa gtggctgaac tagttgtcaa agcattacaa gcaaaagatg
    26581 ataacgctca actagacgaa gtcaaaacag gtgtacgcga attaactgaa aaatttccgt
    26641 tgcataaaaa ataaaaattt aaattccatt agtaaacaga aaagttcaat gttgggcttt
    26701 tctgtttatt ttttgggaaa acggattctt tgacaatatc ggtgatgctc tctagtatat
    26761 gaggtgtaat tttagtcttc acactaaatc tcagggagga aaagtcatga ctgatcgtat
    26821 ctcgttagaa aaggaagcag tagcattcag tgatgcaaat gcgcctcatc cgcgtatcta
    26881 tgaactagca ccggaagaag gaagagtttt actagaagaa gtacaagaat cacctgtgaa
    26941 caaatattcc gtcgatattg aagacttgat ggtggatact ggcgaatggg gaaaactcaa
    27001 tattcgtctc gtacgaccag aagggaatat ggataaatta ccagttattt tttatattca
    27061 tggagcaggc tgggtttttg gcagtgctca aacccatgat aaattgatca gagaacttgc
    27121 ggttcgtacg aattccgtcg ttgtctttcc agaatatagt cgatcaccag aagcaaaata
    27181 tccaacagca attgaacaaa attatgctgt gttgcaacag ctgaataaat tagccaagtc
    27241 aaaaaatctg gatctcgaga atctaatcgt tgcaggagat tcagtaggtg gtaacatggc
    27301 tacagtcatg acgatcttaa caaaagaacg aaagggcctc ccaatatcca agcaattgct
    27361 gtattatcca gtcacaaacg ctgattttga tacagattcc tatcatgaat tcggagaaaa
    27421 ctattttttg acaagagaag gaatgatgtg gttttgggac caatatacga cgaatgaaga
    27481 agagcgtgct gaaatcacag cttcgccttt gagagcaaat ataaatgaac taaatgatct
    27541 tccttcagca atgattttaa cgggagaagc ggatgtatta agagatgaag gtgaggctta
    27601 tgcgcgcaaa cttcgtgatg caggggttcc tgttacacaa gtacgcttcc aaggaatcat
    27661 ccatgatttt gtcatggtaa atgctatgga tcagacaaag gcaacgcgtg cagcaatggc
    27721 attatctact tcttggatca agaaaaaaga ataagctttg cttgtccgca ggaaaacttc
    27781 ttttcaaaaa cggattttct taggaaaaat ggcgagtttg actagtcaat cacttgattt
    27841 tgttttacaa ttaaaaagaa acgacagaac aaaggagagc ttaaaaaatg ggcaaatttc
    27901 aagtgatcga tcatccattg attcaacaca aactaactat tattcgggac aaaaactgcg
    27961 gaacaaaggt ttttcgtgaa gtagtcgatg aaatcgctat gttgatggca tatgaagtat
    28021 cacgggatat gccattggaa gacatcgtga tcgaaacacc gatcactgaa acaacacaaa
    28081 aaacgttgtc tggtaaaaaa gtagcgatta ttccaatctt gcgcgcaggt attggaatgg
    28141 ttgacggtat cttggaattg attccagctg ctaaagtggg acatatcggt ctttatcgag
    28201 atgaagaaac attggaacca catgaatatt tcgtcaaact tccagaagac attgatgcac
    28261 gtcagttgtt tgtagtagat ccaatgttgg caacaggcgg atcagcaatc atggcaatcg
    28321 atgcattgaa agaacgtggc gcgtcgaaca ttaaatttgt ttgtttggta gcagcacctg
    28381 agggagtaaa agctttgcaa gaagcgcatc ctgatattga catctataca gcttctctag
    28441 atgaaaaatt gaatgaacat ggttatatcg ttcctggact aggggatgcg ggtgaccgtt
    28501 tgtttggtac aaaataagta aatacgaaaa agggtgtgac acaacttttg tcacatcctt
    28561 ttttggtcat ttttaacgtt tgatgcagaa cagctttttc acagctttcg tttgcatata
    28621 aaaaaaccat cccaaaaatg cagagatggt caaattcaaa tcttttttag ttctttgtag
    28681 ttaacgatta ctacaccctt actttgcgtc atcaagttcg cattttctgc agtttcatca
    28741 atctcaacaa cagcggaatt tttcaattgc tttgaaatcg ttccagtttg cgacttgccg
    28801 tgaattgtga acattacgtg agtattgatc ttgaattttt caccaatttc cgatggtacg
    28861 atagttgtat caaattttcc aaaagttgcc attttcataa aacctgcctt tcacatcaat
    28921 tataccacgt tttatgaaaa atatcatgaa attaaactaa ttttccatcg gactctttac
    28981 aatcagccaa atagagtata taatttcttt tggataaggc cagcttagct cagttggtag
    29041 agcaacgcac tcgtaacgcg taggtcgtag gttcgatccc tatagctggc attaaaagag
    29101 aagacagata tatcaaggat ttttaatcca ttgatacatc tgtctttttg tttaaaggaa
    29161 ataaagtatg ctttcttagc aatagagcat gtattatttc tataaaaatt taggagaata
    29221 aaacgactag caatatagct gtgaaatcaa atatcacatg aactgcccaa cttgtaatga
    29281 tcgagttgct tttaatggca gcttggttga ataacaatct tgccacccct tgtatcaaaa
    29341 ggatgtgtgc tacagtgtgt aaaatatttc cattatcata agtactgtaa tggataaatc
    29401 caaagatgat tgctgacaag aaactagcga caggaatgct gaacttacta gaaaaaatag
    29461 cgagaaaata cactgagaac aattcttctc caagtatcat gataggcaaa ataataaata
    29521 ataacgggct gaaagcctca tttattggat tgcctgttaa accccatttc aaaatttgtt
    29581 gcaaaaatac cgagactgcg gcactgatta ctacattgat aaaaaaatat ttggctatat
    29641 ttttgacagg agcgacaggt ttcgagaaca tttttttcgt atctttttta ccaaaaacaa
    29701 gtgtaaatgc tataaatcca gtgatagtta aagaaaaaat gctaataatg gatggcgcaa
    29761 atagcaaccc taacaaaccg agtacaacga caatagtgcc caatatccct tttttaagtt
    29821 ctattttttt tcctaaaatg ttattcatga taataactcc tactattcag tttttgaaga
    29881 tagagtcagt atacatgaag agttaaaata ggcaatgttt ttttatggat atttgtattg
    29941 ctaaaaattt atcttatata ttcattttga ttatactact tttatttgta aaaattttct
    30001 agcaaaacca agtgcttgca actgattaag cgtacaattt tttagatgtt ccgagcgtac
    30061 ttcaatcatt tcaaaggagt tgctacttaa atcgatattt tttaaatcag tatcaaaaaa
    30121 gctagcattt tctaaatcat tagtagaaaa cattatttta gtgagggaac agtctacaaa
    30181 atctgcgctt gataagacag agtgctcaaa ggtactttct tttatgtttg aaccagataa
    30241 atttacatag tccattttac aattctcgaa taacgtgttc ccaatataac tttccatcaa
    30301 tttagtacct gttaatttac aattgacaaa tttcacgcga tgcaacatgc tttttgtcaa
    30361 tatacagcct gaaaaatcac agttttcgaa tatacagtcg gtaatctcca ttctttgaaa
    30421 atcattggaa gcaaaaaatg catacttgaa aatagatttg taaataattg gatggcagat
    30481 tatttggttt tcgatgacct ctttttgtga aaggatatta tacagttcaa aatcttcgtt
    30541 ttcatagata tgaaaaaagg atcctttatt caagcttgct tcattaactt taggaaaaat
    30601 catatttaat tacctccatt ttcataaagt catattaaca aaagctaaac caacttacat
    30661 tagcagaaaa tgagataggt tttcgccttt ttcgataacg atagtgtcgc tatctcttga
    30721 aacgttctta gaattgccct gagagacaca aattaggcgt aattctaata aaaatcttag
    30781 aataaaaatt tttactgatt taatttagaa tgtttttaaa ataaaaatcg ttgccataac
    30841 agtgtattga aaggttttaa caatccagta atgatttaaa aaaatctaga tgtagcgtat
    30901 actttgttta tagaatgtag tgggaagaaa tatatttttg ggaggaaatc gtgatgtcta
    30961 aaagtgaaga aatagaaaac atggtactaa aattatatga tctcgtttta aatccagaga
    31021 tcaaagaaaa ggaacgtata cttttgatag acgcaaagac aggcttagaa aaaggtcagt
    31081 attatcctaa agtcataaat aatttggaac gtagtttacg tccgttagca atcaggggag
    31141 aattgtctaa accagtatca ccgttttata tggagatatc aacaatagga aaatttgaaa
    31201 aagaattagg tcgaggaatg gcttcggcac cgattacttt tgggcattta taaaaacgag
    31261 ttattcatct caatgaatta aaaagtatag tcaatctcat aatgcaggct aggttcattt
    31321 atttgaacag agtctgt
//
LOCUS       DAFCNS010000033        31090 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFCNS010000033 DAFCNS010000000
VERSION     DAFCNS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.033"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(19..729)
                     /locus_tag="LNE23_001394"
     CDS             complement(19..729)
                     /locus_tag="LNE23_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBK6931253.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="LNE23_001395"
     CDS             complement(726..2198)
                     /locus_tag="LNE23_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK6931254.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="LNE23_001396"
     CDS             2336..2809
                     /locus_tag="LNE23_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6931255.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="LNE23_001397"
     CDS             complement(2962..3513)
                     /locus_tag="LNE23_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6931256.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="LNE23_001398"
     CDS             3618..4148
                     /locus_tag="LNE23_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBK6931257.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="LNE23_001399"
     CDS             complement(4631..5074)
                     /locus_tag="LNE23_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6931258.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="LNE23_001400"
     CDS             complement(5090..6250)
                     /locus_tag="LNE23_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6931259.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="LNE23_001401"
     CDS             6757..8457
                     /locus_tag="LNE23_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931260.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="LNE23_001402"
     CDS             complement(8559..9392)
                     /locus_tag="LNE23_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBK6931261.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="LNE23_001403"
     CDS             complement(9696..11861)
                     /locus_tag="LNE23_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6931262.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="LNE23_001404"
     CDS             12121..14415
                     /locus_tag="LNE23_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK6931263.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="LNE23_001405"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="LNE23_001405"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK6931264.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="LNE23_001406"
     CDS             15066..16073
                     /locus_tag="LNE23_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK6931265.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="LNE23_001407"
     CDS             16095..17114
                     /locus_tag="LNE23_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6931266.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="LNE23_001408"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="LNE23_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBK6931267.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /locus_tag="LNE23_001409"
     CDS             complement(18871..20292)
                     /locus_tag="LNE23_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6931268.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="LNE23_001410"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="LNE23_001410"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK6931269.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /locus_tag="LNE23_001411"
     CDS             complement(22703..23959)
                     /locus_tag="LNE23_001411"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBK6931270.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24071..24276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24539..25840
                     /locus_tag="LNE23_001412"
     CDS             24539..25840
                     /locus_tag="LNE23_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK6931271.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="LNE23_001413"
     CDS             complement(25909..26508)
                     /locus_tag="LNE23_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6931272.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26762..27643
                     /locus_tag="LNE23_001414"
     CDS             26762..27643
                     /locus_tag="LNE23_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931273.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27656..29266
                     /locus_tag="LNE23_001415"
     CDS             27656..29266
                     /locus_tag="LNE23_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBK6931274.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29343..30002)
                     /locus_tag="LNE23_001416"
     CDS             complement(29343..30002)
                     /locus_tag="LNE23_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK6931275.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30285..30950)
                     /locus_tag="LNE23_001417"
     CDS             complement(30285..30950)
                     /locus_tag="LNE23_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBK6931276.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         9296 a         6341 c         5644 g         9809 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 taaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatgtata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    26641 tataaaaaga atattatgta ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc
    26701 tttttattaa actatttaat cttgtttagt atatattttt agaaaaagga gtggggtttt
    26761 tatggcagaa atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt
    26821 aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg
    26881 ttctggaaaa tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac
    26941 ggcaacgatt tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc
    27001 ttatgttcca ggcgatatct ctttgtgggg aaatttgact ggaggagcga tcatcgatct
    27061 cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt
    27121 ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg
    27181 cttgattgct gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg
    27241 tttagatcct ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg
    27301 caaatccatc ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt
    27361 cgtgatcata cgcgaaggaa cactcgatga agaaggaaca ctcgatgaat tgcgtcattt
    27421 gactcgttcg accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa
    27481 cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc
    27541 tttcgatgtg attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc
    27601 accgactctt gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac
    27661 tgataaattt tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa
    27721 gatcctgata tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga
    27781 agaattgact tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat
    27841 gattgcaatg gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat
    27901 gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca
    27961 cgtggtgagc caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt
    28021 tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt
    28081 gcttttagca atatcgatca gctgtacgat gatttttttc caagcggatt cgatcactgc
    28141 agaaggtgct tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt
    28201 tgcactgttt gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc
    28261 gtttttaggt ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc
    28321 gatgtggaat cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat
    28381 tcctttgctt tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga
    28441 ggaacatcga gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa
    28501 acgttctctt ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag
    28561 ttggcttatc acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca
    28621 acagttttta gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat
    28681 tgaaaaaaac tttacaagca cgatcatggt agtcttgatt tgtctagctg ctattttacc
    28741 aattgctagc atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt
    28801 cgcaacaaaa atttctagga accaacttta ttggaccaat atgttactag ctattctatt
    28861 tgctatattg gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat
    28921 gaaggatgcg aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc
    28981 tgccgtttta gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatcgg
    29041 taaacttgct tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt
    29101 ggatttacca gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa
    29161 cgattctttt gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt
    29221 ccttggttct attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta
    29281 caatttcatg ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt
    29341 atttacaagt cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt
    29401 acataaccat tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg
    29461 ataaatgcag caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct
    29521 ttagctaagt caagcagata gacagcgact cgttcttcta ctttttccat catcaaaaaa
    29581 gttgtctgct gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta
    29641 agggcgggat aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc
    29701 gttttttcta gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct
    29761 ccctcatatc cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat
    29821 tgatacactt tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca
    29881 agaatctgtt ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga
    29941 tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc
    30001 ataatctctc ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc
    30061 aactgataat tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag
    30121 agtggatttc tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat
    30181 tcctatcaat tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa
    30241 gccgagagta aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc
    30301 gcacctagct tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca
    30361 acgtgttcga tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc
    30421 atcgcttttt tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag
    30481 tggtcgtcaa gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt
    30541 ttgctccact ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt
    30601 tcttcgcttt tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa
    30661 actgctagac aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga
    30721 ctgcgtaaat tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata
    30781 tcaggcaaaa gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta
    30841 tattctttct tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat
    30901 gcgtagattg gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct
    30961 ctttccgtta tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga
    31021 aaaagctgtt ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac
    31081 cgcagtttta
//
LOCUS       DAFCNS010000034        30862 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFCNS010000034 DAFCNS010000000
VERSION     DAFCNS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.034"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(118..663)
                     /locus_tag="LNE23_001418"
     CDS             complement(118..663)
                     /locus_tag="LNE23_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6931277.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(675..1367)
                     /locus_tag="LNE23_001419"
     CDS             complement(675..1367)
                     /locus_tag="LNE23_001419"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBK6931278.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1386..1670)
                     /locus_tag="LNE23_001420"
     CDS             complement(1386..1670)
                     /locus_tag="LNE23_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931279.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1683..2246)
                     /locus_tag="LNE23_001421"
     CDS             complement(1683..2246)
                     /locus_tag="LNE23_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK6931280.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2474..3136
                     /locus_tag="LNE23_001422"
     CDS             2474..3136
                     /locus_tag="LNE23_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBK6931281.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3260..4456
                     /locus_tag="LNE23_001423"
     CDS             3260..4456
                     /locus_tag="LNE23_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBK6931282.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4557..5933)
                     /locus_tag="LNE23_001424"
     CDS             complement(4557..5933)
                     /locus_tag="LNE23_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6931283.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5970..6836)
                     /locus_tag="LNE23_001425"
     CDS             complement(5970..6836)
                     /locus_tag="LNE23_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBK6931284.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6925..7950)
                     /locus_tag="LNE23_001426"
     CDS             complement(6925..7950)
                     /locus_tag="LNE23_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6931285.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7968..8642)
                     /locus_tag="LNE23_001427"
     CDS             complement(7968..8642)
                     /locus_tag="LNE23_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6931286.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIKGYIETKVG
                     QGYIVP"
     gene            complement(8826..9719)
                     /locus_tag="LNE23_001428"
     CDS             complement(8826..9719)
                     /locus_tag="LNE23_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6931287.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9793..11490)
                     /locus_tag="LNE23_001429"
     CDS             complement(9793..11490)
                     /locus_tag="LNE23_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6931288.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11483..12796)
                     /locus_tag="LNE23_001430"
     CDS             complement(11483..12796)
                     /locus_tag="LNE23_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK6931289.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12796..16833)
                     /locus_tag="LNE23_001431"
     CDS             complement(12796..16833)
                     /locus_tag="LNE23_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK6931290.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FHHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16846..17304)
                     /locus_tag="LNE23_001432"
     CDS             complement(16846..17304)
                     /locus_tag="LNE23_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6931291.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17602..18120)
                     /locus_tag="LNE23_001433"
     CDS             complement(17602..18120)
                     /locus_tag="LNE23_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBK6931292.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18122..18739)
                     /locus_tag="LNE23_001434"
     CDS             complement(18122..18739)
                     /locus_tag="LNE23_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBK6931293.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18736..19590)
                     /locus_tag="LNE23_001435"
     CDS             complement(18736..19590)
                     /locus_tag="LNE23_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6931294.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19587..20498)
                     /locus_tag="LNE23_001436"
     CDS             complement(19587..20498)
                     /locus_tag="LNE23_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6931295.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20539..21873)
                     /locus_tag="LNE23_001437"
     CDS             complement(20539..21873)
                     /locus_tag="LNE23_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6931296.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22081..22911
                     /locus_tag="LNE23_001438"
     CDS             22081..22911
                     /locus_tag="LNE23_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBK6931297.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23155..23437)
                     /locus_tag="LNE23_001439"
                     /pseudo
     CDS             complement(23155..23437)
                     /locus_tag="LNE23_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23595..23789)
                     /locus_tag="LNE23_001440"
     CDS             complement(23595..23789)
                     /locus_tag="LNE23_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931298.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24294..24479)
                     /locus_tag="LNE23_001441"
     CDS             complement(24294..24479)
                     /locus_tag="LNE23_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931299.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25329..25670)
                     /locus_tag="LNE23_001442"
     CDS             complement(25329..25670)
                     /locus_tag="LNE23_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK6931300.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25670..25900)
                     /locus_tag="LNE23_001443"
     CDS             complement(25670..25900)
                     /locus_tag="LNE23_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6931301.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26187..26525
                     /locus_tag="LNE23_001444"
     CDS             26187..26525
                     /locus_tag="LNE23_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931302.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26730..26969)
                     /locus_tag="LNE23_001445"
     CDS             complement(26730..26969)
                     /locus_tag="LNE23_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBK6931303.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(26945..27292)
                     /locus_tag="LNE23_001446"
     CDS             complement(26945..27292)
                     /locus_tag="LNE23_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBK6931304.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(28024..28605)
                     /locus_tag="LNE23_001447"
     CDS             complement(28024..28605)
                     /locus_tag="LNE23_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6931305.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28788..29414)
                     /locus_tag="LNE23_001448"
     CDS             complement(28788..29414)
                     /locus_tag="LNE23_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6931306.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29436..30767)
                     /locus_tag="LNE23_001449"
     CDS             complement(29436..30767)
                     /locus_tag="LNE23_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBK6931307.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
BASE COUNT         9104 a         6596 c         5239 g         9923 t
ORIGIN      
        1 aaaaagaata agttttttta aaataacgta taaaaaagag cataaaacaa agtcgtttgt
       61 tttatgctct tcttttatta ttttgtgctt tttattttat caaatcactt tgttttttta
      121 taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat gtccaacggg
      181 atcgaaactt gccacggaat ctttgaaaat atggagtttg tatcctagat tatacgcatc
      241 gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa tatctgtgat
      301 ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat aatgccgttt
      361 gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt ttaaagagcc
      421 gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc ggttctccgg
      481 atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat ccttttgatc
      541 aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg ctgtcccagt
      601 agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt
      661 catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc tgctgatcgt
      721 ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg
      781 ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg tccaacagcg
      841 gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc tttgatttta
      901 tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc gttcaaattc
      961 gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc ataatataga
     1021 ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc atcaaaataa
     1081 gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat tccgccagct
     1141 gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact tgtggtaata
     1201 gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt acctaatgaa
     1261 cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag gttctctctc
     1321 aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt tttaattcct
     1381 tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat aacaataata
     1441 agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct gatccttgtt
     1501 ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat ctttttttct
     1561 tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct tgcttgtgct
     1621 tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat aagccgatcc
     1681 ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt ctttgcatca
     1741 caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg atacaactcc
     1801 agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc ttgtgcatga
     1861 taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt aagatggctc
     1921 agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt catttcggga
     1981 ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac tttttctaaa
     2041 ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg
     2101 acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg caaacgaact
     2161 tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact gattgttttt
     2221 tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat cataactgct
     2281 tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata gataaacctt
     2341 agattttctc taaatataga aaatccttct ttagtccgat actctttcta gtagtccgta
     2401 tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg ataaatgaca
     2461 tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg tcttgctatt
     2521 tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga taggtgtagt
     2581 tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc
     2641 tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat
     2701 ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa
     2761 tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag tcaaagcagc
     2821 gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag caagtcagtt
     2881 tctttatacg catctgccga atctagttga tttgacaaat aagtataatg agatcaatga
     2941 ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc agatcatcga
     3001 ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg atctagatga
     3061 tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata aatatgaacc
     3121 agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc agatgatgtc
     3181 tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg agaacagatt
     3241 caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg ttacaccagt
     3301 taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag atacgctgac
     3361 cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg ctcgtttaat
     3421 tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa aaatcgatgc
     3481 taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat taagtgaatt
     3541 ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag gagattcctt
     3601 aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg ccggagaagg
     3661 gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag tgacagcaaa
     3721 atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata aagaaaaaga
     3781 cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca aagactactt
     3841 tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac ttcaaacaac
     3901 gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg atgttcagat
     3961 tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg atcttcgctt
     4021 agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa acacaaatca
     4081 ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt ggaaaaacca
     4141 agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc aacgccaagt
     4201 ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa tctcagccat
     4261 cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc aaaccactca
     4321 aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag gaaaagaaaa
     4381 acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac aaaaactgtt
     4441 ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca acaacagttt
     4501 attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat tggagtctat
     4561 ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag tccaataacc
     4621 attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa
     4681 gtcaagatgg agacgattgg caatagcgga acgaatggca ctttgaattc tccagctttt
     4741 ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat caaataggcg
     4801 agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat
     4861 acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt ttgatcgatt
     4921 tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa acgagcaagt
     4981 gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac agcgatcacc
     5041 gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc attgactcct
     5101 agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta aaggacagta
     5161 gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt tttgacttcc
     5221 tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag agctgcacca
     5281 tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg tttaacgaaa
     5341 aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac catggcatta
     5401 ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa tatcactacg
     5461 agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc agttaatgct
     5521 tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga ccatccggaa
     5581 gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt cagccaggca
     5641 gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac tggcactcgc
     5701 gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc taagacaaat
     5761 gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac cacaaatatt
     5821 cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt tagttctttt
     5881 ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc cattgaagtt
     5941 cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc taaaaggata
     6001 ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt tttcgttttt
     6061 ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat ttgtgaaagt
     6121 gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat gtttcctaag
     6181 ccccataata atccacagat catgtttttc cacacaaact gatcgacttt aactttacgg
     6241 aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat aattgacatc
     6301 gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat cgtagagaag
     6361 atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc tatttttcct
     6421 tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa agcaataata
     6481 ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta
     6541 ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc catgtacttc
     6601 attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa aaatccgatc
     6661 aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa agaaaacaga
     6721 aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat tttaccactt
     6781 actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat ttccataacc
     6841 aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac aacacaagca
     6901 ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta agattgggaa
     6961 aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat ggatacctaa
     7021 tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg ttttgctaag
     7081 tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt ccgctgggat
     7141 accgatccct gaatctttaa ctgaaaggct gactccttgc gcttcctttt ctatgctcac
     7201 aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt ggttgaagat
     7261 aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg
     7321 aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa tgattccttt
     7381 tagtccatat tcttggatga ggtagtcccg cgagaaactg tcgagtcttg cataatacaa
     7441 aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg tatacttttg
     7501 atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa gtggcacttt
     7561 gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac tgattgctga
     7621 ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta ccagtttttc
     7681 ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc
     7741 taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa
     7801 gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga tgtttggcgc
     7861 cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca
     7921 ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca gggaacgata
     7981 tatccctgtc ctacttttgt ttcgatatat cctttgatac ctgcctgttc gatttttcgg
     8041 cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac
     8101 agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat caaaataaat
     8161 aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc catacttccg
     8221 ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa ttctgtgtag
     8281 ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat ttggaaatgt
     8341 tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat attcgtattc
     8401 ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg acaccaataa
     8461 tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc ttctctttca
     8521 aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg ccatttctca
     8581 agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat gatttttgcc
     8641 ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc cttgtgaaac
     8701 aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg
     8761 atttaatcgc gtttgtctct tccaagcagc ttaaatttat tatgttattc ttaataattg
     8821 gtctattatc tgttattttt ctttcgttgt ttttgtcttt taaattttcg ataacaccaa
     8881 taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa tcgataaaga
     8941 tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg aactcgtctt
     9001 cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact
     9061 agatcctgct tttctctaat tgctaatgta tttgtttttg ttggaaggac aacaattttc
     9121 tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg gtcatcttgc
     9181 tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg ggcggtaatc
     9241 acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac tgtcgctcct
     9301 ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt gattttcgga
     9361 atatagatgg ctccgatcat atgttgttcg taatattctt ttcctggatt ttggacattt
     9421 ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac tgatgtatga
     9481 gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt ttcttcttta
     9541 tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata attgctgaga
     9601 gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat cactgccata
     9661 aataattgta ataaaagact gatttgggtt tgaggtctat tacctatttt ctttttcatt
     9721 ttcttcacct tcttatttaa aaaaactgca gcaaccttta taaaaccgat tgctgcagct
     9781 tatttttgtt gcttatactt cagacttcat tttagattta cggtaccagc tgtacgctcc
     9841 aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac cagtagcagg
     9901 aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta ctcctacgat
     9961 ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg ctgcctcacc
    10021 tgttggaagt acataacctt ctggcgccat ggtctctttc atttgatagg ttccataatc
    10081 taacccttga acctcaaagt atccatttcc atctgcgaca aattctgttg cttgtccgct
    10141 tgttgcccac tcaattctat ctgctcctga ttcataaaca gctaaaggcg actccccttt
    10201 ataaaatatt gcgtattcag aacggtcttc attccacaat tcgaatcttg catctttaag
    10261 caattcactg cttgatgcat catatttttc aaactttctt ccacctgttg tgactttctc
    10321 tggcggctct actccttctt cataatcaaa ttcatcattt cgtccgattt caataaccgc
    10381 tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa accttcaaat tatacgtgat
    10441 cgttaaatct cctccacgtg catctgctaa tctacctaaa gtatctactt tggctactcc
    10501 attttcttct gtcaaaatcg tcacgacaaa cccgttgctt actacttcta ctatataatc
    10561 ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct atttcctgat cataataagt
    10621 tagaccaact gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc ttccgttttc
    10681 aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc
    10741 tctttctaga tcaatataag aaatcgtttc ttgttctcca gcttcattaa taatcgttac
    10801 ttgtctaagg tcgctttcct ctacattcat ctgtttatct gcttctctga tttcgttttt
    10861 cggatacaaa tgcacatctg tattgtatgt gtctttccat gtagaaccat cccacacgcc
    10921 atccaccata tcaggcatca tcactggcat cataacaacc attggagacg cgattctgga
    10981 aatatgagct ggcgaataag tttctaagaa aagatagact ttatctctag aagaaggtgc
    11041 ttcttccgtc acttggacgg tagagaatac cgcttctcca gcttcattcg ttgtttgttt
    11101 gtcaatactt gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt tttgaatcca
    11161 ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag gattatctcg
    11221 aatcaaatcg taatatactt ccgttacgtc aaacaaatca aaatcgactc ctggaatatt
    11281 ttcttcacca aaatccgtag aaaccaatcc gctattcggt cgttcttccg gaacagatga
    11341 gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga cttcttcggc
    11401 tttgactagt tttccgttcc cgaaaatccc tattcccgca gtaacgagta acagtacact
    11461 cattcccgtg acaatcattt ttttattcat ttttttctcc ccgttctttt tttattaata
    11521 aaacagcacc taacaataaa agaattccta gtaaagaaat caacgttctt gcttcattcg
    11581 tttgcgggaa tacggaccat tttcctgatt gacctggatt gcttggacca ctcggctctt
    11641 tcggtttgtc cgttgtttct tttacggtct cgttataaac acgtggtgtt gcggattcat
    11701 aggcagagac tggaactttc ccggattcta attcagccat ctcctgtccg ttaactacta
    11761 catattgtgg ttggcctttt gcatccaccc aaaattcagg gatgatgacc tcgattctct
    11821 tacttgcttc atccatctcg taaccttcca caccttttaa ttcttcaaaa taataggtgc
    11881 cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc tttatccgaa atgaatttgc
    11941 ttacattttt gtcattcaaa ggatcattgt ctgctggttt gatccattga tttttcaagt
    12001 cattagcagg agccatatct aaataatatt tccccccttc tatcatctga tatagagcaa
    12061 aaacagcacc ttctaatggt tttccttttt cagttgttcc tctttgacgt ccatacttga
    12121 agaagtatgg atctcttaga taaccgacat ttttaggata gatatgaatc tcttgcagtt
    12181 cctcggttga ttctgtcggg tgataaattg gcaaaatagc agcaattggg atagctgtct
    12241 gatcgatatc aacatccaat ccaacttctg gatcaatcga ttcttcgaaa atcaaataaa
    12301 ctgcatcccg atcttggttt ttcttaggtg ctttgatacg tgcaatcccg tctcctaatc
    12361 cgccaaccgt atctttccca gttgttaccg aagtccctat ttggacaagc ctttcttctg
    12421 tgattagctg acggatattg tttctgttcg tttcagcgat ttgctggacg aattctttag
    12481 gagtcatgtt ttcagcagct tttttttcat gataataatc ggtcatgtcg taaatggcaa
    12541 atgtcgctcc atttagtggc accgtctgtg aaatgatatc tgctgtttcc cctgcttcct
    12601 cagagatagc caagccatca ttttcatggt attcaaattg atcgttataa tcgatatctc
    12661 gaatcatccg tttatgcaac acgaattcca cgacatcttc ccctgattct tctgccgaca
    12721 tcgcatgtgg aagaaggact gctgcaagcg gtagtaaaac aagtaattga agcatcaaac
    12781 acaacagctt tttcattacc ttgctccctc ctttttagat tgtctgacaa acaaaccata
    12841 ggctgctcct gcaagcgcca tgaagaatgc agcaagcagg atatagaata atcgtccgga
    12901 tcctcctgta gcaggcaacg gattttcagg atcgttcccg acactaagct gtaatctgat
    12961 tcgatttgtc tccaaaagtt catattctaa ctcttgctgt agatcaggcg tctctgcttc
    13021 ttcgtttgga taatacgaaa tctcaacttt tagtgtaccc gcttcttttc ctggaactac
    13081 ccgatcattt tctatgtcag gttcttctcg ataactaatt tctaaaataa agtaacctgg
    13141 aagttttcta tagctgtcag gtgacttcac ttccttgatc cgatagaccc cttccaacaa
    13201 gtctgaagca ttgaataggg aagaatcgtt tggatctggt gccaagcctc cctcgatgat
    13261 ttcatactct cctgcactat tttgtttctc aattgaaaat tctgcgcctt caagacgtcg
    13321 gctattcata ctgtcaattt tcaaaagttc caaatcgatt ggcgctagtt catcttctaa
    13381 ggttacttgt agtacaccat cgatgatttc atagccttcc ggcaagggag cagtttcgtc
    13441 ttcgatgacc gtatcttcaa tgataaactt accttcttca ttcagtgcaa actggaacac
    13501 ttgatcagat agccgatacc catttggtgc gacagattcc gagacctcat attcaccaat
    13561 agacagatgc agaggttggt cgtaatttaa atcgacatac ttttctactc ctacggcacg
    13621 agaatagaag tatccgatcc caggcaaacg tttcgattct tctggttcta acggattacc
    13681 agtgtatgct cgatacagcg ggaaggaaga atcccccact ttcctgatat cgaaacctgc
    13741 accatctaat gtatggcctt catggtctaa ttttctaact cggatctccc gataatcaaa
    13801 cgtattgtaa gcagtgaatg ctacccataa acgatcctct tcatctgttg tatacgtgag
    13861 atattgccaa ttttcttcac ctgtttgctt gagttggata gtccctatag cattatcgta
    13921 aagtcgcaag atccatgtgt cattttcatc aggttctttg aaaccttctg ggttctggct
    13981 ctcaatgatt ttgtattctg catagttggt agtttcttca ctatccttat aaacaaatga
    14041 atctggctct ccgattggat gaataacttt ctcaccatcc acgattacat atttcccgtc
    14101 ttcatctact gaatagaacg tgagtctccc tttctcttcc tctacttcgt tcacaccaga
    14161 aagccctttc gctactggat atccgatttc cagtccgtta ccatctgttt cataaagttc
    14221 aaactctgcg ccattgattg gcatattact gccttcaatt agtttattga tattcatgtg
    14281 gattggcttg aattcatttc gcatttccag tttgacatac aactcatcat actctgtgcc
    14341 ttctatgtta ggctcttgta caacagaaag cttgtgatat tctccttcat aataggaatt
    14401 cggatcttta ccttccggtc cgccgactct cgaaccagat gtatattcat ctacaagctc
    14461 gtagtgacct aacatctcat cttcgttggc tgcatatttt ttgatttgga attgatacgt
    14521 ctcatctact aactgataac gtggtggtcc agcgatctct ctgatccaaa atatgtcgct
    14581 gactgctcca gtttcatctc ctaaaatcgg catcttcgtt tcaaagatcg ctattccgtc
    14641 cgtggtagtt ttacttgttt cgattggttc ggcttcccca ctctctggat tttggtcata
    14701 taatgcaaat tcgactccag ccaacggctc gccattttga cccttcttgc tgacttggaa
    14761 attacggcct ttatgttcgt aacgaatgga agaagatggg aaatacatcc gtccagtagt
    14821 gatatcattc tcataatcct ccaggttctg aatataggcc atattgttca ctgtgcaaaa
    14881 atttccgtct tgagctcccg catcaagttt tacttggtac ttgatcctga ccatttcccc
    14941 ttcacctaaa gaaatcggag aaaatttata agcttggttt tcccgtccga catacgttgt
    15001 cggagtcgcc actacttctg gtggggcttg tccatgacgt tcttcatccc atatttctgg
    15061 ctcttggtac ttcatgccgt tttctcctgg atctagatgg taaaactgaa tatctgccga
    15121 tctgccagct ccaaatggtg agacattaaa accagtttca tcataaagtg tggcttgctg
    15181 gatcgttctc ttgaaggtag aagagatatc ctccaaggct gtaacaatgt cattcttgtt
    15241 gttcgcattg taatatcgcg gtgttacgcc atcttcttct aaatcagcaa tatttttcaa
    15301 tacatttcgt ccaatagcat ctagtcgttg tctcccgcta acgtttccat cgattcctaa
    15361 accaatggag aagatacttg gcgggttctc ttgatacttg tttttcagcc aatgatgata
    15421 agcaactgtg ttgatcgcag atgacttttc ttgcgaagta ggactgccat tcgtattgtt
    15481 attagaagaa ccatcaccaa cccagtgagt cgctttatta tctccttctt ttacctctcc
    15541 ataacgatag tgtacattta cttcatcttc tggacggtta aagtcagatg cataggtaac
    15601 tggatgagta aaccctgcat tagaagtatt cccagaaaac tcctcaaaat ttctgaacaa
    15661 aacgtcatta tcttcagcaa tcttatcttc catcacagtc cagttgctcc aagctccatt
    15721 agtgctcgta cgataacttg actgaatcgg tgcataactg aaggtcggtg tcccatctcc
    15781 cacaacgatt aggatttttt ctggatttct attttctccg ccattgtctt tatacaatgt
    15841 ttcatagcca tttttaagcc ctagtgtaag cggagttcct ccgattggtg tacgatttaa
    15901 tgctgtattc tcacttcccg tcaaacgatt gatatcattt gtcaatggaa agatgtccga
    15961 actgatttgc tcatggttat tgatagctgt actattatgg tgaaaattca ccataccgat
    16021 tcgaatatcc agactcgggt cagagagcag atctttactc atttcgttta ctgcctcaat
    16081 caacgcagca tttttagttt gactattggt cccagcggtt aattcactca tactcgctga
    16141 cttatctaga acaaaaacga tatcgattgg actgatttcc tttttctcgc ccccgatgac
    16201 atctagatag acattgtaaa gggttgttgc atcttcagga tgtagatcag atgctgtgct
    16261 gcttggcaga acaggttcta cccatttttt gatgtacgcc ccgttataat ccaagtaact
    16321 gttttcaaaa ttccgttcat ttccccaaag ctgaacggtt tgagctggaa cgtttctatc
    16381 ttcttcgcct gcagttgatc cattattagt agcatagtcg taatttcgcc agttcgctcc
    16441 cccgtttgta taatcactat catcgatcaa gttctctccg tcatatggcg agcctttgtc
    16501 tccttgatag cgttcattgc tttgtgtcca atttcctaac acttttgtat agtcattcgt
    16561 tgtaaacttc gggtagcgat gctgttcgtt ttcaacttct ccgctaggat agatatattc
    16621 ataggggtct tgcgtagctg ttggcccaag acgctccaaa ataccgttga ataactggcg
    16681 ttgaatgcgc tggagtgctt gaccgctggg tacgattggt tcgtccagct tttccatctc
    16741 attttcttcg ctacgttgaa tttcagcgga atcttctgta tcagtttcat tttcttgtaa
    16801 ttcttggtta tctattcttt catcaaccac cacttgaaga ggtagttacc cttcgttcac
    16861 atcggggatc ttttctgttt caaaaatcaa tgtgccggtt tctgcttctt ttgaataaac
    16921 ttttccgaca taccattctt gaccatcgat cgtctcgact tttaggtctt ctccatcttc
    16981 atcataggag gatagacctg ttccacgaac gttttttacg gtaccaatcc cggcggctgc
    17041 cgcatcgatt gctaaacgta ttctaccttc attggcactt tccttttttt caaaagccag
    17101 atgccattca agggtttctt ctttttcaat atacttccct tcaatttttg catagtcctc
    17161 atctaagaat acctcatcct cctttgtttc tgttgactca gcagctacat ccagggtcgt
    17221 catatgatag actgactgga aatatggtcc aaatagcaaa ctgacagtta ctaactgcaa
    17281 aagcttcaat tttaaccctt tcacatcctt atactccttt caaaaatatt tgtcttcttt
    17341 cacaacaatc aacaaaacaa tgcccatcta attataattt aatttaattt taattttaaa
    17401 tcaaatgata tcctttttag ttaaaaaaca ccaattatac aacatttatt attaaaatta
    17461 aattataaaa ttttataacg ttctcattta acaaaactta agttattaag acaaatgaat
    17521 aaacaactac gctttcctcg aaacaaaaaa ataaagcaca aatgcgcatt tacgcactca
    17581 tgctttattc gtacaattaa ttcagataac ggcagcgcat aggatgccaa taacacttcc
    17641 taaaatagct cctcctaaaa tgtcagtagg ataatgtaaa agtaaaaaaa tcctcgaata
    17701 agcgatcaaa caagcaaata aaaaggctaa tattcctgca actggactta attcgaacaa
    17761 cgcagctact gataaaaagg ctagcgctgt atgtcctgaa ggaaaagaag aagtcttcgg
    17821 tttacttgtg taaaggacaa gccgacgatt tttttcataa ggtcttttcc gtcttgtcgt
    17881 ttttttgata aaaagcaaac caacaaaaaa atagatcaaa ctgccgataa gaatggctag
    17941 accatattct ctagtatcag gtatcaagac taaaacagca gcaaggagca accaaataaa
    18001 accaccatta gagatattac tgataaattt caaaaaattg atttttcgac tatccgtttt
    18061 ccgttgcttg gcgaacttgt tgataatact atcatcgatt ttagcaatca aacgcaacat
    18121 gttattcctc ctttttaaga accttttctt gcgtacgatg gataaaaagc aggacttgaa
    18181 aataaaacaa gaaactgaaa cctaataaag caaaatagat tggtcctgca aaccagaatg
    18241 cgacaagaag gatcaacgtg cctgccaaat agaaaagcgc gcttttgaaa tcattaaaaa
    18301 acaagaaaaa tgcagtaatc caagaagctt ctttttgttc cagttgaagc aagattttgt
    18361 aactaaagag aagcgcgctt aatagaagca aaaacaacgt agtatacatc aatgtatagc
    18421 caattccccc accccaatag cggaaaatct gataatcgat caatagaaga agaaaataaa
    18481 cacttgctaa actatcttgc tttccataaa gagagaagtt ctcacgaaac tttttccaga
    18541 atagtgaacc gagctttttc tcttcatttt ttttgtaatg ataaatcata tacagaactg
    18601 ttcgacttgc agttcctaat gtgaaaacag gcaagacgca caggaaccat agtagctgta
    18661 aatggataag acgtggaatc cattcaatga tttctaagaa ataatcattg gcttttttac
    18721 caatcgatcg attcatcatc cttttgaacc tcctgtcaaa tccatccctt caatgaaata
    18781 acgttgtgca aaaaagaaca gcaaaatcgt cgggatcaag acaagtgttg ctcctgccat
    18841 cagataattc cattgtgtcg ttgcttggtt ctgaaaaact tgcaagccaa tctgtaacgt
    18901 gtatttttct tgatcttgga tatataacag aggacctaag aaatcattcc aagctgcgtt
    18961 gaaggaattg atcgcaatgg taatcaaggc aggtttcgtc aatggaagca tcaaatgact
    19021 ccaaatatac aaatgattcg ctccgtcgat ttcagcagct tcgatcaatt cattattgat
    19081 cgataagtaa aattgacgca tcagaaatat attgaacgca ttgccgaaga acgatggaac
    19141 gatcaggggc aaataagtac ctacccagcc gatcttggag aataatacat attgagggat
    19201 catcgttaca aaccccggga tcatcatcgt agaaagaacc aaagcaaaca tcagcttttt
    19261 cccaggaaaa tcaagcttcg caaatccata agcaataaaa gaatttgaaa ggacattacc
    19321 gatcactact agcacagtga tgaataatgt atttcttgca tagcgccaaa aaggaaaggc
    19381 ggcaatcgta tccagatagt tttcccatac gattttttct gggaaaaagg tcggaggata
    19441 acgaacgatt tcttccattg gtttcaatga agtaaacacc atccaaagca atggaatgat
    19501 caaaatgatt cctattactg tcatgaggac ataacttagt attttttttg tttgccgatt
    19561 cacactggtc tttttgaaat aatccatcat ttgccgtcac ctccagaata atagacccac
    19621 ttaggtgcaa gcttcaaatt gacaaacgtc agaaccaaca cgatcagaaa caggatcatt
    19681 gacatagcca tcgcataacc catatcaaaa gctacgaacg ccttgttcca catatgcaga
    19741 ttataaaaaa gcaaagaatt tgcaggtccg cctgttccat ttttcgtcat gacataagct
    19801 tcttgaaaaa tttggaaaga accgatcgta gaggtgatca catcgtaaaa aataatagga
    19861 gtgattaatg gcaaagtaat acttttgaac tttccccaaa cgcctgctcc atcgatttct
    19921 gctgattcat ataaagatct aggaacattt tgtaatgtag ctaagtaaag tagcattgct
    19981 cctccagaac tccatatttt catcaaaatc aacgcaggtt tcgtccattg tggatcaaac
    20041 agccaagctg gtccagggat atggaaccag cccagaaatg tgttgatcaa tcctgcagat
    20101 ggagaaagca gctgcatcca taagatgtag actgctactc ctgataagac tgccggtaaa
    20161 tagaaagctg tacggaatac actgattcct ttgatttttt gattgagtac tgtggctaaa
    20221 aacacactga aaatcgtcgt cagcggtaca ttgaagatga cgaaataaac ggtgttgtat
    20281 aacgatttcc aaaataaatc atcgtgaacg aacattcttt tgaaatttgc tagaccaatg
    20341 aaattcatct tggatgtcaa attataatcg gtgaaactac caataactga aaaaagcata
    20401 ggccctagca taaataccaa gaacccgata ataaaaggcg agataaataa ataggcccat
    20461 cttgcttctt ttttctttcg gatagataaa cgcttcaaat tttcttgcct cctctgtacc
    20521 tagacagtca aaaatggatt attgattttg cttgactaag ttttctactg ctttttgtgc
    20581 atctgccaat ccttcttttg gtgattttgt tcctaaaaga atctggtcaa tctgttcatt
    20641 cactaatgtc gtgtaatccg gagcatctaa aggaactggt gtgatcaccg tatttttaaa
    20701 gttttcgtcc gccatttgat agatcatttt tcctttatcc gttaggttgt cattattcac
    20761 taaattctca ttggctgtct tgctcgccat gatatcaaaa cttttttcgc cgaatttttc
    20821 ttgtacttct ggagaagaaa gatatttgat gaattcataa gaggcctcag gatcttttgc
    20881 tccgtatggt acttccaaga cgaatccgcc accccacgac caatggcctg aaccttcttc
    20941 tttttctggg agctgcatca cgccaaaatc aaaatcatcc ggtgcatttt ccatcaaact
    21001 agagtaataa ttgatatttt gggcacgcat ggctactaat ccggaaataa atggatcaga
    21061 tacaccagac ccaaattcgg cttctaattt attgatcgta tttttcccat aataatcttg
    21121 ccagtcttgg atccactcca aggcttccac tttattttta gtatcaatct tgactttttc
    21181 atctttgtca aaccaactag tcccgttatc tgcgtttagc gcccatacat cagcccctaa
    21241 attccataat ggataaaaac cgatgcgtgt aaattctttt ccttcttttt tatctagttt
    21301 acgtgctgtt tcttctaatt catcccaagt tgtcggtaga tcttgttcac tgattccagc
    21361 ttctttaaat aactttttat tataaaaaat gacttgtgta tctgtattga atgggatcgc
    21421 ataaggatca ccttcatatt ctactgtatc ccaaagctgc ggataaaaat ccttagaagt
    21481 gtctttttct acatatttgg agagattagt tgcttgctgt gcttctgctc tttgggcaac
    21541 ggaatttatg tcctgtacaa taacatcggg tgcatttcca gctgttattg cagataaaga
    21601 tttagtccag atgtcacccc atggctgata cttataattt acatggatct cgttttgtga
    21661 ttgattgaaa tcctcaatga tttcttcgat agcttctttt cgtgcaccgg atccccaaaa
    21721 ggaccagaaa tcaatatttt ttcttccatc acttgttttt tcagaagaat ttgctgcact
    21781 tgaactggca tcgcttgcat tacttgatcc gcatcctcct aataaaaaca atgctgaact
    21841 tgccagcaac cctgtaaaca ataatttttt catattttac ctccaaattt taatgtaacc
    21901 gatgccggtt ttaatataac gatatcattt tgttctgaaa ataacaattt ataaggtatg
    21961 attctaaaaa aattattaat gataccaata ctttatataa ttcgcgagcg ctttctttga
    22021 attttttaat ctttcgtagc agaatgagaa tgtattaaaa atagattagg aggaattttg
    22081 atgtttagac accagaaaga attgcagttt gaagcaaaac cagatcgtcc gaatccatta
    22141 atcgcgaaat atctgcagga gctgatcggc ggccaatatg gcgagatgag tgtagctatg
    22201 cagtacctgt tccaaggctg gagctgccga ggagaagaaa aatataaaga tatgctgatg
    22261 gacattgcaa cagaagaaat cggccacgtg gaaatgttgg cgacaatggt tgctcgttta
    22321 ttagagggtg cgcctgttga agatcaagaa gcagcgatgc aaaaagatcc aacaatcggt
    22381 gccattatct ctggaatgaa tccacagcac gcgatcgtag ctggcttagg acctcgtcct
    22441 gctgacagcg tgggcaatcc atggagcggt gcctatatca ttgcgagtgg caacttgtta
    22501 gcagacttta gagctaattt aaatgccgaa tcacaaggaa gacttcaagt agcacgtatt
    22561 tacgaaatga tcgatgatcg tggcgtaaaa gatatgctgt ctgttctcct tgcaagagat
    22621 agctatcatc aaaatctatg ggaatgtgca gtgaaagaat tagaagaaaa agaaggttca
    22681 atcttagtcc caagtacttt cccgcgtgac aaagaacgaa aagacattgc ttatgacttc
    22741 tataacttct cagagggaga ccaaagtagt gaaggttctt gggctagtgg caaagcactg
    22801 gatggcgaag gcgaatatca atggttgaaa gaacctaaga tcgaaggcgc tgcaccaaaa
    22861 ttagaaccag taacaccaaa aatgtatggg acacctccat tcaagaaata aatatctacc
    22921 cgtagaaaca cccgtttttt gttccacacg ctttgtgagc aaaactgact aaagtcatta
    22981 atcataacca cccttcaaaa gggtggtttt ctctagagct ataagctcaa atcaccggcc
    23041 agcgcctaaa gacgctggct ttcatatttt atgttcaagc catattgcct ttgccagttt
    23101 tgcccacctc ttaaaaggtt tatgtactac tccttagccc ttaaaaggat cgtcttactc
    23161 tcgtacactc agcttatcta acgcactatc atgtttttct tgttcttgaa tgtacttttt
    23221 gatggtcgct tcattttgtc ctacttttgt cacatagtat ccttccaccc aaaagtgtcg
    23281 atacccaaat ttatatttta gatttgcaca atctcttgat gatctcctga atattggctc
    23341 gatattgatt ataaattatt tttcttctat actttggtgt gaatacaatg tggtatttac
    23401 acatctactt tgtacgggct aaactatttc cctttattgc tccacccgca tagcaagtgg
    23461 ttttgtattt caatcgctcg gcgcgctcaa ttaggtctaa agacaataaa tctaaaaacc
    23521 cgctgcttca ctgacagaaa caacgggttt tcagaaaaaa atgaaatgaa aaagatctct
    23581 tacggcaatc tattttattt ttttgatttt ttataaatgg aaaatgcttc aagaagtcca
    23641 agaaataaag ccgtataaaa acaagaacct atgaaatctt tatttattag atattcaaca
    23701 gaaatcccga taacactagc caacaatata tttatcagaa gtgtaaaata aggatgcttt
    23761 ttatagaata gaactagtgt tgttttcata gactctcctt tcttcatatt tccaattaat
    23821 cacaaattca cggcattact agaaaaatta aaggggcgca gaagtggaaa aatactcttc
    23881 gactttccct acaaaccagg ccatcttgtg tttaataact ataccaaatc taaggtacct
    23941 ctaaatctaa attccatttg tggcggtgac ctccaatttt taactcttct atctcccatc
    24001 cagtatacat aaccttgata cttcgaccac ttatatatcg aacacacaaa tacctatcca
    24061 ttgcaggagt actacctaac ttagttgcta cttgcatcta aataacagct gaaaagatag
    24121 gacttactct tagtaaagta tctgaactat taatttttaa actatcattt ttatcctcaa
    24181 ctacatgcac atctgttgca acttcacttt ctgtctcatc cagataaatg attaaggaat
    24241 cgggaattgc aatatcttca gctgacgctg gaatagttga gctaaattag tcatcatatc
    24301 tttattataa aataaccaac agcaaaaaat acaatgaaaa acaaacttgg aactaatgta
    24361 tcattaataa atgttgaaaa attcagcaac tcattactgt atgattttag cagcccaacc
    24421 aacatatacc ctataaaagc taataagatg gttaggaatc ctttagaatt tttactcatt
    24481 catagacact tccttaaata acttttatta tagaaggtat tgtaaataat aatattgaca
    24541 aaaagtgact caaaataaaa aaaaccttac attatactgg aatgaactgt attgatagtg
    24601 tacctaaaag ctctactgac ataagatcgt gtaatcggta aagtaatttt attaattata
    24661 cgggatgtaa tcaattagag aataaactaa tcataaaagg tacttttaca ttcataattg
    24721 tcgctttgag attctacttt ggttcactat ctgacccaat tgcacatttt cgatcattac
    24781 aagtattggt gtcgttagaa caactaaata aaaactagcg gtagattttt cagaatctat
    24841 gctaatttct gtatggatgt tccagtgatg gcaaatttct cttttcattg tttttcttgt
    24901 cgcatgatat gcctttttgt gttattcagt taaactatag aaattctttc ctagacagat
    24961 cttgatccca aattttacgt ttttgagcat cgatatagtt taaaatgaca ttactcatat
    25021 ctaaagagtt tagatttttt aggactaaaa ctgtcagttg ttctttttct tctttctgtt
    25081 aaagtattgt cgcgactttt cgtatacgtt aagatatgtc gtgaaaaaac aagttttgtg
    25141 acacaaaaaa acgaatgact tgaggaaatc acctcaatca ttcgtttaaa atgctatata
    25201 ttctttagtt taagatacgg aaattaatgc catcgaccac actagtagaa tgaacgacat
    25261 ttacttggcc tttttttaat ttcgttatgt ttagcatata aaaatattat agagtttact
    25321 tacaatgctt aaaacatata ctgtataatc tgatcaattt tagccatgtc ttgcaatggt
    25381 aagttttcta tttttttgag ttttcgttcc ttaaaatcaa gagattttaa ttgtgtaata
    25441 attacttgtc cttttatatc tagctcttca ggtaatgagt aacgtgtcgg taaattcttt
    25501 atagtggaag taattggaca tatgaccgaa aacattgttt tacggttaaa ttcataacga
    25561 gagacgacta gtcctggtcg tcttttttga atttcttttc ctacggaggg atcaaaatca
    25621 atccatacaa tatcaccttt tttaggaata taaattcttt cttctgacac tataaaattt
    25681 ctctcccttc aggagttaaa tcttcccatt cgtctttctc ataatactcg gcctcttccc
    25741 caccactaaa tggatcttca atttttggaa ctaacgtaat tgttccatca tcagaataca
    25801 caacgatata ttcttggttt tctgaaggtt tctttccgtt atcagaaggt aaagttacca
    25861 ctacagaact tccttgtaat ctagattttg taacaagcat tatcatcact cccttaataa
    25921 aagtatacta tataacacta agtaacgcaa aattcccctt tgtaaactat ctagaaaatt
    25981 gttttgttta aagtatatct tattggatat aaatagaaat aaattgtagc cagttcattt
    26041 tttctttaac taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca
    26101 aatactatag ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa
    26161 tttcaaagac aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct
    26221 tttgctctga caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa
    26281 ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta
    26341 gacaagactt tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt
    26401 tacatgatcc aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag
    26461 aatatacaac tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt
    26521 cataatgata aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa
    26581 atagaagcta tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta
    26641 tgagcatttc ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt
    26701 gccgctcttt ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc
    26761 tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct
    26821 tcagtcaaga caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta
    26881 gtcattatga ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata
    26941 tacactattt tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcattg
    27001 ggaaaacaca acgattttgc aaaaatggtt gatgaaaggt ctttttttct tctactgtct
    27061 ctgcacgggc attaatcatc aattgtcctt ttttaaatcc ggtaaatccc cacttcatga
    27121 tttccggagt gaggatgttt tcttcctctt ttcttaacgt cacaacaaga tttgacggaa
    27181 aaacttctcc agctgctaat tctgctgcct gcttcggttg tttcgtctgg gcttctcgat
    27241 aataattctg aaggagctct gactctagat caaaaaaata acgaccacac atcctaatcc
    27301 ctcccaatct ttttttctat catacactgc aacaagaagt tcgcaccaac aaaacgcttt
    27361 caatttttgt agaactaaaa aagttgtgac aagtgacaaa ctctccagtg aatggtattc
    27421 tcctgccaac tcctcggtca caagtcacaa tattcaacaa acatgagaag ctgctttttg
    27481 aaattgttcc ttgtgactcg gagtgaaacg gcaggcttac gacctctggg taaacggtgt
    27541 tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtagaa agctattttc
    27601 acaaaatttt tcttaatact cgaaactaag cgactttttc acaacctctt ccttaaacgg
    27661 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gagcagctat
    27721 ttttatttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tctccactaa
    27781 acggtgttca atcagctaac cttcggtatt agctcaaaaa acggcaaaat atgaggaact
    27841 attttgccgt ttgtttctct aatactcagg tcaaagcgct gatttcacaa cctctaatac
    27901 acggtgttcc agaagtagct tcttcgaaaa ataagccgaa atttcacaaa aatttgagaa
    27961 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca caacctctaa
    28021 ttattaaaga accttttgcg aagttaacaa tctttctttg atcaattgaa aattctgcct
    28081 aagtagtgat agatcctgtg tgactttcaa caataagata cttccctgga tttcagcaat
    28141 caacgctttg gcttgacggt ctgcttcttc atcggacagt ttttcttcag aaagtgccag
    28201 gatattttgt ttcgtatact tgacccattg attgactaat tctagtagca acagacggta
    28261 ttcttcatcc actgtactca actcaataat caaattacct acaggacagc catgataagt
    28321 gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt tcttctaatg cttcttctgg
    28381 ctgttttcca gcatttaaga tgtcttcgaa caattctgtc tgccatacgt ccatatgcgc
    28441 atggatcacc gctagacaaa cttctttttt tgaagaaaag taataataaa attgaccttt
    28501 tcctgtatca gaagctgcta gaatatctcg gattgacgtt tcatgatagc cttttttata
    28561 aataagatct ttggtcgctt ccaagatctt ttcctttgtt ttcaatgata ttctcctttt
    28621 tcatttctca tagtcctata ttatacagta aattcatcat tttttcttct taatcgttta
    28681 aaaaagactg tgccaaatta tctgacacag ccctcactat aaaactgttc tcatatagct
    28741 agatgttcat tgattcaaga actgcgagga caaaagcaat tcctatctta tgatagttgc
    28801 tctaatactt cctctgttgt catcacttga tctgcaataa agccaaagtt cgtcaacgct
    28861 gcatccattg cttcttggcc aggagcacca attgcatctg tcgctaccac aacattgaat
    28921 ccactctcta ccaattcacg catatgggaa tcgctacaca agtttccgtt cattccacca
    28981 agaattactg tgtcgattcc attttttctc aattggagcg ccaaatcatt gctttctggt
    29041 ccaaagattt tatgaggaga gctgatgatc gtattttcat ccaaataagg tttcaattct
    29101 tcgataaaat cggctcctga accttcttcc actgattcat attgactttt tctagcaaac
    29161 atctgctgct caagcatcat atgttctcct gcaccgccaa attgccattt atggtcatgt
    29221 gggtaaaaat aatgaggaga aataaagagt ttgtatcctt tttcttttgc ttgttttacc
    29281 atttcaatca tattttcgat cgtattgtat ttttctaaga tatcttttgt caaaccaaag
    29341 ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag tgagaactac tgctgtattt
    29401 ttattattga acatggtgga ttctccattc gttatttatt ttaatacatc atttagagct
    29461 tcactcattg tcggatgaga atagatttga tctctcaata acgtataagg aagttttcca
    29521 ttcatagcta gcgaaatcaa gttgatgact tcatgagaat cttccgcata gatacttgcc
    29581 cctagaatca agtcagtttc agggtcgact aaaactttga atacaccttt aggatcttct
    29641 aatacttgtg ctttagggac accgcttgtc atatacttga ataatttgta attgacgcct
    29701 tgtgctttgg cttgtttttc tgttaaacct acattggata aagtcggagt gataaataca
    29761 ctgtaaggca cagctttccg gttattcgtt gttcgtttat tctcgccctt caactgatcc
    29821 ataatgattc gataatcatc tagagagata taagtgaact gcaatccgcc tttgacgtct
    29881 ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt catccacagc gattgctccg
    29941 cgatctgtta gtttaacatc tgtattttct aatccaagtg atgctgtact tggttttctt
    30001 ccagttgcag ccaaaacttt tgacgcttgg attgttactt cttggccatc ttctgtaaag
    30061 gtgacggcag ctgttgattc ttggtctgct acttgatcta cagatacacc tagatggaat
    30121 tcgattcccg catttgtcat gtcttctaaa atcatttgcg cgatatcttc atcttcacgt
    30181 ggaagaaatt cagaatgggc atcaagaacg acaactttag atccgtattc attgaacatt
    30241 gatgcaaatt ccaagccaat atatccagcc ccgatgatca ctaatttttc cggtaattgt
    30301 tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac ttgtattcaa tcctttgatt
    30361 ggtaaaatga ttggttcaga accagtattg atgaaaatcc gttcgccttc aacaatcact
    30421 ttttcaccat tattcgtcac ttcgatttga tggttagaaa gaaaacgtgc tgtaccgtcc
    30481 atcactgtcc cagttgcctc atcgctgatc atatgataat ttttggcgcg aagcatgccc
    30541 gtcaattttt ctttccgagc aacagcctct gtaaagtcga ttcctctttc tccattgaaa
    30601 atcaatgatt tagaaggaat acagccgatg ttgatacatg ttccgccata catacgtgtt
    30661 gacttttcaa cgactaaaac cgattcacct tttgaagcta acgccttcgc taaagtctta
    30721 ccgccctttc caaaaccgat cacaatattt tgatattttt ccatcatttt tccctcgctt
    30781 cttttttacc taccgattgg tacaaaaata tgataaccga taattgtcta ttctccaaat
    30841 catctgcttt atttgtcaaa aa
//
LOCUS       DAFCNS010000035        29110 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFCNS010000035 DAFCNS010000000
VERSION     DAFCNS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.035"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1030)
                     /locus_tag="LNE23_001450"
     CDS             complement(<1..1030)
                     /locus_tag="LNE23_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6931308.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="LNE23_001451"
     CDS             complement(1112..1801)
                     /locus_tag="LNE23_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK6931309.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="LNE23_001452"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="LNE23_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBK6931310.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="LNE23_001453"
     CDS             complement(2328..2831)
                     /locus_tag="LNE23_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBK6931311.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="LNE23_001454"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="LNE23_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBK6931312.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="LNE23_001455"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="LNE23_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBK6931313.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="LNE23_001456"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="LNE23_001456"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBK6931314.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="LNE23_001457"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="LNE23_001457"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBK6931315.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="LNE23_001458"
     CDS             complement(6443..7462)
                     /locus_tag="LNE23_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK6931316.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8540)
                     /locus_tag="LNE23_001459"
     CDS             complement(7635..8540)
                     /locus_tag="LNE23_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6931317.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="LNE23_001460"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="LNE23_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBK6931318.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="LNE23_001461"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="LNE23_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBK6931319.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="LNE23_001462"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="LNE23_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK6931320.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="LNE23_001463"
     CDS             9753..10397
                     /locus_tag="LNE23_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6931321.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="LNE23_001464"
     CDS             complement(10453..10956)
                     /locus_tag="LNE23_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK6931322.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="LNE23_001465"
     CDS             11553..12518
                     /locus_tag="LNE23_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK6931323.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="LNE23_001466"
     CDS             complement(12617..13789)
                     /locus_tag="LNE23_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931324.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="LNE23_001467"
     CDS             complement(13776..14921)
                     /locus_tag="LNE23_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931325.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="LNE23_001468"
     CDS             complement(14935..15867)
                     /locus_tag="LNE23_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931326.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="LNE23_001469"
     CDS             15984..17063
                     /locus_tag="LNE23_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK6931327.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="LNE23_001470"
     CDS             17060..17689
                     /locus_tag="LNE23_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6931328.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="LNE23_001471"
     CDS             complement(17799..18026)
                     /locus_tag="LNE23_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6931329.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="LNE23_001472"
     CDS             complement(18129..20717)
                     /locus_tag="LNE23_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBK6931330.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="LNE23_001473"
     CDS             complement(20737..21396)
                     /locus_tag="LNE23_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6931331.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="LNE23_001474"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="LNE23_001474"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBK6931332.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="LNE23_001475"
     CDS             complement(22169..23035)
                     /locus_tag="LNE23_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6931333.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="LNE23_001476"
     CDS             23144..23785
                     /locus_tag="LNE23_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBK6931334.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="LNE23_001477"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="LNE23_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBK6931335.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="LNE23_001478"
     CDS             complement(25226..26125)
                     /locus_tag="LNE23_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK6931336.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="LNE23_001479"
     CDS             complement(26127..26924)
                     /locus_tag="LNE23_001479"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBK6931337.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="LNE23_001480"
     CDS             complement(26902..27606)
                     /locus_tag="LNE23_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBK6931338.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="LNE23_001481"
     CDS             27737..28312
                     /locus_tag="LNE23_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBK6931339.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="LNE23_001482"
     CDS             28430..29107
                     /locus_tag="LNE23_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK6931340.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6169 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaaa
//
LOCUS       DAFCNS010000036        26816 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFCNS010000036 DAFCNS010000000
VERSION     DAFCNS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.036"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..411
                     /locus_tag="LNE23_001483"
     CDS             <1..411
                     /locus_tag="LNE23_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK6931341.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="LNE23_001484"
     CDS             509..697
                     /locus_tag="LNE23_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBK6931342.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="LNE23_001485"
     CDS             complement(755..1108)
                     /locus_tag="LNE23_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6931343.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="LNE23_001486"
     CDS             1639..2061
                     /locus_tag="LNE23_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6931344.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="LNE23_001487"
     CDS             2058..2288
                     /locus_tag="LNE23_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6931345.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="LNE23_001488"
     CDS             2786..2986
                     /locus_tag="LNE23_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBK6931346.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="LNE23_001489"
     CDS             3013..4206
                     /locus_tag="LNE23_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6931347.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="LNE23_001490"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="LNE23_001490"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBK6931348.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="LNE23_001491"
     CDS             complement(5898..6821)
                     /locus_tag="LNE23_001491"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBK6931349.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="LNE23_001492"
     CDS             7017..7622
                     /locus_tag="LNE23_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6931350.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="LNE23_001493"
     CDS             7930..8946
                     /locus_tag="LNE23_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK6931351.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="LNE23_001494"
     CDS             9087..10388
                     /locus_tag="LNE23_001494"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBK6931352.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="LNE23_001495"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="LNE23_001495"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBK6931353.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="LNE23_001496"
     CDS             11117..11515
                     /locus_tag="LNE23_001496"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBK6931354.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="LNE23_001497"
     CDS             11581..12663
                     /locus_tag="LNE23_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6931355.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="LNE23_001498"
     CDS             12875..14437
                     /locus_tag="LNE23_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931356.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="LNE23_001499"
     CDS             14430..15563
                     /locus_tag="LNE23_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931357.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="LNE23_001500"
     CDS             15563..16519
                     /locus_tag="LNE23_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931358.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="LNE23_001501"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="LNE23_001501"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBK6931359.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="LNE23_001502"
     CDS             17808..18626
                     /locus_tag="LNE23_001502"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK6931360.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="LNE23_001503"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="LNE23_001503"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK6931361.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="LNE23_001504"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="LNE23_001504"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK6931362.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="LNE23_001505"
     CDS             complement(20506..21087)
                     /locus_tag="LNE23_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6931363.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="LNE23_001506"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="LNE23_001506"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBK6931364.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="LNE23_001507"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="LNE23_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBK6931365.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="LNE23_001508"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="LNE23_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBK6931366.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="LNE23_001509"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="LNE23_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK6931367.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="LNE23_001510"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="LNE23_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK6931368.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         8434 a         4951 c         5684 g         7747 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc tttttt
//
LOCUS       DAFCNS010000037        25855 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFCNS010000037 DAFCNS010000000
VERSION     DAFCNS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.037"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1182..1847)
                     /locus_tag="LNE23_001511"
     CDS             complement(1182..1847)
                     /locus_tag="LNE23_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6931369.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2027..4009
                     /locus_tag="LNE23_001512"
     CDS             2027..4009
                     /locus_tag="LNE23_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBK6931370.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4013..4651
                     /locus_tag="LNE23_001513"
     CDS             4013..4651
                     /locus_tag="LNE23_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931371.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4679..5107
                     /locus_tag="LNE23_001514"
     CDS             4679..5107
                     /locus_tag="LNE23_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931372.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5564..6475
                     /locus_tag="LNE23_001515"
     CDS             5564..6475
                     /locus_tag="LNE23_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK6931373.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6692..7129
                     /locus_tag="LNE23_001516"
     CDS             6692..7129
                     /locus_tag="LNE23_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931374.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7224..8108
                     /locus_tag="LNE23_001517"
     CDS             7224..8108
                     /locus_tag="LNE23_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK6931375.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8332..11499
                     /locus_tag="LNE23_001518"
     CDS             8332..11499
                     /locus_tag="LNE23_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931376.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11519..12064
                     /locus_tag="LNE23_001519"
     CDS             11519..12064
                     /locus_tag="LNE23_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBK6931377.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12092..12547
                     /locus_tag="LNE23_001520"
     CDS             12092..12547
                     /locus_tag="LNE23_001520"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBK6931378.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12544..13302
                     /locus_tag="LNE23_001521"
     CDS             12544..13302
                     /locus_tag="LNE23_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK6931379.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13388..14128)
                     /locus_tag="LNE23_001522"
     CDS             complement(13388..14128)
                     /locus_tag="LNE23_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6931380.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14189..15442
                     /locus_tag="LNE23_001523"
     CDS             14189..15442
                     /locus_tag="LNE23_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK6931381.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15764..15991
                     /locus_tag="LNE23_001524"
     CDS             15764..15991
                     /locus_tag="LNE23_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK6931382.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16114..16440
                     /locus_tag="LNE23_001525"
     CDS             16114..16440
                     /locus_tag="LNE23_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK6931383.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16508..17221
                     /locus_tag="LNE23_001526"
     CDS             16508..17221
                     /locus_tag="LNE23_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBK6931384.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17531..18382
                     /locus_tag="LNE23_001527"
     CDS             17531..18382
                     /locus_tag="LNE23_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6931385.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            18503..20368
                     /locus_tag="LNE23_001528"
     CDS             18503..20368
                     /locus_tag="LNE23_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6931386.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20416..21825
                     /locus_tag="LNE23_001529"
     CDS             20416..21825
                     /locus_tag="LNE23_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBK6931387.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            21933..22634
                     /locus_tag="LNE23_001530"
     CDS             21933..22634
                     /locus_tag="LNE23_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6931388.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22793..23272
                     /locus_tag="LNE23_001531"
                     /pseudo
     CDS             22793..23272
                     /locus_tag="LNE23_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            24120..24695
                     /locus_tag="LNE23_001532"
     CDS             24120..24695
                     /locus_tag="LNE23_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBK6931389.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            24695..25135
                     /locus_tag="LNE23_001533"
     CDS             24695..25135
                     /locus_tag="LNE23_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK6931390.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            25151..25852
                     /locus_tag="LNE23_001534"
     CDS             25151..25852
                     /locus_tag="LNE23_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK6931391.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         8728 a         4199 c         5342 g         7586 t
ORIGIN      
        1 ctaatatatt catatgagct aattttatga gtaaatggta agtttttcct tgtttgtttt
       61 agtttaaact agtgtgtaat ttaaaaatct atatggaagg gtctgtgggg taatacttaa
      121 ataaaggcta ggaacctgat aacatcaagg ttcctagcct ttttataata aaatgaagcg
      181 aaaataattt cttttaaaga gccgagatta tcagctatat aagagtgaat ttgatgcaaa
      241 aaacagagcc gaaacagtct tggtgttcgt agctctgctt atatgtttcg aatttaagac
      301 aaagagttgg caaaagatgt tgataaggaa gataagataa ctgtgttata taaactaggt
      361 aactaactgt aacaagctaa ttttaagaga ttcttattag aagacgttta ggaatataac
      421 taagaaaatg attatacagg taattgtcat ttcaataatg ttttctaatt ttcgcgtctt
      481 tgttttaaga aaaaggccag aatcagttgg aaaaaagttc gtacgatagt gaagaggggc
      541 aaaatttcct actaatattc ccactaacaa gagataaaac ggccaggttg gtgtgattcc
      601 tctataaaaa aatgcccatg ctaccgaaat cccatagatt aacaactgaa caatttttac
      661 aatgaaaaaa ttgttctgcc attactcaaa gtctcttcct ccttgtatcg tttatttgtt
      721 tcattttatc attatgatag gcgcttgaac aatccatttt gtattttatt gagtactctt
      781 ggataaacta ccagcgatta tcatatttat ttatgttact gtaagccatc atataaaagt
      841 aaatgagcag atcatagaaa tataaaaaag aactaaaatt gatctaaatt atgtgcagct
      901 tgactacgta ccaatctata ttacttacta gattggtcgt taatctattc ggatcgaatg
      961 agcaaaatat ttttgtgaat gtccatcctt gattatgatt ccaaaaagaa tcattgggag
     1021 gctgtggcgt aaatattata ctataagcca gaaataccta tcattctgaa cttttttatt
     1081 taggaacttt catctcaagg ctttttcaat taatttgtac ccctgttttt tggataagat
     1141 catgtgcatt gattttttct atctctacgt tcatctccta cttaatgcct atctggctcc
     1201 tgagggcttc tttttaaggc gtcgtcatag tcttggctga ggatttcttg gtaatctgag
     1261 ggaatggctt cccaagtgag caggcgaacg ttcatgacgt gttttccttt gtgtttgatt
     1321 acagcatggt ttttctcttt ttctacctct ccagcgtgaa aagacagcca tgtaccatgg
     1381 ccaaagatat cattacatac ccaacgttcg atggaattgt ccacgtatcc tacagggagt
     1441 gtggtatagc cgatttgtgg tttcaaaaaa ggattgaatc gatcgatcgt ttcatttttg
     1501 tattgataat tgataagaaa aagatagacc aaacaaaaga cgagcagaaa gaaagatagt
     1561 gtctttttga gtgagaaggg ttgttttgat gaataagatt tcttgttaac aagaggttca
     1621 tctggcaatg ttggttctat tgattcggta gctgattcaa tagaaggaaa atcaatcggc
     1681 agacctaaag tatgatgtaa aagtgacagc atttcaaggt ctggcattgt acgagaattt
     1741 tcccaactag aaatcgtttt atcggaaaca ccaatttttt cgcctaattc tttttgcgta
     1801 agcttacaat ttctgcggta agttcgtatt ttttctccgg tgttcatctt cttctccttt
     1861 cgattctaca gtatatagat ttattctaca tgacctatac acttgataga tacgtttttt
     1921 tgatgaaaaa cggtatgatt ttatcaaaga atcatttaag taagggagag tggtgataat
     1981 caaaaaaaag ttcacagttt tactcacaat catagtgacc ggtatcatga gtggttttgc
     2041 cttggttagt ataaataaac aggtactcta tcggacaaat gaatttttcc atcaaacaga
     2101 tacttatact ccgattcgat tgccacaaca ttttattggg caagaagaat atgagcagct
     2161 aatcatggta tttgaaaatg tctcatcaga aactgagctt tcttatatga aaaaaacagt
     2221 aaatgaatcc aatgaagtag atacatcttg gaacttcaag atacagccaa agcttgaaat
     2281 gacattgtat tatcaagggg atgtcaaatc ttctcacgag aaggtaccct attctaatac
     2341 ctcctttcgc gtaacgtcca ttgaagaatc gatcaaaata aaagattttg aaggtgaatt
     2401 tttcttgatg acgagagaaa aagaagccta tgaacggttc atttcactat tcactgatcg
     2461 attcaatcaa gcatttcaca ctgattatgc cactaatatt tttgaagact ttgaaaatag
     2521 ctattcgtat tcggttattt caaatacaga cgtatataat tatcgagtgt atttaactat
     2581 tggattgatt cttttattcc ttttgttatc atcctttttc ttattgatga ataaagaaat
     2641 cagtttattg ttgaataatg acttttcatt acatcaagta ttgtggtgtt tattggggaa
     2701 aaagaaaatc attattacat tcataattgc aggaatcatt ggtggtttta tttttatgta
     2761 ttataaggag caatttcttt atttgttcca gatgtacgtt ttcttgttca gcggaatata
     2821 tggtatctct ttgatttctc ttttattagt tgaaaaaatc gcttcgttaa gagaagcgtt
     2881 caactatatc caatacattc tttttacatt tggattgttg ttttctcttc ttttgaccgt
     2941 tgccaatgtc gaattagcaa ctatcgtttt ttcttcttca cagttagctg tttcccatga
     3001 accaccaaaa gaaatcgctg gtgaggacta tcatgtattt tatccagtaa ccatcgggaa
     3061 aaaccacgtg gattttattt attccaatcg ttttgcatca gcagcagatc aggaattata
     3121 tgaaactttg aataaaaacg gaagtatatt agtcaatgta tcagattact tgaatgaaga
     3181 aaatgaacag tttggcagag ggataaaaat aaatctgaat tatcttaaga aatttccttt
     3241 gattgatgtc tctggaaggt tgatccaaat aaccgaaaaa gaaacgcacc ctgttatatt
     3301 gatccctgaa cgatatagac atacagattt aaaaaatgac ctggcgcaaa taacagaata
     3361 ttatcaagaa atcagtgaaa aagaaccgaa atttctattt ataaaaaaca atcaacccat
     3421 ttatacattt attccttcta tcccctggat cgaacattat ccggttgttg aaatactgac
     3481 attgaaaaac agtatgtatt tggaaagaaa tattctcagc ggagaaatct atccaccgtt
     3541 aaagatcaag ataggctctt tgtcgaaaga aaggttattt gagctgattg aagaaagtca
     3601 gttacgtgat aacttgcagt tatcatttcc gtatactcag actgaagaga tagctgtcaa
     3661 agtgttaagc agatcatttc attacttgat acagatattt ttactcactt tcttttcttt
     3721 cttcttgttg agctatgtca tgttatgcat atattttgtc tacaattgtc ggaaaatcgc
     3781 tatctttcgt tcaagtggtt attcgctctt tgaaacgtat aaagatttct tcatgatgaa
     3841 cctcatcaaa tggggaacga caagtgtaat cttccttttt cttatagaaa gagagcctaa
     3901 atatcttttt aatatttttt tctttagctt cattgatttt attctttcag ttgtttttat
     3961 tttgtctaca gaaaagaaaa gtcagttatt gctgatgaat ggaggttaac aattggagcc
     4021 gttgatagtg ataaaaggtg cgaaaaaaat cattcgaaac aaaacgattc ttcatttgga
     4081 tcgtttcact gcggatgctg gggatcttgt gactgttgtt ggagaaagtg gagcaggtaa
     4141 aagtacgtta ttgaatatat taggattgaa tgatcagttc agttcaggac aattctattt
     4201 gttcgatcaa gagacgaaac aattaactgg gagagagaag cgcttgataa aaagaaatac
     4261 catatctttt ctttttcaag attttgggtt gattgaatat gaaacaatcg attttaattt
     4321 agatattgga ttgaagtttt cgaatttaaa tgtttcagat aagcgaacag caaagtatga
     4381 ggcattgaaa aaagtaaata taaacaagaa gctaagtacg cctatctaca cattatctgg
     4441 tggtgagaag caaagaatcg cctgtgcgag agtcttattg aagaatagca aaatcatttt
     4501 agccgatgaa ccgactgggt cattggataa aagaaataag gataaagtag ttgaaatctt
     4561 atcgcaacaa gcttcccaag gtacgttagt tatcgtagtc acgcatgatc catatctgac
     4621 caaaatagga aacaagaaga tcatgcttta aaaatcaaaa aggaaaggat gtcgttttat
     4681 gaaaaaagca gttattattt gtgcactagg gataggtttt gttggaggaa acgctatgtc
     4741 atctgaagtg gaagcaaatt cagttcacgg tgaaatctat tacaaatctt taaagccagg
     4801 agagattcat tttgatgcta aaggtaatat tattaagtca aatttaccag tagaccggga
     4861 aatttctaga acagcaatta atcatagctc tggaagatgg gtttattata gtgatacaac
     4921 ttgtacgaga aaaacaggtc attctaacca ctatagtcgt gtctataatc gtcacggtgc
     4981 aaaagcgaaa gttggaaact ctattcgatc agcaaatgca acaaaaggag tatgggccta
     5041 tgcaagtgcg acaggaaata gaactgacac attctcatgt tggtacaatc caaccggttg
     5101 gtattaagta tccaaaagtt tatataaaat agaaaaaaca tataatgaaa cagcgttaaa
     5161 agttattttt ggtagtttta atatattcat ttttagttgt ataaaaatat atcataataa
     5221 aaacaacaaa aaaatgtctg gaaaatcgaa cataaaagtg tctgatttga atatcccagc
     5281 agcggaagaa gaaaaacaaa atcgataaca tttgttctaa gaattagtta cgttaaatac
     5341 ggaacgcaag atagacttga aaaacggtaa caaaagaagt agtatattaa ggtgtgatgt
     5401 gctacttgac atttattctg ctgagcgaac tttctggatt gtgtagcctt atatctattc
     5461 aggacgtatc gagcagatta gtaaaggtgt tcttgatgat tttcatcttg aacacctttg
     5521 tttttagcaa ttcgcaaaaa acgaaaagta ggagaattga taaatgaaaa aaagattttt
     5581 agcacttgcc atcgtactag gcactggctt attgtcaggc tgtacgaatg ccggtgaaaa
     5641 aacagccgtt agttataaag gcggtacaat cagcgaacaa gaagtaatgg atagcttgaa
     5701 aaaaatgcaa ggagcagatt ccgctgtgca acagttgatc gtttatcaag tatttgaaga
     5761 taaatatgga gatgacgtat caaccaaaga aatcgattct caatatgatc aaacaaagaa
     5821 acaattgggt gattcatttg attcacaatt gaaatctgca ggttacacag aacaaacatt
     5881 caaagacagt atcaaacaaa gtctagcctt tcaagaaggt ctaaagaaac atatcaaatt
     5941 gaccgatgaa gatttaaaaa cggcttggga atctttccat ccagaagtgg aagctcaaat
     6001 tatccaagta gcaagtgaag atgatgcaaa agatgtgaag aaagctgctg ataagggaga
     6061 cgatttctct aaattagcca aagacaagtc aactgatact acgacaaaag aagatggcgg
     6121 aaaagtgaaa tttgattcca ctactacaac ggttccggca gaagtcaaag aagcagcctt
     6181 taagttgaaa gatggtcaag tttctgatgt gatcacttct accaatgctt caacttatac
     6241 tacggaatat tatgtggtta aaatggtgaa aaaccaaaac aaaggcaacg acatggataa
     6301 atacaaaaaa gaattgaaag aaattgcaac agacacaaaa ttaagtgaca gtaccttcca
     6361 gaataaagta attggcgaag tattgaaaga cgctaatgta aaaatcaaag acaaagactt
     6421 tgaaaatgtt ttgagtacat tcacttcaga ttctagtaca gcaagtagta aataagcggt
     6481 ataaataaaa aatatgaact atttaaatca ataaaactgt tcatctaaat ctttttatta
     6541 gaactaaatc aacagtagtt taattactac ttgatttagt tctttttatt ttaaaaattt
     6601 tttttactta gaatccagcg accctagata tttttctgta taatcttaaa taaagaaaac
     6661 aatatcaaaa aatgcacgta gaagtagata aatggagtgg ttttgcaaat ttcttttatt
     6721 tctagaagtt attcacgtat acggatttgt ttttctcaca aataaaaagg caggaagtac
     6781 aatggatcat ttgtatataa aatatattca tcaattttcc gttttggtgt taggcacact
     6841 aattatagcg gggattgtcg catttattat tcaaggccgt gacatattaa cagtacatca
     6901 agagtttttc agttatgcat ttggtattcc tctgatcgct ataaggtctg ataacaaaaa
     6961 gtggaagaaa cgctattaca ttctcttgct atttttgtgt ttttatctgc cacctaagat
     7021 ttttacttta ttaggtatac aatgcctatc attttctttt atttcctata tcattgtttt
     7081 aattttcctc agtttcatag tatggatgtt ttatcttgtc aaaaaataaa tgaatgtttc
     7141 atgaacagta cttcatcaca gcctaatttt tttctttatg ctatcgttat attaaagaaa
     7201 tcaatcagaa gggaaagaaa gtgatgaaaa aagttttatt agtggtaaac cccagttcag
     7261 gcggtgaaca agcaaaggaa tttgagcaat tagcgatagc aaaattggaa tcggtgtttg
     7321 atgaagtggt cgtcttgcat acaaaaaaag caggggatgc aaaaaacttt actcgcgaag
     7381 cggctacgga agggtatcat agtgtgtttg tcatgggcgg agatggaaca gtcaacgaag
     7441 gaatcagtgg aatcgctgaa caagaacatc ggccaaattt cggatttttc ccattgggta
     7501 cggtgaatga cttggctcga gcattaggga taccgctaga acctgaggaa gcgatcaatc
     7561 atttttcgat tgaatcagtc aagccattgg atattggaaa aataaatgac gactatttta
     7621 tgaatgtcgt cgcaattggc tctatccctg aatcaatcaa tgatgtagat gcggagaaga
     7681 agacgaaatt tggaaaacta gcttatttca tgtcaggaat caaacagtta gcttccacac
     7741 aatcctactc gtttcacgta gaagttgatg gaaagaagga agaaattgag agcagtacct
     7801 tgctgatagg actaactaac tcagtcggcg gttttgaaac attacttcca aatgcaaaag
     7861 tggatgatgg taagctccat ttggtttatt taaaagattc ttctttgatc gatacgctta
     7921 aaacattgcc agacctatta aaaggagtag atgaatctac gaaaaatcta gcctacctta
     7981 cttgtgagca attagttgtg tcattagctg atgaggaaga attgacgacg aatgttgatg
     8041 gagatgaagg ggataaatta cctgtgacaa tcaaagtatt gccctcacat ttacaggttt
     8101 attgttaaaa tggaggttgt gaccgaggag ttggcgggag aacaccgctt gttcgggata
     8161 agggactgtg acaagacttt tgtcacagcc ttttttttgt acaaaaaaga aatttacacc
     8221 taatagaaaa atttttccat aaaaatacat attttaaata tgaaagtaca attggaaata
     8281 gattaaaacg ataaaataaa attgtcaaaa cgtcagagag gatggtggag aatgaatcaa
     8341 tgtgtaaaaa agctgattgc gtcgattttg tttgtcttcc ctctcttgat ggtgacatcc
     8401 gtttttgccg atgtagagaa aacaaacgaa tcaacgcaat cagagaatca aagttttgac
     8461 aagactactt tttcagaaga atcgattact tctgaaacta cagaatctca aagtgaggct
     8521 tctactaata aaaggcaaga agaaaataag acaagtgaga cctcagaagt agctgaacaa
     8581 acgtctgata gcagtgaagc ggcactggca agagctgttt cagcggatgg gtacagcgaa
     8641 gctgccacaa cagaagaact ggcgcagctt ctcgcagacg aatcagtaac taagatccga
     8701 ctgattcagc ctttgacttt agacagggaa ttagagatca aacgagatat cgttattgat
     8761 tttggtggat tcgctcacaa tttcggcaca caccatattt atatcaatga gatacctcag
     8821 tcgatcgagt tccagaattt taaaggaaca gctgcacatc caggtggatt gacaccaaat
     8881 atcgatggaa atgccattat cattgcttat atgaccgatt tttggggaag cagatatcat
     8941 tttacgggag aaataaaatt taccgggaca cttgatttgt atgatgggtc caaattagga
     9001 ctgatttacg ctcctcgtgc aactgtcaca ttagatggtg tttccggtgt gttggatgtc
     9061 caaccagtga aggaaggact gaatgccgct ccgggtaaag cctatttttg tagaagctat
     9121 caattgaatg tgatcaatgg tagtcaactt tatggaccgt atctagggaa attctacggt
     9181 tttctagatt caggagatga gactggtagt ggaaaaacag cgccaggaat ccatatctta
     9241 agcggttcaa aggtctctct tgattatgat cgggcagatg gcgttagcga tgaaggagag
     9301 gcagtcgata cgctacctgg taatgtcatg tttgaagtca gtggttcggg aagcgaattt
     9361 tcggtaaata caaagatcaa tattaccaat gacaacaatc gcggaatcat ccaaatgaga
     9421 ggttcaggat cgaaagtcac tgtatcgaat ggtgggaaaa tcaccatcaa tacagcaact
     9481 acgggcggat ttcgcttgca aggagattct agtcaaatcc atgtatcaag tggtggacaa
     9541 atctcggtca aaatggctgg agatgacgat caacccggga acaatgggat gcgttttgta
     9601 ggaaaaggcc tttctttgat cgtagaagga gccggatcag ttatcgacat cgataagcag
     9661 agtgggcgtt ccagcgcagt tcgttttgag aatggtacgc aaaagctgac agtaaccaat
     9721 ggaggctcat tgagggtaag aaatacaggt gatggcaaat cttacgtgaa cagactcaat
     9781 caaggaaatc aagcgatcca attttatgac gcttctggtt ttctagaaag tccaggttca
     9841 gctgatttta cagtcactgg agaaaaatca aatatcgata tacgcgcaga ttccggagca
     9901 actgtagata caactggaga tattgattta aattttacgg caggtgaaaa aacttattta
     9961 gtcatggtcg ggaaaacagg ggaagataag accggcatca tcagtgggga tgtgttgaat
    10021 gtggacttag aatctcctac ctattttgat ttccaaaaca aacggattgg aagcgagacc
    10081 aatggcatag gcggctgggt attccaagga aatggagatt cttctttaaa gatcgcccat
    10141 tcagaaatcg ctttatggcg aaaaacaggc gcaacattcg ataactttga aaaagacccg
    10201 gattattttt ctgatatgac agacttatcg atcaatggga ccgacttagg caatttcgta
    10261 tcaagcccta gtgcaggttt aacaaatgaa tttgctcaga caggtgccgg aatgaaaaat
    10321 tataatcgaa tcagtgccaa taatcagctc ccgatgattg aatccttaag agtgccaacg
    10381 aatgcagata aaaaaatcta tgggcatgtc gttgttcccg aaggtgtcga ggcaaagcca
    10441 agagatgctt ggacaggcga agtagaagtg actttacgta ttacttacgc agatacgacg
    10501 aaatcatctg tagaaattac tgctctcacg caaggacaaa cagatgaaaa tgaaggggca
    10561 ggatataacc catggggaga aggcgaacaa ggcggcttgt tcgaagttca agtacccaat
    10621 gatgagagat taaaaacgga ggatcaagtg caagttgttg ctgctaaaaa agtaagaggt
    10681 ggccaagaag tgacagaact ggactctgcc aaaacgacag tcgatgtgat tcctcctgaa
    10741 cctgctaatt taagtaccac attgatcaat gcagcttctc gcacgatcac aggtacaggt
    10801 gaagcaggag ccttggtttc attgaaaaaa aatcaagctg ttttgtcaga aacaactatt
    10861 gatgatacag gggaatttgt tctttctatc ccagaaaatc aactttttgc aggagatgta
    10921 ttagaaattg tattaaacga ccaagcagga gaagctactg ctaagggtgt gcagaacaag
    10981 ccaagtacca acgatgaaat tgggaatcac aatccaagtg atacgccagt tgtttatcat
    11041 gatgccccgc cttttgcacc tgctacgaag atcgtagtag aaggtggtct ctcatttctt
    11101 gccccttcca aactcgattt tggtcagatc aaagcaactg gtttgacaca agaagcattt
    11161 ggccaaatgg atatacagga aaaattatgg atttatgatc agcgagaaac gaaaaatccg
    11221 tggatcttaa cgataaaatt atctcagcct ttcacaaatg atgcaggatt ttctatgcag
    11281 gatgtcctat atttcaagaa aggagaggag tatcaattag tgaatgaaga agaactggaa
    11341 attgtcagag gacaaaattc aacgaatgtg caagcagatt attcagaata tttaaataac
    11401 ggtcgggcgt ttaaacttgt cttaaccaaa gaaaatcaga tagctggtaa ttactcagca
    11461 gagataacat ggacattagc agatgcgccg acagaataaa gatcaaggag gatgattgat
    11521 gaatcgaata tggaataaaa gtcatcgaaa caactgctct ttacttatga tccttctcct
    11581 tggattcatc ctttggcaga cacctgtcca tgcctccgaa ttcaattttg cggtagattt
    11641 tgaattaccg gctaatcaga ttgataagaa caaaacatat ataaatgtaa ggatggagcc
    11701 tgggaaaaca caggaaatcc cctacacggt ctttaaccga acagataaat cagtaacagt
    11761 tgagacattg atccactccg ctacgacgaa ttcaaacggt gtgatcgaat atggagaatc
    11821 aaaaagtaaa tttgatgaaa cattaagtta tgatatgaaa aacttagcaa aaacagtggg
    11881 agaaataact gttcctcctc aaagtgcggt cactgagaca ctttcactga aagcgccaga
    11941 aaaagagctg gctggtgtgg tcgcaggagg gatcacgttt caagaaaaaa aagagaacgt
    12001 ttctaacaaa agtacagttg gtgtcgtcaa tcagttttct caagtgaaag caatcttgat
    12061 ttgaaatagc gaaaaagagt cttccccaga tttgaaatta aaaaaagctt atgcggatca
    12121 aagcgatgga agaaatgtga ttaaggcgaa tcttcaaaat ccaaatgctt cttatatgtt
    12181 cggcttgact acatcggttg aaatctacaa aaaagaaaca aaagagcctt acctttcaat
    12241 aaaaaaagaa aaaatagatg tagcacccaa tacaaatttt gatttacttg tcccattaga
    12301 aggaaaaaaa ttagctccgg gacattatca agcggttgtg aagggtgctt ggaaagaaaa
    12361 acgctggaat ctccataccg attttgaaat aactgatgat gcagcggacc aattgaatga
    12421 aaaggatatt gatttaaaaa atcagccaga aacatttgac tggcgatggc tgatacttgt
    12481 tggagtcatt cttattggct tatttgctgg attatttatt ttaaaagaaa gaaggaaaaa
    12541 gaaatgaaaa aaacaattgc attaagtacc gttctagctg gaagtctatt attatgtaca
    12601 caaagtgttt ttgctgagaa taagagctca gccactacag aaggaacgat ttctgtgacc
    12661 aaaccagatg ctgcaaatcc tgaagacgtt gttccagttc ctcctgttga tccaaatgat
    12721 cctgatactc cttttacacc tgacgaacca aatcagggaa catccggatt actgacgatt
    12781 gaccaagctc cagatcttga ttttggaaca atcaagttag gtacagcaaa aacagtatat
    12841 gctgcattac aaagcggaac ggacagtact ggagcacaaa cacaagtaca gaatttcgta
    12901 caagtaacgg ataaaagcgg aaattatgcc ggttggaaat tatcagtcaa acgaacagaa
    12961 tttattggta caacatctga tacgaataaa ctaactggtt caaaggtgaa attcaaaaac
    13021 gccgtgattc gaaatagcgc aactaatagc agtggaatgc catctaccgt ttctggaact
    13081 ggcgctgaag gaatcgaaat cccagcagat caggcagtag atctagtaac tgcaggagca
    13141 aatgaaggga ttggcacatg gatttattca ttaggagaga atgtggaaca agggagacaa
    13201 tcagtggaat tatttgttcc tgtatccaat tatgctgtag acaactacgt ttcaacattc
    13261 acatggtcgt taacagatgc tcctacagaa gaggaagcgt aaaatcatat caacgatttc
    13321 agggagtgga gccgattcaa ggcccactcc ctgaaatctg ctatgcttac gtctcagctc
    13381 ttattcatca actcgaagca gttttttgga agtcatggaa acgttccttt gtcttttcaa
    13441 agatctttcg accaacgagt aaggagtcac cgttgaaaaa ttctaacaca taatgagcag
    13501 aatcaatttt ctgcaattgc gataagttga tacagtaaga ttttaaaagt aaaaagtaag
    13561 catacttttc taactctgtc ttcaattgtt ttaaagaagt attcactaat ttatcttctg
    13621 tagttgtttt gaccataact tggtttttca aacctttgat actttcaata tataaaattt
    13681 cccgaggata caggacgaga cttccacctt tttgatcaat aaagatttta tcgacattta
    13741 cgatctgctg ttctttccaa ttgatctgtt ctagcacttg acgaatttgt tcagataaat
    13801 tcctgtcttt tctgatatat ccaatcggaa ggattttccg attgatgatc tctactgcaa
    13861 aattcgtata agcggtgaaa aaaacaatat tactatgtgc atttttttta cgtatttgtt
    13921 cggcgacatc aatacctgtg agtgattgct tcaagtcgat atcgataaag aaaacatcgt
    13981 gattatcaat agtgcccaac tggatcaact gattgatttg agagatagaa gtgagctgca
    14041 cgatattctc gatatgatga ttgaccaatt ctctgatgat ccattcgctg tgaaactggt
    14101 tatcttctat gataaaatac tttttcatat gcaggctcct attaaaaaaa tatgtgtaca
    14161 ttttgaaaca aaacaatgga ggaataagat ggtcgcaaca gtgacacttt atttgacctt
    14221 tactttgatt ttagatttag gtatctattt attcttttcg aaacaactgc tagtaaaaaa
    14281 cagctttggt tgtatagctt tgatggtggt gacactttta cttcctatcc ctaaagtgag
    14341 ctttccttac ctagttggct tattattgat gtttcttttt tattttacta cgaaaaacct
    14401 gtttatcagc tgtttgatca gtaatctttt ttttgctatc actggactca gcttttttct
    14461 ggtttttgat ctgatggatt atttaggaaa gagacatctt ggaacagcaa gtttttttgc
    14521 actacttatc gcatttattt tctatctatt agtaaaaagt atagacaaac agacgagatt
    14581 tctcaacttg ctttttactt atccaactga acactatttt ggaggtattt gtattttatt
    14641 aagctggggc gtgatttact tggtattggg aagtgttcac ttccgctcat ctaattatct
    14701 gcttctatct cttttgtcga tagtcatcag ttttttttct ttgttttttt ccattatgtt
    14761 gatcagtttc caagtaaaag aaagacaaca tcaggaatcg cttcaactgt atcaaataaa
    14821 tgaagaatat taccatcatc tagaggaatt caaacatgat tataaaagtc tactgttttc
    14881 attaaaaatt actcttcagg aaaataaaaa agaaacgttg gaatttttag aaatattaga
    14941 agatcattct gcagctattt ttgaaaaacc gcaaatacaa cagctaacaa atattgtatc
    15001 cccagctgtc aggggtgtct ttttaaaatt tctagaaaaa gcagaatcgg agaaaatccc
    15061 gatccaagta gttattcctg tcgaagtgaa aacaatcccg attgatctgg ttgtttttat
    15121 tcgctgttta tctattttta tttctaacgc attcgatcat cgtgtagccg atcagagtcc
    15181 gattacgatc aatctgctgg aagaaaaaga gggatcacgt tttagtatag cgaacaaggc
    15241 aaatccaacg aatttgacgc tatcggagat ggtcaaaaga ggaaaaacct ctaaaaaaaa
    15301 tggcgggtta ggtctataca gtgcgaaaaa aatgttggat gtttacgaaa atgcagaact
    15361 aaaaatcgaa ttttcaaaac agaatcacta tttttttgta gaaatgtatc tggcatctag
    15421 aaagagcaaa tcagcctact gacttactgt caaaggataa gtttgaggaa agtctacacg
    15481 gagatagaga caacaacaca gcatattcaa aagaaacaga agcgtgatga ccgttgtcac
    15541 ccttctgttt cttttgctat gtagttaaac aaagtagttg acacttccgc tacttagtat
    15601 tagtacgaaa gtatacttca tgatactaag tagataagaa gtgaaaagcg gaacccacga
    15661 tcgtcaaaat gctaactgct ttgcttacac cagataaagg gaagagcaat atcgcttatt
    15721 ttaggcgtgg agaagcaagc aaatccctac gatcacaaaa aaaatgctga cccagcagtt
    15781 aagagagtta gaacaagatg aaagtatcca tcgaaaagcc tataaccaag tcccgcctaa
    15841 agtagagtac tcgctcaccg aagaaggaaa atcattaaga gagattctag tagcgttgtc
    15901 tgtttgggaa gaagaacgta tcgaaaaaca aaaagcctca agggaagaag acacgctact
    15961 gcatacacat ggtggctacc taaattattg attttctatg acatttattt taaataaaag
    16021 aaaacaggag tttttttaaa agcatcatct attttttatg taaaaaaatg gtatgattaa
    16081 tcaagtaaga agtgattgga agaaggcaag caaatgaata gtatggaaaa agtcaaccag
    16141 tttagagatg aacgtaattg gcgacaattt cataacgaaa aagatttggc tatctctatt
    16201 tctttggaag catctgaatt gttagaactt tttcaatgga aaacgccgga agaagtaaaa
    16261 gaaacacaat tagaacgaat caaagaagaa ctggcagatg tattgatcta ttcatacatg
    16321 atggcagaca accttcacct ggatctagat gagatcattg aagaaaaatt gatcaaaaat
    16381 aatttgaaat atcctgtaga aaaaagcgca ggtgtacgaa aaaaatacac agagttgtag
    16441 aaatgtgtca ttcggctgtt ggaaagagca aaagaaaaac agtaaaatga gtctggaggg
    16501 agagaaaatg aaaaaaaaag gaaaagcaaa aagatggcta gtcaatatcc tcttgttcct
    16561 cttgttatta gtaggactag ctttgatttt caatgaacag atcaaggatt attttgtcag
    16621 agagacagga gataaatatg ctatagcgaa tgtaacaaaa gaagatctga aaaagaataa
    16681 cgataaagat gtcagttttg actttgatgc ggtagaacca atgacgacag aaggggtcat
    16741 gaggtcgcaa atgagaggaa cagatctacc agtgattgct agtatcgcag tcccttcagt
    16801 ttcaatcaat ttaccggttt tcaaaggctt ggataatacg tcactacttt acggagcagg
    16861 aacattatct ccagatcaag aaatgggaaa aggaaattat gccttagcaa gccatcgtgc
    16921 aacgaaccct gaattgctgt ttacaccgct agaaaattta gagatgggtg ccaagatcta
    16981 tttgacagat ttagaaaatg tgtatacata caaaacattt tttaaagaaa aagttgctcc
    17041 tacagatacc caattactga atgaagtcga gggaaaagaa attgtcacac taattacttg
    17101 tggcgacatg gacgccgtca cgcgactagt tgtacaagga gaactggaaa gtgttacatc
    17161 gataaaagat gcgacagatg atatgagatc tgctttcaat ctagagacaa aaacatttta
    17221 ggatactggg acataagtcg ttacagatta aaaacagcca aaggatatga atcgataagg
    17281 aaaaaccaat ttgctgaaaa aaagagaaac gcaaagaggt gcatctctga tttggttgtt
    17341 ttctagatag acttgtgttc cagttttttt attttttata tgaaaaggct tgcataaata
    17401 aaaattcgat ggtatgatga ttttgaataa gaaatggatt gttactgtac ggcaggcaaa
    17461 accagaattc agctaagaaa cagtaggcga attctaggtt tttttatttt tcttgagagg
    17521 gcgaagcaaa atgaaaattg ataaaatttt gaataacaat gtcgtcattt cgaaaaatgg
    17581 attcggggaa gaagtcgttt gtatgggaaa aggactagct tttcaaaaaa agattgggga
    17641 cgagatttcg ccagaagcgg tccaaaaaga atttgtttta agagattctt ttgctaaaaa
    17701 ccaattccag cagctgatgg ctgatattcc ggcagaggag atcgaatggg tcaaacaagt
    17761 agtcgaactg gctgaagata aactaaaagt tgaattttcg cctaatattt atctgacatt
    17821 gaccgatcat cttcattatg cgatcacacg agcaaaagaa aatatccaac tgcctaatcc
    17881 attattattt gaaacaaaaa aattttatcc gaaagaatat caagcagcga aagaggctct
    17941 tttgttcgtg aaacaaaaga cgggtgtgga actttctgag gatgaagccg gttttattgc
    18001 ctttcatttt gtgaatagcc aacaaggaaa cgaagacatg caagtgacga tgaccgctac
    18061 cacaatggta agagatatac tgagtatcat cagtaaattc ttcggcatcg tgttcgatga
    18121 agaatctttg aattatcagc gtatcattac tcatctgcag ttttttgccc agcgttatct
    18181 caaaggggaa ggatctgacg agcaagatga atttctctac gcattagttc aaggaaagta
    18241 tccaaaagcg tttcattgtg tcgaacggat caatgagtat cttcgacaaa cgcaaaaaca
    18301 ggaaatgggg atcgcagagc agatttattt aacgattcat attcaaagag tagtaagtga
    18361 gaaaaaaagc atccaatcat aaggattgtt actgtatggc aggcaaaacc tggattgaaa
    18421 aaataggttc acgaatgttt gtgactgttt ttttcaatct gggtttttat tttttaaata
    18481 aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt ggtcgtcgtg tatgggaagc
    18541 ggtaggagga caaaagaatg tcaaaagttt agtacattgt gctacgcggt tgcgattcag
    18601 attaaaagat gaatctttag cagatacgca aaagttaaaa gaagacccgg acgtgatcca
    18661 agtcgtacaa agtggcggtc agtatcaagt agtgattggc agtaatgtag cagatgttta
    18721 tcaatcgatc gtggatgagg aaggtcttgc agtttcggag gatggaaaag aagagtccaa
    18781 aaatattttg aatcgtctga tcgatattat ctctagtata tttacacctt ttctaggggc
    18841 gatggcagcc gccggggtat tgaaaggatt cttatcattg gcaacggtga tgggctggct
    18901 gacagctgat agcggtactt atcaaatatt gtttgctgca gcagatggtg tctttacctt
    18961 cttacctgtc atgcttgctt ttacagcagc taaaaagttc aaagccaacc agtttttagc
    19021 agtagcaatt gctatggcac ttgtgtatcc agctatcaca gcagttgctg gtgcgggaga
    19081 agctatcagt ttctttggga tcccagtgat tctttcacca tctggttata cttcttctgt
    19141 cataccaatc attctagccg tatgggttca aagtaaatta gagccattcg taaaaaaagt
    19201 gattccgcaa tttctgcaaa tgattttagt tccgcttgtc gttttagtag tgatggttcc
    19261 attaacattt cttgctttag gaccaatcgg gacggttgcc ggtaatgctt taggcggtct
    19321 cttcaactcg atttatggat ttagtccaat cgtagcaggt ctaatcatgg gaagtttatg
    19381 gcaagtattc gtgatgttcg gcatgcattg gggattcgta ccgatcatgt ttttgaatat
    19441 cgaacaatat gggtttgatg tattgatgcc aatgcttctt ccagccatct tggcacaagg
    19501 tggggctgct ttagctgtag cattgcgaac aaaagatacg aaacttcgag cattagggat
    19561 ttcttctaca gtgacttcgt tatttggaat cactgaacca actgtgtatg gtgtgacatt
    19621 acctttgaag aaaccattca ttgccgcttg tatttctgga ggtattggtg gagctattat
    19681 cggattttcc ggagtaaaag ctttctccag cagtttggtc agcttattga cgattccgac
    19741 tttcattaat acggtggatg gtgtagagtc aaatgtgaca gtagcagtaa tagctacagg
    19801 aattgctttt gttcttgcat tcgtaggtac attgatttta ggatttgacg aacaaacaca
    19861 agacaatcaa ttagaaaaca aacatgcgaa tgcaggagaa ccgattactt cagcacggca
    19921 taccttaaaa agtcctttaa cgggtaaagt actgccattg agtgaagtac cagaccaagt
    19981 tttttcttct ggcgtgatgg gaaaaggtat cgctattgat ccagaagtag gagaactagt
    20041 tgcacctgca gacggagaga tcacgactat ttttccaact ggacatgctg tagggatcac
    20101 aacgactgac ggagcggaga ttttgattca tatcgggatg gatacagtag aattaaatgg
    20161 gaatggcttt gagatcttag tcaaacaagg ggatctcgtc aaagcaggag atttattgat
    20221 tcgttttgac atcgaagcaa ttagagcagc tggctatagc gtaatcactc cagttgtcat
    20281 tacgaatact gatgcatttg ccgatatact tgaattagac caaaaagaga ttattgcaaa
    20341 cgaagatgtt ctggcaattg taaaataagg aggaaatcag gatgcaatca cgtttttcag
    20401 aaaaattttt atggggtgga gcaactgctg ccaaatcaat tagaaggggc ttatgatcga
    20461 gacggaaaag gactatccgt tgcagatgct atgcctggag gtaagcagcg ctttgcgatt
    20521 atcggcagtg aagaatttga ctggacgatt gatcaagaaa aatatattta cccgaatcat
    20581 cgaggaatcg atcattacga tcgtttcaaa gaagatcttg ccttgtttgc ggaaatggga
    20641 ttcaaatgtt atcgtttttc gattgcctgg acacgaattt ttccaaatgg agacgaaggt
    20701 actcctaacg aagcgggatt agaattttat gatcagctga ttgatgagtg cttaaaatat
    20761 gatattgaac ctgtgatcac gatttctcat tacgaaatgc ctcttcattt ggcaaaagaa
    20821 tacggtggct ggaaaaatcg aaaactgatc gacttctatg aacgatttgc tcagacagta
    20881 ttagagagat acagcagtaa agtcaaatat tggatgactt tcaatgaaat caattcggcc
    20941 tttcactttc cagctttaag tcaaggatta gtaaaaagca atggagcagg agagtaccaa
    21001 aatattttcc aagcatggca taatcaattc gttgcaagta gcaaagccgt aaaaatcggc
    21061 cacgaattgc gttcggatat ccaaatcggc tgcatgatta tctatgcaac aacttacagc
    21121 atcgatgcca atccagtcaa tcaggcagca acaatgatcc aaaaccaaga attcaatttt
    21181 ttctgcacgg atgtccaagt acgtggagaa taccctgcat acacagcgcg tacccataaa
    21241 aaatatggag tcgatccgga aaaattagag caaacggaaa aagacttcca gttgctaaaa
    21301 gaatatccag tggactacat cggatttagt tattatatgt ctacggcaat caatgagact
    21361 gatccacaag cagcaacttc agaagggaac ttgctgggag gagtaaaaaa tccgtttctt
    21421 gaggcaagtg aatggggctg gcagatcgat ccagaagggc tgagaatcgc attgaatgaa
    21481 ttatacaatc gttaccagaa accattattc attgttgaaa acggtcttgg agccattgat
    21541 catatggaag aggatggcac gattattgat gattatcgga tcgattattt acgtcgtcat
    21601 atcgaagcga tggctaatgc agtagcagac ggtgtagatt tgatgggcta tacgccttgg
    21661 ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga gtaaacgtta cggctttatc
    21721 tatgtggatc tagacgatga aggaaatggg acactgaatc gatcaaagaa aaaatctttt
    21781 gattggtata aagaagtaat tgcctcaaat ggcgtaaact tgtaattaaa gcagctgaat
    21841 gaaaaagaac acacttctca catgtgattt gtgttctttt tttatttttt atgtgctatt
    21901 cttttagtaa tagtaaagca aagggtgacg ttatgaaaag gacacgtgta ctatacttgg
    21961 aagtcgcaga taaaattaaa gaagatattt tctcaggaaa atatccagtc ggttcgatgt
    22021 tgccgacaga aagtgaatta gaagaactgt ttcaagtcag caaaatcact gtccgaaagg
    22081 caatcgaact gctggcatca gatgaatatg tcgcaaagaa aagtggtcgt gggacgacgg
    22141 ttctaagcaa tcgtccttac aatcgattat ccaaagcggc aagctttacg caaatcttgg
    22201 agaactcccg atatgaagtc cggaaagaaa cgctggattt gcaattggtc catataaaga
    22261 aggaagatcc tttgttttcc tttttcggtg agcaagctgt ttgttttaag cgcttatatt
    22321 atttgaacaa tcagccttat atttattttg ttcattatct tccacaagaa atgatgcaat
    22381 tcagcagaga agaatttggc aaagaatctt tgtatcgttt gctgacgaaa aaaggatata
    22441 ctattgactc attttctgat gatttctcgg cagtattttt aacagaagaa gaacaaggaa
    22501 ttctgaaaac agacgcacag atcggcatca aacggatacg gaaatcgtct acgcaaattg
    22561 gggaaatgat tgaatattcg gaggcggtct accatacgga gctgcatcct taccatattg
    22621 attatgaaac ataaataaaa aaagaggttg tgaaaaagct gccctgcatc aagtaataag
    22681 aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttaggcttat taccgcagat
    22741 acacggctgt ttctggatta ccatttattc tggataaggg ggtgtggcac gattttcaga
    22801 aggaatctat gaaccaatca ctgtattgat tttagcttcg atggtaaccc cattcggctt
    22861 gacgatcgct tatttcttag ggaaaatcat tcgtaaaaat atactcaatc gtcaagaaat
    22921 cgatacattg aagaccgctt ttcctatggg gatctgtcag atcacagagg gatgtttccc
    22981 tatcgtattg aacgacttgg cacgcaatgt catagcaacg ggagtcggtg ggacagtagg
    23041 tggcggatta agcatgtttt ggggcgcaga tagccgtatt ccggcatcgg gaatgtttgc
    23101 agtcgctacg atgaccagac catgggcatt cattggtgca ttgttagctg gttcttttgt
    23161 cacaggaata accattttat tattgaaaaa accagtagat ccgaatgcag aaatcatcca
    23221 agaagaaaaa gaggaagaag acatatcttg ggatgatttg acaattagtt aggagaggca
    23281 gaatggatta caagaaaaaa gtacaataga tgtaccttga gacaggaatg gtatgaaaga
    23341 atcagagtta gaaagaatcg aatatacaga ttttggttta ggagcgatcg aaactgtcca
    23401 gagcatcggc accctccttg aaatgacgat gaaggaaaaa tgagaacctt tcgttgtcga
    23461 aagggtctgg tataccttat gtcgaaggaa aggaaacaaa aaaactttcc tgtactattc
    23521 caaaaaggaa agaaagttat tatacagtaa aaaaagaaat tattcttcat gctagagatc
    23581 agtacacgtt cgagcccaat atcaaacact agttccaggc aggcgcaaca ggtgcggtta
    23641 tcactgaatt ttcttccagc agtgacgatt caagtgatat ttttacaaat ccagctattt
    23701 atcgttaaca aaaagatttt taggagaagc tgatgaacaa aaatgatcaa attttgatcg
    23761 tttagttcgt cggctttttt atttagagaa acatcattta tcagtgaaac ataacacgag
    23821 aacaaaatga ttttttcgtt aataaaagaa acaagtattt gttgtttaaa ataatacacg
    23881 ttattggcat ataggacgaa atcccagtat cttctataag taagtcattt catatcgatg
    23941 aaaacacgtt atttattttt gttttatcta taaaatggct gaaaaatgat ttttttctaa
    24001 atactatata tagtggcgtc tatcctatat accactaaaa aactacaaaa tctagtcttt
    24061 tattttaaaa aaacacgtgt agaatgaaga tagacttttg agttagggag tgttttttta
    24121 tgaaacttac tgttttttgc ggttcccgtt ttggtaataa agaaagttac aaatttattg
    24181 cgcaaacgtt aggaaaatac atggcacaag aagaaatcga actggtttac ggtggttcat
    24241 actccgggat tatgggagtg attagtaaaa cagtactgga aaataacgga aaagtcacag
    24301 gtatctatcc gaatgggctt tttgaaactg aattgcctgg aaaagatgtt actacattta
    24361 ttcctacaga aacgatcgat gaaagaaagg tattattatt tgaaaaggga gatgctgtcc
    24421 tcgttttccc tggaggtcta ggtacattgg aggaattctc acaacttctt tcttggatag
    24481 caatcggtct gacgcccgac aagccaatcg gaatattgaa tattggcggt tattacagcg
    24541 gcttgcaaca attattagaa acttttgcaa aagaggattt tatggataaa aaatggttgg
    24601 atcaagtaat cttttccaac aatccattgg aactggtcaa tgaattacga gcagtatcta
    24661 ttaaaaatca gttattattg aaggaggcaa aataatgagt caaatatatt tagcaggacc
    24721 atttttttca gaggagcaaa tcgatcgagt aagtcgtatc gaaaaagcgt tggaggaaaa
    24781 caaaacagtc acaagtttct actctccacg tcatcaccaa gaaagcaatt atgaactatt
    24841 cagcgctgga tgggcgcagg aagtatacga aaaagatatg gaagaactga caaatgcaga
    24901 atttgttgtt gctatccttg actttgagca tcaaacaatc gatccaggaa cggcttacga
    24961 attaggtgtg gcaactatgt tgaaaaaacc gatgatcatt gtacaagaag agacggtacc
    25021 tacgaacttg atgattacac aaagtctcca tacgtattta aaatcagacc aagctgtacg
    25081 tgaatatgat tttgaaacat tgcctgttga aacttacgta ggcgagtact tatagaagag
    25141 gaagggttgt atgtcagtga ttttgatttc aggaacgatt ggtgcaggta agagcagttt
    25201 gacggatatg cttgcaaaag agattgattc taaaccattt tatgaaaatg tcgaagataa
    25261 cgaagtattg ccgctctttt attcaaatcc tgagcagtac gcttttttat tgcaaatctt
    25321 ctttttgaac aagcgatttt tagcaatgaa aaatgcttta gtgaacgatg acaatgtgct
    25381 agaccgatcc atctatgaag atagcctgtt gttccatcta aacgctgatt taggtcgggt
    25441 aacggatatc gaagtacagc agtacgatag cttattagat acgatgctaa acgagttaga
    25501 tgatgttgct ccaaaaaaac ggcctgattt gatggtccat atcaaagttt ctttggacac
    25561 gatgctagaa cgtatcaaaa aacgtgggcg tgactatgaa cagctagaaa gcgatgagac
    25621 gttatatacc tattatgaaa cgttgaacac ccgctataat caatggtatg aagattttga
    25681 tgtatgtcct aaaatacaag tggatgggga taaattcgat tttgtagaaa atccagaaga
    25741 tgcaaaaaag gtgatccgtc agatccaaga aaaattagct gaattggaag gaaagcctgc
    25801 tggaaaatat tttacggata accagcatgc aaaaggtcaa gaattaggct aaaaa
//
LOCUS       DAFCNS010000038        25153 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFCNS010000038 DAFCNS010000000
VERSION     DAFCNS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.038"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            226..894
                     /locus_tag="LNE23_001535"
     CDS             226..894
                     /locus_tag="LNE23_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBK6931392.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="LNE23_001536"
     CDS             complement(1094..1324)
                     /locus_tag="LNE23_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931393.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="LNE23_001537"
     CDS             complement(1451..2833)
                     /locus_tag="LNE23_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK6931394.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="LNE23_001538"
     CDS             complement(2989..4176)
                     /locus_tag="LNE23_001538"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBK6931395.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="LNE23_001539"
     CDS             complement(4183..5214)
                     /locus_tag="LNE23_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK6931396.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="LNE23_001540"
     CDS             complement(5260..6684)
                     /locus_tag="LNE23_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6931397.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="LNE23_001541"
     CDS             6859..7764
                     /locus_tag="LNE23_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK6931398.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="LNE23_001542"
     CDS             7855..8532
                     /locus_tag="LNE23_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK6931399.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="LNE23_001543"
     CDS             8529..8699
                     /locus_tag="LNE23_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931400.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="LNE23_001544"
     CDS             8813..9709
                     /locus_tag="LNE23_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6931401.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="LNE23_001545"
     CDS             complement(9764..11134)
                     /locus_tag="LNE23_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6931402.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="LNE23_001546"
     CDS             11372..12118
                     /locus_tag="LNE23_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK6931403.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="LNE23_001547"
     CDS             12115..12954
                     /locus_tag="LNE23_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK6931404.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="LNE23_001548"
     CDS             12955..13905
                     /locus_tag="LNE23_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBK6931405.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="LNE23_001549"
     CDS             14046..14798
                     /locus_tag="LNE23_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931406.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="LNE23_001550"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="LNE23_001550"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBK6931407.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            16352..16960
                     /locus_tag="LNE23_001551"
     CDS             16352..16960
                     /locus_tag="LNE23_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6931408.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            17128..18765
                     /locus_tag="LNE23_001552"
     CDS             17128..18765
                     /locus_tag="LNE23_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBK6931409.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(18837..19949)
                     /locus_tag="LNE23_001553"
     CDS             complement(18837..19949)
                     /locus_tag="LNE23_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBK6931410.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(20024..21397)
                     /locus_tag="LNE23_001554"
     CDS             complement(20024..21397)
                     /locus_tag="LNE23_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6931411.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            21618..22235
                     /locus_tag="LNE23_001555"
     CDS             21618..22235
                     /locus_tag="LNE23_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBK6931412.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            22333..22788
                     /gene="argR"
                     /locus_tag="LNE23_001556"
     CDS             22333..22788
                     /gene="argR"
                     /locus_tag="LNE23_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK6931413.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            22806..24308
                     /locus_tag="LNE23_001557"
     CDS             22806..24308
                     /locus_tag="LNE23_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBK6931414.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            24403..24609
                     /locus_tag="LNE23_001558"
     CDS             24403..24609
                     /locus_tag="LNE23_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931415.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            24695..25096
                     /locus_tag="LNE23_001559"
     CDS             24695..25096
                     /locus_tag="LNE23_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK6931416.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
BASE COUNT         8346 a         4682 c         5106 g         7019 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt tttatggatt attgcagtga atgttacaga atctgcgttt ttcattcaaa
    16141 aataaaaaaa catagaaaaa cgattacttt gcttatacaa caaggctttt atggttttgt
    16201 atcaaaaaaa tatttgttta aatgttacaa atattaaata attcgttatt ttgtcattaa
    16261 atgtcatgtt tcttttacct atgaaatatt tctaaatgat aaagttaata gcgtcgaaag
    16321 aataaaaaac ggagaaaaaa aggagtttaa tatgaattca tttaagaaaa ttgttttagg
    16381 tacaactttt gctgctggtg ctactgcaat gtttgtagga actacaaatg ctcacgctga
    16441 tgaagtttat acagtaaaat caggtgattc attatctaag atctcacaaa aatttgcagg
    16501 agacaactca atgatcgatg caattgctga aaaaaattcg atcgcaaaca tcaaccgcat
    16561 ttatgttggt gaacaattaa caatcccaac ttcaaatgat tcttcagcaa caacagaaaa
    16621 caaaacaaca gaaaatacag catctacaac tgaaacagca acgcaagaac atacatacgt
    16681 tgcacctgtt gaaacagttg aagtagctcc tgctgcccca gctgctgcaa cagcgccaac
    16741 ttcaagctct gcaaaagaat ggatcgcaca aaaagaatca ggcggttcat acacagctac
    16801 taacggacgt tacatcggcc gttatcaatt agatgcttca tacttaaacg gcgactattc
    16861 agctgccaat caagaacgtg tagcagaaca atatgtaaca tctcgttatg gttcatggga
    16921 tgctgctaaa acattctggt tagcaaacgg ctggtactaa gccgataaaa ggaaaatgat
    16981 gaaaccgaga aacttgttta ttcaagttct cggttttttt gtgtgaaaaa taactcgttt
    17041 gcttcccaca gcgcagcagt acaaataaaa actcgaataa tgtataataa agaaaacgtt
    17101 tttttgttta gggaatgagg aggagccatg agtacaaaag agagtctcga attaaacgaa
    17161 atcacaagtg gaaatccact gttatccagt attcgatcga ttgtagaaac gacattttac
    17221 ggtaataatg ttcatgaagt ttttgatagg aagacagctt atcaactagc aaaagggagt
    17281 ccaggaacga tcatcacaga tttaacaatc tcacatgctg aagaactaga tttaccagct
    17341 gatgttagga cactagtatt caatgatgga agcattgtgg gaagaacagc aagtgctcgg
    17401 cggatctttg aggacttaga caaggaacaa agcaaatacg agaaaatttt aagagaagct
    17461 gtgtatcaaa gtcgcaagcg ccagttttac catacaaaag taatcgttgg cctatctgaa
    17521 gaattttctg tgcaaagtca tttgctgatt ccagaaggat ttgagaacaa tttgtattct
    17581 tatttgctga attttcagcc agcgaattcg aagatcttag agacttatca aaagtctgtt
    17641 tcctttgatg agggagatct ttatatcttt tgtgatcccg aatggacaca tgaaacattt
    17701 ccaaatggac tcattttgtt tgatgctctc cataatacag cggctgtgct aggattacgt
    17761 tactttggag aattaaaaaa agcgactttg acgcttgctt gggcattggg aaatcgcaat
    17821 ggctttattg cctgccacgg tgggatgaaa caatatgcta gtaatgacca gacctatacg
    17881 atggctgtat ttgggttgtc cggttctgga aaatcaacga ttaccttagc aaaacatacg
    17941 gaaggtcact ctgtttcgat cttgcatgac gatgctttcg ttatttctcg aaaaaatggt
    18001 tttgctactg ccttggaacc ttcttacttt gataaaacac aagactatcc gtctgatgat
    18061 ccggccattt cttctttcct tacttgtcag aatgtgggcg tgacatgtac aagtaaagga
    18121 gaaaaagtcc tagtaacagg agatatacga aacggaaatg ggcggacagt caaatcacgt
    18181 tatgcgacac ccgaccgagt cgatcatctt agcgaaaaga tcgatgctgt tttttggatc
    18241 atgaaagatg attcgcttcc gccaattatc aagatcaatg acccagtttt agcagcgtct
    18301 tttggtgcaa cattagcaac aaaacgatca gacgcggaaa atatcactac aaaaagacag
    18361 ctagatgagc tagtcattga accatttgct aatcctttcc gtgtctatcc tcttagggaa
    18421 gactatcaag cttttcgaga gttgtttttg aaacagcaaa cagcctgcta tgtcctgaat
    18481 actggatatt tcgatgataa aaaaatcaaa cctgaaacga cattgtcaat cgttgaaaga
    18541 atcgtgcaac aaacggcaga attcaagtcg tttggtccat ttgaagacat ccactattta
    18601 cctataccag attttcctgt ttcttttgag gatacgtcct ataaaaatct ggtgaaacaa
    18661 cgacttgata ttcgtttacg attcattgaa gctcagatag gaaaagatct cttgccagat
    18721 gaagcaaaag aaagtttgga gaatttgatt cagaaattga actaaaataa cagctttttc
    18781 aagaagtaat ccatgcgcaa tatcgctcgg gatccttact tgaaaaagct gtttttctat
    18841 gatggatttt tcgtaatata ggtggagatg tccttttgat aatcttccgt aggccaatga
    18901 ccagtattta agaagttggc taatagatag ccgatataag gacctgtggt caagccagaa
    18961 gagcctagac cgctagcagc cagtaagctt ggatcatctg gcagtacgcc gaaaaatggg
    19021 gaaaaatctg acgtataagc acgggtacct acacggaact cgtatggcca gttttttatc
    19081 tgatctggat cagtcaagaa ctgttctgta ctcttagtca atcccttata agcttcgcta
    19141 gtaggagcca aatcccagcc ttgatcgttt tcatgggtag cacctaaaag gatctttcca
    19201 tccatgaaag gaatgaaatc agcttctccg tcaagcatag caacaggcca gtcttgactc
    19261 atcttataag atgtctgaaa tgctaagagc tgcccttttt gcggacgtat atctgcagca
    19321 tagcctaatt cttgtaataa aggcttaagt gccgggccag gtgtcaaaat tacacgatca
    19381 gctgtgtatt ctccaaacgg tcccttgatg agccaagaat cgctcttctt atgcaaagtg
    19441 acttttgcct ggatctgctc catttgtttt tgtgctaaac gctgaccaag atgttctaaa
    19501 tatgcttttc catccagacg accaccacca ctgatacgca gtgaaactgc tggtttcaat
    19561 aacggaagtg cttgagatgt ctgttcaggt gaaagccatt cgatgtttcc aatttctggc
    19621 gcttcttctt ttcgtttctc ggcaagaaca gccaattcag ctaaatcttt ttcaggtcga
    19681 agcaaaagtg tccctgattg tgcgtaaatc gtttgatcaa gttggaaatc ttttataagt
    19741 ttagggaaaa aagcagcgcc gtctttagcc agacgatacc attgtttgtt tctgcgtttc
    19801 gacaaccaag gggagataat cccagcgcta gctttagttg cctgtcctgt cccttcatca
    19861 aataaagcaa cattgaattt tgatgtatct aagtaactgg ctagggtcat accaatgatc
    19921 ccgccaccga taatggcaat tttttccata aaaaagcctc cttttacaaa caaccatttt
    19981 cacacaaaaa aacggcagat gcaacacctg ccgtatatgg aatctacgct tttttagcaa
    20041 agaacagata ataggcgaat cgagcaaagc ttacgagaag caacagcaac aagacgccta
    20101 atgctgcttt tgatcctaat gtagtagaca cttcatagtt atgggaagta tgatgctgga
    20161 cgtaattgat aatgaccgct actaatgaag tagccacagc gccagaaaac tgctgcaagg
    20221 tattgaacat tgcgttcccg tctccttgta gttcttcatt cagtgcattc attccggtgg
    20281 tcatcatatt gctgtaagat aatccgatac cgatcatata aaacacatga caagcaatca
    20341 atacacccac tgaagcgtgt cctatgagta ggaataatgc tagccaacca atcaaagaaa
    20401 gaatcagccc agcaaaaatt ggcttcttcg gaccatattg atccagcatt ttcccggaaa
    20461 aaggagaaag caaagcacca accgctgcgc cgggaagcat cgctaatcca gcgacaaaag
    20521 gactacttcc ttgaacgatc tgaaggaaat tcggcaagac aaaagataca ccaagtaata
    20581 aaaactgata aactaagaag ccaatcaaaa acaagcggaa tgttttattt gaaagaatag
    20641 acagtcggat cagtggatgc tccacttttt tacttcgata gtaaaaaagg aaccagccaa
    20701 taccgccgat gattaaagca atccagctgg aaactgttcc caacgtactc aaaaatgtta
    20761 gacttccact aaacaaaaga caaattccta aaacgcttat ccaatccaaa taaccacttt
    20821 tttgtactgc catctcggga atagcaaacc agccgataat caaagaaaga aacaaaacgg
    20881 gcacaagaaa caggaagata tgattccatg taaaatgtga agtcataatg cctccataag
    20941 tggggccaat tgctggagca atggccgttg tcaaagtacc gacacccatc atcgcgcctc
    21001 gtcgattgag gggggcaaat gtcaagataa tatggaacat caacggaaga gcgatacctg
    21061 tcgctgcacc ttggagcaat cgaccaaaca ataggaccat gaaggtagga gagaagaaat
    21121 cgatcattat acctgccaag aaaagcagat ttgaagctaa aaagagtttt ttgagactaa
    21181 aattgcgatt caaataagta gacagcggaa caatgatcga aatcatcaaa agataaatcg
    21241 ttgttaccca ttggacttgt gcagtcgtta aatcaaactg tctgataaga gttgggaaag
    21301 ttacattcat ggccgtctcg atcaatacgc ccgcaaaaga catcatccct gttgcaatta
    21361 ctgcaggtac taaaagcagc tgtttttgtt ttgtcattta catcacctct aaaaaatttc
    21421 gtaaaaaaag agagtgattt ctattataaa aatagaaacc gcctctctct cgacacgccg
    21481 aaacgtgtta tctgtacgat ttatttactt cacttattct agcacaaact gaaaaatagt
    21541 gtcaacacga ttagccaagt cactcaaaaa gaggtatgat aataaataac taaatggaat
    21601 ttcgcggagg aagaaaaatg gaaaaaattg aatggcgtaa agataagagc tatagtatga
    21661 aaaaaagccc gcaaatacta gaggttccta aaaaatcatt ctttagcatc agagggatcg
    21721 gcgacccgaa tcaggaagac tttaaacgaa gagtagcctg tctatatgca gtcagctatg
    21781 caatacgcat gtcgcctaaa aaaaattggc tgattcctga ttatcagatg tatactgtct
    21841 atccactgga aggacaatgg ggactgcaag aaaagtattt gcatgaacca gtgatgaaaa
    21901 aagaacattt ttcttatcaa ttgatgatcc gacagcctga ctttgttaca gaagcgatag
    21961 cacaagaagc cattcagcgt tctagcaaac ttccggaaga cttgaaagaa caagtagtat
    22021 tcaaaaagat ggaagaaggt ctttgtgccc aaatcttaca cgtcgggccc tatgccgaag
    22081 aaccagcaac gtttgagaaa ttagaagcat tcatcgcaaa agaaggctat gaaagagctt
    22141 ctaaagaaca caaagagatt tacatcagcg atccaagaaa atcagcacct gaaaaaatga
    22201 agacgatttt gcgtgttaaa atagaaaaag gttagcaacc tgaatgaaaa caaaaaataa
    22261 aagtaaaaaa taccaaaata tgaagaaata ttacttatct tttgctataa tcaacattga
    22321 gaggtgtcta atatgaaaaa gaaacagcga caagcactga tccgccagat cattacagaa
    22381 caacctattg ggacacagga agagctgctt gcgcggctgc atgaagcagg cgccgatgtg
    22441 actcaagcaa cgatttctcg agacattcga gaaatgaagt tagtaaaaag tcaaaatgaa
    22501 aacaaaatag tacgctatac attatttaac cagccatctg tttctttaaa tgaagaacgt
    22561 ttgcgtacag caattagacg cgaggttttg cgtatccagt ctgttcagtt catggttatc
    22621 gtattgactg aacgaaatgg tgccgatgtg gtaacgaatt ggctagatga agtggcttac
    22681 cctgaagttg tcggaacgat ggccggtgta gatacattca ttattatttg ccgctcagaa
    22741 gaagaagcac agcgatttgc agaaaagctg gaaaaaatga gggaataaat gaggaggact
    22801 tttatatgaa agatatgaaa gcgctgcaaa atggatcgga tattcgagga atcgcattac
    22861 cagttgaagg atatgaagta aacttgacga cagacatggt aaaaaagatc ggctggggac
    22921 tagcaaactg gataaagaaa aagaagaaat caaccgatcc aaataaaaaa tggacgattg
    22981 ggattggtca tgacagtagg ataagcggac cagcactgaa agaggcgttg atccaaagtc
    23041 tgaaagaaca agggatacat gtcatcgatt tcgaattagc tactacgcct gctatgttta
    23101 tgtccactca atttgaacag ttctcgtgcg atgctgcggt tatgctgact gctagccatc
    23161 ttccctatta ttataatgga ataaaattct ttacctcaga aggaggagca gaaaaagaag
    23221 atattgcttt tatcctatca cagcagcaaa aaataaaaag cgaacaaact ggaagcgtag
    23281 aaaaagcaga ccttttgaca ccttatgcgc aagatttagt cacaaaaatt aaaaagggga
    23341 ttggaacact agaaagcaaa ccactaaatg gttggaaaat cattgttgat gccggaaatg
    23401 gtgctggtgg attctttgct gaaaaagtat tgaatagctt aggtgcggat tcaacaggtt
    23461 cccaattttt ggaaccagat ggccgatttc ctaatcatat cccaaatcct gataataaag
    23521 aagcgatgga aagtatcaag caggctgttt tagcaaatga agcagatctt gggatcattt
    23581 tcgatacaga tgtggatcgt tctgcagttg tcagtgcttc tggaacagtg atcaatcgaa
    23641 ataatttgat cgcagtatta agtacaatcg tattaaaaga gcatccagga acaagaatcg
    23701 tgacgaattc ccctacttct tctcatctac aaagattcat tgaagaaaaa ggaggcatcc
    23761 aagttcgtta tatttccggc tatcgaaatg taatcaataa agcattagaa cttaaccgtc
    23821 ttgggaaaga tacacagcta gccatcgaaa caagcggaca cgctgcattc aaggaaaact
    23881 attttctgga tgatggtgca tatgtcatcg caaagatcct gatgcttttg ccagaattaa
    23941 aaagaaaagg tatcacatta gatgaacaaa tcaaagagtt gaaacagccc gtagaagtac
    24001 aagaaatccg attaaaattg ttcagcgatg aaccacgcga acaaggaaac aagatattag
    24061 aagaaatgaa gcgactgttg gaagaagaaa aagggatgaa cttcgacccg gaaaatgaag
    24121 aaggtattcg atatgatcta gaagagccat acggaaacgg ctggtttttg atccgtttaa
    24181 gcttacatga gccgttacta gtattacaga tagaaaatga tgaattcgga aagaacaagt
    24241 tggcgttagt aaaattagca aaattattcg gacatttttc tgatcttgaa tcattaacag
    24301 cagaataaac cagtcttttt gaaaggctag ttgcttggac ggcaaaaaaa gtggtatggt
    24361 taaatcgagt gctggtaggg agaccagcaa aaggaggaaa aaatggtaac gaatatggtt
    24421 atttttttaa cagtattggt ttatggggtc aactttctgg tatatatgta tcttaaaatc
    24481 aatcatataa aagattatgt agaaaagatt gctatttatt ttggaacaaa tatgacgctg
    24541 ttgttcacga gtagtatttt tttgtttttt ggaaaaatag tagaagacgg cattctggta
    24601 ttcgaataag cagaattttt gtagaaagcg ccttacttgt catacactat aattatagcc
    24661 aagtagcaaa tggtcaatcg aaaggagcag tacaatgaaa aaagcatacg aaacagcaat
    24721 gatcaaccat ggcgggcgca atggagaagt cgaggcacca aatggctcga tgcatatgaa
    24781 aatcactcct ccaggaatcc atgcagaagg aacaaatcca gaacaattat ttgcagcagg
    24841 ttatgcttct tgtttcaatg gggcggtaca acatatgatc aaagaagcag gaatgacagc
    24901 agagtccaca gtaaaagcac gtgtctctct ttataattta gatgatggcg gctatcagat
    24961 cggtgtagta ttagaagtgc atatcgatgg catcagcgaa gaagaagccc aaaaaattgc
    25021 agaagatgcg catgaattct gcccttactc caaagcaact agaggaaata tcgatgtaga
    25081 agtaacgatt gtttagaaga ataaaggaaa tcgaaccaga ctaaaaacgt gaaaggaccg
    25141 ctttttgtct ggt
//
LOCUS       DAFCNS010000039        24381 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFCNS010000039 DAFCNS010000000
VERSION     DAFCNS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.039"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..615
                     /locus_tag="LNE23_001560"
     CDS             <1..615
                     /locus_tag="LNE23_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK6931417.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="LNE23_001561"
     CDS             612..1385
                     /locus_tag="LNE23_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK6931418.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="LNE23_001562"
     CDS             1382..1780
                     /locus_tag="LNE23_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBK6931419.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="LNE23_001563"
     CDS             1965..2636
                     /locus_tag="LNE23_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931420.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="LNE23_001564"
     CDS             2695..3135
                     /locus_tag="LNE23_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931421.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="LNE23_001565"
     CDS             3147..3815
                     /locus_tag="LNE23_001565"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK6931422.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="LNE23_001566"
     CDS             3879..4502
                     /locus_tag="LNE23_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBK6931423.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="LNE23_001567"
     CDS             4558..4944
                     /locus_tag="LNE23_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBK6931424.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="LNE23_001568"
     CDS             5157..6548
                     /locus_tag="LNE23_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBK6931425.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            7159..7527
                     /locus_tag="LNE23_001569"
     CDS             7159..7527
                     /locus_tag="LNE23_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBK6931426.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7707..8648
                     /locus_tag="LNE23_001570"
     CDS             7707..8648
                     /locus_tag="LNE23_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBK6931427.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8717..9448
                     /locus_tag="LNE23_001571"
     CDS             8717..9448
                     /locus_tag="LNE23_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBK6931428.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            9592..11772
                     /locus_tag="LNE23_001572"
     CDS             9592..11772
                     /locus_tag="LNE23_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBK6931429.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11792..12274
                     /locus_tag="LNE23_001573"
     CDS             11792..12274
                     /locus_tag="LNE23_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBK6931430.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            12290..13051
                     /locus_tag="LNE23_001574"
     CDS             12290..13051
                     /locus_tag="LNE23_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK6931431.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            13182..14516
                     /locus_tag="LNE23_001575"
     CDS             13182..14516
                     /locus_tag="LNE23_001575"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBK6931432.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            14823..15308
                     /locus_tag="LNE23_001576"
     CDS             14823..15308
                     /locus_tag="LNE23_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBK6931433.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            15301..15984
                     /locus_tag="LNE23_001577"
     CDS             15301..15984
                     /locus_tag="LNE23_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBK6931434.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            16110..18755
                     /gene="polA"
                     /locus_tag="LNE23_001578"
     CDS             16110..18755
                     /gene="polA"
                     /locus_tag="LNE23_001578"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBK6931435.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            18800..19636
                     /gene="mutM"
                     /locus_tag="LNE23_001579"
     CDS             18800..19636
                     /gene="mutM"
                     /locus_tag="LNE23_001579"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBK6931436.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            19600..20229
                     /locus_tag="LNE23_001580"
     CDS             19600..20229
                     /locus_tag="LNE23_001580"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBK6931437.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            20281..20613
                     /locus_tag="LNE23_001581"
     CDS             20281..20613
                     /locus_tag="LNE23_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK6931438.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            20925..21416
                     /gene="nrdR"
                     /locus_tag="LNE23_001582"
     CDS             20925..21416
                     /gene="nrdR"
                     /locus_tag="LNE23_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBK6931439.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            21439..22812
                     /locus_tag="LNE23_001583"
     CDS             21439..22812
                     /locus_tag="LNE23_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK6931440.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            22812..23738
                     /gene="dnaI"
                     /locus_tag="LNE23_001584"
     CDS             22812..23738
                     /gene="dnaI"
                     /locus_tag="LNE23_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBK6931441.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            23867..24307
                     /locus_tag="LNE23_001585"
     CDS             23867..24307
                     /locus_tag="LNE23_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK6931442.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         8158 a         4172 c         5372 g         6679 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga
     6841 gcaatttcaa aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt
     6901 gtcaggagaa caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt
     6961 atttattcca tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa
     7021 caaccaactt atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc
     7081 tttcgtagat tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga
     7141 tcgtttagga ggaaacagat gtttacgaat gaaataaaaa tcatgcttta tgtggacgat
     7201 gtagaaaaaa atgcaacgtt ttggagagca gttggctttg cggaaaaaga tcgtcaagaa
     7261 atggatggga cgcttataat tgaagtagga gttgaggaca gtcaaacctc atttctgtta
     7321 tatgatcgag catttgttga gtcccattca cctggcacag ctactagtgc cccatctttg
     7381 atgttttaca gtaaagatat ctttggacta tataaaaaaa tgcagacgca atcagtcaca
     7441 ttaggtgata tggtacagct tggagaagaa tatgtcttta actttgctga tcccgatggc
     7501 aactactttg cggttacagg taaataatag aaataaagac tttgtttttc tgtgcgaaac
     7561 aggaaagcga agtcttttta attcaaataa taatattatg taaacaaaaa actttccatg
     7621 cgtaattata cacaagcaaa taaatcgtca aatgcgaacg ccttctttat actgtttagg
     7681 aacgaaaaga aggaaggaag aaatacatgg attatcaagt actgctttat tacaattata
     7741 caaaaatcga agaaccagag gaatttgcca aaaatcattt agccttttgc aaatctttgg
     7801 atcttaaagg tcgtattcta gtagctgaag aaggaataaa tggtactgtg tctggaaccg
     7861 ttgaaaacac gactgcctat atgaaagcaa tgaaagctga tcctcgattt gctgatacga
     7921 tgttcaagat cgatccaagc gaaggacatg cctttaagaa gatgtttgta agataccgtc
     7981 cagagcttgt aagtttaaag ctagaagagg acattgatcc aaaccaagtg acaggtgcct
     8041 atctttcacc gcaagaattt aaagaagcta ttttagatga acagacagtg gtgattgatg
     8101 cacggaatga ttatgaatat gacttgggac attttcgagg ggctgtacgc ccagatatac
     8161 gcagttttcg agagcttcca gactggatcc gcgagcacaa agaagaattt atggaaaaac
     8221 gggtagtgac ctactgtact ggtggcattc gttgcgagaa gttttctggt tggcttgtcc
     8281 gtgaaggatt caaagatgtc ggacagctgc atggcggtat tgctacttat ggtaaggatc
     8341 ctgaagtcca aggagaatta tgggacggca agatgtatgt gtttgatgaa cggatcgctg
     8401 tcgatatcaa ccaagtagat aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat
     8461 gtgaacgata tatcaattgt agcaatcctg agtgcaatcg tcagattctt gcttccattg
     8521 aaaatgaaga aaaacattta ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc
     8581 gctatgttca agaccatgga atctctccag aagaagcaga actgcggttt acagctttaa
     8641 ttaaatagaa aacaatagtt ttgattgtcg accatttcca atatgcgcta tactagcgtt
     8701 gaggatggtg atccacatga aatcattgat ttatgctatt ggggatgtcc atggcaaaca
     8761 tgagcttttt gaaaaactta ttcactatta tgatccaaag atccatcagc ttgttttatt
     8821 aggggatctc aatgatcgag gtcctcgtac aaaagaatgt tttttattag gaaagcaatt
     8881 agtcgaagaa acaggagcca tttacctccg gggaaaccat gaagaatatt ttttgcaatt
     8941 tctgcaatca ccggaggact ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt
     9001 tgaaagcctg attcacccag gggcatcgga ggaatactcc ccaactgaaa tctcgatgat
     9061 gatccaatcg agatattcag aactgattaa atttttagtc cagcgcccct tgtattatga
     9121 atgggaaaat tatctttttg tccatgccgg tgtggatttg acaaagaaag actggcgtaa
     9181 gacaagtcta agagacttta tttggataag ggaaccattt catgaaggga aaaacaatac
     9241 aggtaagaca attgtatttg gacatacgat tacaccaatg ctccatggag atatgcagac
     9301 aacagacctc tggattcagg atcataaaat cggaatggac ggtggggctg tattcggagg
     9361 gtcattgcat ggggttgttt ttgacaagga aggtattctt caagatattg aatatcagaa
     9421 caacgaagga ccttggcatg gaatctgact aatggaaaga atgaaggagc tgtgactttg
     9481 ttgcggctct ttttattttt atagagaata ttgtttcttt cagtagttta acccaggata
     9541 aaaagtatga tagaataaag aagcaatttt tagcagaaaa gaggcatttt aatgacggaa
     9601 aatttgaacc agacgatcat tcagcttgta caaaaagaac tttcagacta tcgtcctaag
     9661 cagttgacta ctgtacttaa tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt
     9721 taccgaaaag aaatgactgg aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg
     9781 tatgcctatc ttgaaaactt agaaaaacgt aaaactgaag tgattcggct aatcgatgaa
     9841 caagggaaac tgactcccga gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa
     9901 gtggaagatt tgtatcgtcc atacaaacaa aaacgacgga caaaagcaac gattgctaaa
     9961 gaaaaaggac ttgaaccact cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa
    10021 tctgaagcag aaaaatatat tgacaaagaa aaagaagtct cttctgccga agaagcgttg
    10081 cacggtgctc atgagattat tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg
    10141 attcggtctt atacgtataa caaaggtatg tatgtcagca atgtcaagga tgagcaagca
    10201 gatgaaaaag gcgtatatga aatgtattat gactttgcag aacccgtcca taaaatggtt
    10261 tctcatcgaa tattagctac taatcgtgga gaaaaagaag atattctgaa agtattcttt
    10321 caagtagatg aagcagcaat tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc
    10381 tctccttctt cttcattcgt cagagaagcc tatcaggata gttataaacg ctttattcag
    10441 ccagcaatcg agcgagaact gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc
    10501 gctattttcg gcgaaaacct ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt
    10561 gtcctgggat ttgatccggc ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca
    10621 gggaaagttt tagcaatcga agttatttat cctcataaac ctgcagcgca agcaaaaaga
    10681 gaagctgctg gaccagcttt tatccaatta attaataaat atcaagtaga tatggtagct
    10741 atcggaaatg gtacagccag tcgggaatct gaactttttg tggctgagca gttgaaatcc
    10801 gccgaccata aaacctatta cgctatcgtc aacgaagcag gcgcttctgt ttactccgct
    10861 agcgaaattg cacgaaaaga atttccgcat ctacaagttg aagaaagaag tgctgtttct
    10921 attgcccgtc gcctgcaaga tcctttagct gaattagtca aaatcgatcc taaagctgtc
    10981 ggagtaggcc agtaccaaca tgacgtttct caaaaacgtt tggctgaaca attggatttt
    11041 gttgtggaaa cagcggttaa ccaagtaggt gtggatgtga atacagcaag cccgcagctc
    11101 ttgcagcata tatcaggatt gaataaaaca actgcccaaa acattgtcat ttatcgtgaa
    11161 gaaaacggag aattcaccgc acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa
    11221 gcatatgaac aagcaatcgg attcttgcga gtccctggtg ggaaaaacat tttggacaac
    11281 acaggtatcc atcccgaaag ttattctatt gcaaaagaaa tattaacttc agtccaactt
    11341 tcagaaaaag agcttggtac agaagaagca gtagaaaaat tgacacgatt atccgtagag
    11401 aaactggctg aaagcctatc agtcggagaa gaaacgttag ccgatatttt agctgggctg
    11461 acacaacctg gacgagatat gcgtgatgag atgccagcgc ccttattacg tacagatgta
    11521 ttgagtatgg aagacttgaa accagggatg gaattgactg gaacggtccg taatgtcatt
    11581 gacttcggcg catttgttga tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa
    11641 ctcagcaaaa aattcgtcaa gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca
    11701 gtttggatag aacaagtcga tacgaaaaaa ggcagaatca gtttgacgat gctttcccct
    11761 tatgaagagt aacaatgagg agtaacaaaa attgacacaa gaagaactgc agcagttagt
    11821 agaagagata tcattaaaaa gcttccagct tcctttcaag caccaagcaa tgttcaatcc
    11881 acgcttacgt acaactggcg ggcgctatca tttaaacgat catcatttag attttaatgc
    11941 gaagctattt gaagagtcag acgaagagac gattgcagga atcatcaaac atgaactttg
    12001 ccattatcat cttcatctag ctggaagagg gtatcagcat aaagatatgg atttcaagta
    12061 tcttttgaaa aaaacaggag ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa
    12121 gatggagtat tatcgatgcc aagattgttc tgccacgatc tgccgcaaga gaagaatcga
    12181 tgtacgcaaa tatcgttgtg gaagatgtca tggagaaatc aaatggattg agacaaaaag
    12241 agaagacttt aatgaagcac aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag
    12301 tgagcagagg aatctaaaag actggaatca gctattaaat tttttagccg cacctgaaga
    12361 gggaaatttt gaggaactga cggcacctac acttatttta gctggaaact gtcttcctat
    12421 cttagctgat aaagcagcac aaatgtattt aaatggccaa gtagatcaat tgtttttggt
    12481 cggaggggtg gggcatgcga ctagaatcct ttatgagaat tttgaaaagc aaggatttca
    12541 ttttgaagat ggcatgagtg aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga
    12601 tttgcccgat aaggcttttc tgatagaatc gaagtctaca aattctggag agaacgccac
    12661 tttttcactg gagattcttc attccttagg agctgtacca gaaaaaatct tattgatgaa
    12721 tgatccgaca ctgcaacgca ggactcgagc aacttttgag aaagtatggc aaaatgaaca
    12781 aactattttc gcgaatgctg tgccatttgt gccggaaatc cttcactttt ctgaagagat
    12841 catctttaca gcaaaagaac tgaatcatca atggcctaag gaatatttcc atgcgctagt
    12901 attaggagaa atggaacgtc tccatgacga tgagaacggc tatggtccga aaggaaaaga
    12961 ttttattcct cacattgata tatcagagga agtttggtct tcttacacca gaattaaaga
    13021 gtcaataaaa acggattttt ctcgtacttg accattttag caagcgaatc ctcctttcca
    13081 aataaatttg gttgaaattt tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa
    13141 tagctaaaag aatggacgct agataagaga agaggattca aatggaaaat caaaataaat
    13201 tgtatcattt tgttggtatc aaaggttcag gaatgagctc cttggctctt gttttacatg
    13261 aacaaggtct aaatgtccaa ggatcagaca ttgaaaaata tttctttaca caaagagatt
    13321 tagaaaaagc aaatatcacg attctgccat ttaacgcaga taatgtaaaa ccagggatga
    13381 cgatcattgc aggaaatgca tttcctgatt cacatgaaga aatccagcga gcaaaagaat
    13441 taggtttgga agtgattcgc tatcacgact ttattggtca ttttatccaa aattatacga
    13501 gtatcgctgt aacaggctct cacggaaaaa caagtacgac tggactgctc tctcatgtat
    13561 tgagtggtgt ccgtccaaca agttatctaa ttggagatgg aacaggacat ggtgatccgc
    13621 aagcggaatt cttttcattt gaggcctgtg aatatcgccg tcatttcctt gcctattcac
    13681 cagactatgc aatcatgacc aatatcgatt ttgatcatcc ggattattat acaagtatcg
    13741 acgatgtatt tacagctttc caaacaatgg ctggacaagt gaaaaaagct atttttgcgt
    13801 atggtgacga tgcttattta cgcaaattaa aagcaaatgt gccgatttat tattatggtg
    13861 tcacagaaaa cgatgatatc caagcacgaa acatcgaacg cacaacttct ggttctgctt
    13921 ttgatgtcta tcatggggat gaatttgttg ggcacttcac tgtaccagca ttcggtaaac
    13981 acaatatctt aaatgcttta ggtgttattg cagtagctta tttcgaaaaa ctggatctga
    14041 aagaagttgc agaagaaatg ctgactttcc caggagtaaa acgtcgattc agtgaaaaga
    14101 tcgtagcaga catgaccgta gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa
    14161 cgattgatgg cgcacgccaa aaatatccag ataaagaaat aattgcagtt ttccaacccc
    14221 atacgtttac gcgtacgatt gcattgatgg atgaatttgc agaagcactt gatttagctg
    14281 ataaagttta cttatgcgat atttttggtt cggctcggga agaacaagga aacgttaaga
    14341 ttgaagacct tggagcgaaa atcaaaaaag gtggagaagt catcaaagaa aataatgtat
    14401 ctccattgtt agattatcat gatgcagtag taatcttcat gggtgctgga gatgttcaaa
    14461 aatttgaaca agcgtatgaa aaacttttaa gcagcacgac gaaaaatgta ctgtaataca
    14521 attactgcag tgtccttata aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc
    14581 cccttttttg caacttatga taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    14641 aatcaaatca aaaacatgag aagctgtttt ttcttggctg ttcttattat ttgatgcagg
    14701 acagcttttt cacgacctct gtaaactata ggaatctgtt aaatcaaggg agcagaactt
    14761 gccttttacg ctacttttgg tacaataatg tatgattaaa aataaactgg agggtttcta
    14821 tcttgaacat aggtaagccg agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg
    14881 actcgttatc cttaacagaa tctattgaag ataaccagct tttgctgtat ttgggattga
    14941 cgaatgatgc aaatcctttg tatatccaac atgactttgc acaaaaaaca gaatatcaac
    15001 agccaatcgt tccatcagtg atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc
    15061 ttcctggacc aggatcacat gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc
    15121 attatgaaac actgactttt cagtttgagg tcataaaagt agataagatg aaggatgtgg
    15181 tgacgatttc tgtagaagcg gagaatacga acgaagatcg tgtgttggat gctgtcgtta
    15241 tggtccaacc gccacaagtt cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga
    15301 atgaataatc caagtacatc aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga
    15361 tttttaggta tgggacttgc ccttagtgca gtcacttctt acttggctct ttgggtattt
    15421 cctttgcaat tagcaacttt tatcaataat tttccactag gctttgtggg tctgtggatc
    15481 gttgaactga ttttagtaat ggttctaggc gtaaaagcac aaaagaatcc gagcttagca
    15541 atcggtggat ttattgccta ctctcttttg aatggcataa cattagcggt tactcttgca
    15601 tactacgata ttggaacagt aacgcaagct ttcttgacag ctactttgat gttcatgggg
    15661 atgtctgttg tcggagtaac aacaaaaaga gatcttagcg gggttggaag agcaggacag
    15721 gcagctttaa tcggagtgat cattgcacta gtcttgaaca tcttcatttt gagaagtagc
    15781 gtagttgagc tgttcatctc cctactgtta gtcgtcatct ttgcagggat tacagcttat
    15841 gataaccaaa gcatccgtaa tatctatgtc cagacaaacg gaacagcaca tagtggaatt
    15901 gctgtattca tggcattgaa gctgtatttg gactttatca acttgttctt ggcgtttctg
    15961 agaatctttg gacggaacaa ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa
    16021 ttatttggta tggtcccgct ttttcttttc gcattccgca ggattctttg ttagaatgaa
    16081 taagaacttt acagtaagga gttaagaaga tgacaaaaaa taaattatta ctcgttgatg
    16141 ggaacagtgt ggcttttcgc gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa
    16201 atagaaacgg tcttcataca aatgcaatct atgcattcaa tacgatgttt gaaaatgtta
    16261 tgcaaaaaga gcaaccgact catgttctgg ttgcgttcga tgcaggaaaa acgacttttc
    16321 ggacagaaat gtatgcggag tacaaaggtg gaagatccaa aacacctggc gaattcaaag
    16381 aacaaatgcc atatatacgt gatttgttga caggactagg ggtacagtat tacgaattgc
    16441 ccaattatga agcagatgat attatcggta ctttggcaga aaaagtagac aaagatcaat
    16501 ttgatgtcgt cgtgctttca ggtgatcggg atttgactca attagctagt aaagaagtaa
    16561 aagtggatat cactgtaaaa ggtgtcagcg atatcgaaag ctatacacca gaacatgtag
    16621 cggaaaaata tgatgggttg acgccaaaac agattattga catgaaggga ctagccggag
    16681 atgcctctga caatatcccc ggtgtgacta aaatcggtga aaaaacagct atcaagctat
    16741 tgaaacaata tggctcagtc gaaggaattt atgaacatat cgatgaaatg aagcaaagca
    16801 aaatgaaaga aaatctgata aatgacaaag agcaggcttt cttatcgaaa aaattggcga
    16861 ccatcaatac ttcagcgcct gtggatgtct ctatcgactc attgaagtat gaagggaaaa
    16921 atctcgataa actcgttccg ttttataaag aaatggattt caatcagttc ctttctaaat
    16981 tgaatatcgt agaagaagaa gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat
    17041 tcaaagagga aatgtttact acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt
    17101 atcatacaga ggaaatcgtc ggagtagcgt gggggacaga aaagaaaatt tatgtcacga
    17161 acgatctatc tgtattcgaa aaccgagcgt tccattcttg gatcactgat ccgactcggc
    17221 tgaaaaaagt atatgatgcg aaacgaactt atgttgcttt aaaccgctat actggaaaaa
    17281 cgaaaggaat cgattttgat gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa
    17341 gcacagatat cgaaggaata gctgctcatt acggatacaa cgatatccaa tcagatgaag
    17401 ctgtatacgg caaaggggcg aaaaaaggtt taccagaaga agaggaactc ttttttgctc
    17461 atttagcgcg aaaagtagct gcaatcaatg cgttgacacc aaaattgagc caagaactgg
    17521 cagaaaaaaa ccaagaagat cttttccgga aaatggaact gccattgtct atcattttgg
    17581 cagagatgga aataagcggg atcaaagtag atgcgactcg cttacaagaa atgaagggcg
    17641 agttctctgc tcgcttaaga gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat
    17701 tcaatttgaa ttcaccaaag caattaggag tgatcttatt tgaaaaaatg ggattacccg
    17761 ttatcaaaaa aacaaaaact ggatattcca cagccgtaga tgtactggaa cagcttcgtg
    17821 aacaagcacc aatcgtagag gatatcctca cctatcgtca aattgcaaaa attcaatcta
    17881 cctatgtcga gggactgctg aaagtgatcc aatcagatgg aaaagtgcat acccgttatg
    17941 tccagacatt gacccaaaca ggacgattaa gctctgttga tcctaatttg cagaacatcc
    18001 cgattcgatt agatgaaggg agaaagatac gtcaggcatt tgttcctaga gaaaaagact
    18061 ggctgattta ttcttctgac tattcacaaa tcgagttacg cgtacttgcg catatctcaa
    18121 atgatgaaca tttgaaagct gccttcctag aaggtcaaga tatccatgcc agcacagcga
    18181 tgcgtgtatt tggaatcgaa aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa
    18241 aagcagtgaa ttttggtatc gtgtatggca tctctgatta cggactttca caaaatctag
    18301 gaatcagccg aaaagcagca cagcaataca tcgatacgta ctttgaaaaa tacccaggtg
    18361 tcaaagaata tatggaaaga atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc
    18421 tgtatcaccg ccgtcgatat ctgccagaca tcaattcaag aaattacaat atccgttctt
    18481 ttgcggaacg aacagctatc aatacaccaa tccaaggaag tgctgctgat attttgaaaa
    18541 tcgcgatgat cgaattggat aaacgattaa aagaaacagg tcttcaagct acgatgctgt
    18601 tacaagttca tgatgaactt gtcttcgagg taccggaaaa agaacttgaa tcattagata
    18661 aattggtgaa ggaagttatg gaacaagctg tttctcttca tgtaccactg atcactgaca
    18721 gcagttgggg aaaaacgtgg tacgaagcaa aataaacatt gtttaagaca gagcaatata
    18781 atgaaggtga aaagaaaaaa tgccagaatt accagaagta gaaaccgttc gtaaaggatt
    18841 ggaacggtta gtagtaggaa aaacgattca aaaagttcag gtcttgtggc ccaagatcat
    18901 cgagcaacca gagacaccca tctttgaggc ttctcttgtt ggcgaaacca tccaatccat
    18961 tggacgtaga gggaagtttt taatttttca tctcgatcat tgtgaactga tctcacactt
    19021 gcgaatggaa ggaaaatatc aatttacgaa agaaaataca ccaatcgata aacatacgca
    19081 tgtacttttc ttttttgagg atggaagcca acttcgctac aacgatgtac gaaaatttgg
    19141 tcgtatgaca atcgtcgaaa aaggagcaag tgcaacttac cgaggaatca tgaaattggg
    19201 acctgagcct ttgccagata gcttcttatt agcagatttc gcaaatggac tgaaaaagtc
    19261 ccacaaagca atcaagccat tgctgctaga ccaacgtcta gtgactggtc taggtaatat
    19321 ttatgtagat gaagcattat gggaagcgaa gatccatcca gaacaaccgg cgaacacact
    19381 gcgccccaaa gaggtagaac aattacgtct atcgatcatt gatgttttgg atcgtgcaat
    19441 agaagccgga ggtaccacga ttcgcagtta tttgaatgca ttaggagaat caggcggatt
    19501 tcaagtagca ctgcatgtct atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc
    19561 gatcgtgaaa acaaaagtgg cacaaagggg gacgcactat tgtccgaatt gtcaaaggct
    19621 aaaaatcgca aagtaggatt tgttttaggt ttgacgggtg gaatagctac cggtaaaagt
    19681 acggcagcca aagtctttca gtctcatgga tttcctttga tcgatggcga tgtgattgcc
    19741 agagaaactg ttgagcctgg aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa
    19801 gaggtattgc agcctgatgg gcaactagat cgagggaaac ttgggatgat tgtttttcca
    19861 tcaaaggaaa aacggcaaaa acttgatcag ctattagatc cgtttatccg aaaagcgata
    19921 aaagatcaaa tcgcctctct aagttccaat catcctttag taattgtaga tatcccattg
    19981 ctctacgaag gtcattatga ccactatatg gatgcagtag ctgtcgttta tactacgcca
    20041 gaaactcagc ttcagcgttt gatgaaacgt aatcaactga cgctagaaca agcgcagcag
    20101 agaattagta gtcaacttcc aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac
    20161 aacaatggaa caaaagaaaa acttgtggga caaatcgaaa attggctttt tgctaatcgc
    20221 tttctttgag tatctgtgtc aattttgcta tcctgagtat atagaaagtg agggatagcg
    20281 atgggatcat taaaaaatca aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc
    20341 gagatgctca ataaagaaaa tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa
    20401 gaaaaagaaa agcagtacca agcaagaatc gatgaaatag aagaccagca ggcagaagaa
    20461 gaagcgaagc ggcaagattt gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa
    20521 acaccgctcc cagaaagtga ccacattccc gatgagcaaa aaatcgagga acagcgaatg
    20581 aaacactata caggaatcga agagaagtta taaaatagta aaaaatcgag atgtgacttg
    20641 aagcagatga gaaggtgttc atctgcttca agttttttgt gctagatgac taaatcagct
    20701 tttgtgtaag ttatttctca gtaatcaagg tgatcagtat tggaaaattg aaatttagta
    20761 aggacagggg acacaattac gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt
    20821 tgggtaggac agtcgcgttt gtgatttgtt tcgtgttata atacaactag ttgtaaagaa
    20881 agacacatcc tttatataaa atgcgatgca agaggtgaga caaaatgaga tgtcccagat
    20941 gtcaacataa taattcacga gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc
    21001 gtcgccgtcg cgaatgtgag aattgttcgt tccgattcac tacatttgaa cgaattgaag
    21061 ctgctccgtt attagtaatc aagaaaaacg gggaacgcga agagttcaat cgcgataaaa
    21121 ttttaagagg actcatccgt tcagcagaaa aaagacctgt agctatggaa caaatggaac
    21181 aaatcgtcaa ccatgtcgaa aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga
    21241 atctaattgg tgaatatgtg atggaagatt tagttgattt agacgaaatc gcttatattc
    21301 gttttgcaag tgtttatcgc cagttcaaag atatgtctgt tttcttaaaa gagttgcaag
    21361 atatcgtgga taaagcaaaa gaaaataata aagagaataa taaagaaaac aattaagaaa
    21421 caaaaaggag gcatttcctt ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac
    21481 caaggaagaa aagcggctcc gctgagagaa gacagcaaga acgccttgct gtatctttat
    21541 cagccactaa tcggcggaga agccttgagt atctatttca ccctgttatc agaaatctct
    21601 caagaatctg gcaaaggtcc tgaagggatg catgcagatc tgcttagtag tctaggctgc
    21661 ggtatcccgc aattttatga tgcaagaaaa aagctagaag gaatcggctt actggatgtt
    21721 tattttaaag aagatagtga tctgggacct cgttttattt atgagctgtt agaaccactc
    21781 attcctaaag catttttcaa agactctgtt ctggcttttc ttcttctgga aaaagttggt
    21841 gaacgacgat tccagaagct tacccaattc tttgaaccta aaaggcatac agcagaggga
    21901 tatcaaaaaa tcacaaaaaa atttttagat gtttatcgct tcaatgaagc agtgtacgct
    21961 gctaatcaag aaaacatcga atcgctgaat aaatcttttg aagaatctga atcagtagca
    22021 ttacttgaag cagattcgac attcgattgg cagtttttga atgattggct ggcaaaaaaa
    22081 catattgcaa gtcttgatga tccaatcatc aaacaagtcg acatgtatca tcagctttac
    22141 ggtttcgatg aattgacact tggcgaactg atcagtcaat cttatgactt tagtgaacaa
    22201 aaagtatctg aaaaagaact gcaaaaagcc attttggcaa tgcagagacg tccggtgaag
    22261 caaacaaaag aaacagttgc ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat
    22321 ttgacaaacg cagcgcttca gctgataaaa gaagcacaga gcattccacc gatgaaatat
    22381 ctggaagcaa tcaagtcaga aaaaggcgga tatgtttcta aacaagaaaa ctggttactg
    22441 caagatcttg tttctcaatc aggtctttcc agtagcgtga tcaatgtttt gctgaattat
    22501 gtactagtga tcaagaataa tgctagctta aatgccagtt atgtaaatac agtggcaaat
    22561 gaatgggcac aaaagaaaat cgccacagca gaagaagcga tccagcacat tcggcaaata
    22621 agtaatcaag caaaacaatc aaaacaaaag aagcaaacaa attatcaaac tggcaagcga
    22681 aatgttcgac gggaaaagct tcctgattgg gtcaaccagc caaaagatga aaaacagatc
    22741 agcagcgaga aaaaagcaga gattgaccgc cgttttaaag aatacctggc aaaaaaggag
    22801 ggtgatatct agtggaagac gtaggcaaag aattgaagag gatcatcagc aaacgcaatt
    22861 atcaagaaaa gtatgctgaa atgatcgctg aagtcttaaa tgatgcggat gttcaagctt
    22921 ttctaagcgc acaccaagaa gaattgacac aagcggatat tgaaaaaagt tacgctaaat
    22981 tgtatgaatt cgtgcaagaa aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc
    23041 ccggctatga gccgaagctg acgttgaatt ttcattatgt ggatgtcact tatgtaccaa
    23101 cggaagcatt gttagcacgg caaagacaag aagaaatcag aagccgagta aaagcaatgg
    23161 atatgccaaa agatattcaa tcggcttctt ttgaaaactt tgaacgtaca gatggacgag
    23221 gatatgcgag cttagaagcc ctagattttg tagagaaata tactgctgat ccaaaagggt
    23281 atcataaagg actgtatcta gtaggaagtt ttggtattgg aaaaacatat ttattaggag
    23341 caacagcgag agaattagcg attgctggat tcaccacgac gctagtccat tttccgacat
    23401 ttgccgttga gatgaaacag gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag
    23461 tgaaacgttc acctattttg atgatcgatg acattggtgc agatgcgatg agtagttgga
    23521 tacgagacga agtattcggt gttatccttc aatatcggat gcaggaacag ttaccaacct
    23581 tcttctcatc gaattttaca atggatgaat tggaacagca tttgtccgtc actcaacgtg
    23641 gagatgaaga gccgctaaaa gcgaaacgga taatggaacg catacgttat ttgacaaaag
    23701 aaatcgaaat gactggtcgc aaccgacgca atggttgaac agttgacaag tccaactgac
    23761 atttgaccta gtagtaaaag caggtcagcg aatcattata tggacagaaa tgaccggaga
    23821 caatcaccgc aacaagtaat gcagaaactt aaacggagga ttttttatga gggctatttt
    23881 ttctagtata tctgtagttg gtctattttt tcttttagca ggctgcggta gcgcagatag
    23941 caacacaggt tctgttgatt cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa
    24001 aacggagaga caaagcatat ttgtcggaac gattgaaagt atcactcaag tagaagcaga
    24061 agaagaaacg cttcagttga aactcgtcga tgtcatggcc aaagaagatc cagcgaatat
    24121 gggaacgtca ttcagcaatg acggagtgat cttaaatgct actgctgaaa aaataagcgt
    24181 ggacataaat gaattgaaaa tcggtgacaa agtccaattt acattagttg agatgccgat
    24241 catgacgatg tctatcccac cgcaagttcc tggagattcg attattgatg tttcattgaa
    24301 caaataacca ataacatttt ttcaaaaata agaaaatgtt gttttataga acgaaaaggt
    24361 atatttttta aggagatatg g
//
LOCUS       DAFCNS010000040        24270 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFCNS010000040 DAFCNS010000000
VERSION     DAFCNS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.040"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..571
                     /locus_tag="LNE23_001586"
     CDS             <1..571
                     /locus_tag="LNE23_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6931443.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            575..1216
                     /locus_tag="LNE23_001587"
     CDS             575..1216
                     /locus_tag="LNE23_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931444.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1213..2130
                     /locus_tag="LNE23_001588"
     CDS             1213..2130
                     /locus_tag="LNE23_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6931445.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2127..2798
                     /locus_tag="LNE23_001589"
     CDS             2127..2798
                     /locus_tag="LNE23_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931446.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3339..3827)
                     /locus_tag="LNE23_001590"
     CDS             complement(3339..3827)
                     /locus_tag="LNE23_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBK6931447.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4028..4294)
                     /locus_tag="LNE23_001591"
     CDS             complement(4028..4294)
                     /locus_tag="LNE23_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBK6931448.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4294..4557)
                     /locus_tag="LNE23_001592"
     CDS             complement(4294..4557)
                     /locus_tag="LNE23_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK6931449.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5033..5218
                     /locus_tag="LNE23_001593"
     CDS             5033..5218
                     /locus_tag="LNE23_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6931450.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5360..7183)
                     /locus_tag="LNE23_001594"
     CDS             complement(5360..7183)
                     /locus_tag="LNE23_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBK6931451.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7781..10486)
                     /locus_tag="LNE23_001595"
     CDS             complement(7781..10486)
                     /locus_tag="LNE23_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBK6931452.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10792..11739)
                     /locus_tag="LNE23_001596"
     CDS             complement(10792..11739)
                     /locus_tag="LNE23_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBK6931453.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11929..12198
                     /gene="rpsN"
                     /locus_tag="LNE23_001597"
     CDS             11929..12198
                     /gene="rpsN"
                     /locus_tag="LNE23_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK6931454.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12374..12736)
                     /locus_tag="LNE23_001598"
     CDS             complement(12374..12736)
                     /locus_tag="LNE23_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK6931455.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12747..13868)
                     /locus_tag="LNE23_001599"
     CDS             complement(12747..13868)
                     /locus_tag="LNE23_001599"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBK6931456.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13884..14234)
                     /locus_tag="LNE23_001600"
     CDS             complement(13884..14234)
                     /locus_tag="LNE23_001600"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBK6931457.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14364..15875)
                     /locus_tag="LNE23_001601"
     CDS             complement(14364..15875)
                     /locus_tag="LNE23_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK6931458.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16197..17549)
                     /locus_tag="LNE23_001602"
     CDS             complement(16197..17549)
                     /locus_tag="LNE23_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBK6931459.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17702..18778)
                     /locus_tag="LNE23_001603"
     CDS             complement(17702..18778)
                     /locus_tag="LNE23_001603"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBK6931460.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18974..20719
                     /locus_tag="LNE23_001604"
     CDS             18974..20719
                     /locus_tag="LNE23_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBK6931461.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(20999..21436)
                     /locus_tag="LNE23_001605"
     CDS             complement(20999..21436)
                     /locus_tag="LNE23_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBK6931462.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21420..22793)
                     /locus_tag="LNE23_001606"
     CDS             complement(21420..22793)
                     /locus_tag="LNE23_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931463.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23115..23603)
                     /locus_tag="LNE23_001607"
     CDS             complement(23115..23603)
                     /locus_tag="LNE23_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6931464.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23625..24227)
                     /locus_tag="LNE23_001608"
     CDS             complement(23625..24227)
                     /locus_tag="LNE23_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK6931465.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5163 c         4488 g         7660 t
ORIGIN      
        1 agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata caccagaaaa
       61 tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag atcgcctact
      121 acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag caatcacgat
      181 tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac gtgtagatac
      241 gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa
      301 taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg ttttttacgt
      361 aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata
      421 cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg catcattagt
      481 ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg ttgcagagtc
      541 agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc atcagcacgg
      601 ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg ctttgatcat
      661 tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa aagctgccac
      721 gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt tattggcatt
      781 gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct ttatttattc
      841 attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt ggaattatcg
      901 ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg ttgaattgcc
      961 cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg tgattgcatg
     1021 ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt ttagtggact
     1081 gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa ttttagcact
     1141 attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg cacttagaga
     1201 ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc tcttcttata
     1261 cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac catttcgatc
     1321 acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg aatggttgaa
     1381 cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac tatcaaccat
     1441 caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg aacagattta
     1501 acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa
     1561 gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatggttt tgaaaatacg
     1621 tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa agtcagcgat
     1681 ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat caatcgtaaa
     1741 ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc gatattacct
     1801 atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat tgttcttggc
     1861 tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga tgatcgtcaa
     1921 ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc tgatacgcct
     1981 catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa aatgcaggaa
     2041 ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca acgtttctta
     2101 caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat gtctttcaac
     2161 aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt atccgtcact
     2221 tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg gttgggatca
     2281 tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat atcatccaga
     2341 cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg ggagtcaatg
     2401 tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat acgtataccg
     2461 ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg atgactgcta
     2521 gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg gcaggcataa
     2581 gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg
     2641 gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg attattttag
     2701 caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta ggtattttgg
     2761 aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa aaacaaaagc
     2821 ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt aaggcaataa
     2881 tccactctga ttcttccctc cttatgggca aagagttgct tttcttagct atttatccat
     2941 tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc gactaagttt
     3001 ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc atggaattca
     3061 taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc aattcttaca
     3121 tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt cgatgccttc
     3181 agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct tttatacaat
     3241 cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca gcttctcgcc
     3301 atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca tccgtcgcaa
     3361 ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac caattgatcg
     3421 atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg tgatgcattg
     3481 ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc cgtttgagtg
     3541 acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac gacactggta
     3601 ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa
     3661 taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc tgtttctcga
     3721 taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt cgttgccact
     3781 gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact tcttcctcct
     3841 tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc cttcaaaggt
     3901 ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt ggttttagaa
     3961 caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa ggttggctct
     4021 tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta ttttcctcat
     4081 caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg
     4141 taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc aatgtctgaa
     4201 tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa tccactttcg
     4261 cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat caatatcatc
     4321 gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta aagtacctgt
     4381 ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa tcaatacagc
     4441 attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat ttacctcttt
     4501 caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat tagccattgt
     4561 ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca aaaaaataca
     4621 ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa agaccccttt
     4681 ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg gtagccaaac
     4741 gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt
     4801 ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga ttgatatctt
     4861 gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg attttggccg
     4921 tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat tcatgttcac
     4981 tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa aaatggcact
     5041 gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt atgaagacac
     5101 atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct gtacattatt
     5161 tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt acagctaaat
     5221 aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata gagggcacct
     5281 ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag agggtctgtt
     5341 tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg acaacgatat
     5401 tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg agaatattct
     5461 gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag ttttgtttcg
     5521 ctgcattttt acttacgata tctacataac ggataattgg attttcaatc gagcgataag
     5581 aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct gcttcgattt
     5641 ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca taatcaccga
     5701 tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg actaatacaa
     5761 gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga agctgttctg
     5821 ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt ggcataatga
     5881 tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg attgcatatg
     5941 agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt ccttcttttt
     6001 tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg ccgatagagt
     6061 ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg atcgaaccta
     6121 gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct tgatacatgt
     6181 gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca gaaaaaccgg
     6241 tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa tacaaaatac
     6301 catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt tcaggaacga
     6361 tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca
     6421 tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat ggtacagcat
     6481 tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct cgtaaaatca
     6541 aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc aatggcacag
     6601 cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca aggataaatg
     6661 tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga ttcagcatca
     6721 tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt gctggcaccg
     6781 ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac atgtagtcga
     6841 tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca ctgctgacga
     6901 catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat agcgttaatg
     6961 aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg gctgcagcag
     7021 acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat gataacgcat
     7081 cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca ttttcagcag
     7141 attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa actcctttac
     7201 acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat gttatagtac
     7261 gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa acagaaaaat
     7321 atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt tatgggcttt
     7381 ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt tctatccttt
     7441 ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc attcttacta
     7501 ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct
     7561 gcggtaataa gctcaaccaa attaaaaaca taagaagctg tttttatttg gtcgttctta
     7621 ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca atcagctgac
     7681 cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca tttttttctc
     7741 taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa atatggctct
     7801 catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag attgccgttc
     7861 ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc gtagcatttg
     7921 gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta aatggcaaga
     7981 acggattgat catttggaag aatttccctg agacttggat cggatagttc ccgcctccgc
     8041 cagagatcga cagtactaag atgataatag cgatcccttt accgacatta tcgaataatg
     8101 cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca aatgacacgc
     8161 tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat gtaacgatca
     8221 agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt
     8281 tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca acacttgaaa
     8341 gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta gctgaaccgt
     8401 tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta aagaagtcac
     8461 tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg acttcttgtt
     8521 cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt ggccagtcat
     8581 tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt ttttcattta
     8641 ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt tggataaaat
     8701 cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat aggtctgccc
     8761 cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta ttggcatcat
     8821 ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt gccactgcat
     8881 tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc
     8941 tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga gaagtgacag
     9001 tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta ttaacaacgg
     9061 catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga acagctgtgt
     9121 tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga tttagctgat
     9181 caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc aggaactcga
     9241 ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg ttcaatacat
     9301 tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct gttcttacag
     9361 tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat gtaacatcta
     9421 cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat tttgtttggt
     9481 ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata tcttgtactt
     9541 gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt gtctcttgga
     9601 tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg atctctggga
     9661 ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc attttgtcat
     9721 tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc acgaattcat
     9781 ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg aggacttgtt
     9841 ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata tctttaactt
     9901 tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat actttcaata
     9961 aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc gaacttgctc
    10021 ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta tattcgattt
    10081 ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa tcctttggca
    10141 agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt tcttttgaat
    10201 caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact tgctccccaa
    10261 tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca gcaattggca
    10321 gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac gcatatagcg
    10381 aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt
    10441 tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt ctcccccttc
    10501 ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat cggctttttt
    10561 caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc tcttacatat
    10621 tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg aaagataatt
    10681 ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca ttttttgtct
    10741 ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt cttatttttc
    10801 gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat gtgcttggat
    10861 gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg ctcctttttg
    10921 gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc ttggaacaaa
    10981 cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt catttaatgc
    11041 aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga tacttccttt
    11101 cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat ttgttttatc
    11161 ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt tactggatgc
    11221 actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg acccgaccgc
    11281 acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca aatggttgat
    11341 tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc aactcatttt
    11401 atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt aagtcccgcc
    11461 gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg aaccaatctc
    11521 tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg ttcgtatctc
    11581 tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg cttttatttc
    11641 tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt caaaaacatc
    11701 ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct ccctttttct
    11761 ttaagtttac gtttatgtct ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg
    11821 attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta tctaaaagcg
    11881 taaagattac gatttacgct aataaaaaat aattggagga atttgttgat ggctaaaaaa
    11941 tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc tgctttacgc
    12001 caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt agatgctcgt
    12061 ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata catgcgtaaa
    12121 ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat tccaggagta
    12181 aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg cgcatggacc
    12241 actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc tggtactaga
    12301 agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag gctgcatggt
    12361 tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca ctaattttca
    12421 atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt tctttcaatc
    12481 ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt tctattcctg
    12541 ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt tttgccgtta
    12601 tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga ataaccaaaa
    12661 ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca aaataaatat
    12721 ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat ccgctgtcct
    12781 aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc ctgcatagtg
    12841 atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg aaattcttga
    12901 ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca gtagttccct
    12961 tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata accgatcggt
    13021 atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc gattccttca
    13081 cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat
    13141 ggataaactt ccggtagtgc gtgcccagag ggattaagtc cgtacattgc tactccataa
    13201 cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt gctgacatgt
    13261 acatatcgag gaagttcggg aagttttttc agcacttcta taaagcgttc tttttgcaga
    13321 ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa accttcccat
    13381 tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc aggtgtacaa
    13441 aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg cgtctctagc
    13501 ttacttttct ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc tgcagttact
    13561 gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt caagataagg
    13621 atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt tgaaacacaa
    13681 aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc atgaccgtat
    13741 ccgttcgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc aacttcattt
    13801 gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg gtgccaagcg
    13861 acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa agctgtttca
    13921 tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt cacaacaggc
    13981 tgaccatttt cattttttaa aatctcgaca tcttgaaagg taacttttcc gatcccagtt
    14041 ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg
    14101 cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat tcgtgcaatg
    14161 aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc atcgatccca
    14221 attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc attctaccaa
    14281 attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt ttctcttaaa
    14341 aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt
    14401 ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct ataaccgcct
    14461 tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt tccgccacca
    14521 cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc tggtgtgatc
    14581 ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa
    14641 tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt atctagacca
    14701 ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc tttgaatgct
    14761 tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca
    14821 tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc ttttccggca
    14881 cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat atcatagttg
    14941 tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt cgctacaagg
    15001 atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa
    15061 tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc caattcgtct
    15121 acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac atcaaacaag
    15181 cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg atcgatcaag
    15241 tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat gaatttaacg
    15301 ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca
    15361 ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa catttcatct
    15421 gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg
    15481 tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa gccacggatc
    15541 tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt gtcgcgccct
    15601 aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc gataactaaa
    15661 ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc aaatgcagct
    15721 gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa tgctagtgga
    15781 atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac
    15841 aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata tggttgttaa
    15901 ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt
    15961 tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc accgaaaaat
    16021 cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct ttatcattat
    16081 tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt tctcttcatc
    16141 tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac agatttttat
    16201 ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct gccttttagc
    16261 ataatactat ctgttgctgc aagattttgt ttgatagcat caatcaattt attcttttct
    16321 ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc ttttagtgca
    16381 tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat atgttcactc
    16441 atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc taacacagcg
    16501 attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc catggctgtt
    16561 ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt tagccattcg
    16621 gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg tatggacagt
    16681 ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata acttccaggc
    16741 agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt tccttttgct
    16801 gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt ttgtactaat
    16861 gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa tccgtctgat
    16921 acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga cccaagattt
    16981 tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc cataagtgaa
    17041 agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt ttctgtatct
    17101 tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc
    17161 ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt ggtggtttta
    17221 ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta ataggttgcc
    17281 aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt ttttggagct
    17341 tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc ttgctggata
    17401 aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc ggttatcttc
    17461 cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct gcccacggct
    17521 tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa
    17581 agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat ttctctattc
    17641 gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc tttatttctc
    17701 tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt cctaactgga
    17761 tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag agtgggtaca
    17821 tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat tcatttttat
    17881 ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg taagctaact
    17941 tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga atctgcattt
    18001 cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct tttacgactt
    18061 caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg atttcgcgcg
    18121 cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt gctaaagcat
    18181 tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc atattcgccg
    18241 gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact tttttaggat
    18301 tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca ccagcagctt
    18361 gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat acgcctgcgc
    18421 cgacataagg catattcaat gtctctaaga agccttggat cgttccatct tccccgtttg
    18481 gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc ggattgatca
    18541 cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat
    18601 ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct tcttttgtaa
    18661 taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact gaaaatgcgg
    18721 aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta atcttcaaaa
    18781 gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta gcacaaaaca
    18841 aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga acgagcgtca
    18901 tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat gataaagaaa
    18961 aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt
    19021 gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa
    19081 aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc tttctgttcg
    19141 agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag atagtgtacc
    19201 tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc tttcattatc
    19261 agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg ggcaggctca
    19321 tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc ctaacatcgc
    19381 cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga tcaaatacat
    19441 tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac gttccttgat
    19501 caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc aaaagaacaa
    19561 tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga aaaaagtgat
    19621 cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg aaaacaaaac
    19681 tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg caaagctttt
    19741 agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg atcctcatcc
    19801 agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta gccgaaaaac
    19861 tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca cttctgcaga
    19921 agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg ggcatctttc
    19981 tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga aagatgctta
    20041 tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg
    20101 gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta ttgatgaaat
    20161 caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata atctgcagtt
    20221 cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg tgatccgcgt
    20281 cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac attattttct
    20341 cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat tggaagggat
    20401 gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt ggacttcggg
    20461 acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag
    20521 tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag ggtcctccct
    20581 gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag ggatatttac
    20641 gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga tccaaatgta
    20701 taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca agaaataaga
    20761 aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga
    20821 ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata
    20881 agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc cctttttgat
    20941 gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt ggtaaacctt
    21001 agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc gtaaagggtt
    21061 ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa cgttgttcta
    21121 gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac actcgcacac
    21181 ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag tcattcttct
    21241 taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg tagacgattt
    21301 ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg ataaccacgt
    21361 caagaccata ggattctcct aattttatga catcttcttt tactggagaa ccatctccat
    21421 caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt tactaatccg
    21481 ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac actttctatc
    21541 ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc catttctttt
    21601 gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc tttattttga
    21661 gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt ttgataatat
    21721 tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa gacacctatc
    21781 gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag caattgcata
    21841 gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt tgctgcaatc
    21901 aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc gtacagtatg
    21961 ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt tagaaaagta
    22021 aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa aacaaacacg
    22081 aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct ttcatcgaat
    22141 aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa taaaacaagc
    22201 tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta acgcggataa
    22261 tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca
    22321 atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca gatagctgca
    22381 aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg aaaattgacc
    22441 gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact tgaaaaataa
    22501 aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc accaatcagc
    22561 agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt gactaaaaat
    22621 agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag ccaattatgc
    22681 gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt acttaagata
    22741 aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa catgacaatg
    22801 gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa ggggaaataa
    22861 accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt
    22921 ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca aaagtcttgc
    22981 ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg tgcctccaaa
    23041 atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa tttactttaa
    23101 cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat ttgaaatcct
    23161 tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt ataaacctcg
    23221 attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcctt tgtttgcgca
    23281 gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg tactttattt
    23341 agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc agtgtaaccg
    23401 acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag ttctttttga
    23461 atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg accaatgatc
    23521 ggcacaaaag cttccggcca tacttcttgt atgactgcca tcagctcatc cagttcattt
    23581 tctgttactt ttattttttc catacaccca cgtccttttt tacattataa atgaccttgc
    23641 gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca tggtttcaag
    23701 aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc tactagacag
    23761 atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt tttaatatct
    23821 ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga taaaataagc
    23881 tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc cccaatcact
    23941 aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat gcccgctact
    24001 gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa cagaacaaat
    24061 atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt gctcgatcga
    24121 aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag accaataatg
    24181 ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta tattccccac
    24241 atcttttttc atttattaaa actgcctcct
//
LOCUS       DAFCNS010000041        23828 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFCNS010000041 DAFCNS010000000
VERSION     DAFCNS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.041"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(53..787)
                     /locus_tag="LNE23_001609"
     CDS             complement(53..787)
                     /locus_tag="LNE23_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK6931466.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="LNE23_001610"
     CDS             1114..1524
                     /locus_tag="LNE23_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBK6931467.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="LNE23_001611"
     CDS             1517..2218
                     /locus_tag="LNE23_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBK6931468.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="LNE23_001612"
     CDS             2218..3804
                     /locus_tag="LNE23_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6931469.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="LNE23_001613"
     CDS             3823..4044
                     /locus_tag="LNE23_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBK6931470.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="LNE23_001614"
     CDS             4041..4802
                     /locus_tag="LNE23_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBK6931471.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="LNE23_001615"
     CDS             5182..5691
                     /locus_tag="LNE23_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK6931472.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="LNE23_001616"
     CDS             complement(5766..6305)
                     /locus_tag="LNE23_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBK6931473.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="LNE23_001617"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="LNE23_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBK6931474.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="LNE23_001618"
     CDS             7318..8169
                     /locus_tag="LNE23_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBK6931475.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="LNE23_001619"
     CDS             8197..8832
                     /locus_tag="LNE23_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBK6931476.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="LNE23_001620"
     CDS             8852..9625
                     /locus_tag="LNE23_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6931477.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="LNE23_001621"
     CDS             10033..10830
                     /locus_tag="LNE23_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBK6931478.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="LNE23_001622"
     CDS             10808..11221
                     /locus_tag="LNE23_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK6931479.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="LNE23_001623"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="LNE23_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK6931480.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="LNE23_001624"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="LNE23_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBK6931481.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="LNE23_001625"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="LNE23_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBK6931482.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="LNE23_001626"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="LNE23_001626"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBK6931483.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="LNE23_001627"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="LNE23_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBK6931484.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="LNE23_001628"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="LNE23_001628"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBK6931485.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="LNE23_001629"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="LNE23_001629"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBK6931486.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="LNE23_001630"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="LNE23_001630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBK6931487.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="LNE23_001631"
     CDS             21905..22483
                     /locus_tag="LNE23_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6931488.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="LNE23_001632"
     CDS             22535..23017
                     /locus_tag="LNE23_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBK6931489.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="LNE23_001633"
     CDS             23134..23508
                     /locus_tag="LNE23_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931490.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DAFCNS010000042        23653 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFCNS010000042 DAFCNS010000000
VERSION     DAFCNS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.042"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..506)
                     /locus_tag="LNE23_001634"
     CDS             complement(<1..506)
                     /locus_tag="LNE23_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6931491.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="LNE23_001635"
     CDS             779..3250
                     /locus_tag="LNE23_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6931492.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="LNE23_001636"
     CDS             complement(3353..5089)
                     /locus_tag="LNE23_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBK6931493.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="LNE23_001637"
     CDS             complement(5086..5790)
                     /locus_tag="LNE23_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6931494.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="LNE23_001638"
     CDS             5921..6424
                     /locus_tag="LNE23_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBK6931495.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="LNE23_001639"
     CDS             6384..6722
                     /locus_tag="LNE23_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBK6931496.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="LNE23_001640"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="LNE23_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBK6931497.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="LNE23_001641"
     CDS             8273..8851
                     /locus_tag="LNE23_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBK6931498.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="LNE23_001642"
     CDS             8855..9376
                     /locus_tag="LNE23_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK6931499.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="LNE23_001643"
     CDS             complement(9455..10009)
                     /locus_tag="LNE23_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK6931500.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10262..10537
                     /gene="rpsP"
                     /locus_tag="LNE23_001644"
     CDS             10262..10537
                     /gene="rpsP"
                     /locus_tag="LNE23_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBK6931501.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10549..10800
                     /locus_tag="LNE23_001645"
     CDS             10549..10800
                     /locus_tag="LNE23_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBK6931502.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10925..11716
                     /locus_tag="LNE23_001646"
     CDS             10925..11716
                     /locus_tag="LNE23_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931503.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11886..12407
                     /gene="rimM"
                     /locus_tag="LNE23_001647"
     CDS             11886..12407
                     /gene="rimM"
                     /locus_tag="LNE23_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBK6931504.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12397..13158
                     /gene="trmD"
                     /locus_tag="LNE23_001648"
     CDS             12397..13158
                     /gene="trmD"
                     /locus_tag="LNE23_001648"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBK6931505.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13294..13641
                     /gene="rplS"
                     /locus_tag="LNE23_001649"
     CDS             13294..13641
                     /gene="rplS"
                     /locus_tag="LNE23_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBK6931506.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14014..14676
                     /locus_tag="LNE23_001650"
     CDS             14014..14676
                     /locus_tag="LNE23_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931507.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14755..17982
                     /gene="fss3"
                     /locus_tag="LNE23_001651"
     CDS             14755..17982
                     /gene="fss3"
                     /locus_tag="LNE23_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBK6931508.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18096..18410
                     /locus_tag="LNE23_001652"
     CDS             18096..18410
                     /locus_tag="LNE23_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6931509.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18423..18797
                     /locus_tag="LNE23_001653"
     CDS             18423..18797
                     /locus_tag="LNE23_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6931510.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18807..19151
                     /locus_tag="LNE23_001654"
     CDS             18807..19151
                     /locus_tag="LNE23_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBK6931511.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19222..20574
                     /locus_tag="LNE23_001655"
     CDS             19222..20574
                     /locus_tag="LNE23_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK6931512.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20634..21776
                     /locus_tag="LNE23_001656"
     CDS             20634..21776
                     /locus_tag="LNE23_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBK6931513.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21801..22055
                     /locus_tag="LNE23_001657"
     CDS             21801..22055
                     /locus_tag="LNE23_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931514.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22311..23495
                     /locus_tag="LNE23_001658"
     CDS             22311..23495
                     /locus_tag="LNE23_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBK6931515.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23492..23629
                     /locus_tag="LNE23_001659"
     CDS             23492..23629
                     /locus_tag="LNE23_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBK6931516.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7832 a         4218 c         5073 g         6530 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10081 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10141 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10201 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10261 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10321 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10381 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10441 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10501 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10561 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10621 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10681 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10741 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10801 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10861 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    10921 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    10981 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11041 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11101 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11161 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11221 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11281 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11341 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11401 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11461 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11521 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11581 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11641 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11701 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11761 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11821 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    11881 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    11941 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12001 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12061 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12121 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12181 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12241 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12301 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12361 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12421 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12481 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12541 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12601 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12661 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12721 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12781 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12841 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    12901 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    12961 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13021 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13081 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13141 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13201 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13261 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13321 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13381 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13441 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13501 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13561 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13621 atcaaagaaa tcagaagata aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa
    13681 gtccttgtat atcaagggct ttttcttttt tctctatcac agttttaagc catttttggc
    13741 tgttttggac catttttcaa tcatattccc ttaaaaattg tcctagattg tccgacattg
    13801 actatcagtg tcgtcaaaat agggcaaaat ttagtagtca gcttgacgac aaatataata
    13861 cgaaaaatac caaaacattt accgaattta gcaacttttg aacccaagtt tctcaaaggg
    13921 tcagctatta gcaaaaaaga caaaacatgt acttttctac taaaaagttg atgtgatagg
    13981 cttctatcta aataactatt aggagttttt atcgtgaagc aaaatgatgg acgtatcatt
    14041 tggaaaaatc aaagtgagtt aaaactgatt ttaacaatta atgaattcat cgaaaaacac
    14101 ggaattacat ctagccgtca ataccagaaa aagctatcag aaaatccaaa ttctgcacca
    14161 agtatgtggt tcattaacaa aaaatatgga tcatgggaga atttattgat tagtatagga
    14221 cgagaaaata ctgggtatgg aaaatgggct agaatgtcag aacaagaatt attggaaata
    14281 gtagaagcct ttattaagtg cgagaagata acctctcaac gaatgtacga acaaaaatca
    14341 gtagggaaga atattccttc acttagtacc attaaaaaga tgttaggaga tatacgtcca
    14401 ctttttaaag aaaaaaatga tggatctcga tttactgatt tcgaactgct gttagaacta
    14461 aaaaatgaaa taatccgatt aaaactacag gatgatttat caatgacaaa gtttcgaaaa
    14521 ctagttcaat ccccaaaact accatcagtt gatacaatta tgaaaagaac gaacaaaaat
    14581 tgggaggaat tgatggcaga gattggattt gattatcgaa gaatcaaaat ttataagcag
    14641 agaaacaacc tatccaagac aaagaaaact aaatagtatc actctaaatc agacgttcaa
    14701 aattaaaatt tggacgtctt ttttattacc caaaaacgaa aggagattta ttttatgaaa
    14761 aagccaagat ttaagaattg gcgtttgctc gcaacactga gtctattatg ccaaacaatt
    14821 ggtggatcgc ttggcccaac gattgccttt gctgatgaaa ttactcatcc acaaacggta
    14881 actgtggaat tggatttggc tcaccagtat gctgtcgaag gtacatttag cgatggtcgt
    14941 cccatgtcag aagttaccgt tccgcactat gcagtttaca atggtgtgaa gcaagatatt
    15001 ttctgtattg agccaggggt gccgatttac aatgagttca ctcctggtta tgagaaaaat
    15061 ccattgcctg atatgtcgga gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt
    15121 acggatgtgg atacacatat tgtggcccag aagatgattt ggcaagaagt taatggctac
    15181 acgcttcact caattaaacg ttctgatggt agtgcagtaa atatcgcagc cattgagtca
    15241 aaaatcaatc aagccattgc ggattatcaa aagaaaccaa gcttccataa ctccacagtg
    15301 aaaaccgtgt taggtcaatc gactactgtg acagatacga acggcttgaa tttatcggag
    15361 tttgatgaag ttgtcgagaa tatcgcaaat attgattatc gtgtgaacgg caatcaacta
    15421 gtgattactc caaatgccaa ttcaaaagag agtggtgaat taaccctaaa gaagtcagct
    15481 aatacaggaa caccggtagc ttataaaatg gctggtcaac aaactttgat ggcgggggca
    15541 attgataagc ccaataccta cacaatcaaa attgatgtgg aaactgaggg ttctttgaag
    15601 attaaaaaag tcgataaaga aacaggaaat gttgtaccgg gaaccgtttt ccatttagac
    15661 tttggaaaaa cattacctgc aaaagacgtg accaccgata aagaaggcat tgcgacattg
    15721 gatgagattc ctcatggcgc gaaagtaacc attactgaaa aatcagtgcc agctccttat
    15781 acgattgaca ctacaccaat caccgcaacc attaaatctg gtgagactat ttctgtgact
    15841 tcaaaaaata ctcgcgaaaa aggtcaaatc attttgaata aaatgggggt ggaaacagga
    15901 actgatcttt ggaacgacaa atactcatta gccggaaata cgtttgccat tcgcaaagac
    15961 agtccaaccg gtgaaattgt ccaagaaatg accacggatg aaaaaggtca tgcggaaaca
    16021 ccaaaagaga ttgccaatgc gttggaattg ggaacctatt atgtgaccga aaccaaagct
    16081 agtaatggct ttgtgaatac ctttaaacca gtaaaagtcg aattgaacta tgtcaatcaa
    16141 accgtggctc ttgttaccag taacgtaaaa gggcaaaacc aagaaatcac tggtgaaacc
    16201 actttgacaa aagaagacaa agataccggt aatgaaactc aaggtaaagc tgagtttaaa
    16261 ggagctgaat atactctctt tactgcaaaa gatggtcaag cggtcaaatg gagtgaggct
    16321 tttaaaccag agttagtgaa gggaacgaaa gcttctgatg aaacggtgac tttgacttta
    16381 gatgaaaaga atcaagttgc tgttaaacac ttagcgatta atgagtatta ctggcaagaa
    16441 accaaagcac ctgaaggata taccttggat gaaacaaagt accctgtttc catcaaaaag
    16501 gtggatgata acgaaaaaaa tgccgtgatt acccgagatg ttacggcaaa agaacaagtc
    16561 attcgctttg gctttgattt ctttaaattt gctggatcag ctgctgggac tgccgaaact
    16621 ggcttcaatg atctgacctt taaagtatcg ccattggaag gaaccagtga gattacaggt
    16681 gccgaagatg aagcaattac agcttataac gagcaactag gttttgatgg ctatggcaag
    16741 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaggctcc agaaggattt
    16801 caaaaaatta cgccattaga aatccgttct acttttaaag aaaataaagc ggattacact
    16861 aagagtgaat acatttttac gattaccgaa aaaggacaaa aacaaccaat taaaacggtg
    16921 accgttcctt acgagaaact gacgaataaa gcattttctg ttagcttgaa tcgtttgatg
    16981 ctctatgatt tacctgaaga agaagatagc ttaacttctc ttgggacttg gaaagacgga
    17041 ggcaaagaat tgacagacct tgattctacc gagctagttg ataaattgag ctataacttg
    17101 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga agcgacaaaa
    17161 gctgcccaag aaaaagatga aaaagccaaa ccagtggtaa ttgccgaaac aaccgcaacg
    17221 ttggcgaata aagagaaaac aggaacttgg aaaattttgc ataaattaac cgcagaacaa
    17281 gttttggata aaaccatcgt cttattcaac tatgtgtatg aaaacaagaa accctatgaa
    17341 gcaggtgatg atccagtagc gaaagatgct agcttgaaca atcaagccca aactgtcaat
    17401 tgtacggtgg aacgccatgt ttccatccaa acaaaagccc acctagaaga tggttcgcaa
    17461 acctttactc atggggatgt ggtggatatg ttcgatgatg tgtcgattac ccatgatgta
    17521 ctggatggct caaaagaagc ttttgaaaca attctgtatg cattacttcc ggataatacg
    17581 accaaagaaa tttggaaatc tggcaaaatt gattatgaag tgaatgacaa agaattcact
    17641 aaaaccgtac ttgcggaaaa agtggacaca agtaaatatc cagaaggtac aagttttacc
    17701 ttcaaagaaa ttaactatgg caaagacgga aacatcaatg ggaaacacaa tgaggatcta
    17761 aaagaaaaaa ctcaaacttt aacacctaaa aaagttccga caacaccaag tgcaccagaa
    17821 caaccggaaa taccaactgt tccaagtgac tctcaagaat ctagtcctac agtaaagaca
    17881 ttcccacaaa caggggagaa aaattctaat gttctgctgt ttattgggtt taccttgatc
    17941 tttgcgactg ctgggtatta tttctggaat cgccgaaact aaggtgatgt gatgaaacaa
    18001 ctaaatagga aaaaccaacg tgcgcgaccg cctcctaaaa aaagtggtcg cgctattttt
    18061 gtatccaaaa ctaaaaatga aaagaggaaa agaagatgga attgaaattt gttgtgccag
    18121 atatggccga aacctttgga aagataaggt atgccggcga aggcgaagtt ttaactgaag
    18181 gatatgggcg gaatactaca gtaattggtc gtagttatca tttgtattcc agtaagcaac
    18241 gagccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgatcaag
    18301 atcaaccact aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    18361 gtgggtttac cgactacgtg ttgtatgtcg atgatttagt gaaagcatag gaggaaaaga
    18421 agatgagatt aacagaaggt attgttgtag attcaggact aacgtttggg aaattacgat
    18481 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacggtt agtaacgagg
    18541 taaaagaacg gacttataat ttgaaatcct ctgcgcaagg tcgaatgatc caagttagta
    18601 tcccagcaaa tgtaccctta cgcgagtttg cctatgacgc agaggtagag ttggtcaatc
    18661 caatcatgga tacggtcgcc aattatgttt tccgagaagg aactactgtc aattggttca
    18721 ttaaggccga tgatttagtc ttgaaacgac aaccagatca gggaaattct acaaaccaaa
    18781 atgaaggaaa gaaataggtg aagcgtatgg aaattaatgt tgaacatatt ttagattgtc
    18841 tcgatcaata tggaaaaggt gagctaacgg aggaacaact cactaaagca ttaacctatg
    18901 atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgtcgaag
    18961 aagattttga tgtactgaat tggttagggg aagaagagtc cttctttatg gttatcgaag
    19021 taaatgaaag tctatgtcga gaagccgaaa ccgtattgga agaaattggt gtggaaatgc
    19081 cggatgccat tgaagttttt ttaaagcaac tagtggaaac gaaacaactc cctatagcag
    19141 taaatgacta gcataactgc ttgaaattgg atcaatgaaa tcctatgaag cttttttcga
    19201 ttggaggtgg aaaccataaa aatgtacaaa gggcatcgta tacgggcagg agatcagcac
    19261 ttggtttatc actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc
    19321 ttttattttc aagagcttag acaatttgat atttctaagc tatcactttc tactatagaa
    19381 atcgttcgtt ctataaaaga tttaatctat ttgctaggaa gtctcgttct ttcaggaagc
    19441 acgatgttgc tttatataca cttttttcag gatcattggc gcagtttgtg gcatcgacaa
    19501 aagctagccc ggatgattct agaaaatcat tggtacgaag taaaacaaac tcagagtgaa
    19561 ggatttttca aagacctcaa tagtagtcga accaaagaga caatcagtta cttccccaaa
    19621 atctattacc gtatgaagga tggtttactt tctattcgcg ttcaaatttc actgggaaaa
    19681 tatcaagatc agcttttaaa gctggaaaag aagttagaaa gtggtctata ttgtgaacta
    19741 gtagaaaagg aactcaaaga ctcctatgtg gagtacactt tactgtatga tataattgcc
    19801 aatcgaattg ggatagacga agtagtggca gaaagtgggg ctttgcgatt gatgaaaaat
    19861 caagtatggg cttatgattc tttacctcat atgttaatcg ctggcggcac aggtggtggg
    19921 aagacttact ttttactcac tatcatcgag gctttattaa aatctgatgc cgagcttttt
    19981 gttcttgatc ccaaaaatgc ggacttagcc gatttaggta cggtaatgcc tcatgtctat
    20041 tcccaaaagg aggaaatttc tgcttgtgtg gaagatttct atgagcgcat gatgactcgt
    20101 agcaaggcaa tgaaagaaat gcccaactac aaaacaggag agaattatgc gtttcttgga
    20161 cttccaccaa actttttaat ctttgatgaa tacgtggttt atatggaaat gttaacgacg
    20221 aaggaaagtg cggtgatttt gaataagctc aaacaaatcg tgatgttagg tcgtcaatct
    20281 ggtttctttc tgattctagc ttgtcaaaga ccggatgcga aatatttagg agacgggatt
    20341 cgcgatcaat ttaatttccg ggtggctttg ggtcgtatga gtgaactggg atattccatg
    20401 atgtttggcg aggttgataa aaaattcttt atgaagcgta tcaaaggtcg aggctatgta
    20461 gatacgggag ggagtgtgat tagtgagttt tatacgccac ttgtaccaaa aggatatgat
    20521 ttcttaaggg aaattagcga cattggtgga ttaaacgtta atacggaagg ataaaataat
    20581 agtatgtagc ctaggagtgt caagaatcct gggctttttt tacgaggtga tttatgacat
    20641 ttttagattt attcgctggg attggtggct tttgcttagg tatggaacaa gcgggtcacc
    20701 agtgtatcgg cttttgtgaa attgatgatt ttgctcgcca gagttataag gcaatccatg
    20761 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagacgag tttattcaat
    20821 ctctcggacc agtggatatc ctttgcggtg gatttccgtg ccaagctttt tcaattgcgg
    20881 gaaagcggca aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg
    20941 ccgctattct ccaacctaag tttttattcc ttgagaacgt ccggggatta ctcaatcacg
    21001 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgatgtgg
    21061 aatggcaagt gcttaactca aaggcctacg ttccccaaaa ccgtgagcgg atcttcctta
    21121 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga tcgtctccaa
    21181 catctgatca aaacatcaga aacttattaa atatcaaccc ttccaatcga ggaatggggg
    21241 gacaagtcta tgggtcagat gacgtagcgc ctaccttgac cggtgatgaa ggaatcaaaa
    21301 tcgcattgcc ggtagatgat ggaatttctg tagcgggtat gttactggga aattttgaac
    21361 aaggcaatcg ggtttatgag ataactggaa cagcgcctac tttgtcaacg aaacaaggcg
    21421 gaacgaagat catgcttcgg aaaaacgaaa cagaatatca agaggtgaag ccagggaaca
    21481 gtgtgaacct ggcttttcct aattccacaa gtcgtcgagg ccgtctggga aaacaaagtg
    21541 tgcatacctt attaacagga gaccaacaag cagtcgtcac ggatcagtat caaattcgaa
    21601 agttaacacc tagggagtgc tggcggcttc agggctttcc ggactgggct tttgatcgag
    21661 cttctcaagt caattcagat agtcagttgt ataagcaggc tggaaattct gtaacagttc
    21721 cggtcatttt tgatattgct agaagattaa aggaggtaaa aacagatgat ttatgatgag
    21781 ttgattgggg aatctactgg gtgattggca agattcagtc agatccggaa ttggaagaag
    21841 aactgtaccg gctcaatttt gatattcgca aaaacggggt gaaagtccct gatgatccgt
    21901 atgtaattga tgaggaaacg gatgctcgaa tggagctgaa tcaagtcatc agtgaattgg
    21961 aacgaattgc ggactttgct aaagaacctg atatccgcca atacttgttt gagataaaag
    22021 cagagttgga aattgagggt attaccgcgg agtaacggcc aaataaacca ctgataggca
    22081 ctgaccgcgg gggatttgcg aagcagaaat cctccgtggt gctgtgcctg tttatggacg
    22141 gcgttagccg gccatgatgt ctcacccccc gtatctaaca ggggggtaca aaaacacaaa
    22201 aaataagcaa gcaacgataa ggaatggctg tcctatcaag gataataagc aatttgtgta
    22261 gatgttaact gatttgaaaa agttaacacc ttaaatttgg aggtgagaac ttggcacaaa
    22321 ggaatttaga ttatcggcta ttaaaagacc ggcgtaatga atatggcgtt tcacaaaata
    22381 aattagctac gacttgtgga cttagtcgcc cgtacttaaa tcagatagaa aatggtggcg
    22441 taaccgcatc gacaaaaacc atgaggaaaa tttttgacca actggaaagt ttcaatcccg
    22501 agttaccttt aacgttactg tttgactatg taaggattcg ctttcccaca acggatgcga
    22561 ggaaaatcat tcaagagatt ctccatttga aatttgatta tatgctccac gaagattacg
    22621 ccttttactc ctatcaagaa caatatgtta tgggagatat tgtggtgatg ttgtcacatg
    22681 aagaagataa aggcgtcctt ttagaattaa agggtcgtgg ttgtcggcag tttgaaactt
    22741 ttttactcgc ccaaaagcgt agctggtatg actttttcga agattgtcta aaagccggtg
    22801 gcgtgatgaa acgcttagac ctggcaatca atgatcgagt tggcctgtta gatattcctg
    22861 atttaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc ttcaaaagct
    22921 atcgttccgg ggaactgttg aaacctgatg ataaagatgg tatgggcaat actttgtata
    22981 ttggcagtct taagagtgag gtctatttct gtttgtacga aaaagactat gagcaatata
    23041 tcaagttagg gattccttta gaccagaccg aaacgaaaaa tcgttttgaa atccgactga
    23101 aaaatgatcg agcttatcat gcaattcaag atttgttaaa aggtcgcagt attgaaagta
    23161 ccacattttc cattattaac cggtacttgc gatttgcgga taaggtggaa ggcaaaaggc
    23221 gaactaactg gcccttgaat gaacaatggg ggcgcttcat tggacggaat cagaaggaaa
    23281 ttcaattaac ttctgaaccg aagccttata ccattgaacg aacgttgaac tggcttggac
    23341 gacaagtcgc ccctacgtgg aagatggcca aggagttgga tcggttgaat cagacaacgt
    23401 acattcagga tatggtaaat aatactcgac tatcagaccg gcacaaaaag attttggagc
    23461 aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtga tggaaattgt
    23521 gaaagatatt ttattggtgt ttggtggcag tatgcttggt gttactgtga tgtgcttgat
    23581 gcatgcaagt gcagcggcgg accgagcaat ggaaaagaaa ggaaagtgat gaaatgaatt
    23641 tcggtcaaaa ttt
//
LOCUS       DAFCNS010000043        23218 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFCNS010000043 DAFCNS010000000
VERSION     DAFCNS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.043"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(83..283)
                     /locus_tag="LNE23_001660"
     CDS             complement(83..283)
                     /locus_tag="LNE23_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6931517.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(736..1056)
                     /locus_tag="LNE23_001661"
     CDS             complement(736..1056)
                     /locus_tag="LNE23_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931518.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1559..2677)
                     /locus_tag="LNE23_001662"
     CDS             complement(1559..2677)
                     /locus_tag="LNE23_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931519.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            3148..4455
                     /locus_tag="LNE23_001663"
     CDS             3148..4455
                     /locus_tag="LNE23_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBK6931520.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            4576..5775
                     /locus_tag="LNE23_001664"
     CDS             4576..5775
                     /locus_tag="LNE23_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK6931521.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5802..7070)
                     /locus_tag="LNE23_001665"
     CDS             complement(5802..7070)
                     /locus_tag="LNE23_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBK6931522.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(7240..7881)
                     /locus_tag="LNE23_001666"
     CDS             complement(7240..7881)
                     /locus_tag="LNE23_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBK6931523.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(8290..8793)
                     /locus_tag="LNE23_001667"
     CDS             complement(8290..8793)
                     /locus_tag="LNE23_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6931524.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8916..9680)
                     /locus_tag="LNE23_001668"
     CDS             complement(8916..9680)
                     /locus_tag="LNE23_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBK6931525.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9788..10063
                     /locus_tag="LNE23_001669"
     CDS             9788..10063
                     /locus_tag="LNE23_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBK6931526.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10623..11573
                     /gene="yidC"
                     /locus_tag="LNE23_001670"
     CDS             10623..11573
                     /gene="yidC"
                     /locus_tag="LNE23_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK6931527.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(11619..12020)
                     /locus_tag="LNE23_001671"
     CDS             complement(11619..12020)
                     /locus_tag="LNE23_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK6931528.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(12034..12843)
                     /locus_tag="LNE23_001672"
     CDS             complement(12034..12843)
                     /locus_tag="LNE23_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6931529.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFVALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(12830..13720)
                     /locus_tag="LNE23_001673"
     CDS             complement(12830..13720)
                     /locus_tag="LNE23_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6931530.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(13733..14212)
                     /locus_tag="LNE23_001674"
     CDS             complement(13733..14212)
                     /locus_tag="LNE23_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6931531.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            complement(14388..15329)
                     /locus_tag="LNE23_001675"
     CDS             complement(14388..15329)
                     /locus_tag="LNE23_001675"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HBK6931532.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(15326..16321)
                     /gene="lacD"
                     /locus_tag="LNE23_001676"
     CDS             complement(15326..16321)
                     /gene="lacD"
                     /locus_tag="LNE23_001676"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBK6931533.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(16314..17510)
                     /locus_tag="LNE23_001677"
     CDS             complement(16314..17510)
                     /locus_tag="LNE23_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6931534.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDKTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(17528..18256)
                     /locus_tag="LNE23_001678"
     CDS             complement(17528..18256)
                     /locus_tag="LNE23_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6931535.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(18697..19671)
                     /gene="lacD"
                     /locus_tag="LNE23_001679"
     CDS             complement(18697..19671)
                     /gene="lacD"
                     /locus_tag="LNE23_001679"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBK6931536.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(19668..20126)
                     /locus_tag="LNE23_001680"
     CDS             complement(19668..20126)
                     /locus_tag="LNE23_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6931537.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(20123..21586)
                     /locus_tag="LNE23_001681"
     CDS             complement(20123..21586)
                     /locus_tag="LNE23_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6931538.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(21568..22500)
                     /gene="pfkB"
                     /locus_tag="LNE23_001682"
     CDS             complement(21568..22500)
                     /gene="pfkB"
                     /locus_tag="LNE23_001682"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK6931539.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            22642..>23218
                     /locus_tag="LNE23_001683"
     CDS             22642..>23218
                     /locus_tag="LNE23_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6931540.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         6976 a         4972 c         3899 g         7371 t
ORIGIN      
        1 actctcatcg ctttttaaag aaaatagaca aaaacgccca gacacgcaaa tgtctgggcg
       61 tttatttttt agtataggtt aattaagctt tagtaacgtt tgttgcttga ggtccgcgtt
      121 ggccttcttc aacatcgaaa gttactgctt ggccttcttc taaagttttg aatccgtcgc
      181 cttggatagc tgagaaatga acgaatacat cgtttccgtt ttctgcagtg atgaatccaa
      241 atcctttttc tgagttaaac cattttactg tacctgtttc cataataaaa atcctccttg
      301 tgtatcaaca catattttgc aattacttga atggtgtata tctggaagat gtttatttga
      361 aacaagctac cacagattac cgaacaaaaa ctactcctat agtatatcat accgtttaca
      421 aaaaagctag agtttttcga ctttttttat taaaaaaagt tttctcagcg attacataaa
      481 gtgaaaaacc gaactttttt ttaaaataaa cgacatattg ttcagttggt gtttttttgc
      541 tctgatgttc ttttttaaac taaaaaaatt attctttctg actacttatc ggttctgttt
      601 caacggtcga ggctggggaa gattctttag taggtctgat gttctcatgg tatttctgat
      661 agcctatgac agttaccaga agaacaaggc aaaccaatac gaccaacgag attagttttt
      721 cttttttgct catatctata cctcataaat aacaggatga tgtccatcat atttctgagt
      781 atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac tttcataatc
      841 acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt cttctgtttc
      901 aggaatcgca gaatcaagat ctggtgtctg tacataaaca tttttgtaca gacgtgtgcc
      961 tgtaatagga aaccgctgtg tttcttcagc aaaaactggc tgacttgtta aacttaggga
     1021 tagagacagc aaacaccata acagtctttt tttcataaag acctccttat cgataagttt
     1081 ttcccaatta aaatcatatc tcaagaacgc ccgttattca cattttgttc ataaatgatg
     1141 ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc ttttttaaac
     1201 ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat ttgagaaata
     1261 atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa cctcttcctt
     1321 aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa aatatgaaga
     1381 gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac aacctctcat
     1441 acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggcaaa atatgtgaaa
     1501 ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac aacctctttt
     1561 aataacgaag aatcgctgtc agactctttt catctggcgc atctttttga tccaaaataa
     1621 acacttttcc gccattaatg atcacatcat ctgctaatgc attcaacact tgacgccgat
     1681 catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta gcgataaata
     1741 aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga tcgatgaatt
     1801 tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca cttgacagct
     1861 tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca gaaatcgcaa
     1921 cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga ttttctggta
     1981 gagcaaataa ataaatcggc cattgttctg gattttcgaa agaatccttc aagtacgtat
     2041 caactgcttg gtaatagttg acccaatcga tctctacttc ttcatctttt ggatttacgc
     2101 catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag ttcaaaccgc
     2161 cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct tctggtagag
     2221 atacttcttt tacttgtttg ttgctgactt catacaattt catggagtcg cggttcaatc
     2281 ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt aaataaggaa
     2341 gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata taggtttggt
     2401 cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa ggatctccta
     2461 acaaattttc aattttttct tggaacgggg accagtcatg ttcggaatat tttttctcaa
     2521 aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt tccacatctt
     2581 gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg ccttctgaag
     2641 tcaattgtga caataattct ttatcctttt ttgccataaa cgtacccctc ctaaaaaaat
     2701 cattcctttg tttctatcta aagtctacca caacactatt tttccataca ataaaacgta
     2761 tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta agttatcttc
     2821 ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat gctgataaac
     2881 taagaaaaag aataaaagag gtggatctta tgcatttgat tatgattttc agtttattaa
     2941 gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa acacagccta
     3001 ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat aacgtgcgtt cttatcctaa
     3061 agttttttca gaaacattga aactctctcc cctctctgat aaaattttaa tttgtagcga
     3121 aacaaattaa ttacaaggag atcggatatg tccataaaaa aaacacgtct agtcctatta
     3181 ttggctagtc tcattctttg ccttgcttct tttctaccag ctgttgctgt gcaagctgaa
     3241 gatactttca aagtaaatgc aaaagcagcc tttgctgtag atgcgcaaag cggtaagatt
     3301 ctttatgatc aagatggaga aaaaaccatg gggatcgctt caatcacaaa aatcatcggt
     3361 ctttacatcg tccttgatca agtaaaagaa gggaaactat catgggatga taaagtttca
     3421 atttcagatt atgcagaaaa tttgagtgtc accccagatt tatcaaatgt tcctttgcat
     3481 aaagaaaaca cctacaccgt taaagaatta ttcgactctg caatcatcca gtcagccaac
     3541 gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg aagcaaaatt cactgaacga
     3601 atgaaagaac aattgaaaga ttggggtatc aaagatgcaa cgatcgttaa tgcatcagga
     3661 ctgaataact cttatcttgg cgaaaaccga ccagaaggta ctggagaaaa cgacgaaaat
     3721 caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt tgatcttgga ttttccagaa
     3781 atactggatg tatctagcac tacgacacaa atgtttggcg aaaacacgca atcaccagta
     3841 gaaatggtca actggaactg gatgctccca ggattcgtga attataaaga aggcgtggac
     3901 ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt tcgtcggtac gatcactaaa
     3961 aacgatcaac gaatcatcac tgtcgtattg aatgcagacg gccatgcgga aaacccaagt
     4021 gcccgtttca atgaaacagc tcgcttaatg gattattgct acgacaattg gtcagaaaaa
     4081 gagcttggaa aagccaatgc aagtatccct agtctaaaaa ccattgatgt aaaagacgga
     4141 aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa aagtatgggt acggaacgat
     4201 atggatactg gaaaattaac aatcacacca actcttgaca aaaaacaagt cactgacaat
     4261 gagatccaag cacctatgga taaaggaaca aaaatcggta ccgcaacgat caccttggca
     4321 gaagataaat taggttatct tgaagacaac aacgcgccat ccacagaaat catcacggat
     4381 aaaaccgtag aaaaagcaaa tattttcgtt attggctggc gacgcgtcgc agaatttttc
     4441 ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt tccatgtgga agatgttttt
     4501 ttgctttttc tagtttaaat aacagctttt ttcatctatt ttcgttatac tgtataagaa
     4561 aaaggagttg aatttatgaa tctaaccaga gatctgattt ttttggttct ctatcttatt
     4621 ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg atccttcctt gttcttttac
     4681 agcagtacat ggacgtttgc ggtaatcttg acttattttg tcgtgttgga aattttttat
     4741 tcgacctttt attttttgat tcccttagtg tttttcagta tctttgcctt ttcctatttt
     4801 tcagaaaagc gaaggctgtt gaatggtctt ttattcaata ttttcctcat tagttttggt
     4861 atctacttgt ttgtcttgct atacgaaaca caaaatattt ttcttggcgg actgattgcc
     4921 ttgatcacga tcccgttact gcttgtcttt ctattcggca tttatggatt gatcgttttt
     4981 cttttttgga acggtgtcac agttttacgt agagagtcgc attcattagc taatctttta
     5041 acgttgatac tagccatttt tcttacattg tttcttgttt ttgacttttt cttgttaaaa
     5101 tatttaccac aatggataaa tgctttgttt ttctgcatcc cattgatctt gatctatcta
     5161 tttatcgtat tctacaactt tttgactgtc tcattcttgt atcagttcaa ccgccctcga
     5221 tacaaccaag acttcatcgt tgtgttaggc gccgggttga tcaatggaga aacagtttct
     5281 cctcttcttg ccaagcgaat caataaggcg attgcttttt attgggcaca aagccgtgcg
     5341 acattaaatc cgcctatcct attaatgtcg ggtggacagg gagcagacga aaaagtacca
     5401 gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa ttcctgaaag agatattctt
     5461 gtcgaaacca attcgacaac cactttggag aatatgcgct attcaaagga aatcatggat
     5521 cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca acaattacca cattttcaga
     5581 gctggtctct atgcacgaca agcgaaactg aaggcaaatg gcattggggc aaaaacagct
     5641 ttctattact tgcctaacgc gtttttaaga gaatacatcg ccatcttgat gatgaacaaa
     5701 aaaagacata tgatcatttg cggattgctt ttcgcaggtt ccgcattctt atcactgatc
     5761 actttacttt tctaaaaaaa caaggtgtga caaaacatat gtcacacctt gtttttttct
     5821 tttattctct tgacgattgg caagaatagt attccttttt tgacggctac ttgctgtgca
     5881 gaataaatac cgctgtttcc tgaacacata aagctgatca agcagatcat aaagaagtaa
     5941 gcagcagctt gtgaaccaaa taattcgatt cccatcacaa aacaggcaat cggtgtattt
     6001 gtcgcaccag aaaatacccc gataaaacct aatccagcaa gaaaaggaac agaaacatgg
     6061 agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct caaatagtgg tgtcacttct
     6121 cccccttggt accccgcacc taaagacaag accgtgaaga ataatttccc aataaaatca
     6181 taagcatgtg cctgtccatt aaatgattcc tgcagtaacg gaagactcaa cccaaggtaa
     6241 cgttgtgttt gcaaaatcaa cgctgcggct acaactactg ctccacctat cgtatttcgc
     6301 caaacaggat gaggaatcca ttgcgcatac cgcgctttta aaaagacgat cgaacggctg
     6361 aatacccaac caatcaaacc aaaagcaatc ccagcaacaa aaactttgaa gaacagcaac
     6421 atactccatt caggaatcac acccatttgg taatgtagat gagaaacacc atagctttct
     6481 gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa atattgcttc tgttcggact
     6541 ttaccgattg ccaacacttc taatccaaat aatgttccag ctaaaggtgt gccaaataca
     6601 gaactaaaac ccgcactgat tccactgata accaaaatct cccgttctgc tttatccaat
     6661 cggaataaat gcgctatatt atcagctaat gctcccccca tttgaactgc tgttccttca
     6721 cgaccaacag atcccccaaa taaatgggta gtgatggtcc caaacaaggt caatggaatg
     6781 agccgcaaag ggatcttctc ttctcctcca ttcccttgat cgatcactaa gttattccca
     6841 cgactagaaa gaccgccatg atacgcataa agataagcaa aaagcgcgcc gctgactggc
     6901 aatagataaa gtagccaagg atgtgctagt cggatatctg tcacgaacgt caggctattt
     6961 aagaaaaaag cagacaagct ccccatgaat actccgagaa tcgctgtgac caccagccac
     7021 ttcaacatgt acatcaaaat acgcactgga ctcggtaatt gtttttccat aaaaaattcc
     7081 tcctcaaata aaaaaacgcc tactaaagaa aggggctggc cccagacttt agtaggcgtc
     7141 attagctatc ttacgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     7201 agatagcata tttttctgta aaaaacaata aaattatcgt tatttcttta cacgttcagc
     7261 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattgatcac tatcatgaca
     7321 aatataattc ccctttttcg tatacgtgcc atcagaaccg gctttcgttg tagaaagaaa
     7381 gagcgagaca ttcgatgttt tctgacagat cgcacagatt cccttattaa tcgttggtga
     7441 taaagtaccg ctgatccctt ttaattttcc atcttcgtaa taaaccatga attttttttg
     7501 cgtccctgga tcattccaac cgatgtacgt atgtgcttgc aagtcgattt cattccaagc
     7561 gggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctcggctg
     7621 cataaacggc tggacgattt gcttcagctc ctccaatgct cgttctgctt gcgtttttgt
     7681 cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt tctgatggtt cgtaacctaa
     7741 tacatcgcga atcttttcag ctgctagagc ctgaaccgtt tgtaccgttt ttgagtcatt
     7801 gacagatgag tacgcattta ataactgctc tacttctctt tttataaaaa aatattgata
     7861 tggtttcatt gtttgttcca tgattttaaa gtcctttctt ctaaccaaag tgcatacttt
     7921 gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga agatcccgga ctcttttaaa
     7981 taagatacgt tttgcatctt atcatcaccc cctttaatgt tcattcttct atgtacatga
     8041 ttcattcgat aaataaaagt aaaccgtatc ttgagcagat agtcaactgc aaacagaaaa
     8101 aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa acaaacggtg ttccagaagt
     8161 agcttcttca aaaaataagc cggaatttca caaaaatttg agaagcaatt ttcgtaaatt
     8221 ccttcttatt tcttgaagct gacccacttc tgtcacaacc tcaatatggt ctattattgg
     8281 ttttacctgt taaatcgagg attcttttcc gaagtaacgt tgccatctct tacgcatact
     8341 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     8401 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     8461 atctcgctca agatccgaca aatcggtgat cttttctgca ttctttacag cgattcttgg
     8521 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     8581 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     8641 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     8701 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     8761 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     8821 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     8881 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     8941 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     9001 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     9061 attgcgaagt tatctgattt ttctactttg tctaaggcgt gtgcttcttc atttaattct
     9121 gtgccataca ctgtaaaccc tttgtctttc attaaaggaa tcacttctgt caaatctttt
     9181 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     9241 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     9301 atcatcgtcc ctacatttcc aggatcttgt acattatcta ataacagcca tcctcctgaa
     9361 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     9421 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     9481 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     9541 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     9601 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     9661 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     9721 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     9781 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     9841 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     9901 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     9961 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
    10021 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
    10081 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
    10141 ggacttaatg ccgtagatgc aagcttttga acaccgtcaa taaggttgtg agaaagtcgc
    10201 ttagttttga gtagtaagga gaattttgtg aaaatggctt tccacatttt tgcataaaat
    10261 ttgacttaat accgagaaac tgacttttga acaccgtcga taaggttgtt tttgagacgc
    10321 tttgcctcaa gtattaaaga taggtagtaa aaaagctgat ctataacttg caataaattt
    10381 gcttaaccca tattttccag acagaacaaa aaagttttta gttttttaaa taagaaagaa
    10441 acacttggaa tcactctttt ctttccaagt atttctttct tgattttttg tctttttttc
    10501 aaaatttttc gctacgtaaa agaacgaata tttatatgaa atttctatga agttgattga
    10561 attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt
    10621 ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt
    10681 tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcggga atcgtttatc
    10741 gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgagaat ttcaattggt
    10801 cttatggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc ttgccactag
    10861 gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac
    10921 aagttgaagc agcccaacaa aaattgaaag ctgcaacaac acgtgaagaa caaatggcag
    10981 cacaagcaga aatgcagcaa gtataccgcg aaaacggatt gagtatgacc ggcggaatcg
    11041 gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtacttt acggcacgtt
    11101 atacagaagg tattcgggaa tcatctttct acggcatcga tttaggctca ccaagtatgg
    11161 tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg atacatctca acgatcggta
    11221 ttcctgaaga acagaaaaag acgatgcgta cgatgctaat cgtctctcct gctatgatcg
    11281 tcttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt
    11341 tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag
    11401 tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt cactccaaga aaagacgtga
    11461 cagaatcagc aaagccagct aacaacaaac aagcaacaaa acaaatcaaa caaccaaaaa
    11521 acagtacaaa caacaaaggc cgcaacgcag gaaagcagca gaagcggaaa taaaaacata
    11581 ggacaaaaaa gatgagagct aagccctcat cttttttgtt actcttcctc ttctgctatt
    11641 ccattaatta attcgttgac agagacaatg ctttcacatc ctgcacgaac aaactcgggc
    11701 tctgaaccaa tcatttgtga gttgataaat tcaatgacca tcggaagatt catcccagca
    11761 taaagagtaa tctgttgtcc ttccattatt agtttagaaa tgatatttgc aggtgtacca
    11821 cctaaaagat cgcatagtac cacaaaatca tttaacgaat ctgtgacagt gagaaacttt
    11881 tgtttgtatg tttcagtgcc atcttctggt aataaaggta acgtgtagat atcctcttga
    11941 ggccccatga tcatttctgt actttttttc aattcctcac aaaattttcc gtgactaatc
    12001 aataccaatt gtctactcat ttttcgctct cccttatttc gaaatcacac ccaatgcaga
    12061 taaagcaaca cacacaatta atacgataaa aatcgctcga gtaggagtca tgtttttctt
    12121 tcctaataac caataaacac caccgacgat cccagcaggt aaaattttag gtaagatcat
    12181 atcgatatag ttttgcatat tcaatgtcac atcgccaatc gttggtgccc atgaaaattt
    12241 aacattgatc attgtcgcaa ctaatgcccc taccatgaat acccctaata aagtggctgc
    12301 gtccgttaat gcagttaagc gagattgcat agtagtaact aaggaaattc cttctttgta
    12361 tgcaaattca agttgcttcc aacgaaaaac catgacaacg atctgagcaa aaatccatat
    12421 aaacacacct atcggatttc catcagttgc catattcgcc gccaaagccc caaaaatcgt
    12481 tggaatcaaa gcaacaaata cggaatctcc aatcgctgca aaggaaccca tcaatccagc
    12541 tttcagcccc gatacggttt cctttgaagc aattccctct ttttcttcca ttgcaagatc
    12601 aatcccagta atgattgtat taaaaaaatt agaagtatta aaaaattgcg tatgtgtttt
    12661 catcatctct tttaattctg gtgtattgtc tccatatatt tttcttagtt gtggcaagat
    12721 agtatataaa tagccagaac cctgcattct ctcatagttc caacctaatt gaaaagtgaa
    12781 caaacttctt cgattgattt gcttaaaatc ttcttttgtt aatttgtaat tagatttcgt
    12841 catcttcgat ttccccctca acagaatgtg atggctcact agctaatggt tgatggtctt
    12901 tgttttgatc attcttataa ctgatcattg ctagtgctag accaatcaat gctacagcaa
    12961 gcatcggcag cgaattgaaa gttgccggaa gatctttaac aacgcttgcc acacttgttc
    13021 ctaataattg aacattagag aataacgttg ctaataatgc cgtgatcgta aatcctaaaa
    13081 taagataaga tagatgtttt tttactggta aatagcgcag aagaatggca aacccaacag
    13141 ctggtaaaac agctcctgct actgaaagtc catcgcctag ccattttaga ttcccgttta
    13201 aaacagaaac aacattttca actaatccac caccaaatgc taaagctaaa aatacaggaa
    13261 tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa attgcgttcg attgcttgat
    13321 aattcatgtc ttctacgtga cggtcaatac gatgggcaaa gtatgtgtta gcgaatctag
    13381 ctaaaatatc caattggatc attaagcttg ctacaggcac tgcaatagca gcaatcgctt
    13441 gttctggatt cattccaaga gaaatcgaga aggcagttgc taaaatcgtc ccagagttgg
    13501 catctatttt tgatgcgcct ccaaacgtcc ccactcctaa aactgtcaac tgcatactgc
    13561 caccaataat caaacctgcg tttaaatcac ccattactaa acctgcatat aaccctgcaa
    13621 acactggcgc actcattgaa gaatagatct gtaattcatc taaaatttga taacctgcat
    13681 ataatgttaa taataagatc tgccaccatt gaatatccat ggatcattcc tcctatttga
    13741 ttaaaagatt cataaagtct tctgctttat cgtttggtac catttgagca attaaatgta
    13801 taccaagctg atctaatttt ttaaatactt cgatatctgc atctaccaca ttgattgatt
    13861 ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc cacatttact tctttgatag
    13921 gaactccttt ttctactaaa gataagattc gatctggctt ttttgctacg atcaatactc
    13981 gttgagagtc atatttgcca gctaaaatat ttgttgctgc tttttcgatt ggcaaaatgc
    14041 ttaattttac ccctgctgga gtcgctaact tcaatccact tttttcaatt gtgtttgtgg
    14101 ctacctcatc atccacgacc atgattcgac taatattcag tttcgttgtc caaagatttg
    14161 ctacttgtcc atgtatcaaa cgtccatcga ttcttacacc aataattgcc atcatgcatt
    14221 ctccttatct attttttcta tttattgttc aattaaaact gtgacatcat caaatatccc
    14281 ttctgtttca aagataacgt tttcattttt tctttcttat atttcatttt taagctaaga
    14341 acaaattcct tcccaactaa agaaaacgct ttcttttata tttaagttca tttgattttc
    14401 ttaacttgaa tgtgtttcat caacatcgta actttcatag ggtcgatcca gccagttttc
    14461 tcttgttgcg cgtttgccat accacaagcc atagaaaatg cgagaatctc ttcgattgat
    14521 tgcctttttg ataacgcatg ggccgatcct gcaagggtcg cgtcacctga accaacagga
    14581 ttaacaactg atattttagg aataatgact tcgtaaaaac actgagcaac ttttgccaca
    14641 gcacctctct ctcctaaaga aacaacaacc catggcactc cttcaaatat tggatgagct
    14701 aataaggact gaattttttc taattgaatc gaagaacaga tacgttggcc aatcagttct
    14761 cctaattctt ccagattggg cttgataagt agtggcttct ccttaccttc caatacttca
    14821 agcaaaaatt taccagaagt atctagtaat atgtctttcc cttttttatc agctaaagca
    14881 accaattgct tatagtaatc aagagggcat ccttggggta aactacctga gatcgtcata
    14941 atatctgata tttcgaggag ctgctcgaaa tgatcggtaa atgtctgttg ttctttcttt
    15001 gttatgaagg ggcctcgttc caaaagctct gtttgtctcg aactatgcag gatagcaata
    15061 gaatcccttg tctctccttt aattggaaca aactgatgca tgatccctaa ctgatctagc
    15121 tggttcttta gatattcacc gtgctggcca cctattaaac cagtagctat cacctcttct
    15181 cctaataaat ttagaaccct cgccacattc actccttttc ctcccgctgt tttattgact
    15241 gtttcaactc gattgacttt gtcgaggttt aattcttcaa ttgtatacgc gatatcgatg
    15301 gaaggattca aggtaactgt cgtaatcatt tcatcttcac ctttttgctt tttgttttat
    15361 tgaaccaagg ggtagcggtt tcatttatta ctttatttaa agtctcgatg ttttctctcc
    15421 catttgttct taaccaatga atagctccgt cttttccatt ttcgacgtat gctttgaccc
    15481 catctttcca tgtcgcacgt ccacacaata caccattgaa ggtggaaccc gattcatgag
    15541 caaaaatcag tgtttgttga aataattctg ttgaaacacc cccactcaaa aaaatgaatg
    15601 gtaatggagt cgcttcactt tgctccttaa agaaggcagc agcctcttct ttgctgtgaa
    15661 taaattcacc tgtggaatag ccattcacat aattcatatt gactggtact tctactttca
    15721 acacatcaac tttatatttt ggatcagaaa attctttcat catctcaata actttgtggg
    15781 gcttgacttt cgcatactct ggagaagaac tatcgcttat ttgtgcgtca taagaaacta
    15841 actccaaata gaagggacga ttttctcctt cgcactcact acctaaacgt tcgatgaaca
    15901 catgcttcaa atgattgatt tcgggtgctt catcaacatc gtagtaaagt aagaatttta
    15961 ctgcatccgc ttgctgttct ttcaatctga gagaagacca atccgctaat aaatcaggta
    16021 gacgaccagg cgtagttgcg tcatagccgg ttttctcata agctaacaat aaccccgtct
    16081 cttttgctct gacttttgct gctggcaatc catattccgg atccaataaa atagcagaag
    16141 catagggtgt cagctcagct gatactagtt ctttgaattc actgatttgg tcttcacttg
    16201 gctcttttcc caacgatttg atcatttttt ttagagcacc acgctgatcg atcgctaatg
    16261 cactaataat cccatctttt gttgatagtc tctccattgc cctatttttt tcttcattca
    16321 tctgtcattt ctccttcggt ttcatactta tgaataatga cgcccttgac tactcgattg
    16381 accgtgcctg ttggagatgg tgtatctggt ttattagcta ctttgattga agtatagagt
    16441 gcaatgattt gtgcagtcat aatcatcgct aatgcaagat aggcatcggg caatttactc
    16501 accgagtctt ccaatagcac agtcatacct gagaaatcaa tagtacccgg ttgcgcaatc
    16561 gcaatgacat tggcagtgac ccgatcttct tttatttcct ctaaaatatc taagtcataa
    16621 tttcgcgtat atggttgatt gctcacaaaa cctacaacga gtgtctcgct atttaaaaaa
    16681 gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa ttgtagcaac tttacctgct
    16741 gttaattcta aaatttttaa ttgtgcttct cttgtcgcac ctgccaaact tcctgaaccc
    16801 agataagcga tccgttcaaa aaaatcaggt aataactttt gaatttcttg atctcttgaa
    16861 agaagatctt gaccgatttt aactaattgt tcaagatatc tttctttttt ctccattgct
    16921 gttttatcaa agatccataa agcactcaat aacatacacg taaaacttcc tgtcatagca
    16981 aaacctgcat cgtttgctct ctctggcatc aataacagat aattctgttt atcatcctgt
    17041 gcgttttttg ccaacatccc ctctttagca caagtaatcg tcaaatgaaa caatgtttca
    17101 acttgttcat ttgcaatttg cacagccgct acactttctg gactgttgcc actgcgagca
    17161 aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt cttttggtga agctacaata
    17221 tctgtcgtgc caaaactgtg gaagataaaa aactgattgt ctccatgttc atttagatag
    17281 ggtaagagcg tatccccaat atattgagac gttcctgcac ccgtaaaaat aacacgtatt
    17341 cttcttccgt ttgcttcttt ttttatttga tcaaaaaata gagctaaatt ttcttggctt
    17401 tgcatcaatc tatcgattgt ttctttccag agatctggtt gttgttttat ttcttgtgtc
    17461 gtgattgctg cacctatttg ttttaatttg ttcttcgata gctcaaacat gttgttctcc
    17521 ctctcaatca ttttcggata tgccgaattt tataatgaaa ctggtccgct cttgctacac
    17581 tatctgtata ttcgataatc tcatttttca cgttataggt tatcctcgtc agatgtaaga
    17641 cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt tttttgcacg atacttgcat
    17701 aaaactcttc atcagctaat tgaacttgtt gttcataatc ctgtgaaaac acatcataca
    17761 acggtttact ttcaagtaac ggcaaatcaa gtcccataaa tagtttcgcc ggtagataag
    17821 aacgctcaac catcaatggc tcttcatctg ccaaacgcaa tcttttaagt ttaaatacag
    17881 tttcacctaa atggatgttc aaattcttag caattctctt attagcttcc atttgtctaa
    17941 attctaaaat gaccgtctta gggacgcgcc ctatttcgcg catttcttct gtaaagctat
    18001 atgctccagc taagttcgat gacttatcag acaaatcaga gacataggta ccttttccat
    18061 gtcttcggta aatataacct tgattttcca attcctgtaa agccagacgt acggtggtac
    18121 gactcacccc atagtgttga caaagttctc tttcagataa cagcttatca tgcggagtca
    18181 tctgtgtttc gatacgatcc ttcaataaat caactagttg ataatacaat gattttcctt
    18241 tatttgagct ctccataaaa atcacctctt ctttactggt tataaccaca acacaagaat
    18301 agtgaattat taatcatttg tcaaaggttt tttagaccaa aaaagctatt ataacagctc
    18361 tttaaagctt tatatcttga atagacaaag atacctcaaa aataccagtt accaataaaa
    18421 gaacagaaaa aaagacgaac cacataagcg atccgtcaaa atggtctaac ttatcgagca
    18481 gcgtgatcca cattaatccc tatgatattc ctacaactat cgttttatta cttccaatat
    18541 catttacaac aactaaaaat agcttattaa tgacaaatgt tattattagt tttagataac
    18601 ttttgcagtt ttatactttt tttctataaa atttgacaaa cattttttta aataactaat
    18661 tattttttga atctgtcact cagacaatcc tttctcttac ttgttaacat aaatagaagt
    18721 tgctgtttct tctaatagtg tacttaattc tttaatattt tgttttcctt cttctaacat
    18781 ccaactactc gctttttctt cgccttcggt aacaaaaggt gctactccct tcgcccatgt
    18841 tgctcgccca cataagacac cattgaatgt cgaacctgat tgttttgcaa aacgcaatgt
    18901 atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa ataaacggtt gttcagtcac
    18961 tttcgattgt tcatagaaat acgcggatgc ctctgcttga ctataacaag tatcttgccc
    19021 atatccttcc acaaatctca tattcactgg tacttctact ttcaatacat ccacttgata
    19081 acgttctttt gaaaattctc gcatggcttc aatcacttta tgcggtttta cttttgcata
    19141 gtcaagagaa gacggatcgc caatagttgc atcgtaagaa aggagctcta aaaagaaagg
    19201 tatgccttct gcttcgcatt ctgaaccaat ccgttctata actatttgtt tttgcacatt
    19261 aatctctact ggttcatcaa cgtcatagta caataaaaat ttacaagcat ccgcacccac
    19321 ttcttttaac cttttcactg accaactatt cagtaaatct ggtagtcggc ctggcatttt
    19381 cgcatcatag ccggtttttt cataagctag caacaaacca cattctttct ctcgaagcgc
    19441 tgctgcaggc agaccatatt ctggatcgag taaaatagcg gaagcatacg gagtcaggta
    19501 ctgagaaacc atctgtttaa attggatgat atcttctgat gtagcttctt tatattggtt
    19561 gatcattttc ttcaaagcac cacgttggtc gatagctagc gcgctaataa ttccagcttc
    19621 atccgtcaat cgcttcaaac gttcctgttt ttctatactc attttcttca tatatcttca
    19681 cctatttctt tagttaatat tgtagctatt tctatttttg attttgcagc tctcaactgt
    19741 tctgtaacct cttttcgcat caatgatcgt gcaacgagag acaataattt caaatgagtc
    19801 gttcctgctt ctgaatctgg gataaataac gcaaaaataa aatcaactga ctgtctatct
    19861 aaactgttcc attcaattcc ttttttcagt gtgacgataa tcaaactagg ttcattgatt
    19921 gtttgagact tcgcatgagg gatagcaaaa cctgacatta ttcctgtagt accttctttt
    19981 tctctttcca gaagtttttg atatacttct ttttcgtcat gagcgattcc taaacagaat
    20041 gtttgctttg ctaaaaactt aaatgcttct tcttgattat ctaatggctg ttgtaaaaaa
    20101 atgttctctt catgaatgat tgtcatgata aacctcctgc tcttccttct tcgataactg
    20161 tacgatttgt tgctttatta tccctcttct tgtctttatc agttactctt ttcttttgga
    20221 tcaaacctag tatcactcca ccaacgatcg aaccaataag aatcgctgta acccattgga
    20281 tttttcccgt cactacaggt aacactaaaa aaccgccatg tggggctggt acttggacac
    20341 caaacagata ggtcaagaca gcagcaattg atgagcctaa catcatcgtt ggtaatactc
    20401 ggatcgggtc tttcgcagca aacggaatag ctccttccgt aatatgagtt gatcctaaaa
    20461 taaaattaac caaacctgca ttacgatcat tagtatcaaa atatttacca aacaataaag
    20521 tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc tgacgcacca gccataaaag
    20581 tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata tgccgcctta ttaaccggtc
    20641 ctcccatatc aaacgcacac atacagccaa caatcacgcc aagaacaagt ggattcgaat
    20701 attcaaatcc agctaaaaag ctcatcattc cttcatttat agccttcatt ggacctgcca
    20761 gcaaagacat cacgatgcca ataaatgcta caccaataac tggatataag acgattgcct
    20821 tcaagccatc tagttgttta ggtaaaatta tcagtagctt ttgcaaaaat aagataaaat
    20881 aacctgctaa gaacccagct aaaatacctc ctaaaaatcc agtaccgcca ttcatggcta
    20941 cgattccacc aacaaatcca acgactaacc ctggacgttt cgcgatccct tctgcaatat
    21001 aagctgctaa aactggaacc atcattccca ttgctgcatc gtcaactgct tttaccattg
    21061 ccgctgtagc attgtattgt gcatgatctg gatcagaaga atagattccc caaacggcaa
    21121 atgaaatagc aatcaatact ccgccagcta ccacgaaagg caacatatga gagacaccgt
    21181 tcatcaaatt tttatagatg ctacgaccaa aattagcacc acttatcgct tctggttcta
    21241 aagtatcagc agcttcatta tctgctaaga cagtaccttt tcctgccaat gcttcgtcaa
    21301 tcagccgttg tgcatccttg attcctgtac tgacagaaac atcaatgact cgttttccca
    21361 caaatctttc tgaatggaca tctttgtctg cggcaataat gacagcatct gcctgtttga
    21421 tttcttcagc cgttaattca ttctcgatcc caatttgtcc atgggtctcc actttgatcg
    21481 tgatgccttt tttcctagct gcttgctcta atgcctcttg cgccatgtag gtgtgcgcaa
    21541 ttcctgtcgg acaaccggtt gctgcaatta gttgatattt tcccatcttt cgaacctctc
    21601 ttccttgatt ttgatttgtt tacttaattc ctccacatcc aaaaaatcag tcagtccttt
    21661 acgaaaagca gtagagctgc ctgctgctat gcttttacgt aacgtttcat caagcgatcg
    21721 tcgattcatg tatcctgcta aaaaagtgcc taacatggca tcacctgcac atgctgtatt
    21781 gacaactttt ccttttggcg agtttcctcg aatcaccctg tctttcgtaa aaagaatcgc
    21841 tcctttctca ccgagggata acaaaacatt ttctgcgcct gactctacta gtcgttgtcc
    21901 ataaacaaag taatcttcat ctgtaaccat ttttcgtccg aaccaagaag aaagttcttc
    21961 ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa tccatgactt cttgatcact
    22021 actatcaata atcaaacaaa tccgactgat ttctattaaa acctttggag acagccctct
    22081 tggtaaactc ccagaaacac aaagataatc ccctgcttgt aatgctctta tttgatttaa
    22141 aaaattatga acagctgtct gcggtatttc aggtccttga ttcactaatt tgtattcttc
    22201 tcccgtttga ttgacttgtg taaatacatt gatacgcgtc ataccaggca cttcaataaa
    22261 ctctgtttgg atttgtttct gctgtaaata gtcttctata tactttcctg tgaacccagc
    22321 agaaaaacct aatgccgtac ttgggaaatc tagcatcttt aatactagtg acacattcac
    22381 cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg tttaccataa aaggatgcat
    22441 ctcatctgtt tcaataaata gatcaattgc caaattcatc gtgcatgtgt agatagacat
    22501 aaccaacgac ctccttgatt ctattatgaa atacgcgaaa tagaaaatgt tttcttaatc
    22561 gtcccaacac aaagaaaacc tttacattta taaactcgtc gtgctttttt taactaactt
    22621 ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg aaacagagca gtacctctgg
    22681 aactttatcg aacacctcat cctagagatc cctaactatt ccatcgtgaa actaagtgaa
    22741 caagcaaatg tctcaacagc caccattgtg cggacaatga aaaagaaagg ctatgaaggg
    22801 tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca agaacatcaa tttttcattt
    22861 ttagataaag tagacaaagg aatccgccga gccattctaa aaaatgaaca agaagtggta
    22921 cgtacaatca atatgttgga aatcgggaac attgaagatg caatacaaag aatcaaattt
    22981 gctgaccggg tttttgtctt tgcccgagga ttttcagaaa tgattggcca agaaatgctg
    23041 gtaaaacttc aattaacggg aaaaaattgt cagatgcata ctgatccaga aattatcaaa
    23101 agcatcagtc ggaaactaac taagaaagac agcgttattt ttgtttcttt gaacggtgag
    23161 aaaacaaata aaaacatcaa aaataagatt tatgtcttta tttttcaatt ttttaaat
//
LOCUS       DAFCNS010000044        23009 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFCNS010000044 DAFCNS010000000
VERSION     DAFCNS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.044"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            476..727
                     /locus_tag="LNE23_001684"
     CDS             476..727
                     /locus_tag="LNE23_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931541.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            761..1039
                     /locus_tag="LNE23_001685"
     CDS             761..1039
                     /locus_tag="LNE23_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931542.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1636..1893
                     /locus_tag="LNE23_001686"
     CDS             1636..1893
                     /locus_tag="LNE23_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931543.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2006..2617
                     /locus_tag="LNE23_001687"
     CDS             2006..2617
                     /locus_tag="LNE23_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931544.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2796..3353
                     /locus_tag="LNE23_001688"
     CDS             2796..3353
                     /locus_tag="LNE23_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931545.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4052..4573)
                     /locus_tag="LNE23_001689"
     CDS             complement(4052..4573)
                     /locus_tag="LNE23_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK6931546.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4772..8464
                     /gene="nifJ"
                     /locus_tag="LNE23_001690"
     CDS             4772..8464
                     /gene="nifJ"
                     /locus_tag="LNE23_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBK6931547.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8653..9540
                     /locus_tag="LNE23_001691"
     CDS             8653..9540
                     /locus_tag="LNE23_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBK6931548.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9537..10706
                     /locus_tag="LNE23_001692"
     CDS             9537..10706
                     /locus_tag="LNE23_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931549.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10730..12085
                     /locus_tag="LNE23_001693"
     CDS             10730..12085
                     /locus_tag="LNE23_001693"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBK6931550.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12157..13962)
                     /gene="glmS"
                     /locus_tag="LNE23_001694"
     CDS             complement(12157..13962)
                     /gene="glmS"
                     /locus_tag="LNE23_001694"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBK6931551.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14371..14661
                     /locus_tag="LNE23_001695"
     CDS             14371..14661
                     /locus_tag="LNE23_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931552.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14674..15699
                     /gene="sppA"
                     /locus_tag="LNE23_001696"
     CDS             14674..15699
                     /gene="sppA"
                     /locus_tag="LNE23_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBK6931553.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15702..16361
                     /locus_tag="LNE23_001697"
     CDS             15702..16361
                     /locus_tag="LNE23_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK6931554.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16518..17450
                     /locus_tag="LNE23_001698"
     CDS             16518..17450
                     /locus_tag="LNE23_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931555.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17604..18893
                     /locus_tag="LNE23_001699"
     CDS             17604..18893
                     /locus_tag="LNE23_001699"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBK6931556.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19140..20390
                     /gene="clpX"
                     /locus_tag="LNE23_001700"
     CDS             19140..20390
                     /gene="clpX"
                     /locus_tag="LNE23_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBK6931557.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20473..21060
                     /locus_tag="LNE23_001701"
     CDS             20473..21060
                     /locus_tag="LNE23_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBK6931558.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(21134..21289)
                     /locus_tag="LNE23_001702"
     CDS             complement(21134..21289)
                     /locus_tag="LNE23_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931559.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            21425..22752
                     /locus_tag="LNE23_001703"
                     /pseudo
     CDS             21425..22752
                     /locus_tag="LNE23_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22763..22972
                     /locus_tag="LNE23_001704"
     CDS             22763..22972
                     /locus_tag="LNE23_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931560.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         7657 a         3917 c         4912 g         6523 t
ORIGIN      
        1 atacaaataa ttggtttatt ctggcaggcg taatgctttt ttctagtttc tatttattta
       61 gtaatgctct attagcacag cgagggaaat ataagtttaa aatattagat tagatattat
      121 ttttagaggg gataaatata aaatgaaaag aggttattta taatggaaaa agtattacca
      181 attaagagct actgtattcc aaggcttttc atcatagaat ttcaaaagta aagacatgtg
      241 tttcacaaat agtgtctatc ctggctaaat aattcttcca ctatctgctt taatttcagt
      301 agacttttcg gcaagttatt tgctaatgag atgaatagag attacaactc gcctataacc
      361 gcttaaaaca agctttttta aagtatagtt agcaagtcgt attcatatgt tcaaagagga
      421 gggcttatga actttgaatt cattccgtgt gaacagaaca gtagggggaa gtaaaatgaa
      481 agaaaagcct ataaataaat ggtcattgta taaaaattca atgtttggag ttgttatctt
      541 tggtgtttgt gctggattat tccttttcat attattacaa acggtgactc ttttctcttc
      601 taatcctact tatggatgga gagacagtat agccttaggg atgtttgcgg gaggatatgg
      661 attcttattg ttgttctcaa ttctcacact aagtccaatc catggactca ttcaactttg
      721 gaagtaagaa cgagccttaa acttttcgtt ctcggaagaa atggaaaaac acaacataac
      781 aggcagtatg tatgtggatg atcaatggtt tatcagcact gatgaggtaa aaatagctgc
      841 cattaggaga gattacctgc agaaaattca taaaataaaa gatcctaatg ggaaaaaggc
      901 aaggttaatt ataactttga taacatctaa tggtaagaag ttaaaacttg caggatcata
      961 tgataatgtt aaaaagttcg aacattggta tctgcaaaaa gacagcatta gtcttaataa
     1021 acaatttgaa aaaaattaat caaagtcatc ggcaacacga ataatttgac atcttgatat
     1081 tgtgatataa gcaaaaacgg catttctgag accgaacagc ataaaaaatt taaatgttgg
     1141 aatttatata agcaaaaccc aagaagctag atgcgataga gcatttaatt tcttggggtt
     1201 ttgcttggga atcagcatat caattaaaat gatcatctcg gtatttcaaa agtgggtacg
     1261 attacaaata gttggagcaa aatagtgggt aaaattacca ttccagtgtc tgtcttgcct
     1321 ctttttcgtc aatcacaaat agacgagatt cataggaaaa atcgatacag ggtatgattt
     1381 caatgatatt atgaccaatt acaaaataga ttgataaagc attttttgca tttttataat
     1441 agtttattga taataatgta tgagatacag tataatgatt taaaaggtaa cgtgttgtga
     1501 ccgaattaaa tgaattaatg cctatttttt atattcctta gtggacaaaa atggagacag
     1561 tgaagttagg agcattgtat cctttgttgc gtagttcaaa aaagtaagcg atttctataa
     1621 tggtcagggt aaacggtgag ggctgtccac gtaaatccta ctattgaaat cacaatgatc
     1681 ggacattcaa aaggtggtgc tgaagcagca aattcagtag ctataaatac gaatgcgatt
     1741 atatttaatc cagcgacagt aaatttatct gcttatgaat tagactcaaa cacatataca
     1801 gcaaacatgg tagcttatgt tgtcgaggga gatgcattaa atgttgtttt tggaggagta
     1861 tacacctatt ggagaattaa tcatgttaga tagacaatat gatgcacctt agtggagaat
     1921 gatatctcgc ataggtaact cagtaaaaaa tcaaggtatg gaagcaatta tctctgcatt
     1981 ggaagatcga tatagatagg cggatatgaa tagtaatagg gggaataaaa tggaatacaa
     2041 tggatcaatg agaattgtac aaattatatt ttggattaca tcgggaattg tgatcatagg
     2101 aggagttttt ttgatgttgc caagcttaat atttcctttc tttgcattaa tctcacctaa
     2161 aatacccgaa ccggaaatca cctatggaga atttccattt aggattgaat acgaactaga
     2221 tggacagatt aacataattg aagatactgt catagctgag tttaacgggt gtgagttcag
     2281 cgcaggttct atgaaaagag aacgaagatg gagaagtaga ttagctagtg gtagagaaga
     2341 tctacctttt ggggacgatc taggaatcta ttttagtcga ggaagtgcgc aatattatat
     2401 gggggaaaat gtacaatcaa tgtcattaaa accacatata gcccttagaa atctcgagga
     2461 aggttttaaa agagaggtag actttatatt aggagaaagt ggatacataa gaactgtact
     2521 taatttcgga gaagcgcaag aagttttgac acaatacgga attaccttaa tcaactggga
     2581 aatatctgaa ccgattgtta acaattttgg tgattagaaa acaattacga taaatagaaa
     2641 caagccaaat catcagcaag taaaagctga tgatttggcc tttcttatac gagtagtagg
     2701 aattcatagc gaatattatt acgaccagta gatattttga tattaaagga gtattacgaa
     2761 ctaatatgtg agaatgtaga ggtgatgtgc gaataatgag aaatttatca aatagaaaca
     2821 aaatattaat cattattgtt gttatagctg tgtttcatct gggaacgaat gcggttttaa
     2881 gtagaataat attgggtcct aagccaccaa gaccagaaat tacccgaggg gagtttgatt
     2941 ttagattaga atatgaagta gatggggaaa ggattgtgat agaagataca attgttgcat
     3001 tatttgatgg attttctgca gatgcaggta gcatggcatg gtatcgcacg tggcgtctac
     3061 atttagcaag tgatagacga tcacgaaata tcttattaga tgaattagag gatggtcgaa
     3121 gaatatatta tgtcccagaa agtgctaact attctatggg agatgtacag aaaaaaagag
     3181 agcctaatcc taattggtat ccatttaatg gcgtaacaat agaatatcca cgcaataaaa
     3241 cacctgaaat aggtgcaaaa tttataagtg gtttagaaga tctgtacgat aggtttggaa
     3301 tccgtcttat tagttgggaa cacgatccac caatagaaaa tcgctttgaa taatcgaaat
     3361 ctactaatcc atttcatcag ccagtgaatg ttgtaatgga acaatttggt ttagtgttca
     3421 aaaccattga aatactaggc tgtgatcagc aaaaatgaat ctgaataaaa aagagagcaa
     3481 ctattagctg agaggagtta atagttgtta gctttatacg aatcaaagtg gcaaatacag
     3541 atgcgtatgc atttgtgttt gccatcttgc gtttggtaga cgagataggt ataagctaaa
     3601 gaaacaactt gtttaaaata aacgacctag ctcaacaaat gaccatctaa tcaactaaca
     3661 gcatacataa aagcaactat ttttgaagta gaattgaaat cgggataact taaaaagtga
     3721 ttgggatact agtgagaatc accgtacata aatattactc gttaagggaa attgagaaat
     3781 tttgtatgaa aatttaagaa ttagttagta tgcgcaagtc aaaaatatga ataaattatt
     3841 tggtggagat aatttgctta gcttgtcata agaaatgaga taatctggca gatgcaagct
     3901 tttggacacc atttattcgg aataaggagg ctgggacaga agcgtttaac tccaagaaat
     3961 aagaaggagt tgcttctgct tccgccgttt atacgtgttt agagcgtgag acaaaagtgt
     4021 tctttacttt tgtcccacgc tccttatttt tttattttgt ttggatcaat tcttttaatt
     4081 cctctattga aatagattca tcaaagattt ctttacccaa gatgacagtt ggaacaaatc
     4141 gaatatgtgc ttgctcagct tcgttgatga cagcttggct cagctgttcg tctttttgtt
     4201 ctgtcagctt tagtttatct actgcaaaat ccgctacttc ttggagagaa agatgtttcc
     4261 actcgtcttg agtatcgaat atttgtttga tctctttgat cgctttttgg ccatcgctga
     4321 ttgtgagata ccggtgcatc acgttccctc gctgtaagct ttctttttct ttatcgaaca
     4381 atttgatcac tcgctgcaat tgcccgcttt ggactgcttc ttctaatagc tcataagatt
     4441 cttcaaacca ttgtcggcaa tacggacaag ctaagttgat gaattcgacc atttttttag
     4501 gagcattcga tgaaccataa aggattcctt tttgtgtatt tgttttcgtt gcatcaatca
     4561 ctgatatatc catgttaaaa cacctctttc gcttatagtt tatacatgtc tgacaaaaaa
     4621 aggcaacgat ttagactgtt tgtctaaaaa ttagatgacc aattcaaata gatacatgta
     4681 aaatattttt ataaataaaa gaaaaaaatg gcatgtttta cgttataatg gaacagataa
     4741 taagagagaa tatgaacaag gatgatgaat catgcgaaaa atgaaaacaa tggatggcaa
     4801 tacggcagcc gcatatattt cttatgcatt taccgagatt gctgcaatat atccaataac
     4861 acccagttcg accatggctg aattagtaga tcaatggtca gcagcaggaa aagaaaacgt
     4921 attcggtcaa ccagtaaaag tagtagaaat gcaatccgaa gcaggagcag ctggtgtggt
     4981 ccatggatca ttgaaaactg gtgcactgac aactacatat accgcttcac aagggctgct
     5041 tttgatgata ccgaatatgt acaagattgc tggggaacta ctgccaagtg ttttccacgt
     5101 tgctagtcgg gcagtgacga caaatgcgtt gaatattttc ggagatcacg gagacgtgat
     5161 ggctgcaaga caaacaggat ttgctatgtt agctgaaagc agtgtacaag aagtgatgga
     5221 tttagcacct gttgcccatc ttgcttctat tgaaggagaa gtcccttttt tgaatttctt
     5281 tgatggattc cgtacgagtc acgagattca aaaaatcgaa gttttggaat atgaagaatt
     5341 agctcctctg atcaatcagg agaaattagc ttcatttcgt aaacgaggga tgaatcccaa
     5401 tcatccgact gttagtggta cgaaccagaa tccagacatc cattttcaac aacgggaaac
     5461 gatcaacccc tattatgata aattacctga aatcgttcaa aaatatatga aagagatcaa
     5521 ccgattacga ggaacttctt atgacttggt gacttattac ggagcagacg atgcggaaga
     5581 agtcattgtt tctatgggat cagttgcaca gacgattgaa caaacgattg attacttgaa
     5641 tgcaaacgga cggaaaacag gttttttgaa tatccatctt tatcgtcctt ttccagtgga
     5701 aacattcgtg caaaagttgc ctcatacagt aaaaacaatt gctgttcttg atcgaagtaa
     5761 agaaccaggg gcaggtggag aacctctctt attagacgtt caaagtgcgt tatatgattc
     5821 tgaacttcgc ccggctgtta ttggtggtcg ctacggattg gggtctaaag atgtgactcc
     5881 cgaccaaatc tcagcagttt ttgatgaatt gaaaaaagac ccaagcatac gcaaaaaacg
     5941 gtttaccata gggatcgtgg atgatgtgac ttatcaatct ttggaaccaa aggaaagtct
     6001 ggatttgaca gaaccgcaaa cattccaggc caagttttgg ggatttggat ctgacggaac
     6061 agttggagca aataaatcag ctatcaagat tattggagat cacaccgata aatatgcgca
     6121 aggctatttt tattatgatt cgaaaaaatc tggcggtctg actgtctctc atcttcgttt
     6181 tggagatacg ccgatacgtt cagcgtatct tgtcgagcat gcggaccttg ttgcctgtca
     6241 cacaccagct tacctgcata gctatgactt ggtcaaagga ttgaagccag gtggcatttt
     6301 tttattgaat actctttgga gcgacgaaca attagaaacg catctgccct taaaattaaa
     6361 acgttacttg gctgaaaata acattagatt ctatacgatc aatgccatga gattggccca
     6421 agaagtcggt ttgggacgcc ggatcaatac ggcaatggaa acggcttttt ttaaattagc
     6481 tgacatcatt ccttttgatg aggtgcttcc gctgctaaaa gaagaagccc tgaaaagcta
     6541 tggacataaa tcaatggcta tcgtagaaaa aaacatacaa gcaatcgatc ggacgaccga
     6601 actgcttcat caagtcgcga tcccagacca ttggagaacg ttggagatgc ctgaaaaaat
     6661 caataaaagg aacactggtt atatacaaga aatacttgaa ccagtgaatg cacaggaagg
     6721 aaatcaactc tctgttggta cacttgtagc aaatggaatg acaactggcg aaatgccttt
     6781 aggaacggct tctatggaaa agcggggcgt cgctttagaa gtacctgagt ggattagtga
     6841 tcgctgtacg atgtgtaatg aatgtgcctt tgtttgtcct catgctgcca tccgtccatt
     6901 tttagcagat gaagaggaaa tggaagaagc gccagaagga tttatcgtga gagaaatgcg
     6961 aggcgcagac ggcgtaaaat accgtatcca agtttcagtg gaagactgta caggttgtgg
     7021 tctgtgtgta gaggcttgtc ccgcaaaaga aaaagcttta gtgatgaagc cttatgaaga
     7081 acagaaagaa caagctgtga attgggcttt tgcaatgaca ttaagacaaa aagcaaatcc
     7141 agtcaaagga aagaatactg tcttgtctac acaattcaat caaccgcttc ttgaattctc
     7201 gggcgcttgc tcaggctgtg gcgaaacacc ttatgtaaaa ttattgacac aaatgttcgg
     7261 tgatcgaatg atgatcgcga atgcgacagg ctgttcttct atttggggcg gtgcttctcc
     7321 tgcttctcct tatacgacca atgaatgtgg tcaagggcct gcatggtcaa attctttatt
     7381 ggaagataat gctgaatttg gttatggtat gcttctagcg gcacagactc gtcgcgaggc
     7441 tttggcaagt tcaatcaaag aagtattgcc aaaagtatca gaagaactac gagaattgat
     7501 ggaagactgg ttagtacaca tgtttgagag tgaaggaacg cagcaaagag cagcaaaact
     7561 gcaagctgcg atcgaaagcg aacgatcaat tttccctgaa ctggaggata tatacaagag
     7621 aaaggatcta ttcgtaaaaa acagccaatg gatgattggt ggagacggat gggcttacga
     7681 cattggtttt ggcgggatcg atcatgtact tgccagcgga gcagacgtga atatccttgt
     7741 tttggataac gaagtctatt ccaatactgg cggtcaaacc tcaaaagcaa caccagcttc
     7801 ggcaatcgct aaattttcgg ctagtggaaa gtatgcttca aagaaagatc taggaatgat
     7861 ggcgatgaca tatggaaatg tttatgtggc gcaaattgct tcaggtgcca atcaaatgca
     7921 aacgatcaag gcatttgaag aagcagaaaa gtatcctggt ccttctctga ttattgccta
     7981 tacgccttgt atcacacatg gcttgcttgg cggaatgaaa gaatcgctta aagaagcaaa
     8041 agaagctgtt tcttctggtt attggtcatt atatcgatat aaccctacaa tggtcgaaaa
     8101 aaacaagaac ccaatgactt tggactacaa aaaacctgat ttctctgcta tgcctgattt
     8161 catgaggaaa caagttcgct tttcctcgct agataacgca aatccaaatc ttgcaaaagc
     8221 tttatttgaa aagactgtcc acgacgctaa aacacgtttt tacaactatg ctgcactgac
     8281 gggagaatta gaaaaaatca agaaacgact tgaaccagag gaatctatcg atgtacccgt
     8341 taagactaga aaagaaaaaa cgacggacct tgaagcagaa gaaagaagag ctgctagaag
     8401 agctgccaga gcagaacgac gtaaaaaaat ggaacaagct acaaaagaaa aaaaggaaaa
     8461 ataacagaaa aaatataatg aataattctc tgaagtttaa aaatatttat tgcagagaaa
     8521 aataaaacgc atacttgcaa ttttcttgaa aattatgcgt ttttctcaag aaaaatacga
     8581 gcatatcagc ctctaaaatt aaaacatatg atatactaga gaggaaatct caaaagagaa
     8641 gagaggcttt ttatgtcatt tcaaatcggg gaacttttta atttggacta ttggcagcaa
     8701 atgttttccg gtgacttgtt ctctctgaat tttttggtga acatacttga tattctggtt
     8761 gtctggtatc ttgtatacaa actgatccaa ctgctgcgag gcacaaaagc gattcagctt
     8821 ctgaaaggtg tagctatttt tattgttatc cgaatcgtag cggaaatcat tggtctgcac
     8881 accttgtctt ggctgatgaa ccaagtcatt acgtacgggg tgatagctgc tatcgtgatt
     8941 tttcagccgg aaatccggag aggactggaa catcttggac gcagttcgct tttcaaacaa
     9001 tcaaaaagcg aacagcagga agatgagaag atgatcttgt cttttgacaa agccatccaa
     9061 tatatgtcca agcgaaaaat cggtgcgttg atcactatcg aacgtcatac tggattagat
     9121 gagtatattg agacagggat tgctttagat gcagatatca ctggggagct gctgatcaat
     9181 atctttattc ctaatacacc gctgcatgac ggtgcagtta ttgtaaaaga aggaaagatc
     9241 gcagtagcaa gcgcctatct tcctctatca gaaagtatgt tgatccctaa agaattcggg
     9301 actcgacacc gggcagctgt cggtataagc gaagtcagtg acgcgattac catcgttgtc
     9361 tctgaagaaa ccggggacgt cagcatcacg ttagataatg aactgatggc tggcttatcc
     9421 cagcaagaat atttggcaat cttgcgtaga gaactaattg ctgaagagaa aacaaaaaac
     9481 aagaaaaacg tgattcaatc tttcctagac ggtatgacta agggagggga tcgcaaatga
     9541 tctcaaaaga gaaacggaca agcattcttt acagttttat tgcattgctg ttcagtattg
     9601 cgctgtactt caatgcaaat ggccagtccg ttccaaatac gctttctgga aatgaagcgt
     9661 ataatcaaac agttacgggg gtgccgatca agatttctta tgattcaaag cgctactata
     9721 tccatggata tgaaaatgca gtgactgtca aactttccag tgcaaaccga gttcagttga
     9781 atgcagaggc aaacgaagat acacggatgt tccgcgtcac agcagatatc actaagctgg
     9841 gcgaaggaac acatgaagtt ccgctaaaaa ttcaaaattt gagcagtgct gtcacagcaa
     9901 agatcgaacc atcgactatt acagtaacgg ttgagaaaaa ggtaataaaa gatttcgatg
     9961 tggaaacgtt gattccttca acgttcacac catctggata tgaacttgat gatacttccg
    10021 ttagtcctaa aaaagtctct atcactacag gtgacaaaac attggcagaa atcaaacgag
    10081 tagtagctga tgtggacgct tcgatggtca cagatgatgg gattaatagt gaggtcccaa
    10141 tacaggcgct gaatgcagca ggggaagtat taagtatcgt ctctgatcca gtgcaagtaa
    10201 cagtgagagc tgattcgatc aagccaagca agtcggtacg actttatggg atacagcaag
    10261 gaacaccagc tgccggggta gaaagctatg atttcagttt ttcaaaactg gaagcagaag
    10321 taagcggctc tagcgatttg cttgcatcga tcggtgacag tattgctgta ccgatcaatg
    10381 tctcaggtat caatcatcga acaactcgta aaattgaaat accagtagaa gaggggttaa
    10441 gtatcaagcc cgaatcagtt agtgtggaga ttacacccgt gttgcaaacg tcaagcagta
    10501 gctctgcttc gacgagcagt cggacacaag aaaatacaag tacgagtaga agtatttcat
    10561 ctgctgattc aggaacggaa ccatcgacaa caaccatttc ttccagttcg gaatctggga
    10621 caagcagctc gaccacaagt acagaagaaa catcagaaac aagcgaatca acacaaatga
    10681 gcagaacaaa ttcctcagaa aattaagtta aaggagtaat tttacaacta tgggtaaata
    10741 ttttggaaca gacggcgtac gcggtgaagc caacaaggaa cttacgccag aacttgcatt
    10801 taagctggga cggtgcggag gttatgtcct aagccagcat gaagaagaag gaaaacgccc
    10861 acgcgtgtta gtagggcggg atacaagaat ctcaggtcaa atgttagaag aagcgctgat
    10921 ttcaggactt ttatcggtgg gtatcgaagt gttccagcta ggtgtgatct ctacaccagg
    10981 tgttgcttat ctgacacgtt tgcaaaaagc aagcgcaggg gtcatgattt ctgcttctca
    11041 taatccagca caagataatg gaatcaaatt tttcggtgca gatggtttta aactagtaga
    11101 tgaccaagag ctagaaattg aagcattatt agatgctgaa gtggatgaat tgcctcgtcc
    11161 atcagctgaa ggattgggaa ctgtagaaga atttccagaa ggcttattga aatattctca
    11221 attccttgta caaacgatca acggtgacct ttccggtttg accatttgtg tcgatgcagc
    11281 aaacggagca acttcaacaa gtgttaatcg tttgtttgcc gatcttgaaa cagattttta
    11341 tacaatgggc acaaatccag acgggttaaa tatcaatgac ggcgtaggct ctacccatcc
    11401 agaacgttta gctgagatgg tcgttgaaaa aggagcagat gctggactag ctttcgatgg
    11461 cgatggcgat cgtattattg cggtggatga acttggaaac attgtcgacg gtgataaaat
    11521 catgtttatc tgtgctaaat acttggcaga aaaaaatcga ttgaaaaaag atacgatcgt
    11581 tacgactgtc atgagtaact taggattcca caaagcggtg gaaggaatcg gtttgaaaga
    11641 tgtcgttaca caagttggtg atcgttacgt tgtagaagaa atgcgcaaaa acgattataa
    11701 ctttggcggc gagcagtctg ggcacatgat tttcttagat tttaacacaa ctggagatgg
    11761 gatgctttca ggaattcagt tactgaatat catgaaacag acaggtaaga aattatcaga
    11821 gcttgcagca gaggtaacaa tctatccgca aaaattagtg aatatccgcg taagtgataa
    11881 aaacggtgcg atggatgttc cggcaatcaa aaaagtgatt gaagaggcag aagctgaaat
    11941 gggatcagaa ggaagaatcc tagttcgtcc ttccggtaca gaaccattac ttcgagtgat
    12001 ggcagaagcg cctactgatg agaaagttaa ttactatgtc gataaaatcg cggcagtagt
    12061 caaagaagaa atcggactgg attaagagat attttcataa aaaataaaag aacaaggacc
    12121 agtgtcagat gtctgacact ggtccttgtt cttttattat tctactgtca ctgatttcgc
    12181 taaattacgt ggtttgtcaa cgtcgaatcc ccgtaatagc gttgcgtaat atgcaagtaa
    12241 ttgagtcggt acgactgtgg ttagagtagt tagataaggg tggacagttg gtagcaccag
    12301 ctggtcactt tccttttcta atccctctga gacaataact agtgttttag cgccacggct
    12361 ttcgacttct ttcaggtttc ctcgagtatg cgcaccagtc acttcttcac tgatgattcc
    12421 gatcactggt gttccttctt cgatcaaagc aatcgttcca tgtttcaatt cgccagcggc
    12481 aaaaccttct gcctggatat aactgatttc tttcaatttc aaagcagctt caagagaaac
    12541 gttgtagtca gcaccacgtc caatgtaaaa ggcatttctt gtatcgctga gatatacttc
    12601 tgccaactct tctaaataat ctttttgggc gatgaccgat tccatcgcat tagcaactaa
    12661 actcaattca tgagtgatat cgaatacaag agaagtttca aattcttttt tatcaccgat
    12721 agctttggct aataaagtta gtacagctat ttgtgcagta taagctttcg ttgatgctac
    12781 tgcgatttcg ggtccagcat gcaatagcag tgtaaaggat gcttcccgcg aaagagtaga
    12841 accagcaaca tttgtaatcg taagtgaagg gtagccgaga cggttgattt ttaccagcac
    12901 ttggcggctg tctgctgttt cgccactttg actcaagaaa ataaagaatg gtttttttga
    12961 aagaagaggt gtgttatagc caaattcact ggctaaatga acctcaacag gaatctgtgt
    13021 caatttttcc aaagtctgtt tacctgctaa ccccgcatgg taactagttc cgcaagcaac
    13081 gatatagatt cgatcgctat cagacaatgt atcgaccagc ttttgatcaa gagagacacg
    13141 attgtcttct gttacgtatt tttgagcgat acgacgcatg ataataggct gttcgtcgat
    13201 ttctttcagc atataaaaag gataggtacc tttctcgata tctgaagcat ctacttgtgc
    13261 ttcgaatgaa gaacgttcta cgatgtcacc tgcaaaattt tgaataataa ctttttctga
    13321 tgtgacggta accatctctt catcttcaat ctcaacgaat tccttcgtat gagcaagcat
    13381 agccattgca tcgctggcaa tcacattgaa tccatcgccg agtccgatta atagtggact
    13441 tttattttta gcgacgtaaa taacatcagg tgctgtccga tcaatcaaag cgaaagcgta
    13501 agaacctttg atgacccgca aagcttttaa aaacgcctct tgcgttgtag tatccttcgc
    13561 gaaagtttca attagatgag cgataatctc agtatcggtc tcaccaataa agtggtcatt
    13621 tgctaaataa gcaattttaa gttcttcaaa gttttcgatt acgccgttgt gaacgagcac
    13681 gaagcgtcca ctttgggaag tatgtggatg ggcgttttct tcagaaggtt gcccgtgagt
    13741 tgcccatcgt gtatgaccaa tacctatatt tcctgtagta tcaatattta ttttgttttt
    13801 caaattttga attctgcctt gtgctttgac cagataatca gttgtgccat ctgagacaaa
    13861 gataccagct gaatcataac cacggtattc taatttttct agaccgtcaa tcaatcctgg
    13921 tgttacattt tttaatccaa tcattccaac aattccgcac ataaaaaaca tcctactttc
    13981 tataatgaat tcgtatgaat gcttccagct gcaggaaaat ccttttgttt tatctttgca
    14041 gacatttttt gtggattttc tgtagagtgc agtccctagg gccatccgcc gaatctttcg
    14101 ataaaccctc tcctcgtcat ctgtttacac agacctggcg ctatcctaaa aggtataact
    14161 atcctccttt ttgtttttta tctggaaaca tttaaaatca taaccttttg cgaatgttct
    14221 tgtcaacaaa aaaagaataa aaaataaatt ggtatatatc aaattctttg tcaaatgtcc
    14281 tttttatgtc aaaaaataat gggtttttat ctatcatctt tttcaggagt tcggtacaat
    14341 agaaagtaat cggatagagg aggagtaaaa atggattcaa aaattgagat catgacactg
    14401 ggaatgttaa aaaaacaatt gtccaaattc gaagcatcag ctggtgtatc agatgatacg
    14461 aaaatatttt tagatactgg ctgggatagc atccaagaaa tcgcacctga tgccttggaa
    14521 gttgctcaag ctcgtgagtt tacggtagaa gatgaattaa caaaagagtc gttttccggg
    14581 tatgcgcggg aagaaaaagc agagcggttc gacacttctg agcagtcaga aactgtgatc
    14641 gtcataaaaa acttgtatta gaaaggagaa agaatgaata aaagacgttg ggcagcagtt
    14701 ctgattgctg caggattatt tgtggtttcg ttagtttcag ctagtttgac taagcctcag
    14761 gaaaacgatc aaattgaggg attgaacagt tggttatatg gggatgaaga actagctcca
    14821 aatgtgttgg aagagggaga cagtacgcaa aaaatcgtaa aattatcagt agatggaacg
    14881 attgctgcgg gagggtcttc aggtttattt gcttctgaag gttacaatca taaaaatttt
    14941 atggaacaac tgaaggcgat tgaagaagat ccaacagtca aaggtatctt tttagaagta
    15001 aattctcctg gtggcggtgt ctacgaaagt gcggaaatcg ctaagaaatt ggatacgatt
    15061 cgtaaagaac atgatatccc aatgtatgtc agcatgaaaa acatggcagc tagcggtggc
    15121 tactatattt ctgctcaagc agataaaatc tttgcaacag aagaaacagt gaccggctca
    15181 attggtgtga tcatgtcagg attgaattat tcaggacttc tagaaaaact gggtgtggaa
    15241 gatacaacag ttaaaagcgg agcgttgaaa gatatgggtt caggaacgcg accagagaca
    15301 aaagaagaag aagcggtgct tcaagcatat atcgacagcg catatagtcg ttttgtagca
    15361 atcgtcagcg agggacggaa taaatctgaa gaagaagtca aaaaaattgc tgatggaagg
    15421 atttatgatg gtgtccaagc aaaagaagta ggtttagtcg atgaattggg atttcctgaa
    15481 gatgctttaa aagctatacg aaaggaacaa cagcttgaag atgcggaact agtagaatat
    15541 tcttccacgc catctgggtt tgctagtaca tggttaggaa gtaaactagc agaattccag
    15601 ggattgaagc cttctgagac tagtcagatc ttatccgttc ttgagaatct agggacagcg
    15661 gaatcaccaa aagcaatgta ttattacgga ggtgagtaag catgtcagag ctttcaaatg
    15721 aaaataagga acaagaaaaa gataagcaac agcagcctgc tgtaggacct gactccaact
    15781 tttcccaaaa ggtactgagc tcttttgaaa gagagccttt gacgcccgaa gaaatcaaaa
    15841 agaaacaacg taaatggaaa aagtatacgg acacaagaaa tgaaccgcca aaagcaaatg
    15901 attttcctaa gtatttctat gcaggttttt ttatccgaat gtttgccttc ttggtcgatc
    15961 ttttgtgtat cggagctatt acgcgtttaa cacttggtat tgccaaaaat ctaggttggc
    16021 ttaatttttc agattcttat ctgagtctct acggattcac agccttactt atttatctag
    16081 cgtattttat tttattgacg aaattcaatc atggccaaac gattgggaaa atgatctttg
    16141 gtattcgagt catcagcttt gatgaagaag aacttagctg gtcaaccgtt ttagttagag
    16201 aaggtgcttg ccggttcatt ctaaaatttc ctttgctgat gttagggtat cttccagcag
    16261 tctttaatca gaaaaagcaa catctgggcg attttttcac taatacaagt gttgttacga
    16321 tcaatctgat caaagcgttc aaccaggaag tcaacagctg atgagcaata aaaagcagta
    16381 acagttgaaa gcaccttcta aaagtcagat ctaaaatcta gcttttagag ggtgcttcag
    16441 ttatttttaa ttgtcatgct ttttttgttt tgctactatt gaacatgagc tgaatatgag
    16501 caagaggagg acactgcgtg aagccgattg attttccaga aaaatatgag aatttaatgc
    16561 gagtggcaca acaagcacta gcaaaccagc aatatcagca ggcaaaagaa ttatttcaac
    16621 gagcttacga gttgaaagaa agctttgagg ctaatagcct gcttgttttt tgtctttacg
    16681 agttggacga gaaaaaagaa gcattgaagc aagcactctt gcatgaaaaa cagtatcttc
    16741 aaaatgagga gtttgctgaa ttttattttg acttgttaat cagtacacaa gattttttat
    16801 atgctagaaa attgatagca agtacagatt tttatgaagc tttcgagcag cgggtcattg
    16861 aaaaaatcca atttgcagaa gaattatccg gacaaatgga gaggcagaag atcaaggcac
    16921 tccacaataa atcggaggaa cttccttcgt tggaacctgc acgtcagctt gcgtctattg
    16981 atgagatcga acagcttcct tatcatgagt ttatccaaac agcgagtaaa cttattgtat
    17041 cgccagaggt ccatatttta gcaagggcaa aattactgga gacacttagg caattgaacg
    17101 aatgccatcc tgttttctat ttgacgattg aagaaaagtt agtcaaagtc ataccaaaag
    17161 atctgccaaa gccgcagcag caggcaagtt accagcaatt atgtgtattt tcagatcgtt
    17221 acgggaatga agatgcgctg cttagttccg tgttaaaaga agaattcacg ttgcaatctg
    17281 caattgttta tcctgtctat gatacataca tcgaagaccc gaagcgctgg tttcaactga
    17341 ctgtagaggc gtgtacagga aaaacgatgt atgaaggtac agaagatgaa aaacaagatt
    17401 ttttaaaaaa aagagaaaaa attttgcagc aaatgatctt cttccattag attttatcag
    17461 tgaaaaagat aaaaatgtta tgaaaaacaa ggattctatc cattcaatat gtttacaatt
    17521 tgaaagggtt agtgtactat ttaaaggtat gcatgaaacg tttttgatgt ataaaatgaa
    17581 tttctaaact cggagggaat taaatgactg caacatttga aaaaaaaggc acgaatgatg
    17641 gtgttttaaa atttacgatt gcgcaagacg aaatccaaaa aggattaaca actgcgttta
    17701 ataaagtgaa aaaaagcttg aacgtacctg gattccgtaa agggaaagtt tctcgtcaag
    17761 tattcaaccg tatgtacggt gaagaagctt tatatgaaga tgcattgaat gctgtattac
    17821 cagaagctta tgaagctgct gtcaaagaag ctggcatcga tcctgtttct caaccaaaaa
    17881 tcgatgttga aagcatggaa aaaggtcaag actgggtaat cactgctgaa gtaacagtga
    17941 aaccagaagt aaaattagga caatataaag atctagttgt tgaaaaacaa gaccgtgaag
    18001 taacagatgc tgacgtggat gcacgtatca aacgtgaaca agaagcacaa gctgaattag
    18061 ttatcaaaga agatgctgca gctgaaaatg gcgatacagt agtaatcgac ttcgaaggct
    18121 ttgtagatgg cgaagcgttt gatggcggaa aaggcgaaaa ttactcactt gaattaggtt
    18181 caggctcatt cattccaggt tttgaagatc aattagttgg tcataaatca ggtgaaagtc
    18241 tagatgtaaa tgtaactttc ccagaagatt atcaagcaga agatcttgct ggaaaagaag
    18301 cagtcttcaa agtgacaatc catgaagtaa aagcaaaaga agtgcctgaa ttagatgacg
    18361 aattcgcaaa agatgttgat gacgaagtat caacattaga cgaattgaaa gaaaaatatc
    18421 gcaaagaatt agtagaaaca aaagaaaaag cagcggacga tgcaaaagat gaagcagcga
    18481 tccgtatggc tgttgaaaac gcagaaatcg ttgaattgcc acacgtgatg gttcatgatg
    18541 aagtacatcg ttcaatggac gaattcttga acaacatgca acgtcaaggt atctctcctg
    18601 aaatgtacta tcaattaact ggttctacag aagaagactt gcataaacaa ttcgaaggtg
    18661 aagcagaaat gcgcacaaga acaaatcttg tgatcgaagc aattgcagct gctgaaaatc
    18721 ttcaagcaac agaagaagaa atcaatgcag aaatccaaga attatcagaa acatacaaca
    18781 tgccaatcga acaaatcaaa cgcgtgttga cagaagatat gctggaacat gatgtgacaa
    18841 tgaaaaaagc aatcgaattg atcacaggta cagcgacaga agcgaaagaa tagaaatcaa
    18901 gtaataaatc atttctgaag aagctacttc tagaaaattg tttattcgaa ataagggact
    18961 gtgacacgat tttgtcacag ttccttattt tttgttaaga gtaaaaaatg caaaggtata
    19021 ggtcagttga tcctttgtta aacacttttt ctttttacta aactatgtta tgatagtaaa
    19081 agactgtaaa gtctttgata gtttcttgca tactttttgc aagttaaggg gtgaagacca
    19141 tgtatgacaa tccaagtagc aatgagaccg tacgctgttc tttttgcggg aaaacccaag
    19201 aagaagtgaa aaagatcgta gctggtccag gggtctacat ctgtaatgaa tgtatcgacc
    19261 tatgtaaaga aatcattgat gaagaatttt atgaagaagc tgtacgcgaa ttcactgaag
    19321 tccctaaacc actagaaatt cttgaagtat tgaataacta cgtgattgga caagatcgcg
    19381 caaaacgctc gttagctgtt gccgtataca atcactataa acgtgtcaat cagcaagcac
    19441 aagaaaacac agatgatgtt gaactgcaaa aaagcaatat ctgcttgatc ggacctacag
    19501 ggtctggtaa aacattttta gcacaaacat tagcaaaaac attgaatgta ccttttgcaa
    19561 tcgcagatgc gacaagtttg acagaagctg gttatgtggg ggaagatgta gaaaacattt
    19621 tgcttaaatt gcttcaatct gctgattaca atgtggaact tgcagaaaaa gggatcatct
    19681 atattgatga gatcgataaa attgcgcgta aaagcgaaaa tgtatctatt actcgtgacg
    19741 tttccggcga aggtgtccaa caagccttgc tgaaaattct agaaggaact gttgcaagtg
    19801 tgccacctca gggcggacgt aaacatccac atcaagaatt tattcagatt gatacgacga
    19861 acatattgtt catcgtaggt ggtgcatttg atggcatcga aacgattgtg aagaatcgta
    19921 tgggcgaaaa gacgattgga tttggtacac aaaaccaaaa actttctgaa gacgaaagtg
    19981 tcatgcagca tatcattcct gaagatttac taaaattcgg attgatccct gagtttatcg
    20041 gacgattacc tgttatggca gctttagaga aattgacaac agatgatctg gtacggatct
    20101 tgactgagcc gaaaaatgcg ctagtcaaac aatatcaaaa acttctttct ctagataata
    20161 ccgaacttga attcgaacct gaagcgttga gagccattgc gaaaaaagca atcgaacgca
    20221 ataccggtgc tcgaggactt cgttccatca ttgaagaaat catgatggat gtgatgtttg
    20281 atatcccttc aaatgaaaac atcgaaaaag tgattatcac aaaagaagca gctgaattgt
    20341 ctggtgagcc aacagtaatc tataaagatt cagagaaaaa agcgggttga tgaaaagagg
    20401 ctgccgtaaa agtcattttg gcttttgcga cagccgcttg ctttattcat caccttgtta
    20461 aggagagaga ccatgaaagt ccatcaagct gaaattatca taagtgcagt ggcaccaaaa
    20521 caatatccag atacacaatt gccagaaata gctctggcag gacgatcaaa tgtagggaaa
    20581 tcttctttta tcaacacatt gattgatcga aagaatttag cccgtacttc cggtaagcca
    20641 ggaaagaccc agacattgaa tttttatttg atcgagaacg cattacattt tgtcgatgtt
    20701 ccgggatatg ggtatgcaaa agtatccaaa actgagcgag caaaatgggg aaagatgatc
    20761 gagacatatt taacgactag agaccagcta cgtgcggtcg tttctttagt agatttgcgt
    20821 cataagcctt ctcaagaaga tatccagatg tatcaatttt tgaagtatta tgatatccct
    20881 gtaattgttg ttgccactaa ggcagataag atccctcgtg gtaaatggaa caagcatgag
    20941 agcgaaatca aaaaagcgtt ggattttgat cgcaatgaca cgtttattct cttttcttcc
    21001 gttacaaaac agggaaaaga agaagcgtgg gcagcaatcg aacaagcaat tgaatcataa
    21061 aaagaataaa aaagacttac cagtcaaact ctttcataaa agtttggctg gtaagtcttt
    21121 ttttgagctt aacttacttt tgtttcttgt cttttttctt ttcattttgt ggctgtggtt
    21181 ttagaaatcg atcgatctct tcttttggtg caactgtttt ttccggatcg acagcaaatg
    21241 attcgaaaag tttatctaaa ttatctgtac gttcaaattt caaccccatt gtaaacacct
    21301 ctttgtttct ttttacttag tctatcatgt aatcgtccaa attggtttct aagacataaa
    21361 gttttagttt ttctttatca ttggttataa ttaaattata aaaagatttg aaagaggtga
    21421 gaaaatgatt ggcgaagata aattattgat gactatccag ctcacagact taaatgaaga
    21481 agaaaaaaac aggaaataag cgattgggct attttgacac gtctgttgat ccagccaaca
    21541 tttatccgct ctttcacaca acaagcggat ccgtatgatc ttcgaaagct tcaagaaaaa
    21601 ataatgctag catcggtaag agacgaaagt tggttagttg taggaaatga cgagaacgaa
    21661 tgtagttttc gcttggaaga taatcaatta ttgataaaaa atgtattgtc tatctctgtt
    21721 ttcaaagaaa agcaattttt gattcgagac ttcattcaga aaaaaatgat tgctcatggg
    21781 gtgttcgcct atatgcgagc ttatagcgag tttatctatc acaatacaaa acagatctct
    21841 caacggctat tgtttgagaa aaaggatgaa atcgaacatt tacctaaaat gaaacaacaa
    21901 aacggggaag tagtagtcga ttgcaaccaa tttccaggat acgatctttt ttaccaaggt
    21961 ttatgtttca cttcgtgttg ggaaatgtat tatagtcgct attatcatca aatcatccct
    22021 aaaccgattt ttctcgacat ccaacaagta gaaaaagtga aggaattaga aaacgaagtc
    22081 atatgtatcc agctttatcg agaccccttt aactggcaaa agccaaacaa tcaaatgttt
    22141 caatcctatt ttagggacca gcttgggttt gatcatcttg cttgggacaa tggtgtcggt
    22201 ttattgaaac cgccattcgt tgaatacatt tacactgacc atacgatcca atctgtccag
    22261 tatcagaatg cacaaatgca acctgttcct aaaaagaatg cttcattttt tgtaacaaaa
    22321 agttatgata tccaacaagg agattataaa gaaaggcgtg ttcgcggaac actaaatgct
    22381 caggcgtatt ttccttgggt tgacgagaat cgatcacgca tgatgtgcta taaagttatc
    22441 gatccgactg ttgctttgga taatggaatc gaagcttatt gttattatat tagggaatat
    22501 ctagaagtag aggtaacaga tgaaaaatat caagagtatc ttttatcctt aagaatctat
    22561 gttccttcag aatccctgtc agaattaccg ttaaaggaaa tcaaacatag actctctgat
    22621 gttaccttca aacgaataaa gaaaaagcgg cgaagtatcc aattcgatgt caagaaaggt
    22681 gagcagcatc tgcgtgtgca atttttagat taccgagaat tagaacaatt gattacttta
    22741 caaaaaatat gaggtgaaga aaatggataa acaactctat tatcgaataa tggttccggc
    22801 atcattatta tccgtctgtc tcttttttgt tcctaatttt cctattcgat cagtcatcgg
    22861 ttttttggtc atgtttggcg ggatgggact ttattattat cttcttcata aggaaagaaa
    22921 acctgcttct gtttatatga aaaaaacaag aagcgaaaat aaaaaacgat aaaacggctt
    22981 gatgatagac aaaaaaataa aaaaccagt
//
LOCUS       DAFCNS010000045        22985 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFCNS010000045 DAFCNS010000000
VERSION     DAFCNS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.045"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            134..532
                     /locus_tag="LNE23_001705"
     CDS             134..532
                     /locus_tag="LNE23_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK6931561.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            516..1210
                     /locus_tag="LNE23_001706"
                     /pseudo
     CDS             516..1210
                     /locus_tag="LNE23_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1804..2691)
                     /locus_tag="LNE23_001707"
     CDS             complement(1804..2691)
                     /locus_tag="LNE23_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK6931562.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2876..3715
                     /locus_tag="LNE23_001708"
     CDS             2876..3715
                     /locus_tag="LNE23_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK6931563.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3911..4885
                     /gene="sgrA"
                     /locus_tag="LNE23_001709"
     CDS             3911..4885
                     /gene="sgrA"
                     /locus_tag="LNE23_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBK6931564.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5183..5539
                     /locus_tag="LNE23_001710"
     CDS             5183..5539
                     /locus_tag="LNE23_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931565.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5542..6699
                     /locus_tag="LNE23_001711"
     CDS             5542..6699
                     /locus_tag="LNE23_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6931566.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6725..7165)
                     /locus_tag="LNE23_001712"
     CDS             complement(6725..7165)
                     /locus_tag="LNE23_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK6931567.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7325..7831
                     /locus_tag="LNE23_001713"
     CDS             7325..7831
                     /locus_tag="LNE23_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6931568.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7999..9912
                     /locus_tag="LNE23_001714"
     CDS             7999..9912
                     /locus_tag="LNE23_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBK6931569.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9917..10918
                     /locus_tag="LNE23_001715"
     CDS             9917..10918
                     /locus_tag="LNE23_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6931570.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11120..11326)
                     /locus_tag="LNE23_001716"
     CDS             complement(11120..11326)
                     /locus_tag="LNE23_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBK6931571.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11972..12820)
                     /locus_tag="LNE23_001717"
     CDS             complement(11972..12820)
                     /locus_tag="LNE23_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6931572.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12819..13886
                     /gene="gnd"
                     /locus_tag="LNE23_001718"
     CDS             12819..13886
                     /gene="gnd"
                     /locus_tag="LNE23_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBK6931573.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13888..14889
                     /locus_tag="LNE23_001719"
     CDS             13888..14889
                     /locus_tag="LNE23_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBK6931574.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14886..15170
                     /locus_tag="LNE23_001720"
     CDS             14886..15170
                     /locus_tag="LNE23_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBK6931575.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15203..16000
                     /locus_tag="LNE23_001721"
     CDS             15203..16000
                     /locus_tag="LNE23_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6931576.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16014..16847
                     /locus_tag="LNE23_001722"
     CDS             16014..16847
                     /locus_tag="LNE23_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6931577.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16865..17722
                     /locus_tag="LNE23_001723"
     CDS             16865..17722
                     /locus_tag="LNE23_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6931578.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17744..18253
                     /locus_tag="LNE23_001724"
     CDS             17744..18253
                     /locus_tag="LNE23_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6931579.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18290..18691
                     /locus_tag="LNE23_001725"
     CDS             18290..18691
                     /locus_tag="LNE23_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931580.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18803..19156
                     /locus_tag="LNE23_001726"
     CDS             18803..19156
                     /locus_tag="LNE23_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931581.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19190..19633
                     /locus_tag="LNE23_001727"
     CDS             19190..19633
                     /locus_tag="LNE23_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK6931582.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19650..20735
                     /gene="uxuA"
                     /locus_tag="LNE23_001728"
     CDS             19650..20735
                     /gene="uxuA"
                     /locus_tag="LNE23_001728"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK6931583.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20728..21441
                     /locus_tag="LNE23_001729"
     CDS             20728..21441
                     /locus_tag="LNE23_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6931584.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21648..21797)
                     /locus_tag="LNE23_001730"
     CDS             complement(21648..21797)
                     /locus_tag="LNE23_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931585.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22193..22393
                     /locus_tag="LNE23_001731"
     CDS             22193..22393
                     /locus_tag="LNE23_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6931586.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7936 a         3507 c         4742 g         6800 t
ORIGIN      
        1 cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat gtgttattca
       61 ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg atattttacg
      121 aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta tagaagcaga
      181 agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg aaacattaac
      241 agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt gtgatatgaa
      301 gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg tccaaactaa
      361 tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc cggaacttcc
      421 aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga caatcattaa
      481 aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt gatacagcaa
      541 atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta acaacaaatc
      601 ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa gaaatcaaaa
      661 aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa gaagaaatga
      721 tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc aaggtgccgg
      781 tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat ttgattaccg
      841 gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga gcagattata
      901 ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa gtgattgatt
      961 cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca gctagtttca
     1021 aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa cgttaggtaa
     1081 agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga ggggcttcca
     1141 agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa aggtcgctga
     1201 ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa ctcgaggagg
     1261 ctatctgacg attcaatatt cattaattta aagtttgggt gactattact aactattact
     1321 tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa ctctgaagtt
     1381 acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa aaatatcttt
     1441 cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat ttttagtctt
     1501 ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga agcgatacgg
     1561 caagtgaaaa aatagggaag catgaaagaa tctacttaat ggcgcattga ttcatgtgat
     1621 agaaataaaa aactttttta aattatatcc tcatgattgt aacaaatcca gtgatttgtt
     1681 ttggtacaaa taaaaaaagg ccccgatgaa gttgtggcaa aagtggtttg cctcaaaaat
     1741 aagaaggaat ttccaaaaat cacttctcaa attttgccac atccccaatt gggtcaaatt
     1801 tgtttatttg aatatatttt ttacataagg atctttaata gttacattgt cttttttcat
     1861 cactttacgg attgttttat ccatgaattc cgtatcaaga gtttttgctt cggtagcaat
     1921 tttttctagc tcagatttgt atttgtcttt attagaacct ttatcctgtt tttttaccat
     1981 ttttactaga taatagcttt gctgataagt cgttggatct gttacaggaa tgatatcaga
     2041 aacttccccg ttttttagtt taaatgctgc tttttttact tcagtaggaa tctctggatt
     2101 ggttgaatca aaattcattt ttccatcagt ttcctttaat gttttatttt ttccataagc
     2161 ttgaacaagc ttggagaaat tttctccttc atttgcttct ttttttgctt tatctgcttc
     2221 ttctttagtc gaaaatagaa tgatctgtgt actaacttct ggatagaatt ctttccaagc
     2281 agtgtctaga tctttatctg ttaattttac attggctttc aaaccgtatt ggaaagccag
     2341 catctgttta aagagtttct tatatgtctc ttctgtttgg ccaactgctt ttaattgttc
     2401 cttgtagttt ggaccgagtt ttttgatttg ttcattatat tcttttgtta cttcctttga
     2461 agaaatctct tttccgtata catcatcaaa tgctttgaag acaatcattt tttttaatag
     2521 tgtttgatta tttgatttaa cttttgggtt atcataaaaa tcctgaactg taagtgttcc
     2581 acctttaaac gttgcaatcg tttcattgga cttgctacaa cctgagaata gtaaaattgc
     2641 acatgctgaa aggcacataa ttagtagttg ttttttcaaa aaatttccca tcctttcctt
     2701 attaaaaaca gtctaacaga agaattaggg agacagtctt tcattttcgt aaggttgctg
     2761 tagatattct tagttcgtaa aaatctattc tctctatgat ggagaacatt tacatagttt
     2821 tcacgagaca agaaagaaaa gggttgttaa acttaatata aaggagggat gaaagatgat
     2881 caggattatt cggttgattg gatctgtttt aatagtcttt gttggttttg ttttaggaat
     2941 tcttgtcaat aatgcacttg cggatatgtt tattggaaat agtgaatgga ttggagcaat
     3001 tgccggtacg gtcggacatt tagctgtttt tttactagct ttatttcttg ctagtaaggt
     3061 cgaaggaaaa agaatagaag attatggtgt ttctgttcga ggaaaagact gggggtattt
     3121 agcgggaggt ctcgttgttg gaattggagt gtttctgctt attactagtc cattatattt
     3181 gacgggggcg tatcggttag attcaggaaa cgcaaacatt gtgccgctga taacgagttt
     3241 tattttattt attgcagtgg gggcatcaga agagctttta ttccgtggct tttttcagca
     3301 tcagatgctt agatttggtc cgttaatagc aatgattgga tctgctgcat tatttgctct
     3361 tttgcacgga ttgaatccta acatgacatt tctggcagtt ttcaatatct ttttagcagg
     3421 ttgctttttc agcgcactta tctacagtac aggaagttta tttacggcta ttggtgctca
     3481 tattacttgg aattggacac aaggagctat tttgggtata ccagtcagcg gaacgcaaga
     3541 acaaggtttt ttctccactc tgatccaatc gcaaaataca ataattactg ggggaaactt
     3601 tggggctgaa gcatctatat caactacatt attcttgtta gtcttgattg ttggtttgtt
     3661 actctatgca tataaaacaa agcggatgaa gaaatacact gctgtttatc aataaaagat
     3721 gaagaaagca aggctgatat cggtcttgct tttttaagtc tgtcattgat taaacgaaaa
     3781 agtaaacaaa ttcaagcgaa tacacgacat cttttgttag attttcacaa ctgactgtcg
     3841 tgttctcatc attttcattt tatataattc ctttatcaaa tagaaggtaa ttatatggag
     3901 agtgatagaa atgaagaaaa cagcgaccgt attatttgtt attcttgggc tgagtataat
     3961 tgcagcaatg attggaatca gtacttctgc aaatgaacgg gcaaatgaga atctttcatt
     4021 tactgtgaaa actgatcgga ttgtttatga catgacacaa caagtcatta ctattccagt
     4081 caaaccaaac aaaagcgtga atgctagtga tgtacatgct gttctaacat atggttggga
     4141 tggtaatgga agttcagaaa aagttattgg ggaagtttat ttaaaagacg tacaatggac
     4201 cgctggtatt gagtatacaa taatgatttc tgcagaacta tcgattgatg agatcaagag
     4261 taaagataaa gttgatttga ttgtttttta tgatggacag atgacgatca cggagaattt
     4321 gaaaccaagc agttggacag ttgttggacc gccaagcagt acggatagca caagtagtga
     4381 aagcagtaca gaaaacacga gcggtgaaag cagtacagag agtacgagca gtgaaattag
     4441 cacagaaaac acgagcagcg aaagcagtac ggagagtacg agtagtgaaa gtagcacagg
     4501 aagcacgagc agcgaaagca gtacggagag tacgagtagt gaaagtagca caggaagcat
     4561 gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa aagtaaataa
     4621 tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg cagggagcaa
     4681 cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc atcaagaaaa
     4741 agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta gaaccttgaa
     4801 caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa gctattttcg
     4861 ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat tgaataaaac
     4921 gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg aagttgtagc
     4981 tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa caggaacaca
     5041 aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag tatctctgaa
     5101 aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa gtctaaataa
     5161 tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg aaaggcaagt
     5221 atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg tgaaggatat
     5281 cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac aagcagaatc
     5341 gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca agatgataaa
     5401 agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt tagagggaac
     5461 gattggttat attttactgg attcacttta caaaggtact tatcaatcct tagaaaatct
     5521 tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga acttaatact
     5581 attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa
     5641 gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga cgaaaatcat
     5701 tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat
     5761 gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag aatttcgttg
     5821 caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa caaatttgtg
     5881 catccattga taacaaaaga agagtttacg cattacatac tagagccttt ttttgaagca
     5941 aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta ttttatgttt
     6001 ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa agtacaagaa
     6061 agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt caagatttgt
     6121 tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca tttgtattca
     6181 agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga agacagagat
     6241 tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag tggggtagct
     6301 cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat ttttcaatac
     6361 tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt ctatattcaa
     6421 tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc tatgactgaa
     6481 cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat tagcgattat
     6541 cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg gaatggacaa
     6601 cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa
     6661 aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat tgtttatgcc
     6721 aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga ccagaaagca
     6781 agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg attttatctt
     6841 cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt ttcaataaag
     6901 aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct attctgtgaa
     6961 caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg ttgtttgtca
     7021 attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag ttctgttcga
     7081 taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac gagcagccag
     7141 aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt atgaaataat
     7201 aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa atgttgtcac
     7261 gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga aagaaggcac
     7321 aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag gggtatgttt
     7381 tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata aaagaatcaa
     7441 aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg ctcaattagt
     7501 gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc acagttattt
     7561 tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt tattaactga
     7621 attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta tagatacgac
     7681 tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag caacgacaac
     7741 ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga aaatttggga
     7801 taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa aaagcagaat
     7861 ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc ctgttctatt
     7921 tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca tataattctc
     7981 ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa gggagcgttg
     8041 gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg agagagcatt
     8101 tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc tggaaatgaa
     8161 acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga gacatctgtt
     8221 actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga agaagcaaca
     8281 gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa agagaatggg
     8341 acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata tttgaattac
     8401 ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa gtcaaaatta
     8461 atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa agagattatc
     8521 aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg
     8581 atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac aggcttctat
     8641 ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa aatgatggat
     8701 gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca tggcggattt
     8761 cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta taaggccggg
     8821 tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga ttatgtgacc
     8881 tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt tcgagagcgg
     8941 ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat gatgtctgta
     9001 ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg atttgcatca
     9061 attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact accggatgcc
     9121 ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt tatgggcctt
     9181 ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc tctacctacg
     9241 attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga tgatgtagta
     9301 cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga tcttttatta
     9361 gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta cacgaaacat
     9421 gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta tgtggatgaa
     9481 aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc ttatggagaa
     9541 aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg
     9601 actgaaggga tttttaatga gacgatacca actattattt tctattacaa aaaaatccca
     9661 gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg aaagaatgaa
     9721 cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt gaaagtacaa
     9781 aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa acattcgctt
     9841 ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat ttggcgaaga
     9901 aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac cagttatcat
     9961 cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc cgattagctt
    10021 gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc gaccagatga
    10081 tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc cttcaaagta
    10141 caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat taccgattct
    10201 tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc gtgattattt
    10261 ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg aacgatcacc
    10321 ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag cagctaaaaa
    10381 agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc
    10441 acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa aagctgatca
    10501 tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt ctggtctgtt
    10561 ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt atgaaaaatg
    10621 tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca aaagtttagc
    10681 cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg gaaatacaaa
    10741 tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt
    10801 cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc agtttgattt
    10861 cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca aggagtaaag
    10921 tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa ggagcaattt
    10981 caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg agttagagat
    11041 ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt cacgatctct
    11101 tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc
    11161 cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag aatgctggtt
    11221 ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata gcaaataaaa
    11281 tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc agcacctcct
    11341 tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt ttgttaggat
    11401 gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata agcaattaat
    11461 gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa taatagaaaa
    11521 tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc aaaaattcga
    11581 tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat aaaattacaa
    11641 atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta attcaccaaa
    11701 atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga aaagataaat
    11761 tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa
    11821 tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg
    11881 taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt
    11941 ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc
    12001 gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata
    12061 gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg
    12121 tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt
    12181 ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg
    12241 tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag
    12301 aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca
    12361 aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga
    12421 cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact
    12481 tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct
    12541 cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga
    12601 tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa
    12661 aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta
    12721 ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt
    12781 tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata
    12841 ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga gtaggaaaaa
    12901 atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg
    12961 gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat
    13021 tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa
    13081 gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat
    13141 ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa
    13201 ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag
    13261 gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa
    13321 tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct
    13381 taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag
    13441 caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga
    13501 tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac
    13561 aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg
    13621 tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa
    13681 gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc
    13741 tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac
    13801 aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac
    13861 atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc
    13921 agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt
    13981 catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga
    14041 ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa
    14101 gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca
    14161 gcaaaagaac accagacaat cgtatctatc atacctgctt taattgaacg aaatgctgta
    14221 gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca
    14281 agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac
    14341 aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt
    14401 aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa
    14461 agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt
    14521 ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag
    14581 atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg
    14641 atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa
    14701 gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat
    14761 atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc
    14821 gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata
    14881 aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt
    14941 tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa
    15001 gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt
    15061 tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg
    15121 aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg
    15181 cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg
    15241 agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa
    15301 ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat
    15361 aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga
    15421 tacactagca aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga
    15481 atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca
    15541 ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct
    15601 ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag
    15661 aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg
    15721 ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg
    15781 ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct
    15841 agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat
    15901 tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca
    15961 gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa
    16021 cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct
    16081 ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg
    16141 tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga
    16201 tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag
    16261 ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg
    16321 cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt
    16381 ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat
    16441 caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg
    16501 ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg
    16561 ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg
    16621 tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg
    16681 ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg attattatcg
    16741 ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca
    16801 attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg
    16861 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
    16921 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
    16981 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
    17041 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
    17101 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
    17161 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
    17221 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
    17281 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
    17341 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
    17401 cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga
    17461 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
    17521 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
    17581 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
    17641 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
    17701 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
    17761 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
    17821 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
    17881 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
    17941 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
    18001 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
    18061 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
    18121 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
    18181 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
    18241 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
    18301 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
    18361 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
    18421 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
    18481 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
    18541 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
    18601 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
    18661 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
    18721 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
    18781 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
    18841 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
    18901 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
    18961 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
    19021 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
    19081 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
    19141 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
    19201 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
    19261 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
    19321 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
    19381 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
    19441 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
    19501 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
    19561 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
    19621 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
    19681 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
    19741 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
    19801 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
    19861 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
    19921 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
    19981 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
    20041 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
    20101 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
    20161 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
    20221 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
    20281 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
    20341 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
    20401 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
    20461 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
    20521 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
    20581 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
    20641 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
    20701 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
    20761 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
    20821 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
    20881 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
    20941 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
    21001 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
    21061 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
    21121 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
    21181 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
    21241 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
    21301 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
    21361 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
    21421 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
    21481 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
    21541 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
    21601 cgtttaaaga gaagaacgag aatcggccat atgagggttt ccactcctta tgcagatttt
    21661 tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct
    21721 ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct
    21781 ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac
    21841 gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa
    21901 gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat
    21961 caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat
    22021 atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta
    22081 ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg cctatcaaat
    22141 atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa
    22201 cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg
    22261 aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga
    22321 aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat
    22381 tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt
    22441 tgtacgaaaa aattcattgg tcgttgaatg ggctgaatag tagtgtataa aacaattttg
    22501 tggagcgtga agcttttatg gtagaggaaa atacgaataa agtgatgttg ataaaaagat
    22561 tcctatcttg aatatgaatc gagatgtctt gaaaatgaat gagtttgacc aatcattatc
    22621 ctatcgaaat ttacagtttc gttcgattgg tctgtcaatt aatgaaaaag taaaaaagaa
    22681 tatgttctag gaagagggcg gcacatctaa atttttaagt aacggacagt tcaaatatca
    22741 agcattgaaa gaggctatcc tactgtttga aggaatacga atatataaaa acgacaaaga
    22801 ggaaaaagta gttcattaaa tcccttcaag tgatgaaaga atgagatcta ataaaacctg
    22861 atctttcatc tgttttttta ataacgtaat aattttttta ttttttgtgc tttcgccgtt
    22921 attgtagagt gatttagcat tcttattttt aaaaataact gtgttatata ttaaaaaagg
    22981 cataa
//
LOCUS       DAFCNS010000046        22713 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFCNS010000046 DAFCNS010000000
VERSION     DAFCNS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.046"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            21..257
                     /locus_tag="LNE23_001732"
                     /pseudo
     CDS             21..257
                     /locus_tag="LNE23_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            472..1224
                     /locus_tag="LNE23_001733"
     CDS             472..1224
                     /locus_tag="LNE23_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6931587.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(1840..3282)
                     /locus_tag="LNE23_001734"
     CDS             complement(1840..3282)
                     /locus_tag="LNE23_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HBK6931588.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNRII"
     gene            complement(3304..3606)
                     /locus_tag="LNE23_001735"
     CDS             complement(3304..3606)
                     /locus_tag="LNE23_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBK6931589.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            complement(3663..4142)
                     /locus_tag="LNE23_001736"
     CDS             complement(3663..4142)
                     /locus_tag="LNE23_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6931590.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            4307..5080
                     /locus_tag="LNE23_001737"
     CDS             4307..5080
                     /locus_tag="LNE23_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6931591.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            5100..5528
                     /gene="lacA"
                     /locus_tag="LNE23_001738"
     CDS             5100..5528
                     /gene="lacA"
                     /locus_tag="LNE23_001738"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HBK6931592.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTREHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            5547..6062
                     /gene="lacB"
                     /locus_tag="LNE23_001739"
     CDS             5547..6062
                     /gene="lacB"
                     /locus_tag="LNE23_001739"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HBK6931593.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            6075..7001
                     /gene="lacC"
                     /locus_tag="LNE23_001740"
     CDS             6075..7001
                     /gene="lacC"
                     /locus_tag="LNE23_001740"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HBK6931594.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            7014..8000
                     /gene="lacD"
                     /locus_tag="LNE23_001741"
     CDS             7014..8000
                     /gene="lacD"
                     /locus_tag="LNE23_001741"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBK6931595.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            8456..9124
                     /locus_tag="LNE23_001742"
     CDS             8456..9124
                     /locus_tag="LNE23_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6931596.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            9115..10248
                     /locus_tag="LNE23_001743"
     CDS             9115..10248
                     /locus_tag="LNE23_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6931597.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            10707..11279
                     /locus_tag="LNE23_001744"
     CDS             10707..11279
                     /locus_tag="LNE23_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBK6931598.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            11424..12128
                     /locus_tag="LNE23_001745"
     CDS             11424..12128
                     /locus_tag="LNE23_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK6931599.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            12357..12695
                     /locus_tag="LNE23_001746"
     CDS             12357..12695
                     /locus_tag="LNE23_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931600.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            12680..13534
                     /locus_tag="LNE23_001747"
     CDS             12680..13534
                     /locus_tag="LNE23_001747"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBK6931601.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            13681..13914
                     /locus_tag="LNE23_001748"
     CDS             13681..13914
                     /locus_tag="LNE23_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBK6931602.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            14112..16016
                     /locus_tag="LNE23_001749"
     CDS             14112..16016
                     /locus_tag="LNE23_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK6931603.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            16326..16688
                     /locus_tag="LNE23_001750"
     CDS             16326..16688
                     /locus_tag="LNE23_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6931604.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            16705..17316
                     /locus_tag="LNE23_001751"
     CDS             16705..17316
                     /locus_tag="LNE23_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HBK6931605.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(17418..18326)
                     /locus_tag="LNE23_001752"
     CDS             complement(17418..18326)
                     /locus_tag="LNE23_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK6931606.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(18368..18682)
                     /locus_tag="LNE23_001753"
     CDS             complement(18368..18682)
                     /locus_tag="LNE23_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK6931607.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            18981..20912
                     /locus_tag="LNE23_001754"
     CDS             18981..20912
                     /locus_tag="LNE23_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK6931608.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            21205..21756
                     /locus_tag="LNE23_001755"
     CDS             21205..21756
                     /locus_tag="LNE23_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6931609.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            22069..22611
                     /locus_tag="LNE23_001756"
     CDS             22069..22611
                     /locus_tag="LNE23_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931610.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         7394 a         3819 c         4853 g         6647 t
ORIGIN      
        1 gcttaatttg acaaatcaga aaagaaaaaa tgtatcaagt ttttgaagaa atgggttatc
       61 caattaaaac ggtcaccaga aaactagaag taatcaaagc ggatgatacg atcagcgatt
      121 tgttagatgt ggaagaagga gatccaatgt tctatttcca tacgcagggt ttttcgctag
      181 aacgtctgcc cgttgagtat tcgatttcga aatatcgagg agacattaac tactttgttt
      241 ttgaacttgc acaataactt cagattaaat aaaatgattt attaaagata aaagtcgaat
      301 gaacaaggct gtcactatga cagtcatgct cgttcggttt tattttttag aatagcagct
      361 tgcaaattct gaaaaatttt gctttccttc ctgcagaagg cttcttaaga aaacttgttc
      421 tagcactttt cttaagaatt tgatagtctt aaaaagaaaa gaggcgatga aatgttaaaa
      481 acggatcgat tccaagcgat catgtcgtta ttagaggtta acggtacagt acgagtcagc
      541 gatattatgg agaagttttc ggtttcggat atgacagtcc gacgtgattt ggacgaaatg
      601 gagaaacaag gattattaga acgcgtccat ggcggagcga aattgaagga tttttatcgg
      661 catgtggagc tttctcatag agaaaaacag attatgctgc aagaagaaaa gcagcggatt
      721 gtggaaaagg cgaatgaaat gatcaatgat ggtgatacga tatttcttgg accaggaact
      781 acaatcgagc tcatgtcaaa aatcattcaa ggggattcac tgcggattgt tacaaactgt
      841 ttgcctgttt ttgaagaatt aaataagcga aaagggaatc gaaaaatcta tctattaggt
      901 ggcgagatgc gggaaacgac tcaatgcttt gttggtgaaa tcacgaataa atcgctacag
      961 gatatgaatt ttcataaagc atttttcagc tgtaatgctt taaatgaaca gaaaattatg
     1021 acttcgactt ttgaagaggg ccaaactcaa cagatcgctc tgaataattc tgttgaacgc
     1081 tttttattga ttgatcattc gaaaattggg aaaagagatt tttcagtata ttataatctg
     1141 aatgatgtaa cagtagttat aacggatgat gatgaacaaa agcactatga aaaaatcgaa
     1201 aaagaagaaa aatatgttgt gtaaatgaga aactgccaaa ctctagataa ttagaacttg
     1261 gtagtttttt ggtagccttg aatttaagcg aattatgtta tttttagggt gcatcattac
     1321 taattcctcc gaatacttga atacaccatt tgaaaaaaga tagtcgattt ttttgggatg
     1381 ttatgtttat gtgaaggaaa aaatagcagt atcaatcacc gctttattaa ggaggaaaag
     1441 ggatatcaaa acaaccatgg acactaagtc agctgaagct ctttgacatg aaacgagtta
     1501 gatcagaaaa agcaatgatg aaatcgcacg cataaccgcg ggatgcgaag cgattcagga
     1561 aaaaagaaat caaggaattc gcaattaaac aacaaaaagt caaaaagcac aaaacaaaca
     1621 agaacacaaa aagaatcgtt gaggaatacc aacaaaggca agcttaaagg agttaaaaaa
     1681 agcataaacg ttgataattt ctttattggg tgtggcaaga gatgggctat gctttttgaa
     1741 atggatttta catctattta ttttttatag gtaaaataat accccgaagt ctaaagacat
     1801 tcggggtgtt tttagcttat ttagcttcgg ctgcataagt catattatgc gattatgcgc
     1861 cgttgccgga gatgcattct cttctttttc agctgcgtat gcagcgttgc gttttttcat
     1921 ttgacgagcg taccaaatga agattaaaag gtatgcacct aatgctaagg cagcgtatat
     1981 cacaaattcg atttgtcctt gagaagcatt accgactaaa taacctagga atttttcaat
     2041 cgggccttcc atcgttgtat gagagatcat ggcggacccg ttcaagccgg ctggaaaggc
     2101 tccgactgct ttagctgttt gagtaacgaa cggggcgatc aaggtgccgg accataagaa
     2161 taatggtaat tcgatcgcgc cgattacgat catacggatt aattttccac gagttacgac
     2221 caatagtgct ggggtaacac ccattgcgat gattccgcct agtggcaata atttatttcc
     2281 tggtaagatg atggcttcta gcaacatgat tggtgctaat acgttagcgg cagcccagat
     2341 ttcagcgcgg cctgctatga aaggccagtc taaacctatg ttgatggtac gtccttttag
     2401 tcgtttattg gcaaaatcgg taatcccttg tgataacggt tctacggctg cgatgaacca
     2461 agcgcctatc aaagagaaca attccaaaca gacggcagcg gtaaaggcta gcgagaaaat
     2521 ttgcgttggt gtttgtcctg ctagtaatcc aacaaaaatc cccaaataaa ttccaatcgc
     2581 gaattttgat ccccagaagc caattttact attcaatttg gcagcatcga aatcatattt
     2641 atccaaccaa gggaagattt tgtcgaaaat tttatcgaaa accataataa tcggattcat
     2701 catgtagttc atatgagtcg tggtcatcgg gttgctgtct ggcgcattta acaagtcatt
     2761 gaaggttggt ttcattaagt cggagttcca aatttttaat acaccaatga aaataactaa
     2821 tgcagaagca ataaacagat tagcaccgca gaagatcgcg aacaaaccaa caattgctaa
     2881 atgccaaata tcgaaaatat ccacatctaa tgtgttggtt tttttcattg ctaacatgac
     2941 aatattgacg atcaccataa ttagtaaaaa ataaagcgtg tatggcgacc cccatgtgat
     3001 ggttgctagt ggggcccaac cgacatctgt aatgtttaat gaaatacctg tgcgttcaac
     3061 aaaagctgtc atagcgtctg aaaaagcagt agttaaaatt ccaataatag ctccgatccc
     3121 agtcagggcg atccctaatt tgagcccgcc ttcaagagcg cgtgacggtt ttactttaaa
     3181 aaataaagca ataacagtta atacgatggt cattaacggt gccgcaccta aattaattag
     3241 gggggtaaat actttattgg caatatcaat aattgtattc atatgctttt cctttcttta
     3301 gtcctatttg tttaatgatt ggattgcgct ttccatactt tggtaaacag gctcagccat
     3361 agcaggaata cgataaagaa tagcacctgc atcaacgaca gggatgttaa tgtcaaaacc
     3421 caagtcggtg ttggcaattt gtgcgaaaat gtcataatga gacagcattt cttcattgat
     3481 atctttgatc ataacggcgt cacaggtgac ttgatagcca cgatttttca attcagtttc
     3541 caaagcgctt ttgatttgat ggcttgaatt aacgcctgct ccacatgctg ctaaaatttt
     3601 gatcataata attctcccct taaaataagt ttatttttta tcctgcggtt aggcagagac
     3661 ttctatatat aaaaatgttc ttctaaaaaa tgataaatcg cttctgtgtc ctctaatttg
     3721 aaaaacgaaa acagtttttc ttcgtcaaca gaattaatga aatccatgat attagccaac
     3781 atgccagcct gtgcttcacc attttcgttc aagatcatga aaagaaatga agcggaaatc
     3841 tgatcgcttg gcgagatcat gttgtagaaa gggatttcat tttttacttt gattggaatg
     3901 atacgggtgg ttcgcacaaa ctcactttcg gtatgaggga tagcgatatc tggcaatatt
     3961 ttagagacag gtgagagatc taatcccgtc ggataattac gctcccgttc taaaaggttc
     4021 tccaaaaaat cttcggttac caattctttc tttaacagat cttgatagac ttcctcgaac
     4081 acttcttcct gcgactgctt gtcagaaaca tagacagtgt ctttgaaaaa gaattcattc
     4141 acacaaaaca gctcctttat tagttcgttt atgtttattt atctgtttac aagttgagta
     4201 taaccgctta caaaattatt gtcaacgcat aaacgaaaat aaacgaacaa tatgtgttga
     4261 aaaatgttcg taaatgtttt attataaggt cgtggaggta cagaatatgc tgaaaacaga
     4321 acgacagcgt tcgatcttag cgatgtgtga tcgctatggg acgataacag tcaaatttat
     4381 tcaggaagta ctgaatgttt cagatatgac gatccgccga gatctagagg aactcgcgca
     4441 gaagaaaaag cttttacgcg ttcatggcgg tgcacaaaaa atcgattatc tggaaatgtc
     4501 gacttctata gaagcaaatg agctttctca tactgaaaaa aagaagctgc atacaaaaga
     4561 gaaggaatac atcgctaaaa atgcggcagc tcatatcaat caagaagacg acacgatttt
     4621 tcttggatcg ggtacaacga tcgaattaat gacacgatac atcaagccgt catctctccg
     4681 aatcgttacc aatagtctgc cggttttcaa tctattaagt gaaataaaaa gatttgaact
     4741 gtatttgatc ggtgggctat atcgggaaaa gactggagcc tttgtcggtt caattgccaa
     4801 tgaagctgtt caaaaaatcg gtattaagaa ggcttttgtc ggagtgaatg gattgaaggg
     4861 ttcggaggtt tcgaatttca gtattgaaga aggtaccttg caaaaaattg ttttagataa
     4921 tgcggcagaa aaatgtttgg tttcagatgc aaacaaattc gaccatagcg atttttataa
     4981 tttctatgat ttgcgcaaga tcgacaaatt atttacggat tctactcttt cagccgagct
     5041 gaagagttat tatcagcaat tcactaaaat cattcagtaa aaaataggag gaaacaaaaa
     5101 tgcgtgtagt aattggttca gacagtgacg gcatggaatt aaaggaatta atcaagaaaa
     5161 aattgatcaa agaaggtatt acagtagtag ataagagtga aacggcttca gaagattttg
     5221 tggaatcaac tttggcgatc gcaaatgatc tgaatgaaaa cgaaggtagt ttaggaatcg
     5281 cggttgatgg ctacggcgct ggcagcttta tcacggcaac aaaggttaaa ggaatggtag
     5341 cagcagaagt ttcggatgag cgcagtgcgt atatgacccg tgaacataat aattctcgga
     5401 tgatcacact tggatcaaaa attgttggcg atgagttggc gttgaatatt gttaaggaat
     5461 ttctacaagc aaattatgat ggcggtcgtc atcaagtacg tgtcgacatg ttaaataaaa
     5521 tggcgtaatt aggaaggatg aatgaaatga aaatcgcaat tggctgtgac cacatcgtca
     5581 cagatacaaa aatggcagta tcagatttct taaaatcaaa aggacatgaa gtgttggata
     5641 tggggaccta tgactttacc agaacccatt atccgatcta cggaaaaaaa gtcggagagg
     5701 ccgtgactag cggagaagca gatctaggag tttgtttgtg tggaacaggt gttgggatta
     5761 acaacgctgt caataaagta cctggtatcc gcagtgcgct ggttcgcgat atgacgactg
     5821 ctatctatgc gaaggaacaa ctaaatgcca atgtgatcgg ctttggcgct cggattacgg
     5881 gagaattttt gatctgtgac atattagagg ccttcatcaa tgccgattat caagaaactg
     5941 aagaaaacaa aaaattgatc gccaaaatcg atcacgtcga aaaagccagt gacaagcagt
     6001 ctgatccgca tttcttcgat gaatttatcg agaaatggga tcgtggcgaa taccacgatt
     6061 aagcgggtgg acaaatgatt ttaacaataa cgatgaatcc atcgattgat atttcttatc
     6121 cattagaaac cttcaagctc gacacagtca atcgagtaag aaatgttcgc aaaacagctg
     6181 gcggtaaggg cctaaatgtg acacgtatct tgaagcaatt agatacggag gtgattgcta
     6241 gtggtttaat cggcggtttt ttaggtgaag agattaaaaa ggacctgact caaaatgaga
     6301 ttgctcatga ctttttacct atcagcggag agacacgtaa ttgcatcgct gttttacatg
     6361 acggaaaaca gacggaaatc ctagaaaacg gacctgttat tactgaacaa gaagcaaggg
     6421 cgttcttgca acatttcgaa aaattagctg ttaaagccga aatcatttct ttttcaggaa
     6481 gcttgccaga aggattgcct gctgattttt atcgacaaat gctgcaagtt tgtcagccaa
     6541 agcctgtcgt tttagattgt tcaggtacag cattaaggga agtgttgcga ggcagtgtaa
     6601 agcctaagct aataaaaccc aataccgaag agctggctgg tctgctagga cgagaaatct
     6661 caacagaatt atcagattta aaagccgcct tgtcagattc gatgtttgat gaaattgaat
     6721 ggatagtggt ttcaatgggg ggcgacggag cctttgccaa acatcacgag actttttatt
     6781 gcgtgacaat tccaaagatt gatgtcgtca atccagttgg ctctggtgat gcgaccgttg
     6841 ccggattgac cgctgcgatt caacaaggtc tttcggatga agccgtgtta aaacagggta
     6901 atgttttagg aatgctgaat gctcaggaag cgacgactgg ctatgtccaa ttagccaatt
     6961 atcaaaaact atatgatcaa atcttagtgg agaaagtgta ggaggatacg gaaatgaacg
     7021 aagaaaaacg tcaatatatc aagcaactat gcaatgaaga tggagtgatt ggggccttag
     7081 caattgatca acgcggggct ttgaagaaga tgatcacaaa attcaagggc agcgaagcag
     7141 aagaatcaga aatcgttgaa tttaaagaaa ttgtttcttc acaattaacg aaatatgcct
     7201 catcgatttt attagatccc gaatatggct tgccagcagc tgacgttaga gcaaaaaatg
     7261 ccggcttgct gttagcttat gaaaaaactg gttacgatgc gtcaactccc ggccgtttac
     7321 cagatttatt atcgatctgg tcagttaaac gtttaaaaga agcaggcgcc gatgcttgta
     7381 agttcctact ttactatgat gtcgatgaag cagaagaaat caataatcaa aaacatgcct
     7441 ttattgaacg aatcggctca gaatgtcagg ctgaaaactt acccttcttc ttggagcttg
     7501 tttcttatga cgcgaacatc tctgatgcga cttcgaaaga atacgccatt aagaaacctc
     7561 ataaagtcaa cgatatgatg aaggaatttt ctgatccacg ttatggcgta gatgtgttaa
     7621 aggtggaagt acccgtcaat atgaattatg tggaaggcta tgggacagac gaagtcgtgt
     7681 acacaaaggc acaagcggct aacttcttca aggaacaggc tgaagccact aacctgccat
     7741 ttattttcct aagcgccggt gtaaaagcag agttgttcca agaaacactg cgcttcgcga
     7801 aaaatgccgg ctcaaccttc aatggtgtac tttgcggccg tgcgacttgg gcagatggtg
     7861 tagaaagcta tatcgttgat ggaaaagaag cagcgatgga gtggatgaat acgcaagggc
     7921 gtaaaaatat tgaagaatta aatgccgtat tgaaagaaac tgctacaccg gtggaagtgt
     7981 taacaagctc actatcataa gaaatcagac tgtgaagcac aattgcttgc agtctttttt
     8041 tatacataaa gcgtaaaatc atgctgcatt attaggggaa cgaattctaa aatagtaagt
     8101 gagttaaaac gaaggggttt ttctttagag agattttctt acatgtggta cttaatgact
     8161 tgatggcaat tttcagtatt ctaacgaggc ttattggtca taaaccaacg aactttgaaa
     8221 agtatataca agaaaaccaa gcaaatagaa agtagtgaga ctggaggaaa ggttccagtc
     8281 tttttctatt ctaggtattt tcaaacagta aaatcattct taattatgtg ttttttaaca
     8341 gaatgtatga atttcttcaa atcttcacaa aaagaacgtg gactttgaag aagagcgtgt
     8401 actatgctaa aaagcataag tgtaattact aaatccgtat gaggagtgaa tatacatgcg
     8461 tgtactggtg gttgaggaca atcgtgagct ggttttatcc gtaaaaaagg gcctagagca
     8521 ggagaatatt tctgtagatg tagcttatga tggcgagtca ggagaagaga aggccttcat
     8581 caattcatac gatgggatac tactagattt aaatctgcct aaaaaagatg gattatctgt
     8641 tttacaaagt ctccgtgctg aagaagtcga aagtccagtt atcattatta ctgcacgtga
     8701 tgagattgaa gaacgagcaa aaggcttgga ttttggagca gatgattatt tagtcaaacc
     8761 ctttgagtta ctagaattaa aagcacgaat acgcgcggtt attcgtcgtt atcatggacg
     8821 tgcgctgccg cagatttcaa ttggtgcttt gaagattgat ccattaggtc gaagggtcag
     8881 ctatcaaaag acagaggtgg tcctgaaacc caaggaattt gatatcttac tgtgcatcgc
     8941 tcagcgtcat ccagcggttg tttcgtctga ggaaatttct gaacatgtct acgatgaaga
     9001 ttttgatcct ttttcgtcgg ttctgcgagt gcatctggct cgtctaaaaa agaaactggc
     9061 tgaagcagct gaaaaagagg tcttaaaaac cattcgagca aaggggtacc agctatgcta
     9121 ttaaatttta aaatgacgtt aacgatgctg gctttttcac tgttggtgac aggcgtgatt
     9181 ttagtgagtt tcttctttct gagaaataat ttggagggaa tcagtatcgc atctacctta
     9241 ggtgaagatg tttattttat ggaagagggt aagacggttc aagcagagac gcagatggaa
     9301 gcttcgaaat cttccaacaa tcaatttatg actatttcga tgatagataa agcttaccag
     9361 gacgaactat ttaaatattt accagcgatg gtgctggtta tttgttctgc cgtcatgtta
     9421 ttaacgttta ttctttggtt ggttttacgc aaaatttaca gtaatcaaat cttagcgatt
     9481 acgcgtgatt taacgtttat tgatcaagtt cctaaaaata ctgtgaagga cgaggcatta
     9541 aatgcggctt ttaataagct aaaggataag ttcgatggtc atttggagga ttatcgacga
     9601 ttaaatagtt atctgaccca cgagcagaaa aatgccatcg ctattttgcg tacgaatctg
     9661 gaaatagaac ggcaacatgc cgatcaagga aatttaatgc ttttagatcg gatgaccgac
     9721 agtattgacg atattttgac attatccaat aactcagagg atcttagcac ggaagtcgta
     9781 gacgtttcac tgatctgtgc ggaagtttgt gatgcctaca aaatgaatta tccgaatctt
     9841 ttgtttgcgt ttactgaaga tcaaccaacc ctgattcatg gacgagatcg ttggatttat
     9901 cgggcggttt cgaatttagt tgacaatgca atcaaatatg gtgaaggaaa actggtaatt
     9961 gtcaccattt caaaccaaaa gggtagcgtg attattgaag taaaagatca gggaattggc
    10021 attcctcagg agaaacaggc atcaattttt tctcatcagt accgagtgca tgggctcaag
    10081 caggatggct tacctaaaac aatggacttt agggacacgg atgaaacctt tattcaatcc
    10141 gttgcctcga aaagaaacca tattccaaga aaatcattaa attatcgaac accattggaa
    10201 gtatttttga gctgcataga taaagacata ttgtctagct taatttgaca aatcagaaaa
    10261 taaaaaaatt cttttcaatt tttatttatg gtgtgacata aataaagtaa agttattctt
    10321 ctaggttaat cttattaatg ctctttctca ctagtgttgc atgcaacact agtgagaaag
    10381 agtattaaat atactgcaag gtaagacatt tggtaattga agaataacaa gcaaaaaaat
    10441 agcagtcagg tctcttgaca aagtatagtt gctattcttt ttttaggccc tactttaatg
    10501 aaatttttgt ttggagggga cgacttcatt cctttgatta tttcagttga atttaataga
    10561 gaagcctggc gtctattaag aaatactttt cttgatgagc gtcaattttt ttaacggaaa
    10621 tctatcctat actaaattca gtaaagaaaa aggaggaatc aaagaaagct attttaaaca
    10681 aaccaaaatt ataggaggaa taaatcatga ctgaaaataa agctactcaa gaaagaacac
    10741 cccaagaaag attgcaagaa gaacaagaac acaaggaaca tgttcatcat acgaaaatta
    10801 atgcagcggc cattgcggat catcttttag gcaacatgca tacattacat gtgaaactgc
    10861 accaatatca ctggtatgta aaaggagcca atttctttac gttgcatgaa aaatttgaag
    10921 agttgtataa cgaaaatgaa aaatggttcg acaaacttgc ggagcgtcta attacttctg
    10981 ggcataaacc tgcttcgaca acggttgaat ttgaaaaata ttcgatgctt tcggaagatg
    11041 cagttaataa atacgctaaa gcagaagaca tggttgaaaa tattatcgag gacttcagaa
    11101 gcactcgtga cctaactatt cgcgcgattc gtttagctca agacgagggt gacgatgctt
    11161 tagaagatac attgattgct tttaaaaatg acttagacaa gaacatttgg atgctgcaag
    11221 cgttcattgg taaagaagca ttagaagatg atgacgctct tgatgaggat gaagattaag
    11281 gtaaaagtag aagctgattt agggattgga attctttcca gtcctttttc cattttcttg
    11341 atgtgcatca atttttttgt agtaagagac ttatataata aaagcagcga atataaacaa
    11401 aataaataaa gggggagaat gaattgacta aacataccca tcatgaaatt ggcgaccacg
    11461 cagcctgtat ccgcttggtt ccaatattca atcacttaga ggatagcgcg atgaattaca
    11521 ttgctgaaaa agcccacacg aaacagtatc aaaaaggaga gtttctgttt cgttcacaag
    11581 aaaaagagga tactttatat attatcaatc gtgggaaaat ccgtatttat cgattggcag
    11641 actctggcaa agagcagcta gtgcggatct tgaaccctgg tgatttcacc ggagaatgga
    11701 ccttgttcaa cccggatgca gtacacgagg aatatgccga agcaacccga gataccgttg
    11761 tctgtatgat tcagcaaaaa gatgtccaag attttctgga acaataccca gctatttctt
    11821 taaaactatt ggcggaaatg tctaaacggt tagaaagttc agaacgtcaa acgacacaag
    11881 tagccgtgga gagtgtcatt acccgtttag ttttgttttt ggcagaaaat gtggaacctg
    11941 aaatgggcaa ctctcccacc atcaccctgc cgatggcaaa aaaggacatt gcttcctatt
    12001 taggaacgac acctgagacc atcagtcgca aatttggaga attggaggac gagggattga
    12061 ttcaacagct gtctgggaaa aggatcaaga ttcaggattt agatgattta ttgctttaca
    12121 gcgaataatc ggacacactt ttttctacac gttggtgttc tgtcgctctt cattaacaac
    12181 gggacagaac tcagtgcttt tgcttccatc tgtccgtttt tgactttttt aagacagaac
    12241 aaccttcatt cattggaaaa ataaaagcac aaacatgata accctcaagt ttttttgttt
    12301 tatttttctt taagataggg ttgtcgaaca aaacgtaaaa ataagtaggg gtgaatgtgc
    12361 attgtttttg ggtgatagtt gccattacgt tgtcagcagc gggtgcattc tttgtcgtcc
    12421 ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt gaggcagata
    12481 ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc ggacacggaa
    12541 aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact gtttggtttc
    12601 catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg cgaaaaattg
    12661 aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc atgtggtgga
    12721 gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca tattagcggc
    12781 tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata ccattatcga
    12841 tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt atgtgttatt
    12901 tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga atggcggcat
    12961 tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg caaagaaaaa
    13021 aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt tagcccagtc
    13081 cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg ttactcgtca
    13141 attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata tgaatggtac
    13201 ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct ttgtcctcat
    13261 cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt tgagttacgt
    13321 tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca gtttatggat
    13381 tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg gcgcgattgt
    13441 agtatggttt atacgcagac aagattatcc accaatttct tattattctg atggcaataa
    13501 ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag aagtgcaaaa
    13561 cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg taaagaggaa
    13621 aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga gataggaatt
    13681 atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat gcaaaaaatt
    13741 gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt attattcaac
    13801 tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga gattcaaaaa
    13861 gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt ttaacagatt
    13921 aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt ttttgttgca
    13981 atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga tgtcagtcaa
    14041 ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa ttaaatttgg
    14101 agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc gtcatcagtg
    14161 gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta ggactttcag
    14221 agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt caagcgtttc
    14281 aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca gcgattggtg
    14341 cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc ttattcggac
    14401 actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa ttgactgaaa
    14461 tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa gtagaagtgg
    14521 atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt ccagtagatg
    14581 gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt gaagctattc
    14641 cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa aacggaacga
    14701 ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt attgaactcg
    14761 tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc ttttctaaat
    14821 actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt caaaatattg
    14881 agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc ggagtgcctg
    14941 tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta aaagggagtg
    15001 aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca ggtactttaa
    15061 ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct gctgaaacgc
    15121 ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa gcggtcttaa
    15181 atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg aaaggcggcg
    15241 gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc ttgatggaaa
    15301 aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt gaacaacaag
    15361 ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg ggcattcgcg
    15421 atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg ggcgtgaaaa
    15481 atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc ggcgaacttg
    15541 gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat atcggaaaac
    15601 ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat agtccgtcct
    15661 tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg attgaaacat
    15721 ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta ggattagtaa
    15781 aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta gtgttggtct
    15841 tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg ttggttcatg
    15901 aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat aagttgaaag
    15961 gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata tcttaagaat
    16021 aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg aaactcagat
    16081 ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga gtatagagta
    16141 tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa atttaataat aaaatttaaa
    16201 ttcctattaa gttgtaacac atgaaattgc ttataatagt gtattgaaat taaggtagat
    16261 tgacaaacga gtgatcaaat agatatacta ccttcaaacg accgtttgaa tgtgaggtga
    16321 gatgaatgga aaaagtttgc caagtgacta tcgtacataa agaaaaagta aatgtaataa
    16381 aaaaaaaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa tgtttcagcg
    16441 attcttctag gattaaaatt ttctatgctt tagaaaccta taaggaaatg tgtgtctgtg
    16501 atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta cgttttttaa
    16561 aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattat tctttagcga
    16621 atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat ctatctatga
    16681 aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat cgctgtttat
    16741 atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca aagccacaca
    16801 aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat tttggtagcc
    16861 gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat cattggtctt
    16921 ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga agaggaggaa
    16981 gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt attctggaca
    17041 gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat tccatacttt
    17101 acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat ctctgttgcg
    17161 gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga aacgttagaa
    17221 aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt tattatgata
    17281 gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg aaagataaaa
    17341 agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa tgaagtattt
    17401 tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc ttctttgctt
    17461 gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac ttctgcaatt
    17521 tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac cgtgatgata
    17581 agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc ttttacagca
    17641 ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg cgaaccattc
    17701 aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat ataaagtgca
    17761 atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat tagaatactc
    17821 acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt caacatgctc
    17881 tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa tgcaatagct
    17941 acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat caaacgtggc
    18001 acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt tatcagcgcg
    18061 cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt tttctctgaa
    18121 taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc atgaactgcg
    18181 tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct tatagagaaa
    18241 ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg attcgagtga
    18301 ttatggtctt tatctgattt tgacatttaa aaacctccta agtttgtatg ctacatacta
    18361 attggtttta tttttctaat tcattaacat gagttaagac ttgttcgaga atactaaaaa
    18421 catgaaggtc atctaaactg taaagcatac tttttccttc tcttcttgat ttcactaagc
    18481 gtgcgtcttt caacgttttt aattgatgag aaatagcgga ctgttccatg tccagagaaa
    18541 tagcgatatt tccaacacta cactcttttt tctgtaaaag aaagagaacc gacaatcgtg
    18601 taggatcact aatcacctta aatagtttgc ttattgacag aatagatcct ttatcgattg
    18661 gagaagttgt ttttatatcc atagtgtttt tattcctttc atatgacctt ccgttcatat
    18721 ctatattacc atatgagcga aacaatgcta atcttttgtt tataaaagag aagtggcgtt
    18781 ctctttatct tgacaaaatg tatatagcga tataaaatga gtatacaaac atatgattaa
    18841 ttagtcatat gtaaggtgga ttcattacga tttacttttg gaataaacga aaggagtcag
    18901 aaatagcaga aatttatgtg attcaagaag taaatgatgg aaaacaaagg aattataaat
    18961 tatataggga ggcaatagaa gtggaaacta tccaagaaca acagtctcaa gaaaagcata
    19021 atcataatca caaccacgat cacgaccatg atcatgggaa aatgccagtc gttttgtact
    19081 ttgttggttt agcattagca gtgattgctc tatttttgaa tgaagattat cagttggtac
    19141 aaaatgtttt gttctcaatc gctacaatca gtgccgggta tcacgttatt cttcttgaag
    19201 gccttggaga aacaattgag aattcaaaag cacaaaaaaa gttcatacct aattctcata
    19261 ttttaatggg attagcggca attggagctt ctttgatggg gaatttttgg gaaggaacat
    19321 tattgattct tattttttct ggcgcacatt ttttggaaga ttatgcagaa ggaagaagca
    19381 aacgagaaat aacgaaatta ctcgaaatga atccaacgac tgcccggtta atcctgcctg
    19441 atggtagtac aaaaaatgtg gatgtcagtg aattaaaagt gggagatcaa cttcaagtgt
    19501 tgaatggcga tcaagtacct attgatggcg tcattttgtc tggatctaca tccattgatg
    19561 agtcgtctat tactggagaa agtatcccga aagagaaatc caaaggagat ggcgtcttcg
    19621 ggagtacaat caatggaaca ggtaccttca cgatgaaagt caccaaagaa aacaaagata
    19681 cagtattttc aaaaatttta caattagtga atcagaacca agataaccaa acaaaagctg
    19741 cgagtattat ccaaaaattt gaaccgaaat atgtcacagt tgttttaatt gccattcctc
    19801 tattcatgtt attggctcct tttctacttg attggacatg gtcacaaagt gtctatagag
    19861 gattggtcct tttagttgca gcttcaccgt gtgctttagc agcagctact gtctcggcaa
    19921 cgctatcgac gacttctaat ttagccaaaa aaggagtcct ttcaaaaggt agttcttacc
    19981 tgtctcaatt agccgatatt aaagcaattg catttgataa gacgggcacc ttgaccaaag
    20041 gaaaacctga agtaaccaat tattattttg ctgattccgt gaacgaagaa aacatgattg
    20101 atatcgtagt agctcttgaa aaagaatcca atcacccctt agcagatgct attctaagaa
    20161 agtttgagca aaaaaacaaa ctaactattg aagtagaaaa ccaaattggt aaaggattga
    20221 cgggagatta caaagggaga aattatcgta taggaaaacc gacttcgttt gatgatgttt
    20281 cagatgaata tagtcgttta aataatgaat gggcgtcaga aggtaagaca gtcgtatacg
    20341 tagcagtaga tgaagaggtt ataggcctta ttgcccttat ggacgttccg agtgaatatg
    20401 ctaaagaaac cattgaatat tttaggaaac aaggtataca caccacatta attactggtg
    20461 actcagaaat gacgggaaaa gcggttgcta aacaattagg aatagatgaa gtgattgcga
    20521 atgtcatgcc ggaagacaaa tccagaatta tagacgaaca aaaagaaaaa tacggcgtga
    20581 ccgccatggt tggagacggt gtaaatgatg ctcctgctct tgttaatgca gatgtgggaa
    20641 tcgctatggg agatgggact gatgtggcag tagaggtatc tgatttagtt ttaatgcaaa
    20701 acaatttatc taaattggta caatctcata atatttcttc gaaaatgaac cgtgttattt
    20761 ggcagaatat tattttttca atggccgttg ttgctttttt aattgtcgtt agtttcttag
    20821 gcttaacgga tattgcaatc agtgtcatta ttcatgaagg aagtactttg gttgttattt
    20881 tgaatggact tcgattgcta aaggcagtct aaagaatgat tgcacaactt caaattaatc
    20941 gagtaataga gataattaag gattacacta cattttttat ctctattact tgtttatatt
    21001 tatctagatg ttcaaagtca aaatgatata tcaaccatac tcttgtggcg cgggttgaat
    21061 ataagataca tgaaattgga taaaatcatg tatcaagaat cctatcctta tagggttctt
    21121 tttttttact ccccctaaac acacgatttc gtgttgtaat aactataatc atgtattcga
    21181 acagactaaa aaggagggat tgtcatgggc tataatgttc agccactacg gtcgcaacag
    21241 gagattaatg attttttatt ttgcctgcga cggaataaaa atgcggaacg tgacgttttc
    21301 ttgtttctaa ttggaattaa cagcggtctg cgcatgtccg acatcgttaa actcaagaaa
    21361 aaagacgtga ttacctcaaa aaacccgcgc atcgtggagc aaaagacagg gaaaacacgc
    21421 attttatact taagcagctt acaggacttg attcacggct ataccgcacc cttagatccg
    21481 gaggcttact tgtttcccag tactaagggc ggccatttag aagtcaatac ggtctaccag
    21541 atgtttcaaa aggtcgcgaa gctgttagga agggacgata tcggcaccca caccctacga
    21601 aaaacctttg gttaccacta ttacaagaaa accaaagacg tggccacctt aatggaaatc
    21661 tttggtcaca gcagcgagaa gattaccaaa cgctacatcg gaattaatga agatgaaatc
    21721 agtgaaacgt tattaaattt tcggttaggt ttttgattgt attatataat agataagcac
    21781 agtaagacgg ttataatcac ccacagtatt ggacaaatca accttttttt ggtatattat
    21841 atatacaaat gagagcaggc tcatagtcaa cttgatataa atccggaaca taagggtccg
    21901 gcgggtggcg agcaccaaaa ctaaactcga tacaaatccg gaacgtaagg gtccggcggg
    21961 tggcaagcac caaaactaaa cctgtacaaa aggctgctct tttgagcagc tttttttatt
    22021 gggaaaaagg agcattcttc gcacgcggca aaaaaagaat acaaacgcat gtctataaaa
    22081 gagacaaccc aaattaccag acagttaaac gcgatttata aggctgctca tctcttacaa
    22141 gaacattttg tagataaaaa agtatccttt gtcggagaag tctcaacggt agcgattatt
    22201 ttttcgacaa ctaattttat gcacctctgt ggcattgatt atcgcagagg cacacacttg
    22261 ttttttcaag atgccttaga caggaaaata aatttacagg atattcaaat caaaacggat
    22321 gggacgacct ttcagaaatt acaagtaata ggtagccttg atttattatt ggggaagcat
    22381 atttctattg ttggcagagg tgtttactct tctcttcgct atgatgcagc tattcgaaca
    22441 aggaaaaaaa tattggctct gtctttgaaa caaaacggat taatctatat ccctatctct
    22501 ttgttaaacc tatcatcaaa ggaaattgga cctggacaaa aagttactgg catttttagc
    22561 gaggatttaa catccgggga acttaaaatg attatggaag tcatcgatta atcattaata
    22621 gtgatatgcg acgtcatttt gaagtaatta ttctattttg ggttataaaa taaagcattt
    22681 taagacgatt tcaaatcatt catgtgggtt att
//
LOCUS       DAFCNS010000047        22345 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFCNS010000047 DAFCNS010000000
VERSION     DAFCNS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.047"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="LNE23_001757"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="LNE23_001757"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBK6931611.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2479)
                     /gene="purK"
                     /locus_tag="LNE23_001758"
     CDS             complement(1355..2479)
                     /gene="purK"
                     /locus_tag="LNE23_001758"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBK6931612.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="LNE23_001759"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="LNE23_001759"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBK6931613.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="LNE23_001760"
     CDS             complement(3376..4683)
                     /locus_tag="LNE23_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBK6931614.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="LNE23_001761"
     CDS             complement(4686..5267)
                     /locus_tag="LNE23_001761"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBK6931615.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="LNE23_001762"
     CDS             complement(5839..7131)
                     /locus_tag="LNE23_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6931616.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="LNE23_001763"
     CDS             complement(7128..7751)
                     /locus_tag="LNE23_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931617.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="LNE23_001764"
     CDS             complement(7754..8590)
                     /locus_tag="LNE23_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6931618.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="LNE23_001765"
     CDS             complement(8607..9494)
                     /locus_tag="LNE23_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6931619.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="LNE23_001766"
     CDS             complement(9510..10793)
                     /locus_tag="LNE23_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6931620.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="LNE23_001767"
     CDS             complement(11476..12159)
                     /locus_tag="LNE23_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK6931621.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="LNE23_001768"
     CDS             complement(12177..12794)
                     /locus_tag="LNE23_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK6931622.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="LNE23_001769"
     CDS             complement(12804..13628)
                     /locus_tag="LNE23_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBK6931623.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="LNE23_001770"
     CDS             complement(13621..14181)
                     /locus_tag="LNE23_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931624.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14355..15311
                     /locus_tag="LNE23_001771"
     CDS             14355..15311
                     /locus_tag="LNE23_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6931625.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMIHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15616..16572)
                     /locus_tag="LNE23_001772"
     CDS             complement(15616..16572)
                     /locus_tag="LNE23_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBK6931626.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCERLLTFIRESQEDAREHQENYWLRQPLYYETCFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILARIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16677..17279
                     /locus_tag="LNE23_001773"
     CDS             16677..17279
                     /locus_tag="LNE23_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6931627.1"
                     /translation="MQFGRFRLGMRTIKSAIAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17367..18866)
                     /locus_tag="LNE23_001774"
     CDS             complement(17367..18866)
                     /locus_tag="LNE23_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBK6931628.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMNEE"
     gene            complement(18882..19406)
                     /locus_tag="LNE23_001775"
     CDS             complement(18882..19406)
                     /locus_tag="LNE23_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBK6931629.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19581..21230
                     /locus_tag="LNE23_001776"
     CDS             19581..21230
                     /locus_tag="LNE23_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBK6931630.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILSMNR
                     SSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEGIR
                     YSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYNQEIIGAVCV
                     GLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMKKVLLGLEPQEIAEQLSE
                     KTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDFEVGKKISDNLYDVLFKE
                     TIEHQTTVRTQSMYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGTKQ
                     YINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVPAI
                     AGFLLGKINEAKEQSVEMLLETDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQKHE
                     RNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLIHS
                     IVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21199..21915
                     /locus_tag="LNE23_001777"
     CDS             21199..21915
                     /locus_tag="LNE23_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK6931631.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     LKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22017..22304)
                     /locus_tag="LNE23_001778"
                     /pseudo
     CDS             complement(22017..22304)
                     /locus_tag="LNE23_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
BASE COUNT         6579 a         4820 c         3910 g         7036 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    14281 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    14341 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    14401 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    14461 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    14521 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    14581 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    14641 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    14701 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    14761 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    14821 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gcactgttgg
    14881 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    14941 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatcagtc actatcttgc
    15001 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    15061 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    15121 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    15181 aagcaatgaa ttacgaaata aagaatatga tacacatgat tgaaggtgac ttgcccaatc
    15241 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    15301 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagt
    15361 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agatgcaagc
    15421 ttttgaacac cgtttaagga agaggtcgtg agcctgccgt tcagctccga gtcacaagga
    15481 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    15541 gacaggagaa caccgtttaa agaagaggtt gtgaactcag ctttttgacc tgaatattag
    15601 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    15661 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    15721 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgctaaaat
    15781 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    15841 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    15901 cacattggcc tgtgctcttc tcatagagaa gcaagtttcg tagtatagcg gctgtctgag
    15961 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    16021 acgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    16081 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    16141 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    16201 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    16261 aaccgaattt actacgatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    16321 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    16381 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    16441 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    16501 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    16561 tcctatacgc atttacttcc cccttttttt attttaatta tacttgaata atgaaaaact
    16621 agctatcaga caaaagttta ggtatgatag taaaaagacc aaaaaggaga atctctatgc
    16681 aatttggccg ttttcgacta ggtatgcgta cgatcaaaag tgccatagct gtttttttat
    16741 gtatcttgtt ttttcatttg actcaacgag gaatcccgat gattgctgca ttatctgctg
    16801 ttttttcttt gcgtcaggac ttgactacaa ccgtttcttt tggaaagtct aggatcatcg
    16861 gcaatacatt aggtggtaca ttagctatca tttatttttt tgtcaaaagc tattttcaca
    16921 atgatttttt ggtagaattg atcttgcttc ctattctagt tattattgtg atcgtcgtgt
    16981 cagatggtat taacaataat tccggtatca tttccgcaat cgctacgttg ttgctgattg
    17041 cgttgagcat ccctcaaggc gaatccgctt tgtatgctat ccaacgtgta ctagatactt
    17101 ttatagggac attcattgca atcggtatca atttctttct tcgtcctcct gaaacagaaa
    17161 agaaggaaga actatcagaa gatttggtcg aactccaaaa gaaagaacga ttgctaagaa
    17221 caaatttaca agagatagaa gctaagatcc aaaaacaaaa gaaaaatagc gaggactaat
    17281 tttcttttgc aataaaataa aaaaatggag agaagaactt taaaaagtct tcctccattt
    17341 ttgattgaac ggtgaagtgt actattttat tcttcattca tcgagttttg ttctgcctct
    17401 tttggcgctt catacatatt tacttggcta gtagtcccac gtgtcgacaa attctctacg
    17461 atttccttca agtcaagtcc tgttgtcgct tttagtgttt cttgagtaga agaaagcaaa
    17521 tttgtggcat aatttgtcac tcggttcgct ccactggttt ctcctgaact gcttcctgta
    17581 tcgacaacag agattttttc gatatttcct agcggctgtg ctgcttcgcg catcaattgc
    17641 ggtagcatat cgataaccat actcaagaca gctgcttcgc catattcctt gaaggcattg
    17701 gcgatctttt gttttgcttc ggcttcggct tttccttttg ctagtgctgc ttcggcttct
    17761 gcttgtccag ctaaacgtgt cttattggct tccgcttctg ccagtgcttc cacacggaat
    17821 cgttctgctt cggcttcagt cacttctttg actttttgag cttgtgcttc ttgttcttta
    17881 gcgtaccgat cggcatctgc ttttttcttc acttccgaat catattgctt ttcacgacgt
    17941 gtgatttctt tttcttccag ttcgatttgt ttttgacgtt ctacgatttt aatctgcatt
    18001 tcctgttcga ttacttgttg ttgtgctcgg gcactttcta aattgtaggc ttggtctgct
    18061 tttgctttag caacatcttg ttcttgtttg taggttgcta atttcagttc tttttctttt
    18121 aatgattctg cgatctctgt ttgtcgctgt aattcagctg cttgtgattc tttttctgct
    18181 tgagcttttt tgatacgcgt ttcttttaga gcttccgctt ctgctatatc tgcatctctt
    18241 ttgacttggg cgattcttgg tttccctaaa gagtcaagat aaccgttttt gtctctgacc
    18301 tctttgatag tgaaagaaac gatgatcaac cccatttttg caaggtctac agaagcgact
    18361 tcttgtacgc tttgactgaa cttatcgcga ttttgataga tttcttctac agtcatagaa
    18421 ccaagaatcg agcgcaagtg tccttctaat acttctcttg cttcattttc cagttcttct
    18481 cttgtcttgc ctaaaaactg ttctgctgct gtagcaatct cttctacaga tgaaccgatt
    18541 ttgatgatcg aagtaccatc acacatcacc ggtacgcctt gttccgtata cacttcaggg
    18601 gtggagacgt ccaatttact cgataataga ctgatccgat tcgaacgttg aaaaactggc
    18661 aagacgaatg cgccacctcc acgcacaatt ttgattttgt tattgctttc atcaatgtgc
    18721 acatttttgc ttcctaaata acttccactg atgatcaacg cttcatccgg ttttgctgtc
    18781 tggtacttcg ctacaaagac gatcagcaac atcagtaaaa tcaaagctgc gattgcgatc
    18841 gcgataaaaa caactggtat ttccatttaa cacacgctcc tttaagcttt tatcatgctt
    18901 tcataaggca tgacataaca gattctttcc ttgatctcaa tgatcaatac tttcgttccc
    18961 cgctcagctg tctgttcttg ggggctgtaa aaacaagctg gacgactaat ggaaccggta
    19021 acacttttca actgaatttc tcccatacct cgtacaggga tcggtgtgat cacaacagct
    19081 acttgtcctt ccagagtttt ttcagaagac gacatcgttg attcggcatg tttcatcggc
    19141 atcaatatat agaaattaag caaaaatacc aacactatcg atatgcctcc actagcaata
    19201 aataaaaata aagatgattg atctgttaaa cgctcaccta gaaaaccaaa caagcttgtg
    19261 aaagttaacc aagggataat aagcatcgga tcaagcgggc catcaaaatc gaatacatct
    19321 ccaaaaataa tcaataggac tgccaatcct gcacaaatca gcaatgtata ccaataaatc
    19381 gtctcaagtg cgattccccc tatcatatac acgcctcctt ccatttctac gtctatcata
    19441 ccaatgtttg aaaattctgt atagcttctt ttaccgactc agtggtgttt tttataggaa
    19501 ggttgtgttt atcgttgaca atttgttaaa tttttgtttt catgttaaaa ttttcacaat
    19561 aaattctttc gaggtagcct atgtttcctt tttttaagaa aaaaccatta aagctccaga
    19621 acacgatcat ccttgtcgtt tgtttctcca cattcgtttc tttgattgtt tctgccatat
    19681 tgatcaggaa ttttattctt tcgatgaacc gatctagtgt gtcagataaa atctctagca
    19741 ttgctaaaat cgttgccaat gataaactca tccaaaaaaa gctgcagact ggtgacccaa
    19801 cacatgcgat tcaaggaata acaaatgaaa tcatgattgc atcgaattta gattttatcg
    19861 tggtcatgga ccctgaaggc attcgatatt ctcacgcaga cccaagttac atcggtcttc
    19921 ctttctcaag tcatgaagat gtgaaaaaag tgattggagg acaagaattt ttctctgaaa
    19981 aagaaggaat gttagggttc ggcttccgct atttcgttcc tatttattac aatcaagaga
    20041 ttattggtgc agtttgtgtt ggcttaacga acaacacgat tgacaatcaa gtcaaagatg
    20101 gacaaaaaat gattgttttt tcattgatta tcggacttag cgtaggcatc ataggcgccg
    20161 tttttcttgc ccaaaaaatg aaaaaagtac tgcttggact tgaaccacaa gaaatagcag
    20221 aacagttaag tgaaaaaacg attatcgaaa atgaagtaga tgaaggaatc ttagccatct
    20281 cttctgataa aactatcctc ttgatcaata cacctgcaaa aaacattttc taccaaacag
    20341 agcagctatt ggatttcgaa gtaggaaaaa aaatcagtga taatctatat gacgttttgt
    20401 ttaaagagac gattgagcat caaacgaccg ttcggactca atccatgtat ttaaatggga
    20461 tagaaactat ttctagcgtt acacctcttt ttagcaatga ccgtttttta ggagcagtcg
    20521 caactttacg aaatcgatca gaagtaatga aattaatgga ggagcttggg gggacgaaac
    20581 aatatataaa ctccatgaga gctcaaaccc atgaattcat gaataaactt catgtcattt
    20641 ctggtttgat tgatttgaag aagtatgaag aagtcaccca ttttatccag cagctaaatc
    20701 aaaattataa aaaagagatt ggccaagtct caagtctgat caaagttcct gcaattgccg
    20761 gttttttatt aggcaaaata aacgaagcca aagaacaatc tgttgaaatg ctgttagaaa
    20821 ccgattcttt tatccctaaa atggagatgg atgactccat ccattcactt ttgcaagtgt
    20881 tagggaactt acttgataat gcaaaagaag ccgtgctaca aaaacatgaa agaaacggac
    20941 aggtccggat tcatttaaac tatgacacag aagctgaagt attcatcgtg accgtctgcg
    21001 atgacggtgt cgggatatca gaagaaatct cagaaaaaat atttacgcta ggtttttcga
    21061 cgaagggaat taatcgtggg tttgggttga atctcatcca ttcaatcgtc aaacgccacg
    21121 gtggattttt agaattttcc aaacaaacaa atggcggtac aatcgtctat ctagaattac
    21181 caaaaaagga agatcaaaat gatgacaaat gtcctgataa tcgaagatga tccaatggtt
    21241 gcagaattga atcgtacttt tgtggaaagc atccctaata tgaacgtaat cgaaagtttc
    21301 agaaatgtag aagaaggaaa aaaatatttc agacaaaaaa acgcagatct gctgctgctt
    21361 gatgtataca tgcctggcga aagtggtatc cattttttat cttggcttag acagcaagga
    21421 aatgatatcc cagtgatttt gatcactgct gcagatgata tcgaaaccat aaagaatgtg
    21481 atccgctacg gtgccttaga ttatttgatc aaaccgttca ctttgcaacg ttttcagctg
    21541 gcgatcgaaa agtatcttgc tttctctcag acaatccaaa cgactgaaaa agccgatcag
    21601 aaattgatcg atcaagtatt caaccacacc actcccattc acgaaatacc atcattcatc
    21661 gatttgccta aaggtctgtt aaaactgact ttaaaaaaaa taagtgcatt tattctaaaa
    21721 gaagatacta ttttttcaac aaaagaactg gctcaaaaaa gctcactgtc acgtatatca
    21781 gtcaagaaat atttgaattt tttggtagaa caagatatat tgaacgaaag aatcaaatat
    21841 ttggaaatcg gccggccgct aacctgttat gaattgacaa agaatgggcg gaaaaagctc
    21901 acagattact gttgagtcat cgaattatcc catacaaaat agtccaagta tacagaagta
    21961 acaactgttt acttggacta tttttaaaca ttttattgac gaatatccat gttttcttat
    22021 cttaattgac gggctttagg aagagatacc tcatttttcg tttttccagt ttctattatt
    22081 tcacgaaagt tctcgaaact tgtcgaaatc atatttgata atgcctcatc tgtatttgaa
    22141 cctacatgcg gggttacaag aactctaggg tacatttcta ctaattgctg gatagcaggg
    22201 tctggaatcg tctcccatgg cttgaaggat cggaaaaaaa cctctgtttc atttgcaaaa
    22261 acatcggttg caaaaccagc taaatgattg cttttcaaag ctcgtgaact actcaccact
    22321 tagctaaacc taacggtttc ttacg
//
LOCUS       DAFCNS010000048        22073 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFCNS010000048 DAFCNS010000000
VERSION     DAFCNS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.048"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..221)
                     /locus_tag="LNE23_001779"
     CDS             complement(<1..221)
                     /locus_tag="LNE23_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931632.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVT"
     gene            complement(470..670)
                     /locus_tag="LNE23_001780"
     CDS             complement(470..670)
                     /locus_tag="LNE23_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6931633.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(975..2207)
                     /locus_tag="LNE23_001781"
     CDS             complement(975..2207)
                     /locus_tag="LNE23_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK6931634.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2461..3030
                     /locus_tag="LNE23_001782"
     CDS             2461..3030
                     /locus_tag="LNE23_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6931635.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3208..3648)
                     /locus_tag="LNE23_001783"
     CDS             complement(3208..3648)
                     /locus_tag="LNE23_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBK6931636.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3806..4570
                     /gene="map"
                     /locus_tag="LNE23_001784"
     CDS             3806..4570
                     /gene="map"
                     /locus_tag="LNE23_001784"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBK6931637.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4602..5525
                     /locus_tag="LNE23_001785"
     CDS             4602..5525
                     /locus_tag="LNE23_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBK6931638.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5601..6740
                     /locus_tag="LNE23_001786"
     CDS             5601..6740
                     /locus_tag="LNE23_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBK6931639.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6733..7533
                     /locus_tag="LNE23_001787"
     CDS             6733..7533
                     /locus_tag="LNE23_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6931640.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7533..8360
                     /locus_tag="LNE23_001788"
     CDS             7533..8360
                     /locus_tag="LNE23_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6931641.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8338..9072
                     /locus_tag="LNE23_001789"
     CDS             8338..9072
                     /locus_tag="LNE23_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6931642.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9172..10038
                     /gene="rfbA"
                     /locus_tag="LNE23_001790"
     CDS             9172..10038
                     /gene="rfbA"
                     /locus_tag="LNE23_001790"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBK6931643.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10052..10624
                     /gene="rfbC"
                     /locus_tag="LNE23_001791"
     CDS             10052..10624
                     /gene="rfbC"
                     /locus_tag="LNE23_001791"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBK6931644.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10646..11674
                     /gene="rfbB"
                     /locus_tag="LNE23_001792"
     CDS             10646..11674
                     /gene="rfbB"
                     /locus_tag="LNE23_001792"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBK6931645.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11772..12623
                     /gene="rfbD"
                     /locus_tag="LNE23_001793"
     CDS             11772..12623
                     /gene="rfbD"
                     /locus_tag="LNE23_001793"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBK6931646.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12658..14691
                     /locus_tag="LNE23_001794"
     CDS             12658..14691
                     /locus_tag="LNE23_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931647.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14735..16015
                     /locus_tag="LNE23_001795"
     CDS             14735..16015
                     /locus_tag="LNE23_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBK6931648.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16225..17031
                     /locus_tag="LNE23_001796"
     CDS             16225..17031
                     /locus_tag="LNE23_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931649.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17043..18263
                     /locus_tag="LNE23_001797"
     CDS             17043..18263
                     /locus_tag="LNE23_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931650.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18253..19839
                     /locus_tag="LNE23_001798"
     CDS             18253..19839
                     /locus_tag="LNE23_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6931651.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19878..22016
                     /locus_tag="LNE23_001799"
     CDS             19878..22016
                     /locus_tag="LNE23_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6931652.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6972 a         3737 c         4571 g         6793 t
ORIGIN      
        1 tcagtcacga tatcaaacaa aaagtataca cccttcttca ttctagtttt cgcagcagga
       61 attttgaaat agttcttatt agaatagtag agattgatta ataagctatc ttcgattgct
      121 aaaaagaaaa cttctgaatt ccatacttta taaaaatctt tgataaatct attcgaaggg
      181 tcaaatttaa accataattg tgtttttcct tccatcagca taaaagttca cctcaaaaag
      241 agtatacgaa cgaatgttct ttttgtgaag gcggaattta atatactgaa taatagaaaa
      301 caagtacttg tgccaaatta tgtgccaaaa aaattcgaat tcaacaaaat gtaaacaaat
      361 agaaaacctt ctatattaag atttcctgca ataacaaaca caacagaaga cgtgtaatag
      421 ttagtcagga acgtacaaat aacccctgta tcctttgcgg tacagggggt tattttgtat
      481 aagtagacat tgtagtttct aaactgctat gtcctaaacg tttcgatacg ctaagtatat
      541 ttactccttg ataaagtaaa atagatgcat gcgtatgtct tagtccatca ataaacagtc
      601 gaagagaaaa tagggaaaaa ttgattacta aagaaaaact tactaggtcg acggtgtagt
      661 tgattttcat acttcaatag attcgaatta aacgatttcg attggcttat accagtccta
      721 tccaacgaaa gatatatcgt tcttaactct agcggtataa cttgaagctt tacgtaaagc
      781 caatgggaat gaactggaga tgacttttta ttatttttag taatagctga tgggttaata
      841 cttacaactt tccttttcaa agtaccatat aaaaaaacgg tggggttgtt tagcgaaata
      901 aaggatctaa gccaatagct tagatccttt attctatgct ttatattatt ttttgcatgt
      961 gtgcgaaagt atactcaagc ccaatcgcca tttcggaaaa tcggtactgt agagccatct
     1021 tcacggattc cgtcgatatc catcttgtca gatcctacca tgaaatctac gtgggtttga
     1081 cttctattca atccagcttc tgctaattct tcatcggaca tttctgtacc gcctttcaca
     1141 ctgaaggcat aagcagaacc tagtgctaag tgattcgatg cgttttcatc aaataatgta
     1201 ttgaaaaaga tgatgccaga ttgagaaata ggtgaagggt caggtacaag tgcgacttct
     1261 cctaaacgac gcgcaccttc atctgtatca agcagttttg ctaagacatc ttctccttgt
     1321 tcggcagaaa aatcaacaac ttttccatct ttaaaagtaa atttcatacc cgagatgatg
     1381 gtgccagcat aactcagtgg ctttgtactt gaaatgtaac catctactcg atgactgtca
     1441 ggtgctgtga acacttcttc cgtcggcata tttgccatga atttttctcc acgagcatta
     1501 tagcttccag cgccttccca aagatggttt ttaggcaatc cgataatgat atctgtaccg
     1561 ggagcagtat agtgaagggc agaaaattgt tctcgattca attcttctgc tttttttgct
     1621 aatttttcat catgcttctt ccatgctaag acagggtctt cttcatagac acgagtcgtt
     1681 ttgaaaatct gatcccataa tgcctcaact tgtttttctt ctggcagttc tgggaagact
     1741 tttgctgccc attgtttacc agccgcagcc acgacagtcc aactgacttt atttgcttga
     1801 gttgccttgc gtaaattcat tagtgctttg ccattcgctg attgataaga cgctactcga
     1861 tggctatcta caccagcaaa tgcatcggga tcggcagaaa caacgctgat cctgcttgct
     1921 cctttttcaa gccattcatc cgcttgatcg attttagatt gtggtacatt ctcaatacga
     1981 tccgttgcag catgtaaaag aaattctctt tggatctggt cgtcagtcca ttgaacgata
     2041 acctcagcgg ctcctagttt ataagcttct tgtgtgatca atcgagcaag gggtgcctgt
     2101 tccacgctga tctgtaaaac aactgtgtgt cctttttctg tggctacgcc agtttctgca
     2161 attaagcgtg catatttttt tagaagttca ttaaaatcag ataacataat aaaaattcct
     2221 cctcaccatt ttttctggta actattagca tgataccatt taattatgat tcaaacaatg
     2281 aggctttaaa taaaaaaagg aggttatttt tttaaggtta agtttcatga tatatgttac
     2341 tacttgctat ttcagcttgt gaagaaaatc aaaaactaat tttgcatatc aaaaaaacat
     2401 gttacaatga aattaatcaa tggtggaagc gttataaaaa aattaaggag attaaaaaat
     2461 atggcaagaa aaaagacgat tactagagaa cagattttgg cagctgctta tgaagtagct
     2521 gccaccgatg gatttacacg atttactgcg agaaacatcg cgaacaaaat gaattgttcc
     2581 acgcaaccga tttatcttga gtttaaaaat atggatgact tgagacaagc tctttttgag
     2641 cagatctatg atcatttaca atatgaagtg ttcactgttg agcatacagg gaatacgatt
     2701 atcgatcttg cttttaatta tatccatttt gcaagaagag agaagaagtt gtatcgttca
     2761 ctgtacttag aagaatatgg tggaggaaaa gagatgcaga atttctctta tacttacttt
     2821 gtgaatacag tgaaacgcga tccagcttat gtgaatcttt cagaagagca gatcgaatct
     2881 ttgtataatg ggacatggat catagctact gggatcgcag ctttgatgtc ttcaagcatc
     2941 atccatccaa ctgatgaaca gattgaaaag atgatccaag acagtataga tgctatcttg
     3001 aaattcaaag aaccaatcga aatcgattga atatactcaa aagttccaca aatcagtagg
     3061 taacaagaga tgtgtcaaaa taaggaaaag ttcgtttttc ttacagcgta tgcattttta
     3121 actcccgaag aactcttcca tgaatgcaat gaataaaaga gccatacaga ttgaataaat
     3181 ctggatggct cttttattgc aagtatatta aatttttgcg actagttctt tagcgaaagc
     3241 ttctaaattt tcgatatctt cttcttcagc attcagatcc actttaacat tttcggctcc
     3301 ttttgatgcg cctgtttttg caaaagcagc atcgaaatca tccaccgatt tgcagaattc
     3361 atcgtaaaaa gtatcaccag atccgcagac accgtaaatt tttcccaata gatccaattc
     3421 ttgcaagtct tcataaaagt ctacgatctc atcaggcaga tcgccatcat cgtatgtata
     3481 agtagctact acgcaaatat ctgcctcctc gaattcatct gcatctactt gggtacattc
     3541 gttgatttcg acttcgatat ccagattttc aaatgcttct gcaacaatat cggcgatttc
     3601 ttcagtgttt cctgtcatgc tagcatatac gatttttgct aaagccatga tgtgcctcct
     3661 cgtttataat tatttaacaa ttatgaatta taccaaaaca tcattgaaaa gaaaataaaa
     3721 cctaaagaca cctggggaat gtcatttggc taaatgtatg ccgctatgtt aaaatgagag
     3781 ggatttgtaa aaaggagacg aaaatatgat tactttaaaa tcagcacgtg aaattgaaat
     3841 gatggcagaa tctggagctt tattagcaga tgtacataaa aatctgcgtg attttataaa
     3901 accaggaatc acaagttggg atattgaggt atttgtcaga aactatatcg agagtcatgg
     3961 cggaattgct gcgcaaattg gctttgaagg gtatgaatat gcgacttgta tcagtataaa
     4021 tgatgaaatt tgtcatggat tccctcgtaa aaagcctttg aaaaacggtg atttggtaaa
     4081 agtcgatatg tgtatcgatt tgaaaggcgg catttctgat tcttgttggg cctatgtagt
     4141 tggagaatct acacctgaga tcgaccgatt gatggaagta acgaagaaag ctttgtacat
     4201 cggtattgag caagcacaag tagggaatcg gattggtgac atcggtcatg ctatccaaac
     4261 ttatgtagaa ggcgaaaatc tagcgatcgt tcgtgatttt attggtcatg gcgttggtcc
     4321 tacgattcat gaagaaccag cagttcctca ttacggagaa gctggaaaag ggttgcggtt
     4381 gaaagaaggc atggttatca cgatcgaacc tatggttaat accggaactt ggaaaatgaa
     4441 aatggatcct aacggctgga ctgcgtatac aagagacggc ggactttctt gccaatatga
     4501 acatacattg gctattacta aagatggtcc aagaattttg acatcacaag gagaagaagg
     4561 cacttattga tctatccagt caataggtac gggagaggta catgaaagtt tggaacaaaa
     4621 taaaacaaaa tgaaaatctg atgcgcttta ttgagacgac tcaaaagcgc atcactgatt
     4681 cagaaatgag tacgacctct gtcgtcgttg cttattattt actcttgtct ttatttccat
     4741 tgattattgc ttttggtaat attttaccgt ttctccacat cgatcaagag actgtactga
     4801 cctatttacg agaagtgatt cctgagaccg tttaccagtt tatcggtcct gcaatcaaag
     4861 accttttgac acaaagttca ggaggcttgt tatccgtttc tgcattagcg gctttatggt
     4921 ctgcaagtca aagtatcaat gctttgcaaa ttgcgatgaa taaagcgtat ggcgtagaaa
     4981 atagaaaaaa ttttttgatc gtccgtttct tctcactaat tgtcattgtc ttgttcatga
     5041 ttgctattag tggggtaact atcgttcttg ggttgggaca gctgattttg gatgctattc
     5101 agccaatcgt acaaataccc gtgaacttta tcgatcagtt ccaaactttg aagtggccaa
     5161 tcacattggt agctttattt gtcatcatgt tcttgattta tttaattgtc cctaatgctc
     5221 agttgaagtt gaagacgatc attccgggca cgatcttcgc tactgtcggc tggatgctgt
     5281 tatcacaagt gtttagtatc tatgctaggt attttgcaac gaggatcagc ggttatcaga
     5341 tcataggaag ttttattgta ctcatgcttt ggcttaattt tgctgctacg atcatcattt
     5401 tgggcggaat catcaatgca gttgttcaag aatacgtgac gggtcatcaa atcaaaaaac
     5461 gtcgagatcc aagccgaaaa tggttcaata aattgaaaac taaattttct cataaaaaaa
     5521 agtagaaaca agagttctta ttgtaaattt atatcagtaa tttataataa ttatgactct
     5581 taattaaagt aggtgcccat atgcttgttt cttttagtat ggttataaga ttttttctga
     5641 caattttatt atcactgata ttaacacctg tattcaaaat catttcagta caaacaggta
     5701 tggtcgacaa accaaatgaa agaagaataa ataaagttcc aatgccttca gcgggcggac
     5761 tgcctatctt tatttcattt gttatatcga ctttattctt attccgtaaa attatccctc
     5821 aatcgtatat tgtaccgatc ttgattgctt ctttagtaat catagtcaca ggagtgctcg
     5881 atgataaata tgagctgaca cctaaacaaa aaagtatagg gatcttgatt gcttctctta
     5941 ttatttattt tgtagcagat atacgtattg attccttcac gcttcctttt attggatata
     6001 tccaactagg ctggcttagt tttcccgtaa cgattttttg gatcttcggt atcacgaatg
     6061 cggtcaatct gattgacggt ttggatgggc tggcggcagg gatttcattg atcggtttaa
     6121 ttacgatagg aatcattgga tattttttcc tccatgcttc tacagtgtat attccagttg
     6181 ttattttctg tcttgtagca agtattatcg gtttttttcc ttacaatttt tatccggcaa
     6241 agatttattt aggcgataca ggagctctat tcctagggtt tatgatggcc gtcctttcac
     6301 tacaagggct gaagaacgtg acttttgttt catcgatttc tttgctgatc gtcatgggag
     6361 tgccggtaac agacacgttt tttgctatca ttaggagaaa agcaaaccgt gtttcttttt
     6421 ctacagcaga taaaaaacat cttcatcacc gactaatcag tttaggcttt actcataaag
     6481 gtgctgtcct gacgatttat gcgatggctt tgatgttttc tttcacagct atggtcatga
     6541 attatactgg aagaatcgga acgatcatat tgattattgc catgttgttt gctgcaattt
     6601 tattatttga attgattggt ctgatcaatg agaaacatca gtttatttta aaaacattgc
     6661 gatttttagg gaataaagag taccgtacgc aagtaaaaca gaaatatttg aagaaatgga
     6721 accgtcatat agatgaataa caaattaaac cagccgatcc ttattagtat tgtgacggca
     6781 aacagtgata aaatttttcg gacactagat aattttattt caactgtaaa agatgaaaca
     6841 gcagtaaaaa tccgaatttt tgacaacaat tcacttcccg agtacaaaaa aagactaaca
     6901 gagtatagga aatatcccta catcgatatc tattttcatg atgaaaattc cggatttggt
     6961 tatggacata atcacaatct gatagctagc gattctcgct atggcattat ttgtaaccca
     7021 gatattttag tggatgaaga gacagttaca gcattggtct tgttattgaa agaacatccg
     7081 gaatgtgcga tgttagcacc taagattctt aatgaagatg gaacaactca gcacctcatc
     7141 cgtgagcagc tggctgtttt tgattacgtt ttgcgctttg tgcctttcaa atgggtcaaa
     7201 aaaaagtttg acaaacgact ggctgtgttt gaatgccgag gattgcctac tgatgctaat
     7261 agttatgtac ggatgatctc aggtagtttt atggtagctg atattgataa atttaaagag
     7321 ataaaggggt ttgatgagcg tttcttcatg tactttgaag acaacgactt gtgtttgagt
     7381 tttgaaaaag caggttacaa gttattgtac acaccctttt atgcagttat ccatttttac
     7441 gggaagggtg cgcatcgaaa tgcaaaactg tttcgcgtgt ttttgacttc tatgatgaaa
     7501 tttttcaata agtgggggtg gcgttttttc tgatgaaacc agaaatatta gtagtcgttg
     7561 tgctttatca gcaactattc agtcaatctc cagcttatgg accattaagc aaagcactta
     7621 gcgaaaagca aattcagctt attatttacg ataatagtcc tcaaaggcag caaaatgagc
     7681 tgtttgaaaa agaaaatatc gtttatcatc atgatccgca aaatcctggt ttagcggtgg
     7741 cttataattt tgcgttgtct caagcagatg aaggaacacg ttgtctcgtc actctggatc
     7801 aggatacgag gctagtcgac gattattttg aactattgcg taaagtaacc tttacagatg
     7861 aatgtgttgc tgcagttccg atgatttttt ctgggagccg acaagtttct cccctatatt
     7921 cagatcgtta tatcaatcgt acagctcaag cagtagaagt tgaaggccca acagctgaac
     7981 gaatcatggc aatcaattca ggtgttgcct ggtctgtcaa atttctaaaa aagattggcg
     8041 ggtttaatcc tgctttttct ttagattttt tggatcattg gctgttttgg aaagtggatc
     8101 agttggaaaa aactgtggag atccttcctg tacgaatgga acatgatctt tcggttttag
     8161 actatgaaaa agtttcaagt aatcgatacc gttctatttt acaagcagaa aatcgatttt
     8221 atcatgaata tgatcaaagg catttatcca gtcaccgacg acaattattg ttaagaacag
     8281 ctaaacagtt cttgaccgtc aaaaaccggc atatctggcg gatgactttg cgctcatatg
     8341 ttaagaattg gaaggtgtga attcgtgcgt tcggtttgta tagctacctt taatggaggc
     8401 agatatttaa gagagcagtt agatagtatt ttatctcagc tttcagcaga agatgaagta
     8461 gtcatttctg atgatgggtc agtagatgat acatgggata tattaactga atacgcagaa
     8521 aaagataagc gaatccagct tttttcgggg cctcgtcaag gattgatcgc taattttggt
     8581 tatgcgatcc agcagacgaa aggagatcta atctttttgg cagaccaaga tgatgtttgg
     8641 ttggagacaa aagtgaaaaa gatcactgac tattttgagc agaatccgga tcagctagta
     8701 gtagtttctg atttaatcat tgtggacagc gagctgcaag agatccatga ttcgtatttt
     8761 tcgtttcgaa attccaaaga aggatggctt aataatgtga taagaagtca ctatattggt
     8821 gctggtatgg ctttccggtc ttctttaaaa cccgatattt tgccgatccc gtcagaagtg
     8881 ccgatgcatg atatgtggat tggtttgctg ggaggaaaga agactgggtt cctaagagaa
     8941 ccgttaactc tgtaccgaag acatgattta aacgcgagtg agattgaaac acattctaaa
     9001 ttacggcaaa aaatttcttg gcgtgttcat ctgattacgg cgttgatcaa acgaaaatgg
     9061 tttaaacgtt gataaaagat gtttaaatcc ttaatatttc catgacaaat ataacaattg
     9121 atgagataat aacaaagtga aatttgaaat caaaaaggag agcagatatc catgaaggga
     9181 atcattcttg ctgggggcag cggaacacgt ctgtaccctt taacaaaggc aacatcgaaa
     9241 caattgatgc cgatttatga caaaccaatg atttattatc caatgtctac tctgatgttg
     9301 gcgggaatca aagaaattct gattatttca acgccacaag atacaccacg tttcaaggaa
     9361 ttatttggaa acggaaatga tttaggactg cagatcgaat atgcagtaca agaaagtcca
     9421 gatggattgg cacaagcgtt tatcattgga gaagaattta ttggagatga tagtgtctgc
     9481 ttggtattag gggacaatat ttactatggt ggcggtctgt ctaaaatgtt gcaacgagca
     9541 gcttcaaaag atacaggcgc gacagtattt ggctatcatg tcaatgatcc agaacgcttt
     9601 ggtgtagtag aatttgatga gaatatgcaa gctttgtcga ttgaagaaaa gcctgctcag
     9661 ccaaaatcaa attacgctgt aactggcctt tatttttatg acaatgaggt aatttctatc
     9721 gctaaaggta tcaaaccttc agaacgtgga gaactagaga ttacggatgt caataaagca
     9781 tatttggata aaggaaaact atccgtagaa cttatgggac gcggatttgc ttggcttgat
     9841 acaggaacac atgaatcatt gcttgaagca tctaccttta tcgaaacaat tgaaaaacgt
     9901 cagaacttaa aagtggcttg tttggaagaa attgcttacc gtatgggcta catcgataag
     9961 gaacaattgc tggcccttgc acagccattg aaaaaaaatc aatacggtca atatttattg
    10021 aatttggccg ctgaataagg agtccttaca gatgaaagta acagatacaa aattaacaga
    10081 tgtcaaaatc gtagaaatgg acgtctttgg cgatcatcgc ggtttcttca cagaaagtta
    10141 ttcaaaagca aaatttgcag aacatggatt gaaatatgac tttgttcaag acaaccattc
    10201 actatcagct gaagctggtg tccttcgggg gcttcatttc caaaaaggag aagctgcaca
    10261 gacaaaattg attcgggtag tcactggagc agttttagat gtagtagtgg atatccgcaa
    10321 aggtagcccg acttatggtc aatgggacgg atttatctta tctgaacata atcatcgaca
    10381 gttgcttgta cctaaaggat atgcccatgg attcgttact ttgacaccaa atgtgaactt
    10441 cctttataag tgtgataatt actataatgc agaagcagat gggggcatcg catttgatga
    10501 tccagattta gcgattgatt ggccaattga tccatcaaga gcgattatgt cggaaaaaga
    10561 tcaccgtcat ccgacattaa aagaatttga agcagaaaat ccatttgttt acggagaaat
    10621 ctaagaatta aggagaaatt ttcttatgaa aaacatcatt gttaccggag gtgccggatt
    10681 catcggctct aatttcgttc attacgtggt aaacaatcat ccagaagtgc atgtcactgt
    10741 tttggataaa ctgacttatg caggaaacaa agagaacttg gccggacttc ctagcgatcg
    10801 cgtagaattg gtcgtaggag atgttgcaga tgcagaatta gtggaccgtc tagtaaaaga
    10861 agcggacgca gttgttcatt acgcggcaga atcccataac gataattcat taaaagatcc
    10921 tttcccattt gtacaaacga atatcattgg tacgtataca cttttagaag cttgccgcaa
    10981 gtatgatgta cgctatcatc acgtatctac tgacgaggtt tacggcgatc ttccattacg
    11041 tgaagacctg ccaggacatg gggaaggcga aggagaaaaa ttcactgcgg aaacaccata
    11101 taatccttca agtccatatt cttctacaaa agctggttca gatctgttag taaaagcatg
    11161 ggtgcgttct tttggcttac gtgcaaccat ttcaaactgt tcaaacaact atggtcctta
    11221 tcagcatatt gaaaaattta ttcctcgcca agtaacgagt atcttaagcg gtattcgtcc
    11281 aaaattatat ggcgaaggaa agaatgttcg tgactggatc catacgaatg atcattcttc
    11341 agcagtgtgg ctgattttga cgaaaggcag aattggcgaa acctatctga tcggtgcaga
    11401 cggtgaagaa gataataaga ccgtgatgga attgatctta gaaatgatgg gccaaccaaa
    11461 agacgcatac gaccatgtaa acgaccgcgc agggcacgat ttgcgttatg caatcgattc
    11521 gacaaaattg cgagaagagc taggttggca gcctgagttt acgaacttcc gtgatgggtt
    11581 ggctgacacg atcaaatggt acgaagaaca tgaagactgg tggaaaaaag aaaaagaggc
    11641 cgttgaagca gcatatgcca aaaatggaca ataatacaga ggaaatttca ctgtattaat
    11701 ggaaaaacac atgtgaggca ggcgctcgcc tgtctctttt ttaaaataaa aatcaaggag
    11761 cggataacca tatgttttta ttaactggag gaaatggaca attaggtaca gaattgcgcc
    11821 atttattaga tgaaaaaaat ctgccatatg tttcaacaga tgcaaaagac ttggatatca
    11881 cagatgctga gaaaacaatg gcttatatta cagaattaaa acccgatgtt attttccatt
    11941 gcgcagctta cacagcagta gataaagcag aagaagaagc aaaagaatta gatgagaaaa
    12001 tcaatgtcga tggcacaaga aatgtagcac ttgcagcaaa agctgtgaat gcacaattcg
    12061 tctatatcag tacagactat gttttcgatg gaaaaaacaa aaatgtagaa tacgaagtag
    12121 acgaccaaac gaatccacta aatgaatatg gtcgtacgaa attattaggc gaaaaagcag
    12181 tagaagagat tttagaagac tattatatca tacgaacttc ttgggttttc gggatctatg
    12241 gacacaattt cgtcttcacg atgcaacgcc tagcggaaac ccgcgacaaa ctgactgtag
    12301 taaatgacca gtttgggcga ccaacttgga cacgtacttt ggcagaattt atggtatatg
    12361 tcatcgaaga aaaagcacca tttggtattt atcatttgtc gaacgaaaat agctgttctt
    12421 ggtatgagtt cgcaaaagaa atattgaaag atacagatgt agaagttgca cctgttacgt
    12481 cagaagaatt tccgcaaaaa gcaacacgtc cgcagtattc agtcatgagc ctgaaaaaga
    12541 cagaagcgtt aggttttgtc ataccaactt ggcaagaagc acttgcgcaa atgctggaag
    12601 ctgccaaaaa aatgaataag taatttcctt tttatcaaat agaatttgga gcatgatatg
    12661 gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa aagcgaatca ggcagattcg
    12721 aatttggtgc ttgacctgtt tctaaaactc cgctatctta ttggcgtttt agtcatcgtg
    12781 gtcatcgtta cttttaattt aaacggaagt tcacttggtg tttgggataa atatgtgtcg
    12841 caaagagacg atgggaaaaa aactgatgtg atttttggac agaatcggga agtgcggtca
    12901 gacgaatggc tggtgcagac acctttttac ctctcacaag ctgaagagga cttttcgttg
    12961 gtgaacgagg attattcatt aaacggacaa aacatgatca ttgcgtataa ttctcctgta
    13021 aaggatatta cggttatcgg aaaaccattc aactggggat tctttttcct cggaagagat
    13081 cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca tggtcttgct tggattcgaa
    13141 gtactgatga tattaacgaa gaggaaaaaa gctctatcgc taatcggtgc atttgcgctg
    13201 acattttctc cagcagtcca gtggtggttc atgcaacatg tgggcgattt gattttcttt
    13261 actttgggac tgatgattgc tttttatcat tatttctaca atcatgataa aaaatggcta
    13321 cgaacgttga tgatgctttt ggtggttatt ttcggtattg ggtttatttt agtgatttac
    13381 ccagcccatc aagtgatgct ggcgtacttg ctgatttttt attttgttgg cctgcttatt
    13441 tactatagca agaaagtttc atgggatctg ttggatgtag tgttgattgg aggggcagtt
    13501 ttatttattg gcggagtcat ggttcatttc tggctaacgt caaaagacgc attgttagct
    13561 tctttgaata ccttatatcc aggaaaacgg gtaagtactg gaggaaagtg gacgattggc
    13621 gaattttttt ctttccttac aaattggaaa attccttttg aagatatcaa ctattcgaat
    13681 aactctgaag tggccctgtt ctatcacttt tttccaactg tattcctagc aagtccattt
    13741 gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg gacgtatttt gatgattttt
    13801 tgtctgtttt ctatcttttg gattaccgtt gggttgccaa aatggctagc agaaatcact
    13861 ctgctgtctt atgttcctcc attaagagcc atccttactt ttagttttgc ggcttgttta
    13921 ttatcgatct ggtttatcgc ttatatttgg agagaagatg ttattcctat ttggtataaa
    13981 ttcattttgt tggcagtaaa tgcatggtct tatttctatg cgattacgca ttcgaaaatg
    14041 gataattatt tttcggattt tgaaaccatc gcagttgctg tgttgggaac agtgatttta
    14101 gcttttgcat tatttggctg gagacgcttg ttttacctcg cttttgtcgc cttagttatc
    14161 gtatctggct ttttcgtcaa tccagtcgta gaaggtacag gagcgatttt tgaaaaaaca
    14221 ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg gtaaagtttg gttgtctgaa
    14281 gatgaattat ataacttcac cccaacattg ggagtgaaat ccttcaatac agtacgcttt
    14341 tatccagata tagctgcatg gaagaaaatc gatccgaaag aagaatacga gaagtattat
    14401 aatcggtatg ctcatacaag agcttttatc gctaaggaag atacaaagtt cattttagat
    14461 cgtccagata tgttttcggt cacgttgaat tttgaagacg cagaaaaatt agggatcgac
    14521 tatgtgatca gtaaacgacc attaactgat tacaaccaat tatatgaagc gcagttcacg
    14581 cagctatatg gaccggataa agacggttat atgattttta aagtagaata tccggattat
    14641 ccagatgtac cacaaacaca aactcaggaa aatcagctag tcgtacagta ataggctagc
    14701 tgagaataat cctgaaatat tagtagaaag gagagtgaag ataattcaag caatcgtaca
    14761 aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg ttgctgacgc tagtcttttt
    14821 tgtttggaca gcaggggtag gttttccagc aactttgttt atatataaca atagttcatt
    14881 acttatcgct tttagtgtgg ctattttgct cattttaaat gtttataaag caaaaggcgt
    14941 cgattttctt tatctaggtg ttgcgcttgc ccttttcatt ttcttttctt actttcggga
    15001 attaagaaat gagtctttat ttggagactt actgtttcct ttgattattt tattttgttt
    15061 agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt atttatctga ttgtctttac
    15121 ggtcttcctc ggaatcacgt tatatcgtgt atttacagag atccaagtac cagaaggaca
    15181 aagcatctgg gctccaggca attcgctttc tgatatttgg atcaatacga atacaatcgg
    15241 aagttctttg atgactcttg ctttattgat cagtggattt gccagttctt ttgaacgatg
    15301 gtatgttcga atcatcggtg ggatagcagt cgttgcagcc tttttaggta tttgggtctg
    15361 ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg atattggatc ttttacctaa
    15421 atcgttgatg cgtgtcattc gtgcgccatt tattgcctac acggttacga ttcttttagc
    15481 tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc aatttgttta ccggtcgaga
    15541 agatatttgg cataaattct atcagacgct aggagaaaaa tctgaacaag ttttactagg
    15601 aatgaaaaca tttatcttcc agcgaggaaa ccaattttta ggaaaccaca atagttataa
    15661 ttctattttg aatatctatg gattgatagg atttgggatt gctgctttgc tgcttatcct
    15721 ttttattggt cgcttaacgt tgaaagcaga tttatccaac gggcagatga cttttatctg
    15781 ggcctttttt gcggtgatga tgcagtcatt tatggaggat acattgactt catacccatg
    15841 ggttccgatt atttatatcc tgttggctat ggccgttcat cgatatgatg aaccagaagt
    15901 tacggaggaa ttctatgtta tggaagaggc atatgacgat atgacttatg atgatacatc
    15961 ttatgaggaa caaccagata gcgctcattc ttccaggatc caacgctatc attgataggc
    16021 acaaaatacg agagaggctg tgacaaccgt cgcagccttt tttggtttat tgcaattgtc
    16081 tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg tatcatagaa aattaagaaa
    16141 gaaataagaa cgcagctcgg taccaaaaat atggtaaaat ggcacttgac tgtaattcac
    16201 tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt atttatttat gaaggacatt
    16261 tatcagaaca gaaaattact gctacagttt tcgatcaatg acttcaaatc aagatatgca
    16321 ggatcgtttt taggaatctt atgggcgttt atcaatccgc tatttactgt attgatttat
    16381 tggctggtat ttggtgttgg actgaaagca caaatgatcg atgcaaaata cccatttatc
    16441 gtatatttag ttaccgggat gattccctgg ttcttcttct cagatgcatt tgcatcaact
    16501 acggttgttt ttcgggaata tacttattta gtaaagaaag tcgtattcaa tattcgtatc
    16561 ttgcccacga cgaaaatcct atccaatctg tacacccatt tattcttcat tctgattggc
    16621 atagctgtcg cgcttataaa tggtatatat ccttcagcaa tgtctttaca gctgatttat
    16681 tatttatttt gcatgtgtgc tttcttgaca ggattgactt ggctgacggc atcgatccaa
    16741 ccatttttac ctgatatcat gcaattaatt acgatcgtgc tgcagttgat catgtggaca
    16801 ttaccgatat tatggagccc taatcagttt gattcaggtc ttgtaagcat attgaaatta
    16861 aatccgctgt actatgttgt tcaaggttat cgtgagtcat ttttaagtga agcttggttc
    16921 tgggagcatg gaacatatac tttgtatttc tgggttttca cgttagtaat gttcgtaatc
    16981 ggctctgcag tgttccgtag attgaaacca catttttcag atgtgctgta aggagaatag
    17041 ctatgacaga atatgcaatt agactaaaag atatcacaaa atcttataat atgtacgcaa
    17101 aaccttcgga tcgatttcgt gaagccctca atccttttaa gaaatcctat catgatgtat
    17161 tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg tgagatgatc ggtttcgtgg
    17221 gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat tacgggagta ctgacaccaa
    17281 catccggcac gatggaaatc gaaggaaaaa tctcagcttt gctagaacta ggatcaggat
    17341 tcaatcctga gtattctggc tatgaaaata tctatttaaa tggaatggtt ctaggatttt
    17401 caagagaaga agtcgatgaa atggtcgatg atatcattga atttgctgac atcggcgacc
    17461 atatttatca acctgtaaaa acctattcaa gtgggatgtt cgttcgtcta gcttttgctg
    17521 tagcgatcaa tgtgaatcct gatattttga ttgtggatga agcattggca gtaggagatc
    17581 ttgaatttca actaaaatgt atggaaaaat ttacagaaat caagaactcg ggaaagacga
    17641 ttttattcgt ttctcatgat attagttcga ttcgccgttt ttgtgatcgt tcctattggt
    17701 tgaaaaatgg aaaagttgtc gaatatggtg atacgatgga agtaacgacg aattacgaaa
    17761 actttttgaa gaaaaaatca gttaaaacag tagatcgaaa taaaaaccaa gtagaacgct
    17821 tcgcagctcc agatatcgta gacgtgacga aagccgaatt gttggataaa gataaaaatc
    17881 cagtggatat gctggaacaa ggagaaaaac tatacgttaa agtaacctat gaagtaaaag
    17941 acgatacaat caaaaatcca gttttaggaa ttgctctgcg tacagtcgac aatttttatg
    18001 tgtgcggact caatactcta ctagatggta tcaagatccc ttggaaaaaa ggtgagaata
    18061 tcttttatct ggagtatgaa aagttaggtt tgttagctgg tgaatattat tttgacgttg
    18121 cggtctttga agaaaatgca acggttcctt tggtatacaa gacaaaatac atgaacttgt
    18181 ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt agaccataaa tgggaagaag
    18241 ggactcatag caatgaaata tgattttgaa atggacctag atgaacaaag cagtgtggga
    18301 aaaattgccg cacagatcaa accaggaagt aaagttttag agtttggtcc tggaaacggc
    18361 cgattaacga aacatttgat cggagcaaaa caatgtgaag tgagcattgt cgagctagat
    18421 aaggaattgt tcgattttgt cagcgagttt gcccaagatg gattttatgg agatatcgaa
    18481 agttttgaat gggcaaacta ttatgctgga caaacatttg attacgtttt gtttgccgat
    18541 gttttagaac atttggtaga tcctggaaaa acgttgaaga aagttagaga atttttgaat
    18601 gaagaaggag aaattctaat tacattccct aaccttgcgc ataattctgt catgattgat
    18661 ttgttcaata atcaattgcc atgggcttct tatggtttgc tagatgaaac ccataattct
    18721 ttttatactc atgatggctt ccaaaaagtt tttgaaaaag caggattata catcaatatt
    18781 gaagattatt tgtacttagc tgtaggtgat acagaattaa aatcaactta tgaagaattg
    18841 ccggaagctg tacgttatga tttcaaaatg cgaccatttg gcgaagtcta ccaatacttc
    18901 ttctcattga tgaaacatcc agtagcacaa agttccattg cgcagccgca aaattctaat
    18961 tatgtgaaag tattagaagt aatccagcaa acgaaacaag atgaaataat tcaacaaata
    19021 ccattcaata atttcacagg ggaaaatgag acactaagtt tccatgtatc agaattagca
    19081 gaacgaatgg tgtttcgctt cgcgcagcaa cctagcttta tcgaattcag cgcagaagct
    19141 gctggagaaa aactcacatt tatcgattca aatgctgtag tcaaaacagt aaatgattgt
    19201 tatttgtttg atggtaaaga tttaccagag ttctttttaa cggatattgc agggaaagaa
    19261 gtgactatcc attgtcacta tcgttttatc ggagaattga caccaacaat gaaagaattg
    19321 ctggaaacaa tccgtccaat ggctgaagtt acaaagcagc tttctgagaa aaatgatgaa
    19381 ttagaaagag aaaatcatta tcttattgag aataatacaa gattagatca gacgttgaaa
    19441 acaaccataa atcgctattg cacgttgttg gaaagtgatg aatggacaat caaacataga
    19501 ttagggcgac gtaagaaaga aacatcgaaa aaaatccaag aaaaagagct ttctctatgt
    19561 attgacgaaa aaatctggga tgcagaaaca aagatattga aaatcatcgg gtggggaatc
    19621 gctaattcgg atcgccaacc attgtcctat aagttatcag ccgaccaatc accatttttt
    19681 gaagcaattc ctgttttacg agaagaagtc aatcaagcaa aacagctagc tgaaggagca
    19741 aaagcaggat ttgaactgcg gatcctttgc gagaaggaac gttcattctt agttgaagca
    19801 gtggctcaaa atggtcaatc atggatcatt gaaatgtaaa taatgattaa cttataaaga
    19861 taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt atttatcgat caaatcactc
    19921 gtgaacgggc cactggcgat ttgcttattg tgggctgggc aattgatgaa gtgaccaaag
    19981 aaatccctac gataaaggta gaaaaagaaa atgtgattgc tgaagcaaca catgtcgtaa
    20041 gattggatat caatcattta tataatcttg atgttaagac ccaaagcggt tttaaggtac
    20101 gtttatcagg gaaaatgaga ggaaaagcaa tcttagactt ccaaacagca agacatcaaa
    20161 atggcatagc tgtgaaatta aacggtaaat atccttatga tgacggcatt gaatcttcat
    20221 gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta tgcaaggact catggcgtaa
    20281 aaaaagcaat ccgccgggtc aaactggaat tgaaaccagg atcgatcgac tatgcagagt
    20341 ggatcagtcg tcatgagaaa cctgatctga aagagcaaag gaaagaagta caaggatttg
    20401 cctaccgccc attgatttcc atacttatgc ctgtctataa cgttgaaatc aaatggctcg
    20461 aaaaatgcat tgattcggta cttgatcaaa cgtatgatca ctgggaacta tgtatcagtg
    20521 atgatgcttc tactgatcca gctatccgaa agtgtctaga atcctatcaa gcaaaagatg
    20581 atagaattaa agtcgttttc cgacaagaaa atggacacat cagtctggca actaattctg
    20641 cattgaaaat ggcagaagga gagtttatag cgctcttaga taatgatgac gaattacctc
    20701 catttgcctt atatgaagtg gcaaaagtgc taaacgtaca tccagaactg gatttgatct
    20761 acagtgacga agataaaatc gatgcagatg ggaatcgctt tgacccgcac ttcaaagcag
    20821 actggtctcc cgatacattg atgggaaata attatatctc tcacttaggc gtatatcgga
    20881 caagcattgt taaggaactt ggcggtttcc gtaaaggata tgaaggctct caagattatg
    20941 atttggttct tcgtgtgaca gaacaaattc ctgcagaaca catttaccac attgaccgtg
    21001 tcttgtatca ttggcggacg atcccgggat caactgcaag caatggagaa gctaaaagct
    21061 acatctatga ttctggagtg aaagcattga cagatgctct atctagaaga aatatcaagg
    21121 gaagcgtaca tcctggcaga atttctggtt tttatgaaat cacttatgat gttttgcaag
    21181 aagacttggt cagtgtgatc attccaacta agaatggcta tgaagatcta aaaacttgtg
    21241 tggattcgat catcgaaaaa acgtcttatc caaattatga aatcattatt gcagataatg
    21301 gaagtacaga tcctaaaatg caagaattat ttgcagaata caagcatcag ctgaaagacc
    21361 gatttatcgt cgaattaatt gacattccat tcaattattc acgtatcaac aatctggcag
    21421 cagaaaaagc aaacggcaaa tactttttat tcttgaataa cgatacagaa gtgattgaac
    21481 ctgactggat gacagcgatg gtttcttatg cacagtttga tcgaatcggc tgtgtaggag
    21541 caaaattatt ttatccggat gatacaacgc agcatgcagg tgtattatta gggatcggtg
    21601 gcgttgcagg acatgcattg aataactatg atcgaacaca ttgtggttac tttggccgat
    21661 tggtcattga cgtcaattac ttagcagtca ctgcagcttg tatgatggtc aaggcagctg
    21721 acttcaatgc tgtcaatgga tttgatgaaa tattagaagt agcattcaac gatgttgact
    21781 tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta tgcacatcaa gcagaattgt
    21841 accatttcga atctaaatca agaggttatg aagatacacc agaaaaacaa agacgttttg
    21901 ctggggaaat caaaaaaatg caagataagt ggccttctta tattgctcat gacccatttt
    21961 ataacgataa tctaactaaa gaaggaatcg gcgatttttc gttgagacct gattgattaa
    22021 aagaactgta ttttcatttt tattaatgaa aatacagttc ttttaattta att
//
LOCUS       DAFCNS010000049        21880 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFCNS010000049 DAFCNS010000000
VERSION     DAFCNS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.049"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            404..1444
                     /locus_tag="LNE23_001800"
     CDS             404..1444
                     /locus_tag="LNE23_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK6931653.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2268..2600
                     /locus_tag="LNE23_001801"
     CDS             2268..2600
                     /locus_tag="LNE23_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBK6931654.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3219..3560
                     /locus_tag="LNE23_001802"
     CDS             3219..3560
                     /locus_tag="LNE23_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBK6931655.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            3674..3913
                     /locus_tag="LNE23_001803"
     CDS             3674..3913
                     /locus_tag="LNE23_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931656.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            3980..4189
                     /locus_tag="LNE23_001804"
     CDS             3980..4189
                     /locus_tag="LNE23_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931657.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKRKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            4249..4920
                     /locus_tag="LNE23_001805"
     CDS             4249..4920
                     /locus_tag="LNE23_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBK6931658.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGVATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            4973..6949
                     /locus_tag="LNE23_001806"
     CDS             4973..6949
                     /locus_tag="LNE23_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK6931659.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDITQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITRLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKVSV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            6964..7716
                     /locus_tag="LNE23_001807"
     CDS             6964..7716
                     /locus_tag="LNE23_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6931660.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            7732..8487
                     /locus_tag="LNE23_001808"
     CDS             7732..8487
                     /locus_tag="LNE23_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931661.1"
                     /translation="MRNRKWLFVCAAALFTFTAYTQLTVADSQESTDSTEQTIQTSDS
                     SAIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            8500..8760
                     /locus_tag="LNE23_001809"
     CDS             8500..8760
                     /locus_tag="LNE23_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931662.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            8757..10847
                     /locus_tag="LNE23_001810"
     CDS             8757..10847
                     /locus_tag="LNE23_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK6931663.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            10874..11338
                     /locus_tag="LNE23_001811"
     CDS             10874..11338
                     /locus_tag="LNE23_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931664.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            11335..11577
                     /locus_tag="LNE23_001812"
     CDS             11335..11577
                     /locus_tag="LNE23_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBK6931665.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            11595..11897
                     /locus_tag="LNE23_001813"
     CDS             11595..11897
                     /locus_tag="LNE23_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931666.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            11967..14030
                     /locus_tag="LNE23_001814"
     CDS             11967..14030
                     /locus_tag="LNE23_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931667.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            14030..16642
                     /locus_tag="LNE23_001815"
     CDS             14030..16642
                     /locus_tag="LNE23_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBK6931668.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            16639..19170
                     /locus_tag="LNE23_001816"
     CDS             16639..19170
                     /locus_tag="LNE23_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931669.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            19220..19552
                     /locus_tag="LNE23_001817"
     CDS             19220..19552
                     /locus_tag="LNE23_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931670.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            19553..20146
                     /locus_tag="LNE23_001818"
     CDS             19553..20146
                     /locus_tag="LNE23_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931671.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            20309..>21880
                     /locus_tag="LNE23_001819"
     CDS             20309..>21880
                     /locus_tag="LNE23_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBK6931672.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQR"
BASE COUNT         8086 a         3348 c         4420 g         6026 t
ORIGIN      
        1 ctattgtttt ctagcctaaa gccttgtctt ataagaataa tataatatat actactgaaa
       61 taaggtagta tatattacta gtaatggtag tatgttttat atgcataagg tagttcttaa
      121 tttatttttt gttttgacac tggtagtact taattatctt taattttaaa actaaatttt
      181 attcatacgt ttttctggta gtatttaatt ataaataata tttatttaaa gtagttctta
      241 atgactttaa aggtagtaca taattcattt tatttctgat tttaggtagt atttaattaa
      301 aaaacagttt taatgcgttt gcatttttta tttatctttg ctattttcga aaagagtagg
      361 taattttaca taaataaaac aacgtgaaag tagaggatct acaatgactg attttaaatt
      421 ttttaaggcg gatagagttt acaacgaatt attttatcaa tttccaaaag tctttattgt
      481 ttctgacgaa tacaaaaaaa tgaaagattc aactaagatt gcctatatgc ttttaaaagc
      541 aagattagag atcgcaatca gcaaacggca aatcgatgaa gaaggtaatg tttattttac
      601 ttatacgaca aatgaactat gtagagtatt aaactgccaa aaacaaaaag cgatagcaat
      661 caaaaaagag ttggaatcct ttggtttatt attacaaaag cagatgggat ttaacaaaca
      721 gttagggaaa aataatccta atagactata tctagcagaa ttaaaagtct cagaaaatga
      781 tatctactta ctcgaaaaat ttgatagaga gaatagggaa aacgttgata aatcagaggg
      841 tatgaaaatc atacccaccc tcgacgaaaa atcagacgct gaatcccttg gggctcaaga
      901 gggtatgaaa atcataccgt gccaaaacgt tgataaatca gagggtatga aaatcatacc
      961 agaacttaat aataatatat tagacactaa tagacacaat atagacactg aaaaagaccg
     1021 cctacaagat caattgttgt tagacaattt tgagacaatt atgacaaacg acagcattgc
     1081 tacgtttgtc cctgaacgag tattaaattt gataaaaaca ttttcttcaa gttacagtga
     1141 agctcaaaaa accgtccaga ctattcataa tgcaaagaaa aaagctgaaa tagaaagtgg
     1201 tatttcgata gtttttgaag aactcgatag ttattatgtc aatgcagaac aagaattata
     1261 cacgacactg ttaaaagcct atcaaaaatt aaaaaccgaa aaagtcgaaa atatccagaa
     1321 cctgattttt gtctatgtaa aaaattggtt tatcgaaaaa ccaatagctg ctaaagtatc
     1381 aagtgaaaaa cgtttgaatt atgaaagctc cccaagcact attacgaaag actggttaga
     1441 gtgaaacttt actgacaaag tgattgttgc ttcatggagt aacattaaaa ttaaaaattt
     1501 tttattattc taattatatt acgaatatta caaaataata gctattttaa tagaatttat
     1561 aagttaaaat attatgataa aaatcacaaa cgtagccttt tacgtcataa gtgttaaaag
     1621 aaataattaa tttcaaatat agaaaaggga acgtatgttt gcatactcct tttttttgag
     1681 ttataataat acgcgaagag agataatttc attatttaaa caagctaaaa atcttgatat
     1741 tacttctttt caggcaatac gaatattatt cgcttacatt ttttgtataa atttaattgt
     1801 ctgctatgct tgtgatgaat tgattttcgt tctgcacttt tggatgataa atttggacaa
     1861 ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa ggctggtaac cttcgtcagc
     1921 gttcatacac ggcattaaga tttggccctt aatgcgttaa cctttcagtg ttacggtatt
     1981 acccgcaatg gatttagtat aacagatatt caaaagtgat accacttttt ttggctgtgt
     2041 taaggttaaa aatacgtgat attacgatga atctatgcct aagcacaatg ttataaaaat
     2101 gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta gagggaacgc atgtttggat
     2161 gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt ctctattttt gaaaaataac
     2221 aagaaaaact tgttaaaaat atttaaagaa attgggtggt ttattttatg tcatatacag
     2281 gaattttgag tctcaaagat atttgtcact acggtaaacg gtgtaccgct acagagaaaa
     2341 tcacgaagaa actatcaact ggccaaagta aaaccgttgt gcagtgtaag aaatatataa
     2401 tccagaaaga caaagtctca gaggaaatga tttattacat tgggaaacaa aagcaaataa
     2461 ttttaaaaga tcctatcccc ttaaaagagc tatatccaac aatcaaacat gtttatgacc
     2521 aaaacggtgt actgattggt cgaagaaaaa atggagtctt gcgttgtacc gcaaaaggga
     2581 tggggcgttt gatcggctga attgatcatt ctcataactt gtattttatc gtttttcgct
     2641 tgcttgaaaa tattgtcgag acttctaaag gaacttagag tcactagaaa aataaggagt
     2701 agacttgatc tatcaacaat tcccttcggg gagtgacggg gtaggtttta acgattggta
     2761 acgtaaaaaa ttggtcaatc gagtaaatgc gcttgtttgg atagtggtga aatgaatttg
     2821 tgtaaataat aagcgcagag taaacaagag ggaatttaga agttagacaa gttagaatag
     2881 cgtctagatt aatcatgagc agctactaag ggatctagac tttgacgcaa actcatggaa
     2941 taaggagtag gtcttctcgt aatggggagg acgcacaaga aattgtgtcg tgtgctgcag
     3001 tacatgtaga tacttgcgag gacgttgatt attggatcaa tcgagcattt accgctgtct
     3061 actagggttt ctcgtttcgg acgccataaa cgagtcgaaa ttttttatgt atttttaagt
     3121 aaacgaaaaa cgataaaata tgaacaagtc ctgaataaag tgaaaaaatt gatataaagc
     3181 atagagaaaa tgaaatttac gtaatggagg aatgacaaat gaaaaattat gatccgaata
     3241 tacgattggg cacacatact attaaggttt ctttccaacg ttgggattac aaaggatttg
     3301 tgacgtttag aaggggcgga aattgtaaag gattagatgt tttagcatta gacgaggatg
     3361 atttatacga tcaaacattg actgataatc caattggatt cggcttgcta cctgaagatg
     3421 atgaaggtaa tgaatggttt aaaatgacgc ttatgaatga taatggagac gaattatctg
     3481 ttgaagatac ttggtcatat ttgagtgatt atattgtcag ctttgaaatc attgaatttg
     3541 tagcggataa agaagaataa aaactggagg aataaatttt acagataaag gcttagaagg
     3601 agtcacgacg attaataaaa aggtagtatt accttaggca ttgagtaagg aatttattta
     3661 ggaggaatag tagatggcgc gaccagtttt agaggcgatg gatatggata ctttgacaaa
     3721 aggaaggtat gtacagctgt tagctcgtgg ttattcacct aaagaagtat tcaaagcctt
     3781 atctaaagga acagatatgg aaaaagaaag attgcgaaaa gaattcgatt attggagaac
     3841 ccataatgga atcaaggatt tgaaaccagc acggccagta attaaacgaa agaaaaagat
     3901 aacccaagac tgaagtgtag agcgtatgtg atcataggct tttttatttt tgcaaaaaac
     3961 aaggaaggta ggaaaactca tgaattatga agaaatcgaa aacagaaaaa aagtgtcgaa
     4021 agaaatggaa gaaaagttac taaaaacgat gaagcgaaaa caccttaaaa gattatctgt
     4081 cgcgcaatat atcaatgata tgcagttaac aggaaaagag aaagcttgtt tgttaggttc
     4141 aatgaaaaac tttgaacagt tgagacgaac ttatagaatt catttttaaa actttttcta
     4201 tggaaatgag aaaaagtttt tttgttttgt ctgaaaggag gcagatggat gaaaaaatgg
     4261 ttatttggtt ttttaggagt cactttaatc gtcttttgca gtgtgtttgg ctatgttagc
     4321 taccaaaaac atgaaggaga ggtgtttaag caaaatatag aaaaaaagat gcccgtagat
     4381 caaatcaatg cacatgcaaa gagttataag gaggatgcaa cgaacgtcaa taatgatatg
     4441 tctttggggc aaatgttgtc catacaaaaa gaagcaatcg aaatgggcgt caataaacaa
     4501 gtatttgcac aaattcagat tcctgcactt ggtttagcat tgccaatttt taaaggggca
     4561 aatcaataca ctttgagtct tggagtggct acttattttt atgaagatgc cgaaatgggc
     4621 aaaggaaact atgtcctagc aggtcataac atggaaatgc caggtgtttt attttctgat
     4681 attcagaagt tgtcattagg ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt
     4741 tacaaggtaa cgcgtaaatt tatcgtacct gagtacttta aattaatcga tggcgttcct
     4801 gaagagaact ctttcttgtc tttgccaaag aaaggagaga aacccttatt aacgttgttt
     4861 acttgtgtct atacgtcaca aggaaaagaa agatacgtgg tacagggaga actacaataa
     4921 caaggtttta atcaacgcga gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat
     4981 aaatcggtat gtaaaaaaag ctggaatgct tattggaatg ttaggaatca ttgcaggctg
     5041 ctttcaggga tatcgagcat atgcagaaga tatcacgcaa aaaacgccac cagagaaggt
     5101 caatatcacg gttcataaat taatgtacga tcaaggaacg caattgaatg ttgatattga
     5161 cgggatcaaa aacgatggtt atacgcacga tcaatatcct acaggtgtaa cgaaatacaa
     5221 caaggcagat tatggtgatg ttgagtttac gttaggaaat attacggatc aagtattacc
     5281 gactgaagat tctgatctaa ccaacgcgaa agtggacgaa atcgttaaag atgtagaaga
     5341 caagggatct aacagtgagt acgtcaagaa tgcaacaaac aaaattactt cagctgtaga
     5401 tgaaaatggt gagattactt ttgctgatca accagcctat gtcaattcca aagggaatgt
     5461 atatgtggtt tatgaaagta aatccgctgc tggattagtc acacaaaaag caaaaccaat
     5521 ggttgtgatt gcgccaatga ctgataatac aggttcaggt tttttaaaag acattcactt
     5581 atatcctaaa aatattgttt ctaagttgag ttttgaatta acgaaatttg gtgacgatgg
     5641 cacagctcaa agcaaacaaa cacctttaaa aggggcaaaa tttgagttat acaagggcga
     5701 acctggaaaa ggaacaaaat taggagatct tgtatcggat gatcaaggaa agctgactgc
     5761 tactgatttg acgttaggaa aatactattt tgtggaagtt ccttcagaag ttgtagtggg
     5821 atcagataaa gaaccaacag cagatcaata tttattaggc gcagacgctc gcaacgatgc
     5881 gcacaataaa ttaacctttg aaattacgaa tgatggcgtg acaagtgact taaaagcttc
     5941 atacgtaaac tataaagcac cagtgatcga taaaacagta acaaatggaa caggacaaga
     6001 acattctttc caaattggtg acgcagtaaa ttatcaagga acgattcata tcccaacaga
     6061 cattgcaggc ggagcagacg gtatcacagt aaacggagtg aaaagcgaaa cttctcctta
     6121 cagtgtattt aaatgggggg atacagcagg acaaggactt tcttatgtcg ctgctaaagc
     6181 aaatatcaaa gtaacaaata aagatggatc tgtggtatta aaagaaaata cagattataa
     6241 aatccaaaat tcagaaaatg gctttgtgat cgattttatc gtgaataatg gccaagtaag
     6301 tgatactgta gcaagtttac acggacaaga tcttcaaatg acttataaca tgtatgtaaa
     6361 tgacagcgca gctgttgcca atccattaac aaatagcgtg gatttcgttt ataacaacaa
     6421 tccatttaat caggaagaac accacgaaaa aacgaaagct gacgttgtga cttatggtgc
     6481 aaaattctta aaagtcgata gcggactatt tggaacagga atcaaagcaa caccgcttga
     6541 aggcgcagaa tttgctgcta aaaacgcaga aggaaaatat tatggtggat tagttgatac
     6601 ggataaagac ggtgtcaaag aagcagtatg ggtagacgat gtagcaaatg cagcgatcct
     6661 aaaatcagat aaagaaggtc actttgaaat cactcgttta actgaaggtg aatattcatt
     6721 ggaagaaaca aaagcgcctg aaaactatca aaaattaacg aaagagatct ctttcaaagt
     6781 agacaaagat tcatacaaag aagaaaaccg aatcacgatc aaaaacaatc aaaaagtttc
     6841 cgtaccaatg actggtagca acggttttca aacgtatgta ttaatctctt gcttgttgct
     6901 aggagcaggg gcactttctg cagttgttta ctttaagaag aaagcctaga aggatcaaaa
     6961 ccaatgatta tcaggaagcc taagaaaaag cgaatcatgg gtaaatggat cattgctttc
     7021 tggttacttt cagctgtagg tgtcttactt ttgatgcctg ctgaagcgag cgtggcgaaa
     7081 taccaacaaa atcagcagat tgccgcaatc gatcgaacag gaacggcagc agaaacggat
     7141 tcttctttgg atgttgctaa gattgagtta ggcgatcctg ttggtattct tacaattcca
     7201 agtatttctt taaaactacc aatttatgac ggaacgtccg acaaaatttt agaaaatggt
     7261 gtaggtatta cggaaggaac tggtgatatt acaggaggaa acggtaaaaa tcctttaatc
     7321 gcaggtcata gtggacttta taaagataat ctttttgatg atttaccttc agtaaaaaag
     7381 ggagaaaagt tttatatcaa agttgatggt gagcagcacg cttatcaaat cgatcggatt
     7441 gaggaagtgc aaaaagacga actacaaaga aactttgtca cttacttaga accaaatccc
     7501 aatgaagatc gagtaacgct aatgacatgt acgcctaagg ggatcaacac acaccgtttt
     7561 ctagtgtatg ggaagcgagt aacctttaca aaatcagaat taaaagatga agaaaataag
     7621 aaacaaaaat tatcatggaa atggctttta ggaagtactg tttttctttc agtcatgatc
     7681 attggttcgc tatttgttta taaaaagaaa aaataaagga gggtaaccaa catgagaaat
     7741 agaaaatggc tatttgtttg tgcagccgct ctttttactt tcactgccta cacacagttg
     7801 acggttgcag acagtcaaga aagcacggat agcacagaac aaacaatcca aacgtcagat
     7861 agcagcgcga ttcaaagcag cgatgaaacc gtcgattcgt caacagttga ttcttcagaa
     7921 gaacctaaaa ttgctgaaga cgatcaaata gtcaaacaga ccattcacgt tcaaaagatt
     7981 atttcttcag aacaattaga caaccagggg catgaaaaac aagaaggagt aaatggcgcg
     8041 aagttttctg tttatgatgt atcggacatt ttacaaaaga tggatgttaa ggatctcact
     8101 actgaccaaa ttgaatccca attaaaaaat cgagtgaaaa aactatccag tgatcaattg
     8161 aagctagttt ctaatgaaga aacaaagaca attgatcaac aaacaggtgt ctttgaattc
     8221 tcggtagaag tacaggcaaa tcagaaacaa gcttattata ttgtcaatga atcaagtcct
     8281 gaaaacattt ctaatagcga ggatattttg ctattaacac ctgtttctga taaaaacggt
     8341 gaattcttaa aggatgtgtg gatctatcct aaatcagaag cttcgcaacc taaagaagaa
     8401 gtaaaaaaaa tcgtttctac tggcgtaaag aaaaacttct ttgagaattg ttgggatttt
     8461 gttactcact tgttttctag agattaagga agaaataaaa tgaaactgat caaattatta
     8521 ttattcaatt tttcttgttt aaccgcagtc tttttgtact gcggtttatt attttctaaa
     8581 ctatctattt tgccaagcta ccaactatac ctaatgagga ttttaaacat accagctgct
     8641 tccatgccgc tagatattat ggaaaagctc attaggaatc cattatttac tacgatcaat
     8701 ttgatcgtta ttgtcggaat ttcactagtc atttataaaa aagaggggat gaagggatga
     8761 attttaaaca ctcgtggaca agacggttaa aatatgtttt tcccataatg atgatgttag
     8821 gtacattgtt tgggttaaaa acagccaatg tctttgctga aaaagttatt gtcgatccgt
     8881 caaatccaat ccagaacgta aagaataatt ttattattcc aaaagatgtt cctaatgcaa
     8941 aaacaaatat tactgtaaac ggagcgtcaa aagtttacaa atatcatgtt gatgccaaca
     9001 aaggtggttt tacagacgac tacgtaggat tagtggaggg aaacaaaaat atacgctatc
     9061 cttctttcca aaaagaatac ggtgaaacaa accttgtttt agaaggcgta acaaccaaca
     9121 ccaaagtcaa tattgactat ggaaaaatcg gtacgtataa cgggaaaaaa gtcaatatca
     9181 agctagttct atctaatatt cacttatact cggatacgtt gccgtggaat ctactagata
     9241 acaactatac aaaaactcat tttcgagacg atggctataa aaatacgaat ggtgcaatga
     9301 gtaaatctaa aaaaagaaca gtactttgga tctcagacaa tcttttctca ggaattgttt
     9361 accattcaac acaaatgaat gtgcagctag tagccacata tgaggacggt agccccgttc
     9421 aattttcagg agatacattt atcagtttta actctttgaa ccctgcagga ggaaaaagca
     9481 cggatcttaa aggagaatat gcccactatg acaaaatgaa tacgacagat tggtatgtca
     9541 gaaaagatac ggttctttca gaatttaaat ctttctacaa caatctgaac gtggtaggtg
     9601 gccacccagg aggaagctct aagctgacac aagcggataa tgatttcaat aatttgcatg
     9661 ataaattagg agatccgaag tttggtcaag gtactgtgtc ctttaaaata tctgaagcaa
     9721 atccgacgtt tgtgattggt tcttctaatg ttcaaacctg gtttacttta tcttctgcga
     9781 caatctttag tgttgttcct gatcaacctg aaaaaacagg ggttgataaa aatggaaata
     9841 acgtgaacga taaaatgttg caagtaggag acacgattca atatcgaatc aaacagaagg
     9901 tgaatcgatt aggagtggat ttactagcgg tttacgatcg atttgagtta atcgataatt
     9961 taccaaaaga agtaaattac gtcgatgcac acgttgaaag tggtacaaat aaaaaattcg
    10021 atgtatctgg tgaagtgacc tatgacaaaa caaaacacca ggtaaaatat gcagcgaaag
    10081 ctgatacatt aaaaaatcga atgaagtata acggcgaaac ttacgaatta gtgatcaatg
    10141 taaaagtcaa tgaattggcc aatcaaaata gtgtagccaa aaatcaggga acgtcgatca
    10201 ttaataaagt agaaaaggat acaaatatca ttaccgttta tttccctaaa attccagtga
    10261 aagaagtaca acaaaatgga aaagatgtaa acggacgaaa tgacggtaag aagggtacgc
    10321 cgacagctcc tttaaacgca ggatctgaag tacaatatct agtcacacaa aaatggcaca
    10381 caaaaggcgt agacgctgcg tcagatcact acaaacaatt tagtattcaa gatccaattg
    10441 aagctcgttt gacatataaa gaaggttctg cgcaagtaat agataagagt aaaggaaaag
    10501 atattacttc tgaaggcaca ctaacctatg atagcaattc aagaacgctg aagtgggaag
    10561 cttccgctga cttcttaagt aacaatcttt tagatggacg agaaattcag ctgatattta
    10621 cagctaaaac tccattacaa tcagaaaaaa atattgataa ccaagccgta gtagcagtag
    10681 ataatgtttc taataaaaca aatgttgtga caattggggt tgatcctaac ttgccacagg
    10741 tcattgttcc taaaacaggt tctacacatt tagtaacgat ttcagcggtc agcttactat
    10801 tacttgtgtt agcaactttt agttatgcgg ttctgagata catttaaaat aaaaaaacga
    10861 aagaatgaat aaaatgaaaa agttaataat agtagttact tccttgattg ccatgattgt
    10921 tattttagtc ggatgtagta atgaaaaaaa taaacaaact aatcaagata atggcgtatt
    10981 gaaatcagga actatgtgga aagaagaagt aggtggtctt gtttataatt tgaaaattat
    11041 agacgagact acctgggaat actctgagag tgtatggcat ccagatcctg tacaaataac
    11101 agtcaagagg caaaaagact ataaaggttt agaaagatac aaaatagtag attcagctgg
    11161 tgttagggaa tttataaaca aatctgattc tttatttatt gttgttccct atgagaagaa
    11221 tggcgtaaaa aaaataattt ttcttggatc tagcaaggat gaaaagcgaa cgaaagagaa
    11281 attgattcac gatggatcac aatccaataa atataagtta caaaagacta gcgagtgatt
    11341 gctagtcttt ttatttgtcc tgaaggagga atcacagtgg caaaagctaa aacgccattt
    11401 gaaaaagccg cgtctaaaat cttagaacgc cgcggtgtat ctgaaacaca gtggaaacaa
    11461 gaagtccttg aaaaagcaca atttgaccta ttacggggaa aagacaagga cttagaagga
    11521 tatatctatc aacgtgaacg tgaaaagctt gttttcgatg aaattattaa acactaaaag
    11581 gagcgaacaa ttttatgttt gaatcaatta caatgaattt tttaacacaa ccgaaagtag
    11641 tagctgcggt tgatattttt agagcattac aaactgccgc aactagttta aaaaacaatc
    11701 tgatgatttt tgctgctgct gcggccgttg cttccattgt tattgggggt cttttcttcc
    11761 tatttggacg aagaggagct gaaaatggga agatgcacgt aggctatgta ttgatggggg
    11821 taggagttat tttagcagca gcttctattg tggtttcgtt atttggattg tttggacaaa
    11881 cacctcaagg attataagaa agtaaaaaag taggagtaag aagtagtcga tactttttac
    11941 tcctttttta tgaaaggaga atactcatgt actttacgat tgcaggcaat accaaaacag
    12001 ataaagagtt aataaaaaaa gcaacgaagg aagtaggaaa gcgtacttct tttttaccga
    12061 aaacaattaa atattctgtc attgaaacgg aagaacagct acatgtgtta gggaaagtac
    12121 tggaacacga aagtcgatct tctgttaagt tgactctcac gttacatgac acagaaggaa
    12181 aaatagagaa agctggtaca gaatacgttt ttggcccact ggattatcaa atttcagaag
    12241 gtttctatcc tacattgatg gatttaatcc gccttgatgt gtttaaagaa acggatgaaa
    12301 ctatgagtgt ggaagagtct tatcgaaacg aaaaactttt acaagtttta gaagaagagt
    12361 tagagagcga ccaaagaaaa gtgaatcaag ggagtgaagt cgaagtaaat gaagaagtac
    12421 catgggaacg accaatattt ccaaagaagg agtcaaaaga aaaagagaca gtctatgttg
    12481 acgaacaagc tgatattccg actcagaaat cgatatttga ggaagagaac gatgtgatgg
    12541 gtcaattgga ggtagatccc tttgaagaaa caatggagga tgaagagacg gccgaagtag
    12601 aagttgatcc agttgaagcg aatgaagagt caacatggac tgaaggggat tctcaggaat
    12661 atattgaacc acttatcagt gaagaagtag aaaaaaacga aatagagata caaaaggcaa
    12721 ctgtatttga ttatattttg gatcaatatc cgccagagca tgaatggatg aaagaaaaaa
    12781 acaaattatt tttaaatgag ctttattcga aatatcaatt accccaaacc tatcaagcat
    12841 ttttagagag taaagaagaa ttaatctcac agggtaaagg cacactttta gaacagttag
    12901 agtctgtcca atcaacaaat tggcatgagc ttgcacaaga agatttatta gccacctttg
    12961 aagaacgtca acaagaaaca gatcaagaaa ttaatgagta tgcgacggaa cagtcgtcta
    13021 attgggagaa agcaaaagag gaactaaata aagaagaaca gcgagtgatt gaagaagaag
    13081 tacaaaaaat taaagagcga tacgaaaaga aacgcgacaa tgcgtattcc ttgtgtgtag
    13141 caaagatcaa taattttact cagacgaatc aagaagcgtt atcgaaagaa aaagaaaact
    13201 tgttaaaaga acgtattaaa gaacgaaaag aagaatttta ccgaggtttg cgtacagata
    13261 agctaaagat tagcaaagaa ttaaatgaac aactgaataa gctatatcaa acaacgactg
    13321 aagttcttat ggaaaaatac gaaaagatcc agcaagaatt acaagtgcaa attccaaaat
    13381 ggaaaaaaga gtatgaaatt caacaacaag aattgaaaga acaagaagaa aaacaacggc
    13441 aaaaagaaaa agagcgcgaa caattagaac taaaaaaacg tgaacaggat cttgcggaac
    13501 aacaaatggc aatagaaaaa caacggttaa accaagaaaa ggaaaaagaa gcagctgctc
    13561 gtaaggaacg tcaagaacaa atggatacat tgcgacaagc tcagttaaat tatctagcac
    13621 aacctcaacc aacgccttca gtagctgctg ttcaaacccc aatgaacaac caaacaaatc
    13681 taactccttc accacagcgt tcctttaacg gatggatggt gggatgtatt gcaagtttaa
    13741 gcttgcttct aggtggagga acagtatttg cttttaatca tatcaatcaa tcttcacagg
    13801 ctgattctgt ggccacaacg acgtcaatca aggaagaagc ccaagaacaa gcaagaaaag
    13861 agtatgaaca acaattagaa gaattattga atcgtaccaa tcagtcaaca acagagtcaa
    13921 gcatggaaaa tagcaaagag agtgattcta gtaaggctaa agaaggatca agcgattcaa
    13981 ctgtacaatc aaacacttcc gaaagttcag aatcaaatga ggtgaaataa tgcgtgttag
    14041 agaaacaacg aagcgaaaag acaaacgatt caatttagaa aagaataggt atctttctca
    14101 tttctatcgc tatactccct caggagaaga gctaccagaa aaacatgaca aacgcatttc
    14161 gaaaaagatt ttttatagtg gacttgtata cctcccttta gagtatttag taacaagagt
    14221 accagaagtt ttcaaccgat tggaaattgt taagggaagc ttacaaggaa taaatctgtt
    14281 tcaattacag gaaatgccac aggtctttca ttttgattca gctggttttt ggaatccatt
    14341 tactcctaca actagtttgg gtacacgttt agcaatttcg acagtttgtg tggttccagc
    14401 tgtacttatg ggattgaaaa tggacatgtg gtttcgtcct ctagcagatg gacaaaaagg
    14461 ggataatcga ttaatgacct taaaagaaat ccaacagatt tatccgcaaa tacctgaaac
    14521 aaaaactact tttccaggtt atggaggtat tccaatcaca cattacaaaa aatattggta
    14581 cattcaaaca gatactgtta atacgtgtat tgttggtacc tctcgatcag ggaaaggtca
    14641 gattgaagtg ttagccacca ttgacaattt atctcgtgca gaaaaacaaa gttcaatggt
    14701 cgttaatgat ccaaaaacag aattatatgt tggtagtaaa gatgaactag aagctagagg
    14761 gtatgatgta tatgccttta atatcaccga tccactgcaa tcaatgagtg ataatccatt
    14821 ggcactcatc gtgaaatact ggaaacgagg agacatagat actgcaacac agttaaccaa
    14881 tactttcaca aatacgattt attatgatgc caacgcttca gataacaaat actttaacga
    14941 aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg ttagaacaag ctgatcaaac
    15001 aggagattac tccaaattga caccgaataa tctcgttgag cttctttctg agattggcgg
    15061 ttttaattat tatccagatc cagacaatga attcaaacag ttgaatgcct tagatgaatt
    15121 tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa tatggggcaa cgaaaattgg
    15181 atctgataaa gcgaagggaa atattttatc aacggcaatc acgggattga atccatttac
    15241 tctaacgaaa attgcaaaga tgacttctca atcaacgatt gattataaat cggttgggtt
    15301 tccaaaatac ttagatttga aattacatga aagtctttta aatcaacgag tcgtagtaga
    15361 attttatcat aagggaaaga aatttcatga agaacgtgtc aaagtaggct ataaaggatt
    15421 ttgtgaaatc aattttgatt gtcatttagt tgcaggtgat caagtttgtt taaaatacga
    15481 tcatcaagga aaagaatatg tggcttggta tgaattttct caaagagtat tgaaagacga
    15541 agagggtaat atcgtttatc ggaaaaaacg aggagaaacc catttgccag aattagaaaa
    15601 agaagccgtt ctaacattag atgaggagaa aagtaactta ttagtagagt ctattcggat
    15661 gcactatacc gataaaccaa tggctatttt tatgcttaca cctgattatg atccatctaa
    15721 ccatatcatt gttagtattt ttcttagtca gttatataaa gaattatcta cgcaatgtgt
    15781 taagactaaa ggagataaat gtcaccgcag gattcatttt attttagatg aatttgggaa
    15841 tatgccacca atggataata tggaaggaat catgacggtc acagctggcc gaaacatgtt
    15901 gtgggatctg tttattcaat cttatcagca actttatgcg aagtatggag aacaaaacgc
    15961 agcgattatc aaagaaaatt gtcagacaca tatttatatt atgtcgacag atgataatac
    16021 catcgaagaa ttttctaaaa aagtggggaa taagacggtt gaacaagaga acagtaccat
    16081 caattcatta ggtatgaata ctcatatcaa tcgcaatgtt gattcagatc gtatcttaac
    16141 tcctgaaaga atttctactt ttttagaagg tgaaacgatt gtactcgctc ctttaaaacg
    16201 acaagatttg aaaggtcata aaatacggcc gtatcctatt tttaatcatg gggaaacgaa
    16261 tcttccttat gcttatcaat atattgaaga tttcaatcca tcgtgggata tcaatgatat
    16321 agacatacat tgtacacatg cggacttgga tcttaaggct ttacaaattg attatgatta
    16381 tttcctacca gatgaatatg ctagaaatct ctatcatgga ctgcatgatc aattagaaga
    16441 aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct cttgagaatg agacggctaa
    16501 aaaattgtta attcaatttt acgaagaaca agcagcagat cgacttcaag aatatgttga
    16561 aaagttgatc aaaaataaag aaatttctga aatgattggg aaacggttgt tattggaatc
    16621 tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt ctggcatagc cgactggtgg
    16681 tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg taacgaaagt cttacaagat
    16741 tatggtaatt atctgcagta tcaagatgta tttagttgga tttggaacac ttttctgtgg
    16801 ggattgatta aaggattata taatctcaat actttactag aaaaaaccat ttatcaaagc
    16861 tttgatttaa aagacttatt gaacgccgct ggggtaaatg cactatttca aagtttgatt
    16921 agtaaggtat tggctctttt ttgtgtcgtc acattaattt atttaggctt gaaatttttt
    16981 atgagtaaac acccaccaaa agtcaaaaat atcttggtta atttatttat ggccatgatc
    17041 ttgatttttg gtgggagttc tttaatcgat gaagggttaa atatcagtaa aagcttttac
    17101 ggagacgtca ctgcagcaaa taaagcagat aaaacgggtt cacctgcttt tcaaattatt
    17161 caaaataatg tgatcgatgt cagtacatta ctagcaactg acccgaataa agtagaatcc
    17221 ctgccaacag acaaaagaaa tgctttaaca gcgaaaaatt ttaaaacagc gaatatcaat
    17281 gggattatta ctcctaaaca agctaaagaa atggcggata aagataagag tggtttatca
    17341 gatttacaaa aggatcggtt aagagcgtta caataccgat tggaactaga tgataatggg
    17401 aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa aatatgtcta tacttcaggg
    17461 tatcgtcgat acagtagtaa accaggtatt atcttagctg gtgaatcttc tttggcggtg
    17521 gcctatttat ttatcctatt tacgatcgtt acttgcatta ttgagctggt atttaaaaaa
    17581 ttctatttag tgattgccgc gtcaacggac tttgagacag gccaacggat gaaaagtgcg
    17641 atagaagata tttctcaatc cttcttgtta ttggcgttta cgattttaga attacgaatc
    17701 tatacattaa tgctttcagg tatcggtgat ttgcatgcgg caggaaagat caatggattc
    17761 ttgtatgttg ttgctttaat tgtgctaact attgcgctat tcaaaggctc tcaatcggtt
    17821 acgaagattt tcggtgttga tacgagtctg aaaaacggca gtaattctct tcttagcatg
    17881 tttgccttag gaaatattgc gaaaaatgtg ggtagcggag ctaaaaacct tgcaagtgct
    17941 ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa atggatttgg ccatcgaaaa
    18001 gatgttaccc ctgataacgg ggaagaacaa tcagacgata aagctacaac tgtacctaat
    18061 agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa atggtgtttc taaagccgca
    18121 gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa cagctgaaga tcttacagaa
    18181 aaagccaaag acaccttaga agagaaatta gacggcacga gagctaaaga attggctgaa
    18241 gctgtgaaga atccatccga agcactagaa aaacaaaaag aaaaagcttc agaagtcaaa
    18301 gaaggactaa aagatgcgta tggtgaagga caagttaatg cagcagtaaa agctaataca
    18361 acaaccgaaa aagaaaatca agaaggtata gtgattcctt cagtcaaaga tcaccaagaa
    18421 gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt ccatagaaga aagaaattca
    18481 gaagaaggaa aaaatggaca gttaccaaaa actgatccag aacaatatcg ctataaacct
    18541 aacagtgacc ttgaaaatga gaagcgaatt tcagatttat caaaagtaaa tcaagaagac
    18601 aaagcagaaa atgagggaca gttaccaaaa actgatccag aacaatatcg gtatagatct
    18661 gttaactccg aaaatgaaaa acgcatttca gatttaccaa aacgaaatca agaagtacaa
    18721 acggatgagg attccatcgt tctgccaaat gataatggaa tcgaagttgg aactgaaaaa
    18781 tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa tgaaagatag tcgtttgaat
    18841 caattcaagg agaatgatca aagctttgtt caccaatcaa ctgcaggtac tgttcaaatg
    18901 aaaccaagtc gtttgcctga aaatggaaat ctgacaactg tttcttacaa tttatctgaa
    18961 agtggtgaga cagggaatgc gtccgttcaa aatgttgtga caagagaagc tgcaccaaaa
    19021 cctattcaaa acgttcatac aggttacaag ccagcaaatg tggcatttac tgcaccaaca
    19081 aaaataaatg tgacaagaga aactaaaaac aggaacaatg cattgaaaga acaactattg
    19141 agggatatca aacaggatat caaaccataa aaggcctaca agttaggcct tttatttata
    19201 tgaaaaggag aaatcaatta tggataaata tataaacatg aaaaaccctc caagatttag
    19261 tggagaatgg aagtttttcg cttttttctt tttagatcta ttaacgcttg gtggctgctt
    19321 tttttgggcg aaaatggtca ataatcttgt ccacttacca ctcttttttg cactcttgaa
    19381 ttatggctta tggattgcga tagcgttagt gattgtttgg cgaccctata ataacccagg
    19441 tcaacgacaa atttttgcga ttatcgagtc tatttttttt gcggacaaga accattatca
    19501 tgcgttagat cctaaccgaa attatcatgg tacgtataag gaggatgaat aaatgcgagt
    19561 caaaggacaa aattcagttg aaaggaaatc cattttgcag tttggaaaac cagaagagcg
    19621 ctctatcttg gacttatttc cgtataaatc ctgtacagaa gagggagtct taattactag
    19681 agaagatcat tttcaacgtt attatcgtgt agtttctaca gacgtggaag gattaaatga
    19741 acaagagaaa ctagaacgaa tgaatcagtt gaccaccgtc atgcgtacgt atgtgccaac
    19801 aattaagtta gttagtttga ccacagagac ggatctaacg gaacaaatcg tgcaaaagcg
    19861 gcaattatta gataaaaatc gtatggaaca gtccttagga agaaatctac aacgattaaa
    19921 aaaatatgaa aagaaattag tcgaagaaat ccaggaattg aaacgagcag aacaagaacg
    19981 tcctgactta agcttctttt tcgtcattga aggaaagaca atcaaggatt tacaaaccag
    20041 aaaccgtcag ttaatgcgat ctagtggcat tttagggtta aaaccactct acaaaaaaga
    20101 attgatcaag attctgtatc gaatgaacaa tatgaatgat gagtaggaat ggagaagaga
    20161 gaatgaaata tccgttgata tggttagaaa aagatgttcc gaaggaagag accactagaa
    20221 aatttcctca cactttaagc ctgggtaagt tgggaaaagg gcaatctttt aaccaagtaa
    20281 agaatcaaac aagaagaggt gactgtacat gttagaagca attaaaacag ctttttcacc
    20341 acaaaatgat caaaaagtac ttgatccatt aaagcaacta ggaaatcggg aaatagaaat
    20401 tttaaaatcc aaagggtatg atttagattt tttaaaacaa gtgatgccac aaggtgggtt
    20461 aaattttgat gaagaatatg tgacgtatgg taatggttat tcacgatgtg tgacagttta
    20521 tcactacaca aaaaatccta cgcttttttg gttggccaat ctcatgaaca atcctcatac
    20581 agtggccaca ctcgatgtta gcacagatga aaagcaaaaa gtgattgatc aaatcaatcg
    20641 tgccttagat gaattaaatg atcgatcaga taatgaacga aaagcgactg accgaaataa
    20701 aagcgcagaa gaatacctgg aactagcgga atatgctcgt aaattgactt ctaatgggga
    20761 aatttctaag caagtcaaag tgagagtaca tgtttacgga aacacgattc aagaagtaga
    20821 tgaacgagcg caggaactga tcgaaaatct aaaagggatg gattatcgtg cggtgactta
    20881 tctgcataaa accaaacgag attggcaatc gatgtttacc atgttcgatg aacaagctga
    20941 atggttcgga aatatcaaag ggcaatcact accatcgtta aacattggcg gaggtttccc
    21001 gttccaccac caaacgctaa aagatccagg agggacacaa tatggggaaa ccatgacagg
    21061 cggcccattt gtctggaatg cgacgtataa aaatagtgta cggttgtcta ctaatacctt
    21121 gcttctagga atgatgggag ctggtaaatc gaccgttctt aaaatgatcg cggaagccca
    21181 tttagctgca ggagacttta tttggggctt tgaaaaggga aaagacttta ttcctttctt
    21241 aaaggaatat aacggtatta tggtgcgttt agacggatct gacggaatga tcaatccttt
    21301 agagatattt gccacacgga cgtatgatga agcatccagt ttatatgacg atggatcagt
    21361 caaagatttg aagatcaatg aagcagcgtc ctatcaaaca cacttagaca aagtggtcta
    21421 tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg aaagcagaat ttaagaccta
    21481 cttaaatcgt ttttatgaag agtatggaac agtaccacgc ggctttactt cctcaaattc
    21541 gcgttcaaat acggagacgc aagtcacagg taaagatcca gaagcttatc cgacgttcaa
    21601 agaattttta gactttttag gacaactgga attaccaggt gcttcccaag agaaaaagaa
    21661 tcgaaaagaa gaaatggaat ccatcgtaga atccttatgt gagacgtatg gcatgatttt
    21721 tgatgggcat agtacgattc gacaccttga ccaacaacag ttggtctttt ttgacattga
    21781 ctctattaca ggcttagatc gaggggtaca acaatgtcaa atgtacatgg ctatgacgtt
    21841 gatttggaat caggcattga ttcaaggaag aagacaacga
//
LOCUS       DAFCNS010000050        21617 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFCNS010000050 DAFCNS010000000
VERSION     DAFCNS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.050"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..808
                     /locus_tag="LNE23_001820"
     CDS             59..808
                     /locus_tag="LNE23_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6931673.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="LNE23_001821"
     CDS             complement(1232..1402)
                     /locus_tag="LNE23_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6931674.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="LNE23_001822"
     CDS             complement(1596..1775)
                     /locus_tag="LNE23_001822"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931675.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1785..2009)
                     /locus_tag="LNE23_001823"
     CDS             complement(1785..2009)
                     /locus_tag="LNE23_001823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931676.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            2162..3274
                     /locus_tag="LNE23_001824"
     CDS             2162..3274
                     /locus_tag="LNE23_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6931677.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="LNE23_001825"
     CDS             complement(3352..3645)
                     /locus_tag="LNE23_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBK6931678.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="LNE23_001826"
     CDS             3813..4127
                     /locus_tag="LNE23_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBK6931679.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="LNE23_001827"
     CDS             4148..6127
                     /locus_tag="LNE23_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6931680.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="LNE23_001828"
     CDS             6140..6466
                     /locus_tag="LNE23_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBK6931681.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="LNE23_001829"
     CDS             6482..7000
                     /locus_tag="LNE23_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931682.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="LNE23_001830"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="LNE23_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK6931683.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="LNE23_001831"
     CDS             complement(8436..9941)
                     /locus_tag="LNE23_001831"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBK6931684.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="LNE23_001832"
     CDS             complement(10033..10623)
                     /locus_tag="LNE23_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6931685.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="LNE23_001833"
     CDS             10802..12448
                     /locus_tag="LNE23_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6931686.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="LNE23_001834"
     CDS             12497..13216
                     /locus_tag="LNE23_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK6931687.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="LNE23_001835"
     CDS             13695..14642
                     /locus_tag="LNE23_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6931688.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="LNE23_001836"
     CDS             14654..15574
                     /locus_tag="LNE23_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6931689.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="LNE23_001837"
     CDS             15615..17072
                     /locus_tag="LNE23_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6931690.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="LNE23_001838"
     CDS             17417..18031
                     /locus_tag="LNE23_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK6931691.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="LNE23_001839"
     CDS             18024..19748
                     /locus_tag="LNE23_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6931692.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="LNE23_001840"
     CDS             19745..21196
                     /locus_tag="LNE23_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6931693.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6975 a         3673 c         4364 g         6605 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagaaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tattttt
//
LOCUS       DAFCNS010000051        21564 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFCNS010000051 DAFCNS010000000
VERSION     DAFCNS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.051"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            81..842
                     /locus_tag="LNE23_001841"
     CDS             81..842
                     /locus_tag="LNE23_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBK6931694.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            1070..1264
                     /locus_tag="LNE23_001842"
     CDS             1070..1264
                     /locus_tag="LNE23_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931695.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            1533..1832
                     /locus_tag="LNE23_001843"
     CDS             1533..1832
                     /locus_tag="LNE23_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931696.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1929..2363)
                     /locus_tag="LNE23_001844"
     CDS             complement(1929..2363)
                     /locus_tag="LNE23_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6931697.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(2595..3305)
                     /locus_tag="LNE23_001845"
     CDS             complement(2595..3305)
                     /locus_tag="LNE23_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK6931698.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(3295..4155)
                     /locus_tag="LNE23_001846"
     CDS             complement(3295..4155)
                     /locus_tag="LNE23_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBK6931699.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(4160..4798)
                     /locus_tag="LNE23_001847"
     CDS             complement(4160..4798)
                     /locus_tag="LNE23_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBK6931700.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(4813..5112)
                     /locus_tag="LNE23_001848"
     CDS             complement(4813..5112)
                     /locus_tag="LNE23_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6931701.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(5155..6639)
                     /locus_tag="LNE23_001849"
     CDS             complement(5155..6639)
                     /locus_tag="LNE23_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK6931702.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(6659..7120)
                     /locus_tag="LNE23_001850"
     CDS             complement(6659..7120)
                     /locus_tag="LNE23_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6931703.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(7138..8205)
                     /gene="ulaG"
                     /locus_tag="LNE23_001851"
     CDS             complement(7138..8205)
                     /gene="ulaG"
                     /locus_tag="LNE23_001851"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBK6931704.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            8423..9193
                     /locus_tag="LNE23_001852"
     CDS             8423..9193
                     /locus_tag="LNE23_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6931705.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(9466..10294)
                     /locus_tag="LNE23_001853"
                     /pseudo
     CDS             complement(9466..10294)
                     /locus_tag="LNE23_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            10861..12096
                     /locus_tag="LNE23_001854"
     CDS             10861..12096
                     /locus_tag="LNE23_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK6931706.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(13095..13355)
                     /locus_tag="LNE23_001855"
     CDS             complement(13095..13355)
                     /locus_tag="LNE23_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK6931707.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(13382..14683)
                     /locus_tag="LNE23_001856"
     CDS             complement(13382..14683)
                     /locus_tag="LNE23_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6931708.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14975..15433)
                     /locus_tag="LNE23_001857"
     CDS             complement(14975..15433)
                     /locus_tag="LNE23_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6931709.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(15446..16776)
                     /locus_tag="LNE23_001858"
                     /pseudo
     CDS             complement(15446..16776)
                     /locus_tag="LNE23_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(16787..18229)
                     /locus_tag="LNE23_001859"
     CDS             complement(16787..18229)
                     /locus_tag="LNE23_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6931710.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(18216..18980)
                     /locus_tag="LNE23_001860"
     CDS             complement(18216..18980)
                     /locus_tag="LNE23_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBK6931711.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(19084..19959)
                     /locus_tag="LNE23_001861"
     CDS             complement(19084..19959)
                     /locus_tag="LNE23_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6931712.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(20214..20298)
                     /locus_tag="LNE23_001862"
     tRNA            complement(20214..20298)
                     /locus_tag="LNE23_001862"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20262..20264),aa:Leu,seq:caa)
     gene            complement(20316..20387)
                     /locus_tag="LNE23_001863"
     tRNA            complement(20316..20387)
                     /locus_tag="LNE23_001863"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20353..20355),aa:Gln,seq:ttg)
     gene            complement(20397..20469)
                     /locus_tag="LNE23_001864"
     tRNA            complement(20397..20469)
                     /locus_tag="LNE23_001864"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20434..20436),aa:His,seq:gtg)
     gene            complement(20531..20615)
                     /locus_tag="LNE23_001865"
     tRNA            complement(20531..20615)
                     /locus_tag="LNE23_001865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20579..20581),aa:Leu,seq:caa)
     gene            complement(20636..20706)
                     /locus_tag="LNE23_001866"
     tRNA            complement(20636..20706)
                     /locus_tag="LNE23_001866"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20672..20674),aa:Cys,seq:gca)
     gene            complement(20752..20823)
                     /locus_tag="LNE23_001867"
     tRNA            complement(20752..20823)
                     /locus_tag="LNE23_001867"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20789..20791),aa:Gln,seq:ttg)
     gene            complement(20835..20907)
                     /locus_tag="LNE23_001868"
     tRNA            complement(20835..20907)
                     /locus_tag="LNE23_001868"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20872..20874),aa:His,seq:gtg)
     gene            complement(20910..20983)
                     /locus_tag="LNE23_001869"
     tRNA            complement(20910..20983)
                     /locus_tag="LNE23_001869"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20949..20951),aa:Trp,seq:cca)
     gene            complement(20990..21070)
                     /locus_tag="LNE23_001870"
     tRNA            complement(20990..21070)
                     /locus_tag="LNE23_001870"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21034..21036),aa:Tyr,seq:gta)
     gene            complement(21072..21147)
                     /locus_tag="LNE23_001871"
     tRNA            complement(21072..21147)
                     /locus_tag="LNE23_001871"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21112..21114),aa:Phe,seq:gaa)
     gene            complement(21183..21255)
                     /locus_tag="LNE23_001872"
     tRNA            complement(21183..21255)
                     /locus_tag="LNE23_001872"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21220..21222),aa:Asp,seq:gtc)
     gene            complement(21265..21337)
                     /locus_tag="LNE23_001873"
     tRNA            complement(21265..21337)
                     /locus_tag="LNE23_001873"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21302..21304),aa:Val,seq:tac)
     gene            complement(21353..21424)
                     /locus_tag="LNE23_001874"
     tRNA            complement(21353..21424)
                     /locus_tag="LNE23_001874"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21389..21391),aa:Glu,seq:ttc)
     gene            complement(21439..21527)
                     /locus_tag="LNE23_001875"
     tRNA            complement(21439..21527)
                     /locus_tag="LNE23_001875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21491..21493),aa:Ser,seq:gga)
BASE COUNT         6765 a         4471 c         3463 g         6865 t
ORIGIN      
        1 taatcaacaa agaaaatgtg atttttttca ctatatttta tcacatgaaa attcattaag
       61 aaagtaggaa tcgaagtatt atgcacacac cagaagaaat tatggaactc agtattcatc
      121 ttggaacaaa aaaaatacat aaacctatct tagctaaact gattttaggt tttatcggag
      181 gagccatgat ttctctaggt tatttggcat acgtacgagt ctctgcttct atcccagaat
      241 ctatggctag cttgactagt ttagttggtg cctcggtttt tcctatcggc ctgctggtca
      301 tccttcttgg cggtggtgaa ttgatcaccg gaaatatgat ggctgtggcg attgccttta
      361 tggataaaaa agtcacattc tcacagctgg ttaagaattg gtcagtgatt acttttgcaa
      421 atattattgg tgcaattttc gttgcctatt tctttggaca ctttgttggt ttaactcatt
      481 caggtgtcta tctcgaccaa gtgattcata ttgctgatca taaaatcgct gtttcttggt
      541 ggcaagcatt aggctccgga ataggttgta attggtttgt cggtttagcg ttatggttat
      601 catatggagc aaaagatgcg gccggcaaag tactaacgat ttggtttcca gtcatgattt
      661 ttgtggcaat cggtttccag catagtgtgg ccaactgttt tgtgatacct gctgccatat
      721 ttgaaggtca agcgacttgg ttagaatttc tctcgaattt tatccccgtt tatattggaa
      781 atatcatcgg cggagctatt tttgtttcag gtttttatta cgttagttat aagcatcatt
      841 aagattgaag gtaatgttca ttcgagcatt accttcaatc tctttatata ctatttatat
      901 tttaagaaca atagcctttt attatatata ttattcaaat tagttatatc gagtgaaaaa
      961 aactttcaaa ttgattcttt ttatttatca taaagatata gatacataat cattttcact
     1021 tacatatatt tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa tgtattatgt
     1081 agaagtaaaa acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca gtaacgaata
     1141 tcctttactt ggttcatgga aagaagctgc tcctttttcc aaaccatgtg ccataaaaat
     1201 aaagaacgag ctagaaaaag aactgacttg tggcaaagct gttgtagata tcatcgaaaa
     1261 gtaaatccaa tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa attgctaaga
     1321 ttatagaatt agaagaagaa gacgcagtgt tgacaattga aaaaaaataa atgtttgtat
     1381 accatatgat tatttcaata tgtatcctgg aattggagat ctcgtagagc tatatcaaga
     1441 tgaatcaaat atacttgtca ttcatcatga aatatagaga cttataaaat tgtttatttt
     1501 tataatgtat cagtatagaa aggaaataaa acatggatta tactgttgaa tttgacagtg
     1561 aactacttaa gcaatttcca caagaagaac atttcatgta taaagaagca atttcccaca
     1621 aagtgacaga gtatttggag cataattttt ttcgtataaa gccagtgcgt actcgttcgg
     1681 caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg
     1741 ctttaaaaaa aaatcatgtg ttcatttttt atattagttc gaatttgcaa aaaattgtat
     1801 ttgataaaga ggtgagtaaa aaaataaaat agctgagcat tggaggatag attgagtgat
     1861 actaagtcta tcctcttgtt tttctaaaga actaagctag aattttttct tttgtacgtt
     1921 attatatgtt atttgtcatt accaaaaagc accttttttc ttctagtatc tatgacataa
     1981 gaaataccac ccatttcctt tacttgaaca aatcctactg attcataaaa tttttttgtt
     2041 ttctcagtat catctgtcaa taattcgatt tggactggtc cataactagt gatcttctcc
     2101 aaagcttcat tcaaaagcac tttgcctatt ccttggcgct gatacctcgg atgaatgagg
     2161 atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc
     2221 ttcccttccc cattacgctt tatcagagat tcagattgtt caaatgctct agctaacctt
     2281 gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat ataattcaga aatctcatcg
     2341 atcataaggg aatcaatatt catttttttc ttcctttctt atacaattct ccctctaata
     2401 atacagttct ttgactattt aatctatcac atataactct cctgtaaaga ttctctcgat
     2461 tccttaaaat ctctctttcc ttttagaatg catccttttt gataaataaa aaggttgtga
     2521 taaattttct tcacaacctc tgtttctctc tatcaagtgt tatcttttaa ttttctaccc
     2581 ttaccctagc atctctattg cccgtagtat gcatactttc catgtttgcg aaaataatgt
     2641 ttatctagta aatattgcgg gatttcacat atattggggt tgatcagctc gttttctttg
     2701 gccatcagac aaatttcatc taagatcaaa ctgttttcta ctgctttcat aggtgaatcc
     2761 ccccatgtga acggtccatg tccatagaca agtactccag ggacttcgtt tggatctaat
     2821 tttctttcct taaacgtctc gacaatcaca ttccctgtat gaacttcgta agcttctctt
     2881 acttcttctt tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc atcagcatga
     2941 gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa
     3001 tgagtatgag taatagcagt tatatcttca aaagtttgat acaaaacaac atgtgtgggg
     3061 agatctgaag atggttttaa gctatcttct tctattatat ttcccgacaa atctgttacc
     3121 accatttgat ctgcttgcat gcattcatat ctgacaccgc ttggcttaat gacgatgact
     3181 cctagctcac ggtccacttc actcacattg ccccaagtca atttgactaa tccgtatcgc
     3241 ggcaatgcca gatttgcttc aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa
     3301 gacatcatag ccagcctcct tcaatttaga acttaagtag tcttttgcct gctggatttc
     3361 tcttcgaaag tcttggcttt tttcactcca catttcaatc agaaatgttc catcataatc
     3421 tagacgtttc aaatgtctta ataatccaag aaaatcaaca caaccgctac caaaaggaac
     3481 atcacgaaac tggcctgaat attcttttgt tacagggtaa gtatctttta agtggatagc
     3541 agtgatataa tcgatccctt tctcaagttc tcgagccgga ttattttccg gccatgcgga
     3601 caaattgcct aggtctggat atacttgtag aaaaggggaa tgaatctgtt ctttgatttc
     3661 taaaaatttt gaaatggagt tcataaatgg atcatccatg atttccacgg aaagaatgat
     3721 gccatattta gaagcttcct ttacgctttc ttttattcct tcgataaagt cttctctgga
     3781 attcattgat ttctcttcat aataaacatc gtatccagct atttggatta ctttgacgct
     3841 tagattatgc gccaaatgga ttgctttttt cattagttgt ttcgctgctt ttttcttttc
     3901 tggatcagca gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg aatggatacg
     3961 tacaccggag ctgaacatct cttctctcag ctcagctatc ttttcttcag accaatctaa
     4021 tcgtgctaac cgctcatccg tctcatcaat cgacatctcg ataaaatcga agtccatttc
     4081 ttttacaagg gaaaaacgtt ctttccatga aatatctttg ggtaatgctt tttcataaat
     4141 cccaatttga gccatttcct tacccccaga tacgaatgat ctcatctttg aagttttgtg
     4201 cggccttcat cggatcattt gctgctgtaa taccacgacc agtgataaag gtatacacat
     4261 cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc accagttaca gaaacattaa
     4321 agcctagttc aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag
     4381 ataacagcgc atcacggctt tgatggtaga ttacctgccg gataccgatg ttatgccaat
     4441 ccatggcttg ttcaaaagtc caattgccgt agagttctac ttgcaattca cctatttctt
     4501 tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat gactgtcatc cagtctgctc
     4561 ctgcttttgc aacattactc gccactgttc ctccagcgtc tgcacatttc gtatcagcga
     4621 ctagtttttt atctgggaac atactgcgaa tacagcgaat cgctttttgt ccttcctgta
     4681 agcaaagaat tgtgcccact tcaataatat ctactacatc accaactttc ataacatctc
     4741 ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg caaattcggc ctgctcatct
     4801 tagttccttc ctttacttgt ttttttggta ccactttgtt tcagcaaatt tttcactgat
     4861 ttctttggct gacatcacat ttttgatacc gataactgta acttcttttt tctctgcctg
     4921 agtaaacata ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc cagctgaact
     4981 ttttccctct gaaatggcac aatgatggat tttggtaatt ggtacgttta gttctttcat
     5041 tgctttttcc acacttctca tcatcatcaa gcttgttcct gatccatttg cacacgatac
     5101 taaaattctc atcttaatcc ctccattatg agtgttgtct aatcagattt tcttttatac
     5161 tctctgcttt tctgcatatg tgtcatagtc ttctacaatc aaaaaataac cctcaggatt
     5221 tttccgatat tgtaactgcg gaatagccaa taaaccaatg acaactacgg caattccagc
     5281 ataacctagg actttcatga ttactgtaaa caatggccaa gctgttgccc agtcgaacat
     5341 cccgatataa ccaccatatg ttgacatccc aatccatgta gcgaataatg ctgaaccgcc
     5401 tacttgtatg attcctgaaa gaaacgggaa caggcaagcg gctttgaatc cacctcggtt
     5461 attggcataa actgctataa cggcattatc aaagaacaac ggaatgaaac cggctatgac
     5521 gatcgttggt gatttcaata aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc
     5581 aaacaagaac ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc
     5641 aattcctgga acagctcctg gtaaaattgt gtttgagatt ccttggaaag attgagttag
     5701 ttcatcgacg aatgtccgaa cacctaattg caagatcgcc agatataccg caaaatttaa
     5761 agaagtcgtc ataatgtaga ataagaaact ctgtccttct tgcatgaatt gcgcttgaat
     5821 caaataatct ttgcctagta caatcagaat gatcccaaag aaaaatagca tgagtaacga
     5881 agtagcaacc atattctcat tgaaaatcga caagaatcct ggtaatttga tatcttcgat
     5941 ttttttgtct tgcttgattg atctattttt cttttcatcc cgctttttca tccactcagc
     6001 taattttgca aatataaaga ttccgaacat ttgctgatgt gccactgcaa atccggcacc
     6061 ttctgtcaat tcctgcgtga ttcctactgt taagttagaa gctactgccc aataacaacc
     6121 taaaatcagt cccataaaaa tcaatacttg tatcctacct agatcaggaa agcaaaacag
     6181 taaaatccaa aaagcggtag ctgcttgttg aacctgaacg tttccggtag tgaaaacgga
     6241 acgcagtttc gtatatttct gaaaacggac taataagata tttaaaacaa atgcaatcag
     6301 taataaaatc atcacatctc caaaggtacg gccaaatgtc tccattaatc cagcatccac
     6361 agcattttgt ccaaaataag gatctgtaac catggcatcc aaattgaacc gttcctttag
     6421 tccaaccaaa atcggccgaa aattattgac caaaccacca gagccaacag tcaaaataaa
     6481 gtaaccgacc gttgccttta agaatccagc gatgcattca tgaagcgggc gtttcagcaa
     6541 aatatagcct aataacacga taaaaccaat caggtatgct ggctgcgtca agatatttgt
     6601 tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt
     6661 aattatattc tgccatcaca acttggtaat catcgatgga gttcgtagag atgagacgct
     6721 caaccaatcc atctgtcatt aataattcgg ataatttttg gatattttct agatgttctt
     6781 ctggatcttt tgctgcaagt gtaaaaaaca aactagccgt tttctcctca tcctcccctt
     6841 cactttcaaa acggattggc tgtttcaact tggtaaatga aatagctgtt ccaaaaacac
     6901 catcactttt ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca atataaggac
     6961 cgtatttttc tacacaatgg atgatctcct ccacatacgt ttcatcaata atttccttat
     7021 cgatcaatac aagacagctt tcggcgatag cttcacgcca atccgtgatt tctttatctg
     7081 ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa gaaactcccc ttctttctta
     7141 taaaaacgac ggaaacggtg tatcattttc aatcgagaag acatcttcaa atcctcgata
     7201 aaaattaaac tcaagatcat ctttgtttgt cggatagatg aatttcccgc ctacttgcca
     7261 gatataaggt ttaaatccat acttcaggcg atcctttttg aatttccaaa tttcagtgat
     7321 ctcttttgaa tcagccataa aattagacca aatatcgtag tggacaggaa tcactacttt
     7381 tgcattcaat gactccgcca tgcgcagcat atcaacagaa gtaactttgt ctgttatccc
     7441 tcgtgggttt tcaccatagg cccctaaaca gacatcgatt ttgtgttcat taccatgttt
     7501 ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa agattccctc ctgatgtttc
     7561 gaataaatag tttactgcaa tttgatccat ctcctgaggc atttttcctt ttaatacctc
     7621 ttctggatct tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag ctactacatt
     7681 gatgtctttg attttgactg catcacctgg ctttactgta atcgtttttt cttcaggaat
     7741 tccccatttt tgccacgtgc gaaccacttc ttttggccca atgaatgttg cttcaggaca
     7801 attttgatga actgctgcgg ctgtatggat atctagatga tcggaatgga tatgtgtaac
     7861 gattaaagcg tctacatttt ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa
     7921 attaggctgc atattttgaa cgccactcat acgcatcatc tgatggcctt ttttcatcat
     7981 gccattccca tgactacgtt ttcccgtccc acaccataga tcacataaaa tattcgttcc
     8041 ttcatgactt ttcagccaaa ttcccgtaca gcccagccac cacatagcaa aagttccagg
     8101 atccactact tctgtttcga tttcttcatt caagtatgtg ccccactcag gaaatgtttc
     8161 tactaaccac ttttcttttg ttacttcatt gacagtttta ctcatttgct tgctcccccc
     8221 atatcgttgt tccaacttac aagtaaatat taatgtgtat cagcatccat ggaaaccctt
     8281 ttatatgacc gtttgttttt tatatgattt tctttcagta atcaatgatt gtttcagtat
     8341 aataaagaca gatatgaatg tttaaatgaa caactagtca aataaatcca tggatcaaga
     8401 tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa
     8461 agaaattttg gaactgcttc agcagactga acaactatca actttcgaat tagctgaatc
     8521 ccttgatgta tctattagta cgatacgaag agatttaaat ttattagaag aaaaaaacga
     8581 gatcatacgc aattacggtt attgttctta taattacaaa aaccagactg attttgatca
     8641 atctggtcct gaaaggatta aacaagcgat tgccagagaa gctagtaaat acattaacga
     8701 ttatgatacg ctgtttatca attctagctc gactgcgttg aaaacactgg attttcttca
     8761 atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga
     8821 taattgtagt tatattttaa ctggtggaga gatgcgcttt ccaaaagaag cactagtggg
     8881 cgacatcgca ttaaatacga tttcttcagt caatgcagac atttgtatca ttggctgtag
     8941 cggagtcgat gtagtacatg gggtcacaac aaagatatta aatgaatcaa cgatcaacga
     9001 aatgatggtc aacaagacaa tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg
     9061 actgacatca aagttcaaga ttgctgatat ttcagtcttt gattatttga tcactgataa
     9121 attttgttct gaaaaagtat tagaggagat cagaaatacg gggataaaaa ccattcaagt
     9181 agattcattt taagaaccag gacgtaaata tgtaaatagg attatcaaaa aaatgaataa
     9241 caaatttatc gataaatgaa aaacacgcta cgctcaaatt gataaaggaa tcccaagatg
     9301 tgtccatcca atcgatacca tcataatcta ttgcttcttt tataaccgtt atatgttgcc
     9361 taaataggaa tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt
     9421 tcgaataaac ggtattcaaa tcagaacact gatttcacaa ccactttatt tcaggtcgtt
     9481 ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc gctatttttt ttgcgtccat
     9541 atatctttct aaataatcat cttctcctaa ttgcttatgg caagaagcaa tttcataaag
     9601 caagtattcc aatgcataag tcgatttatg tttcttatta tactcaatac ccttctcaca
     9661 taattggatt gctttatcat aatcctcgat tttacggtaa taggtggcga tatcataata
     9721 tacaatgcag accatcagat tatcaaaatc agtctcactc aatattttaa gtgtacgctc
     9781 cacataaact ttcgcaaaat ctaattctcc ttttgacaag tagatcattg ccacgtttgc
     9841 agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt tccaacactt gattaaaata
     9901 aaataatgct gcatctggtt tttttcgcat aaagatgatc aagcagtcct tgataaaata
     9961 aaatttgatt taaaagatca gaattactta ctttttcctg atcgatcgtt tgtaaaattt
    10021 tataagattc ttggtatttt gatacagaat tcaatgattg aatctcattt atttttttcc
    10081 tgattttgtc ttcttctgtt tctactaata tatcggagat ctcgacattc agctttttgc
    10141 aaagcgcact taagatattg atgcctggcg acatattctt gttttcgata ttgctgacag
    10201 ttgcttgttc acaaatgcct tctgctaatt gtacttgtgt taaccccttt ttctttcggt
    10261 attcttttag taatgctcca tctactttaa tcaaaacaat ttccttccct ttaaaaaatt
    10321 atttctcctt tatatcatac acaaaaaaaa gttctaaaca accaaaaatg tttagaacag
    10381 aataaaaatt ttaattttaa aataatattc aaattaaaca actggcgtat ctgctccttg
    10441 atttcttttt gtgttatctg aataattatc gtttacagtt ataacacata gtgaaaattg
    10501 tcatggcgag aaagtctaag aatattttta aacataacta tttctttctt acgtgtgtat
    10561 aatgacgaca gcacacctat caattaatat aattatttta ttttttatgt tctcatgttt
    10621 atttttaatg acacaacttc tcgtatttat ctggcacaac atataaatcg atcaaaaaat
    10681 tgttgcgtgt ttttttgaaa attggagttt caacttgatt acaaaattta aatttagaag
    10741 ttgtgaaaac agcgagagac agtttaatat tcaaaatata cataggctac tcgaacgctg
    10801 tgttttttag tatactagta agaaacgaag ggatagactc attccaataa ggaggatttc
    10861 atgaagacaa ttctttttgc tcctgcagta ttcaatttag ctgagacaac acgaatgatt
    10921 gaggttgcca aagctatgaa agaccgttat tactgtgtat tctttggctt ttctaaaact
    10981 tttgctcctt tgatcacaga aagtggcttt gagtatcatt tactagcgcc gatccttacg
    11041 aaaaaacaag aagaacaaat cataaaattt gaccagttgc gtacacttaa aaaccctttt
    11101 accaaagaaa tggttgcctc tcgtatcact agtgaacaga gtctgattgc taagtttgag
    11161 cctcgactga ttgtgacagg ttcgaatgtg acaatttttt tatcggcccg caataaaaag
    11221 attcctttag tatatgttaa accctatgct ttgagtgaac ctttttttca atcagagcat
    11281 gcgctgatgc cccacgagtt acgtttcttc ggccctttaa gcaatcccct ttggcactgg
    11341 acaaaaaagc gtatagtaaa tacccgatgg atccccaatg ccttcaaaca cgctgcacaa
    11401 aaagaaatgc ttcctttacc aaaaacaagt ttacaaatga tggatgctga tctaaatctc
    11461 atcacaacgc ctaaactatt tttggaagaa gaacagctgc cggaaaatta tcgaatcgtt
    11521 ggtccaattt ttgctaagct cagtacttcc ttgcctgaag aagtgctaac ttttatacag
    11581 cgaaagcaac aagaaagaaa acgtattatc tatttcgcaa tgggtagttc aggcaatccg
    11641 cgtatgttac gcaaaatttt gttcttctta caaaaacaac cggatctagc ggttattgca
    11701 cctattgact atctgcttga tgacattcaa tctgactcag aagatctttt aataacagaa
    11761 tatctgtcta gtcatctttt gcatgaattc attgattttt cttttatcca cggcggggaa
    11821 gggaccgtcc aaactgcctg tgaatccggc aaaccgttta tcggtgtggg gatgcaattt
    11881 gagcaaagct gtaatattga ctattgtgta aaatacggaa acgcaatcgc cctcacaaaa
    11941 ccagtgactg aaagaaaact gcagattgct ctgcgcgaag tttcttcacc caagattcgt
    12001 cagcaggcac gcacgctgca agccgttttt ccaataaatg gtccacaaca agcaattcaa
    12061 gaaatcgaaa ccttcctgag caaccaccca atatagtttc aagtagaaaa agctttttta
    12121 atagagactc atcagtaatt tcgctattcg agacccagac agtgaaaata caaaaaaagt
    12181 aaatagtgga ttgaaaagtt agagttactt aaattacggt gtctaaaaaa cacaaaatca
    12241 tccttaaaat aaaagatctg acgcaatcca aatgagcata ggtagcgtct gccatttatt
    12301 cttagtcaaa ttctttgaca cctttctctg gttggatcat ctcaacgttt cttcaaaaga
    12361 aagaccataa taattaggca aatacatttt cttaaaatgt gtcatataga tctagaagca
    12421 tcgctttgta gagatttgtg ctgctagtcc cttcaacaat tactaaatca aatgtaggat
    12481 ttaccacttt atctttgata atcaacctcc ctcttctgtt gagacaatga tctcgcaatc
    12541 cgatgcatac tcctatataa actgccagca atccccacat gctgagtaga tcatgtcaga
    12601 atgttctatg tcgtcagcaa cgataaacta aaagtcacgt tcaccatgac tgattgctat
    12661 tcccagcgca attatttcta tacacgtttc atgatttgaa taaacactct catcatacat
    12721 attaccattc atacaccata ctgcagcccc tactatatgg ttgtaagctc ccaaatctat
    12781 atttgccttg atcactttgg tagtagatcg actaattctt aatccattgc tgataaaatt
    12841 ttcatttttg cctccttctt tctgccaaaa cagttaaaac aataataatc tttatgtaaa
    12901 aaagggctgt gacaaaagcc ttgtcacagt cccatattcc gaataaacgg tggtcaaaag
    12961 cttgcatctg cggtaataag ttcaaccaaa cgaaaaataa gccaacattt cacaaaaatt
    13021 taagaagcaa ttttcgtaaa tttcttctta tttcttgaag ctaaccactt ctgtcacaac
    13081 cacttttaga acatttaaag cttcgatgtg acaaaccatc tatgctgctc gatcataaaa
    13141 ttgatagcaa aaattccaca aagtacatat gtcccccatg aaaacaaaac attcatatgc
    13201 ggtacaatga tctttggaag aatcacgatc aaaaatgatg ccagtgccca aatccatcga
    13261 ttgatttgaa tatgatatgt agctagcata ttcaatataa ggtgactagc cgttaataaa
    13321 aagataaaaa caaaaaataa aatcagccaa tacattgttt cccaccttct aaaaaccaaa
    13381 cttagacagt ttggacttcg ttttctccac gactttgttc atctgacagc atctttttat
    13441 cataagcttt tacaaatgga taatagataa taaatgctat caatgtgttg atcatgatca
    13501 agataacaac acgccagtca tttcctgctg aaagataacc gccgattgga gctggtaatg
    13561 tccaagttgg caaaacagta aatctattga ccatttctaa tttgactgca aaataactga
    13621 taatagtcaa aactaaagga actaagttga acgggatgaa catgtaagga ttcaacatca
    13681 taggagctcc aaaaatcatg ggctcattga tattgaagat accaggaata atagaaaaac
    13741 gaccaacctc tttcaagaat tttgatttac agaagaatat aaacaaaaca gataccacga
    13801 tcgttgctcc tgcaccacca attgttaccg tccattgata aaactgttca ggagcgatat
    13861 agggtaattg ggtggcagct gtaccatctg ccaatgcctt tgcattctca tccaacatga
    13921 tcagccacat tggacgaaca acagccccta caatactcat gccatggata ccaaatgacc
    13981 acaacaaatg gatcaaaaac attggtagta aaacaccaag caaattattc cctaaaatat
    14041 tggttaaagg ctcgaataac gataataagg tagcgtttac atcaaagccc gcgacgcttc
    14101 gaatcagcca agtaattgtt aaaatgactg cacctggaat caatgcctca aaactacggg
    14161 caacagcagg tggcacttgt tctggcattt tgatagtaat atttcgattt ttaaagaaac
    14221 gatatacttc cactgcaaaa atcatagata agatccccgc aaacatccca gatgctccca
    14281 aagaactcat aggtaagacc cagccaagag gtgccttttc aggagttaaa acgtttacag
    14341 gaacttgtaa cataaagaac gttcctaaag ataatatacc acctgtcaca ctatctaatc
    14401 catatgattt agctagatcc gaacccataa caaaagttgc ataaatagcc atcaatccca
    14461 tcgtcatcct aaaaggtaaa acaatcgttg ccgaataagg ggcaataaat tcattccatc
    14521 caggaaacgg taaattgata aagaccataa aaaatgtccc aatcaaaatc aatgacaatg
    14581 ttgaaacaac cccattacga attgctaata aatgacgctg tgtaccaatt tttgccattg
    14641 gtgggacaag tttctcttcg acccatttca tcagcttttc cactaaaacc actccttttc
    14701 aataaaatat gaaccattca ttgcatgaca acgcaaagaa tgttcataac taattatata
    14761 attaagaaat tttatttcaa tatattctct taaataatca aatttatttt taagaatcct
    14821 gaaaataagt tttctaaatc ataggagcaa caaaaaaatt cgataattaa caaaaatcaa
    14881 aatgattaaa tctagtgatt gaaacttatc atttcaaaaa aattttgtaa attcacccat
    14941 aaatatcacc ctatttaact tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa
    15001 tctttacttc ttggttttgt tttaataaat ttccttcaaa aatgcctgga gaaactacaa
    15061 tgatcaccgt atctttttgt gcttgctcca acttcttcaa atctataata gctaacagtg
    15121 tcccttcttt tacctcctgg ttactttcaa cccgtatctg aaacggactt ccttcgagtt
    15181 caactgtatc taaacccata tggatcagta tttttctgcc agattcactt tcgatcgtta
    15241 tggcatgtcg ggtaggaaat atattatcga ttattccttt aactggcgca tggatatatc
    15301 catcatgatt cgttatagcg tatccaactc ctatcatttt tgaagaaaag acctcgtctt
    15361 ttactgctgt taatggaata atttgaccac tagctacagc ttttagaatg tttcttgttt
    15421 taaagaaatg cactttttat cacctctact ttaatgctgg ccaatatttt ttgtttactt
    15481 tgatcatttc atctagtatt ttttttgcga ctctggcatt tggtacggtt tgattcaatg
    15541 taaatgcttg taaagctttt tgataagaac cttcaaagta agcatctact agtaacttct
    15601 ctgctgcaac ttgtgcctcc atcaatcctt tatgaaaatc actgattggt tttcttagtg
    15661 taattgtctc cacaccagtt gcaccaacat atgccggtat ttcaacaact gcatctgacc
    15721 gtagattcgg aatcgcccct tgattaggaa cgattagcat aaagcgacga cgatcatcat
    15781 tcaacaagga aatagctata tcgacgatat attgtccatg ctccccaaaa ttaaagttca
    15841 atacttcttc cacaataccg cggcgtattt tatcagccat ttctttggta tttttctctc
    15901 gtccatccat gaccatatta gcacgtgtat actctttgtc agtatgctca acgaccatat
    15961 caggatataa gtaatactct aagtaattat tcgggatatt acttggaaaa tttttcgtga
    16021 tgactgacat catttgaaag gttttttgcc aagttttgtc tcctatatta aaatcggcga
    16081 cttgcatttc ttttgttatt agtttttcaa taatctctgg cataatttct cgttgcaatt
    16141 ctttatcata aatcgatgta taccagccaa agtggtttaa accataatac gtaacgatcc
    16201 aattcttacg atcataaccg taattcactg caatcgtttc ttcaattgaa atagtcatat
    16261 cacaggcatt aatcattttt acattcggat atttacgacg aacagcttca gaaacgatcg
    16321 actctggatt tgtataattc aaaatccatg ctttgggggc atagtcttgt atatgatcga
    16381 caatttccaa taaacctttg attgagcgca tcccataagc aaatcctccg agtccacacg
    16441 tttcctgtcc tactaatcca tgtttaagtg gaatcttttc gtccttttct cgcatctcca
    16501 atcctccaac acgaatttgt gagaaaacaa aatcgcatcc tgtaaaagca agctttggat
    16561 catccgttgc acgaatgcga atatttgaaa aacctttttt ttcagcataa atttaacaat
    16621 caagtacata tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg
    16681 caaccgtttt tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc
    16741 tccagctata gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata
    16801 atttattgaa ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt
    16861 tttcagttgg tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca
    16921 tggtgggatc cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat
    16981 tggcttttcc tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg
    17041 ctaaccctaa ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac
    17101 gtcctaatgt ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg
    17161 caaaaaagaa aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga
    17221 tgagataatc aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac
    17281 agacaatccc tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa
    17341 acatgaaact aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc
    17401 ttcccatgat tcctttgacc atctctttat tttctttata agctgcttta tagatagcta
    17461 gacatatagc tggtatacca aacatcatta ctacaaaaaa acctgataaa aacagtccag
    17521 cctgaggatc tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag
    17581 atgtatagct tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg
    17641 ggatcagcaa acgattcaaa aacataaata tccccacacc taatgccccc atatcaacga
    17701 gcagttttgc aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa
    17761 tagctgcgat tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat
    17821 aagaaaaaac tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta
    17881 aaccggaaat aattccacca aaaatcccca tattcacttc tgggtccatg atttctaagc
    17941 cttttttcaa ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat
    18001 ttttgacaaa accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg
    18061 catttccagc tgcaccaatt actgggatgt tcagcatatc atcagctgat aaacgcatga
    18121 tcaatcccgc taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca
    18181 catttgattt aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac
    18241 tcaatcaaag gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact
    18301 tggtgagtac gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct
    18361 gcgtgacaca tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa
    18421 tattcttcca cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg
    18481 aaacaaggct caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga
    18541 accggcaaat catatttttt tgctaatgta acaactacct cctggttttc atccaaagta
    18601 tgagtatgat gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga
    18661 atctgagcat tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa
    18721 taagataagg atctgaactc tccgctttcg ataagagtat cgacagtttt gagaagtggt
    18781 ttgccacacg tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt
    18841 ttcacagcgt gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct
    18901 cgctgataag catccataat cccataattc acaccatggc tatagccaaa atcatccgaa
    18961 ttaaaaatca cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca
    19021 aataatcata gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt
    19081 attttaatag attcgtgcta gatgaattgc caaatagatg atttcatttt cttgaatggc
    19141 aagttgaaat tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct
    19201 atgtttaaat gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat
    19261 caagcgttta accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt
    19321 atcataaacg tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac
    19381 tatttttaag ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt
    19441 gatgaatcct acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac
    19501 cacttctatt gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat
    19561 tgctgtgaca tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa
    19621 tgtaatatag atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac
    19681 ttcttccgca aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt
    19741 gatcttttct ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt
    19801 actcacttta gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc
    19861 atcgatcaat acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc
    19921 actatatgat aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca
    19981 atttttaaag acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa
    20041 tagtagcacc ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata
    20101 tcttttttac tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa
    20161 aaacaaaaaa aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc
    20221 ggccggggtc gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc
    20281 caattccgcc acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac
    20341 gcatgaggga gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg
    20401 aatgatggga atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc
    20461 cacattcgcc atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca
    20521 tcttagtcaa taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt
    20581 gagtccagcg cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg
    20641 gtaaccggat ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat
    20701 accgccatta tactattatc aatctacaaa ataaagaata aattatagaa actggggtag
    20761 ctggattcga accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc
    20821 ccaagaataa aaagggcgaa tgatgggaat cgaacccacg aatgccggag ccacaatccg
    20881 gtgcgttaac cacttcgcca cattcgccat ggcaggggca gcaggaattg aacccacact
    20941 aacggttttg gagaccgctg ttctaccttt aaactatgcc cctataaaat ggaggagagt
    21001 ggattcgaac cactgaaccc taaggaacgg atttacagtc cgtcgcgttt agccacttcg
    21061 ctactcctcc atggtggcgc gggacagaat cgaactgccg acacacggag cttcaatccg
    21121 ttgctctacc aactgagcta ccgcgccata ttttaaaatt attaattgaa taacaagaac
    21181 gacggttccg acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc
    21241 ctacaccacg gaaccagttc attatggagg ttaacgggat cgaaccgctg accctctgct
    21301 tgtaaggcag atgctctccc agctgagcta aacctccaac attcaattaa aatgacccgt
    21361 acgggattcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg
    21421 ggccaggtaa atttgctacg gagagtaagg gattcgaacc cttgagacag tttgcaccat
    21481 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    21541 ctcaatactc tttctcaaaa tgag
//
LOCUS       DAFCNS010000052        21524 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFCNS010000052 DAFCNS010000000
VERSION     DAFCNS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.052"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            205..906
                     /locus_tag="LNE23_001876"
     CDS             205..906
                     /locus_tag="LNE23_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK6931713.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1515..2531)
                     /gene="tsaD"
                     /locus_tag="LNE23_001877"
     CDS             complement(1515..2531)
                     /gene="tsaD"
                     /locus_tag="LNE23_001877"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBK6931714.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2528..2992)
                     /gene="rimI"
                     /locus_tag="LNE23_001878"
     CDS             complement(2528..2992)
                     /gene="rimI"
                     /locus_tag="LNE23_001878"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK6931715.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2999..3541)
                     /gene="rimI"
                     /locus_tag="LNE23_001879"
     CDS             complement(2999..3541)
                     /gene="rimI"
                     /locus_tag="LNE23_001879"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK6931716.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3525..4247)
                     /gene="tsaB"
                     /locus_tag="LNE23_001880"
     CDS             complement(3525..4247)
                     /gene="tsaB"
                     /locus_tag="LNE23_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBK6931717.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4438..5418)
                     /locus_tag="LNE23_001881"
     CDS             complement(4438..5418)
                     /locus_tag="LNE23_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6931718.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5442..6926)
                     /gene="galT"
                     /locus_tag="LNE23_001882"
     CDS             complement(5442..6926)
                     /gene="galT"
                     /locus_tag="LNE23_001882"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBK6931719.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6938..7927)
                     /gene="galE"
                     /locus_tag="LNE23_001883"
     CDS             complement(6938..7927)
                     /gene="galE"
                     /locus_tag="LNE23_001883"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBK6931720.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8175..8342
                     /locus_tag="LNE23_001884"
     CDS             8175..8342
                     /locus_tag="LNE23_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBK6931721.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8404..10215)
                     /locus_tag="LNE23_001885"
     CDS             complement(8404..10215)
                     /locus_tag="LNE23_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBK6931722.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10212..10577)
                     /locus_tag="LNE23_001886"
     CDS             complement(10212..10577)
                     /locus_tag="LNE23_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBK6931723.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10740..11135)
                     /locus_tag="LNE23_001887"
     CDS             complement(10740..11135)
                     /locus_tag="LNE23_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK6931724.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11153..12115)
                     /locus_tag="LNE23_001888"
     CDS             complement(11153..12115)
                     /locus_tag="LNE23_001888"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBK6931725.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12115..12327)
                     /locus_tag="LNE23_001889"
     CDS             complement(12115..12327)
                     /locus_tag="LNE23_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBK6931726.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12348..13046)
                     /locus_tag="LNE23_001890"
     CDS             complement(12348..13046)
                     /locus_tag="LNE23_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBK6931727.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13066..13608)
                     /locus_tag="LNE23_001891"
     CDS             complement(13066..13608)
                     /locus_tag="LNE23_001891"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBK6931728.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13740..14744)
                     /locus_tag="LNE23_001892"
     CDS             complement(13740..14744)
                     /locus_tag="LNE23_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK6931729.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14741..15730)
                     /locus_tag="LNE23_001893"
     CDS             complement(14741..15730)
                     /locus_tag="LNE23_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK6931730.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15727..16533)
                     /locus_tag="LNE23_001894"
     CDS             complement(15727..16533)
                     /locus_tag="LNE23_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931731.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16699..17655
                     /locus_tag="LNE23_001895"
     CDS             16699..17655
                     /locus_tag="LNE23_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6931732.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17732..18250)
                     /locus_tag="LNE23_001896"
     CDS             complement(17732..18250)
                     /locus_tag="LNE23_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK6931733.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18338..18487)
                     /gene="rpmG"
                     /locus_tag="LNE23_001897"
     CDS             complement(18338..18487)
                     /gene="rpmG"
                     /locus_tag="LNE23_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK6931734.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18715..19161
                     /locus_tag="LNE23_001898"
     CDS             18715..19161
                     /locus_tag="LNE23_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK6931735.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19356..21251)
                     /gene="asnB"
                     /locus_tag="LNE23_001899"
     CDS             complement(19356..21251)
                     /gene="asnB"
                     /locus_tag="LNE23_001899"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBK6931736.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6480 a         4561 c         3704 g         6779 t
ORIGIN      
        1 actatatgta ttattgttgt tttttataca caaatcattc gcttcaaaac aaattattca
       61 ataccattgt atatttttta cataataaca aattatattt gtaaagtatt aaaacttgtt
      121 tctaatgttg gtatgttaaa actttacaaa ttctttaaaa aaagtgacaa taaatatagt
      181 gaacaataag gagaggactt tttaatgcgt gttaataaaa tcgattttca cttttcaaaa
      241 ctcagatatc aacctgattt tgaacatttc tcggatgaag agttccaaca gatcaaggaa
      301 catactgttc ttcgttctta taaaaaagga cagatccttt tcgacgatgg tgatcccaga
      361 caacggttgt atatcttgac caaaggactc gttcgtttag aacgttatga tgaaagtgca
      421 acttattact actatgacta tgtgaataca aataaccttt ttcctatgtc aggaatgttt
      481 ggagaagaga cctacactta tacagctcaa gcgatgactg atattgaaac attctacctt
      541 cctgtttcct actatgaaaa attagcacga aaaaatcccg aacagttgat ctatatgata
      601 caaaaaattt ctaaagtagt tgaaatacat gaattgcgta tccaaatcgg attgacctct
      661 agcgcctttg accgggtaaa acaaaattta ttcattttga aagaagaatt gggcgtacca
      721 acaaaagatg gtcatatcac cattccttat ccgatcacct taaaagaatt agcagtgaat
      781 agcggcacga cacgtgaaac tgctggacaa gtaatcaaac aactacgtga ttccgggctg
      841 ttagattatg acaaaaagcg ttttactttc tgtaagacag ctactactcc agctatgaga
      901 gaataacgcg aacaaaaata agcagtgacc ctgataaata tcagggtcac tgcttatttt
      961 cctccctaaa acttctgaaa gatccggcag ttttagatca ctggacaaga ctcaaagatt
     1021 ttctatgtgt gtttcagtta tgtaatattc actgtttgca aaaaatactg ttagattaac
     1081 tagatgaagc gtcttgtcct catccgatct agattattcg atattcctcg acaataaacg
     1141 attactcagt gagggcaatc agaggaacgg ataatgataa ataagctgta aaaaatagca
     1201 aaataacaat aatgattaaa agtatgtaaa aaatagggct ttgacaaaaa cgtgtcacag
     1261 ccctatttcg attaaacggt gtgtacctgt caactcttcg gtcacaagtc ataatgttca
     1321 taaacatgag gagctgtttt ttgaaattgt tccttgtgaa tcagagttaa acgacaggct
     1381 tacgaactct tgattaaacg gcgttcaaaa gcttgcatac gcggtaataa gaacagccag
     1441 gtcaaaaaca tgagaagccg tttttcctgg gctgttctta ttacttgatg cagggcagct
     1501 ttttcacaac ctctttattc agataaaacc aagcttggat cagcattcaa ctgatagcta
     1561 gcaaaatttc ttttttgcat ttccacatgt gctgctgctc caatcattgc agcattatct
     1621 ccacaaagtc gaagaggggg aatcactagt tctacctcag ggagtttagc tgccaaagca
     1681 tcctgcaatc cttcacgcaa cccttgattg gccgctacac ctccagctat aatcagttgc
     1741 ttgactggat aattttgaca agctcgaagt gttttagtaa tcaatacatc gatcacactt
     1801 gcttgaaagc tagctgctaa atcatttttg tctagatctt ctccacgctg ttgtgcatta
     1861 tgcaccaaat tgataaatgc actttttaat ccgctaaaac tgaaatcata attatcttca
     1921 tggatcatcg ctcgcggaaa atggtaatta tcttttccct gatgagctag ctgatcgatt
     1981 tcttttccgc ttgggtaaga aagacctaat actcttccaa ctttgtcata agcttctcct
     2041 gctgcgtcat ctcttgtctc cccaatgatt tcatatgatc catcttcttg catatagact
     2101 aattctgtat gtcctccgct aacaagaagt gccatcaatg gaaactggaa tggtttaacc
     2161 agacgggctg catagatatg ccctgccata tggtttacag gaataagcgg caattggtgt
     2221 gcccaagcaa aagccttagc tgcagaaata ccaatcagca acgagccgac aagtcctggt
     2281 ccataagtga cagcgactgc cgataaatct tctgccgaca caccagcttc taccagtgca
     2341 tcttccaagc ataaggtgat ttgttctacg tgatggcggc tagctacttc aggtactacg
     2401 ccaccaaaac gtttatgact gtttatttga gaagcaacga tgttagataa gatctctgtt
     2461 ccatttctaa cgaccgctac actcgtttca tcacaacttg attcaatcgc cagaatcaat
     2521 tcttcattca tttcatcgtc tccgttttta actttctgct cataatgatc gcatcttccg
     2581 tcggattgcg ataatatgat ttccgtttac ctaatatacg aaacttttct gattcataaa
     2641 gttttcttgc tgcttcatta gatagacgta cttctaaaaa aacatgcagc gaatctttta
     2701 tttgttccgt aatcagtaat tgtaacaaat gccgtgcatg cccttttcct tgttctgaaa
     2761 ctgcaacagc aatatttgtg atctcgactt catctattac tttcgagtat ccaataaagc
     2821 cacagatctg gttatcttca gctaaaagca gataatcaga gtgaggctgt tggatgtctg
     2881 ccaaaaactg cattttcgtc caaggtgaac catgtggata agcattttcg ctaatcatcc
     2941 atacgcattc tgccaagtac gcttcatcaa aatctttttt tctgcaaatc aactaatctc
     3001 actcattttc taaaagtttt atcatatcca acgcatcttc ttttgtttca gtgtaatatc
     3061 tttttttgat cgttgttgct tgaaacccca atttccgata aagtctttgt gcatcttgat
     3121 tactcatacg cacttctaat gacatcgtct cacagcgatt cattatagca aagttctcga
     3181 tttcgtcaat aagcagcgaa ccgattcgtt tacgctgata tgccggtcga acggcaatat
     3241 tcgtgatatg gcaatctaaa ccgaacgctc ttgaaccgat aaaaccaatc attcttccat
     3301 tatcaaagat acctagatat agatgaggaa gcggtgactt caattctgat ataaaggcac
     3361 tttttgtcca tggcaattcg ccattataga cttctcgttc gacatccagc agctctttga
     3421 tatcttctgt agtcaattct cttagtacgt aacgacgatc gatttgttcg actgtttttt
     3481 ctgtataaat cattttatct tgaaaaaagc ttctggcata cgctctaaat tttttcaaca
     3541 taactttcgt ctccaggtgt gtgcgtctcc aaccattttt cttctgcttc tactcgtttt
     3601 aaatagcgag gaagaaaagc ttgtttctct gcaactggtt caactaaagc acctaattga
     3661 gccacagtta caccacttgg aatatcccat gctggaactg tattaatcat cgcactaggc
     3721 aatatttcct ttatttcaga gaagaatttg tgacaatcac tcccgacgaa ataaatagct
     3781 tcattcagct tttgtagttg tgtgaacaaa tcagaagcag aaatatgtac atcttcaagt
     3841 acagtttcta atctattttc tttccagcgg tatgccccag cgtatacatt ttttcttcga
     3901 gcatcaaaca tgggcacaat aatattatcc acaccgatgc aattagccgc gatggttttc
     3961 agactagata ctcctactag ctctttatct aatgtataag caatcgtttt tgctgtcgtt
     4021 acacctatac gaagccctgt ataagaaccg ggaccatcag aaaccgcgat tcggtcaatg
     4081 tcttttggac ttatttgaag atctgcaaac aattggtcga ttgcaggcat cagtgttaca
     4141 ctgtggttgc gtttgatatt tgtttgtatt tgtccaagaa tttgttcgtc ttctaccaca
     4201 ccgatcgcca aagtttggtt tgcagtatct attcctaatg taatcatata tttgatcctt
     4261 tctgctatga cattctcgcc ttccattgta gcatactttt ttattctgag gatgaaaata
     4321 ccaaaggcaa aactagtaca ataatacatg attatctgct ggcgggcaaa ttttgcccct
     4381 tcctgaaaca caaaaaagct gcggtatcac tccattttcc cacagctttt ccatctttta
     4441 tttcgttgat tctcgaataa tcaactctgt ttccacaata atctttcttg aaaccggtgc
     4501 tttggaatta cttaattgaa gaatcgtctc cactgctagc tctcccatcc attcggtata
     4561 aacttttaca gtagaaagcg agggcgttac atatttcgct acactgacat cattgaagcc
     4621 catcacagaa atatcttctg gtaccactaa acctttttct tgaatcgctt tcagtgcgcc
     4681 aatcgctaat gcatcattag ctgcaaaaag cgcatcagga acatgcggat tcgtttttaa
     4741 atattgcttc actgcttgat atcctgactc gacagaaaaa tctgcttcga taattggacc
     4801 ttcgtcgtag cctttttctg ccaaaacttg ttgaaatgcc ataattctgg gatcttttag
     4861 ccgctgatgg tttttcttcg tatattctgt tccagcaagc atagcgatat gttgcgcttt
     4921 atgggagaca aagtgttcaa tgactttttg aacacttgac ttaaagtcga caactagaga
     4981 gtcgattcct tgagaagtgc cgtcagaatc taccaaaagc aaggcatttt gggatccttt
     5041 catttctaat aactgagctt cgtcgaattt tcctagtgcg atcgttccat ctgccggaag
     5101 atcactaatg gaatcccacg tttctttcaa taaagtaacg tttgcttctt ctgccttttt
     5161 ttcgattcct aggcgaatag ataaataata aagatcttca agctcttccg tatcgtcgta
     5221 ccactggatc aggcgaagcg tttgcttagt atttgctgca tttttacgat gtttcgtata
     5281 atttaagcgt tctgctgctt caaaaatttt ttgctttgtg gctaagccaa cggaaagatc
     5341 aggatcatag ttgagaactc ttgaaacagt agcaggagaa actcctgcca actgcgcaat
     5401 gtctttaatt gttgccattt gtctctcctc cttttttacg tttataaaat cgcagtaaat
     5461 cgttcaaacg ctgcctgtcc tttttcatct cgcttgaata ctccagcgtc ttccagtaca
     5521 cgtgcaaaaa cctgacctac agatttttgt acgacatcgg tgaccgtatc tttcgtcaaa
     5581 tcaggatgtt ctttttttaa ttgttctgcc cacggacgat gatattcagc catctggttt
     5641 tcttctccta ataagaaatg ctctacttct actagttctg gtttcaaacg aggtggtaaa
     5701 acagcaagcc ccattacttc gatcaaccca atgttctcct ttttgatatg ttggacatct
     5761 ttatgtggat gaaaaatccc atcaggatgt tcttctgaaa cattgttgtc tcgaaggaca
     5821 aggtccaact caaagtaatt gtcccttctt cgtgcaattg gcgtaatcgt atggtgtggt
     5881 gtaccatctg ctgaaaaagc tctgatagat actgtttcat ccgaatatgc tttccatttt
     5941 tccaatactg agttagctgc ttcaataagc aagtttttct ctgttgattg caaacggatc
     6001 acagacatcg gccatttcac gactcctgcg atgacttgcg gataatcttc tagtttgata
     6061 agtttttcta tcggtgcttt tgccatagga aattcatgac gtcctgcctg ataatgatca
     6121 tgagaaagaa tcgatcctcc aacaattggt aagtcagcat tcgaaccaac aaagtaatga
     6181 ggcaagactt cagtaatccg aagcagtcta gcaaatgttt cctttgtgat tttcatcggg
     6241 cggtgttgtt ctgataaaat gatcgaatgt tcattgtaat aagcataagg ggaatattgg
     6301 aatccccagc tttcaccgtc tagattcatt cgaataatcc ggtgattggt gcgggctgga
     6361 tagttcaagc gacctctata tccttcgttt tccatgcaaa gcatgcaggt aggatagtct
     6421 acttttaccg cctcgcgttc tgctgcgatt tgttttggat ctttttctgg cttagataaa
     6481 ttgattgtga tttccagttc accatactcc gactctgccg gaaatttgat gttttttgcg
     6541 atggctcttg ttttgatgta gtcatttttc ttacttaaat taaagaaata ctcagttgct
     6601 tcctctgggc ttttcgaata gtgctgtgca aagaatgcat tgacgactga aggtggcggt
     6661 gtgagaaaat ccattaattg cgcttcaaaa atctcttgtt ctgctggcaa ttctgaaaga
     6721 acaccatttt ctcttgcctt agcgacaagc tgatccaata agtctacaga ttcttcctta
     6781 actggtctta cttcaatgct ttctagagca tcttctccta ccatccctaa aagacgattc
     6841 tgtaaatata agcggtccat ttccatccat ccgccagctt cgatcgcaag tgtggcaaaa
     6901 tcacaaatcg tttgactgat tgacatatct cattccccta ttcttcataa ccatttggat
     6961 ggctgacatg ccaatcccat gctgttttga taatcgcttt gatatctgtg tattcaggtt
     7021 tccagcccag aatctctctc gctttttcac ttgaagcgac taatcggcta ggatctccag
     7081 ctcttcttgg tgctatttct gctggaattt cttttcctgt cacttctcta gctgcttcta
     7141 gcatttcctt gactgagtat cctttgctgc tgcctaaatt gaagaagttg ctttcatttc
     7201 cttctttcaa gtattccaaa gctaaaatat gtgcagcaat caaatcttct acttgaacat
     7261 aatctctgat acatgttcca tctggtgtat cataatcgtc tccataaaca gccaaggctt
     7321 ttctttgacc aagagctact tgtaaaatga ttgggacaag atgcgtttct ggtgtatgat
     7381 cttctccgat agatgcatct gcttttgctc cggctacatt gaagtagcgc aaagcgacat
     7441 agcgcatgcc atatgcttgg tcacaccact tcatcatttt ttccatcatc aatttacttt
     7501 caccatacgg atttttagga ttagctggtg tatcttctgt gattggagac tctttcggct
     7561 ctccataagt tgctgcagta gaagagaata cgattttatt cacgttgaac tcgtgcatga
     7621 cttcaagtaa gatttgcata ccgtatacgt tgttgttgaa atacatcaat ggtttttcga
     7681 cagattctcc tactaaagag cttgcagcaa aatggattac tccttcaatc ggttcctttt
     7741 caaatacgga acgtagaaat tctttatccc ggatgtctcc ttcataaaaa tgagcatctg
     7801 gatgtactgc ttgtttgtga cctgtgagca aattatctac cacagctact tgatacccct
     7861 tttgaactag ctggtctaca gcgtgcgaac cgatataacc ggctccgcct agtactaaaa
     7921 ttgtcataaa aaacactcct ttgattagta aaattgtgtt tgttgattct atataattta
     7981 aaaaacgaag tttactttta tggtgaaaac aaacaaagta aaatttcgta aattcatatg
     8041 gtaaatttat aataaaacat ttagtaaaca ttttcaaggg ttttttgcaa aggtttacaa
     8101 acaaacgtaa aactagcctt tttttctctt ttgttccata atagatataa caaataataa
     8161 atgaggtgga ttcaatgaaa aaatttgtat ctggaatatt agtaggaagt ctggcaacag
     8221 ccgctgccgt tgctggtttg gttgcttctg tgaaaaaaac agtgatcgat ccaattgacg
     8281 aaaaagaagc aatgatcgaa gaaaaccgta aaaaagcaat gcgtaaacgc gtttctcgtt
     8341 aataattatt taaaaatacg catgaagact ttctaaaaaa cagataagtc atcatgcgta
     8401 tttttatttg tcagtcaata taagttgtgc tattttatct gcctgtctca gattccatgg
     8461 atctgtataa ggtctagcta ctgctgcagt tagccaatcc acaccttcaa caggtattcc
     8521 atagcaataa aagtcagact ggatttcccc tgaagcatca atcacgcgat tcgtacctcg
     8581 ctcaacatct atagcttctg aatgataact tcctgtatta tctgaaaatg aaaaagggcg
     8641 aacaagtttg ttttccttca aattttttat gacaggatta cttgaacgag aaaagcttgt
     8701 ttgtggcaat cgagcttcaa tcaaatagcg actgctgatt tctttatctt gtagagaaat
     8761 aacatagcgg ttgtgctttt gctgaatatc aaatggcggt tcgatcaaat gaaaaatacc
     8821 cgcatctatc agtgctgcta attctttcgt ccgaatgatt ggcggaccga tcgttaaaaa
     8881 gctatttaga tgagtaaacc atccccatag tatttctttg tattccttag cagaaaaaac
     8941 gtgccattca atagcctgcc ggattggatc acgccagtct tttaatgcat caaatgttga
     9001 agcaaatgct cctgtgcaat tgcctttttc cgcttctttt atctgccctt caataaatat
     9061 tcggctagct tctgaaaagg gctcattctg ataggcaaga ggagtttcta aatacgacca
     9121 atcccatcta taagggatta gtgctggatg ctttccgaga cactcctctg aagagaggga
     9181 gagaaaatca tgctcaaaat ttttctttgg aatcggcaag tgatattctt cgatgatttt
     9241 cttgtaataa aacaactcaa cgtctttttt cagatagtca aaaaatgttt cgccagtcaa
     9301 aaatcccgac tgatgccatt gctccaggtt ttcttttgtt agtaaccgtg gccatgccat
     9361 tgctccgcct tgcttttgat ttcttcctcg agggtaataa gggagccccc ttcttgatcc
     9421 actgtataca atcggttcct tgcctgatgg atgatagacc agttttccct tttcgttctc
     9481 gaagcgccct cctcttgata ctgtaaacaa tccgacataa tcaaaaaaac ttaagcctag
     9541 acctcttaaa aagacatttt ctttaggcgg tatcttcgaa agatctacat ctgcaggatt
     9601 agaaggaagc tgatacagta aaacattttt ctttgcgtag tctacccaca ctctttcttc
     9661 ttctgtctca tcattttccc agtgaccgct tgctaaaaca agctgatcga tagaatagct
     9721 ttgctgatct gtctgtaaaa gataaccatc ttttcttttg cttacttttt ttactaaaga
     9781 ataaacaaac tgaatagaag ctggaaattc ttcttgcaac tttgagaaaa accagcgttg
     9841 atagagtccg tataaacatc gagtagattg ctgatctttt tccagacgat tgatttcttc
     9901 tataattagt tgcttatttt tggttaccgt ctgttcgata taatctgtag caggtccttt
     9961 actccattga aacaagttgg gtccctgtgt taccaatcct ccactggaca atgtttcatc
    10021 tgtaaaaagc gtcacttgtt gagaaacaga attcattaat aactccggtg gctgattcaa
    10081 ccgccaaact cgacctccag gaccattggg atcgaaaaga aaaagttcaa gagaatgtct
    10141 gtcctgatta ttatggagaa tcctttctgc tacacttaat cctctaggtc cggcaccaat
    10201 gatccctatt ttcatttgca tgcctccatt tcgataaatt ttttcaataa aacagcatgg
    10261 ttctgatgtt cattttttgc accataaaca aaagtaatga ctggatgttc tttcataagt
    10321 tgactgagtt taaaaatagc ttgcttcttt tcttcttcga aaatcaattc ttgctggtat
    10381 ttctttgcaa atgaagcaaa tcgttctggt acatggtcaa aagttttgcg tgcttcatta
    10441 ctaggtgcaa tttctttcaa ccacagatct aactgcgctt tttcttttga aattcctcgt
    10501 ggccagaatc tatccacgag tattctatag ccatcctctt tttcagcatc aaggtaaaca
    10561 cgttttaatt tgatcatagt tgtttccttc tttctacctt ttcgcttagc ttatatatgt
    10621 agataatagc acaaaataaa aaaaatgaac ggcacaacta tccattgcgc cgttcatgtg
    10681 atcttggtca aaatctaatg attgattcgg tttttaaagc tgtcatacaa tcatctgttt
    10741 tatacttttt tacttttgat ttttccagac catccgtcat atccaccctt aagtaaataa
    10801 atctcttgat aacctgcttt acgtaattta tttgctgtac ggatgctcaa acttttcttt
    10861 tgatcgtaaa tatagacagg ctggtctttt cgaatagatc ctaatgaatc tttcaaaaca
    10921 gtgtaaggga tgtttcttgc acctaaaata tgcccagcat caaacacatc tttttcgcga
    10981 acatcgatta cttgtgcttt tctcattcct tctcgaaatt cttcttctgt caaagtcgtt
    11041 gctgaacgtt tggccattac tcgtagatag acttcccaaa ggatgattgc aagcacaatc
    11101 aatagcaaaa tgatattaat tacccataaa accatgtaat gcctccgttt ttttaagcaa
    11161 atttcaaggc taatgaggca gcaccaatga caccagcttc attaccaagc tctgctaatt
    11221 tgatttttgt tgattctctt acttgcggga aagtaaattc tttaaaatat ttctctacac
    11281 ggctacgtaa aaattctcct gctgccgata ctcctccacc taacacgata cttgacggat
    11341 ttaatgtatt ccccaagttt ccgcaagcta aacctaagta gaagcatacg cgatcaacaa
    11401 ccatcaatgc aaacggatcg tcttcttgag ccatttcaaa gatatcttta cttgttactt
    11461 cttctccgtt gtctagcatt gctttcagtt ttgaatcccc tgcgtattct tctgccaaga
    11521 aacgacctag gcgtaccaca cctgtagcgc tagatacagt ttctaaacat ccgcgtttac
    11581 cgcaagtaca ttcgaaccct tcaggatcca cagtgatatg tccgatttct cctccagctc
    11641 cgctcacgcc gtgaatcaaa tttccgccag caacgatacc tccgccaaca cctgttccta
    11701 gagtaacgaa tacaacgtct ggatcgtttt ctccagcacc tttccatcgt tcacctaaag
    11761 cagcaacatt cgcgtcatta tcgatagcga atttgattcc tgttcctgcc tcgatcagtt
    11821 ttttcacaaa ttgaacttct gtccagttta agttatacgc accaataacc gttccagctt
    11881 cgctgtccac gcttcctggg gtacccatgc cgattccgat aaagtcttcg gctttcatat
    11941 tatataaatt caaacgatga ttgattgatt caatgatttc aggaacgata tgcttaccat
    12001 catccaaaat attggtatcg atgctccatt tttgctgaat ctctccttct ggtgttaaaa
    12061 ttgcaaattt tgctgttgtt ccacctaaat caatcccaat aatcttcttg tccattacaa
    12121 atttcctttc tttcggcttt cttcaattcg gtgctctctt tttaatatgc gccacgctgt
    12181 catgtatgta tcatgatcaa tcaaacggct atcatacaga ttacgcaatt cgatagtcat
    12241 taattcaatg tcatagatgc gtgcacccac atagataaag atgccaaatt ttttgaacag
    12301 ctgctgtaca tcatataagc tctccatctt taagcacttc cttcatatta tacaagtaaa
    12361 ccatattttt ttaaacccag taaaatacat attacaaaaa gaaatacaaa aactagtgta
    12421 gaaagaatcc tttggtgtat cgaatagctt gtctgttgtt tcggtagacc caaaacattc
    12481 cctaacacta atccgccaat tagcccaccg acatgtcccc aaacatctac agaggtatcc
    12541 agaagaccaa aaacaaatgt caacacaata aataaaagaa aatgtctgac tagctgctgg
    12601 atcgctgggt tatgtttaaa atgaaaccct aagataaaca acgcaccgaa caaaccgaat
    12661 atcgaagtac ttgcaccacc agacagaaca ttcgcttcgt taaaagcaaa gcttgctgta
    12721 tttcccatta tcccgctgaa catataaatc aagaaaaaac gccaatggcc atagattgcc
    12781 tcgatttgct gccccataaa atacagtatc agtgagttca ttacgaaatg catcagtcca
    12841 aaatggatga agattggtgt cactaaacgc caccattcat tgaagtgaac aagataagga
    12901 ccatacattc cggcataata agggatctca agtcgcggaa ataattccat catgaggaag
    12961 accgctgtct gtatccctaa aaaagcatag gtccataacg gtcgtcttaa ccatgcacgc
    13021 aattttattt gctgttgata attcatgaaa acctcccatt tcattttagt caataaacaa
    13081 ttgttggatc ttctgatcaa agctctccgg ttgccattgt tcattcaatt gttcacgaaa
    13141 aacaaggcta catgtttttt tatgaaaatt atttaaatac cggtcgtaat aaccaccgcc
    13201 aaaaccgatt cgataaccgg ctctattgta gataatacct ggtacaatga ggagatcgat
    13261 catctccggc aaatagagct tctctgattg tggctcaaga acaccaaaac tcgaacgtac
    13321 aagaacggac tgttcatcca tttcataaaa cgccatctga cgtttcggca aagttttagg
    13381 tacaactatt ttcttttgtt ctttttctgc tcgttggata accggctgtg tatccagttc
    13441 aaattcattc gataaagtcg tccctatcac ttttgctgac ttccagtact cgctggcgaa
    13501 tagctgttta tagattcgct gttctttttc ctttttcttg ccacttttgg ccagattttt
    13561 cagcttttcg attcctttcc ggcgtaatct tactttcaat tggtccaaaa actcaccctt
    13621 ccttctctca taaaatggta taggaaaaaa agaaaaatag caatcgaact ttcaaataaa
    13681 tgcataaata gaataaaaag aagacctaga aatgattggc cttccttttt taatgaattt
    13741 caactgttca gcgcgtgttt ttgtctgata aaaagtgcat acagaaaata tggcgcacca
    13801 aaaagggcaa tcaaaattcc tgtaggtact tccccacttg gtagaatcac tctagcaacc
    13861 atatctgcac taagcagaaa aatggaaccg aacaaaccgc tcagaacaaa ggtccagtta
    13921 ttttctttac gtacgactag cttcgcagca tgaggagcta gtagtcctac aaaagataaa
    13981 ctacctgtca ccgctacact accgcttgcc aaagcaacag ctaacaacaa aaggattttt
    14041 tgacttttag ccacgttaag gcctaatcct tgcgcgcgtt cttgaccaaa tgatagcaat
    14101 acaatcctct gtgcataaag tcctcctagc ggaattaaga taaccgccca tggaagcaat
    14161 gcccaaacat aaggccagct cgcaccccag attgatcccg ctaaccatgc attcacaaat
    14221 ccataatttt ccttagataa ttggatcgtg catagcaatg ttaatgcaga aaggccggca
    14281 ttaacggcaa taccggacaa taagattcga ttcataccca aaaagccatt tcgatcatag
    14341 gccgcaagat agacacagca agcagctaaa acaccaccta gaaaggctac aactagcaat
    14401 agaaacggca gcgaatagtg cgaaaagaaa cctaaataaa gcatcactaa aaatcctgct
    14461 ccggcattaa tgcccaatat actagaatct gccaattcat ttcttgttac tccttgcaag
    14521 atataaccag aaacgccaag acaagctcct gctaataaac tgatcagtaa tcgaggaact
    14581 cgaaattctc taacaatcag cgccaaagaa gatgattctt tccctatgaa cgtggcaaaa
    14641 agctgatcca ggtaaataaa aggcgtacca atcattaaac tacctataga caggatgaca
    14701 agtacaaaaa gcaagaaata gaacgtccaa tatatttttt tcaccgttgt tccctcctta
    14761 cttgtatcag taaaaacgga acgccaatca atgcaatgac taatccaaaa ggtgtttcaa
    14821 aagggggatt gatcatcctc gctgtaaaat ccgccaacat aaccaatcca ccgccaacta
    14881 aaaaagagac aggtagaaca taacgatagt ccgttccaac cattccgcga acaactggcg
    14941 gaaccaacag cccaataaat gaaacaggtc cgactaacga aacggcaatg gctgctaaca
    15001 ctgcaactag acagaaaaca atcaaccgcg ttcgtttagg atgacttcca agagctaatg
    15061 catgtgtttc actcaaatga atcaaagata ttgcttttcc taatgaaaaa gaaaccaaaa
    15121 cagcaagtat atataaagga aacaatactg ctaattgttt ccatgtgata ttggcagctc
    15181 cgcctacaaa ccaaaaagca agatcctgct gcagattaaa gaattgggta agtgcttggc
    15241 tgatagcagt aaaaaaagaa ctaatagcta cgccagctaa aatcagctgt aaaggattca
    15301 tgcccatttt cgaaaactgc aaaaaaccaa atacgagcaa agttacagca aaagaacctg
    15361 ccaatgacat tagaaacaca gaaaaatcag aagctttagg ccaaaaagca aaactaaggg
    15421 ccattgccaa cgaagcaccc gcgttgattc ctagtaagcc tgaatctgct aaaggatttc
    15481 ttgtcactcc ttgcatcaat gcaccgctca atgcaaaaca actccctaca agaaatgctc
    15541 ccgccacacg aggcaaacga atatttcgta tgatttggtg actttgtttt gatctatcaa
    15601 aatcaaacaa cgcctgccaa aaatctgctt tttccacagt cgctgcgcca tttgaaagag
    15661 caacaaaaaa aataatcaaa agaacaaaac agaccaaaag caggagtcta tttttcttca
    15721 tagtcctcat aattgatcct cctgctcttt tggcaacaaa tcataggtta gaaaaactgg
    15781 tttatttgtt cttggaagcg tgacaatcgt tgcatcaatc tcaaatattt ttttcataca
    15841 ctcttttgtg atcacttctt caggtgtacc ttctgtgatc aacttaccgt ttttcattgc
    15901 taataaataa tctgaaaaac gtgaagcatg attaatgtca tgaatcgaca tcaagatcgt
    15961 tgtttgtttc atttgattga tttcagtcaa taactgtagg atctctaatt ggtgtgccgg
    16021 atctaaaaat gttgtcggtt catccaagat caagatatct gtttcctgag caagcgccat
    16081 agcgatccag acacgctggg tttgtccacc agacagcgtt gcgatatccc gcatacgaaa
    16141 tccagctaga cctgtagcat ccaacgccca ctcaattgct tcatgatctt gcgtgtccat
    16201 gccactgaac agtttttgat aagggtatcg tccgtaagat actacttctt ctactgtcat
    16261 acctgtaggg tgttcactag tttgtgctaa taatgcaatt ttttttgcaa gttcttttga
    16321 actgtatgta gagatatcag agttattgac aatgatcttt cctgatttca caggtagtat
    16381 acgggtaaaa cttttcaata aagtagattt tccacttcca tttggcccaa tcaaactagt
    16441 gattttccct tcaggaatcg taacagacat gttttcaatg atttttttgt tttcgtagcc
    16501 ggctgcaatg tcagctgctc ttaatctcgt catttcaccc atcctttcgc tgaaaatcat
    16561 tctcaattat gacgctgttt caacatttcg tcaagcgcta ctttcatatc tatagccaaa
    16621 tgaagcggtt gacagcgttc gaaatatcag gaataataac caatgagaaa gattctcaat
    16681 tatgttagga ggaaagaaat gaaattaaaa acaaaagtat tcacattatc tagcatattg
    16741 ctgctggctg gagtactgag cgcttgctcc ccctcttcag ataaagaaac agctacaact
    16801 gccaattcga cgacttcatc caatagtact catacagtca cagatacact tggacataaa
    16861 gtagagattc caaacgcgcc caaacggatc atcggcagtt acttagaaga ctatctgatt
    16921 gcactagggg aaaaacctat cgcccagtgg actgttggca gtggttccat tcaagattat
    16981 ctgcaagatg atttaaagga tgtcccaacg atttcttatg accttcctta tgagaaagtg
    17041 ttaagctttg agcctgattt gctgttgatt tcttcttccg ctactgtcga aggcgggaaa
    17101 tacgaacaat atagcaaaat cgcgccaact tatgttgtaa aaaacggaac agatgtcaca
    17161 tgggaagaac aactaaaaga tgtcggcaaa gctttggcaa aagaaaaaga ggcagaaaaa
    17221 atcatttccg attatcaaaa atccatcaag aaaactcgag aagaactagc agataaaatc
    17281 gagaacaaga cagctgcagt gttgtgggtg accaacaact cagcctttat ggtcagcaaa
    17341 aatcggtcaa gtggaagaat cgtatacgac gatctggaat ttggtgttcc tgatctagtg
    17401 gaagaggtct ccaaaaaagc cacttctgac tggtctgctg tttcctctga aaagctagct
    17461 gaattagatg ctgactacat catccttgtg aacagtgaca aagatgctgc catgttcaat
    17521 gaaccaagct ggaagaatct aaaagcagtg acagacaatc atgtactgca attcggacct
    17581 gaatctagtt ggctatataa cggacctatt gccagtcagg aaatggtcaa tgatatcaaa
    17641 gaaaaactaa attgatttta actaaaaaaa gcatcttcac gattagtcgt gagatgcttt
    17701 ttttcttatt aacaaactag acgcatagat tttattcttc ttttgaagca accctatttt
    17761 ttactctttc ccatttttga aaacgatggg catatagatt ttcttgatcc gttatcccct
    17821 catactcctc tataagggcg aagtctgaaa aatcaatatt cccaatatac gtatcgcctt
    17881 caaacgtgct atcgatcaat gttcgccaga tccgaccagt gtaaggaaga agttcttgaa
    17941 aaatccgact accacccgaa acatagatat cttctgagat cgtttgacta tacgacaaaa
    18001 gttcatcgat cgaatgcatt acttccgcat tttctttcgt gcgaaaattt ggttgcgttg
    18061 tcaacacgat gatatggcga taaggaagtg aaagatcccc cattccttca taggtctttc
    18121 ttcccatcac taaaattcga tccattgtat gttcgcgaaa gaaacgcata tcatttggca
    18181 atcgccatgg taacaaacca tttttgccga tcagaccatt tttatcttgt gcccacatgg
    18241 aaataaacat attatccgct ccttatgatt ttagatgttt tgagataaaa aaagtctcaa
    18301 ttctttgacc agcaaggaat tgagaacttt aaaaacatta ttttgtttca cggtgcaaag
    18361 taactttgcg ttcgcgtggg caatattttt gtttttccaa acgatcagga ttgttacgtt
    18421 tgtttttgct tgttagatag ttacgttctt tacaagaagt acactctaaa gtgatgttta
    18481 cgcgcatgtt tttccctccc atttttacaa gaatcctcta agatgactct cagacttgat
    18541 tatcttacca tgattttttc tagattgcta gtgtttttca cgattttgtt aagtaaaact
    18601 acttgacgat aaatacaacg gttttacttc tcaaacaaaa tctttttctt tttgtataaa
    18661 tacccttaca cttgtttata tagattatgt tttttaataa agggggatac taatatgcca
    18721 tggaacatga aggactttcc tgcatcaatg aaaaacttag ataaattgac aagaaaaaaa
    18781 gcaatcgaca tcgcaaatgc attattagat gaagggtacc ctgataaccg tgcgatccca
    18841 attgccattg atcaagcaaa aaaatggtat gaagatgcta gtgaatcaga aaaacgaact
    18901 ttcgaaaaag aaaaaaatcc aagcaagacg gatgaacacg atacgaatcc tcgtgctgga
    18961 aaactgcttg attcagatgt gatcgtagaa tatgaggaag aacaatggat cgtgaaatct
    19021 aaaggggcaa aaaaggcaag caaccatttt gatacaaaaa aagaagcaat cgaaaaagga
    19081 aagcaagtag cacaaaacaa agaatcaact ttgttcattt ataaaaaaga tgggacaaaa
    19141 gaaaaagaaa tcgattattg aacatatgta aaggggttgt gacgaaaatc gtgtcacaac
    19201 ccccttatgt tcgaaacaat ggtgttcctc tgccaactcc gaaccataag tcataatatc
    19261 tataaaacgg gaagagctgt tttttgaaat tgtttcttat cactcagtgt taaacggtag
    19321 gagaaaacct cttttttatt gatatttgat tttcatcaaa cagtcacata gtcgattgct
    19381 tctgcttgcg gaatcgtttc atctacaaag tagactttat accatgtcaa gaaactgaag
    19441 attgtccaga tttttctttg ctcttcttta cgtccttcgt gatggtcatc tagcaacttc
    19501 atgatttttt cttgatcaaa aaattctttt gcaaaatctg cttcgaataa tgctttgact
    19561 tgttcgtagc cgtgttcttc tttcaaccaa gctttgattg gcacagggaa tcctaatttc
    19621 acacgattcg accattcttc cggaagatgt ttgttcgcag cttgtctgaa aatatctttt
    19681 gtgttgtttt gattcaataa aaacttactt ggtatttgtt gagccaattt catgacttcg
    19741 atatccaata aaggtactcg gacttccaaa gaacttgcca tagaaagttt atctgccttt
    19801 aacaagatat cttttggcat ccattgatga agatcgacat attgcatctt attgacttca
    19861 tctttgattc cttctgtttt ctcgtaatga acagtcatga tttcatccac agatggtgct
    19921 gtttgatatt ttggtgttaa cacttctgat gcttctgatt cttcgaacac tcgagcatga
    19981 ccgataaaat attcttttgc aggagcaagt gattcatata aatggatccg tccatggaag
    20041 tttttcattt ttccgatttt acgtcctaaa ttatagcgtg ttccttttgg taattttttc
    20101 aatccttgag caatcacacg gatgaatttt gaattcgtat ggaatccata cgtttggtat
    20161 cccgcaaaca gttcatccgc cccttctcca gattggacta cccgcacact ttgtgaagca
    20221 agatttgcta aaaagtacaa aggtacgcaa gaaggatttg aatcaggttc atctaagtga
    20281 tactggatca aagggaatgc tttgaacgac atgtctccgt cgatcactgc tgctgtatta
    20341 tctaagtcca atagttcggt tagttttctg gcttcaatcg cttcattata ggttttatca
    20401 tcaaacccga ttgagaaaga atgatctggt ttcaatacag aggttacata actggaatca
    20461 accccgctgg ataagaaaga accaacttct acatcactga ccgtatgtgc ttcaatagaa
    20521 gcttgaacgg tttcgtcgat tttttcgatc cattcttgtc gggaatgcgt ttcttttgtt
    20581 tcaaaatctg catcccaata ttgctgaatg tctatttttc catctttata cgtataataa
    20641 tggccttcag gcaaacgata gacatctttg aagaatgttt cgccgtttaa tggagaatat
    20701 tggaaagtca tataaggttt caacgcttct ttgttcaatt ctttgttaaa atcaggatga
    20761 ggcagaaaac ttttgatttc tgaaccaaac ataaatgttc cgttcatttc tgcataataa
    20821 tatggtttga tcccaaagtg gtctcttgca ccgaataact cattcttttc attatcccaa
    20881 atagcaaaag caaacattcc gcggatcttt tgcaataatt ctgttcccca ttcttcgtaa
    20941 ccatgcaaaa ggacttctgt atctgcatgt gtttgaaaaa cgtggccagc tgcaatcaat
    21001 tcttctctca aaggctggta attatagatt tcgccattaa aaataatgat ctttgtccga
    21061 tcttcattgt aaataggctg agtaccgccc tctaagtcaa taatacttaa tcttctgaat
    21121 cctaaagcaa catgtttatc gatatattct cctgaactat taggtccacg gtgaacgatt
    21181 cgatccatca ttgtattaat aattgttttt ttgttgtcct tgtcgttaac aaaacctaca
    21241 ataccgcaca tttaactttc acatcctaat ataaaattac tgtttatttc attcatatgc
    21301 atcttatcat agaataaata gcggacgaat attttctctc ttacaatttc gtcacgtgaa
    21361 aacaaaagat gaaagtttct gtttttacag aaaataaagt cccaatatct aatttaccat
    21421 gttttctttt ttgaggctac cttattctct tcgtgttaaa aaaatattag tggatgctat
    21481 ttccaaaaaa accagcaaaa aaagacacga cctaaagatc gtgt
//
LOCUS       DAFCNS010000053        20802 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFCNS010000053 DAFCNS010000000
VERSION     DAFCNS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.053"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            319..627
                     /gene="rpsJ"
                     /locus_tag="LNE23_001900"
     CDS             319..627
                     /gene="rpsJ"
                     /locus_tag="LNE23_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBK6931737.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            658..1287
                     /gene="rplC"
                     /locus_tag="LNE23_001901"
     CDS             658..1287
                     /gene="rplC"
                     /locus_tag="LNE23_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBK6931738.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1315..1938
                     /gene="rplD"
                     /locus_tag="LNE23_001902"
     CDS             1315..1938
                     /gene="rplD"
                     /locus_tag="LNE23_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBK6931739.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1938..2228
                     /gene="rplW"
                     /locus_tag="LNE23_001903"
     CDS             1938..2228
                     /gene="rplW"
                     /locus_tag="LNE23_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBK6931740.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2267..3100
                     /gene="rplB"
                     /locus_tag="LNE23_001904"
     CDS             2267..3100
                     /gene="rplB"
                     /locus_tag="LNE23_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBK6931741.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3143..3421
                     /gene="rpsS"
                     /locus_tag="LNE23_001905"
     CDS             3143..3421
                     /gene="rpsS"
                     /locus_tag="LNE23_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBK6931742.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3444..3791
                     /gene="rplV"
                     /locus_tag="LNE23_001906"
     CDS             3444..3791
                     /gene="rplV"
                     /locus_tag="LNE23_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBK6931743.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3805..4461
                     /gene="rpsC"
                     /locus_tag="LNE23_001907"
     CDS             3805..4461
                     /gene="rpsC"
                     /locus_tag="LNE23_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBK6931744.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4464..4898
                     /gene="rplP"
                     /locus_tag="LNE23_001908"
     CDS             4464..4898
                     /gene="rplP"
                     /locus_tag="LNE23_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBK6931745.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4888..5076
                     /gene="rpmC"
                     /locus_tag="LNE23_001909"
     CDS             4888..5076
                     /gene="rpmC"
                     /locus_tag="LNE23_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBK6931746.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5101..5367
                     /gene="rpsQ"
                     /locus_tag="LNE23_001910"
     CDS             5101..5367
                     /gene="rpsQ"
                     /locus_tag="LNE23_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBK6931747.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5425..5793
                     /gene="rplN"
                     /locus_tag="LNE23_001911"
     CDS             5425..5793
                     /gene="rplN"
                     /locus_tag="LNE23_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBK6931748.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5830..6138
                     /gene="rplX"
                     /locus_tag="LNE23_001912"
     CDS             5830..6138
                     /gene="rplX"
                     /locus_tag="LNE23_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBK6931749.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6166..6705
                     /gene="rplE"
                     /locus_tag="LNE23_001913"
     CDS             6166..6705
                     /gene="rplE"
                     /locus_tag="LNE23_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBK6931750.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6724..6909
                     /locus_tag="LNE23_001914"
     CDS             6724..6909
                     /locus_tag="LNE23_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBK6931751.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6945..7343
                     /gene="rpsH"
                     /locus_tag="LNE23_001915"
     CDS             6945..7343
                     /gene="rpsH"
                     /locus_tag="LNE23_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBK6931752.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7375..7911
                     /gene="rplF"
                     /locus_tag="LNE23_001916"
     CDS             7375..7911
                     /gene="rplF"
                     /locus_tag="LNE23_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBK6931753.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8072..8428
                     /gene="rplR"
                     /locus_tag="LNE23_001917"
     CDS             8072..8428
                     /gene="rplR"
                     /locus_tag="LNE23_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBK6931754.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8449..8949
                     /gene="rpsE"
                     /locus_tag="LNE23_001918"
     CDS             8449..8949
                     /gene="rpsE"
                     /locus_tag="LNE23_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBK6931755.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8964..9143
                     /gene="rpmD"
                     /locus_tag="LNE23_001919"
     CDS             8964..9143
                     /gene="rpmD"
                     /locus_tag="LNE23_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBK6931756.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9187..9627
                     /gene="rplO"
                     /locus_tag="LNE23_001920"
     CDS             9187..9627
                     /gene="rplO"
                     /locus_tag="LNE23_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBK6931757.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9627..10922
                     /gene="secY"
                     /locus_tag="LNE23_001921"
     CDS             9627..10922
                     /gene="secY"
                     /locus_tag="LNE23_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBK6931758.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10984..11631
                     /locus_tag="LNE23_001922"
     CDS             10984..11631
                     /locus_tag="LNE23_001922"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBK6931759.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11822..12040
                     /gene="infA"
                     /locus_tag="LNE23_001923"
     CDS             11822..12040
                     /gene="infA"
                     /locus_tag="LNE23_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBK6931760.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12073..12189
                     /gene="rpmJ"
                     /locus_tag="LNE23_001924"
     CDS             12073..12189
                     /gene="rpmJ"
                     /locus_tag="LNE23_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBK6931761.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12207..12572
                     /gene="rpsM"
                     /locus_tag="LNE23_001925"
     CDS             12207..12572
                     /gene="rpsM"
                     /locus_tag="LNE23_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBK6931762.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12600..12989
                     /gene="rpsK"
                     /locus_tag="LNE23_001926"
     CDS             12600..12989
                     /gene="rpsK"
                     /locus_tag="LNE23_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBK6931763.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13069..14007
                     /locus_tag="LNE23_001927"
     CDS             13069..14007
                     /locus_tag="LNE23_001927"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBK6931764.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14035..14415
                     /gene="rplQ"
                     /locus_tag="LNE23_001928"
     CDS             14035..14415
                     /gene="rplQ"
                     /locus_tag="LNE23_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBK6931765.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14687..15526
                     /locus_tag="LNE23_001929"
     CDS             14687..15526
                     /locus_tag="LNE23_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6931766.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15502..16371
                     /locus_tag="LNE23_001930"
     CDS             15502..16371
                     /locus_tag="LNE23_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6931767.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16361..17158
                     /locus_tag="LNE23_001931"
     CDS             16361..17158
                     /locus_tag="LNE23_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBK6931768.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17361..18107
                     /gene="truA"
                     /locus_tag="LNE23_001932"
     CDS             17361..18107
                     /gene="truA"
                     /locus_tag="LNE23_001932"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK6931769.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            18772..19009
                     /locus_tag="LNE23_001933"
                     /pseudo
     CDS             18772..19009
                     /locus_tag="LNE23_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19153..19929)
                     /locus_tag="LNE23_001934"
     CDS             complement(19153..19929)
                     /locus_tag="LNE23_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6931770.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            20052..20459
                     /locus_tag="LNE23_001935"
     CDS             20052..20459
                     /locus_tag="LNE23_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBK6931771.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT         6976 a         3693 c         4414 g         5719 t
ORIGIN      
        1 tttattgcag gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa
       61 agagtcagta ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc
      121 cttgctttgt gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc
      181 acagacgtaa tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac
      241 gctttgccac gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata
      301 ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa
      361 caccgtattt tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct
      421 agcgtgtctg gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg
      481 actcataaat acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc
      541 gacattgtga acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct
      601 ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg
      661 accaaaggaa tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa
      721 ttaattccag taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg
      781 gaaacggatg gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca
      841 aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt
      901 gaattcaaaa atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt
      961 ttccaagcag gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg
     1021 gttatcaaac gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt
     1081 cgtcctgggt caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct
     1141 ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca
     1201 gaaagaaatg ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact
     1261 atcaaatcag ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg
     1321 aatgtagcat tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa
     1381 atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg
     1441 tcattaagac aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt
     1501 aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa
     1561 tggcgtggag gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct
     1621 aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac
     1681 ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt
     1741 cttgctaact tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt
     1801 gctgctttat cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt
     1861 gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt
     1921 gaggaggtgc ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa
     1981 aatcaatgct tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca
     2041 aaactttagt aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca
     2101 tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac
     2161 gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg
     2221 ctgaataagc actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa
     2281 gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac
     2341 aacatcaaca cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa
     2401 caacggccgc atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat
     2461 cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc
     2521 aaaccgttca gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt
     2581 agcacctaaa ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa
     2641 agtcggaaat gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga
     2701 aatgaaacct ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact
     2761 tggtaaagaa ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt
     2821 agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat
     2881 cggtaaagct ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat
     2941 gaaccctaat gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc
     3001 accagtttct ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa
     3061 atcagacaaa cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc
     3121 acattttgag aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga
     3181 gcatttaatg aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac
     3241 ttggtcacgc cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga
     3301 tggacgtaaa catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga
     3361 atttgcacct acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta
     3421 atttgagggg aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa
     3481 aaacagttcg cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa
     3541 gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg
     3601 aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa
     3661 acttggtagt aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc
     3721 gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat
     3781 cagaaaaata aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta
     3841 ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta
     3901 cacgaagatt taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca
     3961 acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca
     4021 ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa
     4081 ttaactggta aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca
     4141 aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct
     4201 caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta
     4261 tctggtcgtc taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt
     4321 cctcttcata ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac
     4381 ggaaaactag gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac
     4441 actgagaaag gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat
     4501 tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg
     4561 gtttgcaagc tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg
     4621 caatgactcg ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat
     4681 cttatactgc caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat
     4741 gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag
     4801 tagctcgtga agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa
     4861 aacgtgagga aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact
     4921 gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa
     4981 ttagcaacag gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca
     5041 cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt
     5101 atgactgaag gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg
     5161 gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc
     5221 atgaactatt ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac
     5281 atcgtgagaa ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa
     5341 gtagtcgaag aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca
     5401 aaatctgaaa ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat
     5461 aactcaggcg cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact
     5521 gctaacatcg gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc
     5581 aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca
     5641 gacggttcat atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc
     5701 cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg
     5761 aagatcgttt cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc
     5821 gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa
     5881 aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt
     5941 gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt
     6001 gagatggaag cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct
     6061 ggccgagttg gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt
     6121 gaagttttag ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa
     6181 gaaaaatatc ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt
     6241 atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca
     6301 aacgctaaaa acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca
     6361 atgattacaa aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt
     6421 ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct
     6481 gtttcactac cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt
     6541 ggaaactata ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta
     6601 gtagataaag tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa
     6661 tctcgtgagt tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac
     6721 tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa
     6781 gcttatactc gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt
     6841 tgccgtattt gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct
     6901 agctggtaaa aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc
     6961 caattgcaga ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag
     7021 aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg
     7081 ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat
     7141 ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc
     7201 gtgcttatgt caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca
     7261 tttcgacttc tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg
     7321 aagttatcgc ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc
     7381 cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac
     7441 aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa
     7501 atgaatatcg aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa
     7561 acaattcatg gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt
     7621 ttccaaaaag cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa
     7681 cttgtattga acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact
     7741 gttgaagtac cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc
     7801 gaattagctg caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt
     7861 cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga
     7921 aaaattgcat agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa
     7981 aatacatttt tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata
     8041 gtagtacaat aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac
     8101 acgtcaaaag agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg
     8161 cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg
     8221 tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga
     8281 agcagcacaa gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt
     8341 agtagtcttt gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc
     8401 tgctcgcgaa aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc
     8461 gatccaaaac atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt
     8521 gttaaaggtg gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga
     8581 cacgtagggt ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt
     8641 gaagatgcga agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa
     8701 gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg
     8761 gttgctgctg gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact
     8821 tctaaatctt taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg
     8881 aaacaattga aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta
     8941 atcggataag gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta
     9001 tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca
     9061 gttcagtggt taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc
     9121 tagtggacgt tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc
     9181 aaacctatga aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt
     9241 gttggtcgtg gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa
     9301 aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc
     9361 cgtcgtttac caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat
     9421 cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa
     9481 gctggaatcg tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac
     9541 aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg
     9601 gctggtggat caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa
     9661 aagacattag atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg
     9721 cacatattac tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat
     9781 ttttcaacat gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa
     9841 tgggggtttc accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg
     9901 tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag
     9961 cgactcgata tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat
    10021 tcaatcaata cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga
    10081 tcggtttgat tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag
    10141 aaaaaggtat cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc
    10201 ctgaaagtat cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat
    10261 ggaaatctgc tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg
    10321 taacattttt ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg
    10381 gcgcgcctac aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta
    10441 tctttgctag ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat
    10501 tcggttctga aaactggtac aaagtgatga tggagatctt taactataat acagcacctg
    10561 gtgctacgat ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag
    10621 taaaccctga gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac
    10681 gccctggtaa ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg
    10741 gtgcagtctt ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc
    10801 ttccacaatc aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag
    10861 aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt
    10921 aagaaagcgt gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa
    10981 acaatgaacc tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa
    11041 atcattgatg cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg
    11101 caaaacgaaa cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt
    11161 cctgatgaag taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa
    11221 ggcttcttat tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg
    11281 ctaaaagatt tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc
    11341 ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa
    11401 attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat
    11461 caaagagaag atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat
    11521 agtgaaccaa tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct
    11581 cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc
    11641 ccgtagccga cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata
    11701 tgtcagtccg acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga
    11761 actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg
    11821 cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc
    11881 aatgtttaaa gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat
    11941 tcgtatgcat tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata
    12001 cgatcttaac cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca
    12061 ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta
    12121 attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt
    12181 caaggataat tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg
    12241 ataaacgtgt agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa
    12301 agatcttagc tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac
    12361 aaacagatgc tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg
    12421 aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc
    12481 gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc
    12541 cagctcgcgc tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca
    12601 tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg
    12661 cacatatcca ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg
    12721 cattagcgtg gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt
    12781 ttgccgctca aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga
    12841 ctgttgaagt aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac
    12901 aagctacagg tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg
    12961 cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta
    13021 acgtcattga acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc
    13081 gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa
    13141 cctcttgaaa gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct
    13201 tctttgccag gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct
    13261 accatcaaag gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg
    13321 ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca
    13381 gtaactgctg gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac
    13441 atctgtagtg tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt
    13501 ggttatgttc aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc
    13561 gattcaatct acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga
    13621 caccgtgaag attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg
    13681 ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac
    13741 ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa
    13801 aaaatgttgg aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg
    13861 aaacgtgcag gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc
    13921 aaagtccgta acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta
    13981 ggtttaggtt tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt
    14041 tatcgtaaac taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact
    14101 gatttattaa tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca
    14161 actgcagaaa aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct
    14221 gcggctttcg tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt
    14281 gaatcagctt tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc
    14341 ggatacacac gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc
    14401 ttagaacttg tctaaaatca cactaataac atcactttat agatgattct tttagagtgt
    14461 tatgatgttg gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg
    14521 cttggggagt aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac
    14581 agatatgttt gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt
    14641 tttttgatag tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat
    14701 tgaattagaa aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga
    14761 tgtctccttt acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg
    14821 aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa
    14881 agtgggaaat caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat
    14941 ggtttttcaa aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt
    15001 tggtcttgaa aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact
    15061 tgagaaagtt agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca
    15121 aaaacaacgt gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga
    15181 tgaagcaacc agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa
    15241 gatcaaagaa gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc
    15301 taatgccaat cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa
    15361 agaaattttt tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga
    15421 aaaactgaag agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga
    15481 agaaaggatg gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag
    15541 ccgaatacac catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac
    15601 agttatactg cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg
    15661 aatgcgcttg ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg
    15721 gatacggata ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt
    15781 ccagaagcac agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac
    15841 tttggtgtca gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg
    15901 ctggatgaaa gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga
    15961 gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg
    16021 gcaggacttg atccccaggg tagaaaagaa atgatggaga tgttttggcg tcttcataag
    16081 gaacagcaga tcactattgt attagttacg catttaatgg atgatgtagc caattttgca
    16141 gattatgtct atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc
    16201 tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt
    16261 gcagaagaat taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa
    16321 ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt
    16381 tggtcgttat atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt
    16441 agccagtttt tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt
    16501 gttggcattt ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat
    16561 ccgaggcatt cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt
    16621 tacaagtggc ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt
    16681 gatgaacggc ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact
    16741 cacgcttact acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt
    16801 gaaagccgta cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt
    16861 tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt
    16921 tgattttggt gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc
    16981 tttatttgtc agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg
    17041 ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac
    17101 gatggttatc attgcttttg tattattaac tgtgctactg atctttttga gaggataaac
    17161 gttcagtggg ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcgagc
    17221 agtgtatgac aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt
    17281 atctgaaaag aaagaacgaa aaaattttat aaaaaaatca ttattccaga taacctttta
    17341 ggaacaaagg agagaaaaac atggtacgtt acaaagcaat cattgcatac gacggaacaa
    17401 attttaatgg attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa gtagaaaaaa
    17461 cattacaaaa aatggcaaat ggaaaagaaa tcacggtctt tggttcggga aggacagatg
    17521 ccggagtaca tgcgattggg caagtaatcc attttgatta tccagaggag cgtccgttag
    17581 aacgaatgcg tttcgcgcta gatacccagt caccagagga tattgctgta agacaagtag
    17641 aaattgtttc agaagacttc cacgcaagat atttggtcaa agaaaagacg tatcaatttc
    17701 gagtggatat agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt tattttcctt
    17761 accctcttga tttggaaaaa attcagcggg cgcttcctga tttattaggg actcacgatt
    17821 tcacctcttt ttgtgcatca ggaagctcga tagaggataa agtacgtacg atctacgagg
    17881 ctaagatgga ggtaaatgaa acaaaagacg aattgttgtt tacatttagg ggaaatggct
    17941 tcttgtataa gatgatacgg atcttagtag gaacgctttt gaaaattggc aatggccgat
    18001 tgcctgaaga tagtatccca gatattattg cgaaaaaaga tcgtaatgca gcaggaccta
    18061 cagcacatcc tgaaggactt tatttgtatg aagtgaaata tgaataagag gttgtgacag
    18121 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttacttctca aaattttgca
    18181 aaatttcggc ttattttcga aggagctact tctggaacac cgtgaatcag aggttgtgaa
    18241 aaagctgccc tgcatcaagt aataagaaca accaaacaaa aatagctcgt catatttttc
    18301 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg
    18361 tgaaatcagc gttttgacct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt
    18421 ttgccgtttg ttgagctaat accgaaggtc agctgattga acaccgttta agaaagaggt
    18481 cgtgagcctg tcatttaact ctgagtcaca agtaacaatt tcaaaaaaca gcttctcatc
    18541 tttgatgaat atcgtgactt gtggacgagg cgttgataag tgaatcatct ttttgttacc
    18601 tattttttct gtaaccaaaa aacgatggaa cgttgttttg attgccctta atgaggacag
    18661 tcggaacagg tttgtcattt ttatatttaa atggcacaaa tttttctaat tatttgtcat
    18721 ttagcgctga aaatttttgc tatttatcta aaaaagtgat aactacttaa gatgaagcag
    18781 aatatttacg ccatacggca tacaaccgcg aaaaatataa aaaaaagaaa acgattgaac
    18841 gcgtttttgc agtcgcaaag gaaaagcatg gaatgcgcta catcaagtat cgagacttgg
    18901 aaaacaatca ggtgtatacg ctgattgttt ttgctgcaat gaatctgaaa aagttgtcca
    18961 cttggcagtt ggaacatatg tcttcatatc ttttattata caaaaatagc aaaagcacca
    19021 gtctacactc ttagtaaaga ggaaactgat ccttttgtct acattcttaa agtacttaaa
    19081 ctaagtactt tattttactc tttaggattg atacagtagc atctatccct ttattaataa
    19141 tcttttattt ttttaaataa agatgttgtc tccatcttca ggcactaaaa ctttttcgga
    19201 aattcctttt tctttattga aagactttaa ttcttctctt gacagtcccc aatggtttac
    19261 agcttccata tgaacggcaa taattgtgga atctggtatt gctttatgca attcatataa
    19321 atcgtctttc cccataataa gagattctcc aactaaaaaa tgattatctc cagcatttgc
    19381 tactatgatt tctggattat actgatcaat agtttgttta acagcttcat accaaactgt
    19441 atctcctgta aagtatagtg tcttctcagt cgaatgtttg aaaacaatac cgcaaacatt
    19501 acctacataa ttcaagattt ctcctctgcc atgtttgcct tttgttttaa ccaattgtat
    19561 agattcaaaa actgtgtctt ctcgtagtac ctccacattt ttaaaaccat atagtcgaat
    19621 ttcattcgca tcattttcat cttgcacaaa taattttatt tctttaggta ggacctcttt
    19681 cgctatatcg tcccaatgat ctagatgtaa atgtgttaca ataacagcat caataccgtt
    19741 gataatatct tcgactggca ttggcaaatc aactagagga ttcatttgat cttttcttgg
    19801 tgatggaaaa ggacctttcg aaataaatgg tggaagtgtt cctttttccg ctaacattgg
    19861 atcaattaaa aattttttac ctccatagtc taagatagct gttgcattac gaatgtgatg
    19921 tatattcata aacgttgtct cctttttgtt gatatgaaat atttttagta taattcaatt
    19981 ccatacaata aataaaccga acaaaaaaag tcttttacta tttttactta atttatgaaa
    20041 ggttgtttac catgttagac caaacagata taaatatcat tgaggaacta tcaaaaaata
    20101 gtcgaatcac aatgaaagaa cttggagaaa aagtacattt gactggaccc gctgtctcgg
    20161 cgagagtgac aaaactagag gaaagtggag ttattgaaaa ttatacagta aaattaaata
    20221 tggaaaagct agggttttcg attcacgctt ttttaattat aattacgcaa aatttaaatc
    20281 ataaacccta tttatcattt attaaaaatc atgaaaaatt cttgattaga aattataaga
    20341 ttagtggagg gggctgttat cttttggaat gtagatttac ttcaaaaaaa tcaatgaaca
    20401 gctttttgga acagcttaat gaatatgcga attataaatt atctattgtt atagattaat
    20461 aataaaacta gcagatggta aaaatactaa tatgcgagac acacattcaa ccataactat
    20521 ataacttatc aatttaaaac taaactgatg gaaacaagta aggcaaaaac atttatattt
    20581 tgccctactt atttttttaa ttaaattcat tgatgattca tatttagaaa attctatgaa
    20641 ggccaaatga gtatcctgtc acttttttag ataaatagca aaaattttca gcgttaaatg
    20701 acaaataatt agaaatattt gtgccattta aatataaaaa tgacaccaag tatatcatga
    20761 acgcacaaaa agaccaacag ggtataccct gttggtctaa at
//
LOCUS       DAFCNS010000054        19609 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFCNS010000054 DAFCNS010000000
VERSION     DAFCNS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.055"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..3654
                     /locus_tag="LNE23_001936"
     CDS             127..3654
                     /locus_tag="LNE23_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBK6931772.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="LNE23_001937"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="LNE23_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBK6931773.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="LNE23_001938"
     CDS             7442..7783
                     /locus_tag="LNE23_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6931774.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="LNE23_001939"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="LNE23_001939"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBK6931775.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="LNE23_001940"
     CDS             9140..11560
                     /locus_tag="LNE23_001940"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBK6931776.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="LNE23_001941"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="LNE23_001941"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBK6931777.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="LNE23_001942"
     CDS             12994..13731
                     /locus_tag="LNE23_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6931778.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="LNE23_001943"
     CDS             13745..14560
                     /locus_tag="LNE23_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK6931779.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="LNE23_001944"
     CDS             14573..15220
                     /locus_tag="LNE23_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6931780.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="LNE23_001945"
     CDS             15236..15892
                     /locus_tag="LNE23_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6931781.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="LNE23_001946"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="LNE23_001946"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBK6931782.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="LNE23_001947"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="LNE23_001947"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBK6931783.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="LNE23_001948"
     CDS             18234..18752
                     /locus_tag="LNE23_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK6931784.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="LNE23_001949"
     CDS             18859..19359
                     /locus_tag="LNE23_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6931785.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6581 a         3369 c         4207 g         5452 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt agcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttt
//
LOCUS       DAFCNS010000055        19507 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFCNS010000055 DAFCNS010000000
VERSION     DAFCNS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.056"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="LNE23_001950"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="LNE23_001950"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBK6931786.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="LNE23_001951"
     CDS             complement(1173..1868)
                     /locus_tag="LNE23_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBK6931787.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="LNE23_001952"
     CDS             complement(1881..2597)
                     /locus_tag="LNE23_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBK6931788.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="LNE23_001953"
     CDS             2927..3532
                     /locus_tag="LNE23_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBK6931789.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="LNE23_001954"
     CDS             3857..4180
                     /locus_tag="LNE23_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBK6931790.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="LNE23_001955"
     CDS             4362..5732
                     /locus_tag="LNE23_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBK6931791.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="LNE23_001956"
     CDS             complement(5862..7163)
                     /locus_tag="LNE23_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK6931792.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9351)
                     /locus_tag="LNE23_001957"
     CDS             complement(7393..9351)
                     /locus_tag="LNE23_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBK6931793.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="LNE23_001958"
     CDS             complement(9528..9794)
                     /locus_tag="LNE23_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6931794.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="LNE23_001959"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="LNE23_001959"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBK6931795.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="LNE23_001960"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="LNE23_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBK6931796.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /locus_tag="LNE23_001961"
     CDS             13138..13701
                     /locus_tag="LNE23_001961"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK6931797.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="LNE23_001962"
     CDS             complement(13954..14475)
                     /locus_tag="LNE23_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBK6931798.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="LNE23_001963"
     CDS             complement(14478..15320)
                     /locus_tag="LNE23_001963"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBK6931799.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="LNE23_001964"
     CDS             complement(15313..16038)
                     /locus_tag="LNE23_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBK6931800.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="LNE23_001965"
     CDS             16214..16510
                     /locus_tag="LNE23_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931801.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17078..18670
                     /locus_tag="LNE23_001966"
     CDS             17078..18670
                     /locus_tag="LNE23_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK6931802.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="LNE23_001967"
     CDS             18683..19423
                     /locus_tag="LNE23_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6931803.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4002 c         3702 g         6223 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DAFCNS010000056        17166 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFCNS010000056 DAFCNS010000000
VERSION     DAFCNS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.057"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(38..847)
                     /locus_tag="LNE23_001968"
     CDS             complement(38..847)
                     /locus_tag="LNE23_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6931804.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1140..2576
                     /locus_tag="LNE23_001969"
     CDS             1140..2576
                     /locus_tag="LNE23_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK6931805.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2746..3942
                     /locus_tag="LNE23_001970"
     CDS             2746..3942
                     /locus_tag="LNE23_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK6931806.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4004..4189)
                     /locus_tag="LNE23_001971"
     CDS             complement(4004..4189)
                     /locus_tag="LNE23_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBK6931807.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4420..7734
                     /gene="dnaE"
                     /locus_tag="LNE23_001972"
     CDS             4420..7734
                     /gene="dnaE"
                     /locus_tag="LNE23_001972"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBK6931808.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLGPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            7902..8864
                     /gene="pfkA"
                     /locus_tag="LNE23_001973"
     CDS             7902..8864
                     /gene="pfkA"
                     /locus_tag="LNE23_001973"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBK6931809.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8938..10722
                     /gene="pyk"
                     /locus_tag="LNE23_001974"
     CDS             8938..10722
                     /gene="pyk"
                     /locus_tag="LNE23_001974"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBK6931810.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10757..11659)
                     /locus_tag="LNE23_001975"
     CDS             complement(10757..11659)
                     /locus_tag="LNE23_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6931811.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11845..12414
                     /locus_tag="LNE23_001976"
     CDS             11845..12414
                     /locus_tag="LNE23_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBK6931812.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12472..12651
                     /gene="rpmF"
                     /locus_tag="LNE23_001977"
     CDS             12472..12651
                     /gene="rpmF"
                     /locus_tag="LNE23_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK6931813.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12821..14242
                     /gene="gndA"
                     /locus_tag="LNE23_001978"
     CDS             12821..14242
                     /gene="gndA"
                     /locus_tag="LNE23_001978"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBK6931814.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14435..15121
                     /locus_tag="LNE23_001979"
     CDS             14435..15121
                     /locus_tag="LNE23_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6931815.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15118..16623
                     /locus_tag="LNE23_001980"
     CDS             15118..16623
                     /locus_tag="LNE23_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6931816.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         5550 a         2983 c         3596 g         5037 t
ORIGIN      
        1 ttgtcacaac ctcttttttg tggaatctaa ttctctatta tagtttaagt agtttttcca
       61 gataaacagc taaaccgtct tcctgattgc tcaaaggagt gacatcattt gcaatgcctt
      121 tgagttggtc agtaccgtta gccatagcta ctccccaacc tgcataatcc aacaattcca
      181 aatcattatg ctcgtcacca aaggcaatga cgtctttttg atcgatagac aggtagttag
      241 caatctcttg cacgcctttt gctttttgga ttccttttga aacaatctct aagatggcag
      301 taggtccacc ccatgtccga acatcgacat aactgccaaa ttgacgtgtt aattcagcag
      361 atacagctcc tgcaaaacgt ttgtctgttc ggacaagcaa agatgtagga tttgtcgtca
      421 aatttttagg agaaagcaaa ttcttctctc ctggacgaga agaagcaaaa attttttcat
      481 caaagaaatc aaaactgtca ataaaaaaag tatcacgatt ttcagcagcg ataaaatcta
      541 aatttagctg ctttttttgc gataagattt caaaagcgat ttcacgattg atcaacgttt
      601 ctctttcacc atcccagtgc tgattaggga tgtgtactaa tgcgccgtta aaattaacca
      661 tcggtgttgt taattctaat tgttggtaaa actgatggct cattcggaat gggcgtcctg
      721 tagcaatact gacatgatga ccggcattga ttgctttttt tagagtctct tttgttttag
      781 atgtaatcat cgaatcttga ttgagggtag ttccgtccag atcgatggca attaattttt
      841 tattcaataa gggtccttcc ttcctcaact agcatttgtt ttagtctagc atattttagg
      901 taattaacaa gagcaatcag ctattcctaa caaaaactac agttatggct atataaatag
      961 ctaaacataa gctaaacacg aacgttaagt attattttcg ctttttttgt taaaaaaacg
     1021 ttgaaaagcg aatgaaacgt ctatataatt tgaaattgtt cgatggattt gccctatttt
     1081 acttattttt tattattata gttttccatc agaaaaaaat tcagggagtg gaacttttta
     1141 tggaaaagtt tttcggttta aagaaaaaca atacaaatgt atcaactgaa ataatggcgg
     1201 gagtaacgac attctttgcc atgagttata tcttattcgt aaatccgacg attttgtcag
     1261 catcaggtat gcccttccaa gcagtatttc tagcgacaat cattgcatcg attattggta
     1321 cgctggtaat gggattgttc gcaaatgtac catatgctca ggcacctggt atgggattga
     1381 acgcattttt cacttttacg gtcgtttttg ggcttggtta ttcttggcaa caagccttgg
     1441 caatggtgtt tatttgcgga ttaatcaata tttttattac tgtcacaaat attcgaaaaa
     1501 tgattattcg ggcgattccc gaaagtcttc aacatgcgat tggtggcggg atcggtattt
     1561 tcgttgcgta tgtcggaatc aaaaatgcag gattcttatc tttttcagca gaccagtcag
     1621 ctatttcgag ctctgtcgtt gaaggcggaa aagcgacaaa tgttacgata aatggtggaa
     1681 tcgtccctgc gttagccaac tttgacaacg ctcctatctt attggctgtg attggtttgg
     1741 tactgacagc cattttagtt gtaaaaaatg tccgtggggc tattcttatc ggtatcgtag
     1801 cgaccacagt ccttggtatt tttatgggtg ttgttgattt aagttccatt gattggcaaa
     1861 cgaattcgtt aggtaattca tttaaagaat taggaacaac tttcggtgca gcatttggtt
     1921 ctgaaggcat gcaatcttta ttcagtgatt cttccaagat tcctcaagta ttgatgacga
     1981 tcatcgcatt cagtttatct gatactttcg atacgatcgg aacatttatc ggtacaggac
     2041 gccgcacagg tattttctca aaagaagacg agatcgcgtt ggaagatggt cgtgggttca
     2101 aaacaaaaat ggataaagca ctatttgccg atgcgatcgc tacttctgtg ggggctatct
     2161 ttggtacatc gaatacaaca acttatgtcg aatctgcagc tgggatcggg gccggtggac
     2221 gtactggttt gacttcagta gtagtggcag tgctattcgc attaagtagt ttattctctc
     2281 cactgatcgc cattgtacca aatcaagcaa ctgcgccagc attgatttta gttggagtaa
     2341 tgatgatggc ttcttttgca gacatcaaat ggctagatat ggaagaagct ttaccggcct
     2401 tctttgcatc gatctttatg ggattgtgct atagcatttc ctatgggatt gcagcaggat
     2461 ttattttcta tacaattgtg aaagtaatca aaggaaaata caatcaagtt tctgttgctt
     2521 tgtggatcgt tgatatttta ttcattatca acttcatcat tctagcaaca atttaactaa
     2581 agagaaggga ttggaacagt atttgttctg atctcttttt gttttacata aaaagaacgt
     2641 tacatataaa atgacctttg cgggattttt ctctttttac tggcgagatc gttgcttgtt
     2701 tattcgaaaa taggtatgat aaaagagacg aaagagggat gatagatgga gattaattgg
     2761 agaaaaaatt tagtgattgc gtggattggc tgttttttca ctggtgcaag tattagttta
     2821 gttatgccat tcataccagt ttatgttgag caactaggaa caccaaaaga tcaaatcgaa
     2881 ttgttttcgg gactagcaat ctctgtgact gcttttgcat ctgccgtggt tgcaccaatc
     2941 tggggaaatc tagctgatcg aaaaggccga aaactcatga tgatccgtgc agcagcaggg
     3001 atgacattaa caatgggatc tttggctttt gtaccaaacg cgtattggct tttgatcatg
     3061 cggttttgga atgggatttt gtctggatat attccaaatg ccacagcgat gattgcttca
     3121 caggcaccta gagaaaagag cggatgggcg ttaggaacat tgtctaccgg agcgattgct
     3181 ggaaatctga tcggaccttc aatgggcggt gcactggctc agtggtttgg tatggaaaat
     3241 gtattcttga tcacaggggc tttgttgatg atcacaacgg tattgaccat tttcttagtc
     3301 aaagaagatt tccatccaat cgagaaaaaa gatctgataa gcaccaaaga aattttttca
     3361 aaaatggatc atctctctat tttgatcggt ctcttcatta cgaccttgat tttacagata
     3421 ggaatcacta cgatcagccc tattttgacc ttatatattc gtgaattgag cggaagtaca
     3481 gaaaatatcc tttttgtcag cggcttgatt gtttcgatag ctggtgtttc cgctgtcttt
     3541 tcttcaccga aactaggaaa actaggggat aagattggga atcaaaaagt tctaattgga
     3601 ggactcattt tatcattttg ctgttattta cctatggcat tcgtcactac accgctccaa
     3661 ttaggtatac ttcgctttat tttaggcttt tcaactggcg ctttgatgcc ttctgtcaat
     3721 acactgatca gtaagattac gcctcaagaa ggagtcagcc gaatctatag ctacaatcag
     3781 atgtttagca actttggaca agttttaggt cccatgctag gatcgacagt cgcgcatgct
     3841 tataattaca gtgctgtttt tattgtgaca agtttgtttg ttctgtcaaa tattcttctt
     3901 tctttattca actttcgtaa agttcttcgc agcagattat aatggtggtg aggagtagaa
     3961 aataaaaaag agcttgagaa atctcaagct cttggctgtt tttttacgaa tgggcagagg
     4021 tttcttttct tggcgcaact gcaccaatca aaaggataat gacacccgca atgaatgaaa
     4081 tgattgttgt caaagtaaaa tcatatgttc cgcttgttaa tgcagcacca atataaccga
     4141 ctacttgacc tagaacgaca gcccagaata atgtaacgat atatcgcata aagaacacct
     4201 cttttctatc ttaattttac cagattttct ttgaatgtaa agaaacgaga ccaaatctca
     4261 ataaaatttg agttcttgcg aaagaactca ttcctacaga ttacaagcaa gcaaatgaag
     4321 tggaataatc aacttcagcg cctgctatct taatgaaagt aaaaggatca aaccttttaa
     4381 cgctagtgaa ttttaacgaa aaaaaaggcg gaatgcaaga tgtttttccc acaactttat
     4441 acaattactt cttattctct tttacaaagt acgatacgta ttcgagaata tgtacaagaa
     4501 gcaaaaaaac aaggctacac ttcattagcc ataacggata aagatgtctt atacggtgca
     4561 cctgaattct atcgtatttg tgtagaagaa ggagtacagc caatcattgg attacatctt
     4621 agttatactt atgaagaaaa aaattatggc ttgcttgcgt acgcaaaaga tgaaataggt
     4681 tatcagcagc tgatgaaact ttccagccga aaaatgatcc aagaaaaatt ggaacttgca
     4741 gaaatgacag aaaccgacca tctagcgttt gttgttccgg caaatcattc tgtttctgag
     4801 gctttcaata aagaggataa ctttttcggt cattggcagt ttttacttga aaatgttttt
     4861 actagaaatt tttatatcgg cttgacagaa aacacgcctg atcaattgcc tgaatggtat
     4921 cagtatgtga taaaagaaaa ttattcgctt tgtgcattac atcccatcgc tagtttacaa
     4981 caagaagaac tttttgcact tgaagtgatg aaacatatca aagagggaag ccaaatacct
     5041 ttagaaaatt tatctgcatc tttaccacta acagcaaatg tctatcttct ttcagaacaa
     5101 gaaatgatca aacgattcga aaaagcgggt ctttcccaag ctgtaatagg cgcccagcgt
     5161 ttagctgaat cgatttcatt tgaattccag ttccatcaga aattacttcc gcattatcca
     5221 gttcctaatg gaaaacaagc tggagaatat ttaagagagt tatgctattc gcaagtggaa
     5281 cgccgaatca ctgactatac aaaggaatac caagaacgtt tggactacga gttatctgtc
     5341 atccatcaaa tgggattcga tgattatttc ttgatcgtat gggatgtaat ggcttttgct
     5401 catagaaaca aaatcgttac cggtgcaggt cgtggttctg ctgcgggttc attagtagcc
     5461 tatgtgttag aaatcacaga tgtggatcca ttagaatatg atttgctgtt cgaacgcttt
     5521 ttaaaccccg aacgctttac gatgccagat atcgatttgg atattcctga caatcgacgt
     5581 gaagaagtat tgaattatgt tcgagaaaag tatggacaat atcatatggc acaaatcgca
     5641 acatttggca caatggcagc aaaaatggtt cttagagatg cagctagagt gtttggttta
     5701 tcgcaaagtg aagcaaaccg ctggtcaaaa gcaataccca atcaattgaa aatcacattg
     5761 acccaagctt ataaaacttc gcatcaatta gtagaattag ttcaggcaag cgaaaaaaac
     5821 caacttttat tcaaaactgc agtgatttta gaaggattgc caagacatgt ttcgactcac
     5881 gcagcaggtg tagttatcag cgataagaat ctacttgaac tagttccgct ccaacgcggt
     5941 tctgatgaag cttttctgac ccagtatacg atgaatgagg tagaagcaat cggtcttttg
     6001 aaaatggact ttttaggact acgcaactta tcgattattg attttacttt gaaagctgtt
     6061 aagcgagtag aagggtatga gatccgatta aaagagatcc cgctaaatga tgcaaaaacg
     6121 ctgctcttgt ttcaacgagg cgaaacctca ggagtatttc aatttgaatc tgctggtatc
     6181 cggaacgttt tacgaagatt aggtccagaa aacatcgaag atattgccgc agtcaatgca
     6241 ttgtatcgac caggccctat gcaaaatata gataccttca ttgccaggaa gaaaggaaaa
     6301 gaagccattc gctacccaga tgattcactg atcccgattt tgaaaaacac atatggagtg
     6361 atcgtctatc aggaacaaat catgcagatc gcctcgacta tggctggatt tacattaggg
     6421 cagtcagata ttctgcgacg ggcagtgagt aaaaagaaaa aagacgtatt agatgaagaa
     6481 aagtcgcatt ttgtatcagg agcattacaa cagggatatt cggaaaaaac agcagcgacc
     6541 gtatatgaat atatcgaaaa atttgcaaac tatggattca atcggtcaca tgcatttgct
     6601 tattcattcg tgggattcca aatggcgtat ctaaaagccc atcatccagg tgctttcttt
     6661 accagcctga tgaattctgt tcggcataac acatcaaaat tgaaagaata tattgcagaa
     6721 gcacgtaaaa ataaactcaa actttcctcg ccgtccatta atcaaagtaa ttccagtttt
     6781 gagttggtca ataaggaaga aatcagattc ggactgacag cgataaaagg tattcggcgt
     6841 gattttgtag aagatatatt aaacgaaaga aaacaaaatg gcccgttcca gtcagtggat
     6901 caattcctca tacggatcga taagcgttgg ctgaaattag agttactgca atcactgaca
     6961 gcagtaggcg tttttgatga attatctcct aacaggaaac aattgatgct tgatctcgaa
     7021 ggaaaaatcc aaaatatcgt ttacagtggt ggaagtcttg acttgctggg aatcatggca
     7081 ttaaaagaag aagaagtcgc tgactataca ctagaagaac gcttgaagct ggaagaggag
     7141 tacttaggta tttatttgtc aggtcatcct gcagaagggt ttgatcgtct tcgtttagct
     7201 aaacaagttc agctgattac agatatcgtc cctaatcaac cagtcaattt attagtgatg
     7261 atcaaggaag ttagagaaat tcggacaaaa aaaggagaac tgatggcctt tgttgaagga
     7321 accgacagta gtggagaaat ttctgtcaca gtttttccaa ctctttatcg taaatatcgt
     7381 aatttgtttg ccgttgatcg agtgatcttt gttcaagggc ggagcgaaat cagcaaatat
     7441 aatcaggaac tgcaactaat tggagaaagc tttgctgatc ctaaccagtt agaagcccag
     7501 tatccttcac aaacttgcta tttgcgtatt acagaagaac gggaagatcc cgagataatg
     7561 aaacaattac aagtgatttt tcagaaacac ccaggatata taccagtagt tatgtatcac
     7621 gaaaaaacgc aacgaaaagt agttctgtca gatgcttatt gggtggatga cagcaagagt
     7681 ataaaggggc aacttattta tctgcttggc gaagataatg tggttttcaa ataacttacg
     7741 gtaaaaaata ctacttgctt tcattttttt tcattttttt atccgtaaat caagacaagt
     7801 gagaaaaata atgttagaat gttagtcgtg gttaacgcaa aaatggttgt aaacctaagt
     7861 catacttagg ataaagtatt ttaatgaggt gaatgtttat tatgaaacgc atcggaattt
     7921 tgaccagtgg cggagacgca cctggaatga atgcagctgt tcgtgccgtt gttcgtaaag
     7981 gtatttacga aggactggaa gtatacggga tcaattatgg ttttgccggt ttagttgctg
     8041 gagatattcg tcgtcttgat gtggcagatg taggtgacaa gatccaacgt ggtggtacat
     8101 tcttgtattc tgcacgttat ccagaatttg caactgaaga aggtcaatta aaaggaatcg
     8161 aacaattgaa aaaattcgga atcgaaggtc tagtggttat cggaggagac ggctcttatc
     8221 atggcgcaat ggctttgaca aaacgcggtt tcccagcagt aggtattcct ggaacaatcg
     8281 ataatgatat tcctgggact gatttcacaa tcggttttga tacagcaatc aatacagtac
     8341 tagaatcaat tgaccgtatt cgtgacacag cgacttctca tgttcgtaca ttcgttatcg
     8401 aagtaatggg acgcaatgca ggagacatcg ctttatggtc aggtgtggct ggcggagcgg
     8461 atgaaatcat catcccagaa catgattttg atatggcttc agttgctaaa aaaatccaag
     8521 aaggccgcga tcgcgggaag aaacattgct tgatcatctt ggctgaaggt gtaatgggcg
     8581 gaaatgaatt cgctgagaaa ctttcagaat ttggcgacta tcatacacgt gtatctatct
     8641 tgggacacgt tgtccgtggt ggagcaccaa gcgcacgcga ccgcgtgttg gcaagtaaat
     8701 tcggtgctta cgctgtcgaa ttgcttcaag aaggaaaagg tggattatgt gtcggtatcc
     8761 atgacaacga aatcgttgcg tctgacatca ttgatacact tgaaaacaac aaacataaac
     8821 cagacttatc actatatgat ctaaataatt cactttcatt ttaagagtga aagaaaccaa
     8881 gctatttaag ctgtgtacag ctttaaataa acaaaattga atgggagagt tttttaaatg
     8941 aaaaaaacca aaatcgttag tacattagga ccagcaagta actcagtaga aattatttct
     9001 caattgattg aatctggggc aaatgtcttc cgctttaact tttctcatgg tgaccatgaa
     9061 gaacaattat cacgtatgac tatggtccgt gaagcagtta aaaagacagg aaaagatgtc
     9121 ggtattcttt tagatacaaa aggagctgaa atccgtacaa ctgttcaagg cacaacagaa
     9181 gctgattttg gtcgtgcagg atacatcaaa tttgaagtag gagatcaaac acgcatctct
     9241 atggatcctg aacatgtagg ttcaaaagaa aaaatcgcgg ttacttaccc aggattattt
     9301 gacgatgttc acgttggcgg acacatttta tttgatgatg gtttgatcga tatgcaaatc
     9361 attgaaaaag acgaagcaaa ccgtgaatta gtcgtagaag ttaaaaatgc aggtatgcta
     9421 ggatcacgta aaggggtaaa tgcaccaggc gtttctatta gtttgccagg tattactcca
     9481 aaagatgctg acgatattcg ttttggttta gacaacgata tcgactttat cgctgcaagt
     9541 tttgtacgta aagcacaaga cgttctagat atccgcgaaa tcttagaaga aaaagacatg
     9601 actcatgttc aaatcttccc taaaatcgaa tctcaagaag gtatcgacaa cattgacgaa
     9661 atcatcaaag tttctgacgg tgttatgatt gcccgcggag atatgggtgt tgaaatccca
     9721 gctgaactag taccaatggt tcaaaaacgc atcatcaaaa aatgtaatgc tgctggtatc
     9781 ccagttatca ctgctacaca aatgttagaa tcaatgcaag aaaacccacg tccaacacgt
     9841 gcggaagctt ctgacgttgc taacgctgta tttgatggca cagatgcaac aatgctatct
     9901 ggtgaatctg caaacgggga ctacccagtt gaagctgttt caacaatggc acgtatcgac
     9961 caagaagctg aaaatgcatt atcagaatta ggtacattcc aattgaacga attcgataaa
    10021 acagatgtaa ctgaaacaat cggtttatct gtagctcgtg ctgcaaaaaa tctaggcgtg
    10081 aaaacaatcg ttgctgcaac tgaatcagga tacactgcaa aaatgatttc aaaatatcgt
    10141 ccagatgcag atatcttggc tgttactttt gacgaacgca caaaacgtgg tttgatgtta
    10201 aactggggcg tttacccaac agttgctgaa aaaccatcaa caactgatga aatgtttgaa
    10261 ctagcagcta aaaaagctgt tgaattaggt ttcgcatcag aaggcgattt aatcttgatc
    10321 actgctggtg taccagttgg tgaacgcgga acaactaacg tgatgaaaat tcaaatgatt
    10381 ggttctaaat tattagaagc tcaaggcgtt ggacaaaaat ctgtcgttgc aaatgcagtt
    10441 attgcttcat ctgctgaaga agctgtaaaa aaagcaaaag acggaatggt tctagttgta
    10501 ccatcaactg acaaagaata tatgcctgca atcgaaaaag cagctgctgt tatcgttgaa
    10561 gaaggcggat tgacttctca cgctgcagtt gtaggaattg cacaagatat tcctgtaatc
    10621 gttggtgcta aaggcgcaac aacaacaatt acagaaggtg aattgattac cgttgattca
    10681 cgtcgcggca tcgtttaccg tggcgaaaca acagctatct aatttagctt aataaaaaat
    10741 aaaaacagct cttcttttac gaagagctgt ttttttgtgc tgtaacgaat tcatggataa
    10801 cttggactac ttgttcttga agaggcgtga cgatctgctg tgttcgtttt gccaatgaaa
    10861 tcaagaagcg aggctgaact tcatctgaaa gagggaggca aactaaatga tcttcaggat
    10921 ggacagcaat ctctgccaaa aaaccgatac ccacattttc tcggaccata ctctttaaaa
    10981 tcgtcaagtc attgctttga taaacaactt ttggatgagt atgtgtttgt ttagaaagct
    11041 tagtgaatgc tgcggggtgg acataatgtt cgttcaatag tacaaactgt tcatctgcta
    11101 tatctttgaa agagacagta tcctgcttgg aaagaggatg tgtaggtggc acgacgatca
    11161 tgaaacgttt gtccaaaagt aactctgttt ctaaatattg atccacgatt ggctgtgttg
    11221 agcctaatac agctaaatcg atcctccctt gtcggatcat cccgtataag tcccgagagc
    11281 ctccatcggt aatctcaatt tgattcatca attcatgctt gataaagtaa cttgacagtt
    11341 taggaaagta ttcattgccg ataataggag gcatgccaaa acgaagcttt ttcttttgca
    11401 actgagtgat ttcagctctt gcagtagaaa tctcttggag aatggtttgt gcatggagaa
    11461 acagctgctt tccagaaggc gtgacgatta attcgtgatg cgatcgatct cttgtaataa
    11521 gtacggtgtc aaactctttt tcaagacgtt gtaatgcata tgtaatagta ggttggctca
    11581 cacggaaata attagccact ttcgtaaaat ttttttgttt gacgatttgg acatagtatt
    11641 cgatatcttt cgtgttcatg aagatcatcc gctcctttaa tggaataatg attattgtaa
    11701 atgattctga ggatgatagc aaacagaggc tttctaataa agatttttct gtttttttat
    11761 gaactgcaag gtgtcaagaa aatttcattg acaacatgct ttttgccctg tataattgct
    11821 tttgttgctt aggagtgatg aaaaatgaaa tggtctttat tggaattgag aaaataccag
    11881 gagacaccgt taacttttca tgagacgctc gacttgaaag ccgctctcat gaagcgagac
    11941 aatttaatac ttgatgttgc gccagtggaa gtcgaaggtt tggtctcagt tgataagaaa
    12001 ggctatatcg ttcattacac tgtgcagacg actttgacag ttccttcaac acgttcacta
    12061 gaacctgttg aactgcccat ggatttttct gtagacgaag tattcatgac agaagaacaa
    12121 ttccaaagta gagatgaaca aatgtctgcg gatgagattt tgctaattga aggtccatta
    12181 ttgaatttag cagagtcgat tgaagacaac atcttattgg cgattccaat gcgggtattg
    12241 acaaaagaag aggaagcagc aacagaactt ccaaaaggca atgattggga agttgtttcc
    12301 gaagaagaat accagcgcag aaaaatggaa gaagccgaga ataaaattga tcctcgtctt
    12361 gcaaaactat ctgaactttt tgattctaaa tcaaaagacg acgataacaa gtaagattgg
    12421 aatatattgt gtctaattta gatacaagac tttacaagga ggtgtagcaa aatggcagta
    12481 ccagctagaa gaacatcaaa agctaaaaaa gcaaaacgtc gtacacacta caaattgaca
    12541 atcaaagggt taaacgaatg tccaaactgt ggtgaaatga aaaaaagcca tcacgtatgt
    12601 gcaaattgtg gttactatga tggtaaggac gtaatgtcaa aagaagctta atacatttta
    12661 attagaaaat gtaaaacctc aaggctaacc atggagcctt gaggtttatt tttttgtagt
    12721 cggtaaataa aaaatatgtt gttctttagt ttgcaatgta gccgtgttaa gctataatgt
    12781 aagagattag ggagaaatcc cgtatgtagg aggagttcat atgtcaaaac aacaaatcgg
    12841 cgttgtcggt atggccgtta tgggtaaaaa tctagccttg aacatagaaa gccgcggcta
    12901 ttcagtggct ttattcaatc gtacaggggc taaaacaacc gctgtagtag aagaacaccc
    12961 agataaaaat ttcaaagcaa catatacaat cgaagaattt gtagaatcta tcgagaaacc
    13021 acgtcggatc ttgctaatgg tcaaagcagg accggcaaca gatgcaacga tccaagagtt
    13081 gcttcctcac cttgataaag gcgatatttt gattgacgga ggaaatacat tctttaaaga
    13141 tacgatgcgt cgtaatgaag agttagccaa ctctggcatc aactttatcg gtacaggcgt
    13201 ttccggtggt gaagaaggag ctttaaaagg accttcaatc atgcctggtg gacaaaaaga
    13261 agcctatgcg ctagttgcac caatcttaga gcaaatttct gcaaaagcag aagacgaagc
    13321 accatgtgtc acatatattg gcccaaatgg agccggacat tatgtgaaaa tggtacacaa
    13381 cggtattgag tatggagata tgcaactgat tgctgaatct tatgacttaa tgaagaatat
    13441 tttaaacctt tcagtagaag aaatggctga tatctttaaa gaatggaatc aaggcgaatt
    13501 agacagctat ttgatagaaa tcactgctga cattctgaca cgtaaagacg atgaaggaac
    13561 aggtaaacca gtagtagatg tgatccttga tgcagctggg aataaaggaa caggtaaatg
    13621 gacaagccaa agcgcgcttg acttaggtgt gccacttcca ttgatcactg aatctgtatt
    13681 tgcccgctat atttctgctt ataaagaaga acgcgtacaa gcaagcaaga ttttatctcg
    13741 tacgaacgat tttgagttca cgggagacaa aaaagaattg gtcgaaaaaa tccgagaagc
    13801 gttgtatttc agtaaaatca tgagttatgc ccaaggattt gctcaattac gtgtcgcttc
    13861 aaaagaattt gattgggact tgccatttgg tgaaatcgca aaaatctggc gcgctggatg
    13921 tatcatccga gctcgattct tgcaaaaaat tacggatgcc tatgacaaaa atcctgagat
    13981 cgaaaattta ttgctagatg attacttcgt tgaaatcacg aaaaaatatc agcaatcagt
    14041 cagagacgtc gttgccttgg ctgtccaagc tggcgtacct gtaccgactt tctcatcagc
    14101 catcgcttac ttcgattcat acagagcaga acgtctgcct gcaaatataa tccaagccca
    14161 acgcgattac tttggcgcac atacctatga acgtgtcgac aaagaaggaa tcttccatta
    14221 ctcatggtac catgaagaat aatagtcttt cggctgttag gaaatgagaa aataaaaaga
    14281 ggacgaaaga gctgaagtcg attgttatga gtagacttca gctctttttt aaaattgcta
    14341 tccatgataa aatgactata gatttttaag catattatcg gtataatgat agagttttta
    14401 aaaagccagt agaatagaaa ggataaaata ataaatgagc aatattctaa tcattgaaga
    14461 tgaaaagaac ttggcacgat ttgtcgagct agagttgaag catgaagggt ataacgcaga
    14521 agtccactac aacggccgaa ctggtttaga tgcggcatta aataatgagt gggatgccat
    14581 attgttggat ctgatgctcc cagaattgaa cggtttagaa gtctgcagac gtgtacgcca
    14641 agtcaaaaat acaccaatca ttatgatgac tgcaagagat tctgtgatcg atcgtgtatc
    14701 tggacttgac cacggagcag atgactacat cgtcaaacca tttgctatcg aagaattatt
    14761 agcacgtctt cgtgcattgt tgcgtcgaat cgatattgaa ggcgataaaa atgtggctaa
    14821 acaaactact attacttatc gcgatttgac tatcgaaaaa gaaaaccgtg tcgtacgtcg
    14881 cggaaatgaa gtaattgaac taacaaaaag agaatacgag ttgctgctta ctttaatgga
    14941 aaacgtgaac gtggtattag cacgtgacgt tttactaaat aaagtgtggg gatacgaaac
    15001 agaagttgaa acaaacgtag tggacgtata tattcgttat cttcgcaaca aaattgacgt
    15061 tcccggggag gaaagctata tccaaactgt acgcggaaca ggttatgtga tgcgctcgtg
    15121 ataaagccga aaaaatcgga aaaaagagaa ctgaaagggc cctccttgac aatcaagtgg
    15181 gcctttgcga gttctttctt catattcgtt gtgtttacaa ttttcgctgt cattacctat
    15241 aaatcatcta tcaatctgat tgtggcaaaa gaaagaaaca atgtagagcg tacgatttca
    15301 gaagtagctt ctcgtttggc taactcggat gaagaactga cattagtaaa tacgtatcgt
    15361 aatctgacga aagcgacaaa tgacggagat gaggtcgtag atcatacttc actggtcgaa
    15421 ggaaacttaa tgaagatcga ttcctttata gctgagctag ggcagccatc aatggatctt
    15481 tatgtttatg atttggacga gaacttgatt tttaaaaccc atgaaaacag tcgaaacctg
    15541 attcagacta cacgtaaagt tccaacgatc gttacactag aaggaaaaac tggcttttta
    15601 tctatccagc ctatatattc taaaggtaca agagaaaaga tcggctatgc gcaaagcttc
    15661 tatgaattat cttctttcta tgatattcga aataagctat tattgactct tattgtttta
    15721 gaagttgttt ctttgatctt aagtagtatt ttaggtttct ttttatcttc ttatttctta
    15781 aaaccactca aggtactcag agatacaatg gatactatac gtaaagatcc gcaatcagat
    15841 atccacatgc ctgaaatcga cacaaatgat gaattggcag atttagctga aatattcaat
    15901 gagatgcttg accgtatgcg tttgtatatt gaacaacaag agcaattcgt ggaagacgta
    15961 tctcatgagt taaggacacc tgttgcaatt attgaagggc atcttagtct gctaaatcgt
    16021 tgggggaaag atgatcctga gatattagaa gaatcattaa gcgccagcat gcaagaaatt
    16081 agccgtatga aaagcttagt tcaagagatg ctagacttat caagagcaga acaagtagat
    16141 gttcattatg cgaatgaaac aactaaagga aaagaagtca cctatcaagt ttataataat
    16201 ttcaagatat tatatccaga ctatgtgatc actttagatg atgatttgac aaaagaagtg
    16261 accctgcaaa tttatcgtaa tcactttgaa caattaatca ttatcatatt ggataacgca
    16321 gtaaagtatt caactacacg aaaagaagtc catatctcta tttcttccac tctgaatgaa
    16381 tttgagatcg cgattcagga ctttggcgaa ggaataccca aagaagacct taataagatc
    16441 ttcaatcgtt tttatcgagt ggataaagca agagctagaa cgaagggtgg aaatggtctg
    16501 gggttgtcta tcgctaaaca actggttgaa aactataaag gacgtatctt agcagaaagt
    16561 gtagtagggc aagggacaat ttttagaatc tttattccta tcgcaaaggc agaagacaag
    16621 taaagcatgt gctttacttg tttttttata tatccaacaa agaataaggt acaaaataca
    16681 agcaagttag aaattcaggg atgtcattat aaaaaaaact agcgaacaat taaatagaat
    16741 aacatccgtt aattaaacgt aagtagcttt tagatgaacg aaaaaacaat gaaatgctgt
    16801 aatattcgtg tttaaaaata ataaataaaa atagggaatt ttctattgca attttataaa
    16861 gagattgcta tattattatt tgttctaaat gattccttta aggtgtcact aaaaattata
    16921 aaaaataggg ttgacgcact tttgatgtca tgttataata acgaagtcgt ttggatgagg
    16981 tagctaataa agcttgattt tatcgaaaaa aacttcgaaa aagttgttga caaataacaa
    17041 taaagatgat atgataatca agtcgctgct aatgagtgac aaagacgttt agaaaaaata
    17101 aacaaagaag ttgttgacat tctcttctga atctgataag attaaagagt tgttgaaaca
    17161 aaaaga
//
LOCUS       DAFCNS010000057        17006 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFCNS010000057 DAFCNS010000000
VERSION     DAFCNS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.058"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..127)
                     /locus_tag="LNE23_001981"
     CDS             complement(<1..127)
                     /locus_tag="LNE23_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBK6931817.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(231..542)
                     /locus_tag="LNE23_001982"
     CDS             complement(231..542)
                     /locus_tag="LNE23_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6931818.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(544..741)
                     /locus_tag="LNE23_001983"
     CDS             complement(544..741)
                     /locus_tag="LNE23_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBK6931819.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1135..2862)
                     /locus_tag="LNE23_001984"
     CDS             complement(1135..2862)
                     /locus_tag="LNE23_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBK6931820.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2855..4048)
                     /locus_tag="LNE23_001985"
     CDS             complement(2855..4048)
                     /locus_tag="LNE23_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6931821.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4235..4879)
                     /locus_tag="LNE23_001986"
     CDS             complement(4235..4879)
                     /locus_tag="LNE23_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6931822.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4973..5107)
                     /locus_tag="LNE23_001987"
     CDS             complement(4973..5107)
                     /locus_tag="LNE23_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBK6931823.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="LNE23_001988"
     CDS             complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="LNE23_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBK6931824.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7238..7711)
                     /locus_tag="LNE23_001989"
     CDS             complement(7238..7711)
                     /locus_tag="LNE23_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBK6931825.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8112..8336
                     /gene="nrdH"
                     /locus_tag="LNE23_001990"
     CDS             8112..8336
                     /gene="nrdH"
                     /locus_tag="LNE23_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBK6931826.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8495..10654
                     /gene="nrdE"
                     /locus_tag="LNE23_001991"
     CDS             8495..10654
                     /gene="nrdE"
                     /locus_tag="LNE23_001991"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBK6931827.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10704..11669
                     /gene="nrdF"
                     /locus_tag="LNE23_001992"
     CDS             10704..11669
                     /gene="nrdF"
                     /locus_tag="LNE23_001992"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBK6931828.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11758..12897)
                     /locus_tag="LNE23_001993"
     CDS             complement(11758..12897)
                     /locus_tag="LNE23_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6931829.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13206..13919)
                     /locus_tag="LNE23_001994"
     CDS             complement(13206..13919)
                     /locus_tag="LNE23_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBK6931830.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="LNE23_001995"
     CDS             complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="LNE23_001995"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBK6931831.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15108..16640
                     /locus_tag="LNE23_001996"
     CDS             15108..16640
                     /locus_tag="LNE23_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6931832.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16831..>17006)
                     /locus_tag="LNE23_001997"
     CDS             complement(16831..>17006)
                     /locus_tag="LNE23_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6931833.1"
                     /translation="FMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQT
                     VVFKGKRNLENKK"
BASE COUNT         5277 a         3493 c         2911 g         5325 t
ORIGIN      
        1 atgaaaaaac acattccact aaatttgatg ccggttttcc ttgaggtttt gttccagcac
       61 ctcctttaat ttttttaagc tcttgatctg aacattgttt agcatttagt ctattttttt
      121 cttccattac gattccccct acaaactata aatactttta tgtcactgat tcaatatacc
      181 tatttttcta atcaaaaaat tttgagacat atttaatatt ataagtatca ttaaaattga
      241 gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca
      301 ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca
      361 gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt
      421 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
      481 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      541 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      601 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      661 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      721 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      781 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      841 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      901 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      961 tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga
     1021 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
     1081 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
     1141 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
     1201 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
     1261 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
     1321 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
     1381 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1441 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1501 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1561 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1621 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1681 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1741 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1801 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1861 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1921 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1981 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     2041 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     2101 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     2161 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     2221 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     2281 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     2341 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     2401 gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa
     2461 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2521 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2581 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2641 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2701 aggagctgga atcactaaca gtaaatttgt aattcccgac atcactgcct gtccaatcgt
     2761 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2821 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2881 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2941 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     3001 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     3061 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     3121 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     3181 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     3241 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     3301 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     3361 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3421 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3481 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3541 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3601 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3661 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3721 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3781 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3841 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3901 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3961 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     4021 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     4081 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     4141 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     4201 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4261 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4321 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4381 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4441 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4501 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4561 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4621 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4681 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4741 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4801 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4861 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4921 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4981 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     5041 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     5101 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     5161 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     5221 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5281 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5341 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5401 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5461 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5521 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5581 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5641 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5701 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5761 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5821 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5881 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5941 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     6001 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     6061 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg
     6121 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     6181 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6241 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6301 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6361 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6421 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6481 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6541 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6601 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6661 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6721 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6781 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6841 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6901 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6961 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     7021 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     7081 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     7141 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     7201 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7261 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7321 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7381 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7441 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7501 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7561 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7621 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7681 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7741 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7801 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7861 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7921 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7981 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     8041 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     8101 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     8161 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     8221 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8281 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8341 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8401 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8461 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8521 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8581 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8641 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8701 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8761 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8821 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8881 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8941 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     9001 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     9061 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     9121 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     9181 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9241 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9301 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9361 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9421 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9481 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9541 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9601 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9661 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9721 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9781 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9841 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9901 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9961 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
    10021 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
    10081 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
    10141 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
    10201 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10261 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10321 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10381 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10441 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10501 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10561 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10621 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10681 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10741 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10801 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10861 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10921 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10981 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    11041 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    11101 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    11161 ccagcgtctt tttagagaca ttcttgttct attcaggttt ttatactcct ctttattatt
    11221 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11281 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11341 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11401 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11461 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11521 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11581 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11641 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11701 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11761 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11821 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11881 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11941 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    12001 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    12061 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    12121 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    12181 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12241 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12301 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12361 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12421 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12481 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12541 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12601 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12661 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12721 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12781 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12841 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12901 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12961 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    13021 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    13081 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    13141 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    13201 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13261 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13321 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13381 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13441 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13501 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13561 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13621 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13681 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13741 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13801 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13861 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13921 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13981 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    14041 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    14101 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    14161 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    14221 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14281 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14341 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14401 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14461 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14521 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14581 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14641 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14701 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14761 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14821 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14881 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14941 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    15001 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    15061 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    15121 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    15181 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15241 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15301 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15361 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15421 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15481 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15541 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15601 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15661 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15721 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15781 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15841 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15901 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15961 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    16021 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    16081 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    16141 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    16201 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16261 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16321 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16381 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16441 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16501 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16561 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16621 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16681 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16741 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16801 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16861 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16921 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16981 tccaataaag tacataaaca taaata
//
LOCUS       DAFCNS010000058        16258 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFCNS010000058 DAFCNS010000000
VERSION     DAFCNS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.059"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..445)
                     /locus_tag="LNE23_001998"
     CDS             complement(161..445)
                     /locus_tag="LNE23_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931834.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="LNE23_001999"
     CDS             complement(463..1353)
                     /locus_tag="LNE23_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBK6931835.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="LNE23_002000"
     CDS             complement(1373..1591)
                     /locus_tag="LNE23_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBK6931836.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="LNE23_002001"
     CDS             complement(1561..3261)
                     /locus_tag="LNE23_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBK6931837.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="LNE23_002002"
     CDS             3528..4301
                     /locus_tag="LNE23_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931838.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="LNE23_002003"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="LNE23_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBK6931839.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="LNE23_002004"
     CDS             complement(5125..6096)
                     /locus_tag="LNE23_002004"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK6931840.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="LNE23_002005"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="LNE23_002005"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBK6931841.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="LNE23_002006"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="LNE23_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBK6931842.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="LNE23_002007"
     CDS             complement(9051..9875)
                     /locus_tag="LNE23_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK6931843.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="LNE23_002008"
     CDS             complement(9848..10528)
                     /locus_tag="LNE23_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK6931844.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="LNE23_002009"
     CDS             complement(10551..11489)
                     /locus_tag="LNE23_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBK6931845.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12597)
                     /locus_tag="LNE23_002010"
     CDS             complement(11734..12597)
                     /locus_tag="LNE23_002010"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBK6931846.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="LNE23_002011"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="LNE23_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK6931847.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..>16258
                     /gene="proC"
                     /locus_tag="LNE23_002012"
     CDS             15453..>16258
                     /gene="proC"
                     /locus_tag="LNE23_002012"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBK6931848.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         4759 a         3482 c         2786 g         5231 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaata
//
LOCUS       DAFCNS010000059        15872 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFCNS010000059 DAFCNS010000000
VERSION     DAFCNS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.060"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            547..1350
                     /locus_tag="LNE23_002013"
     CDS             547..1350
                     /locus_tag="LNE23_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6931849.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            1935..2390
                     /locus_tag="LNE23_002014"
     CDS             1935..2390
                     /locus_tag="LNE23_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931850.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2509..3567
                     /locus_tag="LNE23_002015"
     CDS             2509..3567
                     /locus_tag="LNE23_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HBK6931851.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3609..5558
                     /locus_tag="LNE23_002016"
     CDS             3609..5558
                     /locus_tag="LNE23_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBK6931852.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5561..9202
                     /locus_tag="LNE23_002017"
     CDS             5561..9202
                     /locus_tag="LNE23_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931853.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            9602..9940
                     /locus_tag="LNE23_002018"
     CDS             9602..9940
                     /locus_tag="LNE23_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931854.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            10050..10271
                     /locus_tag="LNE23_002019"
     CDS             10050..10271
                     /locus_tag="LNE23_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931855.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            10291..12447
                     /locus_tag="LNE23_002020"
     CDS             10291..12447
                     /locus_tag="LNE23_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931856.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            12459..12692
                     /locus_tag="LNE23_002021"
     CDS             12459..12692
                     /locus_tag="LNE23_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6931857.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            12682..13548
                     /locus_tag="LNE23_002022"
     CDS             12682..13548
                     /locus_tag="LNE23_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931858.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            13563..15533
                     /locus_tag="LNE23_002023"
     CDS             13563..15533
                     /locus_tag="LNE23_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931859.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            15700..>15872
                     /locus_tag="LNE23_002024"
     CDS             15700..>15872
                     /locus_tag="LNE23_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931860.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
BASE COUNT         5882 a         2230 c         3194 g         4566 t
ORIGIN      
        1 taaatataaa taaaagggga agctgaagct tcctcctttt cctttttttc aattattcaa
       61 agttacacaa gcaaaataaa ctcctagata gtgttagttt tttttaagat tttttatttt
      121 taagattttg aaataagaga ggagtaatcc tcctttatta aatatctgtt aatatatctg
      181 ttaaatatct gttaatatat ctgttagggg aggcttaatc tcttgggaga gtaagtatta
      241 aaggggtata aaggtaacac ttttggacag taaaggtaac aaaagtggac agtaagggta
      301 acagttttgg acagcaaagg taacaaaagt ggacatttga ggtatcacac taggataaga
      361 cgttaccttc ggttttcttg atttggaagg cgaaaaaata gaaaattaga ttagtaacaa
      421 atctttgttg actgttacct ttagttgtga tttaatttag gtaacaagca gaaaaaattg
      481 tttcctttaa ttagtaccag attaacatga ttaaaggagc gcaaaaagaa gattggagag
      541 taatcaatgt ctcaagaagt taccgaaaag attattgata gtgatgtaga tttagtatca
      601 gaacaaacaa atgcaattgt gcgttataac aatggtctga atacaacagc tttgcgagga
      661 tttaagcctg ttgaaatgga tgtgttttgg gctattttat caaaaatgaa acgtcaagga
      721 acagcagaag taacttttga tttcaacctc tttcgttatt tggtcaaata cgacagaaga
      781 gaaaaggaca gtttttattc tgtgttaaaa gatttgagtg acaaactagg taatttaacc
      841 taccgttttg aagattcaga cgtctatgaa cagctgtggc tttttcaaaa attccggata
      901 gataaacaga aggaaacagt tacaattaag gcaaatgagg aatttgaatt tatcttaaac
      961 tcaattggca aaaactttac tcgttttgaa cttgataatt ttacaaaact aagaagtagc
     1021 tacacaaaag aattgtatcg tcatctcatg caatacagaa ataaagagtg gcgaaatggt
     1081 tattgggtag tcaaagttga agattttaga agagcattaa gtataccttc aagttataga
     1141 atgacaaata ttgataaaag aatactagag caggcgaaag aagaattttt agctcctgat
     1201 gaaaacggaa tacaggtatt tgaaactttt gattatgaaa aaatctatgc aaagaaagga
     1261 agaagagttg atcggctcag attcactttt agtgagccag aatcaaactt gccaaccatt
     1321 tcaatgcata attggttaga agaagattga gaagaagcag gttgtaattt taatacaacc
     1381 tgcttcttta aaaaagtatg ggtaacactt ttggacaata aatgcaagaa gtttttttgt
     1441 aagcaagttt cagtctaaat aatatttgaa agaatggcta atctatacaa attttaacaa
     1501 ctatgggtaa cacttttgga caagtcaaga aaaaaaacaa tttttcttaa tttgttgatc
     1561 gactaaaggt aacacttttg gacaacacag aataaaaagt ccggtgatat cagttttggt
     1621 aggaaacatc ttaatatctt tgtgagattg aaggtaacat ttttggacaa gtcaactatt
     1681 tgttaaaagt taatatagag taattagata agttttttct tttcgtctga taaatggata
     1741 atattagtta ttttattgct aattagtctt tcatgatgac tgaaatagct aaaataaata
     1801 tctcgttgga taattgctga tactttaagg gatatctaaa tttgctgatt gatttattta
     1861 tgttattatg atattataat aacataaatg tgttacgccc gaacctcatt ttaaaaaaat
     1921 gaaggggtga gacaatgaat gctattaatt ggcatgaatt tgttaactat atggagagct
     1981 tactagatga tctaaaaagt tttagtattg ctgatgcagt aaagcatggg ttaccagtta
     2041 gtacaacgat gaaattacga ggattgaaaa ttccagatga aattattaag ctgagaacta
     2101 aaacaattat tgaaaccgca aaagtcgttg acaatatgaa ccatgaacta aaaggcaaga
     2161 aaacaactta cgagaaagat tttgaacgtt ttttaaaatc tgttattgga aaatatctag
     2221 gtaaaaaatc tttcagtgaa ttgagagatg aagagattgt acctactcca atggttagca
     2281 aaatccgaaa aaacaaagcc gaggggaatt actatcttgg tacaatagca aaaatcattg
     2341 attatttgga aaataaagag caaaagaaag gaacgaagaa aggaagataa tttttgtgtt
     2401 aaatattaat ggggaagaat ataccgaaat gattcaaaat cccgaaccta gaattaccga
     2461 aaaaacgtct gattgtttgc tcttttatga aagggggcag acaaactaat ggaaaaaaac
     2521 gtcaattcaa catctaccaa agatcaatac agtattattg gagggttgct tggtgataag
     2581 gataagagaa cacgtcacgg attacgcatt atgcttgcaa cttttctagg agtgcttttt
     2641 ttattaacag gcttgggtag ctacatttat caattaggaa actataatta ttatctcgtg
     2701 aacaatacaa cacctatagg ctcttcattg gagtttgctc gatcaggagc agagatcact
     2761 atttcagatg tgtggacaga tcaaaacaga gatgtaacag tagtaaaact cggttactcg
     2821 caaaacgcta gaaagttgtt atctacgcaa ggaaaaaact acaacttata tatggcaacg
     2881 aaagaaaaag atcgtcctaa aaagcttgaa atgagctatg ggatgttaag tactgatggg
     2941 gatgctttct tattcctaaa aggtaaatta gatgaaagag cttatcaagt ttttattgca
     3001 aaccaagtat cactaatttc tggtgatgat acagctgacg atgataccaa agtcaatgtg
     3061 aatgaagcag attcagttac aaaagcactt tctcaatatt ctatgaatga tgttaataat
     3121 gaaggagtaa tttcttttgg gaaaaaagca aaacctgtta atactcctga taatatcaat
     3181 ttcagaataa atccatattc agattcaact aatgtctatc aaggaagctt tcttaccaaa
     3241 gatggcgata ttgattattc aaaagttgta gcagttacaa gtaagaataa tgttgtaagt
     3301 aagttagaag atcagttaaa tgcttcaaat actaacatca agaaaattga agctacaaaa
     3361 gctgagtatc tggaacgttt acaacaaaat cctgacgata aagattcgca agaaagtatt
     3421 cgtcaactag acagtcgaat ggagaccgaa attaaagcaa tcaatgatac acaaaaattg
     3481 attgatcaat acaaaaatgc tgactttaac aaagattctt ttggagacat gaagacgaca
     3541 tttaagtacc taactctgac tgactagaag taaattattc ttattttaga ggaagtgaac
     3601 acaaacagat ggaaaatagt accgagtttt ttaagaatat tcaaactgaa tttaatcatg
     3661 ctctcacacc aatgaaacca ccgaaacata agttagtggg acgtgatgac gaactccatg
     3721 agttgtcact gatcttgaat agaccggaaa caccgcaagc tatcttgtta ggtgaagccg
     3781 gaacaggtaa aacagcactt gcaagtgaat gggctgaaag acagcgacaa gatggaaaga
     3841 aagtacaagt atacaaatta tcaatcggac aattgcgtgt tgggggagat gatgtcctcc
     3901 aaacacgttt agaaacatta attccaagat tgaaagaata tgagcgaatt ttaaaatctg
     3961 aggacaaaga aaatgaattt gtcctcttca ttgacgagtt tcataaagtt gttagctctt
     4021 ttggtagtgg caataaaatt gggggtgatt cgctaaaaga accgttagca aatgaatcac
     4081 ttcaattgat taccgctact acaagaaaag aatatgaaaa ctatatcaaa agagatgatg
     4141 ccttagggag acggttacaa ccgttagaat tgaatgaact aaaaagcgat gttgtcgtcg
     4201 atattttgag acaatggcta aatacatcct ctgttaaaga tgatcctcag gcttctttta
     4261 aattatcgca aaggattcct gatgaattac ttcaagaaat cgtacaagca aacaagcttt
     4321 atcgtgagga tttgtccgaa ccggctaaat caattgccgt aatggaggat ttgattgcaa
     4381 aagatcgagt agacggaaat gcttataatc gagaaagtct attgcaaata tttggtaagt
     4441 ataatattca gctagattct gattttgata tggaaaaact tcaagaggaa ttaaagtcca
     4501 taaaagggca accgttagca gtttatacta taacaatgtt gtttaaacgt atgaagcttg
     4561 gatttgatcg ttttaaaggt cgaaagcaac caaaagcaac tgctctgttt acaggatcaa
     4621 caggggtcgg taaaactgca atggctaaag ccgtggcaag aggactttat ggcgatgaaa
     4681 ataaagttct cttagttaat atgccggact acaaaacaaa agatgatgca atgcgtttta
     4741 gacgactcat aggtactcat gtaaggcatg atccttctgc gattattgtg tgtgatgaat
     4801 atgagaaagc tggtgtagaa acactcgatg cctttttatc aattttagat gaaggtagga
     4861 taagttttac tgaaagagga tgggatgatc gagaagttca cgacactgtt tcattgtcaa
     4921 atacaatttt atttgcgaca acaaatgcag gtgcggagat gttcgattcg ttaaacagat
     4981 atagctcact atctcgtgtt acaaacaagg aaaattctaa tgaatatagc actgaactaa
     5041 aaagagaata ccgaaaagtc gcatcaggga tcaaacaagc cttgattggt gaaggtctaa
     5101 agcctgaagt tattaaccga tttggacgaa tagttccatt ttgggcttta tccgaacaaa
     5161 ctcttctgga aattgcttat aatgaaatgt tgaagagtgc tgaattagta cgaaaacatt
     5221 taaatataga tttacgtctt ccacctagga aagaaagagt atatggtcaa aatttttatg
     5281 cagacgatct ctccatgcac ttagtttttg acatggcttc acaagaggat tctaaatcag
     5341 ggggtgcaag gaatatcttg catttagttg aatctgagtt tgaagaagcg attactgaag
     5401 caataattac ttatccaaac gcaaaaatat tcgatgttaa gttgagtgaa gccggagcgt
     5461 ttgaaggaaa aggtgattct aatgctgaaa ttattgcaaa gccttccgtt gctgaaagta
     5521 aagaaaacaa gattgtattt tcgattggag gggcgtagct atgaaattca agaaatattt
     5581 actacaaaac cttagaataa aacgatgggt cgtaagaggt ctagttattg gaggagtatt
     5641 agcagttact ttaggcgctc cttttggaat gaatgatcca actcctgaca atcaagatag
     5701 caaacaagaa acagttataa ataagaacgt tgcatatgcg gaaagtaaac cggcagaaca
     5761 aggaatgtgg acactatatg ctcgtatcct aatgggagat aaagaagtta atgatagtgc
     5821 cgctaaaact ggacaaatga atttgttagg acaaggtggt atcagtgccg atttccctta
     5881 ttctgacatt tctaaacaag gtaattcgat tgcagggggt aatgcaggta ctcaactagc
     5941 atctacttta gcaacttata gttactacgg atatattgaa acagtctcag gaaatgctat
     6001 tgccagtcaa gcatcatcca ttctaggaag tattggtagg acgattggta gttttatcgg
     6061 atcaattagt ttaatccttt ctaatattgc tgaatccata aatgaactcc ttggaaaatt
     6121 tttcatgagt atgaatatat tttctcttat ggggtgggga gaaggtggat cggatgtatc
     6181 taatccggta tctaaagcat taactagctt gcttaacaat ttgggattaa ataatgaatt
     6241 atttaaagcg atagcaagct tatctatgct ctttttcaca gcaatactag gttatcaagg
     6301 aatgaaatta ttgtcgaaag atggtgttaa ctcgaaggga ttatcaccag tacttgaaaa
     6361 atggattgtt cgtatttttg tattgtttat attcttacca gtggcaggat cgctattggg
     6421 agtttatatc aacggcatcc agtcagcaaa aaaagatgga gatataccaa caaatgttgt
     6481 ttctgactac atcattaaca caagactttg ggcttctact caaaatcttg ctccatcatc
     6541 tgatactggt ggtgatgtac ctgacggaaa gcctacatcc gaaatcaagt tcgttgatac
     6601 agattatgaa ccatctaaaa aatctgcaat gataagagcg atcaatacta gcagttatgc
     6661 cagacaaaat aatgatctag attctaattc agtcggtatg gctctattaa aagaatggtc
     6721 atcgaatgat actttcaata ttaatactta tgccggtgat gttaatgtcg ccgaaaatga
     6781 tgcttatatg ggaagagcgc ctgctgtttg gacaggctat aatttagggg atggaaaaga
     6841 aggaagtgca attaagaaac caaccaattt aaatggatat atttggtcag caaatcaaaa
     6901 tgtgacagac gacaatcgca agcctgatgg aaaaggaaca gcgcataact ttgatgctac
     6961 tcaaccttgg ggcgttgcta acaatgcaac ttttagcaca cagtcggtag ctttattgct
     7021 ccaatcggca cttgatacat cgggagctaa gttttatgcg tataatatcg ctccttcagg
     7081 aattcaaggc agtgctaaga atatgtcaac agtaaaaaca gaatggcgta cagcatcctt
     7141 agttggtcat ggagctatgg gtaagtttgg ctcgtggcta gcgatgaacg ctgaatcaat
     7201 cgttttagca gttctttact tagcatgtgc ttgggcgatg ttcaatgtga atatcgtgaa
     7261 agcatttaaa cgtgaattta ttcggatttg ggaagtagtt ctaacaggat cacttgcatc
     7321 aggagcaggt gtgatggcaa tttcattagg tgttgttaca acatctctga tcggactaca
     7381 attacctatc tttctgatta cttttattcg tgcatttggt gatactgctg tgggattcat
     7441 tcctaataca ttagttggcg atggcgtagc aaatattgtt cgatctgcta tgattttagg
     7501 tgtaggatat cttttagtct ttaaaaaagg cgaaaaagta aataatttga ttaatatgct
     7561 tattggctta ccacttgaag tagcgtttgg gtttgatgaa cgtgctaagt tattatttgg
     7621 taatagaaat gatgctgtgc gagttgtgga cggcgtttct aaagcaatca agaaaagcca
     7681 aggagctaaa aatccctatt atgatggtgg caacagctca gtaaaagata tggttgcagg
     7741 aggtcttgga gcagttggag cgcaaaaaat ggctgaacgt ttgaacaata tgggagatag
     7801 caatgctgat gctaagtcca ttgctgataa agtaattcct gaaacaccag aaggaatgtc
     7861 attcaacggt ggcggagttg gttccacaat caataatacc tctttcaaag atttagttcg
     7921 tcaagatcaa ctacgtaata actcccaaca atatattgat catttaggac gtatggttga
     7981 tgaaaacgga aactttgtcg atgaaaatgg tgaaatatta aaagatagta aatacggtaa
     8041 agatgctaaa ggcaacttta ttgacgaaaa agggcgtttg gtcgatgaag atggaaacct
     8101 tatagatact aatggtaaac cattggaaga tagtgaagat ggtaatattg gtatggatag
     8161 cgaaggacgt ttaattgatg aaaaaggtcg cttagtcgat gaagatggaa atcttgttga
     8221 cgaaaatgga cgcaggttgc taaatggtga ttttggtcat gatagtaacg gacgtttagt
     8281 tgacgaaaaa ggtcgtttag ttgatgaaga tggaaatctt gttgatgagt ttggtaataa
     8341 acttggtaaa cacagcgatg gatataatgt gaatggagat ttagtcgatg aggatgggaa
     8401 tatcattgag acgagagatg cgcttgtggc agatcgaaat agagaactcg aacaacaacg
     8461 gcatacgcca attgctgttg caattcctga gaatgaagaa gacgagatta ctttagatca
     8521 aactcatcaa gaactgagga atgaaatgag tggtgaggac gtgtcagaca gagtgcatcg
     8581 ggaattagga gatggagcat ctggtgaaag tcagtcaaga gagcttaaca gcaactttga
     8641 taatagtgct attcggaatg acacagtact taaagacaga gaatcgctaa caaaagaaac
     8701 ggataaagaa caatcagatg tgaagaaaaa gttcattcct actcctaatg ttaatcaaac
     8761 agataaatat gaaacaaata ttggatcagg aatgtcgaaa gacaatcaat caattagaga
     8821 acctgaactg acaaataaaa ataatagaac aaataattct aaatctactt cagataattc
     8881 agggaaatct aatcaatcaa ccaagaaaac atcaacagct aaatcagccg ttgaatatct
     8941 atcaaaaact ggtaaaaatg tttacacctc agcagataaa gctttagcaa aagctccaaa
     9001 aggtagtcta gcagggataa cctatgaagc taaaaacgaa gctattaaaa taggtaaagg
     9061 aacggctaag gttgccaagg ctactgctac aggagcatcc aaaactggta aagtagtgaa
     9121 gaaatctacc atgtcagtca aggactatat tgtaaaatac ggtaatcaaa aatcaactga
     9181 aaataagtct gaagacgagt aaaaagaagg agcattgatt tgctccttct ttttactttg
     9241 atttctaact gaagatgtca aggaaaattt ctatttatgc aaaaaaatta ttttaaattg
     9301 tctataaatg aattaaatat ttactgttca aagttgtata attaaaaagg gcttatgctg
     9361 gtaaagaaca gaattattat ctaaacaaaa acttgataaa ccagcatttt tagccaataa
     9421 aaaaagtgaa ataatttaat ggaaaatctc ttttaatcat cgaaaattta tgttattatg
     9481 ataacataaa gatgtaaata tcgagacgtc tttttcttag ttagatcatt gacaagtgaa
     9541 caaataagat ttgtcgatga tataattaac ttattaaaaa tattaaaaag gaggttttta
     9601 tatgttcgat tctattaaaa atttggttag cggaatgacg cctgatatga aaactttagt
     9661 acaagttgtt ttgtatttct tggcattcgt tttttcgttg aaaccaggat tcactgcaat
     9721 gaaatttttt ggtgatgcaa aatggggaga tggattaaaa tatgttgggg cgattattgc
     9781 aatctttatt attcctgtaa ttacaaccgt tatgctttac acaatgggta ctaaaacagg
     9841 taatgatttg aaccaaaaag ctaatatcat atcagcactt attccagttg ttggtatcta
     9901 tctagtttca aaatttccaa aaaatgaact gttaggttaa aagtgaattg aacaaatttg
     9961 gataatatgc cgatttgttc aatttacttt ttaccaaaaa actacgctcc aagcgtagtt
    10021 tagaagtgag ggaagaaagt aaatgtaaga tggtttttga tagagaaaaa atgttagctc
    10081 acgccaacga agtattaatg agcagtctga aaggtaccga actggcaaag ataatgaata
    10141 tgaatgttaa ccagttctat gattatcgaa atgggagtaa aaagattgaa aaagccagac
    10201 ttgaaacgct tattaaattc gaaaaagcat atgtatatat gctagataaa cagaaaagaa
    10261 ctattgactg aaaaaaggag ttttacaata atggcaaaat tcaaagatat taccgaatct
    10321 attaattatg tggcagaaac agttcctgta gttgcactct ctttgaagcc acaattactc
    10381 gaagtgtaca aaaaagaaga aaaagaagcg aaggcaaaaa aagctaaagc aaaggtatgg
    10441 aaagaattcg atgcgttgct gaccaaagat ttaccaaaga aaaattatat ggtaattgaa
    10501 gatgtacaaa agctcaacag tgagggagtt atagatgagg atgatgctat tctcgattct
    10561 gagaagtatg tcgttctatt gaagtcaagt ttgaattatg tagctgatct accagaaaaa
    10621 tttaaagaga agctattagc aggtgtccgt tttatggata gtagtggtca attattagtt
    10681 attccggatg atcctaatgt gtttgaatca gaagttgaag aagctacaga cttctatagc
    10741 gcagtagagc aattagaagt agctactaat ggattagaac gaattgctga gaccttcaac
    10801 tcttctgaag aagttgaatt tgaagccgta gcagaaaatg taccagatga aaaagaagct
    10861 ctttttgtgg atgataacaa accaaccgaa gcagaaaacc aagaagtaag catagaagaa
    10921 acacaaatac aaacagaaac ctaccaacaa ccaaaaattg atgaaaaacc cgaatcccaa
    10981 agtgacaaag aaaattatgt ttatgaagct gaaaaaatca catctgaaag tgatgtaccg
    11041 aaaaacttag atgctgattt tttagaaaaa aattttgaac agatcattgc tgacaaattt
    11101 caacctgtag ttttagatga gattaatgac cctgtcgatt taaacgagga gatgctatcc
    11161 atacctgagt tgaaaccatt gctgttttta gctaaagaga gtatgaacgc tcgcatctct
    11221 attgctaata ataaaatcaa cagtgaatta aataagacga aggagcaatt gatcctcaaa
    11281 ggaaagcaaa tgttagctga tgagcttttg cggatagagg aagcaacctc attaaatggt
    11341 caaaatcagt ttacaaatac actatcggtt gctaaagaga gctacgaagc agaagcatct
    11401 aaaattgatg atttagtgat ggaaaaatcc aatatcattc gtaaagatta tgatatggcg
    11461 aaacaggctt atttggatgc tgaactaaag aaactagagc aacggtatga tgctgagcat
    11521 ttaccagaag ttaaacaaca agaagatgaa tatagaaaag caactgaaga aagtcttgat
    11581 caacagtacc aagaagcaat tgaatctatt aaaaacgatg cattgaaaca aagccaagaa
    11641 gcaaaagaag ttgtggtgga acgaattatc aattcattga aaccagaaat tgaaaaatca
    11701 ttaacagacg tgcaagaaaa aactttagag attgtcgaaa aaacaaagtc agaaaatgaa
    11761 gctgagttcc atgatacaaa agagaaaaca acagatgtgg ttgtcaaata ttttgcgaac
    11821 aaagaagaag ttgaaaagca ggtagctaac aacacgaaag aattagaaac aaaacttgta
    11881 gagcaacaaa aaactatcga agaatataaa gatcgaatga aagaggtcaa agatttagca
    11941 gagggttata aattaaatag cgaaaagttg gcagaacaaa gtgaagcttt aagagaagac
    12001 ttatctcgtt cccgaagtga tctagctcaa atggcagaaa aaacgaaatc attagcagtt
    12061 gctacagaaa atcaatcaag ttcaaaacgt gattttacaa gtaaaaagat ggctactatc
    12121 atttctgtcg ctgcaatggc ttgcggaacg atcttgggat cgtcctactt tattagtcat
    12181 aataatcaac aagcacaggc tcaaactcaa acatctattt cgagagtgga aaattcgatt
    12241 gggacaaata cgaactctgc ttcaacaaat agtagctatg aagcaacacc aactacagag
    12301 ttcaaagttg ggaaagatgt tgtcatcagt attgatggga aaacaactaa ggctgaagtg
    12361 atttctattg atggagaaaa agcagttgtg aaaactccag acggaaatca gtacaaaatt
    12421 caagctcact tatctgataa taactaagga gggctattat gcgagaagaa aaaatagttt
    12481 atccagtaat tcaaaacaat caagaaaaca tgacctcttc tcagcactta caggaagcac
    12541 agctcgtgta tcaggtaaat gaactaccac aaacgaacga gacaatgagc aagtcaatat
    12601 ctttaattgg cttgcttatt gttgcaatag tgataactgt aggtgtttac cgtaagttga
    12661 acgtgaaagg aagtatttct gatggcagat aactcaaact atggatttaa acataactta
    12721 catcaaaaac cttctgaaac aattgtcaga gttgaatggc ttgaaaagca tgtcaatagc
    12781 acctattctg ttacagcaaa aagattaatt gtatcagcta tagttgttgc agtcgttgtt
    12841 cttggatcaa ttgtttactt agtttctgat acgattaaac tgaaatttat ttttgttcca
    12901 acgttattaa tgataattgg atttcttatt ctaattagcc agtatatgtt tattgatcca
    12961 tctagtggtt cattgatttc tggttctatt agactgttca aatcagtagt taattcagca
    13021 agagtatcta tggggaaaaa gaaaccattt cgaggcacag agattttaaa agtgaaacaa
    13081 gatggaacaa tcgtatatcg agatgggagt gtagcattaa tgttcaatct agatggggct
    13141 acatcactaa ccgcttttcc agaagaagtt atggctcagg aatcgaaatc aatacaatat
    13201 caaaatacca gagatcgttc cttagctgaa atcaaaatca cttcatctca acctcagaat
    13261 acggaaagac aaactcgaaa tatgaaaata caaaaacgaa atgcaaggag taacaaagca
    13321 gtctttgaca taatagatca gcaagaacga tttgtatcag aatatattga tggtgtgaaa
    13381 acaacagtaa tccaatatct tatcttgaaa tctcaatcta agatggtatt ggatgaaggt
    13441 gtggaacgtt tagaaaacta tacgagtaga ggactttatt atagtgtcac tcagttgtca
    13501 gctaaacaaa caaaagaaat attatctgat atttatgcac tgaaatagag gtgagagatt
    13561 aaatggaaat tatgggtaga tcacaactat taaatttcaa aaaaaagaga aaaactaaaa
    13621 aagaacaaaa aactagaaat tcaaaggtag tgaataatac ctttgttagt gatttaacat
    13681 ctttagttgg ttatcaattt acaccatctt acattaggac aggtaagcgg tggggaacaa
    13741 ttgtgaaatt agtgaatact tatggaatga atagaaatta tggatttggt tggatggtta
    13801 aagctattcc tgaagtgaga gtagaagggg taaaagccta tctaattgaa gcagataagc
    13861 cagtgtcaca aaaagatcaa caagaaataa tgcacgagaa tgttggggga tcaacgaata
    13921 gttatgaaaa taatttagaa ggaaaaaatt cagatgctga aattagcaaa actgttatgc
    13981 ggattatgga tttgaataca gcatctttac tagacagcat ggcaaataaa attattgatt
    14041 ggaagctaga aatcctatta gtatccgatt ctcctaataa aatcagcgaa cagctaatta
    14101 aactccaacg gttgtatgat gaaaaaatgt atggattaaa gcttatgtca tatggaggag
    14161 atcaacgtga tctgtttgat agacttttaa gtgaaccaat cgggaatgtc tatgagtata
    14221 cgtctatgag ttcggatttt gcaggaaatg atcatgcaat tcgcaaaggt ttagatgatg
    14281 cgaatggatg ggctatcggc actatgtcta ctagctacgc aagtggtgta gcttttatgg
    14341 cactggatga ttcttttaca agtaaagttt tagtggcatc acacaaaaac gctacaatta
    14401 aaggctatga tgaaaaaatc tcagcatcat cactttgggg gcaattgatt gctaataatg
    14461 caatggcaaa tcataaacga acttttcata ttgtcttaaa taaatttgat tactttggtg
    14521 atccaactgg tagaacattt gttgccaaac cttcgttaaa cagtgtaata gaaaaggtta
    14581 atttggctga agaaggtctt aactctttgg agctttttgg taatcaaaag gacattggtg
    14641 atttatatag acggaatctc gataaactaa ctcagatttt ttatttgtta gcaggcagaa
    14701 cattagattc tgcaactaga gttttattga aaaaagaatt aggtaacttt tatataagtc
    14761 agaaactttg ggatattaat tatgaagaac gttcaaaact tttggtgaac agtagtgata
    14821 caatgccagt tcttggggat tttattagct atttgactaa tgctgaaaca aaagccaaaa
    14881 aatctggtac tgaaaaagaa gtcgatcgta ttattgtatt aaaagacaca ttgagaacag
    14941 cattagacag cttctctagg atgtttaatc aaagaacgac actgaaagaa atcggtaaaa
    15001 gtgacaaatt acagttttac tataacttag gtgatctaag agcagaaacc aatgttatgg
    15061 aagctcaatt tttaaatacc tttgatttca tcacatcaca agcaaaaaaa gatgatattg
    15121 ttatgattca cggcttcgat aagttatcta ttgaaaccgt tgggctaatt aaaaaaagat
    15181 taacaatgct tgaagaagat ggaattcgtc ttgcctttct atttgataca atcggttcag
    15241 gagaagacaa tgaatacaaa gaagatattg aaacagctga tatattcaat acagatggaa
    15301 aaatttacca aagttttgaa agtgatttta attacactat cattggaaag atgaccagtg
    15361 gagagttaat gaagtatgaa aaaaaagttg ctcctgctag aaaaggagaa gatgatcaaa
    15421 aactttctgt tgatttgaga cagtatttga caacaggaag aagtaataaa tttcagatca
    15481 ggagaccagt ggatctgact aataattttg ttattggaga gtttattctt taagttttat
    15541 ctttgatttt gagtgtataa ggaaaggata agaaaaaaca tgtatatttg agactaaaaa
    15601 cagtcttaga gaacatggta cgttttgtga aaagagaact atagaaattt cataaagtga
    15661 aaccttactg agtgaaataa ttatagtgga ggttaaataa tgaaaccaaa cataaaaatt
    15721 ttagatcgaa tatttctagg tagagacaca gaagtaatcc taattcaaca tgaagaagga
    15781 tttgaagtta gtataggtat tcagaaactt caaaaacctc attattgtaa tcagttgtat
    15841 aaaaacttta ctgatgaaga aaaagcgaga gt
//
LOCUS       DAFCNS010000060        15329 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFCNS010000060 DAFCNS010000000
VERSION     DAFCNS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.061"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..251)
                     /locus_tag="LNE23_002025"
     CDS             complement(21..251)
                     /locus_tag="LNE23_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931861.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="LNE23_002026"
     CDS             complement(687..1253)
                     /locus_tag="LNE23_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6931862.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="LNE23_002027"
     CDS             complement(1253..2209)
                     /locus_tag="LNE23_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBK6931863.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="LNE23_002028"
     CDS             complement(2209..2865)
                     /locus_tag="LNE23_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBK6931864.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="LNE23_002029"
     CDS             3235..5253
                     /locus_tag="LNE23_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6931865.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="LNE23_002030"
     CDS             5680..8094
                     /locus_tag="LNE23_002030"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBK6931866.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="LNE23_002031"
     CDS             8442..9995
                     /locus_tag="LNE23_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBK6931867.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="LNE23_002032"
     CDS             complement(10376..12160)
                     /locus_tag="LNE23_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931868.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="LNE23_002033"
     CDS             complement(12175..13896)
                     /locus_tag="LNE23_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931869.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="LNE23_002034"
     CDS             13996..14757
                     /locus_tag="LNE23_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6931870.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4991 a         2801 c         3004 g         4533 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttat
//
LOCUS       DAFCNS010000061        15229 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFCNS010000061 DAFCNS010000000
VERSION     DAFCNS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.062"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            278..787
                     /locus_tag="LNE23_002035"
     CDS             278..787
                     /locus_tag="LNE23_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6931871.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="LNE23_002036"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="LNE23_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6931872.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="LNE23_002037"
     CDS             2322..3302
                     /locus_tag="LNE23_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6931873.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="LNE23_002038"
     CDS             3424..4842
                     /locus_tag="LNE23_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBK6931874.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="LNE23_002039"
     CDS             4913..6382
                     /locus_tag="LNE23_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6931875.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="LNE23_002040"
     CDS             6392..6859
                     /locus_tag="LNE23_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6931876.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="LNE23_002041"
     CDS             6895..7767
                     /locus_tag="LNE23_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6931877.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="LNE23_002042"
     CDS             7848..10382
                     /locus_tag="LNE23_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6931878.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="LNE23_002043"
     CDS             10538..11311
                     /locus_tag="LNE23_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6931879.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="LNE23_002044"
     CDS             11304..12095
                     /locus_tag="LNE23_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6931880.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="LNE23_002045"
     CDS             12105..12596
                     /locus_tag="LNE23_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6931881.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="LNE23_002046"
     CDS             12614..13024
                     /locus_tag="LNE23_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6931882.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="LNE23_002047"
     CDS             13028..14428
                     /locus_tag="LNE23_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBK6931883.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="LNE23_002048"
     CDS             14451..14825
                     /locus_tag="LNE23_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6931884.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2134 c         3045 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactccta
//
LOCUS       DAFCNS010000062        15138 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFCNS010000062 DAFCNS010000000
VERSION     DAFCNS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.063"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..2377
                     /locus_tag="LNE23_002049"
     CDS             5..2377
                     /locus_tag="LNE23_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931885.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="LNE23_002050"
     CDS             2438..3898
                     /locus_tag="LNE23_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBK6931886.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="LNE23_002051"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="LNE23_002051"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK6931887.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="LNE23_002052"
     CDS             6129..6278
                     /locus_tag="LNE23_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931888.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="LNE23_002053"
     CDS             6353..6946
                     /locus_tag="LNE23_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBK6931889.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="LNE23_002054"
     CDS             6959..7858
                     /locus_tag="LNE23_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBK6931890.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="LNE23_002055"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="LNE23_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK6931891.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="LNE23_002056"
     CDS             8724..8984
                     /locus_tag="LNE23_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931892.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="LNE23_002057"
     CDS             9429..9635
                     /locus_tag="LNE23_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBK6931893.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9683..9886
                     /locus_tag="LNE23_002058"
     CDS             9683..9886
                     /locus_tag="LNE23_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931894.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="LNE23_002059"
     CDS             9902..10339
                     /locus_tag="LNE23_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931895.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="LNE23_002060"
     CDS             10332..11039
                     /locus_tag="LNE23_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931896.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11419..11598
                     /locus_tag="LNE23_002061"
     CDS             11419..11598
                     /locus_tag="LNE23_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931897.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="LNE23_002062"
     CDS             11923..12186
                     /locus_tag="LNE23_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBK6931898.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="LNE23_002063"
     CDS             12180..12530
                     /locus_tag="LNE23_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK6931899.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="LNE23_002064"
     CDS             complement(12554..13168)
                     /locus_tag="LNE23_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6931900.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13482..13904
                     /locus_tag="LNE23_002065"
     CDS             13482..13904
                     /locus_tag="LNE23_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931901.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13914..14117
                     /locus_tag="LNE23_002066"
     CDS             13914..14117
                     /locus_tag="LNE23_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931902.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14328..14912
                     /locus_tag="LNE23_002067"
     CDS             14328..14912
                     /locus_tag="LNE23_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6931903.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5570 a         2451 c         3061 g         4056 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt
    13321 ttaaatggca atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca
    13381 ttaaaaatat attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt
    13441 aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
    13501 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
    13561 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
    13621 ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
    13681 taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga
    13741 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
    13801 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
    13861 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
    13921 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
    13981 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
    14041 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
    14101 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
    14161 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
    14221 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
    14281 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
    14341 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
    14401 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
    14461 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
    14521 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
    14581 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
    14641 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
    14701 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
    14761 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
    14821 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
    14881 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
    14941 gccatttatc tagaaaagtg acaatagtat gagaattttt tgtaagtgtc tactgataac
    15001 ttattctcaa atgttgtaag gattttattc atttgttaat attaaagaac ggaactccaa
    15061 aaaagtccgt actctaattt gcagtctctc actcttttag agtgagagtt tttattagat
    15121 tttcaaatta attagtaa
//
LOCUS       DAFCNS010000063        14987 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFCNS010000063 DAFCNS010000000
VERSION     DAFCNS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.064"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(116..1246)
                     /locus_tag="LNE23_002068"
     CDS             complement(116..1246)
                     /locus_tag="LNE23_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK6931904.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1787..1993)
                     /locus_tag="LNE23_002069"
     CDS             complement(1787..1993)
                     /locus_tag="LNE23_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931905.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2009..2623)
                     /locus_tag="LNE23_002070"
     CDS             complement(2009..2623)
                     /locus_tag="LNE23_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBK6931906.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2770..3453)
                     /locus_tag="LNE23_002071"
     CDS             complement(2770..3453)
                     /locus_tag="LNE23_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK6931907.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3562..4020)
                     /locus_tag="LNE23_002072"
     CDS             complement(3562..4020)
                     /locus_tag="LNE23_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBK6931908.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4104..5867)
                     /locus_tag="LNE23_002073"
     CDS             complement(4104..5867)
                     /locus_tag="LNE23_002073"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBK6931909.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6088..7548)
                     /locus_tag="LNE23_002074"
     CDS             complement(6088..7548)
                     /locus_tag="LNE23_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931910.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7756..8682)
                     /gene="glsA"
                     /locus_tag="LNE23_002075"
     CDS             complement(7756..8682)
                     /gene="glsA"
                     /locus_tag="LNE23_002075"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBK6931911.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8705..10132)
                     /locus_tag="LNE23_002076"
     CDS             complement(8705..10132)
                     /locus_tag="LNE23_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6931912.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10163..11377)
                     /locus_tag="LNE23_002077"
     CDS             complement(10163..11377)
                     /locus_tag="LNE23_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBK6931913.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            11727..12806
                     /locus_tag="LNE23_002078"
     CDS             11727..12806
                     /locus_tag="LNE23_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6931914.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(13107..13349)
                     /locus_tag="LNE23_002079"
     CDS             complement(13107..13349)
                     /locus_tag="LNE23_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6931915.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(13381..13938)
                     /locus_tag="LNE23_002080"
     CDS             complement(13381..13938)
                     /locus_tag="LNE23_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6931916.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(13951..14139)
                     /locus_tag="LNE23_002081"
     CDS             complement(13951..14139)
                     /locus_tag="LNE23_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBK6931917.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(14152..14718)
                     /gene="amaP"
                     /locus_tag="LNE23_002082"
     CDS             complement(14152..14718)
                     /gene="amaP"
                     /locus_tag="LNE23_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBK6931918.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         4447 a         3172 c         2599 g         4769 t
ORIGIN      
        1 tatgattttt tgagatactt tcattgcgaa ttcctctttc taaaagaacc tctcgcttga
       61 aaaataaaag attgtacttt atgataattc aaactgagag tttctcagtt tctctttatt
      121 gtttttcagg tcgatagagc gccaactctt cggcctgtat ctttgaagaa atattcaacg
      181 tgattccgat tcctaaagaa aggatcagat aacttgttcc tccgtagcta acaaatggca
      241 atggcacacc agtcattggc atcagccccg cgatacttcc cacgttcatt atcgtctgaa
      301 cgaatagcaa agttccgaat cctaaaagaa acaatccagc ttgctgattt ttacagcgac
      361 tgcttaaaca aaagattctc atgcataatg agaacaataa gaaaagcaca cacaatgcac
      421 cgattagccc aagctcttct gtaataatcg aaaaaataaa atctgtttct gtctctggaa
      481 gatacccttt ttttgtgata ctatttccta atccccgccc aaatatgcca ccattatata
      541 gtgcataaaa cgaatgggtc atttgatagc ccgcaccatg actatcgata aaagaatctc
      601 ttaaagtcgc gatcctttca taagcatgat taaacatctg tggaagccaa cctttatttc
      661 ctaaatataa taccccgcct gctgctccaa tcagcaatgc agaaaaagta acgattagat
      721 agatcccttt tttaaatgga accgccgctg cccaaaatat gacccacgca atcgaaagga
      781 tcatcaatgc tccagcaatc tttggctgaa ataaaatcag aaaagttata cctactgtaa
      841 tcaggaatgg tttttttaga tcttttgggc tactatttcc gtcacgaaaa aaccagctta
      901 aataaaaaat caaaaaaaga tttgccagtt cagaaggctg gaattgaatg ccaaacagag
      961 agatccaacg ttgtgcgccg ttgacagtga caccgaatat ccctattctt actaatacta
     1021 aaaagaaaat cgataaggct aaaccgtatc ctgctatttt agggtgaagt aaatagtgta
     1081 gtttgactga acgagctaag aagatcacgc tccaactcaa aaatatgaaa attagttgac
     1141 gcaataatag actttttgta ttttcatcgg cttgcaataa acgataagaa ctggcactgt
     1201 atacttctaa aagaccgatc atggacaaag tcaaataagg acccaaaatc caccaatcga
     1261 tcttttttca ccgcatctcc tcctcacaca gcaatccact tgtcaggtga ggagcgagtt
     1321 tgtctcatca aataagtcag tcacaaaaat ataaagaaaa ggtcagaaac cttttcgaac
     1381 aagaattctg acctagcatc atctcatcgt ccgagtttta gcactataca acgtaaagaa
     1441 aacattctta gctattggaa aaagccttaa tccaacagtt tctgttttac ccattggcga
     1501 ctttcgacgt ttctgggcaa cagcctatgt ttgtatagga gcctcaccta acaaggttat
     1561 tactatattc tatttacgag ttcatgatag acttttccta atgattaggc aatggaattt
     1621 gaaatgaaat agtgaaattt aacaatcttt aatatttttt agaaaaaaat aacaaataag
     1681 ttgaaagaat cccgttgcaa attctttcag ttgcaccagg attctttgat aattttttag
     1741 ttttatgtgt ttttttatat gttttttcac ttttcggaat aaatatttat ttgttaaagt
     1801 aattaaacaa tccgctaatc gtttgatctg gcatttctat ttccttacca tctaaagtaa
     1861 cagtagtaac gacaaaatgg tcttgcaatg gcggaacttc taagtactgc ccttgcagaa
     1921 aatcattcac tgtttcatat tcttttttca tttcttttga atctgtcgat ggtttatagc
     1981 ttatttttaa cataactagt gctccttttt attcgggacg tgcaatcgtg aaaatttctc
     2041 ctattgctgc ataatcattt cctgccaaac ggcttacagc attcaatttt cttggatcga
     2101 tccttcctgt cttatcatca tagactgttt catttaaatg atatttctgt acttctagca
     2161 aaaataaatc agctgttact ttatttttat tccgtatctc gacatgttca tataaggatg
     2221 tttcaaaacg aacttttgct tctgtcaatg ctggtactga aatgattact gaatcagtta
     2281 aagtcaagtc agtcaggtac agttcgcttt cctcattagg cagattggct gctgttttat
     2341 ttacttcttc tacgttgtcc tcatcaagga tatggataac agcttctctt gtctcaagaa
     2401 tattctttgc tgtgtctttt actgcatctt gttttctctg gatcgccaag gataagatgg
     2461 gtcgattcga actgacgata ttgaagtaac tgaagggtgc gatattcact atatcttttg
     2521 ctgatttagt cgttactact gctactggtc tagggatgac tgaaccaatc agcaacttgt
     2581 aattttcacg ttctgtcaat tctgaaggac tccgttcaaa catggtttcg ccttctttct
     2641 ataaaattcg ttaaaaagca gatcttgaag acaaaatgaa aagtaatgct tttttcatct
     2701 tcttagaatc tgctttaaac tatctatcaa tttttctttt ttgtcaagaa tctgtgccgt
     2761 gcgtgttttt tagttatttg ctttacttgc ggcttcgaca tatcttttag tgatcaattg
     2821 cggacgggtg ataatagatc caactacgac tgaaaaagca cctaattcat atgctttctc
     2881 tacttgttca ggagagtcag tatgtccttc cataattact gggatatcga cttgttctat
     2941 gatttgattc aataaatcaa aatcaggtgt ctgcgtcttt cgtgaatatt ctgtatagcc
     3001 tgctaaagtc gtagacaaaa gatcaaaacc atcttcttgc gcttgaagcg cttcctcaaa
     3061 agtcgaaata tctgccatag ctaaccgacc gtgttcgtga atcatttgga caagttcagc
     3121 agttgttttt tctgtcggac gtttacgaga agttgcatcc agtgcaatga tttctgcttg
     3181 cgtctccaaa agttcttcca cttctttttc tgtcgcagta ataaacactt ctgacccatc
     3241 gtaatctctt ttgataatcc ctattaccgg taaatcaacg attcgcttga tttcattgat
     3301 atcttctgtc gtatttgctc gaataccaac tgctccgcct tcttttgcag ccaaagccat
     3361 tctccccata ataaaagaac tgtgtagcgg ctcgttttcc aaagcttgac aagaaacgac
     3421 gactccgcct ttgatttgtt ccagtgtatc cacaatatct cctccagtaa tgttatataa
     3481 aaatacagct tcatataaaa atgtaaccct tttctattca ggagacaata atgagctatt
     3541 tctttcagca gacctttctt actaaaagaa aattacttca aattcttcgt atacgaccag
     3601 cttctcttct gtaaattcta tgttcaacgc ttctttataa gcatgtttaa aaaactcagc
     3661 atggactttc cgccaataag ctaatgtccg atccccttct ccttctttcc aagcatgctc
     3721 tttcgtgact tcactaaaag gcaagatggt cacatttata tttttgatga tacaaactgg
     3781 ttcatttttt ccattcagga ccacgtctaa ttgaccaact tttggtaaag gctcattctc
     3841 ttgtttatac agatcgtaac cacttgatgt accagtcttt atctttttta caactagttc
     3901 gctcaactcg tctgccattt tttctgtatc cccaaatgaa aagaaaccta atttttctga
     3961 ttgataacta ggatattttt cttggaactt tttgacatat tcttctactt gctgatacat
     4021 aacatccacc cctttgcttt ttataatgaa aaaaccatcc agatactagt ataaacaatt
     4081 atctggatgg tgagcataat gaattattga atattcaaga cacgcagact atcttcaacg
     4141 atttgttttg gtgttaaatg ataacgttca taaagctgcc ctacaggaac gctatccgtg
     4201 aattctttct ctgcaccaaa atttaatacg ttcattgaag aatcaccata ataacgactg
     4261 atattttcgc caaatcctcc tgacaaacta ccatcttcta atgtgatgac taattcgtgt
     4321 gtttctttta atttatctaa tgtttctacg tcattttctg tgatcgttcc tggattgatc
     4381 aacgttgctt cgattccttt ttttctaaaa gcttttactg tttcttttcc taattcaaga
     4441 aaaccgccta aagctaaaat tgctactttt cctccattat ttactgtttg ataggctgga
     4501 atactataat catttgcgat agctgtacca tgttctacac catgtgtagg aattcggatt
     4561 gctacaggct gcgctgtttg ttcgaacgcc caatctagca tagagatcat atcctctttt
     4621 gttgctggag caagatattt gatgttcggg atacttgata agtaaggaat gtcaaatgtt
     4681 ccttggtgtg ttcggtcact agctgagatc gttccgccac cgactaaaat aatcactgga
     4741 ttctcgttga tcgctaaatc atgtgataat tgatcatatg cccgttgcaa gaaagtactt
     4801 gattggaaga cgactggacg tactccctga gaagccaaag cagaagcgaa agtgatagag
     4861 tgctgttcag cgatccccac gtcaaaataa tgatctggat gtttttcttg gaatgttttc
     4921 agtccaaagg cgcctggtat cgctgcattg actgcggcaa taggagctgt acttgtttgc
     4981 attttcttat ccaaataatc catgatgatc atattaaatg tttctccaga agttacttgc
     5041 tttgcctccc cggtggacag atcaaatggt acatgccaat gatatttcat tttattatcg
     5101 attgccggct gataaccgcg acctttttcc gtatggacgt gaagtacaat cggatgatca
     5161 atatctttta cttcttcaaa taaacggatc accgtttcca gatcgtttcc attatccacg
     5221 taacgataat caaatcccat tgctttaaat aggttattgc tgcttttacc gttggtttga
     5281 cgaagctcag ctaagttttt atataaactg ccatgattct catcaataga catctcatta
     5341 tcgtttacta atacgattag attggattgt aaaacaccac cattgttcaa tccttcaaaa
     5401 gctaagccac cacttaggga gccgtcaccg ataaatgcta ccacatttcc tgattctttc
     5461 ttcagatctc tcgctttagc taaacctgtt gctaagctga tagatgtaga tgtgtgcccc
     5521 actgtaaaat agtcatgttt actttcatgt tggttggaat atccgctcac ttcacggtaa
     5581 tgtccttttt caaaaccatt ttttcgacca gttaaaagct tatgaccata agactgatgg
     5641 gaaacatccc aaacgatttt atcctttggt gaatcaaata cataatgaaa cgcaatcgtt
     5701 aattctacta cacctagatt cggaccaacg tgcccaccta catcgctgtc cttttgcaag
     5761 attttttggc gcatctcttc tgccaactgt ttcatttcct caacagataa ttttttgaca
     5821 tctgcggggc tattgattgc atccaataca tgtaatactg tactcataat acattctctc
     5881 ctccgtacta tcttctttta atgaaaatag atttgttaca ctttatctat tctactactt
     5941 agagttgact ctaagtcaat agcattttaa aaaaatttca gagaaagctt gttatataag
     6001 tagatcttcg cacgaatcag gactcaaata aaaaccttct acagacaatt aaaaagcttt
     6061 gtagaaggtc tcctaattaa ggtctcatca agaaatatgt ttttttaata ggtgaagttc
     6121 ctgagcaata tgatacaaat ggatgtttgc accttttaat gagattggat agatctttgt
     6181 cttttcagtt tctaatccat tttgatagct agcagcatcg cacaaaacca ctcttttttt
     6241 cgcacataga cattcatcag gcaattcttc taatgaacga aataaaatag gatttatttc
     6301 agcgatgcct tcttccgcat acaaatgaaa ttgggttcga taaaaaaatt gtttgactga
     6361 actttctgaa acaacaaaaa tttctatttc ctgtctcccc gcttgttcac tgttgatcaa
     6421 agaaagactt ttgacgaatt ttcgaatgaa attttcattc catcttagtt ggatttgatt
     6481 tttctttttc cactcttcca atatttctac aatttttccg tacagaagtt gttctttttc
     6541 tgatagaaga tagatccttt ccggtaaaaa tacttgtcca tctgcccaca acgtacggac
     6601 aaattgtaaa aaggattgct tgaaaataaa attttctgca tcgtcgattt cagcgatact
     6661 aataatctgt aaaatcaatt cattcagtga taggtcattg agaacaaaat ttttataaac
     6721 aacttcgaaa tcttttctta acagctccat gttctcattg gtataatttc tactgttgaa
     6781 caaagaaaaa atataataga catcttcttc ttgtaagtct agaattcctt tttgctcttc
     6841 caatccttga cagactaatt gaaataacgg cttcgtttct gcgcttttct tatcttcttc
     6901 tttcagtcca aggggaaatg cctttcttcc gattgcgatc tctattcctc gtatgataaa
     6961 ggacttttca tcttccgaat atcttctttt aaaaaaatga gtctctacat aatcaatcaa
     7021 ctcgttacaa gcttcttcta catacggatt tttattttca tagccttccc aattgacata
     7081 acgtactaaa gctagcaaga tgttccgcga gtcaaattct gggataatga tttctttatt
     7141 tttcaaataa tcattttctt taaaaaaatc caatacgatt tttttgattg tatatacttt
     7201 gctcaaagaa ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata
     7261 ataacttaaa aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag
     7321 agcaatacca tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat
     7381 acaatattct agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac
     7441 ccttagagaa atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca
     7501 cagatacact tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg
     7561 cctccagttt ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt
     7621 tttatttaga aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac
     7681 aagctcacaa tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc
     7741 ctctttcaag aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa
     7801 caattggata ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat
     7861 gccgtaacga tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac
     7921 accaacatgg acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga
     7981 tttgacgatt gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc
     8041 tttatttgcc agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca
     8101 ttgtctgaaa taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt
     8161 catatagtaa gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc
     8221 ttcattcaat gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc
     8281 aaatttttca tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt
     8341 aacaaaagga ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa
     8401 ggcaaaaccg gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt
     8461 gattgctaat aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc
     8521 gtctcctccc tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt
     8581 gggatccact tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg
     8641 gaaatttttt ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca
     8701 ttttttattt ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa
     8761 ctaagacgat aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg
     8821 cgatactagc aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta
     8881 aacaagcaac gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac
     8941 tgtcatcttt aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt
     9001 agattacaac agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta
     9061 atgtaaagac ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag
     9121 gaacaccgtg tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta
     9181 cgtgaagtgc ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac
     9241 aaacaagaat cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata
     9301 ggaattctaa cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag
     9361 tagcgacggt caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca
     9421 ttgctaatgg ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt
     9481 ctacgcccat atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa
     9541 aatcaggaat caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat
     9601 aatggatccc taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga
     9661 tccgagcaat caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga
     9721 aaataatcaa taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg
     9781 ttgcatagga taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct
     9841 caaaccattg gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc
     9901 aagtatagac accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg
     9961 ccacagggat aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg
    10021 aggtcgcgaa tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa
    10081 agccaaagaa cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc
    10141 ctcttttcta tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc
    10201 cttcttcttg ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa
    10261 tcactgttgt cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc
    10321 ctaagaagta ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta
    10381 tcagaatcgt acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat
    10441 cccattgttt ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc
    10501 caatcagcat ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc
    10561 cagcaacgga tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa
    10621 tgatcagcca agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga
    10681 ctgcttgttc atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa
    10741 gcagtcctgt accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag
    10801 gaatctttct ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt
    10861 ggatgactgc tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc
    10921 atatccagtg gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat
    10981 agccttttaa attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga
    11041 taaacatcag ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt
    11101 tggggaagct tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc
    11161 cgatatcccg tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc
    11221 caacggagag aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc
    11281 cgtaaaaaaa agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga
    11341 atgctgtatc tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat
    11401 tacgagtata aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc
    11461 atttataaaa gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca
    11521 cacaaataaa gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct
    11581 ttttttattt ttaaagatat tttttcataa ggaataaaga ggcggatttt ataattaact
    11641 tagccatttg gaaataaaaa aacgccacgt gaattccatg ttatattgaa gttaccacaa
    11701 ctctcaatag acaaggatga attcacatga cgcaaaacca tgataacaca gtttcacgca
    11761 agggaaaaca cctaacctac tcagaacgtt gccaaatcgc catcctgaaa aaggaaaact
    11821 attccaaccg tcaggttgcg gatgtcctgg gctgcgtgcc gcagaccata aacaacgaaa
    11881 tcaaacgcgg gaccgttact cagcttaacc gccagaatca gaacgggaag gtctacgact
    11941 attatcatga cgtctacgac gccgatgccg ggcaggcggc ctacgagaag aaccgcttga
    12001 actgcggcag acggccctta tgggccgaca cggacgcctt cattgaatgg gcggacgaca
    12061 tgatgctgtt ggaaaaatgg tccccggacg tggtggtggg ctttgccaga aaacacgaat
    12121 tgttcgaccc ggccattatc cctgggacca ccaccctcta tcagtggatc gaccgtggca
    12181 tcatgaaaac gaagaatatg gacctgctag aaaaactgtc ccgcaaaccg aaatcgtccc
    12241 gtcatggcaa ccgcccgaat aaacgggtgc tagggaaatc cattgaagag cgcccggagg
    12301 cggttggtac ccggaaaacc ttcggccatt gggaagtcga cacggtcgta ggaaacaaga
    12361 ccaagtcgga tgccgtgctg ctgacgttgg tggaacggca gacacgcttc gaggtcattc
    12421 tgaagctggc cgggaaggat gccgaatcgg tcaaccaagc ggtccgacag ctgagagaac
    12481 gggctggtgc gtacttcggc cagctgttca agacggttac ctcggacaac ggctcggagt
    12541 tcgcgggact gcacgatgcc ttgcaggatg tggcaaacat ctacttcagc cacccctatg
    12601 cttcctggga gcgcgggacg agcgaaaacc aacacaagct gattcgccgt ttcatcccca
    12661 agggcagccc aatcggcaac gtgagcgaaa accaaatctt gcggattcag cgttggatga
    12721 acgattaccc gcgaaagatt ttgggctatg caacgccgca tgacacgttc gtccaggcat
    12781 ttaggcagga acggttgacg gcttgacacg tgggcccttg acaacgagcc tccctcggca
    12841 tggcttgtgt ctggaaagct ggccggggcc agccagtcgg gagactacca tgcctcagct
    12901 cattgtcaag ggcccactcg attagaaaaa ttagacggtt ggtggtaacg agcaaccggg
    12961 tcaccatggg tggctaactt aaacttgaaa tttacgtaag gaataaagaa tacttcatat
    13021 aaaatatata aaaaagaacc gcagcgagtg tcgctacggt tctcaaattt gttcttcatt
    13081 tcaacataag tttttcttca atttgcttat aatttacgtg caaagaatga aacgactaat
    13141 actaaaatga ttgcaccgat caatgatgga atgatagcca ttcctgcaat tgttggtccc
    13201 caagttccta gtaaagcttg tccaattgct gaacctacta aaccagcaat gatgtttgcg
    13261 atgatcccca ttgatgatcc tttattcgtg attgctcctg caattgctcc aatgataccg
    13321 ccgacgatta atgcccaaat aaatcccatt ttaaattcct cctaactgac ttcttacttc
    13381 ttattgtaca cgtggttcgt tattttcttt gactttttgt gcacctttgc ctacagcaga
    13441 ttttgcttta tctgtttggc gtgaagcaaa ttgtcctgtg gattttgctg cttctgttac
    13501 tttgtcttgt actgtttctt gatctttttg gtattcttct tgtgttttga tgtctgcaac
    13561 attcacattc acttcgataa catctaaatg tgtcatattg tttacttcag tactgatcaa
    13621 ttctttgatt tggttgtaaa tatcttcgat gtctttaccg tattctacga tcacatctag
    13681 atccacagct acttgtttct tacctacttc tgtattgata cctgctgtta cgttatctgt
    13741 gttgactaat ttttcagcca tatttgagaa aaatccgcca tctaatgtca atagaccatc
    13801 tacatctttc aatgcataac cgatgatttt ttggatgact ttgtcttcat aagtcaattc
    13861 accattcatc atgcggtttt ctcctttttc atttgccaaa actgatccat ttgttgtatt
    13921 catgtttttg ttttccatct ttgtttcctc ctatgattta aaaaattgat caactattcc
    13981 tgactgtttc aaataaaaac cgattcctgc gccaattgct gtcatgatta tgatcaacaa
    14041 tgtcttgaaa aatccaatcg ttaaccataa aacagctagc agtagtccaa gacccccaaa
    14101 gataataggg agcttgtatg ctgaaaatag ttctttcatt atttggccct cctattccac
    14161 tcgagagtgc ttgtagtctc ttgtatcttc ttgttcttca taaccgctgt atgtgactgc
    14221 cactttgacc gtttcttttg ttccgagtac tttagttact tcttcttcga tatcttgcat
    14281 caatacacct gttttcccta ctaatgaaga agttcgtttc aagtctccgt tgacatggat
    14341 atggattcga tttttcgttg aacgtatccg tactttaggt gaatgaatga actcttcttc
    14401 gtgtaaatgg gaacgtacca acccttcgat cgcttttttg tcgattgtta gtttgccatc
    14461 ttcccgtttc ataacgaaag ttcctcttga ctttggatag aaaagaacga ctagcaagat
    14521 gataatagca agaacggcaa atactacagc tgcccagaaa atatattgtt gcatgtagta
    14581 attcgttaaa gtaaagaagc gaaatcgttc caattgttca ggcataatat acggcgcatt
    14641 gatcaacgca atcgctagaa atactgaaag tagcagtaaa ctcatgataa caccgattat
    14701 ttttaacccc ttattcattt tccacctcct ataaacttat ttttaccctg taaataactc
    14761 taccgacaat cacagtatag atagcgaaca gttagcatag catcagtaaa taaagcgcta
    14821 ccaaacattg ctggatcata acggcgtttt attgccagtc tgatactgaa ataacaattg
    14881 cttacttatt ctctatttac ttttgattgc tggctatact taaaggatat tattcaagat
    14941 taaaaattgc aaagattatg ctcagttatt attacaagat tctattt
//
LOCUS       DAFCNS010000064        14800 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFCNS010000064 DAFCNS010000000
VERSION     DAFCNS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.065"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            145..402
                     /locus_tag="LNE23_002083"
     CDS             145..402
                     /locus_tag="LNE23_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931919.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(634..1065)
                     /locus_tag="LNE23_002084"
     CDS             complement(634..1065)
                     /locus_tag="LNE23_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBK6931920.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
     gene            complement(1416..2108)
                     /locus_tag="LNE23_002085"
     CDS             complement(1416..2108)
                     /locus_tag="LNE23_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK6931921.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(2545..3114)
                     /locus_tag="LNE23_002086"
     CDS             complement(2545..3114)
                     /locus_tag="LNE23_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK6931922.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(3188..3511)
                     /locus_tag="LNE23_002087"
     CDS             complement(3188..3511)
                     /locus_tag="LNE23_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931923.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3665..4477)
                     /locus_tag="LNE23_002088"
     CDS             complement(3665..4477)
                     /locus_tag="LNE23_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931924.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4831..5679
                     /locus_tag="LNE23_002089"
     CDS             4831..5679
                     /locus_tag="LNE23_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBK6931925.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5824..6765
                     /locus_tag="LNE23_002090"
     CDS             5824..6765
                     /locus_tag="LNE23_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBK6931926.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6755..8926
                     /locus_tag="LNE23_002091"
                     /pseudo
     CDS             6755..8926
                     /locus_tag="LNE23_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(9038..11209)
                     /gene="copB"
                     /locus_tag="LNE23_002092"
     CDS             complement(9038..11209)
                     /gene="copB"
                     /locus_tag="LNE23_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBK6931927.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(11229..13415)
                     /locus_tag="LNE23_002093"
     CDS             complement(11229..13415)
                     /locus_tag="LNE23_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBK6931928.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(13415..13624)
                     /gene="copZ"
                     /locus_tag="LNE23_002094"
     CDS             complement(13415..13624)
                     /gene="copZ"
                     /locus_tag="LNE23_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBK6931929.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13637..14077)
                     /locus_tag="LNE23_002095"
     CDS             complement(13637..14077)
                     /locus_tag="LNE23_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBK6931930.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(14151..14690)
                     /locus_tag="LNE23_002096"
     CDS             complement(14151..14690)
                     /locus_tag="LNE23_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6931931.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         4285 a         2991 c         2564 g         4960 t
ORIGIN      
        1 atttttctgt ccacgttcat tttactccaa agttttcctc atttcaaaag aaaatcacag
       61 aatacacgaa gaagcgtttc tatttgtata aactatatca aagttctata cttaaattat
      121 caaataaaaa ggacgtgaag ataaatgtca aatttagaaa acaaagaaga aaaagttgta
      181 aacaaaattg tttctgttgt caataaactg gataaagagt tagacgaact tgatacgtta
      241 tctgaaaatc cagaaaaaaa acataaccta aaaaaatggt tagttgaacg aaaggctatt
      301 catgaaatta aaaaagttct tcatgaagct gataaatatg aaaaatatga tgaaaaagaa
      361 cttgataaag aatttaaaga aatcaatgac ttattactat aattgattta tacgactcat
      421 ttttgagtcg tcagactgca aacaacactc taaatttagg agtttgtcta cagtctgaat
      481 agattttaaa tttattgtcc ataatgttat tatgaattta tagcattaag aaaagagata
      541 agactttaca tttttttagg aatgtaaagt cttatctctt ttcttaattg tacaattagt
      601 attatctgca ctactttcta attaaataaa tttttagtta ttaacttgat taaaatcaga
      661 gataatatca tttaattcag tttgaagggt tgtaacgatt aaatggaaat tttcttcatc
      721 tgttttatta tatggggatg ttttcgttat atataacaac ttttcttttt caattttcat
      781 taataactct gtcgaagtct gtactttatc ttgataatta aatgcagaca taccactttg
      841 tataattgta attattgctg aagcaattga aacaatcaat agtaaagacc tgtgttcgga
      901 agcctgatca attaatattg ggatactagc tgataagact atttttataa tatttccgat
      961 accaattact ctattataaa atcgaatttc tttttttaat ctatcgattg tagaatcaat
     1021 agattcgata aacgcttttt gatccatcat tttctctcca atcattttat taaaatcatt
     1081 aacaaaaata ttataatagg atttaagtaa gttctaacta atcacatata aaaataaaaa
     1141 aacatttgta ttttatcaaa gttttattat acttgtcaat ttgtgtacat cctaaaatat
     1201 aagctatttg tttacttctg tagaatatat actttgttgt tagataagtt gcacatgttt
     1261 atagaatcta aataataaat ttttatgtac cgcccctcaa agtgaactga accccaaaag
     1321 ttgaactatt taatggactg tttccgatat tctactggag atagtccatt aaatagttca
     1381 acttttgggg ttcagttcaa agaggggggg attttttatc gttgtggaat atttaaatac
     1441 caacgcttgt catgaaaatc ttgtgctcca cccttagtat ttccttctgg atcgtttgtc
     1501 gcacgcatca tgacgtatac tttcttatta gggaaatcac gcatattgaa agatacatga
     1561 tagccaacat ttcctaaagt attataagct tgatttacgt ctggtctata aatcccatca
     1621 gctcttactc tagctagttc ttttccagta ttgtagtcca taatgaaaat atactcgtat
     1681 ttataattag caatgtgcca tccagctaca tgcaagtttg cgttttcgat ttctccgaac
     1741 tgatcaatgt gggcgtaatt tgttccatct gtcagcgtag aatttgcagc acctgctcga
     1801 gttggatcaa ttactggttt atcctccgaa gtagttggat tatcatccgt aaaaccatga
     1861 gccaaatcat aggctagttt ttctttactt acacccattt cagaaagata accgtaagga
     1921 tctgtatgat cgccccaaat attttgtgtt acccataaat gcgatttgat tcctggttgg
     1981 ttataaggag tgtctaatgt taatggaata ccatatttca ttgctgaatc tcttgccaat
     2041 tcaacgtata ctttatagtt cttttcaaaa gtttctttat catgtgtatg ttgtaactcg
     2101 atttgcacag gacttttggc tcacgaggtt gtcctactct acatcttgct gatagatatg
     2161 ctgaaaggaa aacaccaaca ttatgttttg ctttttcagc tagaacgaga caagcaggaa
     2221 tacctttaga agatatcaaa ggttttttag gtcataaaga tgtttccact acacaagtct
     2281 atgcccatat ttctcccgaa gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag
     2341 aagagcaaat aaaaaagcac tcaaattgag tgctttttta agctcactat cactaaaact
     2401 atcactaatt ttatttataa gcgaacatgt ttctatcact gattttttaa atctagcgaa
     2461 aaacccttat cttaaaggcg ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg
     2521 cctagaagtg cgaacatggt tttcttatag ctcatgtaga aaaattttct tgatgatgat
     2581 tttatccatc aattgagatt gactgttttg ctctcctatg atttcagcta tgattgtccg
     2641 attttgcggt tttcttctta actggtagtt gattttccca gtttccatca actcgctaag
     2701 catatccacg actccgccaa atccggtctt tttcgtaata aaacctagtg ctaccattcg
     2761 ttcatctaga ttttttgctg taatcgtcac accagtaatg agcgagtgct gtaatgtttg
     2821 ggccagctct tccatccctt cagcattgga tacgacggaa gctgttttgt taataggttc
     2881 ttcgtttatt tcttttggtt ctttcctctc cactttcact gtttgacgaa ggacatctag
     2941 ttcttgcaag agcgtttcgt attgttcttg ggtcagcatc acgccagcta ctttttcacg
     3001 attaaatata tatacgccag ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga
     3061 tcgtttcact tctgtgatcg aagatgtggg aacttctaat ttctttaatt ccaatggatt
     3121 tcctccttta ctaatcgctt tttattatag cacagactga cttgttttga tcttttttcc
     3181 aaaggtttta tcggtttttg cctaaccagt aattgatatc ggtaagaagt ctttcgatac
     3241 caaatgaagg atggtacaga tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat
     3301 gatcgatgat cactagatct gcataccgag gcggcttatt tcgagtacat acatgtatag
     3361 agatccgttt tcgatgtctt agcgttttgt tcagtacccc agcaagtctt cttgcttctt
     3421 ggtgttcata gcgaaaaaaa aagacgattt tcacttcagg agggtggaca tctaaatacc
     3481 ctcgttcctg gagtgttttc agtaaaagca tcccccactc tactgcgggg tgtgcaagtg
     3541 cttgttggat cgctagccga aattgatata gtttatcttt ggaattggga aatttttcta
     3601 atgtttcctg ataataacga acttcttcta tcagccggtc atggatacca attacgtaac
     3661 gaagtcattc ttggtaaagt acttttgtca gctttgattt gctttcgctc tcaaattcgc
     3721 tattcagcca aaacagcgaa tcaagctttt cagtcacaat cgtcaatttc cgatagtctt
     3781 gtgtctcttc tatccgtttc caccgctttt cagtaatctg gttacagctc aagcgcaaaa
     3841 ttgctagata atttttttct tttattggca aaatctgctc ctctctaaat agcaattcag
     3901 gaaacaattc aaagaatagt gttcgactgt ctcctaaaat ctcgatttct gccggatcct
     3961 ttctttgctt caataattgg atgaacatct cagaaaagga ctgctctcca tcatctgcaa
     4021 aaaaatgcgg aaaatctttc aaaaagcaaa acagcagata tcttactttt ctttctgaac
     4081 caaagatctg tgctttttta taagaaaaag aaaggtggta ctcgccaaac cactttctca
     4141 atcgctcttg cgtagaacga tcgtacttct gctcgtaagg ttcattcaat agcagatttt
     4201 tcagtaaaca aaacgggggt gattggatca gaaaaacaaa caagagataa ggttcgctat
     4261 atccttgatc ataaactaac tgaacggttt tcttatgaag aatcagctga atgggacagt
     4321 cgctttccgc caaacgtttc agatgccgat ccactgtttt aacatcgatt ttcagttctt
     4381 cagctaaatc agcttttcgc cagatcaaat ctttcattag attgattagt gaaaataaat
     4441 tctgtgcatt ttttgataat agtttttgcc atctcatcga tcatttctca cctgatttct
     4501 aaacatattg tttgatcaaa atgggttcac tcctaaaaag ataaaaaact ccctttctat
     4561 ttatttaaat acgcaaatct tcctcattat tttttatttt actcaattta atcgtattct
     4621 catttcaaaa cgaatgtact ttaaaaaaac ataataagat cactttgctt gtaggatgta
     4681 ttcccttcca ttacaatgaa ataaatagcg attttatgag aaggtgatgt gaaaatggaa
     4741 gaaaaaatag aagaatccaa acaaaaaatc gatcagttga tataagaatt agaaaaagcg
     4801 gtcatcggca aacgttcaac tatccaattg atgatcgtgg ccctattagc aggtggtcat
     4861 gtcttattcg aagatattcc cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa
     4921 gctgtacagg gaaccttcag cagagtccaa tgtactcctg atctgctgcc tagtgatatt
     4981 cttggttttt ccgtatacaa ttcacaatca aaagagtttg aatttcgtcc gggaccgatc
     5041 tttaccacga tcttgttagc agatgagatc aatcgtacga ctcctcgtac tcagtcagca
     5101 cttttagaag caatggccga gaatcatgca acgatcgaca acaatacata ccctctagac
     5161 aatcattttt ttgtcttagc tacgcaaaat ccaatcgagt acgaaggaac atatcctttg
     5221 ccggaagcac agctcgatcg ttttttattc cgactacaaa tcggttatcc ttcttttgat
     5281 gacgaacttt tgctactttt ggataaattc gaaaaagtgt tggataactt aaatgaagta
     5341 ttgaacagtt atgaaataga agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc
     5401 gaagtggcat cttatgcctt gcagttagtg tcagcaacaa gaagtcatgc tgccatccag
     5461 cttggcatca gtccacgagg aagcctttcc tttattcagg cagcaaaagc ttatgcttta
     5521 atccatggga gaaattacgt cacgcccaaa gatttgcagg atttagttcc ttacgtgttt
     5581 tcccatcgcc tgatttttta cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt
     5641 catactcttg ttgcacaaat tcctattcct gtgaggtaga ttgaggtaga caatggaaaa
     5701 aatcaaagat tatttcatta attttgtgtt atttctggta tatttcatca ttgtgctttt
     5761 tgctcttgta ttcaacaatt ctttaggctg gttcttgttg gcctttttta ctctttatgt
     5821 atcatggagc ttgatttcat taagcagctt tctaaagaaa ctttcggtta cttgtacatt
     5881 gcagccactc gtttatcgca aaaaaagtat gcaggttatt ttttccatag caaaaaagag
     5941 aactttttct ttacctctcc ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa
     6001 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     6061 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     6121 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     6181 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     6241 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     6301 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     6361 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa
     6421 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     6481 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     6541 ttggaagctg gcgatgagaa agcttttttc gcacacttga aaaaatatgc aaaaaaacaa
     6601 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     6661 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     6721 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaacggg aaaaatggct
     6781 gcgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     6841 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     6901 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     6961 gtttttatat tttccttcga agacattttc catattatgg atcttacagt ttttttcgtc
     7021 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     7081 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     7141 ctattggtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     7201 gaaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     7261 agcaaggcaa tttttctcca tccggttgtt gtcgggaaac actcgattcc agtcattgac
     7321 tacagttctg cttatttgtt tcttaacttt tagcgctatc ttgcctttat atcttccgaa
     7381 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     7441 gctttatgac acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     7501 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     7561 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     7621 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     7681 ctaccaaacc gttatagaag acaaagaaaa cattacattg accctaaaaa aacaagtttt
     7741 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     7801 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     7861 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     7921 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     7981 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     8041 cgaagctgtc taacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     8101 cgggcatact ccaaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     8161 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     8221 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     8281 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     8341 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     8401 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     8461 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     8521 accagcttat aaaatcgtct tattcagcct tggagtgtgt ttatccgtct tgttgttctt
     8581 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     8641 tgcttctact aatacgattg catatcaaaa acttcttaac cctttggaaa cattggtacc
     8701 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     8761 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     8821 agatccggta aaattcgata aaaaaaccta tttagaaata acgcatataa taagaaaaat
     8881 ggtatacaaa aaaattctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     8941 agaatctact tctggaatgc cgagaaccag ctgattgagt atcgattact cgaaaaaaag
     9001 gttgtgacac gttttttgtc acaacctttt ttcattatta tctgattttc aatgtcaaag
     9061 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     9121 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     9181 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     9241 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     9301 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     9361 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     9421 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     9481 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     9541 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     9601 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     9661 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     9721 tcacttgttg ttttttgact gttgcctcta gcccgactcc tgaaagattt tttagatttt
     9781 cagcttgata aggcttgatt tcatgttctt tcaaataatt cataatgcca acagccaatg
     9841 gatgattggc attcgcttcc aaggctccta gatattgaag gatttcattt ttattgaacg
     9901 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     9961 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
    10021 gtaatccgtt tttagctgct attgatgtgg aacgagcaac aactaatggg atagctaaac
    10081 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
    10141 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
    10201 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
    10261 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
    10321 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
    10381 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
    10441 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
    10501 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttc actgattctg
    10561 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
    10621 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
    10681 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
    10741 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
    10801 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
    10861 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
    10921 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaattcc
    10981 ccatgtgcat ggaatgatcc atgccgccgt gttcgtgatc catgtgcatc gaatggtcca
    11041 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tgatccatct
    11101 gactatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
    11161 ctacttcttt tttatgctca tgttttttct ccatatcgtt cccattcatt tttactccat
    11221 cccttctctt actttttcat gtgtcgattt aagcacaggg agttcaacaa tacactgacg
    11281 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
    11341 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
    11401 gtcaacctag acaactcgat tgtttgtgca atggatgcca aactgctatt catcagcgtc
    11461 acatcggctg tttccatcgc aatgtccgtt ccacttccca ttgccatacc gacatcagct
    11521 aatgctaagg ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
    11581 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
    11641 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
    11701 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt gacttctagt
    11761 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
    11821 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
    11881 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
    11941 ctgattccag cacctgggtg ggctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
    12001 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
    12061 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
    12121 gtaatcgttc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
    12181 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    12241 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    12301 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    12361 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    12421 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    12481 ccgacttgtg taacttccat gtgaagtaga ccattcgtat tgattgtccc gccaaagagc
    12541 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    12601 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    12661 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    12721 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    12781 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    12841 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    12901 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcgt cttgattgca
    12961 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    13021 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    13081 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    13141 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    13201 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    13261 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    13321 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    13381 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    13441 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    13501 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    13561 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    13621 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    13681 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    13741 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    13801 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    13861 gataataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    13921 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    13981 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    14041 aatcactgat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    14101 aatgtaatca ttatcttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    14161 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    14221 atcagtttat ttcttgcatt agctacttgg atcagctgct gtattttcgg ttcattgttt
    14281 tcttcaaaat gccagataaa agataaaaat tctaagtatt ccctgtttag tttctccgaa
    14341 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    14401 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    14461 ttaatccttt cctctaatgt agaagtataa tttccttcaa cgatccatga cggattagat
    14521 accataaaca attgctgttc ttgcaaaaac cacttttttg cctgaatact gtagtctgtt
    14581 gtatgccata aaaggtctag cggcagtagc ggaaagtcgg tgatttcttt gatcttcctt
    14641 gcgagggtcg attttcccga cccactttgt ccaatcacga tgatcttcat tcttctccct
    14701 cacttctctt tggaatttgg ttcgatcata ccacttataa cgccgcaaaa aaacaaggga
    14761 ctgtgacaaa agtcgtgtca aagtcccttg tttcgaataa
//
LOCUS       DAFCNS010000065        14197 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFCNS010000065 DAFCNS010000000
VERSION     DAFCNS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.067"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(12..482)
                     /locus_tag="LNE23_002097"
     CDS             complement(12..482)
                     /locus_tag="LNE23_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBK6931932.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="LNE23_002098"
     CDS             complement(641..1099)
                     /locus_tag="LNE23_002098"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBK6931933.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="LNE23_002099"
     CDS             complement(1270..2415)
                     /locus_tag="LNE23_002099"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBK6931934.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="LNE23_002100"
     CDS             complement(2428..3345)
                     /locus_tag="LNE23_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBK6931935.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="LNE23_002101"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="LNE23_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBK6931936.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4437..4607
                     /locus_tag="LNE23_002102"
     CDS             4437..4607
                     /locus_tag="LNE23_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931937.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="LNE23_002103"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="LNE23_002103"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBK6931938.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="LNE23_002104"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="LNE23_002104"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBK6931939.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="LNE23_002105"
     CDS             6871..7752
                     /locus_tag="LNE23_002105"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBK6931940.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="LNE23_002106"
     CDS             7749..8465
                     /locus_tag="LNE23_002106"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBK6931941.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="LNE23_002107"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="LNE23_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBK6931942.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="LNE23_002108"
     CDS             9182..10183
                     /locus_tag="LNE23_002108"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBK6931943.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="LNE23_002109"
     CDS             10180..11541
                     /locus_tag="LNE23_002109"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBK6931944.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="LNE23_002110"
     CDS             11501..12652
                     /locus_tag="LNE23_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBK6931945.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="LNE23_002111"
     CDS             12627..13796
                     /locus_tag="LNE23_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6931946.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..>14197
                     /locus_tag="LNE23_002112"
     CDS             13971..>14197
                     /locus_tag="LNE23_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6931947.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNV"
BASE COUNT         4590 a         2506 c         3022 g         4079 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttca
//
LOCUS       DAFCNS010000066        14119 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFCNS010000066 DAFCNS010000000
VERSION     DAFCNS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.068"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..556)
                     /locus_tag="LNE23_002113"
     CDS             complement(<1..556)
                     /locus_tag="LNE23_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBK6931948.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="LNE23_002114"
     CDS             complement(572..1051)
                     /locus_tag="LNE23_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBK6931949.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="LNE23_002115"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="LNE23_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="LNE23_002116"
     CDS             complement(2098..3204)
                     /locus_tag="LNE23_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBK6931950.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="LNE23_002117"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="LNE23_002117"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBK6931951.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6336..6716)
                     /gene="yajC"
                     /locus_tag="LNE23_002118"
     CDS             complement(6336..6716)
                     /gene="yajC"
                     /locus_tag="LNE23_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBK6931952.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6813..7958)
                     /gene="tgt"
                     /locus_tag="LNE23_002119"
     CDS             complement(6813..7958)
                     /gene="tgt"
                     /locus_tag="LNE23_002119"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBK6931953.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8176..8874
                     /locus_tag="LNE23_002120"
     CDS             8176..8874
                     /locus_tag="LNE23_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK6931954.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9042..11681)
                     /locus_tag="LNE23_002121"
     CDS             complement(9042..11681)
                     /locus_tag="LNE23_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6931955.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12300..13331)
                     /gene="queA"
                     /locus_tag="LNE23_002122"
     CDS             complement(12300..13331)
                     /gene="queA"
                     /locus_tag="LNE23_002122"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBK6931956.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13524..>14119
                     /locus_tag="LNE23_002123"
     CDS             13524..>14119
                     /locus_tag="LNE23_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6931957.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3959 a         3096 c         2543 g         4521 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac actttttttg
     6121 tgattttttt cacagttatt tttaaatata aaattgcttt atttacaggt gtttgtatag
     6181 aaaaatatat aaagttattt attattattt atatgacaca cccgattatt caatattgag
     6241 caatatatcc catgctctta tctctttctt ttttgaataa aaaatgaggt aggacatctt
     6301 tctgccctac ctcgtcttta aataagcttt gatacttatt tttcttcctt ctcttctgtc
     6361 tcttccagcg tttctgttgt ttcagttttt acttcaactg tctcagctgg ttcttctgtt
     6421 gtttcaacag tagtcgttgc atcatttgtt acgacagtcc ctggtttgac cgttttgatt
     6481 gctgcacgat cgtactcaag gaagatccct tcgcagtcta aaacaactgt ccgtttgtca
     6541 ttgtcgattt ctgaaacaac tccatgtaat ccgccgatcg ttacgactga atcacctggt
     6601 ttcatgctat ctaacaagtt ttgacgttct tgctgttgtt tcttttgcga acgtgacata
     6661 aaaaaccaca ttgctaatag cgcgataaac atcacaagca ttggtaatgt tcccattttt
     6721 ttcacctcac taattctgta tctacaaaaa acactttagc agaaaaatgc tgataacact
     6781 agttttttac ttaattttta aaagattttc ctttagaagc tttttgcgtt ttctttattg
     6841 aacccgtatt cttcaaaaaa ggcttctcta aattcaagca aattatcatc catgattgct
     6901 tgacgtactt gtttcattag attcaacaag aagtataaat tgtggtaaga agtcaaacga
     6961 atcccaaatg tttcatcaca tttgatcaaa tggcggatat aggcacgtgt atagttttta
     7021 catgtataac aatcacattt ctcatcaatt ggacggaaat catgggcata tttcgcattt
     7081 ttcactacta gacggccttg agaagtcata catgtgccat ttcgagcgat tcgagtaggc
     7141 aagacacagt caaacatgtc tactccacgg ataacaccat cgatcaatga atcagcagca
     7201 ccgacaccca tcaagtaacg tggtttgttt tcggggatca acggtgtagt gaattctaat
     7261 acacgattca tttctgattt aggttccccg acagataacc cgccgattga gtaaccaggg
     7321 aaatccatcc cgactagatc tttcgcgctt tgacgacgaa gatcttcaaa ccctgctcct
     7381 tggatgattc caaataatcc ttgacggtct ggattagcat gagctttcaa tcctcgttct
     7441 gcccatcgag acgtacgttc tactgatttt ttcacgtagt cgtaactttc atcgaatggt
     7501 ggacattcgt caaaactcat catgatatca gagcccaatt tattttggat gttgattgct
     7561 ttttctggtg ataaaaacat acgcgaaccg ttcaagtgat ttttgaaatg cacgccttct
     7621 tcttcgatat tccgcatatc acttaatgaa aaaacttgaa agccgcctga atctgttaaa
     7681 atcggttgat cccagttcat aaatttatgc agtccgccag cttcttctac taagtcttca
     7741 ccagggcgta gccataaatg atatgtatta cttaaaataa cgccagcacc catttctttc
     7801 aactcttctg gggacattgt ttttactgta gctaatgttc ctactggcat gaacattggt
     7861 gtggggaaag ttccgtgtgg cgtgatcaat tcccctaaac gcgcgccagt atgtttttct
     7921 ttcttgatta aacgatagcg aatggcaggt tgcgtcattc atttccctac tttctatata
     7981 aaatatcttg acaactccca ccatcataat cgtttcaagt gctttttgca agttccctcg
     8041 gcttccttct actttattta atattctttt ttttatcctt tttttatgaa taacaaaaaa
     8101 aagaaaaaca ttatgacaga aatatttttt gatacatggt atattggtgt tattaaagaa
     8161 aaggagggaa aaatcatgca atcttctgca gaattaaaac gccaagcaaa agagagtcta
     8221 aaaggacgtt ggggacaagc tgttttacta aatctaatcc ctacattgat tggtattgct
     8281 actattcttc ttattatgat tcctattatc ttgtttgcca ccaccatata tgatggatca
     8341 gcgagcgtct cagaagcgac aacttcagga ggcggtagtt caggaggcgg tggcggaatt
     8401 atttccagca tcattagcgc tttgttcatg agcggtattt cttggactta tcttgatatt
     8461 ttacgtggac aaaaagatca gatcgaacca ttcaaagatg catttcgtgg attcaaaggt
     8521 ttgtttatcg gcggagtgat cagcttagct gtgctgatct cgatctttac aacattatgg
     8581 ggtttgctat tagttattcc tggaatcatt aaaagctatt cttattctca aagctatttt
     8641 atttactacg atatcgtcca agaaacagga caaacaccta aagtactgga tacgatcact
     8701 gcaagtcgaa aattgatgga tggttataaa ggacaacttt tctggttaga tctttcattt
     8761 atcggctggc atatcttagc attagcaact gctggtattg gttatctatg gctcagccca
     8821 tacatttctg caacaaaagc agcattttat gaacaattgc caaaagatat ttaaaagaga
     8881 gtgtgacttg tgaccgaggg ggtggcagga gaacaccgtt tatcgagagg cagtaaaaga
     8941 aacattcagc ccattagcaa aaaggaggtg tgacaacatt catgtcacac ctcctttttg
     9001 tatagttcca gtaaaaagaa aatcaactta gaaacagtta atcattcctc ttcttctgct
     9061 tccggtaatc gatggaactt gatcagttca ttcaccgcga ttggtacgat agataaaatc
     9121 cctaccaaga accagtgatt caaactgatg tgtactaggc cgaatagttc ttgcgtaaat
     9181 ggcagcaaag caacagccgt tgtaatagct aatgccagta acgccatttc taccaaagct
     9241 ttattggtcg ctaaattgac tttgaagatc gattggctgc tgcgtacatt gaacacatgc
     9301 agaatcgatg aataagctaa gatcaagaat gcaacagttt gaccgacatg ctgacttgcg
     9361 tctacccatg ggctgatagt atcgacataa ctacctaaat aataaccaaa caaggttacg
     9421 atggtgaaga cccctgcatt tatggcaatt ttttgcaata aacctcgagc aaagatccct
     9481 tcatttttcg gaatgggtgg ctgatccatg atgccgtgtt ccgctggctc tcttcctaaa
     9541 gcaaatcctg gaagtccatc agcaactaca ttgacaaaca gtagttgcac agctgtaaat
     9601 ggtgctcccc atcctaacag catcgcaatc aaaacaataa agatctccga aatattacag
     9661 cttagcaaaa agttgatagc tttacgaata ttctgataaa ccgaacgtcc ttgactgaca
     9721 gcgtctacga tcgtcgcaaa gttgtcatcc gtcaaaatca tatctgcggc actttgcgcc
     9781 acatctgttc ctgtgatccc catggcacac ccaacgttac ttgctttcaa cgctggtgca
     9841 tcattcacac catccccagt catcgctacg actgcacccg tacgttgcca agatttgacg
     9901 atccggatct tgtcttctgg cgttacccga gcatagacag agagagcttt gacacgcgca
     9961 tctaattctt cgtctgataa ttgatgcagt tctgatcctg aaagtgcttc tgacggatca
    10021 ttcaagatac ctaattcttt cgctatggca ctggcagtca ccacatgatc tccagtgatc
    10081 atgactgttt tgatgccagc ctttttcgct cgcttgattg ctcctttact ttctggacgt
    10141 ggtggatcga tcattccgat cagaccaatc aaacgtaagt ctttttccag tgcttcagaa
    10201 gaaatatctt gcggttcttc gtcataagta gcataaccaa cagcaatcac tctcagtgcc
    10261 cgtttaccaa agcgatcatt aacgatcgcc gcttgttcta catcgcctga actaaagcgc
    10321 ggagcaagaa catcgaatgc tcctttggtg attgagatgt atttttcgcc catttggtga
    10381 actgttgtca tcatttttcg ttcagaatca aaaggaagct cgtttactcg tggatatttt
    10441 tcttctaatt ctgcctttgt atgataattc tcttcaacag cccttacgat ggctacttct
    10501 gttggattac ctttgataac aaggtcgtca tcttcttgct cgaccgtcac atctgtacat
    10561 agtgcagcca ttttcaatat ttccatcgcg ctgttcgtca ttgaatcttc gatattcgtt
    10621 acttcatctt ctttgctcca tacccggcgg acgcgcattt cattttgtgt cagcgttcct
    10681 gttttgtctg agcagataac attagcggtt cctagcgttt caactgctgg caactgtcgg
    10741 atgatcgcat gtttcctcgc catcttctgc acaccgtatg caagtgtcaa cgtaacaata
    10801 acagtcaagg tttccggtac agctgcaaca gccaaggaaa tcgaagtcat gaacatctca
    10861 aggagtggtt ctccttgcag ttcaccaatc acaaatacta aagccgcagc cgctaaagcg
    10921 atcatactga ttcgtttgcc tagctgattc aatcggacct gtaaaggtgt tttttgcata
    10981 gtattttcgt tcaaaagatc cgcgatctta cccatttctg tagccatgcc aatggcagtg
    11041 acgatcgctt tcccacgacc tgcagcaacc gtacatcctt taaataccat attaagttgg
    11101 tcacctaatg actcatcttc ttcagcaata aatgctgcat ctttttcaac tgcttcactt
    11161 tctccagtca acgccgattc ttctacacgt aattgtgatg cttcgatcaa acgggcatcg
    11221 gcagaaatag catcaccgga ctctaaaaca agaatatcac ccactaccag ttgctctgac
    11281 tcaacagtgg tcactttgcc gtctcgtatg acagttgccg tttgtttatt catatcttgc
    11341 aaggattcca gtgctttttc tgcgtttcct tcttggacaa tcgccaatac cgagttgatc
    11401 acaacgatag cgatgatcag cagtccttca aataaatccc catgttctgt agcaaatgcg
    11461 gtataaaagg aaatcaccgc tgctacaaga aggatgatcg ttgtaaaatc ggacaagctt
    11521 cgaaagaatt ttttgatcat cgattctttc ggcgcctcat cgaatttatt caatccctgc
    11581 ttttttcgcc gttcatccac ttcttgtgaa gacaacccag tatctggatc tgcttctgtt
    11641 accttcttta cttcttctat tgtctgcttg tattcttcca tcctattcct cctttaaaat
    11701 cttcgtacaa aaaaaggtac ttattttact ataacatcct ttttttattt acttcaaata
    11761 aagggcttat cgattatttg ttaagaaagc gtaaaattta aagtgcttat atttgcaaac
    11821 gctaccaaag gatgctatta tatagatgta aggaaggagg ataccatcaa atagacagaa
    11881 gaatttctcg atagaacagt gtaagcaaac aaagttgtta gaagtacagg tttcacccag
    11941 acatgcgtgt agagtacaaa cctataaata gtgaatgcgt atggatgttt ggatcacgaa
    12001 gtatccgaaa caagaagtaa tgcgatacgt aacaattgaa taacgtaaac gtctgaaaat
    12061 tgaataggaa caagagatgt ttcaaatgtt gaagatgtaa cgaaaacaaa atttttgttt
    12121 aacatgaaga aattcaacac cgtgctttgc atgtatgatg gaaagttaca atcatttctg
    12181 tttatttgaa tccttccgag gtacaagaaa gccgagctta tcgagagctc ggctttttct
    12241 ttgtcataaa atctttgtaa agcttttttg ttattcaaaa ctagcggctt ttcataacct
    12301 tatttcacga acatcgcatc tccaaaacta aagaaacgat agcgctcttc aatcgcatgc
    12361 tggtaagcag ataaagtcaa ttctcttcct gcaaaggcac taactaacat gactaaagtc
    12421 gattttggca aatggaaatt agttgagaaa gcttctacca ctttgaattc atagccaggc
    12481 gtaatgaaaa tatcagtcca tccgctgtct gctttgattt ctccattaaa tttcgttcca
    12541 atcgtttcta atgttcgaat tgaagttgtt ccgacagcca cgattttccc gccattttta
    12601 cgtacttggt tcaatcgctc agccgcttct tccgtcaaac ggtagaattc actatgcata
    12661 tggtgttctt caattttatc tacgctgaca ggtcggaatg tacctaaccc tacatgtagt
    12721 gtcagataga ctaattcaac accctttgct tcgatctcat caagtaattc tttcgtaaaa
    12781 tgcaatccag ctgttggcgc tgcagcagaa ccattttctt tcgcatagac agtttgatag
    12841 cgatctgggt cttccagacg ttctttgata tatggaggca atggcatttc tcccaatgat
    12901 tcaagaatct ctagaaaaat tccttcatac tcaaagtcaa tgatccgtcc gccgtgttcc
    12961 aattcttctt tcactactgc ttgcaatctg ccatcaccaa aagagattct tgtcccaact
    13021 ttggcacgtt tcgcaggttt gatcaatgtc tcccacgtat ctccttgtgt attcgttaga
    13081 agaaggactt ctaaatgcgc accagtatct ggtttttctc catataaacg agcaggcaga
    13141 actcgtgtat tattcataac tagcgcatca ccaggctgta actcttcaat gatttcatgg
    13201 aaatgtttat cttccatctc tccagttttc cgatccaaaa caagtaaacg agaatgatcg
    13261 cggtctttta atggtgtttg tgcgattaat tcttccggca agtcaaaatc aaaatcttct
    13321 gttgttaaca tatcgtcact cactttctat catgtgcttc atcattctat catgtttttc
    13381 gttctgttgg caaaatcagc actttttcac ttctattttt tgttttccta aataaaaaaa
    13441 ctaccctatt tgtatttttt cattgtttta ggcatactag taatagatag ttcttcattg
    13501 aaaatagaaa aagaggtggc accatgattg aatttcaaaa tgtgtcaaaa atttataaag
    13561 gtggaaaagt cgctgtagag gacgtcaatc tttcatttga aaagggtgag ttcatttgtt
    13621 ttatcggtac gagcggaagc ggaaaaacga cgacgatgcg gatgatcaac cgaatgactg
    13681 atccaacaca agggaaaatt ttgattgatg gaaaatccat ccaagaaatt gatccagtgg
    13741 aattgcgacg tcagatcggc tatgttatcc aaagtattgg attgatgcct cacatgacga
    13801 tccgtgaaaa tatcactctt gtccaaaaat tgttgaaagt cagtcaagaa gaacggaata
    13861 aaacagctga aaaaatgatt gatcttgtgg aattgccccg tgagatgtta gatcgctacc
    13921 cacatgaatt atctggtggt cagcagcagc gtattggagt cgttcgtgcc cttgcagcga
    13981 atcaagatat catcttgatg gacgagccat tcggtgcgct tgatccaatc actcgtgatt
    14041 ccttgcaaga ccttgtcaaa gatttacaag agcgtttggg aaaaacgatc gtatttgtaa
    14101 cccatgacat ggatgaagc
//
LOCUS       DAFCNS010000067        14107 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFCNS010000067 DAFCNS010000000
VERSION     DAFCNS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.069"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            262..2427
                     /gene="acm"
                     /locus_tag="LNE23_002124"
     CDS             262..2427
                     /gene="acm"
                     /locus_tag="LNE23_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBK6931958.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2699..3184)
                     /locus_tag="LNE23_002125"
     CDS             complement(2699..3184)
                     /locus_tag="LNE23_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBK6931959.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3274..3619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3664..4305
                     /locus_tag="LNE23_002126"
     CDS             3664..4305
                     /locus_tag="LNE23_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6931960.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4324..4959
                     /locus_tag="LNE23_002127"
     CDS             4324..4959
                     /locus_tag="LNE23_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6931961.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4977..5792
                     /locus_tag="LNE23_002128"
     CDS             4977..5792
                     /locus_tag="LNE23_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6931962.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6111..7547
                     /locus_tag="LNE23_002129"
     CDS             6111..7547
                     /locus_tag="LNE23_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6931963.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7736..10183
                     /locus_tag="LNE23_002130"
     CDS             7736..10183
                     /locus_tag="LNE23_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK6931964.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10413..11480
                     /gene="rlmN"
                     /locus_tag="LNE23_002131"
     CDS             10413..11480
                     /gene="rlmN"
                     /locus_tag="LNE23_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBK6931965.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11673..12776)
                     /locus_tag="LNE23_002132"
     CDS             complement(11673..12776)
                     /locus_tag="LNE23_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6931966.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13329..13877
                     /gene="aac(6')"
                     /locus_tag="LNE23_002133"
     CDS             13329..13877
                     /gene="aac(6')"
                     /locus_tag="LNE23_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK6931967.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4851 a         2320 c         2980 g         3956 t
ORIGIN      
        1 agttaaaaat tggaaatata gattgaaaaa atgaaagcaa taagtaaaat aaaatgaata
       61 tatgcgaaaa aataatttac attaggaaaa accttgtaaa aacagttttt tggcatattt
      121 attttttata aatatttgta aaataactat caatttttta gttaaaagat tggaaatgag
      181 tgggggagga attgaagaaa ggatttttga gagatgatat agtaggtttt atttttagtt
      241 aaaatagggg agatagaaaa aatgaaaaag tgtagaaata ttattttatc aatgttattt
      301 attattacta atataacatc actgattcct gttcatgtct atgcggatgc aggcagagat
      361 atcagcagta atgtcacttc gttgaccgtg gacccaacga atattacaga tggaggaaac
      421 ataaaagtaa aattctcgtt tgacgaaaaa aaacaaaata tccaaccagg tgattatctt
      481 tggatcaatt ggccaagtga aggaaatatt cgcggtgaag gattccaaaa agaaatccca
      541 ttaatgattg aaaataaaaa tgttggaact ttaacggtca gaaaagattc tgcgcaggtt
      601 gtgtttaatg aaaatattaa aaacctagac tcagtagaag gttgggggca atttgagata
      661 caggccagaa acgtaaccga taccggtgaa gaaaatacgg gaccattcac cgtgacgagc
      721 ggtgataaaa cagctactgt aaatgtgaca aagccagctt ctggttcttc ttctagcgtt
      781 ttttattata aaacaggaga catgctacct gaagatacta agcacattcg atggtttttg
      841 aatattaaca ataacggcac ttatgttgaa caaccagtta aaatcagtga tgaaatccaa
      901 agtggacaaa gattagatcc aagtacgttt gaaattaatc aaatacactt aggtgaacag
      961 aaggtttata gaggagaaga gggaatccaa caatttttac aagatttccc cagcgctaca
     1021 tttaattttt cagtaactga taattacatt gaaataacta taccaaaaaa ctttgtcaat
     1081 cttaggaaaa taatggtgag ttataagacg atcatagaaa acccagaaca aataaatttt
     1141 gagaatcatt cggaagcttg gtttaaagaa tttaataaac ctgctgttga tggagaaagt
     1201 ttcaatcata cagttaaaaa tatttcagct tctggtggag tcaacggtac agttagaggc
     1261 gaattgaaga ttttcaaata tatcaatgac acagagattg gtatcccaaa tgtgactttc
     1321 gaattaagac gagcagatga acagcctatt caaggacagt ctagtatcct gttgacttcg
     1381 aatgaacagg gagaagctag tatcaaaggc ctgcaagtag gagactatgt tgtaaaagaa
     1441 aaagaagcac ccaattggat tgattttgat ccactaagtt caaatgaact caaattctcc
     1501 ataaacgaaa atgatacaga gggagtcagt ttacctattt ataacaagaa aaaggttacg
     1561 aacatcacag caactaaaat ttggaacggt ggaaccactc caagaccgag tatttatttt
     1621 aaattattca gagcaagtac aaacaattgg gaaccagttc ctgatgcaga aacaaaacga
     1681 ttagacaatg gaataacttc agtgacttgg gaagatatcc aacagtatga tgatagtgga
     1741 aacgaatata cgtttaaagt gcaagaagta gatcaaaacg gaaatgatta tgtcccttca
     1801 ggttatcaaa aaattgaaaa cgggctagtc gttacaaatg agaataaaga agtcatttca
     1861 gtctcaggac aaaaaacatg ggaagacaat gagaaccaag acggcaaacg accgacgacg
     1921 atcacagtaa atctattagc cgacggtcaa ccgatccaac acaaagaagt aagtgaaaaa
     1981 gatgattggc gctatcgttt cacaaatctg ccaaaataca gagacgggca agaaatcatc
     2041 tatactgtaa cggaagacaa cgtgcccgaa tacagtacga cgattgacgg gtataacatc
     2101 aaaaacagtt atacaccgga aaaaacaaat atatctatac caggtagcca tataacaccg
     2161 tggaaaaatt ttccagagaa gacagagaga gagaaaatta gaaatctatt ctctcaaaaa
     2221 ttgcaaatat ttgctcatac aggagaatcc agcgaacaaa gaagcaatta tattagtaag
     2281 agagctgtac ctaaacagat aacaaataaa tcaaaaagca ttcttcctaa aacaagtagt
     2341 aaaaaatcta gttttgctgt aatcattggt ttattgatag taactatctg tgctgggata
     2401 ttcttacaaa aatataaaaa agtttagtta gaatataata ttttttgtta aatatgactg
     2461 aaatcaaaag aataaaattc cgatttataa tcgaaaaaag aagcattttt atatatgctt
     2521 cttttttcgt agaatcaata aatatggatt tggctaacta tggaagaata ctcgtattag
     2581 ttttcagcgt tcatactagt tgttacattg tttgattggc caaaagaatt tctgatatat
     2641 tttgtgctaa ataaagaaaa agacgcttga attgggaaat aagaaaacta ctctatcatt
     2701 acaaattttc ataaactggc agatgttcta agtaaacaag tatcttttca aagtcggaaa
     2761 aatccataat caacgtagtg gtattgatat ttggatgaaa gcctacggta tcttgacgat
     2821 caatagcagc atcgatcacg acttggattt ttttctcttt gtcgtgcatc aatgctaatg
     2881 gagtcactgt acctgctgga acgcctagga ggtcaggaag ctgttcttca gaaacaaaag
     2941 ataagcgctt ttcttttaat tgttcagcta gttgttttaa atcggcgtgc ttttcatcag
     3001 gcaagatgac aagatagatt tgttttcctt ttcttgattt caataataga ttttttactt
     3061 gcggaccagg tagggaaaaa gggacatttt tgactgacgt aatcgcagga tgatcgactt
     3121 tttgaaaaga gatacccagt ttttttagta gttcataagc ttcttgttcg gttgcacgac
     3181 tcatcgcgca tcaccctcct ttttttatta gtgtatcata aaaaagagtt gacaaatgaa
     3241 aaataatcat gtaaattaca cgtaaagtta aaacaaatga catcgaaaag aagagtagcc
     3301 gttaggtgtt tttagagagt ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca
     3361 catcttggag tcgattttct gaagcgtata gtaggaaaat ccggggagcc cgttacagct
     3421 gcggtcgttg ctagatgacc agataaggct ggcttttccg tgaggagtcg gtaaaaaaag
     3481 gtggaaccac gtgattaacg tcctttgatc ttcggatcaa aggacttttt gttttcatct
     3541 gaacggcaac gactgtgtga ggttttttct gcaaggaaaa aataaacaga ggtggaacca
     3601 cgtataatac gtcctcttgg catttgccaa gaggattttt ttattttaaa ggaggaaaag
     3661 aagatggagt atttgattga agttatgccg caattattag taggagcact cacaacattg
     3721 aaattatttt tatttacctt gattggctca ttaccattag ggattgtttt agcattagga
     3781 ttgtctttgc attttaagcc gctacgctac ttgcttcaaa catatgtatg ggttatgcgg
     3841 ggaacaccgc tgttgttgca actgattttt attttttatg ggttgccgac gattgggatc
     3901 gtttttgatc gtttcgaagc agcactcatt gcctttatct taaactatgc agcttatttt
     3961 gcagagatct ttcgtggagg gatacaatcg attcctcaag gtcaatatga agcagcgcaa
     4021 gttttgcaat taagtccctt tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag
     4081 attgttttac cttctgtagg gaatgaagtt atcaacttgg tcaaagattc ttccttgatc
     4141 tatatattag gtttaggtga cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg
     4201 actttactgc ctttgctagg cgtggcaatc atctatctta cattgactgg tattctaaca
     4261 atcgtaatga aacaagttga aggaaaatta aattattacc gctaactgat aggaggaaga
     4321 aatatgattg aattgaagaa agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat
     4381 ttgaatctaa tcattccaga tgggaaaaca ttagctatcg taggtccttc cggtggcggt
     4441 aaaaccacgc ttttacgtat cttggctggc ttagaaagtt ctgacagcgg agaattttat
     4501 ttagacggtg taccgttcaa cccattcgag cttaaaatga aagaacaagt tgtaggagtc
     4561 gtattccaag acttccagct atttccacat ctgaatattt ttgaaaacat taccttagca
     4621 ccaaaaatgg tattgaaaca gacaaaagaa gaatacacca aaaaagcaga agaattagcg
     4681 gcttcattag ggattagtga tttattgaat atctatcctt atcagctttc tggcggacaa
     4741 aaacagcggg tagcgattgc cagagcattg gctatgaatc cacgggtatt ggcttatgat
     4801 gaaccaacaa gtgctttaga tcctgaatta cgccagcaag tcgagcgttt gatcctatca
     4861 ttgaaagaag atggggtaac ccaaattatt gtcacccatg atttagtatt tgcagaaaat
     4921 gtcggtgatc aattactaaa agtttcccca attcattaag aaaggagaag atagagatga
     4981 aaaaaaccag ttttctattc gctttgatcg caggtcttct tctttttgca ggctgttcga
     5041 acaaaaaaac ttcagcagat aattgggcga ctatcaacga agagaaaaaa atcgttatag
     5101 gtttagacga tacatttgtt cctatgggat ttagagataa agacgggaag ctcacaggat
     5161 ttgatattga tttagccaaa gcagtctttg aacaatatgg gattacggta gactttcagc
     5221 ccattgactg gtcaatgaag gaatttgaac tgaacaatgg aacgatcgat ttgatatgga
     5281 acggctattc caaaacgccg gcaagagaaa agaaagtgca atttacaaaa ccttatatgg
     5341 aaaacgatca agtgctgatc acacctaaag aatcaggaat cacaaacttt ggacaaatga
     5401 ataataagag attaggtgcg caaaatgggt catctggtta tgatgtattt acgaaacaac
     5461 cagaagtatt aaaagagata gtcaaaaacc aagatgctgt tttgtatgat agttttaatg
     5521 aagcattgat cgatctgaaa agcagacggg tcgatggtct gctgatggat aaagtatacg
     5581 cagactatta tttgaaacaa agaaacgaac tagaagactt caacataaca aaaggttctt
     5641 atgagagtga ggattttgca gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa
     5701 tcaatgaagc cctagatcaa ctagagaaaa caggtgaatt ccaaaaaata tccgataaat
     5761 ggtttggcga agatgttacg ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg
     5821 tcacagtccc tttttccgaa taaacagtgt tctcctgcca actccttgtg actcggagct
     5881 gaatggcagg ctcacaacct cttggtaaac ggtgttcaaa agccagtttt tcggtattaa
     5941 gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa atttttctta ctactcgaaa
     6001 ctaagcgact ttttcacaac ctcctttaca tatttttatt ttgttgtgga aggggtttcg
     6061 tgttattcta aaagaggaac gattgaaggt tataggaaag gaagagatga atggaacaca
     6121 aagaacttac ctcatttcca aaagattttc tgtggggctc tgcttctgca gcgtatcaag
     6181 tagaaggagc ttggcaagaa gatgggaaag gagaatcagt ctgggatcga tttgtacgga
     6241 tacctggaaa gacatttaaa ggaacgaacg gtgatttggc agtagaccac tatcatcgtt
     6301 ataaagaaga tattgcttta atgaaagaac aaggtctgaa agcttaccgc ttctctgtag
     6361 cttggacacg gatttttcct aatggacgag gagaagtcaa tcaagctggg ctcgcttttt
     6421 atgagagatt gatcgacgaa ttgattgaaa acgatattga acctatgttg actttatatc
     6481 attgggatct tccacaagca ttgcaagatg aatataatgg ttgggaatca cgtcagatca
     6541 ttgaagattt cactcattat gcagaaacat tatttgaggc attccgcggg aaagtccatt
     6601 actggattag tttgaatgaa caaaatatct tcacttcatt aggttatttg ttagcagcac
     6661 atccgccagg tgtgacagac ccgaaacgaa tgtatgaagt taaccatatt gctaatttag
     6721 caaatgcatc tgtcatcaat aaatttcacg agttagaaat gcctggaaaa atcggaccaa
     6781 gctttgcgta tacgccaaat tatccaattg acagtaatcc tgaaaatgtt ctggctgctg
     6841 aaaatgcaga agacctgatg gccaattatt ggatggatgt ctatatgtgg ggaaaatatc
     6901 cgattgctgc catgaggttc ttagaagaaa aaggatgggc accaacgatt gaagctggcg
     6961 atgcagaatt gctagaatct gctaaaccag attttcttgg gatcaattac tatcaaacgg
     7021 cgacgaatgc attcaatcca ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa
     7081 aaaaaggttc atcagaagaa actggagttc ctggaatgta taaaaaagtt gagaatccat
     7141 ttgtcgaacg aacaaattgg gactgggaga ttgacccaga aggattaagg atcggactgc
     7201 gtcgtatcac aagccgttac cgcatgccgg tactgattac ggaaaatggc ttaggagagt
     7261 atgacaagtt gacagaagat aaacaaatcc atgacgacta tcgaatcaat tacttacaaa
     7321 gccacgtcaa agccatcaaa gaagcaattt cggatggagc agaagtccta ggctattgca
     7381 cctggtctta tacagatttg cttagctggc tgaacggtta tcaaaagcga tatggatttg
     7441 tatatgttga tcaagacgaa acacaagaag gatctttgga acgatataag aaagacagtt
     7501 attactggta ccaaaaactg attgaagaaa atggagagaa cctttagttc acatacttaa
     7561 aacaagaagg tacggctgga tatggccgtg ccttttcttg ataaaatcat ttagaaatta
     7621 agaaaaactg taaaataagc tgaaaaatga aagaaagcgt cagttctttc aaacaaaact
     7681 ttagttccct tacttttttt tgtataatga aacttgatga aatgagggat aataaatggc
     7741 acaaaagaaa cggcctacta aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca
     7801 gcagcaggaa caaatgctga cgattggtgt tgggtttgcc ttgattttat ttgctgtctt
     7861 cggcttttta aaattaggtt ttttaggaat attgattgca aacggctttc ggatcattgc
     7921 aggaaatact taccaaatcc tctgtctttt attagccgta ttaggatttt ggattgtcat
     7981 taagaataca gagttttcaa ttggtaaaaa ccgacgctgg tttggcggca tactctttta
     8041 tttcggtatc ttattattgt tacatgccca tttatttggc aaactccata ctggggaacc
     8101 aaatatcatg gggacgacat gggatttgtt agccagtgat ataaaacaaa gtcaagtaga
     8161 caataacgtt ggtggtggga tgattggagc aatcctttat cattttactt acttcttgat
     8221 tgcacagcct ggaagttatt tagcagcagt gctattactg gcaggaggag cattcctgtt
     8281 cagtaatctg gaaggctatc agctgttaaa tggtatccaa tcaattggag aacgagtaca
     8341 ggaattattg gaaggtgatc ctgccaagca agctcgaaag caagcggcaa aggaagagag
     8401 aatgaaacag cgcgcagaag cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga
     8461 aaaagaagct gcagaatatg agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc
     8521 tgatgaatgg caggaaccag aaaatacaga gccggagcag ctaagttttg ttccaatcga
     8581 cagttttcaa gaaaatatcc atcctgcgaa tctagagaaa ccagtaccag atacaccaaa
     8641 acagaccaat acagcagaag gtttttcaga tgagttgcca gaggatgacg gtacctcatt
     8701 agaatttgag attgaagcag aacaggaaaa tcaagactat gaattaccga cagttgattt
     8761 gcttgattct ataccaactg tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat
     8821 cggcgtatta gaacaaactt tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag
     8881 cttaggacca tccgtcacaa aattcgaagt ccagccggct gtaggggtaa aagtaagtaa
     8941 aattgtgaac ttaacagatg atatcgcgct tgctttagcg gcgaaagatg tccggatgga
     9001 agcaccgatt ccaggtaaat cactgattgg tattgaagta ccaaatggaa agatcagtat
     9061 ggtttccttt agagaaatca tcgaagcaca accaaaccac ccagataaat tactagaagt
     9121 acctttagga cgtgatgttt ctggacgggt acaaacggct gatctttcga agatgcctca
     9181 cttactcgtg gctggttcga ctggtagtgg gaagtcagta gcaattaatg ggattatcac
     9241 aagtatatta atgcgtgcta agccccatga agtaaaactg atgatgatcg atccgaaaat
     9301 ggttgaattg aatatgtaca acggcatacc tcatctgttg acgccggtcg tgacgaaccc
     9361 tagaaaagca gcgcaagcct tacaaaaagt agtacaagaa atggaagaac gatatgaaaa
     9421 attcgcggca accggagtaa gaaatatttc tggttacaat gaatttgttc aacagaaaaa
     9481 cttggaaaac ggaacgaaac atccaacctt accattcatc gtagtgatcg tagatgaatt
     9541 agctgatttg atgatggtag ccagcaatga agttgaagat gcgattattc gtttagcgca
     9601 aatggctcgg gcagcgggga ttcatatgat cctagctact caacgtccaa gtgtggatgt
     9661 tatcacagga atcatcaaag caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg
     9721 tacagattct cggacgatca ttgattctaa tggggcagaa aagctcttag ggcgaggaga
     9781 tatgctattc ttgccaatgg gtgagaataa accgatccgt gtgcaaggag cattcatttc
     9841 tgaccacgaa gtagaacggg tcgttcaatt tgttacagat cagcaagaag ctcattatga
     9901 agagaaaatg atgccgacag atgaagttga aacgacaggt gctccagaac agccacaaga
     9961 tgagctgttt gaagaagcaa aagcacttgt cgttgaaatg caaacggcaa gtatttccct
    10021 tctccaaaga agatttcgga tcggttacaa ccgagcagca cgtttagtag atgaactgga
    10081 agctcatgga gttgttggcc cgtcagaagg aagcaagcct cggaaagtat tcattgagca
    10141 ggaagaaaca tcagaaacgt tgccggacga taatgacatg taaaacttgt gtgatgaagg
    10201 gttgaaaaac gaataaccaa taaaaagaca aggcatgatg ataccaaaag aaaaaatggt
    10261 ttttttccat cttttctaat acggtatgat gtcttgtctt tttagttttt gtgagattaa
    10321 agttctattc gatttatttg tgaagtattg cattaaggaa atgaatcatg tatagtatgt
    10381 agcgaaactt tgagaaaaag agggaaaata ctttggaaaa acagtccatt tatggcttga
    10441 cgaatgaaga attaatcaat tggtttatag aaaacggcga gaaaaaattc cgagctgccc
    10501 aagtttggga atggttgtat caaaaacgtg taagtaattt cacagaaatg acgaatctat
    10561 cgaaacaatt gatcgaaaaa ttatcggagc attttatcat caatcctcta aaacagatgg
    10621 tcgtccaaga agcttcggat ggtacagtaa aatatttgtt tgaactccct gataaaaaca
    10681 tgattgagac tgtactgatg agacaagaat atggcttgtc agtttgtgtg acgacacaag
    10741 taggatgtaa tatcggctgt acgttttgtg caagcgggct gctcaaaaag aaccgtgatc
    10801 tgactgctgg agaaattgtt gcacagatca tgatggtcca acattatttt gatgagcgca
    10861 atcttggcga acgcgtttca catgtcgttg taatggggat cggtgagcca tttgataatt
    10921 acgacaatgt aatggacttt ttgcatatca tcaatgatgc caaaggattg gcaattggtg
    10981 ctcggcacat tacagtttcc actagtggat tagcacacaa aatcaaggaa ttcgctaata
    11041 acggcttgca agtcaatctg gctatttcct tgcacgcgcc gaataatgag gtgcgaacat
    11101 cgatcatgcg gatcaaccga agtttcccaa ttgagaaatt gatggaagct gtggatgagt
    11161 atttagagaa aacaaaccgt cgaatcacgt ttgaatatat catgttgaat caagtaaatg
    11221 atagaccaga gcatgcgcaa caattagcag atctattaaa agataagaaa aaattagcat
    11281 atgtcaactt gattccatat aatccagtca gtgaacatga tcaatattct cgcagtccaa
    11341 aagctgatgt attgagattt tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac
    11401 gtaaagaaca tggtacagat attgatgcag cttgcggaca attgcgaagc aagcaaatga
    11461 aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt caattaccat aaaaggagca
    11521 gttttcttaa ttaaggaact gttcaactta aataagagaa aagattctat agcgaaagct
    11581 ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg
    11641 acagcctgct tgatgggggt tttagcaatt atttaactga ttttcttata tccttgctgg
    11701 ctttcttgtg tagcatgata agtttctacg tcaagaatgg cctgtttaaa tttttctcgg
    11761 gtgatttcat aaggcacatg tttaatatca tttgttgcca tggctttatc taagattgct
    11821 tctaaatcgg tcgtgacatt gagattcatg tcggttaacg atactggaag ttggttcttt
    11881 ttcataaaca catagatttt atcaaacaaa tcgaactgct gatccatcat caataaggtc
    11941 agtacaccat aactgacaga tgctccgtga aggtatccct ccattttttc tagaacagtc
    12001 gtaccgtaat agaatgagtg tgccatagca gaattatagt tattgttaac taaaacagaa
    12061 accaatccgg tagtaacaat gatatccgaa acgacttgtt caattgcata tgagggacga
    12121 tcgtcggcac tatctttcaa tgcttgcgta ccataagtca agagacgatc aatgcacatt
    12181 tttccgattt ggattcctaa tctatccgtg tagcctagat catcgccacg agcagaaaaa
    12241 gtagattcga cttctttgga taaagcatcg ccaatacctg cccataagta tttatcaggt
    12301 gcttcggcaa taattgctgt atcaatgaag gtgtgtacag gtggttttga aggaaagaag
    12361 agtccgtcca ttacgccttc ttcgttatag atgacacata ccgcagtgac gggcgaacag
    12421 ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta gtttttcacc aatgactttt
    12481 acagtatcgc aagcacggcc accaccgact gcaaacagga cgtctgcttt ttggacactt
    12541 tcatgctgga tcaactgttc aatatttcca taagttgaat tacccccgta ccagagactg
    12601 gcgacaacct ctatttcctg tgtcaaagct acttttattt tgttttctgc ctttgaaagt
    12661 gcagtcttcc ctccaatcag cactactttt gtcccatatt ctttgacgat tccatttgct
    12721 ttttcatacg catctgttcc aatggtataa tttggtaaga aaacactttg actcatattc
    12781 atttccttct ttcttcgttg aaacgggtta aaattttaaa gagtgatcga tctgactgcg
    12841 gctgtagatg gagtagaggt gaacctaaga caggataagt gatgaataag gtggcatgaa
    12901 caccttgaaa tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga
    12961 caataatgaa tttttgttcc tcttagtatt tctaccgatt ccctttgctt ttttgtaatt
    13021 atctaaaaat tctaaaaatt aagattatta tatgaaaaca ggttttattt gtcaattcat
    13081 aattggtttt ttttaaagca aaagataatc tatatcatga tgaacaaaca gagttttttc
    13141 ctccaattga tggctttcct gatctttcct attttgaata aagtgtttct gtataatttt
    13201 tttattttat aagtaagata aataatgaaa gaatagagat actgattggt agctaaagat
    13261 ttttgagcga gaacatttcg ttttttcaaa aggactgtta gaataagcaa gaacaagggg
    13321 gaagacgtat gataatcagt gaatttgacc gtaataatcc agtattgaaa gatcagcttt
    13381 ctgatttact gagactgact tggccggaag aatatggaga cagctcggca gaagaagtag
    13441 aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt agaccaagat gagttagtag
    13501 gatttattgg tgcaatccct caatacggta tcacaggttg ggaattgcat ccattagttg
    13561 tagaaagctc ccgacgaaag aaccaaatag gtactcgatt agtcaattac ttagaaaaag
    13621 aagtagcttc cagaggagga atcacgattt atttaggtac ggatgattta gaccatggaa
    13681 caacgttaag tcaaacggac ctgtatgagc atacatttga taaagtggct tccatccaga
    13741 accttcgtga acatccgtat gaattctatg aaaaattagg ttataaaatc gtaggtgtct
    13801 taccaaatgc aaatggctgg gacaaaccgg atatttggat ggcaaaaacg attattcctc
    13861 gaccaaattc tcaataataa aaagaggttg tgacagaagt aatcagcttc aagaaataag
    13921 aaggaattta cgaaaattac ttcttgaatt tttgtgaaat tccaacttat ttccgaagaa
    13981 gctacttttg gaacaccgtg taccagaggt tgtgaaaaag tcgcttagtt tcgagtagta
    14041 agaaaaattt tgtgaaaatg gctttctaca tttttgcata aatttggctt aataccgaga
    14101 aactggc
//
LOCUS       DAFCNS010000068        14096 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFCNS010000068 DAFCNS010000000
VERSION     DAFCNS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.070"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(142..1086)
                     /locus_tag="LNE23_002134"
     CDS             complement(142..1086)
                     /locus_tag="LNE23_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBK6931968.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1220..2611)
                     /locus_tag="LNE23_002135"
     CDS             complement(1220..2611)
                     /locus_tag="LNE23_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBK6931969.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            2737..3147
                     /gene="tagD"
                     /locus_tag="LNE23_002136"
     CDS             2737..3147
                     /gene="tagD"
                     /locus_tag="LNE23_002136"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HBK6931970.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            3323..3736
                     /locus_tag="LNE23_002137"
     CDS             3323..3736
                     /locus_tag="LNE23_002137"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6931971.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            3893..4150
                     /locus_tag="LNE23_002138"
     CDS             3893..4150
                     /locus_tag="LNE23_002138"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6931972.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            4237..5475
                     /gene="istA"
                     /locus_tag="LNE23_002139"
     CDS             4237..5475
                     /gene="istA"
                     /locus_tag="LNE23_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBK6931973.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            5465..6205
                     /gene="istB"
                     /locus_tag="LNE23_002140"
     CDS             5465..6205
                     /gene="istB"
                     /locus_tag="LNE23_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBK6931974.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            6615..6779
                     /locus_tag="LNE23_002141"
                     /pseudo
     CDS             6615..6779
                     /locus_tag="LNE23_002141"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6846..8273)
                     /locus_tag="LNE23_002142"
     CDS             complement(6846..8273)
                     /locus_tag="LNE23_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBK6931975.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEVRKIIRK"
     gene            complement(8270..9412)
                     /locus_tag="LNE23_002143"
     CDS             complement(8270..9412)
                     /locus_tag="LNE23_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBK6931976.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            complement(9416..10411)
                     /locus_tag="LNE23_002144"
     CDS             complement(9416..10411)
                     /locus_tag="LNE23_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6931977.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            complement(10525..11514)
                     /locus_tag="LNE23_002145"
     CDS             complement(10525..11514)
                     /locus_tag="LNE23_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6931978.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            complement(11486..12316)
                     /locus_tag="LNE23_002146"
     CDS             complement(11486..12316)
                     /locus_tag="LNE23_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6931979.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            complement(12354..13745)
                     /locus_tag="LNE23_002147"
     CDS             complement(12354..13745)
                     /locus_tag="LNE23_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK6931980.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
BASE COUNT         4785 a         2554 c         2091 g         4666 t
ORIGIN      
        1 aagttctctt tttcatgtaa aatatttaat tatatatttc ctaaataaaa agtaaaacaa
       61 gaattaaata gttggttata caactttaat tcttgtttta ctttttagaa ttatttatcc
      121 aaagatgcaa tcattttttc atcaaatttc tgtctttaaa taccgttcca ttccgctcaa
      181 tatatcaaat ttaggctgat agcctaaaga acgaattttt gaaatatccg ctaaggaatc
      241 acgaatatcc cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg
      301 atcaattgaa tggatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg
      361 ctgtcctaaa gctttttctt ccttggcaac aagtaaaagt gcttgtataa catcttctat
      421 ataaacaaag tctctagact gactcccatc gccaaataaa gtaaattccg ctttttctcc
      481 tgccaattgt tttttgtaac gatccactag aatcgaaatg actccgctat atggagagtt
      541 tggattttga tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata
      601 taaatgacag taatccaaaa catattgttc tgctgcaaat ttatcgatag catatggtgt
      661 taatggacgg atgactgatt cttctttttt tggcagtgtt ggttcatcac cataaactgc
      721 agctgatgaa ctaaatacta atcgttttaa attagattga tatttccgtg ctaactcaag
      781 caataaaagc gcactgtcaa aatttacacg atgtgtttct acaggccgtt ctacagaatc
      841 tgccacacta gctacagcag ctagatgaaa aatataggca aactgatatt ctttcatgat
      901 tctttccatc aattgagaat ctgatacatc cccctcaatg aaagtcactt gtttatccat
      961 atccaagttt tctttttttc ccattgataa atcgtcaact actacgattt tgttttcttt
     1021 tcccaaataa ttcgctagcg ttgaaccgat aaaaccagct ccgccagtaa tcaatatagt
     1081 gtccatcaaa ttcactccaa ttgcacattt tcgtatcaaa taatattagt ttaccatttt
     1141 tatattgtaa aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt
     1201 catataccta aaaatagttt caattagtta ctccttcttc tttagatata gttttttgga
     1261 tagaacccag atatccccaa aaaataaatg aacctatcgt actaaccaaa atgagttctg
     1321 tgataaataa tgccgaaact gctattgtaa gaatcattaa aatagcaaaa aagtgcttat
     1381 cttttactag atttggttgt gtgaaacaat aacgcaaggc tttaaagcat tggataaata
     1441 agaaggttaa caatgtcaat agacctaatc ctcctgtagc agccatcact aagaaaataa
     1501 aattatgagc agttaaagta tcttttccca ttaatgtata tggtagttta tcatgtgcat
     1561 attcaatata gtagcctgga gaacctccgg atatcggctt actcttgaat atttcaattg
     1621 cacttttcca aagttcaaac cttaaattag aaacatcccc gctatcagtg tcagggcgtt
     1681 ttaaatcaac tttattgctg ctagttgttc ctgatgaatc agatgtatct ttttttactt
     1741 ctgtaatatg cggaacatgt gcccatgcat gagcagttat atctgttaca taatacatcg
     1801 caattatact tagcaatcct actactaatg cagttagcca tctttttaaa gtattagttt
     1861 taagaaaacg actactataa cttgtaaaga aactaaaaac aaaagttgtt attaataaac
     1921 aaactaatgt ggtgcgtgaa cccgataaag caataaacga gaattgaata aaaatattaa
     1981 ttacaaaaaa tattttaaat agaagattta aatgcttgta agtgaccaaa taatatatag
     2041 caataatgat gacgattaat gatataaccg caccaaaatt tggatctgta tatacaccaa
     2101 ataaacggtt gcctaaccac cctaaacgaa ctgggtaata cttatatttt attggaattt
     2161 ttatgccata ttgaatgaca aaccagtata atgatactaa attagctaaa aaccatacca
     2221 atgaaagtat ccaggtaaat actttgaaaa aaaagttact actatagtct ttgccaaact
     2281 gataaatcat aaagaaaaag attaattgcc acatgattaa cttaatattt tcgctatatc
     2341 catattttaa attcattaaa ctgcttacta gcatcgcgcc aataaaagca attaatagcc
     2401 aatcatattt ttttagattt ttttctctaa atgcaataat tgcatctata atgatcaaga
     2461 caactcccgc tattccagca ggccaaaaaa gtaaagtatc atatctagta ggtaatagta
     2521 aatacgggcc tattgctcgt aacattaaaa taaaaccaaa ataaataaaa taccaacctt
     2581 tcacatattt taaaaatctt tcattcaaca tttttcctcc atccacctat ttctcataaa
     2641 tatttcttat cctttttagg ataaactggt cctttcaatt ctaacaaaaa aatgctaaac
     2701 taacaatgta tattaatgaa tttcgagagg tgaaagatga aaagagtaat tacttacggt
     2761 acctttgatt tgcttcatta cgggcatatc aatttactga aacgagcaaa acaatacgga
     2821 gactatctta ttgttgccct ttctacagat gaattcaatt ggaatgcgaa acaaaaaaaa
     2881 tgttatttta gttatgaaaa aagaaaacag ttacttgaag ctattcgcta cgttgattta
     2941 gttattcctg aagaaaattg ggaacaaaaa gtaaaagata taaaagaata ccatgcagat
     3001 tatttcgtga tgggcgatga ttgggctgga cattttgatg aacttgaaca actaacagat
     3061 gcacaagtga tttatttacc aagaacacct gagatctcta caactcaaat aaagaacgaa
     3121 cttaattctt taaatagtga taaataatat tttttaaaca ataaaagaac aatatatctg
     3181 gataagacgt aaaaaaatgg ctctataatt tctaggactg atagaatttt ctgtcggtcc
     3241 tagatttttt gtaaaaaaaa acagacataa aaaaattccg atagaataga gtcgtctaaa
     3301 acatcaatct gaaaggaagt ttttgatatc catttctcat tctatcaaag agctcttcac
     3361 tattaaagat aaaaatattg aaattaattc ttttcaagaa actttttata aggacaagaa
     3421 agccatcgta atcgaaggtc cattagttcg cacttttaga cattgtctat tttgtaaaga
     3481 atctgcaaag cagatcgaga aaaacggcaa gaagatttca atgattatct taaatcgcag
     3541 tgggaataaa caaacctatt tgcgtcttag aaaacaacat taccactgcc aatcatgcgg
     3601 gaaatatttt actgctcata cgtatcttgt cgctccttca tgttttattt cgaaacaagt
     3661 tcattacacg atattagaag agttgaccga gcgtcaagca ttaaagacaa ttgcgaaacg
     3721 ttgtgatttt cagtaacgac agttcaacgg acattatctg ctctatcggc aacgctgcga
     3781 cagaagctag actatttacc tcattgttta ctattcgacg agtttcgctc tctttcaatt
     3841 ttccatggga agttctcctt ttcttgtatg aattgagaga cagggaagct ctttgacatt
     3901 ttgtccagcc ggagaaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc
     3961 tcatatgggg gttcgataca ttgtcacaga tatgaatgcc ccttattttt cattagtcaa
     4021 agaacccttc ccaaatgcac aaatcatcat tgattgcttt cacattgggc aacacttgaa
     4081 caagtttttc gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aatgtcaacg
     4141 gcacctttaa aatgtaggaa aacggcaatt taaaattgtt ggtttaccta catttttgta
     4201 tgctcttctt atcccaaatg aaatggaggc tcagggatga gaaaagacat acgcgaagga
     4261 gtgatgatct acgtgatcaa tgaaatcaaa cccaattatg ctgcactagc aaaacaatac
     4321 gactgcgact accgaacagt taagcatgct tacgaagaag cccaagttaa agaatcaaaa
     4381 cctccagaac gtaagaagcg tccaagtaag ttagatccct acagagaaat catccaagac
     4441 aaaatcaaag accaatgcag tgcttacagc attttcaagt ttattgagca caaaggattt
     4501 ggaggaagct acagtttagt aaagacctat tgtcgccaga ttaagcaggc gaaagtacac
     4561 aaggcaaccg tccgagtcga acatagccct gggctttctg ctcaggttga ctggaaggaa
     4621 gatatgcgac taatcagtaa tcagggcgaa atttttgagt tcaatatctt tttgtacgtc
     4681 ttgccatatt ccaagaaaaa atatgtcaca ctaactttcg accgaaaaca agacacactc
     4741 tttgagtgct tagatgatgc gttttatcaa accggtggcg ttcccaaaga aatctggttc
     4801 gataatatga agacagtcgt tgaccattct cgtagtcagt tttccaaacc gcatttcaac
     4861 aaacgttttt atgctttcag taaggatgcc ggcttcacac caattgcctg tcggccattt
     4921 cgtccgcaaa caaagggctg tgtcgaagct ttagccagaa ctgttgaccg cctacgggtc
     4981 tataatcatg aattttatga tgccgttgag ctgtgtcatt tagtgaataa cctttgtgtt
     5041 gagttaaata acgaggtttc tcaatcaact gatcaaacac cagatgaacg ttggcagacg
     5101 aacgaaaaag agcacctgca tccataccaa aaaaaccttt tgaatcccta ttttgaagac
     5161 tttattcgcc gtaaagtcac caaggaagaa atggtcattt tccgtaaatg caggtactct
     5221 gtggcgcctc gctatatcgg taaagaagtc gaaatcgaac tctccgataa agaagaccaa
     5281 gtacaaatct attataacgg agaactgatt cggtcgcatc cattaacgac aaaacagctg
     5341 aactaccacg aggaagacta tttccaaatc ttgaaatccg atgtcatgag tcacaaagaa
     5401 gacgatggaa tcagagcgta tactcgtgaa agtctcaagc aatatgatct ggtggaggtt
     5461 gccgatgaag aatagccatt accaagaact gatgaccaac ttagagactc ttggtttgaa
     5521 tcatatgcgg gaatatgttc ctaactacat cgaagtggcc aatcgagagg agctttcttt
     5581 aacggaagct cttctcgagc tgacacgcag agaagttgat catcaaaata agcaaaaaat
     5641 gaatcgtgtg gtggcacgcg cccgcttttc caagagaacc agcctggaag agtttgattt
     5701 tgccttccag ccaagtatca ataagcgcga agtgctagac ttaaggcaca tgggctttgt
     5761 ggaaaaacat gagaacttgg tttttatcgg cagtcctggt gtaggcaaga cacatctgac
     5821 gattgcattg ggaatcgaag cctgtcgtca aggctatcgg acactattca tcacctgtca
     5881 tgagctgctt ttgcgtctgc gaacagccta tgaaaaaggt accctagagc gtgttctcaa
     5941 acgatacgct cgctatgacg tcttgattat tgatgagatt ggctatcttc cgattcaaaa
     6001 acaggaagct gatttattct ttcagcttct tgccatgcgc tatgaacacc gctctacgat
     6061 cattacgacg aacattgtct tatctcgttg gggggagttg ttccaaagtt ctgagatcgc
     6121 agctgccatc cttgaccgtt tagtacatca cgtcaaggtg ttcaagatca caggcaagtc
     6181 ttatcgtatg agagacaaga tctaaagtgt cgattgtcga caattactat tgccgaaaac
     6241 ctacattttt atcctgccca aaacctacaa ttttaagctg cccttgacat caaaaagaca
     6301 cttcacaagc taagtgctat cgacaattga aacctttgta taaattactt ctataatcag
     6361 atgacgagtt ggattataca acattcaaga aatggcagaa ttttcaatgg cgctatttaa
     6421 cagaaagtga agtagttgat tgtctaccct caatctctta tgaacttaaa atggcttagc
     6481 agtattatca aaagctgctt aacaattatc acaagaaaca gctagctact tcgagaaatg
     6541 accaaagaag ttccctcgaa acttcactat cttaaaaagc ctttttcata tctgaaaaag
     6601 gcttttaact agcattttcg aaaaagtatt ttaatacaaa attagagaat cttcgcacac
     6661 atatcaaaac attgaaacga tttgcttatg gattttgcag ttttaccaat atgcgcttgt
     6721 ggatttttct aatcaacggg ttaataagct atgcattaaa agctagtaaa tcttactaac
     6781 tttgttgatc ataaatgacc ttatcagccc gatttgacaa agaactactt ttttacaaca
     6841 ttattttatt ttcgaataat ctttcttact tcatttcgta tatctggaaa aagaaaataa
     6901 acaaacggaa gataaataat gaaaatcatt attgatttaa ttgccatatc taaaaatgaa
     6961 gaagagaata attgtggaag aaagtaatag gccgaagtag tcaaagtaag attgactata
     7021 aaaaataaga caacgttctt cccaaagaaa taggatattt ttatttcttt tcgtacaaaa
     7081 aatactctaa agaaaaagac taataattct actaccacga gtacgattgt agccgctaca
     7141 gcgccataag taggcacaaa agctacattt ccaataatac ttacaatcgc accaatgatc
     7201 attggataag catactcctt atctttttct atagcaattg caaattggtt agcaaaaact
     7261 ccaccaattg gactaacaac tataattaga caagaaataa gcatgtttat tgtcatcggt
     7321 ttgaaattct ccccaaaaaa ccatggaaca aattcttttg tattgataaa caccacacta
     7381 aaaagaatta atgcaataat aatcgtgtaa tcaaaagatt tttttgtgta aactaaaata
     7441 ttttctgatt ttccctctgc ctgtgcttta gatattttag gcaacataac aatagtaatt
     7501 gatgttaaga ctgccaatat aatacgagca attttttgag actgatcata ataagataat
     7561 gctgttggat ccacaaaata cccaactatc gttttgtcta aagttgtgta cacttgcata
     7621 aacaactgag gagtcaaaat aataaaagct ggtttccaca actgtttaaa aacattagag
     7681 ttaaagagtg gtttacctac ttttccgtgg atactcaacc agaaaactaa acttcctaaa
     7741 aagttagtaa ttgaagtaat gaacacataa atccacaaat catctttagt tgaaacaaaa
     7801 aggattatta acaataccga tgataatttg ataactgtat ttctaataac cactttcgaa
     7861 aattcttcaa ttccttgaaa aaaccaagaa atatctaatg ttagccctgc aataaatatc
     7921 aattgaatta agtataaatt tttatacttt acatcaaaaa aataaatata ggctatatag
     7981 agaaaggaaa cactcaagcc tattaaaaat tgaagaaagt aaatattcca gaattgtaaa
     8041 gatagtgctt ttttatcttt ccttgattta gcgatatttc gaatacgaaa aggattcatt
     8101 cccacagcaa tgacagtcat taaaaattgt gatattgaga aaacataccc ataaatccct
     8161 aaaccagttg aacctattct acgactaagt ataggtatag taagtaaggg caaaacaatc
     8221 aaaaagactt gatatatcgc attataaaat atatctttta ctgttttttt cactttatat
     8281 ccctctctaa caactcaaga accttttctg tacttgcccc tgtttcataa tttccaatat
     8341 aatcaagtaa ctcttgagaa cttttcaaaa gcttttcttg ttggttttta aaaacatcaa
     8401 caaggccata actatcttgt acgcttataa ttggtaactc acttaactgt atataaaaac
     8461 ctctttctaa gctatattct ttttcatcat agttaaaaag aataattggt ttttttgtta
     8521 gcgcaaaatc gaaaaaaata gatgaataat ctgtgattaa aacatctatc tcttcaaaaa
     8581 gcatttcaag aggaatccca tcattaatta aagtaatttg atgttttttc atatattcta
     8641 tacttttaga atcagttgct aatttactat gaacattagg atgaaattta gtcattaaat
     8701 ttaattcttg ttgttgcttt ttaaaaaatt cacgtaagtt atccacacct aaccattcta
     8761 gttgcgtaat tccgttgtcc tcaatattat ttcgaaaagt tggagcaaat aagacattga
     8821 tttgtttctt tttcaagtca aacacttgct ctttactaga ggctaattca tcgtttctag
     8881 gagttcctac ttctaatatt ttagaaggaa cgatattgaa tgctgaaaca tacttctcag
     8941 aactatagcg attaccagat aataaatatg taattgactc actatctttt ttcgcatagc
     9001 ttaggtaacg tttaggtaac ttatcttttg catcaaattc aatttttttt aaaggcgtac
     9061 cgtgccatgt ttgaaaataa aattgttttt tcctttttgc atacttaatc gttttccgac
     9121 tatcgtctat ccaatactta gctgtataca aataaaaggc agctaaaaaa gaatcggaat
     9181 caattctttt aatataatct ggtaaatcag ttataatcgc ttcttttttg attaaccaaa
     9241 taattctgta attcaatttt ttttcatgaa ttttctctga aataactctt ggatttccag
     9301 aataattacg cccgccaaaa cttgaaaata caattaaatt tttttgaata ggtaatattt
     9361 taataaaaac cgggaaaata ttccttgaaa aaaatttttt tatattcttc atctcttacc
     9421 tcacagattc tatcttaatc aaaaatttac ttgctatctt actacttgac atatagtaag
     9481 gcaagttttt atttattaat aaaaacttta ctaatttttt taattttggt gaaaagtcgg
     9541 gatcaatttt cacttctttt tcaaagcgtt gtaaaatatt tatagaacgt aaataatctt
     9601 ctttattggt atagttattt ttaatcattg tcatcatcat ccttattgct tcatagacat
     9661 agcgagttct aacattaatt gataattccg gataaaactt atctatgaat actagtatat
     9721 cttcatggac ttcaaaagaa tcaaaaattc taggattgaa gcttcctctc agtatacttt
     9781 tagggctttg gtaatagtga tatttagcag atgaattgac agcaactttt tcggaatgat
     9841 cgatcaacaa ataagtagta gccttatctt ccattaattt acccagtggg aatcttatat
     9901 tatcaaataa atttctattg aataatttat tatttgcaga atagccaatt ttattattta
     9961 taaataagtt tttcattgct tgttctctat caaatatttc ccattttctc tcagcaattg
    10021 tatgttcttt tagttgttct aaacttgaaa tcttttttat ttttagcacc gagatatctg
    10081 ctccataagt ttgcgtacca aaatatagta actcaagata gtctggttct atccaatcat
    10141 cgctgtcaat aaagcacaca aaatctcctg ttgcagaatc aagaccagaa ttcctagccg
    10201 cacttaaacc accattcact tgatgtatca cttttaatcg catatctttc tttttatatt
    10261 cttctgcaat ttccccactt cgatccgggg aaccatcatc gactactatt atttcaatat
    10321 ttgaatgtgt ctgattggaa atagattcta agcagttttc taaatagttt tctacgttat
    10381 atgcgggtac tataacacta attttatgca ctctcttcac ttcctaaaaa tcatcttttt
    10441 tatttttaca aatgtatcat taccagtaac aattttagtc ttccgaagtt aataaacata
    10501 aaaaataaca tatcgaaaaa aactttattt ttttagaact tttttatata aaataaaata
    10561 agttttagga ttaattaagt ataaaaataa agctaatttt ttagcaattg atatctcatt
    10621 gttacttctt atttttgaat atatactttt tatttctctt ttagactgat tataatattt
    10681 ttgtttttct ttctgatttt gatttttgcc agtaatttta aaccctgttg aaagatatcc
    10741 gtctactaac cgagcattgg ccaaattaat aagtcgagga taatctttct ttacttcatc
    10801 aactaatatt tttctctgca aaatattatc atactgtttt gaattaaaag actgataaac
    10861 gatgctatcg ttattttgat aataaaaaat acctgaaaat ggcaaaatat caattttact
    10921 agccgcccta aaagcttcat acataggaac catatcctca tataatttat tttctggaaa
    10981 aactatatga ttgaataatt cccttttaaa aaatttattc catacgtaaa agtccactgc
    11041 cttacttcta aacatttgct caattgcttc ttcacttgaa caaccaatct tcttttcggg
    11101 aacaaataaa tttgcatttt ctatattttt tcctttactt aatccaaaag caatgatatc
    11161 ggggtgattc ctatggtagt ctttcaaaac attattaaaa caatcaggta aaaaataatc
    11221 gtcactatct aaaaatccta ccaaatttcc agttgcttct tttatcccat agttcctagc
    11281 aactgatgga ccagcgtttt ccaagtgata acatgctact ttactctcta gattagcaat
    11341 ttcgtcacca atgtcactac taccatctgt agatccatca tcaattaaca agatttcaat
    11401 attgggataa ccttgcgtta agacgctgtc tacagctctt cttaaagtat tctttgcatt
    11461 gtaaaaaggt atgattatag aaagtttagt ttgtttttct aagttttgat tcatatactt
    11521 ttcccctaat tttattcggt aataaactaa ttacaagatg tccgcaaata actatcagaa
    11581 aatcttttag tgatcctaga ccatctttcc atacgcgata tcggccttta attcctggta
    11641 aaagatattt acttcctcca cgtctagcat acatgtcagc acctgctcta gcatgtacta
    11701 gaatctcttg gatattatat cctttatatc ccgccttcaa tagtcttacc cacaaataat
    11761 aatcttcaaa tccaattaat gggcgatagt ttcccacttc taaaatagca tttttatcaa
    11821 acatgactgt catatgatta aaagggtttc ttcttttcga aaacacacgg atttcatcat
    11881 tatactcagg tacaatcctt cttccgataa cattgctaag tgtgccagta aattcttcaa
    11941 tattactccc aacaatcgct atttctggat attcttggaa gatctctaat tgtttttcca
    12001 atctgtgtgg ttccatgatg tcatctgtat ccattctggc cactaagcga tttttagtat
    12061 gttctaatcc agttgctaaa gcaatcccca atccttgatt tttttctaat tttacaactg
    12121 taaacaagtg aggaactttt tgctggaaat aagtgacaac tgcctgaagc ttctcattta
    12181 tagggccatc ataaaccaat acgatttctg ccggcatcac cgtttgctca aagatactag
    12241 ctaatgcttc ctttaaatat tccggctttt ctttatcata caaagaaagc agtatactta
    12301 tatttttatt ttccaatgtt ctctcctact tctttgctca aaatttttcc aatctaacga
    12361 tacgtttttt cttttggaaa tgcactttct gctaatgaac tgatattttc atcttcatct
    12421 agtccttgtg aggacatctt atcaaataag attttgattg tttgaagtaa aatcttcaaa
    12481 tctaatagta aggaatactt tttgatatac aacaaatcaa aacgtagttt actgttataa
    12541 tctgatacat attttccgta tacttgtgca tatccggtaa ttcccgcacg tacattgtgt
    12601 cttaagtaat aataaggatt ttcttcattg aactgatcaa cgaaaaatgg tctttctggc
    12661 cttggaccga caatcgacat atcgcctttc aatacattaa agatttgagg taattcgtca
    12721 aaacgtacag cacggataaa tttacctact ggtgtcacac gtgcatcgtt acttttagcc
    12781 aagactggtc ctgattttgc ttcagctgtc gcactcatgg aacggaattt atagattgaa
    12841 aattcctttt gatttttcgt aatacgtact tgtttataga tgattggtcc aggtgaagtt
    12901 gtttttacca aaatcgctgt gattagcata aaaggtgagg cgaggatcag taaaataagc
    12961 gaaacgatga tatcaaagag acgcttgaat agttcgtatt ctggttggat tctgaaccca
    13021 gaagcttcaa tgatgctttc atcttcaaaa ttcataatat tcggatttat cattgtcaga
    13081 ttctcaaaag tcgtatttaa aaataatttc ttctcttttt tagtcactaa ttgataaatg
    13141 ttgatttttt catcttctgg gattttagaa gccaaataga cgatatccat atcatcgatc
    13201 aatttttttg tattttcgta ataattatcg attgccactg cttcaacctt atgtttattg
    13261 tttttcttag attgaaaatt ttggatagct ggtgcgacat ctttctccaa acccacgata
    13321 aggactttct tatcacgact gactcttaag tacagtttgt aaacgactat ccggtaaaca
    13381 ctaagtaata tcgtacctag gataaacgag atcaaaatga cgccacgcgg aaatgcaaac
    13441 catcggccag caaaagtaac taccatgatc cccaatgcta ttagtacttg tccaataaca
    13501 gtaatgaaga caatatcact gatcattcgg ttatagaata cataagctcc taacaaaata
    13561 ttcaacgcaa taaagatgat ggaaataaaa attgctgaat gctgaaaggt ttctaagttt
    13621 ctcactggta cttctccctg gaatttcagc aaaaaactaa gatagatcga tacatttaat
    13681 acaagtacat ccataagaat aatgaatatt ctgcgtgccg cattccactc cccattttta
    13741 ttcatatata cactcctaac cataaaataa tgcctctatc atcttaccat agcaaagttt
    13801 aaaaacctag tgaataacca aattgtaaag gaaattaaaa gaaacgggga aaagcgttta
    13861 ttgtctgttt atatgtgtat ttctgcataa cagaaagttg tctcagaagt cccatcactt
    13921 cctttatgct gaactagtat tctcctgcta attccttatt cacaagttac aatattcata
    13981 taacgcgaat tgtaaaatta agttagaaaa ataaaaaggc atttatggta cactcaaatt
    14041 gtatttccga cgaaagaaaa caaaggagtg taaccataaa tgacctacaa acatct
//
LOCUS       DAFCNS010000069        14020 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFCNS010000069 DAFCNS010000000
VERSION     DAFCNS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.071"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            282..2021
                     /gene="yfcC"
                     /locus_tag="LNE23_002148"
     CDS             282..2021
                     /gene="yfcC"
                     /locus_tag="LNE23_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBK6931981.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2178..3362
                     /gene="iadA"
                     /locus_tag="LNE23_002149"
     CDS             2178..3362
                     /gene="iadA"
                     /locus_tag="LNE23_002149"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBK6931982.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3473..3676
                     /locus_tag="LNE23_002150"
     CDS             3473..3676
                     /locus_tag="LNE23_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK6931983.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3777..4439)
                     /locus_tag="LNE23_002151"
     CDS             complement(3777..4439)
                     /locus_tag="LNE23_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6931984.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4452..5954)
                     /locus_tag="LNE23_002152"
     CDS             complement(4452..5954)
                     /locus_tag="LNE23_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6931985.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6502..8181
                     /locus_tag="LNE23_002153"
     CDS             6502..8181
                     /locus_tag="LNE23_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK6931986.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8308..8787
                     /locus_tag="LNE23_002154"
     CDS             8308..8787
                     /locus_tag="LNE23_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBK6931987.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9006..9686
                     /locus_tag="LNE23_002155"
     CDS             9006..9686
                     /locus_tag="LNE23_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK6931988.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9694..11025
                     /locus_tag="LNE23_002156"
     CDS             9694..11025
                     /locus_tag="LNE23_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6931989.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11040..12002
                     /locus_tag="LNE23_002157"
     CDS             11040..12002
                     /locus_tag="LNE23_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBK6931990.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12113..12451
                     /locus_tag="LNE23_002158"
     CDS             12113..12451
                     /locus_tag="LNE23_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBK6931991.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12530..13237
                     /locus_tag="LNE23_002159"
     CDS             12530..13237
                     /locus_tag="LNE23_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBK6931992.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13340..>14020)
                     /locus_tag="LNE23_002160"
     CDS             complement(13340..>14020)
                     /locus_tag="LNE23_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HBK6931993.1"
                     /translation="FFYCFFPNQISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQF
                     PLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREK
                     IDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLF
                     RKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKG
                     YENKTKRM"
BASE COUNT         4498 a         2440 c         3030 g         4052 t
ORIGIN      
        1 gaaattcaat ttttcaatga ttgtaaaatg aaaaaaacag gacgagattt tctctaatct
       61 aaaaataaaa aaagaatttt aaggaaaaac agagaaataa aatacttaat ataaatggaa
      121 agagaaaaaa ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag tgattttcta
      181 ataaattatt catgtggaaa tgtcattttt actgtataat tcatttgatt atccattttt
      241 tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa acaaattctt
      301 caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat gtcattattt
      361 ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga aagttcagca
      421 cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact gttttacgtc
      481 aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat caattagaag
      541 aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa ggattggaaa
      601 aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa atctccttga
      661 cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct aaaaaattgc
      721 ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc ttaaattttg
      781 tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc gctgcattga
      841 tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc gacgcaggta
      901 tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg ctattattct
      961 ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt ccattctcaa
     1021 tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca gtaaccgtga
     1081 cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt agcgtagcgg
     1141 tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga ttgattatgt
     1201 gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa aaaatccgta
     1261 aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct catatcaaaa
     1321 agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta ttagagatgt
     1381 tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac attccagaaa
     1441 tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt cttttcaaat
     1501 tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca gctgatttag
     1561 ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg ggaggatctg
     1621 atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg gcgttgaccg
     1681 gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg ttcaacttgg
     1741 ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct ccactggcag
     1801 atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt tcaggtttta
     1861 tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt gcacggatcg
     1921 aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt gttttaggaa
     1981 ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga cctgtaaata
     2041 aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg gtaaaatcaa
     2101 aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag ttatacttaa
     2161 agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca ccagattact
     2221 taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa gaacagctag
     2281 aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa gtactaaccc
     2341 cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc ggttatcaaa
     2401 atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg acagttgtcg
     2461 gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc aaagcgaaag
     2521 gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga ttgccggtaa
     2581 aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt atcggaatcg
     2641 gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag ttcgctcgtg
     2701 cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa agctgggatc atcaatgtcc
     2761 acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa gaaacagaaa
     2821 ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta tttgaagctt
     2881 gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa gatcctgatt
     2941 tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga ttgatcgagg
     3001 aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc ttgccttatt
     3061 ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca ttgctagtag
     3121 ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta cgagctataa
     3181 ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa attggtgctg
     3241 atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt atcgctaaag
     3301 gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa aaaagttttt
     3361 aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct gtatctgttg
     3421 ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca caatggcaga
     3481 tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa aagaagcata
     3541 cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat cactaggtgc
     3601 tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt tttctcgaaa
     3661 agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa aaaatccgaa
     3721 ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc tgttttttaa
     3781 acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca gctttttgct
     3841 acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc gatttcttgg
     3901 aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg ctcatccgct
     3961 acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg ctgctgacct
     4021 cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac acgagccagt
     4081 cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc cattgctgct
     4141 atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa aataatcgag
     4201 acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc ttgatagtat
     4261 acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt cgtttttcca
     4321 gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag ttctacatct
     4381 ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt ccgtaacatc
     4441 aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat ccctatagaa
     4501 gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag tcccaattgt
     4561 gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa tggattattc
     4621 gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc tggcatttgt
     4681 ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc aagtagctgc
     4741 tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa tgcaagaatc
     4801 atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga ttctcctaaa
     4861 gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat gatcagagta
     4921 agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa acttgccacg
     4981 ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa gctaaatgta
     5041 tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc tggatcagat
     5101 agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag ttcatttgca
     5161 aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt catacccata
     5221 ctgccgcttg tttcgctgga ttcataaatt cctttgatcg tcatttcata agtggtatct
     5281 tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt tgtttctgct
     5341 agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat tccttcacca
     5401 tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc tgacacacca
     5461 ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc agcaggttca
     5521 ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt gatggcagtt
     5581 attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt tacattatct
     5641 aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa actgcctgga
     5701 tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt tttaaatgaa
     5761 gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc ttgattcgct
     5821 gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag aatggctgtg
     5881 aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt ccatgcacgt
     5941 ttcataaaat tcatattttg catccctcct agtagatgca tttgataata gacaacctat
     6001 cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt tctgtatata
     6061 taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta tgtttcaaac
     6121 cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac agagggattt
     6181 ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat ttgttgtata
     6241 ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt cgcaaaagat
     6301 ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca gaacgcggat
     6361 gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt tgcatatgct
     6421 tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta aacttggatg
     6481 tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg cggcgttcgt
     6541 gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat tttagattgt
     6601 gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc tgattttacg
     6661 tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg acatgcagat
     6721 gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt cgggacagag
     6781 ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa aggatttaag
     6841 gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt agtgagcttt
     6901 ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac aaaagaaggt
     6961 agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat gtatcaaaca
     7021 gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact aagtgaatcg
     7081 gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga tgaagtattt
     7141 gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag taatttgcaa
     7201 cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt cttgactggt
     7261 caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca attaccaagt
     7321 gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga actgatcatt
     7381 ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc aaacggtaca
     7441 caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac gccaagcatc
     7501 gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg tggaacagtg
     7561 aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga cttgcaatta
     7621 atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta tcgtgaatta
     7681 gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat ctatattact
     7741 ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg aactacaagt
     7801 gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat tgttttacgt
     7861 gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat cagtcggcga
     7921 gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta cgtaaaagct
     7981 agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga gaaacattta
     8041 gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca attaggccgt
     8101 tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat ggaagctact
     8161 caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata gagtgctcgt
     8221 cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc gctttataat
     8281 aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga acgttattta
     8341 gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga agaatctttc
     8401 acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac tctcgtagtg
     8461 aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca cttagattac
     8521 aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat ggattttgta
     8581 ttaatgcata caggatatcc ccacggagca aacaccccta ttggaataaa actcgcacat
     8641 cctgattatt gttttttagc agacagttct ttaaaagaaa aagaagaagt aattgtgtca
     8701 agcggtgaaa tcgggagaag ccttcggata aaagtagccg atttagaaaa gatcgtccat
     8761 cctatttacg ctgatttaat tagttaaggg acgagaagag aagctaagca gagcagagac
     8821 ctcaaacttg agattatatc tgcttggtac ttggtgcatc ctgtattatc tgttacaatt
     8881 gctttgtaca tactgtacta tcaatccaat gaaaatatat ttagcagcta tattttttca
     8941 ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa cgcgaggtgt
     9001 aaaatatgaa aattacgtat catggacatt cctgtttaac aattgaaatg gaaaatggcc
     9061 aacgcgtgat gattgacccg tttattacag gaaatccatt gacagactta actgcagaag
     9121 aagtaaagac agattggttg ctgattactc atggtcatag tgaccacata ggagatatga
     9181 ttcctattgc aaaagaaaat caagccacaa ttatcagtat tgttgagatt gctgattatg
     9241 cgaacagcag aggagtagat tcattcggta tgaatatcgg tggaaaacat gcgtttccat
     9301 tcggcgtggt aaaattcgta catgcacagc atagttcaag ctatgaagta gacggtatcg
     9361 ttcagtatat gggcgagcct agcggaatta ttattcaagc agaaggaaaa acgatttatc
     9421 atgcagggga tactgcttat ttcagtgatc taggattgct tgcagaagaa ttcgatattg
     9481 atgtcgcttt cttgccgatt ggcgataact atacgatggg gccagaagat gcagcaaagg
     9541 ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca ttacaatact ttccctgtca
     9601 ttgaacaaga tcccgaatct tttgtagagt tactggaaga tagtgaagga aaaataatga
     9661 gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga caaaacatga tttgatttta
     9721 gaatatattg aaagtttacc tgttggcaat cgaatttctg taagaagcat cgcaaaagaa
     9781 ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa tgtcggacta
     9841 gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa aaaacatatt
     9901 gaacgtttga catttggcga ggtcgtcagg atcattgaag gggacgtttt aggcggggcg
     9961 gcaggattag acaaggtctt gaataaattc gtcatcggcg cgatgactaa acatgccatg
    10021 gaacgctata ttacgccagg atcactaatg atcgtcggca accgggcgga agtccaaaaa
    10081 ttagcattgg aagatggcgc cgcagtattg atcacaggag gattcaatac gactgctgag
    10141 attgcaaagc tagctgatga tcttgaaatg ccgatcttac gcacctctta tgatacattt
    10201 acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa agatatcatg
    10261 cttgtcagtg atatctatat gccagctgaa aagactcatt atttgcagca gacagatacg
    10321 atttctgatt atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc agtcgtcaac
    10381 cgccatcatc gtttgatggg gattgttacg gcaaaagatg ttttagggaa atcgccaaac
    10441 cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac aatgagtatt
    10501 gcctctgtta gccatcaaat gatctgggat gggctagaag tcatgccggt cgtttccgat
    10561 gacttgacat tggaaggctt gattacccgg caagatgtaa tgaaagcgat gcagcttgtc
    10621 cagcgacaac cgcagattgc agatacgatt tcggatcaga tatctggaaa tatccatacg
    10681 attgatactg accgtgaagg aaatcgtttg gaaaacccta agttcaaatt cgtagtagca
    10741 ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg tacttagcga aatcatttct
    10801 gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat tgaacaagtg
    10861 aacctacatt atttgcggct gatccaattg gaaagcgaat tagacattcg tccacgtgta
    10921 ttggaaatcg gcagacgttc cgctaaactg gatatcgaag tgtttattga aaacaccatc
    10981 gtttccaaag caatcgttgt ttgtcaagta atggaacgtt cttagaaagc aggagtgaaa
    11041 tgacagtaca gcaagaaata gtagatacaa taaaaaaata cgataaaatc attatccaca
    11101 ggcatttgcg ccctgatcct gatgcattag gatcacaagt aggattagcc gaactattac
    11161 gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa gggcttcgct
    11221 ttttagcaga aatgcaagaa gttgaagatg tcttctacca aggagcgcta gtcatcgtga
    11281 ctgatacagc taattcaccc agaatcagtg atcaaagata tcagttggca gaaaaactga
    11341 tcaaaatcga ccatcatccc aatgatgacc cttatggaga tcttgtgtgg gtaaatacaa
    11401 aagcaagtag ctgtagtgag atgattgcag aatttgcttt gatgtttcct gaatctttaa
    11461 agatgaacgt taaggctgca cggttacttt atgcaggaat cgtcggagat acagggcgct
    11521 ttttgtatcc ctccacgacg tcccgtacat tagagattgc ttcgatattg agaaattatc
    11581 catttgatgc aagtgctttg aacagagaga ttgaacaaat gccgatgaaa gtcgctaaac
    11641 tttccggtta tctttatcaa aatatccagg tggatgaaaa tggtgcagga agaataatcc
    11701 ttccgcaaga aattcttgat aaatatggaa tcgatgattc agagacagct tcgatcgttt
    11761 cacttcctgg agtgattgac gaagtgctgg cttgggggat ttttgttcag cagccttcag
    11821 ggtattaccg cgtacgttta cgttctaagg ggccgatcat caatgaattg gctaaacgcc
    11881 atcatggagg aggccatcca ttagcaagtg gtgcgaatgc aaaagattta gcagaagtag
    11941 aggaaatcta taaagagatc caagatatat gccaatcgta taagagcaga aaaacagaat
    12001 gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc ttttcaagat
    12061 cttctttttc cgttacaata gtgctcacta aagattttaa aggagaaaga agatgacatt
    12121 accaaattgc ccagaatgtg gctcagagta cacatatgaa gatcgaggct tgttgatttg
    12181 cccagaatgc gggaacgaat ggaacaaaga agaacaagaa actgtcatag aagacggatt
    12241 agtggtatta gatgcaaatg gaaaccgtct gttagagggc gatagcgtga cagtgattaa
    12301 agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag tcaagaacat
    12361 ccgtttagta gaaggcgatc ataatatcga ttgcaaggta gaaggatttg gaccgatgaa
    12421 actgaaatcg gaatttgtga aaaagaatta aagaatattc aaggtctttt cacattcttt
    12481 tcctactact aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa tgtatggtta
    12541 tggaatgtat ggttttttag atccgacatt tcttttggtc attcttggat tagttatttc
    12601 cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag tgagaagcaa
    12661 aaacaatgtg actggtacac aagcagcaca atatatatta caaagtcaac agataaataa
    12721 tgtcggtgtt caacaaattg ccggagatct cacagataac tacaattcag gtaataagat
    12781 gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag gtgtagcagc
    12841 tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat gtcccattga agattcgtgc
    12901 agcacttgtt ccagcagcta actttggttc gatgatttcg ttccccttga ttatggtagg
    12961 tgtattgctt agctggaact ctacgttaat caatatcggg attttcgctt tttctttagc
    13021 attacttttc caattggtga cattaccagt cgaatttaac gcgtctcggc gagcaatcca
    13081 aattttgagt gaaggcggac tactgacgga agaagaagta ccgatggcaa gacacgtatt
    13141 atttgcagca gcactgactt atgttgcagc agcgctttcg acattcctgc agctgttgcg
    13201 cttgatctta ttgttcggcg gcaaccgaag agattaaaat aaagaagttg tgaaatcagc
    13261 gctttgatct gagctaatac cgagagttag ctgattgaat accatttatt tggaataaga
    13321 ggatgtgaca agaattttgt cacatcctct ttgttttgtt ctcataacct tttttcatat
    13381 acagttcatg aaccgctttt attaacttgc caaataacac tgtattgtgc gttgtatcca
    13441 gatagaaagt ctccgtttca tctgaaattt tctcatgctg atctgtaatg atcaaatcag
    13501 catgttctga atatgcggta attttaacag catctttgcg aaataactgc tgcaaatggc
    13561 tttgaagatg ataatttcct gtaaactgtt tggttatctg caaatggatg agtaactgct
    13621 ctttcgtttc tgtttgtagt aagtgttgga tcaattgact aaggtattga ctgatttggt
    13681 ttctttcccc attttttgaa aaaattgtat cgattttttc tcttaatttc ttttcctgta
    13741 taaaagaact agaatgattg gacgtggctt ctggaaagca aagtgtagaa aaactactgc
    13801 tggattcacc caacagttcg aaaaaaacaa ctgccaacac aagaaaataa aaataaattg
    13861 gtattttatt ttctaaatgg ttgagaggaa actgttgagt taagtaagtc atcaattgac
    13921 cgacttgtcg acaaataagg tttttgcttg tggaaaataa aaaaccaatc tcctgttttt
    13981 gtcgaaaaga gatttgattt ggaaaaaaac aatagaaaaa
//
LOCUS       DAFCNS010000070        14016 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFCNS010000070 DAFCNS010000000
VERSION     DAFCNS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.072"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(538..900)
                     /locus_tag="LNE23_002161"
     CDS             complement(538..900)
                     /locus_tag="LNE23_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6931994.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(974..1354)
                     /locus_tag="LNE23_002162"
     CDS             complement(974..1354)
                     /locus_tag="LNE23_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBK6931995.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1418..2611
                     /locus_tag="LNE23_002163"
     CDS             1418..2611
                     /locus_tag="LNE23_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6931996.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2688..3272)
                     /locus_tag="LNE23_002164"
     CDS             complement(2688..3272)
                     /locus_tag="LNE23_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6931997.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3473..4471
                     /locus_tag="LNE23_002165"
     CDS             3473..4471
                     /locus_tag="LNE23_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6931998.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4583..5092
                     /locus_tag="LNE23_002166"
     CDS             4583..5092
                     /locus_tag="LNE23_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBK6931999.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5122..5781)
                     /locus_tag="LNE23_002167"
     CDS             complement(5122..5781)
                     /locus_tag="LNE23_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBK6932000.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5783..6547)
                     /locus_tag="LNE23_002168"
     CDS             complement(5783..6547)
                     /locus_tag="LNE23_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBK6932001.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6555..7232)
                     /locus_tag="LNE23_002169"
     CDS             complement(6555..7232)
                     /locus_tag="LNE23_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBK6932002.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7269..8654)
                     /locus_tag="LNE23_002170"
     CDS             complement(7269..8654)
                     /locus_tag="LNE23_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK6932003.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8864..8948)
                     /gene="ffs"
                     /locus_tag="LNE23_002171"
     ncRNA           complement(8864..8948)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LNE23_002171"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8971..9486)
                     /gene="tadA"
                     /locus_tag="LNE23_002172"
     CDS             complement(8971..9486)
                     /gene="tadA"
                     /locus_tag="LNE23_002172"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBK6932004.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9593..10450
                     /locus_tag="LNE23_002173"
     CDS             9593..10450
                     /locus_tag="LNE23_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6932005.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10531..10866)
                     /locus_tag="LNE23_002174"
     CDS             complement(10531..10866)
                     /locus_tag="LNE23_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932006.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10933..12303)
                     /locus_tag="LNE23_002175"
     CDS             complement(10933..12303)
                     /locus_tag="LNE23_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBK6932007.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12381..12917)
                     /locus_tag="LNE23_002176"
     CDS             complement(12381..12917)
                     /locus_tag="LNE23_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6932008.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13226..>14016
                     /locus_tag="LNE23_002177"
     CDS             13226..>14016
                     /locus_tag="LNE23_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6932009.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERA"
BASE COUNT         4277 a         2798 c         2281 g         4660 t
ORIGIN      
        1 cccgattcgt ttttggctgc cgtagcaact tttatatcat atcagagaga caagtaattg
       61 tcaactcttt tttaaaagtt tttttgattt cgtttttcga aatctttcag caactcattt
      121 atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt cgatagaatc
      181 aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtcg ttagtaacat
      241 gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt cagaatcaac
      301 gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca ttactttgtg
      361 aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac atattttgaa
      421 aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt ttggaccaaa
      481 caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat ctgtatttta
      541 gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt gacctggttc
      601 ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag tttcaaagac
      661 aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca aactaatctt
      721 gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta aaccagtctt
      781 tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat ggaccaaaaa
      841 ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt tgcttgccaa
      901 aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca cattttcaga
      961 aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat gcttcttcta
     1021 tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt ttattcgtga
     1081 aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt cttaaagata
     1141 aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact ggctgtccga
     1201 cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa acatgaataa
     1261 gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc cctgtcactt
     1321 ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc gcatttatca
     1381 ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc ttaagatgat
     1441 cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg acgcaattgc
     1501 tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg acttcttgtc
     1561 tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct ttggttatgc
     1621 cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac accatgctgt
     1681 ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt tagctgcagc
     1741 agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag tttatcctcc
     1801 attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt tgctagaaaa
     1861 aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag aaaacaatgt
     1921 aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga gcaaagaaga
     1981 attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta gtgacgagat
     2041 ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga ttgatcctaa
     2101 tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc tagctgcaat
     2161 caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg aaaaacattt
     2221 agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc aagcggctta
     2281 tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa atgttcaaac
     2341 agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc ctgaaggaac
     2401 ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat tggagaacac
     2461 tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc cttccggtca
     2521 tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag gacttaatcg
     2581 aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa aggacgagac
     2641 cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta ttctgaaatt
     2701 tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg ttgtacattt
     2761 gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt atgtcggaaa
     2821 tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg gaatccagca
     2881 ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat aaagaattgg
     2941 cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag gttgaacagt
     3001 tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc cattcctgtt
     3061 ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa aatcacgcca
     3121 tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg tgctagttca
     3181 gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt tggtcctttt
     3241 tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat ttgcatactt
     3301 cataataata ataacataat acctaacctc tcgtaaaaat actagatttg ttttctttta
     3361 tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc aaacattttt
     3421 gagaagcatt ctcaattaaa ttctgatata gtgtgaaaag ggaggaattt ttatggaaat
     3481 ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt tttacgcagg
     3541 aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg gtcaattagc
     3601 aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa taaaagcaag
     3661 tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat tccatttaaa
     3721 agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta caaataaagg
     3781 catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc agccaagaaa
     3841 attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt tcgtgaatga
     3901 tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt ctgctatcga
     3961 ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc gtcgcccgga
     4021 atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca acttgcttac
     4081 tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata tccggttaaa
     4141 aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg tcaattatgg
     4201 attctcttct aatttagaac atctttcttc ttggggactt gatagtacca gaaatgccat
     4261 tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg gtgacatctg
     4321 tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac ctactgcagt
     4381 gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc acagcacaag
     4441 cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca tttcaagtta
     4501 aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat agagaaagaa
     4561 aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag aaaaggcaag
     4621 agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg tcatgttttt
     4681 acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg tcaacagcgt
     4741 gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag atatattagc
     4801 agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg aaggccttta
     4861 cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga tcggctttac
     4921 gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa acagccacga
     4981 tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg ctgctgctgc
     5041 cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat aattcaacaa
     5101 aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt tcaagctatt
     5161 ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa ataagataaa
     5221 gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa cgggatcgaa
     5281 taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc gatcaaatcc
     5341 tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa acggacgaat
     5401 caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac tcgggatcaa
     5461 agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat aaaagtgatg
     5521 ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga agggattatt
     5581 gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta aagaagtata
     5641 atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt tgatcgtcac
     5701 accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat attccatcca
     5761 ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt ttcacatagg
     5821 tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat aaaacgagca
     5881 aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta tctccaacca
     5941 tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata atgatggctt
     6001 ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa gagatgactg
     6061 accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg ttcgttccaa
     6121 tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca taggtcagat
     6181 aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta aatccggcag
     6241 ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc ccttttccat
     6301 ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct ggagctacat
     6361 gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta gtcgtgttat
     6421 tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat cgtttaccgg
     6481 ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa tatcctttat
     6541 agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt tgttttttct
     6601 acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga ctctgttttt
     6661 cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt ctcatcgata
     6721 tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc ttttttaccg
     6781 ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta aagatagtct
     6841 tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg gaaaaatcct
     6901 tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg ggctagtaca
     6961 tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc ggacacaagc
     7021 cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac tagctcgcct
     7081 ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt cagttcttcg
     7141 gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt tttatgctca
     7201 acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt ttttattata
     7261 ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac acttcggata
     7321 aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa aaatggaaca
     7381 aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat tggcttgttc
     7441 ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac catctgccca
     7501 aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa gttacgattt
     7561 ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac atttaacaag
     7621 tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc ttttggaatt
     7681 tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg ttctttcacc
     7741 gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact cagagaaaat
     7801 ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag ataactattt
     7861 atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc tttggctgaa
     7921 tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac atactgcccg
     7981 tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct gaataaatga
     8041 tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat ttggaaatca
     8101 tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg taaatcttcg
     8161 actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg attataagta
     8221 agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt cgttgtaatg
     8281 attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa taagttatcc
     8341 aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt ggaatttcct
     8401 acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat cagcaagata
     8461 ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa atcacctaga
     8521 tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg ttgatccaaa
     8581 aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt atgtaaaatc
     8641 actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat aggcagcttt
     8701 ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca aattgtataa
     8761 gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt ttacagtact
     8821 ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat cagacacatg
     8881 gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag cccaccattg
     8941 tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc agtctttttt
     9001 taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca tcccacattc
     9061 gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt cctccaaaag
     9121 attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat aaacttctgc
     9181 tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg taacaaaaag
     9241 tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga ttgcaagcat
     9301 ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc ttccaatcac
     9361 ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt ctgctttctt
     9421 tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa gatttgtatt
     9481 taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata tgataaaaag
     9541 gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca agatgaaaat
     9601 ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag catcagaagc
     9661 ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag attcattatc
     9721 tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc aaaaaaaaca
     9781 cccatggtat cactatcttt ttaaagatgc ttcttgtgtt caagaaaaaa gctttcgttt
     9841 gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc aagatactat
     9901 tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag atgcgctctt
     9961 agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta tttttctatc
    10021 tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc attcacctgg
    10081 caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag agcaaaacaa
    10141 tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt actatacaaa
    10201 agatactgta aaagatagta agttgatcaa aagttacata gaactgatca aacaaaatga
    10261 aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct ctgctgcaaa
    10321 aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc aagaacagtc
    10381 tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat tgcttcatga
    10441 acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac cccttatttc
    10501 gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa aatgcattag
    10561 ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag agattttctt
    10621 ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac tatctgaaag
    10681 accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga cgaatttttt
    10741 tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg atacaacgac
    10801 tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt ttgttgatgt
    10861 tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa aaaaataaac
    10921 gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta ctgttccttt
    10981 tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc atccttgatt
    11041 cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt gagtgatcgg
    11101 caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc taggttcaaa
    11161 tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta aaccatcgag
    11221 agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa gtcttccagt
    11281 agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct cttttgtcat
    11341 ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt gtccatgaaa
    11401 agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata tacgtattgt
    11461 tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg attgagtcaa
    11521 aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga tctcttcatt
    11581 ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt ttagcatttt
    11641 tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag attcttctgt
    11701 ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac aaggagcatc
    11761 aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg gtgcatgatt
    11821 tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa tcttcgcacg
    11881 tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag cagctaattg
    11941 ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg gttttgctgc
    12001 agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc cagcttgatg
    12061 gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg gagcgggttg
    12121 taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta gtggattcca
    12181 ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg atgcttcttc
    12241 gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg cttcctcctt
    12301 cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa ggtggctgat
    12361 ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc caagtattca
    12421 tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc ttgagaagaa
    12481 tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga cttttcttct
    12541 ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag cgaatccatg
    12601 tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa aaaatctttc
    12661 aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta gttttcaaaa
    12721 taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt gaaatgtgta
    12781 taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt aattgacttc
    12841 attccatatt cttccacgta taccatactt ttgaacagca aatattcttt actataattt
    12901 gttactctcc ccatcattct tacctccgtc tcttttcatc tacccataaa taaataacat
    12961 aaataattct aaaaatatta ttgataatta tcaaaacata tttagttata tccattaaaa
    13021 atcaattttt ccgccttaaa aacaagaata tcttgacaaa ataccctagg aaataaacaa
    13081 tagaataaac aaggtaacaa aaaaattaaa taacgttata taaaaaagaa ggcgacactc
    13141 tgtgagaata acacagactg aaatgacttt ccactcacag tttttgtcta actatataca
    13201 aaaacagaat aaaaaggagg tatttatgct aattgaagca agaaaaaaag agttacttgt
    13261 tctacaaatg gtcattcaaa aaaataattg ttctttacaa gaattatcca atgacttaca
    13321 tattccaaaa cggacaatca aagaactgat ccgaaaaata aatgtatcac tcaagcaatt
    13381 tttattgatt gatgaattta tttattctaa tcataaagga gagatccaaa ccaaagaaac
    13441 cgatcaaact aaaaagttag ctgttttttc taagttaaaa ttatgctacc taaaagaatc
    13501 caatcgtttt aactatttga tgttactaat caattatcca caaactgctg taccaaaaaa
    13561 atatttactt gaacaacttt atatatcgcc ctcctatctc gaaaaactga caaaacagct
    13621 caatcattcc ttaataaaat tccatttacg gatcctatct gtaaaaaatt gctgtactct
    13681 tgaaggaaat gagttatcta tccgtctcta tctttacttt tttctttcag atacatttgg
    13741 aggtaacgat tggccatttt cctcacttga attaaatgca ctaaaaatga acagtacaga
    13801 tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc tacttgctgt taactttatt
    13861 catattacga aacaaacaac aggcatattt tccaaaaact tcttctgaaa tcactgaatt
    13921 gatggactta ttgcagcata cacatgatta ttcagaatac tttacttctt tttcagtgag
    13981 tcgcccttgt gaagagacag aaacagagag agccta
//
LOCUS       DAFCNS010000071        13839 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFCNS010000071 DAFCNS010000000
VERSION     DAFCNS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.073"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(184..2181)
                     /gene="tkt"
                     /locus_tag="LNE23_002178"
     CDS             complement(184..2181)
                     /gene="tkt"
                     /locus_tag="LNE23_002178"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBK6932010.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2283..2534)
                     /locus_tag="LNE23_002179"
     CDS             complement(2283..2534)
                     /locus_tag="LNE23_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBK6932011.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2681..3304
                     /gene="lexA"
                     /locus_tag="LNE23_002180"
     CDS             2681..3304
                     /gene="lexA"
                     /locus_tag="LNE23_002180"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBK6932012.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3484..3702
                     /locus_tag="LNE23_002181"
     CDS             3484..3702
                     /locus_tag="LNE23_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932013.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3991..4503)
                     /locus_tag="LNE23_002182"
     CDS             complement(3991..4503)
                     /locus_tag="LNE23_002182"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBK6932014.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4517..6814)
                     /gene="recJ"
                     /locus_tag="LNE23_002183"
     CDS             complement(4517..6814)
                     /gene="recJ"
                     /locus_tag="LNE23_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBK6932015.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6956..7399)
                     /locus_tag="LNE23_002184"
     CDS             complement(6956..7399)
                     /locus_tag="LNE23_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBK6932016.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7400..8188)
                     /locus_tag="LNE23_002185"
     CDS             complement(7400..8188)
                     /locus_tag="LNE23_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6932017.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8185..9126)
                     /locus_tag="LNE23_002186"
     CDS             complement(8185..9126)
                     /locus_tag="LNE23_002186"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBK6932018.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9303..9890)
                     /locus_tag="LNE23_002187"
     CDS             complement(9303..9890)
                     /locus_tag="LNE23_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBK6932019.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10111..11418)
                     /gene="obgE"
                     /locus_tag="LNE23_002188"
     CDS             complement(10111..11418)
                     /gene="obgE"
                     /locus_tag="LNE23_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBK6932020.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11721..12731
                     /gene="gap"
                     /locus_tag="LNE23_002189"
     CDS             11721..12731
                     /gene="gap"
                     /locus_tag="LNE23_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK6932021.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12816..13751)
                     /locus_tag="LNE23_002190"
     CDS             complement(12816..13751)
                     /locus_tag="LNE23_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6932022.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3819 a         3098 c         2438 g         4484 t
ORIGIN      
        1 gcttaatttg acaaataata ttctcaagaa acacaaacta atccgaagaa ttctttgttt
       61 tgtcatttaa atgtaacata gccgaaaacg aaaagtaata ttgatttatt cttctaacac
      121 ataaagaaca gctgtctcca acttgcagac agctgttctt atcagtcaaa caattgcttg
      181 gatctatgat agttgattgt acgtattcac gacattttct actgtaaatc caaattcttc
      241 taaaatttta gtgccagggg cagatgcacc gaaatgatcg attgtaatcg ttgcgccctc
      301 tgttcctacg tagcgttccc atccaaatga tgcagcagct tcgatagcca cacgtttctt
      361 cactgattta ggaagtacac tttctttata ttcagaagat tgtttttcaa agaggtcaaa
      421 gcttggcata gaaacaacag aaacatcttt gcctttttca gccaattctt tttgagcttt
      481 cacagccaaa tcaacttcag aacctgttgc aatcaaaata ccttctggtg tttctccttg
      541 tgatggagaa agaacatacg cgcctttttt caccatttca tctgctactt cttttgtaga
      601 aggcaatact ggaaggtttt gacgactcaa tacaaggata gtaggcgcat ctgttgtctc
      661 catcgctact ttccaagcag cacgtgtttc atttccgtct gccggacgaa tcacttgaac
      721 accaggcatg cagcgaacag atgctagttg ttcaatcggt tcgtgcgttg gtccgtcttc
      781 tccgacagca accgagtcat gtgttaagac gtaaacaaca ggagtatgtt ggattgctgc
      841 taaacgaact gctggtctta gataatcgac aaagacaaag aatgttccac cgtagatacg
      901 agttccgcca tgaagttgga taccattcat agccgaagcc attgcaaatt cgcggacacc
      961 gaaccagatg ttgcgtcctt cataatgttc aggagtgaaa tctttatctg cagccaccat
     1021 tgtattattg gaacctgaca agtccgctga accaccccag aagcttggta tagctttcga
     1081 taattcttgg atgacttctt tacttgaaac acggcttgct tgactgcttc caacttcata
     1141 agttggcaat tctgcatccc agttttctgg tagtttgcca tcaaaagcat cttcaaattg
     1201 ttgtgctaat tcaggataag cttttttata attggcaaac atttcattcc atgcatcttc
     1261 agctttttgt ccttcttcta tcatcgtttg atggaagcgg gcagctactt cttctggaac
     1321 agtgaaatct ggatactccc aaccataaac ttctttagcc atcttgatgc catcttcacc
     1381 aagaggagca ccatgaacag cagatgttcc ttctttagga gcaccatagc cgatgactgt
     1441 ttttacttcg atcaatgttg gtttgtctgt ttcagctttt gcttcttcaa tcgctttaga
     1501 gattgcttct aggtcatttc cgtctttcac taagatatgt tgccagccat atgcttcata
     1561 gcgagcacca acgttttctg tgaaagcttt tgaagttgga ccgtctagtg aaatatcgtt
     1621 tgaatcgtat aaaacgatca atttccctag tttcatatga cctgccatcg aacttgcttc
     1681 ttgagatacc ccttccatca agtctccgtc accgcatatt gcatacgtat agtgatccat
     1741 cacattaaag ttttctttgt tatacgttgc tgcaaggtgc gcttctgcca ttgccatacc
     1801 gacagccatt gcaatccctt gtcctaatgg ccctgtagtg gcttctacac catctgtatg
     1861 atgtacttca ggatgtcctg gtgttttaga atcccattga cggaattgtt tcaaatcatc
     1921 gattgtcact tgatagccag ctaaatgaag caagctatac aacattgctg acccatgtcc
     1981 tgccgaaaga acgaaacggt cgcggtcagc ccagtttttt gatgtcgttg gatttacttt
     2041 caggtgtttt gtccataacg catatgccat aggtgctgcg cccatcggca atccaggatg
     2101 tcctgagttt gctttttgga ctgcttcaat acttaatgta cgaattgtgt taacgccaag
     2161 ttggtcaatt ttgtcaaaca aaataacccc tcctattaat ttaggttttt tttaccttta
     2221 tttcttacac tttcattcta taagatgtca cacgaaaaag caatcttttt cagtattttt
     2281 tactaaaagt tgtcatttcg gttgtgtaag ccttttttct tttggatttc ttttaatttt
     2341 tccggggtca cgtcattgcc ctcaggatcg acgactttca ttccttcgat atgatgacgc
     2401 ataccggaac gaaaagcttc caaatactct ttgcgcaagt tgatttgttc ttgttcttcg
     2461 gcttttgaga gaccattttc cttcttttta cgtgcaagtt cattgatccg ctctattttt
     2521 tcaggtgaca gcatgctcag ccctcctttt actctccttc atattatata aaaactgaaa
     2581 aaaaaacaac tagataaact aaataaaata cgaacattcg tttgatttag cgctttttat
     2641 atggtactat ttatttatca agtgaaagaa ggtgtacctt gtggcccgac aaactgaatc
     2701 aagacaaatc gaagtcttac gatatatcca tgaacaagta acccaaaaag gatacccgcc
     2761 gactgtccgt gaaatcgggg aagctgttca gctttcttct acttctactg ttcacggtca
     2821 tcttgcacgg cttgaaaaaa aagggtttat ccaaagagat ccgacgaaac ctcgtgcaat
     2881 cgagttgaca aaacaaggtt tagaaaagat tgggattcgt ccaacaacta ttccagtact
     2941 tggcgtcgta acagctggag aacccatttt agcagtagag gatgcttcag acttctttcc
     3001 aatccctcct aatctttctt ccgaagaagg cagtctcttt atgctgacaa ttcgtggaga
     3061 tagtatgatc aatgctggga tattggatgg tgacaacgtg atcgttcgga agcaagaaac
     3121 tgctcaaaat ggcgatattg tcattgcaat gactgccgag gacgaagcga cttgcaaacg
     3181 attctataaa gaaaaaaact ttttccgtct gcagcctgaa aacgacttgc tcgaaccaat
     3241 catcttagat caagtatcta tattaggacg agtagtagga ctttatcgta gtcatatcta
     3301 ttaattgcta ctaattaaaa gaaaactttt taaaaagagt ttgagacgtt ggtctcaaac
     3361 tcttttttcg cccgggttct tcttctttca aatttttatc acttccctct ttacaaaccg
     3421 aacaaatatt cgtatactaa atatacgaac aattgttcgc ataaattaaa gaggtgagaa
     3481 ttcatgaaat caatgagacg tcttggctta gtattaactg ttttgttttt aggtattatt
     3541 attggtaatt tcggcatacg tccagcttta cttgtgtttg ttccgttatt tactttgtgg
     3601 ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc ggcgcagaat tcatgaagat
     3661 catttctatc atgagcccta ctacagatcc aggcaatcat aataaggatt gactgctaat
     3721 tattcaaaag acaagaagtt aaacttttac gggggtgtga caaaaatcgg tcacatcctt
     3781 tttttcgaat aaacggtgtt ctaaagccag tttttcggta ttaagtcaaa ttgcggaaaa
     3841 agtgattttt taaacttcta ctggtctttg cttgtttcca tttataaaaa aatagcgacc
     3901 ctcaaaagtc agatagagaa tctgactttt gagggtcgct ttaaaatcgc ttctttaaga
     3961 ataacatcaa gaatagcttc ttttttctgc ttaatattcc attaatgtaa tgatgtcata
     4021 tcctttgatt ttgtcgcgtc catggagatc catcaattcg atcaaaaatg cgcagcctac
     4081 gactacaccg cctaattgtt cgatcagttc gatcgtcgct ttgattgttc ctccagtagc
     4141 tagcaaatcg tcacaaatca gcacacgttg acctggttta atggcatcct tatgcaatgt
     4201 caatgtatct gttccatatt ctaatccata agtcacttcg atggtttctc gaggtaactt
     4261 cccttttttg cggactgggg caaaaccgac tcctaactca taagcaacag gacatcctac
     4321 gatgaatcca cgggcttctg gcccaactac catatcaatt cgtttttctt ttgcataatt
     4381 tacgatttgt ttagtagctt cccgataagc gtcaccatct gccatcagtg gggaaatatc
     4441 tcgaaacaca attccttttg acggatagtc gggaatactg gcaatataat cttttaaatc
     4501 cactctttgt ttcctcctag gttgttaacc atttctttat ggtaggaata tcgctcaaaa
     4561 gcaaaaattc ctcaactttg attttattga gccgtcgctg gtataatctg ctctcagtca
     4621 aaggatgact ttctgggcta gcgacttttt gtaaaacacc gttctctatt gtaacaaatt
     4681 tcaattcaaa aaacacctgt atcataaaaa ttaataattt ttcagggata cgcaaatatt
     4741 gagctattgc ttttatttta tgacgtatat caattcgttc ttgagaatgg atcaatttaa
     4801 acaattttgc gtactgttct cttgtgccta ccccattcaa ataggcctca tcaggtgaaa
     4861 tccccatcag ataaacacga ttgaatgaat aagaccgaaa aacttctttc agatccgcta
     4921 aatcatctgg acaatccata acaactagag agtttggttt tcttttttca attattgcct
     4981 ttacagaatc tagtccttcg aaaacaatcg cagcttcctg gataatctca ggcacgaatt
     5041 tcaacgtcga agaatcaaat gctaaataaa ggctaccttc cgacgttcgg ctctgttcac
     5101 ggaaacgttt tgcccgccaa tcaaatactt gcagaccacc cacagaaaaa tcagatacca
     5161 tcagctgcgg tttcttacgc ccgttccatt cattgatcga aagctttcct accacatcta
     5221 cctgattatt cagcagttct tcttcttgag taccaaaacc aaaagcgacc gcatcaagct
     5281 gactcgatgc atcggaaagg acaaatttca gatgctgttg gtttgctccg attcttttga
     5341 tattttctgc ttggacttgt cgaaataaaa actgaggcaa aggattatcc gtaccaaatg
     5401 gcgccaatag tttcagctca tcaattcgct ctactgttac ttcatttggc agcaatactt
     5461 catcaattct caactcaggc cctctcatca agtcaatttg atgatcgact atatattggt
     5521 tcatcttctc ctgtagtata gtgacatttt cacttggcat cgttaaaccg actgctgcat
     5581 gatgtcctcc gaagtaagta aataaatctc gcatctggtc aagcatttca aaaagattca
     5641 aggcttcaat actacgtcca gatcctttag ctgatccatc ttcttttaaa gtaaggacaa
     5701 gcgttggttt acccgtttca ttcattattt tcccagcaac aatgcccaaa acgccttcat
     5761 gccatcctcg gtttaccaat agatggatat gattctcctt attgaccatc gctaatgctt
     5821 cttgtgttat ctgctctacg attgcttttc tttcttcatt gatctcattc aatttttttg
     5881 cttgaacgga tgcttcctct tcatcaaacg tcgcaagtaa cgagacggca ggacgtggat
     5941 cgcccatccg accgattgca tttagtctgg gagcaatcga aaaaccgatc gttgtttcgt
     6001 ctgcgtccct catttttacg ccgctttctt caaatagcgc ctgaagtcct atccgttctg
     6061 aatgatgaat ggcatcgatt cctaatgaaa caaggattcg attctcatca gtcaatgaaa
     6121 ctaaatcggc gatcgttcct atcgctacta aatccaaaaa ttctgcaggc ggttcttcca
     6181 ataatgcaga agctactttg aatgctactc cgactcctgc caaatctgga aatggatagt
     6241 gccccgctgg atgacgaggg tgaacaatcg cataagcgtc tggtaaattc tcaggtagtt
     6301 cgtgatgatc tgtaatgatc acgtctacac cagcttgctg agcgtatgct accgcatcat
     6361 ttcctgctac cccgttatca acagtgatga tcaactggat tccttcttcg atcttttctt
     6421 ggtataccga ttggtttggt ccatacccat gctcaaatcg atttggcagg aatacttcta
     6481 cttctgcacc aagtaattcc agtgtttctt tcattaccgt tgcactggtg atcccatctg
     6541 cgtcataatc accgtaaacg aggatttttt ctccactgat tacagcttct tggattcggc
     6601 tgattgcttt atccatatcg tgtatcagat acggatcatg taattcttgt tcctttggat
     6661 gtaaaaatcg gttgatatcc ttttcctcat ggtaacctct ttgccacagt aacttaccga
     6721 caaatggaga taatttcagt tcacggatca tttggagaaa ggattcagaa ggctctgtct
     6781 cgcttaactt ccaatcaaaa tttgcttgtc tcactttctc actcctaact tagttattat
     6841 agcaaaggaa atcagcaaaa aaaagcagaa agaaagaata aaaacagatc agatccgcta
     6901 gacagatgtt cagcaggacc tgatcttttg tacgaccatt tatgaaatat ccctattagt
     6961 caaaataatt gaagctttgt ttgggcgatt gagttttttg ttcttccggc gctttcaatt
     7021 gactgatttg tgcggttttc gcttgtaact ccgcctgata agcagcttcc aactccgcac
     7081 gttcgtttct aaactcgcgt tgctgttctt cttttgcttc ttcaattttc ttttgaagtt
     7141 ctgtttgata ttctgacatt tcctgagtca gctgtttgat ttgctttttt tgctgccata
     7201 gtgttgccga agcagtcaaa aatacaatca gtgcaccaat cagcgcagaa ccgatgatca
     7261 ctaagatcaa tggcgcagaa aaggaagtga agccaaaact tacaggaaca tttttattgt
     7321 tcaatacagc aaaaagaaca ataatcaaaa ctaaaacaaa tccaagaatc acattgcttt
     7381 gctttttcaa ttccttcacc tactttttgt taaaaattcc gctggctaaa taatcaccaa
     7441 tcgttggaaa cagcgtataa aattttgatg caacttctaa aataaacggt tgattgattt
     7501 cccgttttgg atgtctcata ttgcggacga tcatttttgc caacttggca ggttctaaaa
     7561 cgatttgtcc gactttttcc aaatagcttc cgcttggatc agccttatcg aagaaatttg
     7621 tctcgattgg acctggattc actgtcgtaa cagctacgcc aagtggtttc aattctaatc
     7681 gcaaagcgtt ggagaagcca aggactgcaa atttcgtggc agaataaacc gttgattttg
     7741 cagttgccat ttttccagcc atagaggcga tattaatgat atgaccttgt cttctttctg
     7801 ccatatcgat cgcaaatttt tgtgtcaaaa ccatcattcc taagatgttg acttcaaaca
     7861 tatcgtatgc ttttccaagg tcgaatgtaa ggaagtcttc gaaaatccca aaacccgcat
     7921 tgtttaccag aatatcgacg cgcccgactt cttccatcac cttttcatat aattcgtcca
     7981 cgctttcagg attgctgacg tctaccttga aagcataagc ctctttgccg ctcaagcgtc
     8041 tgcactcgtc tctgacacct tctacaaatg ccattcttcg tgcgcaactt ataacgattg
     8101 ctccttgctt tgctgcttca taacagattt gtgcgcccag accgcctgaa ctacctgtta
     8161 ctaaaacaac tttatcttct aaattcatgc ctcttcctcc tcgcgaaatg gaatctcaat
     8221 gctatcaaaa tctttcataa tcgtcgtgtt ttcaaacact tcttgtgctt ctttttcaag
     8281 ctctaaagct gcttttccta aatatctcgc actgatatga gtaagtagca gccgttttgc
     8341 ctgcgcttct tttgcaactt cagcagcttg atgagtcgtg gaatggaaat agttgtgcgc
     8401 cattcttgct tcatctttat taaatgtgct ttcatgtacc agaatatcgc tattttccgc
     8461 aagcacaacg gaattatgtg tttttctcgt atcgcctaaa atagtcacga ttcgtccttt
     8521 tttatctgga cctacgaaat cttttccatt gatcgtccgc ccatcttcta gttggatcgt
     8581 ttcaccttgc ttcagtttgc cgtacagtgg tccagctgga atacctaatt cttttagacg
     8641 atccacctgc aattctcctt tatgatcatg ttcaaccaca cgataaccaa aactgtcgat
     8701 cccatgattt agtttttttg tataaacaga aaattgctga tcagtaaaaa tcggggctgt
     8761 ttcatttagc tcaatgaatt tcagcggata acttaaacgc gtttgcgaaa tacctaaaga
     8821 gacttttatg tattcctcta tcccttttgg cccgtaaatc tctaatggcg tatccccacc
     8881 ttgaaatgac cggctgctga tgagcccggg aagtccaaaa atatgatcgc cgtgtaaatg
     8941 tgtaatgaaa attttaccga tttttctcgg acgaatagtc gttcgcaaaa tctgcatttg
     9001 cgtgccttcg ccacagtcga acaaccaaac ttcattgcgc tcgtctaaaa gtttcaatgc
     9061 tatacttgtc acatttctat gttttgcagg aacacctgca cctgttccta aaaattgtaa
     9121 ttccataatc tacctgactt tctttaagtt cctctttaat tttagggtaa aaagaaggga
     9181 taatcaaccg taagtcgaga atgggttcaa attaaaaata acgttctaaa gcgatagcgt
     9241 tttctttgaa aattactata aaaacagcag cgaacccgtt tgagatcctc tgctgttttt
     9301 tattaatttg aagatccaga actttcattg tcgttggaag tttgcggatg tttgtccttc
     9361 caagtctttt gtgcagcttc tttcgcatga ttttcagctt ctttcgcatc atgcacatct
     9421 ttttcgacat cttcataatc caagcgagta atttctccgc ctaattccat cattaccaat
     9481 tgattcagtg ggcgattgtc ctcttcatca gcaatcaaga tcagtcctgt agctccttca
     9541 ccgattttat ttcctacaaa gtcgaacacg gtctgggcat cttttatttc tttggcatct
     9601 tgtgtcgcac cgatcattcc tccggcaaac catcctaata aaatccctag cggaccacct
     9661 aatacaccaa ccaacattcc gattaatcca cccgtagaag atttgttatt accggtaaag
     9721 tcgataaaat cttctatttt gaattgatgt gtcccattag gatcatggct gacaactgcc
     9781 atctgttctc ctttaatcga acgaacagca tgtaactttt tgatttctga aaatgcctga
     9841 tatgcctggc tttctacttg aaaatttaaa atgattactc gtttgctcat taaaaacgcc
     9901 cctttcatac cttcttgtta tttattttac ctttaattgt aaaataagaa aagaaaagca
     9961 ctcttttttt ccaataaaat agggctgtga caaaagtcgt atcacagctc ctttttccga
    10021 ataaacggcg ttcttgaagc tgatcacttc tgtcacaacc tcccttaaat ctgaatctat
    10081 attttttggt atttgttttt atttgcttgc ctattcgaca aattcaaatt caaactcacc
    10141 gatacgtaca aggtcgccat ctttcgcacc gcgtgcccga agagcttcat ccacacccat
    10201 accacgtaat tgacgagcaa aacgcatcac tgtttcatca tgatcgaaat tcgtcatttg
    10261 gaataatttt tcgatcttct caccagataa gatccacgtt gcatctgaat cacgatcgat
    10321 ttggaactca ggaccttctg gattgaatcc atagtgtaca gtttcttctt ccagttcttc
    10381 ttcgtacaat gggaattctg gcgttacttc taacaaatct gctgtagcat tcaataacgc
    10441 gtccaatcct tgtcgagtca ctccagaaat tggaaatact gggatgtcat cagcaaattc
    10501 atcttctttt tccttgttca gctgttcttt gaatttcact agattttccg gtgcatccgg
    10561 catatccatt ttgtttgcca cgatgatttg cggacgctct agcaagcgta agttataagt
    10621 agataattct ttgttgatgg caagatagtc ttcataagga tctcgtcctt ccatcccact
    10681 catatcgatc acatgtaaaa ttactcgtgt tctttcgata tgacgaagga attgtgtacc
    10741 cagcccaaca ccttgcgaag ctccttcaat caagcctggc aagtctgctg cggcaaaact
    10801 tctgccatca cttgtagtaa ccattcctag attaggtacg agcgtcgtga aatgataagc
    10861 gccgattttt ggacgagcag atgaaataac ggatagtaaa gtcgatttcc cgacagatgg
    10921 aaaacctaca agtccaacgt ctgccaatac tttcaattct agttcgattt ttcgctcttg
    10981 tcctggttca ccgttttcag caatttccgg tgcaggattc cgaggagagg caaaacggat
    11041 attccctcgt ccaccgcgtc caccttttgc cacaactagt gtttgaccat tttcaattaa
    11101 atctcctaaa agagcaccag tctccgcatc tcggacagtg gttccttgtg gtactttaac
    11161 ataggtatct tctgaaccac gaccgtgcat ccctttactc atcccatttt cacctggctg
    11221 cgctttgaaa tggcgattga accgaaaatc catcaatgta cgtaaccctt catcgacgat
    11281 caagatgaca tcgccgccac gaccaccgtc tccgccagcc ggtccgccgt caggaacgta
    11341 tttttcgcga cgaaatgcaa ccattccgtc tccgcccttg ccagctttca catcgatcgt
    11401 tacttgatct aaaaacatgg acataattaa gtcctcctat tattcattta tttatttgac
    11461 tgcctgaatt tccacaataa acaaaagtct tacttatttt atcggtgaaa tctgattttg
    11521 tctatcattt cagcaaaaag attgtttacc ttcttctttt tttatcattt ggtaaggttt
    11581 ccaacctttt ttctgctacc aagtataatt ttttgacaaa atattctttg cgtagcagaa
    11641 taacagtgta ttaattgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11701 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11761 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11821 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    11881 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    11941 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12001 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12061 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12121 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12181 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12241 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12301 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12361 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12421 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12481 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12541 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12601 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12661 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12721 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12781 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12841 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    12901 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    12961 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13021 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13081 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13141 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13201 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13261 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13321 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13381 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13441 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13501 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13561 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13621 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13681 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13741 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13801 atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAFCNS010000072        13819 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFCNS010000072 DAFCNS010000000
VERSION     DAFCNS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.074"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..224)
                     /locus_tag="LNE23_002191"
     CDS             complement(<1..224)
                     /locus_tag="LNE23_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932023.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDFYKVWDSEVFFLAIEDSLLIN
                     LYYSNKNYFKIPAAKTRMKKGVYFLFDIVT"
     gene            complement(751..823)
                     /locus_tag="LNE23_002192"
     tRNA            complement(751..823)
                     /locus_tag="LNE23_002192"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Lys,seq:ctt)
     gene            1204..1773
                     /locus_tag="LNE23_002193"
     CDS             1204..1773
                     /locus_tag="LNE23_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBK6932024.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1760..2620
                     /locus_tag="LNE23_002194"
     CDS             1760..2620
                     /locus_tag="LNE23_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6932025.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2810..3514
                     /locus_tag="LNE23_002195"
     CDS             2810..3514
                     /locus_tag="LNE23_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6932026.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3519..5354
                     /gene="walK"
                     /locus_tag="LNE23_002196"
     CDS             3519..5354
                     /gene="walK"
                     /locus_tag="LNE23_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBK6932027.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5351..6667
                     /locus_tag="LNE23_002197"
     CDS             5351..6667
                     /locus_tag="LNE23_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932028.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6668..7537
                     /locus_tag="LNE23_002198"
     CDS             6668..7537
                     /locus_tag="LNE23_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBK6932029.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7592..8401
                     /locus_tag="LNE23_002199"
     CDS             7592..8401
                     /locus_tag="LNE23_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6932030.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8504..9133)
                     /locus_tag="LNE23_002200"
     CDS             complement(8504..9133)
                     /locus_tag="LNE23_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBK6932031.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9303..10595
                     /locus_tag="LNE23_002201"
     CDS             9303..10595
                     /locus_tag="LNE23_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6932032.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10671..11333)
                     /locus_tag="LNE23_002202"
     CDS             complement(10671..11333)
                     /locus_tag="LNE23_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK6932033.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11558..11842
                     /gene="groES"
                     /locus_tag="LNE23_002203"
     CDS             11558..11842
                     /gene="groES"
                     /locus_tag="LNE23_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBK6932034.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11893..13518
                     /gene="groL"
                     /locus_tag="LNE23_002204"
     CDS             11893..13518
                     /gene="groL"
                     /locus_tag="LNE23_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBK6932035.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4631 a         2367 c         2936 g         3885 t
ORIGIN      
        1 tcagtcacga tatcaaacaa aaagtataca cccttcttca ttctagtttt cgcagcagga
       61 attttgaaat agttcttatt agaatagtag agattgatta ataagctatc ttcgattgct
      121 aaaaagaaaa cttctgaatc ccacacctta taaaaatctt tgacaaattt ttctgatgga
      181 tcgtatttaa accaaagcag atgttcctct agctcgattg tcatgttttt cacctcaacg
      241 aaagtatacg aactaatgtt tgttttgtaa aggataaaaa tagggtagca accggtaccc
      301 aaactagtac ccatttatca aaaacgacga aatttatgta aatagaaaaa taagaataaa
      361 cattgatgta tcaacattta tatatcttta gaacaaatga aaagggtggt tctcataaga
      421 aaaacatgtt cgcacttcta ggcaaaccag gatttgaaga ccttgctaaa gaattgaacg
      481 aacgcctgta aaataaggat ttgtcgctag atttaaaaaa tcagtgatag aaacatgttt
      541 gctttaaaat gaaactagtg atagttttag tgatagtgaa cttaaaaaaa gcactcaatt
      601 tgagtgcttt tttattgtta ttaaaaatta aaaaactaaa atacataccc gaatttcata
      661 cccgaaatat aaaaaatgtc atcacgtaca tctttttcga aactaccgaa gtagccaaag
      721 atgtactgat gacgtgggtt tggcacccaa tgggccgtga ggggctcgaa cccgcgaccc
      781 gctgattaag agtcagctgc tctaccaact gagctaacgg cccgtacaaa aagtacttaa
      841 taatagtagc actaagaatt ttaaaatgca agactacatt taaagaaaat cagaaaaatc
      901 tttacttcct tataggaaat cattcataga ttattcacat ttgctcgcta ttatataagc
      961 ataccaacaa acaaagttct tgcaagagaa caaaccctaa cacttttttc attttttact
     1021 cctttcccgc cgtctccccg gcgggtctcc tgttcttaac gataaaagac aagaaggctt
     1081 agccttcttg ttttttgttt tcatggaatc ccgctaatca aatcacctag ttaaagcaac
     1141 tagattgaat gtttatcata agtctgatat catgtttatg aaatgaaaaa aggagcggtt
     1201 atgatggaaa aaatgcgtca gcaattaatg gagtggggga aggaactaga aacattccgt
     1261 ctgcctcact gggaagaact ccctgactta gaactttata tggaccaagt cagaacgtta
     1321 gttgatcgtt atctttcacc tgtgatacaa ggagaaaagc atccattgct tacctcttct
     1381 atggtaaaca attacgttaa actaggtttg ataccagcac cagttaaaaa aagatataac
     1441 aaagaacacg tagcattttt acttgcaatc actacgttga aacaagtatt gactattcct
     1501 gaaatcaaag aagggatatt gttccaagga aaaacagtag gaattcgtga agcctataat
     1561 ctattttgcg atgaacaaga agcagctgtg tggatggtca gccaattagc acaaggtaaa
     1621 tctcatccgc aaaaattcga ccaggcaacc cctgtagaat atattgctgt aagagcggca
     1681 actttatcgt ttgcaatgaa acttcttgcg gaaaaaacga ttgttctaga aaccgaatat
     1741 ttaaaagagg agaaaacaaa tgaaaaacaa taaaatcgca attttagtag attcaggtac
     1801 ggatgtgcca gaagaattga taaaaaaata cgagatgtat atgatcccat tgaaaattat
     1861 ttataaagat caggtatata cagataaaat tgacattacc tctgaagaga tctatcaacg
     1921 tttgccagag gaaatccctg gtacatcttt gccggatggt gagtcgatca cagaaatctt
     1981 tgagcaaatc aaaaaagacg gttatgaaaa ggtacttgcg gtaacaattt ccagtggatt
     2041 gagcgggaca tataatgtgg ttcgtttaat tgcacaacag caagaagaat tggaagcata
     2101 tgtactagat acgaaaaata ttggtattgg tgcaggtttt tctgcgatcc aagcagcaaa
     2161 atggctggaa gaaggagtag aatgggatca actgatcagt aatctgaatg agttagtcga
     2221 aagaacaaaa gttttcttta atgttgcgac attggaatat cttcaaaaag gtggacggat
     2281 cggtttagtt gcttctattg taggaaccgc tttaaagctt aacccaatca tctcatgtaa
     2341 tgaagaaggg atttattaca cagtaggaaa agctagggga cgtaaaaaaa gtttggataa
     2401 aacggttgct tacgtcaaag aaaaaatcgg taatgcaaag gtcttcaact tagctgttgc
     2461 ccatggggat gcgaaagaag aagcaattga aatggaagcg agacttaaaa gagagttccc
     2521 acaagcaaac caaatctact ttggccagat ttctccggca ttagtagtcc acactggtcc
     2581 gggattattg ggtgtcggtg tacaagtttt agaaggataa atcaaaaaaa ggaaacactg
     2641 aggtgaaaat cagtgttttc cttttttatt gaagttagaa caaaaaataa taaagtattt
     2701 tgcactttca ttttaatttt ctcttttgtt ttataggcaa agatgaaaca aaagcagaaa
     2761 tacggtataa tgagaaggta tgacaataat atataagagg tggctcttta tgaaaaaagt
     2821 tttagttgtc gatgatgaga agccgatttc ggatatcgtc aaattcaatt tagccaaaga
     2881 aggctatgat gtttatactg catatgatgg ggaagaagcc ttggaaaaag ttgctgaggt
     2941 agaacctgat ttgattttac ttgatttgat gctacctaaa atggacggct tagaagttgc
     3001 tcgtgaggta cgcaaaactt atgatatgcc gatcattatg gtaacagcca aagattctga
     3061 aattgataaa gtcctaggct tagagttagg cgccgatgat tacgtgacca agccattttc
     3121 caaccgagaa ttagttgcac gtgtcaaagc aaatcttcgt cgcggtgcca cggctgcgaa
     3181 ggaaccagaa gaggcagcac ctgcggaatt gacgattggt gatttgacga tccatccaga
     3241 agcttacatg gtgacgaaac gaggagaaac aatcgagtta acacatcgtg aattcgaact
     3301 actcttttac ttggcgaagc accttggaca agtgatgaca agagaacatt tattacaaac
     3361 ggtttggggt tatgattatt ttggagatgt acgaacagtg gatgtaactg tacgtcgttt
     3421 gcgtgaaaag atcgaagata atccaagcca tccaaattat ttagtgacac gtcgtggtgt
     3481 cggttactat ttgcgtaacc cagaacagga gtaattggat gaaaagaaaa gtccgctttt
     3541 ttcgatcagt caattttaag atcgctatta cctttattct gattttattg atctcgatcg
     3601 aaattattgg tgcctacttt atacgtggtt tagaacgttc cacgatcaat acatttatta
     3661 aagatatgaa tcaaacggtg gaatcattag caacaacaat cagcccggaa ttgaatcgaa
     3721 aagataatgc agatgatgaa gaggtaaatg cgaatatcaa aagatttatt gaaaacagtg
     3781 cgactagcga tatcatcgaa atacgggtcg ttgacgaaaa agggatcatc cgaggaacaa
     3841 ccgatgtcaa tgaacaaagc gctgtgggaa agaaaaatga ttatgttgat atcaatgatt
     3901 tcacgactaa acgttatgtt gctttagata acgacgataa acgggtgaat atcaatgttc
     3961 aaccgatcct gtccccgacc ggcgatgccg ttatcggggc tctttacgtc aaaagtaata
     4021 tcgaacaaaa atattcggaa atcaataaca cagcagtcat tttctttact gcctcactaa
     4081 ttgcggcatt tatttcaatg atcgtatctg ttttagttgc tcgttcgatt acacagccga
     4141 tcggtgaaat gagagaacaa gccattcgaa tcgctagagg agactacagt cggaaagtca
     4201 aagtgcatgg acaagatgaa cttggacagc tggctgatac cttcaatcag ttagctgaac
     4261 gtattgaaga agctcaagat acaatggaag ctgaacgcaa tcgtttggat agcgtgctat
     4321 cccatatgac agacggcgtc attgcgacag atcgcagggg taaagtcatt acgatcaatg
     4381 atatggctgt ttccttgcta gatatcaaaa acgaggaagc aatcggacaa tcgattttga
     4441 cacttttaga tatcgaagaa gaatatacgt tgcggaaatt attagaagat ccaaacgaaa
     4501 tggtacttga ccgttcaacc agtacattgg aaagtgatca gatgatcttg cgagtagact
     4561 tttctatgat ccgtcgcgaa tcaggtttta tcagcggatt agtagctgtc ttgcatgatg
     4621 tgacagaaca agaaaaaaca gaacgggaac gtcgtgaatt cgtctctaat gtttcccatg
     4681 aacttcgtac accgttgaca agtatgcgta gttatatcga agcattgagc gaaggtgcgt
     4741 ggaaagacga ggaagtagca ccaaattttt tgaaggtaac gctagatgaa acagaccgaa
     4801 tgattcgaat gatcaatgac ctcttaaatc tatctcgcat ggatacaggt aatacccagc
     4861 ttcaattaga atatgtcaat ttcaacgagt tagtgaattt tgttcttgac cggtttgata
     4921 tgatggtggg aaatcaggaa aaaaattata agatccgtcg tgaatttacg caaagagatc
     4981 tttgggtcga agtagataca gataaaatca tccaagtagt cgataatatc atgaataacg
     5041 cgatcaagta ttcaccagat gggggaacga ttacttgtcg cttattagaa acgcataata
     5101 acgtggtttt aagtatcacc gatcaaggat tggggattcc taaaaaagat ctgaaccgag
     5161 tgttcgagcg gttctatcga gtagataaag cacgtgcaag agcacaaggt gggacaggac
     5221 ttggattagc tatttctaga gaagtgatta aggcgcatcg aggcgctatt tgggcagaga
     5281 gcaaggaagg aaaagggtcg actttttata tctcgctgcc atatgaacct tatgaggagg
     5341 aatggtggga atgagaatct ctgaaaaagt cgttcgtata ggactcattt tgatggttgc
     5401 gttaagcata tacttctctt atgcaatctg gctgagccca gcaggaaaaa gttcaatcaa
     5461 cgtagatgag acaaacactc aaatgatcga gtctcaaagc aatcgaaaaa cttccgaagt
     5521 ctttcttccg cttcatgtta catggcacca ttcgggaatg atccaagaaa ccaacagtga
     5581 aaatctcgtg taccgattgc aattgatcat cgataaagca agatttggaa agctttctga
     5641 agtagtttca ggagacaaat cacagtttga acaggtgaaa aatctgaact ctggttttga
     5701 gttggcctat aatgcgcctt ttttgctaag agaatacaag gaagcatttg atttagattt
     5761 agatgtgtcg aacttggcaa aagaaagcga aaatatgtat tttactagaa tacaattcga
     5821 tcaaggaaat aaaaaaatcc gatttttgaa ttattcgaac catctcgtat atgaagcaaa
     5881 tatttcgttg aattggacag atatcaataa agaattaaca gcaaaagaca caaaatggac
     5941 agaaatgaca acagaaccat cgacgatcga cacgcaatat gctacgaaac attcgatcaa
     6001 actgaaaaag tatagttata tcctgtcaca gcaaccctat acacttttta gaaatgcttt
     6061 tttccagaaa ccagatagcg tgaaaaataa tgatgaaagc aatgaattga ttttttacgg
     6121 tgggaatgaa acaatgtcta tccattcgga aacgcaagta gttgattttc gtggagagct
     6181 gccagagaaa aaagaaaatg ctagtgtatt ttctgaaagt ttcccctata ttagcaaatt
     6241 aggcagttct ttagggaacc tcagatattt tgaccgcagt gacaatacga tcgattatcg
     6301 gatattcgtg gaaggcttcc cggtttttgg gacagacagc agaggacaga ttggtgtgga
     6361 agttggcggt gaaacgaatg gttctgtgca agtgaatatc caaaccagtt tgaatacgat
     6421 tcaagtgccg ataccttcag acgaagaagt agaacttgca agtacgaatg aaatcatcca
     6481 gcagttaaaa gctaagggag cagatagcaa caagattggt tcagtgatcg ttggctatac
     6541 atggcaaaat gtcaaagaaa cgaatcgtgt cgttgatctc ttacctgaat ggtatgtgaa
     6601 atataaagat aactggtatt cagctaacga ccttctggaa aaattacctg gattggaggc
     6661 gaattaattg gatttcaaaa aaatcgaatg gatatttttc gtcgcttttt tagggttgaa
     6721 tgtgttttta ttcagcagtt atcatgacgc ggtataccaa gaaaacaatg tcattcgctc
     6781 taatcagact gaatcaatcc aacaacgtct ggctagcgac gatatcactt acacggggaa
     6841 gatttcgtct gagaacaagc aaggctatta tcttagcgcg gagcagacca atcttagcac
     6901 ttcattagcg aattatcgca aggctggtaa aagcggcttg ctggcaagtg gtgtgttttt
     6961 agacggtgat gttttgacga aatctttatc cagttcagat gctgaaaaga acgtgatcga
     7021 tccagataaa attagtagtt ccttggaaga ttttttatct aacaaagaca atattttatt
     7081 cggtaatgat tatgtgtaca cgaaaaactt ttctcattta gaagaagaac cactagaaat
     7141 ccaagcaagc caagagtatg aaggcatccc attcaatgat gatacagcaa aaatcgttct
     7201 ttctttagaa aaaaacgata atgattatag catcacgaaa tacatgcaga cgcatttatc
     7261 tgatatcgag cagctgcgcg aaaagacgga actgtatacg gaagaggaag caatcaatac
     7321 attgtacatc aataacaaaa taccaagagg atcaaaaatt ttatggcgtc agctagctta
     7381 ttcctgtatt ctgaaagtcc gggaaaaaaa tgtgtatgtt cctgtttggc atgtagcgat
     7441 cgaaacaagc gataaagtgg tccaaattga gagtgtcaac gctttcagca atacgatcgt
     7501 gacaaacaat actgtaccaa aagtggaaga tcaataaaaa agcgagtata atgaaaaaag
     7561 tttacaaagt aagaagggaa gtaggagtca aatggctgaa gaaaaaccag cttttaaaat
     7621 cagtatccta gcaagtggaa gttcgggtaa ttcactttat atcgaaagtg aaaaaaaacg
     7681 gcttttggtc gatgcaggac ttagcggtaa aaaaatcact tccttgatgg ctcaaattga
     7741 tcggacaccg gaccaattag atggaatact tgtgacgcat gaacatcgag atcatataca
     7801 tggcgtgggt gtcttggctc gtaagtatca tttggatgtt tttgccaatc agcagacttg
     7861 ggaagcgatg gaaccactga ttggaaatgt accagtggaa caaaaacatc tatttgagat
     7921 ggggaaagtc agaacattcg gcgatatcga tattgagagt ttcggcgtct cccatgacgc
     7981 agcggctccg caattttatc gtttccataa agaaaatcgg tcattcgtca tgttgacgga
     8041 caccggatat tgcagtgatt atatgcgagg tgtcattgaa aatgccgatg gttatttgat
     8101 ggaaagtaat catgatcttg agatgttacg catgggacct tatccgtgga atttaaaaca
     8161 gcgtatttta ggagatagag gacatctttc caacgaagac ggtgcacttg tcatgactga
     8221 tgtgatcggt aatcggacca aacgaatcta tcttggccat cttagcaagg aaaacaatat
     8281 gaaagaactt gctcatttaa caatggaaaa tgttttgaaa gaaaaagatt ttggcgttgg
     8341 acatgatttt gaaatctatg atacagatcc agatgaagca actgagctat tttcgattta
     8401 aacagttcaa tcaataaaaa tataaaaagc agaagataaa atcaatcagg gttggtttta
     8461 tcttctattt ttattttttt agcatattaa aaaaataacc gtattatcga ctgactacac
     8521 tgatgtgctg cttttcatgt ttctcatcca gtatctcatc tactaatgca atcgcgtaat
     8581 ctgccatgct gatcgtactg tcgccagcag cgtttctttg taaataatca tcacttaaca
     8641 aataagtacc tgtacgtttg ccatgtggat caaataaaga agctggactg atataagtcc
     8701 aatgtagatt tttagattgt ttcaatttta acaatccttc gtacatattg aaagctgttg
     8761 gatagaaggg tgcattttta ggtacttggt caatcatacg agtcgtcttt tctgtatcta
     8821 agaataacga agaggcaccc ccaacgacaa ttaagcgtgt atcggttcct tcaagcatct
     8881 caatcaaatg ggacaaagaa gtttgatgca tttcttcttt tcctttggga gcgttgaaag
     8941 catcaacgac aacatcaaaa gatgcaagat cagtagttgt caactcaaat aaatcctttt
     9001 gtataaacgg aacatctgat ggcaattttt ctggatgacg cacgattgcc gtcacgtcta
     9061 aatctctgga caatgcttcg tctaaaataa atgaacctgc atggccagta gctccaatga
     9121 gtgcaatttt cataaaaaac ctcctttttt attcctgtcc attgtaagca gttgctgaat
     9181 attcgtcaaa gaatccgatt tttgaaaagc tcagtgcgca ttcctttcat ggttcgtgat
     9241 cattgggtat agttaaatta agaaaactga ttgataaaaa tagaagttga aggagataag
     9301 aaatgaaaga gtggttagat cgtgcctttt taccaatgat aacatctgtt gtgaatctta
     9361 gacccttaac tgctttgcgt aatggaatga cttacacgat cccaataatt attgttggct
     9421 cgctgttttt cttatttgct cattttcctg tggattacat cgctcagtgg atagacaaaa
     9481 caggattttc tatgtatttt acacaagtat accaggctac atttgatgtg atagcctttt
     9541 ggatcgtttt tgccattgca tacgcttatg caaaagaaga agacatcgat cgcttaccag
     9601 tcggcataac agccttgtct gcttatctac tgcttattca gccactaaat actcagatag
     9661 atactgtctg gacaggtgct gaagggatac taggagcagt tacaactggg ctcatcatcg
     9721 gatggggcta tgcacgttta gtaagacttg cgaaagggtg gaagcaaaga gagaagatac
     9781 ctgaaaatgt ttggcagtct tttacttcat tgattccaat cattgttata gtcactttta
     9841 cgcttatcgg ttcagcggtt tttcaacagt tgttcagaat gactgttgtt caaggagtat
     9901 ggacatatgt ccagcttccc attcaatcgt tcatagacac gttgtttggt gtgcttttat
     9961 taggtttttt tgttccgttt ttatggttat ttggtcttca tggatctgcg atggtcaatg
    10021 ggttggttag tcccgtcttg caagcgaatt cattagcgaa tgcagaaatc ttagcctcag
    10081 gacaagagtt gacggtggca aatggcggac atatcgttac tcagcaattt ctcgatcagt
    10141 tcatgacggt tacgggtgct ggattgacgt taggggctgt attttttatg acggtttttg
    10201 cgaaatcgaa gaaatacaga gaattaggaa atcttgctct tttaccagcc tttttcaaca
    10261 tcaatgaatc aattattttt tccacaccga tcgtgatgaa cccgatgatg gctgttcctt
    10321 ttattttcgc accggtcgtt tcaggattga ttacctattc cgctttatat tttggattcg
    10381 ttcctttatt tacagcagtc caagtccctt ggacaacacc gccgatcctc tcgggtttct
    10441 tagtaggcgg agtgcctgca gcgattttac aaggtatcgt gttgtcaatt agttttttca
    10501 tttatttccc atttttgaaa aaaatagatt ctgcaaactg caaaaaggaa agacagacaa
    10561 aaaatgatgg agcagcagaa aagatgaacg actaaaaaac aaaaatactc ctgtcgcttt
    10621 agattgggaa aatcaccaat ctaggatgac aggagtattt atagtcatgt ttatactagc
    10681 cctttttgga cagctaattg aacaagaaca actaatgtgt tcattcctac gtgtgtgatc
    10741 atcgatgtcc agatccgacc agttgctttg tactctaggc tgaagaagaa acctaggaaa
    10801 aagtagatta gcagatgacc atcattatga gcaaacgcaa acagcaaaga gctgatcact
    10861 gctccgaccc aaaaatttga atatcgactg atgatcccca gtatactgcg gcggaatacg
    10921 aactcttcca tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg tgcttccatg
    10981 atcatctgga tgatattttg tgtattctcg gaaggggttg ctttgccaaa caatacagat
    11041 tctaacatga cagcgatgct ttgcagaatc aatgcgacaa agattccaac caatccataa
    11101 ataacaatct ttggagcaga agcttttttt accgctgatt cgatcgctaa gggttcacgt
    11161 tgcttaaaat aaaaaatagc taggacaacg gcgcctagta tatacatgac tgcagttgct
    11221 gtgatcaatt cagacgtaga ttttacaagt cctatcgaag ctaggatcaa gggtgagata
    11281 aaaattagac catagcaaaa aatgctgagg aagctatatt tttttaaaga catgtaattc
    11341 ctccttttaa gggataacaa gcatgcaagg caagcttata tcgattgatt tcaattatat
    11401 cataatttca ataagcagct gtcagaataa aatttctttt ctttgattta aaagcttgca
    11461 aaaaagaggg agaatgagta ttattaaaaa tgtaggttag cactcgaagg ttaagagtgc
    11521 taacagatta aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat taggtgatcg
    11581 cgtcattatc gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg ttttagcttc
    11641 tgctgctaaa gagaaacccc aaactgggac agttgttgca gtcggtgaag gccgtttgtt
    11701 agaaaacggt gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta tgttcgaaaa
    11761 atacgccggt acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg caggaaaaga
    11821 tatcatggcg attgttgagt aaatttggaa gataaaaaaa taatcaaaaa taatcaatga
    11881 ggtgaatcaa tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc agctatgtta
    11941 cgcggggtag ataaattagc agatacagtg aaagtaacat taggacctaa aggccgaaat
    12001 gtcgtactag aaaaatctta cggctctcca ttgatcacaa atgatggtgt gacgatcgca
    12061 aaagaaatcg aattagaaga tcattttgaa aacatgggtg caaaacttgt atcagaagta
    12121 gcatcaaaaa caaatgatat tgcaggagac ggtacaacga cagcaactgt tctgacacaa
    12181 gcaatcgttc gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct aggcattcgt
    12241 cgcgggatcg aattggcaac aaaagcagca gtcgaagaat tgcataatat ctcaactgtt
    12301 gttgattcaa aagaagccat tgcacaagta gcagctgttt cttcgggttc tgataaagtt
    12361 ggacatttga ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat cacaatcgaa
    12421 gaatcaaaag ggatcgaaac agaactagat gttgtagaag gtatgcagtt tgatcgtggt
    12481 tacttatcac aatatatggt aacagataac gacaaaatgg aagctgttct agaaaaccca
    12541 tatatcttga tcacagacaa aaaaatctct aacatccaag atattctgcc attgttagaa
    12601 caaatcttgc aacaatcacg tccattgttg attattgctg acgatgtaga tggtgaagca
    12661 ttgccaactc ttgtattgaa caaaatccgt ggaacattca acgtagtagc tgtaaaagct
    12721 cctggtttcg gcgatcgtcg taaagcaatg cttgaagata tcgctatttt gacaggtggt
    12781 acagtaatta ctgacgatct aggtttagaa ttgaaagatg taacaatcga aaacttagga
    12841 aatgcttcta aagttgttgt agataaagac aatacaacga tcgtagaagg ttctggagaa
    12901 aaagaagcaa tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga aacaacttct
    12961 gattttgacc gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg tgtagctgtt
    13021 gttaaagttg gtgcagctac tgaaacagaa ttgaaagaat taaaattacg cattgaagat
    13081 gcattgaatg ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg tggtacagct
    13141 ctagtgaacg ttatcagcaa agtttctgct gtagaagcag aaggagacgt ggcaacaggt
    13201 atcaagatcg ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga aaacgctggt
    13261 tatgaaggat cagtgattgt tgataaattg aagaacgtgg aactaggcac tggtttcaat
    13321 gctgctactg gagaatgggt aaacatggtt gaagcaggta tcgtagaccc aactaaagta
    13381 acacgttctg ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac gacagaagca
    13441 gtcgttgcag ataaaccaga accagcagcg ccagcagctc ctgcaatgga tccatcaatg
    13501 atgggcggta tgatgtaaga aaaagcaact tgcttatcta caactgatca ttagtgaaag
    13561 acagataaaa agaggctgga acagaagcgt ttaactccaa gaaataagaa gaaattcacg
    13621 gaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt
    13681 ccgctgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtcccacgct
    13741 cttttttgtg tgtaattata atgaaaatag ttatttttaa tagttataat attttatcta
    13801 ttaaaagtaa atatccatt
//
LOCUS       DAFCNS010000073        13780 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFCNS010000073 DAFCNS010000000
VERSION     DAFCNS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.075"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(63..1268)
                     /locus_tag="LNE23_002205"
     CDS             complement(63..1268)
                     /locus_tag="LNE23_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6932036.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="LNE23_002206"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="LNE23_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="LNE23_002207"
     CDS             complement(1447..2832)
                     /locus_tag="LNE23_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK6932037.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="LNE23_002208"
     CDS             complement(2861..3247)
                     /locus_tag="LNE23_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6932038.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="LNE23_002209"
     CDS             complement(3263..3577)
                     /locus_tag="LNE23_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6932039.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="LNE23_002210"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="LNE23_002210"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBK6932040.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="LNE23_002211"
     CDS             4837..6126
                     /locus_tag="LNE23_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBK6932041.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="LNE23_002212"
     CDS             complement(6165..6770)
                     /locus_tag="LNE23_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBK6932042.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="LNE23_002213"
     CDS             complement(6966..7430)
                     /locus_tag="LNE23_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6932043.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="LNE23_002214"
     CDS             complement(7452..7772)
                     /locus_tag="LNE23_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK6932044.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="LNE23_002215"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="LNE23_002215"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBK6932045.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="LNE23_002216"
     CDS             9194..9544
                     /locus_tag="LNE23_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK6932046.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="LNE23_002217"
     CDS             9729..10682
                     /locus_tag="LNE23_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6932047.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..12482
                     /locus_tag="LNE23_002218"
     CDS             10695..12482
                     /locus_tag="LNE23_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932048.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            12469..13671
                     /locus_tag="LNE23_002219"
     CDS             12469..13671
                     /locus_tag="LNE23_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932049.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         3950 a         2694 c         2529 g         4607 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
    11101 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
    11161 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
    11221 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
    11281 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
    11341 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
    11401 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
    11461 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
    11521 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
    11581 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
    11641 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
    11701 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
    11761 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
    11821 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
    11881 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
    11941 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
    12001 ttctatcact atgggcaaga aatttaccaa gtaatataaa aatctataca gatgcttata
    12061 ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa tagttacggc tttttagata
    12121 ctcgtgtaaa cacgaataat tatggtatga agcaagtaga tttgattgaa catcttactg
    12181 gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt ctttggttcc ggcacattac
    12241 tgtggttagc tatcggcagt gcacttttag caatctttaa gaaaaaatat cggtatttac
    12301 tcctttttct tcccggtatg cttgtttttg ctactattat gattgctact cctgtggctt
    12361 ttagtttacg gtatgtgttt gttttagcat taggtctacc tgtttatatc ctctctcctt
    12421 ttttactaga taaaagagaa aaaatgaaaa cagatatagg agatactcat gaaactattt
    12481 aaaaagatat tttttgcttt tgttctcttg atcgctagct attgcgtact actgacaatt
    12541 acttttttca ttccgcagca ctctattgaa tcgaatgcag aaaaatctct acagatggtt
    12601 gaaaaagaag gaatgtatcc ttctatcaat caaggaaata tgctaggcac tcggttagat
    12661 aattttactg atcatttgat gatacgaaaa acaaaagcag atcctgaatt gaactccttg
    12721 gagaacgcga tgtctatggc ggattatccc cgttattggc atggttatca gcttttttta
    12781 cgacctttac tgctagggat gagtttgggg agtattcgaa tgatttacgc agccattctt
    12841 tttctgctga ttggattgac gagctacttt ttaaccaagt gtagcgacat ctatttaacg
    12901 acagccttgt tgatttcgct tgctatagga aatgctgcaa cctttttctt cagtatgcaa
    12961 ttttcaaata tttggatttt gactttattg tctgtattac tatttttaac aaaaccacaa
    13021 ctctccaaca ccaaccaaca attattcttg tatttctttg tcattggttc tttagctaac
    13081 ttttttgact tgttgacaac accggtcatc tcttggggga tacctgtgat cattatttat
    13141 tatgtaacaa ataagtattt aatggataaa cgcagttcat tgagcaaaca agtaggcagt
    13201 tttgttttta ccggtatctt ttggggatta ggctatggtt tgacatgggt ggctaaatgg
    13261 atcctatcat cgatcatatt gaacaaaaac attgtaaaag atgcaatcaa ccaaatcctc
    13321 tttcgaacgg aaggaaacga caaatacccg ttgcatcgat tagagatgct gaaaagcaat
    13381 attagattga tgtatccaaa agtagcaata ttattgctat tgattacttg tcttgttttt
    13441 ctctattttg cttatcgtaa acgaaatagc tggcgtccgt tccaacttct agttttgttt
    13501 gttttagctg ctgtaacgcc gtatatttgg tacaatattt tagctaatca ctcacagatt
    13561 cattattggt ttacgtatcg tacgcaaatc atcacaagtt ttgctatact tagtgctttc
    13621 gcctttttga tccctcctga gaaatcaaaa gacgaactaa attactttta agaataccga
    13681 tgtactaatt catttttaaa cataaaaact ccagctgtca gagatagccg gagttttttg
    13741 gctctataat ttatgggact gatgaatcag aattgattct
//
LOCUS       DAFCNS010000074        13302 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFCNS010000074 DAFCNS010000000
VERSION     DAFCNS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.076"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            60..2849
                     /locus_tag="LNE23_002220"
     CDS             60..2849
                     /locus_tag="LNE23_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK6932050.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            2896..3423
                     /locus_tag="LNE23_002221"
     CDS             2896..3423
                     /locus_tag="LNE23_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBK6932051.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3444..4634
                     /locus_tag="LNE23_002222"
     CDS             3444..4634
                     /locus_tag="LNE23_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6932052.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4674..5972
                     /gene="asnS"
                     /locus_tag="LNE23_002223"
     CDS             4674..5972
                     /gene="asnS"
                     /locus_tag="LNE23_002223"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBK6932053.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7148..7822
                     /locus_tag="LNE23_002224"
     CDS             7148..7822
                     /locus_tag="LNE23_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK6932054.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            7819..8160
                     /locus_tag="LNE23_002225"
     CDS             7819..8160
                     /locus_tag="LNE23_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932055.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            8190..8549
                     /locus_tag="LNE23_002226"
     CDS             8190..8549
                     /locus_tag="LNE23_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBK6932056.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9074..11158
                     /gene="cadA"
                     /locus_tag="LNE23_002227"
     CDS             9074..11158
                     /gene="cadA"
                     /locus_tag="LNE23_002227"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK6932057.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            11449..12915
                     /locus_tag="LNE23_002228"
     CDS             11449..12915
                     /locus_tag="LNE23_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6932058.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4403 a         2311 c         2876 g         3712 t
ORIGIN      
        1 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
       61 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
      121 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
      181 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
      241 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
      301 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
      361 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
      421 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
      481 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acaggtcaac
      541 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
      601 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
      661 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
      721 tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa
      781 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
      841 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
      901 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
      961 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     1021 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     1081 tcaaaagcta tcgccactat attgatttgc agcgttttaa aggcacactt gtggaaccgc
     1141 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     1201 agactggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     1261 tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     1321 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     1381 tagtacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     1441 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     1501 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     1561 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     1621 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     1681 gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc
     1741 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     1801 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     1861 agatgcggat cgatttagca agtttatata gcctatttga tcaattagac aagcaatact
     1921 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     1981 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     2041 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     2101 gccctaatcg acactatctc tctaagaggc ttggcattga agcagcacct cttaaagtga
     2161 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     2221 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     2281 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     2341 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     2401 ggtttggtgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     2461 tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggagatgct ttgcagatcg
     2521 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     2581 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     2641 tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc
     2701 ttattttgga tcgaagacta gtcactgcaa aatatggcgc acggatcatc catgcattac
     2761 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggat atccatgaat
     2821 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     2881 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     2941 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     3001 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     3061 gtagaaatcg caaagaaata tgcaaatctt gaggaaaccg atcattttta ttggtttact
     3121 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     3181 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     3241 aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     3301 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     3361 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     3421 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     3481 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc
     3541 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     3601 tcgaagcaat ccgcaatggg aaagctagct attatacacc aacagcagga atcccagagc
     3661 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     3721 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     3781 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     3841 aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta
     3901 ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     3961 catcgaatcc gactggaatg atttatagca agaatgaact gcaggcaatc ggtgaatggg
     4021 cagtaaaaaa tgatattttg atagttgctg atgatattta tggccgttta gtttataatg
     4081 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag accatcatca
     4141 tcaacggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggaa
     4201 atcctgtgat catcaatgga atgatcgctg ttgcttccca atcaactagc aatccgacag
     4261 cagtcagtca atatgcagct gttgaagcat tgaaaggtga gcaagataca gtagaagaaa
     4321 tgcggaacgc ctttgaagaa cgcctgaata cgttatatcc gcttgtggca gaactgccgg
     4381 gtgtttctct tgaaaagccg caaggagctt tctatctttt tcctaatgta aaagaaacgc
     4441 tacgcatgtg taagtatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     4501 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     4561 ccactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     4621 aaagtcaaaa ttaatcctga agaaattcaa ggaatataaa aggagagtca tacgtggaac
     4681 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     4741 tcgcaaataa gcgatcaagc ggaaaaatcg catttttaca attaagagac ggaacagcct
     4801 ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa
     4861 atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa
     4921 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     4981 cgatcacacc aaaagaacac ggaacagatt tcttgatgga tcatcgtcat ttatggctgc
     5041 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     5101 aattctttaa tcaacatcat tttgtcaaaa tcgatccgcc gatcttgact ggttctgcac
     5161 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     5221 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     5281 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga
     5341 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     5401 tagcattctt agtacaaaat gttttagatc attgcgaata tgcattagat gtactaggac
     5461 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttacgatg
     5521 atgcaatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     5581 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     5641 atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtaa
     5701 tttgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     5761 caacagacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     5821 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     5881 gccttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat
     5941 tcccacgttt gttgaaccga atttatccat aagaggtgat gaaaaagctg ttctgcatca
     6001 agtaattaga acagccaagc aaaaacagct gttcatgttt ttgaattggc gggacttatt
     6061 accgcagatg taagcttttg aataccgttt agtggagagg ttgtgaaaaa gctgtttagt
     6121 ttcgagtatt aagaaaaatt ttgtggaaat ggctttccac atttttgcat aaatttggct
     6181 taataccgag aaactggctt ttgaacaccg tttaaggaag aggttgtgaa atcagtgcct
     6241 tgacctgagt attagagaaa caaaacggaa aaatagcttc tcatatttta ccgttttttt
     6301 agctaatatc gagggtcagc tgattgaaca ccgtttatat caagaggttg tgcgcctgcc
     6361 gttcagctcc gagtcacaag gagtaatttc aaaaaacagt ttttcatgtt tgatgactat
     6421 tgtgacttgt gaccgaggag ttggccggag agcaacgttt atttaaaaaa aagaactgcg
     6481 aactctcgca gttctttttt taaaatacgg gataaaggaa gtttccagca gacaagagaa
     6541 gaaagcaaaa gaaattgcta tttgattcac aaggttctat aacgaaatcg caatcaatca
     6601 aacatataca aagaaggtta ttcaaatgga aatctgattt gatgatgctt ctgctgttga
     6661 aataaagcag catctagtac ctgaaaacaa actaatagta acattcgaag atggagtagg
     6721 tccgtatttt cagcatgcaa tgatccatat gtaagtttaa ttcatccttg aaatcatcgg
     6781 accagataaa gttatgacca atctatccca agcaatctag aagagatatt agtaaaagaa
     6841 catttactgg aagtcttaga tgagcctata aatatatgtt ctgatggcgt catcagtggt
     6901 ctgattgacg gaagcttagg tgtgatcgat ttctctggac caaacaacga aggaatcaag
     6961 gggaatccgg cacgttaaga aaaaaagtat aaaaaactag acacttgatg agcgttcatt
     7021 caattgattg aagggactag gctggtgtca ttttttatac atatattttc tctttcgtct
     7081 tgatagggct tatgggataa tataaggaag aaaatcatcg ttttattcaa aaaggagttt
     7141 ttgacaaatg aaaaaacgta tattatttat cggtgacagc attacagacg caggaagaga
     7201 ccgagcagaa gcaactgatc taggtaaagg ttatccactt ttgattgccg gaaaactaat
     7261 ctcccaatat ccaacgatga aatttgaatg catgaaccgt gggataggag gagacaaaat
     7321 tgcagatctg caagtacgct ggcaagaaga ttgtttagct ttacaacctg actatgtatc
     7381 gattttgatc ggaatcaatg atacctggca taatactagc gatcaggcta tattcggaac
     7441 tgagaaatct gcgcagcaat ttgaatccgt ttatcgaaaa ttactaaatg aacttcaaac
     7501 agcggaaatc tgcaacatca tcttgatgga gccgtttgta cttcctataa ccgacgaccg
     7561 tcatggctgg cgtaaagact tagaccctaa aatccaaatt gttcgtaaac ttgctaagga
     7621 atatggcgca ttgctggttc ctttagatgg catattcaat gctttaggga ttgcttatgg
     7681 tttttcgaaa tatacaagag aagatggggt ccacccaacg attggcggac atgagatcat
     7741 tgcacaaagc tggatagatg caattgccgc agtcgatttt tttagtggag ataaaaatga
     7801 gcgaaagaaa agtgagaaat gatggaaata cggtgggcag taaaagaaga tttacctcaa
     7861 atcaatcagc tgattactga tcatccagaa tcattcgtaa aagcaaaaac ggcagctgac
     7921 ctaaatgata agaataaact attgctcgtt gctgtaacaa aagaaaaacg gattatcgga
     7981 tttgcgcaag cggacgtgca acagtcagaa gaacgggtgg atcagcgacc atcaggaaaa
     8041 ctaatccaac acacattagt aacaaaagaa aatacggaag aaatccaaaa aaaatttgca
     8101 gaatacttga tgctggaagc taaaaaaaga agaataaaat caatttttgc ccagttttaa
     8161 tcaaaaacag taatagaagg agaaatacaa tgagttgttt aggaaatgtc atctggttta
     8221 tattcggcgg actaatcggt ggtcttagct ggttagcagc ggggatactt tggtgtatca
     8281 cgatcatagg tatcccaatc ggtatgcaat gttttaagat cgcctccctt tcttttgcgc
     8341 cttttggcaa agaagtaatt tatcaccagt ctggcatgtc attgatcgtt aatatcatct
     8401 ggctgatcgt ttcaggccta ccaattgctc tcgcacatgt cgtcagcggc atccttctat
     8461 gtatcacgat catagggata ccttttgcca agcaatcttt caaacttgct cgattggcgt
     8521 tgatgccatt cggtgcagaa attgtataaa aagagaataa taaaggttag aaaacgcata
     8581 gagtcaggtg ggatacatgt atttcacctg actctatgtg ttttcctgta taagttctcc
     8641 catgtgactt ttacgtcttt tatttatatg ggattactgt ttatggaaag ttgaaataat
     8701 tttcattaaa gaactctttc tatatttttg aaagcgtagt ggagcttcga atatctggga
     8761 aggacatttt gcagggattg aaaaaagata ttgtcctatc gaaaaattaa tgaatttaga
     8821 caaattgcca gcaacaggct tcaaacttag ttgctatcct gtcaagatca agaaggcaag
     8881 tgcgggttgg ataagagcag gggcaatgat cgaagagaaa aagaaagaat aagccaaaac
     8941 atctgtatgg aagaaatttt ccgatgcaga tgtttttcat tttttctttc ggataccttg
     9001 acatatgaat aaccattcat ttataattag ttatgaagag atgttcatat gttgaggcga
     9061 ggaggtcatt aaaatgaaaa gttacagatt agaaggttta gattgtgcga actgtgcaat
     9121 gaaaatcgaa aaaggagttc agaagatcaa tggagtcaag gaagcaacgg taaacttcac
     9181 ttctggaaaa ctaacgattg acgcagagga agatcatttg gctacgattg aacaagaaac
     9241 aaaaaaagta gtgaaggaat tagaaccaga tgtcaaagta acagaaatag ataaggaaaa
     9301 agtatctgaa catggaaatg aaaaagagag aaatacgctg ttccggattt tattttccct
     9361 tgcaggtatc gctttattgc tattatttga ttttaatgaa ccaattcgct tgatagggta
     9421 tttattgatt tatctgctga taggatatga tgtagtgaag aaggcagtga tgaatatcgt
     9481 caaaggaaaa atctttgatg agaattttct tatgtctgtt gctacgattg gcgctatgtt
     9541 gatcggtgaa tatccagaag ccgtagcagt gatgctattt tatcaaatcg gcgagtattt
     9601 ccaaggattg gcagtcagtc attcaagaaa atcgattcgc gaattgatgg cgatccgtcc
     9661 agaaacagct cacgtgcaga cgacggaagg attgatgacc gtgaaccctg aagacgtgca
     9721 gatcggtcag tttgtacttg tgaagcctgg tgaacgtgta ccgttagatg gaacgatcat
     9781 cgaaggagaa tcattagtcg atacgtcagc acttacagga gaatcagtcc ctaaatctgt
     9841 atatgttggt gaaacagtat taagcggatt tatcaataaa aataaaccat ttttagttca
     9901 agtagaaaaa tcttatgaaa attctactat cagcaaatta ttagaattag tagaaaatgc
     9961 cagcagtaaa aaagcaccag ctgaaaattt cattactaaa ttcgcgcgct attacacacc
    10021 agtcgttgta ggattggccg ttttattagc tgttttacct ccacttgttg tttctggagc
    10081 ggcgttttct gaatggatct atcgagcatt gacattttta gtgatctcat gtccatgtgc
    10141 attagtgatt tccgtgccac ttagtttctt tggcggtatc ggaggcgcaa gtaaaatcgg
    10201 tgtattagta aaaggttcaa attacttgga gttgcttgcg caaaccgaga cagtggtatt
    10261 cgataaaacc ggcacattaa cgaaaggtga cttttcgatc cagacgatcg atacaaaaat
    10321 cgatccaaag aaattcatgc aatatattgc gagtgcagaa cagttttcta cacatccgat
    10381 tgctcaatct gttttagaag gctacgcagg tccgctttta ccaacaacta atatccagga
    10441 atttgcaggt gaagggatct tagctgaagt tgatggcaaa caagtattag caggtaatca
    10501 caaattgatg gaaagatttg aaatctcttt tcctttaagt caagaaatcg gtacattgct
    10561 ttatctagcg atcgatcaaa gttacagtgg ttatcttgtt attgcagata ccttgaaaga
    10621 agatgccgta gacgcattgg ttcaattaaa acaggctggt gtgaagaaca cagtcatgtt
    10681 gacgggcgac tcgaaaaaaa ttgctgatca tatcgggaag caagtaggcg tggacaagat
    10741 atatagcgaa ttgcttcctg aagataaagt gcagcggtta gaggaaatct tacaaagaaa
    10801 caataaaaag accgcatttg tcggagatgg aatcaacgat gcaccggtgc tagcaagagc
    10861 agatgtagga atcgcaatgg gtggattggg aagcgatgct gcaattgaag cagcagatgt
    10921 agtcatcatg aatgatcaac catcgaagat tgcagaagca atccatttag caaagaaaac
    10981 attgaaaatc gtcaaacaaa acattgtctt tgctattggg ataaaaattt tggtacttgc
    11041 cttaggtgct tttggttttg catcaatggg agatgcagta tttgcagatg ttggggtgac
    11101 agtattagct gttttgaatg ccatgcgaag cttgcatgta aaaaaagagg acgaatgaac
    11161 gtcctctttt ttttatatcc ttttcttaat gaaaaatgta aagaaaatag ataggattat
    11221 aaaataacta atgtttttaa ttttgcagaa aattcaaaat agcttgaaat tcaataaggt
    11281 caatgaaata aaagaaaata acttattttt ttgatggaaa aaatatgaaa aaacgatgtg
    11341 tattaaataa aagaatacac acataaaata acactgtctt gtaagtttgt taaaaattga
    11401 atatgattac atctggataa caaattttta tcataaagga gagaacgaat gaacaacaaa
    11461 caattacgct ggtttactgt tgcgttaatt gcatttaaca tggtttgggg catggggaat
    11521 gtagttaata actatgctca gcaaggagta tcagttgtca cctcttggct attgattttg
    11581 gccatttatt ttattccata cgcattgatc gttggacaat taggatcagc cttcaaggat
    11641 agtaaaggag gcgtcagttc atgggtcgaa aatacgacat ctaataaacg actggcatac
    11701 tatgccgctt ggacttactg ggtcgtccat attccttatt tggcacaaaa gcctcaagca
    11761 attctgattg ctggtggttg ggcagtccaa ggaaatggaa atatcgtaaa tacgatgtca
    11821 gtacaaatgg tagcagttat ttcacttatt atatttcttg cctttttata tttatcaaca
    11881 aaaggacttt ctactttgaa agtgattggc ggactggccg gaacagcgat gttcgtcatg
    11941 tcgatcttat ttattctttt agctgtaacg gcaccgttca tcaatccaac gatggaattt
    12001 gccacaccac atatgaatca agtgaaaaca tatattccaa catttgattt cagttatttc
    12061 acaaccgttt cgatgctagt atttgcagtt ggaggggcgg aaaaaatatc cccttatgtc
    12121 aactctacaa gaaatcctgc aaaggaattt cctaaaggga tgatcttctt agcagcaatg
    12181 gtagggattt gtgctgtatt aggctcagtc gcaatgggta tgctgtttgc tagtgataat
    12241 attcctaaag acttgatggc aaatggggca tacagtgctt ttcaaatttt aggtaattac
    12301 tatggtatcg gtaactcatt gatgatcatt tacgcattga caaacatgat tggacaaatc
    12361 tccgcattag ccttttcaat tgatgcacca ctaaaaatct tgttagcaga tgcggatcca
    12421 gaatttgtac caaactggct acgtaaacgt aatcgtaaag gtacattggt gaatggttat
    12481 cttctgacag gtatactagt aggaatcatt attttgttgc cgatttttgg tatcaaagat
    12541 atgaatgaat tagtaaaatg gttgacgaac ttgaactcag ttgtcatgcc aatgcgttac
    12601 ctatgggtgt tcttggcata tatgatgttg aataaagctt ataagaaatt caattctgaa
    12661 tataaatttg ttaaaaatcc aaaaatcggt tttatctttg gtgcttggtg ctttgccttc
    12721 actgcgtttg catgtatcct aggaatggtg ccaaaagtag attatactgc tgatcctaaa
    12781 gcatggtggt tccaattatt tacgaatatc atcacaccaa tcgtcttgat cttattaggt
    12841 atgatcttgc cagcaattgc tcgtcgagac aaaaacaaag aaatcactga accagaaaca
    12901 gtggttcagc cttaaaatag aaatacttgt tacaagaggt tgtgacagaa gtggtcagct
    12961 tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa gtttcggctt
    13021 gtttttcgaa gaagctactt ctggaacacc gtgaatcaga ggttgtgaaa aagctgtcct
    13081 gcatcaagta ataagaacag ccaaacaaaa atagcccctc atatttttcg tttggctggg
    13141 cttattaccg cagatgcaag ctttggaaca ccgtttaagg aagaggttgt aaaatcagcg
    13201 atttgacctg agtattagag aaacaaacgg aaaaatagct cctcatattt tgccgctttt
    13261 tgagctaatg ctgaaggtca gctgattgaa caccatttat tc
//
LOCUS       DAFCNS010000075        12993 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFCNS010000075 DAFCNS010000000
VERSION     DAFCNS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.077"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..760
                     /locus_tag="LNE23_002229"
     CDS             59..760
                     /locus_tag="LNE23_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBK6932059.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            757..1878
                     /locus_tag="LNE23_002230"
     CDS             757..1878
                     /locus_tag="LNE23_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBK6932060.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1893..3125
                     /gene="pepT"
                     /locus_tag="LNE23_002231"
     CDS             1893..3125
                     /gene="pepT"
                     /locus_tag="LNE23_002231"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBK6932061.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3257..4165
                     /locus_tag="LNE23_002232"
     CDS             3257..4165
                     /locus_tag="LNE23_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBK6932062.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4200..4472
                     /locus_tag="LNE23_002233"
     CDS             4200..4472
                     /locus_tag="LNE23_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBK6932063.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4483..5529
                     /locus_tag="LNE23_002234"
     CDS             4483..5529
                     /locus_tag="LNE23_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBK6932064.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5780..8389
                     /gene="clpB"
                     /locus_tag="LNE23_002235"
     CDS             5780..8389
                     /gene="clpB"
                     /locus_tag="LNE23_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBK6932065.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8540..9226)
                     /locus_tag="LNE23_002236"
     CDS             complement(8540..9226)
                     /locus_tag="LNE23_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932066.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9204..9698)
                     /locus_tag="LNE23_002237"
     CDS             complement(9204..9698)
                     /locus_tag="LNE23_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK6932067.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9718..10938)
                     /locus_tag="LNE23_002238"
     CDS             complement(9718..10938)
                     /locus_tag="LNE23_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6932068.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11085..12920
                     /gene="lepA"
                     /locus_tag="LNE23_002239"
     CDS             11085..12920
                     /gene="lepA"
                     /locus_tag="LNE23_002239"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBK6932069.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         4256 a         2378 c         2727 g         3632 t
ORIGIN      
        1 ttcctagtct ccttatttta tttggaaaat gctataatta tctagaagga gtgaagctat
       61 gaaccattta gatttatctc agcgcttagc cgcagtgggt gactttgtcc ctgaatccgc
      121 tcgtttagca gacattggat cagatcacgc ttacttaccc gttgcattga tattaagagg
      181 aaaaatcgaa tatgcagttg ctggtgaagt cgttcaaggt ccttatgaat cagcacgtaa
      241 gcaagtcaaa aaagatggat tagaagaccg tatcaccgta cgccttgcaa atggtctaga
      301 tgctatccag ccacaagatg gtattacagc tgtcacgatt gcgggtatgg gaggttcact
      361 gatccgagat atcctagaag ctggcaagtt gaatcaacgc ttaagcggac aagaacgttt
      421 gatccttcag ccaaacatcg gagagcgtac attacgatct tggctgcaag aaaatcagta
      481 taaaatccaa tcagaaaaaa tacttgaaga aaataaaaaa atatatgaaa tcattgtagc
      541 cgaaaaagaa caaaaaccag ttacttacaa tgaaatggag ctgatgttcg ggccatttct
      601 gcttgaagag aaaagtgaga tcttcaaatc aaaatggaca agagagcttc aacaaagaaa
      661 agctatccgt gaacagctca aacaagcagc taatcaagag gcacgtcaaa aacaaatcga
      721 gcaggaaatc caaatgattg aggaggtttt aaagccatga caatcagcgg acgcactttc
      781 atccaaaagt ttgaaagcta ttgtcctcag tggctagctg aagaaggaga cccagtcgga
      841 ctgcatatcg gaacattgga taagccaatc caacgagtaa tgatgacatt agacgttcgt
      901 ccagaagtgg tcgaagaagc aatcaagaaa aaaatcgatt tgctgattgc taaacatcct
      961 ccaatctttc gtcctgtaaa acggctggtt actgatcagc cacaagaaaa aatgtatgca
     1021 gatttgttga aacatgatat tgccgtctat gcagcacaca cgaacatgga tattatttgg
     1081 gatggactga atgactggtt ttgtgaactt ctcggtattg aagtggagag ttatttagta
     1141 aagactcatg agattcatta taaaaaactg gccgtttatg tacctgtgga tcatgcacag
     1201 aaaatgcgtg aagtattagc tgcagctggt gctggtacac aaggtgatta taccggtaca
     1261 agttttacct cgattggaca cggtcgattt actccggaag ctggtgcaca accagcgatt
     1321 gggaaagtag ggaagacaga acaagttcaa gaggcaaaag ttgaggttat tttgccggaa
     1381 actatcgaaa aacaagtgat tcaagccatg cggtcagctc atccttatga ggaacctgct
     1441 tatgatcttt tcgctataga tgaaccagtc gaaatgtttg ggttaggacg agtaggtgaa
     1501 ttgccgcaag aaatttctat agaggcattt gtagaacaag taaaagaagc gttccaacta
     1561 gatggactga gaatagtcca gccaaaaaat gcaaaatcct ctgtgaagcg cattgcgatt
     1621 tgcggtggaa gcggtgaaaa attttatccg caagcaatcg cccaaagagc agatgtctat
     1681 atcacgggag atatctatta tcatacggct catgatatgc aaagcgctgg gttgattgca
     1741 attgatcccg gtcattatat tgaatcgtta tgcaagcaac gatttatcga gaaatttgaa
     1801 tcttggaaac aagaagaaaa ttgggatatt aactttttcg tttctgaaac aaatacaaac
     1861 ccatttcaat ttaattaaaa ggagagaata ctatgtacga aaatcttttg ccacgttttt
     1921 tacgatatgt caaaacagaa acacgctccg atgcaacaag caaaacgact ccatcgactc
     1981 agacacaagt tgcttttgcg caagaattga aaaaagaatt agaagagtta ggcttagctg
     2041 atgttttcta taatgaagat aacggattcg ttatgggaac attaccaagc aatgttgatc
     2101 atgaagtgcg ttccattgga tttatttccc acatggatac agcagatttt aatgcagtag
     2161 gagtcaatcc acaaatcatt gaaaactatg atggcgagtc tacgatcgta ctagatgcgg
     2221 aagaacgcta tacattgaat acaaaagatt ttccaaattt gaaaaattat gaaggccaca
     2281 cattgatcac aacagatggc agtacattac tgggagcaga tgacaaatca gggattgcgg
     2341 aaatcatgac tgcaatggag atcttgatca ataatccttc tatccctcac ggtaccatca
     2401 aagtggcttt tggtcctgat gaagaaatcg gtgtcggagc ggataaattc gatgttgagc
     2461 aattcgatgt agattttgct tatacagtcg atggtggtcc tttaggtgag ttgcagtatg
     2521 aaacgtttag tgctgctcaa gcaaatatta cgatccaagg gaaaaatgta catccaggga
     2581 cggcaaaaga tacaatgatc aatgctctac agttagcaat cgattttcat aatcagcttc
     2641 cagccgatga agttccagaa aaaacggaag gatacgaagg tttcttccat ttgatggcat
     2701 tggatgggaa tcctgaagaa gcttctatga gttatatcat tcgcgatcat gatcgtgaaa
     2761 aattcgaagc acgcaaagca caaatcacac atatccaaga agagttgaat cagcgatttg
     2821 atcaagaacg aatcaaagta gatatgtatg atcaatatta caacatgaaa gaaatcattg
     2881 aaaaagatat gagcatcgtg gaattagcga aacaagcaat gatcgagctt ggtatctctc
     2941 cagtgatcga accagttcgt ggaggaacag atggttcaaa aatctcttac atgggtattc
     3001 cgacgccaaa tctatttgcc ggaggagaaa atatgcacgg acgattcgaa tttgtttctt
     3061 tacaagtaat ggaaaaagca acagacgtga ttgtaaaaat tgcagaattg aacacaaaga
     3121 gttaaaatcg aaaaagaaca gcttatgaca aggcgttcgt cccaatgatg gaatgtcatc
     3181 tgtctaaggc taaagggcag gacaatggaa ttggttgtcc cgccctcttt tttgaagagt
     3241 aaataggagg taaagaatgg aaaagctgct agttttttat aacaaaagtt cagggaaaga
     3301 tgagggtgag aaattagcac gctggtttca agattatgta aaagaaaagc agccagaatt
     3361 atatgtagaa ctagctgaaa ctggcccatc gattgatgat gcggaattag tcaagaaagc
     3421 agaagccata gaagctgata ctttagtaat tataggtgga gacggcacga ttcatcatat
     3481 cgtccaagcg ttccaaaaaa cattatctca ataccatgtc ggattacttc caggtggaac
     3541 tgtcaacaac cttgcacgtg ttcttgagat tcccctcaaa aaagaagaag ctgctgacgt
     3601 gatcctggaa ggcaatgtac ggaaaataga ctatggtcaa gtcaatgatg aagtcattat
     3661 ctctacactg acaatcggaa tcttagcaga tacagcagtt tggatcagtc aagaagaaaa
     3721 acagcgttac ggctcgtgga tctttatcaa gaagtttttt aagttattgc tgaagaagaa
     3781 aaaatatcat ttggatttag aaacagacga ggagcattgg aaaggaaaat cccaactgtt
     3841 gacagtcacg atgtctaact ccgtgggcgg ttttacaaat tttgacgact cagcgaccct
     3901 agatgatgga aaatttcatg taacgatcgt accttcctta gatattttcc gatttatttt
     3961 ttatttgcct agaatcatga gaggcaaatt ctatacgatc cccggcatta cttatttcac
     4021 agccaaacgc atgaaaatcc aagcgcagga aaaatctgtg ggcactcgaa cggatggaga
     4081 cacgacagat gatcttccag ttgaattatc tgttgtatca aaaggattaa cagtttattc
     4141 accaaagaaa gaagaaaaag cttagaaata gtagttaaca gaaagttgga agaagagtaa
     4201 tggatgtatt gttacacaat cctttgtggt tttacctttt cattgtgaat ttttacttat
     4261 tttgtctgat ggggtatgat aaacatcagg ctagaactag cggctggcgt gttcctgaga
     4321 ataatttgat atttgtcagc ctgttaggcg gtggtttggg aggattgatc tctcgaaaag
     4381 ttttccatca taaaactcgg aaaaagaagt ttactatagg attcgctgca ggtgtacttg
     4441 ttgatttgat tctcatttat tttttccatt aaggagcagg agatgtcgtc taaaggaacg
     4501 aaaactaaaa tcattctgaa tctgcttttg ctgattgtga tttttgccat tatttattat
     4561 ttaatcaatc aatcatttgc agatatcttt gccgaattgc tgtctaccag tgttgttgtt
     4621 ttggcagcgg ctgttatatt agggacagtt taccaaatcg ctgaagggcg ctcaatcaaa
     4681 gaaattgctg aacctttcgc gccgaatttt acaacgaaag atgggttctt gacctctagc
     4741 tatattgcgt tttaccgaat cgtttcgttt ggtacaggaa cgctgatctc ggagatttat
     4801 ttttataaca aaaaaggaat gaagctatcc cagggagcag gggtgacagc actccatatg
     4861 attatgtaca aactggcagt gatcacttat gcattagctg gcttggtcat ccaattctct
     4921 cttttttatt ctaatggtcc atcaatgatc tggtttatca tcgctggtat ccttttgacg
     4981 atcgtgattg ttgccttttt attggcgatg tccgtcagct tgaatgttca aatatttttt
     5041 gtcagcttat cgaataagtt atttaaaagt gagcgtttgc ggaaatgggt ggatacatgc
     5101 aatcagcaga tctactcttt aagggaaacg gtccaaacca ttatccaaga caggactgcg
     5161 ctattgcgga tttatctatg gaatatgtta aaacttgtat tttggtatgt acttccttat
     5221 ttgattctga ttgaaaacca tcctaaaata gattttctac tgaccatttc gtttatcagc
     5281 tttgcagtag tgctttcagg ggttattcct acgcctgctg gtattggttc ctttgaattt
     5341 gtctatttgt tattattcag accagttgtt ggaacagttg acgcagtttc ttcgctttta
     5401 ctgtatcgtt tcagttcctt tatcttgcca tttctctatg gatttgtgta tgtgattgct
     5461 gaacggcgaa gagtgatcaa agaagaaata atagaagtaa aaaaagagaa taaagaaaat
     5521 caagggtagg tcttttataa gatctaccct ttttatcaaa tcaaagaaaa acgttatgga
     5581 taagataatt ttacgtttgt ttggctcttt ttttatatac atacaatcaa ttttgttttt
     5641 ttaagattta tataagtcga gaaaaaaagt caaaaaacta tgtgtatatt ttgaccaaaa
     5701 ttgaccaaag cgatataata gtttttgtaa agatcaaaga aataagtgat cttaccttta
     5761 attaggaggg tatacctgta tgaatattga gaaaatgact acgacgcttc aagaagcgat
     5821 tgctgaagct caacagattg cggtcactcg aaaacatcaa gatatcgaca ttgcccatgt
     5881 atggaaaatc tttttacaat ctaatcattt tggtcgaaat ttctatacgg atgccggatt
     5941 ggatgtcgaa tcttttgaac atgaaattga tcgtctactg gatgagtatc cagttgtttc
     6001 tggtggaaac gtccaatatg gacaaaacct cagccagaat ttctttcgat tattgaatga
     6061 agcagaccaa atacgtgaaa gcttcggcga cgagtttctt tcaacggaag tcgttatttt
     6121 agcgttgatg aaattaaaaa attatccgtt aactgtttat ttgaataaaa acggattatc
     6181 tgaaaaagaa ttgcgtaaaa acatcgaaga gatgagagga ggagaaagag tgacctcaca
     6241 aaaccaagaa gaacaataca aagcattaga aaaatacggc gttgatttag tacagcaagt
     6301 caaaagcgga aaaatggatc caatcatcgg tcgtgatgaa gaaatccgtg atgtcatccg
     6361 tattctttca cgaaaaacaa aaaataatcc tgttctgata ggagaacccg gtgtagggaa
     6421 aacggcaatc gttgaagggc ttgctcaacg tatcgtacgc aaagatgtgc ctgaaaattt
     6481 aaaagataaa acgatttttt cattagatat gggtgcattg atcgctggag caaaattccg
     6541 tggtgaattt gaagaaagac taaaagcagt cttgaaagaa gtcaaaaaaa gtgatggacg
     6601 tatcctcttg ttcatcgatg aaatccacaa tatcgtaggg gcaggaaaaa cagaaggaag
     6661 tatggatgcc ggaaatctgt tgaaaccaat gcttgctcgc ggtgaattac atttgatcgg
     6721 tgccacaact ttagatgagt accgtcaata tatggaaaaa gacaaagcgt tggaacgtcg
     6781 ctttcaaaag gtattagtca aagaaccaac agtggaagat acgatcagta ttctgcgtgg
     6841 attaaaagaa cgttttgaga tccaccatgg tgttaacatc catgacaacg cattagtagc
     6901 agcagctact ttatctgacc gttatattac tgatcggttc ttaccggaca aagcaatcga
     6961 tttgatcgat gaagccagtg caacgattcg ggtagaaatg aactctatgc caacggaatt
     7021 agatcaagtt actagacgtt tgatgcagtt ggaaatcgaa gaagctgcac tgaaaaaaga
     7081 atcagatgat gcaagtaaaa aacgcttgaa aaatttgcaa gaagaattag ctgaacttcg
     7141 tgaagaagca aatgcgatga agatgcagtg ggaaacagaa aaagaagaag tcaattctgt
     7201 ttcagcaaaa cgtgctgaaa tcgataaagc aaaacacgaa ttagaagatg cagaaaataa
     7261 ctatgattta gaacgcgctg ctgtattgcg tcatggtacg atacctcaat tagaaaaaga
     7321 attgaaggaa ttagaagcta aagcaaaaga cagcgagatt aagatggttc aagagtccgt
     7381 taccgaaaat gagattgctc aagtcgttgg cagattaacg ggtatccccg taacgaaact
     7441 tgtagaaggt gaacgagaaa aactaattaa attgaatgaa acacttcata aacgagtgat
     7501 tggtcaagat gaagcagttg atgcggtcag tgacgcggta attcgttcac gggctggatt
     7561 gcaagaccca aatcgtcctc taggttcttt ccttttccta ggaccaactg gtgtcggaaa
     7621 aaccgaatta gcgaaagcgt tagctgagaa cttgtttgac tcagaagatc atatggtacg
     7681 gatcgatatg agtgaatata tggaaaaaca tgctgtgtct cgtctagtcg gagctcctcc
     7741 aggctatgta ggttacgaag aaggtggtca attaacggaa gctgtcagac gaaatcctta
     7801 tacaatcgtc ttgttggatg aaatcgaaaa agcgcatccc gatgtcttca atatcttgtt
     7861 acaagtccta gatgacggac gcttgactga ttcaaaaggg cgagtagtag atttcaaaaa
     7921 tacggttctg atcatgacca gcaatatcgg ttcacagtta ctgctggaag gcgtgactgc
     7981 tgatggaacg attccagaag cagtggctga acaagtcaac acgctattac gtggaaactt
     8041 caaaccagaa ttcttgaacc gaatcgacga cacgatttta tttacaccat tgagcctaga
     8101 caacgtgaaa ggaatcgttg acaaaatggt ggcgcaattg gcacaacgtc tggaacatca
     8161 agaaatcctg ctgacaatca gtgatgaagc caaaacgtgg attgctgaaa atgcttatga
     8221 accagcatat ggtgcaagac cattgaaacg atttatcact aaggaagtag aaacaccact
     8281 agctaaagaa atcgttgcag gacatgttat gcctaaatca aaagtaacga ttactttact
     8341 tgatggacaa cttcatttca aaacagaaga gctagaagaa atcgtctaat cttccattga
     8401 gactataaaa gaaaaaaggg gtctgacaca actagtgtca gacctccttt tttcaaataa
     8461 acagtgttcc agaagtagtt tcttcgaaaa ataagccgaa atttcttgaa gctgcccgct
     8521 tctgtcacaa cctcttttat taggtatttt tcactttttc taatttataa ctttcaaagt
     8581 aacgctcatc ttttttactt tgagaccatg caaacaatcc cattaaggca gcacctatcg
     8641 tatttgtcag caagtcgccc atcgtatcca tcaatgctgc tcgtccgaca aacagtgtgc
     8701 catcagaagc agcaaaacgt tgaagattca tgcctaagaa ctggtcgcat aagaattccc
     8761 agaactccca aaacacgccg caaagtccag caaacgcaaa gccgaataag aggaacaacc
     8821 aaggactggt ttttgatatc tcagcatctt tcatcaagta accaatcaat ccatagccaa
     8881 gagctgtcag gaccattgga ctcaccgcat gtaagatttt gtcccaaaaa gagatgatac
     8941 tgatcaaatg catccctgtg cctaaaaaga cagagatgaa caagaaaaac cagtaaaaca
     9001 aaacgattgc tgctggtatt ttaagtttga aaactttcaa aatgacttgt ggcatgaaaa
     9061 tcaatgcaat ccctaataaa gattccacga taaatgtgat tccttgagaa gtcgaatatt
     9121 tttgtgtgat aaattcataa aaattatagc agacaacgaa tacaccaaaa gcaagtaaag
     9181 ctatttgcac ctttttgatt tctttaatca ctgaaagtcc ctccatctaa aaatcttttt
     9241 acttctgaaa gcaactcaat ttctgaagaa accacttttc cgttcaattt gatcaaacct
     9301 attgtatata agttcaggta gtgaaattga ttttcagctg tttcttgtag tgcagcgatt
     9361 tttttcgggt gggttccgcc ttgctgtcga gtatcagtat ataagccgat cactgggatg
     9421 cctttcgcat aagccacacc gatttccgat gctaccccgg catcgatcgt cagcccatct
     9481 agtaaagcaa tcattaaatc actagccaac actttttctg tgtcagcaag agcaatcatt
     9541 cgactgtctg cataagcagt tttgtcattg attgcttcat tttcttgagg aagatagatc
     9601 gaaagctcag aagagagctc tctgatttct ttaactaact gttggttata taacaaatca
     9661 cttttagcaa acattggtgc ggcaaaataa attttcatct aaacatcatc ctttttatca
     9721 aaaataatcg ctaaagtaag cttccatgtt tggaatgata cgctctagtg acttcagtgt
     9781 ttcttcatct gtttctaacc gttcgattct tgccaaataa gaagcacggc gataattcat
     9841 catcaagcaa gtcaatgttt ggatatccag tcgaacggat tttcctatag gttcatttaa
     9901 gacacggact tgtccctgtt catcccatgt taaagcgaag acaccattat tccaaggagc
     9961 gacaggatct tcaatgatga aatgaaaagc atcagctgtt ccaacaaacg gaaaccgttg
    10021 aaggaattct tttacatcaa cgatccgagc cataaaatat ggttcgatct gctctttgat
    10081 ctgactatct tcaatcaaaa atgctaaagg ctcattttta taaatatctc catgtaccca
    10141 gtagatcatg gaaaaatgtg cggaaataaa gttccataag ccatttcgag cttcttgatc
    10201 aagatagaac atttctttta cgtgaaatat ttcttcagct acccagtaga acagaacacc
    10261 tcttggttca tggttgctat cgtagtagac agcggctgtg cgctcatctt cattttcgaa
    10321 acgccaatac tcttcccaat gaaaatcact gcggaataaa gcaccatggt tttgttgggc
    10381 aaatttgtca taaacggaaa atacgtctgg atgatcaact ggtaatcgct caaccatacc
    10441 acttactggg atttgtttag gtagctgtgt atctctgatt ttaaaggaaa gtttatcaga
    10501 catgatttcc catcctttac gccgatagta ggggatactg taaggataca aataagagat
    10561 ccattgctta ttcttgcgca tattatccaa tgcaacgata attagatctt gcatcaatcc
    10621 atggttagca tattctggat aagttccaac tccagtcaca ccacccattt tataacgtgc
    10681 accgtgaata ttgacttcac aaggatagat agcgatttga gagatcagtt tattttcgtg
    10741 gaaccatccg aacactttcg atacttccaa aatcggtttt ttggaacgga taaaagctcg
    10801 tttattctca aacccgcttt cctcgatgtc tgcttcagtc acttggaaca cataggaaag
    10861 caattcattg aattgatcga tatgttcttg agtgaccggt tttaattcaa gattttctct
    10921 aaaatctttt tgatccattt tttcatctcc atttattagc ttgataacag tatagcaaaa
    10981 ttctacaaaa aaaacttcta tagctgacta attaaaaact ctttaaatta ctgcttttcg
    11041 ttggtataat ggaaagtgct gattatatga aaagaggaga cgctatggat ataaagaaaa
    11101 tgaaagaacg ccaagagcag attcgtaatt tttcgatcat cgctcatatc gaccatggga
    11161 aatctacatt ggccgatcgt attttagaaa tgacgcacac tgtgacgtct cgcgaaatgc
    11221 aagatcagct gttagactcg atggatttgg aacgtgaacg tggaatcacg attaaattaa
    11281 atgctgtaga actcaattac acagcgaaaa atggagaaac atatatcttt cacttgattg
    11341 acaccccagg gcacgtcgac ttcacctatg aagtctctcg aagccttgct gcctgtgagg
    11401 gtgccgtttt agtggtcgat gcagcacaag gaatcgaagc acaaacattg gccaatgttt
    11461 atttagcgtt agacaatgat ttggagatcc tgccagtcat caataaaatc gacctacccg
    11521 cagcagatcc cgaacgtgtc cgtcaagaaa tcgaagacgt aatcggaatc gatgctagtg
    11581 aagctgtatt agccagtgcc aaatcaggga ttgggattga agatattctc gagcagatcg
    11641 tggaatatgt tcctgcacca agtggagact tagatgcacc gctaaaagct ttgatctttg
    11701 actctattta tgatagttac cgcggtgttg ttctaaatgt ccggatcacg gacggtatgg
    11761 tcaaacccgg agacaaaatc aaactgatga gtaatggcaa aacatttgat gttacagagg
    11821 tcggggtctt ctcgccaaaa gcagttgcta gagacttttt gatggtcggt gatgtcggtt
    11881 atatcacagc aagtatcaaa actgtccaag atacacgagt aggggataca gtaacgttag
    11941 cagacaatcc tgccgctgaa gctttaccag gttaccgaaa aatgaatccg atggtttatt
    12001 gtggtcttta tccaattgat acttctcgtt acaacgattt gcgggaagct ttagaaaaac
    12061 tgcaattgaa tgatgctgct ttacaattcg aaccagaaac ctctcaagca ttaggttttg
    12121 ggttccgctg tggattcctc ggacttcttc atatggatgt cgtccaagaa cgcttggaac
    12181 gggaattcaa tctagaattg atcacgactg caccatccgt tatttaccat gttaataaaa
    12241 cggatggtac gactgctaca gtagataacc cggcagattt tcctgaacct gtaacgattc
    12301 aggatgtaga agaaccattc gtcaaagcac agatcatggt cccaaatgat tttgtcggtg
    12361 cagtaatgga actttctcaa cgaaaacggg gagaattcat tacgatggat tatttagatg
    12421 attaccgagt aaatgtcgtc tataatattc cgctttctga aattgtcttt gactttttcg
    12481 acaaattgaa atcaagcacg aaaggctatg cctcattgga ttatgaaatg tctggttatc
    12541 aaaaaagcaa gttagtgaag atggacatct tgctgaatgg agaaaaagtc gatgcattaa
    12601 gctttatcgt tcaccgagac tttgcctatg aacgtggaaa agcaatcgtt gaaaaactga
    12661 aaaaactaat cccacgccaa caatttgagg tgccaatcca agcagctatt ggacaaaaaa
    12721 tcgttgcccg ttcagacatc aaagccttgc gtaaaaacgt cttggctaaa tgttatggtg
    12781 gagatgtttc tcgtaaacgt aaactcttag agaaacaaaa agaagggaag aagcggatga
    12841 aacaaatcgg ctccgtcgaa gttccgcaag aagccttcat ggccgttctg aaaatggacg
    12901 aggacgaacc gaagaaatag caagggtttg tttgaaaaca gtgtaaaaaa taaaccatgt
    12961 ttacatggat ttgccaccgg tttgccaccg gtg
//
LOCUS       DAFCNS010000076        12434 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFCNS010000076 DAFCNS010000000
VERSION     DAFCNS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.078"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..452)
                     /locus_tag="LNE23_002240"
     CDS             complement(<1..452)
                     /locus_tag="LNE23_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6932070.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLIS"
     gene            complement(449..1102)
                     /locus_tag="LNE23_002241"
     CDS             complement(449..1102)
                     /locus_tag="LNE23_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6932071.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1390..1910
                     /locus_tag="LNE23_002242"
                     /pseudo
     CDS             1390..1910
                     /locus_tag="LNE23_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1812..2528)
                     /locus_tag="LNE23_002243"
     CDS             complement(1812..2528)
                     /locus_tag="LNE23_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBK6932072.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(2652..2837)
                     /locus_tag="LNE23_002244"
     CDS             complement(2652..2837)
                     /locus_tag="LNE23_002244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932073.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3064..4337
                     /locus_tag="LNE23_002245"
                     /pseudo
     CDS             3064..4337
                     /locus_tag="LNE23_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4444..5475)
                     /locus_tag="LNE23_002246"
     CDS             complement(4444..5475)
                     /locus_tag="LNE23_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6932074.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5482..6312)
                     /locus_tag="LNE23_002247"
     CDS             complement(5482..6312)
                     /locus_tag="LNE23_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6932075.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6309..7124)
                     /locus_tag="LNE23_002248"
     CDS             complement(6309..7124)
                     /locus_tag="LNE23_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK6932076.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7121..7945)
                     /locus_tag="LNE23_002249"
     CDS             complement(7121..7945)
                     /locus_tag="LNE23_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBK6932077.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7935..9035)
                     /locus_tag="LNE23_002250"
     CDS             complement(7935..9035)
                     /locus_tag="LNE23_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6932078.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9213..9998
                     /locus_tag="LNE23_002251"
     CDS             9213..9998
                     /locus_tag="LNE23_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBK6932079.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10031..10414
                     /locus_tag="LNE23_002252"
     CDS             10031..10414
                     /locus_tag="LNE23_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK6932080.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10490..10621
                     /locus_tag="LNE23_002253"
     CDS             10490..10621
                     /locus_tag="LNE23_002253"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6932081.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10590..10826
                     /locus_tag="LNE23_002254"
     CDS             10590..10826
                     /locus_tag="LNE23_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6932082.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(10904..11875)
                     /locus_tag="LNE23_002255"
     CDS             complement(10904..11875)
                     /locus_tag="LNE23_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBK6932083.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3737 a         2789 c         2242 g         3666 t
ORIGIN      
        1 gatattaata aagaatatgg taaaaattta aggcttaaat ctttcgcatc tttttgcata
       61 tcagctgtag aaacaaattg tagatttgtt tttttaccat cattaagctt tatttctctt
      121 tcttcaatta tcaaagaatt actatcactt tctaaattaa catttatatt atctttgatt
      181 tgtatttcat tcttattatt tttttctttt atgaatactt ttatttcact actcttggaa
      241 taaaactcca aagtttgttc tatatatttt atttcttttc cattcatatt actagaaatt
      301 tcatttgcca tattatgaat cttttctttt ctcgtctcca aatatgtttt aggaaagata
      361 aaaaaaatta atgaatgaac taatataatt attattccaa gaacagaaaa tatttgtatg
      421 aacatttttg gaaatatctt taattttttc attttctctc caatttatat cctacatttc
      481 ttatagtagt tatacagtct aattgtaatt tttttctaag ttcttttata tatacatcta
      541 ttactcgatc ataaggagtt tcttcgctat ctttccatac ataatcaata atttgcattc
      601 ttgttaacac ttgtccatta ttatccaata aacattttaa tacttctagt tcttttgcat
      661 ttacatctat ttcttcacca tttattttag ctgtatagct attaaaatta accgatagat
      721 ccttatattc aaacttctct aaatgtccat aattcttctt aattaaagaa tctattctag
      781 cttttaagac cggcaatgaa aatggctttt ctacatatcc gtctactaaa ttagtaaatg
      841 catcaatttt atattcttca tcactaaatg cagtcaagat tagaattggt aaattgcttt
      901 tctttctaat ttctttcaac acttctaaac catttataaa aggtatctga atgtctaaga
      961 taaccaaatt tatatcgcta ttaaattttg acaaggcttc tcttccatct ttagcttgaa
     1021 taacagtata tccaaattct gaaagatatt cacttattcc ctctcttatc atattatcat
     1081 cttcaacagt taatattttc atatatacct ccacttaagc ctatacacta gatattatac
     1141 agcatattat ggctctataa tatctagtgt tggtgacgcc ttctgttgcc aatacttttt
     1201 ctttttttta ataaatgcaa acagacacct tgaaatcctt tagaaaaaag gcttctataa
     1261 tattttgtgt tgttggaaaa tcgaaggcga ttttctacta acactttttc ttcgtttcaa
     1321 ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca aaacatcacc ctaccaaagg
     1381 agtcgctcta tggataatca tactagaaaa ttactcggat taacagataa acatttattt
     1441 tttgatgaag aatggctgat tgaaaaagaa tataaaggcg ttcaagcgca gtttattaaa
     1501 gggagattgg atgactcacc ttcccattgt gaacactgtg gatcgattcg tattatccgc
     1561 aacggttcct atacgacacg tactcagatg ttgaaagtca aagaaaagtt gactatttta
     1621 gaattaaaac gtacccgttt tctatgctat gattgtgggc agaccttttc tgcgaaaacg
     1681 gacttagttg atgaacatca tcaactaaca aaggaactga aacaggcgat tctcatagaa
     1741 ctgtacgaaa atcaatcacg taatctgaaa atcagaagaa gtaccgtcgg ctcaagcgat
     1801 attggaaact cttactcaaa gattccacga cattggagcc gctcaaacgg cattatcatc
     1861 gactgtttaa acgaccaatt agtcaaacag aaatcgtcga tgaaccgttg tgcacaatgg
     1921 tgtcctgaac gtacagcgac accttccata tccagagcag tcgccacatc acggggatgg
     1981 atgccatcca aattaaaagc aaccacgcca gtatgatgtt caggatcttg cggtccgtaa
     2041 atggtgatac cgccgatcgc ttgtaattta ggtaaaacgt aggcaaccag ttcttgctca
     2101 taacgatgaa tattctccat accaatctca ttcaagtaat cgattgctcc tgcaatgttc
     2161 ggagtaccag cttcaaattt ccatggcagc tctttccatg aactatcgta aagattgaca
     2221 aaatctatca tttcaccgcc gaattcgact ggctccattt gttctaacaa ttgacgtttg
     2281 ccatataata caccgattcc tgtcggcccg cacattttat gcccgctaaa ggcgaaaaaa
     2341 tctgcatcca acgcttgtac atcaactgtc atatgcggtg ctgcttgggc accgactgga
     2401 ctatcctcaa ctacaacagc ctgttcaacc ctcagccgtt tcaagcacaa caaataacct
     2461 tctggatctg gtttgccgac ctgaagatca tcgatcgtca caaaggaatc aattttttcc
     2521 ttaatcatcg gatgtgcttc aaaaaaatag tctagctccc gcagttcaga attgagacta
     2581 ctccgatttt ccagtgctgc tgatccgctg ctcaaaatgt tcagcatcca ctggtctccc
     2641 aaccgcttga cttatccctt cataaaagga cagttctgct gccgtcccaa caccttgttg
     2701 atagtctgca aaaggaattt tatagccgaa ctgctccagt aaataggctt cagtaaatag
     2761 tgatgaatac tctcgtaaaa catccattca ctgtcaatca tcgtcccatc aaaatcaaaa
     2821 ataagtgctt tggtcatctt ctttccccct tacccaaaac aagcaaaagc tgtgtcctgc
     2881 tctctctaca gtatcagcat aaaaactgcc gtctgaaaag tcaaaggtga aagaaatgtg
     2941 gcgctataat ttctaggact gatagtaaat cattctatca gtccttttga gcataaaaaa
     3001 acaaaacata cagaagctcc gttagaattg aatcaccaca acacaatcaa aggagcgttc
     3061 tgtatgcctg atcttcatct tatcaaacta ttactaggaa tcaaagatgg aaacattcaa
     3121 atcactcatg tcgagaagaa aaaaatacat tcaatcaaca gtatcgttgt ttctgccacg
     3181 ctcattaaaa cgattcagcc ctgtcctcag tgtaaacagg tgaatcaaca tgggctgatc
     3241 gttaagaacg gaaagaaaaa atcgctggtc cttctcaata agtgtgcgaa tcaacggact
     3301 tatcttgctt tggcaaagca acggtacctc tgtcgtggct gtcacaccta ttttacggcg
     3361 aatacctaca ttgttgaaaa aaactgtttt atcgccaaac aagtacatta taaaatattg
     3421 gaggaattga ctgaaaaaca agcaatgacg acgatttcta aacattgcgg cgtttcttgg
     3481 tcaaccgttt cgcgaacact cgcttctctg ctccccatga caaacgtgaa aataaattgg
     3541 ttgcctcgct gttcattggt agatgaattt cgttcattga aaaatcaagt cggtccctat
     3601 tctttttctt gtatggatgg cgatactggg aaattactgg atattcttcc ttcacgtaag
     3661 aagaaagatc tcatttctta ttttatgcaa tttgaacgac gtgcacgttt aaacgtaaaa
     3721 atactcgtaa cagatatgaa cgcctcttat gctttgctga taaaagagtg ctttcctcat
     3781 gccaaactcg ttgtggatcg ctttcacatc gtcaagcacc tcattcgcag ttttaaggat
     3841 atttgactat gagtcatgaa aagcttcgac cgaaacgatc caatacaagc caaacactat
     3901 cgacaagtga aggctttatc tcgacttctg atcaaacgac aggacacgct tgtttatgat
     3961 aaatggacaa aatggcgtaa ttttggttgg gcctatttaa ccgaaagtga agtcgtagaa
     4021 cgtctacttt ctacttctga tgaactacga atagcttatg cttactatca agaaattctt
     4081 caagcttttt atgacaaaga agcggacatc ttctttcagc ttgtaaaaac aatgcccaag
     4141 tcggttccaa gagaactgca tcatataaaa aaagcgttta taaactatga atccggcatt
     4201 cgtttagcct tagagttgcc ttactcaaat ggcaaaatag aaaatctgca tacccacatc
     4261 aaagcattga aacgaatagc ttacgggtta ggatgagaac cagaattttc ttaatcaatc
     4321 atctgatcac ttattaaaca taaaaaaaca tctaaagcag aaaatcaatt ctaccttaga
     4381 tgttcttata tcactctatc agtcctattt gacaaagagc cagaaatgtt tgccgactat
     4441 acactagctt tgttgtgcaa aatgctgagt atcaatttcc acagggattg ctagaatatc
     4501 tttcagctga atgtataagg catcgctaat tttctcttcc cgatcgttca accgatccac
     4561 taaaatttct ttgccctcca gcgtttgaaa aacataccgt tgaatgttgc ctaaaataaa
     4621 tgattccttc catagaaccg gaatcttaac cccctgagga accggttcaa attgaatcag
     4681 ctccggtctt aaataataaa tgcagcgatt gcccttagcg gaaaactcgt gggtatactg
     4741 attcagctca taccattcga aacgattata gttgccgata aattccgcaa caaatcggct
     4801 ctgaggattc tgatacaatt ccttcgaggt ggaaacctga gccacccgcc cagcttccat
     4861 cacaaaaatc cgatcactga ttcgcatcgc ctcttcttgg tcatgagtca caaaaatcat
     4921 cgtcatccct agcgattgct ggatagcgcg caaatcccgc tgcagctgtt ttctaattcg
     4981 ggcatccaaa gcacttaatg gctcatccag cagcaggact ttcggttcag tgaccaatga
     5041 gcgagccagt gccacccgtt gtcgttgacc gccagaaaga aactggacat ttttatctgc
     5101 ctgctccgtc atatccacca atgatagcat ctcaaaaacc ttttcctgaa tcacctcagg
     5161 actcagcttc tttaccttca aaccaaaggc tacattttca aaaacagtca tagttggaaa
     5221 caatgcatac tgttgaaaaa caaagccgat attgcgatcc ttcgtaggct tatcggcaat
     5281 attctcattg tccagcacaa tctcaccgct ctttggttgt tccaatcccg tgatacagcg
     5341 cagcaacgta gactttccgc acccgctggg acctagtaaa gtaaccagct cacccttttc
     5401 aatctgaatt tcatcaatag ctaaaatctg tttttcatca aaaaacagat tgagatcatg
     5461 tatttctaaa aacattttcc gtcacaactc ctttaagggc ttctttttcg actgcttctc
     5521 ttccctcgtc tgcttaaggg acagctgttg cttttgtcga taagtcagca taaaggtagt
     5581 taaagcaatt cccaccataa acagcaaata caaaaccatc acagcgctag ccaaatggcc
     5641 gttttcgttc atccgccgca gcatatagat gcgtaccgtc tgaaatcgac cgccaatcaa
     5701 caggttcgtc agcatgtact ccccaaaaac cgaagaaaaa atcatcagag aaactagggt
     5761 cacaccgata cggatgtttg gaaacagcac ctggataata atcgtagaca tcggggcgcc
     5821 caaggtttct gctgcatcca ctaattcctt cagattaatt aagcgcatct ggttgttaag
     5881 actaagatag acaatcggca acgccgtaat aaacaatgca cctaccaaca ctacaaacat
     5941 tgggatacca gtttcagcat acgttcgaag caaagccgtt accaaaatca ctcctggtaa
     6001 agcatacggc agcaggagca gcttttccag ccaacgattc aaacgaggga aatacaaatg
     6061 aatccaaata atggtgggaa tcatcaacaa cagaatcgtc accaagacca cacctgctaa
     6121 taaaagtgag cgacccaccg cagcaataaa ttccctgtcc gtcagcagct gttggaacca
     6181 ttgcaaggta aaatcactag gtaaaagcga ttttgaccat ttagtggagc tggcatacaa
     6241 aatcgttcca ataatcggca gcaagtaata caggacaaag cagaacaaaa tcggttttat
     6301 taactttttc acgtctgcac caccttgcct ttcttttgaa taatcaacat tgccgttacc
     6361 agcaggacac ctaaagcgac tgccaacgca ctgcctaaat taggcttggc gaacacatct
     6421 cccgacacta aagccgcaat tcgaatcgtt aaaagattta cattactgct ggtcagggca
     6481 tacgctgttt cataggtccc catgccatta gcaaataaga tgacaaaggt gctggcaatc
     6541 gatggtcgca aaaaaggcaa agccaccttg aaccaagcat acgctgatga agcgcccagc
     6601 aattgtgcgg actccagcca ttcctttttc acttccttca tgctgggata caaaaacaag
     6661 acccctaacg gaatttggaa ataggtatac gtgatggtca atccccacca agaatacaaa
     6721 ttgaagtccg ctagaaatgg caaaatcatt ttcagcacac ctacattccc caacaaaata
     6781 ataaaagaga atgccagcgg aatcccagca aaattagctg ccaaattgga aacaaaggtg
     6841 attttctcct gcaatttagt cgatagattc attaagcata aacataaaat aaacgtcccg
     6901 attaacccaa tcacactgga gctgacagca atcagtacgc tgttaacaaa ggcctgtgtg
     6961 taatatttgt tgctgaaaat atcactgtaa ttggccaatg tccactggtt agttaaatca
     7021 ctgtgcaagc tgcacagaac cataaaaagc agcggcagca gtaaaaatgc acagaagata
     7081 aaggaggcag gaaccagaac caagcgtttt ttcatctctt tcatcccctc atcaattctt
     7141 gaatgtcctg cattttctct gacggtgcaa ttcctaataa ccagcaaaac aatggagccg
     7201 ccagcagctg tggtacagtt tgttccttca gcttttggcg tttatttgaa atcaaataaa
     7261 aaggaacttc acgatgtgca gccaagctgc cgccatgcag tccgtgcgca tccatcccgt
     7321 gatccgcagt caccacgact tgataacctt tttgcagcca ttcaggaagg taaatagcca
     7381 ataatctgtc cgcctgatta accgctgctg catattcatg agaatcggcg gtaaatttgt
     7441 gaccgacatc atcaatattc atcgaatgta cgaccaaaaa gtctggctga tactgattca
     7501 ctaaataatg ggcatccgca aataaatgcg aatcagggta agtgtcctca ctataaaaga
     7561 tgccatgctg aatcagtcgc tcctcctgat gctggatgcg atcaaccaaa cgattatagg
     7621 gggcttgatt gtacaactcg ctgtaccagt ggtaagcggc ggctcctgtt tttccgccag
     7681 cttccttaac taattcaaac agcgaccact ctttggatcg ctgagcatgg taattactat
     7741 agatgccatt ttcataggct ggaacgccgg tcatcagtac ttcgtataac ggacgtgaat
     7801 tgctgggcag ctcacttttt accttcaata agcttgcttg ctggtgctca actaaatgat
     7861 tcatgaaccc aagctgggcg acggctgtgt cgtagcgaca gccgtctagt ataactacaa
     7921 tcagtttgtc aagtctactt tgcataggtc agtacctccg cctgccaaag ctctggaagc
     7981 tctacagagg tcttgttcca agcttcattg tcttcaactg gcaccgccgc ttcataagca
     8041 tcctccggca gtaatttttc agctacttct ttaggcagct ctaccttgcg aataggtcgc
     8101 gcatagccct ttgccaaatt gatttgccct tcatcagata aaatatattc ccgtgctagc
     8161 tttgccgcat tgggatgagg ggcattttta ttgattaagg ttgtataccc gctaatgatg
     8221 gagccgtctg ttggcacagt tacctcaaaa cgctcggagt caatttggtc acgataattt
     8281 agtccattga agtcccaaac aactgccaca tcgatttcac ctttttcaag atttgctacg
     8341 ctggcatcaa cggtggacaa acggcctttt tctgccattt ttttgtagta attcagacct
     8401 ggctgaatat ccttttcgtc acctccgttg gcaatcgccg cagccaatac agcaaattgt
     8461 gcttggttgg caatcgtcac atcccccacg ctaacttgat agtcaccctc ttgcagctcc
     8521 ttccagctag tcggtgcttg cttcacattc tctttatcgg tgataaagga aatggttccc
     8581 gtatagctta acaaccaatc cccatcatca tcctttgccc aatccggaat ttcctcccaa
     8641 tagttggtct tataaggtaa ggttaactct ttgctttcag ccaacggacc gaagctgata
     8701 ccgacatcac caatatcggc ggttccattt tttccttctg attcaaactt agccaattct
     8761 tctgcactgg acatatccgt atccacgtga cttaagccgt attctttttc aaggtcgttc
     8821 caagtgccta cccaatttgc ccaagtatcc ggcattccca agctggcaat ctctccttct
     8881 tccttggctt tttctataat cgtatctaaa gagtcgtctg ccgcagccga ttgcttcgtt
     8941 gcttcacctg agccgcagcc tgtcagcagg atacttactg ttgctgcaca aaataaaccg
     9001 atagtcatgt tacgaatttt ttcattcatt ttcattcatt cttctcccct ttcgataatt
     9061 ccattctaac aaagctttgt aatgagagaa tcagtttctt gaaaaggttt catgaacaat
     9121 gtaaaggttg tatatggttt gtaaatttcg acgaattccc gttaaccttc taaagctttt
     9181 gcggtatgat agtagaaaaa aggaggctcc ctatgcagta ttatgatcag catctccaca
     9241 cgtatttttc ctttgattca gaggaaaaat ttgaaaacta tttagcctat cagccggagt
     9301 ttttcgtttc tacggatcac tttgatttaa acaatccatc taccggaaaa gatgacattc
     9361 cggattatcc agcctatata aacaaactgg actctttaaa agaaacctat tccaccaaat
     9421 ttctgcgagg tatcgagatt ggcgttgttc caggacaaga agagcggatc gtggattacc
     9481 tcgctcagca tccttatgat ttaaagctgg taagtattca tcaaaatggc cgctttgatt
     9541 atatggacga tgtggtttta accaaagatc ccattcaaac taccaaggaa tattttgatc
     9601 agatgttgca ggtcttgacg gattttccag aaggagatat tttgactcac tttgattatg
     9661 gattacgccg cttcaccttc acagcggaag aattcgcgga gcattttgag gctcagctgc
     9721 taacgatttt caaaaaggtt gttgaacgag aattggcgat ggagttaaat gccaagagct
     9781 tcttgaaata cggcaacgaa gacctttacc gttatgccgt tcctttgtat caaagtgtcg
     9841 gcggaaagct ctttacctta ggctcggatg ctcatgttgc cgaagattat cagctgggct
     9901 ttaaaaaaat gagtcagctg ttggcagact gtcaggtaga aaagttgcta gtcattcaag
     9961 gaaaagaacg ccgcctgacg ccgctgccga ttttgtgatt cctatataat agaagaaagt
    10021 ggtgaaaaaa atgagcaacc tgtgggacta caatcaagag gcacccatcc attatttaat
    10081 tgcgcgacat tgggatgcgt tgaaaattga ggcggtttgc cgatccttgt tagcagccgt
    10141 tccaaaacaa caactggaaa attttttggt ggctgacagc ttgcagcggg aaaaagttca
    10201 ggcttatttt gcagccttta aagatcagcc tttggaatat ttgcatgcgc agtttcatct
    10261 tttttatcaa gtagccgccc cagatgacta caacgattta cgaggacagc tgcaattgac
    10321 ctttcaagcg gatgaaaccg cctataccgt tttattggga atggctcgcc ttggtgatca
    10381 ggcgaaggtc gagtggcgga ttttcgatat ttgatggcag gatcaaggcg tttcagttgc
    10441 aaaccttcct ctctttcaat tagaaaaacc gcagcaggaa gaagtttgtg tgctggtcac
    10501 cgatgctggt gaaattcgtc tccgcttatt tcctgaccaa gctcccctgg ctgtagccaa
    10561 ctggcgctcc ctagctgagc aaggcgttta tgacaacaca cctttcggct cgtgtcatta
    10621 aggaatttgt tattcaaggt ggcgctttgg atggttctgg tgatgaagct gtttccagct
    10681 atgagggctt tttcgctgat gaagtgcatc gcggacttta tcattttaat ggcgccttgg
    10741 ccttagggga ccacggcccc cacaccaacg gcaaccagtt ttttattgtg caaaacacaa
    10801 aggcgcaggc tgacctgcta atgtaaagca agcatacttg gagcttggag gactaccgga
    10861 gctggatggt cgttatactg tttttggaca ggtataccaa gggttagaaa tcgtagcagc
    10921 gatcgcagct cagccgacaa acagtgaaga tgtaccgatt gagccagtgt tcattcgaca
    10981 aattaacttc gaaaaaacat cgtaaaaaga cactcactga cttcagcaaa agctctattc
    11041 ttgagctggc tgcagctagt gagtgtcttt catttatcca taagataaag cttggattca
    11101 ataaataaat aaatgttttg gttaaagctg tgaccagcaa atgaggaacc gtgcgagcca
    11161 gttgatctgc caatgcaaac atttctttag gtgaaggcgc tggcgtgtct gccaacggat
    11221 actccagtcc caatgcttgg taaccgtgca taccatagca gtggtacggt agtaattcta
    11281 acggtacgaa ccgatcaaga tctgccacaa aacaagcaat cgtcagcagc tcggtcaggt
    11341 tcatattgat acctggaacc acaggaactc gaattcgcac ttcgcagtta ggatactcaa
    11401 ttaaaaagcg acgaatattc ttcaaaattt gttggttatc taagccggtc cattgtttgt
    11461 ggagcttgct ggtaaacatt tttacatcca caaacaatgt atccacatag ggagctacct
    11521 tttggatttc attataagca ccacaaaagc tggtttccag tacggtattg attccaatgt
    11581 atttcgattt ttgcaaaatt tctttagcaa aatccgcctg aaccagtgcc tcgcctccgc
    11641 taatggtcac tccgccatcc gagtgaaagt aaaaaacagc atccttctca atctcatcca
    11701 ttacctcttc agcggtcatg gtctggccaa atcctttttc aatctgcttg ctttgtgatt
    11761 cgggaatcga acaccaggca cagcgtaatg ggcagccctt taaataaaca acggtccgta
    11821 aaccagcacc atcatgtaga gaaccctgtt ctattcttaa tactgttcct tccatataga
    11881 tttttgaatt acacactagt ttaaactaaa acacacaagg aaaaacttac catttaatca
    11941 taaaattagc tcatataaat atactaggat gtaaaataga ctggtcaaaa tctggtcaaa
    12001 aaagaggact aatacaggta gtcctctttt ttgttatttt aatattctaa atcatttttt
    12061 attggtttat tcccttattc gatgtagact caaatcacta tatcactaga tatcaaacgg
    12121 cgtcatacgg aatcaaaaac atcaaatttg acctgttaaa tggtcattga ataacattca
    12181 aaatcaataa aacggagtca gacacggagt caaaaacgct gagagtaaaa aatatataca
    12241 gccttgtagg taaagtacca cagggctgta tattttttat ttcagattac cttaaatgat
    12301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
    12361 tcaataaact atttttggat acatggtatc tgaaacagct aaaaccttgt cattacagca
    12421 aaaaatcacg ttat
//
LOCUS       DAFCNS010000077        12432 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFCNS010000077 DAFCNS010000000
VERSION     DAFCNS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.079"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..190)
                     /locus_tag="LNE23_002256"
     CDS             complement(<1..190)
                     /locus_tag="LNE23_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932084.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="LNE23_002257"
     CDS             complement(200..1015)
                     /locus_tag="LNE23_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6932085.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="LNE23_002258"
     CDS             complement(1277..1720)
                     /locus_tag="LNE23_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBK6932086.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="LNE23_002259"
     CDS             complement(1854..2192)
                     /locus_tag="LNE23_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6932087.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="LNE23_002260"
     CDS             complement(2180..2557)
                     /locus_tag="LNE23_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6932088.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="LNE23_002261"
     CDS             2781..3974
                     /locus_tag="LNE23_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6932089.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="LNE23_002262"
     CDS             complement(4140..4562)
                     /locus_tag="LNE23_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6932090.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4957..5151
                     /locus_tag="LNE23_002263"
     CDS             4957..5151
                     /locus_tag="LNE23_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932091.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5148..5594
                     /locus_tag="LNE23_002264"
     CDS             5148..5594
                     /locus_tag="LNE23_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932092.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5804..6976)
                     /locus_tag="LNE23_002265"
     CDS             complement(5804..6976)
                     /locus_tag="LNE23_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6932093.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7182..7997)
                     /locus_tag="LNE23_002266"
     CDS             complement(7182..7997)
                     /locus_tag="LNE23_002266"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6932094.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8147..8593)
                     /locus_tag="LNE23_002267"
     CDS             complement(8147..8593)
                     /locus_tag="LNE23_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBK6932095.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8590..9594)
                     /locus_tag="LNE23_002268"
     CDS             complement(8590..9594)
                     /locus_tag="LNE23_002268"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK6932096.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9634..9963)
                     /locus_tag="LNE23_002269"
     CDS             complement(9634..9963)
                     /locus_tag="LNE23_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBK6932097.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10089..12416)
                     /locus_tag="LNE23_002270"
     CDS             complement(10089..12416)
                     /locus_tag="LNE23_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6932098.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3513 a         2430 c         2272 g         4217 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4741 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4801 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4861 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     4921 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     4981 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5041 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5101 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5161 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5221 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5281 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5341 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     5401 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5461 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5521 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5581 ccactttaag ctaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5641 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5701 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5761 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5821 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     5881 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     5941 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6001 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6061 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6121 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6181 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6241 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6301 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6361 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6421 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6481 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6541 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6601 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6661 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6721 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6781 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6841 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     6901 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     6961 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7021 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7081 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7141 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7201 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7261 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7321 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7381 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7441 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7501 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7561 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7621 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7681 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7741 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7801 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7861 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     7921 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     7981 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8041 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8101 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8161 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8221 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8281 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8341 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8401 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8461 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8521 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8581 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
     8641 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8701 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8761 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8821 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     8881 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     8941 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9001 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9061 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9121 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9181 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9241 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9301 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
     9361 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
     9421 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
     9481 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
     9541 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
     9601 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
     9661 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
     9721 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
     9781 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
     9841 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
     9901 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
     9961 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    10021 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    10081 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    10141 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    10201 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    10261 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    10321 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    10381 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    10441 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    10501 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    10561 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    10621 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca
    10681 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    10741 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    10801 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    10861 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    10921 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    10981 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    11041 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    11101 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    11161 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    11221 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    11281 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    11341 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    11401 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    11461 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    11521 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    11581 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    11641 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    11701 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    11761 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    11821 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    11881 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    11941 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    12001 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    12061 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    12121 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    12181 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    12241 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    12301 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    12361 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    12421 cctctcaata aa
//
LOCUS       DAFCNS010000078        11844 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFCNS010000078 DAFCNS010000000
VERSION     DAFCNS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.080"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..456)
                     /locus_tag="LNE23_002271"
     CDS             complement(37..456)
                     /locus_tag="LNE23_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HBK6932099.1"
                     /translation="MKKIIVATHYRLAEGFKSTLGYIAPNMVEIIAINAYLENTSVEN
                     QVQAALDQFEAEEQILVFTDLLGGSVNQTFAKFLDRYNIQLIAGVNLPILMTLALAIG
                     SNDLSETTIRQAIEESRQQIVYVNDLLQVSAMDDMDE"
     gene            complement(477..1307)
                     /locus_tag="LNE23_002272"
     CDS             complement(477..1307)
                     /locus_tag="LNE23_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6932100.1"
                     /translation="MNNERTKGLTQELNKKDRRNAAWRWSFIGSNNINYGTLQGTGYA
                     WSMAKTLRKIYPNDDDYVEAMRVEYEYFNITPYMGPLVAGADVALQEQQGTESLGAVR
                     SIKTSLMGPLSGIGDSLLWVLYPTIMGSISGYMALEGNPLGAIIWIILNLGLVWMRLK
                     LFDLGYESGIRLITDMADKLNAITESASVMGLTVVGSLIATVVKVYTPIEFTFGEVTM
                     GLQAEVFDRILPALLPVLLTALVYFLMEKKKWGFLKVIFLLLAFSLLGAYFGFLGVAP
                     "
     gene            complement(1300..2088)
                     /locus_tag="LNE23_002273"
     CDS             complement(1300..2088)
                     /locus_tag="LNE23_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6932101.1"
                     /translation="MPEFLQNILIILIPMLTLIDNRGITIINHWPVTVGLLAGLVMGD
                     LQSAMVIAGTFQLMSLGVAALGGSSVPDYGLATVVAIYLNARTGAEIGTSIAVGLPVG
                     ILAINLDIFSRTINSFVSEKSKQYLEERKFSKMQWINFISVFFVAMQAFIPMVILVAF
                     GQVAIETATQWIPEWITNGLNVAGGMLPVVGVAMLMRYMPTKRYIWALLAGFIMAAYM
                     ELPILAVSIVGLALAVFIYNNLTSQKVAVTTENAAYDEGDDFDE"
     gene            complement(2113..2586)
                     /locus_tag="LNE23_002274"
     CDS             complement(2113..2586)
                     /locus_tag="LNE23_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6932102.1"
                     /translation="MGLVLARIDERLVHGIVVTQWASAVKAKRIMVVDDQVAQDEPRK
                     AAMRLSKPAGTGMSLIDTETGITNFKAGKYDSHNVLLVVNNVKTLLDLSEQGIAIPKV
                     NIGIMLDRNDRKKLAKNFAASPEELQQLQQLADKGIPVSYQFAPSDKEEPLDKFI"
     gene            complement(2603..4660)
                     /locus_tag="LNE23_002275"
     CDS             complement(2603..4660)
                     /locus_tag="LNE23_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 31"
                     /protein_id="HBK6932103.1"
                     /translation="MLFKSKEVIVSEQSFVVKDRNQCLKVEGWGRDTVRVTCYPNHKG
                     FDPKQGIEELEKNVLTTATVTETSDQLILTNNRLTATYDGEKLVFYNNGTIILEEYSR
                     KQSQVRRTVGIDTHIPIPDEPTSSLNISPREFTYSGENTYQATLRFEGDPNEKLYGMG
                     GYQEENFNKNGGTYELMQRNSQTSIPFYLSDKQYGFIWNNAAIGEVTFGYNQKIWKSN
                     NTNLIDYLVTVGENPKQIIENYTQMTGRTPRLEPELLGLWQSKLRYQTLDELRTVYEG
                     YNQRGISLSVLVVDYYHWTSDGDFAFDSRYWEGIEDFAEEIKKTGTQLMVSLWPTVTE
                     ESRYYQSYRNNQLLIRSVTNKDSLFAGKALLDFFHPESKTFLRQVLDENYRSKGISLF
                     WADQAEPEMDVYQHKEYLIYEGSLEKYANKYPLHYLQAIQKNPERSNTEPTLIRSAWF
                     NAQKYGALAWSGDIESSFDSLRRQVQVGLSMGISGITWWTSDIGGFHSGNSQSPVFKE
                     LMIRWFQFAVFSPILRMHGDRQPHTQKIGNSGGGVRTSGGPNEIWSFGEEVEGILTRF
                     IHIRTRLKDYIRDVYNESATKGLPIMRSLFIEFPDDPKSWEETTHYLFGSDLLIAPVV
                     TEGATHIPVYLPKGCNWIQVFTKKVYPGGQIYKIAVTLNTIPVFCKEGAPLEHHLADI
                     FGN"
     gene            complement(4710..6767)
                     /locus_tag="LNE23_002276"
     CDS             complement(4710..6767)
                     /locus_tag="LNE23_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HBK6932104.1"
                     /translation="MITIEANRIVKKYDDELLIIEPWGQHSLRVRSFLDQHFVERENA
                     LTELHGYDEKLVETYYKNNQATIVNGKIKAVLDHRDRLTFYNEKDEVLLKEFIRLRAV
                     KHDDGSEDVGTVEFTKDFNSTLKLKSREYRANNDGSNFRTKVRFEPHAQEKIYGMGQY
                     QQNYLDLKNTMLELAQRNSQMSVPFYVSSRNYGFLWNHPGIGKVTFAKNMTEWEMYGT
                     DYIDYWITAGDSPKEITEQYTSVTGRVPEMPKKLLGLWQSKLRYRTPEEVLEVVEGYK
                     QRGIQLSTIAIDYFHWPKQGEYRFDPDFWPEPEKLVKTLKEDYNVEPLISIWPTVQSD
                     AYNYEQYLQGGYLIKVNRGVRLTMQIQGSTVFMDTTNPEARDYVWNLIKKNYKDIGID
                     YYWVDVAEPGYAVYDFENYRYHSGTAVESGNLYPNGYLKMVYDGNKDSNESVVTLVRG
                     AWAGAQKYGALAWSGDIDSSFEAFHNQVNSGLNMGMAGIPWWTTDIGGFHGGNPEDPE
                     FRELMVRWFQYSTFSPILRMHGDRLPHSKPLSNSGGGSMPTGAPNEIWSYGSEVENIM
                     TDYVEIRERMKDYLAESMTEAHELGYPIMRTLFYEFPEDKKSWEIDNTYMLGSDILVA
                     PIMNYKERGREVYLPSGEKWRHLFTGKEYAGGATYYVAAPLEQIPVFIKESASELFES
                     LGK"
     gene            complement(6790..8283)
                     /gene="xylB"
                     /locus_tag="LNE23_002277"
     CDS             complement(6790..8283)
                     /gene="xylB"
                     /locus_tag="LNE23_002277"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBK6932105.1"
                     /translation="MGYVLGLDLGTGALKGVLVDETGVIRHIEACDYPLHSPKGGYNE
                     QNPADWLTACGALFEKFSCNVSDFQAELAGISFSGQMHSLVVTDEAGTVLRPAILWND
                     VRNSAQCKRIMDEFGNEIIAITKNRALEGFTLPKILWIQEHEPAIWEKVRKIFLPKDY
                     LRYYLTGHSHMDYSDAAGTLLMDTTKKEWSKPILEKYNIPEVILPPLVESSACVGTLK
                     PEIKATFGFEKEVRIFTGGADNAVSSLGSGLTDSHTALASIGTSGVFLALEEEDHTRY
                     GGKVHLFNHVLPDTYYAMGVTLAAGHSVSWFKNLLAPEQSYDEFLRNIPNVPIGSNGL
                     LFTPYISGERTPHVDSQIRGSFIGLSNHHTRDDLIRAVLEGVTFSLKDSQQLMTSLKG
                     EEFKRIISVGGGAKNKTWLQMQADIFNTEVVSLDTEEGPGLGAAILAAVGLGWFDSFE
                     SCTNQVIRYGTTVQPIPENVEKYNTVYDLYTKVYGATKDLSHALSIL"
     gene            complement(8302..9609)
                     /gene="xylA"
                     /locus_tag="LNE23_002278"
     CDS             complement(8302..9609)
                     /gene="xylA"
                     /locus_tag="LNE23_002278"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBK6932106.1"
                     /translation="MNYFEAVPKIQYEGKQTKNKFAFRYYNPEEEILGKPMKEHLRFA
                     VAYWHTITADGSDPFGAGTYQRNWEGNNPMETAKNRVEAFFEILEKLGAEYFCFHDVD
                     IAPQGDSLEEFFQNIDTITDLIKEKMDQTGIKLLWNTANMFTHPRFVNGAATTNNADV
                     YAIAAAQVKKGLDISKKLNGQNYVFWGGREGYESLLNTNMDFELDNLARFYKMAIAYT
                     KKIGHQPQFLIEPKPKEPSKHQYDFDAATSMAFIMKYGLQDHFKLNLEANHATLAGHT
                     FEHEINVARSYNALGSLDANQGDLLLGWDTDEFPTDIYSTTLAMYEVLENGGIAPGGI
                     NFDAKVRRTSFEMEDLILAHIVGMDTYARGLRAAAKMKEERFFEEIMEEKYASFTQGI
                     GKKIIDDQETFESLSEYALSLSDVTNQSSHIEYIKSLINDYLV"
     gene            10041..11012
                     /locus_tag="LNE23_002279"
     CDS             10041..11012
                     /locus_tag="LNE23_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6932107.1"
                     /translation="MASVSGGRKPIYLSFNNECALVIAIEIGKDYIHSSLSYINGELV
                     NKLSYHSLLVTKDTLVMLLTKIISDLTSKIPQTPYGILGIGMAIHGIVKDDTVLFTPY
                     SDIDSIPLKDLLAKEIPYPIVLINEANAGALGEYTFKTPSENLVNINIQDGIGAGIVE
                     NGKLFIGANGTAGEIGHTTLYPDGILCPCGNHGCLEHYASTRILLKKLLDSDHKPHPD
                     ISVIVKKWHQGDAHTQKILKHNAKLLSIGINNIVLMYDPEVVIINSEIYRGIPELIDE
                     IKKHLTGKNTKNLAIKNTSLKGNSTLYGCVSIVAQSFLKINNLKFTV"
     gene            complement(11103..11414)
                     /locus_tag="LNE23_002280"
     CDS             complement(11103..11414)
                     /locus_tag="LNE23_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932108.1"
                     /translation="MRNKPITVTLFANAYDNELKTNPHSKLKEGTYSVDNDRISFLTK
                     KGEGLVLNLNIQHIDSIATFNQSVIQIKDHNMTEVKNKIYAFSKKSNDKSYWETLAEI
                     I"
BASE COUNT         3237 a         2622 c         2263 g         3722 t
ORIGIN      
        1 gcaaaaagat tgaacgaatt caatcttttt gcttttttat tcatccatat catccatagc
       61 tgatacttgc aacaaatcgt taacataaac aatttgttgt cgggactctt cgatggcttg
      121 gcgaatggtt gtttcactca aatcgttgct accgattgct aaggctaaag tcattaagat
      181 tggcaaatta acgcccgcaa tcaattggat attgtaacgg tctaaaaatt tggcaaaagt
      241 ctggttaacg gaacctccca acaaatccgt gaaaactaat atttgttctt cggcttcgaa
      301 ttggtctaat gccgcttgga cttggttttc aacagaagtg ttttctaggt acgcattaat
      361 agcaatgatt tccaccatat ttggtgcaat atagcctaac gtactcttga acccttccgc
      421 taagcggtag tgtgtagcta caataatttt tttcacctat ttccctcctc gtatgattaa
      481 ggtgcgactc ctaagaaacc gaagtaggcc ccaagtaaag agaacgcgag cagcaagaag
      541 ataaccttta aaaagcccca ttttttcttt tccatcaaga agtaaaccaa tgcggtcaac
      601 aagaccggta acaatgctgg taagatacgg tcaaagactt ccgcctgcaa gcccatggtt
      661 acttcaccga aagtaaattc tattggcgtg taaactttaa caacagtcgc aattaagctt
      721 cccactaccg tcagtcccat gacggaagct gattctgtga tggcgttgag tttatcggcc
      781 atgtcagtga ttaaacgtat ccccgattcg taccccaaat caaacaattt cagacgcatc
      841 cagactaatc cgaggttcaa tataatccaa atgatagcgc ctaatgggtt accttccaga
      901 gccatgtagc cactgataga tcccatgata gttgggtaca atacccatag taaggaatct
      961 ccgataccgg acaaaggacc catcagggat gttttgattg aacggacagc tcccaaggat
     1021 tctgtccctt gttgctcttg tagcgctacg tcagcaccag cgactaaggg tcccatataa
     1081 ggggtgatgt tgaagtactc atattcaacc ctcattgctt ccacgtaatc atcgtcattt
     1141 ggataaattt tacgcaaggt tttcgccatg gaccaggcat agccggttcc ttgcaaggtt
     1201 ccgtagttaa tgttgttact gccgataaaa gaccagcgcc aagctgcatt acgacgatct
     1261 tttttgttta gttcctgtgt aagtcctttt gttctctcat tattcatcga agtcatctcc
     1321 ctcatcgtat gcagcatttt cagttgttac agccactttt tggctcgtta agttattata
     1381 gataaaaact gccagtgcta atccaacaat tgaaacggcc aagattggta attccatata
     1441 tgctgccatt ataaaaccgg ctaacaaagc ccaaatgtag cgttttgttg gcatatagcg
     1501 cattaacatg gctaccccga cgactggcag cataccccca gcgacgttta aaccatttgt
     1561 aatccattct ggaatccatt gggtggctgt ttcaattgct acttgaccaa aggctacgag
     1621 gataaccatc ggaatgaaag cttgcatcgc gacgaagaag acactgatga agttaatcca
     1681 ttgcatctta ctaaatttcc gttcctccaa atattgttta gatttttcgg aaacgaaaga
     1741 gttgattgtc cgcgaaaaga tgtccaagtt aatagccaaa ataccgactg gtaaaccgac
     1801 cgcgatggaa gttcctattt ctgctcctgt acgagcattc aagtaaatcg caacaacggt
     1861 tgccaaacca taatccggta ccgatgatcc acccaatgcg gctactccaa ggctcattaa
     1921 ttgaaacgtt ccagcgataa ccatggcgga ttgcaaatct cccatcacta aaccagctaa
     1981 aagaccaacc gttactggcc agtggttaat aatggtaatt ccccggttat caataagagt
     2041 aagcataggg attaaaataa ttaagatatt ctgtaaaaat tctggcatta taattcctcc
     2101 aatctcaatt tattaaataa atttgtctaa cggttcttct ttgtcggatg gagcaaattg
     2161 gtatgatacc ggaatgccct tatctgcaag ctgttgcaac tgttgcaatt cttctgggga
     2221 agccgcgaaa ttcttagcta actttttgcg gtcgtttcta tccagcataa tcccgatgtt
     2281 cactttcgga atggcgatgc cttgttcact caagtcgagt aacgttttaa cattattaac
     2341 taccaacaac acgttatgac tgtcatattt cccggcctta aaattggtaa tgccagtttc
     2401 tgtgtcaatc aaggacattc ctgtccctgc tggtttgctt agacgcatag ctgcctttct
     2461 tggttcatct tgggcaactt gatcgtccac taccatgata cgttttgctt ttaccgcact
     2521 ggcccattgt gtcacaacaa ttccgtgaac cagtcgttca tcaattcgag ctaatactaa
     2581 tcccattttt catttctcct tttcaatttc caaatatatc tgctagatgg tgttcgagtg
     2641 gtgctccctc tttacaaaat accggaatgg tattcagtgt tacagcaatt ttgtatatct
     2701 gaccaccagg gtaaactttt ttcgtgaaaa cttgaatcca attacaaccc tttggcaagt
     2761 agactggtat gtgtgttgcg ccttctgtca caaccggagc aatcaacaaa tcgctgccga
     2821 ataaataatg agtggtttct tcccaagatt tgggatcatc tggaaattct atgaacagac
     2881 tacgcataat tggtaaacct tttgtagcgc tctcattata tacatcccta atgtaatcct
     2941 tgagacgcgt tctgatatgg ataaagcgcg tcaatatgcc ttcaacttct tccccgaaag
     3001 accatatttc atttggtcca ccgcttgtcc ttaccccccc tcccgaattc cctatttttt
     3061 gggtatgagg ttggcgatca ccgtgcatcc gtagaatcgg actgaaaaca gcgaactgaa
     3121 accagcgaat catgagttct ttgaaaactg gggactgact gtttccacta tggaaacctc
     3181 cgatatcgct cgtccaccaa gtaatgccac ttatgcccat actaagaccg acttgaactt
     3241 gtcttcttag tgaatcgaat gaactttcga tatcccccga ccaagctaat gccccgtatt
     3301 tctgggcgtt aaaccaggca gaacggatta aggtaggttc tgtgttcgat ctctctggat
     3361 ttttctggat tgcctgtaga taatggagag ggtatttatt ggcatatttt tctaagctac
     3421 cctcgtatat aagatattcc ttgtgttggt acacatccat ttctggttcc gcttggtcag
     3481 cccaaaacaa ggaaattccc ttgctccggt aattttcgtc caagacctga cggaggaatg
     3541 tcttactctc tggatggaag aaatctaaca gagcttttcc ggcaaatagc gaatctttat
     3601 ttgtgaccga cctgatcaag agttgattat tccggtagct ctgataataa cgactctcct
     3661 cggttaccgt tggccataga gataccatca gttgggtgcc agtcttcttg atttcttccg
     3721 caaagtcctc gattccttcc caatagcgag aatcaaatgc gaaatcacca tctgaagtcc
     3781 aatgatagta atcaacaacc aaaacggaaa gagaaatgcc tcgttgattg taaccttcat
     3841 aaaccgttct taattcatcc aacgtctgat aacgcagctt gctttgccat aaccccagta
     3901 attccggttc caaccgaggc gtgcgcccag tcatttgcgt ataattttct atgatttgtt
     3961 tcggattttc tccgacggtc accaagtaat caataagatt cgtattgttg ctcttccata
     4021 ttttctggtt gtagccaaat gttacttccc cgattgccgc attattccag atgaaaccat
     4081 actgcttatc ggagagatag aatggtattg acgtctggga gttccgttgc attaactcgt
     4141 acgttccccc gtttttgtta aagttttctt cttgatatcc gcccatcccg tacaattttt
     4201 cattcggatc accttcaaaa cgaagagttg cttggtaggt gttctcgcca gaataggtga
     4261 attctcgagg tgaaatattt aacgatgagg tcggttcgtc cggaatcgga atgtgggtgt
     4321 caatgcctac agtacgtcgg acttgacttt gcttccgaga gtattcctcc aggattattg
     4381 tcccgttgtt gtaaaagacc aacttttctc catcgtatgt ggctgttaaa cggttgttgg
     4441 tcaagattaa ttggtccgac gtctctgtaa cggttgctgt agtcaagacg ttcttctcca
     4501 attcttctat cccttgtttt gggtcgaacc ctttgtggtt gggataacag gtaacgcgca
     4561 ccgtgtcgcg tccccatcct tccactttca ggcattgatt acggtccttc acgacgaagg
     4621 actgttcgga aacaatcact tcttttgatt taaagagcat agcttctttc ctcccaattg
     4681 acttgtcctt tatctcctct ctttatgggc tatttgccta acgattcaaa aagttcagag
     4741 gcggattctt taataaatac cgggatttgt tcaagaggtg ctgcgacata ataagtcgct
     4801 cccccagcat attctttacc ggtgaataag tgtcgccatt tttcaccgct tggtaagtaa
     4861 acctccctac ctctttcttt gtagttcata atcggagcaa ccaggatgtc agaaccaagc
     4921 atgtaggtat tatcgatttc ccaggatttt ttgtcctctg ggaattcgta gaataatgtc
     4981 cgcatgattg ggtatccgag ttcatgtgct tctgtcattg attctgccaa gtagtctttc
     5041 atccgttctc taatttccac gtagtccgtc atgatgtttt caacttcact gccgtaagac
     5101 caaatttcgt ttggtgcacc tgttggcatc gaaccaccac cagaattgga gagcggttta
     5161 ctatgtggca gacggtctcc atgcatgcgc aaaataggag aaaacgtgct gtactggaac
     5221 cagcgaacca tcaattcgcg gaattccgga tcttccggat taccaccatg gaagcccccg
     5281 atgtcagtcg tccaccatgg aataccggcc atccccatgt tcaatccgga gttgacttga
     5341 ttatgaaagg cttcgaaact ggaatcaata tccccagacc aagccaaagc accatacttc
     5401 tgagcacctg cccaagcacc tcgtaccaat gtgacaacgg attcatttga atccttattg
     5461 ccatcgtaaa ccatcttcaa gtatccgttt ggatacaagt tgccactttc caccgccgtt
     5521 ccggaatggt agcgataatt ttcgaaatca taaacagcat aacctggttc agccacatcc
     5581 acccagtagt aatcaatacc gatgtcttta taatttttct tgattaggtt ccagacataa
     5641 tcacgggctt caggattagt cgtatccatg aaaacagtgc ttccttggat ttgcatcgtc
     5701 aagcgaactc cgcgattcac tttaatcaaa tagccacctt ggagatattg ctcatagtta
     5761 taggcatctg attggacagt tggccaaatc gaaatcaatg gttcgacatt gtaatcttcc
     5821 ttgagtgttt tgaccagttt ttctggttct ggccaaaaat cgggatcaaa tcgatactcc
     5881 ccttgcttcg gccagtggaa atagtcaata gcaatagtcg atagctgaat gcctcgctgt
     5941 ttgtatcctt ctaccacttc taagacttct tcaggagtgc gatagcgtag tttactttgc
     6001 cataatccca gcagtttttt cggcatttca ggaacacgtc ccgtaacgct cgtgtattgc
     6061 tccgtaattt ctttaggtga atctccggct gtaatccaat aatcgatata atccgttccg
     6121 tacatttccc attcggtcat gttctttgca aaggttactt ttcctatacc aggatgattc
     6181 cacaagaaac cgtagttccg gcttgaaacg taaaagggaa ccgacatttg tgaattacgt
     6241 tgagccaatt cgagcattgt gttctttaaa tcaagataat tttgttggta ttgccccatc
     6301 ccatatattt tttcctgggc atgcggttca aatcggactt ttgttcgaaa gttcgacccg
     6361 tcgttatttg cacggtattc acgagacttc aactttaaag tggaattaaa atctttggta
     6421 aattcgactg tcccaacatc ctcactgcca tcgtcatgct taacggccct cagacgaatg
     6481 aattctttta agagaacttc atccttttca ttgtaaaacg tcaaacgatc tcgatgatca
     6541 aggacagctt ttattttacc gtttacaatc gtagcttgat tgtttttgta gtatgtctca
     6601 accagctttt catcataccc atgcagttca gttaatgcat tttcccgctc aacaaagtgt
     6661 tgatccaaaa acgaccggac tcgaagtgaa tgctggcccc atggttcaat aattagtaat
     6721 tcatcatcgt atttttttac gattcgattt gcttcaattg taatcatgat ttcctcctat
     6781 tagaattact tacaatatac taagcgcatg agacaaatct tttgtcgcac cgtacacctt
     6841 cgtatataag tcataaacag tattatattt ttctacgttt tccggaattg gttggacagt
     6901 agtaccatac cgaatgacct ggttggtaca agattcgaaa gaatcgaacc aacctagacc
     6961 gactgcagcc aagattgctg ctcccaaacc cggaccttct tccgtatcca ggctcacgac
     7021 ttccgtgtta aaaatatcag cctgcatctg taaccaagtc ttgtttttag cgccgccacc
     7081 cacactaatg atgcgcttga actcttcgcc ttttaaagaa gtcataagtt gttgagaatc
     7141 cttcaaggaa aatgttaccc cttccaaaac agcccgaatc aggtcatccc gggtatggtg
     7201 attactcaat ccgatgaagg acccacggat ttgactgtca acgtgtggtg tgcgttcccc
     7261 agaaatatac ggggtaaata gtagtccatt actgcctatc ggcacattag gaatgtttct
     7321 taagaattca tcgtatgatt gttccggtgc cagtaaattt ttgaaccatg agacgctatg
     7381 tccagcagcc aatgtgacgc ccatcgcgta gtaggtatct ggcaatacat ggttgaatag
     7441 atggactttt ccaccataac gcgtgtgatc ctcttcttcc agggccaaga acacaccgga
     7501 agtaccgata cttgctaaag cggtatggga atccgtcaat ccagatccca atgaggacac
     7561 agcattgtcg gctccaccgg taaaaattcg tacctctttt tcaaagccaa aggtcgcttt
     7621 gatttctggt ttcaatgttc caacacaagc ggatgattcc actaatgggg gtaagataac
     7681 ttcgggaatg ttatattttt ccagtatagg tttggaccac tctttcttag tcgtatccat
     7741 caataaagta ccggcggcat cggaataatc catgtggctg tggcccgtca ggtaatagcg
     7801 taagtaatcc ttgggtaaaa atattttacg taccttttcc cagatagcgg gctcatgttc
     7861 ctgtatccaa aggatttttg gtaaggtgaa tccttccaga gccctgttct tagtgattgc
     7921 aatgatttca ttcccaaatt catccataat gcgcttacat tgggccgaat ttcgaacatc
     7981 attccatagg atagccggtc gtaagacagt tcccgcttca tctgtaacga ccaaggaatg
     8041 catttgtccg gaaaaactaa ttcctgctag ttcagcctgg aaatcagaga cattgcaaga
     8101 aaatttttcg aacaatgcac cacaggctgt gagccaatca gcgggatttt gttcattata
     8161 gccccctttt gggctatgaa gggggtaatc acaagcttca atgtgccgaa ttaccccggt
     8221 ttcatccacc aacactcctt taagggcccc tgttcctaag tctagcccta atacataacc
     8281 catgttttac accccgactt tctatactaa ataatcatta atcagtgatt tgatgtattc
     8341 gatatgactt gattggtttg tcacatctga aagggaaagt gcgtactcac taagcgattc
     8401 aaaggtttct tggtcatcaa taattttttt accgattcct tgggtgaagc ttgcatattt
     8461 ttcttccata atttcttcga agaaacgttc ttctttcatc ttagcagcgg cacgcaaacc
     8521 acgtgcataa gtatccatcc ctacgatatg tgctaaaatc aaatcttcca tttcgaagga
     8581 agtccgacgt actttagcat cgaagttgat accacctggt gcaatccccc cattttccaa
     8641 gacctcatac attgccaaag tcgtggaata aatatctgta gggaattcgt ctgtatccca
     8701 ccccaacaac aaatcacctt ggttagcatc caacgaaccc aatgcgttgt aactgcgggc
     8761 tacattgatt tcgtgttcga acgtatgtcc tgccaacgta gcatggttgg cttcaaggtt
     8821 aagtttaaag tggtcttgta aaccatattt catgatgaac gccattgaag tagctgcatc
     8881 aaaatcatat tggtgttttg atggttcttt tggttttggt tcaatcagaa attggggttg
     8941 atggccaatt tttttcgtat aggcaatcgc cattttgtag aaacgagcca agttatccaa
     9001 ttcaaaatcc atgttggtat tcaataaact ttcatagcct tctcttccac cccaaaaaac
     9061 ataattttga ccatttaatt ttttgctaat atctaaacct ttttttactt gtgctgcagc
     9121 aatagcgtag acatcagcat tattagttgt tgctgctccg ttgacaaaac gaggatgtgt
     9181 aaacatatta gcagtgttcc aaagtaattt aatgcccgtt tgatccattt tttctttaat
     9241 cagatcggta atggtatcga tgttttggaa aaactcttcc aaggaatcac cttgaggagc
     9301 aatatcgaca tcgtggaaac agaaatattc cgctcccaat ttttctaaaa tttcaaagaa
     9361 agcctccaca cggtttttag ccgtttccat tgggttgttg ccttcccaat ttctttggta
     9421 agtacctgca ccgaatggat cggagccgtc tgccgtaatt gtgtgccagt atgctaccgc
     9481 aaaacggaga tgctctttca tcggcttgcc tagtatttct tcttcgggat tatagtagcg
     9541 aaaagcaaat ttatttttag tctgtttgcc ctcgtattgt atttttggta ccgcttcaaa
     9601 ataattcatt gctaattcca cctttcttag ttagttcgaa actcgtacta acctgatatg
     9661 attatattac ctgggtggga acagcttgtc aagcgctttc gttttttaaa aattaccttg
     9721 gttggttagg tgcgatttat attttttccc tgggacttcc tttagaatcc ttgacaagat
     9781 tgaaattata gtaaatgatt taataaaatc tatttcatat ttgaagtatt tcgaaattat
     9841 cttaattaaa ggagcagact aaattgaatg attacacacc gacacattac aatgagtcga
     9901 ccatcctcga attcatcatt caaaacgccg aggtttctcg atcaaaatta gcgaaattaa
     9961 caaacttaac gaagtcgtct atttccgata tcacaaaaac attgattgaa aaatcactcg
    10021 tctacgaatt aaacactgga atggcctccg tatcaggcgg tagaaaacct atttacttga
    10081 gttttaacaa cgaatgcgcc cttgttattg cgatagagat tgggaaggac tatatccata
    10141 gttctttgtc ctacatcaat ggggagttgg tcaataaatt gagttaccat tccctcttag
    10201 taaccaagga tacacttgtc atgttgttga caaaaataat ctccgattta acttcgaaaa
    10261 taccccagac cccttatggt attttaggaa ttgggatggc aatccatggc atcgtgaaag
    10321 atgatacagt cttgtttacg ccttattcag acattgattc cattcctttg aaagacctac
    10381 ttgccaaaga aattccctac ccaatcgtct taattaatga ggcaaacgcg ggtgctcttg
    10441 gggagtatac gtttaaaacc ccttcggaga atttggttaa catcaatatt caagacggga
    10501 ttggggctgg tatagttgag aatggtaagc tgtttatcgg ggcaaacggc acagcagggg
    10561 aaattggaca cactaccctt taccccgatg gcattctatg tccgtgcggg aatcatgggt
    10621 gtttggagca ttatgcttct acccgtattc tgttgaagaa gctgcttgat tcagatcata
    10681 aaccacatcc ggacatctca gtaatcgtta aaaaatggca ccagggggat gcacataccc
    10741 agaaaatact gaagcataat gcgaaattac tcagcatcgg tattaacaat attgtgctaa
    10801 tgtatgaccc agaggttgtc atcatcaaca gtgaaattta ccgaggcatt cccgaattga
    10861 ttgacgagat taagaagcac ttgactggta aaaacacaaa aaacttagct attaagaata
    10921 catctctaaa aggtaattct acactctatg gctgtgtttc catagttgcc cagtcctttc
    10981 tgaaaatcaa taatctgaaa tttacagtgt aaagtgatag aaaaatatcc tccttaaaac
    11041 ttcctatttt tcgggaggat atgattttat tagggtgtaa gtacttagca gcattttttt
    11101 cactaaatga tttcagctaa cgtttcccaa taacttttat catttgattt tttagaaaaa
    11161 gcataaattt tgttttttac ttccgtcata ttatgatctt ttatttgaat aacgctctga
    11221 ttgaaagtag ctatactgtc aatatgctga atatttaaat ttaacactaa tccttcccct
    11281 ttttttgtta aaaatgaaat ccgatcatta tctacgctat aagttccttc ttttaatttt
    11341 gaatgaggat ttgtttttag ctcgttatca taagcgttag caaataacgt aacggttata
    11401 ggtttatttc tcattttttc gtctctcctt tttggtctag agttttacaa cttttttctt
    11461 ggcgtttgtc aagaagccta tcttttgatc ttatttttta tttttttaat tatcttctgt
    11521 tctgttcata cagttgactc ctttcttatt tagtcctttt ttcaagtatg tccgtctgtc
    11581 gcctgttttg cccttatcat gagggattta gggaagtaaa gggcgagtgt agcgagtgca
    11641 ttttaccctt tacttcccta aagaacttat gatataaacc ggttggcgag agactgactt
    11701 gaaaatagga cgtgaataga agtaataggt tatgaacaaa cgggtaattt tttcaataaa
    11761 aaaacgaaca aaagcagcaa tctttttcga ttgctgcttt tgttcgtttc atctgtttta
    11821 attgcattta tttttgggta tttt
//
LOCUS       DAFCNS010000079        11447 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFCNS010000079 DAFCNS010000000
VERSION     DAFCNS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.081"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..54)
                     /locus_tag="LNE23_002281"
     CDS             complement(<1..54)
                     /locus_tag="LNE23_002281"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBK6932109.1"
                     /translation="MLVFQMRNVDKTSTVLKQ"
     gene            complement(103..342)
                     /locus_tag="LNE23_002282"
     CDS             complement(103..342)
                     /locus_tag="LNE23_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBK6932110.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            complement(476..1693)
                     /locus_tag="LNE23_002283"
                     /pseudo
     CDS             complement(476..1693)
                     /locus_tag="LNE23_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            2184..3122
                     /locus_tag="LNE23_002284"
     CDS             2184..3122
                     /locus_tag="LNE23_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6932111.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            3252..3899
                     /locus_tag="LNE23_002285"
     CDS             3252..3899
                     /locus_tag="LNE23_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HBK6932112.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(4592..5530)
                     /locus_tag="LNE23_002286"
                     /pseudo
     CDS             complement(4592..5530)
                     /locus_tag="LNE23_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(5530..6147)
                     /locus_tag="LNE23_002287"
     CDS             complement(5530..6147)
                     /locus_tag="LNE23_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6932113.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(6161..6331)
                     /locus_tag="LNE23_002288"
     CDS             complement(6161..6331)
                     /locus_tag="LNE23_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932114.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(6679..8169)
                     /locus_tag="LNE23_002289"
     CDS             complement(6679..8169)
                     /locus_tag="LNE23_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBK6932115.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(8212..8511)
                     /locus_tag="LNE23_002290"
     CDS             complement(8212..8511)
                     /locus_tag="LNE23_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932116.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8877..9161)
                     /locus_tag="LNE23_002291"
     CDS             complement(8877..9161)
                     /locus_tag="LNE23_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6932117.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(9680..10165)
                     /gene="vanZ"
                     /locus_tag="LNE23_002292"
     CDS             complement(9680..10165)
                     /gene="vanZ"
                     /locus_tag="LNE23_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBK6932118.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(10318..11229)
                     /locus_tag="LNE23_002293"
     CDS             complement(10318..11229)
                     /locus_tag="LNE23_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBK6932119.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3508 a         2005 c         1743 g         4191 t
ORIGIN      
        1 ctgtttcaaa acagtagatg ttttatctac attacgcatt tggaatacca acatgacgaa
       61 tccctccttc ttaattacaa atttttagca tctaatttaa cttcaattcc tattatacaa
      121 aattttaaga taatgcacta tcaacacact cttaagtttg cttctaagtc ttatttccat
      181 aacttctttt acgtttccgc cattctttgc tgtttcgatt tttatgatat ggtgcaagtc
      241 agcacgaaca cgaaccgtct tatctcccat tatatctttt tttgcactga ttggtgtatc
      301 atttcgtttt tctttttgtg cgcctaaatt tcccacaatc actcacttct ttctatttct
      361 tcttattctt attttatcat caacaatcac aaatcacttg tgatttgtga taagtgattt
      421 gtgataagtg atttgtgatt aatatataaa agccctcttt aaagggcttt tatgtttatt
      481 ttgagaaaga tataaaatca atatatccct tttccccaat ttttacaacg gcattgtagg
      541 gctttttctc tttgtttttg attcctttta ccagggtttc ttttccctcc agtaattctt
      601 ttacattcgt tttggttagt tttttctttc taaaatgttc agctaaagta aacttacatt
      661 caggataatt tgaacaacca taaaacgatt tttttaatac aatattgttg ccacacttag
      721 gacattttcc tacaatactt ttttctgctt ctttttcttt ctgttcctgg taatcagaaa
      781 aatttagttt ttctatatcg ttaggtacag cttccagtaa atgaacaatg aattttttga
      841 tattcgtaat aaagttctct tgattgcctt ctcttttacc gatttttttt aaatacgttt
      901 cccatttagc cgtcatttca gcactcgtta aaaggtgctg actttcaact gcctggcaca
      961 ataattttcc tttttcagtt acaacaagct tattcttaat cacttggata tattcttttt
     1021 gttttaacgc ttcaataata cttgctcttg tcgcttctgt tccaatcccc tcaacttctt
     1081 gtaaaatctt gattgcttct tcatcatcaa ccgttttatt cgccgttttc atagcagtta
     1141 ataatgtacc ctctgtaaaa gcttttggcg gttgcgtttc tttttcaaca ctcttaacac
     1201 caacttcggc acgttcgcca atgataacga gtggtaacgt ttgaacgtcc tctttttctt
     1261 ctttggtttg ttgtttgaaa agaattttcc acccttcttg ctttggtgtc tttccgatcg
     1321 attgaaacag caaatcagct actttcgttt gtatcttggt ttcttcatac aagtaatcag
     1381 gtaaaaacat ggcaacggtc gttttaacga ctaaagcata aatttttcgt tgtaaatcgt
     1441 ccattttcgc taatgcagat tcggtaggta cttgttttgt tgggataatg gcgtggtgtt
     1501 cctgtacctt actaccgtcc acataacgct ttctaggctc tgtttgaacc atttcaagat
     1561 caagtcctaa aaaaccgcta tatttgccaa aattcgcttt taaataagca aattcgttct
     1621 ctgtaataaa tggtgtatct gttcttggat aactcaataa ttttgcttca tacagtcctt
     1681 gcatagcttt taaacgtaaa attttgcgtt ctaagacgtt ttaaatatat ctgcgtatat
     1741 ttttatgtgt tttatctttt cgtcggcaaa tgcgttaaaa tttgcgaaat aaaatagctc
     1801 aagggcgaac gcagtgagtg catttaccct tgatctattt ttttgagtaa attagctttt
     1861 gaaaccgacg gaaaggaaac acaaaacttt tcagaggcaa ggggttttta aggggcgctt
     1921 ttagcccctt gaatttattt gctttaagat atatttagtt ttaaaatagt tttaacgaaa
     1981 aaaagacata aaaaaagacg agcaacgcta ccaacgttct cgcccggaat aacaaaccac
     2041 acttgttatt cattcgatga ttagtatatc aatttttgat tttgaataca aaacaaaatg
     2101 gacgaatgga tacaggcgaa atttattttg cgattccact cgtccttttt tcgttccaaa
     2161 aattatttta ggagcgataa aaaatgaaga aaattgatga aaaattagct gcagaaatct
     2221 cactaaaaaa accggccgtc tctaatagcc ggttaagtaa tagccggtta agtggtcaaa
     2281 ctttgggaaa atctcaaccc gcattaagtt ttgatgccat gacaatcgtt ggaaatttga
     2341 acaaaactaa tgctaaaaag ctatctgact ttatgagtgt agagccacaa atacgacttt
     2401 gggatatact tcaaacaaag tttaaagcta aggcacttca agaaaaagtt tatatcgaat
     2461 atgacaaagt aaaagcagat acttgggata gacgtaatat gcgtgttgaa tttaatccca
     2521 ataaactcac acatgaagaa atgatttggt taaaacaaaa tattatcgac tacatggaag
     2581 atgacggttt tacaagatta gacttagctt ttgattttga agatgatttg agcgattact
     2641 atgcaatgac tgataaagca gttaagaaaa ctgtttttta tggtcgtaat ggcaagccag
     2701 aaacaaaata ttttggtgtc cgtgatagtg atagatttat tagaatttat aataaaaaac
     2761 aagaacgtaa agataacgca gatgttgaag ttgtgtctga acatttatgg cgtgtagaag
     2821 ttgaattaaa aagagatatg gttgattact ggaatgattg ttttaatgat ttacacatct
     2881 tgaaacctgc gtgggctact ttagaaaaaa ttaatgagca agctatggtt tatactttgt
     2941 tgcatgaaga aagtatgtgg ggaaagctaa gtaagaatac taagactaaa tttaaaaaat
     3001 tgattagaga aatatctcca attgatttaa cggaattaat gaaatcgact ttaaaagcga
     3061 acgaaaaaca attgcaaaag cagattgatt tttggcaacg tgaatttagg ttttggaagt
     3121 aaaataagtt ttatttgata aaaattgcta attcagtata attaatattt acgaggtgac
     3181 ataacgtatg aaaaaatcag aggattattc ctcctaaata taaaaattta aaatttagga
     3241 ggaagttata tatgactttt aatattattg aattagaaaa ttgggataga aaagaatatt
     3301 ttgaacacta ttttaatcag caaactactt atagcattac taaagaaatt gatattactt
     3361 tgtttaaaga tatgataaaa aagaaaggat atgaaattta tccctcttta atttatgcaa
     3421 ttatggaagt tgtaaataaa aataaagtgt ttagaacagg aattaatagt gagaataaat
     3481 taggttattg ggataagtta aatcctttgt atacagtttt taataagcaa actgaaaaat
     3541 ttactaacat ttggactgaa tctgataaaa acttcatttc tttttataat aattataaaa
     3601 atgacttgct tgaatataaa gataaagaag aaatgtttcc taaaaaaccg atacctgaaa
     3661 acaccatacc gatttcaatg attccttgga ttgattttag ttcatttaat ttaaatattg
     3721 gtaacaatag cagcttttta ttgcctatta ttacgatagg taaattttat agtgagaata
     3781 ataaaattta tataccagtt gctctgcaac ttcatcattc tgtatgtgat ggttaccatg
     3841 cttcactatt tatgaatgaa tttcaagata taattcatag ggtagatgat tggatttagt
     3901 ttttagattt tgaaagtgaa tttaatttta tacacgtaag tgatcataaa atttatgaac
     3961 gtataacaac cacatttttt ggttgcttgt ggttttgatt ttgaatttgg ttttgaactt
     4021 atggactgat ttattcagtc cattttttgt gcttgcacaa aaactagcct cgcagagcac
     4081 acgcattaat gacttatgaa acgtagtaaa taagtctagt gtgttatact ttacttggaa
     4141 gatgcaccga ataaaaaata ttgaagaaca actagcaaaa gattttaaag agttatttta
     4201 ttttaagtct ttataacatg agtgaagcga atttttaaat ttcgatagaa atttttacat
     4261 caaaaagccc cctgtcaaaa ttgacgaagg gggttttttg gcgcacgctt ttcgttagaa
     4321 atatacaaga ttgaaaatcg tgtataagtg cgccctttgt tttgaactta gcacgttaca
     4381 tcaatttttt aaaatgatgt ataagtgcgc ccttttaaat tttgagtgat tatatttttt
     4441 gagttagaaa aagggattgg gaaaatttcc caaaataatt taaaaaataa gcaaaaattt
     4501 tcgatagaga atgtgctatt ttttgtcaaa ggtgtatacc ttgactgtgc ttgctgttac
     4561 attaagttta tttttaagtt attaaaaaag aaatagcttt taaagtttgg ctcgctgtcg
     4621 ctttataaag ctgattgact tttgattgca aactacttaa agaaaacaaa ctcggactat
     4681 tcgttttctt ctctttggtt tgaacatcag caattatccc ctcttgattg cctattttag
     4741 cttgtttaga agaaacaaaa gctaaaagct cctcttgggt tttaaaacgc tgtgtggggc
     4801 ttagaacgcc cttaaacgac ccttggttta cttttatact agcttccacc tcgaaaaaag
     4861 gttctttttt aaaattctct atggcttcct ggcgctgaaa aataaggtat aaggtgggcg
     4921 tttgaacacg tcctagtgaa aatgtacctt gtacgccctt ctgttgtaaa tttaacgtat
     4981 acaaagggct tgcgttcatg ccgatcaacc aatcggcaat ttggcgtgtt tgcgcttctt
     5041 gataaaaggg atagtaattc attccaggtt gcaaattttg aaaaccgctt cggattacat
     5101 ctttttctaa gctattgatc catagtcttt taaatgtttt atcttttgaa aaggcatttg
     5161 ctttatggat aatcgaccag gcgatatttt caccttctct gtcgctatct gttgcaacaa
     5221 taattgtatt tgcctttttg agaagttctg caacaatttt aaactgcttt cccttatctt
     5281 ttgcaacttc aaaatcgtat cgatcaggaa aaatcggcaa agattcaagt ttccaatttt
     5341 gccacttttc gtcataatga cctggttctg ctaattccac taaatgccca aaaccaaagg
     5401 tgataaacgt ttcatctgta aatagtgggt ctttgatctc aaaataaccg tcttttttgg
     5461 tgctttgttt taaagcactt gcgtaggcta atgcctggct tggtttttca gctaaaataa
     5521 ccgtactcat taactatccc tcttttcatt gttttttctt tgatcgactg tcacgttata
     5581 tcttgctcga taccttctaa acgttcggcg attgattcca gtttgttctt caacttcttt
     5641 atcggataaa ccattcaaaa acaaatcgaa agcatgcttt aaacgtggat cattttcttt
     5701 aaatttatgc tgacgacctt tgaatttgcc ttttttctta gcaatttcga ttccttgtgc
     5761 ctgacgttcc ttaatttttt ttcgttctga ttctgcttga tacttgtaca attcaatgac
     5821 aaggctatta atcaaacgcc ttaaattttc atcttcaata ccattcattg agggtaaatt
     5881 taagacttcc agggttgccc ccttaatttg aatttgattc atcaattctg ttaattcttt
     5941 attatttcgt cctaatcgat ctaattcagt aacaataaca atatcccctt cacgaatata
     6001 gttaagcata gcttgtaatt gtgggcgttc gaccgattga ccgcttaatt tgtctgaaaa
     6061 gaccttagaa acgccctgta acgcttgtaa ttgccgatct aagttctgtt ctttgctact
     6121 gacacgtgca taaccaattt tagccatttt caaccaacct ctaaaattct ctcggttgca
     6181 ataaccaatc agcaatatct actttttcaa tttcaaattg cttatcagaa attgtctttt
     6241 cgtaagcgat aaaatcttgc gcatattgtt gctcattaaa aatagccacc acttcgtcat
     6301 tttctaaaac tcgataaata aattttttca ttttactcct cctattatgc ccaacttaaa
     6361 tgacctattc accaagtcaa ttatactgct aaaatcatat taggacaaat aggtatactc
     6421 taatgaccta taaaagatag aaaattaaaa aatcaagtgt tcgcttcgct ctcactgccc
     6481 ctcgacgttt tagtagcctt tccctcactt cgttcagtcc aagccaacta aaagttttcg
     6541 ggctactctc tccttctccc cctaataatt aattaaaatc ttactctgta tatttctgct
     6601 aatcattcgc taaacagcaa agaaaaaaca aacacgtatc atagatataa atgtaatggc
     6661 atagtgcggg ttttattttc agcctgtatc atagctaaac aaatcgagtt gtgtgtccgt
     6721 tttagggcgt tctgctagct tgtttaaagt ctcttgaatg aatgtatgct ctaagtcaaa
     6781 agaatttgtc agcgccttta tatagctttc tttttcttct ttttttactt taatgatcga
     6841 tagcaacaat gatttaacac tagcaagttg aatgccacca tttcttcctg gtttaatctt
     6901 aaagaaaatt tcctgattcg ccttcagtac cttcagcaat ttatctaatg tccgttcagg
     6961 aatgcctagc acttctctaa tctctttttt ggtcgtcact aaataaggct tgtatacatc
     7021 gcttttttcg ctaatataag ccattaaatc ttctttccat tctgacaaat gaacacgttg
     7081 acgttcgctt ctttttttct tgaatttaaa ccacccttga cggacaaata aatctttact
     7141 ggttaaatca cttgataccc aagctttgca aagaatggta atgtattccc tattagcccc
     7201 ttgatagttt tctgaatagg cacttctaac aattttgatt acttcttttt cttctaaggg
     7261 ttgatctaat cgattattaa actcaaacat attatattcg cacgtttcga ttgaatagcc
     7321 tgaactaaag taggctaaag agagggtaaa catgacgtta ttacgcccta ttaaaccctt
     7381 ttctcctgaa aatttcgttt cgtgcaataa gagattaaac cagggttcat ctacttgttt
     7441 tttgccttct gtaccgctta aaaccgttag acttgaacga gtaaagccct tattatctgt
     7501 ttgtttgaaa gaccaatctt gccattcttt gaaagaataa cggtaattag gatcaaaaaa
     7561 ttctacattg tccgttcttg gtatgcgagc aataccaaaa tgattacacg ttagatcaac
     7621 tggcaaagac tttccaaaat attctcggat attttgcgaa attattttgg ctgctttgac
     7681 agatttaaat tctgattttg aagtcacata gactggcgtt tctaaaacaa aatatgcttg
     7741 ataaccttta tcagatttga taatcatagt aggcataaaa cctaaatcaa tagcggttgt
     7801 taaaatatcg cttgctgaaa tagtttcttt tgccgtgtga atatcaaaat caataaagaa
     7861 ggtattgatt tgtcttaaat tgttttcaga atgtcctttc gtgtatgaac ggttttcgtc
     7921 tgcatacgtt ccataacgat aaacgttggg tgtccaatgt gtaaatgtat cttgattttc
     7981 ttgaatcgct tcctcggaag tcagaacaac accacgaccg ccaatcatgc ttgattttga
     8041 gcgatacgca aaaatagccc ctttgctttt acctggcttg gtagtgattg agcgaatttt
     8101 actattttta aatttgtact ttaacaagcc gtcatgaagc acagtttcta caacaaaagg
     8161 gatattcatt cagctgttct cctttcctat aaatcctata aaataggttg tttaattaac
     8221 ttggtttgct ttttcattca actgtttcaa tattgcatgt tttgaaaaag atttttttcc
     8281 tttataagtc aatttttttc cactaatcga ataaattatt ttgttatttt ctattaactt
     8341 atatatataa tcttccccct ccgaagaaaa atacttatct gattttgttt ctaagtagat
     8401 atttctcttt tctaactctt tcttaaacgt ttctagtgta tagatatttg ctaattttct
     8461 tatctccaat aaactatttt ttatataagt tttacattca tcatgattca tacaaactcc
     8521 accttctata aatgaataca aaaaaagcaa tcaaacgatt tccgattgat tgcttaacaa
     8581 ttcttaaatt cagtagctta gatacttgaa aactctctga tttccctata taatgatagt
     8641 acggttatat accgtcttca aacaaagtta attaaataac ttcttacgag ggaagagttc
     8701 atctgactaa ctgataagcg ttggtttggc aatcttatcg ggctatgcat ttataaaatg
     8761 tcgtcaaaca ttttataaat gtgtcatggc tcttttttcg tttctattca gttcgttgtt
     8821 tcgttatatc tagtataccg cttttaaaaa aaataagcaa cgatttcgtg cattattcac
     8881 acgaagtcat tgcttttttc ttcttccatt tctaaatcca atgttacttg ttctgattct
     8941 gtttctggtt ctggttctgt tggctcattt gggattaaat ccactactag cgttgagtta
     9001 gttaactttg caatttgttc tagtgttttt atggttggat ctgattttcc tgattctatt
     9061 cgtgaataat ttgatctact catttctaat tcttgggaga atttttcttt tgatttggta
     9121 cctctcatct tttttaagct ttctctaaat gctagttcca agtttctccc ccctcttttt
     9181 gtgtaataaa attcacgctt tatttaaatt atagctttta atatcttaat aaacaagtga
     9241 ttaatcactt gtgatttgtg attattccaa aagtaggtaa tttcaccaac ctttttcaaa
     9301 agtttttatc gacgattatt ggactggatc gtgtttccta aagcgattct aagcgcatgt
     9361 ttaattttaa agtgggtaat tctctttaaa acgtcctaca aagattcaaa aaaagcatgg
     9421 ctgctagtgg gggtaccgtc aggaaaatgc ggatttacaa cgttaaggaa atttattaaa
     9481 gagaccaatt cctcagcact aaaattgata attgggcttt ctttgttact atagtgttag
     9541 gtaagctttt tactttatca ccggttttcc cctcacttca cacctacggg cgagtttccc
     9601 cacatcccgt tttccatcta atccaattca ttcaatatct ttatgtgtat gaaagatcag
     9661 attgcttgat ttagacatct tacacgtaat ttattcttaa atgggtacgg taaacgagca
     9721 ataatacgag caaaagtata cctacaaaaa taataactct gtctaatttt ttttgattca
     9781 tatgcttatt gcttaaacca tataatttca gtccaagaaa gcctccaaca gtatttgtaa
     9841 ttacatctgt tatgtctgtc gctccaatag cgaagataaa ttgaattatt tcaaaagtaa
     9901 gacttaaaac cagtacaaaa gcaaacttag gtaaaaatcc gatttcttta aaattgacat
     9961 tcaaaagcaa gccaaatgga ataaagatta taacattatc tatcatctct ctgaaattcc
    10021 cagtagcagt aaatggagtc aagttaagac ttctttgatg ataattaaat actgataaaa
    10081 tattgtattg taatttgaat aacactaacc agattagtgt cactaaatat aaagctagca
    10141 atcctctaga taatattttt cccaatataa tttcctctaa ttcttttgtt taagttgtat
    10201 cattagtata gcaatgtcct agttaacgtg ggttttaaat taaaaagcta acgttacccc
    10261 aaaagtgcta gtacaataat gctgttcatt aatttcgttg gtttcattcc gccatcctta
    10321 cctccttgaa ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt
    10381 gtttcctgat atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac
    10441 aggataataa aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc
    10501 ttttaggtaa tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg
    10561 tggtaaacca acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt
    10621 gtcctctgga taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc
    10681 ttcaggggct cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt
    10741 atgctcacta taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact
    10801 ttgctcatca aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac
    10861 agcatcattg accatctctg aaaatttttg tgctatttct tttgacatat aaatattact
    10921 atcaagcaac ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga
    10981 cttcacactt tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata
    11041 aacctgttct tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa
    11101 atgttctttg ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc
    11161 ttcattaacg tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa
    11221 cttcttcatt ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa
    11281 tcttcccctt aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca
    11341 gtcaattatt tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag
    11401 tgatacccat gctgaattta acgaggctga actgcctgtt gacaatg
//
LOCUS       DAFCNS010000080        11150 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFCNS010000080 DAFCNS010000000
VERSION     DAFCNS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.082"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="LNE23_002294"
     CDS             6..305
                     /locus_tag="LNE23_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6932120.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LNE23_002295"
     CDS             378..1739
                     /locus_tag="LNE23_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK6932121.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LNE23_002296"
     CDS             1761..2021
                     /locus_tag="LNE23_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932122.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LNE23_002297"
     CDS             2035..2787
                     /locus_tag="LNE23_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBK6932123.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LNE23_002298"
     CDS             complement(2881..3486)
                     /locus_tag="LNE23_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6932124.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="LNE23_002299"
     CDS             complement(3542..4516)
                     /locus_tag="LNE23_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBK6932125.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="LNE23_002300"
     CDS             complement(4894..5613)
                     /locus_tag="LNE23_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6932126.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="LNE23_002301"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="LNE23_002301"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK6932127.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="LNE23_002302"
     CDS             7164..8561
                     /locus_tag="LNE23_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6932128.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="LNE23_002303"
     CDS             8642..9082
                     /locus_tag="LNE23_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6932129.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="LNE23_002304"
     CDS             9229..9555
                     /locus_tag="LNE23_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6932130.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="LNE23_002305"
     CDS             9556..10305
                     /locus_tag="LNE23_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932131.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="LNE23_002306"
     CDS             complement(10331..10741)
                     /locus_tag="LNE23_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK6932132.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3538 a         1948 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaattaa
//
LOCUS       DAFCNS010000081        11113 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFCNS010000081 DAFCNS010000000
VERSION     DAFCNS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.083"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            136..309
                     /locus_tag="LNE23_002307"
     CDS             136..309
                     /locus_tag="LNE23_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932133.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="LNE23_002308"
     CDS             949..1422
                     /locus_tag="LNE23_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBK6932134.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="LNE23_002309"
     CDS             1431..1658
                     /locus_tag="LNE23_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6932135.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="LNE23_002310"
     CDS             2294..2665
                     /locus_tag="LNE23_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932136.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="LNE23_002311"
     CDS             complement(2921..3163)
                     /locus_tag="LNE23_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6932137.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="LNE23_002312"
     CDS             complement(3195..4097)
                     /locus_tag="LNE23_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6932138.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="LNE23_002313"
     CDS             complement(4110..4298)
                     /locus_tag="LNE23_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBK6932139.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="LNE23_002314"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="LNE23_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBK6932140.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="LNE23_002315"
     CDS             complement(4913..5806)
                     /locus_tag="LNE23_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6932141.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="LNE23_002316"
     CDS             complement(5884..6807)
                     /locus_tag="LNE23_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK6932142.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="LNE23_002317"
     CDS             complement(6856..7206)
                     /locus_tag="LNE23_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK6932143.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="LNE23_002318"
     CDS             complement(7239..8141)
                     /locus_tag="LNE23_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBK6932144.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="LNE23_002319"
     CDS             complement(8134..8991)
                     /locus_tag="LNE23_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBK6932145.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="LNE23_002320"
     CDS             9325..10134
                     /locus_tag="LNE23_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6932146.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="LNE23_002321"
     CDS             10174..10671
                     /locus_tag="LNE23_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6932147.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         3248 a         2082 c         1869 g         3914 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtg gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa cca
//
LOCUS       DAFCNS010000082        10918 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFCNS010000082 DAFCNS010000000
VERSION     DAFCNS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.084"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..561
                     /locus_tag="LNE23_002322"
     CDS             <1..561
                     /locus_tag="LNE23_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBK6932148.1"
                     /translation="PVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKI
                     DYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNS
                     KYTKLLIKNFLISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKE
                     DYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            596..1363
                     /locus_tag="LNE23_002323"
     CDS             596..1363
                     /locus_tag="LNE23_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6932149.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1555..1953
                     /locus_tag="LNE23_002324"
     CDS             1555..1953
                     /locus_tag="LNE23_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBK6932150.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2260..2634)
                     /locus_tag="LNE23_002325"
     CDS             complement(2260..2634)
                     /locus_tag="LNE23_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932151.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2656..3033)
                     /locus_tag="LNE23_002326"
     CDS             complement(2656..3033)
                     /locus_tag="LNE23_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932152.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(3023..3418)
                     /locus_tag="LNE23_002327"
     CDS             complement(3023..3418)
                     /locus_tag="LNE23_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBK6932153.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(3485..3670)
                     /locus_tag="LNE23_002328"
     CDS             complement(3485..3670)
                     /locus_tag="LNE23_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBK6932154.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(3752..4006)
                     /locus_tag="LNE23_002329"
     CDS             complement(3752..4006)
                     /locus_tag="LNE23_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932155.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(4028..4273)
                     /locus_tag="LNE23_002330"
     CDS             complement(4028..4273)
                     /locus_tag="LNE23_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932156.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(4280..4564)
                     /locus_tag="LNE23_002331"
     CDS             complement(4280..4564)
                     /locus_tag="LNE23_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932157.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(4667..4849)
                     /locus_tag="LNE23_002332"
     CDS             complement(4667..4849)
                     /locus_tag="LNE23_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBK6932158.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(5055..5594)
                     /locus_tag="LNE23_002333"
     CDS             complement(5055..5594)
                     /locus_tag="LNE23_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932159.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKIYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(5707..5973)
                     /locus_tag="LNE23_002334"
     CDS             complement(5707..5973)
                     /locus_tag="LNE23_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6932160.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(5975..6196)
                     /locus_tag="LNE23_002335"
     CDS             complement(5975..6196)
                     /locus_tag="LNE23_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBK6932161.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            6422..7240
                     /locus_tag="LNE23_002336"
     CDS             6422..7240
                     /locus_tag="LNE23_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6932162.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            7244..7540
                     /locus_tag="LNE23_002337"
     CDS             7244..7540
                     /locus_tag="LNE23_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932163.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(7722..8075)
                     /locus_tag="LNE23_002338"
     CDS             complement(7722..8075)
                     /locus_tag="LNE23_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932164.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(8095..9150)
                     /locus_tag="LNE23_002339"
     CDS             complement(8095..9150)
                     /locus_tag="LNE23_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBK6932165.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYKNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            9366..9764
                     /locus_tag="LNE23_002340"
     CDS             9366..9764
                     /locus_tag="LNE23_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932166.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            9767..9940
                     /locus_tag="LNE23_002341"
     CDS             9767..9940
                     /locus_tag="LNE23_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932167.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         3635 a         1679 c         1859 g         3745 t
ORIGIN      
        1 cctgttttct ccattgcaga tttacgaaag aaatttagta ttgagaaaaa atataaagct
       61 tttcgagact ttgaaagacg tgtaattaaa gaaccgattg cccaactgaa taaatacact
      121 gattttaaaa ttgactatga gcttatcaag aagggtcata cttatacaaa cataaagttc
      181 ttgcttacac gtaccatgtc agttccggta ccttataagc aagaacaaag agaccctgct
      241 tatttaaaag gcgcagaaga taaaacagag aaaatgaatc aatcgtactt gaaagctatg
      301 aatagcaaat acactaaatt actaatcaaa aactttttga tcagttctgc cgacttatgc
      361 gatcttaaac ttatgagccg acttcagaat aaagtttatc cgctttatga tcgtttatcc
      421 aaagatagag gatttgaatc agttgtcaat cacctatctt acgttgctac tcataaggag
      481 gattacacaa taaaaaatac tgttaaatat ctcgaaaaat caattacgag ttatttgcct
      541 tatgtgaacc tttatgatta acaactgaaa aaatgcgtga aggaaggaaa aacttttgaa
      601 aaaagatgaa ctgacaaagt acaatgagga gttcttagca acaattgaca tgaaaatgga
      661 tttaaagtcg tatgaacttt atcaattgct gttcagttat atctcaccca caaagctaaa
      721 gaaaacaggc gtagtagatt tacctgttaa agcgattatt aattacatgg atgttgatca
      781 aaatcttgaa acggaactaa aaaccatagt taacactttg caaagacaag cagtttatga
      841 aatttcgaat ctagacaaaa aatataagac gtatactact gtatctgcta ttgatcggat
      901 tcattgggat ttagaagagc atagtgtatc tttcagtttt tcagaagatg taattccctt
      961 aattgtctca atcaaaaaga agtatccgtt ctttgttttt gaagacttag catcttttca
     1021 aaaaaaatat agcatcgtgt tatacaaata tttggcgatg tattataaaa aatatcttat
     1081 cgccaaaaat aaaggagatt ttgagaaagc tgaaatatat aagaatcccc tgattgaaat
     1141 agttaaaatt cgtgagatta cgaaaacaga aaatgagtat aaaacaatat cgaattttga
     1201 tagacgtgtt ttaagggaat cagttgagga aattaatgaa aaatcaatgc tgaatattga
     1261 ttttgctaaa gttagaaaag gcagaaatta caattttgct gtccagttta atatttctag
     1321 tgagtttgat gaaaaaactt atagagaaaa aaaattaggt taatgcaaaa tatatcttac
     1381 tcgtttgaag atgcttcaga cgtatgctaa gatatatagt gccttgggag caaggtatta
     1441 atctccttta gaggttaatt agcttgtgat cgcctatcaa tttttttggt aggcgattct
     1501 tttttttgaa acaaaaaagc aaaaattatt ttttaattag agaggtgcaa tgcgatgaaa
     1561 tacttttttt gtgcagtatt cgaatggaaa ggaaatgaat atgcagttaa attcccagat
     1621 tttccagact tatatacaca tggtgaaact ttagaagatg cttttgagat ggcgcaagaa
     1681 gtattggaag gatacttggt gcttttagag ttgcaagaaa aagaaattcc atcgcctacg
     1741 cctgttcaag agttaaatgc aagaatcaag cgatctgaga atgttaattt atttgtcgta
     1801 aaagctaata cgaaagttgc taaagctcga aacaagcaaa attatgatcg aaaatcagtg
     1861 actgtggaac actatttaaa tgttttagcg gctgaagaga atattaatct gtcgaaagct
     1921 ttgaatgatt tgttgagaga aaagttgatt tgatgtctct cagatgatca tattaagcaa
     1981 aaacagcttg ttttaggacg tatggattta agaaagacaa attatcaaag aatatattaa
     2041 aagctctcag aagccttctg agagctttta aattgctatg tattgtactg catctgctca
     2101 aattgatttt cttttcatgc ttaccatgta tctaattttc catcaagtaa tttttagata
     2161 tgtattgagt aataaatagc aaatttgctt gatataagct cgaattaggt gctttgtaat
     2221 gtgttaggat aaaattatac aagaaaatgt caaaagctct cagaacgctt ctgagagctt
     2281 tttatcggta ggcttgttta acgtgatctt gaaggatacc cttttgatgt ttctctcgat
     2341 ccctgcatcg ctaattacta agtgttcttc aagtgtgatg taggtatata aattaagcat
     2401 caaaatggtg tagataagca aaaaactccc taaaactaag tgttgattat aaaaatctga
     2461 cttataaaaa agaactgaga atgttagtgt tgattcccaa aaaagaaaca aaattttgta
     2521 tttccaaaga tagtggatca agcgctgtgc tttacccatc tcaaataatt tataataacc
     2581 tgctaattga tcagcaagcg tatcgttaac ggtttctgtt tgtgtttgtt tcatctttct
     2641 cttctcctgt ttcaattaat ctaaaatttc agattgagca agcaactcat tgtggtagtc
     2701 ggtttgaaat tcaaataaca attttgcctt cttaacgagg tttaaagcgt tcgttagtga
     2761 ttcacttaat gcttcaacat ctttaactga ttttgcatgt ttcatttttt cagtgtgcgt
     2821 atagcagaat tcaggtattg agttaggggt tgcctgttca ataaacatct caattcgcaa
     2881 gttattatcc tcaaaaataa ttagatcatt ggcaaagtgt ttggtactaa cgctaatgcc
     2941 agttgtatct tttaatagat tattgattaa cgttcgagag aaatagttta agtgtgtagc
     3001 tttcataggt tcggtctcct ttttactttt caagcaactc ttcttccagt gctttttgaa
     3061 gtagttgaga gaagtttatg tttttctcct tccctaaaag aactaaccat tccggaacac
     3121 taacattttt tctaattgtt tttgtattta agcttcttcg gaaaagttta atgtttagtt
     3181 ggatgattac taccacttgg ttggctttca aagttaattc ttccactgct gatggtttag
     3241 gaaattctgc atctggtaca ctatatagcg ctaatcctaa agcatcctta gcgtttaaat
     3301 atgcttcttc aagactattt ccaaaggtag atatttccgg taagtcaggg aaaacgatgt
     3361 tataagcttc tccttctttc tcgaagattg ccggataaaa aatatttttt gtcttcattt
     3421 cctcgctcct tttctagtgt aagagaggat agtatgccta ataccaaata ggaaggcaag
     3481 gctgttaaag ccctgcttgc ttccagatat tagcttctgt tcctagtggt acatcaccat
     3541 tgtggatcgg aacaacgacc gttcttttag tcttggaatt ataaaatttt gcatgactgc
     3601 cagtttgtcc agtctttttg aagccgttag atttgagaat ttttacaatt tctcttgctg
     3661 tcttaggcat agctaccctc cctttacaaa atctattata cacattgttg tgtatggaat
     3721 caatagaaaa agaggatgtt ttattgcaaa actaataaaa tgatttattg gttttttcag
     3781 caaattgaat gttattagcg ttgcaaatat tcatgatttc ccaagtatca tcagaagtat
     3841 aggtatctaa gaattcattg acagttacgg ttgattcgta tggttcataa gtcaaagcgt
     3901 tctccaagtc ttttgatgaa ataatcaaga ctttttcaat ttcctcccct tcttcatcta
     3961 tgaaaacata ttctaaagtg atttgttctt catcggtata attcatattg tgttctcctt
     4021 tcttgtacta agaaaaatgg atatttccaa ttgcatccca ctcggcacca ttgtggtata
     4081 ggacgtgacc gagcgttacg cctttgaaat gttcttctaa atacatccaa acgcattcaa
     4141 ggctagtacc tgctttgaga ttgtagaagt ctgcatccaa taccttttca gaagttatcg
     4201 gatcaatgcg agttggtact tcaataaatt cagtccaaat ttctgcaaaa gtcttacctt
     4261 ccaaagtcca cactccctat caattaattc caaaagcaaa tagagaaatc aagcgaccgt
     4321 caatagtcac ttcaacaact gaaaatgccg ttgaaacagt tttaacatct acactttcca
     4381 agttcccgat gaaggaatct ttgtgcttta gaatagcttt ctttagctgt ctgttataca
     4441 cttgccgact gttaaacttt ttgaattctc gatccttgat cagctgttta acgatccctg
     4501 cattaattgt ataactttct gcaatagcaa cgtgagtagg ttgctgtaaa gtagctgtta
     4561 gcatcttgtc actccttcct aaaagttaat tagcttgttt acaaatacat tatacacatc
     4621 ggtgtgtata atgtaaagag aaaaaggtgc taatggatca gctgttctaa acaaaagaaa
     4681 acgataaagt aacaaataaa gtcagcaaag ttaaaaagga tgaaacggct agtccggttg
     4741 ctttgcttgt gtgattttcg gctttcttat agatcaaata ggaaattatc agtgatattc
     4801 cagtaaagaa taatgtacta gctgtcacta aaagaaatag gttaatcata cgatacactc
     4861 cttctgtaat attggtatca gatacgttta aatgcgtttc tgagctgttt tggatcagat
     4921 ggtgatgttt ttagccctac cctaaaaaca aagcccagaa aagaatttaa aagcgttcgt
     4981 acctaatacg tttatattca tatacatata cataaacata aacgtgtata cgtttatgtt
     5041 tatgtattta agttttaggc tacttcctta acatctcctg ctgatagtcg agtgaaccga
     5101 gtgagtgatc catcttgtag tcgaaaaata aagctacgtt tgagtttata ctgatagaca
     5161 gcgacatacg atccatccca gattgagtaa gttttacctg caactaataa gtcatcaagt
     5221 tttggtttat tgctttcttc tttaattcta tctagttgtc gcttaaccat tttttcttga
     5281 tcaccgacaa agaatttcaa ccattttttc ggaattttct taaagtaagc aggactgtca
     5341 tttaagaagt agattggttt gtacatttca tctcgaaaga tgaagctgtc aatatcgtag
     5401 gttccttctg catctttgaa aaggataatt ccatagatct tcgttacttt ggtatctacg
     5461 atttccaaac catttttggc gtaccctttc ttttcttcga tcacttcatt tgctgatgaa
     5521 taatcaccat accatcccat aaaaaatcgc tccttttgaa agttaattag cttgtttaca
     5581 cttatattat acacaccggt gtgtataata tcaagaggat attgttcttt tttaggtaaa
     5641 gtgggatcgt aaacagtgat tttgttgggt taacaaaaaa acagacaagc cttatcagct
     5701 tgtctgttac ttataaactt cacttctgtg accaatagac aatataagaa ttgtcacttc
     5761 atgctcgcta atatcagcta ttatacgata tgcacctacc ctatatcgcc agtagcctga
     5821 tttattagct gttagacctt taccatgctt tcttggatca tcagtgccta caagattttt
     5881 ctcgatccag tttaggataa cctttgcctg aaacttatcc atttttttta gcgctttttg
     5941 agcttcacgt tcatatctta cttggtaagt catcttataa gcctaactct tttagcatat
     6001 cttcgtggga aatactttga tcgttttcct catgctcttt cattagttgc ttgtaggtgg
     6061 caaagtctac taacgtttct gctgtttgca gggaattact acgcataaat tcagaaatgt
     6121 tatctccgtt gaattcagca actttttcaa gaaaatcttt ttcttcttta gatacacgaa
     6181 tacttattac tggcatagtt tatcaactcc ttttcaatta cattgtacta cgattgtatg
     6241 acaaatgcaa tagttatata tttgaatata tacataaaca taaacatata catatatatg
     6301 tttatgttgt agaatataaa agcaaatata aatataaaca cgtttatgta tataaacata
     6361 tacataaacg tgtttatagt gtataatgga gttacaaaag gttaataagg gaggtttaat
     6421 catggaaaag aagttgtcaa tcattactgg aaacattgaa aaaggtggaa caggtaaaac
     6481 aacaacaatc tacaatctag gtggatatgc tgtcgaaaat ttaggtatga aggtgctgta
     6541 tattgatgaa gataagtcgc aaaacctatc aaaacgattt cctgatgctt tcagtaaagc
     6601 gaaaagagag aatacaatca atcatttata tcttcatgga tcagctgaac ctttagctat
     6661 aaatgagaac atggatattt tagtagcagg tgaagattta aaacaagttg agatgcaaat
     6721 gcgtgatatg cctaacaaca gaaccctact gtttagatgg atcgttgtta actatgaaca
     6781 gcttaaagag aagtatgatc taattctgat tgatactcat aatgatgcag gtctactagc
     6841 tcaaaatgct tgggctgtat ctgatcttgt cgtaggcatt tctgatccat ctaaagatgg
     6901 ttttgaagct ttgctgaagc ttggtaaaga tattgatgca ctagcaaaac agttgattga
     6961 tattaagact ggtgaaacac tgatggaaac taaatacgct acgatcggta gcaggatcaa
     7021 acataatact aattcaagtc gtgagttcct agaattggta ccaacgttat ctaattatct
     7081 tggatatgta caagagaagg aattgcttaa tgcaactagc ttagaaggta agaatattgc
     7141 tgagttcgct aaggataaga atatctatcg taagaataag gatttctttg aaagcacgtt
     7201 tacgctatac aacaaaataa ttgatacggc acaagaataa ggagtgattg aagtgaaaga
     7261 aaatagattt aaaaaattcg ctgaagctga agtagaagaa gttcctgaag tggttgaagc
     7321 agttgaagaa cctaaacaaa cacctaaaga aacaacaagt tctttggtag ctaaactaaa
     7381 aagtgatagt tctgaaaaga agaaaaatta tcaattcacg cttcaaccat cacaaagaaa
     7441 gaaattatct aaaatcagcg aaaagttagg aattcgatca gattcagctt tacttggtga
     7501 aatgatagat aacttgtatg atgaagtttt tggtgagtga tcagtttaaa gctgatcact
     7561 ttttttattg gaataagcgg ttaggtatag cttaactaaa tttcgtatac gtgtttatgg
     7621 atatatacgt atacatgttt atatacatgt atacgtatat gtttatgttt gtatttgtac
     7681 ataataaaaa ggctaggtat tgtaacctcg cctttttatt tttaaagtaa gtctttgtta
     7741 cttggtgcat tgaacccttt tagcattggc ttttctttct ttgctgaatg atcggatgta
     7801 accgttgaag gatgctcttg ttgaataggg ttatgatcag ttggtttctt ctttacttct
     7861 gctactttgc tatcgtttct atcgttgcct gcttcaataa ttgtaacttc attgttcttt
     7921 acacgccgat ataggttcaa agcttttttg atcgtctgag agtatttaac gcctagtgtt
     7981 tcatattcgg attcaagaaa ctctgctaaa ggtaactcgc taaccttttc tctattgatt
     8041 ataactacat aacgctctga ttttccctta ttcatgttga tctccttcgc tgttttattg
     8101 agttaaacca aacttgtaaa agccttttac attagaaaat tcactgtctt caacaaacaa
     8161 ggtatgcgga attttatctt ttacaagttg tttgtcgata atagaagctc caccaccagt
     8221 aaagacaact ttatctaagg ttgtgaagtt tttgaatgta gctaaaactt ctgcaacaac
     8281 catattggtg tatgttttta tttctctttc aactacttct gaaatatcaa aactttcatt
     8341 tccgcttggc ttaaatagat atacgccttt ttctttgcct tctcggataa tcttttcaag
     8401 ttggaaagca ttaggactga tcggaaggtc aagcatattt acgatacgat tatataaagt
     8461 aaaaatgccg gtatcctttt gttcacgatt gttttcgtca atttcaaaat tttggacaac
     8521 atcaaaaagt ttcgtgccac caccaatatc aacaaaaccg atcttctgac tgatcaatgt
     8581 ttcatcttgt acactaccat catcagcaag taggaggtta tatagtgttc caatcggctg
     8641 tggcagtacc caaacggtat ctacgtttat attataagcc ttaccgtcaa cttcaactga
     8701 atgttgtcgt gtggcttgtt gcatcacgta tttgacgatt gtttcggtat agtcggatga
     8761 aggcagacct aaaactagat taacggttaa aacgtcatca gattgatctt ctaaacgtgc
     8821 taggttagct aaagagaaag ataggatatt tttgtaggtt tcctgcttgt atctgctgat
     8881 cccaaaacct agtgattcta tcacgtagtc atcttttcct agctcgtgta tatccttccc
     8941 aaaataatag ctttcagtag tgtttactag gctatattca gaaacgttta gtgatcgttt
     9001 taatgaggaa cctatattag aaaaacgtcc tgaattaatg atatttttgt tcaaaatacg
     9061 agagggaagt gagagtggag aaacgtgtgt actcatttct tttactgatt tattccctaa
     9121 gtcaacggaa aacaataatg gttctttcat ggtatgacct ctttctgttt gtgtttatgg
     9181 tatttcggta cgaaaatagt ttacacctat ttactattag ttgcaactgt ttataagaaa
     9241 aaaatgcaat cataaaagtc taattaatta taataaagtg taaataaaaa ggtgtaaata
     9301 aatgcttgca aatttatctt attatgatat tataaaatca taaattatat taaggagtga
     9361 tcctgatgcg attgagaaga aataagaatt acactaccat tattttagaa gattttactg
     9421 atcaagaatt acttattgtc ggtaaaacaa aagcagattt tatcgaaaag ggaaatgctt
     9481 caatagtaaa agcttcaaag gttgctgtcg aaaaatattt tgattcttta gaagaaagtg
     9541 ctaaggaaat ttttaatgga gagaaagctg gtagctatga gttagacggt attagattta
     9601 gcttgatccc aacaactgaa aatattattc cgaagaaatt atattatatc ttttcatctg
     9661 aaaaacatac ttcaatgaag ccttgtattt ttgattggca atggttgatt gatgctgtat
     9721 tggatgtaaa atgttcaaaa tacaatatta ctttggggtg ttaattatga gtatgctagt
     9781 tagaaaatta ccattatctg taagagaatt tctattttac ttggtctgct taatcgcatc
     9841 agggtttaag ctgatcgaac atatttttat ctttgcttat gtattagcaa tgatcgcatt
     9901 tttatctggc ggttggttaa ttatttattt gttaagctaa gttttagatc aaaaataatt
     9961 agtaaattaa acgataaaat aaccgttaat aaatgtaatg aaaggacaac gcctaacaat
    10021 cagcttgtaa taagattata aaataacata atatcaaaaa tagtgctttt taaaggacaa
    10081 attaagtgta tcaacataaa cgtgtatatg tatatgttta tgttgatact ttttttgtct
    10141 taatttttta tttttaaaat tctatttact aatgctgtat agatagtgat cagaaaaaac
    10201 aaaatagcga gattaagcta tttattagtt catatattat attcagatta atatgatcca
    10261 gtattaatct gaaggcaaaa ccaatatatt tctacggact cttgtaaaac tatttaaatt
    10321 ttcctgctaa taaaataacg aaaatagaat atttagcttt tttataccat tttacgataa
    10381 catgatttca tttgtaatat taaaacattt aaatttttaa ttattctttc aaattagatc
    10441 aattaatatc cttataaaag agcataataa atggttttta catcgttatt tattgatatt
    10501 aatctcatcc aaaaaaaatg aaaacgcata cttttatggg tgattgaatg tgattttaat
    10561 acatatacca attaaaagaa aactttttag atttttactt acaaatttat aaaaaaacaa
    10621 gataattgat actttgattt tgcgaacatt ttatcttgcc gcctaattca gctttaatat
    10681 catgattata tcagtaacaa atcgcttttt tagctaattt aagcatcaaa taattattct
    10741 taatcaggga aaaaataact aataaatgga ttttgggaat aggtaaaaac ttgatcggtt
    10801 tctggctaaa aataacgtga aaaaggaaat ataaaataac atggagcgtg ggaggaataa
    10861 agaaatttta cacgaaaggt gcgcagggat gtaaagaaac ttaacacgaa acaaaagt
//
LOCUS       DAFCNS010000083        10543 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFCNS010000083 DAFCNS010000000
VERSION     DAFCNS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.085"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            104..1147
                     /locus_tag="LNE23_002342"
     CDS             104..1147
                     /locus_tag="LNE23_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBK6932168.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1320..1544
                     /locus_tag="LNE23_002343"
     CDS             1320..1544
                     /locus_tag="LNE23_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932169.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1547..1804
                     /locus_tag="LNE23_002344"
     CDS             1547..1804
                     /locus_tag="LNE23_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932170.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            1906..2259
                     /locus_tag="LNE23_002345"
     CDS             1906..2259
                     /locus_tag="LNE23_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932171.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            2312..3106
                     /locus_tag="LNE23_002346"
     CDS             2312..3106
                     /locus_tag="LNE23_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932172.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3135..4253
                     /locus_tag="LNE23_002347"
     CDS             3135..4253
                     /locus_tag="LNE23_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932173.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4286..4606
                     /locus_tag="LNE23_002348"
     CDS             4286..4606
                     /locus_tag="LNE23_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932174.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            4861..5550
                     /locus_tag="LNE23_002349"
     CDS             4861..5550
                     /locus_tag="LNE23_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HBK6932175.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            5587..5754
                     /locus_tag="LNE23_002350"
     CDS             5587..5754
                     /locus_tag="LNE23_002350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932176.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            5774..7402
                     /locus_tag="LNE23_002351"
     CDS             5774..7402
                     /locus_tag="LNE23_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932177.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            7453..7824
                     /locus_tag="LNE23_002352"
     CDS             7453..7824
                     /locus_tag="LNE23_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932178.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            7909..8109
                     /locus_tag="LNE23_002353"
     CDS             7909..8109
                     /locus_tag="LNE23_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932179.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8243..9469
                     /locus_tag="LNE23_002354"
     CDS             8243..9469
                     /locus_tag="LNE23_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932180.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10066..10461
                     /locus_tag="LNE23_002355"
                     /pseudo
     CDS             10066..10461
                     /locus_tag="LNE23_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
BASE COUNT         4103 a         1281 c         2100 g         3059 t
ORIGIN      
        1 caacaacgat tatgatctta aaacaatcgt ttatgtaatt ggcaatctta cgaataattt
       61 tcatgcaata atgacaataa aaaatgaaaa ggggtaaaat caaatgaaaa aatttttagt
      121 aggaattcta ttcacaatgg ggctagtatt attatcacct tcagtttcag cagaaagctc
      181 tatttccatg tatcgtatgt ataatcccaa tagcggagag catctataca ctagaagcgt
      241 tggagaaaga gacaatctga aacgttatgg atggcattac gaaggtgtcg catggcaagc
      301 tccaacaagt ggtactcctg tttatcgttt gtataatcgg tataacggtg aacattttta
      361 cacagttgat gccggagaaa gagataagat ttcacacaca ggatggacgt acgaaggtgt
      421 tgcgttctat tcctacaacg gagcaaatag agttcctgta acacgtttat ataacaaata
      481 cgtgaattgg catcactaca cgttagatca aaatgaaaag aacgtaatta gtaaacaagg
      541 ttggaagatt gaaggtacag gctggtatgc aatgccaaac agcgtaccaa atcctacacc
      601 tgctccaagc actgaaaaag tgttgaacgt tcctgttgta tggcaaggaa atacaatgtt
      661 atgcgaagga gcttctttat tagaaggttt acactacaaa ggtgtagcca caaatcaaag
      721 cttaaccagt tttgtaaact atatgccaaa agctaatgac aataatcctt atcatggtta
      781 ctcaggagag tggcgtcata acgttaatgg aacgtatcaa ggcatgatgg ctgacccagt
      841 agttaaatgg gcaaaaaacg ttggcggtaa tgccgtcaat atcactggtt caggaacgaa
      901 tggaatcaag aatgaaattc gcaatggtaa tccggttgta acttggatta cttataacta
      961 tgctacgcca gagttcaaac aaatgccttg gggtcgtgca gtatggaatg gacatgtagt
     1021 tctcgtggat ggcttcagaa atggtgcata ccatgttgtt gaccctgtat ttggcgcaaa
     1081 atggatcaat gcaagcacgt atgagagagc atttaatgtg actggaatgg cggttgctgt
     1141 acgttaattg atagaattat caactaaatg aaataagagc ttaattactg aaatggtaaa
     1201 taggctctta tttttttaga ggaaattttt gtaatacgaa tctataacaa aaaatattga
     1261 atgtagtaca caaaagtggt atattaaaat cgtagagatt atgcgaagag gtgattttga
     1321 tgaaccaaag aagaagaaaa aggttagaag aaataagcga gcgactttcc gagctaaagg
     1381 atgagttaga agtaattcaa gaagaagagt acaaagaaaa tatacctaaa aatctacagg
     1441 gatcagaaag atatgaaaaa gcagaagaat ccgttgatca acttgaagaa gctgttgatg
     1501 gtttatccga catattagaa ttgattgaag atatagctag ctagagatga ttaatgaaga
     1561 tttcaggagt gagaataaaa tgattgatat ttcactgata gagaagttac ttttctcaaa
     1621 taagtacacc gcaaaagagc tggctacaag atggcaaata tccgaatcag ctattaaagc
     1681 ctatcgtcaa aataagggaa gcgtaggatg gcgtgattgg aaagggatca gcattggaaa
     1741 agcagaagac ataatgaaac gtgaaggact tcgatcctat accacttctg aaaaagacaa
     1801 ataacttaat gtttgaaaag ataaaatatc tgtcttaaag ctatgtcaaa ttcaaatggt
     1861 gaatgtttgc taaatataat gcaaaaaaag gaagggaaat attaaatgat aagcgaaaat
     1921 ttgtcttatg agaataataa acatctagtt aaagaaaata agaaagtaga agattataag
     1981 aacatgtata tagaaatgaa aaaatcacca ctgtgtattc aatataaaaa tgataattgg
     2041 aagcaagtag tcgaatttgt aaaacctcat agtatacgaa gagtacgaag ggggaagaaa
     2101 aaaagtgaac ttgtaaatag cggaagagcg gtaattagag ttataaccaa agattatcca
     2161 gttgaatatg gagattatat tttaaggtta agttatcatg cttttgttgt gttgaaagaa
     2221 gagcatttca acgagttttt caatttaaaa agtaagtaat catatttttt tgtagacgtg
     2281 tataaagtta actaaattgc gaggtgatga catggtagca aaaaagaata ctctaaaaga
     2341 agcttctgaa aaatatcaag atatgaaaag acgaggatta ataccgaaag acaaaaaagg
     2401 tatatggaga gaatttgtta aggaagtcag tagtgatcga agtggcggtg actttgttgg
     2461 acgatataca gcaaaaggct atagcaaacg attaaggatc actgttgaat atggaacgaa
     2521 tgaatcagct acagagctga agcaaaggta cttagataca cgaagggaag cttcaaaaaa
     2581 atataatgat ttcaagagca gaggtctgat tcctaaaaag aaaaagggaa tttggcgtga
     2641 gtttttcaag gaagaagata gcaatcggaa taagggagac ttcattggac gatacacagc
     2701 aaaaggctac actaagaatt tagggatcaa ggttgaatac ggaactggtg aaagttcatg
     2761 ggaattgaga aatcgctatt taaatacatt gttagagaaa tataaagcag aattattgga
     2821 gttgttcgat gttaatgatt ttatcgagat ggctgaaaag tatggtcatg aagaaggttt
     2881 tatgatcaat gacattatgg ttattgataa ttttacggat ttattagcac ttgcgaaaaa
     2941 agcaagggat ttaagagaaa cgcagatgta tctattcaga aataaaaacg gaagaaaccg
     3001 gagaactgaa tcgagtgaga ttgaagatta ctacgatgcc tttacacacg atctgggagc
     3061 ctttgtagac gagataagtg gtgtaatgaa caaatatagg gtctagaata aaaaagccgg
     3121 agatgagcag ataaatggga aacaatagca tatacacacc tgaaatgcag aataaaatta
     3181 aaagacttgt cggattaggg tggatgacaa gaggaaaatt agattctttt agtacgatca
     3241 aatatctttt gatggacatt taccgtcaga acaagcaaac aggaatacat tttttgggct
     3301 atgataagcc ggaagatgca cctttctttg ttccaagttc agcctataga gccagtacct
     3361 ccggtagcat gtataacttg gatttgtttg aggaaggagc aaagatgatc gaatatgatt
     3421 tcaacgaagc ctattcgagg atcatgagaa attatccttt gccgtcaaac acttatttga
     3481 aagcaaaata taatacagat caagctattt tgagagcgat aaatagacca aaagtaagac
     3541 cgtatgaaaa tttaagaacg tatatgttct ttttggttaa ttttgaagga acagccaaag
     3601 aaggaacata tgctaatcca aaatcacagt cgatgtttgc ggattatggc gaaaatccca
     3661 aaacagctaa tgagcctatt tggttatcgg agattgagct aacacttatc cttgattatt
     3721 atgatattaa agagatggaa gtcctagata gccacacatt tttgacgaaa aaaggattac
     3781 tcaaagaata ttttgaaaga atcgagccat taaaaactga tcctgatacg ttgaagcttt
     3841 ataaaaatct tagaacgaaa gtatttggtg ctatcggaca aaaatcatta agagaaggag
     3901 aaacaagcac cttcgataaa tacagtatgt ataatcgagc gtattcctcc atggtagcag
     3961 gcgtatttag agatcgtatg gttagatatg aacagaaata cgttcatagc gcatatgggc
     4021 tattaaaaat tcggactgat ggattgtatt tcagatccga agtaccagag tttgaagagc
     4081 tgtataaact tggagtagtt aagaaaaaag ttcatacaat atccaaagaa gaagtcgaaa
     4141 aagctttaga atttaaaaac aaaggatttg aaaataaggg ctcctttggg aaatctaaag
     4201 atgaagttaa gggtgttgtg atagacgatg atttcgagtt tgattttgac taaatactct
     4261 ttggaaaaga tggtaggagg taaatatgaa gaagaaaatt gtggaaggaa atgtaggtgt
     4321 tattattcta aaagattcaa ctgactatgc taaatcaatg gcactaaaag acgaaaaagt
     4381 tgcacgcatg gataaagatt tagttgaatt tctgatggat gatacagaaa gattaaaagg
     4441 ggatcagttt ttgagtttaa tgaaagacaa aggcttaaac acatatggtt tatttgatta
     4501 taagaaatta gctatagagt gggtaaaaga aggagaaata ttttttattg aagaatggga
     4561 aggtactgag aacttagttc ttcatcagta tcttttgaaa gcttaatcta agtaaaacag
     4621 ttctgactag ttatgcttgt cagaatttca aaggaagttt tccataacca tcatcattca
     4681 aaaataaagg atttgaaaat aagggttcct ttgggaaatc taaagatgaa gttaagggtg
     4741 ttgtgataga cgatgatttc aagtttgatt ttgactagat ttttttgaaa ggtttaagag
     4801 taaacaaggt aagaactaag atcatgaaga ttgagagtaa agaagataga ggacgaatgt
     4861 atgagaacac actatattta tttagaagat aaaattgtag atgaaattcc ggcaaaaagt
     4921 cgagaacaag ctagaaaatt atttattgaa aaatacaaag agatcaatgg agatagtgaa
     4981 gttccaaaag tggtcgtaac agttagaaaa aggcaaacaa aaaagagaag tacgtttcat
     5041 gtcagccctg atatatataa tcttatagag cgacatagaa gtgaagataa tatttctatc
     5101 tctcaatata ttgctacagt tattgatgaa ttgatttcag gaaagtatga ctatgctttt
     5161 gattttgagg gtgaaaagga ttacaaaagt gttgctgtct ttcaatctga ttttgagaag
     5221 ctaaaaaact atgctggcag gagaaatgtt aattttgcaa gactactaga agagttctac
     5281 tccgaagagt tgggagtagg aaatgaatac aagagagaaa cttatgcgaa attgtctcat
     5341 aagttatccc aagcgcttaa agaagtgaca gaagttaaag cggaactaga tgctaaactt
     5401 gaagaagtga aaaaatatca aacagagagg aagaacaaca tgaagaatca agtggaattt
     5461 gacgaacaga aagaatttga aaaagcaaga aaacgctata acaaaattat gaatgaactt
     5521 agctatgaac acttaacagt tcatgaataa ttgagtgagg gtacagaaaa atatacgatc
     5581 gcagatatgg ttaatgaagt ggaaactctt ttagcttctt tctttgaaaa tggtcacgta
     5641 aacgatgaat ggcgaaaaga agaacctaaa agggttaacg cttatattgc tcgattaaaa
     5701 aggtttattg ataaatataa ggagaatagt gacaaatttt ctaaagataa ataaggagag
     5761 agtgtgatta catttgccag aaatcgaagg aatattttcg ccaaaaatag gtaaaaaaca
     5821 gttagatttc agcagttttc cgagcgtaaa tagagattat aatctaggtg atgaggtaca
     5881 gatactaaat attgatttca aagaactaga cttcgctgtt gctgtttcca acaaacccaa
     5941 ggcattggaa cgagtgaaaa aaatatacca agagaatgat caaaatatca aaaatatgat
     6001 caaaaagtat agacgagata attcaaaagt tgaagacgtt ttattcaacg gtacggcaaa
     6061 acatgatttg aatgtttata tggcattagg agtggctttg tactgtaatt caattaatca
     6121 gacagatatt ttggttaata tcgtgaggaa tagctatcaa aaaatattaa aaataagtag
     6181 cgaaacaaac ccttattatg caatgaaata tcttggggat aatttattaa atgataaaaa
     6241 attgatgcac aacactgaag agctgttatc taatttggct gcgattaact ttctattcaa
     6301 aacaggttct gaagattatt ttatagataa cgtactaaaa ggtgcttacg gaacagcatt
     6361 ttatccgctt tacaatatga aacagcataa agccgaacaa aaggataaca aacttgaaat
     6421 taaaaagatg aaagaactat taggtatagg atttgtaaaa cccaattcaa aagtcactat
     6481 tgcttcgtta gcaactacta acaatgatgg gaaaatagta aatgggtttg acgattcaga
     6541 tatagatata ttgtcaaaat catatttttc tttgtatgga gattttgcag atttgatggg
     6601 tattgatagt tacttagtca ataattttga gatcaagggt agcgagctga atgatattgt
     6661 gtatttcgtt ttaatctctc tatatgcaat tgatcagaat accgagtacc gacaaattga
     6721 ctatttgcta gttttttata gtttgttata tcagttcgtt ttaactcggt tgtttaaaaa
     6781 tgctaaggaa gaattgatcc tgaattctga ttatgaaata gcaaaacaaa ttgcaagtat
     6841 tagaaaagaa aacaaatctg aagtaatcaa attaagaaaa gaattaagta ttgcgagtaa
     6901 agaaaacggt cggttagaat ctttagtcaa atcggcaaaa aaagaagagg aacttttgaa
     6961 gcaagagatt aaagaattaa aaggaatcag aaaagaactt gaacagctga ggtttgaaaa
     7021 taaagtatta aagcaattgc aggagcaaga acatagacaa tcattaaatg gtaaaactga
     7081 agagccttca ctgtctgaaa tgatagctta tctaaaacat aaaaacattc ttttagtggg
     7141 aggacatgta aattttcaaa ataaattaac tgaactatta ccaaatatta aaatgtttga
     7201 tgtaagcgaa aataatggaa agatacttaa aaatttgaat acgtgtgatg cgatttttct
     7261 atatacagat tacttgaatc atggtctata ttatcgtgtt atgagtgaaa tttctaataa
     7321 agataagata ttttatttgg ataatcatgt gaatatgaac ttaacaatta aacgtattta
     7381 cgatctttta tcacttatat aaagagagaa gtgataaata ggacacaaaa tttggaaagt
     7441 aggtaagtca ctatgagaga actaacttta tcttctaagg aattagaaaa gaaaactgct
     7501 ttattaaacg aaatatttag tgctgattgt ataatccaaa gtttaaaaga aagttcattt
     7561 tcaaaactaa cagatcagga aaaaatttct tatatgctaa gtttagataa tctaggaact
     7621 aaatatgatg aatcagcaag tagcgataag cttttgattg aattagtatc aagtcttagt
     7681 agttttggaa aaatgaaaag atttaatgtg atattagaaa tttttgcttg gttaatccct
     7741 tcatttattt ggttttttta tattttcata gttgaatctg agttaatagc caaggtctct
     7801 cttggactgt taattccgat ataaataggt tcagcaattt actttaataa cttgtataca
     7861 cctattcaaa taaaaaagca gttattacat gaaggattaa gtggtaatat gttaaaaaga
     7921 attgcatggg ataaagataa taaaacagat attgagctta aagaatataa atacaattat
     7981 ttctcgagat gtatagatag aaagttactg tttaaaaaag aaggattaga atttagtttt
     8041 gatgaagatg aatttgaaaa attagtgatg gactggcgta agaatttact ggatacggta
     8101 caattctaaa agcaattgag gtcgaaattc cgatacttga atcatgatct atattatcgt
     8161 gttatgagtg aaatttctaa taaagataag atatttcatt tggacagtca tgtgaatatg
     8221 agcttaacaa ttaaacgtat ttatgatctt ttatatgttt ataaacatta atgaggtgct
     8281 aaaattgaca acaataacta tgacaaaaaa aatagaagta tatgttgatg gatcacaaga
     8341 agaaagggat tccttttttg attttttttg gaaaaatagt agagaatatg taaaagcgtt
     8401 gaatctggta tataaacagc taatgtttga agagttgata aaagatgcaa ttaaagaaat
     8461 ggatcaaggt tatgctgaga gagaagaaag tattcaaaat cggattgaaa agttagcata
     8521 tactgatcca atagatcaaa aaaaacttga taaagcaaaa gctgaattat ctaaattgag
     8581 aaatgaaaaa aataaagaag cgagagaaat attaacgcaa gcgatagggt tgaagagtca
     8641 aacgagaaca cgagatgtag caaaaaaatt agactttgac tttggagatt ttgttgattc
     8701 cgcaaatatg aaagctagta aagattttgg caatgatttt atcggaattg tgaccggaca
     8761 aagaacgcct agaatatata aaaatagaaa caaatatgga ttattacctt ttagaggtcg
     8821 agatattaaa atccataaag atgataagtt ttatatttat ttacctaaag gctttaggtt
     8881 taaagtgaat ttaggtagca agccaagaaa agcaagagat gttgcttcag ttttacaaca
     8941 cattatggat ggcgattaca aattaaatca aagtaatctt atttttgatg gaagaaaatt
     9001 attttttaat ctaacaattt cttttgaaaa aagctataat gaaatggaat tcaagaaagg
     9061 taatgaattg atcgtaaact ttgcagattt gtgtagcggt gaatgtttaa ttaatgataa
     9121 atctgtaatg tcttttggtg atagccaata tataagaaac ttcaaaagta aaattttagc
     9181 aatgaaatca aaagaacaaa gccgaggagt cttttctaag ggcggtcatg gtcgaaaaag
     9241 aaaattagat caagatcgat gggaagcaat aaagaaaaga gaaagtaatt ttgttaaaac
     9301 ttataataat caatgttctg caaaaatcgt taaaactgct attcgcaata aagctcaaat
     9361 tattcgtatc attgaaccag ataaggctta tggcgaatgg gcttactatc aattgaaaga
     9421 acaactagta aacaaaggaa aaagatatgg gatagaagtc agaagttagt aaattaaaca
     9481 atcgttgatt tggcaacgaa atagtgaggg aaaatccact cactgaaggg taaactccgt
     9541 caagttgtgt gaccagacgg aaggacgaat gttgttgagg ttttagaacc atataaaaca
     9601 gcatgtttct caaattttta ggataaggga aattgctagc agtttgtttt agaaccatat
     9661 aaaacagcat gtttctcaaa tgagtacttt aaattaattg atggcgttcc tgctttagaa
     9721 ccatataaaa caacatggat ttaaaacaag aagtaagtca gttctccttt tactttagta
     9781 aggaaaaata aagtattctc aataaaactt aaaataagca aacctttgtt ttaaaatgat
     9841 tgtcaagtta aaaaaatctg ggatcatata aaatagtata gttctcaaat atgattagaa
     9901 accctattaa agagcaatat taagccaact tacttaaatc gtttaattaa gaacaataat
     9961 ctcttttagt cagactttta gtctgacttt tttgtatttc caaaatttat tctttacaaa
    10021 aacgaacata cgtttgtata attacttgtg aatgggggaa taaaaatgtt tgattacagt
    10081 agagaaccgg tgaatgacta ttttttaatc gacatgaagt ctttctatgc tagcgtggaa
    10141 tgtattgaac gaaatttaga cccaatgact acaccgttaa ttgtcatgag tagatcggac
    10201 aacactggtt caggtctgac tctagcttct tcacctactg caaagaaaaa atatcatatt
    10261 actaatgtta gtagagctac tgatttacct tatccattac caaaagaact tcatattgtt
    10321 ccaccacgaa tgaacttata tataaaaaaa aatatggaag ttaatcagat ttttagaaag
    10381 tatgtcgcag atgaagattt attgatttat tcgattgatg aatcaatttt aaaagtgaca
    10441 aaatccttaa atttatttac tatataaatt gagttaaatt gtattcgttt ttcctcctat
    10501 aggcgtatac taaagaaaac acttgaggag tggggaacat gga
//
LOCUS       DAFCNS010000084        10533 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFCNS010000084 DAFCNS010000000
VERSION     DAFCNS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.086"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            21..644
                     /locus_tag="LNE23_002356"
     CDS             21..644
                     /locus_tag="LNE23_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932181.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1094..2143
                     /locus_tag="LNE23_002357"
     CDS             1094..2143
                     /locus_tag="LNE23_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6932182.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2140..3021
                     /locus_tag="LNE23_002358"
     CDS             2140..3021
                     /locus_tag="LNE23_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6932183.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3024..3794
                     /locus_tag="LNE23_002359"
     CDS             3024..3794
                     /locus_tag="LNE23_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6932184.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4073..4927
                     /gene="aroE"
                     /locus_tag="LNE23_002360"
     CDS             4073..4927
                     /gene="aroE"
                     /locus_tag="LNE23_002360"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBK6932185.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5105..6268
                     /locus_tag="LNE23_002361"
     CDS             5105..6268
                     /locus_tag="LNE23_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6932186.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6385..6795
                     /locus_tag="LNE23_002362"
     CDS             6385..6795
                     /locus_tag="LNE23_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6932187.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6969..9197
                     /gene="topB"
                     /locus_tag="LNE23_002363"
     CDS             6969..9197
                     /gene="topB"
                     /locus_tag="LNE23_002363"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK6932188.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9368..9652
                     /locus_tag="LNE23_002364"
     CDS             9368..9652
                     /locus_tag="LNE23_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932189.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9812..10048
                     /locus_tag="LNE23_002365"
     CDS             9812..10048
                     /locus_tag="LNE23_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6932190.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10045..10443
                     /locus_tag="LNE23_002366"
     CDS             10045..10443
                     /locus_tag="LNE23_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBK6932191.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3667 a         1769 c         2229 g         2868 t
ORIGIN      
        1 gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt agttaaatcg agagtacaag
       61 aaatgaatga acatttctca gtgtcattcg cagaaaatcc aagtaagtat tttcgacgtc
      121 ttgtttttct gctcatctta tcattgctgt taacagttgc aatgttttta ttgccggaaa
      181 aaatccgaga agagcaagta aatagtctcg tttattcgtc cataccaacc agcaaattga
      241 aaccttttga ttacagcaaa gctgatcaga aaatcaaaca aagccgggcg atatcggtga
      301 tgttcagcaa accgaacggg aaaacctatc aggacatgct tgatgttttt aatgatcctg
      361 agcttatgga agaattgaat cgaccgatct tttattatcc gatcgtttat gatgtacatg
      421 aactggaaca aaaatataac atacgaacgg atgaagtgac atttattttc tttgatggag
      481 gaaaagaagc aaatcggata actgcgggta agggaggaat aactgatttt gataaggagt
      541 tgatccctga actgaatcga cttccgttag ccaatatcaa acaattagaa gaagaattgg
      601 atagaacggg agtaacttcc caaaattccg agattgctaa ttgagacatt ggcgcgatga
      661 aacggtgggt ctatatagcg ctatttttaa ttatcagaac aagtgatgtt tgatcttcaa
      721 aataacaaat aaaaaattaa aaaatatagt tgacgaaatt caaaaattcg attagactta
      781 aatcaattct taaggaaagg gtgacctgta atgaacttat caaaacgtat ttcagcagaa
      841 aacaaaaaaa attggcgcag ctggcgtaga taagttgtat taatggtgag actttcccat
      901 agatacaact ttttagagtg catttctaaa gcttgtatct atgctagaca tgaacattga
      961 ctgccgcata gattagaatc tatgcggttt tttgtgtaaa gacaatgacc gctttggcca
     1021 ttgtcttttt ttgtagactg gatacttgcc tgattataat aaataaaaag attgactttg
     1081 caggaggaaa aaaatgaaaa acaaacgatt aataacagtt gtagtaatta ttttagttta
     1141 tttagcaggt gcatttattt atggaaaagc ctcaggcgaa aaagaaagta cttcgacgaa
     1201 ccagacaaaa aaaacaacag ttgttggggt attgcaatac gtgagccatc cggcattaga
     1261 tgagatttat cgaggaatca aagatggtct gaaagaagaa ggattaaaag aaggagaaaa
     1321 tcttgagatc aaatttcaaa atggacaagc cgatcagagc aagctagcca caatgagtca
     1381 acagctagta caatctgatc cagatgcctt gattggaatc gcaacacctg ccgctcaatc
     1441 attagcaaac acgacttcat cgatcccagt tgtgttaggt gctgtaacgg atcctgtggg
     1501 ggctggatta gtcgcttctt tagagaaacc tggtggtaat gtaacaggtg tttctgatca
     1561 gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg ttgccaaatg caaaaaaagt
     1621 cggaatcttg tatgcttctt cagaggataa ttcaaaatac caagtagcac aagcagaaga
     1681 agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg gtgccttcca ctaatgagat
     1741 tgcacaaacg gttcaagtga tgagccgaca agtcgatttt atctatgttc cattagataa
     1801 taccattgcc aatgcgatgc aaacagtggt aaaagaagca aacaaagcaa atatccccgt
     1861 cattccatct gtggatacaa tggtggaaca aggtggacta gcaacaatcg ggatcaatca
     1921 atatcaatta gggcttcaaa gcggtaagat ggcggcagaa cttgcgagtg gaaaagaaaa
     1981 accggaaacg acgcctgttt atacatttga tcaaggggat acagtgatca atcaatctca
     2041 agctgatcgc ttaggaatca ttgttcccca atcgatgaag gaaaaagcca aaatcattac
     2101 agatgactcg cagcaagaaa catctaagga ggatgacaaa tgatcgtatc agctatttca
     2161 caaggcatgc tttgggcaat attaggatta gggattttca tgacttatcg gatcttagac
     2221 tttcctgata tgacgacaga agggtccttc ccattaggtg ctgctgtctg tgtgacagct
     2281 attactcatg gggtagcgcc gattgtagct acattattag gcgtaggtgc tggcatgata
     2341 gcaggactcg ttacggggtt actttataca aaaggaaaga taccggttat tttagcaggg
     2401 attttagtga tgtcaggatt gaactcagtt attttgtttg tgatgcgaac accaaatctt
     2461 tctttattga atcatccgat tctacaagac agcttccatc gctttgggtt acctgattac
     2521 tttgacacag tccttcttgg cttcatctct ttgggcatct tgattgctgt actgctattc
     2581 ttcttcaata cagatttggg tcaaggctat attgcgacag gagacaacga aacgatggct
     2641 cggtcgttag ggataaaaac cgatcgtatg aaaattttag gtttaacttt gtcgaacggt
     2701 gtcattgcat tatccggtgc gctgattgcc cagaacgacg gatatgcaga tgtaaataaa
     2761 gggattggcg taatcgtcat cggtcttgct tccatcatca tcggtgaagt gatcttccaa
     2821 gaattgacgc tagcagaaag attgattgct attgttgtcg gcagtatcat ttatcagcta
     2881 ttgattttag tagtaatcaa actagggttt gacacgactt atctgaagtt gttctccgct
     2941 atcatcttag ctatttgtct gatgatcccg caattgaaga aagcactgaa cttaaaaacg
     3001 ggttttgaga aggaggcata gtaatgactg atatattgac aatcaaaaat ggcagaaaag
     3061 tcgtaaacaa tggaatgaat gaagagaaag tactattgga tgaaattaac cttacaatca
     3121 gagaagggga cttcatcact gtccttggcg ggaatggtgc tggaaaaagc acactgttca
     3181 atacgatcgc tggtacgctt caactcagtc aaggatcgat ctcttttaaa gatcagctga
     3241 tcacaaaaga ttcagaagaa aaaagagcac gattcctttc tcgagttttc caagacccca
     3301 agatgggaac ggctcctcgg atgacagtag cagaaaattt gctacttgca caaaaaagag
     3361 gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca aaaagaagat tttttcagac
     3421 tatgtcaaga agtcggtaat ggcctagagc aacacttgga tactccagcg ggtgaattat
     3481 ctggtggtca acgccaagca ttaagcttgc tgatggcgac gatcacagct cctgagttac
     3541 tattattaga tgaacatact gcagctttag atcctaaaac agctaaagca ttaatgcgat
     3601 taacaaatca gcggatcaat gaacaaaaat tgacttgtct catgatcacg catcgaatgg
     3661 aagacgcctt gaaatacgga aatcgtctga tcgtactcca aaaagggaaa atcgttcaag
     3721 atatttctga aaaagaaaag caacatttga caatgggcga ccttttgcag ttctttgaag
     3781 aagtagtgga ttgatttcga cagaaaaatt cctttcagta gaaggacttg aaaactcatt
     3841 gacatcttcc tttgattcat cctatactta aaacaataaa caattaaaca tatttgggtg
     3901 ttttgtagga tcacgtagtc aatgatttta acaattaaat agaatattct ttgctctgac
     3961 aagtaaacga tgagaaaaga atttgctact ttgccaccta gacgtttcgt actttttcgt
     4021 atcaaacgct aagtggcttt ttttattgaa gtaagaacaa aggaggaata agatgatttc
     4081 tggacaaacc aaattagctg ggctctttgc tgatccggct tctcacagtt tatccccatt
     4141 gatgcacaat actgcttttg aagcaaacaa catcgatgcg gtgtatttag ctttccaagt
     4201 aaatccaatg aatttgaagc aagcagtcga aagtattcga acttttgata tgttaggtgt
     4261 aaatctttcg atgccgaata aaacagcggt tattccttat ctggatgagg taagccagga
     4321 agcgcaattg atcggagcag tgaatacgat cgtgcacgaa aagggtcgtt tgattggcta
     4381 taataccgat ggtgcgggct tcatgcaatc cgtgcaagaa gctgggattt cgatcaaaaa
     4441 gaaaaaaatg actctactgg gagctggagg tgcagcaaag gcagtcactg ttcaagctgc
     4501 gttagacggc gctgaggaaa tcgtagttta taaacgaaac aaccaaacat tcgctcaagt
     4561 aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt aagataaaag ttcgtgactt
     4621 tgccgatacg catcaattaa gaaaagactt gaaagaaagc tgtcttctag tcaatgcaac
     4681 agatatcggg atgggaaaga aaatcaatca gtcaccgctc ttagatataa gcatgatgca
     4741 tgagaatcta gcggtttttg atttgattta tgcacctaga gaaacacgtt tattaaaaga
     4801 agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc atgttgatct accaaggagc
     4861 aatcgcgttt gaattatgga caaaggaaaa gatgccgatc catgtgattg aaaagctgtt
     4921 tgattaaaaa aggataatag ataaagatct tttattctcg cataaacggt ctcaagaaaa
     4981 gtaatccttt atgtatcgaa gcatttattt cgttattttt tattaaatta tcaaaaaatg
     5041 ataggctttt gtcgattggt tatcgtatcc tttatttatc agtaagaaac gaggaggaac
     5101 cacaatgagt cggatggatc gctataagga catccataag aaggcgaaac ctctgaagcg
     5161 ggatacgggg agtatttttg ctagaagaga acgaaaacga acgcgtatgg aaaatgagga
     5221 agatgctacc cgtatttatg aaagagagca gcattcattt gaacatttga accaatacga
     5281 gcaacagata tacagagatg caccaagagc agaagaacaa caaaaaaata aaaaattttt
     5341 caatcggcaa aagaagaaca aaccgttaaa acctcgaaga aagcgatcgt ggaaaaaaat
     5401 cattctatcg attttgcttg ttttgatcct ttattcaggt atctcatttt ttattggtaa
     5461 atatgtagct gaacatgaca caagcttaga ggcagcgact tcagaaacct ttcatgggca
     5521 aaaagcgacg aatggtgcgc ataatatttt gatccttgga agtgatacac gcggagaaga
     5581 tgccgggcga gccgatacga tcatggtctt acagttggat ggtccttccc ataagccgaa
     5641 actgatttct tttatgcgtg acagcttcgt tacgatccca ggggtaggac aaaataagat
     5701 caattcagct tatgcatacg gaggagccga tctagtgcgt caaactttag tagaaaattt
     5761 tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa tcgtttgaaa aagtgatcga
     5821 tgccttattc atgaacggag tgaagatcga tgcagaaaaa gatctgaatc tggatggtgt
     5881 agatattaaa aaaggggtcc agaagatgga tggacatgtt cttttgcagt acgcacgttt
     5941 ccgaatggat gaacaaggag actttggccg tgtcagacgc caacagcaag taatgaacgc
     6001 tattttcagc caattgaaga atccattgaa tctgatccgt gcaccttatg cagctggtaa
     6061 agctttagga tatacatcca cagatgtcac tgcttggttc ttgacgaaaa acctcttttc
     6121 gattgcacga ggagctggtg gcattgatcg aataagtgtg ccagtagacg gttcgtggaa
     6181 ttatgggcaa agtgattatg ctggcagtat tcttgaaatt gataatcaaa tgaatcaatc
     6241 tgctattaca gattttttga ataaatgatc ataaaaaaga tcaagaagaa gctgcgcaag
     6301 gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa acgctttctt atggtagatt
     6361 taaccaagag aggagctgtt tacattggat atctatctga acttcaaaaa tgagtcgcgt
     6421 gaagccatcg ctttttatga aaaagttttt cagactgaag catcaggtat caccacatac
     6481 ggtgaaatgc ctggaataga acaaatgaac ttgccagaag aagcaaaaaa tctaatcacg
     6541 aatgcacggc tgaacattta tgatacatta gttatgtttg cagatacgcc agaaggaatt
     6601 ggtcctgaat tagttgtggg aaataatgtt tctttagtca tccagacaag aaatgaagaa
     6661 gaaacgaagc gcctatttga aagcttagct gagggtggta agattgagat gccactggat
     6721 gctgttccgt gggctcagtt gtatgggatg gtaactgatc gatttggcat caactggcag
     6781 attaaccaag agtgacagct aaagatgaat attttctgga aacatcaagc agcaaacgga
     6841 tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac tatacggtat ctttttttta
     6901 ttgccagatg cgaaaatatg ttccataata gagaagtccg caaaaaatta agtaacggag
     6961 gtcagaagat gaagacggtg atactagctg aaaaaccttc acaagcaaaa gcctatgccg
     7021 aaaccttcca acagcaaaag cggcatgaag ggtattatga aatcagcgat ccattgtttg
     7081 ctggtgaagt aaccatcact tatggttttg gacatttggt agatatggtt gctccaggag
     7141 cttatgaaga gcgttgggca aaatggtctt tagaaaattt gcctattttc ccagaaacct
     7201 tccgttacga agttccaatg gacaaacgtg cacaattttc gatcgtaaaa cgagaattgc
     7261 agtcagccga taccatcatt attgcaacag atggtgatcg agaaggtgaa gccatcgcgt
     7321 ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa gaactattta cgtttatgga
     7381 tcaattctct tgaaaaagaa gcaatctatg aaggttttaa gaatctccaa cctggaaaga
     7441 actattttcc aaaatataaa gaagcgcaag caaggcagaa cgctgattgg ctgattggaa
     7501 tgaatggaag tccattatat agcttgctac ttcaacgaaa agggattgat ggcagttttt
     7561 ctcttggtcg tgtacaaaca ccgacactct atatgattta tcagctgcaa gaagaaatca
     7621 aacacttcaa aaaagaaacg tactttgaag gagaaggttt aattacgaca aacaaagggc
     7681 agttttctgg aaaaatcgta ccaaaaaaag catttaaaac ccaagaagag ttaacgaagg
     7741 aaatagaaaa acttggagcg catttaggta agcaaaacgg acggattctt gaagttacga
     7801 aaaaggaaaa gaggttgcat agtccacgtt tattttcgct atctagtttg cagacaaaga
     7861 tgaatcagct aatgaaagca agtgccaaag ctacacttga tgctgctcaa ggattgtacg
     7921 aaagcaagtt tctaagctat ccaagaacgg attcctttta cattacagaa aatgagcatc
     7981 agtatctggt taaacatctg caaaattata aaaaattttt aggaatagaa gaagtacaga
     8041 caactgagat caaagcaaag aaacgatatg tcgatgcgaa aaaagttcaa gaacatcatg
     8101 caattattcc gacaaagacg attccgactc ctgcacgctt tgcgaagctt tccaaattac
     8161 agcaagcaat ctatttgcaa gttttgcgca ctactgtggc tatgtttgct gcagattata
     8221 tctacgaaga gacgaccgta atgacgggcg tacagcaatt aaagctaaaa agcatcggaa
     8281 agatcccatt acagcaaggt tggcaggaaa tcttacgcat caagtcaaaa aagaaagaag
     8341 ctcagactct tcctcctatc gtagaaaatg aagaagcaga agtagaactg aaaagtatgg
     8401 agaaagaaac acaacctcca aaaccctata ccgaaggaac gttgatcaca gcaatgaaaa
     8461 ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat tctcaaagaa gtagaaggaa
     8521 tagggacaga agctacacga gccaatatca ttgaaaatct taagcagaag cattacatcg
     8581 aagtcaataa aaacgaaata actgttacgc caaaaggaat cactctttgt aaggcagtag
     8641 ccaatgaacc attactgacg agcgcagaaa tgactgcgcg ctgggaaggg tacttgagaa
     8701 aaattggtaa acaagagggg acaccatctg tttttttaga gaatatcaaa aaattcatcc
     8761 tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt agacttttca gatgagatta
     8821 gcgggacacg tcagataaaa gcgatggaga agaaaaacga tcaattagga agatgtccaa
     8881 aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc tacatgcttg aatccggaat
     8941 gcgacttcaa attgtggcca acagtagcta aaaagaagct gactaaaacg aatctcagag
     9001 atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg ttttactggt aaaaaaggga
     9061 aattcgatgc agttttagta ttaaaagaag atatgacaat cggtttttct tttccatgga
     9121 tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga aacatcaaga aaaaataatg
     9181 aaaacgaagt agagtaaatg aaaacaatga aatagcggaa tatttattcc gaaaagtgag
     9241 aatataaata ccaataaaac taaaaaatat cagtgaatca agcaaaaacg ttggggtagg
     9301 ctgattttat atagatagca aggagttttt ttatttgaaa aaagtgcatt catttttaaa
     9361 ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa gcatttctaa agtatcctga
     9421 ttacgggacg aagttgttgt cattgacgca caatcaaaca gcatattcac caacgttgat
     9481 tttatcgact ttgactgcag gagctaaaag cgctacttat ttttttcata aaacgacaag
     9541 catgctaaag cgaaagaaac agcgaaaaca attgttggtg gtggtagcgg ctatgatcat
     9601 tatttatttt ctaccagtta ttacacaagt atttcatcaa tattttggtt aagaatacga
     9661 gagtttgtga caaaagtcat gtcacaaact tttttctttg tcattttttc gtaagaaaaa
     9721 gcaaaataaa agtatataca ttgattaaaa gtatatactt ggatataatg tacttactat
     9781 agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa atgaaaaagt ataaaattag
     9841 aaaatctggc aatagcgatg taacaactat cccaccagaa gtaaaagaat tcatgggtgt
     9901 gcagacaggt gacgcaatca gttatgtttt tcaatctgat ggttctgtac gaatgatcaa
     9961 agcacaagaa gaacctgata ttgattcatt agttgattcg atcatgaatc aatatgaaga
    10021 cgcgttaaaa gatttggttg atttatgagg tatctttcga ctaaagaaat tatcaaaatc
    10081 aacgcgaaag ttattttacg tcactcgcca ggcgaaatga tcggtgtaaa agatgcaaat
    10141 gcattagata tggccgtcaa acagccttca caagcagtat tcaatcaaga actctatcct
    10201 gaaatctatg aaaaagcggc gattttagca attaatttag ctaaaaaaca tccttttcat
    10261 aatggcaata aacgaactgc tcttgttgcc atgttgttgt ttcttcaatt gaataattgt
    10321 ccagttgctt tttctcgtca agaagctgtt gattttatca taatgattac cactagttca
    10381 ttggatttcg attcactaaa gtccaagata accagttatt tacgacaaac cgcaataaaa
    10441 tgaacgaatt aaaaacttta tttctcaatt agatcagcat gtcattaagg aatatgctga
    10501 tttttttatg tttaatcata caaaatagtt ttt
//
LOCUS       DAFCNS010000085        10493 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFCNS010000085 DAFCNS010000000
VERSION     DAFCNS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.087"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..357)
                     /locus_tag="LNE23_002367"
     CDS             complement(<1..357)
                     /locus_tag="LNE23_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBK6932192.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
     gene            complement(709..1068)
                     /gene="rplT"
                     /locus_tag="LNE23_002368"
     CDS             complement(709..1068)
                     /gene="rplT"
                     /locus_tag="LNE23_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBK6932193.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="LNE23_002369"
     CDS             complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="LNE23_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBK6932194.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1393..1917)
                     /gene="infC"
                     /locus_tag="LNE23_002370"
     CDS             complement(1393..1917)
                     /gene="infC"
                     /locus_tag="LNE23_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBK6932195.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1961..2086)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2237..2869
                     /locus_tag="LNE23_002371"
     CDS             2237..2869
                     /locus_tag="LNE23_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK6932196.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2871..3776)
                     /locus_tag="LNE23_002372"
     CDS             complement(2871..3776)
                     /locus_tag="LNE23_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK6932197.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3861..4520)
                     /locus_tag="LNE23_002373"
     CDS             complement(3861..4520)
                     /locus_tag="LNE23_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK6932198.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4836..5780
                     /gene="mvk"
                     /locus_tag="LNE23_002374"
     CDS             4836..5780
                     /gene="mvk"
                     /locus_tag="LNE23_002374"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBK6932199.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5773..6750
                     /gene="mvaD"
                     /locus_tag="LNE23_002375"
     CDS             5773..6750
                     /gene="mvaD"
                     /locus_tag="LNE23_002375"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBK6932200.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6763..7848
                     /locus_tag="LNE23_002376"
     CDS             6763..7848
                     /locus_tag="LNE23_002376"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBK6932201.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7845..8909
                     /locus_tag="LNE23_002377"
     CDS             7845..8909
                     /locus_tag="LNE23_002377"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBK6932202.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9158..9961)
                     /locus_tag="LNE23_002378"
     CDS             complement(9158..9961)
                     /locus_tag="LNE23_002378"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBK6932203.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="LNE23_002379"
                     /pseudo
     CDS             complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="LNE23_002379"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10268..>10493)
                     /locus_tag="LNE23_002380"
     CDS             complement(10268..>10493)
                     /locus_tag="LNE23_002380"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBK6932204.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3104 a         2220 c         2008 g         3161 t
ORIGIN      
        1 acctaatgac atcaagatcg gcaacacaat aactgcaata gaaatcactg gtccgattcc
       61 tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg
      121 cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac
      181 tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc
      241 agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc
      301 cgagtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag tagccatgat
      361 gacaaaagcc gaccatcaag ttgtagcctt ttacacagta ccagaccatg aaaaagaatg
      421 agataactaa caaaatacca ataataatat cactcatgtt attcctccgt ccattttgtt
      481 tttctgataa gcaaactaat atatcagtca aactcattct aacttatgta gttagcgctt
      541 tcattaaagg atgaccataa atgttcagtt ttcaattaaa aaaccttaat tatgttattt
      601 tagacttata cgttattgtc ctgtttcatt tgatggagga ttgtaaataa aaaaagagga
      661 aagaacataa cttttcgtta tcttctttcc tctttataat tgtttagctt atttagccaa
      721 agcttctttt gcttggtcag ccaatgcagt aaatgctgct gcatcgttaa cagccaagtc
      781 agctaacatt ttacggttga tgtcgatttc agcaactttc aaaccgtgca ttaattttga
      841 gtagctcaag ccattcatac gagctgctgc gttgatacga gcaatccaca atttacggaa
      901 gtcgcgtttc ttttgacgac gatcgcgata tgcgtagttg tatgaattca ttacttgttc
      961 ttttgctgtt ttaaataaag tgtgttttga accgtagtaa ccttttgcta attttagcac
     1021 tcttttacga cgtttacgag ttactgttcc acctttaaca cgtgccatgt ataattcctc
     1081 ctaataaaaa taaatcttca gattctgtta tttgtacttc ttatttcatt tttgctagtt
     1141 gttggcgaat acgtttgaag tcgccactag aaaccatgct tgctttacgc aattgacggc
     1201 gttgtttttt tgttttgccg tggaaacggt gacttgtaaa cgcgcggaag cgtttcaatc
     1261 cgcctttacc agtacgtttg aaacgttttg ctgatccgcg gtgtgttttt tgttttggca
     1321 tgactatatt tcctcctcaa aatctttcgt tatccgacta tcggctgtcc aacagccagt
     1381 tagtcattca aattacttac tgtcgttttt cggtgccagc gtcaagaaca tgctgcgtcc
     1441 gtccattttc gctttttgtt ccactgttgc aatatccgca gtttcttcag ctaagcgatt
     1501 caagactttt tgaccaattt ctttatgggt aatggcacgg cctttgaaac ggatagaagc
     1561 tttcacttta tcgccctttt ccaagaactt acgtgcatta cgaagttttg tattgaagtc
     1621 attcaagtcg atcgtaggac ttaagcgaac ttcttttaca ttgatcactt tttgtttttt
     1681 acgcgcttcg cggtctttct tttgttgttc gaaacggtat ttaccgtagt ccatgattcg
     1741 cgcaactggt ggtttcgcac caggggcaac taacacaaga tccaagtttg cttgttctgc
     1801 aatattcaat gcttctgctt ttgtcttaac tcctaattgc tcaccatctg aaccgatcaa
     1861 tcgtaactct cgtgcacgaa tgccgtcgtt aaccatcata tcctttgcta tggtcattca
     1921 cctccaaata ttatgagaga aagaaaagct taccgaataa acggcaataa aaaaacgagt
     1981 tctcttacag aacccgcgcg tacttcacct aatagaacca gtgaaaacta tctagcccag
     2041 tgacattatc aactaaggcg agaagcgggt gcttctgctt ttatttctca accattatag
     2101 tatagcaaaa actaatcttt ttgtcaatgt ttttcataca gtttgtcttc cttttctgaa
     2161 aatgattaga ttagcaggct ttctagcagt gattcccgct tctttgctat actaaagaaa
     2221 aacgaggtga acccacatga aaaaaatcga acctattcca ccttctcttc ctcttcttac
     2281 aaagggagag ttttatttag aagatgagga gatcttctct ggtcttctga tcaacggagg
     2341 taaccaaagc tatctcgatg ccaaaaatat ggtattaaga gagagtcata tccaaaaact
     2401 tacgatgcaa aaaacaggtt tacgtcattt tgaatgcagt aatgtcattt ttgaaaaatg
     2461 tgatttttcc aatctggagt ggattggtgg cagtttccac caagtcgtgt ttcatcaatg
     2521 taaattaaca ggtacgaatt ttgccgaaag ttatcttcga gactgtactt tcactgattg
     2581 tattgcttcg atggcttcct ttagtagtac aaacttgaaa gctgtctcct tcgatcattg
     2641 tcaattagaa gacagcgaat tttatgaggt cacttggaaa aacctttttt tgtctgacaa
     2701 tcagttaacc ggttcgaact ggtttcgaac ttctctgaag ggattagact ttacgactaa
     2761 tttcttcaca acaatcgctt tatctcaaga ttctttggcg ggactaatcg tcaatcaaga
     2821 gcaagcactt gtgatcgccc aagcccttgg tttgcagatc aaagactaga tcaacgaatc
     2881 cgccgccaca gtgcaataag catccaaata gataagacca aactgatgat caaggcgatt
     2941 tcccatccga aaacggaatg ggtaaagggt aactggagat tcattccgaa gaatcctgtc
     3001 acaatagttg gaacggtcaa gatcagcgac cagaccgtca aaaatttcat cgtgtcattc
     3061 aagttgttat tcagcaaatt attataagtg ccagagagct ggtctaaaat ctgcgaagct
     3121 aagttcgtca tttcaactgc ttgtcgtgct tcgatcagtg catcgtccaa ctcttctttt
     3181 tccttttctg acaacttgcg ataaatagcc agtgccttga tttgttctaa aagtactaca
     3241 ttttgttttg ttcctgtaac taagtaaacc aaaccgatct ctaaatctga aagttgcagc
     3301 agattcttgt tcgttgtttt ttctcttaat ttcagattaa gccgctgacg ttcactattc
     3361 acttcctcga ttaaaggaaa aaaggaatca gaaatcaaaa acaatgtacg aaacaataaa
     3421 ctgtatacac tcatgtctgg catccgttcc aaaagcgatc gaattagtcg aatcacgtac
     3481 tcggtattcg gcgtggtaaa tgtaaacaaa ccgtcttctt ttaaaataaa agcgatagga
     3541 ctagtttcat aatgattatc gactttttgc tggtgcggaa catttgatac aagcagtaac
     3601 gcttcatcaa aagaatcgta ctctacgcgc gcccgttcat ttttatctag agaataagtc
     3661 agcatctcgt cactaatgcc atattcttct tttaagtttt taacagctag cacatcgttt
     3721 tgctgtacat tgatccaaaa attacggtct tgattaaaat agtgtttttc ccgcatactc
     3781 tatcctcctg acatttgttt ccagtataaa gaaaaaagtc gctcaaataa aggtttaccc
     3841 tttatttgag caactgtaaa tcaaatggat actttggttt ggcgaatctc tgctaatttt
     3901 tcttcgatca ttttcaagac ttcttctttt gcttgaggat cttccacaaa attatagcgg
     3961 tctccatcga tttggatttt tggactttct ttgtattctt cataccattg atcataacgt
     4021 ctgttcaatt ctttgtaata atcgtataga cttgggtcaa aggacaattg ttcatacgaa
     4081 cgaccacgtt tttcgatacg ctctaacatc gtatcaaaag atacacgaat atgaactaat
     4141 aaatccggat gttttttatg ggcagcatag ggaagttctt ccatcatgtt ttccagcagt
     4201 tcatcataca cacaaacttc tgtttctgtt gctcttccta gatctgcatt tagatggaaa
     4261 agcagtgaat cttcataaat cgaacggtcc aatacattat tgtcatcttt cattgcttgt
     4321 ttgatgcttg caaaacgttt attcaaaaaa taaatttgca gcaaaaaagc atactgttca
     4381 gggtttgcat aaaataaggg caagacttca ttatcatcaa tcgattcata aaatgcctga
     4441 ctatcaaagt gttcagccat catttctgtt aaacttgatt ttccggcacc aattgtacct
     4501 gctaaaacga tcacgctcat ttacctccag ctcacttcat cactaaatat tgtgtttaac
     4561 tccaaccttt tttaaatata cccccaatat ctagtagtgt caattgtatt ttttctagtc
     4621 aaagaagaaa aaaagtcaaa aaattcttaa gaaaaaagtg ttttttcttt agaaaacgta
     4681 ttcttagttt tacttctgag atggttttag taaaataaag gaggaatgtt ttttgagacc
     4741 cttagtggac aaaaaaattc gtgaagcagc gcaactcgtt ttgggcgctt tattttttgc
     4801 ttaaaaatca cttttacgtg agaaggagtt agattatggc aaactatggc caaggagaat
     4861 caagcggaaa gatcatattg atgggcgagc acgcggttgt ttatggagaa ccagcgattg
     4921 cctttccttt ctatgcaaca aaagtcaccg cattccttga agagctggat gcaatggacg
     4981 atcaactggt ttcttcctac tattcaggaa atttagccga agctcctcat gcattaaaaa
     5041 atatcaaaaa attattcatt cacttaaaaa aacagcatga catccaaaaa aacttgcaac
     5101 tgaccattga aagcacgatt cctgctgaac gtggaatggg atcaagcgct gcagtcgcca
     5161 cagcagtcac tcgtgctttt tatgattact tagcatttcc tttgtctcgt gaaatactat
     5221 tagaaaatgt ccagctttcg gaaaaaatcg cccacggtaa tcctagtgga atcgatgcag
     5281 ccgctactag cagcttgcag ccgatttatt ttacaaaagg gcatcctttt gactactttt
     5341 ctttgaacat cgatgctttt ttgattgtcg ctgatacagg aatcaaagga caaacaagag
     5401 aagccgtcaa agatgtcgct cacctctttg aaacccagcc tcatgaaact ggacaaatga
     5461 tccaaaaatt aggatacttg acgaagcaag caaaacaagc aatcattgag aattcaccag
     5521 aaacgttggc acagacaatg gatgaatcac agtcacttct ggaaaagctg acaatcagca
     5581 atgattttct taatctattg atccaaacag caaaagatac cggggcattg ggtgccaaat
     5641 taactggcgg tggacgcggt ggctgtatga ttgcattagc acaaacaaaa acaaaagccc
     5701 aagaaatcag tcaagcactt gaagatgcgg gtgctgctga aacttggata caaggattag
     5761 gagtacatac ctatgtttaa aggcaaagca cgcgcatata ccaacattgc tctaatcaaa
     5821 tattggggta agaaaaatga agaactcatc cttccaatga acaacagcct ttcgttaaca
     5881 ttggatgcat tttatacaga gacagaagtc atcttttctg atagctatat ggtagatgaa
     5941 ttttatctag atggcacctt acaagacgaa aaagcaacaa aaaaagtcag tcagtttctt
     6001 gacctttttc gtaaagaagc cggcctctca ctaaaagctt cagtaatcag tcagaatttc
     6061 gttcctaccg cagctggatt agcctcctct gccagcgggc tagctgcttt agcaggagct
     6121 tgcaatactg ctcttaagct tggattagac gatctctctc tttcaagatt tgctcgacgc
     6181 gggtctggtt cagcttgccg aagtattttc ggtggtttcg ttgaatggga aaaaggccat
     6241 gacgacttaa gttcttacgc taagccagtc ccttccgatt ctttcgaaga cgatttagca
     6301 atggttttcg ttttgatcaa cgaccagaaa aaagaagtgt ccagcagaaa tgggatgcgt
     6361 cgaacagtcg aaacatccag tttttatcaa ggctggttag attccgttga aggggatcta
     6421 tatcaattga aacaagcaat caaaacaaaa gatttccaac ttctcggaga aacgatggaa
     6481 agaaacggac taaaaatgca tggaacgact ttagctgccc agcctccatt cacttactgg
     6541 tctcctgaca gcttaaaagc gatggatgcg gtgcggcaat tgagaaaaaa aggcattcca
     6601 tgctacttta cgatggatgc tggccctaat gtcaaagttt tagtggaaaa cagccattta
     6661 tctgaggtac aagaaacatt taccaaatta tttagcaaag aacaagtaat tactgcccat
     6721 gctggtccag gaattgcgat tattgaataa agggagattt ttatgattga agtatctgca
     6781 ccaggcaaac tctatattgc cggagaatat gcagttgttg aaacaggaca tccagcagtt
     6841 atcgctgcag tcgatcaatt cgtaacagtc actgtagaat ccgcacgaaa agtcggaagt
     6901 atccaatctg cacaatatag tgggatgcct gtacgttgga caagacgcaa cggagaatta
     6961 gttttagata ttcgggaaaa tccttttcat tatatccttg ctgctattcg tttgactgaa
     7021 aagtatgcac aagaaaaaaa cattctttta tccttttatg atctgaaagt aacaagtgag
     7081 ttagacagtt caaatggccg gaaatatgga ttgggttcta gcggtgccgt gaccgttgca
     7141 acggtcaaag ccttgaatgt tttttacgcg ttgaatttat ctcaattgga gattttcaag
     7201 attgcagcac tagccaattt agcagttcaa gataatggtt cctgcggcga catcgctgct
     7261 agctgttatg gtggctggat cgctttctca accttcgacc atccttggct ccaagaacaa
     7321 gaaactcagc attctatcag tgagttactt gccctggatt ggccaggtct atccattgag
     7381 ccattgattg ctcctgaaga tttacgttta ttgattggtt ggacgggtag ccctgcctct
     7441 acttctgatt tggtcgatca agttcaccgt tcgagagaag acaaaatggt ggcttatcag
     7501 cttttcttaa aaaacagtac agaatgtgtc aacgaaatga tcaaagggtt taaagaaaat
     7561 aatgtaacgt tgatccaaca gatgattcga aaaaaccgac aattactgca tgatttatct
     7621 gcaatcactg gggtcgtcat cgaaacgcct gctttgaaca aattgtgtaa tttagctgaa
     7681 cagtatgaag gagccgcaaa atcttctggt gcaggtggtg gcgattgcgg aatcgtaatt
     7741 gttgaccaga aatctggcat ccttccttta atgagtgcat gggaaaaagc agaaatcact
     7801 ccactgccgt tacatgtcta tagcgatcaa agaaaggaaa accgatgaat cgaaaagacg
     7861 aacatgtttc actagctaaa gcctttcatg ataaacaaaa aaacgaattt gacttcgtac
     7921 ggatcatcca taaccctttg ccgcaaatag cagtttctga tgtcgatcta agtacacagg
     7981 cagttgggtt tacactaagc agcccatttt atatcaatgc gatgactggc ggaagcgaaa
     8041 aaacaaaaaa aatcaaccaa gatcttgcaa tcgtcgcaag agaagcagat ttgatgattg
     8101 ccactggttc agtcagcgct gccttgaaag acccttcttt agccgatact tatacgatta
     8161 tgcgacaaga atatcctcac gggaaaatca ttgccaacat cggtgcaggg acttctgtcg
     8221 aaagagccca agaagctatc cgattgtttc acgcagatgc cttacagatt catttgaatg
     8281 cacctcaaga gttggttatg cctgagggag accgtgattt taccaattgg aaagtactta
     8341 ttcaagagac tcaaacagcc attgacgtcc ccctcatcgt aaaagaagtc ggttttggca
     8401 tgacaagaga aacgctcaat gatctagctg ctttaggagt tcatacagtt gatatcagcg
     8461 gtcgaagcgg aacaagcttc acccagatcg aaaacgcccg tcgctccaag cgggaactga
     8521 gttatttagc tgactggggg caatcgacag tctcttcatt gcttgaagca aatgaagcag
     8581 acacttctat ggaaatccta gcctccggag gtatccgcaa tgcctatgac atatttaaag
     8641 ccctttgtct cggcgcaaat gcagtgggta cttcaggaac cgtactgacg catctgatga
     8701 atcacggcgt agaagaaact attatactga tgaagcaatg gcaagaagag cttcgtctgc
     8761 tttatactat ggtcggcgca acgaataccg cggccctaca ccaacaatca ctgatctttt
     8821 ccggaccagt gaaggattgg tgcgaagcaa gagggatcga cttagtgaag tatggaagac
     8881 gcacagaaaa gagcgtggga caaaagtaaa gaacactttt atcccacgct ctaaacacgt
     8941 ataaacggcg gaagcaaaag caactccttc ggaaataagc tgaaattcac aaaaatttga
     9001 aaagcaattt tcgtgaattc cttcttattt cttggagtta aacgcttctg tcccagcctc
     9061 ttttttcgaa taaatgatgt ttaatcagct gtccttcggt attcgttcaa gccgctgatt
     9121 tcacaacctc tccactaaac ggtgtactaa agctctttta aggttgtttt ccgatatagg
     9181 caagtatgcc tccgtccaca tacaagacat ggccgttaac gaaatcagat gcgctagaag
     9241 ccaagaagac agatggtcca gccaaatcga ttggctcgcc ccagcgtgct gctggcgtac
     9301 gtccaacgat aaattgatcg aatggatgac gttcaccgtt ttcttgtgtt tcgcgtaatg
     9361 gagcagtttg cggtgtagca atataacctg gtccaattcc attacattgg atattgtatt
     9421 gaccgtattc agaagcgatg tttttcgtca gcatttttaa tccgcctttt gccgcagcat
     9481 aagcactgac cgtttcgcgt cctaattcac tcatcatgga gcagatattg atgattttac
     9541 cgccgccttt ttcgatcata tcccgaataa ctgcttttga aacgatgaat ggggcattta
     9601 aatccacgtc gattacttgg cggaagtctg ccgcatccat ttcgatcatc ggtgtccgtt
     9661 tgatgatccc tgcattgttg actagaatat caactgaacc tacatcttct ttgatctgtt
     9721 gaaccatcgc ttgaaccgct tcttcatcgg tcacatcgca gacataacct tttgcatcga
     9781 tgcctgcttc tttataatgc gctatacctt catcaactga ttcttgattc aagttattaa
     9841 atacgatttt tgctcctgct gtagctaaag agcgtgcaat ctcaaatcca ataccataga
     9901 cagcaccggt gactaatgct acttttccat ctaaacgaaa catatccata ctaaattcca
     9961 tcattttctc tcccttatgc ttattttaat tgatccattt gcaccatatc catgtctgta
    10021 taggtgatat tttctccgca cattgcccag atgaatgagt aattgcttgt acctacacct
    10081 gaatggatcg accagcttgg cgaaataatg gcttgttcat tgtctacgac taaatgtttt
    10141 gtttcatctg gtttgcccat catatggaac acacgtgtat cttcattggc ataatcaaaa
    10201 tagacataag cttccatacg acgttcatgt gtatgacaag gcatcgtatt ccaagcactt
    10261 cctggttcta aaatcgtata acccatttat aaatgacagc tatcgctgaa gcttctactg
    10321 ctgtaaatat ccccgtcaag ataccaccga taatgatgac aatcaataga aggctaggaa
    10381 tcgcctcttt tattattttt ccagcatgag ccatctgtcc tttttcgaca acagggtatt
    10441 tattcttttt agcaacgatg aatgctacta gcatgattcc tagcgcccac aaa
//
LOCUS       DAFCNS010000086        10323 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFCNS010000086 DAFCNS010000000
VERSION     DAFCNS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.088"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            179..328
                     /locus_tag="LNE23_002381"
     CDS             179..328
                     /locus_tag="LNE23_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBK6932205.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            339..1853
                     /gene="dltA"
                     /locus_tag="LNE23_002382"
     CDS             339..1853
                     /gene="dltA"
                     /locus_tag="LNE23_002382"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBK6932206.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1850..3055
                     /gene="dltB"
                     /locus_tag="LNE23_002383"
     CDS             1850..3055
                     /gene="dltB"
                     /locus_tag="LNE23_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBK6932207.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3100..3333
                     /gene="dltC"
                     /locus_tag="LNE23_002384"
     CDS             3100..3333
                     /gene="dltC"
                     /locus_tag="LNE23_002384"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBK6932208.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3336..4604
                     /gene="dltD"
                     /locus_tag="LNE23_002385"
     CDS             3336..4604
                     /gene="dltD"
                     /locus_tag="LNE23_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBK6932209.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4745..5827
                     /locus_tag="LNE23_002386"
     CDS             4745..5827
                     /locus_tag="LNE23_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBK6932210.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5827..6630
                     /locus_tag="LNE23_002387"
     CDS             5827..6630
                     /locus_tag="LNE23_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK6932211.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6700..7440
                     /locus_tag="LNE23_002388"
     CDS             6700..7440
                     /locus_tag="LNE23_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6932212.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7451..8455
                     /locus_tag="LNE23_002389"
     CDS             7451..8455
                     /locus_tag="LNE23_002389"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBK6932213.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8500..10263)
                     /locus_tag="LNE23_002390"
     CDS             complement(8500..10263)
                     /locus_tag="LNE23_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932214.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3321 a         1703 c         2086 g         3213 t
ORIGIN      
        1 tatatttttt aaaagtacga gaaattataa agaaaaaagc agtgatttga tgtgctattt
       61 ttataaaagc tatgtgacaa aactgttagt aaaaaaacgg tttctgtaag ctagacacat
      121 gtttcttttt gtcaaaatta gttacacttt gtgtgaggtt atatgaggag ggaatccaat
      181 gaacaaatgg cgttctaatc caaaattgaa ttattgggga aaattcatca ttcgaacaat
      241 tttttatagt gcagtactgc tgttcctgat ttatctgtat cactataaaa atattcaagg
      301 aggaactttt atctataacg aattttagga ggaagagtat ggaaatcaaa acgattattg
      361 aagcaatcga tgattgggga ataaatgaac ctgatcgagt tgcttatcag gcggatgaga
      421 cgcatacttt tggtgaattg aaacaagcat cagacgcact tgcttattat ttagaaaaaa
      481 aggtagaagg cgatggacca attgtcgttt ttggcaattt ggagtttgaa atgattgtct
      541 catttctagg ggttgtcaaa tctggccatg cctatttgcc gattgaagag catacaccca
      601 aggagcgtat cctatcgatc tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg
      661 actggataga agatattcct tcggttcctg tcataacaaa aaaagaattt caagaaatca
      721 agaagatacc agtaggattt tatcccagaa atagcgtaaa aggtacagaa aatttttata
      781 ttatttttac ttctggtact actggagaac caaaaggcgt acaaatcagt catgataatc
      841 tagtcagctt tgttcaatgg acgatggaag actttgggat caagcctggt atgcatttct
      901 tagcccaagc gccattttct tttgatttat cggtattttc tatttatcct gcattagttt
      961 ctggcggaat gctgaagccg ttaaataaag cagttgttca agactttcgt caattattcg
     1021 cgacattgcc agccttgaag ttgaatgtgt gggtttctac tccatcattt atggatattt
     1081 gcttgatgga acctacattc aatggagaaa atgtgccgga attggacatg tttttatttt
     1141 gcggagaaga attgacgaaa aaaactgcag aaagtctact tgatcgattc ccaaatgcac
     1201 ggatatataa tacttacggt ccaacagaag caactgtagc tatttcaagt attcagattg
     1261 accagcatgt actagatagt tatgaccgac taccgattgg ctacgtaaaa aaagacacga
     1321 aagtttctat tgtccaagaa ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta
     1381 ttgcaggacc aagcgtatca aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt
     1441 tcttttgtca aaatgactta gcaagttacc atacagggga tgcaggtaga ctagatgaat
     1501 atgggctttt attttatgaa ggacgtatgg atcagcaggt caaattacat ggataccgga
     1561 ttgaactcgg ggatatcgaa cattatctgc tccaagacaa ccgaatcaaa caagctgtcg
     1621 tcgtaccaaa ataccaagga acaaaagtac agcagctggt tgcttttgtc gtattagaaa
     1681 acaaaacgaa acagccggat tttcagttga ctaaatctat caaagaacaa ttactagaag
     1741 ttgtaatgga ttatatgatt cctcagaagt tccattatgt cgatctgctg cctcagacag
     1801 ttaatggaaa aatcgatcga aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc
     1861 tcatatgatc ccttatgcag agccgtttta tttttttctt ttagccattg cgcttgtacc
     1921 gatcgttcta tctttattga tatgggaaaa aagattacca gtttatcaat cagcagtgac
     1981 acttttcttt ttatttgtca gctttggagg taattattgg caacagggag cggcgctgat
     2041 tgggtatgtt ttgtggcaga cgatacttgt atggggctat tttgtttatc ggcaaaggaa
     2101 aaatcagaca ggatggtttt ttgccgccgt tattttagcg ataattccac tgtttttaac
     2161 gaaagtgact cctttttttg aacatggaaa agcatcgtta cttggttttt taggaatctc
     2221 ttatttaacc tttaaatctg tacaaatgat catggaaata agagatggca tgatcaaaac
     2281 atataagcca tttcgttata tccagtttct gctgttcttt ccaacaatct cttctggacc
     2341 aatcgatcgc tataggagat ttgaaaaaga tcagctccat ccgcctgttc gtgataaata
     2401 tgttgatctt ttgggaaaag gcgtccaaaa tatttttgtc ggttttttat ataaattcat
     2461 tatcggttat tattttggtc aagtactgct tcctgtagtg ggacgcattg ccttacagca
     2521 tggtggtatt tcatgggcat tagtaggtta tatgtacgtg tacagtatgt atttgttttt
     2581 tgattttgct ggctatagct tatttgcagt cggtacaagc tacatgatgg gatatcagac
     2641 accaatcaat ttcaataaac cttttttatc atggaatatc aaagaattct ggaatcgttg
     2701 gcatatgacg ctgtcctttt ggttccgtga ttatgtatat atgcgattga tgttcttcat
     2761 gatgaagaaa aaatttttta aaagcaagat cgtaatgtca aatatcggtt attttgcttt
     2821 gtttcttcta atgggtatat ggcatggatt gacgtggtac tacatcgtat atggactgta
     2881 tcatgcttgt ctgatctgtc tgacagatgc atggatccga tttaagaaga agcataaaga
     2941 gcagttgcct tctaacaaat ttacgcatgg cttagccgta tttttgacat ttcaagcagt
     3001 atgttttagc ttcctgattt tttcaggatt tttagataaa ctttttttta gctaacgaat
     3061 agtgacatat taatttatat aacaaaggag aaaaggaaaa tggaagaaca aattttatca
     3121 atcttagaag aaataacagg aacagatgaa gtaagaaaaa atcgtgatat tgatctattt
     3181 gatgaagggt tgcttgattc tttagcatct gttcaattat tagtcgagct agacggagag
     3241 ctggatatcc aagtccctgt atctgaattc gagcgagagg attggagcac accgaataaa
     3301 atcattcaac aagcaactgc attgaagggt tgagtatgaa aaagaaactt tttgctattt
     3361 tcggcccggt tgttttagct gttggcttac tgtttgtgtt ttttacctcc tcctatcaag
     3421 tagatatctc taatccagaa acgattagac gtgcctcttc ttccatgtca gtgaacgtgt
     3481 tgaaaggaga tgcgataaaa aattatgcat tatctgaaaa acaatatatt cctttttttg
     3541 gctcaagtga attaagcaga atcagtccat ttcatccgtc tgttctggca gaaaaatacc
     3601 agcgaaacta tcgtccattt ttgctgggag cacccgggac acaatcgtta agccaatata
     3661 tgatgatgcg ttctgcagga gatgctatga aaaacaagaa agtcgtcttt attatttctc
     3721 ctcagtggtt cgttaaaaat ggtgtaaaaa ctgattactt taatacgtat tattctgaac
     3781 ttcaaacata cgactggttg ttttcaatga agaaagtaac gcctgctgat cgctatttag
     3841 ctagaagatt attaactttc tcaaaagtaa aagagaatga tacgttgacg gcgattctgc
     3901 aaacgatcaa aaaagggaag ctacctttac ctgaatcttt gaatcagctg cgcagccaat
     3961 ggaacatgct gaagagagag gatgaagtat tcagcaatat tgggttgaaa gatcgacagc
     4021 aaaaaatcga tcatgagtca aaacgattac caaaacaata ccaagaaaca gaattatcca
     4081 ttttagcaaa tcaaattgga gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg
     4141 atttttatac gcatcgtatc cgagcccacg agccagagct caaacagtca caaaaaaatt
     4201 gggattatcg tttttcgcca gaattttctg attttcagct ggtcttggat caattagcaa
     4261 aaaaccataa cgaagtattg ttcatcatcc caccggttaa cgagaaatgg agcgattata
     4321 ccggactatc tcaagaaatg ttacaaggat ttgccaaaaa gatcaaattt caattaaata
     4381 gccaaggatt caatcgaata gctgattttg tcaatcaagc gggtaccaac tattttatgg
     4441 aagacacgat ccatctaggc tggaaaggct ggttggctgc agatcaacag atcagaccgt
     4501 ttttagaaga aaatcatatt actgctagta agtatcatct tgacgatgct ttttttagca
     4561 aatcttggca acatcaaata ccagataaac tacaattaaa ataaagaaaa caggcgagtg
     4621 ttgctcgctt gttttcttta tttttgaata ttctgaataa aagataaaaa taccaagttt
     4681 gcctgaacaa aaatcataag caatggtata atcacaatat gaatatagaa atgggggaaa
     4741 acatttggat acaaaagaag aacagctact tattgattta acttctgcaa aaggagtacc
     4801 tggaaatgaa gaagaggtaa gagaagtatt cagagaatat gccaaaccat tcgctgatga
     4861 tattttttat gacggtttag gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa
     4921 ggtctttatt tctggacata tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa
     4981 aggattcttg gaattccaaa ctcttggagg ttggtggggg caagtcatgc ttgctcagca
     5041 agttgaaatc aaaacacgtg aagggaaaat cgtccacggt gtgattggtt ccaaaccacc
     5101 acatgtttta acacctcaag taagaaataa accttatgaa ataaaggata tgttcattga
     5161 tattggcgca tcaagccaag aagaagccaa agagtggggc atacgtcctg gagatatggt
     5221 cactccatat attgaataca aacgaatgaa tggcagtaaa tatttactgg caaaagcatg
     5281 ggataatcgt atcgggacag ctgtttctct gcgagtatta gagaatctat ctaaagaagc
     5341 acacccaaat gttttattcg caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc
     5401 tcgaacaagt acacaccttg taaatcccga tattgctttt gctttagata ctggtacagc
     5461 aggagacact ccaggaatga caccaaaaga agcagattcg attttaggaa aaggcccaca
     5521 gattttgatt tttgatgcat ctatgatccc gcataaaaaa ttattgaatt ttgttattga
     5581 tgtagcagag gaaatggata ttccattcca atatacagtg atttctggcg gaggaactga
     5641 tgcaggacaa atgcatttga caagagatgg tgttccgtct gtagccatta ccgttcctgt
     5701 tcgttatctg cattcccata catcagtgat tcatgaagat gattatttga atacagtgaa
     5761 actagtcaca gaattagtga aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta
     5821 tgaataatga ataatagaat acctatttct gaatatcgga aaaaagcacg agtttctttg
     5881 gaaaatcaat ggggaatcaa tacttggctt gttttcttat ctataatatt tacgagcgtc
     5941 atccagaact tttttgacgg tttcagtcct tttccggaag aaagcatgca gcagcactta
     6001 ttgagttttc tagtcgataa tttactttta ttcgcattca actatgcatt gttttacatt
     6061 gcgctagtag ttgtacgggg tggcagacca aaaagtaacc ttttattttc agttttccaa
     6121 agaaagtatt ttggttcgat acttggcatc aatttgatca attcagtcat caactggcta
     6181 ctcaacagtt taatctttct gcccatgttt ttaatcagcg gagtgagtac ttatatgcag
     6241 ctgcttttca gttcaaatgc ggtacctacg ggtgtcatgc cagaagattt tcttgatatc
     6301 agctttgttt tatcgatgat agggatgatt tttgtttcag cactcatcat gagtattgtt
     6361 ggcggtttat tccaatttgc tgcatggaca aagttcgact atcctgattt gtcggttatt
     6421 caatgtttga aatatggctg gtttttattg aaagatagaa ttggtacgta tattttgttg
     6481 cagctgtctt ttatcggctg gtatttgcta ggagcaatcg ctttgttcat cggtcttttg
     6541 tgggtcattg tttatgtgaa tgtgtcagtt gcagaatttt atgaacaagc acggattgaa
     6601 aaaggaggtc cagacgccta ttttacttag catgtaagca gtttattcat caaaaaaata
     6661 acttttgtgg taggataaat ctttgagggg gaatgtacga tgaaattaac tgttttagga
     6721 tgcttaggag cgtatcctta taaaaatcaa gggacaacag gttatttgct tcaatctgac
     6781 gggttcaatt tgttgatcga tgcaggtagt gctacgctga tcaaattaca agaacacctt
     6841 gatccacttg atctagatgc tgtgatcctt agccattatc atcatgatca tattgctgac
     6901 cttggtgtat tacagtatta ctggcaactt catcctaaga gaaatccgga gacattgctt
     6961 tcaatttatg gacacacaaa agattccatt cactttgaag aattgacgtt ggaaaatgtc
     7021 tcaaaaggac atccttattt tgaagctgaa gaattaaaaa ttggtccttt tctgattact
     7081 tttatggaaa caatccatcc agtagtatgt tacgccatgc gtatcgtaga agaaacaacg
     7141 gggaaagtat tcgtatttac aggtgattca ggttatttgg aaagtttcgc tgattttgcg
     7201 aaagatgctg atttgtttct agcagatacc tatctttttg aaggaaatga acatcatcat
     7261 gcccatttca cttcaaaaga agctggagaa cttgcgaaat cagctaacgt aaagaaactt
     7321 gtacttacac acctacctca gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag
     7381 gcagcaggca aagggattga tatagaactt gctgcagttg acaaagtgtt taacatttag
     7441 gaggaagaac atgatttttg taccaaatga aaaccacgac ccaagaatca atttagcaat
     7501 tgaacaatat ttgctgcaag aaatgccttt agatgaacct attttgctct tttatatcaa
     7561 tgaaccttca atcatcatcg gcagaaacca aaatacgctt gaagaaatca atcgagatta
     7621 tgtggaagaa aatggcattc atgttgtccg tcgcttaagt ggaggcggag ctgtctacca
     7681 tgattttgga aacctgaact ttagttttat catgcctgat gatggggatt cattccgtga
     7741 ctttgcgaaa gtaacacagc caatcatcca agcacttcat gaattgggtg ttgctggggc
     7801 ggaattgaaa ggccgaaatg atctagtcat tgatggcatg aaattttctg gcaacgcgat
     7861 gtatgctacc aacggtcgga tgtttgctca tggaacaatt atgttcgact ctgatatcaa
     7921 tgaagtagtc aatgcattaa aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc
     7981 gatccgttct cgtgtaacga atatcaaacc atttcttcca gaagaaaagc aaaaaatgac
     8041 aacagaagaa tttcgtcaag aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga
     8101 agtaaaaacg tacgaattga ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga
     8161 atattatcga aattgggatt ggaattacgg gaagtctcca gatttcaatt tctctcgcca
     8221 aaaacgtttt tctatcggtt cgattgaagt ccatttgaat gtctcagaag gaatgatcaa
     8281 agaagcaaaa atctttggcg atttcttcgg actaggagat atctctgacg tggaaaaaaa
     8341 attggttgga cagaaatatg ataaagcatc tctatcaaaa atagtcagcc aaattgatgt
     8401 gaagaaatat tttggtgcga tagaaccaga agaattgctt accttacttt attgaaaata
     8461 aaaaatagcg gaatccacat atatggattc cgctattttt tatacaggat tcgggtaatt
     8521 tcgagagagt tgaacagtga tccgatcaat gaaagtatct ttttcctgcg ttagtgttcc
     8581 tgtgtaacga tccgctacgt ctttcatcat cattaagtca aaacgttcct gatcatctaa
     8641 ttcttctgtg atgcctcgac taaataggtt tgtatcgtaa tgtgcactat gttcgcaaca
     8701 aatgacgaaa tgttcctctt gttcgtgtat cgacaaataa atttttcgat acagtggatt
     8761 ttcgatggca tgagtttctc taaaggaatg ctctaaagcg tggatcaaaa gttgagccag
     8821 atcgtcatcg ttgataggaa gttcttcggg aagatctgct tggaaggtta tctgaatatt
     8881 ccgtttggca gctaacttct ggtaacgcgc taagataaga tttgtcacct ggtggttgca
     8941 aaaagaacac agcatttcga aattttttgc ttcttctaat atcttttcca catagttttt
     9001 agcttttgtt tggtccgtat gcattaaatc ttgcagattc tccatctctt ggacaaattc
     9061 caggcgaagt gtatttactt cttggatatg ctgtcgaagc tgctcataat gttgctccaa
     9121 caaatcattt ttgattttta agaaatctac ttgtctccga tctttgtcgg tatttaagat
     9181 gtgatcgatt agattgtagc tgataataac gattagaatg ataatatata aaatcgtaga
     9241 ccaattgatc gttgtttgat agatacctaa tgccgtttgg atgtaaatca agcaatggat
     9301 aatggctacc aagacgatcc aaggtgtaca agctacataa aaacggttat tcttatatgc
     9361 ttcgccaatg catgcaagtg aagtgactaa agtgcttata acactagcaa tattagcaat
     9421 cggcattaat tcaggaagtt ctgcaggagt aaaagttgca taaagcagaa cgccgataaa
     9481 aatcaaaagt tggaacgtac aaaaaattcc gtaccatttt ttatagtaaa acattcggct
     9541 ccaatagtag cagatgatga aagctgacgt taagataata aataaatgag ataaaaaaga
     9601 atgaacgaat ggttcaaaaa gaataccact taaaatatct tctgatacag cttcaaatcc
     9661 taatgccaaa gcaaagcaac ttaagagaat aagtgagtat tcaagacgtt tttgtttata
     9721 catcaagtag atcgtataga aaaaggtccc tatagcgata aaaacagcga ttatcatcgt
     9781 caatacttgc ggcactgatt gtgaataaat aaaactaatc aatggtccag ttgttcctag
     9841 ataaactttc ggtggcagtc cagcatagtt ctgataagga gatgagactg taagggtcaa
     9901 tttttttccg atataatttg ctggtaaatc tatcacagcc aatgagagtc cgggattgct
     9961 ttcttcctga taatctttcc gttggtataa aatcttttcg cctagatata cagtaatcca
    10021 ttgatggttt cctttgatta gtaaatttgc ttcgtccaac ggtttttcaa gtgtcgtttc
    10081 cataatgaat gtttcgtctt tatccacggt aggaatcgta cgagtgtact tgctgtcaaa
    10141 cttaaaatca ggatcatctt ttgtatagaa aacccacgaa tcttttattt ctttcatttg
    10201 attgctgtcc gcaggagaaa aaataaaata aaagaaataa gcaattcctg ttaaaaataa
    10261 caatgataat ataatgatta tgctccactt tatacgtaaa taaaaagatt cttttttatt
    10321 ata
//
LOCUS       DAFCNS010000087        10236 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFCNS010000087 DAFCNS010000000
VERSION     DAFCNS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.089"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            311..1090
                     /locus_tag="LNE23_002391"
     CDS             311..1090
                     /locus_tag="LNE23_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932215.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1136..2080
                     /locus_tag="LNE23_002392"
     CDS             1136..2080
                     /locus_tag="LNE23_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6932216.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2096..2875
                     /locus_tag="LNE23_002393"
     CDS             2096..2875
                     /locus_tag="LNE23_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBK6932217.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2887..3585
                     /locus_tag="LNE23_002394"
     CDS             2887..3585
                     /locus_tag="LNE23_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBK6932218.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3613..4377
                     /locus_tag="LNE23_002395"
     CDS             3613..4377
                     /locus_tag="LNE23_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBK6932219.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4421..5872
                     /locus_tag="LNE23_002396"
     CDS             4421..5872
                     /locus_tag="LNE23_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK6932220.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5904..7040
                     /locus_tag="LNE23_002397"
     CDS             5904..7040
                     /locus_tag="LNE23_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6932221.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7040..8146
                     /locus_tag="LNE23_002398"
     CDS             7040..8146
                     /locus_tag="LNE23_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBK6932222.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            8152..9174
                     /locus_tag="LNE23_002399"
     CDS             8152..9174
                     /locus_tag="LNE23_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HBK6932223.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9196..>10236
                     /locus_tag="LNE23_002400"
     CDS             9196..>10236
                     /locus_tag="LNE23_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6932224.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKL"
BASE COUNT         3741 a         1441 c         2100 g         2954 t
ORIGIN      
        1 tattattaat tcaatgttaa tttttttgtt attctatttt ttgtgtaact tttaaaaaca
       61 cctatgaaaa ttatggaaat aaaaatgaaa tcgattaatt tttatcttat taatgtttta
      121 tttactttta aatcgtaaat tgccctagtc aagaaaacag tttgtggtat gattatatag
      181 ggaaaacttg tttacaaata tttctgtgaa tatattagtt taattatgtt tttttaaata
      241 aaaagctgtc atttttatgt aagtgaatta tatcttaatt tatctatcat caatacttag
      301 gagtgaatct atggaaaagc cattcgtcaa gtcattgact gctgtggcgg taggtgcagg
      361 tgctattgcg atctgtttgt ttggatacca tatcaataat cagcggcaac atcagcaacg
      421 gatcaattat gcagaatctg ccatcaccaa tcaaaaagac accttgactt ctttatcaaa
      481 agaagtagac aagctgtata gtacaaaaga aaaaattttt ctaaaccctg aaataacaga
      541 agaaacaatc acaagtttgt caaataagct atcctctgtc aaactttcag cagatgatta
      601 cgacatcaaa gaatcagaat tgcctaaaga agctgttgca atccaagatg agaagaaagc
      661 aatcgtgaca cagttagata gtgcagaatc aaaactgaag atccagacag ctgtcaacga
      721 tttatttact aaaaatgtat caaactggca gcaagctgta gacgatgtca tcatcaaaga
      781 aaaggtgaca agtgcagatg tcgctcgtgt acgtgaaaat atgagctttt ttaaagatag
      841 tccttggaaa acggttgtta tgcaatatct aggattcgct gatattcaga tcgcgcaagt
      901 cacgcaacta gatcagttat tcgatacgat gttaaaggat ggtcaagtga ctgccaccgc
      961 tacttacgat caatatttga cggtgctcag tcaaatagag cagatccgga atgaaaaaat
     1021 aagcgcagca tacgcaacaa aagcacaaac tgttgctgag caaatgggct atagcaatat
     1081 aagctattga cagttaaaac gttgattcaa gcaaggttag caaacggagg aaactatggc
     1141 aagacgaaga agaaaatcaa gagggaaaaa aatatttctg attattttag cagttcttat
     1201 cgtattagta ttagcaattg taggagtagg agcaaaatta tatctggatc tctcacattc
     1261 tattgagaaa acttacgaat cagtggaacg aacccaagga gaacgaacga aaccagttga
     1321 tctggacaaa caagaatctt tctctgttct attgatgggg attgatactg gagatttagg
     1381 tcgggtcgat caaggaagat cagatacgat gatggttgcc acagtcagtc ctgaagacaa
     1441 acagacaacg atcgtcagca ttcctcgtga tacgtatgtc gacatcgtag gtcgtgacac
     1501 aaaagacaag atcaaccacg cttacgcatt cggcggagct gccatggcga tggataccgt
     1561 acaaaaatat gtagacatcc caatcgatca ctatgtctct atcaatatga aagggctaaa
     1621 ggaactcgtt gatgcagttg gtggtatcga agtcaataat gacctcactt tctcacaaga
     1681 tggctatgac tttccaatcg gtaaaattac attgaacggg gatcaggcat tatcttattc
     1741 tcgtatgcgt tatgaagacc caaatggtga ttacggtaga caagggcgcc aaagaaagat
     1801 cgtagaaggc gtagcgaaaa aagtattgag tctaaacggt gtcagcaact atcaatccgt
     1861 tttgaatgct ctagagtcga atatgaaaac agacatgaac tttgatgaaa tgaaaaaaat
     1921 tgctttagat tatcgggagg catttggtac gattaagcaa gatcagctgc aaggagaagg
     1981 cttcatgcaa gataatatct cctatcaaaa agtcagtgat caagaacttg atcgtgtgaa
     2041 aaaagaatta aaagatcaaa tgaatctaga gaataaatag gaaagtggag aaaaaatgga
     2101 agagacaatc agcttgaaag aaatttttga aacattacgc aaacacttgg taataatcat
     2161 catctcaatg tttgctggct tggccatctc aggaatcgta acatttttca tcttgacacc
     2221 aaaatacagt tcacaagcac aattgatcgt gacactgcca caatcagaca ctgcaaatgt
     2281 caatgatgtc aatacgaacc tgcaaatgat caatacgtat aaagatatga tcgtcagtga
     2341 tttagtgctc aatgaagtca aagatcgttt agaaacagaa gacaatgtga aaatgactgc
     2401 tgaacaaatc aaagatgcca tctcagttaa tcaggcagaa aattcacaaa tgttctcgat
     2461 tcaggcaata agtacgaatg cagtggctgc tcaacagatc gctaacacaa cttcacaagt
     2521 tttccaagag aacgcaaaag atgtgatgaa tgttgataaa atctcgatca tttcaggtgc
     2581 agttgccaat acgacacctg tttcgccaaa taataaattg aatttaatga ctggcttaat
     2641 gttaggaatg atggtaggtg taggtctggc attcttattg gaattactcg atcggacagt
     2701 gaaagatgaa aaatatgtga cagatacatt aggattccca atcttaggaa cagttccaga
     2761 aatgtcagcg aaagaattga atgctggtat ccgtaaaaaa ggaccagctc agacacaacc
     2821 aaaagctaca aacgataaaa cagcgccatc acgcagaagc cgctcaagag tctaggagga
     2881 aagaaaatgg cacgaacaca gaaacaaaaa gcagcccaaa ctcatgcagt cagtttggtc
     2941 acactcacca atccctcttc accaatcgct gaacaatatc gtacgattcg gacgaatatc
     3001 cagtttgcct ccgcagctgg tcagcaaatc aaaacgattg ttgtgacttc atcaggacct
     3061 ggagaaggga aatcgaccac tgcagcaaac attgccgttg tttttgccaa atctggtcaa
     3121 cgggtgctgc tagtcgatgc agacttacgt aagcccgtca tctataaaac gttcaagttg
     3181 aacaacgcaa gcggattaag tactgttctt agttcatcag gaagtgtagc agatgagatc
     3241 caacgaacac cagtcgacaa tttgtcgatt ctgccaagtg gacctaaacc accaaatcca
     3301 tcagaactac taagttcgcc aagaatggat cagattttag cagaagcaag acaactattt
     3361 gatgtagtga tcttcgacat gccgccagtc gttgccgtaa cagatgcaca gatcatgtct
     3421 tcaaaaacgg atggcacctt attggtcgta cgggaaaata cgtcacgaaa agaatcattg
     3481 actaaagcaa aagaactgtt agatatggta caagcacgag tcttaggtgt cgtatacaac
     3541 ggagcagaac acagcaaaga tgctggttat tactacggaa attaaggaat aagaagagag
     3601 gagacaagag aaatgatcga tctacattgc catatcctac caggaatcga tgacggggca
     3661 gaaaacttag aagcaagtat tgcaatggct gaaaaagcga ttcagcaagg cattacgcat
     3721 attttatgca caccacacca caataatgga aaatacagca atgaaaaatc acaagtcatt
     3781 tctctagtcg cttcgttaca agccgaatta gagaaaagac agttgccttt gacgttattg
     3841 gaaggacaag aagtacgtat taccggaacg ctcatcgaag acatccaccg agatgaaatt
     3901 ttatttacag atttagatga tacatatctt ctgatcgaat ttccgacact ggaagtgccg
     3961 ttatatgcag aacggctttt ttttgagttg ttagaactag gaaagatacc agtgatcgtc
     4021 catccagaac gcaacgcgca ttttcgagaa gacccaaatc atttgcttcc gttcctagat
     4081 atgggatgtt tggcccaact tactgcgcca agttatgttg gtgtatttgg caaagatatc
     4141 cagaaaacag ccaaaacaat ggtcaaacat aatctcgtgc agatggctgc ttctgatgca
     4201 cacggggtct ctaagcgcac cttttactta aaagagtgct atgagcagat tgccaaggag
     4261 tttggtaagg aaaaagtagt acagatgaaa caagtcgcaa aggatctgat caacggggat
     4321 gagatcaatt atccaatcta tgaagaggta aaaaagaaga agtttggttt attttagttc
     4381 tttattcgta gataaaaagg aaatgaggag agagtgagag atgtatagaa aaaactcaag
     4441 tggttggtct aaacatattg attttatttt attagactta ttttgtgtcc agcttgcttt
     4501 ttatatatca tatgtaatgc gacagggtga ctggaatccg tatgtgattc ctatttatcg
     4561 ccacatggca atttttatag aacttgagaa tcttacgatt atatttttat ttgaggcata
     4621 taaaaatgtt ttaaaaagag gatattataa agaggctgta gcttctataa agcaatcttt
     4681 tatgctgtta cttgtgtgca gtttatacct tattacaatg caggatggaa atgattattc
     4741 cagagtttct ttatttataa tgggggtggt gtatggatta ttaacatata taattcgtat
     4801 actttggaaa gggttgttgc atcaccgcat gaaaaacggt gaagatgtat cgttagttat
     4861 tgtaacatct caaaagattg caaaaaatgt agttcgtaat cttaaagaga agaattatgg
     4921 catgtataaa cttagtggtg tgattgtagt agacagagat atgtatggaa ctaccatcgg
     4981 aggtgtccca gtagttgcaa atgctgataa tgcagcggaa tatcttttac agaattgggt
     5041 agatgaagta ttcatcaatg ttgacgaaag tgttccatat cccaaagaat taatcgccag
     5101 atgttcggaa atgggacttg ctgtacatat gaatcttaca aaagtgtctg atagtacacg
     5161 tggaaaacaa tttattggcc gagttggtga ttatactgtt attacatcta gtattaatgt
     5221 tatgacgatg aggcaagcat ttataaagag aacgattgac attcttgctg gaattattgg
     5281 atgtattgca acaggtgtta tttttatctt cgtagcacca gttatttaca tcaaatcacc
     5341 agggccgatt ttctttgcac aagaacgtat tggtcaaaat ggtaagacat ttaagatgta
     5401 taagttccgt agtatgtata tggatgcaga agaacgcaaa gcagagttga tgaaagaaaa
     5461 taagatgagt aataatctca tgtttaagat ggattttgac ccaagaatca ttggcaataa
     5521 aatacttcct gatggaacaa agaagacagg aattggacaa tttattcgat ctacatcatt
     5581 ggatgagttt ccacaatttt ataatgtcct aatgggaaaa atgagcctgg tggggtttag
     5641 accttgcttg aagtcagaat atgagaagta tgattttcat catcgtgcga gaattgcgat
     5701 gaaacctggg attactggaa tgtggcaggt gagtggtaga agtgatatca cagacttcga
     5761 agaggttgta aaacttgatg cagaatatat caggaattgg agtatgggat tagattttag
     5821 aattttgttt aggacagtca aaactgtgct tcatagaaac ggttccatgt aagataaaga
     5881 tactatagaa ggagtaaaac gtgatggcaa gaaaaaaatt aaaaatctgt atgctaggtc
     5941 ataaaagaat tccatcaaga gaaggtggaa ttgagatagt ggtagaagaa ttggcaacgc
     6001 gaatggttgc acttgggcat gaagtgactt gctataatcg aagtggtcat catgttagcg
     6061 gaaaggaatt tgaccagcat aaaaataaag aatataaagg tgtaaaatta aagactattt
     6121 ttacattaaa tataaaagga attgcagcaa tgagttcttc agtatttggt ggaattagag
     6181 cagcattcgg aaaatatgat attgttcatt ttcatgcaga aggaccatgt gctatgttgt
     6241 ggttgccaaa actttttggg aaaagatgtg ttgcaacaat tcatggtcta gatcatcaaa
     6301 gagaaaaatg gaacaagctt gctagtacat acatcatgtt aggggaaaag tgtgctgtaa
     6361 aatttgcaga tgaaataatt gtacttagtg aaagcgtcca aaattatttt gaagatatat
     6421 atggaagaaa aacaaggttt ataccaaatg gcgttaaaaa aattgaaata aaatcagcag
     6481 gattgattac agaaaagtat ggattaacaa aagattctta tattttgttt ttggggcgat
     6541 tggttcccga aaaaggaatt cggtatctaa ttgaggcatt taaagatgtt cagacagata
     6601 aaaaacttat aatagccgga ggaagctctg atacagatga atttgcaaat gaactaaagg
     6661 aattagcaaa aggtgatgag cgaattatat ttactggatt tgtacaagga caggaattag
     6721 aagaattata tagtaatgcg tatatctata cacttccaag tgatttagaa ggaatgccat
     6781 taagtttgtt agaagcaatg agttatggga actgttgtat agtttcaaat atctcagagt
     6841 gtacggaagt agtagaagac aaggctatga tttttaagaa atcggatgta agtgatttaa
     6901 agataagatt agaagaggca tgcaatcaat cggaaatggt taaagttttg aagaatcaag
     6961 ctactgagtt tatttgtagt aaatataatt gggataaaat cgtacaagaa acattaaatt
     7021 tgtatagggg aaatatataa tgattcgaat attacagtgt gtgaatgata tgcatcgtgc
     7081 agggttggag acgatgctaa tgaactatta taggaatata gatcgaacta aaattcagtt
     7141 tgattttttg acacatcgtc catataaaag tgattatgat gaggaaataa taagtcttgg
     7201 aggaaaggtc tattatgcac cgagacttta tccacaaaat tatcctaaat attttaaatg
     7261 gatggaacaa ttttttaagg atcatccaga atatgagatt gttcattctc atattgatgc
     7321 gatgagttat ttacctttaa aagctgcaaa aaaagctggg attccgatac ggatagcaca
     7381 tagccataat acatcgatag atagagattt taaatatctt ttaaagcaat attatcgatg
     7441 taagttaacg tctgttttga cacatgaatt ttcatgtgga aaagtagcag gtgatttttt
     7501 gtttcgaaat gataaattca ctttgattcc aaatgcagtg gatgctaaaa aattttatta
     7561 tgatgaaagt attagaaatg atgtacgaat gaaattagga atttcaagtc agacgtttgt
     7621 aatgggacat attggaagaa tttcatattc taaaaatcat cgttttttga ttgaaatttt
     7681 taaagaattt cacgctatta agaataattc cgtattatta attattggaa ctggtgatat
     7741 ggaagaagaa ataaaaaatt atgcaaagaa aagtggcata gatgatgata ttaaattttt
     7801 aggaaataga aatgatatag aaaaatttta tcaagcattt gacgtattaa tgttgccatc
     7861 attatttgaa ggggttccgt tagtgggtat tgaagctcaa tttgcagatt taccgtgttt
     7921 cttttcggag aaagttccta cagaagttgc tttcagtgat aagaccaatt tcataagttt
     7981 aaatcaatcg gcaaaagaat gggcgagaga gattgcaaat gtagatatct ctcatcgaga
     8041 ttcagaacgt tcgattttaa taaaggcaga ttataatatt caaacagcat acaaaatctt
     8101 agaatcaaaa tattatgagt tatttgaatt aatacaaaga ggttaaagaa tatgacacag
     8161 aaacattcaa gtataaaaag aattatacat cgcatatccg aagaattgaa tgcaacaaga
     8221 cgaattgcaa agataacatc ttggaaggaa gcatttgcca cgttacaagc taaaattgat
     8281 attcaagtca tgaatcgtaa tggatataat gaaagtaaaa aacagaagga acatttatta
     8341 aaaaaacatg aggtaatgct gaagtattat gaaaagactt tttcagattt tttgaaaaca
     8401 tatgattata gtcaaaatca aataattaat caatcaaaat caccatattc taactgcatt
     8461 tgggtttgtt ggtggcaggg attggatcaa tcgccggaaa ttgtaaaagc ttgtgtaaaa
     8521 tcaataaaac gaaatgcagg gaaacatact gttataattt taacagaaga taattataga
     8581 caatatgttg atataccaaa atgggtggaa gaaaagaaaa acaaaggaat cattacgaga
     8641 acaaattatt ctgatttatt aagattatct ttgctagcaa aacatggagg tttgtggtta
     8701 gacgccacat tttattgtac aaaaccaata ccagatgagt attttgatat cgatatttgg
     8761 tctattagga ggcctgatta tgctcacgca tccgtagcat caggatattt tgcgggttat
     8821 tcattagcat gtaatcaaga gtctagatgg atgtttgcta taattagaga ctttttttta
     8881 aattattgga aaaataatga tatgatggtg gattatttga tgatagatta tatgattgtt
     8941 ttagcacaga gaataaatca tgaaatagca cagacatttg ataaaatacc tagtaacaat
     9001 ccattatgtg acgaattgat taaaaattta aataatatat atgatgaaaa agtatggaac
     9061 ttattaaaga aagatacttt actgtttaag ttgacatgga aacaatcttt taaaaagcaa
     9121 aaagatgata aagttacttt ttatgggatg ttggtagaaa acaaattatt ataaatatta
     9181 gaggtataaa aagttatgaa acacaaggta agtgtgatag taccaattta caatgttgaa
     9241 aaatatttaa gaaaatgtgt ggatagttta ttgagtcaga cattaaaaga gatagaaatc
     9301 gtattagttg atgatggttc gccagatgct tcgggggaaa tagcagatga gtatcaaaaa
     9361 aaatattcta atgtaaaaac aatacataga gaaaatgggg ggttaggacc ggcaagaaat
     9421 acaggaattg agaatgcgac aggagaatat gtggcttttc ttgattcaga tgattgggta
     9481 gaaagtgata tgtatgaaaa attatactta gttgcgtcca aagaaaatgc tgatatagta
     9541 gttagtggac attgtgattt tgcgaatgac aaagctatgg tgaaaaagca acatcctttg
     9601 agtgggaatg tatatgatac gcctaatagt attaaaaatg tacgaaaaaa tttatttgga
     9661 catagtccaa aagatcaaga agtagaggcc ttcccgatgt cagtatgtat gtctttgtat
     9721 aataaaaatc tgctagataa atatagacta agatttgaaa aaattttatc tgaagataca
     9781 atttttaact tgaatgcata tgatgtcgca aataaaattg cttttacaga atatacagat
     9841 tattgttata gaaaagaaga acaagagtct attacaaaaa gtttatctcc aaataagcag
     9901 acggaattta aagaattttt aataactcta aaagctatag catcgaaaga agatttagaa
     9961 tgtcaaatca gagcaaaacg tatggcaatt gattattgta ggctttatgt gggcttagtt
    10021 gatgatgcaa atttgggaat taaagagaaa agaaagtata taaaaaaatt tattgaagat
    10081 gagggattaa ttcaatgttg gaagggatat cctacaaata cgctaccatt tcaacaaaaa
    10141 ttttttcata aaatgcttga atgtaagtgt tatacaatat tgttgttgtt aagtagaatt
    10201 agaaagataa ttaaaaatat gaccagaaaa aaattg
//
LOCUS       DAFCNS010000088         9888 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFCNS010000088 DAFCNS010000000
VERSION     DAFCNS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.090"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(109..2229)
                     /locus_tag="LNE23_002401"
     CDS             complement(109..2229)
                     /locus_tag="LNE23_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK6932225.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="LNE23_002402"
     CDS             2459..3637
                     /locus_tag="LNE23_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBK6932226.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="LNE23_002403"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="LNE23_002403"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBK6932227.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="LNE23_002404"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="LNE23_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBK6932228.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADMK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="LNE23_002405"
     CDS             complement(4990..6243)
                     /locus_tag="LNE23_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6932229.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="LNE23_002406"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="LNE23_002406"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBK6932230.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="LNE23_002407"
     CDS             7821..9167
                     /locus_tag="LNE23_002407"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBK6932231.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="LNE23_002408"
     CDS             complement(9475..9861)
                     /locus_tag="LNE23_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6932232.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3148 a         1800 c         2026 g         2914 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatatg aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttatta
//
LOCUS       DAFCNS010000089         9546 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFCNS010000089 DAFCNS010000000
VERSION     DAFCNS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.091"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            202..447
                     /locus_tag="LNE23_002409"
     CDS             202..447
                     /locus_tag="LNE23_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932233.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            complement(631..1380)
                     /locus_tag="LNE23_002410"
     CDS             complement(631..1380)
                     /locus_tag="LNE23_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6932234.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1566..2270
                     /locus_tag="LNE23_002411"
     CDS             1566..2270
                     /locus_tag="LNE23_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBK6932235.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            2310..2867
                     /locus_tag="LNE23_002412"
     CDS             2310..2867
                     /locus_tag="LNE23_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6932236.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2875..4014
                     /locus_tag="LNE23_002413"
     CDS             2875..4014
                     /locus_tag="LNE23_002413"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBK6932237.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            4026..4457
                     /locus_tag="LNE23_002414"
     CDS             4026..4457
                     /locus_tag="LNE23_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6932238.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            4471..5916
                     /locus_tag="LNE23_002415"
     CDS             4471..5916
                     /locus_tag="LNE23_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBK6932239.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            6202..7827
                     /locus_tag="LNE23_002416"
     CDS             6202..7827
                     /locus_tag="LNE23_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK6932240.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
     gene            8459..8812
                     /locus_tag="LNE23_002417"
     CDS             8459..8812
                     /locus_tag="LNE23_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932241.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
BASE COUNT         3326 a         1327 c         1833 g         3060 t
ORIGIN      
        1 ttttctattt aatcacattt gattctaaaa aagaaacaaa aataaaagtc atgtatatgg
       61 tggtcaaaaa gtaaataata tcagttatca aatttcctgt aaattttctt gaaactacag
      121 catattatta cttcaaaaca atctacattt tttataaaaa aggaaatgaa taactgaaaa
      181 aattattata ggagagaaaa aatgtcaaaa tctaaaaaaa atatcaccat aattggttta
      241 ggcttaaccg tatatttatt tacagaattt gtttatagac atgtgaattt atttgcagaa
      301 cctttatttg tattatacga atcaaattcc tcaatcgtca gtgaacttgc taatttaatg
      361 atagcattgc tgtttactcc cgcgttgaga gtcataacta ttttggtctg tttagtgcta
      421 ttgttttttg ttaataaaaa gaaataacgt agtatttcaa aagacaaaga agtgggatga
      481 tgattgctat agtatgttat acgtggtgct aggtaagtga gaataaacaa aaaaggaaac
      541 acgcaataaa gtttatttca aaactttatt gcgtgtttct tttttagatt ttttatatct
      601 gttgctttag tagttaccaa tagttttcgc ttaatcaatc aaaacgattt tttgtttatt
      661 gtattcttta cgaacctttt caggaatttc ttctgaaata agataggtaa ggtcttcaat
      721 tggaccgacg atatgattac tgatggtgcc gaatttatca ttagttatca tcccaagtac
      781 ttcagtagaa atacgtatca tctcacgttt aatgtcggat tcttgcatag ttggcacgct
      841 cgttccaaca tctagatcta tgttataaat acccataata taaaggtcgg ctcgaatgtt
      901 ttgtagttgc ttataagctt gtaccccaat attgatcata gattgtttat aaaaagtacc
      961 gccaatattg ataatttcca aattaggatg ctgtgctaat gctatggcaa tcggcgggct
     1021 attggtaatg attgttgcac gaaaatcttg tgggatttct tttgctaaag ccaagttagt
     1081 tgtacttcca tcaatgatga tgacagattc ttcttttaaa aaagggaggg ctttatgagc
     1141 taatgcagtt ttttgttcgc tagctacagt ttctcggtag ttaaaattgg taattggcgg
     1201 accaacacgt aaagcgccgc tgtgtactct ttttaagaga ccttggctat ccaaatcttt
     1261 caaatctcgt cgaatcgtgt cttcggatac attgaaacgt tgacttaaat cactagcgat
     1321 aactttttgt tctgcgttta ataaatctag aattttttga tgacgttcta atttcaacat
     1381 gttgcgtgct ccttcttatt tattcttgtt tatcatgtca tttatgcgtt tttatgtcaa
     1441 taatttccgt ttttaataca ctaaaaaggc ataaacaagc aaaaacaaaa tatttttatg
     1501 cagttttatg ttgactagta tttgttaagc ggttacaatg agggggtaat taaaggaggg
     1561 aatgtatgga gaaaaaaatt attttgtcgc cttcacttat gtgtggggat ctagcaaatt
     1621 tggaaggaag catccaagaa ctagaaagta ttgggatgga ttccttgcat attgacgtga
     1681 ttgacggttc ttttagtccc agtatgcctt tagggatcga aacaatcaaa agaattagag
     1741 aaatcacttc aatgaatttt gatgtacaca tcatggctaa ggataacgat aacgaatttt
     1801 ttattggaga aatgttgaaa attggcgtag agagcattac ttttcattat gaaacaagcc
     1861 tccatattga tcgatatatc cagctcatca agaattctgg aacaaaagta gggatagctt
     1921 tgaatccagc aactccgtta aatgtattac aatatatttt acccgagatt gataggattt
     1981 gtttgatgct gattaatcca ggttttgcaa caaacaagtc agaaaaacaa gtttcttacg
     2041 cgctaaaaaa aattacagat ctttgccagt tagtaaatga gaaagagtta gcagtagata
     2101 tccaagtgga tggacgggta tcgttagaaa cgatcccggg attattagat gcaggcgcaa
     2161 caaatctggt attaggaagt acaagtttgt ttcttaaagg aaatacatta gcagaaaatc
     2221 aaaaactact ttatcaagcg atgaagcaat cagttaggaa aaatcaatag atgtagtggt
     2281 cctgaataat agaaatggag gggaaatagt tgagttataa ggcggcgaaa caaaatattg
     2341 taagagaaat tactgaaaca ttagaccgta tttcttcaaa agatgtggaa caattgataa
     2401 agaaaattga aaaagcggaa cgagtttttt tcgtgggggt tggcagagta ctgttagcgc
     2461 tagaaggaat ggcaaaacgc ttagggcatc taggaattca gacagtagtc gtaggacaaa
     2521 tcactgaacc ggcaattaca gataaagatt tattgattgt tggttctggt agtggggaaa
     2581 gtgctttccc actaactatt gcaaaaaaag caaaggaatt tgggtcccaa gttgcccata
     2641 taggatcaaa cccagacagc acaatgaaag aatattcaga tctttttatt cgtatacccg
     2701 tacaaacaaa attagcgtta ccagaggaaa tttcttctat acagccaatg actagcctgt
     2761 ttgaacaaag tgttcttttg ttaggagata gtattgcttt agtgatgata gaagaaaaag
     2821 caattgatat gtcaaagttg tggcaatacc atgctaactt agagtaggtg aaaaatgaaa
     2881 gcggtacatt ttggagcagg gaacatcggc agagggctta ttggacaaat tctacatcag
     2941 aatggattcg atctatgttt tattgatacg aatcctgaac tgatccatca attaaatcaa
     3001 gataagggtt atactatcga aattattgat gaaatgaaca ggagtgattt cattgatcat
     3061 gttgtggcac tgaatagttt atccgagcta gaagaggtga ttacagcaac aaaaaatgca
     3121 gatatcttaa caacatctgt tggcgcaata aatttagtaa aaattgcacc gatcattgcg
     3181 caagccttaa gtaagcgatt tgaacaaaag catagcatca atatcgtagc taatgaaaat
     3241 gtgataaatg caagtgatgt gttgaaaaaa gaaatttatt ctttgctgtc agaaaaagaa
     3301 caagagaatt ttgatgaata tgcatatttt gtgaatacgg caatcgatcg acaatcttta
     3361 ggaaaagttc ttggaggaaa aatgatctct gttgtcgaac cttattatga atggataatg
     3421 gatcgacagc agttagatcc aactacatct tttgatatga aaaatgtaaa tttggttgat
     3481 gatatggagc cttacattga gcgaaagctt tatatcgtta atgctgctca tgcggcagta
     3541 gcttatttag gcgcactgaa aggatataag acgattcaag aagcaatcca aaatatggag
     3601 attttcaatc tagtcaagca atttttaatg gagaatatag agtactttgt acaaaaatat
     3661 catttttcta gagaagaatt aacatctttt gtggaaaaaa cattaaaccg tcatgggaat
     3721 cagaaactct ccgatgaaat tactcgtgta ggaagttccc ctattcgaaa attaggccct
     3781 aatgatcgtt taattgctcc catagtaaaa ctagattcat atcatttacc ttacgatgca
     3841 ggaataaaag taattgctgc gggatatcat tatcggaatc ttgctgatcc agaagctgaa
     3901 caactacgtc gaatgattgt aaatgatggg ttgaaagcaa cgattaaaaa gatttctcgt
     3961 ttggaaggta agctgctgaa tgaagttgtc gctgcttatg agtcccagat ttagaggagg
     4021 agaatatgct aaaagaacaa ttgtcaggaa atacaatgtt tatggatgcg atcgattcat
     4081 ggcaagaggc aatccgagta agtgcgcgtc ctttgatagg aaagggaata atagaggaaa
     4141 gctatgtaca agcgatgata caaaatgtgt tggataacgg aaattatatt atattactgc
     4201 cactagtagc aatgcctcat gcgcgtccag aatttgggag caaaggggtc gggatgagct
     4261 ttttatgttt agataagcca gtcatgttcc caagtgaaga accagtaaaa ttcttcttta
     4321 ccttttcttc tgattcgcca aagggacatt tagacttaat ggccaatctt ggagaattgc
     4381 tgagtgatga aaaaatgtat caaaaattat tcgatgtgaa gacagaaaaa gcattgttag
     4441 atttgataaa gaagtaaagg agagaagaaa atggaggtag caaacaaaaa aaaacgagtc
     4501 tccatgcgag ctaaagttca agcgattggc ggtttcttaa caaatatggt attgccaaat
     4561 ataggcgcat ttattgcatg gggaattgtt actgcattat tcattccaac tgggtggttc
     4621 ccaaatgaat ctctgaatga actcgtaagt ccgacagtca gctatctatt gccgttacta
     4681 cttgcctata ctggtggtcg gatggttggt ggtacacgtg gagctgtttt aggtacaatc
     4741 ggaacaatag gtttgattat tggtgctgac ataccaatgt tcatgggagc aatgattatg
     4801 gggccacttg gtgggtggat gatgaaaaaa acagatgatt ttttagatgg gaaagttcct
     4861 gctggttttg aaatggtcgt caataatttt tctataggaa ttattggttt catactgatg
     4921 tgcctttctt atctggtagt tggaccaata atccaatcag ctaatcaatt tgtaacgagt
     4981 gcaattgaag cattagtttc aactggtttt ttaccgatac tgtccttaat caacgaaccg
     5041 gcaaaagtct tatttttaaa taatgttatt gaccaaggtg tttattatcc attaggtatg
     5101 caacaaacat tagaggcagg cagatcaatt tatttcatgg tagcatcaaa tcctggccct
     5161 ggcttaggac tattgttagc ttattcttta tttggtaaaa aggcagcaaa acagacagca
     5221 ccaggtgcaa tcatcatcca ttttttcggc ggaatacatg agatttattt tccatatgta
     5281 ttaatgaaac cactcactat attatctatg attgctggtg gaatgacagg aatcatcact
     5341 tttgaattat ttgatgcggg cctggtagca ggtccaagtc caggttctat tttttcttat
     5401 ttagctctaa cgcctcgggg aaattttttg ggtgtcatgg cgggagtggt cacagcaaca
     5461 ctggtttcct tttttgtaac atcagtaatt ttaaaagcag ataaatcaac agatgaagac
     5521 agaaattttg aactatcagt tcaaaaatcg aaaacgatga aatcagaagg aagaaaatta
     5581 ttaaaagaag aaaatgctag agaaatatgt accgagaaaa aagagatgaa taaaatttcc
     5641 tttgcttgtg atgcaggaat gggaagcagc gcaatgggcg caacaatatt caagaaaaaa
     5701 ttgcaaaaag caggtattac tgatgttgag ataaaaaatt atcgaattga agaggtacca
     5761 gaagatagtg atttggtgat cgtacatcaa gatttggagg aacggactag aaaagcacat
     5821 cctaacatga aaattgtgac attaaagaat tatttgcaag atccagtaat caatggatta
     5881 gcagaagaaa tcattggatt aaaaacagaa gactaattta tgggaatttt tgattaacct
     5941 ttattaaaat tattttgcct aatataaaaa ggttgtgaca tggcttgtgt cgcaaccttt
     6001 ttttgtcgca caaataatta atcggatatt ttatgttcat attttttgtg atcaacgaga
     6061 actgatgggg caaagataca ttttagatac ttttaactgt taatttacaa aacaaggtga
     6121 aacgaataga aaaatgaaaa aatcgatacg gaagaatatg tttgattaaa acatgcatga
     6181 agagaaggag gttgatgata catgaaaaaa atatattcaa aggaaatcga ttgttttaga
     6241 ggcattgctg ttttagcagt gatattttat catttgccaa atagtttgct gccaggcgga
     6301 ttgcaaggag taacgatctt ttttgtggtt tctggttttt taatggctaa aaatacgata
     6361 agagatgaaa aaaacagaca attcagtttt ttacattttt actataagcg tatcaaacgt
     6421 atttatccat ttttactgtt ttctttggtc gtattcattt tcatactagg gattagcaac
     6481 attcgattga taggaaacat tcgtagtgag ctacccagtt tgatttttgg ctataacaat
     6541 tggtggcagc tgaatcaagg agtatcttat tttgactctt actttaatgc ttctttattt
     6601 aaacattatt ggtcgctttc agttgaacta caattctatt tgatatggcc atttttgttt
     6661 attgtcatta agcgtatgag aagaaaacaa gtgttttact taatctatac attgattttt
     6721 gtttctattc tttttagttt gtttttacct tcaactaagg catattatca tacgatagct
     6781 aaactttttc catttttatt aggagtttgg gggtatttta atcgaataac tattggccgt
     6841 ttctttgaac aaaacacttt tagtaaaatc tggcttttat tactagctag tctttgtctg
     6901 attctgttcc cgatttttcc ttatacacta aatgaactac tgatcagtat ttgttttgct
     6961 ctattgttag ccagtgtcga tgatatgaac attgcaaaaa agaccaaaat cgctagtaca
     7021 aaactttctt ttgtaggaaa aatcagttat gaactttatt tggtgcattt cccagtattg
     7081 attgctctga caaatctatt caaacatcac cattggaaat atttggatat cgcaggtcta
     7141 ttcactgtgt ttttatgtac attactcatg attacttgct ttagaaaagg agtgcaaaag
     7201 atgagatcac ccaaaatttg gacagctatg gcactaatga taatcggagt ttctgtactg
     7261 gtattacgtg ctcctgccag tcgactgacc gctgaccaaa atcagttgca aagtttatta
     7321 aaagaaaact ctagtaaagc aaatgcagga gaaaatctgt tgtcacaaga gaagaaaata
     7381 ttatttatcg gtgattctgt tatgctgggg gcgtatcagg aactaaagga gacatttaaa
     7441 aaagaagcat tggtagatgc aaaagaatca agacaaatca ctgcgttacc agaaatctta
     7501 aaggagcata aagatttagt ttcttaccaa caaatagtga tcgggttagg aaccaatggt
     7561 gtgataacag atgaagctat agaacaaaca atgtctttgc tgaaagataa aaaagtatac
     7621 tgggtaaata taaaagcacc aactggctgg caagatacga taaatgccac attagctaca
     7681 ttgccaaatc agtattctaa tataacgatg atcgattggt atagtgagag caaaaatcat
     7741 cctgaatttt tttatgatga tgaaacccat ttgaatgaaa gtggacgtac agcttatgca
     7801 aagtatattg cttcagttat tcaataaggt agtgattacg tgtgttaacc ttactttttc
     7861 ctaataggtg aatcaattta aataaaaaat acttcatatg ataaacggtg tttaagagga
     7921 ggagcttttt aatattcgtg gtaaaaaaga gatgggcgag gtcgttaact gagagcttct
     7981 tggaaagaaa tcgctttgga tttatcctct ttgttataca gaacatgagc gagacgacaa
     8041 tactaggatg tatgttctga tcgaagtttt atggttacaa gcagcaatca gcttagataa
     8101 aattaaggat cgtaggaacc aagtaaagaa ggctgttagt aaagaaacaa acgatcaagt
     8161 atgttttact cttaagaaga tgcagtgaat aaataatagt aatataatgc tagaagggac
     8221 tgtgacaaaa gacgtgtcac agcctttttt gttattttaa ttatcgtcca gtcttttttt
     8281 ataaatgatt gcttaagcat taaatattaa aaaatataga gttcaaaatt actgcacaat
     8341 tagatgaaaa atttgaacaa caaaaagtgt atttttatac tataatagct tttgaattta
     8401 gttactctat tcaaaagtta tgctatggac aagaaaaaga ataaatagag gtgagaaaat
     8461 gaatagtttc aagaaaaaat ttgtgagtag atttttaaat atgaaaaatt tttttgaaaa
     8521 aaagggagat tatgatgcat ataattatgt attaaatcta gaaaagcaaa catattatac
     8581 actacgtaaa aacgatgata atgacttttg gactatgtta cctaaaatgc tttcgattga
     8641 ctctaagctt tcccttttag aggaatttat taatttagac attgaagaat tttgcggggg
     8701 aaaagagtta attaatatgg tagagagtga ttatcatacc tattataaag aaagttgtgg
     8761 atacaaagtg gatgagcaat gtccacaatc aattattttt tacattgagt aatatgaact
     8821 attaagcagg aaagcaacta gaagctactg atttagtatt tacaaatata aactatattg
     8881 ctaacaatta ttttctagaa ggaataaaag aactaagaat caagacgtta tcttgattct
     8941 tagttctttt aacatggaaa agatggataa tgcactgtta attcatgatg catcatcaga
     9001 aaagttggaa ggagaagata gaaatcatta aatgatttct acagatggta aatgaaaaaa
     9061 caaaatatta gcaaaaattt gcctaaatag atagtaacac tttctaaatt aaatacaagc
     9121 gcattcataa aatgtttata caaatatcta tgtgataaaa attggatatg ttcacaataa
     9181 ataggaatta aaataacgaa tttacgtttg tttttattta ccaatcaaat tatcttaatg
     9241 caggaataaa aaattaaatt acatattgtt aatcatttac atctccatta ataactcaaa
     9301 atgaaaagat gtttttattt ttattctata tttcagaaaa aaattaaact agattataat
     9361 aattcttcat gtaaaaataa aaacaattac agctatgatg tttaagataa aacaattatg
     9421 aaatcgatat ccgtttatat acttatcata agctagaagc aaataactag cctatgtatg
     9481 taagtgagat tatatttatt aaaaggcttc ttttgttcga tcagttatag tcgaacgaga
     9541 gaagtt
//
LOCUS       DAFCNS010000090         8731 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFCNS010000090 DAFCNS010000000
VERSION     DAFCNS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.092"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..74
                     /locus_tag="LNE23_002418"
     tRNA            2..74
                     /locus_tag="LNE23_002418"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="LNE23_002419"
     tRNA            78..150
                     /locus_tag="LNE23_002419"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="LNE23_002420"
     tRNA            155..225
                     /locus_tag="LNE23_002420"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="LNE23_002421"
     tRNA            238..311
                     /locus_tag="LNE23_002421"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="LNE23_002422"
     tRNA            323..394
                     /locus_tag="LNE23_002422"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="LNE23_002423"
     CDS             660..1853
                     /locus_tag="LNE23_002423"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBK6932242.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="LNE23_002424"
     CDS             1983..3455
                     /locus_tag="LNE23_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6932243.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="LNE23_002425"
     CDS             3538..4326
                     /locus_tag="LNE23_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBK6932244.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="LNE23_002426"
     CDS             complement(4393..5061)
                     /locus_tag="LNE23_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBK6932245.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="LNE23_002427"
     CDS             5176..6888
                     /locus_tag="LNE23_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6932246.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="LNE23_002428"
     CDS             6891..8663
                     /locus_tag="LNE23_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6932247.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2622 a         1487 c         1944 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc a
//
LOCUS       DAFCNS010000091         8586 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFCNS010000091 DAFCNS010000000
VERSION     DAFCNS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.093"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1511)
                     /locus_tag="LNE23_002429"
     CDS             complement(45..1511)
                     /locus_tag="LNE23_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBK6932248.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="LNE23_002430"
     CDS             1898..2494
                     /locus_tag="LNE23_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK6932249.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="LNE23_002431"
     CDS             complement(2552..3034)
                     /locus_tag="LNE23_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932250.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="LNE23_002432"
     CDS             complement(3140..3337)
                     /locus_tag="LNE23_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932251.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="LNE23_002433"
     CDS             3632..4390
                     /locus_tag="LNE23_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBK6932252.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="LNE23_002434"
     CDS             4450..4878
                     /locus_tag="LNE23_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932253.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="LNE23_002435"
     tRNA            4924..4996
                     /locus_tag="LNE23_002435"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="LNE23_002436"
     CDS             complement(5146..5517)
                     /locus_tag="LNE23_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6932254.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="LNE23_002437"
     CDS             5732..5917
                     /locus_tag="LNE23_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932255.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="LNE23_002438"
     CDS             complement(6000..6188)
                     /locus_tag="LNE23_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932256.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="LNE23_002439"
     CDS             complement(6212..6475)
                     /locus_tag="LNE23_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932257.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="LNE23_002440"
     CDS             6633..6890
                     /locus_tag="LNE23_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBK6932258.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="LNE23_002441"
     CDS             complement(6961..7152)
                     /locus_tag="LNE23_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932259.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="LNE23_002442"
     CDS             complement(7293..7613)
                     /locus_tag="LNE23_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932260.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="LNE23_002443"
     CDS             complement(7636..8070)
                     /locus_tag="LNE23_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6932261.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..>8586
                     /locus_tag="LNE23_002444"
     CDS             8405..>8586
                     /locus_tag="LNE23_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6932262.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2718 a         1694 c         1362 g         2812 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattc
//
LOCUS       DAFCNS010000092         8490 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFCNS010000092 DAFCNS010000000
VERSION     DAFCNS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.094"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..173)
                     /locus_tag="LNE23_002445"
     CDS             complement(<1..173)
                     /locus_tag="LNE23_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6932263.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="LNE23_002446"
     CDS             complement(593..1714)
                     /locus_tag="LNE23_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBK6932264.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="LNE23_002447"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="LNE23_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBK6932265.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="LNE23_002448"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="LNE23_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBK6932266.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="LNE23_002449"
     CDS             complement(4304..5215)
                     /locus_tag="LNE23_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6932267.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="LNE23_002450"
     CDS             complement(5232..6239)
                     /locus_tag="LNE23_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK6932268.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="LNE23_002451"
     CDS             complement(6236..8365)
                     /locus_tag="LNE23_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6932269.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8490)
                     /locus_tag="LNE23_002452"
     CDS             complement(8370..>8490)
                     /locus_tag="LNE23_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6932270.1"
                     /translation="GQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2677 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg
//
LOCUS       DAFCNS010000093         8388 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFCNS010000093 DAFCNS010000000
VERSION     DAFCNS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.095"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            31..1185
                     /locus_tag="LNE23_002453"
     CDS             31..1185
                     /locus_tag="LNE23_002453"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBK6932271.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1207..1959)
                     /locus_tag="LNE23_002454"
     CDS             complement(1207..1959)
                     /locus_tag="LNE23_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6932272.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2323..4989)
                     /locus_tag="LNE23_002455"
     CDS             complement(2323..4989)
                     /locus_tag="LNE23_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6932273.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5331..6782
                     /gene="cls"
                     /locus_tag="LNE23_002456"
     CDS             5331..6782
                     /gene="cls"
                     /locus_tag="LNE23_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK6932274.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(6987..7802)
                     /gene="thiD"
                     /locus_tag="LNE23_002457"
     CDS             complement(6987..7802)
                     /gene="thiD"
                     /locus_tag="LNE23_002457"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBK6932275.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            8096..8371
                     /locus_tag="LNE23_002458"
     CDS             8096..8371
                     /locus_tag="LNE23_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932276.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         2488 a         1727 c         1488 g         2685 t
ORIGIN      
        1 gttttttatc ataagtaaag aggtgttgaa atgaaaatag ggattgatcg tctttccttt
       61 tttattccta atttatattt agatatgaca gagttggcag aaagccgtgg ggatgatcct
      121 gcaaaatacc atatagggat tggtcaagac caaatggcag tcaatcgtgc aaatgaagac
      181 atcattacac taggagcaaa tgctgccagc aaaatcgtaa cagaaaaaga ccgtgagcta
      241 atcgacatgg tcatcgtcgg aacagaatcc gggattgatc attcaaaagc gagtgcggta
      301 attatccacc atttactgaa aatccaatct tttgctcgtt cttttgaagt aaaagaggct
      361 tgctacggtg gcaccgcagc tttgcacatg gcgaaagaat atgtcaaaaa tcatccagaa
      421 cgaaaagtac tagtcatagc aagtgatatt gctcgttacg gcttggcaag cggtggtgaa
      481 gtgacgcaag gtgtcggtgc tgttgcgatg atgatcactc aaaacccgcg tattttatcg
      541 attgaagacg acagcgtatt tctgacagaa gatatctatg atttctggcg tccagattat
      601 agcgaatttc ctgttgttga tggtccttta tctaattcta cgtatatcga atcattccaa
      661 aaagtttgga atcgacataa agaattgtcg ggtcgaggac tcgaagatta tcaggcgatt
      721 gctttccata ttccgtatac taagatggga aaaaaggcat tgcaaagcgt attagaccaa
      781 acagacgaag aaaatcagga acgtcttatg gctcgctatg aagaaagcat ccgttacagc
      841 cgacgaatcg gtaatcttta cactggttca ttatacctgg ggctaacttc tctactggaa
      901 aattcgaaat cactacagcc aggagatcgc atcggtctat tcagctacgg ttctggtgca
      961 gtaagtgaat tttttacagg ttatctggaa gaaaactatc aagaatatct cttcgcacaa
     1021 tctcatcaag agatgctaga ttctcgaaca cggatcactg ttgacgaata tgaaacgatt
     1081 ttcagtgaga cgcttcctga acacggagaa tgtgcagaat atacttcaga tgtacctttt
     1141 tcgattacaa agatcgaaaa tgatattcgt tactacaaaa tataataaaa tcataaaaag
     1201 aacccttcac gagtcgtgaa aggttctttt tttatctgcg gaccaaagga gcagtctata
     1261 ggtcagctta gtcgtactaa ggatttctcg tttttcactg aatagtttgc gaatgtacct
     1321 ggtcacaaca ggatcttttg cgtacgcgtc atagtatagc ttcatcaatt cttcagtaat
     1381 tgattcctgt gtaaaataag taaaatcttt tttcttcact ttgaatcctt catcaattaa
     1441 gtatgtttca aattgatcca ttacaactgg cgatgtatta ggatcttctt ctatcattcc
     1501 tagttgcttt tcgataggat aaaaaacatc atcgattgat tccactaccc tgattatcac
     1561 gcaccatttg cttgactttt tcatcaattt ttctaattga ataagatgta ccaatgctgg
     1621 atttgctgct gtaaaaacca tttcatatgt ctcatttgat tggataaatt cttctaacga
     1681 ctgtttgata aaggaaacat ttggcaactc atttttttct gcttcttttt ttgcattttc
     1741 tagcattcct gaagaaaaat cgatcaaatc atagcgatta atttcttttg ctaaggaaag
     1801 aaatctgcca gatcctccag caacgtctgc tgctgtttca gcaggcagga ctttctccgt
     1861 tttcagataa ttttttacat cttcagcaat agttacttgc gattcttgct ggatttcata
     1921 atattcttca gcaaattcat cccattcttt ttcattcatt tgatttcctc taattttttg
     1981 ttatatgttt tcacttttcg gaataaatta ttctcctctt ttatctattc tatctctttt
     2041 gatgaataat aaaatgattg gctttccgtc tatcataaat aaaaaaaggg actgtgacca
     2101 aagtcgtgtc aaagtccctc tttccgaata aacagtgtcc aatcagatga ccctcagtat
     2161 tagctcaaaa tgctgatttt acaacttctt ccttaaacgg tgttctcctg ccaactcttc
     2221 ggtcacaagt cacaatattc aacaaacatg agaagctgtt tgttgaaatt gcaccttgtg
     2281 actcagaatt aaacggcagg ctcacaacct cgttgattcg ttttatttat ttccttgtac
     2341 tcgtacaatg atcaatttag tgatttccat caaaacaatt gtcacaagtg cgattcccat
     2401 acatgtaaag acttctgtca atccaaatgc tgatgggata gagaagactt ctttcagtcc
     2461 tgggatcatt gttaagcagt aaagcgcgct acatacaact acagctagta agacacttct
     2521 gttagagaag aaccctgctt ggatagctgt ttgtgtgttt gaacgtgctg ggaatgtttg
     2581 caatgtccga caccaaatca atgttgaaaa tgacatggct acaccgaatt caggtgaatg
     2641 ctgcatgcca agccattgag caaagataac agcaatccca atcagcacac cacgatacgt
     2701 tacagaaatc aatgttttgt ccgtaaagat tcctgaattt ggatctcttg gcgggcggtt
     2761 catcgtagca ggttcagctt tttctgtacc taatgcaatt gctggcagtg aatcattgac
     2821 taaattgatg aacaacagtt gtaatgctgt aaatggattg gaccagtcag caactaaggc
     2881 aaagatgatc gcaatgatcg cacctaaatt tcctgcaaat agataagaaa tagctttctt
     2941 gatattatca aacacattac gtcctacttc tactgcgctg acaatagaag caaagttatc
     3001 atccgtcaat accatcgctg ctgcatcttt tgccacatct gtacccgttc ccatagcaat
     3061 tccgatgtct gcttgtttca atgctggtgc gtcatttaca ccatcacctg tcatagctga
     3121 gatatgacct ttattttgcc aagcacgaac gatacgaatt ttattctcag gtgaaacacg
     3181 tgcataaaca gtgacttttt ctaaaacttc gtttagttga tcttctgtca attcatctag
     3241 ttccgtcccg gtcaaagcca ggtttccttc ttcaaaaatc ccgatttgtt ttgcgatagc
     3301 aacagctgtt gttttgtggt caccagtaat catgattgtt ttgattcctg cacttcttgc
     3361 ttctgttacc gcctgagtca cttcagcacg tggaggatcg atcatagcaa gcaaaccaac
     3421 aaggatcaaa tcgtcttcat cttcaaatga taaatcaaaa ttatcaagtg gacggtaagc
     3481 aaaagctagt acacgtaatg cgcgtgttga aaaagcttca ttttgctctt ggaatttttt
     3541 catcaattca tccgtcatcg gaacgacttc accatttagc aatacttttt tactgcgttg
     3601 gaagacgata tctggtccgc ctttgacaaa caagtaattt tcgttatcga tcgtgtgtcc
     3661 agtagacatt aattttcgat cagaatcgaa aggaagttct gcttgtcttg ggtagttgtt
     3721 tcggatttcc tgataaggct ggtttaattt ttcactaaag tcgataaacg ctacttccgt
     3781 agggtcgccc aatttagtac cgtcttcact gatcgatgca tcattcgcta aaacactgat
     3841 ttcgatcaag cgacgttcgt cagctgtcca ttttgaaggg tcatttgtga aatccccagt
     3901 atcgccatta cctaaaaagt agtcaacaac tgtcattttg ttttgtgtca atgtaccagt
     3961 cttatctgta cagatgatac ttgtcgaacc aagtgtttca actgctggaa gcttccgaat
     4021 aatggcatga cgttttgcca ttttattcgt tcccaaagat aacacgattg ttacgataga
     4081 ctgtaatgct tctggaatag cagctacggc tacggctacg gcaaacatca ctgcgctgac
     4141 gatatctgca gtcatatctc ctgtaccttc tccaagataa atacgaaaca gttggatacc
     4201 taaaatcaat aaagacaatg ccaaaatagc cagacttagt tttttactga atttatccag
     4261 acctcgttgt aacggagtga cttgctcagt tgttgattcc aaaaggccag caacttgtcc
     4321 gatctccgta ttattcccag ttgaagtcac taagaattta ccgcgtccgt aagttacgat
     4381 cgttccactg aataccatat ttgtacgatc tccgattggt accgtacttg tgatatccgt
     4441 tacttctttt tctgcagaag tcgactctcc tgtcagcata ccttcatcta cttttaatga
     4501 tcccgcatct agtaaacgtc catcagcagg tacataatcg cctgcgtcta acaacacgat
     4561 atctcctacc acaagttcgt ttgccggaat cgttttttcc ttaccatcac gaatcactcg
     4621 agcatcgggc gctgaaaggt ttttcaacgc ttctaaagaa ccttctgctt tttttgtttg
     4681 gacaacactg attatagaat tcaacatcaa tactgcaaaa ataactaaga attcgatgta
     4741 gtcacccatg accatctgaa tcagtgcgac aatcagcaat acgataacca ttgcatcttt
     4801 gaaagtttct aagaacaatt tccaagttgg cttcttcggc ttttctttta gctggttcaa
     4861 gccatctctt tctaattttt ccttcgcttg cgtggtgctc aatccatcgg ttgagctctg
     4921 cgtttgctgc atcaacgagt cgatcgtttc tttatagacc gcttgttgtg cttgttcctt
     4981 gttttccaaa tttcatcccc cattttttga tcttggccct agtcctgtct gaaaaaaaaa
     5041 gagacctaag acaaatacgc atttgtctta ggtctcacca tttcatacaa taccagaaaa
     5101 ccatttggtt aacttactgt tgatattatc gcaacaaacg ttgccggtta ctcccctaag
     5161 gagatattca actgtactaa tatcctacct tattaaccta caaaaagcaa ctactttagc
     5221 ccgatttacc tacattttca tgaaaggagt gaaaagtata ttcaccttgg taggcttctt
     5281 tcttctatca tgctatacta atgttattgt atgtaaggag gaacattttt gtggtatcta
     5341 gtattataac cgccctttat ctattaaatg cacttattgc tttggtggct attttgatca
     5401 aaccccgaga tgtagcagcc atttgggcat ggctcttagt attgtttgcc cttcctgggg
     5461 tgggctttgt attatattta tttttcggac gtggattaac ggataagaaa aaattctatc
     5521 tccgacaaag tgacttgaaa gaattagaaa actttcagtc ttttagagga gataccattg
     5581 aacattacga tcctgacatg ggcgataaag acaaacagca atttgtcgac ttcttctctt
     5641 cattaaatcg tatgccgctg acaagaatga attctgtcac tcttctcaca gacggacaag
     5701 agaaattgga ttcactgctt caagatctaa aaaaagccaa acattcgatc catatcgaat
     5761 attacgcatt tgtgacagat aatatcggcc agcaagtctt acatgtttta gaagaaaaag
     5821 ccgcagaagg cgtggaagtt cgaatattat atgatgcatt tggctctcat ggtacaaaag
     5881 caaaagattt caatcgtcta atcaaaaatg gtggacatgt ccatacattt gttacctcac
     5941 aaaaggcatt acttcgtttc cgattgaatt accatgatca ccgaaaaatc gttgtgatcg
     6001 atggaaagat tagttatacc ggtggtttca atattgccaa tcaatatgta aatacaacaa
     6061 aaaaattcgg ctattggcgc gatacgcata tacggatttt cggtgccgct tcttctttgc
     6121 tccagcttcg cttcttaaca gactggaacg tctcggtacc tgaagaaaaa aaggtcggct
     6181 atcatttgaa ttatttcttt aaaaaagcag atcgagatga atctaagctt gctgatacat
     6241 ccatccagct tgtttcaagc ggaccgaata acgaaaggga acaaatcaag ctttcattta
     6301 tcaaattgat tacttctgct aaaaaacgtg tttggataca gacaccttac cttgttcctg
     6361 atgaaagtgt cattgctgct ttaaaaatcg caactgcctc tggtgtagat gtgaaaatca
     6421 tgattcccaa caaaccggat catcctttta tttatcgagc aacacaatat tatgctcggc
     6481 agctgatcaa ggaaaatgta caaatccttg tctatgagaa cggcttcctc catgcaaaaa
     6541 cattgataat ggatgatgaa atctgcatgg taggttcagc aaatcaagat attcgtagct
     6601 accgattgaa ttttgaaaca agtgctgtca tttacgatcc tgagttttta gaagaacttg
     6661 ctactcagtt caaagaagat gagacacatt gttcatccat gacaactgaa acagtcaagg
     6721 aaatgtctaa ctggctatta ttcaagcaac aaatttctcg attattttct ccaatcctat
     6781 aattttttta ttcaagtaga gaaaaatata ctaagattaa agggactgtg acaaaaatct
     6841 tgtcacagtc ccttcttccg aacaaacggt gttctcctgc caactcctcg gtcacaagtc
     6901 acaatattca acaaacatga gaagctgttt tttgaacttg ttccttgtga ctcggagctg
     6961 gacggcaggc tcacgacctc tttcttttac tctaattcac cagctgccca atgattgatg
     7021 ggtccatatt tgtgccctac atttatttct tcacgaattg cacggtttac atagtctttt
     7081 gccaatcgga tcgcttgttc aactgttttt cctttcgcta actcagctgc aatacatgca
     7141 gatagcgaat cgccagttcc gttgatccgg tcggtaggat aaaatggtgc atttaaccaa
     7201 aatgattttc ctgattcgag caatacgtag tcattgactg tttcttgctg cgcatcttta
     7261 gcatgatgtc ctttgatcat aacgtttttt gcacccatat cttgcagttc ttttgctgct
     7321 tgcacaaaat cttcttcagc tgctaatgtc cgtccagtca gcttttctgc ttcataaaaa
     7381 ttaggtgtca gtacttcggc caaagggaca agcttttctt tcaagctttg caaagcagat
     7441 tcttctagta aaagatttcc atgtttcgtt acgattacgg gatccacgac taatggtcca
     7501 aaatcatatt tttggtaatt ttttacaact gtctcaatca actttgaatc tgctagcata
     7561 cctgttttag ctgcacgtat ttgaaagtcc ttcgcaatca gttcaaattc tttgtcgata
     7621 aaatctgttg gcaatgtcac acttgctccg attccgtaag aattacctgc aacacatgcg
     7681 gtcatgacag aaacaccgta aacatttctt gtaaagaatg tatgcatatc tgcttgcatc
     7741 cctgcgcttc catcgctatc tgatcctgcg atcgtcattg cttgtacata ctgatcttcc
     7801 atcaaatcga ttcctccatt tttataaaaa gatttttcat aatgctgtta ctgtttctat
     7861 catatttttt tcaaagtatc cctcagtaaa tataccacta ccttttcttg gtaatcaaat
     7921 ttatttgaaa aaacatttgt aataaatcag aaaatagttg tcatttgttt gtattcgcta
     7981 ttctaataat aaataaccac tatttgattt atatatattt cagtatcctt cttgttttca
     8041 tataaccaaa ggcgctatga tttccatatg aaaaaaataa agtcaggtga aaaaaatgaa
     8101 acttgaggat aaaaaagtca ttttgaagaa aatcaaacga ctaccagacc aaatagatgg
     8161 ctatacgatc tgttctttca aaaaagacag gagtataaaa gcttatacgc aaaacacaga
     8221 aaaaggaata atctatttcc tacatgaaga aggttacgat acccaaacct ttatgtttac
     8281 tgaaaaaaaa gaatttcata aacagatgaa aaaactgatt gaaaaagaat ttccaagaag
     8341 tcataaactc tatgtgaatc agcaatttta acttctaact gttcttta
//
LOCUS       DAFCNS010000094         8352 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFCNS010000094 DAFCNS010000000
VERSION     DAFCNS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.096"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(507..848)
                     /locus_tag="LNE23_002459"
     CDS             complement(507..848)
                     /locus_tag="LNE23_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK6932277.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
     gene            complement(838..1119)
                     /locus_tag="LNE23_002460"
     CDS             complement(838..1119)
                     /locus_tag="LNE23_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBK6932278.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            complement(1226..1810)
                     /locus_tag="LNE23_002461"
     CDS             complement(1226..1810)
                     /locus_tag="LNE23_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932279.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            complement(1827..2066)
                     /locus_tag="LNE23_002462"
     CDS             complement(1827..2066)
                     /locus_tag="LNE23_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932280.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(2085..2333)
                     /locus_tag="LNE23_002463"
     CDS             complement(2085..2333)
                     /locus_tag="LNE23_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932281.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(2348..2914)
                     /locus_tag="LNE23_002464"
     CDS             complement(2348..2914)
                     /locus_tag="LNE23_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932282.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            complement(2915..3460)
                     /locus_tag="LNE23_002465"
     CDS             complement(2915..3460)
                     /locus_tag="LNE23_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932283.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3486..4253)
                     /locus_tag="LNE23_002466"
     CDS             complement(3486..4253)
                     /locus_tag="LNE23_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HBK6932284.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            complement(5142..5702)
                     /locus_tag="LNE23_002467"
     CDS             complement(5142..5702)
                     /locus_tag="LNE23_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HBK6932285.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            complement(5722..6579)
                     /locus_tag="LNE23_002468"
     CDS             complement(5722..6579)
                     /locus_tag="LNE23_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBK6932286.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            complement(6894..7241)
                     /locus_tag="LNE23_002469"
     CDS             complement(6894..7241)
                     /locus_tag="LNE23_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932287.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            complement(7234..8172)
                     /locus_tag="LNE23_002470"
                     /pseudo
     CDS             complement(7234..8172)
                     /locus_tag="LNE23_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
BASE COUNT         2383 a         1728 c         1105 g         3136 t
ORIGIN      
        1 gctacgcgac cgttcgttcg tcttttcctc cgtgggactc acccaccgca agcggttggt
       61 tgagttgtgg ctcctattca aaattcacta atatgttatt tttgttttct attcaagtgg
      121 gtaacgttcg tttgcaagca aactctctct atgtaacttg ccagttacat cagtggtaac
      181 gctacgtcgc aagcgacttg ctctaatgaa actaccgttt cattctgcct gttcgttcgt
      241 agcaagctac ttcactcact gtatccgtct gaattattgt tatttattac tattcaatat
      301 ccgtcagtca ttttatttat ttacatacat taaatataaa atgtatctta taattcgtat
      361 attactaatt atcactattt aacaatactt aatattttct taatttttat aatcacgtat
      421 atatttacgt gtattttaat atacataaat acgtttatat aaacgtatat cttaatatac
      481 atataaacaa aaaaagaaaa gccttgttag acttttcttt gtaatctctc gataaaccta
      541 tttagctttt tcgtatcaat agctgatagc ataccaacct ttttaaatga tatgttctct
      601 cggcttttgg gaatagcctt taaagaccct acgtcaatcc atgattgctt ctttagacct
      661 actttttccc attctgcaat aggaaaatac actctcttta tttggtctga tttattctca
      721 tattgacttg tgattccaaa aaattctact ctatcttctt tatcactaac caccaaaata
      781 ggtcttcttt taccaccatt tttatctgta aatgcaacat agaccgttac tatctcatta
      841 ggcttcattg aaccactctt ctaacttttc atctgtatct agaacatcaa ctggcatatc
      901 catagttagc tgttgcaatc tgattccttg cttttcctct tcgcttagct ttatctctac
      961 tggaaactca cctctagcga ccacttgctt ataaaggatt gtgattgctg ttgttggatt
     1021 taaccctagc tcttccaata tcatttctgc gttttctttt aaatccttgt ctattccgac
     1081 ttgaattctg cttttttctt ttggattttt taccttcatt tcgttcacct cttttgagta
     1141 taatatactc attattatat ccattaacta catttatgat tatattatac tcttaattag
     1201 ctactttgca aacaatccaa tcaaatcaag attctacttt actttttata cgttctttca
     1261 tcttctggta tgcaacctct actctttgga catctatatc caatccactt acaaccgtac
     1321 acataccaat ctcatcgcta tccgacactt tatcatattc ttcttcggtg atttcaatag
     1381 gcatataccc atcattaaag catatctcat catctgaata gttttcccat tcctcttcat
     1441 catcttctga ataagtgaca accctatctg ccccctgttc taccatatca tcgtaactat
     1501 cagcaacata ctgcgctttt aatcctttca gttgtaagtc atataaacca tacaacctct
     1561 tactatcttg ttcactgaat aaaattcttt gacgttctaa aattaattct ggttcatctt
     1621 ccacctcttc atctggttct gtaaaatacc attcgataaa ataatatcta atatcctcat
     1681 tcgataaact ttcaaagtcc atattctcct catcaaatcc atcaaaagcg ccactacttt
     1741 gcataccatt caaccaatta atggttctca atcttgcgta ttcacttaat accatcttat
     1801 tctcattcat ggttcttcac cccatattaa ttattttcaa attgacaaat caatttattt
     1861 tgagttaaca aaactaatag ctgttcacta tcaaaaagaa ccttacaaga ttctttttct
     1921 attctctctt ctggcatgta atagccaatc tgttttaaca tcttatctgg ataacctttt
     1981 tgtttggctt ttctcaaaag ctcttcatca ttcccaacat aataataaga acaccattgc
     2041 atacctttac ttttgaattt tgtcatgttg ctaccctctt tcgtttattg acccaaccat
     2101 gaaaaaatat attttgctaa atcttctttt tctatcccat ataaaacatt ctctaaatcc
     2161 aatttatact cttcatcaac aaggctttct gtattgtcat cataaaggac atagactgca
     2221 taatttcttt cgtaaaaggc attagcaaga cttctgctaa tacctaccat tcctttccat
     2281 gtataaccaa aatcaatcaa atcttcatga tctataaaat ccattccttc catgctgatt
     2341 cacactccta ttctgctgtt gtgtaatatg caacgcaatc atctgtccct actaaccaca
     2401 cacaccaatc ttcaatttca tcatcgcttc tcttactcaa ttcctctttg gtacaaaaaa
     2461 ctacttcacc attttctgtt tccatctcat aatcttgtag catgtattca aaccaacttc
     2521 tttcggttgg aaattctatc tcgtggcaac tctggcaaat aaaatcacca tattcattga
     2581 ttcggtagca accttcatct tctacaaatt catgattaca acaaacacat ctagtacctg
     2641 ttaggacttc cccattaatc gttgttctaa cggtatcttc tcttgctacg tgttcactgc
     2701 tgacaagcca atcaatgatt ttctttgcca attcttcatc agaatcggca aagcctttaa
     2761 acgtgtttct ctttccttga aagtagtatt tgcaacttgc ggtagtttct atggttccat
     2821 aacttttttg gaataattca atcatttcac catcaatcac tttttgactt ttcgcttcta
     2881 aattagggaa ctggtctttt cttttttcaa acacttattt cacgctctct tcttctaggt
     2941 ttattccata attttctagc aataatctgt atcccttaat aaaatctttc gtagcaaact
     3001 cttttccgct ttcacaaaac ttgtttagtc ctatctgttg accctcaaaa tcaaacaatc
     3061 gcaatctggt tttatcatct agactaaatc cattttcatc gaacaccatc tctaaatgag
     3121 catagccatc attgttatct gaaaacttca tcacatcttc aatcgtttct tgataaggtt
     3181 catagcgccc accaaaccct gtttcatcat aagcactcac tccagattca taggtgacaa
     3241 aattgataac tcctgtactt ctatcaaaga ttccttttgc taattcttct cttgatttat
     3301 ctcgtaagtc gtatgcttta ttgattaaat aaattccttg attcaatacg tccatcagca
     3361 tatatcgacc atacccccac tgataatgaa tcgacacgct tgctttcgtt ttgctgttac
     3421 catatttgac tgtaattaac gcttgtgttc tttcgcccat cttgttttcc tcttttctct
     3481 aatttttata aagaaccatt ttcttgaaat gagtaatagc ttgtgttttt acttgttacg
     3541 atgatatggt ctaacaattc aataccaatt gtttttcctg cgtttttcaa attcgtagta
     3601 aaacttttgt cattttcact tggaaattca tatccgctcg gatgattatg tgatactgcg
     3661 atttttgctg aattgtttag caaagctagt tgaaaaatct ctcttggtat cgctactgat
     3721 tgattaattc cacctttgaa aacttcgcta taagcaatga cctcattctt tgtattcaga
     3781 cacatcacta gcaaagattc actagcattg tttccaatta agttagtagc aaagtttgct
     3841 agaccaaccg agcttgtgac tctataaaaa cttctatctt cactgatata tttttgttcg
     3901 attttcaaga cattcttaat caatccttca ccttcattct cggtcttgtt ttcaacttca
     3961 atagattcaa gactgctatt tcccatatca gaacccaaca aagcatttct gaaatccaat
     4021 accttttcaa agttatcagt cataaccatt tcataggtgc tattccattc attcaaatat
     4081 ttctctttga tgagaatgaa cgctctcggt ttacttctgc caattctgat attttgtgta
     4141 tcgtgaaaaa tatagttatc aagtaaagta ccatcgtact tttcatagtt cccactgata
     4201 tttgttaact tgtttaccat ttcttgatta acttctaaaa ttctatctct catgttgaac
     4261 gcttctttct gtttttgatt tttggttttt caaaattctt ttttgtttgg attgttttag
     4321 agggaggtgg ttttttcttc atcgctattt ggttttgcat tttatgtttc ttcctttcgt
     4381 tcatttattt atctttttcg cctgtcttgc cttgtcggga gtgattagaa tgcgttctta
     4441 tctgccggat taatacaagt gtttaagtaa attattttcg taacgcttgc gtttagaaaa
     4501 aaatttacgc cacttgtatt aatccaaaga tgagataagc tctaaacact tctgacaagg
     4561 tatgacaggc gaaaaagata atgggcgtaa ggaattataa atggcaaaac caaataaaga
     4621 gcgatgaatt tcaatgcttt caggcgtagc ttgaaagcct aaaagtcttg aagaatgaaa
     4681 gactttttac cttcaaagca gacctagtaa tcttaaatag gcgatggcta tttaagatta
     4741 cggtgttgac ccactaaata aacagaaatg gaaccgatca agcaaaatcg gactaggcga
     4801 tggctaggac ggtttgcggt ggctttagga aacgttcatg gtgagatgag catatctagc
     4861 aatgtcgggc taagctatgc ttagaacggc ttgcggtggc tttagaaaac ttttatggcg
     4921 agatgcacta atctagcgat gtcggactag gcaatggcta ggacggcttg cggtggcttt
     4981 aggaaacgtt catggtgaga tgagcatatc tagcaatgtc gggctaagct atgcttagaa
     5041 cggcttgcgg tggctttagg agatgtttct atcatcatat ttcttcttaa atatagatga
     5101 atagaaaaat tacaagagaa ttttaagcat aaaaaaagag ttcaatattt agaactcttt
     5161 tttctaagat gctctggtaa catcttatcc aattcctctt ctgtaaaaaa ttgctcttct
     5221 tcagatttta atgctttctg agctgattca tagcttttac tatttgtttt aggggcattt
     5281 ttatatgctt catgagtttc gttcattaat tgatctcttt ttttcacaat atcttcaaca
     5341 cttaaacgat ttaaatctgt tagtaagctt acgtattctt ctctgatcaa ccataattta
     5401 tcggcaacaa tttgatgatt tttagcttct tcagcaagat caaaattctt aaagtataag
     5461 tttaatccaa gagaaattgt tgaacataat ccagcaacaa aggttaactc actattatca
     5521 gtaataatgg tactaagtac ccctccagta gtaagggctg acaaaccaat ttgaagacac
     5581 cttaaaattt tattgtcaga aataatatta tcggcttgct ttaaatgagt ggtgtaagta
     5641 tacgttactc gtccatatgc ttcacgtaat tgaacttcca agcgcttttt ttcgtctccc
     5701 atgttgttcc tccataaaac tttataaagc tatttttttt aatacttttt ctaaacatat
     5761 gatactacta tcagaattaa caaattgatt gcttccagga gagtataaat ttaaaccgcc
     5821 cataacttta tgtgagttat agtattctaa taaagctgtc tcataagata cagaactaat
     5881 gttttgaccc ccactgttat tttcaacgaa tttccaattt cccatagcct catatacgaa
     5941 actgtcaatt actattcctg aaagatgata actcgtaaat tctgtatctc gtacatgtcg
     6001 aaaatgttta cacgtagcat ataataaatt attacttttt gtattcttta atttcatggc
     6061 ttcttgctct tttttgggat ttgtagcttt ccagttccct cccatgtttg aatctggata
     6121 aataaaccct tttgactcac cccaataatc tttttcatta aaagctggaa ctatttcaaa
     6181 tttgattcca tcatggaaat ttattttaac aacttgtcca tcagctctaa tatcactatt
     6241 tggtagtatt tcctgaaatg cgttttttat aacttgtaat aacttagacg ggccatttga
     6301 taaataattt ttgaatctgt caaattctgc catcggtaat tcaactatta tatctatatc
     6361 actagtacta attgccgttc ctctcccata agaacctacg tataaactgt gagcagtatc
     6421 actttcagaa ttccaaaact ctctattgat ggctctagta atcctttgat atcttttagc
     6481 aattaattgg cgtttcgtta aaggtatagt ataagtatta gctctcggaa ccagtaaccc
     6541 cattataatc tcatcctttt taattaattt ttcttccata aattatgata tctaaatata
     6601 atacttcaag caatttattt tatatattac ttttataaaa aatttgatta attaaaggga
     6661 aatatttcac aataattgaa agtatttgga gaaaatttaa aaatatctaa gagactattt
     6721 tggtggatat ggaaatatat agtatctgtt cttatggtat cctgctccgg attcaatata
     6781 gaaacaaaag caatcaatcc ccacttcatt aattaattgt tgaccatatc cccataattc
     6841 ctctattagc tgttcatacg taatataacc gaattcgtat tttttaataa tgttcatatc
     6901 tgactccatt ttaataattc cgagaaagaa acattcctaa tttcatcata gtgaactttt
     6961 tcttcttcca caaatacacc aagctcgtca tatccaataa tttttcctac tacatcatct
     7021 ggatagtaac cttcagcgtt tcgttcctct ttttgaatgc agacgggtct attttttatg
     7081 acagcagttc tcagtatttt cgcaatctct tcagtcgtca tttgttcttt tgccaaatta
     7141 tgattagcac gttgtacatt gtctgcattg attctttcag tatgatcaga caaataaaat
     7201 cccgaccatt ttaaacgtcc tctatcgtta tattcaatca ttatgcaaac ctcctaatcc
     7261 tcctgcgtgt cctcctatta aagtgcttct cgtaatactt cttgcacctt ctaacataga
     7321 tgaagcatgt ataatagctg taaatccata tttttgtcta atcttatcaa taatcctatc
     7381 caaattttct tcagcaatct tttttcttgg atcactaaat aaatctaatt gtagggaatc
     7441 tgtgtatact aatctgctat aagttactcc tatatgtcgt acttcctgcc cctcataaaa
     7501 cttattgaat aaaaaaagac aataattttg taattctttc gtattatctg taattggtat
     7561 tttcatctgg tgcgagaaac ctgatttttc ttccaaaatt gacgtaccaa ttgctaagcg
     7621 tacacaacca gttttgcaat tgtgttgtct aattcgaatg gctacttgct ctgccatctc
     7681 cttgactact agttctactt cttctctcct cgtataatct tttggtagaa cttgtgaatt
     7741 cccataagac ttttctttgg ttggttctgg tggaatagca atatccgtac gatcaattcc
     7801 attggtatgg aaatataact ggagtccgat tacacctaac tgacttttaa ttgtgtaggg
     7861 attccaattt gctaaatctt ctatactcat aattcccata tgattcaatc tttttttcat
     7921 gcgtgatcca atgccccaaa agtcagtcat ctcgtttatt ttccatattt tttcaggtac
     7981 agtctcataa gtccactcag ctataaattc tttactactc ttcgcttcat tgtccagtgc
     8041 aagtttagct aataatgggt tatctcctat tccgactgtt gctattagac ctgtttcttt
     8101 ctgaatctca tctttgatta ttttagctaa ttctcttctt ctctcgcttc ttgttcctga
     8161 actatatagg ttgaattaaa tactttacat ccgcacgcac agtcggtcaa tcacctcaag
     8221 gggacgctcg tttacatcgg ctaagaaacc gcggaccgac tgtttgattt tctggacgtg
     8281 gtcaaaaaaa acattgttga tgaccgattc cttgagccac ttccaaacgc cttccattag
     8341 gttcaactga gg
//
LOCUS       DAFCNS010000095         8251 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFCNS010000095 DAFCNS010000000
VERSION     DAFCNS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.097"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..197
                     /locus_tag="LNE23_002471"
     CDS             <1..197
                     /locus_tag="LNE23_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932288.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="LNE23_002472"
     CDS             314..811
                     /locus_tag="LNE23_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK6932289.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="LNE23_002473"
     CDS             898..1290
                     /locus_tag="LNE23_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6932290.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="LNE23_002474"
     CDS             1274..3721
                     /locus_tag="LNE23_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6932291.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="LNE23_002475"
     CDS             3724..5907
                     /locus_tag="LNE23_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK6932292.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="LNE23_002476"
     CDS             5904..6905
                     /locus_tag="LNE23_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK6932293.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="LNE23_002477"
     CDS             6920..7834
                     /locus_tag="LNE23_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6932294.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8079..8195
                     /locus_tag="LNE23_002478"
     CDS             8079..8195
                     /locus_tag="LNE23_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK6932295.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2569 a         1568 c         1754 g         2360 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg c
//
LOCUS       DAFCNS010000096         8250 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFCNS010000096 DAFCNS010000000
VERSION     DAFCNS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.098"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            234..1559
                     /locus_tag="LNE23_002479"
     CDS             234..1559
                     /locus_tag="LNE23_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK6932296.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="LNE23_002480"
     CDS             1552..1902
                     /locus_tag="LNE23_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932297.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1884..2069
                     /locus_tag="LNE23_002481"
     CDS             1884..2069
                     /locus_tag="LNE23_002481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932298.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(2973..3248)
                     /locus_tag="LNE23_002482"
     CDS             complement(2973..3248)
                     /locus_tag="LNE23_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBK6932299.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3220..4173)
                     /locus_tag="LNE23_002483"
     CDS             complement(3220..4173)
                     /locus_tag="LNE23_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6932300.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4785..6278
                     /locus_tag="LNE23_002484"
     CDS             4785..6278
                     /locus_tag="LNE23_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBK6932301.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6413..6709
                     /locus_tag="LNE23_002485"
     CDS             6413..6709
                     /locus_tag="LNE23_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBK6932302.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6810..7661
                     /locus_tag="LNE23_002486"
     CDS             6810..7661
                     /locus_tag="LNE23_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932303.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7682..7965
                     /locus_tag="LNE23_002487"
                     /pseudo
     CDS             7682..7965
                     /locus_tag="LNE23_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2864 a         1283 c         1553 g         2550 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
     3001 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
     3061 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
     3121 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
     3181 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
     3241 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
     3301 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
     3361 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
     3421 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
     3481 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
     3541 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
     3601 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
     3661 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
     3721 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
     3781 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     3841 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     3901 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     3961 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     4021 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     4081 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     4141 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     4201 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     4261 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     4321 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
     4381 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     4441 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     4501 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     4561 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     4621 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
     4681 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     4741 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     4801 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     4861 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     4921 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     4981 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     5041 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     5101 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     5161 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     5221 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     5281 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     5341 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     5401 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     5461 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     5521 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     5581 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     5641 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     5701 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     5761 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     5821 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     5881 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     5941 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     6001 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     6061 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     6121 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     6181 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     6241 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     6301 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     6361 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     6421 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     6481 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     6541 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     6601 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     6661 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     6721 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     6781 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     6841 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     6901 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     6961 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     7021 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     7081 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     7141 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     7201 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     7261 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     7321 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     7381 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     7441 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     7501 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     7561 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     7621 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     7681 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     7741 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     7801 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     7861 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     7921 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     7981 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     8041 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     8101 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     8161 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     8221 cgtaagagaa attgatcgaa gatgtgacga
//
LOCUS       DAFCNS010000097         8068 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFCNS010000097 DAFCNS010000000
VERSION     DAFCNS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.099"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..884
                     /locus_tag="LNE23_002488"
     CDS             <1..884
                     /locus_tag="LNE23_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBK6932304.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="LNE23_002489"
     CDS             932..3061
                     /locus_tag="LNE23_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBK6932305.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="LNE23_002490"
     CDS             3036..3725
                     /locus_tag="LNE23_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932306.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="LNE23_002491"
     CDS             complement(4133..4819)
                     /locus_tag="LNE23_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6932307.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="LNE23_002492"
     CDS             complement(4955..5149)
                     /locus_tag="LNE23_002492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932308.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="LNE23_002493"
     CDS             complement(5199..5639)
                     /locus_tag="LNE23_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBK6932309.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="LNE23_002494"
     CDS             complement(5643..6488)
                     /locus_tag="LNE23_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBK6932310.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="LNE23_002495"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="LNE23_002495"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBK6932311.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1714 g         2358 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccgggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaa
//
LOCUS       DAFCNS010000098         7847 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFCNS010000098 DAFCNS010000000
VERSION     DAFCNS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.101"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(639..1985)
                     /gene="gor"
                     /locus_tag="LNE23_002496"
     CDS             complement(639..1985)
                     /gene="gor"
                     /locus_tag="LNE23_002496"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBK6932312.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2119..3372)
                     /locus_tag="LNE23_002497"
     CDS             complement(2119..3372)
                     /locus_tag="LNE23_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK6932313.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3378..4301)
                     /locus_tag="LNE23_002498"
     CDS             complement(3378..4301)
                     /locus_tag="LNE23_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK6932314.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4522..7014)
                     /locus_tag="LNE23_002499"
     CDS             complement(4522..7014)
                     /locus_tag="LNE23_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBK6932315.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7021..7488)
                     /locus_tag="LNE23_002500"
     CDS             complement(7021..7488)
                     /locus_tag="LNE23_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBK6932316.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7624..7697)
                     /locus_tag="LNE23_002501"
     tRNA            complement(7624..7697)
                     /locus_tag="LNE23_002501"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7661..7663),aa:Arg,seq:acg)
     gene            complement(7755..7828)
                     /locus_tag="LNE23_002502"
     tRNA            complement(7755..7828)
                     /locus_tag="LNE23_002502"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7792..7794),aa:Pro,seq:tgg)
BASE COUNT         2185 a         1705 c         1264 g         2693 t
ORIGIN      
        1 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga gaaactggct
       61 tttgaacacc gtttatcaga ggttgtaaaa tcagtgtttt gtcctgagta ttagagaaac
      121 aaatggaaaa atagttcctc atattttgcc gttttttgag ctaataccga aggacagctg
      181 attgaacact atttgttcgg aaaaagtgac taactatgac acaatttttt tcacagtccc
      241 ttttttcact tgatttgatt cgtaccagcg aacttgacga gaactttctt ggtttggtaa
      301 ctatatggta ttcttttaac atcctcgttt gcttcttcta atttatttat acaaagtcac
      361 acttttttaa acatctgttc ttcgatacat ttctatttct tttaaacatc gataccaata
      421 ttttgaactt aagagtgcat accaaatgga ttcatttact cttcgtcagt tacgaagata
      481 atcaaaatct ccaacttccc tacttatttc gttttgagaa ttatcgaagc aatcttacat
      541 aaataactaa aaaacaaaaa accagaaaat cagtatactg attttctggt tttacatggt
      601 ttgttcaata aaaagctgtt ggaactgtta atattaaatt atcgcatcgt tacgaattct
      661 tccgctcctg ttggatggat ggctactgta ttgtcaaagt cttctttagt tgcacccatc
      721 ttgatagcca cggcaaaacc ttgcagcatc tcatcgacac caacaccaat agcatgtaag
      781 ccgacgattc tttcttcttt tccgacacag atcaatttca tttcgcattt ttggcgataa
      841 tcatttaatg caaagtacat tggggtaaag cgtgaacgat aaactttcaa ttcattttcg
      901 ccatattttt ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg tggatgcgta
      961 aagacgactg tcggaacaag tttatagtca agataagaat ctttctttcc attaaataaa
     1021 cgttctgata aacgtctacc agctgcgatt gcaacaggag tcagatctaa tttaccgatc
     1081 acatcaccaa cagcaaaaat atgttcatcc gttgtatttt ggtatttatc tactttgata
     1141 aacccttttt tgtccaattc cacatttgta ttttccagac ctaatttttc ggtattcggt
     1201 acacgtccac cggcaaaaag tacacaatca ccagttaagg tttcaccatt ttcaaatagc
     1261 accgtatatt cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc gtaagttttt
     1321 attccagctt cttggtacat ttggatcaaa ttgtccgaaa gcattttatc aaacgttcta
     1381 agtggtcgtt cttttcgata tgcccacatg acttcactac ctaaaccacg aaatacacct
     1441 gaaagttcag ccgcaatgta tccagcacct aaaacgattg ctctcttagg taactctgtt
     1501 aattcaaaaa atccatttga atcaattgcg tattctccac caggaatggt cattttactt
     1561 gcacggcctc cagtagcgat caagatttta ggagcacgat aggtttcacc gtttacttcg
     1621 atcgtttgac tatcgataaa agtagcgtaa ccttttatag cctcgacatg attgctatct
     1681 aatcctcttt gataagcacc gtgtagaaaa tcaatatatt tttctcgtct ttctactaga
     1741 ccggcgaaat caacattttt taaatctgct tggataccgt aacttgaggc atctcgttca
     1801 attgtttcca aaatcgtact tgcttgccac ataacttttt taggtacaca accgacgttg
     1861 acacatgttc cgcccaattc attcgcttca atcagcaaaa cttttgcgcc atgcatccct
     1921 gctctgtttg ctgaagcaat accgccagag ccgccaccaa ttacgatgta atcatatatt
     1981 tgcattttgt tcctccttat tgaagagatc aatcaaaacc tttatttaac gatccttttt
     2041 ctacagaaaa caagtgtaac acttttcttg aattttgtat gcgaagatgc ttaaaacagg
     2101 gatatatcag ctttttcctt atttacgttt tcggatcata tctccaggtt tcaccggctg
     2161 tgcgactggc atagttaaaa tcatcattgg atttggtgct cggtcaatgg attcgccttc
     2221 ttcattatgc ataacttcaa cggtttgact gaaatgactg aatcctggac cataaaattc
     2281 gatttcatct cctacagaaa acaagtttct ctgacgtatc gtagcaatct gtgttgcctc
     2341 atcataagat aatacttcac cgacaaattt atatactggg attttacgac gttcaccgaa
     2401 taattgttcg ttttcagttg gtgtattgta gtaaaaacct gtagctaatt ctcgttgtgc
     2461 tactttccat aactcgtcga tccattcttg cttgcagaca tagttttcag gatcttccat
     2521 ataactgtct acagccgctt tatagacatt tgacaccgta gatacataat ggatcgattt
     2581 cattcttcct tcgatcttga agctgtctac gccgttttca atcaattctg gaatatgttc
     2641 gatcattgac atatccactg cactcatcga gaattcttct tccacttcgc ctgtatcagt
     2701 taaactgttt cgttcttgac caaaaggcat atcgaacaat ccatacttcc agcgacaaga
     2761 ttgagaacat ccgccacggt ttgcatcccg catcgacata tggttggaca atgtgcatcg
     2821 accagaataa gagatacaca tggcaccatg gataaatgct tcgatttcgg tatccgtatt
     2881 tttacgaatc tctgccactt cctccattga aacttcacga gctaaaacga cacgttccaa
     2941 cccttcttct ttccagaatt ccaatgtctc aaaattagtt gctgaagctt gagtggataa
     3001 atggatcggc agacctgggg cttctgtcgc acaaatttcg atcaaagctg gatcagaaac
     3061 aatcactgca gaaataccga catcacgcaa actgcggaaa aattcccctg ctccatcttc
     3121 atttccttca tgggtgacca tattagcagc aacgtatacc ttcgcatcat gttcttttgc
     3181 aaaagcaact ccttcagcca tttcttcata agtaaaatta cctgcacggc ttcttaaacc
     3241 atatgcattc ccgccaatat atactgcatc tgctccataa tggatcgcgg tttttaactt
     3301 ttccagtgtt cctgctggcg ctaggatttc tggtcgtttc aattttcttt ttactgtcat
     3361 ttctttcatc atactcctta tttgatagca gaaggatcaa tataaaagaa tcctgtatct
     3421 aattcacggt tttcaggatg caatttactg atttcgctag tcattttttc cgctttttct
     3481 tgatcccagc taccttctaa aatttgtttt tttgcttcgg cgaatagtcg agtgatgttt
     3541 acgaattttt cgcctggagt aaataatccg tccaatttcc atgtacagta atgatgctga
     3601 taaagttcat tcaattcatt cgagagattc agatcattgc tggcaaaaat atgtgtgcca
     3661 tgactatctt cataaataga ataatgtgtc tcttcttttt taggttccga gataaaaaga
     3721 cctctttcac gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt ctgtagcagt
     3781 ggacgtttgg attgatggat gcatgtagca ccatacacaa gggtttctac aggaatatcg
     3841 agtttttctt ccatcgcctt catctcttca aaaggaactt cacgagcaag tactgctcca
     3901 actgcgcctt ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa tgtttcagca
     3961 tcataaatat acggtatacg caattcagga ttttttttca tcgtataaat aatacctgta
     4021 tcaccaactg tgatcacatc tacgtttatt tcttttaaaa aagccaaata ttcaggtaca
     4081 tttttcattt tttcaggatg aatgatccca tttacagcaa tattcgcttt tttaccatac
     4141 ttgtgtgcta attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc aggcagacgc
     4201 aatccaaatt gctcttcacc gaaatatagt gtatctgcac ctgcagcaag caactgttct
     4261 gcttgttcaa tagtttctac tgtggttatg agttcgatca taattttctc ctcctacaac
     4321 ttcataatag tagcatagct ctaaaaactt tccaatactt cttctattta aagtcggatt
     4381 tttgaaaatt atttcacaaa aaatcaaaat aaagttcgat atattctgtc caaatataaa
     4441 aagagattgt gacaaaaatc ttgtcacaac ctcttaatca agtcttacta ttataaaaat
     4501 tcagctacac aggccaagta actataggga acctactgtt tcctttttag gtccgcgaac
     4561 attcaatgtg atcttacctt ttgaagcacc tactgttacg tggtcaccta aacgaatttc
     4621 tccgccaagc aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg cgcgacggat
     4681 tggacgtgca ccatattcag gatcaaatcc agctttcccg atcacatcga ttgcagatgg
     4741 tgtgattttc acttgaatat cttgttcttt caatcgtttg ataatcgctt tgctcatgat
     4801 ttggacgatt gtatggattt catctttact caatgaatgg aagacgacag tctcatcgat
     4861 tcgattcaag aattcaggtc ggaaagcttt ctttaactct tctaagatac gtttttgcat
     4921 ggctttgtga tctttagtaa catcttggac attgaatccg acattttttt cttctcgaat
     4981 ttgtgtcgct ccgatatttg aagtcataat catgattgta tttctaaagt ccacttttct
     5041 acctttagaa tctgttaaat gaccatcatc caatacttgc agaagtaaat tgaaaacgtc
     5101 tggatgtgct ttctccactt catctaagag aatcactgaa tatggttttt gacgaatttt
     5161 ttctgtcaac tgtccacctt catcataccc tacgtatcct ggaggagacc cgattaatcg
     5221 gcttgtactg tatttttcca taaattctga catatcaaca cggatcaggg catcttcact
     5281 tccaaacatg acttctgaaa gtgctttggc caattctgtt tttccgacac cagttggtcc
     5341 aaggaacatg aatgaaccaa ttggacgatc aggatctttc aatccgcttc gtgcacggcg
     5401 gatcgcacgt gaaactgctt tgacagcctc atcttgacca acgacacgtt catgcagaag
     5461 tccttccagt tctaacaagc gttcgctttc ttttttctcc aactgctgta gagggacacc
     5521 agtccactcc gatacaactg tagcaacatc ttcttctgtt acacgatcag aataaccttg
     5581 ctcctgtttt gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg ctttttcttg
     5641 tctgcgtaat tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt ccttttcttg
     5701 tactagttca tcgatttcta gtttgatcac agttgatttt gttaagtgat cggtttgatc
     5761 caaacggact ttcgctgctg attcatcgat caaatcaatt gctttatcgg gtagttgacg
     5821 agaagtgata taacgaacag aaagattgac tgctgctcgt acagcttcat ctgtgatctc
     5881 cactccatgg tgctcttcat atcttgtacg taatccttga agaatcactt ctgcttcttc
     5941 aggagttggc tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag aatctttttc
     6001 gatatatttt tgatattcat ctagggtcgt tgctccgatc gtttgtaatt ctcctcgagc
     6061 taacgccggt ttcaagatat tcgaagcatc aattgctcct tctgctccac ctgcaccaat
     6121 taaggtatgc aattcatcaa tgaataagat tacttgacca tcattataga tttcgtcgat
     6181 gacctttttc attcgatctt caaattcacc gcgatatttc gtaccagcaa ccaaagatcc
     6241 catatcaagc atcatcaggc gcttttcttg catgtcttct ggcacttcac ctaaaatgat
     6301 tttttgagct aacccttcag caatggctgt tttgcctaca ccaggttcac ccaccagtac
     6361 agggttattc tttgtccgtc ggctaagaat ctggataagc cgtttcactt ctctcgaacg
     6421 accaacaact gggtccagct tgttttcttt tgctaatttc gtcaaatcac gtgctaatga
     6481 atctagcgta ggtgttcctt gcggtgtttg ccgtttatct tgtacattta catttctacg
     6541 acgattcatt ccttttccag cagaatcacc agctagcccc attttttgtt tcagaacttg
     6601 acgggctttt gaaaggctga tacctaagtt gatcatgatt cgagtagcca tgatactttc
     6661 atcttttaat aagctcatca aaatatgttc tgttccaact ttttgagaac cagttttctt
     6721 cacttcgctg ctagctaaag caaaaacttg tttcattctt ggtgaatatg gaagaaagac
     6781 tcctttaggg taagaacgga taccaccata accaatcaga tgttcaattt cttcataaat
     6841 ttccgtttca ttcaaatcca tttcacgtaa tacttttcca gcaattccat tttgctcgac
     6901 cactaaggct agtaaaacat gttctgaact aactgattga tgtttcaacc gttttgcttc
     6961 ttcttgtgca atttctaata ctgcttgggc tctttcagta aaaagttctt ccatctaatc
     7021 tcacttccca tcttcataac ttaaacgttc cagaaatgct cggagaatcc gagcacgtgt
     7081 atgatcttca tattcattga aattcaatgt gttttttgca attgcactca acatcaagtt
     7141 tccttctttt tttgtaatta tttggtcttc ataaagtttt tgaataatag caaaagcatt
     7201 tttctcactg attgtatcat caaataactg atttatctgc tcaagtaatt gttttttatc
     7261 tgaaatcctt acttttgcaa ttcgtatata accaccacca ccacgtttgc tttctactag
     7321 ataaccacgt tggatggtaa aacgggtatt aatcacatag ttgatttgtg aaggaacaca
     7381 gttaaagagg tctgccattt caatccttcg aatctcgatc atttcactat tttccagaat
     7441 tttctttata tatgattcaa ttaaatccga ggtattctga tgtgccatct tattcattcc
     7501 ctttgactaa tattgacctt aattataacg aatttgataa tagaaagaaa gaagaacgct
     7561 ttttgttaaa taaatccttt agaatcaaca aaaaaaaata cggaatatac tattccgtat
     7621 cgacgcgccc aagaggagtc gaacccctaa ccttttgatc cgtagtcaaa cactctatcc
     7681 agttgagcta tgggcgcata ttcaatttaa aaaaattata aatcatttga aaacaaaaag
     7741 caagataaaa aaaatcggga agacaggatt cgaacctgcg accccttggt cccaaaccaa
     7801 gtgctctacc aagctgagct acttcccgtt taaaaaataa aaaagac
//
LOCUS       DAFCNS010000099         7757 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFCNS010000099 DAFCNS010000000
VERSION     DAFCNS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.102"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            251..478
                     /locus_tag="LNE23_002503"
                     /pseudo
     CDS             251..478
                     /locus_tag="LNE23_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            604..1575
                     /locus_tag="LNE23_002504"
     CDS             604..1575
                     /locus_tag="LNE23_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6932317.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            1716..3029
                     /locus_tag="LNE23_002505"
     CDS             1716..3029
                     /locus_tag="LNE23_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK6932318.1"
                     /translation="MKRRMLAIAAILALTAGGLAGCGSKEKSSGSSDSGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFVWSNGGDVVNADGTKAEDYFNDKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIISLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3072..3938
                     /locus_tag="LNE23_002506"
     CDS             3072..3938
                     /locus_tag="LNE23_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6932319.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            3935..4783
                     /locus_tag="LNE23_002507"
     CDS             3935..4783
                     /locus_tag="LNE23_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6932320.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            4797..6269
                     /locus_tag="LNE23_002508"
     CDS             4797..6269
                     /locus_tag="LNE23_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBK6932321.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWINDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMTAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKGLVYDQHYLRLEINLKQATKLELHYAKGPHHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6286..7158
                     /locus_tag="LNE23_002509"
     CDS             6286..7158
                     /locus_tag="LNE23_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6932322.1"
                     /translation="MIYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(7392..7757)
                     /locus_tag="LNE23_002510"
                     /pseudo
     CDS             complement(7392..7757)
                     /locus_tag="LNE23_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2380 a         1350 c         1672 g         2355 t
ORIGIN      
        1 ttaattttgt tggcaaatat agtttccgct atgaaaaaga attatcgaat ggaatactac
       61 gtccgttaaa tatagagaat tattgagaat gttcgtcttt tggttcctac cgtgcatttt
      121 cgcacggatg ttccagatac tatttttgaa tgtataaaat tgaaaacaca agaatcctat
      181 taaatcaacg attcttgtgt ttttaatttt tcactaccgt gcattttcgt ttctatgttc
      241 catgtacccc aatcttgaaa aattaattaa agacttatat aaagccattg acgacttagt
      301 cgtggtgcta ccggagtatc aatgtttaac cagtatccca ggtattggtc ctgtttaggc
      361 tgctggaata ctcgctgaaa ttggtccaat tgaacgtttt tctgatgaaa taaaattgac
      421 aaagtatgcc ggcctatatt ggcgacagag tcaatccggt aaatctgaat acgaaaattt
      481 ttttacgaaa agcttcacgt ctaatatcta ttttaaaata aaaatattcc tgctaatttg
      541 gaatcgtttc cttcttttgg tataataata cctgaaattc ttttgagagg agttgttcgt
      601 gaagtgaaaa ctactatgaa ggatgttgct gcgttagcag gagttggtgt gggtacggta
      661 tctagagtaa taaatggagt aaaggtgaaa gaggttacca aagaaaaagt aatggctgca
      721 attagtgagc ttaattatga gcctgatgaa tatgccagag gtatgaaaac gaatagcagc
      781 aatacaatag ctcttatttt acctacgatt tggcatccct tcttttccga gtttgcctac
      841 tatgtagagg aagctctcag ccaaaaaaat tataagcttt ttttgtgtaa tgcgaacggc
      901 agtgcggaaa aagaaaaaga atatatccaa atggtcaagc agagtaaagt agacggaatt
      961 ataggtatta cctattcaga cattgataaa tatgtatcat caaatttacc atttgtcagt
     1021 atagaccgcc atttttcgga acaggtttct tatgtaacgg cagataatta tttaggtggg
     1081 caaattgccg ctcaagaact acttactcgt aactgtaaaa acttgtgcta tgttgggggt
     1141 gtttcacctt accctaacga aacaaacaat cgtaaagctg gtttttacaa tatttgtgag
     1201 gaaaataaaa tagctgtgaa ggtattggat atgcctgaac caattagcaa tttggaggaa
     1261 caattgctcg cttttttttc ggataatcct gaaattgata gcatttttac catcaatgat
     1321 ttcatggctt tggatgtgat aaaagcccaa aaaaaactag ggaaggaagc acctaaggat
     1381 tttcaaatta ttggttttga tggagtcaag ctatcgttgc aaaaagacta tttactatca
     1441 acgattgtac agccgataga ggaaatggcc aaggtttcag tagaaacact catggcgatg
     1501 attttaaatg aagaaccgcc taagcgtgtg gtagtaccgg ttttctttca cgctggagaa
     1561 acgactaaag agtaaaaaac cggtttataa cgattttttt tgagataggt tgacagaaag
     1621 tggtttcaat gatatcttaa aaccaattaa ttggaaacgt taccaattaa ttggaaaata
     1681 aaacggaaac gtttcaaaaa ataggaggaa catcgatgaa aaggagaatg ttagcaattg
     1741 cagcaatttt agcattaaca gctggaggac ttgcaggatg tggcagtaag gagaaaagtt
     1801 caggcagcag tgattcagga gaaattaaga tttgggttca gttttctgat gaaacgcctg
     1861 aaggaaaagc ttgggcagag attgtgaaca attttaatga tgaatacgag ggtgaatata
     1921 aagctgttac tgagtatatt cctcgtagcg gaagcggcgg tggatatgag gataaagtaa
     1981 atgctgcaat tacaaccaat acccttcctg atgttattac gttagatgga ccgaatactg
     2041 cagcttatgc tcattcagga gtcattgctc cattagatga ttatttgaaa gatgcggaca
     2101 tggatgatgt attagacagc attattcagc aaggaaccta cgatggcaaa atgtatgcgt
     2161 ttggtttctc agagtccagt gtgggtgtct attacaataa gtcgttgttc aaagatgctg
     2221 gaattacaga agatcaattg ccgacgttag aagatccttg gacttggacg gaatttcaag
     2281 caatttgcca aaagctgact gataaataca aaacgcctgc aattgatatg caaatggcgt
     2341 cgggagacga aatgctgacc tacgcatata caccatttgt atggtcgaat ggcggcgatg
     2401 tcgttaatgc tgatggaacc aaagccgaag actattttaa cgataaaaaa tccgttgaag
     2461 cgttggaatt tattcaagaa ttggttgaaa aaggttacac cacaatcagt cctattgaaa
     2521 aaggatttga aactggcaaa tatgccatgc tgctgagcgg ttcttggaca atggcagatt
     2581 tagaggaaaa ctatcaagag attgattatg gtattttgcc ttaccccgtt tcagatgcaa
     2641 ctggggagtt agtttcacca accggcagct ggcagttagc aatgactgag aaaacagaga
     2701 aaaaggatgc ttctgcggcc tttattaagt ttgcaaccag tacagaatct agtcgtatta
     2761 ttagtttagg gaacagtgtt ctacctatca gaaattcaac agttgattta attaaagatg
     2821 aagtgtcgga aggaatgcag gtattaatta agcaaaacca aatttctgga catgcccgcc
     2881 cagtggttgt agcttatccg caagtatcta gagcattcca acaagcagtt caagatatta
     2941 gctacttcga ggaaaaccca gatgtacaaa aagtagcaga tagtcgcgct gctgaaatgc
     3001 aacaggcaat tgatacttca ctaaaataga agtttctaaa gggggccaat caagtgttaa
     3061 aaaaaccgac aatgaaagac aatttgatag gttacggctt tctagcgcca gctttgctgc
     3121 tgctgacgat ttttctgatc atacccgtaa tcatggtaat ttattatgcc tttactgatt
     3181 attatttgtt aacgccagat gctagacaat ttatcggctt agaaaatttt aagcgactgt
     3241 tcgctgatcc aatttttgta aaaagtattt ggaacacggc taaatttgtt gtttggatta
     3301 ttccggtcca attaggggca gctttaggaa tggcacttat tatcaacaaa aatcgtaaag
     3361 gaaatatctt ttttaaagta gccttttttg cgccagtcgt gatgtcctta gtcgttattt
     3421 cgattttatg gttgtacttg ttaaatccta atgaaggtct ggttaatacg ctatttgcaa
     3481 agattggaat tgcttcgcag ccttttttga ctagtcccaa acaagcaatg tatacgcttg
     3541 ttttcgtatc ggcatggcaa ggtgcaggat atcagatgct gctgttttta gggggaatgc
     3601 aaaatattcc gcaagacgtt tatgaggcag ccgaaattga tggtttttct aagtttcagc
     3661 aatttcgcta tatcaccatg ccattattaa agcccacggc gatttttatt ttacttacca
     3721 cattgatttc ggcctttaaa ctaatcattc agccaatggt aatgactcaa ggtggaccaa
     3781 tgaattctac tatgacaatg gtttattaca tttaccaaac tggatttaca gaccgtatgg
     3841 taggctactc cagctccatt gcgttaatct ttaccaccat gattggttta attacaattg
     3901 ctcaaagaaa attggtaaag gaggatgatc cagcatgacc aagaaatttg gaccgctaat
     3961 gatcgtagaa tattgtttgc tgttcctttt agcggcatta ttcattttcc caatgctgtg
     4021 gatggtagtt tcctctatga agcctgaagc agatgtctat caaaatttag cttccttcaa
     4081 agctttttta cctagtttga atccagctaa ttggtttaaa acttatcaag aagttatttc
     4141 ccggttcagt gtgggaactt atttattaaa cagtattttt tatggtttaa cctttgcctg
     4201 tgggtcaatc attgtaaact ctttggcggg ttttgccttt gcgaaggtta atttttcagg
     4261 caagaaaatt atctttggag ctttgttagc tctgctgatt gtcccgatgg agacggtttt
     4321 aattccgcaa tttaccatcg ttaattcact aggcttagtg aatactcgct tggcagtgat
     4381 tttaccagcg atggccagcg cgtttaatat ctatttgttt agaaatttct ttatcgcgat
     4441 tccagaagaa atcattgaat cagcgaaatt agatggtgca aatattgtta aaattttctt
     4501 tcacatcatg ctgccaatgt ctaaaccagc catagccaca gtaggtgtct tatcctttat
     4561 ctccagctgg aatgattata tttggccgtt aatggtctta actgacagca gcaaattttc
     4621 aatgcaagtg gcaattacta cgatcaatac tacacagcca gtttatatca atcaagtgat
     4681 ggctgtttta accattgcaa cgattccact gattattgtt tatatcgttg cacaaaaata
     4741 tattctgcag ggacttggcg gttctggaac gggtataaaa taggagtgaa tagtgaatgt
     4801 tgcaaaaact tttaaaggcc aatgagttta ttgagaaaaa tgctcaaaca gtcaatcaag
     4861 aataccggaa taaatttcat ttgatggctc cgattggctg gatcaatgat ccaaatggtt
     4921 ttgtttatta tcaagaggag tatcacttgt tttatcaata ttatccttac gatagcatct
     4981 ggggaccaat gcattggggc catgctaaaa gcaaggatct aattcactgg gagcatttgc
     5041 cagtagcact ggcaccaggt gaaagctatg acaaggaagg ctgtttttcc ggcagtgcca
     5101 tagaaaagga tggcaagctg tatttgattt atacaggaca caatgttgtg gaagggcaag
     5161 ttcgacaagt tcagtgtttg gcagtttcag aagatggcat tcactttgaa aaatatgccg
     5221 gaaatccaat catcgcagaa gatcacttag cttctgttgc aacgaccgaa gatttccgcg
     5281 atcccaaagt gtttcagcga aaagaagact attttactgt agtcgctact aaaactgcag
     5341 atgctcgtgg acaaattgtg atgttccatt ccaaagattt actaacttgg gagttttatt
     5401 ccgtattgct ggagggaact ccagaacaag gcattgtatg ggaatgccca gacttgtttg
     5461 aactagatgg caaagaagtc ttgattcttt ctcctattca aatgactcca caaggcaatg
     5521 cttaccaaaa tacgaactca actgttgctt tcattggtac tgtcgattgg caaacaggca
     5581 aatttctagt agagaatttt catgaaatcg atggcggttt agatttttat gccccccaga
     5641 cctgtgaaaa cgaagcaggc caaagaataa tgactgcttg gatgcaaatg tggcagcgta
     5701 aaatacctac gaatgatctg ggccatggtt ggtcaggttc catgaccttg cctagagagc
     5761 tgagggtggc tgataatcaa ttgttgcaac ggccagtttc atcaatttat caagtaattg
     5821 atccgcagga aaggattgaa tgggcgaaga ctgaagagaa tcgattctta ctgaagggct
     5881 tagtatatga ccagcactat ttacgcttgg aaatcaattt aaaacaggca acgaaactag
     5941 aacttcacta tgcgaaggga ccccaccata cattactgct taactatgat gttgaacggg
     6001 agtgctttac tgcttccagg gaacacatgc cgtatgattt atcaggagct gaaaaggaag
     6061 tgttgaaaaa gcgcagtgtg acgattcctt tggaaaataa ttgtctaacc atcgaaattt
     6121 tccgtgatac ttcagctatt gagctttttc taaatgatcg tctgactatg agtacaactt
     6181 tttatgagac agaaaaagga caggattttc ttttgctggc agacagtgaa gtaacctttt
     6241 cattggaaag cggcttaatt aatctataga agggagagct cagtgatgat ttatggtgca
     6301 attgaagcag gcggcacaaa atttgtgtgt gccattggcg atgaagaaat gacgatcaag
     6361 gaaagagtga gctttccgac aacgacacct gaggaaacaa tgcctttagt cattgacttc
     6421 ttcaaacaat accaagcaga tttagcaggg attgggattg gctcatttgg gcctatcgat
     6481 atccatcgcg attccgccac ttatgggtac attacctcga ccccgaaatt ggcatggcaa
     6541 aattttgatt tcatcggtac catgaaaaaa gaatttccga tcccgatttc ttggacaaca
     6601 gatgtcaatg cggctgccta tggtgagtat gtcttcggca gtggcaaagg attgagcagt
     6661 gtcgtttact atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt
     6721 atcgaagggt ttagtcatcc agaaatgggg catatgctcg tcgtccctca tccaaaagat
     6781 gactttgctg gcagctgtcc atttcatggc aactgtttag aagggatggc cgctggaccc
     6841 gccatcgaaa aacgcttagg gaaaaaagga caagaggttg cagaagatga tccttattgg
     6901 gaaatcgaag cctcctatat tgctcaatgt gcctacaata caaccttgat gttttctcca
     6961 gatgtgatca tttttggcgg tggtgtgatg aaacaacgcc atatgctgca aaacgtccac
     7021 gacgcatttg caaaattggt caatgggtat gtcgaaacac ctgagttaag caaatatatc
     7081 gtgaccccag cattagaaga caacgctgga acgctcggtt gtttggcatt ggctcgagaa
     7141 gcggtcttac attcataaac gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga
     7201 tacaactgaa aatttagtca taaccagtta ggcagagtgc ttaactggtt tttgttttac
     7261 ttggaagatc aagccttgaa tgagataaat tcttgctcga aagttatgga aatttcgata
     7321 gccgtaggct acttgtattg tgcaatagtt ttttacaaaa aaaaggggtg cggatatttt
     7381 tttggacctt tttaacaaat atatctttgc ttcttccgat attgaagagg gcttagccct
     7441 cctaaagata acttaattcg ttgattatta taccaatgaa tataattatc caatttgttt
     7501 ataaactgat tgattgttgt attcttccag tcacgttgat aaaacatctc gttttttaat
     7561 cgtccaaaga aaccttcgca cgctgaatta tctggtgaat aacccttttt agacatagag
     7621 tgaagcaagt tgtctttgtt ccaatagtcc aactaacaat agcaccatcg taacaatcga
     7681 caagtggtga taagtagatt ttccctgcgg gaattctaaa ttcagtaata tctgttaacc
     7741 atttttcatt tggcttc
//
LOCUS       DAFCNS010000100         7673 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFCNS010000100 DAFCNS010000000
VERSION     DAFCNS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.103"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..519
                     /locus_tag="LNE23_002511"
     CDS             <1..519
                     /locus_tag="LNE23_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932323.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESSTTVSDANILANIILDFIFEHDIPFKNGYEILPQNEQYFFYKCLTNRVCCSCGKK
                     NADIHHVDSVGMGNNRKKINNSGRRFMALCRECHTKIHVEGFTTFTTKRKLTAVVLKD
                     SDLKRLGLNIIE"
     gene            527..1042
                     /locus_tag="LNE23_002512"
     CDS             527..1042
                     /locus_tag="LNE23_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK6932324.1"
                     /translation="MDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIMKPSDN
                     GKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVENLEWLS
                     SDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRHTVLNI
                     ASGKTFSFLED"
     gene            1147..1977
                     /locus_tag="LNE23_002513"
     CDS             1147..1977
                     /locus_tag="LNE23_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBK6932325.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1994..2845
                     /locus_tag="LNE23_002514"
     CDS             1994..2845
                     /locus_tag="LNE23_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6932326.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2842..3201
                     /locus_tag="LNE23_002515"
     CDS             2842..3201
                     /locus_tag="LNE23_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBK6932327.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            3216..3377
                     /locus_tag="LNE23_002516"
     CDS             3216..3377
                     /locus_tag="LNE23_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK6932328.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            3374..3679
                     /locus_tag="LNE23_002517"
     CDS             3374..3679
                     /locus_tag="LNE23_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932329.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            3679..3996
                     /locus_tag="LNE23_002518"
     CDS             3679..3996
                     /locus_tag="LNE23_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBK6932330.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVKKYKEILDKYME
                     DSK"
     gene            3993..4388
                     /locus_tag="LNE23_002519"
     CDS             3993..4388
                     /locus_tag="LNE23_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932331.1"
                     /translation="MILKYRAWNKKTQSFIDYGDLVLDLRSGKIYAGDIGLVESTIDV
                     TDQIELMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLGH
                     RNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            4385..4723
                     /locus_tag="LNE23_002520"
     CDS             4385..4723
                     /locus_tag="LNE23_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932332.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            4720..5088
                     /locus_tag="LNE23_002521"
     CDS             4720..5088
                     /locus_tag="LNE23_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932333.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            5085..5390
                     /locus_tag="LNE23_002522"
     CDS             5085..5390
                     /locus_tag="LNE23_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932334.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            5414..5617
                     /locus_tag="LNE23_002523"
     CDS             5414..5617
                     /locus_tag="LNE23_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBK6932335.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            5614..5910
                     /locus_tag="LNE23_002524"
     CDS             5614..5910
                     /locus_tag="LNE23_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932336.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDIELINDK"
     gene            5986..6402
                     /locus_tag="LNE23_002525"
     CDS             5986..6402
                     /locus_tag="LNE23_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBK6932337.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLIQYR"
     gene            7118..7285
                     /locus_tag="LNE23_002526"
     CDS             7118..7285
                     /locus_tag="LNE23_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932338.1"
                     /translation="MYRPQYLEQKYEVITVQNGNSEIVRKYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            7311..7655
                     /locus_tag="LNE23_002527"
     CDS             7311..7655
                     /locus_tag="LNE23_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK6932339.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         2827 a         1144 c         1736 g         1966 t
ORIGIN      
        1 gtacagcaac gaaacttcat ctatgcgctg ataggcgata ttttcatcga tacaggcatg
       61 ccaacggact tctggaagga attcttctac ttccgtttcg aaggtgtcac agggcgcaaa
      121 ataagcctca aagatgaatc gagcacaacc gtaagtgatg ccaatatctt agcgaatatc
      181 atcctagatt tcatctttga gcatgatatt ccgtttaaaa atggatatga aattttgccc
      241 caaaacgaac agtatttctt ttacaaatgc ctcacgaaca gggtgtgctg tagttgcgga
      301 aagaaaaatg ctgatataca tcacgtagac tctgtcggca tgggaaataa ccgaaaaaaa
      361 ataaacaatt ctggtaggcg ctttatggct ctgtgcagag aatgccatac gaaaattcat
      421 gtcgaaggtt ttactacatt tacaactaaa agaaagctta cggcagttgt tctcaaagac
      481 agtgatttaa aaagattggg gttgaatatc attgaatgaa ttatggatgg atatcgaagg
      541 atacgaggga gtatatcaag ttagtagctt aggaagagtt aaatctaatc gccctaacta
      601 tgtaattaat aagggatacg ttaacaaaga aggatttatt atgaaaccat ctgacaatgg
      661 gaaagggtat caaattatct tcttaagcgg caaaggtttt agagatagaa ggtatgtcca
      721 tcggctagta gcttttcact ttttaaaaga agcgtatttc gaaggtgctg aagtcaatca
      781 taagaacgga gataaatcta acaatactgt tgaaaaccta gaatggctca gttctgatga
      841 taataaaaag catgctagaa aaaatggatt aactaacttg aaaggaccat caaaactaac
      901 tgatttgcaa gctttagcta tcaagcgttt atatacaaac aaattgatga gctccggaga
      961 aataatgaaa ctttttaatg tcagcaggca taccgtgctg aatatagctt cagggaagac
     1021 atttagtttc ttggaagatt aagagaaaca caaaataggt gtgactaatt tcaaaaataa
     1081 atatcaaatc aaagggatca agttaaacca ggaaacaatt aaaaagttaa ggataggagg
     1141 ataaatttgg ctgagataag ttggatcaaa cttaaaacta ctatgtttga cgatgaaaaa
     1201 atacgattaa tccaagctgt tcctgagtcg gatgccatca tcgttatatg gattcgatta
     1261 ctagttttag caggaaagac taacgacgat ggtctgatat atatccagag gaacatgcct
     1321 tataccgaag aaatgcttgc tacattgttt ggcaaaaacg taaatacggt tcgcttagcg
     1381 ctaactacat tggcaaattt caacatgatt gatctaagca gtgatggact aattgccatc
     1441 agtaattggg aaaaacatca aaatatcgag ggtatggata aagtaaggct aaaaaatgct
     1501 gaaagaaacc gtaaatacag ggaaagaaag agacaggaac gtctcaaatt ggaaaatgac
     1561 gttagcgtga cgtcacgtga cggtacagat aaagatatag aagaagataa agatatagat
     1621 aaagaagaaa agaaaggtaa gtattctgac gaacacttac gccttgctaa aaagttgcaa
     1681 agtaatttaa ctgaagattt tccaaaagaa atgaacaaag tagatatcga aaaatgggca
     1741 gacacaatca ggttgatgga agaaagagat aaagcatcta tagaagcgat tgagtatgtg
     1801 atcaattggc tacctacaaa tgaattttgg tttggaaata ttagaagtgc taagaaattg
     1861 agagaaaaat ttgagaagct caaattcgaa atcaaagcag acaagaagaa tcataaaaag
     1921 caaagtcaaa aactacagta cagcaatcct agtgaatatg acgacttgcc aatttaaaaa
     1981 ggagatgcat cacatggaaa gcctagcaaa tgctatggag aaactaataa gaagagtatt
     2041 agtgcaaagc ggaaaatgtc cagaatgtag cgaacctttg tatagttggc gagctaaaaa
     2101 taaggatggt tcagaacgtt gtaaaccaac atgcatgagt tgtggttata aagcgttacg
     2161 tgtgaaagag gatatacaga ccgaacggat atataacgat agcttaaaag cacgagcgtt
     2221 gagttttttt caaaatggtt cggtattaac agataaaact ttgtttaaat gcaaaatgga
     2281 aaattatcac gtagtggatc aagaaacaaa aatcgcttta gaaaaagcaa aaagctatac
     2341 gaatgaggtt ctgctgaacc atcctgcaca tttcattcta tcagggaaat caggaagcgg
     2401 aaaaagccac ttatcaatgg ccacagcttg ggaaatactt gagcgctcaa attatgacaa
     2461 gaaaatactt tttataagct atcaagaatt attagagcaa ataaagtttt cttataacaa
     2521 tactgaactg agaaaagaaa ttgaaggatc gcttatagcc gatattaaaa caactgattt
     2581 ggtggttttt gacgatattg gagctgaatt aggtagtggg gtatcaaata gtaggcagtt
     2641 tacaaacaac acgttaaaca cgctcttaga agccagacag aacaaggcaa cgatcatcac
     2701 aacaaactta tctggtcctg aactaagaga agcctacggt gaaagaattg tttctaggat
     2761 atttaagaat tcagaaggtt atgcgctgaa attccaacaa acagcagaca agcgcataaa
     2821 accagtgaaa ggtagtatcg catgaataaa taccgtaata aaaaaactgt tcatcgaggt
     2881 atcaagtttg attctatcgc agaagcagag tattacgatc tagccttgtg gcaagctgaa
     2941 gcaaatggct ggaaagtaaa acttcaggaa agatttgagc tgatgccgaa atttgaacta
     3001 gacggaaaga agtatcgcaa gatcgagtat attcccgact tcacatttta taaaaacggc
     3061 aaacttgtca aagtcgtaga tgtcaaagga atgcagacaa aagactttaa gatcaaggca
     3121 aagttgttct gtcatcaata tcaagcgccg ttgatattag ctaaaaaata tcggaatacg
     3181 ttcaaggaag agcgttttta acgaggtggt ccatcatgac aacagaagaa gtgattcaaa
     3241 tgcgtattcg aaacattcag cgtgaaattg acgatctaga acgaacaaag gcagtgatgg
     3301 tcaatgaaac ggcgagaaag gcaatcgatt tgcacataga gaacttaaga agggaaattc
     3361 gtagattgga ggaatgagcg tggataagaa agcgacaatg aaacgaattg ctgaattaac
     3421 caagtcagaa tcttggcagg aagacaaaga aatagttgca gaagtccaaa agctcggtaa
     3481 atcaatgtgg actgaaaagc ctaaacggaa aacgccgaga aaaattgcaa tctggcatgg
     3541 tgatcgaatt ctagtaacag gtacagctga acagttatct gaaattactg gattaagcaa
     3601 aaacattatc tgggatagag ctaggagctt atggattgat tcaaaaggac gacagtttag
     3661 gtatgtggag gagagataat ggatctcatt acacaataca gtgacatcat cctcaagaaa
     3721 atcatgatga agattcagaa agacaaaaaa tcaaaagaac gagcggaatt agttaagttg
     3781 gaaatggctg aaacaggagc aggagtgcga agtagcaggc attggaaagc agcagcaaac
     3841 attgaatttt attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg
     3901 caaacaaatt ggagcaagaa acttcatcaa gatcgtttca agtttgtaaa gaaatataaa
     3961 gagatattag acaaatacat ggaggacagc aaatgatact gaagtacagg gcatggaata
     4021 agaaaactca gtcatttatt gattatggtg atttggtttt agatttgaga agcggaaaaa
     4081 tttacgctgg ggacattggg ttagtggaaa gcaccattga tgtgactgac caaattgaac
     4141 tcatgcaatc cacaggaatg aaagataaga atggtgtgga gatatttgaa ggggatgtag
     4201 taattacatc aaaaggagcg attggatatg ttgcgtatct tcaacaagaa gccggctttg
     4261 tagttgtact aaagaaaagt gattatagat taggccatag gaatactggc gaaagttatg
     4321 atgtagcaac tgctcatgaa gtcatcggaa atatatacga gaatagcgag ttattggagg
     4381 tagagtgatt atcttagcta ctgactacat cgattcttta aaagatgaaa atgggaatgt
     4441 cccagcattg ctaaattgtg gaattaaaag caagaataga atcattttgt tacggatatc
     4501 ggctgattta gcaaaatatc tttatctgag caagacagca gtatattaca ctgcggctac
     4561 acgttatcag tacaagggaa aaacaatcag tcaggattat tatgatcgtt taatcagtcc
     4621 tgacatgcat ggaaaatcaa aatcagcgat caagttattt ggcgcaatag agatatatgc
     4681 cgatgatttt ccaaatttat ggttgaagga ggaagcggaa tgaacgagga aataaaaaaa
     4741 tctattcgtt acattgatag agcgttatcg caactcgaac ccttgcgaat tgaaagtcat
     4801 aaaggaatga gtgtctaccc aaatcagtat gacaaaatga acgctgattt acgtaatgca
     4861 aagctggcct taattaacat taaaaataac aaaggctgcc agtattgcaa acacagcgaa
     4921 ccgttgaatg ataccagcaa ctctaacttt gcaactgcaa ttgagtgtga ggaaaatgga
     4981 tactacattg caactgagta tgacgatggc gattatgcag ataatggcta ttctgcttat
     5041 atccaatatt gccctatgtg cggtcgaaaa ttggaggtag cggaatgaat gttcaagttt
     5101 atttaaaaag aaatttggcc aggtttcctc gaagctggga tacaaattcc cacccatccg
     5161 aggcagaaga agagagctat ctaatgtctg ctaacatgat agaaattaca agagatgatg
     5221 cggatgagtt tgataaaaaa agatcatggt actcggtttc gaatccaatg tatacggctg
     5281 taatagaaga ttatcatata tccgacagat ttattctggt tgatctagaa agacctaaga
     5341 aacgaattaa acagtataga cgttgcgggt tttcaacaaa gaaatggtga aaacaaaaca
     5401 gaggtgactc agaatgaaca acagacatcg cagaataaca aaactaagaa aacaggaact
     5461 gaatgtacta aagacaaagt ttgaaaaaga atacggagtt tctgcagaag aaacatataa
     5521 agtggcaagt cagtgtgttg ctgatgcaag tgatgctatt cgtaagtttg gaatttcgat
     5581 attaagtgat gatcgtaaat gggaggaaaa agaatgaaac taaaagacgg attttacgct
     5641 agtagccacg gtatcggcgg tttaatgctg gatatgcaga caaagaaccc taaaacacgt
     5701 aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg aagcagaagg gttcatctat
     5761 ccatttcgtg gatatgtaga acatgtctat aatcactcag cgatcattcg cattgaaaac
     5821 acgatggaat gcgacaagtt attagcgaaa agcaaagaga atttagcagt ggtgagattg
     5881 gtggatattg aactaatcaa tgacaaataa aaaagccgga tcgctccgac taacataata
     5941 aaacagacaa gtttattata tcacataaag gagcggtttg acttgatgca attgttacga
     6001 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
     6061 ttggagcgta tggcaggtcg ctctttgata gatattaagt cgccgattat aacggatatg
     6121 ccgaaggcgc cgaagcacgg caataaggca gaggacgcga tcattcagat gatggatata
     6181 gaagcagaga gagatgcgat tctagcggct ttgatggctc ttagtctgat tagtcgtcag
     6241 atactctact acagcttctg cgacgtaaat aagcattcta attatgaaat aggtcaattg
     6301 atacgaggat acggagagaa gaatgtagag aagttgaaat ccatcgcatt gattgaattt
     6361 gctgaagcat ataaaaaagg cgtattaatt cagtatcgtt gattttgtag ggtttttgta
     6421 gggatagtgt agggtttttg agcggtttaa cgtgatatta tggtagtgtc gaaagattag
     6481 gaaacaggac ttcgacaaaa taaaatgtaa gggaggaaat ctccctcatc gtttaattaa
     6541 gcttcgatag acagcagcgg aaatattaag aataaggatg tgaattttaa ctccttctaa
     6601 attgttctta ttatctatca tccgttgctg tctattgtca ttatgtcact gtggcggaaa
     6661 gggtagacgc ttaaaaataa ggtcaatacg tcgagggata gccttaacgt tttatgattt
     6721 gaccatgcaa tgttcgattc attgccagtg acttaaggaa cctacggaaa caattcatct
     6781 tatcggatgc cgatgaattg gctgactagt cgggatgcta ctagcagtta gaaggcataa
     6841 aatactagcg cagacgtgcg ccactctcag gtgtaggtta ggagagaaac attagttggg
     6901 gttattagga atacgataac ctgcttgcga caaagctttg tactgtcgcg ttggtcatga
     6961 acagagacgg tattctgttt cagtattcgt tagcaaccga gggatgtggc agtggtgagg
     7021 tgcaggaagt atcagacttg tttgtgtgca agttactatt acatattaga tcactctttg
     7081 agtggtcttt ttattttgca caaaggaggt aacaacaatg tatagaccac aatacttaga
     7141 acagaagtat gaagtaatca ctgttcaaaa tggtaacagt gagatagtac gaaagtatag
     7201 aagaccaata aagagcgata catataaacg aaaggaaaac aatgaagtta ttccattgta
     7261 tggcaaaaga atagctaagc attaaataag attgcgaaag gagacgggac atgactgagg
     7321 aattctatag atggctatta cagttgataa gagaagatcg tttggttaag ttctatcagt
     7381 ctcctaaatg gcgcaggctt agagagaaag cgatgaaacg agatcactat gaatgccaag
     7441 agtgtagaag actaggtaag tatcatagag tagagaacgt tcatcatata aaggaagtca
     7501 aggatagacc tgacttagct ttagatttag ataatcttat ttgtttatgt gttgaacatc
     7561 ataatgaagt tcatggcaga tatcttacag cgttagataa acaagagaag aagatagaaa
     7621 gcttcgctaa cttcgatgca agtgaaaggt ggtaagtgca tgatcatcaa tga
//
LOCUS       DAFCNS010000101         7600 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFCNS010000101 DAFCNS010000000
VERSION     DAFCNS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.104"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(943..1356)
                     /locus_tag="LNE23_002528"
     CDS             complement(943..1356)
                     /locus_tag="LNE23_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBK6932340.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="LNE23_002529"
     CDS             complement(1812..2297)
                     /locus_tag="LNE23_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932341.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="LNE23_002530"
     CDS             complement(2436..2651)
                     /locus_tag="LNE23_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBK6932342.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="LNE23_002531"
     CDS             2875..3675
                     /locus_tag="LNE23_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6932343.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="LNE23_002532"
     CDS             3694..5562
                     /locus_tag="LNE23_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK6932344.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGHYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="LNE23_002533"
     CDS             5555..6046
                     /locus_tag="LNE23_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBK6932345.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="LNE23_002534"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="LNE23_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBK6932346.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="LNE23_002535"
     CDS             6606..>7600
                     /locus_tag="LNE23_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBK6932347.1"
                     /translation="MYGGIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1444 g         2236 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggccatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtggtatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAFCNS010000102         7584 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFCNS010000102 DAFCNS010000000
VERSION     DAFCNS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.105"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(32..1180)
                     /gene="nagA"
                     /locus_tag="LNE23_002536"
     CDS             complement(32..1180)
                     /gene="nagA"
                     /locus_tag="LNE23_002536"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBK6932348.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1256..2092)
                     /locus_tag="LNE23_002537"
     CDS             complement(1256..2092)
                     /locus_tag="LNE23_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6932349.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSQDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2276..3493)
                     /locus_tag="LNE23_002538"
     CDS             complement(2276..3493)
                     /locus_tag="LNE23_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6932350.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     ITSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3642..3821)
                     /locus_tag="LNE23_002539"
     CDS             complement(3642..3821)
                     /locus_tag="LNE23_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932351.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3876..3959
                     /locus_tag="LNE23_002540"
     tRNA            3876..3959
                     /locus_tag="LNE23_002540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3910..3912,aa:Leu,seq:gag)
     gene            complement(4199..5569)
                     /locus_tag="LNE23_002541"
     CDS             complement(4199..5569)
                     /locus_tag="LNE23_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932352.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSLQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(6284..6574)
                     /locus_tag="LNE23_002542"
     CDS             complement(6284..6574)
                     /locus_tag="LNE23_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932353.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(6782..6985)
                     /locus_tag="LNE23_002543"
     CDS             complement(6782..6985)
                     /locus_tag="LNE23_002543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932354.1"
                     /translation="MNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLE
                     KAKDEVEDTADKSQQIVEEHLRK"
     gene            complement(7082..7474)
                     /locus_tag="LNE23_002544"
     CDS             complement(7082..7474)
                     /locus_tag="LNE23_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBK6932355.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEIN"
BASE COUNT         2307 a         1508 c         1169 g         2600 t
ORIGIN      
        1 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
       61 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
      121 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
      181 tcatgactgc ttgttctggt gttgctaatc cccaatctac aacattttta attgcttctt
      241 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
      301 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
      361 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
      421 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
      481 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
      541 caaatacact ggcgccagct tcaacagctt tttgcgcttc ttctagcgta gcgttactgt
      601 gacctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
      661 gacgttccgg tgctaaagca atttttttga ttaaaccgcc tgaagcttct tgccattcat
      721 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
      781 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
      841 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
      901 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
      961 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
     1021 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
     1081 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
     1141 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
     1201 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
     1261 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
     1321 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
     1381 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
     1441 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
     1501 ttgatcgttc gtcagctgat tggtgcaaca gttgaatgct tcgactcaaa taatagcgtg
     1561 cttcagccgt ttttcctgca acaccatata tttttccaat gcaactgatc agttgtattt
     1621 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
     1681 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
     1741 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
     1801 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
     1861 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
     1921 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
     1981 gacttaatac actttgtgaa caaatatctt gcgaaagcat tttttgcgtc attttttgtt
     2041 cttttcgaat ctctttaatg atcgtgccaa aggactcgat taacgtttcc ataagtatcc
     2101 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
     2161 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
     2221 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
     2281 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
     2341 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
     2401 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
     2461 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
     2521 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
     2581 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
     2641 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
     2701 tatagccttt aacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
     2761 ctacacgatg agatttggat agaccattca gcgtaacaac gaacaaatca ggcgctaatg
     2821 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
     2881 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
     2941 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
     3001 tttttgatgt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
     3061 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
     3121 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
     3181 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
     3241 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
     3301 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
     3361 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
     3421 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
     3481 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
     3541 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
     3601 aaaacgtaag tattaaaaaa acacaaacaa gcgttaagaa cttaattgtc atgatagtca
     3661 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
     3721 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
     3781 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
     3841 gtaaaacctc gctatcatta ttgtgttatt attacgcaga tgtgttggaa ttggcagaca
     3901 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
     3961 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
     4021 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
     4081 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
     4141 taatcgaaaa acgagtaaaa tccctcacct aatactagag cgagggattt tttgtatatt
     4201 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
     4261 taaattcatt tttttgtaaa ctcatgcttt tcattagctc tttaactgcc attagtacta
     4321 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
     4381 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
     4441 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
     4501 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
     4561 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
     4621 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
     4681 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
     4741 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa
     4801 atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc
     4861 atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta
     4921 attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt ccattgacaa
     4981 tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact tgttcttgat
     5041 tagttaaaaa tatcaataaa ggaatactta cccaatatat ttttctattt atttcttctc
     5101 ctcttaagag ttgaaaaaac ggataaaaag ttagtaaact aaatgctact ggccactgat
     5161 agttcaaggt ggttgctctc caaccggttt cccccattac tgtcaatggt attaaacagt
     5221 atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca tacttgcaaa
     5281 gtaatactac aattcctaac ataatcgtag aatttaataa tttccatagc ataaagtgtt
     5341 tagaaaagaa cattaagaat agttctataa taattcttga actccatgtc ttataatgtt
     5401 ctatcgaagc acccaaatag ccaagttcat ctaatctact tgcataaact acgtcatccg
     5461 ctctcatagt cggcatatac atattaaaga gtaaaaagat accccaaatc cctattagta
     5521 acaagatcag ttctttgtta ttcgttattt ttctattatg tttgttcatt tttatactcc
     5581 ttcttatatt tcctaataga gtataacata gctttaagca ttacttaaag ataacttaaa
     5641 agttatcttt taaatactta attgttaaat accttatttg atgcaaaaga aatctacgat
     5701 agtcaacaaa aaaaattttc attatatcca gaaaaatttt tcttagttaa ggacctatgg
     5761 attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca tattgctttt
     5821 aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt aggtttaact
     5881 gtcgaaccag gacgttctcg tgtgaaagac gaggcttctt caatttactt ttacgactat
     5941 gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa atcctacaat
     6001 tccactacct gagactagta atgactagtc tctttttatg gcctctgatc cccccattga
     6061 gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat ccctcaaatg
     6121 agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta tatatagatt
     6181 gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgaa tccatctttc
     6241 caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt attctttcat
     6301 cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca attttgggtt
     6361 tccctaacac atgataggat ctcacaacaa attgctttct attattataa taacctgtaa
     6421 cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg gaagcaatca
     6481 aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact tgcaaagtac
     6541 ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata ccattatacg
     6601 aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt tttgtctatt
     6661 acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta ctaaaaaaaa
     6721 gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt gttaagaaag
     6781 attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta tcttccactt
     6841 catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt tcagcagttg
     6901 cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt ttttcttgtg
     6961 tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg
     7021 cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt
     7081 tttagttgat ctcatctagt ttgacattga tttcattttg cttgctttgt agttgtgcat
     7141 ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt
     7201 tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg
     7261 tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct ttatctgtca
     7321 atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc
     7381 acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa
     7441 cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa tccaatactt
     7501 ttacatacta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa ttacatatgt
     7561 aattttaata taaatactat caaa
//
LOCUS       DAFCNS010000103         7578 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFCNS010000103 DAFCNS010000000
VERSION     DAFCNS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.106"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(73..1176)
                     /locus_tag="LNE23_002545"
     CDS             complement(73..1176)
                     /locus_tag="LNE23_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBK6932356.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1266..2222)
                     /locus_tag="LNE23_002546"
     CDS             complement(1266..2222)
                     /locus_tag="LNE23_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBK6932357.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2503..4437
                     /locus_tag="LNE23_002547"
     CDS             2503..4437
                     /locus_tag="LNE23_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6932358.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            4894..5310
                     /locus_tag="LNE23_002548"
     CDS             4894..5310
                     /locus_tag="LNE23_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBK6932359.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5324..5890
                     /locus_tag="LNE23_002549"
     CDS             5324..5890
                     /locus_tag="LNE23_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBK6932360.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            5913..6899
                     /locus_tag="LNE23_002550"
     CDS             5913..6899
                     /locus_tag="LNE23_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK6932361.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7054..>7578)
                     /gene="scm"
                     /locus_tag="LNE23_002551"
     CDS             complement(7054..>7578)
                     /gene="scm"
                     /locus_tag="LNE23_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBK6932362.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2150 a         1453 c         1572 g         2403 t
ORIGIN      
        1 aaatacacta gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat
       61 ggttcgtact ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga
      121 taatttgaag gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa
      181 gatcgcttcc gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc
      241 cattgaaaag actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt
      301 gacacttgcg atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta
      361 ttttttgcca tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt
      421 ccgttcttta atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac
      481 tgtccagtct tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa
      541 gtcaacgatg gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat
      601 gtaacctttg gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt
      661 tttttctttg tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat
      721 ttcttcttgg gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc
      781 accaatcgcc atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac
      841 taacgtgtag acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc
      901 atcgatctca gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc
      961 gaaggcaggt ttcaatgctg attctttgcc ttcatcatac acacggatac gaacgtaagt
     1021 ccctaacaag aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact
     1081 ttcagtggtt gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc
     1141 cagtgtgatt aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa
     1201 ggtgagacga ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta
     1261 tgtgattatt tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca
     1321 gctgcattca ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct
     1381 gtaacaactt ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg
     1441 tcgttcagag tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca
     1501 ttatattctg tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat
     1561 tcagttactt tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag
     1621 ttcttttctt caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt
     1681 gtatctgtgt taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt
     1741 tcagatgagt gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag
     1801 aattggtcat tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg
     1861 tttttgttgt attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat
     1921 tttgattcag tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt
     1981 gaataatttt tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact
     2041 tttgcagatg attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa
     2101 gaagagtttg agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat
     2161 gctaatacgg caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc
     2221 atttctagtc cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata
     2281 agtcaactat ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt
     2341 tcgtgaaaaa acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaaaaga
     2401 acagaacatc tatataatat atggagattg tgtaataagt atcaaactaa aagaattatc
     2461 atatattcat tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt
     2521 gtcgttgtag gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg
     2581 aaaaaagaag atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc
     2641 gagttgcatg aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag
     2701 catttgtttg ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat
     2761 aaagaacgaa aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt
     2821 ggaatgggcg gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc
     2881 ctttggtcat ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa
     2941 gctgcattgg aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt
     3001 tctggattta ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc
     3061 aaagattata aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg
     3121 atcttgaata tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac
     3181 aacgtggaat tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg
     3241 aaagacggac gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact
     3301 tctgacgctg ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa
     3361 tatatgcagg caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt
     3421 tatgaagaat tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga
     3481 cacacagccg cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa
     3541 ggaaactatc aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt
     3601 gataagatcc atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa
     3661 tacttctttg atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc
     3721 catatcaaag atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta
     3781 tggagtgtgc cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa
     3841 atcattggga atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc
     3901 actggaaaca gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt
     3961 gcttctgctg ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa
     4021 gcagcgactc aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct
     4081 gtgattgaga agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat
     4141 gatgtagcaa tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca
     4201 ttgattgctg gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg
     4261 ttctgcttat caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca
     4321 ttgatgaacg gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa
     4381 cgtacactag gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata
     4441 gataaagaac gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac
     4501 agcctccttt ttatgtaaaa ttaaaggaat aagatgcccg agataagcct ccatgagcaa
     4561 gacacaggtg tcacaacctc tgttacaacc tcttgttata aacggtgttc cagaagtagc
     4621 ttcttcgaaa aataagccga aatttcacaa aaacttgaaa agtaattttc gtaaattcct
     4681 tcttatttct tgaagttgcc cacttctgtc acaacctctt gttataaacg gtgttccacc
     4741 tgctgcttct cggtaataag cccaaagaat tagaaaacat ggaaagctgt tttctattct
     4801 tcgttcttat tactcaaagc aagacacagg tgtcacaacc tcttgttttt atgctaaaat
     4861 tgggtgagtt aatctggaaa aaggagaaca aaaatgagcc ttaaagaatt tgctgaaaaa
     4921 agtctgctca aaccttggga tgtttttatt attttgcttt tgactgttct ttctttttta
     4981 ccagtcatga tttttggtct tcagcaaaac accgcaacag cagaaaaaga agcggtactg
     5041 cgtgtggatg gcacagaaat caaaacattc cctttgatgg ctggagaaga gagcaacaca
     5101 tatacttaca aagatgaaca cggagattat aacttgatcg agatagatgg cgatcgagta
     5161 cggataaaag aagcagattg cgatgaccag atctgcgtac gtcgaggctg ggcaacgaaa
     5221 aatggcgaaa ccatcgtctg tttgccacat aaattagtga tcgaagtccg ttcaacagat
     5281 ggaggtgacg aagacagtct gatttattag accttcacac accatgagta aattacaaaa
     5341 aatgatttat atttcgctac ttgttgcaca aggagtgatc attggtttat tggaaaatat
     5401 gatcccttat cctttttctt ttgcgcctgg tgcaaaacta ggcttagcca atctaatcac
     5461 gatcgttgcc atttttacca tgaaaaaaca agacagcttc ttactacttt ggctacgact
     5521 attcctgact actttgctag gcggaacgat ttccacattc ctttatagta tgagtggtgc
     5581 tttgcttagt tattttggaa tgttattggt caagcaatta ggaccaaaac gtgtcagtat
     5641 catcggaatt agtgcggccg gcggtttcat gcataatgtc ggacagcttt tgatggcttc
     5701 tttcattgca caatcatgga cagtcatgtt gtacttgcca gtcctttcag ttttagggat
     5761 tttatcaggg attgcaatcg ggatcgcagc gaattatctt ttacgacatg tgcagacact
     5821 tcaacgattc caaaaagatt atgagcaggg aaccaaacaa aaatggagcc caatgtttac
     5881 caaaaaataa tcgtcacaca aggagttttt ttatgaagct acatccaatg tgggaaaact
     5941 atcctgcttt aagtaaagaa ttgacagaaa cactaaaacg gatggaagaa actgtccgcc
     6001 tgaaaaacaa acctgtcgaa caagccatca aagaaacgat ccatgccggc ggaaagctgt
     6061 tgcgtcctgc ctatcagcta ttgttctcac aatttggacc agagcaagat cgagacaaag
     6121 ccatcgcttt ggcagcttct atcgaaatgc tccatacggc cacactgatc catgatgata
     6181 tcgttgatga agcggatctg cgacgtggtc agcctaatat ccgtagtcag tttggtaatg
     6241 tcgtttctgt ctatgcagga gactatttat ttgtctgctg ttttaaacta ttgtctgatt
     6301 actccacttc cttgaaaagt ctccaattga attctcgcag tatggaaaaa gtattgaatg
     6361 gcgaattagg gcagatggat gatcgctata attatgaact ttctgtaaaa gaatatttgg
     6421 aaaatatttc aggtaagaca gctgaactgt tccaattaag ttgttctgtt ggcgcttttg
     6481 aaagtggaac aagcgagcgc ttcgcaaaaa aagcaggaga tatcggactt tctatcggga
     6541 tggcctttca gattatcgat gatattttag attatacaaa agagagccag gaaatcggca
     6601 aacctgtact agaagacatg cgtcaagggg tctattcttt accgttgatc tactctttgc
     6661 aaaaaaacaa accaaagcta ctaccttatt tagagaagaa agcggcaatg acagaagaag
     6721 atgtcgacgc cgtacgcaaa atcgttgaac atacaggagg cgtggaagaa gctaggaagt
     6781 tagcggcctc ttacacagaa aaggctttga aggaaataaa gaaattacct gccacctctc
     6841 ttcgaacaaa agagaatctc ttttccctta cccaactgat tttggatcgc aaagactgaa
     6901 tagggaaagg ttcgatacgt aaataaattt ctcagaagga aatctacgtg ttttttgaca
     6961 aattgaattg tagttcttgt atttatgata aaaagcaaaa ataaagagca acgtccgtta
     7021 gcgacgttgc tcttttttat cttgaatatt tttttacctt ttcgatttct tataactcat
     7081 aaaagtgatt cttaaaccga ttacaagtaa tataacgcct gcgagtgtta tccaattatt
     7141 cgattgactt ccagttttag gaagtgctct ttttgatttg tttccattgt cattgcttgt
     7201 tttattagtt aagttaaagt tcgtcctgtt atccgaactt gtttttgaga aggagacact
     7261 tgcatgacgt gtatcgcttg tggtagacga cgtgtctgta ctatcgcttg aagttgatga
     7321 atctgagcta tcacttgtgg tagaggatgt atctgtactg tcacttgaag ctgacgtgtc
     7381 cgtgctatca cttgtagtgg tagatgaatc tgagctatca cttgtggtag aggtagtatc
     7441 tgtactgtca cttgaagctg acgtgtccgt gctatcactt gttgtggtag atgtatccgt
     7501 actgtcgctt gaagctgacg tgtccgtgct atcacttgtg gtagaggtag tatctgtact
     7561 gtcacttgaa gctgatgt
//
LOCUS       DAFCNS010000104         7564 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFCNS010000104 DAFCNS010000000
VERSION     DAFCNS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.107"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            533..1768
                     /locus_tag="LNE23_002552"
     CDS             533..1768
                     /locus_tag="LNE23_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932363.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            1852..2940
                     /locus_tag="LNE23_002553"
     CDS             1852..2940
                     /locus_tag="LNE23_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932364.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            2937..4358
                     /locus_tag="LNE23_002554"
     CDS             2937..4358
                     /locus_tag="LNE23_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBK6932365.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            4394..5110
                     /locus_tag="LNE23_002555"
     CDS             4394..5110
                     /locus_tag="LNE23_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBK6932366.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            5129..6712
                     /locus_tag="LNE23_002556"
     CDS             5129..6712
                     /locus_tag="LNE23_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HBK6932367.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            6847..7425
                     /locus_tag="LNE23_002557"
     CDS             6847..7425
                     /locus_tag="LNE23_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932368.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
BASE COUNT         2751 a          824 c         1359 g         2630 t
ORIGIN      
        1 aatgtggatt atcaccttcc aacagtatat aaaatgagcg taaaaacggc tatttttgcc
       61 ttaacaacga tttttttgta tacaaatacg atatgctcat taattattgg aatttgtagt
      121 gctgtttaaa ttaatgaagg tgtaattata tgagtgatca ttttgaaaaa gtttgttgag
      181 aagatgtgct tctagtcatg tgatgggaga aaataattta actgttagag tgaatagtta
      241 aattattctg gttatatgaa aaaagtagtt tctaaaataa tcttgcaggg attaacttaa
      301 agaaattgag agaatagttt ctttgaaatt tctatataca atgagaagct gaaatttact
      361 tttatttgat gactggaaat agaaattaaa tatattgtat tgttacatta aagaaaactt
      421 gtatggaaaa atcagtacaa taccattatt taatgttctt cacttaaagc gcaaaaaata
      481 tcagtttatt aaactcgtat atagaacaaa tatatgtaga cggggcgaaa cgatgagtgt
      541 aaaaaaaatg ctatttatgg taagtacaat aattgtatta acatcccgat tttggggaat
      601 taatactagt ttatctatgc gatattttat tatggcaata tgggatttat attttatgtt
      661 aagctatttt tcttatcgtg agaaaacata taaaaaatac gttatcaaga ataacttttg
      721 gttgactata atgccgtttg tgatattttc tatctatact ctaataatat ggggaattgg
      781 ttcaaatgca gaatttgaaa attttataaa gttatgttcc acgttgcttt atttaattct
      841 tgcatatggt tacgcatgta cagcatttta cttatttaag gcagaaggaa ttgattttat
      901 tttttggtca ggtgttattt catatatgag tggttcagtt ttttatttaa ttgtatctta
      961 tggtttaaat ggtttaattt cttatataaa aagtcttata acaggtgaaa caaatactgc
     1021 aaattttgct atggaggtac atgatttaac ttttgcgatg ggggtttttt tcctttatta
     1081 cgtatttttt gaaaataaaa atacaaaaaa acataagata aaaattatcg tatctttaat
     1141 tttaattttc atgggattga agagaattga actgttagct ttaagtggtg caattctagt
     1201 gtattatgtt ttattaaagt ggggaaaaaa gattcaaatc cgtgcattag tgtgtactat
     1261 ttgtgcaact gttatttcgt tagtgtttat ttacattata gatacaggaa ttttggcatt
     1321 gcttatgtca aatttgggca ttacagatga cggtgcaagg ctttcatttt atctatattc
     1381 atctaaattt tatgaacttg gtttaggata tgtaggaaca ggatggacat ggttttcaaa
     1441 atactttttt aatctttatt tatcgcactt taggttaaat ggtggaagaa ttgcagcaag
     1501 cctacacagt gatatattaa ctatttatat agaagttgga tgtatagttt ttattatatg
     1561 gatgttttgg ttattttttg tgaaaccgat aagactaact aaaaaatttg gattgaaaac
     1621 aggggagtgt gtattattat taactatata tatgtttata ttatatttga cggataatac
     1681 gctcacttat ccagacactc aaatgatttt tctcttagta cctatgatag tttcgtgggt
     1741 tgataatcaa caacgaatat cggaatagat agctaaagta tattttaatt atgaatttat
     1801 aattagttgt aaaagttaat atttaacgga caggaagtag ggtgaaaaaa tttgaaaaaa
     1861 attttagtgt tagcaaatac atattatcaa ataatagtag caatacaaat gaaaaataca
     1921 attttttccg atgagtatgt tgaaattatt atttctgatc attctaaaaa cactaaagaa
     1981 attgcgaaaa aaatagaaga aaaaaaagtg tttgatggct gtcattattt agaaagcaaa
     2041 tcttttgatg ataatattgg gtattttcaa aagattaagg actgtcttca gatttcttgt
     2101 ggtaaatcaa atcgatatgc ctattatttg aaaaactgta aaagtgaata ttttgatgaa
     2161 tttatttgtt tttcaatggg agaggtttgg ttggaaggaa tatatgcttg cttatatcga
     2221 aataataaaa atgtacaggt atcaatttta gaagaaggta ttttaagcta tggagttgac
     2281 attattccaa cagcaagaac aaaagctatc gcagtactca attacttaaa aaaaaggact
     2341 ccactatata acaaaagcta taatttttat tgtttttttc ctaatatata tacaggaaga
     2401 gagaatacaa tacaaattcc attagttaca gctgggtcaa gaaccgcatt gcaactttcg
     2461 gatattttta accctatgat agatatttat aaagaaaaaa aatatatatt ttttacgtct
     2521 gtttatgatt ttgagggcgg agaacctgtc ggagaatatg aattagtatg caaaatagca
     2581 gacttagtag gaaaagataa tctaattgtt aagacgcatc ctagagatgt aagaacaatt
     2641 tataaagata atggatttat cgaggataaa aattcatcaa taccttggga ggcgattcaa
     2701 ttgtcaggcg attttaacga taaaatattt atgacaatta attcaggaag tgttatttca
     2761 ggtagtacaa tgtctgcaaa acctgtacgc acgtattata tgtataaatt gtgtaacata
     2821 gaaggaaatc cgtcttgcaa aaagaatgcg tatgatattg agcgattgct taagcaaaaa
     2881 acgatgaaaa acattttaaa gaatgtgaaa atagcagaaa gcttagatga tatactatga
     2941 gcaaaactaa atatttaatt aaaaatatgg gaatattaac aataagtaat ttttcttcaa
     3001 aaattttagt ttttttgtta gtccctttat atacaagtgt attatccaca acagaatatg
     3061 gcacatatga tttgacaatt tcaacagcca cattattgtt tccaatttta accttaaaca
     3121 ttgtagatgc tgtgatgcgc tttttaatgg ataaagataa aaataaaaaa aatatagcat
     3181 ctatagcgat taaagttata tttttaagcg tgatagtctt tggatccgta atgtatttgt
     3241 taaatattac aaaattatgg caagatgttt atggattaga aaaatatatt tttttgtatt
     3301 atatatccta tgtatggaat caatttctaa tacaatttgc taaagggtta gaaaaagtta
     3361 aagctatggg catagcaggt attatcagca caattgtaac aataattgcc aatattttct
     3421 tcttgcttat tctgaaatgg ggattaattg gttttttcat tgcaaatatt ttatcacaga
     3481 ttatttcagc tacttatctg tcagtattag tgagaatatg ggaatatatc gattttgagg
     3541 ctaaagatcc tcagctaaag agagaaatgc taaattactc gattccatta attgcaactg
     3601 ttgttggatg gtggattaat agtacagctg ataggtatgt ggtttccttt atagtaggtg
     3661 tctcggcgaa tggcttattg tctgtggcat ataaaatacc tcaaataatt aatactttgc
     3721 agggaatatt tatacaggcc tggcaaattt ctgcaattaa agaatatgga gataatgaga
     3781 ctgcaaaatt ttatggaaat acatttgtta tgattaattt gttaatgtgc ataacctgtt
     3841 caagtcttat catattaact agaccgtttt cacatattct ttatgcgaaa gacttttatg
     3901 aagcgtggaa atatgttcca tttttattaa tagctaatgt tttaaattgt gcatcaggtc
     3961 tgttaggacc tatcttatca gcaaaaaaag attctaaatc catgatgtgg tctgcaatca
     4021 taggggcatt agttaatgtg attatgagtg tagtccttgt ttataagata ggggttcaag
     4081 gtgccacaat agccactgtt atatgttcat atgtaatata tgttgttcgt aaacgagctg
     4141 tgggaaaaga tatttttata aagcattatc atattgttgt ggaatcttgg ggattattgt
     4201 ttgttcaggc aatcatagaa atatatttta aaaattatgt aatagaaatt ataattatga
     4261 ttattttaat tattataaat atcagttata ttaagcagtt gtgttataga ggaagaaaca
     4321 ttatttcaat gattaagagt cgagagcaca aacggtagac gatgttattt ttataaaaag
     4381 atataattta ttaatggtcg ataattatga aaggaaaaat attcttaaaa aaaagagtat
     4441 aggatgtcgt atgaaaatag tcgctatagt accaatgaag ttaaataaca aaagattgcc
     4501 acaaaaaaac acgaaagctt ttaccaatgg aaaaccattg tgttattata tactttcaac
     4561 acttttaaaa gtaaaacaga ttgataaagt gtatgtctat tgtagtaacc cagatattaa
     4621 agaatttata ccagaaggtg taacattttt aaagagatct agttccttag atcaggatac
     4681 aacgagtatt acacaggtat tagccggttt cacaaaagaa gtagaggctg atgtatatgt
     4741 aatgacacac acaacagcac catttattag cgaaaaatct attgaaaaag gactagatgc
     4801 cgtgctgaca ggagaatatg attctgcatt tgctgcaaaa aaattgcaag atttcttatg
     4861 gaaagacggt gaaccattta attatgaatt ggacaatatt cctagaacac aggatttgcc
     4921 gcctttatac gaagaaacta gtggatttta tatttataat cgcaatgtga tgaccgagtt
     4981 gcatagaaga attgggaaaa aaccttgcat agttgaagta ggtgagattg agtcagttga
     5041 tattgatgaa gccgaggatt ttatgattgc tgatgcgatt tataatcatc ttattttaca
     5101 aggtagttaa gacaggggga aggttaggat gaacaatgta cagattttag attgcacact
     5161 tagagatggg ggctattgta acaattggga atttggattt gataatacaa aaaaaattat
     5221 ttctggactt atagaatcag gaattgagat tatagaatgt gggtttctca caaataaaac
     5281 agtatacaat aaaaatgtta caaaatatac gaccttagaa gaattagcac agataattcc
     5341 tgaaaaaaga gaaggaaaat tatttgtagc aatgatgaac tatggcgaat ataatataga
     5401 ggatcttcca gattatgatg gttcatctat tgatggaatt aggattgcgt ttcataaaaa
     5461 aaattgcaat gaagcgttga aattatgtcg tgagataaag aaaaaaggct acttagtttt
     5521 tgttcaagct atggtatctc tcaattatac agatgaagag tttttagcag taataaaaaa
     5581 tgtgaatcag tttaaaccat acgcttttta tattgttgat agttttggaa tgatgaaagg
     5641 aaaagattta actcgtcttt tttacatgat tgagcataat ctagaaaaag atatacagat
     5701 aggttttcac tcacataata acatgcagct tgcatattct aatgcacaaa aacttataac
     5761 cgtacaaaca aatcgaagcc tgattattga ttcttcggtg tacggaatgg gaagaggagc
     5821 aggtaatctt aacacagaac tatttgtaga atatttgaat gataatgcag gaaaaaaata
     5881 tcaattaaaa ccattattat atataattga tgaaatacta aatagtttct atcagaagag
     5941 ttattgggga tattcattac caaactatat ttcagcagtt cataatgctc atcctaatta
     6001 tgcaagctat ctgtcatata agaatacttt aacaattgaa gcaatgaatg aaatattcga
     6061 aatgatggat gaagaaaaaa aggtatctta tgataaacaa tatatagaag atttatacta
     6121 caaatatatg gaaactggga aaatacagga ggaatatatg gaagaactga gaaaatttct
     6181 ctcagacaaa aaaattctac tgattgcacc aggaaaaagt tctgtagatg aggtagaaag
     6241 aataaaagag tttgttacca aagaagaagt cgtaataatt agtgtaaatt ttgaatattc
     6301 tcatatagat atagattacg tatttttaag taatttaaga cgttttcatg aattaccgaa
     6361 agaaagcaga actaaatgta ttgttacatc aaatatacaa gcatcggatg tatatttacg
     6421 gacgagttat aaaaagttgt tatctgaaag ggaagttgta agagacaatg caggattaat
     6481 ggcaattaga tttttagccg atatgaatgt ggatgaaata ttcttagctg gatttgatgg
     6541 atattcacat aatgaagatg agaattatgg tgagcaatcc atggaaatta taacaaaatt
     6601 tgcattatta gatgcaatga acgtgcaaat gagagatgaa ttgtcagaat tagctaaaaa
     6661 agtaaaggtg acatttttaa cggatccacg ttgggttcga attgataagt agtagtaata
     6721 atgtttttaa aattttgata aagagatagt gagagaataa aaatgggtaa agtcttaaat
     6781 aaaaaagaag aaaaatattt aagggccgat ggggcgagat gaaactctaa ttttgaactt
     6841 ttaagaatca tttctatatt gtgtattgtt gcttaccatg ctaatttgcg tttaaattat
     6901 aatgcagacg gaattatagt acatggttca ttaactgcta accagatttt cttgaattat
     6961 attggtattt ggggtcagct aggagttaat tgttttatat ttatttcggc atatttttta
     7021 agtgagaaaa gaaacattca ctttgaaaat gttttgggaa ttgtattaga atgtattttt
     7081 tatagtgtat tagtttatgg aattctttgt gcatgtggaa tagaaacatt ttcattgaac
     7141 agctttgtaa aagaaatatt atcatttgct ctaggtagat attggtttat tacatcctat
     7201 ttagcattgt atattctaag tccgtattta aatatcatgc ttaacaatct ggaaaaactt
     7261 cagttgcaaa aaataagtat gttattgttt ataggtgtat gctgttatcg tatgttattt
     7321 aaaacatctt tgtttgggga ttgtgctgtt ttaattgcac tatatgtcat atgttataca
     7381 ctgaaaaaaa cgataataat atttttgaga gacacagaat cataggaaca attgtatgta
     7441 taacagttat accacttctc atatccatag gaatactttt caaatttata ccaaatgcaa
     7501 tggttaacca gatggttcat attggttctc cattgatggt tatagcaagt atatttttat
     7561 ttta
//
LOCUS       DAFCNS010000105         6928 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFCNS010000105 DAFCNS010000000
VERSION     DAFCNS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.108"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..513
                     /locus_tag="LNE23_002558"
     CDS             <1..513
                     /locus_tag="LNE23_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932369.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            516..1265
                     /locus_tag="LNE23_002559"
     CDS             516..1265
                     /locus_tag="LNE23_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6932370.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1277..1546
                     /locus_tag="LNE23_002560"
     CDS             1277..1546
                     /locus_tag="LNE23_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932371.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1551..1715
                     /locus_tag="LNE23_002561"
     CDS             1551..1715
                     /locus_tag="LNE23_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932372.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1708..2010
                     /locus_tag="LNE23_002562"
     CDS             1708..2010
                     /locus_tag="LNE23_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932373.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            2007..2168
                     /locus_tag="LNE23_002563"
     CDS             2007..2168
                     /locus_tag="LNE23_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK6932374.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2165..2470
                     /locus_tag="LNE23_002564"
     CDS             2165..2470
                     /locus_tag="LNE23_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932375.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2470..2826
                     /locus_tag="LNE23_002565"
     CDS             2470..2826
                     /locus_tag="LNE23_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBK6932376.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2813..3031
                     /locus_tag="LNE23_002566"
     CDS             2813..3031
                     /locus_tag="LNE23_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932377.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            3028..3447
                     /locus_tag="LNE23_002567"
     CDS             3028..3447
                     /locus_tag="LNE23_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBK6932378.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3444..4001
                     /locus_tag="LNE23_002568"
     CDS             3444..4001
                     /locus_tag="LNE23_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBK6932379.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3998..4294
                     /locus_tag="LNE23_002569"
     CDS             3998..4294
                     /locus_tag="LNE23_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932380.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4371..4784
                     /locus_tag="LNE23_002570"
     CDS             4371..4784
                     /locus_tag="LNE23_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBK6932381.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            5093..5245
                     /locus_tag="LNE23_002571"
     CDS             5093..5245
                     /locus_tag="LNE23_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932382.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(5242..5517)
                     /locus_tag="LNE23_002572"
     CDS             complement(5242..5517)
                     /locus_tag="LNE23_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932383.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            5971..6177
                     /locus_tag="LNE23_002573"
     CDS             5971..6177
                     /locus_tag="LNE23_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932384.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            6373..6540
                     /locus_tag="LNE23_002574"
     CDS             6373..6540
                     /locus_tag="LNE23_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBK6932385.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            6566..6910
                     /locus_tag="LNE23_002575"
     CDS             6566..6910
                     /locus_tag="LNE23_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK6932386.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         2512 a         1081 c         1522 g         1813 t
ORIGIN      
        1 gtacagcaac gaaacttcat ctatgcgctg ataggcgata tttttatcga tacaggcatg
       61 ccaacggact tctggaagga attcttctac tttcgttttg aaggtgtcac agggcgcaaa
      121 ataagcctga aagacgaatc gaatacaact gtgagtgatg ccaatgtctt agcaaatatc
      181 atcttagatt tcatctttga acatcatatt cctttcaaag aaggctatga gattttacct
      241 gcgaatcaag aatattactt ctacaaatgc atcacaaaaa gagtctgctg catctgtggc
      301 aaaacaggag ctgacatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa
      361 gttgatcatg cagagtacac atttgcagca ctctgcagaa tccatcacac agagaagcac
      421 aaaataggtg tgattaattt caaaaataaa tatcaaatca aagggatcaa attaaaccag
      481 aaaacaatca aaaagttaaa tattggaggg taaaaatgac agaacatcga agttattatg
      541 cgattatacc agccaacgta aggtacgaca aaagacttaa accaaatact aagttgttat
      601 acggagagat aacggccttg tgtaatgaaa gaggcttttg ttgggcaggc aatgagtact
      661 ttgcagattt atatggtgtg aataaagaga ccatatcgcg atgggtaagt gatttgatta
      721 agtttggata cttgaatcgg gaaatcattt acaaagaggg taccaatcaa ataatcaata
      781 ggtacctacg aattaatcaa taccctattg acgaaaaacg caatacccct attgacgaaa
      841 aagtcaaaga taataataca tctattaata atacatttaa taatacaaaa gaatatataa
      901 gagagttacc gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa tacggagagt
      961 ataaaaatgt tcttttgtca gatgagcaga tggagaaact caaaacagaa ttccctaatg
     1021 attaccaaga gcgaatcgaa cgactgtcag agtattgtga atcatctggt aagacttata
     1081 aaaactattt ggcaactatt cgaagttggg caaggaaaga aaaaagtgaa cctaagaacg
     1141 caagcagtgg atacaagcgc acaggaagac gagagaagct tcctgaatgg gcaatcgacc
     1201 aagaagccta tctcaagaaa aaagcgctag aacgagctaa tagacaatca aaagcaccat
     1261 tctaagaggt ggaaaaatga agatcgatta tctagaacta attaatgaaa tagcgaatta
     1321 taaaaagggc gaggaattag acgtcctgag agacgtatat gatcaactcg aagaagctgg
     1381 aattgaagga attaagaatg atcgttcgag ttggagtaag ctcagatact atttcgcact
     1441 ttatatcgat acaacacaat taagaaattt agcatatacc aaattactat ttgttgattg
     1501 tgttaaaggc ttgcaaaaac atcttaatga acttgagcag gtgtaatcag atggacctaa
     1561 agacatttac agcacagatt gaactaatgc atcaagaagc tttaagacaa agtgtatcgt
     1621 acgaagacaa gtggctcaac acgttccatg gcggacgtga gagcgcactt gatcaagtac
     1681 tcaaattact gaaaggagaa tgtcgggatg gataagaaag cggcaatgca gcgaattatc
     1741 gaattgactt attcagaaga ttggcaaaat gacaaagaag ctgcttcaga agtgatgagg
     1801 cttggaagag cgatgtgggc agacaagagc aacaagccaa gaccacgaaa aatcgcaatt
     1861 tggcacggtg acaaacttct agtgataggg acagctgaac agttagcaag tctcacaggc
     1921 ttgcacgaga aaatcgtgag gaaaagagca aggtgtggct acacagacgc taagaagaga
     1981 acgtttagat acgtggagga atcatcatga caacagaaga agtgattcaa atgcgtattc
     2041 gaagccttca gcgtgagatt gacgatctgg aacgaacaaa ggcagtgatg gtcaatgaaa
     2101 cggcgagaaa ggcaatcgat ttgcacatag agaatttaag aagggaaatc catcgattgg
     2161 aggaatgagc gtggataaga aagcagcaat gaaaagaatt gctgaattaa ccaagtcaga
     2221 atcttggcaa gaagacaaag aaatagttgc agaagtccaa aagctcggta aatcaatgtg
     2281 gactgaaaag cccaaacgga gaacgccgag aaaaattgca atctggcatg atgaccgaat
     2341 tttagtaaca ggtactgctg aacagttatc tgaaattact ggattaagca aaaacattat
     2401 ctgggataga gctaggagct tatggattga ttcaaaagga cgacagttta ggtatgtgga
     2461 ggagagataa tggatctcat tacacaatac agtgacatca tcctcaagaa aatcatgatg
     2521 aagattcaga aagataaaaa atcaaaagaa cgagctgaat tagttaagtt agaaatggct
     2581 gaaacaggag caggagtgcg aagtagcagg cattggaaag cagcagcaaa cattgaattt
     2641 tattacaacg aaattcaaaa agggttcgat cagatgcgtg agctggatcg gcaaacaaat
     2701 tggagcaaga aacttcatca agatcgtttc aaatttgtag agaagtataa agaaatatta
     2761 gaagagtatt tgaggaggac agcaaatgat aaaaaaactc gttcaattca gcatggattt
     2821 atatgatatc gaatcaggag caacactatc tgtggaatcg gaccatctaa tcataaattt
     2881 tggtggaaag cgccagatta ttttgtgggt agttgatgat gtactgtttc cagaaattgt
     2941 tcatgatttc gaagaatcaa aagcggttga gtttgaaata gtgaaaaaag taatggaatt
     3001 gattgaaaaa tacgaggagg acagcgaatg ataccgaagt ttagagcgtg ggataaacga
     3061 aagaacgtaa tgagagatgt agccgtcttg cattttacta aaaacggcaa aacaaacttt
     3121 attgaatatt ggataaatcc taccgaattg aaatcatatc acgtgcgaaa catcgacctc
     3181 atgcaatcca caggaatgaa agataagaat ggtgtggata tatttgaagg ggatatagta
     3241 ttagtcagcg tgcgaaatgg cttcgattac ttagataata aagtctgtat tgtcaaaaat
     3301 tcaatagatt attccggatt agtttgtgcc actgttgatg aagacttaga gtatcaaatt
     3361 tttaacacag agctgtttga agaatacacg tacgaagtca ccggaaatat atacgagaat
     3421 agcgagttat tggaggaaca gcgatgaata aacaggaagc aatcaaaaag ttagaaagta
     3481 ttaaagcgat aggaaatgat gcaatagctg cttgctataa cgagagtata aattcaggta
     3541 ttacgttaat gaaaaaaata gacgaaccgc agaaaccagt tattccacaa ctcgtggccg
     3601 gttggcttga gaaatctacg gaccctttta caaaagctga aaaaatagcg tatttaatca
     3661 aatctaaaga tggtgattca tattatttct gtgattggtt tgtacgagat ggcatagtga
     3721 cgcaagagca aggagaagaa ttactcgctt gggcaacaag acaatcatat gaaacactat
     3781 tgagcctata caacggctac gaggttgaga aagagccgtt atgggcaata aagaatgccg
     3841 atggaaacta tcttactaaa tgtgctttat ggggaaaaga tggagtaaat tatagttttg
     3901 aatgcaatcc atctcatcga ttgcttttca ctgataaagc aacagcggat gctgcagcat
     3961 tgttggtgaa tggaacagtg gaagaggtgg tagaaagatg aaactaaaag acggatttta
     4021 cgctagcagt cacggtatcg gcggtttaat gctagatatg ccgacaaaga accctaaaac
     4081 acgcaagaaa tcaaaattca aagtcggtga catggtccgc tgtgaagcag aagagttcgt
     4141 ttatccgttc agaggatacg ttaaaaagat actgtcaaac tcagcaatca ttcgtattga
     4201 aaatacaatg aagtgtgatc gttcgactgc aaaacacaag cattatttag ctgtagcgag
     4261 attagttgac atggaagtaa tcaagagcaa ataaaaaaag ccggatcgct ccgactgatt
     4321 caataaatcc aacacattta ttatatcaca taaaggagcg gtttgacttg atgcaattgt
     4381 tacgagaggt agatttcaaa cagacaagat gtaatgcgag agatgtgctg aagaactttc
     4441 ggcgtttgga gcggatggca ggtcgctctt tgatagatat taagtcgccg attataacgg
     4501 atatgccgaa ggcaccgaag catggcaata aggcagaaga cgcgattatt cagatgatgg
     4561 atatagaaac ggagagagat gcgattctag cggctttgat ggctcttagt ctgattagtc
     4621 gtcagatact ctactacagc ttctgtgtgc cagatagctt ctcaaactac agaattagcc
     4681 gtgaagtggg ttattcagaa agaagtatac aacggatgaa gtcggaagct ctaatagagt
     4741 ttgcagaagc atataaacac ggaaaaataa ttgcttataa ataatttggc ggttttttgg
     4801 cggaatgatg gcggttttta gacatttacc agtgttatta tggtagtgtc gaaagattag
     4861 gaaacaggat cgacaaaata aacgaaggga ggcaatctcc ctcatcgttt taaacttctt
     4921 cttagataga cagcaactaa ttatgaagaa aaggatgtga attcaactcc tcctaaattc
     4981 ttcatcggtt gctgtttatt ttgattagac agcagcactc acaaaactaa acatgttctt
     5041 gtcgtctttc aactgctgct gtctattaat ttatgtattg gagggaagaa aaatgaaatt
     5101 atcagtagaa ggcactccag aagaaataca agaattgctc caagctattg gtagtagcaa
     5161 ggagcaaaag gtaagtgttt ctaattcaga tattgacggt atttgcaatt gtttaaacag
     5221 agaaaaaata tctaatcctt actaagagaa aatatgttcg ttaatgaatt tccaattttc
     5281 ctctgtagct ctaccttgat aatttttttg cagttcacag atgaataagg aatcgttatt
     5341 atcaagtact gtatttaatt tttcacacat ttgatttgga ttcaaattag ttttaactaa
     5401 ccaaaaagat ttttgtagct ttatatatgc cccaaattct tttattattt caaatacttt
     5461 atcatacttt tgccctgggt tattaagatc ataactaatc atgtagggtt tatacatgtt
     5521 gtcttgcaca tccttgtttt atatttcagc ggaccactcg ctgataaata aaattatacg
     5581 cttagtattt attttcacaa tattaattta tcgtaataac ttttgagtaa atagaacaaa
     5641 aaacctgcac tagtttccgc tagtacaggc agtgactata tcggttgata atctagcata
     5701 tattaaaaat aattgcaaga aaagaaattt atagtggttt ataccaaatt atcaaagcaa
     5761 tgttattttg ttgctgtcta ttgtttttta attattcaca cgataactaa aggtgggtga
     5821 agagaaatga ttccattaat aatttcaatt tttgcgctct gtcttaatgt ctatatgatt
     5881 ggatttaaaa atgggcaaaa taaaaaatag tagcagccaa gaataatttt atagtgtcac
     5941 tgtggcggaa agggtagacg ctaagcatgt gtgctaggtc aatgcttcgg caaccatgca
     6001 aggttcgatt ccttgccagc gacattaaat gcctatgacg gttacgacta ccgaaaaaag
     6061 atcgttaaga agctatacgg tgctacgtac ggcaatgtag taagtgtgct atctgtacac
     6121 caccaagctt cggtcactgt ggcggaagta gaagacgcag cggtaaatgg cgagtagcct
     6181 cgtgagagcc tggtaagttc tcgtgagtgg tgcaatccca ctccagcgac tttaagcaac
     6241 cgaggcatcg gcggtttaaa aatatagggg tgcgcaattt cgtacgcgtt ttgtgcatcg
     6301 tgcaagttac tattacatat tagatcactc tttgagtggt ctttttattt ttgcacaaag
     6361 gaggaaacaa caatgtatag accacaatac ttagaacaga agtatgaagt aatcactgtt
     6421 cataaaggta atggcgaaaa agtatatgag tatagaagac caataaagag cgatacatat
     6481 aaacgaaagg aaaacaatga agttattcca ttgtatggca aaagaatagc taagcattaa
     6541 ataagattgc gaaaggagac ggaacatgac cgaggaattc tatagatggc tattacagtt
     6601 gacaagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca ggcttagaga
     6661 gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag gtaagtatca
     6721 tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact tagctttaga
     6781 tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg gcagatatct
     6841 tacagcatta gataaacaag agaagaagat agaaagcttt gctaacttcg atgcaagtga
     6901 aaggtggtaa gtgcatgatc atcaatga
//
LOCUS       DAFCNS010000106         6900 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFCNS010000106 DAFCNS010000000
VERSION     DAFCNS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.109"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..442
                     /locus_tag="LNE23_002576"
     CDS             <1..442
                     /locus_tag="LNE23_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBK6932387.1"
                     /translation="EKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRKV
                     EAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKSM
                     TNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            442..1641
                     /locus_tag="LNE23_002577"
     CDS             442..1641
                     /locus_tag="LNE23_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK6932388.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1657..2301
                     /locus_tag="LNE23_002578"
     CDS             1657..2301
                     /locus_tag="LNE23_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932389.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2312..3118
                     /locus_tag="LNE23_002579"
     CDS             2312..3118
                     /locus_tag="LNE23_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932390.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3140..3370
                     /locus_tag="LNE23_002580"
     CDS             3140..3370
                     /locus_tag="LNE23_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932391.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            3884..4192
                     /locus_tag="LNE23_002581"
     CDS             3884..4192
                     /locus_tag="LNE23_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932392.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            4194..4616
                     /locus_tag="LNE23_002582"
     CDS             4194..4616
                     /locus_tag="LNE23_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932393.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            4636..6225
                     /locus_tag="LNE23_002583"
     CDS             4636..6225
                     /locus_tag="LNE23_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932394.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            6237..6572
                     /locus_tag="LNE23_002584"
     CDS             6237..6572
                     /locus_tag="LNE23_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932395.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            6713..>6900
                     /locus_tag="LNE23_002585"
     CDS             6713..>6900
                     /locus_tag="LNE23_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932396.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         2646 a         1036 c         1419 g         1799 t
ORIGIN      
        1 agaaaaacaa atggaagaac aagacttcaa acgcttcttg gcgatgattg atgagtgcca
       61 taatgcgatt aatccagatt ttattgaaac ggtaaattat gtcacaaact ttcaacgaga
      121 aatgcgtaaa gtagaagcaa tgactgtttt agctacacaa tccccacaag aaatggtgcc
      181 agaaaatatg tcttctgata acttctcgaa actgaagaaa gtctttgaat tttcttctac
      241 gaagatcttt atgcgtatgg atgaatctat tttacgtcat atagaaggat tagtgggtaa
      301 atcaatgacg aactcagaat tagaatccat tcctcaacaa aatcaaggag acgctgtgat
      361 caattttggc tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac gtcaattaaa
      421 attgtttgat ggaggaaaat aatggaaaac caaaatgaaa gtttaatcaa acagtatgtt
      481 aagcgtcgtg caaagcggcg cttatttttg tgggtgtttg gtacttctgc aggtttgatt
      541 actatattga tcactgtgtt tgtgaccctt tttctaatac ttgcggcagg ttctattgat
      601 aattctgatt caagtagtgg tggcgaagct tttacaggag aatattcgga aggcttaccc
      661 atttataaag aaatcaaagg aagaggccct ttttctgatg agattgcgca atatgctgta
      721 ggtgcagcag tgaagtataa attattacca agtgtgatcc tttcgcaata cggttatgaa
      781 tcagcgtttg gtacgtcggc gtccgcaaga aatgatctca attattttgg gattacgtgg
      841 tttgatgggt gtctgtttcc taaaggaact gcgcgtggaa ttggtggcat tgaaggtggc
      901 tggtacatga agtttcccaa ctcaaaagct gccttctcct attacggctt tatggtagcc
      961 acacaatcaa attttaatgc ttgtgtcgga aataaaagcc ctggcgcaag cttattgatc
     1021 ttaggacgag gagggtatgc cgccgcagga attactgaag acagtgccta ttataaaaat
     1081 tgtatgtcta ttattaatac aaataaattg atggagtacg atgagtttgc gattaaacac
     1141 tggagagaag gcggcggaac tggtggtacg attacaggaa gctggggcaa tccattccca
     1201 ggaagtagct tagataaaaa ttcgttcagt ggaggacaac tttttgggaa aaatccagga
     1261 ggagaattcc gacctaacgg gttccatgat ggactagact ttggttcagt cgatcatcca
     1321 ggaagtgaga ttcatgcggt tcatggcgga aaagtcgtgt acgttggcaa tcctggtatt
     1381 tcaggtttgg gtgcgtgtgt gatcgtgatt aattacgatg gcttgaatat ggtgtatcaa
     1441 gaatttgcaa atagtacagg aaattctcga gtgaaagtag gcgatcaagt gaaagttggt
     1501 caagtaattg ggatacgaga tacagcacac ttgcatttag gatttactcg aatggattgg
     1561 cgtcaagcac aaggacatgc attcattgat gatggtacat ggatcgatcc gttaccattt
     1621 ttgaatagca gcaaaaaata gatcaggaga aaaaatatgg aaaataaaac cttaaaaatc
     1681 gtcttaacag ctgcaattag cgttagttta atcggcaata ttgcgttgtt ttggcagctt
     1741 aaagaagaac aagagacaaa tacctataaa attgaagaag tggctaaaaa cgcgtctgat
     1801 aagctcaaaa aaaattcttc tgaggcacag gcgaagatcg acaagttaaa tcaaaccatc
     1861 aatgagaaaa accaagaaat cgatcagctg aagcaacaag atggcacaaa agcaaaagag
     1921 gaagtaactg aagcacaaag aaagacggca gaagagttcg gtcaattggc cattgataaa
     1981 tcattaagca gaaatgaatt aacggaaaaa ctaaaagatg tcgcaaccca agaagtgatc
     2041 gacaaattaa gtcctgcaga cgatgatcac caacatgacg actacggaag ttactctttt
     2101 acattaggtg acgtgcaaac ttacgctcaa acaagcagtg taaaagagga acaaaatttt
     2161 gtcgtatttg ttgattatac aatcggcaat cctcaattta aagatgtcaa accacaaaaa
     2221 ataaaaggtg gattaactgt cactgaaaaa caagtagatg gtcaatggaa agtcacagac
     2281 tttagttatt tcactcgtta ggaggaagaa aatgagtaag aaaaaaggag caagacaacg
     2341 ttttaaaaaa cgaaaaagtt atcttagatg gctggggatc tttatgattc tcggcttttt
     2401 gattcttgct agtccgattt acacacataa aaaggtagac ataccagatg aagtaagcac
     2461 gaacgcattt ttaaacacga aggaactgac gatggtttca aaagaaaaag gagcgaacca
     2521 gcaattagtt tttgaatttg cgatcgacga tgaagatcag agcttattaa aagaactagc
     2581 taatcttaat tatcgtgtag aagtaaagac agctaaaggg gattatcaag caataaaagc
     2641 caaagtgatc aaggtgtctg atgattattt tgtagtgaag ttagcacatg taccagaaga
     2701 atatatcgcc atgcgtttaa ccattattcc tgaaaaaatt gatccaaaag tggatatgca
     2761 agaaccacaa gatctgattt attacgttca cgaagataag gttaaagatc aagtgaaaga
     2821 tgaagattat gaacaacatg cgatcaatta caaagtgaaa ggatacaaaa aagaacaaaa
     2881 agcagcacaa aaacaaatgg aatccttcca agcaaccatt gatttgaatg aagaattagt
     2941 gaagaagcta aaagatcaat tgccttatca agtcgcagat gatcaggaaa atactgaaaa
     3001 caagattaat agctaccagc aagaaattga aacatcgaaa acgcaaatga aagatcaaga
     3061 agagatcatt caaaaacttc aagaaaaaat tgatcgtatg acaaaagaaa agatttagaa
     3121 ggacaggtgg agtgaaaaaa tgaagcgaac aaacttggct gacaaagata ctttcgctga
     3181 gcagaaagta ttgattaaag cctatctcaa agttgtaaaa gctaaagaaa tatgtcgaga
     3241 agtgaattta tctaatacaa caatatctga gttgaagaat ggtcgaaaga atttagatac
     3301 tttaaaattc agatcatttg aagctctata tgtagctgct aaaaaatatt ttacagctga
     3361 taacaaataa aaaacaggag tattttatgt gaaaaaagta tataaaaaat gcttatgaat
     3421 cagctatatc accttaacca tttgttaatg agatgggaga aaacattaac cacgattcta
     3481 acgcaaacat caagcacata aaacatttaa cactcctcct ttctggctga agatttcaac
     3541 caaacaggaa gacagcgtat caattatagc atacactttt tcttaaattc atttttcata
     3601 tgacaaaata tttccttcta atcgacaaag ttagaaggat tttttattgt ctaaatacac
     3661 aaataacctt aatttataaa gagcataagg aaacgtggga acacttttta aaaaaagtgt
     3721 tccaataggc tgtgcctatt acgtttctca aaaataattg tgggaacaaa atatttaaaa
     3781 ataggatagt gaaaggatag caaaggatag taaaaggata gaagcaggat agcgaaaagg
     3841 ataggctcct tttcgctatc ctttttaaat tggaggaatt aatatggaag taagaataag
     3901 aaatgtagat gagaaaatcg taagagaaat tgatcgaaga tgtgacgaac taagtaaccg
     3961 aacgggtaat aaatggagtc gtaacgatta tttaaagcta ctcgttgaaa atgattttga
     4021 tcgtcctttg atggaatata aacaagataa atttgatcaa ttattaaatc agttaataga
     4081 taaattttct tttgttcaaa gtcataatac aaaagtatta gaagaatata tttgtcagaa
     4141 taatcaaatt atcgaacttt taattgaaca aagtagagga ggagaagttt aacatggcca
     4201 aagttataga caagcatatt tatttttatg aaacacatat agatgttcta gatgaaatta
     4261 ttgcaactca tgcagaggtt aaaaactata gtgaagccgt tcggtttcta tgtatgaatt
     4321 atgattcaga agtggagaaa aaagtaaatt ccaatcaagc aaagttgaat gcaatgggaa
     4381 aagaaatttc tattttaacc gaaatggttt cagagtttgc ggatctccat ttgaaagata
     4441 cagggatttt aattggtaca aattcaactg tttaccaaga agcaaaagag cgtgtggaag
     4501 gcaagataaa agcaaatcaa acgaagaaat attctcctaa gataaagaaa acaataagcg
     4561 aatcagtgac tacaaaaagt gaagtatttg atccaacaag gttcttaaaa cgataaaaat
     4621 tagaaggaga atgtgatgag taaatcacct gcagtcattc tcacttgtca atttaccttg
     4681 cccaataaaa aatctttttc aacatacatt gattatatga caagagaaaa agcgctggaa
     4741 gaaaaggaac gacgtacgcc cgaggaagaa caggaactgc gtctaatcaa gaatgcttta
     4801 gaggattttt atattcctga aggagaaact ttttcggaga gtaagacggg taaagaattc
     4861 agcgaaacgt cgaatgaagc gcaatcgttg atgaaacatg gaatcaattt tgacaagtta
     4921 gaagaacaag attttaccaa atatctttct tatatgacac gtcactatgc gttagaacaa
     4981 aagaaagtac tgacgcatag tgaacaaaaa gaataccaac gcttgaacca agcaataaaa
     5041 aaatatcaaa cagagccaca caaaaaagct tcaggaaatc ttcctggagt tttttctatg
     5101 gcttcagacg aagtgaaagg aagtgatctg aaggagatca agcaaacctt ccaaagagga
     5161 caacaaaaag gctcaatcat ttatcaagat gttgtctctc atgatcattc gtatcttgaa
     5221 aaaataggat tgtacgatcc caaaacagat actttagatg aagaaggttt aaaagcagct
     5281 ggaagaaaaa tgatggagac attgttcgag aaagagcaac tgaatgaaac aggctactgg
     5341 atggcaacga ttcaccgaaa taccaaacat atccatattc attttgcagt tgttgaaaaa
     5401 aaaaatacgc gtaaaccata cactgagatt gaaaatggtg tgagttatat cgtaccgaaa
     5461 gggaagagaa ggcaagcaac attagacgaa atgaagacta attatgtaca tgagttagaa
     5521 cgtttttctt atgaaaaaaa tcgaatgggt atggagaaac gaaatttatt gacacggaaa
     5581 agtgatttgc gtaacacctt aaccaaagta gtgaaagagc cagtacgtta tgatcgacac
     5641 gcaatgagct tattaactga agtatataaa aacttaccta aaaagcgttc ggaatggcat
     5701 tacggggatg aaaatcgaac caaactttca ccatacacaa gagagaaact cgatgagtta
     5761 acccaatatg tgttaacaaa agaacctgga tatcaagaat atatacagat ttcaaaagca
     5821 ttgaaagaag aagcaaaaag tctttatggg gaaagcaaga gagaatcaaa ggatgcagaa
     5881 aaaaatgctc tctttgattt aaaaaagcgc acaggaaatg ccattctaac agaactgaag
     5941 aaacaggata gtcaaataaa acctttaata gaaaggatgg atcagatgca atctatttac
     6001 aatgaacgat cctttcgaac acttaaacag ttgccaacga atgaatacaa atatagttac
     6061 gaagaattca tgaagcgcag gaaacaattc caacaaagag ttgtcacgca acggagtttg
     6121 cgtcaattaa atcgaacgat taatgcccat gaagaaaaat accacgcaag taaagcatac
     6181 gaagagcaac aacgaagaat tcaaatggaa caagaaagac agtaggagga atagcaatga
     6241 aacaatattt agatcaatgg aaagttatag aaggatcatt aagagaagaa cgaatcgaac
     6301 aacttcccga ttgtttagaa aaagagcatt tgtttcagat aagagaaatg ttgataaacg
     6361 aacagtttga tccaaatcaa tttttagtcg ttgagtaccc agctactggt gtgtactgtt
     6421 gtaatcatgt gaagggtgaa aaatatttta tcattcaaga atatgagggc aagttagcgc
     6481 cttattatac tacttgggaa atgaatgaag aggggatcaa taacttcccg tgtaaaagca
     6541 ttgaagagtc tatttcactc acagaatgtt agttgttaag tgaataaaag aagtaaaaga
     6601 gtcctgaacg tgattgtcca ggactctttt ttttcactga attgttttcc gccaattcat
     6661 tgagtggtgc gatttccgtc tacattatat caaaagaaag gaggaagaag aaatggctta
     6721 ttcaacgatt ggagttgttg ttgaaaaaag cagagacaat cttgtttttg tgacagaaat
     6781 tcaaacaggc agagcttttg tagtcacaga caaagcggca aaagcatatc aaagtggcga
     6841 tattttaacg ttaaatctga ctacgaagac gttcgtcgat gcggcagaag attatccatt
//
LOCUS       DAFCNS010000107         6869 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFCNS010000107 DAFCNS010000000
VERSION     DAFCNS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.110"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..227
                     /locus_tag="LNE23_002586"
     CDS             <1..227
                     /locus_tag="LNE23_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBK6932397.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..574
                     /locus_tag="LNE23_002587"
     CDS             227..574
                     /locus_tag="LNE23_002587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932398.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            571..1620
                     /locus_tag="LNE23_002588"
     CDS             571..1620
                     /locus_tag="LNE23_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6932399.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYDHLNKTEFNIYKLIESVIENFAS
                     KIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVL
                     LIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKS
                     KLGLGTKFVIKLPFQ"
     gene            1635..1841
                     /locus_tag="LNE23_002589"
     CDS             1635..1841
                     /locus_tag="LNE23_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932400.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="LNE23_002590"
     CDS             1862..2458
                     /locus_tag="LNE23_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HBK6932401.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3410..4009
                     /locus_tag="LNE23_002591"
     CDS             3410..4009
                     /locus_tag="LNE23_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6932402.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4058..4774
                     /locus_tag="LNE23_002592"
     CDS             4058..4774
                     /locus_tag="LNE23_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBK6932403.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            4788..5567
                     /locus_tag="LNE23_002593"
     CDS             4788..5567
                     /locus_tag="LNE23_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBK6932404.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            5650..6006
                     /locus_tag="LNE23_002594"
     CDS             5650..6006
                     /locus_tag="LNE23_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6932405.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            5996..6262
                     /locus_tag="LNE23_002595"
     CDS             5996..6262
                     /locus_tag="LNE23_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6932406.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            6421..6699
                     /locus_tag="LNE23_002596"
     CDS             6421..6699
                     /locus_tag="LNE23_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932407.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(6696..>6869)
                     /locus_tag="LNE23_002597"
     CDS             complement(6696..>6869)
                     /locus_tag="LNE23_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBK6932408.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT         2527 a          957 c         1333 g         2052 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatcatt
     3001 tagttcttat aaattagaca agagaaaata aatattccat atctacttta ttggatccat
     3061 aacatgacta aattctgaat actttttaaa gcttatcatt tggtaaaact gaatataagt
     3121 taattatata gactgaaaat tttgaatttt cagaatagaa taacttaaaa gtactgacaa
     3181 attttttaaa ggggcaatat taatgttctg agataatcaa atcttaccga aaatatcaaa
     3241 agtccaatgt gacacaattg gattttgtca cattcgttaa aatcgctcca tccttagagg
     3301 ctctaatagg aaatcacttt tcttttagcg ccttttttct atagcttgtt taaatgtgac
     3361 ttattatctg ttataatcgt attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt
     3421 ggaaccgatt cgcgacaaaa aaaagatcga tgccatgaag gccattttgg cttccggaaa
     3481 atatgggcaa cgaaatttgg tgcttttttc gatcgggatc aatacagcct atcgaatctc
     3541 cgatttaaga cagttaaaat taagggatgt gctagaaatt tcccgtgggc gggtgatcgt
     3601 caaagaacgt ctggccatga aggaacaaaa aacggccaaa cacaattcgg tcttcatttc
     3661 gaacaaattg cgaaaagtga ttcttgatta tgttcaatcc gaatttcctg agcagttgca
     3721 agcacaagac ttcagcaagt atttgtttcc aagtcgaaaa ggagcagata cgcctttgac
     3781 ccgacaaagc ctttggcgga tcattcatga agcaggaact gcagttgggt taaaagaaat
     3841 tggtccccat tcgatgcgta agacctttgg ctatttttta tacaagcaag gaaccaaaac
     3901 agagatcatt cagtcgctgc taaaccattc ttcacaacgg gagaccttac gctatattgg
     3961 aatcacccag gaagacaaag atactgcagt aaagagtttg gatttatgac agaataatgc
     4021 cctatataat agaaggaaaa attcaagagg tgagaaaatg aacgaagaaa agcagcctaa
     4081 ctttcctgat aaatatcact tatctcgcaa agagtctgtg tatttattga agaaaaacat
     4141 tgtagaactg gtgtataatg cggggaaatt tgaagggtta aatacaacgt tgttacaaac
     4201 agaagggatt attaagtata atcgggccaa caacgtggct gtagatgatg tcctgaccgt
     4261 cgttaactta aaaagaggct ttgaaatgct tttaaatgac gtacaggagc ccctgttaga
     4321 gacaagtaag cggattaatc ggatcgttgc agcagaggat gcgctttttc ccggtgaaat
     4381 acgaaccggt ggggtagaat tatcgacgat tcaaggaaga tatgtgccac caatgttaac
     4441 tgaagacgag gtaaacaatc aatatggtga gataatgaat caagagatat cagatacgga
     4501 aaaagccctg cgactgtttc tattcatttc gaaaaatcaa atattctgga atggtaatgt
     4561 gggtacagcc ttgttgattg cgaataagat tatgtttagc aacggtttag gactattatc
     4621 aatacccgaa aatgtattta tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa
     4681 tctattagtc aatgagtcaa atattatatc atttttatat gaacagtgta tctttggtat
     4741 taattatttg aaaataatcc aacatacaat ttaagaggta taagtaaatg atgactatag
     4801 acgaagcaaa acaattagca acttattttt cagttggttc agcgggatat attttagact
     4861 ttactaatgt aacgtggaac atatttacaa agaaaatcgt agggattgaa atacaaaatc
     4921 attatggggg ctcaaaggga gtatcctttc aaaagtttct gcttgacgat caatttgatg
     4981 attcgttaaa gaatcggtta atccaatctt taaaaggtat tatggatcga gataaaaaat
     5041 atgaatctac tgaaatgaat cccatagttc agttgctttt tgaggaagta tttaaagaag
     5101 atacttcaac tgcattaaga aaaatgaaat tttcaagcga gattgattta ctttatattc
     5161 ggcagctacc cattaggatt caaacagata ttaaaaacaa ggattttgac tctgttttga
     5221 caaaagcaaa tactttaata gatgaagttc taaaatttat tattgaaaat accgaaggac
     5281 cgagtgttaa tattaattta tttaagtcaa aagaattaag aaaagaagta tttggccgcc
     5341 tcaatattaa aactgaaaaa gatatggata atagaatcaa agcccttgtt ggagcactca
     5401 atacattatc agataaaata ctcgaaatga gaaatagtca aggagatgct catgcacatg
     5461 gctcaaatag aatagtaatt aaggaagaag aagcaattct tgttgcaaat tcttctatgg
     5521 ttttgtgcga gtatgtgttt actaagtata aaggattaaa tagataataa aatgcatatt
     5581 ttatagtata caattcgaat ttttatgtta tatactataa tataaaatca atatatgata
     5641 taattgggta tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa
     5701 tttttagaat ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata
     5761 gaagaaacag aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta
     5821 gacactaatt tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat
     5881 ttccatgtgg acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa
     5941 acaccaataa ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat
     6001 gattaaaaaa ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg
     6061 tgaaggaaga aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct
     6121 aactcaagcg gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga
     6181 gagaggggat aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa
     6241 gctggtagta gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact
     6301 aatcaattag tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa
     6361 aagacaataa attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa
     6421 atgaaaacca taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa
     6481 aggaatgaag tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa
     6541 gtgattttga tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc
     6601 tctgggcaaa gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt
     6661 aaaaataaaa tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc
     6721 ctgatttcag tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag
     6781 attcctcgaa tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac
     6841 aatttatgtc gacgttttat cgatcgatg
//
LOCUS       DAFCNS010000108         6569 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFCNS010000108 DAFCNS010000000
VERSION     DAFCNS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.111"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..579
                     /locus_tag="LNE23_002598"
     CDS             127..579
                     /locus_tag="LNE23_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932409.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="LNE23_002599"
     CDS             593..1282
                     /locus_tag="LNE23_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932410.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="LNE23_002600"
     CDS             1407..3758
                     /locus_tag="LNE23_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBK6932411.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="LNE23_002601"
     CDS             3838..4233
                     /locus_tag="LNE23_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932412.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="LNE23_002602"
     CDS             4558..5175
                     /locus_tag="LNE23_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6932413.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="LNE23_002603"
     CDS             5216..5515
                     /locus_tag="LNE23_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBK6932414.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="LNE23_002604"
     CDS             5549..5716
                     /locus_tag="LNE23_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6932415.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="LNE23_002605"
     CDS             complement(5761..6366)
                     /locus_tag="LNE23_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6932416.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2109 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaatttt
//
LOCUS       DAFCNS010000109         6307 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFCNS010000109 DAFCNS010000000
VERSION     DAFCNS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.112"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            42..197
                     /locus_tag="LNE23_002606"
     CDS             42..197
                     /locus_tag="LNE23_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932417.1"
                     /translation="MVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLLNL
                     FNRIVGA"
     gene            210..710
                     /locus_tag="LNE23_002607"
     CDS             210..710
                     /locus_tag="LNE23_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK6932418.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            807..2654
                     /locus_tag="LNE23_002608"
     CDS             807..2654
                     /locus_tag="LNE23_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBK6932419.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2657..3553
                     /locus_tag="LNE23_002609"
     CDS             2657..3553
                     /locus_tag="LNE23_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6932420.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3603..3992
                     /locus_tag="LNE23_002610"
     CDS             3603..3992
                     /locus_tag="LNE23_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6932421.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3979..>6307
                     /locus_tag="LNE23_002611"
     CDS             3979..>6307
                     /locus_tag="LNE23_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6932422.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2124 a          936 c         1351 g         1896 t
ORIGIN      
        1 aatggttttt aaccaatgct caatctttag tcttactagc gattgtggtc attggtttgt
       61 ttttaggatt taaacgcgag ttttccaaac tgattggctt cttggtgatt gctttgattg
      121 cggtaggtct ggtctttaat gcttctggtg tgaaggatgt cttactcaat ttgtttaacc
      181 gaattgtcgg tgcctgaggg ggaatcacaa tggaacagat acgtgtttat attgcgaacc
      241 ttggaaaata caatgaaggt gagttggtgg gggcatggtt taccccaccc gtcgattttg
      301 atgaagtgaa agaacggatt ggattaaatg acgaatatga agagtacgca attcatgatt
      361 atgaactgcc ttttgagatt gatgagtata caccaattga ggaaattaat cggttgtgtg
      421 gtttagcgga agagcttaaa ggaacgccaa ttggtgaggt cgcttcagag attcaacatg
      481 ccttcttcaa ttctttcgag gaaatggtag agcatgtgga tgatatcatc tattatcccg
      541 attgtaatga catgagtgat gtagcgtatt acttgattgt agaagctggt gatttaggcg
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtcga gacttggaga
      661 ttggcgggaa ttatttggtt acgagtcgag gggtttttga actaatgtaa atcatttgtt
      721 agaattgacc agaaatttct atcgtacttt tacttttcta tttgatatac tgaaatagaa
      781 aatatttgtt gataggggtg ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc
      841 atattcattc aactgcgagt cagtgctttc aggataaaaa ggttactgct aaggaatggg
      901 tagaagaatg tttaaacaaa ggactcgatt gtgttgcatt aactgatcac aatacagggg
      961 aaaatattga tgaatataag aaagtggcta tcgaaaatgg cttagatttt tttcctggag
     1021 tggaattaac gtgtggagaa aatggaactc atttactagt cctatttgat aaaagtgcat
     1081 gtacaaatga tatctctgat ttcttaacca agatgggtat aaaaagagag gattgggcca
     1141 gcgctgcagc ttgctcaatg aaatcagttt tagaagttat tgattttgct gagcacgagg
     1201 gcttgctcgt tattcctgca catatagatg agtttaatgg aatttcttca gcaaaatttg
     1261 gaaatctagg aaagatattt gaatcagaaa atatatatgc agtacagacg gttcaaaaag
     1321 aattttttga aaatcgctca caaatgattc cctcatctaa gcgtgacact atctatgata
     1381 gtgtgaatgc cagatatgat ggtagagttg gaaaagatac tttggaatct tggtataaat
     1441 cagttgttga ggcagaaaaa aataatatga catttcttag cttttctgat aacccacact
     1501 cgaaaggaaa ttcaaaacat ggactttggg gaataggcac aagatattcc tatataaaaa
     1561 tgaaggacga acctgacttg ggatcacttc gcgatgcttt aatgctaggc gaaactcgag
     1621 ttcattctga tttttctaat ttctccatta atgaaaatga agttttattg gaaaaattaa
     1681 gcttttctgg gaccactctt agcacaaaag aagttgtagt cgaattcagc gataatttaa
     1741 cttcaattat tggaggaaga ggaacaggca aatcttgtat tacaagattc ttggtctatg
     1801 tccttggaaa ggaagcagaa cttgatcaat tttctgaaat acaaagtgat tatcagaact
     1861 ttgcacaaat tgaacataat ggctctggaa tatttttgaa ggatacaatt gtgaaactga
     1921 atattttcta taaggggaca aaatatcaaa taataagaac acaggataga cattctattt
     1981 atgaatttac acaagagaat ggtctggttg aaaaagaaac agaacggttg agaatgattt
     2041 ccgataaggt gagtatatac tctcaaaaac aaatttatga aatttcaaaa aatcaaacta
     2101 gtatactaga attactagat ggatataact ctgatttgat ttcagagtat aaaaatgaga
     2161 ttgaaacatg tgtaaacgaa ataagaaaat taaattggga tattgtttct gtgaaaaaag
     2221 aaatacaaga taaggcgaag gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa
     2281 agctatctca taagagttat aaagatgttt atgatgagta ttcaaaagag acagatattt
     2341 atagggaact gaaacgtgat gtggaggcac ttaaggaaat tcccaaaaat ctaactgata
     2401 gtgttgacaa aattgacttt ggcaatggta taaaagttac agacgaaatt gatgaacacc
     2461 gcggagaatt gattaagaat ttagtgacca atcgggctaa gattcaaaca ataattaatg
     2521 aaatgtcaga tgaaattgat agttatagag cagagctgaa taaatcagat tggaaagtta
     2581 actataatga ggtttcaaag aggtatcaaa gaggtatcaa aaacttagtg aagagttatc
     2641 tcaagatgaa ttaaaaatgt tacaaaacct tgattcttta tatcaatctt tatcaaaaac
     2701 tgaagagcga ttggaagaaa ttattcaaca agaaataaag cttaaagatt accaagttga
     2761 ggttgactca gcaatgtcaa gaatgaataa atcatatcaa aatctctatt ctgagagaag
     2821 tagttcctcg cgcgaaattt tttctgatat tacaggaata gaggctacta ttcaaccaaa
     2881 tagggatttc aataattatg ttaaagagct cagagcttgt attaataagg aggacacttt
     2941 tgacagtgag tttggatctc ttattgataa gctgacaaaa aattccatta ctaatcaaga
     3001 tatatataat gacatacttg atataagagt tagtggggaa agtagtattt tttcagataa
     3061 aaaattaatc aattctataa gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct
     3121 taagccaaat gatttagtag ttatccgact tgaactaaat ggaaaaaaag ttagtttatc
     3181 gaatgctagt gctggtcaaa agacatctgc aattctttct atgattttgg cgtatggaaa
     3241 ttcaccactc atattggatc agcctgaaga tgatttggat agtcaattaa ttaataactt
     3301 aatcgtaaga agtatcatta caaaaaagga aaacagacaa attttaattg taactcataa
     3361 tgcaaatatc ccagttaatg gtgattcaga atggttgatt agcatgggag attcaaaaga
     3421 aatatcggct gatcagtgcg ggtcggtaga tgaacccaat atcaagccaa gaatatgtac
     3481 tgtgatggaa ggtggcgagg atgcttttac aaatagagcg aaacgctatg gttttaaaca
     3541 aattgtagaa taacactaga gggtgagaca tattattatg tccgcccttt ttaggaggaa
     3601 aaatgaaaaa aataaaaagc tataccagta tctgggcagt tgaaaaagtc atttacgcaa
     3661 tcaatgattt tcagttgcct tttcctgtga cttttaacca aatggcttgg ttcgttttat
     3721 ctttactgtt tgtcatcgta tttgcccatg tgcctccact atctatgata gaggaggtct
     3781 ttttaaaata cttaggcata ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg
     3841 atggcaagaa accactggga tttttacgat cctttatcag ctatcacttg cgatttaagg
     3901 tgacttttgc aggaaagaaa gtcaaagaac agaagaaacg atgggatgaa cccagtactc
     3961 ttgtaaggag tgtgaactat gtatccgatt aagtatattg aaaataatct cgtctttaac
     4021 caagaaggcg agtgttttgc ctactatgaa ttagtgcctt acaattactc ctttttgtca
     4081 cccgaacaga aatttcaagt gcatgataac tttcgccaac taattgcgca aaatcgtgaa
     4141 ggcaagatcc atgcgttaca aattgccaca gaaagtagta ttcgcgccac acaagaacga
     4201 tcaaaaaaag aaattaccgg tagactcaaa gaagtagcga aacaacgaat tgatatacag
     4261 acagatgcat tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc
     4321 tttaaactaa ttgcgacaga tgaagaagtc aatctaaaga gtctcaagaa atctttcttt
     4381 tctgggcttc aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc
     4441 ttatccaatg aagaaattcg ctgctattct aagcttgaaa aattaatgga aagcaaattg
     4501 gctcgaagat ttaaagtcag gcgagtcaca ccgagtgacc tgacgtattt gattgaacat
     4561 atttatggcg aaaaagggat accgtttgaa gaatacgaat ttcagctacc aaagaaaaag
     4621 ctaaaatcag aaacactggt gaaacgttat gacttattac gtccaaatcg ttgtttaatt
     4681 gaagaaaaac caaggtgctt acggatggaa catgagaatc atgagtcgta cgtagcgtat
     4741 ttaacaatta atacaattgt gggggagatg gaatttcctt cctcagaact tttctattac
     4801 cagcaacaac aatttacatt cccaatcgac acaagtatga atgtagagat tgtgacgaat
     4861 aaaaaggctt tagctaccgt tcgtaataag aaaaaagaac tgaaagattt agacaatcat
     4921 gcttaccaat ctgacaatga aacgaattcc aatgtcttag atgctttgga ttccgtggat
     4981 gaattggaaa ccaccttgga tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt
     5041 cgagtgagtg ccgaatcagt ggatgaacta aagcgacgct gtgatgaagt gctcgatttt
     5101 tatgatgata ccaatgtaaa actagtccgg ccctttggcg atatgatggg gctacatgaa
     5161 gaattcttac cctcaagtaa acgatatatg aacgactata ttcagtatgt tacgtctgat
     5221 tttttggcgg ggcttggttt tggggctact cagatgctag gagaattaga agggatttac
     5281 tttggctaca atgtggatac aggtcgtaat gtttacctga aaccggcatt ggcttcacaa
     5341 ggagtcaagg gttcagtaac caatgcgctg gccgccgctt tcctgggttc tcttggtgga
     5401 ggtaaatcct ttagtaacaa tctcttagtc tactatgctg ttttgttcgg tggtcaagct
     5461 gtgattgtcg atccaaaagg ggagcgagga gggtggaaag aaaccttgcc agaaattgcg
     5521 gatgaaatca gtatcctcaa cttgacgagt aagtcagaaa atcaaggtct gcttgatcct
     5581 tatgtgatta tgaagaaaaa gaaagactcg gaaagtttag caattgatat tctgactttt
     5641 ttaacaggaa tttcaagtcg agacggggag aagtttcctg tgttacgtaa agccattcga
     5701 acggtgactc aaagtgatca acgggggtta ttacaagtta ttaacgaatt gcgaaaagac
     5761 gggtcaccaa tcgcggaaaa tatcgccgat catatcgagt cctttaccga ttatgatttt
     5821 gctcatttgc ttttctcaga cggctcgatt acccaatcga ttagtttaga taaacagcta
     5881 aatatcattc aagtggcgga tttggtttta ccggatgcag aaacgacttt tgaagaatat
     5941 accaccatgg aattgcttag tgtttcgatg ctaatcgtca tttccacttt tgccttggac
     6001 tttatccata gtaaccgaga aatcttcaaa attgtcgatt tggatgaggc ttggagtttt
     6061 cttcaagtgg ctcaaggaaa aacgttatcg aataagcttg ttcgtgctgg tcgggccatg
     6121 aatgctgggg tctatttcgt cactcaaaat agcgacgatt tattagatga acgtttaaaa
     6181 aataatatcg ggttgaagtt tgcttttcgc agtaccgata ttcatgaaat caagaaaacc
     6241 ttggaattct ttggtttgga tcaggaggat caaagcaatc aaaaacgcct gcgagatttg
     6301 gaaaacg
//
LOCUS       DAFCNS010000110         6239 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFCNS010000110 DAFCNS010000000
VERSION     DAFCNS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.113"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(316..603)
                     /locus_tag="LNE23_002612"
     CDS             complement(316..603)
                     /locus_tag="LNE23_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6932423.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(623..847)
                     /locus_tag="LNE23_002613"
     CDS             complement(623..847)
                     /locus_tag="LNE23_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBK6932424.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1302..1472)
                     /locus_tag="LNE23_002614"
     CDS             complement(1302..1472)
                     /locus_tag="LNE23_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932425.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1487..2020)
                     /locus_tag="LNE23_002615"
     CDS             complement(1487..2020)
                     /locus_tag="LNE23_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK6932426.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(2041..2985)
                     /locus_tag="LNE23_002616"
     CDS             complement(2041..2985)
                     /locus_tag="LNE23_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBK6932427.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3622..3708
                     /locus_tag="LNE23_002617"
     CDS             3622..3708
                     /locus_tag="LNE23_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBK6932428.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3829..4518)
                     /locus_tag="LNE23_002618"
     CDS             complement(3829..4518)
                     /locus_tag="LNE23_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932429.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4525..4803)
                     /locus_tag="LNE23_002619"
     CDS             complement(4525..4803)
                     /locus_tag="LNE23_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932430.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4763..5677)
                     /locus_tag="LNE23_002620"
     CDS             complement(4763..5677)
                     /locus_tag="LNE23_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK6932431.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5659..6039)
                     /locus_tag="LNE23_002621"
     CDS             complement(5659..6039)
                     /locus_tag="LNE23_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBK6932432.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1651 a         1173 c         1053 g         2362 t
ORIGIN      
        1 cgtatttggt gcaaataacc gcttgtcaaa ggggcgcaag ctcccccttg aatccccctc
       61 cccccccact ctccacaata caaaaaagac tagcaaatgc tagccacttt ttgtaaagtt
      121 tcgcttgttt caccttcggt gaaattgttg caaatcaata attaggtaga gctacatttt
      181 tttcgttgtt caaagattct tatgccattt actattaata aatctataaa tatgataatt
      241 tttcggctct ataatttatg ggactgatga atcagaattg attcttcagt cccattttca
      301 ttttattcca aagaattaac tccaaatacc aatagaagcc catcctctat atacttgaga
      361 ttccttacta acaatttctt gtaattctat taaagattta ggtaatccaa atttatgttt
      421 taaaatatat cttccaagaa aactacttaa tttcatacta acttcaccaa cactttttcc
      481 ctcactgatt tctttagaat aaagaaagag cttttctttt aagtcaggat agtcatctat
      541 agttgcttca ttgtatactt tactaattgc atttaaaagt ttttcttctt tagtaaaatc
      601 cattataata acctccctac cactaccttc taggtgccca tggaccatga ttaacccaac
      661 cattaacaat aatttttcca atttctcctt tagcttgatt ccaatctacc caacattttt
      721 ctttgttaca ataaagacca tttccatagt aagtagctgc atcaactttt attcctgtac
      781 cgatgccagc tagacaagtt cctaatattc ctagaataac acaatgtttt aatactttct
      841 ttttcatgat tgtttttcct tttcgttttt ttaaaattgc ttgttatatt aaataatctt
      901 agtaatttta ttatataaca ttttattata taacaatgat taacgtcata aaaagtagtt
      961 ttgaattcat aatcgtttaa ataaaattat aagatcagtt atttaaacag ctagtagaca
     1021 taatgtatat actaccaagt aacgcctgta ccaagggatc caggcgctga ttttgtgaat
     1081 ttttattggt ctaggttaaa agctcttttt aaggcttggt tttctgcggt tgtttctttc
     1141 taattctttg tgaatattga tcggtcattt tttaaatact tgttattttt gtttgtgttt
     1201 gttatgcttt ttacatatct catattcatt tattattctt ttctaaggtt aataatagga
     1261 taatctcttt cagaaagact gctcgtacga gcagtctttt cttatttatc aagtttttct
     1321 acaaatactt cctcattttg actgttgtag taattaggat tgagttttag ttcccctttt
     1381 ttatttcctt tgttggccac tgataataac attgggctaa agcttaatat tcctagtagg
     1441 atagaaccga atagcaagtg tttttttgtc ataagtattt ccttcttcat ttccatagct
     1501 gccaccattt tttccaattc tttgtttgtt cgtccattgt ttctgttgct tcagaaagag
     1561 tcgttgaact attctctgaa gactcttccg tataagtcaa ctgcagttgc tcctgaagtt
     1621 tttcgatttg ttggttggct tgcagggtga gttgttgttg ctggtcgagt agtttttgta
     1681 tatgagaatt attttgctct ttgagggcaa gttgttctcg taaaaattca ttttcttcac
     1741 gcaactgatc tagcaacttg tttgcaacca cgtttttgtt tggtatataa gcatttttat
     1801 cgttgttttg ttcatgttta tgcaacttat ctagcaacct ttgattgtct aagttgcgag
     1861 caagttgcaa tactccagaa cgcaatacaa ccttataacc acgctcattt ttagctatca
     1921 tggaagaatc taatttttgg atatgtttcg atattgcttg cctactcatc ttaaaaagat
     1981 ctgacaattc ctttattgta aaggtttctt gtggttgcat actggttgcc tcctaggttg
     2041 ctactggaat aaattatcta aatcagataa taaacttttt ttctcttttt ccgaaagtgc
     2101 ggactctgtt tgtttttcaa gagctttttt ctctttttct agttgtttga tttgttggag
     2161 gagttcaagt tctcgtttct tctgtttgat tttttcgtta attgttgtag cgggttcttc
     2221 gtattctttt tgtttatcag ctaaagccaa tgctttattt tggtcttctt tggttggtgc
     2281 atgtttgtta aagtcgtctg cttttttgat ctcaggtttg aacgtaaatt gatagccaat
     2341 aacaggtttt ccccgtcctt ttccgtattt ctttttgatt gttagtcctt tgaagattgc
     2401 agttagttct tgacgtatag gttttataac tcgtttatct atatcaggtt gcttataact
     2461 tttaggaata tctaataatt caagaaagtc ttctttggag aaatatgcga aacctttggt
     2521 tctgtattgc tttaacagac gaaacatagt ctttgcatag ccactttgta gctcgttgaa
     2581 ttgttgtaaa gaatagcgta cccactcgtc taaattattt aacaaaggca gtgctttttc
     2641 gtaaattcgt atatctacgt aaggattttc tacgtccccg ttgattttaa attctgtaaa
     2701 cattacaaac atatcgtaag aaagcccaga ctcacttcta cgaccaaagc gtaaagataa
     2761 gattttttca taagttgctt ttaaatcttc tacaaatcgt acatttgcag ttgctttgta
     2821 gttacttaaa tcttttagtt ggtcaaacgt aaaacgaact gtcttatccc ctttttcacg
     2881 cattctagac acgatggaaa aaaacaaatt catttcaacc ggtgtaaatt ttcttaaagg
     2941 aattgtattt aattcatggt ggtattttac aatctcatta gccatataat cacctcaatc
     3001 caattatgaa tgacattttt taaatgtcaa gacattcata tttgacgttc aaacagacaa
     3061 aatgtccttc ataaacagac aaaatgtcct tcataaacag acaaaatgtc cttcataaac
     3121 agacaaaatg tccttcataa cattctgtat ccattggtag agtaaggaca cagcttacgg
     3181 taaagaggtt taaaaagagg tttaaaaaga gatataaaaa gaggggggct aattttcccg
     3241 cctaaaaacc acttcttttt tctttaaaca ggttcgcgca tttttcaaaa ctgccaatcc
     3301 aaaaccttgc ttcctacggt cgccaaaaag gtgtgatcgc tacgctctca cattgtactg
     3361 cgcagtcgcc taaaggctcc tttgctaaaa acatgttggt aaagaggcct ttgagcggtt
     3421 ctgaatgctt atctaagccg ttttaagaca gtcttggata aaaatcctta tctagcttta
     3481 aagcgcctta caggcggtca gggcaagccc taacaaccct aaaataagcg atacttaaaa
     3541 acaccacaca agtttaattt tttaagatat aataacttgt cgcctatccg acagtcttgc
     3601 gactggaagg aggtgtatct aatgtttgaa ttgttcattt gcccccttct tatagaagtt
     3661 tgtgtctctt tattcagtta ttggttgaat aaccgaaaca agaagtaaaa tcaataaagg
     3721 cgacatcaac ctgaaaaaaa gccccagaga atccaacctc tctggggctt tcgtgtatct
     3781 aatgtttgat catttgcatg tttagtttaa catgattgcc gatcaaattc aattttgctg
     3841 ttgctgatct aaccattcct tgacactttt tagataattt aatgccgttt cttttttcgt
     3901 tggattttgt atgttcgttg cttgctgaag cagtgcttta taacgttcgt aggtaactga
     3961 ttgttgccgt tcttttcgtt cctgttcact agttgaaaaa gggattgtat cactgaataa
     4021 attataacaa gcttcttctg tctgcgtatc tttaaaatat tgcacgtctt ttttggttcg
     4081 ctcatagttg tcttgaacct ctttttgctg tttttctggc aagttttgat aaagacgagt
     4141 gagtttttcc atgtttttat ccgtcaaacg tttttcttga ttcacaaaaa tatcgttcat
     4201 ttcctcataa caagtggtaa actcttccat tttttgtgtt aacacttgct cctttttctt
     4261 tttatcacat gctgcttcat acttttgagt gatatcccct aataaaatac ttcttgaaat
     4321 acgtttattt ccctctaaaa cgccgaataa accacttaaa acgattgata atgtaaatat
     4381 aacccctggc aataaaaacc tcttaaatcg aattttagag ccttttaatg aatatcttgt
     4441 ttgataagcc ttatagctaa gccaaataat tcctaaaata ctggcaaaaa tgagtagata
     4501 gggtaaatag aatgccattt ctatttattc tccccatacc agccaaaagg ctaacagtgt
     4561 catgattaag caacataagg gggcaagaat atccattgat cgctgcataa ttactttttt
     4621 ctgttcttta tttcgctggt ttaatagcgc atggtggaat tttttgactt gtgaatcaat
     4681 tgtagtttgt attctttctt tggtataagg tgcaagaaaa gcacgttgtt tctttatttc
     4741 attttctatg tcttgtaacg ttttagaacg ataattcttg ttggtagtct gctcctcttt
     4801 caacgtgttg gtcatggttt cttcgagtcg gtctaatttc cggttgttca gtcgtaagag
     4861 ttcgttgttc tcttcgtact tcttttgcag tttcgcgaat gctagctcga gctcttcgta
     4921 gctcgttggt tcgttgttga ttgtaggctc gttctcgtct gtccagctca tgtaaaatcg
     4981 tctccttttc gtaatccttt cctagtttgt tggatcgtac ttttttcttt ttgtggatca
     5041 attcgtaagt aaaatttttc ccacgattca agcaatgaat gccgaatgtt ttaagagaat
     5101 cacaaaattg gttatacgtt cgagtttttt tgtttttcat tgtttgatca atcgcaaacc
     5161 gaatttcgtc tttccaaaca ggttcatttt tttcttttaa tttgatttca gacatggttc
     5221 gtttttcggg tttcttccct cgttcagtga ttgttaagtc atgttcaaga caaatctcgt
     5281 catttaggtc ataacatttg tttaaaaatg cttgaccatg ggcttgaaat ttgttcccag
     5341 tttccagatt gacggagtta atgacaatgt ggttatgaat atggtctttg tctgtatgtg
     5401 tgtaaatcgc gatttggtgg tcaggggcta tttttttcgc atactctgcc cctagttgat
     5461 tggctttttc agcggtaatt tctcctggtc gaaaagactg gatcaataag tgtgcttgta
     5521 cacgatcgtc ttttccatac agcatgcgaa tttgtttcat ttcgcttttg gcataatcaa
     5581 tgtcacagtt tatccctgtt ttgacggttg ctcgtggttc ggcgtaattg atcgctcggc
     5641 tacaagaagt ggttcggctt actttaactg ttgccatatc ttttgtagct ccttttgcat
     5701 gatttttagg ttttcggata agcgttgggc gtcgccagga tcaacgtcgt gttggtgtgt
     5761 gttgcaccac ttggcaattt gattgatatt cccgccagct ttggctaaat cgcgcgcgat
     5821 tgcctgagca atgtctggcg ccactttcgg tgtcaccaga cgcgcgtttt gtgccttggc
     5881 tttcacgaaa gccggcacac tcatttgcaa agcttcagct gactgcttca gctttgcaaa
     5941 ttcggattcg ctcactcgaa aactgatttg ttttggctcg gatcgatagg tatatttttt
     6001 agaatgattg ctagccaaat attgattttg ttcgctcata agtcccaccc tttcagtacg
     6061 tcctacggac gtatcgttcg caataccatt gtacctaaaa aggtacaata agtaaagctc
     6121 actaaagctt actaaagggc ttggcaagct ctatatttgc tagccaaata cctccgtatt
     6181 tggtgcaaat aaccgcttgt caaaggggcg caagctcccc cttgaatccc cctcaaaac
//
LOCUS       DAFCNS010000111         6023 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFCNS010000111 DAFCNS010000000
VERSION     DAFCNS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.114"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(64..366)
                     /locus_tag="LNE23_002622"
     CDS             complement(64..366)
                     /locus_tag="LNE23_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932433.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(586..952)
                     /gene="ssrA"
                     /locus_tag="LNE23_002623"
     tmRNA           complement(586..952)
                     /gene="ssrA"
                     /locus_tag="LNE23_002623"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1123..1896)
                     /locus_tag="LNE23_002624"
     CDS             complement(1123..1896)
                     /locus_tag="LNE23_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6932434.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2040..2390
                     /locus_tag="LNE23_002625"
     CDS             2040..2390
                     /locus_tag="LNE23_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBK6932435.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2371..3087
                     /locus_tag="LNE23_002626"
     CDS             2371..3087
                     /locus_tag="LNE23_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6932436.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3087..4535
                     /locus_tag="LNE23_002627"
     CDS             3087..4535
                     /locus_tag="LNE23_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6932437.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4606..5115)
                     /locus_tag="LNE23_002628"
     CDS             complement(4606..5115)
                     /locus_tag="LNE23_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932438.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
BASE COUNT         2047 a         1050 c         1170 g         1756 t
ORIGIN      
        1 atatcccttt tataagtatg gtgataaaat ccgttccggg ctaccttttt taggtagcct
       61 actttaatct ttataccttt ctggatcaac gaaagtatac ttcaaatagt cataacgccg
      121 atgatcgctc cgtgcgtctg gcacatcagt cacgatatca aacaaaaaat acacatcttt
      181 cttcattctg gtttttgttg ccggaatttt aaagtagttc ttattagagt aatagagatt
      241 gattaataag ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca ccttataaaa
      301 atctttgaca aatctattcg aaggatcaaa tttaaaccat aattgtgttt ttccttccat
      361 caacataatg ttcacctcaa aaagagtata cgaactaatg ttctttttgt aaagtaggaa
      421 tatcattgat aatatggatg gaacatatat attaaaaagt gttctttttg aatgctattt
      481 tgaatgccat tttcattcat ttaacgaaaa tctgcgaaaa taaaaaatgc tcaaaccctt
      541 ataaaataag gatttaagca tctagcgaaa tctgctgaaa tctcacaatg gagacggcgg
      601 gagtcgaacc cgcgtccaaa cacatcgcca cttaaaaatc tacgttcata gtcaactcat
      661 ttaaggattc actttacagc ttgccgagtg acaggcattc tgtatcgcta gtctgatgtt
      721 ctctttttca acttacagac ggaaagttga aacgtatccc actaaagtta ggacccttac
      781 tcgagcacat gggcgatgcc gagaggatct acgctaactg tttttaggca gctaaagcgt
      841 aagagttgtt ttcgttttta gcagttatat ttaactgtaa cgtttttacg tagacgtaac
      901 ctacgaaacg caattcaagc tcgactgtgc ctgtcgaatc cgtaacgtcc ccgttagcta
      961 acaagggtta cgtgtatttt tcatacaaaa ttagtatagc atatattggg ataaattcaa
     1021 taaaaaagcg aatcatatcc cattatttat ataataactg cccctttctt aagaaaaaat
     1081 aagcgatttt cttaagaaaa gaggtagctt caaaaaaaga tcttaaatca gatcaaaata
     1141 cttcaatgcg tggacaattc cgccttctgt atttttcttt gtgataaagg tagaaagttc
     1201 ttttaatccg tcataggcgt tgcccatagc gattttgttg tcgcaagctt ccaacaaggc
     1261 aaaatcattc ggaccatcgc caaatccaaa agtaggaact ttttccagct gaagttcttt
     1321 ttgcagacgt ttgacacctg ccccttttga gattcctttc ttcactgtat cgatcgagta
     1381 aggaccattc cgataaaaag ttagctcagg aaaatgttcg tgatagtatt cgtcacctga
     1441 ttgagataag atcagcaaca tatttactgt gttttcttta aagcctaaag gatcaatttc
     1501 cggaacatct gaatgaatga agtcgtaagc attgatcatt gtttggctat gaccggtaca
     1561 ccagattttc tgatcgttat agtaagaaag ttcatgaccg ttcaactgta catgatcata
     1621 cattttttga cattcttctg aagaaaatgt ttgagaatag atttcttcgc cgtctactcg
     1681 aatgaaagaa ccattcatgg tgaccgcaga agtaatgccc gcatcttcca tgatagagtg
     1741 gatttctgct tctgtacgtc ctgtagcgat gacggggaga acattgtttt cttttaatgc
     1801 gttcatagct tttgccactt cgggtgtgat ttttgatttc gcatctagta atgttccatc
     1861 aagatcaaag aaagtgattg cacgataatt tgacacaaat aaaactcctt ttttattgga
     1921 ataagtttat cttaacaaag aatgaacaaa agacaaacct ttcgtgaaga attcctgaac
     1981 atattggttc agtcaacaat ttagagtata ctataagaag agaatttagg agggaaagaa
     2041 tgattttttt cagtattggt atcttattct taataattgg cagtcttttt cggattctgc
     2101 cctcaaaagg aaatctgcct ttttatgggt atcattcgcc tcttgccgca aaaacagacg
     2161 ctcattggcg gctggctcaa aagacaagcg gaaactggtt ttttcttatg ggcttactga
     2221 tggcattgat cggctattat ttaaagacat ctgggcatac gaattatttt ctgattgaaa
     2281 tgcttctgct agtatttcca attatgccta tttttattat gacagaaaag aaattacaga
     2341 aatatgacct ggaaactgga gggaacgata atgaatattt taatgattga agataatcaa
     2401 tctgtttctg aaatgatgca gatgtttttc ttaaatgaag gttgggaagc aactttcaag
     2461 tatgatggaa aagaagggct cgatgccttt ttagaaaacc cacaacattg ggatatgatt
     2521 acactagacc tgaatttgcc cacactggac ggtatgctag taggaagaga gatccgcaaa
     2581 gtttcaagta ctgtgccgat tatcatgctg acagctagag attctgaaag cgatcaggtg
     2641 atcggcttag agatgggggc agatgattat gtcacaaaac ctttcagtcc tctgacgctt
     2701 attgctcgaa tgaaagcttt gcatcgccgt agtgagttgg cagaagcaag agaagaagca
     2761 gtagtttcag atgatcattt tgatatcgag actagtcatt tcaagatgaa tacgaaaaca
     2821 cgagaagcgt atctggacaa ccggccaatt gaaggactga cgcctaaaga gttcgatcta
     2881 ttgtttactt tagcaaaaaa accacgtcaa gtattctctc gggagcagtt gcttgaatta
     2941 gtctgggatt atcagtactt tggtgatgaa cggacagtgg acgcccatat caagaaatta
     3001 cgacaaaaaa tcgaaaaggt cggaccacaa gtcatccaaa ctgtttgggg agtcggctac
     3061 aaattcgatg attctggtgt tgcctaatgc gttatttgta tcagcaactc ttagctttct
     3121 gcggaatggt cgcgttgatc ttattgatcg ttggagtttc ttttacacaa ctgacaaaac
     3181 aaacgattga agaaaacaat taccagcaac tgtttggata tgctgaatca gttgaaaaga
     3241 caacgcaaac ctatgccgat acgctcccgc agtttagtca agatcaggcg tttcaaaatg
     3301 cattgctgct gacagagcaa gtactgacag aacaaaatgt gaactttata tttatcgata
     3361 aaaacgaacg tgtcatttat ccgactaata cagctttact gaattttacc ttaacagctg
     3421 atcaatggaa aaatctgagg aatggacgtc aagaaaaatt cacatcgaat aaaaatattt
     3481 taggacaagc acaggcgacc tcttacgcac ttgtgccatt taatttgaat catgaattct
     3541 atggagcact tgtagttagt caacctgcgc gaaatatcga taacagcgtt cgtccaatta
     3601 cactgaacct gttcaaagga tttatttttt ccagtatcgt tgcagttatt gccagctatg
     3661 cttttgcagc attccaagtt aagcgtatca atcggttaag gaatgcgacg aaagaagtga
     3721 cgaatggtaa ttttgatgta cagctccccg tacatgacaa agatgaattt gatgagttag
     3781 cagatgattt caataaaatg acgaattcat tgaaagaatc tcaagcagaa attgaagaac
     3841 aagaaaatcg tcgaagacaa tttatggcag atgcttctca cgagatgaga acacctctta
     3901 cgacaatcaa cggacttctg gaagggttag aatacaatgc aatccctgaa aatcagcgag
     3961 aaaacgcgat taaattgatg aaaaatgaaa cagagcgtct gatccgtttg gtgaatgaaa
     4021 acttggatta tgaaaaaatc cgtacgaatc aaatcagtat ggtcattaaa aaattcaatg
     4081 gaacagaaac attgaaaaat attgttgcac agttagaagc taaagcagaa gcagcaggag
     4141 atacattgac attgaaagcc gttgatgata ttgatgttta cgctgattat gaccgatttg
     4201 tccaaatcat ggtcaatatt attcaaaacg cgatacagtt tactgaaaac ggccaaatca
     4261 cgattacttt agaaaaaggc tatcttgaaa cgatcatcac gattgaagat acgggaattg
     4321 gaatgtctga acaacaaatg aaaagtatct gggatcgtta ttataaagta gatccttcaa
     4381 gaaaaaatac aaaatatggt gaatcaggat taggtttacc aatcgtgcag caacttgtac
     4441 gcttgcataa aggaaaatta gaagtagaaa gcgaactagg aaaaggaaca aaattcgctg
     4501 ttcgtttgcc tgacgtagaa atcaaaaaag agtaaaaaac aagcggtata aaagatcatg
     4561 aatcgatctt ctataccgct tgttttttac tataaaatga ttgatttagc ccgcgggctc
     4621 taaacgatca atataaggac tttgagcaat agtagcatca tgagtaaaga gtaagatacc
     4681 ttgattttct tcatgagcta aataagaaag caagtggagt agctcttgga tctccaatac
     4741 actaagttca tcaaatgtat cttcaataac aatattgttt cgcttggaaa gcaaggcatg
     4801 aagtaattgc agctttactt tttcaaaaga cgtcaactca gctgccgcac aattaagcaa
     4861 ggaaggagag atatcaagtt tttcttgata ggccaaacga tcttgtttga tttctttttc
     4921 cggtacatcc aacaggacat ggtctttaag attcagataa ggaaacatcg gccaattttt
     4981 ttggatgatc gcaaaattat ccagttttgc aacaagaacc tcttttttca agtgctccaa
     5041 tattttatgc tgtttttcga gaggacacaa aataacggtc aagtgttcag catgaatcaa
     5101 atattcaggt ttcattacgt ccaggcctcc tactttgcaa atgatggata aaaaagtgaa
     5161 agggaataga ctaagaatga gacagcatcc actgagccca ataagagttt gggcaaatcc
     5221 cttaagtata tctgtgaaaa aaagatcatt ttggaaatcg atggtaaaaa aagtctgttg
     5281 atccatcgga attgtaagtc cgcttaccga agtcaagata tcacggatat ctggcagttc
     5341 agtagtcttt aagacaagaa aattgatcct ttgaagaagc gcttcataaa ctgactggaa
     5401 gcttacgatc cacaataaaa ccaagataca acaaagaaag agaggaaaga acagctgcca
     5461 gaaaataaaa aggaataatt tggttttaga aagaccagtc aggtgccaat tgatcaattc
     5521 ctgcttgttt tgcctgactg aatagtaaaa gaaaaagata aaaaggaagg caaatagaaa
     5581 aaataaactg aatatgcctt ttatataaaa tcggctagct aattctaaag ccggaagaag
     5641 ctctgtagat gcagaagcgc caataaatga aagccgatct tggacttgat tggctaaagc
     5701 cgattgcaaa tggtaaagag tcaggcatgc tatcaataaa aaagcagaaa agcttgaaaa
     5761 taaacaatag agtattgtct gctttttgta ataaattaat cgagcagtag catagcggat
     5821 tattttcata cttttcacct ctataaaaag tatagaaaat gaatatgaat aaagtatgaa
     5881 attcctttag tgaaatcacg aattaaaacc agaaaatatt ttgtaaaaag caagaggttg
     5941 tgaagaagct gccctattct attaccgtag atgcaagctt ttgaacactg tttattcgaa
     6001 aagaggagca gcgacaataa ttt
//
LOCUS       DAFCNS010000112         5889 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFCNS010000112 DAFCNS010000000
VERSION     DAFCNS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.115"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(74..1168)
                     /locus_tag="LNE23_002629"
     CDS             complement(74..1168)
                     /locus_tag="LNE23_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK6932439.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1357..2118
                     /locus_tag="LNE23_002630"
     CDS             1357..2118
                     /locus_tag="LNE23_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6932440.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="LNE23_002631"
     CDS             complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="LNE23_002631"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK6932441.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2893..3648
                     /gene="fabI"
                     /locus_tag="LNE23_002632"
     CDS             2893..3648
                     /gene="fabI"
                     /locus_tag="LNE23_002632"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBK6932442.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3758..4771)
                     /locus_tag="LNE23_002633"
     CDS             complement(3758..4771)
                     /locus_tag="LNE23_002633"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBK6932443.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4936..5856
                     /locus_tag="LNE23_002634"
     CDS             4936..5856
                     /locus_tag="LNE23_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBK6932444.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1793 a         1174 c         1054 g         1868 t
ORIGIN      
        1 tataaaaaaa caaaataaaa aagcagaaat aactggctat ttctagcgga ttatttctgc
       61 aataactaat tatctatttt ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa
      121 agtcaaggag gaataattcg tcatcggaga ttcttcctac aacattgaca cgtccgtcgt
      181 ttttcatatc cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac ccttcattgt
      241 caatcagtac atccccatga tgcatatctc gcgtattatg aggcgggaaa tctttatctt
      301 tgtagtatac acgagtcatc gttgaacgaa ggatatattc tgagcgatcc cctcgataac
      361 tatgaaggtt atcaaacaag cagattcgtt cgttttctgt aataccttct tccgtatcta
      421 ctttcaatgt tggataatcc gcgttaaatg cttctgccat ttctttcaat tcttcttcac
      481 ttgcgtacgc atttccaata gaaatatcat caatcaatcc tgtaaggatt aaatgcttca
      541 cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt gcacaagcca tcttgcgtag
      601 gccaagggcc aaactcagca gattgcgaat tgacgaatgc catcgtattc aaattatatt
      661 ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag tatcgatgag
      721 gataaaaatt atgagaacct aataaattat ccgtgtttgg cgaataactc atgatgttgt
      781 caacgtaatt cgtgcccgaa ctcatattga tctcgatttt gataccaaaa ggattgcgcg
      841 tcatttttgc ttcttctgct cctgtgaatc cgatatctaa gcggaccccg taggcaccca
      901 tcttatggaa aaaagataag tcatcgtaag aaatctccaa ctgttcaaat aacgctggat
      961 tgatatcaac cattacttcc atgcctaggc tattcgcgta atcaactact tctttaaatt
     1021 ctgaaagaac tttttcttta tcatcattga tctgcagcaa actggtaaaa acccgtttat
     1081 acccatactt atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg
     1141 gataaatcga gattcctaat tttcccatat taccatttcc tcctgcatct tttcgttttt
     1201 gtatccactt acattatagc aaacacaaag taaatggtct acaccttttc tttttctttt
     1261 aaaataaatg ataaattcca ataatttaag taggtatttt ccctctattt tgttatcatt
     1321 aaagataaca aaatagtaaa ttggaaagga acagttatga agaaaaataa agttatttta
     1381 agtattagtt tagcagcagc attgctgttt gctgctggtt gttctgcaaa caacacgact
     1441 tcttctagtt cgtctacaga aagttcaaaa agtacatcta ctgcggaaag cacgactgcc
     1501 agtagcgtcg atttgaacgc attggaatta ccacaattga cgaaagatgt cgccgaaaat
     1561 gaagatctgg tcgaaatggt gacgaataaa ggcacaatca aaatcaaatt atttcccgaa
     1621 cacgcaccaa aagcggttga aaactttatg acacatgcaa aagacggtta ctacaacgga
     1681 ctgactttcc atcgcgtcat caaagatttc atgatccaag gtggcgatcc taaaggcgat
     1741 ggcactggag gggaaagcat ttggggagaa ggatttgaaa cagagatcaa caaccacctt
     1801 tataatataa gaggtgcgtt atctatggct cgctcacaag atcctaacag taacggcagc
     1861 cagttcttta tcgttcaaaa tacagatgat atgcatgatg gtcttttaaa agacgactat
     1921 cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc cttcactaga tggccattat
     1981 actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg atgctattgc aaatatcgaa
     2041 acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg ataaaatcaa tatactgcaa
     2101 gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc atagatgaca acgtatgcta
     2161 ggattcaggc gtcttctttt tacataaaaa gcaagtaagc ttccttttgt tttgctatat
     2221 caggttttat agtcaagcat tttggttgac gatgaacgtg aactcgcact cacagacttt
     2281 tttatcatct acatatgctg cagataacgc tgtaccgact gatcccttca ttttggtgat
     2341 ttcaaaatga catttcagca catctcctgg aacaactttt tgacggaatt tggccttatt
     2401 gatcccaccg atataagctc tttttcctag aaactcttct gattttagga taagtatcga
     2461 acctgcttgt gccagtgttt ctagaatcaa cgcacccggc atcactggat tgtcaggaaa
     2521 gtgaccttgg aaaaaatctt cattgattgt tacatttttt gtcgcaacga tcttttttcc
     2581 tggttctaaa gtatctacat aatcaatata acaaatagga taacgattag gaatcaattc
     2641 cattacttca gtagcagata atacgtaaga cataattaaa ctccttcttt caggcaattg
     2701 ccattttcat tactttgact atcaaagtat catattccta aatatttagc aatctttttt
     2761 taatttaaga aaaaatagtt tgacaatcaa aacattttga ctaataatta agaaatcaag
     2821 aagttatttt agctattatt ctctattttt ttacgactat caaattttta tcagaaacaa
     2881 aggagagtca ttatgtcatt tctagaagga aaaaaaatcg tcattatggg cgtcgctaac
     2941 aaaaaaagca ttgcttgggg ttgtgctgaa gcaatgttga aacaaggggc agaaattatc
     3001 tatacttacc aaaatgaacg aatgaaaaaa tcattagtca aattggtcgg cgaagaagca
     3061 ttcactgtag aatgtgatgt ttctagcgac gaaagcatcg aaaaagcaat gaatgccatc
     3121 ggtgaatatg ctggggagat ccacggcctt gtccatgcag ttgcttacgc caacaaagaa
     3181 gaattgtccg gaaatgtgtc tgatatttcc agagacggct atcaattagc tcaagatatc
     3241 agcagctact ctttaattgc tgtatcaaaa tacgcacaac cgtttcttgc accaaacagc
     3301 gggatcgtta gcatgactta tctaggttca gaacgcgcaa tcccgaatta taacatgatg
     3361 ggtattgcga aagcttctct tgaagctgct atccgttatt tggcagctga gttctcgcct
     3421 aaaggaattc gggtcaatgg aatctcagct ggggcaatca aaacattagc tgtcacaggt
     3481 gttaaagact atcaaaaact gatccagtta tctgaatctc gtacgcctga tcagcaaggt
     3541 gtgacgatcg aagaagtcgg taatgcttgt gctttcttaa tcagcccact ttccagcgga
     3601 atcatcggtg atattatcta cgttgacaaa ggtgtccatc tttcataaac ttttgttgca
     3661 ttctttaaag taaaacatat aaaaatcagg aaaaaacttt ttaaagtctc ttcctgattt
     3721 tttcttttta cgtttttttc aactcattac tttgaaatta ttccacgact tcaatccagc
     3781 cttctggtgc ttctatgtct ccaaattgaa tacctgtcaa ttcatcatac aaccgttttg
     3841 ttactggtcc tacttccgtt tcactataaa atacatgaag attgttattg tgataaatcc
     3901 caccgattgg agaaataaca gccgctgtac cacatgcacc agcttctgca aattgatcta
     3961 gatcatcaat gaaaatatct ccttcaattg cttgcagtcc tagtcgttct tcagctaact
     4021 gcaacaagga atatttcgtg atacttggta agatagaggg cgattgcgga gtgatgaatt
     4081 ttccgtcttt tgtaattcca aagaaatttg ctgcacctac ttcttcgatt ttcgtatgtg
     4141 tggctggatc aagataaaca caatctgagt aatccagttc ttttgcttct gttcctggaa
     4201 gtaaactcgc tgcgtaattt ccgccgactt ttgctgcacc agtcccctta ggagcagcac
     4261 gatcgtaatc agatacaata aaattcgttg gttttagtcc acctttgaaa tacgctccta
     4321 ctggcgtaca gaagacgaca aataaatatt ctggagctgg atggacacca atgttttcac
     4381 cgaccccaat cagtaaaggg cgcaagtata atgtagcgcc tgttccatag ggcggcacaa
     4441 aatcttcatt tgcttttacc acttgcttga cagcatcaac gaacatcttt tcagggacca
     4501 ttggcatcag caatcgtcta gcactttgat tcatccgtcg actattttgt tcaggtcgaa
     4561 agagattgac cttgccgtct tttcgacgat aagcttttag accttcaaaa cattgctgac
     4621 cataatgaag cgctggagag ccttcatgaa tcgtcaggta attatcttct gtcaattctc
     4681 cttcttccca ttgtccgtct ttccatttgg caataaaacg ccaaggagtt ttgatataag
     4741 aaaaacctag attgttccaa tcgatttgca tatacttacc accttcgtta ttttctccac
     4801 attttatcat tatccatatt atttgtcatt ttattttcag cttttttcct tcattatagt
     4861 tatttttgct atttcctttt ttgaaaactt cttggtacaa tacagaaaga atcaaatttt
     4921 agaaaagagg attttatgtt ttcattatta gcaactgccg gtacgacaga ctcttctggc
     4981 gagacattga cagatcaagc tgtcagcaaa gtaaatgcgt ttcagcggtt ttgggacggg
     5041 attgattggg acgccatttt ggctgccttt atccaaaaaa gtttatatct gatttttttg
     5101 atcgtccttt tttggattct tcaccgtgtg ggaaaatatc tgattgataa gtcttttaat
     5161 cagtattcta aaaaagccat tctaaacgag agccgcttaa agactttaca tagtctttta
     5221 aatactattt ttcattatac ccttggtttc ttttttatct atgcaatttt atctgctatc
     5281 ggtgtgccga tcggatcact tttggctggt gccggaattg caggtgtggc aatcggtctt
     5341 ggtgcccaag ggttcatgag tgatgttatt accggatttt ttattatcat ggaacaacag
     5401 atggatgttg gggactacat caagctagca aacttaacga ttgaaggaac cgtcgtatct
     5461 gttggtatcc gtacccttca gctgaaagca acggatggta ctgtccattt catcccgaat
     5521 cgaaacatca ccacgatcag caatctctct cgtgcaaata tgcaagtact tttagatatt
     5581 cggattgttc ctgaagaagg atatgacaaa atctatgaga tcattgatcg agtaaatcgt
     5641 acattagctg aaaaatacca agaagagctc caaactgaac cttctatctt tggattagtg
     5701 gatcttggaa acagcaattt tgcgattcgt acggtctgct acgcattgaa cggaaaacaa
     5761 tatgccttaa aagaagagtt cttgagctcc tatgtcaaag aattgacagc ttctggtttc
     5821 acgattccga acagtccaat tgctgtcgga aaataatttg gacaaacgat gcaacgaatc
     5881 tcactaaaa
//
LOCUS       DAFCNS010000113         5565 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFCNS010000113 DAFCNS010000000
VERSION     DAFCNS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.117"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..152)
                     /locus_tag="LNE23_002635"
     CDS             complement(<1..152)
                     /locus_tag="LNE23_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBK6932445.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(245..640)
                     /locus_tag="LNE23_002636"
     CDS             complement(245..640)
                     /locus_tag="LNE23_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932446.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(630..842)
                     /locus_tag="LNE23_002637"
     CDS             complement(630..842)
                     /locus_tag="LNE23_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6932447.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1914..2516
                     /locus_tag="LNE23_002638"
     CDS             1914..2516
                     /locus_tag="LNE23_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932448.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2871..3107
                     /locus_tag="LNE23_002639"
     CDS             2871..3107
                     /locus_tag="LNE23_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HBK6932449.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            3104..3517
                     /locus_tag="LNE23_002640"
     CDS             3104..3517
                     /locus_tag="LNE23_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBK6932450.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            4036..4986
                     /locus_tag="LNE23_002641"
     CDS             4036..4986
                     /locus_tag="LNE23_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HBK6932451.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4983..>5565)
                     /locus_tag="LNE23_002642"
     CDS             complement(4983..>5565)
                     /locus_tag="LNE23_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6932452.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1981 a          806 c          931 g         1847 t
ORIGIN      
        1 acccaacgat aaatcgtagt atgacaaaca tttattccac gatcatataa caattcctga
       61 acttcacgat agcttagatt gtaacgcagg tagtaaccaa cagcgacaat aatgacgtct
      121 tttttgaatt gtttgccttt aaaatgattc attactctgt cctctctgtc ttttttctca
      181 attttacact aaaatagatt ttttggaaaa ctttgcaaca gaacctttgt atgaaccttt
      241 atttctacat ttttttctca agcacactac tgataatgat taaaataatc ggctggattg
      301 caatgatact tccgacaata atagctggaa tgatgtagtc taaacaaata aatataactg
      361 ttgccacccc taacagtatt gtgttaaaca tatttgttat atttcccgct ttttctttga
      421 ttatgatatt ccgttcgtcc ataacattga ttttgtagtc tgcatcattt ttaatcattt
      481 tatcatgcaa aatttgaaca tgagaaacgc caaaaacggc actgaataaa atagcaagtc
      541 caatatctac cattttagga aaatctgtaa ccaaaattgt cacaatcaaa aacaacgaaa
      601 caatataacc gatataccaa atctgaccct tatttttcat cttcactttc ctcctcatag
      661 ataaaaattt cttcaatgct catttgaaag taccttgcaa ttttaaatgc caatataata
      721 gagggattat aacgtccatt ttctaaagaa cctattgttt gacgagatac ttcaagagca
      781 gaagctaaat cttcttgttt aattcctctt tgttttcgta attcttccaa acgatttttc
      841 acaacatgct cctttctaaa aaatggaaag tttactttcc tttcgtgctt ttataatact
      901 cctgcgagca caaaatgtca aggaagcttt ccatataaat tttgatttta atgttttagt
      961 tactgttttt atgacacatg aacacactta ttcttatttc ctcttatctg ctatcaacag
     1021 aaaagaaaaa gccctgtcaa gagccgatta gatcaagcca gtcgagaaac gatgttagac
     1081 caaattgatt ttgggtaatg aatgtttaga acttagtagt catccaaaaa cgactacttt
     1141 ttttgttgaa tcgtccaagg acgattcgat gcttttgact tgtaagaaca ggattgaatg
     1201 aaaccatact gttcgcatat ttttagtgga tgaacaaaca aaatacgaga gattttttgt
     1261 tcgttcatcc atggttttag aaaaaagagg gacgattttg gaagaagaaa atcgtctctt
     1321 ttttttcttc tttttgtatg acaaaaagaa agatcttttg cccattttat ttttataaaa
     1381 tgggtaggtg gcgttcgcgt aaagcaaatc gatacaatcc aatggttttg gcgaatcaga
     1441 tttaccattc taggcttgtt taatacagac atcgattgtt attttgagaa tttatatcct
     1501 tattggcaat gaaatggata ttctccatac caatagaaat cttcaagata attttcggaa
     1561 atatataaga gttaatcaat tatgatcata tagtaggtaa aaattaggac agccttcaat
     1621 tatatgggaa accaatataa tcaagtaatt attcgggtaa cacaaatact agagtactca
     1681 tttcttataa ctgttttaca cctattaggg atcagtatta ttaatagatc tagataaatt
     1741 aatacgtaat tatataataa tttatgtttg tgccattaaa caatcttaac agatatttca
     1801 ttttatttat gtaaaacaac acgaataatt attgcttcgt aaagttatat gagtataagt
     1861 taatttactg gaggtagtgc aatgcttttt tctgaaagat ttggttttaa aaaatggagg
     1921 atatcatact gagagataga ttgacagacc ctcttaaaaa tagtttagca aatgtgattt
     1981 atgatatatt acgagaggct gataaagttt atagtaatta taatggaaaa gtctcatggg
     2041 taatatattc ttttttaatt aaagagtttt ttaaagaaca gataaataat agctatgagt
     2101 tgggttatca ttttactgtt tattatgaag atattatcaa gaggttagaa gacttggagt
     2161 ggtatgctta ttatgattta ttagaggttt ttgcaagtgc taaaatcttg gataaaaaga
     2221 aaagagagga agtaaataaa attctggagt cggaaaattc tggttacaga ttaagcgaaa
     2281 gcaatgaatt tattccaatc acagatgata tttctattaa gtctataaat gctgtatctg
     2341 aatcaccatt taattacgca aaaaatcata tccataaatc tcttacatat ataagcgaga
     2401 aggaaaatca agattataat actgtagtga gagaagcaat tagtgctgtc gaaagttgtc
     2461 ttatcgaatt ttcaggatta ccagctaata aaaaaatact ttgggaactg ctataaaagc
     2521 aattaaagac aaatcgaata acaatgtcga tcttacattt ttaaaaccat ttgagacaat
     2581 gtatggtttg gcgagtaata atggaatacg acatgcagaa aatgaaaaaa caattgtctc
     2641 agatttacct gatgcagttt tggtgttaac aacatgttca gccgtaatta attatttggg
     2701 agtaaaaata gcatatgact ccaataaatc atgaaaagtt aaaattcatc caagagctac
     2761 attataacta ttaatttcca actctttaaa agtttagtaa ttgaaggaag taataagggt
     2821 gattgaacaa tacggatata cggtgtatac ttatcgtagg agttgatgat atgaatatta
     2881 aaaaacgcaa aatcagagaa gtaggtaatt caatagttgt aactttgtca aaagagagtt
     2941 tattgcaaaa aggtttgaaa cctggagata ctatttttat tgatgaagat aaaatgatgg
     3001 aagctattgt aaaagaggaa agcaagatag atcttgaaat tgatatgtat gttaaccaag
     3061 ctttatctga gtatgatgca gctttcaagg aattagttga aaaatgatct atctaagtga
     3121 aaaagatatt gtaaaattga atgttattca aataaagaaa tattcaccgc gagaaacaat
     3181 cggagtcatt gacaaaaatg cgctgagtat ggctgtaaat caacctaaac agcatatttt
     3241 tggaaaagat ctgtatcctg ataaatttag caaagcagct gttctgctaa taaatctgat
     3301 caaaaagcat ccatttcata atgctaacaa acgtacagca tttttagccg cgtatgtttt
     3361 tctcaagcta aatggctatt cactaaaaat gaaaaatcag gaagtagtag aatttgtagt
     3421 tgaaattgct acgtatcagg gtgattttga tgacttaaaa gaggcaacaa cagatatact
     3481 aaaaaagaat tcaacaaaga ccgattcaaa aaagtagaag caaactaggg attcagttaa
     3541 atctgaagat ttagtttgtt ctttatttta gtttacataa gatacattat gcgaagttca
     3601 ttattttaaa agcttaaata ataaactttt ttataatacc aatttattta tgtaaaccaa
     3661 tgaccttaat cgcttaagca agcatgttat gtaatactct actctctcca aaactaatga
     3721 ctatctattt tcaaagaagc tagaatgatt atctgatcaa atgataattt atatttaatt
     3781 cctggtctac tgctcttctt ttaatcaatt aatgtctaac gtactgtaca cgataaagta
     3841 ggaagactta ttttcaactt cggattcatt tttttaattc ggtgaaaatc actaaattaa
     3901 gagtttatcc aagttcattt tagctgagaa tacgcttgaa aataattttg atgaattttc
     3961 ctgaattaaa atttattaac ttgttgagtg atataattgt aatgtattac caaatagtta
     4021 gcacttagat tagtgatgga aaggaatagt gagaacaaga tgactggtag aaatcccaaa
     4081 gtatttatat catattcatg ggagaataag gaaaataaag aatgggctag agaactagca
     4141 gacaaattac ttgaaaacgg tattgatgca tatatagatc agtatgattt aagtttaggt
     4201 gatagattac ctcagtttat ggagcagcaa atatccgatt cagattatgt tctaattata
     4261 tgtacaccag agtataaaaa aaaggcaaat aatcgtacaa gtggtgttgg gtatgaaggt
     4321 catattatct cagcagaatt gctttctgga aaaaatgaac gtaaatttat cccaataatt
     4381 aaagaaggac gtgcaagcga agtacttcct acttttcttg ctggaaagct tggaattgat
     4441 ttatcagata gccagcatta tgatgaaaac ttagatgatt taattacaac tatatttggt
     4501 gttaagagaa aaccagcatt aggtgatata cctaggcata ttaaaggcag tcctttacta
     4561 cctatagaac ataatgaaaa taaagatatt gaaatcttag gaattatcac tgataaagta
     4621 actgtaccta aaatggatgg ctcaaggggt tctgcattat ataaaattcc atttagatta
     4681 aatagagtac caagtcagct ttggaaagag ttgtttgtta gaaattggaa ttcaccacca
     4741 cgatatacaa caatgcatag accaggaatt gcttctgttg tagacgatga aattttactt
     4801 aatggaacaa ctattgagga agtccgggaa tatcatcggg aaactttaaa actttgcatt
     4861 gaaaaaagta atactcaaga aaaaaaaata ttgctagagg aggaacaaaa aaaagagaga
     4921 gagaatgagc gagtcagatc acataaagaa agtgttcaga aattagcaga tgaattagag
     4981 ttctagttga tagtcagttc tttcttaatt cgatacacag tttgtctagt tacattatag
     5041 tctgttgcta tttttgaaat agcaacacca ttctcctggt cctgaacgat gcttctataa
     5101 acaaggcgac gttgaggatc ttttgtgttc gcactgtata acttaggacg tcctttatag
     5161 acgccgtttg ctttagcaat ctttatccct tgtgcctggc gtcgttttga ttcagtacgc
     5221 tcttgttcag caatcatggc caagatttga atgattaggt ctttgataaa tttatccagg
     5281 agaggatttc cgattgcttc agccatgatc ggtaaattag tgataataag tcgcacattt
     5341 ttcttcttta gataatttac tgaatcgata atctcgtcat aattacgtcc aagtcgatcg
     5401 atagcctcta caataaaaat atcttggtct ctaacaaaat ctagaacttc ctgaaaaatc
     5461 ggtcgatgag taatagtcgc tccagattgt ttctcgatga aaattttttc agcaccaaat
     5521 tttctcattt cctcgatttg acgttgttca ttttgatcga tcgat
//
LOCUS       DAFCNS010000114         5466 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFCNS010000114 DAFCNS010000000
VERSION     DAFCNS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.118"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            118..1107
                     /locus_tag="LNE23_002643"
     CDS             118..1107
                     /locus_tag="LNE23_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6932453.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="LNE23_002644"
     CDS             1089..1550
                     /locus_tag="LNE23_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK6932454.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="LNE23_002645"
     CDS             1600..2064
                     /locus_tag="LNE23_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6932455.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="LNE23_002646"
     CDS             2078..2827
                     /locus_tag="LNE23_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6932456.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="LNE23_002647"
     CDS             2827..3657
                     /locus_tag="LNE23_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6932457.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="LNE23_002648"
     CDS             3657..4304
                     /locus_tag="LNE23_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6932458.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="LNE23_002649"
     CDS             complement(4402..5178)
                     /locus_tag="LNE23_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6932459.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5260..5403
                     /locus_tag="LNE23_002650"
                     /pseudo
     CDS             5260..5403
                     /locus_tag="LNE23_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1872 a          739 c          973 g         1882 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtattaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtagat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaataa aagattaaga tataaatata aatgaactag tctttgacta accaatgccc
     5281 gcttttgaaa gaaaacaaat tcattctgtt ttaagtaaaa atccttatgt taccactcat
     5341 tctgaagggg atgagcctta tcgctacttg gttgtggaac caagcgataa gacaatttta
     5401 taaattcaat attaattaaa ataaaaacaa acaaagtaga ttattttact ttgtttgttt
     5461 ttttat
//
LOCUS       DAFCNS010000115         5268 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFCNS010000115 DAFCNS010000000
VERSION     DAFCNS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.119"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(30..434)
                     /locus_tag="LNE23_002651"
     CDS             complement(30..434)
                     /locus_tag="LNE23_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932460.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            complement(427..828)
                     /locus_tag="LNE23_002652"
     CDS             complement(427..828)
                     /locus_tag="LNE23_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932461.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(818..1171)
                     /locus_tag="LNE23_002653"
     CDS             complement(818..1171)
                     /locus_tag="LNE23_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK6932462.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(1161..1472)
                     /locus_tag="LNE23_002654"
     CDS             complement(1161..1472)
                     /locus_tag="LNE23_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932463.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(1469..2344)
                     /locus_tag="LNE23_002655"
     CDS             complement(1469..2344)
                     /locus_tag="LNE23_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932464.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            complement(2354..3514)
                     /locus_tag="LNE23_002656"
     CDS             complement(2354..3514)
                     /locus_tag="LNE23_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK6932465.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(3514..4200)
                     /locus_tag="LNE23_002657"
     CDS             complement(3514..4200)
                     /locus_tag="LNE23_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBK6932466.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(4163..>5268)
                     /locus_tag="LNE23_002658"
     CDS             complement(4163..>5268)
                     /locus_tag="LNE23_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK6932467.1"
                     /translation="LQVDFVYRKFAIETCIDLIANAMSKAEFKSYEDGKNKKNDLYYR
                     LNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSPSNNSSEIFIADSWDVTEYALKDDVF
                     SQVQINNIVLDREFLESDVIYIKYADQQIRQLVDAYYQAYGKLISSAMNVYKRSNARR
                     YVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEADNAGAVFQLQNEYTLEDFSGNFQSN
                     SRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGLSDQVDAFLMFEIIPIAELIQDAFNA
                     SLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAIDVGIRNGVFTINEGRERVGNDRSDK
                     AMADEIFITKNNQQVSKGGEANDDNENISSSKE"
BASE COUNT         1505 a         1089 c          779 g         1895 t
ORIGIN      
        1 agtttttgtt ttagccattg ttgttccccc tatttattta atttatcgaa ttgacgaatc
       61 agaagttctg taattggatc aagtgcaaga cctaatcctc ttctcataaa ttcgtccggc
      121 tgatttcttt tagaagtacc tatccccaaa tcaggatatt ttaaatactc aaattttctt
      181 gtaggtctaa tgatgaaacc caaattaatg tattgagtct taagtggacg actatttttt
      241 gcgtgttggt gccctcttct taaatctgct tcagaaacag gaattttttc tgtaatccta
      301 tccactgcaa tagccgaacc ttttgatttc aatgcttcgt taatcagtcg ttcgctctcg
      361 cttgaatagc gttccatccg cacaagaagt tcatcatgtc catttatttt tagctcccaa
      421 ctatttttag ccatgacaat cactcttcaa taatcgtcta aacgtaaata ccaattgatc
      481 gatatagcga tcttggttct ctagttttaa atgattggga tccattctct gaaaacgaat
      541 cgaacgattt tgaatcaatg aaataatatc tagtgagtct cctgttaaat cttctctatt
      601 ttctgaatag aaagttagat atagattttg acccacgcta tattttggct cagtgatcat
      661 ttctatttct cctgtttcga gaatgaagta attaaaatca tcaggtagct catcctcgcc
      721 tacggagtct tgaaagagtt tgaggccaaa atgctcttct aaggaagttt tgatagcaga
      781 aatttgctta tttaaacgtt ctttttcttt agaattatca atcaccatat tcacccacac
      841 tttcaagata aaaatagata taaaaattat cgtaatcggc atagataacg ttgtaacgca
      901 tactatcgat tacgataaaa tattgatctt tattaaattt cttggcgatt ggatgaaatg
      961 gagtctttac tttcttagtt aatttcgatc ccatcgcatc catagctgtt atatcactat
     1021 ctctcatgga aagatttcta aattttaaag aagtgatttc tgtatcttct acaccaatct
     1081 tttttcctag ttcatttctt ttggtagttt gcgtcaaaat ctttaaccaa ccatcgttga
     1141 atgtttcttc gagtctacga ttattcgcca ttcacatcac ctgcaatata ttcttgtagc
     1201 gcataatgtt gaatgaaacc taataactca ctagcgaaat tttgttcaaa ctcatctaaa
     1261 gcacgattcc agtcgtatct acatctttcg attagcaatc cgtattctaa gctttcagga
     1321 gaaaaagaaa gtgttgtact cactttactt tgaagataaa cagcattttt agctatcatc
     1381 tttttaattg actcatcttc ttcgttccag gtaacgtaaa tattatcctt cacagctatt
     1441 agcaattctt cagtcacttg ttcaggcgtc atctaaccac cgccttaatt gctttaacat
     1501 atgcgtaaga gcattttttc ttgtttacaa atgataaatc ttcatcaaaa ggcgtagaag
     1561 tcacgtatct ccctttgaaa aataaatctt catcgtttgt tgttactcca gcattgtgta
     1621 agatttttac ttctttaact ttttctattg gatcagtagc aaaacaaaag tctaattcct
     1681 cgtgaacttt aggaccaata ttgaaataca tcatgttcca aagctgtgcc cacatctcgg
     1741 ctgtccagat ttgtatattt gttttttgcc ctctaaggta gcgatatagc cgattagaat
     1801 ccagataaac ctttttccaa taattcgctt taggacggtt aataacccac tgtgcgcctc
     1861 ctgaattagt gtttatagtt tccaaagatt ctactgtaac atttacaatg tttgccatat
     1921 cttttagaat attttctccg ttttcacagc ttctaatata atcaagactt agataactac
     1981 agcagtcgct acaataccaa acatcatctt tagaaggcaa tttgcgcaaa ttaattcttt
     2041 tattgaaaat gacatccgaa tcgatataga aatatcggtc gtcctcacgc gaatgatctt
     2101 cttctaaata tttccaccat aaatatggtt taatcgaagg aatatactct ttgtcgtccc
     2161 gcagatcatc gtacacatga acttcaacac catattcctt ctcaaaaaaa ataggaatct
     2221 gatcatcgtg tctgctgaaa agcaatacga tatctttgat tcctagtttc ttcagattag
     2281 ttaaacaaac ttcaagctcc catttaaacc gattgattgc cggctgacaa agaatatact
     2341 tcattctgat cacctacgct tgtgttgtag ttgttgtggt tgttggtttt gtagttgtag
     2401 tagtagttcc caaagcgcta atatctaata caatgaaact atcgttacgt ttaggttgac
     2461 cgtttgcata ttgtttagct agataaatgc gttcgtcttc aacaaaatgg tattcatctg
     2521 aagcttcaat ttttagtgta gatcctacac ccatgaagta atctgaggct accccaataa
     2581 ctgcttttcc ttctggcaca gccgttgact gcaaatctga aactggtact ggcaatactt
     2641 gtacgtattc tccattagca gttagtacag tcttagctgg gaatacttta gaccagtaat
     2701 cagttggatt cacaattagg accacatcag aaggattcac attacgataa atcggatcat
     2761 tcacaccttc gatattgaat tttgatagtc gcgccatcaa accgcccata gttacagcat
     2821 ctaaagctgt aataggttct gctttttttt cagcatattc tccgctagtt tgtttgctca
     2881 tgtcacgcat cattccgact ggcatatctt taccagtacc atcaacaatt gcttgttcta
     2941 atgcaattct caatgattct actaaaacag tacggacata acgatctaac catactggac
     3001 ctaaatcaag cattgcctta catacaggaa tataacctga tagcttgaac tgcttcatgt
     3061 taattacatc aaagccatta tctaaaactt ttttaacagc ttcgcaaagt ttaccccacc
     3121 atgctggatt gactccacgt gacacaatcc attctgttac accagttgtg ttaacaaaag
     3181 taattttttg caatagtgga tgagattgtt ctaaatcttc aaatacacgt tcaaatacag
     3241 tagctggcac taattcttcg acccctgcaa aaccttcgtt tttcactact tcgttataga
     3301 attttgtttc ttgtgtagtt aatacacgct gaccacggtt cattaatact aattgatctt
     3361 gattttttgc tgttgcttct tctaaaattt tatcctgaat ttccttagat aagcttacca
     3421 tagctgcgct aaaagattct tcgttaccat ctttaaaagc tttcatcaat tggtcgcttg
     3481 cagctgttac accttttaaa tttttaactg tcattatttt acatctcctt gtccaaatgt
     3541 tttatttaat gctgctgtaa atgcagcaat tttttctgct cttttttctt taacgtcatt
     3601 caaaatttct tcaacgcttt gttctttttt agcttcagta cctgagctat tttctgcatc
     3661 gataatttca tcgaccaatc cataactcaa agctgtttct gcatccataa acgattcttt
     3721 ttcaagaagt tcttgcaatg cttcatctgt gccattgaat cgtgttttat atgaagcctt
     3781 taccgattta tcaattgatt ccagttggtc agcaatcgta cggaagtcat cgacatttcc
     3841 ttctccgtat gtggaagcgc ggtgaatcat caattgtgca ttgttgtaga tttttatagt
     3901 atcgccagcc attgcgataa ttgaagcagc actagcggct aagccgttaa tcacaacgtt
     3961 aacttttgct ttatttgact taagtaagtt cccaatagca atcccttgaa atacgtctcc
     4021 accgtttgaa ttaattacta cttcaatttc ttcttgatca cctagactat ccaaaatatt
     4081 tttgattccc ttgtcagtat tcccttcaaa gaaccaacta gaaccaataa atccctgaat
     4141 aaaaatttgc ggtactgcgc cttcattctt tactgctaga aatgttttca ttgtcgtcat
     4201 tcgcctcacc tcctttcgat acttgttgat tgtttttagt tataaatatt tcatctgcca
     4261 tcgccttatc agagcgatca tttccaacgc gttctcttcc ttcgttgatt gtaaatactc
     4321 catttctaat gcctacatca atagcgtcaa ccaaatcttt gaagctagta atcttgatca
     4381 tagttgtatc cacacgtaca aaattccctg acaagtattc ttctacttca tagagactag
     4441 cgttaaacgc atcctgaata agttcagcaa tcggtatgat ttcgaacatt aaaaaagcgt
     4501 ccacttgatc cgataaccca ctcatgtctc cctttagtag gtttttcgga acgtgaaacg
     4561 ctgctgctgt catctcaaag atgtcgtcta ttaagttttt tatatctctt gaattgcttt
     4621 ggaagtttcc gctgaaatct tctaatgtgt actcattttg taattgaaat accgcacctg
     4681 cattatcagc ttccataaaa gccttaaatt gtgatgtcat cattttattg atttgatctt
     4741 gtgttgtatt gtcttgcggt cggaataaat tccctttcag tacgtatcta cgagcgttag
     4801 agcgcttgta aacattcatg gcactagaaa tgagtttccc atacgcttga taatacgcat
     4861 cgactagttg cctaatttgt tgatctgcgt attttatata gataacatca ctttctagaa
     4921 attctctatc aaggactatg ttgttaattt gcacttgaga aaacacatca tctttcaatg
     4981 catattctgt gacatcccaa ctatccgcaa taaatatttc gctagaatta ttagacggag
     5041 aaacgatcaa tacttcattg tagaatatta atctcctgat cagttttttt ctaaattctg
     5101 ttgcattatt tttcttatta ggagctacat tcagcctata gtaaagatca ttctttttat
     5161 tttttccatc ttcatatgac ttgaattccg ctttactcat cgcatttgca atcaaatcaa
     5221 tacaagtttc aatcgcaaat tttcgataca caaaatcaac ttgcaatt
//
LOCUS       DAFCNS010000116         5068 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFCNS010000116 DAFCNS010000000
VERSION     DAFCNS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.120"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            416..1570
                     /locus_tag="LNE23_002659"
     CDS             416..1570
                     /locus_tag="LNE23_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBK6932468.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1564..1986
                     /locus_tag="LNE23_002660"
     CDS             1564..1986
                     /locus_tag="LNE23_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932469.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3156..4124
                     /locus_tag="LNE23_002661"
     CDS             3156..4124
                     /locus_tag="LNE23_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6932470.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            4141..4665
                     /locus_tag="LNE23_002662"
     CDS             4141..4665
                     /locus_tag="LNE23_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK6932471.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
BASE COUNT         1903 a          842 c          839 g         1484 t
ORIGIN      
        1 aagactgctc tcacgagcag cccaaaatga gattatccta ttcatcaaca taaaaatatt
       61 acaaagtgaa gaactagaga aagacaaaaa aagcatatca aatcctctca aaaataacaa
      121 gtctttaaaa aatgacagat aaaaatgcac aaagaactaa aaaagagaaa tcgcgtaaaa
      181 tcaagcatta gaagctagta actagctaga ccaattaaaa atcacaaagt gagcgtctct
      241 atccattggt agaggggcta cttggtctag tcgacattat gttaactagc tcgataaata
      301 acgattatgt tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt
      361 gtatatttta attatataat tataggtgta taccacttaa atttaacaca aagacgtgat
      421 tattttgaaa cccaaaaaga aaatttatct aggtctcctc ttttccttga tgattggaat
      481 ttttagtata ggaattacaa atacctatgc ttcagaaaat tctaatttca atatcaactc
      541 ccaaccaatt gaagaacaag caattaattt tgcgaaaact gattttgaac actccataac
      601 ttcaattctt ttaatggaaa ataacaccga aactgatatt aactatactc ttggttcgcc
      661 atttaaaatg ctcgatggag aaaatacggc aaatagttct attttctttc cagtattttc
      721 agacgctgat aatacgatta aatatattta taccgtcaaa aaaaatacaa atggagaata
      781 ttcaggaagt atctcacaat ttttagcaaa agaacttcaa aatctagtag atagtaatat
      841 aacagataca accttatttt ttcaagataa tagcatttat tattccgata atggacaaat
      901 taaactactt tggaaatcac ctttaagtac agaaaaaaat ggtgtttctt cctttacaga
      961 aaaaaatgat cttatatccg taaatataaa aaataaaatt aaaaatttct ctaaaaaaca
     1021 aaattttcct gcggttaaac gtgtaaaaag tgtctcctcc ggagataact ttattatgat
     1081 taattggcgt atagcagaaa cccaaacaag ttttccgtgg tgtgctgctt acgtagaagc
     1141 tgctatctta tgtaacaaaa cagatgtacg tgctacttca gctagagcca ttatgcatta
     1201 tacctatcct aatttaaaca acagtcaaat tgaatcaaaa agtcttaatc aagatcaaat
     1261 aataaaatat gcaaattact gtggtgttta tcctaaaaaa gtagcacgaa tactaaacct
     1321 ttcagaggtc caaacacaac taagaaaagg aaatgccatt tatatgggaa ctgctggtta
     1381 tggaaattat agtaacagcc gtcatgcact tgcattattt gggtgggtca atcaaggtgg
     1441 ttatcaaact tactatgtgt ggaatccttg gtataactac ccaactgtaa taagtgcaaa
     1501 tgcaggacca gttacaattc ctgtacctgg aggtggtttt acttgggaca ataccattac
     1561 aaattggtaa ataaacccat tgctttagtt atgggtctag tgttagttct tttcttaact
     1621 agttgttcaa aaaaacaaga aatacaaaag actgatctga ttccttttct aggaacttgg
     1681 caaactaaag atcattcaga acgtctagta atcactaatt taaatacttc aaaaaatata
     1741 gaacaatata ccatcttagt aaaagagaac aatcataaaa ttcaaatcat tgataaaaat
     1801 ttaaatataa atgaacaagt tttcatttac aaagcaacaa ataatttact aacactgcgc
     1861 tctaataaaa aacaattaga ttacactttt agtttggatt ctccaaaaaa gctcgtattt
     1921 attttgacaa cgaatgatgg gacagaaggg acagctaaac caattgaatt ttttaaagct
     1981 gattgatgaa tagttgtttg atattacaaa acaataattt caccgaaggt gaaacaagcg
     2041 aaactttaca aaaagtggct agcatttgct agcctttttt gtattgtgga gcgtgggttt
     2101 tgagggggat tcaaggggga gcttgcgccc ctttgacaag cggttatttg caccaaatac
     2161 ggaggtattt ggctagcaaa tatagagctt gccaaacact ttagtgagtt ttgtagaaag
     2221 tacctttaaa ggtactttca cattacgaac gatacgtccg taggacgtac tgaaagggtg
     2281 ggatttatga gcgaactaat gcaaaatttg gctagcaaaa aaagttaaaa aatatatgct
     2341 aataatcgag cgaaatgaac actttttcaa gtgagcgaat ccgccatgac aaagctgaag
     2401 cagtttattt cagctttgtc atggcgtgtg cctagacttt taaaaaagtc ggcacacgag
     2461 caaatatctt tatcaagctc taatttgcgt tttaagacgt ttgaaacgtc taagtatata
     2521 aatatataag agtaggttta agagtgctta aatcgaattt tagagggcat tcagacaact
     2581 ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc tttaagaaca agataaggga
     2641 tttatactaa gactgtctta aaacgtctca aacgaaggct catagcttct caaagagttc
     2701 cttatgaaca tttttagcaa aggagccgga agcgactgcg cagtataatg tgagagcaga
     2761 gcgatcacac ctttttaagc gactgtaggg agtaagattc ccagttgaga aaaatgcgcg
     2821 aatctgtttg gagaaaaaag aagtggtttt ttaggcggga aattttgtcc ccctctttta
     2881 taatctcttt ttaaacctct ttttaaacct ctttaccgtc ggctatatcc ttactctccc
     2941 aagggaatga cgcttttatc tacatcgttt tgtctgttta tctacatcgt tttgtctgtt
     3001 tatctacatc gttttgtctg tttatctaca tcgttttgtc tgtttgtaca ttgaattagt
     3061 acatcgacga tgttaatata agatgtagat aaacaggcat tttgtatttc ataacgtgta
     3121 tctcttgtgc gagtaagaaa ggagctattt taaatatgtc taatgaaatc gtaaagtatc
     3181 atcatgaatt aaatacaatt ccgttgcgaa aatttacacc agtcgaaatg aatttgttct
     3241 tttctatcgt atcaagaatg cgtgatgtag gtgataaaaa agtacaattt acattcgagc
     3301 aattaaaaga tttaagcaat tataaagcaa ctgcaaatgt tcgattcata gatgatttag
     3361 aaacaacgta tgacaaatta atggatttac gttttggtag aagaagtgct gacggtttgc
     3421 aacgtgaacg ttttgtttta tttaatcaat ttaaaatcga cggaaaatct gatattcctt
     3481 ttgctgaaat acaagttcat gaaaaagcct tacccttact taataatctt gaagaatggg
     3541 tgcgctattc tctccaacaa ttcaatgaat tagaaagcag ttattcaaaa acaatgtttc
     3601 gattactaaa acaatacaga accaaaggtt tcgcttattt ttcaaaagaa gactttcatg
     3661 aattacttga tattcctaaa agttacaacc aaggtacgat tgatcaacgt gtgttcaatc
     3721 caatacgaca agaactaacc gcaatcttta aaggtttaac aatcaaaaag aaatacggaa
     3781 aaggacgggg aaagcctgtc attggctatc aatttacatt taaacctgag ataaaaaaat
     3841 cagacgactt taacaaacat gccccaacaa aagaagaaca aaacaaagca ttagaactag
     3901 ctaaccaaca agttcaatct gaaaaagcac ctacttcatt acaagaaaaa atcaaagaac
     3961 aaaagcgaaa attggaactt ttacaacagt tagaaaagtt agaaagagaa caaaataccc
     4021 aaaataaaga aaataatgca caaaaatcag agataaattc cgatattcct tcagaactaa
     4081 aaaaagactt attagacaat cttcaaaact tattcaaaga ttgaggggtt tttagatttt
     4141 atgacagaaa aaacaatcaa agaacttgct gaagaattag gagttagtaa aacagcaatt
     4201 aacaaaaaag tttctgacga aaatcgaaaa ctttggtttt cgaaaatcgg aaacaagttt
     4261 gtgattaatg aaaccggaca aaaggctata aaatcaatgt tttttaacga aaacgaaaac
     4321 tcaaatcaac aactagttgg cgaaaaaacg aaaaccgaaa accacaaaaa cgccaaccaa
     4381 gttcccgaat tagaagtcct ttctctccta gaacaacagt taaatcaaaa agatatgcaa
     4441 ctacaagaaa aagacgagca aataaaacaa atgcaaaaat tactcgacca ccaacaacaa
     4501 ctcaccctgc aagccaacca acaaatcgag aaactgcagg aacaactaca gttgacttat
     4561 acggaagaat cttcggaaaa taaatcaacg actctttctg aagaaacaga atcgatagaa
     4621 aaacaatcaa aggcggagaa aaaatggtgg caattctggc aataaaacca gcaatttaaa
     4681 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
     4741 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
     4801 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
     4861 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
     4921 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
     4981 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
     5041 atggacattt tagttcgaaa tatcaatc
//
LOCUS       DAFCNS010000117         5029 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFCNS010000117 DAFCNS010000000
VERSION     DAFCNS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.121"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="LNE23_002663"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="LNE23_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBK6932472.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="LNE23_002664"
     CDS             complement(2066..3064)
                     /locus_tag="LNE23_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBK6932473.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="LNE23_002665"
     CDS             complement(3290..3943)
                     /locus_tag="LNE23_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBK6932474.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="LNE23_002666"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="LNE23_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK6932475.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1493 a         1035 c          765 g         1736 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttca aatctaattc aaattttcta aacatatgaa acagcgtata
     4921 aaactgtact attattttat ctcaaaagct aaatgaaacc gatttcttaa gattttttat
     4981 gttttttatt tctttaatag taatgttttc tgtcatttta aataatact
//
LOCUS       DAFCNS010000118         5025 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFCNS010000118 DAFCNS010000000
VERSION     DAFCNS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.122"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            283..522
                     /locus_tag="LNE23_002667"
     CDS             283..522
                     /locus_tag="LNE23_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932476.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="LNE23_002668"
     CDS             complement(884..1144)
                     /locus_tag="LNE23_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932477.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="LNE23_002669"
     CDS             complement(1329..1826)
                     /locus_tag="LNE23_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBK6932478.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="LNE23_002670"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="LNE23_002670"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBK6932479.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="LNE23_002671"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="LNE23_002671"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBK6932480.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5015)
                     /locus_tag="LNE23_002672"
     CDS             complement(4548..5015)
                     /locus_tag="LNE23_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932481.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1529 a         1043 c          771 g         1682 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaa
//
LOCUS       DAFCNS010000119         4871 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFCNS010000119 DAFCNS010000000
VERSION     DAFCNS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.123"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..746
                     /gene="vanR"
                     /locus_tag="LNE23_002673"
     CDS             51..746
                     /gene="vanR"
                     /locus_tag="LNE23_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK6932482.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="LNE23_002674"
     CDS             724..1878
                     /locus_tag="LNE23_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBK6932483.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="LNE23_002675"
     CDS             2093..3061
                     /locus_tag="LNE23_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK6932484.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="LNE23_002676"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="LNE23_002676"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK6932485.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="LNE23_002677"
     CDS             4091..4699
                     /locus_tag="LNE23_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBK6932486.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(4779..>4871)
                     /locus_tag="LNE23_002678"
     CDS             complement(4779..>4871)
                     /locus_tag="LNE23_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBK6932487.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         1501 a          936 c         1135 g         1299 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaaatc ccttacggta tctatgattt aagctgggat tcccaataat
     4801 accttgattt cagtacagac cgaaaacccg aagagagtgc cttcttttcg ggttttctta
     4861 tataatcctc g
//
LOCUS       DAFCNS010000120         4735 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFCNS010000120 DAFCNS010000000
VERSION     DAFCNS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.126"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(16..1101)
                     /gene="xerS"
                     /locus_tag="LNE23_002679"
     CDS             complement(16..1101)
                     /gene="xerS"
                     /locus_tag="LNE23_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBK6932488.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3177..3695)
                     /locus_tag="LNE23_002680"
     CDS             complement(3177..3695)
                     /locus_tag="LNE23_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBK6932489.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1555 a          777 c          718 g         1685 t
ORIGIN      
        1 tattttttac tacatttaca aagcgttcaa agcatcaatt gtagctgcat tgtctatatg
       61 agtatataaa gttgttgtat ttgtacttgt atgtcccaat tgttgggcag ttaataccaa
      121 tgaatttgtc tgttgataga gtctagtagc caaagtatga cgtaatttat gtggactgac
      181 ttggactctg aatccttttg aatattttgc taccatccgt tcgattgcaa taccggatat
      241 acgcttggct tctccttgat aaattgctag aaataaagct ttttcgtttt ctgttacttt
      301 gtatcgttct ttccgaatag ctagataatt agctaaatat tccattgcaa atgctgctat
      361 atttactgcg tctctcttat ttccttttct agttactacc acattattct tttctaaaga
      421 taggtcctct aaattaatat ttacaacctc ggagagtctt aatccagatc ctaaaatcaa
      481 tgcattgatc gccaggtctc tttctacatc tcgttgataa tagatcaaag ctcgtttgct
      541 caaagttttt ttatattcat tggcaatata atctagataa ttttgtgtat cttcgtctag
      601 aaataatttt ttctcgattg cgtttgcacg ggcagctaac gtagttttat ctgtgatagt
      661 tgctacttga tccataggat tattttctaa gtatggtttt cctaaaggat tattggaact
      721 tttcgaaaga tatttaaata gaacctttaa tgctgtgata tttctattga tagtagaagt
      781 agatagacct aagttattat tatctttgag cttagttttt gttgtctctt tttttacatt
      841 ttcctttgtg cgacgtttca aatgagattt gtacaagttt agatcatagg cagttaactg
      901 tcttatcgag gtgattgaag tatcgggaac gatagactca ctcagaagcc attcataaaa
      961 gagcttataa ttttttagat attctaaagt agttcgaggt gattgatctt tatcctcacg
     1021 atcttgaaca aaatctttta tataatcggg catatgaagt atgattgtat ctattttttg
     1081 atatatttct gtatttttca tgttattcat cctttcgata agtaggggag aggagtatct
     1141 ctataattgg actataccaa ttatagagtg ttttgtcaat tggtatactc tgaaatttat
     1201 taatttattt caaaaaagca gataaatagg tatagattta ataatttaaa ataaatggta
     1261 tacatgaata ggaaaggaaa cagaaggagg caatcttaat tttttaaatt gtctaaatta
     1321 tatagaaaga acaatctttc tatataatta gacataagag agactacggt tgatttgata
     1381 ttaacaagcg tatatgaatg tatatacaaa taaaaaaggg gagggagaag ttaaagaaaa
     1441 ttcagagaag tggggcactc agtagtatta acaatttaaa aggaatttct taaagaaaat
     1501 gggttgcatc ctggagatac aattttttta gataaagaaa aaattcaagc aacaattcaa
     1561 aaaaatctta actgctgaga ttgatgtagc aatatctcaa tcatcaacaa agtacgatcc
     1621 tgcctttcaa aagcttattg ataaatgttt attcattata aaaataaaag aaaaatttat
     1681 gtcagctttt ttcatcaaag tggtaatgat aattcgtcta ttaattgtat tttttaggaa
     1741 atatggtata attgttttaa cgaaaaggta attcatgata caccaaaaac cactagctgg
     1801 cgggctagtg gttttctttt acgttctatt cagattaacg ggtaggctta ctgtcttttt
     1861 tggcaataat tttgcaatca agccatttac agatgtaatg cgcggatact tctgctaaga
     1921 ttgcaaaaaa catatcgaaa aggtaattca taatacaacc tcctcccctt gccataatag
     1981 gccggggtag cagacagcat aaatattata ccataactga aaatttcatt ttagtacatt
     2041 tcaatatatt gatgaaccag cacaataaaa catgccatga ctaacagaaa aaaacttatt
     2101 aaaccagata taacttgtaa acatccaact atatagaaaa cgtaaatttt ctatatagtt
     2161 ggattaagat aacgactatt tattttatca tatttttact ataccctata cttttacttg
     2221 gtatatttat gaataatcgc aaatttaccc ctatactact agtttgtaag ttagattaat
     2281 aaaaatacta aagttatttg ttcttttatt aaatacacct acaacttata aattgatggt
     2341 atttagataa cacgaaagaa taaaaataac agctgatagt aaagtttgtt gacgattgct
     2401 actcaatcga aagtgagtta gtgcgtatga acttgcttct gaaaacttat gaaatgaaag
     2461 accttttaca tcttgcagaa atcgttactg ggattaaact aagcaataaa aactaatgtt
     2521 cccctcattc tttgaacttc tactatttga tttatttatt ctgataaatg acgatttcac
     2581 cttcaggatc ttcaaatccc gtatagaatt tatttaagat ctcttcagta tcagtaatag
     2641 gataggcgat aggccacata ccctttgatg gttagaataa actgctcaaa atctgtatcg
     2701 tatgttggct ttagcatatt aacaaaggct atctccaaat tataaaccat aagcgttgaa
     2761 tcgttcttaa caatttcaat tcttcctaac ccaaagtcag aagatataat taccggactt
     2821 gccttaccct tcatgtgggt tgttgagatt ccatatttaa agggaattgt cggtactatc
     2881 ggcggtctga aaaacatccc aaaaataaaa aaatctttcc cctgctagtc tcctaagtcc
     2941 ctttgtagcg gatatggcac cataatctca ttttatcgaa gtgagtcatg gtttatctgt
     3001 tggccaacca aaattgatac aatctaacga tgcaccctct acggcattct aggggagtgc
     3061 acttgcattc acccaataaa aaccggcagt tagcaaagaa cttatgtagt cgtatgttct
     3121 tactgttaac atacaagagg tctcctttcc acaattgctt agcagtaaac gggaatttac
     3181 aaagctaatc tcttccattt tcttatcttt gttctaaaag tcccaaatgg agcaactgta
     3241 tttacatgaa taaatttata tacttcccat acagccgttt tggttgcctc gtctgcccat
     3301 tttcttttgt gtgcagtgaa tagatcttca tcactcattg aatcaatcat catgttaata
     3361 tcatcaatat tacgattgag aatttcctct aattctttca atggtagatg tgcataggta
     3421 tttgtaaacc acttatataa ctctcctaac tgattccatt taaattgatc agaaggcgtt
     3481 ttaacagata atccatttct ttcatcattt tcccatttta gaattaaatt cgtccaacca
     3541 acttgatatg ctaaattttc agcaggtgtt ctatccactt cctcaactct tttatccttc
     3601 aattcttcag gaatagtttc aaattctaaa atatatttag tataagtcct atttatttct
     3661 ttttttaatt cttctttatt tttatatgtt ctcattatat tacctccata aattcctaat
     3721 tgtctccttt aattcatcta taatttcatc cacgatattt taatccgttc tacaatgcat
     3781 atctgatatg cattgactgt ctaactcata tgtgaatgat ttataaaata tcccaacgtc
     3841 tcatctaaag aaatgttgat cgtcagcaag gagctaaaaa atacgaatat atttcttttg
     3901 tagtaaaatc ggactggttt gcatatcatt ttcttcgtaa atatcaaaat catgttaact
     3961 actatcaatt actaataaaa actaccactt taagcgttaa gaactgttaa gtttagttaa
     4021 gtgaggatag ttcacaaaat ccattgttaa acttcacgaa agtggtaaaa ctcaaaatgc
     4081 tttatatagg attacagcat cgctctttct tcgattgcgc gttgggtaaa aaatattatg
     4141 aagtaaaaat tgacgatgac acgttcctta cggctcatca gatgaaacag ctacaaaaac
     4201 gaaatgatca actcgaagaa aatttaattc taaaaaagcg attgccatat tcacgcctcg
     4261 ctcgacgaac tattaaaggt caagatttac ctgaagatca tcctttttag gaaattgaag
     4321 ctttttatgt tgcacaatgc atttataata ctacaatgat gttttcacca gatattcttc
     4381 tagggtgcgg tgtaatgaaa cagatacact tgcaaaagaa aattcaccgc gaatttacta
     4441 aaattatgaa taattatgtt aatcttccga aatttttttc caaaattaag ggatcaagct
     4501 ggtgttatag attgtttaag gttggcaaaa aaataacttc tttttaaaca attatagaaa
     4561 accgatactc tgttaagttg gttgacacgg tgtcgatttt tatgactttc ttattctgaa
     4621 acttcactaa cggacagtac ctaaaaagtc tatagtatgg acgtttacga gtactatttt
     4681 atcaaaaatg ctgctcattt caacttatat gttttagtga actaccccca cttag
//
LOCUS       DAFCNS010000121         4698 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFCNS010000121 DAFCNS010000000
VERSION     DAFCNS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.127"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..142
                     /locus_tag="LNE23_002681"
     CDS             <1..142
                     /locus_tag="LNE23_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HBK6932490.1"
                     /translation="SFEKLKPTHEGRYFRRLNRQHRVVYKVDEDVHVVEIYSARTHYE
                     SQ"
     gene            219..548
                     /locus_tag="LNE23_002682"
     CDS             219..548
                     /locus_tag="LNE23_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6932491.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            538..825
                     /locus_tag="LNE23_002683"
     CDS             538..825
                     /locus_tag="LNE23_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6932492.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKTLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1111..1968
                     /locus_tag="LNE23_002684"
     CDS             1111..1968
                     /locus_tag="LNE23_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6932493.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1969..2517
                     /locus_tag="LNE23_002685"
     CDS             1969..2517
                     /locus_tag="LNE23_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932494.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2777..3505
                     /locus_tag="LNE23_002686"
     CDS             2777..3505
                     /locus_tag="LNE23_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK6932495.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWSKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLRYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            3535..4644
                     /locus_tag="LNE23_002687"
     CDS             3535..4644
                     /locus_tag="LNE23_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBK6932496.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEEAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYSKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAINTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIEHVKKIMSFS"
BASE COUNT         1707 a          622 c          971 g         1398 t
ORIGIN      
        1 atcatttgag aagttaaaac ctacacatga aggtaggtat tttagaagat tgaatagaca
       61 acaccgggtt gtttataagg tagatgaaga tgtacacgtg gttgaaattt attcagcaag
      121 gacgcattac gagtcacagt aattacttgt aaatacatta tctcgtggta taatgtattt
      181 actaagtaat tacatactgt tagaaatgag ggatttaaat gattacaaaa acaagaaaac
      241 aaggcaattc aatcatgttg acagtaccta aagattttaa tgttcctaat ggagtcgaag
      301 ttgaagcaaa gttggtagag aatggtattc tttatgaatt tgttgaacca caaaaagaat
      361 tttatgattt tagcgaagat attttgtctg atataattgc agaaggctac gataaagatg
      421 agattcttgt agaatttaag aatagaaaaa acaaaatgca ttcttccttt agagatattg
      481 ctgaagatac acttactaat tctaaagtaa tgactaaaga ggagttggca acagaaattg
      541 gcttatgaaa tcgttccttc aaaacatgtc attaagtatt taaaaaagct aaaagagaag
      601 acattgaaag aaaaattttt gaatttaatt tatgatgaaa ttgcgattga tccatataag
      661 ggtgaacaaa aaactgggga tttatcaggt atttggtcta gtgggttcaa atatgcgggc
      721 acaacatata gagtagctta cgagattata gaaaacaagg ttattccagt attgttatgt
      781 ggaacgcatg aaaactttta tgaacagttg aaaaaattaa tctagtgatt tgttgtcgtt
      841 cttaagaagg acggcttctt ttttatcgaa gatctgattc atctacatga ctaaagtcgc
      901 gagtattctc tgcttctcga taaattaaat aacgtcatta ctttgaggaa agctataaat
      961 caatatattt cagcttttcc ttatttatgt actacctttt acttattaat tataaggtag
     1021 tacatatata tctttttttt ggtagtaact aaaaataaat atgttataat agtatcaaat
     1081 gaatattgct aaaagaaaag aggtaggaac gtggtagcga agaaatatgt aatcggtaat
     1141 tttaaaggtg gcgtaggtaa aagtacgtgc gcgcaaatgt tagggtttga atcagcagtt
     1201 aatgaaggaa aaagaacctt gataattgac ttggatatgc aaggaaatac ttctgatgtg
     1261 atgaacttaa cgcatatgaa cttcactaac gaagaaggag gcggagaagg tgaaccgatt
     1321 gtttacgaaa atactgtgac tgacgtgttg ataaacaatc ttccacctaa agaggctgtc
     1381 tataaagtaa tcaataacct ctatatactt ccagcagaca tgtcctttga aatgtatgat
     1441 gattggatca aagaccaata taatgattca ttatcccaat tcaaatatat gaaagaaaag
     1501 ctagaaccat tatttgatga gtttgacgtc atctatttag atgtcccacc ttctatttct
     1561 gtatatagta aatccgcgat gtacattgcg gactgggcaa tcgtcgtctt acagacccaa
     1621 gtaaaatcga tgagaaatgc gatgcaatat cttgagtata tggacttttt tgtgaaagaa
     1681 tttgatacaa atttatatgt tgctggtgtt attcctttca tgttggaaag cggtgacgca
     1741 gtagatcagg aaatgtataa acaagcccaa gagatttatg gggaacattt gattaaaaat
     1801 gttgtactca aaaatgcgcg cttaaaacga tacgacggat caggtattac gatggagaag
     1861 acaaagaaag gtctcttaaa acaatgggat cgcaaaagcc acgaattatt tatcagtatt
     1921 ttgaaagaat tggaagaaca cgagaattgg tatgcggagg tggagtaatt ggctgacatc
     1981 aaaggacttg atatttttgg gaacaacaaa ggactcgcca aaataaaaaa agaagcagaa
     2041 gcaaagaaaa agcagcaagc aattacacca tctgaaaata agcaattaga agtgcaccaa
     2101 gttcaagaaa taaaaacttc ctccaatgtt gaaagacaga gtagaggaag aacaaaaaaa
     2161 gttggcgcac caatcagaaa gtttgataag gtttattcca ccaagcatcc tttaaaactg
     2221 tcgactttgt taaatgcgac ttccagagtt ttagtagaaa agtatgagac taatatgaca
     2281 agagatgaat tgcttagaaa agctttagat gagtatatta aacagaatct tacgcaagaa
     2341 gataagcaag atttgtttaa tgacgtggtg aaagaactag acttgttcag agaaaaatat
     2401 ccaattgttc cagaattaga tgatcgtgga gaaataataa gaactaccag tcagataaaa
     2461 caagataccg aagactcact gcgcaaaggg tggggaatga ataataaagt tagataataa
     2521 tcaaagaaga actaagtaac tacctttaaa cggtagttac ttagttcttc tttgatttgc
     2581 gtgaaataga ggggatttat ttttctggtt acgtgattaa aaaatccaaa aaatgagcat
     2641 aggaacggtt atgattttag ggtgtgtttt aatgagatat tgtgatttca ttaacaagtg
     2701 tatgataatc tgttgttgag ttagtaaata agtaatagag ctaactctaa aataaaacta
     2761 ttaggaggag atgtaggtga aaaagtttaa aaagaaaata tatttaggat tggttgttgg
     2821 ggtgagtatg tttgggttca tggctcatac taaacaagta actgcagata ctaaccaaaa
     2881 agaaaggatt atgagccaat tagatgactc tcaaatagag tatgtaccca atgagctaat
     2941 tgtcagttta aaggctacac aaagaatttc aacagagaaa aataaaccgc aaaatttatt
     3001 gggtgtaaaa gttatggtgg aaaaagattt gactcttagg acaaatgaaa gcgattataa
     3061 taagcgagat caagtattat tagtgaaatt gaacgaagaa attagtgtgg aagaggcagt
     3121 aagaacattt aatgcttctg atatggtcaa ttatgcagag ccaaactatg tggttgaact
     3181 agagccgttg aatgatgttg aggagccaaa tacaactgat tctttaggaa atcgatcaat
     3241 tattcttaac gatcctaatt ggagtaaaca agctaattta agagagatta gcatgccatc
     3301 cgcttgggaa gaaaccaagg gttctaagga tgtgaaggta gcggtcattg attcaggaat
     3361 tgattatcaa catgaagatc tttctggaaa tgtagatgaa agtttgagat acgatttttt
     3421 tgacgatgat acagatccta tggatactat gggacatgga cacatattgc cgggattatt
     3481 ggtgctcaaa caaacaacga attaggtgtt gctggaataa attgggatgt tacgatggta
     3541 cctatacgtg tatttggtag tagtggaggt accagtttgg caatcttaac tgaagctatt
     3601 aattatgcat cccaaaaagg gtttccaatt atcaatttta gtataggtag tgttgtttat
     3661 agcagtacag aagaagcaat taaaaactat cctggattat tcgtagcagc agctggaaat
     3721 aatgggatag atatgactga taaacctttt tacccagctt ctttggatat acctaatatt
     3781 attactgtag gaaattctag tggttatgaa cgaagttctg attcaaatta tagtaaaaca
     3841 ggtgttgatt tattcgctag agggtttcgt gtttttagta catttctagg aagtaaatac
     3901 acgtatatgt caggtacctc gatggcaaca cctcatgtca ctggtagtgc tgcattagca
     3961 ttgtcactag atcctgatct atctactgat gaattaaaaa aaatcatttt aaatagtgtt
     4021 gattcatttt atgaactatc tgatttatgt cttacagggg gaagactaaa tacgaataat
     4081 gtcgtacgac aagtaacggg taaacttact gcatctaata tcacattaag attaaatagt
     4141 gaatggtcag atgaaattgc taaagagatg tgtcacgttc actggattga ccagtcagga
     4201 aatgatttga cggatcaagt ggttgttcta aaaaacgaag tggatacatc taactttggc
     4261 gattacgaag tagtatttgc atcacctgat ttaacagatc aaatctcagt tacagtgacc
     4321 gtacctcgat tacgtaaact tacgctagga aaagaaacag caaccattga tattggttcc
     4381 gcatgggata gtgtcatagc cattaatact tttcaagtga aagctataga tgattttggg
     4441 aatgatgtta caaatcaagt acaggttact attaatgctg atatagatac aattaattca
     4501 attcaagcag tgattaagtt tgaagttcca gaattaggct tagcccaaat tggtagactg
     4561 aatattactg caaaaaatag tacaatcaat ggattgaatg aagttagcat cgagcacgta
     4621 aaaaagatta tgtcgttttc gtagaagaat ttggacattt agagggtgat acgattgtcg
     4681 gcatccacca taaaagtg
//
LOCUS       DAFCNS010000122         4634 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFCNS010000122 DAFCNS010000000
VERSION     DAFCNS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.128"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            174..518
                     /locus_tag="LNE23_002688"
     CDS             174..518
                     /locus_tag="LNE23_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBK6932497.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="LNE23_002689"
     CDS             519..938
                     /locus_tag="LNE23_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBK6932498.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="LNE23_002690"
     CDS             complement(1030..1380)
                     /locus_tag="LNE23_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932499.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2677)
                     /locus_tag="LNE23_002691"
     CDS             complement(1346..2677)
                     /locus_tag="LNE23_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBK6932500.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="LNE23_002692"
     CDS             2933..3499
                     /locus_tag="LNE23_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6932501.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="LNE23_002693"
     tRNA            3644..3731
                     /locus_tag="LNE23_002693"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4634
                     /locus_tag="LNE23_002694"
     CDS             4065..>4634
                     /locus_tag="LNE23_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBK6932502.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tgga
//
LOCUS       DAFCNS010000123         4594 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFCNS010000123 DAFCNS010000000
VERSION     DAFCNS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.130"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            189..2858
                     /locus_tag="LNE23_002695"
     CDS             189..2858
                     /locus_tag="LNE23_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBK6932503.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2949..3569)
                     /locus_tag="LNE23_002696"
     CDS             complement(2949..3569)
                     /locus_tag="LNE23_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6932504.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3683..4222
                     /locus_tag="LNE23_002697"
     CDS             3683..4222
                     /locus_tag="LNE23_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932505.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4219..4557
                     /locus_tag="LNE23_002698"
     CDS             4219..4557
                     /locus_tag="LNE23_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HBK6932506.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1423 a          790 c          860 g         1521 t
ORIGIN      
        1 aaaattttta agggtgggac ttttgattcc tacccttttt ttgcgtttat ttttgttata
       61 cgaacaaatt atgaagttat ttattctaaa ttttattttt ttgtgaaaat tagactcttt
      121 ttaagcagag aaaaacaagg atatgataaa ctgaatacaa cttcatagaa atttggtgga
      181 aaataaatat gaaaaaaaat ttaaagtcat tctttagaga aaacggacgc tacgcagcag
      241 ctagtttcct gattccgttt ttgattttag cacttgttta tctaagtatc gggatctatc
      301 ctggaagcag cagaagtatt ttggcaagtg atgccttttc acagttttct aattttcatg
      361 ccagcttccg aaatgttctt cttggaaagc aaagtatttt ttatacatgg aatgcttctc
      421 taggattgaa ttatttatct ttaatttctt actatctagg cgggatcttc acgccactgg
      481 tgttcttttt tccaaaccag atgatgccag atgcactgta tgttctgact ttgcttaagg
      541 tcggctgtgc cggattggct ttttggtttc ttgcacgtac gttcaagatt cctcgctggg
      601 ggcatgttgc acttagcgtc agttacgcat tgatctcatt tatcactgca cattcggaga
      661 tcatcatgtg gctggatgca tttgtttatc ttccattagt gattttgggg attcatcgtg
      721 ttatggatct taaacggccg aaacttttat ttatcagtta tttactcttg tttttatcca
      781 gtttttatat gggttttatg attggcgtat tttctttctt atattttatc gcccgtctat
      841 tgacaaactg gtcagaatat aaaaaaacga ttgttcctta tggtatcact tctttactgg
      901 caggcggcgc ttcaatgatc attgtattgc cagcagtgct ggatcttcgg tcaaatggcg
      961 aggcactgag tcagatcact acgttgaaaa cagaagcgac ttcttatctg gatctaatca
     1021 tgaaaaatat gattggtgtt tatgacacga caaaatacgg atcgattccg ttcgtttacg
     1081 caggtctgct accattagtc ctctgtctgt tttattttgc cagtcggaaa gtaccgttga
     1141 aaaataaatt tttgtacggc agtcttttcg ccatacttat cgcaagtttc tatcttgttc
     1201 cattgaattt gttctggcat ggtatgcatg caccgaatat gtttttattc cgttatgcct
     1261 atctgttctc tttcttagtg ctcctgcttg caggaaaagg atgggaaaag atagaacaag
     1321 acgatcgtgg gctgttgtta ggaatcattt tggtcgtagc tgctctcttt gcggtggcat
     1381 ggggcatcaa acctgaagga agctacacct atgtgacgat gacttccttt gtattgacag
     1441 tggttttctt agggatttat gcttttgctg tgggatttta tcagcataaa aaactttcat
     1501 tcaatcatct gagtatattg ttattattga tcatgtctgc tgaagcactg gtcaatacga
     1561 acagtatgat ccatggcatt cttgatgatt ggaattatgc ttcccgcagt ttatatactg
     1621 atccttatcc tgacttgaaa gctcttgtaa atgaaacaaa aaaagattcc gactcgtttt
     1681 accgcttgga aaatctggat ccagtatctg caaacgattc gatcaactat ggttacagtg
     1741 gtatcagttt gttttcttct ataagaaatc gtaattcttc ctcttacctg gataaattag
     1801 gtttccgttc aagagggaca aatttgaata tccgttataa caataatact ttgctaatgg
     1861 atgggtttac aggaatcaaa tataacattt ctaaaaacga aagcacgttc agtaaatatg
     1921 gcttccgcca agctggtcaa agtggcgact atacactgta tcagaatgtc aatgcattgc
     1981 cactagcatt tactgcaccg ttgtccgtta atgatgttga gcagccagtg acagataatt
     2041 tgaccagtca gacaaacttg atcaatacat tgtctggttt aaatgaacag tattttacat
     2101 tctatacacc tactttgaag aatcagttga atacgacgat cacaaagaca acgactggcg
     2161 tcacttactc agaaacagca gataaccagc caaaagagct cacttgggaa gtccatgtgc
     2221 ctgcaaatac acaagcttac ttaagtttgt tcccaaccaa ttttgcacaa ttagaaagtt
     2281 ctagtgcgac gatcactgtc aatggtacat cgcgaaaaac gcaaatcaac atttctggtc
     2341 aatactatga tcttggctat tatcctcaag agacaacagt gacatttact gcaagttttt
     2401 atggaacgaa agaagtaagt ttcatggagc ctaaagtcgt tggtctagat gttgtagcct
     2461 atcaagccgc aatgaatcag atcaaagaaa aaggtgtcga aatggaagcc actggcagaa
     2521 aagcttctgg gactgtaaat accgctgaag attccatgtt ggtgacaact attccttatg
     2581 acaaaggatg gaaagctaaa attgatggga aaccagtcaa ggtcagcaat ttcaaagacg
     2641 cattcttgat gataaaagtt ccagcaggta aacatactgt cactttcagt tacttgcctg
     2701 aaggatttac gataggtgca atcctttttg tcctatgtat tgcattgttt attctttatg
     2761 tctggtacct tgaccgcaat cgcccctttt atggcacgat tgatccagca atagaagaaa
     2821 aaccgcgaaa tacacacaga agaagacgaa gaaaataaag aaacgatgga atctgtgtca
     2881 ctcttctatt taaatggcaa aaatttcaat ctttaaatga caaaaaccac ctgatttttg
     2941 gtggtttttt atatacctat gactaaacta tctcttgctg cttcgacaac ttctacagtg
     3001 atcttatcca tttgattgat tcttaaaatc cgatcaatct gcgcaaataa acgatgaata
     3061 agcctaaaat ttccttttgt gatcttaatg atagttgtga tagcctcata atcagaaaaa
     3121 tcttctagct tgatatcaat tcctaaatct gcccatttgt attccaaaat atgatgtgtt
     3181 tcgtccttac taaggttatc aaattcatgt gcaaatccta tgcgagagta aagctgcgga
     3241 taacgagaca atcttttttc aataccaggc ataccgataa gaaccattgc cagatcattt
     3301 ctatcgtaaa tatctcttag ctgttctaaa tgctgaagct ttaaacgatc aatttcatcg
     3361 acaataatta agtcaattgc ttcataggtt ttaggcacag gaggatcttc ttgattgtat
     3421 tttagtacca tatgcattgc tttagtcaga cctattctac tagttattgt gttaatatcc
     3481 gaactgacac gagacatctt ttcagctggt acagtataaa aaactttatt tacacttaat
     3541 atttcatctg ttgcatttat caagaacaag aaaaagtctg aatattcaaa aaaaataacg
     3601 gtgtacattt ttaaaaggag atgtataatc gagatttaga aagcgttttc ctcaaaattt
     3661 aatgaattag gaagtgatta ctatgaaaaa attatttagc gcacttttta ttctgttatt
     3721 tggctttatt ggtattggga cttctggaaa tatagttaaa gctagcacga tggttgaaca
     3781 aacaagtgaa atggcaaaaa cttttagtca attaacacca gaagaacaaa gttacttcaa
     3841 atccaaagga tttagtgata aagatacttt ttacactcaa aagatagagc ctcaagatcc
     3901 gaaaacacgt ttttctgcaa atgtattgcg gttaaccgca tctactaaaa aaattactaa
     3961 tacgcagggg cggacagaat atattataac aagtcccaat tcggcgatgt atcgtatttc
     4021 tacacgcttg agtttaggta gtgcaaaatc atatactcaa acagtaaatc tagctaattc
     4081 ttatggttat ggtggaggaa tgtattttac atttactggg aaaagacaat attacagtgt
     4141 aaaattagct atgcaagttt ataatacatt tggtgtaggg tcagtctctt gcactgcggg
     4201 aggattgaca ataggaaaat gataatttct tactatgtct taaatagaag tgatgatgaa
     4261 ggactcttcg tttttcaatc attacatgag gatacgatgc taaaagagtt ttataatcgg
     4321 attaagaata aacagataga aacaaattta tattttttaa gtgactctat cttgcctttt
     4381 attagcaacc ctaaaattct taacttgttg cagacaaaag atttgaaaag tttaccgatt
     4441 attgtcattg atgaggaaat tgtaaaatat ggcgcttatc taagcgtaga agaattagaa
     4501 aaattaacta acattggaat gagtattcag gaggataatg gggtacatgg aacatagaat
     4561 gaatatgcac agttgagaac ggaatttgac attt
//
LOCUS       DAFCNS010000124         4477 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFCNS010000124 DAFCNS010000000
VERSION     DAFCNS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.131"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(832..1125)
                     /locus_tag="LNE23_002699"
     CDS             complement(832..1125)
                     /locus_tag="LNE23_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBK6932507.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1528..2706)
                     /locus_tag="LNE23_002700"
     CDS             complement(1528..2706)
                     /locus_tag="LNE23_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6932508.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2810..3124)
                     /locus_tag="LNE23_002701"
     CDS             complement(2810..3124)
                     /locus_tag="LNE23_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6932509.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3231..3446
                     /locus_tag="LNE23_002702"
     CDS             3231..3446
                     /locus_tag="LNE23_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932510.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3746..3988)
                     /locus_tag="LNE23_002703"
     CDS             complement(3746..3988)
                     /locus_tag="LNE23_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6932511.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3975..4403)
                     /locus_tag="LNE23_002704"
     CDS             complement(3975..4403)
                     /locus_tag="LNE23_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6932512.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1418 a          841 c          663 g         1555 t
ORIGIN      
        1 ataaaaaatc atcaaataat cctttacacg ttattctaaa tttaattttt attattttat
       61 tgatgtatag aaagtttggg gctttttttc tctttttatt ctaaggttat tttagtcttg
      121 tcctttaata caaagatttt atttttaaat aaaaaatact ctttttttac gattattgaa
      181 aaaaaaagat aaaacttgat taattgaagt attgcttaaa tttggaagct atgagggaag
      241 aataaaagag ttaggacggg attatctact atttttcttt gttttaaata tttgttatct
      301 ttgaacataa aagagaaatg tgtcgactgc agtgttgtgt ccctcatcac gaataaacgg
      361 tgttcaatta gctgatcttg tattcactta aaaaatggta aagtacgagt tgctattccc
      421 cattttcaat tttctaacgc tcaggacaaa atactgtttt cacaacctca ttggaataaa
      481 agcatatata ttgtttcaag aacgaaatct tgttatcttt ttaaagggca tctgtccaaa
      541 atctaatttt aggaggtacg ttttggtatt tattcgtaca aataaaagag aattatcttt
      601 attcatgtaa cgcatccaat tgattttttc atgtacgctc ctggaacgat tttactgacg
      661 attgcttatt taactgcgaa tcgctcatta gcttttatca tagctatcca tattttgaat
      721 aacgttcttg gctttgtatt atagatacag atgacaaaaa aagttcaagt atgcggtgta
      781 gaaccaatat cgcatacttg aacttttttc aatgagtata aattaaaact tctaattttc
      841 aattcccatc atctgatatg cccgaccgtc tgaacttctt ttgaaaaagc caaatgtgat
      901 tagataacgt ctttgttcga cataatcgcc actagaaaat acgttcagca gtatcgaatt
      961 aacctctgtt tctgtataag tcactccatg gtcaaagcgt ttcaagactt ctttcaatag
     1021 gatgtatttc ttttttgttg aacttggtac ttgttttact tttccatcat tcatgaatgt
     1081 gtctaaaact ttcatctttt cttgctccgt ataagtataa accatcttct ttctcctttt
     1141 gattctaaga atactccatt tatgttagaa tattctggat tttttattaa agcggttact
     1201 cttaagaaag atattatcat agtgctcttt tttttactac tacttatagg tagatttatt
     1261 ctaatccatt attctcccct accaataatt aaagaaaaaa ctggatcagg gtgttcaaat
     1321 tacatgctaa gaaaaaagaa cccttgaata ccaagggttc tttctcagtc tgataacaat
     1381 gattaacgac ggatttcttt gatacttgtg aaaagtcgtt aaagtattga tataaaagga
     1441 tttttattac ctctgactac atattgacta atttgttaaa aaatcgtttt tcaagaatcc
     1501 tgtaatacca gtgctttcac cgttttttta actctctaaa gaactaaaag caatcagagg
     1561 ttttaccttt tcatttgacg ttaagtggcg ataaattttc gaagttgttt ggtcagtatg
     1621 gcctaagcga tctttcaaga cttcccagct acagtgttcc gctactaagc ttgccattgt
     1681 atgacgagct aaatgagtat gaaatggctt attaattcct gagcgattca cgcaccgttt
     1741 gacgtgatca tttacatact ctcgacgata aggaacacca cgtttactag aatcaagata
     1801 cacaaacaaa tatgtttctt ctataataaa cctgtaatgt tgcctcatct cacccaaagc
     1861 cttaatcaac tgttgtagct tttccataac tagaggagtt actagaactc gtctagcaga
     1921 actaactgtc ttagtagttt caattcgaaa atccttactc ttacctttaa ctgccgtttg
     1981 cttatagaac agaatttcat ttcttttgaa atctatatct ccaggctgta gagcaagcgc
     2041 ctcgctgact cggcagccag tgtaaaatag aacatggaaa agtacagaca tcggtaattc
     2101 ctcatgaaca atgccataat tcaaaaaagt tctcaattca tcgacagtaa gaaacttgtc
     2161 ctcaatatta tttatggctt cttttaaatc agttaccgtt aactgatatt ttggcattct
     2221 cacagacttc attggatttt cacttatcaa attctttctt tgacaatatt taaaaatcat
     2281 atttagaact tgaataagtg attgtaaact atttttggca tagccaacat ttgttccatt
     2341 aacgccacat tcctttaagg taaaaagcaa ttcttgaagg cactctggtg tcaaatcaga
     2401 caacttttta tcaccaatcc atcgaacaat aatttttaat tgttcctctc gcttatcata
     2461 ggttatttcc ttttgttcat ttaacgaacg gtaatattgg aaccattgct cacctacaac
     2521 actaaacttg tcattgcttg caacaatttt tttcttaagc ctttgttgtt tctccatttc
     2581 cagataagcg agttgcgcct cacgttttga attaaagcca cgtcttgttg ttcgatatgc
     2641 ttgaaagtac catgtgatcc taccagtaga actgatttgc ttcttgaatc gatctgtttt
     2701 ttccatttat tgattcctcc tattttgaag gaacccaaca agcagaaacc ctttggaaat
     2761 ctttctatct atgtcatcat tgtaccgcat cttagaaaga aaatgtaact tataaagatc
     2821 gaagtagtcg caatttttct cgatagtcat tcgaatcact ctttctcatc cattctaaaa
     2881 catcatcacg aaaatagtgt cctcgaatcc cgtaccaaca gggaggaaac tcaccaagtt
     2941 ttactaaacg gttcagtgta ctatcactta tctgaaatag tcgctttaaa tcagtatttg
     3001 ttaataccag aggtaaatga ctgactgcaa gcaatttttc cgtgtaatct tccagttttt
     3061 tttgtaaaat tgaatagaac tctatttcca gagaattaac atttgtttta acttccaact
     3121 ccatcaccct tctctctcaa aatcaaaacg accaccacgg taccccttaa aattgtaaaa
     3181 aaacactcca gtactccatc aaaacggact agaaaagagg aatttttaat atgaaacaag
     3241 gaattgcaga cataaaaata attaaagaaa ttcttgaaaa atctacggct aatgcaatag
     3301 cattcggaac aggaatcaac ttgagtacag taaaaaaact taaaagtgga gagagagctg
     3361 aagaaaaatt aaatcttgcc gatgctatta aaatcacaga atttggcatg aaaaatatgc
     3421 caaccaaaat cgaaatatgg aaatagtagt ttttctgatc tatcagctgt tttgccaaag
     3481 cacataggaa tctcacctcc ccccagtaat ggcgggccga tttggttacg gaagtatcat
     3541 tatcgtagtc gcgattctca tattaaaatt tgtcgcttaa aagctaacct tgtcatggcg
     3601 taactagaga atcaaaatct acatgtactg atagatttgc tattcatttg tcaaagagcg
     3661 ctaggctatt ttcagcctat ggaaatcctt tattttgacc cgataaaaaa cttccataga
     3721 atgaaaagga aagggaaaaa atactttaac gagttgtatc aaattcaagt atcttctcaa
     3781 tcaattttgt ttccagttgt ctacgcatat attcatctac atacataacc attccttttt
     3841 cttcttcata aactggtttc atggctaacc gtgaaatata attttgatat tttgacagaa
     3901 cgatggacat ggcaattgaa tcaccagaag ctgcggcttt gattgtcgtt agtggtatga
     3961 atggtatata tcgtttattt tgcttcatat ttgacaccca aatactttcc tagctcttcc
     4021 aaagccattt gtcgttggta ccaaacactg ccgagacttt tcccaataat atcgccaatt
     4081 tcacgatcat taagaccgat gaagaaggac ataaaaatga tttcttgtct ttgactaggt
     4141 aatgctgcaa tcgcatttgc caactcctca ttggaaataa agattttcat accaaggaca
     4201 ataaaagtac cactactatt caaatttggt tccaaataat agaactctgt acctaaccct
     4261 ttttgttcca atatttccat tgatatttgc cgttggcgaa atctagcgta cttcttttga
     4321 atatttaacg cctcattgca gactactttc ttacaaaaac tgtcaaacgt atgttgaatg
     4381 tgggtttcct ctgagtcgcc cataatattc tccccctcaa aaaattcatt caactcgttt
     4441 tacttctatc cctttccatt attactacaa tctagga
//
LOCUS       DAFCNS010000125         4469 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFCNS010000125 DAFCNS010000000
VERSION     DAFCNS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.132"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(484..909)
                     /locus_tag="LNE23_002705"
     CDS             complement(484..909)
                     /locus_tag="LNE23_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932513.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1283..2254
                     /locus_tag="LNE23_002706"
     CDS             1283..2254
                     /locus_tag="LNE23_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6932514.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVERERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2286..3683
                     /locus_tag="LNE23_002707"
     CDS             2286..3683
                     /locus_tag="LNE23_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6932515.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3842..4465
                     /locus_tag="LNE23_002708"
     CDS             3842..4465
                     /locus_tag="LNE23_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932516.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1573 a          717 c          909 g         1270 t
ORIGIN      
        1 aaaaaaccaa acgacgggaa agaaaaatta ctacgtgatg tagaataagc ctttctatcg
       61 tttggttatt ttatattcaa atatttgaaa taaataaatc tttactcttt gatttgtaaa
      121 ctgaaatgat ccactgtttg ttttactgat gaagcttgat caaaggaaga agctgagttt
      181 gaagttcata aaaatcagcc atagtgagtt ttgcttggat ttgtttatct tcttctactt
      241 tttacataat tttgaacagg ataagattca aatgttttaa atgaatcttt gactagtttg
      301 tgacagttgt cgcatatgct caagcttcct tggtcattaa attctgtatg gatatttttg
      361 ttgttattac atgtacaata atcaggacta tcgatcatcc atttcgtctc ctttacaatc
      421 atcttgcttc tttactatag cagaaaagaa ggggagaaac aaaagaagat ccaacagacg
      481 gcactataga ctgtagatgt tgccatcctc atccttcaaa cggtaattac cgatttttgt
      541 aaatatgact gttgtttctt ttttattttc ttttcctgaa ataatatatt cgtcatcttt
      601 ggtagtgatc ttgatatctg tcagcactgt tttttcttca gatgattcag aagaatcagt
      661 tccttgtgtc tgatacgtgc gtgtccattc cattttttta tctgtaaatg tcaactgtcc
      721 cgcggataaa tccatatctt tacgtacttt tagcgtatat acactgttgg tggtatctgc
      781 tttttccgct tttaagacag aagaatcggt tgtttcgtcc gaggattggt cagagccgcc
      841 aaacaaatca cagccagtca atgtcaaaag tcccgcagac aaaataaata gtacgattaa
      901 tttcttcaaa atacttcccc catttgatag gataaagaaa ttataccatt ggaatgtaaa
      961 acttcaaacg gatggaagag aaaaacgcac aaaacaccca caaatagagt aaattttaga
     1021 ataaaatttt aaaaaggcaa aaaatccgcc actttcagaa agcgttttaa aaaagtgttg
     1081 caatctttta aaaaaatgct atattaaaaa tgaaccggtt caaatacttg tttaagtata
     1141 attaatagtg agaggaagtt gctctactga agactttagt tatatggata ttgatgacaa
     1201 gcaacactat gcaaagaatg cggtcatcat aatcgaacaa caagaaagag aaatcaaatg
     1261 taactggcag gagtgagaat gtatggcatc gatccgtgat gtggctaaga tggctggtgt
     1321 ttcagtggga acagtttccc gttacttgaa tggtcagcaa ctgaaggaaa agaacatgaa
     1381 aaaaatagca gaagcgattt cagcgttgga ctataaagaa aacatcattg ctaaagggtt
     1441 gaagaataat cgaagcttct ctattggact tttgataaac gggatttcca gccgttttgg
     1501 gtcggaagtt gtctcaggga tcgaaagagt agctgaagaa aatgggtata gcttattatt
     1561 aagcgggttt gccgatcagc cagaaaaaat tgaacaaaaa attgaatacc tgatgaagca
     1621 tgtgatcgat gggctgattg tcttcttatc cgaagaagag tggtctggat ttgaaatgct
     1681 cacaaaaatg agtattccag ttatcgcatt gaattgtcca gaaggaccag caggtattga
     1741 tacaatatta gtgaatgatc gagaaagtgt agcaaaagtg atcgctcatc atggtgaaca
     1801 aggacataaa aaaatcggat ttattgctgc cactcaaaca gactacgtag aaagagaacg
     1861 tttagcagga gccaaagaag cggtccaaaa tgatccatcg atccaattag aggtctttac
     1921 aggagactat tctagaaaaa gcggctatta cggggcaaaa gcattattag aaaaaggggc
     1981 aactgctatt tttgtcagca attacaatat gtcgattggt gccatcgaat gtttcaatca
     2041 agaaggtgtc agaatcggga aggatattgc gttcagtcac tatgattatt tagataaaaa
     2101 cgatctgagt atccttccga aaatcacgat cacgccacca acagataaaa tcggggaacg
     2161 agcagcagaa cagctgttaa aacgaatcaa aaaagaagag gaagaaaatg aaaaagtgat
     2221 tgtgatggaa aatatcatac atggaatcaa ctaaaacgaa ctaaaaaaga aaaagaggtg
     2281 ctttgatgaa aaaatatcaa tttccagcaa atttttggtg gggttctgca gcaagtggcc
     2341 cacaaacaga aggaaggatt tcaggcgatg gaaaaggaga gaacattttt gattattggt
     2401 acaaaaaaga accgcaaaag ttctttgatc aagtaggacc tgaaaaaacc tcgcaagttt
     2461 atacgaagta tcaagaggat gtacagttga tgaaacagac tgggcataat tcatttcgaa
     2521 catctatcca atggagccgt ttgatacctg atgaatctgg aacaatcaac gaagaagccg
     2581 ttcgtttcta tcatcgttat tttgatgagt tgattgaaaa cggtatcgaa ccctttgtga
     2641 atctgtatca tttcgatatg ccgatgtatc tgcaagaaaa aggcggatgg ttgaaccgag
     2701 aaacggttga agcctatgaa aaatatgcca aaagttgttt tgaattgttt ggttcgaaag
     2761 taaaaaaatg gtttactcat aatgaaccaa tcgttccagt cgaagcaggt tatttatatc
     2821 gatggcatta tccagaagag tcagatatga aaaaagcgat ccaagttggc tatcatgaag
     2881 cgttggcttc agcgttagcc atcaaatctt accatgaaat ggagcaaggt ggtgttattg
     2941 gtatcatctt gaatctgaca ccaagctatc caagagacga atcaaaccct gaagatgtta
     3001 aagcagcgca aatagcagat gcgttcttca atcgttcatt ccttgatcca gctgtaaaag
     3061 gcgagtttcc aagtgaattg attgagatag taaaagaact tgatctcatg cctgtgatcc
     3121 atgcagagga tttgacggtt atcaaagaaa atacagtaga tcttttaggt atcaattatt
     3181 accagccacg ccgggtaaaa gcaaaagaaa caccaatcga tactaaaaac ggtccaatgc
     3241 ctgaggatta tttcgacaat tatgaaatgc ctggtcgtaa aatgaatcct tatcgcggat
     3301 gggagatcta tgaacaagga atctatgata ttttgatcaa tgtacgagat cattatggaa
     3361 atatcgactg ctttatttct gaaaacggta tgggtgtaga aaacgaagca cgatttatca
     3421 aagaagatgg catgatccac gatgattatc ggatcgaatt tgtccaatct catctgaaat
     3481 atgtccatca agccattcaa gaaggtgcca attgtttagg ctatcatatg tggacatgta
     3541 tggataactg gtcgtggacc aatgcatata agaatcgata tggctttatt tcagtagact
     3601 tagcacaaga tggaaaaaga acaatcaaaa aatcgggtca ttggttcaaa gacgtatcag
     3661 ataaaaacgg atttgaggct taagaaagaa tagttgagag caaatgcgtg aaattctcca
     3721 ttcttaccat tttgtgaaaa agaagttcaa ttatttacga tctttttcaa cttttttgtc
     3781 tttctatttc attttttgta tataatagaa tagtcaaaat taaataggag tggaggaaag
     3841 catgaggaaa aaaagaccgt ttcttctctt ttggttattc ggttttttct tactgcttat
     3901 tttatccgca gcattttcag gaaaaatgac agcagatctt gctcagaaag aaacagaaaa
     3961 aatcgtagca agcaaagaat cagtagataa tttcaaaaaa gaattctcaa cttattggtg
     4021 ggcggagtat aaaataactg ccaattacga aaaaggcgaa ttggacattt atttgccaaa
     4081 aggaaaagaa gaaaatcaat ttattaatat cgatgacgta cttagtgcga tttctctcag
     4141 acaatatcaa tttaaagaca agcatttgac cgttttgctt ttgactcatg atggcgacaa
     4201 agtagcccgt ttgaaaaatg gaaaagtgat caccgcttat tctgaaaaag tagacaaaaa
     4261 agtggtcgcc gatcgtttag cggaatggaa aaagacaaat gaacaagtca agaaaacaac
     4321 tgtctcttct caaaaaaacg agtcgacagg aacaagtcct tcagattctg gcagtggaca
     4381 aacgataacg ggaagtgaag aaaatcaagc ctcaattatt acgaatgagt catcacaagg
     4441 acaatatcaa tcctatggac aatgaaaaa
//
LOCUS       DAFCNS010000126         4456 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFCNS010000126 DAFCNS010000000
VERSION     DAFCNS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.133"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            574..1008
                     /locus_tag="LNE23_002709"
     CDS             574..1008
                     /locus_tag="LNE23_002709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932517.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="LNE23_002710"
     CDS             2298..3221
                     /locus_tag="LNE23_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6932518.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="LNE23_002711"
     CDS             3237..3776
                     /locus_tag="LNE23_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK6932519.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1661 a          666 c          756 g         1373 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat gttttctccg cctcccaggt tcttgaccat
     4201 tttctcttat aagtgggatt tttaccacgt tgagagaaaa tggtcaagaa cctgggaggc
     4261 ggagaaaaca taccaagcac cacaagcaaa aaaagttaga gtaacttcca aaaaagaaaa
     4321 attttttaga acaacttttg aaaatttaat tttgatttat atccgttcaa tttaaacatt
     4381 tgataaactg aaggctagta tttaaaatgt gtatacaagt ggtagcgtta aaaaaagtgg
     4441 aggtataacc tatgtt
//
LOCUS       DAFCNS010000127         4453 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFCNS010000127 DAFCNS010000000
VERSION     DAFCNS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.134"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="LNE23_002712"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="LNE23_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBK6932520.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="LNE23_002713"
     CDS             complement(2020..2922)
                     /locus_tag="LNE23_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6932521.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="LNE23_002714"
     CDS             complement(2919..3611)
                     /locus_tag="LNE23_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBK6932522.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="LNE23_002715"
     CDS             complement(3654..4295)
                     /locus_tag="LNE23_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6932523.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1378 a          950 c          768 g         1357 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctc
//
LOCUS       DAFCNS010000128         4116 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFCNS010000128 DAFCNS010000000
VERSION     DAFCNS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.135"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..85)
                     /locus_tag="LNE23_002716"
     CDS             complement(<1..85)
                     /locus_tag="LNE23_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6932524.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            155..316
                     /locus_tag="LNE23_002717"
     CDS             155..316
                     /locus_tag="LNE23_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBK6932525.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            335..1612
                     /locus_tag="LNE23_002718"
     CDS             335..1612
                     /locus_tag="LNE23_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6932526.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="LNE23_002719"
     CDS             1622..2278
                     /locus_tag="LNE23_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6932527.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..3654
                     /locus_tag="LNE23_002720"
     CDS             2278..3654
                     /locus_tag="LNE23_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6932528.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3784..>4116
                     /locus_tag="LNE23_002721"
     CDS             3784..>4116
                     /locus_tag="LNE23_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK6932529.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1629 a          455 c          727 g         1305 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa acaattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt tgggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg gagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     3601 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     3661 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     3721 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     3781 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     3841 acttatcaac ccgaatctgt agaggatatg caagatgttt taaaagatgt gtttggtccc
     3901 ttatttgaaa aaatgcttca aggagaattg aataaccatt tagggtatga cgcccattct
     3961 aaagagccaa agaaacatga caatcgacga aacggttatg gcaataagac gcttaaaacg
     4021 agctttggta aagtaaacat tgatgttccc agagaccggg aggcttcttt tgaacctgag
     4081 ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DAFCNS010000129         4066 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFCNS010000129 DAFCNS010000000
VERSION     DAFCNS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.136"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            86..1435
                     /locus_tag="LNE23_002722"
     CDS             86..1435
                     /locus_tag="LNE23_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6932530.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="LNE23_002723"
     CDS             complement(1563..2153)
                     /locus_tag="LNE23_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK6932531.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="LNE23_002724"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="LNE23_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK6932532.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaa
//
LOCUS       DAFCNS010000130         4033 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFCNS010000130 DAFCNS010000000
VERSION     DAFCNS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.137"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(241..390)
                     /locus_tag="LNE23_002725"
     CDS             complement(241..390)
                     /locus_tag="LNE23_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932533.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            852..1064
                     /locus_tag="LNE23_002726"
     CDS             852..1064
                     /locus_tag="LNE23_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932534.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1342..3141)
                     /locus_tag="LNE23_002727"
     CDS             complement(1342..3141)
                     /locus_tag="LNE23_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK6932535.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3265..3861)
                     /locus_tag="LNE23_002728"
     CDS             complement(3265..3861)
                     /locus_tag="LNE23_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK6932536.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1448 a          711 c          689 g         1185 t
ORIGIN      
        1 tattaaatat tatacagata ataacggaat attatcatcg aaacagctaa caaaattgtc
       61 ataaaaaacg agatggttga tattagaact tgtgtttttg tttaggtcta gagataaaga
      121 gagacaattt ccaaatatat gtaatgaata caaaaagacg tttgtcgcgg attaccgtta
      181 caaacgtctt ttattgattt tccgagatct catcagtcgc catttggaat ggttcactcc
      241 ttatctagat tcgtttaatg ctcggactgc ttgttcgatt gtaataccaa acgcaacgtt
      301 attttcgttt tgtgcatcaa tcgccataaa acggttagaa gttgtatcaa aagctacgcg
      361 gtattgcata tttgtttttg taaacgtcat aaaattcatc tcctttgtaa tgtaatttag
      421 ttcttgaaag tttcctttaa actttcgaac ttataccaag tttagcatcg tacgtttttt
      481 gatgcaatta aaaagcattt atcattttcc ttatttattt tttcttaaat ggtttagcta
      541 ttttctttta atccgataag tagcccaatc atcaaggtta caaatactat tgcttgaata
      601 taaatcatat gctcacctct ttataaataa cttagcaata taaagaaaaa aacgcacgaa
      661 caacatagga atataaacaa acgtttttgt cacaacgtct aagatcgtgt taaaaaatag
      721 ttatctaaaa agcagaaaga tactcaaaat aaaaaataaa aaaatggata aaaacgtttt
      781 caaatacaag gtaatttgtt attataaaat aaagaaaaaa caaaataaaa ataaagaaaa
      841 gagggaatga aatggtaaaa gcagaagtag gaagatctta tatatgcaaa ccgatcggag
      901 tatctcatga actaatcggc tgtattgaac gtacatatat caacacggct gtcgtgtatg
      961 ttgaaagtta tgaccaacga gactatcaac ttatcaatga aagcaagtat cgcatgctcg
     1021 taaaattctg tgatattagc gcacctgctg aattggtttc ttaaacagta tttttgagaa
     1081 ttcgcaagaa ctcccttata atataaataa agacagaagg gagcgagaag atatgccaaa
     1141 aagaaaaagt tcagggaaaa tgggaattat tcttgcagtg atctctgcaa ttcctgctat
     1201 tttagctatt gttcgtgcga tgaaggaatc aagagaaagt taagcacaca ttatgagcgt
     1261 caaacaaaaa acgcatatac ctgcggtggc aggttatgcg ttttttgttg tataaagact
     1321 tttttcatta acgatttttg attagccgac tcccaaaaca aaatttagca gtttatctga
     1381 ataagtgatt tcttcatctg tttgtttcac actttcattt agtagtccca tctcatcggt
     1441 tatgatacca tccacaccat aaaacatcat gcgactcata gcatctgaat cgttgattgt
     1501 ccatacatat acttcttttc cgtcttggtg ggcagaatta atgaagttac ggttgatcgt
     1561 actatattcc attgtcagaa aatcagcagg cgtcacaggg ggcccaacaa tattgaaagg
     1621 taagatgtag cccacataaa agtttggttc tgcttctttt aaatctgaaa cggtttggta
     1681 cgtcaaactt tgcaaaatat gatggttctc taagataact ttgcggtatt tatggacaaa
     1741 tcgttcgaca aggtcatccg aatctttttt agaggtcttg atctcaataa gtaatttttg
     1801 gtttagctta tgtgcttttt ccaaataaga atcaaaagaa caaacagggg cttgcactcc
     1861 gttttcagta actgtaagat ttgtcaattc tttcaatgta agatcctgtg gtgttccatt
     1921 tacccctgtt aagtgacgaa gatttgcatc atgcatcact acgaaatgac catcttttgt
     1981 ttcctgaaca tccatctcta cataatcagg atgatagtct ctgcttgttt tttcaagtgc
     2041 ttctaaagta ttttgcgccc cattttgggt actcacacct ctgtgagaga cagtcactgg
     2101 ttttgtctga acagctgaag tcagataatc catattgtaa agacagacgc caataccgaa
     2161 aaaaactgca aaaagagtca aaccagtgtt tttcagcgca gagaaacgca agttgggggc
     2221 ttgtgggata aaccacttag ggatctcagg caagaagcct tcgtcatcca tgaaatcaac
     2281 aatgatataa aaaatcccta cggtagacat cacgatattg aataataaaa tcccttgtaa
     2341 aagcgtcata gcaaacacag cgctgatcaa tgcatagtct gggaaaaatt gttcaaccat
     2401 actttgtgcc aaaataacaa cgatatatcc taaagaagaa aggagtaaaa tcgtaccgcc
     2461 aatcacaata aattggcccg tgatggccaa aagacgcgat ttggtcaatg accagctttc
     2521 ccgaatggct gctcgaaaag gacgatctcg tagaatcatt tccggtaaag caaaaatcag
     2581 tcgaatccca atatagccaa ggaaaatata aacgagtaaa aaggaactga cgatgatcca
     2641 acgattagca aaaataaaat ccataatgaa agcggggatt tttatttttg acagtagatc
     2701 agaattaaaa cttaatccgc tgatcggtag aatcaataaa aaataagcaa ggaaaaacaa
     2761 aaaagtaatg ggtctcactt tcttcagctg aagtatcgtc aagtgtaaga gttgcttcaa
     2821 agagattggt tctttctttt taataaaaaa aacactcatc aaaagaaaag taaattcgaa
     2881 ataaactaac agtaaaatcg tcatcaaaac tacgattaac gagagtagaa ccccaggatg
     2941 ttgagaaaaa attgaaggaa tattgtcata agacaaatag tcaattgccc ctcggtgtaa
     3001 aataaaacgg gtcgtactgc tcaataaggg taaacaaata aataaaataa agccatgcat
     3061 cagcaagaca tcacggaaat aagcttgtgt tccttttaaa aagtcccaag cattacctaa
     3121 gctacgtttc aaataattca tcctatcacc tgctcttcac attgtagcgc aaatctaatg
     3181 aaaaaggaaa gggtgcagtg tgttttgcag accctttccc agatttaaca ttgtcaatca
     3241 atgactaatt ttacaaaata aatattatcc aagaagacca cttagatatt gacctccata
     3301 aatcaaggcc atactatata agaaaatcga cagtggttta gcaatcaaaa tgattagtgt
     3361 atatcgtttc actgacatgt ttgtcaatcc agccatcaaa cataacgcat cgtcaggagc
     3421 aactggaaga aaaatagcta gagcaaacag cttctcaaaa cggttttgat tatccagcca
     3481 gccaatatat ttgttatatg tcttatcgct aatcatggaa agtatgaatg gctttccata
     3541 acgacgccct agcagaaaaa tgataatcga accaatgcag attccaatat agttatagat
     3601 aaatccagca taagggccaa atatcaggac accagctgca gtactgatcc ctccaggaat
     3661 gattggaata actacttgca ggatttggat gaagacaaaa ataatcggtc ctaaaatgat
     3721 ggaatcacct actaatcctc ttaacgcatt caaatctttg aaaacaccta aattaataaa
     3781 ataaatcgtc agcgcgattg ataggcctaa tccgataatg gatatgaaat ttatcagttt
     3841 tcgagaggca gcaatattca ttattcatca actccttaga ggttctttgt taactatact
     3901 ttactagaaa agcaactaac tgcgccattt tttgatatga cagaaaacaa acaattttgt
     3961 aagttcatca taagtataaa agattgatct caaaaaaaca tccaactaaa gacttattta
     4021 ataacttgtt ttt
//
LOCUS       DAFCNS010000131         3900 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFCNS010000131 DAFCNS010000000
VERSION     DAFCNS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.138"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            45..380
                     /locus_tag="LNE23_002729"
     CDS             45..380
                     /locus_tag="LNE23_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932537.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(637..2220)
                     /locus_tag="LNE23_002730"
     CDS             complement(637..2220)
                     /locus_tag="LNE23_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932538.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            2342..2926
                     /locus_tag="LNE23_002731"
     CDS             2342..2926
                     /locus_tag="LNE23_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932539.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2937..3182
                     /locus_tag="LNE23_002732"
     CDS             2937..3182
                     /locus_tag="LNE23_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932540.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            3188..>3900
                     /locus_tag="LNE23_002733"
     CDS             3188..>3900
                     /locus_tag="LNE23_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932541.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQS"
BASE COUNT         1375 a          527 c          624 g         1374 t
ORIGIN      
        1 caatttccta ggaaaaatag ttaacataaa aggaatgaat tgttatgaaa caatatttaa
       61 atgaatggaa agcgatagag gggtcattag taacgcaaag aataaaggaa ttacctgatt
      121 gcttagagaa ggatcatttg tttcaaatac gagaaatgtt gaaaaaagaa cagtttgatc
      181 cagaccaatt tttagtagtc gagtatccaa ctattggagt gtactgttgt aaccatatca
      241 atgatggaaa atattttatt attcaggagt atggaggaca gttaactcct ttctatacta
      301 catgggaaat gagtgaagaa ggaatcaata attttccatg tgaaagcatc gaagattcaa
      361 tttcacacac cgaatgttaa ttgattataa ctataaaaaa ggattgaaca ttatgttcaa
      421 tccttttttc aatgaaatgt tttccgccat ttcattgttt ggtgcgattt ctattttatt
      481 atatcaaaat aaagagagat tctaaagaaa gaagagggaa atatataaat caagcgacag
      541 tatatttatt attaaatgct attgtgagaa tataactaat tagtttaatt tgaaagtaaa
      601 tatacgctat aaaagtgcat cttgtgttat aaaaaattat tttattttgt gaacataatt
      661 taacaattct ttctcggaat tgatttcttt tcttaaatag tcttcagact tattaataat
      721 attatctatt atgtctagat acatatcact tttcgattca gctaattcat acagtttttt
      781 taattcttta tcctttaatt gaaaaccctg atccatagtc actgcatttg gatatagaag
      841 cccactataa tgtttacgta aattcacttg gaatttctga ttataaaagt ctaacagatt
      901 agaaataaaa ggcggaaaat tactgttttt gtaaaattga ttatttccat ttaggttgta
      961 tagtaatttg ttaaattgct cctgatttaa tttattgtaa atccatggta atacttccat
     1021 atccttatct gctaacctga atgaactagc atcggtaaga tgttcgatca taaaatctat
     1081 gaacatctct ttttcaccaa aatataaata ttgattatat attgtttcta atgttgaaga
     1141 tggtagcgaa gaaactctca ttttttgctg gagtatagga tcaactagac tatttaagtg
     1201 attttgtaat tcttcattgt tgtttcctag aactgcaagg gaacgtaaac ggtagtaata
     1261 ttctatttct gttagtactt gttgtttcgg tttaaaatca tttattgata tttgtaaatt
     1321 ttgaattgtc tcgtctgaaa aatcatgttg taattctgga tgggtagcta taaaagtaag
     1381 taaaaagaac ccttttgtat acgtgatctg ttgattttca ccaagtgatt tattaatttc
     1441 ctctcttgat tcagctgtga acctatactt tgcaatttct ttttttagaa tatttaaata
     1501 gaattcttta ttatcaaaaa ttagggcatt aagtagtctg aaagtacttt ccctatctct
     1561 atctgcttcc tcatttccat aagataattg aaaaacaaac ttaaaggctg tcttacatag
     1621 gttttctttg gctttttcgc ttagtttatt tagatagcta ttttttagtg cagagtgaaa
     1681 ttcttcaggt gtaacagaca tagcatgtat gcgacttgca tattgtataa tttcgatcgt
     1741 tacttttttt cctaatattg gattttttag gaatataatt tcataggact tccgtataac
     1801 atcctgacaa gtttcttttg ttatatctct taattcccaa gtattaccgt tgtaagtgac
     1861 tacaggatga gctgaataat ttctttgatc cttaatatat tttaaatcct catattcata
     1921 gctatcaatt agctcagttg aactataagc tttttctagt agttccattt cccattttgg
     1981 agagtttgga tcactttttc ttagtctagc tatatcttct aaaattttct tagaagtatt
     2041 atcctcgtaa atatttgcta aatcagataa tttatcaatt aaatctaaaa taagcgcact
     2101 gtaattgaca gctattgcag aacgataatt tccctgataa aaactacttc taacttcttc
     2161 taataatgga ataattctag gatttttaac tttttttatt tcatcatcta atataaacat
     2221 ataaatctcc tttttttatt tgtatgacaa gtttaccata aaaaaaaatt aaaaagcgag
     2281 acatatttat gttgttgtgt tatcataata acataaatat gtagagtgag atgatgaaaa
     2341 aatggatttg aaacaatcgt tttacatctg gttatatcct gataatccta tgtcaataat
     2401 ggctgatatt tttgtagtta gcatcttatt gtatttattt tatctaattt tctttcacaa
     2461 tttaatcggg tacatgattc ttgattggaa aggaatgttc gatatagaaa gaaaaccatt
     2521 tgctaacaat caaacctata ccgtcttcga aagtggagat tgggaagaaa atgaagttga
     2581 tgtgatccat gaaattggat tagctttatc aggaatacaa gatgctgatg ttttggtatc
     2641 aatgacaacg gatactggat acaacgctta tgaggtattt tatcttatgt attttatatg
     2701 gcgtgatggt caagaaatta tcaatggtcg tgctgaaaaa acaatcaatt ctaatactga
     2761 aatttctttt tgtatgggtg aaggggaaat gattgtatat aaagatgggt cagcaaacat
     2821 taaaacagcc tttggtgata ttgaaacaag tattaaggaa cttagggaat atgtgcagaa
     2881 cccatataat tttcaatatt tagcaggaag aataaattta gtttaggagt aaaaatatga
     2941 aaaaaattat aaatcaacca acaaatcact tgataattga aggtgttatt ttcgtaacaa
     3001 tgtattttac tgtaggaaat ggcatctcaa atacaatcat tcaaaaaaat ccacaatacc
     3061 tattatttag tctttatgct tggattgtac aaatggtttg ggtttccata ttgatgctgt
     3121 tgttttattg gatagtacgg gttattcttt tattgttaaa caaaagaaaa aaggataggt
     3181 gaataaaatg aaatcaacta aattgaaggt tgtattgtta tcgacagcag cattaataac
     3241 ccttacagca tgtggtacaa ctaatagtgc taataataac aaagacacaa agtctagtag
     3301 tacgatggtt actaaaaaca gcactacatc ggcatcaaca acaatgtctt caatgactga
     3361 aaataatgct gaaaagtatc aaaaatacga agacattaat aaaggtcaaa agccggatga
     3421 agtttataac aactttatta atacagtaaa aaatgatgaa aattatggga atgatccatt
     3481 gcaaaataaa aatctgacta tcgttcttta caaacctgat tgtccagatt gtcaaaaagc
     3541 agaatcaaaa atcatgcaag aaataaaaga aggatttagt tatgctccaa cattaatgca
     3601 tgatattacc ataaataaac cagcttacga gtattacgca gttgctgtag atattactga
     3661 tggtgtacct gagtgggtac aaaacattcc tggtctggct gatgagaaca atcaatatca
     3721 tacgccaaca gttgcagtag taacaccaaa gaaagttaac ggcacactat actgggatgt
     3781 aatgggaatg acaacaaata caactgatga aatgattgaa agtacaacta aatattatat
     3841 ggatttggga gcatataaat tatatcaaca atcagagcct gttatggacg atcaatccaa
//
LOCUS       DAFCNS010000132         3857 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFCNS010000132 DAFCNS010000000
VERSION     DAFCNS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.139"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1812
                     /locus_tag="LNE23_002734"
     CDS             <1..1812
                     /locus_tag="LNE23_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932542.1"
                     /translation="TQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEI
                     KGRVDSLNQQITDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPN
                     LMPYITEPWVGPLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRY
                     LITTLRPSTTYTLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERD
                     KMVTHTFTGITKDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWL
                     GKVPLGENIADPTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYI
                     AAGRVKVGDMKPTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLK
                     LEKGNTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANN
                     YTDGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLT
                     VTNGNTGSAKLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSID
                     RSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            1829..1978
                     /locus_tag="LNE23_002735"
     CDS             1829..1978
                     /locus_tag="LNE23_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932543.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1975..2421
                     /locus_tag="LNE23_002736"
     CDS             1975..2421
                     /locus_tag="LNE23_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932544.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            2423..2560
                     /locus_tag="LNE23_002737"
     CDS             2423..2560
                     /locus_tag="LNE23_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK6932545.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            2598..2891
                     /locus_tag="LNE23_002738"
     CDS             2598..2891
                     /locus_tag="LNE23_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932546.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2888..3112
                     /locus_tag="LNE23_002739"
     CDS             2888..3112
                     /locus_tag="LNE23_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK6932547.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            3109..>3857
                     /locus_tag="LNE23_002740"
     CDS             3109..>3857
                     /locus_tag="LNE23_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK6932548.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWH"
BASE COUNT         1351 a          646 c          802 g         1058 t
ORIGIN      
        1 acgcaagata tcattttttt ctcactagga gtgtctgaca tttcgcaagg ccaagccgat
       61 gaatatgttt cgcaattaga agagttgatt cgaaagtaca acgaaacttt tgatgctttt
      121 atggctgaaa tcaaaggtag agtggatagc ttaaatcaac agattactga tttaactggt
      181 caagctaaaa cgctacaaga caagttagat gctctgaaag aagaaatttc taagttaggt
      241 aacttacaag tgatgtacag taacagcatc gacttcgggg actatgatta ttcggagaac
      301 ccgaatttaa tgccatatat tacagaacct tgggttggac ctttgttagg taatggacat
      361 acagttaaag atagtgttaa aagggttata actcatacta aaactagaac agctaattcc
      421 ggcgacatat tatcgcttgg cttaggtatt ccgtgtacag cggaagctaa taataggtat
      481 cttataacca ctttacgacc atctacgaca tacacattaa gcgttactat gagtgtcggt
      541 tcagattgga ctggagagac caatactata ggggttaggc ttagatattt aaatgaacaa
      601 ggtggtatag aactgcccat taatgcatta ataccagcaa atgtagaacg cgataaaatg
      661 gtcactcata catttactgg tataacaaaa gataatgtaa ctagcataac aaactgttat
      721 gttgaaatat tctcacttaa tagtgaatat aaaggaacgg ttagtgtcag ctatgacgtt
      781 aagttaaaag cacactaccc taacttacta gatggccctt actggttagg taaagttcct
      841 ttaggcgaga atattgctga ccctacagtt gtctttccac acaaaactag tgaatatatg
      901 gtttatggta gacgcaatac agaaaactat atcgctgacc aaacatatac catatcaatg
      961 aaagcaacta aactaacagt tcaatcgttt gccgtgtata ttgctgctgg ccgcgttaaa
     1021 gtaggagaca tgaagcctac tgaggggtta gctaatacat gggagttaac ttttactgta
     1081 actaagcaac atatagatag tggggtaact aattatctgg aaatatacca atacccgtca
     1141 gctacaaaag gagctgttca aattgagtgg ctcaaactag aaaaaggcaa tactcgaacc
     1201 ccgaatatta gtgagtataa atatcgtggt actggtatgc gtgattcaaa caatccaaaa
     1261 gattatgttt gggatctagc accagaatat gtcgaagata atttggccac agatattaaa
     1321 atttctgaaa ttactggtaa agcaaacaat tataccgatg ggaaagtatc ggagattaat
     1381 tcgcagttga ctgcttcaat taatgaagta gacaccacag ctaaggatgc tcaaacaaaa
     1441 gcgaatgcta atgcgactgc tatagatgaa ttagacaata agatcgatga acgcattaat
     1501 gatacagcta ctaccacatt aacagttaca aacgggaata ccggatcagc aaagctttat
     1561 cgtgaaggaa aaacagtttc tatatatttt gtggctttaa acggaaaaag cagtggtgga
     1621 aatgattcaa cgatactaac aattccagaa ggctatcggc caccaattag ttttgagcaa
     1681 ctggttggct cgatagaccg ttctactttg aacagtgctc agttatctat tggtgcagat
     1741 ggagccatta aatggcgaag aaactcaagt tatggatcgg attatacctt tgcaattact
     1801 tacacgattt agaaaagcgt gaatcgatat gaaggcagca tatagaccaa ttgaacctta
     1861 cggattcgag caaatcattg tgaatgatga agaacattta ccggaagaat gcacagaagt
     1921 cgaaccaccg attccaaatt ggaaaccgaa attcaattac tgggagggaa ataaatgaaa
     1981 aacatttgga aatatggacg tactggcgga gagtacgcag gaaaagtatt ggacgacatg
     2041 cttgtatccg ttccttacac agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa
     2101 ccgttaacaa tcgctgatca aatgtttgat cctaaattga atcaatggat tattttagcg
     2161 aacgcactag atcacaacga tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat
     2221 gagaacggcg atttaaaaca gatcaatgcc aaactcatgc taagcgatgt agcaattaaa
     2281 caggaaaata ctgcattgaa agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg
     2341 atgcttgctt cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca
     2401 gcttcaaagg gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg
     2461 gggctgtttc actaacgaac aagtaatggt tttcgttccg ttgtgcatta ctgaagaaaa
     2521 agcagataaa atcattagca aagaagagag cgcatcttaa ttgatgtgct ttttattttg
     2581 attcaaggag ttgtcacatg attaatttag gggaatgggg agcgatagca ggatcaataa
     2641 ccgctatcgt ttctttgatt ttattagtaa taaaaccaat tactgcatct ttctcgaaga
     2701 ttactgagac tctttcaaaa gtaagtcaca atttagattt gctgactaaa gatttagaat
     2761 cgagcaaatc agatcgattg atgattcatg aagaactaaa gaaacacgat gaaagattag
     2821 atacacatgc agaaaaattg gtagaacaca cgcaacaaat taaaacttta tttagggaga
     2881 gaagaaaatg aataataaaa cgttcgaagt actaaaatgg ttcgcactgg taattattcc
     2941 cgcactagct actttcgtgg ggttagttgg taaagcgctc aattggcagt acacagatat
     3001 ctgtgttgtc atcattactg gttttggcgc gtttttaggg agtgtgttgg gtgtatcaaa
     3061 tcgaacctac aaaatgttct cggctgaaag cgaagaagga ggaaacaaat gaaaaagaaa
     3121 attactatta ctgcgatgag cctgttaacg gctctttttt tattacccat taatacgttt
     3181 gcttatacta ttaatgacga gtataattta gcgccgaatc aaggagactc cagattagca
     3241 attcctaaca aaattatttt gcacgaaact ggaatagatg caccagcaag aaacgtagcc
     3301 gccaacatga aaaataatta taacggaagc aattcttata ctacagatgt tattggtgac
     3361 ggtgggattg tttaccgtgt gggtgagcaa ggatatgttt cgtggggagc tggtaacgct
     3421 aatccttatg cgcctgtaca gattgaatta cagcgcacat atgataaagc attgtttgaa
     3481 aaaaactatc gagcttatat tgaatataca agagatagtg caaaaaaata tggaattcca
     3541 ttgactcttg atcaaggaac ttctttattt acaaaaggaa tcatttctca tttgtgggtg
     3601 acaaattatg tttgggggaa ccacacagat ccatatggtt acttatcgca aatgggagtt
     3661 agcaaagaaa agcttgctta tgatttagct catggattta ccgatgaaaa tccaacaact
     3721 tctgaaaaca agcctgtcat tgatccaaca cgagctggtg cagctaatcc tacgctgaca
     3781 gatggaacaa atcacgccca cattgatcag tttggggaaa tcgaaaacgc aaacttgcat
     3841 gtggctggat ggcacat
//
LOCUS       DAFCNS010000133         3856 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFCNS010000133 DAFCNS010000000
VERSION     DAFCNS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.140"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(50..301)
                     /locus_tag="LNE23_002741"
     CDS             complement(50..301)
                     /locus_tag="LNE23_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6932549.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            complement(315..506)
                     /locus_tag="LNE23_002742"
     CDS             complement(315..506)
                     /locus_tag="LNE23_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932550.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            799..1119
                     /locus_tag="LNE23_002743"
     CDS             799..1119
                     /locus_tag="LNE23_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6932551.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1137..1559
                     /locus_tag="LNE23_002744"
     CDS             1137..1559
                     /locus_tag="LNE23_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK6932552.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            1642..2277
                     /locus_tag="LNE23_002745"
     CDS             1642..2277
                     /locus_tag="LNE23_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBK6932553.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            2395..3606
                     /locus_tag="LNE23_002746"
     CDS             2395..3606
                     /locus_tag="LNE23_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6932554.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
BASE COUNT         1417 a          618 c          682 g         1139 t
ORIGIN      
        1 ctatttcccc aaagccctaa agcgtagatg attagcggtg cgctgaatac tagtgctctg
       61 ttgaatttgc ccatgttttt cctcctttta gatactgata caaagcgata ctactaaacc
      121 tccactctct gccaactttc gctgctggaa tctttccaga ttcggcatct ttagttagcg
      181 tgcgtgtcgt ggttttcaaa tattccgcag cttgctttgt atcccacact tcatttgcaa
      241 tctcagattc tgccaaagaa gctttgaggg ctgagaggtt aactaaagct agttgttcca
      301 ttgattaatt cctcctatcg aattctgtaa tcacggatga tttctaagat agttttatta
      361 gcttttggtc cactccaatg gccatcaata atttgctgca ttcgaacacg tgtgtatcca
      421 tatgcagtag ccaaatcttc catagttact ccgttttctc gcataaactt cttgattgca
      481 gcacggccgt tatctagatt tgacatctat tcactcccct tacatataga tttgtaagtc
      541 aaaatgatag aaaaaacgta taaactattg actaatagta tacaatcgta tactatataa
      601 acatagttaa ataagcctac aacaaaccct ttattatgca atcggtcgcc aaacttaatg
      661 ctataaggtg tatttttagt ttgctttttt tctatcaaat taacttacaa acaaataata
      721 atacaaactt atacttatgt caacagtata atttacattt tgtgtacttt tatttgttta
      781 ggattggaga acattattat gacactgttt gaaaggataa aatcattagc tagccaaaga
      841 gataaaagca tgaaagaagt cgctttagaa ttaggattta gcgaaaatct tttctatcga
      901 tggaaaacaa cagaacccaa agcaagagat ttgcaaaagg tagctgacta tttcaatgtc
      961 tctgtagact atcttctagg tagagaagaa aaagaaacgc ctaaacatgt ggatttatca
     1021 gaagatgata ctgtattttc ttttgatgga aaagaaatat ctaaggagac aatgcgtaaa
     1081 gcgattgcaa ttgctaaagc tttagaggaa aatgaatagt tggagtgatg ggttgtatgt
     1141 atttaaagtt gaaagaaatg ctgagtgagt ataatttaaa gttaatctat atggaaatgg
     1201 aagaaccagg tttttattat ccaaaaccaa gaatagtatt tttgaatgaa aaactacacg
     1261 aagacagttc tgaagctttt catttagccc atgagctcgg tcatttcatt gcttcacatt
     1321 ttgaatattc agtactgtac gataactcta caacttttca ttcaaaattc gaagctgaag
     1381 ctgatagaat cgcaattatg attttactaa atatctttat tgagaacgaa ttgactgatg
     1441 aatctcagtt caaattagaa aatttcatgg aattctactc tatcaataat aagttaagaa
     1501 cagaatgttt taatgtttgc cagtcatatt tcaagaaaaa atactcttat gcacagtaaa
     1561 aaaagcccgt gctgcaacac ggactcatac ctcatttctg agatcacaaa tatattataa
     1621 caagaagtga gggatattta aatggcaaaa aaagttatgg gtcaagatgg gaaaatgtat
     1681 aaggttaaaa aaccgtttta taaacgggta tggttttggg tattaatagt acttttgttt
     1741 tttggtattg gtggtgctct aagtggcggt gaaaacaaag attcagctac tgcaacatca
     1801 acaagtacta ataaagaaac tacaaaagaa actacaacaa aagaagaaaa gacttataaa
     1861 atcggtgatg acgttgctgt agggaaaatg gaatacaaag taaactccgt tgaagtagta
     1921 aaacaagttg gcccatctgt acttcctact aacgcaaaag acactttctt agtagttgac
     1981 ttatctgtaa aaaatgcagg tgataaggct gtgactgttg atagctcatt cttcaaatta
     2041 aaagcagatg gaaaaacttt cgaagctgat tcggctgcat ctatgtcggc aaatcaagat
     2101 gaaaatggta atatcactaa ctctttcttt atggaaagct taaatcctga tatgcaacaa
     2161 actggtaaaa tagtttttga tatctctgaa gcccaagcaa atgcacaaaa taatgtattg
     2221 caagcacaaa caggttactt tggaacagag acagtttcaa ttgctcttca taactaatta
     2281 gtataaaaaa cacgccccac cgaccaaagc gagcgtgttc taagaaaaaa caaacctaca
     2341 caataggctt ctttatagta cctattgtat cagagaaaga gagctgattc aattatgtca
     2401 gaagtaacag gatacttaga acaagtagac aatgaaacat atagactaag agctgtatta
     2461 gggtacaaac ctgatggctc ggcaaaaaga aaaagcaaaa ctataaaagc gaatagtaaa
     2521 agagcggcat ataaagaatt gaacatctgg ttagagcaat tcgaaggaat gaccgatgat
     2581 tcgttggatt tattcaatat aacttttggt gaattttata gaaaaatatg gctttcagag
     2641 gccgaaaaaa atttagagcc aaagtcttat cataactata agcgaatgat cgagaataga
     2701 tttcttgata aatttgattt tatccctcta atcgatataa aaccatacat gataaagaaa
     2761 attgttgtta atgctcaaag aatcaataca aaagatcctg gaagaaactc tgataaacct
     2821 ttatcaagaa atacaaagct tcgtatgtta tacgccgtta acaacttatt tttgatggct
     2881 aaaaatgagt acggagcaat taaagaaaat cctgtggaaa atgtaaaaat accaaaagaa
     2941 aaaggcgtaa aaaagaatat cgaagaacct tattcggaag aagaaattca tgcaatgcta
     3001 aaagcagcat ttgaggaaag catagaaatt aaaactttaa ttgttctagc atttattaca
     3061 ggagcaagac aaggagaaat tgcagcttta gaagaaaaag atattgactt tgacaaacaa
     3121 gaaataagat ttcatcagcg gatttcagaa gtggatggaa aatcagatat tcggttgctg
     3181 ccaggtttga aaaatgatga tgatgaaaaa atagtaaccg gtcctgctta tctatttgat
     3241 atgttagatg aattgatcaa agagaataaa aaaattcgtt ggaaattaaa tataaagaaa
     3301 ttaagacact attttatatt tgatacaaaa caagacggaa ctttaccacg tggaagttat
     3361 ctgtataaga agttcaaacg attcaccaaa cgtcacaatt tgcgtcacat tcgttttcac
     3421 gacattagac atacatcagc tacctatttg cttagcgatc ctaatatgac tccaaaagag
     3481 cttcaaaaac gattaggaca ccgagatttt aatactacaa tgaatgttta tggccatgtc
     3541 ttacgcaaag aaaaagatac agcaactaca gcgtttgaaa aactattaaa aaaagatata
     3601 aaataacact aaaaaattat attttcgggt ataaactcgg gtaggatata taaaaacttg
     3661 ggataatttg cggtatataa gcatttaaaa atgctgtaaa ttcgcttatc ttcatttaaa
     3721 ataccattaa ataacgaact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
     3781 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
     3841 cgctgttctt ttttta
//
LOCUS       DAFCNS010000134         3816 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFCNS010000134 DAFCNS010000000
VERSION     DAFCNS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.141"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..861
                     /locus_tag="LNE23_002747"
     CDS             <1..861
                     /locus_tag="LNE23_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBK6932555.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
     gene            973..1869
                     /locus_tag="LNE23_002748"
     CDS             973..1869
                     /locus_tag="LNE23_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6932556.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1967..2176
                     /locus_tag="LNE23_002749"
     CDS             1967..2176
                     /locus_tag="LNE23_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBK6932557.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            2194..2466
                     /locus_tag="LNE23_002750"
     CDS             2194..2466
                     /locus_tag="LNE23_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK6932558.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            2468..3331
                     /locus_tag="LNE23_002751"
     CDS             2468..3331
                     /locus_tag="LNE23_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HBK6932559.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1541 a          580 c          654 g         1041 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gttcaacttt tggggttcag ttcataaaga gggcttttat
      901 atattaatca caaatcactt atcacaaatc acaagtgatt aatcacaaat cacttgtgat
      961 ttgtgattct taatgataca atattactat acaaaaaaag aatggggcgt agttatggag
     1021 aaggaaaaac taaaaatact tgaagaatta agacgtattt taaacaataa aaatgaagca
     1081 attattatct tgaataatta ctttaaaggt ggtgttggaa agtccaaatt atcgactatg
     1141 tttgcttact tgacagacaa attgaattta aaagttttaa tgatcgataa ggacttacag
     1201 gcaacattga caaaagactt agcaaaaact tttgaggtag aattgccacg tgtcaatttt
     1261 tatgaaggct tgaaaaatgg aaacttggct tcttctattg ttcatttgac tgataattta
     1321 gacttgatcc ctggcacgtt tgatttgatg ttactgccaa aattaactcg ctcatggact
     1381 tttgaaaatg aaagtagatt gcttgctact cttttagcac ctttaaaaag tgactatgat
     1441 ctcattatta ttgatactgt accaacgcca agcgtttata caaataatgc aatcgtggcg
     1501 agtgattacg ttatgatccc tttacaagca gaagaagaaa gtacaaacaa cattcaaaac
     1561 tatatttcct atttgattga tttacaagaa caatttaacc ctggactaga tatgatcggt
     1621 tttgttcctt atttagttga tacggacagc gcaacgataa aatcaaacct ggaagaactg
     1681 tacaagcaac ataaagaaga taacttggtt ttccaaaata ttatcaagcg aagtaataaa
     1741 gtaagtacct ggtctaaaaa tggaattaca gaacacaaag gctatgacaa aaaagtttta
     1801 tccatgtatg agaacgtatt ttttgaaatg cttgagcgaa ttattcaatt agaaaacgaa
     1861 aaagaataga atcacaaatc acaagtgatt aatcacaaat cacttgtgat ttgtgattgt
     1921 tgatgataaa ataagaataa gaagaaatag aaagaagtga gtgattgtgg gaaatttagg
     1981 cgcacaaaaa gcaaaacgaa atgatacacc aatcagtgca aaaaaagata taatgggaga
     2041 taagacggtt cgtgttcgtg ctgacttaca ccatattata aaaattgaaa cagcaaaaaa
     2101 tggcggaaac gtaaaagaag ttatggatca agccttagaa gaatatatac ggaaatattt
     2161 acctgataaa ctttaaaaag gagtgaaaat gaaatggcag ttacgtatga aaaaacattt
     2221 gaaatagaga tcattaacga attatcggca agcgtttata atcgagtatt aaactatgtt
     2281 ttgaaccatg aactagatac taaaaatact cgtttactag aagtgaatct tttaaatcaa
     2341 ttagaagtgg cacaagaagt tgatttattt caacaaccat ttgaagaatt acaagctatt
     2401 catgagtatt ggcggtcaat gaatcaatat tcaaaacaaa ttttgacaaa agagaaagtg
     2461 gcttaacatg gcgaatatta ctgacttcac cgaaaagcaa tttgaagatc gtttagaaaa
     2521 gaatgttgaa cgactaacta aaaatagact agcggttgaa tcgccaaccg cttttttact
     2581 tggtgggcaa ccaggatcag gtaaaactag tttgcgatcg gcaatttccg aagagacaca
     2641 aggaaatgtt gttatcattg ataatgatac gttcaaacaa cagcacccta attttgatga
     2701 actagtgaaa ctttatgaaa aagacgtagt aaaatacgtt accccttatt ctaatcgcat
     2761 gacagaagcg atcataagcc gtttgagaga taaagggtat aatttagtga ttgaaggtac
     2821 aggacgaaca acagacgttc ctattcaaac cgcaacaatg cttcaagcca aagattatga
     2881 aacaaaaatg tatgtcatgg cagtacctaa aatcaactca tatttaggaa caattgaacg
     2941 gtatgaaact atgtatgcag atgatccaat gacagccagg gcaacaccaa aacaagcgca
     3001 tgatattgtt gtcaaaaact taccgaccaa tttagaaacc cttcataaaa cgggcttatt
     3061 tagcgatata aggctttaca atagagaagg agtgaaactc tattcaagct tagaaacgcc
     3121 ttccattagt ccgaaagaga ccttagaaag agaattaaat cgtaaagtat cagggaaaga
     3181 aattcaaccg actttggaga gaatagagca aaaaatggtt caaaatcaac accaagaaac
     3241 ccctgaattt aaagcaattc aacaaaaaat ggaaagcttg cagccaccta caccaccaat
     3301 acccaaaaca cctaaacttc cagggattta aaccttaaaa aagtaaaaga gtagttacca
     3361 aaaaacggta actactcttt ttttataaaa acatttcctc attttttaaa agctgatcga
     3421 gaaataaagc acacaactgt attctttctt ttttcagttg gtacattttg cagtctgtaa
     3481 cgtgccattt actgtttgtt accttaacat atccagtcga aaacacaagg gtatataaac
     3541 gagcaagctc gcccttgtgt tttctttgtg tctatgttgc gttctcgtca cagcaaacga
     3601 cacgaacaca ctggcgaaaa tgaaccaaca aaacgaaaaa agaaaggagc gtacactgta
     3661 atttatttct agcttttaaa acatgaggaa aacagcttca cttcctcaaa cgcctaacaa
     3721 aaatggttgt caaaaaacta aaaatcaaaa aaagaaaaga ggaaaagaaa atggaaaaaa
     3781 caaaaaaatt acaattagaa gattttacag aaaatg
//
LOCUS       DAFCNS010000135         3785 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFCNS010000135 DAFCNS010000000
VERSION     DAFCNS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.142"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..171
                     /locus_tag="LNE23_002752"
     CDS             <1..171
                     /locus_tag="LNE23_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK6932560.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="LNE23_002753"
     CDS             406..642
                     /locus_tag="LNE23_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932561.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="LNE23_002754"
     CDS             649..2601
                     /locus_tag="LNE23_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBK6932562.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="LNE23_002755"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="LNE23_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBK6932563.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="LNE23_002756"
     CDS             3400..>3785
                     /locus_tag="LNE23_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK6932564.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DAFCNS010000136         3718 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFCNS010000136 DAFCNS010000000
VERSION     DAFCNS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.143"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            84..836
                     /locus_tag="LNE23_002757"
     CDS             84..836
                     /locus_tag="LNE23_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6932565.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            833..1747
                     /gene="pfkB"
                     /locus_tag="LNE23_002758"
     CDS             833..1747
                     /gene="pfkB"
                     /locus_tag="LNE23_002758"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK6932566.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1765..3696
                     /locus_tag="LNE23_002759"
     CDS             1765..3696
                     /locus_tag="LNE23_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6932567.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1197 a          663 c          850 g         1008 t
ORIGIN      
        1 taatttttat gaacgttttt gactgattta tattgctagt agaaaaaaaa tatgctatta
       61 tttgtattga ggtgatcgtg aaaatgctta cagaggaacg cagacaaaaa atcctagact
      121 tgcttgaaca gcagaaaatc gtgaaatcac aagatcttgt aaaccttttg aatgcttctg
      181 aatcaacgat aagacgtgat ttacaagagc tggagaatag tgggtttcta gaaagagttc
      241 atggcggagc taagaaagag caattattga attttgagca gaatatgagt gaaaaatcac
      301 tcaaaaacgt tcacgaaaaa cagcaaatcg ccaaatcggc agctgaaacc attaatgacg
      361 aagatgtcat ttatttagac gctggctcta ctacgttaga aatgattcct tttctgcaag
      421 gaaaacagat cacagtagtg acaaattccg ttcaacatgc ggcaaattta gttgatttgg
      481 agcttgctac cattatttta ggtggaacga taaagctggc aactaatgct gttttaggat
      541 ctaatgcgat agaacaacta aaaaattttc attttaataa agcatttatg ggaatgaacg
      601 gtgcccatct tgaacaagga ttcacgactc cggatccaga agaggcagct gttaaacgtt
      661 tagccatgaa aaattccgaa gagacatttg tattgattga tcataccaaa ttcaaccagt
      721 tgtccttcac ctctgttgca cctttggaag cagccacgat cataaccgag cgttgtccat
      781 tagaattcgt taatgaattc cgtgaaaaga caacaattaa ggaggcaata caatgattta
      841 cacagtaaca cttaatccat caattgatta tatcgtacgc gtagatgggt taaaactagg
      901 cgatttgaac cgaatgaagg aagacttcaa gttacctgga ggaaaaggga tcaatgtttc
      961 ccgtattttg aaacggatcg attctcaatc tacagcacta gggtttttag gagggttcac
     1021 tggcgaattt atttcgaagt ggcttgaaaa agaacaaatc cagtctttgt tcacaaccgt
     1081 aaaagaagat acaagaatca atatcaaact aaaatcagac actgaaactg agatcaatgg
     1141 cttaggccca tctatttcag atgaagaaat cgatgagttg aagcaagcat taggaacagt
     1201 aggacaagga gatatcgtcg tattgtcagg aagcgcgcca gctagcctgc gaaaaggatt
     1261 ttatcaagaa ctgattgata tcattcgaga aaaaggtgca gatttcgtga ttgatacaac
     1321 aggtgatgac ctaaaaaatg cacttgttaa gaaaccgttg ttgatcaaac caaacaatca
     1381 cgaattagct gagctttaca acacaacctt ccaatctgta gaagaaatca ttccgtatgg
     1441 gaaaaaatta ctagctgaag gggcacagaa tgtgctgatc tcaatggctg gagacggtgc
     1501 tttgctcttc acggaacaag gaacttataa aacgaatgta ttagttcgcc cattgaaaaa
     1561 ttcagtaggt gcaggggatt caatgattgc tggctttatt ggggcttaca gccgtacgaa
     1621 agatccaatc gaagccttta aatggggagt tgcttgcgga agcgcaacag ctttttctga
     1681 tgacttggca gctcgcgaat tgattgatga gttgctgcca gaagttgaaa tcacaaaaat
     1741 tgattagttt caaggaggat aatcatgaat atcaaggatt taatggtcaa agaagcgatg
     1801 atcatggatt tgcaagcgac tgataaaaaa ggcgcaatcg atgaaatggt ccaaaaaatg
     1861 tttgatgccg gccggatttc cgatatcgaa acatataaag aaggaattct agcccgcgaa
     1921 gcacaaactt ctacaggtct tggtgatgga atcgccatgc ctcacgcgaa aaacagtgca
     1981 gttaaagaag caacagtttt attcgcaaaa agccaaaaag gagtagatta tgaagcctta
     2041 gacggacagc caacttatct gtttttcatg atcgcagctc cagaaggcgc aaacgacaca
     2101 catttgcaag cattagcagc actatctcga ttgctgattg atgcagattt cgtaggaaaa
     2161 ttaaaagaag caaatacacc agaagaagtc caagacttgt tccaaacagc tgaagagcaa
     2221 aaagaagcag aagaaaaagc agaacaagaa acacaagctg cagaagcttc tgctcatcca
     2281 gaaagaaaat tcgttgttgc cgttacagct tgtccaacag ggatcgctca cacttacatg
     2341 gcagaagatg ctttgaagaa aaaagcgaaa gaaatgggtg tggagatcaa agtcgaaacg
     2401 aacggttcag aaggaatcaa aaaccgattg actgctgaag acatcgcccg tgcagaaggt
     2461 gtaatcgttg cagcagacaa aaaagtagag atgaatcgct ttgacggaaa agaactgatc
     2521 aatcgtcctg tcagtgatgg tatccgtaaa ccagaagagt tgatcaatct agcccttagt
     2581 ggtactgcac cagtattcca tggtgatgga aaagaaagca gttcagaaga aggaaatgcg
     2641 gatggtacta tcggacaaag aatttacaaa gatttgatga acggcgtatc acacatgtta
     2701 cctttcgtta ttggtggagg aatcgctatt gcgctttctt tcatgatcga ccaatttatt
     2761 ggcgtgccac atgatcaatt atcaaatcta gggaattaca atcaagctgc tagctggttc
     2821 aaccaaatcg gacaagctgc atttggtttc atgctgccgg tattagcagg atttattgct
     2881 tcaagtatcg gtgaccgccc tggtttgatc gttggatttg ctgctggtgc attagctaat
     2941 acaggcggtg caggattctt aggtgcactg attggtggat tcttagctgg ttatgtcatc
     3001 gtatttttac gtaaattatt caagaactta ccaaaatcat tggatggaat taaaacgatc
     3061 ctcttttatc cagtgtttgg cttactgatt actggtttct tgatgttgct ggtcaatgtc
     3121 cctatgaaag caatcaatga tgcattgaac ggtttcttga caagtatgag tggttcgaat
     3181 gctgctttac ttggtgcatt attagctgga atgatggcgg cagatttagg cggtccaatc
     3241 aacaaagccg cttatgtgtt tggtacagca actttagcta cgactgttgc aactggcgga
     3301 agtgtcgtga tggcttctgt tatggctgga ggaatggtac ctccattggc aattttcgtt
     3361 gctacacgtt tgtttaagaa taaattcagc aaaacagatc aagatgctgg tttgacgaac
     3421 atcgtgatgg gactatcgtt tgtcacggaa ggatcgattc catttgcagc agctgacccg
     3481 attcgtgcca ttccaagttt tattattggt tctgctttaa ctggcggatt agtcggagct
     3541 ttcggaatca aattactggc tcctcacggc gggattttcg ttgtattctt actgagtcac
     3601 ccggtcatgt atttggtgtt tattgctatc ggagcagtcg tatctggtgt gatttatggt
     3661 ttattgagaa aagctcctgc agtagcagaa gcatagaaat aaaaaagaag aggctggg
//
LOCUS       DAFCNS010000137         3600 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFCNS010000137 DAFCNS010000000
VERSION     DAFCNS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.145"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..185
                     /locus_tag="LNE23_002760"
     CDS             <1..185
                     /locus_tag="LNE23_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6932568.1"
                     /translation="EDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVY
                     EGHFVIAFKSGIEMEI"
     gene            328..552
                     /locus_tag="LNE23_002761"
     CDS             328..552
                     /locus_tag="LNE23_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6932569.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            567..1436
                     /locus_tag="LNE23_002762"
     CDS             567..1436
                     /locus_tag="LNE23_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBK6932570.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1417..2151
                     /locus_tag="LNE23_002763"
     CDS             1417..2151
                     /locus_tag="LNE23_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6932571.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2184..3092
                     /locus_tag="LNE23_002764"
     CDS             2184..3092
                     /locus_tag="LNE23_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBK6932572.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3089..3569
                     /locus_tag="LNE23_002765"
                     /pseudo
     CDS             3089..3569
                     /locus_tag="LNE23_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          788 g         1040 t
ORIGIN      
        1 tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa tgagctgatt gaatttatcc
       61 gcaagaacaa attccgcacc ttagaatatg atgataagct tgtaaggaag ataatccaga
      121 gcgttacagt ctatgaaggc cacttcgtca tagccttcaa atctggcatc gaaatggaaa
      181 tatgaaaacc agaagtaaat agcccatgac tctacagtag agttgtgggt tttcttttgc
      241 ttgctgatta taataatatt ttaataacta ttgtgtttat caaccaaatg gtttataata
      301 ttcttagcgt aaacttggag gtgctgtatg actacggcag aaatgattaa agaactgtgt
      361 gagcaaatga atataagtgt ttccgaactt gctagacgta ttggccagac tccacagaat
      421 ttcaataaaa aattacaacg agaaacagta accttggatg agttgaaggc cattgctgat
      481 gtgctaggtg tcaagtttgt gcaggcattt attttaccag atggagaaga aataaaaata
      541 tctaacgaat aaaggaggat gtttatatgg taggtaatgt tattcaaata gtaacagaga
      601 aattatcatc tttgcctttt atagaaggca tcgtattagg gggctcacgt gcaagaggca
      661 cccatacaga aaattctgat atagatatcg gaatctatta caattcagac tcatttgacc
      721 tgacagcgat taatcaaatt gctacagaat tggatgataa aaatagaaac aaccttgttg
      781 tgcctcccgg agcgtggggt gattggatta atggcggcgg atggttattt ataaatgggt
      841 atcatgttga cttgatttta cgtgatataa aacgggtaga acaaataatc aaagatacgg
      901 agcaaggaat tgttactgcc aattatcaga ccgggcatcc ccatggttat ataagtgcaa
      961 tgtatcgtgg agaattagcg attagtaaaa tactatatgc taagaacgaa agcttatgcg
     1021 aattaaaaaa gcaggcagaa atttacccta ctgctctaaa gaaaagcttg atgaactttt
     1081 ttatatttga agctgaattc tctttaatgt ttgtaaaagc aaatgcgggg gtagaggata
     1141 aatattatat tgcaggccat gtttttcgta ttatttcatg cttaaatcaa gtactatttg
     1201 catgtaataa tgcttattgc attaacgaaa agaaagctat aaaactgctt gaaacttttg
     1261 aatataaacc tgaaaaatat gccgaaagag ttaatcatat ttttgaagta ctcggtcttt
     1321 ctctttttga atgctacgat atgaccgaga aactttataa agaagtgaat gaaattgtat
     1381 cggagataaa taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat
     1441 aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg
     1501 ggagaatggg agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat
     1561 ttaggatgcg gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta
     1621 gtaggtgttg atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca
     1681 cagattgaat atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat
     1741 gtaatactaa gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag
     1801 atatatagga tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt
     1861 actgctcatg gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg
     1921 gtggacaatt attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca
     1981 aaatatcata gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata
     2041 aatcagattg tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat
     2101 gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga
     2161 aaaaataaac gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt
     2221 ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata
     2281 ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag
     2341 aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa
     2401 agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat
     2461 ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc
     2521 taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca
     2581 tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt
     2641 gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa
     2701 ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat
     2761 catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta
     2821 ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt
     2881 cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg
     2941 gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat
     3001 ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg
     3061 ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag ggcacctgaa
     3121 agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac cgaggtatga
     3181 aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa aaagctacca
     3241 agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg acaatactga
     3301 taagataata tatcttttat atagaagata tcgccgtatg taaggatttc agggggcaag
     3361 gcataggcag cgcgcttatc aatatatcta tagaatgggc aaagcataaa aacttgcatg
     3421 gactaatgct tgaaacccag gacaataacc ttatagcttg taaattctat cataattgtg
     3481 gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa
     3541 aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct
//
LOCUS       DAFCNS010000138         3519 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFCNS010000138 DAFCNS010000000
VERSION     DAFCNS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.146"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2232)
                     /locus_tag="LNE23_002766"
     CDS             complement(<1..2232)
                     /locus_tag="LNE23_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932573.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="LNE23_002767"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="LNE23_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          994 a          778 c          551 g         1196 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttt
//
LOCUS       DAFCNS010000139         3330 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFCNS010000139 DAFCNS010000000
VERSION     DAFCNS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.147"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..734)
                     /locus_tag="LNE23_002768"
     CDS             complement(<1..734)
                     /locus_tag="LNE23_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBK6932574.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYTINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSAMKYGIPLTLETPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWH"
     gene            complement(731..1150)
                     /locus_tag="LNE23_002769"
     CDS             complement(731..1150)
                     /locus_tag="LNE23_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK6932575.1"
                     /translation="MEKYLNTFSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVEVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(1220..1360)
                     /locus_tag="LNE23_002770"
     CDS             complement(1220..1360)
                     /locus_tag="LNE23_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK6932576.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(1353..1700)
                     /locus_tag="LNE23_002771"
     CDS             complement(1353..1700)
                     /locus_tag="LNE23_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932577.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            complement(1714..>3330)
                     /locus_tag="LNE23_002772"
     CDS             complement(1714..>3330)
                     /locus_tag="LNE23_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932578.1"
                     /translation="TQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEI
                     KGRMDSLNKQITDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPN
                     LMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKV
                     RFDEGTTGAIDKLRLVYRTSPGEKILLEANNTTITIDDVGKEITIKGTANVNYQITNL
                     DRFYISVSFSNRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANK
                     GTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPV
                     EGLTDVWSLTFTPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYK
                     YRGIGMRDSNNPKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDTTTLTVTNGNTG
                     SAKLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSA
                     QLSIGADGAIKWRRNSSYGSAYSFVITYSI"
BASE COUNT          898 a          686 c          551 g         1195 t
ORIGIN      
        1 atgtgccatc cagcgacgtg taagttcgca ttttcgattt ctccaaactg atcaatgtgg
       61 gcgtaatttg ttccatctgt cagtgtagga tttgctgcac ctgctctagt tggatcaatg
      121 actggtttat catctgaagt tgtcggattt tcatcggtaa atccatgagc taaatcatat
      181 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      241 ccccagatat tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggcgtt
      301 tctaatgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      361 ttgtagttct tttcaaacgt tactttatcg tgcgtatgtt gcagttcgat ttgcacagga
      421 ctatttgcat tagcatatga accagcgcca tattgtacat aaccaggttg tccaacttgg
      481 taaacgatcc caccatcgcc aacaatatac gctgtatagg cattgtacca agaacgtttc
      541 atgtactgcg cttcatttcg tcctgtcgca gtttcgtttg ctgtttcatg caataggatg
      601 tattggttat ttgctacttg agaactacct tcgtttgcgc ccaaattgaa ttcattgttg
      661 atcgtgtaag caaaggcgtt tattggcaat aaaaaaagag ctataatagc tcctacaaag
      721 attttctttt tcatttggtt cctcctcggt ctttattttc agtatcattt cgtatttgtt
      781 ggaatacctc ttttaactta tctggcaaag gaaggaatac cgaaatgttt tctaaaaaac
      841 taatcccttc atttgcaaca tagaacataa taactatttc tcttagaggt atagaattcc
      901 caacgatttt ctgtacttca accgcaacgg caatcacaac gaagattaag actttcttca
      961 acaatccttc gaaacctatc tcactactta tttctttcaa tttccatgcc ttagcaagtc
     1021 cagttaagaa gtccacaccc acaagaaaaa ttaaaacatg tagtatattg tccatccctc
     1081 ccaataaacc aacaattgtc ccgcctatca cccctgtcat tactgagaat gtatttaaat
     1141 atttttccaa tatctatttc tcctttatgc aaaaaagcgc actcgtttga gtacgctttt
     1201 tatttaactt tgtgcttaat taaatttcat ttccagtaat ctgcttatat tggtcttttg
     1261 ttaaacagtt catgtttaca tagtcaagta aatcttggtc cgtataacat ccccaatcat
     1321 aaaattgttt tatattttca aagcccggaa aactattcat tacttttccc tccattctta
     1381 atacttgcga cttccatcgt caattgagct aacatttgtt gcattttttc ttgttcctgt
     1441 accgatatcg ccaattgttt tgtcagtaga gcatttaatt gcttcaactg atcgacatct
     1501 tcaatatcgt caaccgctgt gccactcttc tcttcttcag tagctaactc aacccactgt
     1561 tttttatcaa aatcaaatct tggtctccag ttaggaactg gtggtttaat ctctgtacag
     1621 ttttcaggga tattttcttg attgttcaaa atgatttgct cgaatccgta aggcttgatt
     1681 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1741 ataagctgat ccataacttg aatttcttct ccatttaatg gctccgtctg cgccaataga
     1801 tagttgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1861 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1921 tccgtttaaa gccacaaaat atatagaaac tgtttttcct tcacgataaa gctttgctga
     1981 tccggtattc ccgtttgtaa ctgttaatgt ggtagtatcg gtataattgt ttgctttacc
     2041 tgtgatttca gcaaccttga tatctgtagc taggttattc tcaacatagc taggttctag
     2101 atcccataca taatcttttg gattgtttga gtctcgcatg ccgattcctc ggtatttata
     2161 ttgctcaata ttcggggttc gtgtgtcgcc tttttcgatc ttgagccagt caattcggca
     2221 tgcgccggcc gtttctttag gcgactgaaa aatgcgaagg tctttaggca aacccggttc
     2281 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     2341 atctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     2401 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     2461 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctt
     2521 acccaacgat gttttaccta cccagtaagg gtcatctagt aagtttggct gatacggagt
     2581 ggctgttgag ccttcttcga ttttgatgtc gtacaattta aatccaccat ttattttatc
     2641 cctgttagaa aaactaacac taatataaaa acgatctaaa tttgtgattt gataattaac
     2701 gttagctgtg cctttgattg ttatttcttt ccctacatca tctattgtaa tagttgtatt
     2761 atttgcttcc aataatatct tttctcctgg tgatgtacga tacaccaaac gtaatttatc
     2821 aatagctcct gtagttcctt catcaaatcg aactttcgca ctcagagtat aggttttccc
     2881 actagcaagc tgaggtgtat taatacgcgt aaacataata atgcttcctg taccatcaga
     2941 agtaaaatgt agcttttcgt tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc
     3001 gctcgatttt agtttggaca ttaaattcgg attcccacta taatcatagc ccccgaagtc
     3061 gatgctgttg gagtacatca catgtaagtt acctaattta gaaatttctt ctttcagagc
     3121 atctaacttg tcttgtagtg ttttagcttg accagttaaa tcagtaatct gtttatttaa
     3181 gctatccatt ctacctttaa tttcagccag aaaagcatca aaagtttcgt tatactttcg
     3241 aatcaattct tctaattgcg aaacatattc atcggcttgg ccttgcgaaa tgtcagacac
     3301 tcctagtgag aaaaaaatga tatcttgcgt
//
LOCUS       DAFCNS010000140         3222 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFCNS010000140 DAFCNS010000000
VERSION     DAFCNS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.148"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(442..1056)
                     /locus_tag="LNE23_002773"
     CDS             complement(442..1056)
                     /locus_tag="LNE23_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6932579.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1662..2039)
                     /locus_tag="LNE23_002774"
     CDS             complement(1662..2039)
                     /locus_tag="LNE23_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932580.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2339..3220)
                     /gene="galU"
                     /locus_tag="LNE23_002775"
     CDS             complement(2339..3220)
                     /gene="galU"
                     /locus_tag="LNE23_002775"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK6932581.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          979 a          659 c          498 g         1086 t
ORIGIN      
        1 atttttgtgt attttatttc tattagaaaa aggtatcctg ttggacaatt ttgttatatc
       61 aggtaaactt cattgcaata atgataacaa atgtactata attactttgt aaagagtttt
      121 ggggaagact ctttcatcaa cgtgtattaa gagacaagaa agaatccata tggattcttt
      181 cttgtctctt tttccactac tttttatttt tctgacacta catattctta taactcattt
      241 ctcataactg tgaaacaact tataaaaata acaaacttta aacaaaacaa gcttaaaatc
      301 aaagatgatc catcttaaaa taactaaaac aaaaaaagta gtcgttgtta atcctcatta
      361 ataatcgttt tctttttttc aaacgctcca tttttatgtg gaacattatt tttttaagga
      421 actttgtcaa catgtggata actattttcc acttgtgtcc tggactagct gttttgctgt
      481 aaaatacaaa acttgctgca gcaaaaagat cgttgcaaga gaggctgttc cgcagatcgc
      541 tggatctaga tttggcaaag tcaagactgc atcatgtaaa ttggcgtggt tgtccaaaac
      601 caaatcagca aattcaaata atcgcaaacc actgctatgt tgcgatttga tacttcgtga
      661 caaatcaaat ccagtaacaa tgattaatgg atgaccattt tctttgatcc actgagctag
      721 ttcgatgatt gccgggtttc ttccttcatt cgaaagaaga agaaaaatgt cttctgattt
      781 gacagaaagc tgatcaataa ttgcctgtcc tgcaccttct agcgtttcat agacacctcc
      841 ggctggattt tcaatcaata tacttggtac aagatccggg gtttgatccc aaaattcaaa
      901 tgctacacca cttagtgtgc ggctagcata tatctgcaat ctaccacctt gattaatttg
      961 tttcgcgatc caagtgccag cttgaagcat cagcggctct tctatcctta atttctcttc
     1021 caattggtga taatagtcaa aataacataa actcatttat ctctcccgcc tagctatact
     1081 tgacatgaat agaaacagct gcctaatcta aggaagtaca taaaaaaaca gtagttagac
     1141 agtaacacat gtggtctttg ctgtttcaaa cacaaaaaag gcattgaacg aatacgccca
     1201 ataccttgac atagcaaata aaacaacgtg cagaccatgg aaaatatcta cataggggta
     1261 gaattttcac cctagattag gtacaataaa ttaggtcgat gcactgcatt gactatggca
     1321 aaatgatcgg tcagggtgta tgtgtgtgac tggtcattcc ctgattctta gcgccaacta
     1381 agggtcaggg agcctttttt tattcagctg tttctatctg aatttattgt aagtaatctc
     1441 ttatccaagt gcaagcaaga aaatacaaaa aaacattggt atgatttata tatatataaa
     1501 taacataaaa aaaggaatgg cttatcccga tacaacaagc attcctttaa aaaagattct
     1561 tttatataag tgaaaaaagc ttttttccac caattaaata aatctctcat ctcaatagtt
     1621 tatccattat tttatctttt tttgcgtata cctactaaat attaccgtct tcttctcgtt
     1681 cttctacttt gttcgagaga atctgtacta gaaaaagtag ttgtatgtct accttttctg
     1741 cctatccgag actttctttt catatatggg atataaatga ttagatacag tacagcaaaa
     1801 aataaagcaa atacgcaagc ttcccaacta cctaacgcca attcaaccat caaggtagca
     1861 accccaacgg atgctgcata ccagataaaa atcagaaacg tttctttttt tctgaccatg
     1921 cgaaacatga taaatagact aataagcaat agtggcaaca caaatatcca attgcttcct
     1981 tccatcagcc acttgagtat atcaagcagc gactgtgcaa cagcgactaa ttttcccata
     2041 gtgaacacac accttttttt ataattaaaa aattcgcctt tttattctac tatgaatctt
     2101 ctggcttggc tttcatttct cttatgtaat cattttcttt tatacaactt aaaaaaaaat
     2161 taaaccatta gcataatatt ttctgaatct attcacgaaa gtctttttct atctttcgtt
     2221 ttccacgact caactgatct agagtcaaaa aactaaaacg acagttgcgc aaatgacgca
     2281 caagctgccg ctttaataac aaatagcctt tcattctcag caggccactt atttccagtt
     2341 aactttctgt tcttagcttt ttactcaact ggataatata tgcacttaat gaagtcccaa
     2401 tttccgaatg ttgaagtcca tattcaatat ttgtcttcat atatcctaac ttatcgccta
     2461 cgtcgtatct ttttccttta aactctctcg catagaccgc ctctgtctgg ttaagagtct
     2521 gaagtgcatc cgttagctgt atctcgtttc ctgctcctgg ttcttgagtt tctaatatct
     2581 caaatatttt aggagtcagt agataacgtc cgataatggc taaattactt ggtgcttttc
     2641 ccatttcagg tttttctaca aaccctttta ctctgcaacg acccttttcg ctatctgttt
     2701 taggatcaat aattccgtat ttagatgttt cattttctgg tacacgcatg acggcaacag
     2761 cggaagcttg tgtttcttca taatccatca tcaattgttg agtcaatggg atatcatctt
     2821 tcatcaaatc atcccccagc atgacgacaa acggttcatt tcctataaat gcttttgctt
     2881 gaagcacggc atcccccagt cctcttggat gagattgacg gatgaagtga agattgactt
     2941 gagtcgtttc ttcaactaaa gcgagtaatt ctgttttttc cttttcccgc aaattgtttt
     3001 ccaattcgat atttgcatca aagtgatctt caatgggtct tttttctttt cccgttacga
     3061 tcaagatgtc ttcgattccc gaatccaatg cttcttccac aataaactgg atcgttggtt
     3121 tatccacgat tggaagcatt tcttttgcca tggcttttgt tgccggcaaa aaacgtgttc
     3181 ctagcccagc ggcaggaatg actgcttttt tgaccttcac tt
//
LOCUS       DAFCNS010000141         3199 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFCNS010000141 DAFCNS010000000
VERSION     DAFCNS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.149"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            301..585
                     /locus_tag="LNE23_002776"
     CDS             301..585
                     /locus_tag="LNE23_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932582.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="LNE23_002777"
     CDS             588..1004
                     /locus_tag="LNE23_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932583.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="LNE23_002778"
     CDS             complement(1141..1860)
                     /locus_tag="LNE23_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6932584.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="LNE23_002779"
     CDS             2513..2608
                     /locus_tag="LNE23_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBK6932585.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="LNE23_002780"
     CDS             complement(2910..3155)
                     /locus_tag="LNE23_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932586.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAFCNS010000142         3071 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFCNS010000142 DAFCNS010000000
VERSION     DAFCNS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.150"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            287..1102
                     /locus_tag="LNE23_002781"
     CDS             287..1102
                     /locus_tag="LNE23_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932587.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1228..1485
                     /locus_tag="LNE23_002782"
     CDS             1228..1485
                     /locus_tag="LNE23_002782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932588.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1737..2309)
                     /locus_tag="LNE23_002783"
     CDS             complement(1737..2309)
                     /locus_tag="LNE23_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6932589.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2325..2930)
                     /locus_tag="LNE23_002784"
     CDS             complement(2325..2930)
                     /locus_tag="LNE23_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBK6932590.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1018 a          539 c          473 g         1041 t
ORIGIN      
        1 aaataagaaa ttcaaaaaag aaaaaacttt ccttaaaaac aaaaaatagg agtattcagt
       61 ttcttaatag ttttgtaata gttgtatgta gaagttttct taatagtttt tgtaataatt
      121 tattaatcgt agtttatata acttatttgt aagtgactag agaattaaat aaaaaataat
      181 tactatctag tacgaaatta attacatagg ataatatatt aaggtgctgg actacaagta
      241 aaaaatactg tatgatcatg acagattcaa gaaaggagca ataagattgg ttaagatagc
      301 aggactttct aaaggtggaa aaaccgtttc acaactttat aaacaaaact acagagactg
      361 gagacagttc agaaaaaaca ttaaggattc ttactttatt ctaccaacag aacttgaaaa
      421 atatttaccg aataacaaag ctattaattt atatctgtat tactgtttta aggcaaagaa
      481 tgaaggcggg gattcctggc attcggtcaa taccatagca gagtctctag gagtgacaac
      541 taaaactata aacacctgga ataaaacctt agttgattta ggtatgattg cacgtataga
      601 cgataaccat ctcagtaaat cgacaatttt acttcctctc tctagttact ataaattagc
      661 ccttgatagt tctttaaagg atgttaccga gagcactgtc catgaaattg atggcaactt
      721 ggttgcagtt tatcatttgt ttgaatggag aaaaaatgag gctgatgaaa attataacgt
      781 tccttataac acactctgtt gtgtttttaa aagaaaaaca caaaaagata ctatctataa
      841 atttatcttg gtccaagatg agaaaataag taatttcgaa ttaagcactc cttctagtaa
      901 gatttatgac gatgcatata gatttgaatg tcaagatcta gaagccctcc tttccgcaaa
      961 taatctaaac aatatagaac atgaaaactt tgttgtaaac actagattta atttagtttc
     1021 tgaaaaggac tcagatttgt tagatttgct gatagagctt actaaaaatc tagataagaa
     1081 agaaaacatt actgtactat aactagcatc ggtccatcat taagtttgga ctattagaac
     1141 acttgctatt acagaaaaag gtatgctagc cacgcttact atttattggt atcaaacaag
     1201 tatcttaatt tgaaaggaag atgatttatg gctaatttat atatagagaa caaaggagac
     1261 ttgaatgcca ttattacttt aatcgtagtt atggtaaaat caataggtaa tatcacaata
     1321 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
     1381 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
     1441 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
     1501 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
     1561 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
     1621 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
     1681 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1741 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1801 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1861 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1921 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1981 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     2041 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     2101 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     2161 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     2221 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     2281 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     2341 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     2401 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     2461 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     2521 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     2581 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     2641 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     2701 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2761 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2821 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2881 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2941 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     3001 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     3061 gcacagtcta g
//
LOCUS       DAFCNS010000143         3031 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFCNS010000143 DAFCNS010000000
VERSION     DAFCNS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.151"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            581..1540
                     /locus_tag="LNE23_002785"
     CDS             581..1540
                     /locus_tag="LNE23_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6932591.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1527..2132)
                     /locus_tag="LNE23_002786"
     CDS             complement(1527..2132)
                     /locus_tag="LNE23_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932592.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2372..>3031)
                     /locus_tag="LNE23_002787"
     CDS             complement(2372..>3031)
                     /locus_tag="LNE23_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6932593.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          988 a          504 c          471 g         1068 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttaccttcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc
      541 aaaatagttc cgtcgaaaga attataagga gtgagtacaa atgacctata cccatcttac
      601 accaactgaa ctcgtaatga tagaagctta tttcaatcaa tcacactctg tttcaaaagt
      661 agcccatctg gtacaacgtt ctcgacaaac catttataat gtctatcgtt ttcttaaatc
      721 tggtggaagc gcaataagct actatgaaca atataagaaa aacaaacgta actgcggaag
      781 acgtccgatt gtgttaccag atgggcaaca agaatacatt caaaaaaagg tggctcaagg
      841 gtggactcca gatgtcctta ttggtcgagc agagtttcct attgcttgtt cggtacgtac
      901 actatatcgg atgtttaaag ataaaacctt tgaccctcac gatttaccaa tgaaagggaa
      961 aagaaaacca aacggacata aagaaaaaag aggaaaacaa gcttttcgcc gttcgattca
     1021 ggatagaaca actgattata tacagttcaa tcatgagttt ggtcacctag aaggtgacac
     1081 cattgtcggt ggaaaacata aaagtgcggt gatcacgtta gttgagcgat tatctaaagt
     1141 gatcatcacc ttgaaacccg ctggtaggca agctatagat attgagaata gactgaatca
     1201 atggtttcaa tctgtaccga aacacttgtt taaatccatc acatttgatt gtggaaaaga
     1261 gttctcgaat tggaaaaaga ttagtaatac caatgatatt tcgatttatt tcgctgatcc
     1321 aggaacacct tctcaacgag gactaaatga acattcgaat ggcttattac gtaaagatgg
     1381 cttatataaa gctatggatt ttaataaggt atccgaaacc tttatccaat cggtggcatc
     1441 aaaacgaaat catattccta gaaaatcatt acactatcgc acaccattgg aagtattttt
     1501 gagttacgta ggtaaagatg atttgtctag cttaatttga caaacgaaac tttgtaaaaa
     1561 tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat gtcaatattt
     1621 ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa ttccctgtaa
     1681 ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc acccattcca
     1741 ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc agtttgttta
     1801 tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc aaaaattttt
     1861 tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac tgataattga
     1921 tactctatat tacatgactt aattagtttt ttagatcccc cctccagata ggcatatgtt
     1981 tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc ataaattaaa
     2041 aatgactgtc tatttagccc ataatctaat acaacttctt catactcata tttctttaaa
     2101 tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt gttatttatt
     2161 gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt agtgaactac
     2221 tcaccactta gctaaaccta acggtttctt acgtttgaag tgggagcttc ttgggaagag
     2281 agcatacttg atagcgtatt actttactaa cagaggtgcc tcttatttac caagctatcc
     2341 ccgtagttcc tacggttctt gtttatttga tctaagccaa tcccatagag tgtagcctta
     2401 gaccttcggt caggatattg atactagcgt tgatatctcg gtcatgatga gtatgacaaa
     2461 tagggcaagt ccattttcga atttcgagag atttcttgcc gtctttatgt ccacaattcg
     2521 aacagatttg gctagatgga aaccacttat ctactttgat gattttccga ccgtaccagt
     2581 cagccttgta ttgtaattta gacacaaaac tagaccaaga aacatccgaa atactttttg
     2641 ctagtttatg attacgcaac atgccttttg tgtttaagtc ttcaatacag atgatatcgt
     2701 gatttttgat aatttctgta ctcaacttat tcagaaaatc agtacgttga ttcattactt
     2761 tttcatgcaa tcgtgctact ttacgttttt gtgtttgata atttctagct tcaaacagat
     2821 tgataccttt ttgtttagct aataaggcac gtcttgacaa cttacgttgt tcacgtttga
     2881 gtttcttttc cattttggac gtaaatttat tattatcaaa tttttgaccg gtagaaagaa
     2941 ttgcaaagtc tataatacct aggtcaatac caacagcaga attagattta ggtagttcgc
     3001 gaacttcttc tttgcataac aaagaaatat a
//
LOCUS       DAFCNS010000144         2952 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFCNS010000144 DAFCNS010000000
VERSION     DAFCNS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.152"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            89..406
                     /locus_tag="LNE23_002788"
     CDS             89..406
                     /locus_tag="LNE23_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBK6932594.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="LNE23_002789"
     CDS             complement(497..862)
                     /locus_tag="LNE23_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932595.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="LNE23_002790"
     CDS             1070..1552
                     /locus_tag="LNE23_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932596.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /locus_tag="LNE23_002791"
     CDS             1749..2639
                     /locus_tag="LNE23_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBK6932597.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1008 a          460 c          566 g          918 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2881 aaacttagac cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     2941 cagaccttgt tg
//
LOCUS       DAFCNS010000145         2939 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFCNS010000145 DAFCNS010000000
VERSION     DAFCNS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.153"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..236
                     /locus_tag="LNE23_002792"
     CDS             <1..236
                     /locus_tag="LNE23_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932598.1"
                     /translation="VGKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMA
                     TASYIYQLMMFKQARDAYQKEFEKANKEKKLQK"
     gene            438..>2939
                     /locus_tag="LNE23_002793"
     CDS             438..>2939
                     /locus_tag="LNE23_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK6932599.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWT"
BASE COUNT         1026 a          434 c          664 g          815 t
ORIGIN      
        1 ctgtgggaaa aggatccatg gatatttctg ttattcaagg tgcaaaggct gtgtatattg
       61 cttaccgcca agcgaacatg actgattata tttcattcga tgaatttatc gataaatggg
      121 attttgatat ggctactgcc agctatattt atcaattgat gatgttcaaa caagcacgcg
      181 atgcttatca aaaagaattt gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat
      241 tccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg acgttttttc
      301 tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc aaatggctta
      361 caacaagagt gcttatgaag gatggaaaaa ctatgcaata aaccaatccc agaaaaacta
      421 aagaaaggag gtaaaaaatg gctaaaaaga gaacagaagc agaagtaact ttcatagcta
      481 acgatgacgg attgaaatct acgttaaaag aaatcagtgc tgaattaact aaaaatagag
      541 cagaattaaa actagaacaa gctcaattac aacagactgg ttctgaatca gacaagttag
      601 gaagtaaatt atcttcttta gaaaagcagt atgaattaca aagtcaaaaa gttgaagtaa
      661 ctagccaacg tttagccaat gcgaaaaaat attatggaga aaattccacc gaagttcaga
      721 aacttgaaag agaactgatt aatcaacaaa cagcgcaaca acgtttgtca aacgaaattg
      781 ataaaacgag taatgcacta gctcaagcaa aaggcgaaat acagacgtac gagtctacaa
      841 tgcaacagtt ggatagcgaa caaaaaaatg ttcaagctag tgcttctctg attgaatccg
      901 aatacaaaaa atggcaagca actgctggtc aatcagcttc tgaagccgag aaattagcga
      961 aagcccaaga atatgtttct caacaatctg aaaatgcgga gaaaacgata gatatcctaa
     1021 gacgacagtt agaagctaca cagtctgaat ttggcgctac atccacagaa gcaatgcaga
     1081 tggaagcgaa gcttaatgat gctgaacgtg aatttgaaga gttaggacaa gctgctaaaa
     1141 atgtagatac aactaacttg gacgatatcg gaagcaaaat agatatgaat aatttaatgg
     1201 aagcttctga cgttttaagc gacattggcg ataagcttac agaattaggg aaacaagcag
     1261 tggactctgc taacagtgta ggtagttccc agagtaagat acaagctaat tttggtttgt
     1321 ctaaacaaga ggctgaagaa ttaacgaatg tagccagaga catttattat aaaggttttg
     1381 gagaatcgtt agatcagtcc acagatgcat tgattttggt aaagcgtaat ttaggagatt
     1441 taaataatca agatttacaa aatatcacgg aacaagctat ggtcctagaa aacaccatgg
     1501 gcgctgatat ggatgaaacg ttacgtggtg taaatggctt aatggtcaat ttcggcttga
     1561 gtgctcaaga tgcaatggat ttaatggttt cgggtactca aaacggttta gataaaacgc
     1621 acgaattagg cgacaatatg gcagaatata gccaattatg gagtcaaatg ggatattcag
     1681 ctgatgaaac gttcggaatg cttcaaaatg gtttagatgc gggtgcttat aaccttgata
     1741 aagtcaatga cttagttaag gaaatgggaa tatcgttaac agatggtcga tttgagcaaa
     1801 acatggatat gtttagtgaa agtactagaa aagcttttga agagtggaaa aatggcggag
     1861 gaacacaaaa agacgttatt aattccatga ttcaagattt tagcaatatg gatggtcaat
     1921 acgaccaatt aaataaagct tcgacaattt ggtctgcgct tggcgaagat aatgcgatga
     1981 aagttgtcca atctttaact gatgttaacc atacatttga tgatgttagt ggatctgcac
     2041 aaaaaatgaa tgaagattct actactccgt tgcaagagtt gaacgggaaa atagctgaat
     2101 taaaggattc attagctcct ataggcaaca caatcataga tgcactcgaa ccagtaattg
     2161 attttctagg aaagatggct gatgcgttta ataatcttcc acaaccagta caggatttca
     2221 ttgttgctat aggtggtctt acagcagcat ttggcatact gttaccggca atactagccg
     2281 tgtcatattt atttggtccg atgatgctta taattggagg aattatagct gttatagcag
     2341 gcgttattgt ggcaattaag aactggggtg caattactga ctggtttagt ggtttatgga
     2401 aaaaatttac tgattggttg ggtgatactt gggaaagtat aaaagacgga gcctcatcag
     2461 tttgggatgg ggttaaagaa acctggtctg gatttgtaga ttgggttcaa gatatttggc
     2521 aaggagtttc tgattggttt ggagagctat ggagcggatt agttgaagga gcttccaaca
     2581 tctggcaagg agtccaagaa acttggcaaa cattcgttga ttgggtttca aatatttgga
     2641 acggagtcaa agaagtatgg tcgattattt gggcagacat tgtaggaatt gttcaaatac
     2701 catggacatt aataacgtca ttgattcaag ctggtattaa tattatcgtg ggtatttttg
     2761 atgtagctgg acagttattg ggcgcagctt ggcaagctgt ttggacacct atttctgatt
     2821 tccttaaaaa tacttgggat actatgacac aatggataag tatcgcttgg aatgggattg
     2881 taactacatt ccatactata tttgatccag tagtggcatg gtggaatggt atatggaca
//
LOCUS       DAFCNS010000146         2938 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFCNS010000146 DAFCNS010000000
VERSION     DAFCNS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.154"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            9..731
                     /locus_tag="LNE23_002794"
     CDS             9..731
                     /locus_tag="LNE23_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6932600.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            789..2828
                     /locus_tag="LNE23_002795"
     CDS             789..2828
                     /locus_tag="LNE23_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBK6932601.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGATVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGFDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
BASE COUNT         1040 a          518 c          627 g          753 t
ORIGIN      
        1 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
       61 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
      121 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
      181 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      241 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      301 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      361 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      421 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      481 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      541 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      601 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      661 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      721 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      781 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      841 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
      901 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
      961 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1021 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1081 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1141 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1201 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1261 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1321 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1381 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1441 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1501 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1561 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1621 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1681 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1741 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1801 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1861 gtctaggagg aaaagctgga gcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     1921 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     1981 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2041 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtttcgac aacggcacta
     2101 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2161 gatcttttca aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2221 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2281 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2341 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2401 gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg tattctgttt
     2461 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2521 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2581 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2641 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2701 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2761 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2821 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     2881 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactc
//
LOCUS       DAFCNS010000147         2841 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFCNS010000147 DAFCNS010000000
VERSION     DAFCNS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.155"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..236
                     /locus_tag="LNE23_002796"
     CDS             <1..236
                     /locus_tag="LNE23_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932602.1"
                     /translation="MGKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDME
                     VAVAVYSTMMFKQARDAYQKEFEKANKEKKLQK"
     gene            239..421
                     /locus_tag="LNE23_002797"
     CDS             239..421
                     /locus_tag="LNE23_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932603.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            439..>2841
                     /locus_tag="LNE23_002798"
     CDS             439..>2841
                     /locus_tag="LNE23_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6932604.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWT"
BASE COUNT         1012 a          417 c          650 g          762 t
ORIGIN      
        1 cgatgggaaa aggctctatg gatattacag ttattcaagg tgcaaaagcc gtatatgtag
       61 cttatcgtca agcgaacatg gtcgattaca tttcattcga tgaatttata gataaatggg
      121 attttgatat ggaggttgca gtagctgtat acagtactat gatgttcaaa caagcacgtg
      181 atgcctatca aaaagaattc gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat
      241 gccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg acgttttttc
      301 tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc aaatggctta
      361 caacaagagc gcttatgaag gatggaagaa ctatgcaata aatcaatccc agaaaaacta
      421 aaggaaagga ggtaaaaaat ggctaaaaag agaacggaag cagaagtaac tttcatagct
      481 aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg ctgaattaac taaaaataga
      541 gcagaattaa aactagaaca agctcaatta caacagactg gttctgaatc agacaagtta
      601 ggaagtaaat tatcttcttt agaaaagcag tatgaattac aaagtcaaaa agttgaagta
      661 actagtcaac gtttagccaa tgccaaaaaa tattatggag aaaattccac cgaagttcag
      721 aaacttgaaa gagaactgat taatcaacaa acagctcagc aaaggttatc taatgatata
      781 gataaaacga gtaaggcatt ggctcaatct aaaggtgaat ttaaaacgta tgagtcaact
      841 atgaaagact tagataatga gcaacaacat ctaaagtcaa gcgcatcatt agtagaatcc
      901 gaatataaaa aatggcaagc aactgctggc cagtctgcta cagaatctga aaaattagca
      961 aaagcacaag aatatgtcgg caaacagagt gatattgctg aacaaaaaat cgaagtgcta
     1021 aaaaaacaat tagatgccac acaaaaagaa tttggagaaa catctaccga agctttagaa
     1081 atgaaaacta agcttaatga tgcggaaaga gaattcgaag aattaagtaa tgctgctaaa
     1141 aacgttgata cctccacagt agatgatatc ggcaaaaaac ttgatatggg taatttaatg
     1201 gaagctactg atcacttgtc agtgatcgga gataagctta ttgatgtagg tagtaagtct
     1261 attgaagcag ctggaaaagc acaagctatg caggcccaat ttaaacaagt ctttggctct
     1321 ttagaagggg aagcacagga cgccgttgag ggaatggctg aagaatttgg aatgttacca
     1381 aatacgatca agcctgtttt tatacaatat acgtcaatgt ttaaaggact tggatatgat
     1441 accaaagaag ctatggagtt agctggtgat agtactcagt tagcagcaga tgcagcagct
     1501 ttttacgata agtctatgga tgatgctagt gaatctctta attcatttat aaaagggaac
     1561 tacgaaggtg gggagcaaat aggtttattt gctaatgata ctcaaatggc agcttatgct
     1621 gttaagcata atttgatacc agcgactgaa ggagcaaaaa aagccagtga agagtcattg
     1681 ttagctgttg aaaaagcaca atctaagtat gctgatgcta ttaagaaaca tggtgaagga
     1741 tctttagaag caagagaggc tgctttaaaa cttaaagatg cgcaagataa aataaatgaa
     1801 gaattaggcc cacaaacgca aaaatggtct gatttagatg aggctaccaa gcaagcagtt
     1861 cgagttcaat atgctgaaga tatgcaaaaa ttagccggtg ctacaggtca ggctagtagg
     1921 gagtctgatg gtttagaaaa ccaaatgaca agagcaaagc aagcgttaga agatttttat
     1981 gcctcattgg gtgaagatat actgcctgtg tttatcaaag ggttgcaagc aggagcgaaa
     2041 gctttgcaag gattagccga atggtggagt aaacttgatg ggccaatgaa aaatttcatt
     2101 ttagctcttg gaggaattct agcattatta agcacattag ctcctgttat aaccgcagtt
     2161 gttacgatag ttggcacatt tggttctaca gttttgctgc caataatagg aattatagcg
     2221 ggcgttgcag ctgtaatagc gattgtaatt acagcgtttc aaaactgggg cgcaatcact
     2281 gactggttta gtgatttatg gaaaaaattt accgattggt taggtgatac ctgggaaagt
     2341 ataaaagaaa gtgcctcatc agtttgggat ggagtcaaag aaacctggtc tggatttgta
     2401 gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt atggagcgga
     2461 ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca agcattcgtt
     2521 gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat ttgggcagac
     2581 attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca agctggtatt
     2641 aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc ttggcaagct
     2701 gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac acaatggata
     2761 agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc agtagtggca
     2821 tggtggaatg gtatatggac a
//
LOCUS       DAFCNS010000148         2736 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFCNS010000148 DAFCNS010000000
VERSION     DAFCNS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.157"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            78..524
                     /locus_tag="LNE23_002799"
     CDS             78..524
                     /locus_tag="LNE23_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK6932605.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            797..2149
                     /locus_tag="LNE23_002800"
     CDS             797..2149
                     /locus_tag="LNE23_002800"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBK6932606.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2320..2607
                     /locus_tag="LNE23_002801"
     CDS             2320..2607
                     /locus_tag="LNE23_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932607.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          960 a          449 c          544 g          783 t
ORIGIN      
        1 ttttcttttc atgttaagtg gattttaaaa tgtaaaaata gtatgataag acgagaataa
       61 caacgtgggg tgtttacatg gaaaataagt tagtcgaaga ggttatcgaa aagttaaaaa
      121 aagagaacat tcgaatcaca cctcaacggt acgcggtttt agaatatttg attgaacatc
      181 attcccatcc aacggctgat gagatttata aagcacttga acatcggttt ccaaatatga
      241 gtgttgcgac tgtttacaat aaccttcgct tatttacttc gattggattt gttcaagaaa
      301 tgaagtacgg cgatgcttct agccgatttg atttcagttc aaagcgtcat tatcatgtaa
      361 tttgtgaaaa ctgcggtaag attgttgatt ttcattaccc agggcttgaa gatatagaaa
      421 tggctgcaag cagactgact gattttgaga ttaatgaaca tcggttggaa ttgtatggac
      481 tctgtccgga ttgtaaagag tcagcaaaaa aggcgcaaat gtaaaaacag tttctacaag
      541 tgttatacct aaaaaaagct agctgtgtaa aaaaaacgct agctttttta tttttttgtc
      601 ttttattcac aatcttagat ataaaagtga atattattaa aataagaatc ttgtagatat
      661 tgggttctta tgcttgaaaa ggaatgaaac tacaaacaaa cctttgtgaa ttcacaagca
      721 ttaaactgtt gtatccgttt tatttctgtt atagtagttt tgtcgaagga caaaatgtta
      781 atcggaggga ccataaatga aagtagtagt aattggctgt actcatgcag gtactgcagc
      841 tgtaaaaagt attttaaaaa atcatccaga agcagaagtg acagtatatg aaagaaatga
      901 caatatttct ttcttatctt gcggtatcgc actttatgtc ggcggagtag taaaggaccc
      961 agcaggcttg ttctactcaa atcctgaaga attatcttca ttaggagcaa cagttaaaat
     1021 ggaacacgat gtaacagacg tcgatacaga aaataaaaca gtaacggcaa aagatctgaa
     1081 aacaggagaa acagaaacgg tttcttatga taaattagtg atgacaactg gttcatggcc
     1141 tattatccca ccgattagtg gaatcgatgc gaaaaatgta ctcctatgca aaaactacaa
     1201 tcaagcaaat gaaattattg cacaagcgaa agaagcaaaa cgtgtagtaa ttgttggtgg
     1261 tggatatatc ggtattgaat tagtagaagc ttttgtcgaa tctggaaaac aagtgacatt
     1321 gattgatgga ttagatcgca tcttgaataa atatctagat aaaccattca cagatatctt
     1381 agaaaaagaa ttgacagacc gcggtgttac tcttgcgcta ggcgaaaatg ttactgaatt
     1441 tatcacagat gaagaaggaa aagtgaaaca agtagctaca ccaacagata catttgatac
     1501 agatatggtc attttatgtg tgggcttccg tccaaataca aaactgatcg aaggaaaagt
     1561 agatacactg ccaaacgggg caatcaaagt gaatgaatac atgcagacaa gtaatccaga
     1621 catttttgca gctggagact ctgcagtagt caactacaat ccaagtggca cgcaaaacta
     1681 tattcctttg gcaacaaatg ccgttcgtca aggtttgctt gtgggaaata acttgacaga
     1741 tcataaaatg gcgtatcgtg gtactcaagg aacttcagga ctttacctat ttggctggac
     1801 gatcggttcg acaggtgtga ccaaagaaag cgcaactttg aacgatctag aagtccaagc
     1861 gactgtattt gaagataatt atcgtccaga gttcatgcca actactgaaa aagtaatgat
     1921 ggagctagtc tatgaaaaag gaacgaatcg tatcgtaggt gctcagttca tgtccaaata
     1981 cgacattaca caatcagcaa ataccatgtc tttagctgtt caaaataaaa tgactgtaga
     2041 agatctcgca ctttcagatt tcttcttcca accacatttt gaccgcccat ggaattactt
     2101 gaacctactt gcacaagcag cgctaggcga aatcgaaact gcacaataaa gaaaaagggt
     2161 tgtgacacaa ttttgtcacg accctctgtc tttttttgtg aaaagtacaa ttagttgtct
     2221 ttcttcgaat tttttgtcac aataagcagc aaagataaag ctgttacata aaagaacaag
     2281 cagctttatt tgattttgaa aagaaaggtg agaaagaaga tgaataaaga atattttgtc
     2341 caacaagatg catttttaga agattataaa agtgcttcga agaaattaac gatcaatatt
     2401 gatgagttgg cagatcaagt actctctcag ttgaatgaaa cgcaagagga atattacacg
     2461 atgccagcaa atgacgccaa agataatgtt tctcatcgtt ttcctttcag tagacgcatc
     2521 tactcagatg aaaatgacgg cacattgaat acagagtaca catatgaagg tcagccatat
     2581 gtgctagaaa ctgatgatga tctttaaaga cagtttttga aaaggccgct catccttaag
     2641 tgatgacaaa aaatcatcac aattaccgat gactgtctct ttactatctt ttaatcagaa
     2701 aaaggggtta tgacaagctt tgtcaacccc tttttt
//
LOCUS       DAFCNS010000149         2722 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFCNS010000149 DAFCNS010000000
VERSION     DAFCNS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.158"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(127..597)
                     /locus_tag="LNE23_002802"
     CDS             complement(127..597)
                     /locus_tag="LNE23_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932608.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            684..1382
                     /locus_tag="LNE23_002803"
     CDS             684..1382
                     /locus_tag="LNE23_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBK6932609.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1560..1901
                     /locus_tag="LNE23_002804"
     CDS             1560..1901
                     /locus_tag="LNE23_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932610.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1894..2565
                     /locus_tag="LNE23_002805"
     CDS             1894..2565
                     /locus_tag="LNE23_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBK6932611.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2571..>2722
                     /locus_tag="LNE23_002806"
     CDS             2571..>2722
                     /locus_tag="LNE23_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932612.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIR"
BASE COUNT          998 a          396 c          552 g          776 t
ORIGIN      
        1 gtttacatgg tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa
       61 aagccaaccg agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac
      121 tattactcag tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc
      181 aacaattttg tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag
      241 gagtttatta aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt
      301 aggaggaata tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc
      361 tttagcaatt aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga
      421 aagttctaaa ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt
      481 ataaatcata tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat
      541 tccttttatt tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca
      601 ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta
      661 taccaaaaag gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg
      721 tgactacgag cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag
      781 atattgatgc actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg
      841 aaccagattc atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct
      901 tgctggctat ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag
      961 cattcaacga aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata
     1021 tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag
     1081 atactatgcg aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag
     1141 ttatgggagt tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat
     1201 tctcaaaatt ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac
     1261 ctcgtaagag attcgatgat gctgtttcat ttattgaaat gtggttgcca gaaactgcga
     1321 tccgtatgga aattgatcaa ctgaacagac aacagagact ttttggtgat gacaatgaat
     1381 agagctgaag cgctaagaat agggacggca attgctaatt gctggtggaa atactataaa
     1441 ccaatcatcc taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag
     1501 tgactccgcc ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata
     1561 tggaaaaaga actttccact ctagatcaat atttgattga tcctgattgg ggcaagccga
     1621 aaattgagga aacaagtggt cgaaaaatca gacgaaatct tttgacgaat gaagaactag
     1681 cttgggatca agatgattta ggcaaccatg taactatttg ggatcatgtt tatcttatcc
     1741 atctatcgaa gcattcgaat aaacctgaat atatttacgt catcgaagat ggcttgattg
     1801 atgcgctaga agagtacgac agagataact tgattgatat ctcttattac ggaccaggta
     1861 agaaatacat tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac
     1921 tttgaaaaac tttttagtcg taagttaaat aaaattctca agaaaaaagg aaattttgat
     1981 tatttatctt gggctcacgc gtgggagatt atgaaaaaga atgatccaca ggcaacggta
     2041 actattaatg agtacaaaca ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt
     2101 gaggaatata aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg
     2161 gttaaaggac acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt
     2221 gttaaaccaa atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg
     2281 gctctacacg gactgggatt atatgtattt caaggggaag atattccaac accacctaga
     2341 atcgatacaa agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg
     2401 ggtaaagata tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg
     2461 ctcttagctg ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg
     2521 gagcgagcaa tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac
     2581 cattaatcga ttcatattca gcggttctga aaaagttcaa aggaaaagac ataagcgcaa
     2641 ccatcaatga ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg
     2701 atcgagtcat tgaaattcgt tt
//
LOCUS       DAFCNS010000150         2483 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFCNS010000150 DAFCNS010000000
VERSION     DAFCNS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.159"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..360)
                     /locus_tag="LNE23_002807"
     CDS             complement(<1..360)
                     /locus_tag="LNE23_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBK6932613.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLL"
     gene            545..>2483
                     /locus_tag="LNE23_002808"
     CDS             545..>2483
                     /locus_tag="LNE23_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBK6932614.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLE"
BASE COUNT          798 a          424 c          431 g          830 t
ORIGIN      
        1 gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc
       61 tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga
      121 ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa
      181 aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt
      241 aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc
      301 catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat
      361 attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa
      421 tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata
      481 tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat
      541 tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct
      601 tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt
      661 gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttgatggaca
      721 atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa
      781 aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa
      841 attcaacccc attatggcat taattaaatt attatcagat atttttgttc caattatacc
      901 tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact
      961 ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat
     1021 ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc
     1081 taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc
     1141 agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa
     1201 ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa
     1261 attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt
     1321 aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa
     1381 tggaattaca gacattattg tttggctata taacactact ggatttattg gaatgggtat
     1441 ttttggagga acatattcac ttatagtcat gactggttta catcaatcat ttccagctat
     1501 tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt
     1561 agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac
     1621 taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat
     1681 tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt
     1741 aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact
     1801 tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt
     1861 attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac
     1921 aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc
     1981 aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc
     2041 atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga
     2101 tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac
     2161 ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg
     2221 tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat
     2281 gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat
     2341 tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat
     2401 tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac
     2461 tgagggtact gatttacttg aaa
//
LOCUS       DAFCNS010000151         2316 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFCNS010000151 DAFCNS010000000
VERSION     DAFCNS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.160"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..542
                     /locus_tag="LNE23_002809"
                     /pseudo
     CDS             288..542
                     /locus_tag="LNE23_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            612..1124
                     /locus_tag="LNE23_002810"
     CDS             612..1124
                     /locus_tag="LNE23_002810"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6932615.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1121..2158
                     /locus_tag="LNE23_002811"
     CDS             1121..2158
                     /locus_tag="LNE23_002811"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6932616.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          842 a          419 c          465 g          590 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
      541 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
      601 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
      661 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
      721 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
      781 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
      841 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
      901 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
      961 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1021 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1081 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1141 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1201 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1261 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     1321 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     1381 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     1441 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     1501 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     1561 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     1621 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     1681 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     1741 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     1801 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     1861 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     1921 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     1981 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2041 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2101 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2161 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2221 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     2281 ggaaaataaa aatacgacaa ctatattgac aaacac
//
LOCUS       DAFCNS010000152         2259 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFCNS010000152 DAFCNS010000000
VERSION     DAFCNS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.161"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(148..2145)
                     /locus_tag="LNE23_002812"
     CDS             complement(148..2145)
                     /locus_tag="LNE23_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBK6932617.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          574 a          505 c          411 g          769 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaa
//
LOCUS       DAFCNS010000153         2242 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFCNS010000153 DAFCNS010000000
VERSION     DAFCNS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.162"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(558..923)
                     /locus_tag="LNE23_002813"
     CDS             complement(558..923)
                     /locus_tag="LNE23_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932618.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(999..1244)
                     /locus_tag="LNE23_002814"
     CDS             complement(999..1244)
                     /locus_tag="LNE23_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6932619.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1314..1568)
                     /locus_tag="LNE23_002815"
     CDS             complement(1314..1568)
                     /locus_tag="LNE23_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932620.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1732..2055)
                     /locus_tag="LNE23_002816"
     CDS             complement(1732..2055)
                     /locus_tag="LNE23_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932621.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          847 a          371 c          389 g          635 t
ORIGIN      
        1 ttcgtctgaa aacaaaaaaa gaaagcgtta ttattttagt ctattttcag ggggggaacc
       61 ttatgataaa aaaactattt ttactatcaa tattaacgct agcaatggca ggttgttctg
      121 actctggctt agcaactgaa ctgatcacaa aatcaactga agccacgaca gaggagttaa
      181 aatgtggaaa cgatcacagt gactacagag gcttaaatcg acgatgcatg ggaatattag
      241 gcagaagcaa ttgtaagcat cgatgtagta atcgaagaag gtgtagaaga agcaacagaa
      301 aaatcaaaag aaagatcaaa agaagcaatc tgtgctgtgc atcgtataca gacgtcgaag
      361 atgcggtact tttaaaccac gtcaggagat gttcatttat gtctagggaa gttggcgatg
      421 aaccaaaact agagtacgct tttagttttg cacgtgagga tgaagataac cctaaaactt
      481 acgacatttt agcttgatct aagatggtta aagcttttta atgaagtaag aggagataga
      541 aggcttttct agttatatta ggtaagataa attccttttt gctccaaatt tgctaaacat
      601 tctgctagat aaacttcatc gtgttccgga taaatcggca cgttgcgctc tacagtaggt
      661 aaatcaaaaa atgcttcgca tgatttgcca cgatacatag gatttttcca ctctggtgct
      721 gggctgtaac cacgtttttc catttcatcc atgacaagct gatgatattg gaataatcta
      781 taaggagaat agtgaaaaac ataatccaca gtagcatgtt tctttcccca gccatttcct
      841 cttaatgcac agcattcccg atgttgtcct aaaagctgtt gacgaggaag ctttgaaatc
      901 aatttttcat gccataatcg catagaaatg tctcctttat atatagattg ttaaccttag
      961 acgttaaatg aaaaaaccag caccaaggct ggaaaaaatc acttcagttt gcttgctgtt
     1021 tctgagcgac caattaggta atcaacggaa atatcgtaaa agtcagcgat atagattaat
     1081 tcaatgatgc ttggaatttc ttctccttct tcgtacttac gatatttgaa taaaggaata
     1141 cttaacgcgc tagaaacagc tacttgagat aaagatcggc cagttcttaa gaaattcaga
     1201 cgacaacgta aagtatccag taaaatagct tcttcgatac tcatttaatc acctttcatt
     1261 atagtagaat agttataatg actatagaaa gctacacatg caatcaatag tactcaatgt
     1321 tttttattta aacgtatcaa ataaagaatg acgatcaata aagacaaaac cagtcctgat
     1381 aattcttcta taaaaacact atcgggttca aagtagttgt aattaaaaac tttccacata
     1441 aagtagatag ccggaagagt caaaatagct aaataaaacc gttctttgtt ccaattcata
     1501 agataaacaa tacttgcgac cagtgcgacc gcaggcaccc aaaacaaaac tctcaccaac
     1561 gtaatcatgt aacggactcc tcaacgattc atataataaa agataacatg ttattgcata
     1621 catcacaaat gaaatgctag gaaaataaaa gaagaataga tcaatgaaaa atgaaaaagc
     1681 agaacagttt tctgctgata agcaggaact gttccgctga atgtgtctta tctatcgaga
     1741 aaaaatcaat ggattgatat ctaagtaatt caatacatat aaaaagagga cgatcgcatt
     1801 cactaaaaag gaaagaatga tgatcccata gcgaatggaa acatatttaa caaatacttt
     1861 tgtttgaata cgggcatatc tttttccttt atagctgagc atcatgacaa gaagcaaaag
     1921 ggtaatcaca ccttgaaaaa tcattccaat aaacagaatt cctgaggttt gataatttaa
     1981 ttggagaaaa taagctgctg ccgcaactaa atcgaataaa atcgcaataa atggcaaaat
     2041 aatcactcct aacattgaga ataaataaaa gaataacatt ttttctaaaa taagcaaata
     2101 aaaattaaag taacaaaata gttatgacac aagtaaaaga aataagaata acaatattat
     2161 tttacttata ttttgaaact tgtgagagtt tgtcattcgt gctatagttg aaaagtaaca
     2221 aaaataaatt ttatttatga tg
//
LOCUS       DAFCNS010000154         2200 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFCNS010000154 DAFCNS010000000
VERSION     DAFCNS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.163"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(165..1091)
                     /gene="cysK"
                     /locus_tag="LNE23_002817"
     CDS             complement(165..1091)
                     /gene="cysK"
                     /locus_tag="LNE23_002817"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBK6932622.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1231..2118)
                     /locus_tag="LNE23_002818"
     CDS             complement(1231..2118)
                     /locus_tag="LNE23_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK6932623.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          616 a          486 c          365 g          733 t
ORIGIN      
        1 agaaaattca attttattgt attttaatga aaaaagacgt aaaacagtcg cgttcactat
       61 tctacgtctc tattcaaata atttttctca tcataaattt ttgtgtgttc ttttcatctt
      121 taaaagtttt ttacataaac ctatctggag aaaaaccaag tgccttatat ctcttggtaa
      181 agggctgtag ataaataacg ttcaccattg tcgggaacca cagctaatac cttcttgcct
      241 tttcctaatt cttttgcgac ttttagcgcg gcagcaattg cagcgccaga tgagatgcct
      301 actaaaatcc cttcttttcg cccaacttct ctagctgttt ccatcgcttc gtctccacta
      361 atagaaagga ctttatcata cacagaagta tccagtgttt taggaacaaa acctgtaccg
      421 attccttgga ttttgtgagg tcccgcttct ttcccttcta aaatagcaga ttctgctggt
      481 tctaccccga ttaattcgat tttcggatac acacgtttta attctcggcc agcgccagtg
      541 atcgttcctc ctgtaccgat acctgcaaca aacgcgtcca agccatttac tccaaatgct
      601 tgatgtatct caggaccagt cgtcttctca tgcacaagag gatttgcttc attttcaaat
      661 tgtaatggta aaaagtagcc gttttctttt gccaaacgtt ctgcttctct gattgatcca
      721 gaaataccat ccgcaccagg agtcaaaatc aactctgcgc cgtacccttt catcaataaa
      781 cgtctttcga tactcatcgt ctcaggcatc acgatgatta ctttatatcc ttttgcaact
      841 cctaccatcg acaatccgat tcctgtattt ccagaagtag gttcaatgat agtatcacct
      901 ggtttcagta gaccatcgtg ttctgctttt tctatcatac ttaaagcaat acgatctttt
      961 acacttcctc ctggattaaa aaactcaagc tttacaaaaa catctgctga atcttctggt
     1021 acgattttat ttaatttgac gattggcgtt ttgccaatca attcggtgac tgagttatag
     1081 atagctgtca tcttcaattc ctccttgtct ttgctcttct tacttatcat aatacaatct
     1141 gttcatttat ggcgagtgat tcgtctttga ctagcaaaaa aaagacaaag atataccata
     1201 tatggtaatt tctttgcctt tttatctaaa tcaaacgttt agttcttgac caactaataa
     1261 agaataactg gacatagaca tgccattttt cgccattaga tcatcaacag acatgccaaa
     1321 tttttgtgca atatcccata gcgtatcacc tgacacgact gtatatgtct gaccattatt
     1381 tgtatttaca tctgttgttg aagatgatga cgttgaagtc gtcgtagtag tcgttgttgt
     1441 tgtacttgtg gttgttgtag ccgaacctgt tgatggcgta tcaaaacggg ttaaattata
     1501 agtattaatc aatgagttca atttggcccc ataatttgga tcggtagcat aacgacctgt
     1561 caagtacgct gttgcatcat aaaaacttgt tgtattgctt ttccacacgc cagcataatg
     1621 ataatcgccc gtggaaaaac tggttgaacg aagaacattc gcatggtctt ggaaggattc
     1681 ccaataagaa gaatattttc ggaatggctc attcatcgtt acccattgtc cgtttacata
     1741 ttctttggtt gccatataaa cgacctgtcc gttatagctt cctttcacac cgaatagatt
     1801 ataatttggc gcactagcta atccggaagt gccatatgca ctttccaaca acgcttgagc
     1861 gatcatgact gaagcataca agtcattact tgcagcaact tctgacgcag accagccgat
     1921 ttgatcgata aacgcttgag gatcagcgac tgtttgagat ccttgttcat ctgcatgaac
     1981 atgttgcggt accgctgtaa ccatcatcgg cccaactaaa agactcgttc caattaaagg
     2041 agcaatcgct tttttattga tcaccatttt tcttttaggt ctatggtgcc gggcactgcg
     2101 ggtcttcagc tcttgcattc aaattcctcc tcaaaataaa aaaacttaat aaaaagttct
     2161 cttcttaaaa caaacttaat taattaaatt atacttttaa
//
LOCUS       DAFCNS010000155         2125 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFCNS010000155 DAFCNS010000000
VERSION     DAFCNS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.164"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..159
                     /locus_tag="LNE23_002819"
     CDS             <1..159
                     /locus_tag="LNE23_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBK6932624.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="LNE23_002820"
     CDS             242..859
                     /locus_tag="LNE23_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932625.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="LNE23_002821"
     CDS             1023..1355
                     /locus_tag="LNE23_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932626.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="LNE23_002822"
     CDS             1679..1840
                     /locus_tag="LNE23_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBK6932627.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..2125
                     /locus_tag="LNE23_002823"
                     /pseudo
     CDS             1872..2125
                     /locus_tag="LNE23_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          758 a          272 c          429 g          666 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2041 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2101 gaaacctata tcaaaattaa gggac
//
LOCUS       DAFCNS010000156         2121 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFCNS010000156 DAFCNS010000000
VERSION     DAFCNS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.165"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1037
                     /locus_tag="LNE23_002824"
     CDS             <1..1037
                     /locus_tag="LNE23_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HBK6932628.1"
                     /translation="QIGAWMDKDYNITKGENVLIHKPADVTDHFRDPQMFNYEGQLYA
                     IVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGSEYMIECPNLVFVDKKPVLI
                     YCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASEIQNLDYGFEAYATQGFNTP
                     DGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDGKLYQYPVEAITSLRASSEQ
                     FTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLIVDTKEGKIILDRSKAGIQY
                     ATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVFTSRVFPENHQNGIQIKSGE
                     PTGKYFELKY"
     gene            1039..2001
                     /locus_tag="LNE23_002825"
     CDS             1039..2001
                     /locus_tag="LNE23_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6932629.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
BASE COUNT          763 a          333 c          363 g          662 t
ORIGIN      
        1 ttcaaatcgg agcatggatg gataaagact acaacattac gaaaggtgag aatgttctta
       61 ttcataaacc tgctgatgta actgatcatt ttcgtgatcc acaaatgttt aattatgaag
      121 gacaattgta tgcgattgtt ggagcacaaa atttagaaaa atcaggcttt atcaaacttt
      181 acaaagcgga tgacaataac gtggaaacat gggaggaagt tggcaacctt gattttggcg
      241 gcactggttc agaatacatg attgagtgtc caaatctagt atttgtagac aaaaaacctg
      301 ttctcattta ttgtcctcaa gggttggata aatctgagct aaattatggg aatatctatc
      361 cgaatacttt taagatctgt caatcgcttg acatagaaaa agtaaaactt gtgggcgctt
      421 cagaaattca aaatcttgat tatggatttg aagcgtatgc gactcaagga ttcaatactc
      481 ctgatggtcg tgctctgatt gtcagttgga ttggactacc tgatatcgat tatccaactg
      541 ataagtatga ttatcaggga gcaatgagtc ttgtaaaaga actttcaata aaagatggta
      601 aactttacca atatccagta gaagctatta catcattacg tgctagttct gaacaattta
      661 cagcaaaagc agaaacaaat aatacttatg agcttgaact aacttttcct ccaaatcaga
      721 aatcggagtt acttcttttc tctgatagta aaggaaatgg acttagcttg attgtagata
      781 ccaaagaagg aaaaattatt ttagatcgca gcaaagcagg cattcaatat gccactgact
      841 ttggtacgat tcgtgaatgc tcaattgata ctaaagaaac aactgctaac atttttgtag
      901 ataactcaat cattgagatt tttattaata aaggagaaaa agtgtttact agtcgtgtat
      961 tccctgaaaa tcaccaaaat ggtattcaaa tcaaatcagg cgaacctact ggaaaatact
     1021 ttgaattaaa atattaaaat ggtagcaaaa ttaactgatg tcgctgaatt agctggagtt
     1081 agcccaacaa ccgtttcacg cgtcattaat aataagggat atttatcaga aaaaacaaaa
     1141 caaaaagttc atgaagctat gaaaactctt ggttataaac caaataattt agcacgtgga
     1201 cttcaaggaa agtcacctca gcttattggt cttatttttc ctaatattag taatatcttt
     1261 tactctgaac tcattgagta tctcgaaata gagttattta aacatggata taaaacgatt
     1321 atttgcaata gtcaaaatga tcctgctaaa gagagagaat acattgaaat gcttgaagcc
     1381 aaccaagttg atggcataat ctcatcaagc cataatcttg gaattgctga ttatgagcgt
     1441 gtgagagcac ctattgttgc ttttgataga aatttagctc ctaatgtttc cattgtttct
     1501 tcagataact ttgaaggggg aaaacttgca gctaagactt tacaaaaaaa tggttgtcaa
     1561 aataccatta tgattacagg aaatgataac acggattctc ccacagaatt acgtgcacta
     1621 ggtttttctt ttcaaaatcc aaatggaaaa attttcaaaa ttcctaataa tctgtctact
     1681 atccgtcgtg aaatggaaat aaagtcaact attataaatc acaaaccaga tggtattttt
     1741 gtttctgatg atttaacagc cattctcact atgaaaattg ctcatcaact taaacttaat
     1801 atcccagaag atttaaaaat tattggatac gatggtacct cttttattga gaaatttttc
     1861 ccacaattga caacaataag acaaccaatt gatgaaattg caagtcttat tgttgatatt
     1921 cttctgaaaa aaattaaagg cgaaaagaca aataaggatt atattttacc aatctcaata
     1981 ctttctggag gaagtattta acgttaaatt aattttttaa tgtctataaa attcgttaga
     2041 aaatacaatt tgtagatatc caattgattt gtaaacaaaa aggagttgtc aacttgtagt
     2101 gacccccaaa agttagactt t
//
LOCUS       DAFCNS010000157         2056 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFCNS010000157 DAFCNS010000000
VERSION     DAFCNS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.166"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            493..714
                     /locus_tag="LNE23_002826"
     CDS             493..714
                     /locus_tag="LNE23_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932630.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="LNE23_002827"
     CDS             715..972
                     /locus_tag="LNE23_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932631.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="LNE23_002828"
     CDS             complement(1108..1710)
                     /locus_tag="LNE23_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK6932632.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="LNE23_002829"
     CDS             complement(1777..1932)
                     /locus_tag="LNE23_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6932633.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DAFCNS010000158         1995 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFCNS010000158 DAFCNS010000000
VERSION     DAFCNS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.167"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..741)
                     /locus_tag="LNE23_002830"
     CDS             complement(<1..741)
                     /locus_tag="LNE23_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBK6932634.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGICYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEA"
     gene            complement(745..1803)
                     /locus_tag="LNE23_002831"
     CDS             complement(745..1803)
                     /locus_tag="LNE23_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBK6932635.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT          641 a          425 c          328 g          601 t
ORIGIN      
        1 agcttcatta tcttgaagct ctccacgggc tgtattgatc aagatgctgc cttttttcat
       61 ttgagctaaa aatccttcat tgatcatttg atcgttttca cctgggatat gaggtacatg
      121 aatactgacg atatcgcttt tggctaataa ctcttcaagc gagtggaacg tcaatacttt
      181 tttagcagct tctgtctgat gaatatcgta gccgataact ttggctccta gtcccttaaa
      241 caaaacagct tccgtcaacc ctatcctgcc tgttcctatg atccctactg tgcaatttct
      301 gatttcacga ctaaacattt gctcatccac tcgaaaatct ttgaaagatg ttcggatcgt
      361 cgtatacgca gtatttctca aaagcatcat ggcaaaagtc aaagataact ctgctatcgc
      421 atttggcgaa tacgatggta cacgggctac tgtcatacca aaatcagctg ccgcttgtag
      481 atcgatatga ttgactccca cagtccgtgt aaaaacataa caaatcccat attcctgcat
      541 ttttgcaaga ttggtctcat ctgctataca atttcccctc aacaatacag catcatggtc
      601 ctttgctgac acgatattgt catgtgtcaa cagttcttca taaagactga gatcataatg
      661 gtaaatattc aatttttcaa aaaaatctat ctcgttcgga cgaacaccgt aacatgcgat
      721 tctcatactt tcttcctcca tttcttatga aaataaacct atgccatgaa tcatttgtaa
      781 tacaacgatc catacaggtg tcagtaccac agcaaaaata gttgacagta aggatgcatt
      841 ggaggctaaa agtgcctccc tatcaaaact gatagcatag gctgctgcca ctgttgcagc
      901 tggtgtagcc atcatgataa cgattgttgc cagtgcttca aaattgactg gtagaatatt
      961 cgttgccgtc aaaactgcta gaatcacgat attcaatgca ggtacaaata ccactttgac
     1021 tagactgtaa taccatgaag ttttttcaga tgcagctttt ttgaagctta cttcacctaa
     1081 tgttgcgccg attgctagcc acgccaatgg tgaacttaaa ccagctaaat aatccatcgg
     1141 ctgaaatagc cacggtgcag tctggtcaat tcgcaaaaac gctacttggc ttatctctcc
     1201 ttctgcacct gtaactgaca cttgtggcag atacccttgg aacagccaaa tgaaaaggcc
     1261 ggcaaaagta gcgataatga ttggattgac gaacatcgct ttgatatttt tagcttccat
     1321 cttcaagcca ctcattttta tgtatccata agaatacaaa aagattcggt agccaatatt
     1381 gaaaattgag gaatacatca cgccgacagg accataaatc gcactgacga taggcgttcc
     1441 gaaaaaggtc gtagaaccaa agatcgtcag gacacgcaac gttgtttgct tatccccttt
     1501 atagcttaag aaaaaaggtt ttgaaactag aataagaata atataaataa ctaatcccca
     1561 aatcaagaca ttgattcctt gcatcagtaa ttctgtattg atatcttgca tgaaagctcg
     1621 aaatgccagt gctggcaaag ccactgttaa gacgactttc gatagaattt ttccaacaga
     1681 ttcagaaaaa atcccctttt ttctacaaac aaaccctaat aatataataa aaactgttga
     1741 agtgattgca ctgacaatcg acatatctgt caaagtcgat cgaaaaatgt caaataagtt
     1801 catctttctc ctcctaatta tgttcattta ttcacaataa aaaaacgctt ttattttgct
     1861 aatacgtatt aacattttta attttatctt tacattgtta cttattgaac aataggaaat
     1921 taaagaaact tttgaaactt atgtaaagtg atttttacta gaacacaaaa aaaaccttaa
     1981 aaccaaactt aagaa
//
LOCUS       DAFCNS010000159         1989 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFCNS010000159 DAFCNS010000000
VERSION     DAFCNS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.168"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..1851
                     /locus_tag="LNE23_002832"
     CDS             127..1851
                     /locus_tag="LNE23_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK6932636.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          780 a          241 c          347 g          621 t
ORIGIN      
        1 agtttgtaac aaacatatat tttttatatt acaatatttc aacaaattaa aaaaaattat
       61 atcttcttaa acaatgataa tattaaaatg caagcgcttg taaaaaataa ggagaagagg
      121 taaaaaatga agaaaaaact attaacttta tttcttgtga tgtctttctc aatcgttttg
      181 agtgtgtact acaatactat tgttttttca gaacaaataa actacacaga agaacagtat
      241 aatatagaaa aagaaaaagc tttaaaagtt ggttatagtg aagaacagtt taagcagatt
      301 atggaaatac ctacaaattt atcaaatgaa aatagtgaaa caagaatagt aaattatact
      361 atgacttcta atcaaaccaa agtcattaat aaagcaatgg agcaaattgg aaagccatac
      421 gaatggggag catcgggacc aaattctttt gactgtggtg gtttagtgaa atatgtttac
      481 aaacaagctg taaatattga actgcctatg ggaaccacca atcaagagca atacggtaca
      541 gaggtaagtt taaattcact taagcctggt gatttattat tttacggcaa tcgaggtgca
      601 acttatcatg taggtatata taaaggtaac ggtgtaatga ttcatgcacc gcaacctgga
      661 gaaactgtaa aagaggttaa catacaatat ttttatccta actttgcaaa aagaattttg
      721 cctgatgaac ctgactaccc ttatattgac tataataaaa tggtaactgt tacaaaagct
      781 tggtcaatat ggaatgattt acaatttagt catgaaatta agaaagctat tattggagat
      841 aattacaaga tagggaaggt ttatactaat ccagaaaata ataataaata tggagaaata
      901 ttagtaagta ataaagtata tggatacatt aatttttatg ctgttaaaga gttgacctct
      961 gttcaaataa acagatattt aacttctaaa gatagtggtc aaccaacttg gggaaatcta
     1021 gagtgtacta taagtaaagg acaaactaca aaagataaaa tatattttgt taaaggagca
     1081 tataatttag gggatggaaa gtatctatat tctatttata aagatcagga ttcctctgaa
     1141 tggttagggt atttaaaagc acatgttagt ttagcctata ctcctattga agaaattaat
     1201 aaaaacgtaa ctgttactaa aaattggtcg atttggaata atctacaatg ggaaaaggaa
     1261 attgaaaagc ctcagattgg ttctgtattt tcagctagat taaagttaac caatgtatcg
     1321 aataatgctg tctattacaa gctttataaa tctggaaaat tttatggtta tatcaatgct
     1381 gaggctgtca aagaccttac aacaacaaag cttaataagt atgttacatt tagtgttaat
     1441 aatgaagatt tttggtcttc tttagatgta aattattcaa aaggaaaaac tgaaagaggc
     1501 cgagtatttt acattagtat ttcatataat accgcagata accaaccgat ttattcagta
     1561 tatacagatg aaacatgtac ggaatggcga gggtattata agggaaacaa ctttgaagac
     1621 actcaaatta ctatgcttga gaataaaagc gtgaaagtaa caaaaagtgg gtatacagta
     1681 tggggagact tgaatttttg gactaagtca ggaatatcga acacaggaga tatatacacc
     1741 actgaccgaa aattttataa ttttaccaac aatgcttact attatgaatt gaaaaaagat
     1801 gggagggtct atggctatat aaatagtgaa gcagctgtag agatgaacta aaatattgct
     1861 gtcccttaat cttatggggc agcaatattt ttatattatt aatcattttt ctgaagcgag
     1921 ggtagaagaa ttaatcaata gtttttcttg ttttgtaata taatagttta attaccaaaa
     1981 attatttaa
//
LOCUS       DAFCNS010000160         1979 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFCNS010000160 DAFCNS010000000
VERSION     DAFCNS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.169"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(325..528)
                     /locus_tag="LNE23_002833"
     CDS             complement(325..528)
                     /locus_tag="LNE23_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932637.1"
                     /translation="MSQLGKRYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="LNE23_002834"
     CDS             complement(728..1744)
                     /locus_tag="LNE23_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK6932638.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAFCNS010000161         1907 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFCNS010000161 DAFCNS010000000
VERSION     DAFCNS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.170"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(287..360)
                     /locus_tag="LNE23_002835"
     tRNA            complement(287..360)
                     /locus_tag="LNE23_002835"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(324..326),aa:Ile,seq:cat)
     gene            complement(549..1568)
                     /locus_tag="LNE23_002836"
     CDS             complement(549..1568)
                     /locus_tag="LNE23_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBK6932639.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1579..1785)
                     /locus_tag="LNE23_002837"
     CDS             complement(1579..1785)
                     /locus_tag="LNE23_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK6932640.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          561 a          390 c          324 g          632 t
ORIGIN      
        1 ataaaagagt ttaaagcgta acacacttat ggggaagtgg cttggggtgc gctttaaact
       61 cttctttatt atcatctcac aatttaaccc aaatgtcttt ctatttaaaa atcaaagtaa
      121 aacttttcaa atatacagaa atataactat gtgaaacatc ctttcattaa tccataaaag
      181 gatacataaa aaagccactc atttgagtgg caatgaagaa aagctttagc ttgtataata
      241 ctcttcaaaa aattctaaca cagaacgatt caaatggcta cgttaatgta ccctgtagga
      301 ctcgaaccta cgaccggacg gttatgagcc gtctgctctg accaactgag ctaagggtac
      361 tggttgttgc cacataaagc cataaacaat caaccagtag aatgtgtggc aacaaacctg
      421 ttatcgcata gcttggagtg tgactattat gggtgatagt gaagatatgc gataacatca
      481 ctattttatc gaatgatttt tatagttgtc aatatagtta tgtaatgctc ctcaacgagg
      541 agctattttt atcgtttagg aatatttaaa taccaacgtt tgtcatggaa atcttgcgca
      601 ccgcctttag tgtttccctc tggatcattc gttgcccgca tcatgacata gactttctta
      661 ttagggaagt tacgcatgtt aaaagataca tgatagccaa catttccaga agtattataa
      721 gcttgattta catctgatct ataaattcca tcagctctta ctcgagctaa ttctttccca
      781 gtattgtaat ccataatgaa gatatactcg tatttatagt tagcaatgtg ccatccagct
      841 acatgcaaat ttgcattttc gatttctcca aactgatcaa tgtgggcgta attcgttcca
      901 tctgtcagtg taggattagc tgcaccagct cgtgttggat caatgacagg cttgttttca
      961 gaagttgttg gattttcatc ggtaaatcca tgagctaaat catatgctaa tttttcttta
     1021 cttacgccca tttcagaaag ataaccgtaa ggatctgtat gatcgcccca gatgttttgt
     1081 gttacccata aatgcgattt gattcccggt tggttataag gagtgtccaa cgttaatgga
     1141 ataccatatt tcattgctga atctctagcc aattcaacgt atgccttgta gtttttctca
     1201 aacgttgctt tatcatgtgt gtgttgtaac tcaatctgca caggactgtt ggcattagca
     1261 tacgaaccag caccgtactg tacataacca ggttgaccga cttgataaac aattccgccg
     1321 tctcccacaa tataagcagt ataagcgcta gtccatgaac gttgcatata ctgcgcttca
     1381 ttgcgtcctg ttgctgtttc attagccgtt tcatgcagta aaatatactg attatttgct
     1441 acttgtgagc taccttcatt tgcgcccaaa ttaaattcat tgttgatagt ataggcaaat
     1501 ccgttaattg gcaataaaaa aagagccgtt aacaggctca tcgcagtaat agtaattttc
     1561 tttttcatat ctatcctcct atttatctga tgaaattttc ttatacgttc gatttgatac
     1621 acctaaaata ctccctagaa atgctccaaa acctgtaata atcatcaccg ttatatctgt
     1681 gtaccgccag ttaacacctt ttccgacaag accgacaaaa gtcgctaaag cggggataac
     1741 tactatagcg atccatttca aaatatcaaa ggttctattg ttcatactat ccacctccct
     1801 tcattggttg ggctactttc tttttcaatc gcttgttttc ttctatcaat atttctcttt
     1861 ctgaaagcaa ggcatcaatt tgcccgtcca gttgttcaat tttatct
//
LOCUS       DAFCNS010000162         1847 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFCNS010000162 DAFCNS010000000
VERSION     DAFCNS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.173"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            700..>1847
                     /gene="ltrA"
                     /locus_tag="LNE23_002838"
     CDS             700..>1847
                     /gene="ltrA"
                     /locus_tag="LNE23_002838"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK6932641.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
BASE COUNT          730 a          281 c          384 g          452 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaaga
//
LOCUS       DAFCNS010000163         1842 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFCNS010000163 DAFCNS010000000
VERSION     DAFCNS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.174"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            467..1639
                     /locus_tag="LNE23_002839"
     CDS             467..1639
                     /locus_tag="LNE23_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBK6932642.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          642 a          341 c          301 g          558 t
ORIGIN      
        1 aaaagttcaa aagatcaagt gtgcgcttcg ctcccactcc cctcgacgtt ttagtagcct
       61 ttccctcact tcgttcagtc caagccaact aaaaagtttt cgggatacat tctccttctc
      121 ttcctaataa tcaattaaaa ttttaatctt tatatttctg ctaatcactc aggaaacagt
      181 acagaaaaac aaacacgtat catagatata aatgtaatgg catagtgcgg gttttatttt
      241 cagcctgtat catagctaaa caaatcgagt tgtgtgtccg tttttgggcg ttctgctagc
      301 ttgtttaaag tctcttgaat gaatgtatgt tctaagtcaa aaaactctga cagcgccttt
      361 atatagataa agtccgtata attgtgtaaa agtaaaaagg ccatataaca gtccttttac
      421 ggtacaatgt ttttaacgac aaaaacatac ccaggaggac ttttacatga cccaagtaca
      481 ttttacactg aaaagcgaag agattcaaag cattattgaa tattctgtaa aggatgacgt
      541 ttctaaaaat attttaacaa cggtatttaa tcaactaatg gaaaatcaac gaacagaata
      601 tattcaagca aaagaatatg aacgaacaga aaaccgacaa agtcaacgaa atggctatta
      661 tgagcgcagc tttacgacac gtgtaggcac gctagaatta aaagtaccca gaacacgtga
      721 tggccatttt tcacccacag tgtttgaacg ttatcaacga aacgaaaaag ccctcatggc
      781 ttcaatgttg gaaatgtatg tatcaggcgt ttcaactcgt aaagtatcaa aaattgtgga
      841 agaactttgt ggtaaatccg tctctaagtc cttcgtttct agcttaacag aacagctaga
      901 acctatggtt aacgagtggc agaatcgttt attatcagaa aaaaattatc cttacttaat
      961 gaccgatgta ctctatataa aagtacgaga agaaaatcga gtactctcaa aaagctgtca
     1021 tatagcgatt ggaataacca aagatggcga ccgtgaaatt atcggcttca tgattcaaag
     1081 tggcgaaagc gaagagacct ggacaacatt ttttgaatac ctaaaagaac gcggtttaca
     1141 aggtacggaa ctcgttattt ctgatgcgca caaaggatta gtctctgcca ttagaaaatc
     1201 cttcaccaac gtaagttggc aaagatgcca agttcacttc ctaagaaata tctttaccac
     1261 cattcctaaa aaaaattcaa aatctttcag agaagctgtt aaaggaattt ttaagttcac
     1321 agatattaac ttagcgcgtg aggctaaaaa tcgattgatt catgattata tcgatcaacc
     1381 aaaatattca aaagcttgcg catcattgga tgatggattc gaagacgcct ttcaatatac
     1441 cgtacaagga aattcccaca atcgactaaa gagtaccaat ctaattgaac gactgaatca
     1501 agaagtacgc agaagagaaa agattattcg catcttcccc aatcaaacat cagccaatcg
     1561 cttaattgga gccgttctta tggacctaca tgatgaatgg atttattctt caagaaaata
     1621 catcaatttt gataagtaga aatggtaaaa acattgtata gcattttaca caggagtctg
     1681 gacttgactc acttccttta ttatttttca tttttttaaa ctatttaata ctaatgtctt
     1741 ttataatagc ttttcatata ttatataatc aatctttata agtcctttta taaatttctt
     1801 ttctaccatt ttcgataaat tcctgtttaa tatttttaat tc
//
LOCUS       DAFCNS010000164         1752 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFCNS010000164 DAFCNS010000000
VERSION     DAFCNS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.176"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            383..1633
                     /locus_tag="LNE23_002840"
     CDS             383..1633
                     /locus_tag="LNE23_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK6932643.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          578 a          335 c          309 g          530 t
ORIGIN      
        1 ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga ataacacatt
       61 cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat gtatggaaag
      121 gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt tagtctgtta
      181 cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac tagactttca
      241 aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt aggcgaggtt
      301 gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc caaaatttat
      361 atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta gctctgaaaa
      421 actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg aaatctctgt
      481 tgccaatgac atcgaaggtg cttcattcat acgagaaaca attcttgaat tcaataacag
      541 ctaccacttt gatcaaattg tgattggcat ggagtcaacg tccatgtaca gcttccatcc
      601 ttccatgttt tttcatgaag atgagaaact aaagaagctc aacacacttg taacgattga
      661 aaatcctttt cgggtgaaac aatttagtcg catgtttgat gagaataaaa ctgatcgaaa
      721 cgatgcatta agaatcgctg atttcttgcg tattcaacga ttcacaactt cccctatcaa
      781 agaagagaaa tacatggcct tacagcgttt aacacgtact cgctatcaac ttattaagca
      841 attgattcgt acaaagcaac attttcttga aaatctaacg tacaaatgta atacgcttgc
      901 tcgagaaatg cgagacgaat cgacgagtct ttttagtgca accctcattt cactcatgac
      961 ggaagatttc acattggatg aacttgctga gcttccttta gaagctttct gtgatttact
     1021 tcaagaaaaa ggaaaaggcc gtttcaagca accagaaaaa atcgctaaag caattcaacg
     1081 tgcaattacc atgagttacc gcctaggtgc tcttgcacaa gaatcaatta atgttgtttt
     1141 aagtgttctc gtacgagaaa tacgagcact tgaaaaaaat atcaaagaat tagataaagc
     1201 catcgaacaa gtaattgttg tcattccaga atatcaatgc ttaaccagta ttccaggcgt
     1261 tggtaaagtc tatgctgccg gtttaatcgc tgaaattggt caaatcgaaa gatttgaaga
     1321 tcagacaaaa ttggcaaaat acgctggatt aagttggaaa gtgaaccaat ctggaaacta
     1381 tcagtcgcaa aatacacctc tgacaaaaca aggaaatcga tattttagat actacttagt
     1441 tgaagctgcc aactctgtaa aaaattatct tcctgaatac aaagcttttt atcaaagtaa
     1501 atataaggag gttccaaagc atcaacacaa acgtgcactc gtcctaaccg caagaaaatt
     1561 tacgcgtctg gtggatacgc tactacgtaa acaccaactc tatacgccac caaggagtgt
     1621 gatagagaaa taacaatccg ttattgacgc aaatccttga atcaacccga aaaaaatttg
     1681 attttgatcg ggtctagttt cgtgcgcttc aaaacatatt actcaataaa gaaaatctta
     1741 aatttcatct tg
//
LOCUS       DAFCNS010000165         1739 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFCNS010000165 DAFCNS010000000
VERSION     DAFCNS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.177"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..1235
                     /gene="aph(2'')-I"
                     /locus_tag="LNE23_002841"
                     /pseudo
     CDS             1..1235
                     /gene="aph(2'')-I"
                     /locus_tag="LNE23_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1219..1611
                     /locus_tag="LNE23_002842"
     CDS             1219..1611
                     /locus_tag="LNE23_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6932644.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          716 a          133 c          297 g          593 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      601 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      661 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      721 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      781 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
      841 ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa
      901 ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt
      961 ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta ttgttagatg
     1021 gcaataatag attaactgga ataattgatt ttggagattc tggaattata gatgaatatt
     1081 gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat tttggagaag
     1141 atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat caagatatag
     1201 ttgaagaata ttatcctatt gaaactattg tttattgatg ataatatggt ttttgatatt
     1261 gataatttaa aaggatttct taatgatacc agttcatttg ggtttatagc taaagaaaat
     1321 aataaaatta taggatttgc atattgctat acacttttaa gacctgatgg aaaaacaatg
     1381 ttttatttac actcaatagg aatgttacct aactatcaag acaaaggtta tggttcaaaa
     1441 ttattatctt ttattaagga atattctaaa gagattggtt gttctgaaat gtttttaata
     1501 actgataaag gtaatcctag agcttgccat gtatatgaaa aattaggtgg taaaaatgat
     1561 tataaagatg aaatagtata tgtatatgat tatgaaaaag gtgataaata aatgaatata
     1621 gttgaaaatg aaatatgtat aagaacttta atagatgatg attttccttt gatgttaaaa
     1681 tggttaactg atgaaagagt attagaattt tatgatggta gagataaaaa atatacatt
//
LOCUS       DAFCNS010000166         1653 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFCNS010000166 DAFCNS010000000
VERSION     DAFCNS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.178"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1143
                     /locus_tag="LNE23_002843"
     CDS             <1..1143
                     /locus_tag="LNE23_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6932645.1"
                     /translation="NHEVKTIDADKKTVTVTSASDNETKEYPYDKLILSSGVKPNNLP
                     VPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVGAGYIGIEAAEASRKAGKEVVL
                     LDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKIEAFTGNGKVEAIKTSEKEIPA
                     DLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTNLPDVYAVGDATLAYSIPARKK
                     MPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSALSVFDYHFAASGLNSFTAAKA
                     GVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMTHQLLGGAVLSTYDVTGQGNVL
                     ALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQALGEEPFVE"
     gene            1424..1653
                     /locus_tag="LNE23_002844"
                     /pseudo
     CDS             1424..1653
                     /locus_tag="LNE23_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          591 a          293 c          356 g          413 t
ORIGIN      
        1 aatcacgaag taaaaacgat cgatgccgat aaaaaaacag taacggttac tagcgcatca
       61 gataacgaaa caaaagaata tccttatgac aaattgattt tgagttctgg tgtcaaacca
      121 aacaatctgc cagtaccagg agcagatcta gaaaatgtct acttgatgcg tggatacaat
      181 tgggcaacca aaatcaaaga aaaattaaat gatccggcta ttaaaaaaat cgctgtcgta
      241 ggtgcgggtt acattgggat cgaagcagca gaagcaagcc gaaaagctgg gaaagaagtc
      301 gtattgcttg atgtgatcga tagaccttta ggcacttatt tagatgctga gatgacagat
      361 attttagaac aacacctaaa tgaaaacggt attgaagttt tgacaaatgc aaaaatcgaa
      421 gcttttactg gtaacggaaa agttgaggca atcaaaacaa gtgaaaaaga aatcccagca
      481 gatctagtca ttcaagctgc cggtgtgaag gcaaatacag aatggttaaa agggatcgtc
      541 gacttagatg aacgtggctg gatccaaaca aatgaatact tgcaaacgaa tcttccagat
      601 gtgtatgcag taggagatgc tacacttgct tattctatcc cagcacgcaa aaaaatgccg
      661 atcgctttag cgactgttgc acgtagagaa gcacgttatg tagtaaaaca tctttttgaa
      721 gaagtaccaa acaaaccgtt cggcggagtc gtaggttcct ctgcattaag tgtctttgac
      781 tatcattttg cagcaagcgg attgaatagc tttacagcag caaaagcagg agtagatgta
      841 cgagtgtctt attatgaaga tacgttacga ccaaaatatg taccagaaga acaaggaaat
      901 ccaaaagtgt ccatccaatt attctcggat ccaatgaccc atcaattgct aggaggagca
      961 gtattatcta cctatgatgt tacaggtcaa ggaaatgtat tggcactagc gatccaaaac
     1021 aaactgactt tagaagaact agcagaagct gacttcttct tccagccagg ctttgaccga
     1081 caatggagca tgttgaatct agccgctcaa caagcgttag gagaagaacc atttgtagaa
     1141 taagaaaacg aaatatttga aaagaggttg tgaaaaagct gccttgcatc aagtaataag
     1201 aacagccaag taaaaacagc ttcttatgtt tttaacttgg ctggacttat taccgcagat
     1261 gcaagctttt gaacaccgtt tattttcaaa aaagggactg tgacacgatt tgtgtaccga
     1321 cccccaaaag ttagaccaaa aaatctaact tttggaggtc ggtatttttt atggttaaat
     1381 atacttttga acttaaatta aagatcgttc atgattattt ggatggcaaa ggcgattgga
     1441 ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaaattcggg agaagcacaa
     1501 ggattatggc tgtttacgga tgaccaatga actaagaaac cgcggtttct ctatcaataa
     1561 aaagaaagtt cagcgcttga tcaagaagct aggaataaaa gtaacggcat acacgcgtaa
     1621 atcacgtcgc tacaattctt atcgaggaaa agt
//
LOCUS       DAFCNS010000167         1644 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFCNS010000167 DAFCNS010000000
VERSION     DAFCNS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.179"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(61..279)
                     /locus_tag="LNE23_002845"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="LNE23_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="LNE23_002846"
     CDS             complement(545..739)
                     /locus_tag="LNE23_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932646.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="LNE23_002847"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="LNE23_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          582 a          243 c          218 g          601 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atat
//
LOCUS       DAFCNS010000168         1623 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFCNS010000168 DAFCNS010000000
VERSION     DAFCNS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.180"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1623
                     /locus_tag="LNE23_002848"
     CDS             <1..>1623
                     /locus_tag="LNE23_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6932647.1"
                     /translation="VDENRAKQRLRIYYAKTSVIGNTIEVKAEPIFNDIRKSVLNKYD
                     SGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKANFLEFFGGKEGSIL
                     DRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQSVLVGVYPFISSSS
                     EGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAAKDWLKTNIDKQKP
                     QVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSARIVEYTFNVLTNS
                     YDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDIVDHQTDMITGQNG
                     GYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGINGTYETAWTLDGGFN
                     ASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKDIMELEARDYVSAD
                     AGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFRYSNASYVTSGDGS
                     SLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPG"
BASE COUNT          554 a          232 c          354 g          483 t
ORIGIN      
        1 gtagatgaaa atcgagcaaa acagcgtcta cggatttatt atgcaaaaac ttctgtaata
       61 ggcaatacga tagaagtaaa agctgaacct attttcaatg atataagaaa atcggtgttg
      121 aataaatatg acagcggaac agaaaagatc actgctactc aggcatggca aaacgcaaaa
      181 gttttagcga aaccagctat cccttcgcag ttttctttct cgtcattagt agatacgctt
      241 gctaatgtga agatagaaaa ggcgaatttt ttagaattct ttggtggaaa agagggatct
      301 attctagatc gatttcatgg tgagtttcta aaagataata acacattacg tcatgaaaaa
      361 agtctaggta cggatcataa aatcaaagcg atttatacta aaaacttaac tggtcttgac
      421 ttagagattg atgctcaaag tgttttagtt ggagtttatc cattcattag cagctcttca
      481 gaaggagaag acgagatcac tctaccagaa gaagttattt tcacggatta cgtggatgat
      541 tatcctgctg gatatgtttc ttttgttgat tttaaagaca aagcgactga tgtagccaca
      601 ttaagggaag ctgctaaaga ctggttgaaa acaaacatag ataaacaaaa accacaagtg
      661 agtggttcga ttgaattagt accattgagg catcaaagag gctatgaaaa atttgttgat
      721 ctagaaaaag tttcgatggg tgacggagta gatgtgtatc atccacagtt aaaagtgaat
      781 atgtcagcga gaattgtgga atatacgttt aatgttctaa ccaattcata cgataaatta
      841 gttgtaggaa acgtcaaaac aaacttctta gaaaacacag agaataatgt cagcaatttg
      901 attaatgatg ccattgatca attgaaaaac ggtggcgaaa tcagtgattt actcaatgat
      961 attgtagatc atcaaactga tatgattact ggtcaaaatg gtggttatgt tttattagat
     1021 cctaaagaag cgcctagtcg tattttgatt atggacacac cagataagaa taccgcaagg
     1081 aatgttttac aaatcaacaa tgctggtatt ggtttttcta aaactggcat taatggaaca
     1141 tatgaaacgg catggacgtt agatggcgga ttcaatgcct cgtttattac ggctggtgaa
     1201 atagtaggaa ttactattag aggtactaca ttaattagtg atggtgctga ttatagaaca
     1261 agtattgcta atggcaaaat gacttggtac tcaaaaaaag ttaacaaaga tattatggag
     1321 ctagaagcac gtgattatgt aagtgctgat gccggtattg tatcatacac catgaaaact
     1381 ggtggtggtt tcatgattag aaatccacag ggtaacttgg tttttagtac gtgggataat
     1441 ggtaataaca gaccgtttct atcttttggt gcgcccaatt tcaggtatag caatgctagt
     1501 tatgtaactt ctggcgacgg tagttcttta agcattaatg gtagtgcggg taactcatgg
     1561 gaatttaagg tagctggtag gactatgaaa tttactagtg atggtatgct aacgttacca
     1621 ggt
//
LOCUS       DAFCNS010000169         1573 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFCNS010000169 DAFCNS010000000
VERSION     DAFCNS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.181"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DAFCNS010000170         1562 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFCNS010000170 DAFCNS010000000
VERSION     DAFCNS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.182"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            46..105
                     /gene="ermCL"
                     /locus_tag="LNE23_002849"
     CDS             46..105
                     /gene="ermCL"
                     /locus_tag="LNE23_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HBK6932648.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            153..887
                     /locus_tag="LNE23_002850"
     CDS             153..887
                     /locus_tag="LNE23_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HBK6932649.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          584 a          247 c          211 g          520 t
ORIGIN      
        1 cttgtgctat aattattcta atttcataaa aaggagaatt aatatatggg catttttagt
       61 atttttgtaa tcaacacagt tcattatcaa ccaaacaaaa aataagtggt tataatggat
      121 tgttaatata aacctcatag gagtgggtta taatgaacaa aaaaaatata aaagatagtc
      181 aaaactttat tacttcaaag catcatataa atgaaatttt gagaaatgta catttaaata
      241 caaatgataa tattattgag attggttcag ggaaaggtca tttcacgttt gaattagcta
      301 aaaggtgtaa ttatgtaacc gccattgaaa tagatcctaa attatgtagg ataactaaaa
      361 acaaacttat tgaatatgag aacttccagg ttatcaataa agatatttta caatttaagt
      421 ttcctaaaaa taagtcatat aagatatatg gaaatatacc ctacaatata agtacagata
      481 taattcgaaa aattgttttt gaaagcacag ctacagaaag ttatttaata gtggaatatg
      541 gatttgctaa aaggttgcta aatacaaatc gttcactagc actattttta atgacagaag
      601 ttgatatatc catattaagt aaaatcccta gagaatactt tcatccaaaa cctagagtta
      661 atagctcgtt aattgtatta aaaagacacc cttcaaaaat atcactcaaa gatagaaaac
      721 aatatgaaaa ttttgttatg aaatgggtta acaaagaata cataaaacta ttttccaaaa
      781 accaatttta tcaagcctta aaatatgcaa gaattgacga tttaaacaat attagctttg
      841 aacaattctt gtctcttttc aatagctata aattatttaa tagataagtt aaggggtgca
      901 taaactgcat cccttaactt atcttatttt gcttaaacaa tcttggcccg taaactgcat
      961 aactcatatg taaatcgctc ctttttaggt ggcacaaatg tgaggcattt tcactatttc
     1021 cggcaattac ttccatgtaa agtataaccc actatacttt gcaagcaaag tgtgggctcg
     1081 ccgaggggtc ttgcagacgg ctctttcggc tcttttgagc cgagtatcag cgcaccctta
     1141 tggtgcaatt agctgatacc tgtttgacga tttgctgccc taccttttcg gtaggtatgc
     1201 aaacctcaaa ccaaaataaa aacccccttc gacttcgtca gggggttttt atttatataa
     1261 atctccgtaa ataatcgcga tttaataaca tcttcaattt tatttttttg cgtattttag
     1321 aaatgttttc actaaaaaat atattataca aaaaatacag taatcgatta taattctttg
     1381 ccagttaaat atcatatcgt tattaaaata tcggaaaaaa atatcaacaa caataaatgc
     1441 aaaagaagac cataaaaatt gtttattact catacatact ctccatttct atcattttta
     1501 aatttaccag tcatctctca aaaatctcct agctattttt cttttttaac cacataccgt
     1561 ca
//
LOCUS       DAFCNS010000171         1561 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFCNS010000171 DAFCNS010000000
VERSION     DAFCNS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.183"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            132..344
                     /locus_tag="LNE23_002851"
     CDS             132..344
                     /locus_tag="LNE23_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932650.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="LNE23_002852"
     CDS             506..1024
                     /locus_tag="LNE23_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBK6932651.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="LNE23_002853"
     CDS             1031..1543
                     /locus_tag="LNE23_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932652.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          615 a          181 c          242 g          523 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 t
//
LOCUS       DAFCNS010000172         1531 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFCNS010000172 DAFCNS010000000
VERSION     DAFCNS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.184"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1342
                     /locus_tag="LNE23_002854"
     rRNA            <1..1342
                     /locus_tag="LNE23_002854"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1437..>1531
                     /gene="rrf"
                     /locus_tag="LNE23_002855"
     rRNA            1437..>1531
                     /gene="rrf"
                     /locus_tag="LNE23_002855"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          428 a          329 c          449 g          325 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg t
//
LOCUS       DAFCNS010000173         1519 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFCNS010000173 DAFCNS010000000
VERSION     DAFCNS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.185"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1469
                     /locus_tag="LNE23_002856"
     CDS             <1..1469
                     /locus_tag="LNE23_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBK6932653.1"
                     /translation="LTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNLFSDEEIS
                     VSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGR
                     TKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVA
                     ETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQ
                     EEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFT
                     GYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVL
                     EKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEP
                     IFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPV
                     KYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          509 a          273 c          317 g          420 t
ORIGIN      
        1 atcttacaac tgaaatccaa ctattgaatg aaaaggtcaa ttatttaacc aacaaattat
       61 ttggacgctc taaagagact ctctttgagg aaacgaatgg gcaattgaat ctttttagtg
      121 atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca gtaaaaggac
      181 atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata acagagaaag
      241 aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag atgaaagata
      301 tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg gattgtctca
      361 cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt tcttctttta
      421 aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat agtttagtgg
      481 cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa gagaactatt
      541 ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg catattaaag
      601 cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt aatcaagaag
      661 agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa acagatacct
      721 attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat tatcaccacg
      781 acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca gggtatttac
      841 actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc tattgtttcg
      901 cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac acggatattc
      961 ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag aaatggaaag
     1021 acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca ttatttattg
     1081 ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg gatgcagccg
     1141 ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat ggtcgtttag
     1201 aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga cggaaaaatt
     1261 ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg agtgtatata
     1321 aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta tttgataaga
     1381 taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct tggaacaagg
     1441 atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt ctattctata
     1501 ggtcgggtta tttgacgta
//
LOCUS       DAFCNS010000174         1516 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFCNS010000174 DAFCNS010000000
VERSION     DAFCNS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.186"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..695
                     /locus_tag="LNE23_002857"
     CDS             288..695
                     /locus_tag="LNE23_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932654.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="LNE23_002858"
     CDS             709..1110
                     /locus_tag="LNE23_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932655.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="LNE23_002859"
     CDS             1112..1483
                     /locus_tag="LNE23_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932656.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          575 a          185 c          264 g          492 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat tttttt
//
LOCUS       DAFCNS010000175         1461 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFCNS010000175 DAFCNS010000000
VERSION     DAFCNS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.187"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            102..1397
                     /locus_tag="LNE23_002860"
     CDS             102..1397
                     /locus_tag="LNE23_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK6932657.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          460 a          289 c          271 g          441 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      721 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      781 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      841 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      901 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      961 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
     1021 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
     1081 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag
     1141 atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata
     1201 caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca
     1261 gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg
     1321 cgtacggctt tcgcaacttt tacaatttca aactacgaat tatgattcgt catggcaaaa
     1381 catttttaac caaataaaca gaacagaaag aggttttctt ctttctgttc tgtccaaatt
     1441 attctaagat ttctctttac c
//
LOCUS       DAFCNS010000176         1456 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFCNS010000176 DAFCNS010000000
VERSION     DAFCNS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.188"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            109..1041
                     /locus_tag="LNE23_002861"
     CDS             109..1041
                     /locus_tag="LNE23_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBK6932658.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1456
                     /locus_tag="LNE23_002862"
     CDS             1113..>1456
                     /locus_tag="LNE23_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932659.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          640 a          169 c          228 g          419 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaaga
//
LOCUS       DAFCNS010000177         1455 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFCNS010000177 DAFCNS010000000
VERSION     DAFCNS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.189"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..358
                     /gene="tnpA"
                     /locus_tag="LNE23_002863"
     CDS             <1..358
                     /gene="tnpA"
                     /locus_tag="LNE23_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBK6932660.1"
                     /translation="LRKDLGKILRKLCEMKDVEIIEAHAMLDHIHMLVRVPPKICISS
                     FMGYLKGRSAVLIHEQHANLKYKYGNKSFWSKRYYVSTVGLNQKTNEKYIREQEADDR
                     IRYIISKREYVEPFKK"
     gene            623..721
                     /locus_tag="LNE23_002864"
                     /pseudo
     CDS             623..721
                     /locus_tag="LNE23_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          532 a          229 c          265 g          429 t
ORIGIN      
        1 attacgaaaa gatcttggaa aaatacttag gaagttgtgc gaaatgaaag atgtggaaat
       61 catagaagca catgcaatgc tagatcatat tcacatgctt gttcgagtac caccaaaaat
      121 atgtatatcc agttttatgg ggtatctgaa aggtcgtagt gcggtactaa tccacgagca
      181 gcacgcaaat ctcaaatata aatacggaaa caaatccttt tggtcgaaga gatactatgt
      241 gagtacagtc ggattgaatc aaaagacaaa tgaaaaatac atccgtgaac aagaagctga
      301 tgatcgcatt cgatatatta taagtaaaag agagtacgta gaaccattta aaaaataaag
      361 agaagtaagt gagtgaagat gagcagttgg cggtctttta aaaatcccct tttaaggggc
      421 agcgagtgtc gaccccttct agggggatta aaaatacacc cgtcctcacg ggtgtatggg
      481 ttactgatag acttgatttt aactggtgtc agaaacttat gtccagaaag ggtgatgaaa
      541 ttttggcaat ttacggctat tcaagaatta gcacaagcaa ccaagattac aaaacacaaa
      601 ttcaaacatt agaaaatgct ggcgcagaaa taatcttcct agaaaatcac tggattatcg
      661 aacacctttg gaagtatttt tgagttacgt aagtattgat gatctgtcta acttaatttg
      721 acaattaaga gtatatttac ttcagatggt aaggcaacaa aataaaatac aataggcaga
      781 tgaactaata aataaaatct gtattaaata cagataaatt aatataaaaa tcaaaaggat
      841 cataaacttt taacaagtga tgaaatgggt ggaaattgat tcttatagac agtttgtctc
      901 ataacattga agtattttgc aattttagca ttgaaacact tttttaatct tcaacgttgt
      961 tcttataaac aagatgattg ggaatctttt gaattaacac tgtatagctt agaacaccct
     1021 ttatagaaaa cattcgctta gcaatcttaa taattccttg tgcccgatgt cgtttagatt
     1081 cagtaggttc ctgctcatcg atcatggaaa aaatttgaag gatcagatcc ttaataaatt
     1141 tatttaagag aggatgatcc attgcttcat ccatactcga taaactagtg ataatgagct
     1201 acacgttttt ctttcaagtt gatcaatagc ttccacgata aaaatatctt tagataaaat
     1261 caagaaccgt aggaactaca gggatatttg gtcaataaga gccaccgcta ttagtaaaaa
     1321 ataagctatc aagtatgctc tcatcccaag aattgcctac ttcaatcgtt aaaaaccgtt
     1381 aggtttagct aagtgggggt agttcactca atacttttat caataataga gcgcaaaact
     1441 ttttctctta attca
//
LOCUS       DAFCNS010000178         1417 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFCNS010000178 DAFCNS010000000
VERSION     DAFCNS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.190"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="LNE23_002865"
     CDS             complement(150..1337)
                     /locus_tag="LNE23_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBK6932661.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAFCNS010000179         1373 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFCNS010000179 DAFCNS010000000
VERSION     DAFCNS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.191"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            191..445
                     /locus_tag="LNE23_002866"
     CDS             191..445
                     /locus_tag="LNE23_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6932662.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="LNE23_002867"
     CDS             446..763
                     /locus_tag="LNE23_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6932663.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          492 a          196 c          268 g          417 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DAFCNS010000180         1364 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFCNS010000180 DAFCNS010000000
VERSION     DAFCNS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.192"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>1364
                     /locus_tag="LNE23_002868"
     CDS             82..>1364
                     /locus_tag="LNE23_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBK6932664.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAFCNS010000181         1342 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFCNS010000181 DAFCNS010000000
VERSION     DAFCNS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.193"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            161..898
                     /gene="erm(B)"
                     /locus_tag="LNE23_002869"
     CDS             161..898
                     /gene="erm(B)"
                     /locus_tag="LNE23_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBK6932665.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1068..1328
                     /locus_tag="LNE23_002870"
                     /pseudo
     CDS             1068..1328
                     /locus_tag="LNE23_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          514 a          222 c          219 g          387 t
ORIGIN      
        1 aaacagacta aaaacagtga ttacgcagat aaataaatac gttagattaa ttcctaccag
       61 tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc gtttaacttc
      121 tgtatttgtt tatagatgta atcacttcag gagagattac atgaacaaaa atataaaata
      181 ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac aattgaattt
      241 aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa cgacgaaact
      301 ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat tcaacttatc
      361 gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata ttctacagtt
      421 tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc atttaagcac
      481 acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct gacatctatc tgattgttga
      541 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
      601 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa
      661 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
      721 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
      781 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccattac
      841 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt
      901 ctatgagtcg cttttttaaa tttggaaagt tacacgttac taaagggaat ggagataaat
      961 tattagatat actactgaca gcttccaaga agctaaagag gtccctagcg cctacgggga
     1021 atttgtatcg ataaggggta caaattccca ctaagcgctc gggacccctt gtaggaaaat
     1081 gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt ttatggttgt tcaaattatc
     1141 ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa aaaactaacc aaaacgaatg
     1201 taaaagaatt actggaggga aaagaaaccc tggtaaaagg aatcaaaaac aaagagaaaa
     1261 agccctacaa tgccgttgta aaaattgggg aaaagggata tattgatttt atctcttttt
     1321 caaaataaac ataaaagccc tt
//
LOCUS       DAFCNS010000182         1325 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFCNS010000182 DAFCNS010000000
VERSION     DAFCNS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.194"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(315..398)
                     /locus_tag="LNE23_002871"
     tRNA            complement(315..398)
                     /locus_tag="LNE23_002871"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..>1325
                     /locus_tag="LNE23_002872"
     CDS             926..>1325
                     /locus_tag="LNE23_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6932666.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSV"
BASE COUNT          402 a          235 c          207 g          481 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaa
//
LOCUS       DAFCNS010000183         1313 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFCNS010000183 DAFCNS010000000
VERSION     DAFCNS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.195"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="LNE23_002873"
     CDS             200..742
                     /locus_tag="LNE23_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932667.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1272
                     /locus_tag="LNE23_002874"
     CDS             898..1272
                     /locus_tag="LNE23_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBK6932668.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDVEWQHFF"
BASE COUNT          532 a          148 c          256 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgt ggaatggcaa
     1261 cacttttttt gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gct
//
LOCUS       DAFCNS010000184         1272 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFCNS010000184 DAFCNS010000000
VERSION     DAFCNS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.197"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..858)
                     /locus_tag="LNE23_002875"
     CDS             complement(<1..858)
                     /locus_tag="LNE23_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK6932669.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKNR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKAC"
     gene            complement(836..1150)
                     /locus_tag="LNE23_002876"
     CDS             complement(836..1150)
                     /locus_tag="LNE23_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBK6932670.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
BASE COUNT          373 a          263 c          178 g          458 t
ORIGIN      
        1 acaagctttt tcccacatgt cgggatcatc aacttcatta tcgttatcca agcgacaaat
       61 aaatggaaac aaactagaaa attctgctcc atccttgtca attccaagtt ctccagaaag
      121 aatcattttt gattcttcta taatgtcatc aagcggacca ccacgaacat gaccattagt
      181 tgtatcataa aattctctat aatctcgatt tttaccacca ccagaagtag ccacatttat
      241 cattgaataa tcttcatttt cgtgaatttc atcaaagcgg tttgcacctg gtcgcttccc
      301 atcttttgtt ctagcatttg ccgtgttata acgaagtttg ctgtttgtag cgatattttg
      361 aataacttcc ttcgtagctt taaatacttt tttatctaaa tcaggatgat ctttaattac
      421 tttaaataca tcatcaaaac tagtctttgc ttggctttca ttattggcat agatatcaat
      481 atcataattt ttaataccgt gtttagcggt tagtagaaag aagttgttcc aagaagcaaa
      541 accagtttta ccattaccac gtcccattaa tgaaagatat ctattgaaca ctagtgtttt
      601 atcttttttc catcgaacac cataaataaa acattgtaga aatttttccc acggaattaa
      661 ttcgaatgga aagtattgtg ctggtatatt gattgaatcc tctaccatct gcttatcgaa
      721 gtaaatatct tctctagtaa agactctttc ttctagataa ttttttagca ataattgctc
      781 tttgcatacc ttgatagtgc cttcttctat agctttgaac caattttcaa tatgcttata
      841 actcaggaat tgattcattt gcttcaccta ccaattcagg agtaatggca agtttatcca
      901 acatcaatcc catttgtttg ttgacagaaa caagcaacgc tactgattca ttctttttac
      961 cattctccag tctaatgccg ttctcggata tatcttcttc cagtgatatc gccgtttccc
     1021 ataaactgat ataacgatca acattatcta agaatggctc aatatttgtt ttctgacttt
     1081 ccaattggct tattaaagag cggcgtaatt tttctctgta gcgattttga gacaattcgt
     1141 ttttaaacat tttagccctc ctttcatgat aaagttcgaa aaaatctctt ttcctgacag
     1201 ccccctccgt ttcatcaccc ccaaaaaatt tgcgatttat tttaaggggg ggttatctca
     1261 ccatcggaat ga
//
LOCUS       DAFCNS010000185         1264 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFCNS010000185 DAFCNS010000000
VERSION     DAFCNS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.198"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..1203)
                     /locus_tag="LNE23_002877"
     CDS             complement(31..1203)
                     /locus_tag="LNE23_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HBK6932671.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          343 a          263 c          243 g          415 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttac
//
LOCUS       DAFCNS010000186         1215 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFCNS010000186 DAFCNS010000000
VERSION     DAFCNS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.199"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..>1215
                     /locus_tag="LNE23_002878"
     rRNA            2..>1215
                     /locus_tag="LNE23_002878"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          368 a          242 c          352 g          253 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 tatggtagtt ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac
      361 cccgtagacg aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa
      421 ttccgtcgga atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt
      481 gaaccagtac cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg
      541 aaaccgtgtg cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga
      601 atgaaccggc gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa
      661 agcgagtctg aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc
      721 catgtccagg ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa
      781 agtgcgggga tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt
      841 ctctccgaaa tagctttagg gctagcctcg gaattgagaa tgatggaggt agagcactgt
      901 ttggactagg ggcccatctc gggttaccga attcagataa actccgaatg ccattcattc
      961 atatccggga gtcagactgt gagtgataag atccatagtc gaaagggaaa cagcccagac
     1021 caccagctaa ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa
     1081 ctaggatgtt ggcttagaag cagccaccat ttaaagagtg cgtaatagct cactagtcga
     1141 gtgaccctgc gccgaaaatg taccggggct aaacatatta ccgaagctgt ggagtacacc
     1201 tttaggtgta ttggt
//
LOCUS       DAFCNS010000187         1210 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFCNS010000187 DAFCNS010000000
VERSION     DAFCNS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.201"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            40..591
                     /locus_tag="LNE23_002879"
     CDS             40..591
                     /locus_tag="LNE23_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HBK6932672.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            886..1059
                     /locus_tag="LNE23_002880"
     CDS             886..1059
                     /locus_tag="LNE23_002880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932673.1"
                     /translation="MSEKTKKIIRVSLAFSQVVVQSLIVYYTYQQYKWFCCKVFQKIY
                     FSVKLRKKTERTE"
     gene            1059..>1210
                     /locus_tag="LNE23_002881"
     CDS             1059..>1210
                     /locus_tag="LNE23_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBK6932674.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          455 a          165 c          234 g          356 t
ORIGIN      
        1 aagagcgagc aagacaggaa cgagagaaca aacatactca tgaaaagact agaggcttcg
       61 acctcgaact ttaataataa gttagatgtt tttgaagaaa agactaagca tgcaagatta
      121 aactttgaaa caactgcaaa gcactatatt aaacgacttg atgaagataa tttgaaatta
      181 gattttcaac aagctattca agatgaatta agcgatacta aagacgaaat acgtgaagtg
      241 acgaaacaag cgagagaaga aacaaaagag tataaagaaa tcttagaaag taaaataaaa
      301 gaccataaca aagtggttga taaatcgaat acagcgctaa aagtgatgac aaaaggcgtt
      361 acaaatatat tttttgtgct aattattttt gtacttgtaa tgcttgtgac aggtccgata
      421 ggtcatttct ttggtattga acatttatat agctttatta atggttttat tgatgaccac
      481 gaaagtgcat ggcgatattt gatgttgata ctttatgcag taccctatgt attcttcgct
      541 atcattctgt ggctcatagc attgtttttt agagtattta atgaccttta ggtagattta
      601 tatataaaaa gggctaaata cgcaaaatac ccccttaaaa tgcaaaataa atgcaaataa
      661 gggggtaact ccttatgaaa aaacaactaa tagtattata acacgttttt atgccgagaa
      721 aacttattgg ttggaatggg ctatgtgtta gctaacttgt tagcgagttg gttggacttg
      781 aattgggatt aatcccaaga aagtaccaac tcaacaacac ataaagccct gtaggttccg
      841 accaataagg aaattggaat aaagcaacga aaggagttga gtaaaatgag tgagaaaacg
      901 aagaaaatca ttcgagtatc attagctttt tcacaagtag tagtgcaatc attgatagtt
      961 tattatactt accagcaata taaatggttc tgttgcaaag ttttccaaaa aatctatttt
     1021 agtgtaaaat tgagaaaaaa gacagagagg acagagtaat gaatcatttt aaaggcaaac
     1081 aattcaaaaa agacgtcatt attgtcgctg ttggttacta cctgcgttac aatctaagct
     1141 atcgtgaagt tcaggaattg ttatatgatc gtggaataaa tgtttgtcat actacgattt
     1201 atcgttgggt
//
LOCUS       DAFCNS010000188         1159 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFCNS010000188 DAFCNS010000000
VERSION     DAFCNS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.202"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            14..1141
                     /locus_tag="LNE23_002882"
     CDS             14..1141
                     /locus_tag="LNE23_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6932675.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          418 a          200 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaa
//
LOCUS       DAFCNS010000189         1151 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFCNS010000189 DAFCNS010000000
VERSION     DAFCNS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.203"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..253
                     /locus_tag="LNE23_002883"
     CDS             <1..253
                     /locus_tag="LNE23_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6932676.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            341..742
                     /locus_tag="LNE23_002884"
                     /pseudo
     CDS             341..742
                     /locus_tag="LNE23_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            991..>1151
                     /locus_tag="LNE23_002885"
     CDS             991..>1151
                     /locus_tag="LNE23_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6932677.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          405 a          183 c          223 g          340 t
ORIGIN      
        1 tacggaaggc tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa
       61 aaagaaattc acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgcc
      121 ccactcaaac ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc
      181 gtttggttat cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat
      241 ctttgaaaac taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
      301 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct acaaatatta atagtggaga
      361 attaaataaa gatggctatg tgcaggtggc tagggagatt cacaagaagt ttggatgtag
      421 gtatgtagct acaactctgc gtagaagtta ttcggcaagt agaaatggtt ggagtgcaat
      481 gttatacgaa gcagaaaaag ataaagcgta tttttccaca gaatacaaca tacaaattgt
      541 tgatcgcgtt ggtggtggag acagcttttc tgctggtata atatatgcgt tatgtgaaaa
      601 tatgtccgga caaaaggcta ttgaatttgc gactgcatct agttgtttaa aacatactat
      661 agaaggtgat tttaatcgaa ctacaattga ggatgtatat aatttaatga atagcggtgg
      721 aaatggtcgc gtgttgcgtt agtcaacata taaagataaa tcacagtaat ttttatggtg
      781 attatctgtc ttagtagtga ttagaataac gtgaaaataa attgttgagc gtttatactg
      841 ttttgaaaaa taaatttaac atattgaaaa atacaaatat atacgtatat tataaagcgc
      901 cgattgtaaa attaagctag acaactaaaa cagtcatttg tgctacactc aaaatagttc
      961 cgaccaaaga attataagga gtgagtgcaa ataacctata cctatcttac aacggatgaa
     1021 cttgtaataa tagagtctta tttgaaaatt aaccaatctg ttgctaaaac tgcgcattgc
     1081 ttgaatcgtt caagtcaaac gatccataaa gtatatctgt tcttcaagca aagaaaatca
     1141 gccttagaat a
//
LOCUS       DAFCNS010000190         1110 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFCNS010000190 DAFCNS010000000
VERSION     DAFCNS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.206"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..234)
                     /locus_tag="LNE23_002886"
     tRNA            complement(161..234)
                     /locus_tag="LNE23_002886"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ile,seq:cat)
     gene            complement(789..1103)
                     /locus_tag="LNE23_002887"
     CDS             complement(789..1103)
                     /locus_tag="LNE23_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932678.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          418 a          174 c          170 g          348 t
ORIGIN      
        1 tcaaatatac agaagtataa ctatgtgaaa catcctttca ttaatccata aaaggataca
       61 taaaaaagcc actcatttga gtggcaatga agaaaagctt tagcttgtat aatactcttc
      121 aaaaaattct aacacagaac gattcaattg gctacattaa tgtaccctgt aggactcgaa
      181 cctacgaccg gacggttatg agccgtctgc tctgaccaac tgagctaagg gtagtggttg
      241 ttgccacata aagccataaa caatcaacca gtagaatgcg tggcaacaaa cctgttatcg
      301 catatcttgg agtgtgacta tttatgagtg atagtgaaga tatgcgtctc catatatgta
      361 tggttgagac agataaacat gtatgaaaaa cgttgttaaa tcaacgtttt tttattttgg
      421 tcaaggagag aaaagtagag aaatacaaaa ttttttgcat tgattttgca ttcaaaaaaa
      481 cattcaaaca tttagaaatc cccaatacgt attcaggaaa atctatttta aggtttacaa
      541 aaagaacttt tgttcgtaca ctctttttga ggtgaacttt tatgctgatg gaaggaaaaa
      601 cacaattatg gtttaagcat gttccttcaa ataagttttt taattataaa ctctagagaa
      661 attaattcat cctaacataa aataattgtt cactcaaaag tttttctaga acaattaaaa
      721 cagcaggctt cacattcgct atacatttgt aagaagtaat aattcctgta ctaaaaggca
      781 taattgcttt aattattttt tgacttctct tcaaacacaa gaagtagttg tgtaataatt
      841 gcagaaataa ggattccaat aatagatata aaatttgaca tggaaacgtc tataacatta
      901 aaagaataaa gtgttgaaga aagtaaactg attgaaataa cccctccgta aaaaatagtc
      961 tgtgaatata cccacttttt ttcagaagac gtacttcgtt tggttctaaa acctatccct
     1021 gcatttctac tcttatcaaa aaaccaaata tttagaagta ataatattgc tattattaag
     1081 ataaaattac tgtaaaaatc caaaagctat
//
LOCUS       DAFCNS010000191         1096 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFCNS010000191 DAFCNS010000000
VERSION     DAFCNS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.207"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..404
                     /locus_tag="LNE23_002888"
     CDS             <1..404
                     /locus_tag="LNE23_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HBK6932679.1"
                     /translation="EWVADFAEVQDKILKFAPTMPVEKTEFYVELFREKLAGLLVPTS
                     SGHGSVDLILSECHKTFGLKMLVERLGINPDQCVAFGDGGNDIEMLEYCGLSYAMDNA
                     TEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(477..836)
                     /locus_tag="LNE23_002889"
     CDS             complement(477..836)
                     /locus_tag="LNE23_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK6932680.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          354 a          189 c          187 g          366 t
ORIGIN      
        1 tagaatgggt agctgatttt gcagaagttc aagataaaat tttaaaattt gctccgacta
       61 tgcctgttga aaaaaccgaa ttttacgttg aattgttccg tgaaaaatta gccgggctat
      121 tagtacctac cagtagtggt catggttctg tagacttgat tctttctgag tgccataaaa
      181 ctttcggctt gaaaatgtta gttgagcgct tggggattaa tcctgaccag tgcgttgctt
      241 ttggagatgg gggcaatgac attgagatgt tggagtattg tggtctgagt tatgcaatgg
      301 ataatgcaac ggaagctgtt aagcaagttg ctaagcatca atgtccttca aatgacgaag
      361 acggtgtttt agtgacgctc gatcgattgt tccctaacac gtaaacacta ggtaattcat
      421 caaaaaacct cgatataaga aatattcgag gttttaaaac tatatttaga acagatttac
      481 gaagcgtctg atgagtcaaa atagacttgg acattgctat ctaaaatacc actttcattc
      541 tttaaacgaa ttgtttgatt tgatggagtt acctctaata ctaaatgccc attaataaag
      601 gtagtatcgt attcagatgt tttaactgtt gcttcttcgc aaggtattgt taaatcatat
      661 gctggaatat aatgttgtgt ggaaactaat aaaaaatgat caccaatcat acagcatccc
      721 tctgctgaag aaaattgatt agagccatca tttaaagtta acaagaatcc atcctttaaa
      781 cgaggtactt gcttaaatac ttctggttgt acttctaaaa ataaattatc gctcattttc
      841 acgaccacca ttgacttcca cagttgaaat tgacatttta tttaacacac ctattccccc
      901 ttaactataa ctcaattcta tataaaaaat attctattct caaataattg aacttttcaa
      961 atcgccaatt tacttcggct ttatcagagc tcattcatat caagagcgat taacgctgta
     1021 aaaattaaac cgaagatctg ataaattttc tctattttct acaatattta aaacgcttcc
     1081 ttagtctatt atatat
//
LOCUS       DAFCNS010000192         1060 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFCNS010000192 DAFCNS010000000
VERSION     DAFCNS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.208"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(38..994)
                     /locus_tag="LNE23_002890"
     CDS             complement(38..994)
                     /locus_tag="LNE23_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932681.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt
//
LOCUS       DAFCNS010000193         1051 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFCNS010000193 DAFCNS010000000
VERSION     DAFCNS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.209"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="LNE23_002891"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="LNE23_002891"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBK6932682.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAFCNS010000194         1045 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFCNS010000194 DAFCNS010000000
VERSION     DAFCNS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.210"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            329..973
                     /locus_tag="LNE23_002892"
                     /pseudo
     CDS             329..973
                     /locus_tag="LNE23_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT          358 a          118 c          227 g          342 t
ORIGIN      
        1 tttaaaaatt tttcatttta cagtaagtat ataaataaac ttgcgagaac cagtttaggg
       61 gtatatattt tacatgaaaa tcattatttc attgatggta tgaagtctag aggcttaata
      121 tgggatggga tatttggatt aggcaaaagg tttttagata gggatttttg gatttggttt
      181 ataattgcag ttgtagttgt gtgcataatt tgcatggcta tcgatgttgt aagagaaaaa
      241 tttgaaagac agatttttaa aattaagagt gtaaagaacc tgtgtaaact tatgaatcat
      301 gttataaata tatacccata attaataaat gaggagaaaa gaagtgaaag ttatatcatt
      361 cggagaatta ttattgagac ttgcagcacc cggttattca aagttatttc aaagagatag
      421 tttggagact acattctgcg gaggggaagc taatgtagcg gtatccttag caatatttgg
      481 atatcaatca gaatttataa caaagcttcc ggattcagat gttggatatg cggcagctaa
      541 tgctttgcgt tattttggag taaatgttga taatattata tttggagagg gacgcatggg
      601 actgtattat ttagaaaaag gagcagctca gagaccatct aaagtaattt atgacagaaa
      661 attttctgca atttcgttag ctgatagtga tgaatttaag tggaaggaga tcctaaacga
      721 tgcaaattgg tttcattgga cagggataaa tccagcattg tctgagaatc tctttgagac
      781 gtccttagat gcatgtcagg ctgccaaaaa aagaggtata acaatttctt gtgatttaaa
      841 ttatcgatcc aagctttgga catcagagga agcaaaagaa aagatggaac agatactacc
      901 attggttgat atttgtattt gtaatgaaga agatgcagaa aaagtgctag gaattaaagc
      961 tgagcataca aataggttct ataatatttt gtgttggtgg aaaatcgaaa gcgattttct
     1021 actaacactt ttttcttcgt ttcaa
//
LOCUS       DAFCNS010000195         1024 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFCNS010000195 DAFCNS010000000
VERSION     DAFCNS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.211"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..>1024)
                     /gene="menA"
                     /locus_tag="LNE23_002893"
     CDS             complement(132..>1024)
                     /gene="menA"
                     /locus_tag="LNE23_002893"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBK6932683.1"
                     /translation="FAVGVLFSIAFFHEIHWLNTFLFFIGMLVFDLATTAINNYMDYE
                     KAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIGVILTINTGWLLLLMGFICC
                     FIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVNTFGSDVYDLTVNFSSGQFD
                     LVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIENHRYTLVFYIGRPAGTVLF
                     QLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLDQFKQTLPQPISFSYSIKNM
                     VLFNASYVLGLLLTIIMQAI"
BASE COUNT          377 a          205 c          177 g          265 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaag
//
LOCUS       DAFCNS010000196         1001 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFCNS010000196 DAFCNS010000000
VERSION     DAFCNS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.212"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>1001)
                     /locus_tag="LNE23_002894"
     rRNA            complement(<1..>1001)
                     /locus_tag="LNE23_002894"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          210 a          311 c          227 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc g
//
LOCUS       DAFCNS010000197          998 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFCNS010000197 DAFCNS010000000
VERSION     DAFCNS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.213"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(80..634)
                     /locus_tag="LNE23_002895"
     CDS             complement(80..634)
                     /locus_tag="LNE23_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6932684.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          323 a          185 c          107 g          383 t
ORIGIN      
        1 ttccgaaaat tcagttatct tcattaatct caccttctaa gttttcatta tacacaaaaa
       61 atatatccta atatctcaat tagcctccca atttaaaacc acgtaacgat gcccctattt
      121 catcttcagt tataccaatg tacctttttg taattgcttg cgagctatga ttaaaaataa
      181 acattaaggt agcaatgtct ctagttttct tgtagtagtg atacccaaaa gtttttctta
      241 atgtatgtgt tcctatatca tctcttccta atttttctgc tacatctgca tatatctgat
      301 agacacgatc ccttgttata tgtcgacctt gttgccgact tggaaataac caatcatttt
      361 cttctttacc ttcagtatat aataaaatct cttctctcaa attatctata aaaatcggtt
      421 ttcgcttacc tgttttatgt tcaacaataa taggcttact tttacctcgt atgtctccaa
      481 cttttaatcc tataatatct gacatacgta acccattatt gattccaaac aaaaataaaa
      541 aactatctct ttctggatta cttgttcttc ttagccaaaa ctgaaaatct tgtatctctt
      601 catttgtcct taacggttgt acattatagc tcactattgt ttcccttctt aaaaatattc
      661 tgagttcatg attatatata tattttacca taatcatgaa cttatatata tactctgtta
      721 aaagtgaaga aaatcaaggg gtttagttta tgattttata tcatttcagt aactcagaat
      781 attccgactt ttttgtacaa aaaaattaac cttacttata tgaagattct aagctcttat
      841 ttaaatttta gacgggcagt tatccgtaga agttcttaga atagctaatt tacataatta
      901 aattatatga aatttaagtt tgaacaatat actaagaact ccccttacta taaagtgaaa
      961 taaattagca tatgaataac cgtgctacca aacttttc
//
LOCUS       DAFCNS010000198          926 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFCNS010000198 DAFCNS010000000
VERSION     DAFCNS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.214"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..716)
                     /locus_tag="LNE23_002896"
     CDS             complement(<1..716)
                     /locus_tag="LNE23_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6932685.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLY"
BASE COUNT          316 a          141 c          122 g          347 t
ORIGIN      
        1 atataaagtt gtgatatttt tccagctaaa gatccaccat taagaaattc ttcaatggct
       61 gtttgtaaaa ctaagtctgc agactttcct ataggtgaat gaatatagtt agttatagca
      121 ataatttttg cgccattttt tttagctatt ttcaatgaat cataagtatc tttcgttttc
      181 ccagataaag aaaaaatgat tactaaatca ttaactgtta ataaagaagc gacttgggct
      241 tgaaaatgag ggtctagtac agcttttgct tgaactccta cgcgaagaaa catattttct
      301 aaatcaagac tagtatttcc actagatccc actccaaata tatatatgtt ttttgattga
      361 gtgatcattt taatggcttc attcaatttt tcttcgtcta aaagcctaat agtggaatag
      421 agagcttctg ttaaactatt agcaatttca tcatagtact ttacactagt cggatgttct
      481 tttttttgac taaagtcttc ctttgcaatt tcaattttta aatcagaaaa accagaaaaa
      541 ccaatttttt gacaaaagcg aataatagta gcatcgccaa cttttgtgtt tttcttaata
      601 tcactcatcg tactataaat aatagttttc ccagaattta aaataaaatc agcgactttt
      661 ttttcagact tcgttaaaaa tggataattt tctttaatta aatagatata actcatacaa
      721 actcctctac aatttcaact tttctccatt atactccaaa ataataaaaa aaactcctaa
      781 atactccatt tttatcttta cttttatgaa agcggatgct atattgtttt tggagttgtt
      841 atccaaaaat aataaaaaat tggagtgttt aggataatgg ataaaaagag gtttttagat
      901 cttatttcaa aaggtttgat tgtttc
//
LOCUS       DAFCNS010000199          903 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFCNS010000199 DAFCNS010000000
VERSION     DAFCNS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.215"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          349 a          103 c          113 g          338 t
ORIGIN      
        1 attagcaata tataaaagaa ggtatcaaga gtcacagtga atttattccg aaaagtgaaa
       61 agagcaaata taaaatttaa taaaaataag aactaaatag gaaaacgtag acgtaatttt
      121 ctacgttttc cttttcatat atattaaata ttacggaaaa ttatataaac taatttagtg
      181 ttttgtttgt aattcctaat tttgctccac ttttagataa tttttaggtc tatttaactc
      241 attagacatt ctatgctcat ttttctccaa atttgtttca actctttcgt acttaatttt
      301 gcgtatttga aaattcttct tttagagtac ttttattacg atactagtat gatatatatc
      361 tacagcaaac agttaaaatc ttcctaaaat aagcgaatag cattcctaaa acctataaaa
      421 tttttagatg aagcgacaag tggacaatta ataaactact tgaacatgaa attatggatc
      481 acctttcaaa tttagaaaat ggaattctta tacttattgc gcaacattta ttgattgcaa
      541 aattatgtga caaaaagaac tagtagaaga aggaataatg gcttgataat aaagtactac
      601 ctattaaagg tagtacttta tttaatttag tataaattaa catactacta tattttaggt
      661 ttcatattat ataaattaat aatatactac catttgttgg tagtataatt taaaggtaat
      721 aagttattct tttattacta tgtttgggtt ttatagcaaa gaaagtttaa taagagtttg
      781 gacctagcaa acaatattaa tgaggtcatt aaatcttatt atctttagtg atataatgaa
      841 atatctacac gacaaaatta tgatcttgcg taaagttata aaatattaat aaaaatattt
      901 tat
//
LOCUS       DAFCNS010000200          901 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFCNS010000200 DAFCNS010000000
VERSION     DAFCNS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.216"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(124..600)
                     /locus_tag="LNE23_002897"
     CDS             complement(124..600)
                     /locus_tag="LNE23_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6932686.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(613..876)
                     /locus_tag="LNE23_002898"
     CDS             complement(613..876)
                     /locus_tag="LNE23_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HBK6932687.1"
                     /translation="MNKNTSLHEESLIIIEALGGKENIESVEACATRLRVAVTNGEII
                     QKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
BASE COUNT          298 a          167 c          118 g          318 t
ORIGIN      
        1 catggggaat cattactaat aaaataaaaa aatgaacaga aagaaaacag ttctttctgt
       61 tcaccaactt catttgacaa atatcctatt tagcttattt tatagctttt tatatacact
      121 tttttaaaaa gtgatttttc cacatgaatt cccatgagca attcgaccat tatctattat
      181 taaatcattg aactgatttt tctccggaaa aacaactata acagaagtat ctttaccaga
      241 ttttattatt ttgtcaaaat ccacatcaac caacgaggtt tcaggagtaa cctcatcatt
      301 ttcttgaaca caaatcgaaa aaggctctcc ttgtaattca actgtatcaa ttccaatatg
      361 aatcaaataa tcaatgcctg agttgctttt tatattaata gcatgtttag taggaaatat
      421 atttgttact cgaccagcca atggagcata aatttttgaa ccatcaggta tgacggcaaa
      481 accatcacct agtaattttt gtgaaaagac aggatcattt acttctgtga tggaaatgat
      541 ttttccggaa caaggtgcat aaaaagtttc ttgttttttc tttttgaaaa tttcaaacat
      601 attaaaaacc tcctaattat cttttcctaa tatttgattg atttcttgac ttaataagtc
      661 agctttccct ccaaaaacag cttggatgcc acccttcact tcgaagacag cagttgcacc
      721 agtgtctttt attgtttgtt tttgaataat ctccccattc gtaacagcaa cacgcaaacg
      781 agttgcacaa gcttcaacag actcaatatt ttcttttcca ccaagagctt caataatgat
      841 aagagactct tcatgtaatg atgtattttt gtttattact ttttcagaat gaacttctga
      901 t
//
LOCUS       DAFCNS010000201          898 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFCNS010000201 DAFCNS010000000
VERSION     DAFCNS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.217"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>898)
                     /locus_tag="LNE23_002899"
     CDS             complement(<1..>898)
                     /locus_tag="LNE23_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6932688.1"
                     /translation="LKDFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASE
                     ANIRSFFYYFYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWL
                     AINIERSSFRKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFG
                     VIDGNAIYENSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHE
                     RSYLFYGNPDLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQLF
                     LDYYYVLDYYDLFLTNVKPIKIL"
BASE COUNT          325 a          147 c          112 g          314 t
ORIGIN      
        1 taaaatctta atcggtttaa catttgttaa aaataaatca taataatcta acacataata
       61 ataatccaaa aataattgtg cccagttttc taatttcttt gatacatcca aatccgcatt
      121 tgcttgtaat tcttttttga gtttttccat tatgtgataa gttcgaggtt cttcttcttt
      181 catttcttcg atatacgaac gattaaaaaa aagatctgga ttaccataaa atagataact
      241 tctttcatga aatgcaataa aattgaatat gagttctgga tcctttatat ctaaagaaaa
      301 acggaacatg ctttgtaaaa ctttctccaa attctcaact gctcgataag aacaagtatt
      361 ttgtcgctga tgtgcatatg catgactatt ttcatatatt gcatttccat caattactcc
      421 aaaagaataa attattcgat aaaggaaaca cagttcatcg ctgttaaaag aaagattgat
      481 ttgttttgac cacttcttca aaagattaaa atgcggatca tctgagataa ctgatttata
      541 ttcattatct actcgtactt ttctaaatga acttctttct atattaatgg ctaaccaaaa
      601 agaaaataca cgtttttgaa aaatagttaa cgtaatatca taaatcccct caaaagcaac
      661 tatatacttc tctatttcct tttgttcgat atgaaatggc cacttatcac tactttcacg
      721 tgtagaattc caatagaaat aataaaaaaa gctacgaatg tttgcctctg atgcattcaa
      781 ttctaactga aatggatgaa atgttatatg ataacgccta aaatatggaa ttaagagctt
      841 cgcatatctg aaaacagtag atacactagt aaattgctct ttagcaaaat cttttaga
//
LOCUS       DAFCNS010000202          894 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFCNS010000202 DAFCNS010000000
VERSION     DAFCNS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.218"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            253..876
                     /locus_tag="LNE23_002900"
     CDS             253..876
                     /locus_tag="LNE23_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6932689.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          329 a          129 c          167 g          269 t
ORIGIN      
        1 aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac ttaaaggagg
       61 tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt tgttgtttct
      121 tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt tttcagcggt
      181 ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata gattggatag
      241 gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca aagtttagct
      301 cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga agacgattta
      361 aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga gctgcaagag
      421 cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga tcgattagca
      481 agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg tgcttctatt
      541 gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag agacttgcta
      601 acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa aaaaatcaaa
      661 gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac tggtaaaaag
      721 attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga aattgttgat
      781 ggtctgaaga aaaaagaaac tatttctaaa atagcaaaga acgcaggagt tacaagaaaa
      841 accgtttatc gagtaaaaag agatttagga atgtaatatg ttccagttag aaag
//
LOCUS       DAFCNS010000203          864 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFCNS010000203 DAFCNS010000000
VERSION     DAFCNS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.220"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..638)
                     /locus_tag="LNE23_002901"
     CDS             complement(<1..638)
                     /locus_tag="LNE23_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6932690.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSN"
BASE COUNT          267 a          168 c          142 g          287 t
ORIGIN      
        1 ttgttggacc gatctaaaat atctccgtcc gattgctctt cgtagcaaag catcaaatca
       61 ctatcaatga ctacatcagt tcctgctact aagccttcga aactaaacac ataatcattt
      121 aagatgaact ggcatgaagt agctgaagga gtgatgataa tctttggaaa actttcttct
      181 aaactattat tcagcaagtt aaatgactgt ggtttatcta cggttatagg tacatcttct
      241 tgaactcttg cgaatggttt cgcagtaata tttacatcga actctcccca ttcaacaata
      301 tcgttttctg catccccaat atcgatagtc tggataacgt aatagacgtt gggatcgtca
      361 gagaattcta atttctttgc atagtttaac caatgacgca tgatataaaa cgattgcttg
      421 aacgcttgat ggtcttccac atcctctaaa tagttatagt gcaatgtaaa cgacatatct
      481 tcaaacgagt aatcttgtac taagccacct aacctaccta aaacagaagt ttcaactctc
      541 tgtctttttg gagaaggtat ggttggtctt tcagctaaag ccaatttatg caaataatca
      601 ggaaatccat cgattataga atgtatacaa tcagtcattt tttcacatcc tttttaatac
      661 taaaaaaaca ggagaaatac tctcctgttt aacgccatgc cgaagcatta tcattttgaa
      721 tttttgtaat gctatcaatg atttgttgag ttgtttgctt catagtaacc tcatctgcgt
      781 taccatcaat tgtgaaattg aattcgtagt tattcacagg ttgaatcgtt tgtgccctag
      841 atgaaactga tgtgctactc aaga
//
LOCUS       DAFCNS010000204          843 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFCNS010000204 DAFCNS010000000
VERSION     DAFCNS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.221"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..794
                     /locus_tag="LNE23_002902"
     CDS             <1..794
                     /locus_tag="LNE23_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK6932691.1"
                     /translation="EYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVASYDDVLHTKEKE
                     FPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALRLQNIKREVIDI
                     SIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILKPALEEAGFTVE
                     IVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISNGNIRYEKIEPE
                     TRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
BASE COUNT          290 a          116 c          173 g          264 t
ORIGIN      
        1 aagaatactc tcaaatgcaa cgtaatgctg gtttaagact tacgttcatg accaaacgaa
       61 tgaacagacc tatggaagat acacgatttg ctgttgcttc atatgatgat gttctgcata
      121 cgaaagaaaa agaatttcct gaaaaaatgg atgaagtgat aggaacagtc gattttgctg
      181 atagacgaga ttttgccagc gttgggttgc taggaaaata cgataaagat gtttatttta
      241 cacaacatac ttttatccac gaatcagccc ttcgattaca aaacatcaaa cgagaggtta
      301 tagatatttc tatagatcaa ggaaaatcac agatcgttca tggaaaaaat atagaagctg
      361 attatattgt aggttggttt cttgaaatga gtaataaata ttatattaaa aaaatcgcta
      421 tggatatgta ccgtgcaaaa atattgaagc ccgctttaga agaagcaggt tttactgtgg
      481 aaattgttcg aagcggatct gttacacatg gtatgttaaa agatctggtt gatgaccttt
      541 ttattaatca acgtttattt tttggtgacg atgcgattat gcgttggtat tgcatgaatg
      601 tatatgaaga gcatatttct aatggaaata tacgctatga aaaaatagaa cctgaaacta
      661 gaaaaacgga tggctttttt tcattccttc atggtttgaa ttttttagat gatatttatg
      721 attctgctcc tgtaacagtc acaaatagct cagtagaaaa tacaggaact ggatttactc
      781 ctctagtatt ctaacttgaa aggaggtgag aaagtgggga tttttcaaaa ggcggtagga
      841 tac
//
LOCUS       DAFCNS010000205          829 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFCNS010000205 DAFCNS010000000
VERSION     DAFCNS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.222"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..720
                     /locus_tag="LNE23_002903"
     CDS             <1..720
                     /locus_tag="LNE23_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK6932692.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
BASE COUNT          319 a          128 c          165 g          217 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatca
//
LOCUS       DAFCNS010000206          820 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFCNS010000206 DAFCNS010000000
VERSION     DAFCNS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.223"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..797
                     /locus_tag="LNE23_002904"
                     /pseudo
     CDS             1..797
                     /locus_tag="LNE23_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          287 a          153 c          149 g          231 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa gtaaatgcat
       61 ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct acttatattc
      121 ctttaaaacg gcaaaccgtc tccaaagaag caatctatat tgccattggt atacgagaag
      181 acggcactaa agaagtacta agttatgcga ttgctccgac tgaatcgacc tacgtttgga
      241 atgagttgtt acaggacatt tactccagag gggttcatga tgtcttactg tttattaccg
      301 atggcttaaa aggcatgaaa gatacgattc accagattta tcctaaagct aaataccaac
      361 attgctgtgt tcacgtttcc cgtaatattg ctcataaagt acgtgtcaaa gatcgaaaag
      421 aaatctgtga tgattttaag gctgtttatc aagctagctc aaaggaagag gcaaatacct
      481 ttttagggag tatgattgag aagtagcaaa aaacttatcc taaagtgacg cagtcactga
      541 taaaaaatca agatttattg actttttatg agtttccgcc tggtatccgc cgaagcattt
      601 actcaactaa tttaatcgaa tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag
      661 agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt gatacataca
      721 atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat acattagctt
      781 caatgtttac tgagtaaccc attattttgc agaaggacga
//
LOCUS       DAFCNS010000207          819 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFCNS010000207 DAFCNS010000000
VERSION     DAFCNS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.224"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..185
                     /locus_tag="LNE23_002905"
     CDS             <1..185
                     /locus_tag="LNE23_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932693.1"
                     /translation="NKYLKVLIESDFDRQLMNYKKDQFDLLLEKFIAIQTENTNILND
                     YVHTNNQLIETLIGGI"
     gene            187..603
                     /locus_tag="LNE23_002906"
     CDS             187..603
                     /locus_tag="LNE23_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932694.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT          326 a          163 c          113 g          217 t
ORIGIN      
        1 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
       61 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
      121 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
      181 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
      241 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
      301 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
      361 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
      421 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
      481 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
      541 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
      601 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
      661 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
      721 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
      781 ggaaatccaa agaataggac gtaccctgaa acaaaaaaa
//
LOCUS       DAFCNS010000208          804 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFCNS010000208 DAFCNS010000000
VERSION     DAFCNS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.225"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..693
                     /locus_tag="LNE23_002907"
     CDS             <1..693
                     /locus_tag="LNE23_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6932695.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          296 a          126 c          148 g          234 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct ccca
//
LOCUS       DAFCNS010000209          797 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFCNS010000209 DAFCNS010000000
VERSION     DAFCNS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.226"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            436..>797
                     /locus_tag="LNE23_002908"
     CDS             436..>797
                     /locus_tag="LNE23_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6932696.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSV"
BASE COUNT          301 a          107 c          123 g          266 t
ORIGIN      
        1 ataatatata ttttaatctt taaaacttta gcaacctcca aaaaggcttt atatcaatct
       61 taaatagatt ttagggtgga cataaagcga tgcgaacatg gacaaaaagc gatgtgaatg
      121 tggacaaaaa gcgatgcaaa cgtggacaaa aagcgatgtg gtattccgaa tatatccaaa
      181 aaaagaaaaa gtatgtgaat ttatttctaa aaactagaaa aatatttgtt ttttaatttt
      241 ggtaattttt ataatttcgt attttattga aaaggatttt tattttgaaa atgttctaaa
      301 atagcgattt ctgctgtatt ttcaatgaaa tttgattgga aaaaataaaa tgtgttttac
      361 tgacattgag ttatgttata aatttacgat acatttctta ttgtaacgta tcgaaaacag
      421 gtctatactt attcaatgga atcgtaccag ttccaagttt ttaagctcgt accaagctta
      481 gaaagaagag gcaatcgtat gaaaataaca gaaaagaatg taaacccgaa atttgaaact
      541 accgaaagca atctaaaaaa gtttgattta actgttactt tatcgaatca acttgtagat
      601 agcattgttg aaatggatgc tatgccacaa aaaatttatc tttacgctat aggacatctt
      661 gaccctaaaa atccgccttt taataatacc gtcaaagtat ctaaaaaggc tttatttgcg
      721 ttgatgtctt ctagttcatc ttcttcaaaa cattcaagat taaaacaagt tattgaaaaa
      781 ttgcatcagc aatctgt
//
LOCUS       DAFCNS010000210          793 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFCNS010000210 DAFCNS010000000
VERSION     DAFCNS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.227"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..782
                     /locus_tag="LNE23_002909"
     CDS             <1..782
                     /locus_tag="LNE23_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6932697.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          244 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc ttt
//
LOCUS       DAFCNS010000211          783 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFCNS010000211 DAFCNS010000000
VERSION     DAFCNS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.228"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(289..783)
                     /locus_tag="LNE23_002910"
                     /pseudo
     CDS             complement(289..783)
                     /locus_tag="LNE23_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          263 a          121 c          115 g          284 t
ORIGIN      
        1 ctttattaat atattttatt ggattttatt aacaattcaa attcttttgt taattagttt
       61 aaatacattc aatagcattt attagttata tgtattaaat ctaaaacaac catttcatga
      121 aaaataacat cctttctatc actattagat tcatatctct ttttttgagt taaaccagaa
      181 ataacttttt caaaataaaa ttttaacgcc aatccatgag tcaattcaaa aaaagaaaaa
      241 agattctttg ttttctcaga agttcaattc tttattattt atgatttgtc aaattaagct
      301 agaataaaat gcttcttgta catagctcaa aaatatttcg attggtgttc tgtaattcaa
      361 tgattttctt ggaatatgat tacgttgatt gctgacactg gaaataaatg tctgattcac
      421 ttctctaaaa tccattgatt tcggcagtcc attacgacgc agaatcccgt tagaattctc
      481 gtttaatggg cgttgagaag gtgttccagg gtccgcaaaa tatatatcaa tatcatgttg
      541 gttactaatg gctttccagt tagaaaattc ttttccacag tcaaacgtaa tagcgtaata
      601 gatttaaaga agtttttagg gaagcgagaa aaccattgat taagggcagt ttcaatatct
      661 aatgccttac ggccgttggg tttaatcgtg ataatgactt tagataatct ttcaactaaa
      721 gtaatgacgg cacttttatg atgaatgcca acgatcgtat caccttcaag gtgaccaaac
      781 tca
//
LOCUS       DAFCNS010000212          742 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFCNS010000212 DAFCNS010000000
VERSION     DAFCNS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.230"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            178..381
                     /locus_tag="LNE23_002911"
     CDS             178..381
                     /locus_tag="LNE23_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HBK6932698.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            381..731
                     /locus_tag="LNE23_002912"
     CDS             381..731
                     /locus_tag="LNE23_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6932699.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          300 a           79 c          120 g          243 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      481 ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg
      541 taaataaatt agttaattat atttacatcg tgggttttga taataaaata cgctttattg
      601 ataatgatga aaaattatta atagaattag gggatatttc taaaaaagct ggaattaata
      661 gtaaatacaa agcaaacttt acagataaat cgcaatttta tagttattca gaaacaatgc
      721 ctcaacgata aaatatagta ta
//
LOCUS       DAFCNS010000213          732 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFCNS010000213 DAFCNS010000000
VERSION     DAFCNS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.231"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>732
                     /locus_tag="LNE23_002913"
     CDS             <1..>732
                     /locus_tag="LNE23_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6932700.1"
                     /translation="KNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYST
                     LLRTIDDLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERD
                     IYINECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKI
                     TVNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNK
                     ISNMDKIFDVKFDLNKENSNGLGLY"
BASE COUNT          334 a           51 c          105 g          242 t
ORIGIN      
        1 taaaaaataa tatacaagaa ataaaaattg ttactgataa aatgatgaaa cttgataaaa
       61 aaatgagttt aaaagttagc tctaatgatg aagttggaga attaaaacaa caaattaatg
      121 atttgtattc tactttatta agaacaattg acgatttgga atttaaaaat aaagaaattt
      181 taaaattaga aaagttaaaa tatgactttt ttaaaggtgc atcccatgaa cttaaaaccc
      241 ctcttgctag tcttaaaata atactggaaa acatgaaata caatattgga aaatataaag
      301 aaagagatat ttatattaat gaatgtattg aaatcgttga tagtttaaca aaaaatattt
      361 ctcaaatatt atcagttcat tctatagaaa atttgaataa tgatgaagaa tatttgaaaa
      421 taaatgatac attagaagac atattaaaaa agtatgaaat attagcaaat caaaagaaaa
      481 taactgtcaa taattatata ttagacgaga atgtttatat aggaaaaaca gctttaaaga
      541 ttattctatc taatttgatt agtaatgcgg taaaatatac tgatgtaaat ggggtaatta
      601 atattgggat agttaatgat tggttatata tagagaattc atacggtaac aataaaattt
      661 cgaatatgga taaaatattt gatgttaaat ttgatttaaa taaggaaaat agtaatggat
      721 taggtttata ta
//
LOCUS       DAFCNS010000214          731 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFCNS010000214 DAFCNS010000000
VERSION     DAFCNS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.232"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LNE23_002914"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LNE23_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBK6932701.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="LNE23_002915"
     CDS             complement(481..675)
                     /locus_tag="LNE23_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932702.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DAFCNS010000215          729 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFCNS010000215 DAFCNS010000000
VERSION     DAFCNS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.233"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..413
                     /locus_tag="LNE23_002916"
     CDS             <1..413
                     /locus_tag="LNE23_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932703.1"
                     /translation="QSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYG
                     EAVTGDNGKVRVDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNV
                     LVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF"
     gene            437..>729
                     /locus_tag="LNE23_002917"
     CDS             437..>729
                     /locus_tag="LNE23_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932704.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEY"
BASE COUNT          255 a          115 c          152 g          207 t
ORIGIN      
        1 aacaatcaac ggtacaagta tataaagatt ttactgttag aggtactaaa aactcaactg
       61 taccaacaga acattatgga caacgactat tgaacgctta tgaaactcca gaatattatt
      121 tcgctgatta tggggaagcc gttacaggtg acaatggtaa agttcgtgtt gatattgacc
      181 ccatgtttgc tgaaacagta aatctaagtc ggtatatgac acatgtgaca cctacagaac
      241 tagttttgtg tgctgttact catgaagata ttgaccattt catcattgaa actagtaagc
      301 caaacgtatt agttagatgg aatttagtgg cacaccgtct agggtatgaa gatattagat
      361 taaaagagga tacagcatat gatagcacag tgcttgacca aaaacgtttt taaaacgaag
      421 acaaggaggt atataaatgg ctagcagttt atataatttg gctttagatt tcagcaaaga
      481 attaaactac accaaagcta ttatggctcg tcaaggtgat aaagggatta cggtgacggt
      541 taaaccgttt ctaaatggct tgcagatgga tacgagtggc ggaacattta ctttaaaagg
      601 aacaacacca tctaaccgtt acgtagataa tgttgcaact agtgtaacta gtgaagaagt
      661 cacgttttct cttgatggca catttatgag tgaagcagga tattataaac actgctacgt
      721 agaatatag
//
LOCUS       DAFCNS010000216          729 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFCNS010000216 DAFCNS010000000
VERSION     DAFCNS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.234"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..102
                     /locus_tag="LNE23_002918"
     CDS             <1..102
                     /locus_tag="LNE23_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6932705.1"
                     /translation="NKKNLGSLKAQQKNGAAIYREDEKEYYTKIKLM"
     gene            complement(292..636)
                     /locus_tag="LNE23_002919"
     CDS             complement(292..636)
                     /locus_tag="LNE23_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBK6932706.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          228 a          134 c          114 g          253 t
ORIGIN      
        1 aataaaaaaa atcttggcag tcttaaagca caacaaaaaa atggggctgc gatttatcgt
       61 gaagatgaga aagaatacta tacgaaaatc aaactgatgt aaaaaagaag gattccaaaa
      121 ggaaagggat ttgaatgtcc tttatcaata gaacacattt atttaattaa cttataatat
      181 agcagacaaa attttataaa caaagaaacc ttttatctct tttcttctag aaaggataaa
      241 aggctttttt gtttttccta tttgcttctc atagctagtc agccaagatt tctatcgtat
      301 ccccaacgca atccgtgcat aacggctcat cttatctgtc gtccatgctg gataccagac
      361 tagtttgact tctgctttcg ttacttctgg cacttctttt aatgcgtcat ggatagattc
      421 tgttaaaata tctgctaatg gacagcccat agttgttaga gtcatcttaa tgactgtttc
      481 tccattttca ggattgaatt ctatatcata aatcaagcct aaattgacga tgtcgattcc
      541 taattcagga tcaatcacca tttccaaggc agcaagaata cgttctttga ttagttctat
      601 ttcttgtcca gaccgttctt ggtttgcttc gctcatcgta cgacactcct taacacgatt
      661 tttatttatc atactctttt tttagctgct tgtaaatgag aatttagaag taagattctc
      721 gtttaaatt
//
LOCUS       DAFCNS010000217          712 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFCNS010000217 DAFCNS010000000
VERSION     DAFCNS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.235"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>712
                     /locus_tag="LNE23_002920"
     CDS             <1..>712
                     /locus_tag="LNE23_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HBK6932707.1"
                     /translation="SQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFI
                     KKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTK
                     TGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPP
                     RKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSV
                     LLSRAQRRFEGTLRYSLGY"
BASE COUNT          242 a          130 c          127 g          213 t
ORIGIN      
        1 tagccaaaga gccacgtata gagcggtttg ttccgtctta tttcctaatg gtgactttcc
       61 tagtaggagt cgattcactc gcttaagttc aaacttagct tacactatca agattattcg
      121 atactttttc atcaagaagc tcaccaaagg tgaactagtc ggaattatag atagttttcc
      181 tagtccatta tgtaaaccag ttagaaatag acaagcaaaa ctattgaatc aaatagccaa
      241 agttggctat aactcaacaa aaaaatctta tttttatggt cttaaaattc atatgatcgt
      301 tactaagact ggctttccga ttacttactc aatcacgaat ccaggtgtcc atgacgtgaa
      361 agtgttagag actttatcgg aagaagcgaa tcttcccaat atcctagggg acaaaggata
      421 tattagccat aaaatccatg aaaagcttgc ccttaagggc attactatat ccgttccgcc
      481 acgtaaaaac atggataaat cagaaaaact agaccacagc ttacttggaa aacaaagaaa
      541 aacagttgag actgtttttt cttccttaga aaaattaggt tgtcaaaatt tcaactcacg
      601 ttctgttaaa gggctagaga gtagatttga aagcatatta ctggcttaca gtgttctatt
      661 aagtcgagca caacgacgtt ttgaaggaac tttgagatat tctttaggat at
//
LOCUS       DAFCNS010000218          683 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFCNS010000218 DAFCNS010000000
VERSION     DAFCNS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.236"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            83..683
                     /locus_tag="LNE23_002921"
                     /pseudo
     CDS             83..683
                     /locus_tag="LNE23_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          259 a          117 c          114 g          193 t
ORIGIN      
        1 tccatgttcc ccactcctca agtgttttct ttagtatacg cctataggag gaaaaacgaa
       61 tacaatttaa ctcaatttat ataataggaa aaatttagcc gaatctgaag gaattagtag
      121 agttactcta tatcaatgga aaaacacggt tcttggaaag gattttccat tgcgtatgac
      181 tttaaaaaat caggaaaaac aaaaagagct acttttaaag gaagtagaag acttacaaaa
      241 acaagtacac caattacagc tcgaaaaagc attactcgaa ggagcggctg aactgctaaa
      301 aaaagaaaaa ggcgtcaatc ttctttgtct atcaaatcaa gaaaaaacga tattgattga
      361 tgccttacga aatcagttca ccttgaaaga gcttcttcaa cagctccagt tgcctaaaag
      421 cagctatttt tatcaaaaac aagcacttga gaaaccagat aagtattaca aagaacgtca
      481 attaattatc acgattttca atcataactt ctgtgcgtat ggttaccgta gaattgacca
      541 agctttaaaa aatatgggta aaaagctttc tgaaaaagtt gtacgtagat tgatgactga
      601 ggaaaatcta tttgttaaat tttctagacg aaaaaagtat tcttcgtatg ctggggaaat
      661 ttctcctgca caccccaatt tat
//
LOCUS       DAFCNS010000219          679 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFCNS010000219 DAFCNS010000000
VERSION     DAFCNS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.237"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          240 a          114 c           80 g          245 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatt
//
LOCUS       DAFCNS010000220          653 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFCNS010000220 DAFCNS010000000
VERSION     DAFCNS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.239"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..331
                     /locus_tag="LNE23_002922"
     CDS             <1..331
                     /locus_tag="LNE23_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBK6932708.1"
                     /translation="TKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGYTEES
                     KKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIAS
                     RFVKALS"
     gene            345..>653
                     /locus_tag="LNE23_002923"
     CDS             345..>653
                     /locus_tag="LNE23_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6932709.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     S"
BASE COUNT          233 a           79 c          146 g          195 t
ORIGIN      
        1 tactaaaata aaagaaaaat atcctcatca gttatttatg gcagatattt ctacatttga
       61 agaaggcaaa aatgcatata aagcaggaat agattttgta ggaactactt taagtggata
      121 tactgaggaa agtaaaaaac aagatgggcc agatatagat ttgattaaaa aattggtaga
      181 agaagggatt tgtgtaattg ctgagggaag gattcatact ccagaacaag caaaagagat
      241 aaaaaaaata ggagttgcag gtattgttgt gggtggtgct attactcgac cacaggaaat
      301 tgcatcaaga tttgttaaag cattaagtta gggggagaaa taaaatgttt aaaaaatttt
      361 cacaattagg acgcgctttc atgttaccaa ttgctatatt accagtagcc ggattactat
      421 tgggactggg aggagcgtta acaaatgaga gtgctataaa tgcttatcca tttttagatc
      481 aaccgtggtt gcatacaatt ttaagtatta tgagttatgc tggaaatgca gtttttgcaa
      541 acctagcact aatttttgca attggtattg ctgtaggact tgcaaatgga gataaaggaa
      601 cagctggttt agctggtgga gtagcttatc tagtatatac tgcaactatt agt
//
LOCUS       DAFCNS010000221          649 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.240, whole genome shotgun sequence.
ACCESSION   DAFCNS010000221 DAFCNS010000000
VERSION     DAFCNS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.240"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>649
                     /locus_tag="LNE23_002924"
     CDS             <1..>649
                     /locus_tag="LNE23_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6932710.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DAFCNS010000222          640 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFCNS010000222 DAFCNS010000000
VERSION     DAFCNS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.241"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..282
                     /locus_tag="LNE23_002925"
     CDS             <1..282
                     /locus_tag="LNE23_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6932711.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          219 a           89 c          119 g          213 t
ORIGIN      
        1 ttaatacaag atgatttaca ccatacgcat cggttatggt taatgaataa aattaatctg
       61 tattttgggc attcttatgt ttttgctttt tatgattaca ggacaaatat aactgaagta
      121 gatttggtta tttctaatta ctacatcgac actggaaaaa cgccattact gttaatgaaa
      181 aatataccta ccgaaagaaa ctggcgtttg ttcgagaaaa cgatttatca attgaaaaaa
      241 gaaaaagagg ttgtgaaatc agcgttttgt cctgagtatt agagaaacaa acggaaaaat
      301 agtttttcat attttgccgt ttttgagcta ataccgaggg acagctgatt gaacaccatt
      361 tattgagaga ttgtgaaaaa actgtcctgc atcaagtaat aagaacagcc aagtaaaaac
      421 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcaa gagaacaccg
      481 tttattcgga aaaagggact gtgacactac atgtcacagt ccctttttga ttgaattatc
      541 tcatttgttt tctaattttt tctttatttg agaaaatagt tgaactggat aaaaagtact
      601 ggacgtgtca aaattatttt ggatcaaatt agatctatta
//
LOCUS       DAFCNS010000223          620 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFCNS010000223 DAFCNS010000000
VERSION     DAFCNS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.242"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..496
                     /locus_tag="LNE23_002926"
     CDS             <1..496
                     /locus_tag="LNE23_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6932712.1"
                     /translation="DGETEMQTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFN
                     GLKQDGRIKPGIYSYGPDSVGEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIEN
                     GADEVAALELETKVMQDEFGKFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTA
                     TQSQ"
BASE COUNT          237 a           92 c          130 g          161 t
ORIGIN      
        1 agatggcgaa acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc
       61 aaaatctaaa ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta
      121 tcgacgtttc aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg
      181 tcctgattcc gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga
      241 aaatagaaag ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat
      301 tgaaaatggt gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga
      361 atttggtaaa ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca
      421 atggatgacg aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc
      481 cactcaatca cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg
      541 aactaaacga tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga
      601 tgatcaatag agattttaac
//
LOCUS       DAFCNS010000224          574 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFCNS010000224 DAFCNS010000000
VERSION     DAFCNS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.244"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          185 a           81 c           81 g          227 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct tttt
//
LOCUS       DAFCNS010000225          567 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.245, whole genome shotgun sequence.
ACCESSION   DAFCNS010000225 DAFCNS010000000
VERSION     DAFCNS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.245"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            79..>567
                     /locus_tag="LNE23_002927"
     CDS             79..>567
                     /locus_tag="LNE23_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBK6932713.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT          214 a          104 c           92 g          157 t
ORIGIN      
        1 aaaatccata caaaaaagga agtcccttct gtagaataaa gttaacgaca accaattcac
       61 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
      121 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
      181 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
      241 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
      301 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
      361 agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg
      421 tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg
      481 tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag agccttaaat
      541 gataagtatg tcgctatttt tatggac
//
LOCUS       DAFCNS010000226          561 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFCNS010000226 DAFCNS010000000
VERSION     DAFCNS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.246"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>561)
                     /locus_tag="LNE23_002928"
     CDS             complement(<1..>561)
                     /locus_tag="LNE23_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBK6932714.1"
                     /translation="FLLVKWIAPFLLSFAEKVYANSSIIIVSLVICLGMSYVADLVGL
                     SSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFVSVGLEVDFSDFSEQLLFI
                     SILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRGEMALIILQIGQQSQLIEP
                     QYYSPLVIVILLSTLISPLVLKYFTKK"
BASE COUNT          215 a          111 c           89 g          146 t
ORIGIN      
        1 tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata aaatcacgat
       61 aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa tttgtaaaat
      121 gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg aactatgttg
      181 agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca aaatcgctac
      241 aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat caacttctaa
      301 tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct ctacattgtg
      361 gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa aagcaccaat
      421 gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa taactagcga
      481 tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta ggaaaggcgc
      541 aatccatttg actaataaga a
//
LOCUS       DAFCNS010000227          548 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFCNS010000227 DAFCNS010000000
VERSION     DAFCNS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.247"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>548)
                     /locus_tag="LNE23_002929"
     CDS             complement(<1..>548)
                     /locus_tag="LNE23_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK6932715.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DAFCNS010000228          540 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFCNS010000228 DAFCNS010000000
VERSION     DAFCNS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.248"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..540
                     /locus_tag="LNE23_002930"
                     /pseudo
     CDS             1..540
                     /locus_tag="LNE23_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          152 a          136 c          133 g          119 t
ORIGIN      
        1 gtgccaattg gacattggcg attgtgtgtg acctgattct ccgggaatgg agttatggat
       61 atacgcaaaa aggcgtggcg gacctccttc accggctagg actaagctgg acccgtccga
      121 cgtataccct caaaaaagcg gattccaaga aacaacaggt atttttggaa gaaaccttcc
      181 cgagcctaaa aaaaaattgc tgaatagcga tattgaccac ctcctattcg aggacgaaac
      241 catgatccgg gattaccaag ccattggctg cacctggttt ctccgcggcc accagcgaaa
      301 aatccccact tatggacaac acaaaggggt aaaattgata ggcaccttgg attatgaaac
      361 cggtcggatc atttgtacag aagaaggggc atacgacgca gcggccttcc tccgttttct
      421 ggagcggacg ttgcgggaat accccaccgg aaaaatcgtg atggtattgg ataatgccct
      481 tatccaccac gccaagctga tccagtcttt tcttgaagaa aacgctaggc gcctggcact
//
LOCUS       DAFCNS010000229          528 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.249, whole genome shotgun sequence.
ACCESSION   DAFCNS010000229 DAFCNS010000000
VERSION     DAFCNS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.249"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..90)
                     /locus_tag="LNE23_002931"
     tRNA            complement(5..90)
                     /locus_tag="LNE23_002931"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="LNE23_002932"
     tRNA            complement(107..178)
                     /locus_tag="LNE23_002932"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="LNE23_002933"
     tRNA            complement(190..262)
                     /locus_tag="LNE23_002933"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="LNE23_002934"
     tRNA            complement(280..361)
                     /locus_tag="LNE23_002934"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="LNE23_002935"
     tRNA            complement(369..441)
                     /locus_tag="LNE23_002935"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="LNE23_002936"
     tRNA            complement(445..517)
                     /locus_tag="LNE23_002936"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAFCNS010000230          514 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.251, whole genome shotgun sequence.
ACCESSION   DAFCNS010000230 DAFCNS010000000
VERSION     DAFCNS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.251"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(107..>514)
                     /locus_tag="LNE23_002937"
     CDS             complement(107..>514)
                     /locus_tag="LNE23_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBK6932716.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DAFCNS010000231          512 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.252, whole genome shotgun sequence.
ACCESSION   DAFCNS010000231 DAFCNS010000000
VERSION     DAFCNS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.252"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..343
                     /locus_tag="LNE23_002938"
     CDS             <1..343
                     /locus_tag="LNE23_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6932717.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           69 c           93 g          144 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggccctata at
//
LOCUS       DAFCNS010000232          505 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.253, whole genome shotgun sequence.
ACCESSION   DAFCNS010000232 DAFCNS010000000
VERSION     DAFCNS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.253"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          226 a           70 c           81 g          128 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actct
//
LOCUS       DAFCNS010000233          500 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.254, whole genome shotgun sequence.
ACCESSION   DAFCNS010000233 DAFCNS010000000
VERSION     DAFCNS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.254"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>500
                     /locus_tag="LNE23_002939"
     CDS             <1..>500
                     /locus_tag="LNE23_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HBK6932718.1"
                     /translation="SLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFK
                     IEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEI
                     VILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDS
                     DPLLQY"
BASE COUNT          179 a           82 c          100 g          139 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta
//
LOCUS       DAFCNS010000234          480 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.256, whole genome shotgun sequence.
ACCESSION   DAFCNS010000234 DAFCNS010000000
VERSION     DAFCNS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.256"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>480
                     /locus_tag="LNE23_002940"
     CDS             <1..>480
                     /locus_tag="LNE23_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6932719.1"
                     /translation="HMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANIN
                     HYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFS
                     LLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGF"
BASE COUNT          169 a           98 c           92 g          121 t
ORIGIN      
        1 acacatgaat caagccttta atgagttgcg tatccgtgaa atgaatgaac ttcgtaaagt
       61 aggacagaaa agtcaggcag aaaaactgaa aaagaactgg cgctttttgc taaaaaatcg
      121 tgcaaacatc aaccattatg aatacaaaac atggaaaagt tttcgagcac caaaataccc
      181 atttcttacg gaagcgatga tgattgatcg attgcttggc ttttctacgt ccctgaagga
      241 ggcgtatccc tattttcatg aattagttga agcctttcga gacaaagacc ctgacttatt
      301 tttctcctta ttggcagaac ttcccgaaac gttagatgac ggctttcggg aaaagctcca
      361 aaaccttctg acctatgaag aaggcatcac caacgcgatg atctatcctt acaccaatgg
      421 aaaaatagaa gcgaagaata cccatataaa gacaatgaaa cgagtatcct acggatttaa
//
LOCUS       DAFCNS010000235          470 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.257, whole genome shotgun sequence.
ACCESSION   DAFCNS010000235 DAFCNS010000000
VERSION     DAFCNS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.257"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>470
                     /locus_tag="LNE23_002941"
     CDS             <1..>470
                     /locus_tag="LNE23_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HBK6932720.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT          167 a           78 c           88 g          137 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc
      361 atactgatcg tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg
      421 acatcaatcg atcattgagt cataaaggct gtccttttga taatgccgta
//
LOCUS       DAFCNS010000236          462 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.258, whole genome shotgun sequence.
ACCESSION   DAFCNS010000236 DAFCNS010000000
VERSION     DAFCNS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.258"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..430)
                     /locus_tag="LNE23_002942"
     CDS             complement(<1..430)
                     /locus_tag="LNE23_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6932721.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAFCNS010000237          460 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.259, whole genome shotgun sequence.
ACCESSION   DAFCNS010000237 DAFCNS010000000
VERSION     DAFCNS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.259"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(20..91)
                     /locus_tag="LNE23_002943"
     tRNA            complement(20..91)
                     /locus_tag="LNE23_002943"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="LNE23_002944"
     tRNA            complement(113..200)
                     /locus_tag="LNE23_002944"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="LNE23_002945"
     tRNA            complement(225..298)
                     /locus_tag="LNE23_002945"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="LNE23_002946"
     tRNA            complement(312..382)
                     /locus_tag="LNE23_002946"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="LNE23_002947"
     tRNA            complement(384..459)
                     /locus_tag="LNE23_002947"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAFCNS010000238          450 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.260, whole genome shotgun sequence.
ACCESSION   DAFCNS010000238 DAFCNS010000000
VERSION     DAFCNS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.260"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>450
                     /locus_tag="LNE23_002948"
     CDS             <1..>450
                     /locus_tag="LNE23_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6932722.1"
                     /translation="SGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSV"
BASE COUNT          152 a           75 c           92 g          131 t
ORIGIN      
        1 agcggaattt ggaacggtat caaaaatact gctttgaatc tttggaatgg aattacaagc
       61 actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg gtccaatcta
      121 acaggtattg tttccgacat attcaataat gttaaaagta caattgctaa catatgggaa
      181 ggcatcaaaa agactgttag cgctccaatt gattggatta gagacaaaat cagtggcatt
      241 tttgataatt tgaatatttc tataccacat attccgttac cggaatttat catggaaggc
      301 agctttaacc cgctaaaagg tcaaatcccc catttgcgtg taaaatggca tgctaaagga
      361 ggtatcttta ctaaaccaac tttactaggt ggaatgaacg gtgtcggtga agcaggacct
      421 gaagcagttt tacctttgaa aagatctgtt
//
LOCUS       DAFCNS010000239          438 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.261, whole genome shotgun sequence.
ACCESSION   DAFCNS010000239 DAFCNS010000000
VERSION     DAFCNS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.261"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>438
                     /locus_tag="LNE23_002949"
     CDS             <1..>438
                     /locus_tag="LNE23_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6932723.1"
                     /translation="SGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSV"
BASE COUNT          145 a           67 c           93 g          133 t
ORIGIN      
        1 agcggaattt ggaacggtat caaaaacact gctttgaatc tttggaatgg aattacaagc
       61 actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg gtccaatcta
      121 acaggtattg tttccgacat attcaataat gttaaaagta caattgctaa catttgggaa
      181 ggtatcaaaa agactgttag cgctccaatt gattggatca gagataaaat cagtagtatc
      241 tttgataatt tgaatatttc tataccacat attccgttac cacattttaa attgagcgga
      301 gaattcaatc cattgaaggg gaaaatccca acgttgggtg ttgattggta tgcgaaaggt
      361 agtgtgttta attctccgaa tattatcggt gtcggcgaag caggacctga agcagtttta
      421 cctttgaaaa gatctgtg
//
LOCUS       DAFCNS010000240          415 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.262, whole genome shotgun sequence.
ACCESSION   DAFCNS010000240 DAFCNS010000000
VERSION     DAFCNS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.262"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..339)
                     /locus_tag="LNE23_002950"
     CDS             complement(<1..339)
                     /locus_tag="LNE23_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6932724.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAFCNS010000241          406 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.263, whole genome shotgun sequence.
ACCESSION   DAFCNS010000241 DAFCNS010000000
VERSION     DAFCNS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.263"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>406
                     /locus_tag="LNE23_002951"
     CDS             <1..>406
                     /locus_tag="LNE23_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6932725.1"
                     /translation="FICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMD
                     MNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRL
                     KRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTE"
BASE COUNT          128 a           96 c           71 g          111 t
ORIGIN      
        1 tttatttgtg ttgatgggac aaccaatcag ctatttacga tccttgaaga tcgcagactt
       61 tacaaactaa ctcaatactt cctgcgcttt cctagaaagg cccgtttaaa ggtcaagtac
      121 ctagtgatgg acatgaatgc cagctattgt cagttgctta aaacagtctt tccaaatgcg
      181 gagatcgtaa ctgaccgctt tcatattgtc caacacatca atcgcagctt caatcaatta
      241 cgtgtccaga tcatgaatcg ttttcgaacc tcacactctg aaaatcagaa gaagtaccgt
      301 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccactcaaa
      361 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaa
//
LOCUS       DAFCNS010000242          405 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.264, whole genome shotgun sequence.
ACCESSION   DAFCNS010000242 DAFCNS010000000
VERSION     DAFCNS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.264"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..382
                     /locus_tag="LNE23_002952"
     CDS             <1..382
                     /locus_tag="LNE23_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6932726.1"
                     /translation="KEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIEN
                     QDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYN
                     QKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          157 a           72 c           63 g          113 t
ORIGIN      
        1 aaaagaaatc tgtgatgact ttaaggctgt ttatcaagct aactcaaaag aagaagcgaa
       61 taccttctta tccggcatga ttgagaaatg gaagaaaaac tatcctaaag tgacgcagtc
      121 actcatagaa aaccaagact tattaacttt ttatgatttt ccacctagca ttcgtagaac
      181 catttactca accaatctaa tcgagtcttt caataagcaa attaaaagat acagccgtag
      241 aaaagagcag tttcaaaatg aagaatcact agaacgcttt ctagtcagca tttttgatac
      301 atacaatcaa aaatttctaa acagaagcca taaaggtttt caacaggtaa ccgatacatt
      361 agtttcaatg tttactgagt aactaattat tttgcaggag gacaa
//
LOCUS       DAFCNS010000243          381 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.265, whole genome shotgun sequence.
ACCESSION   DAFCNS010000243 DAFCNS010000000
VERSION     DAFCNS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.265"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..94)
                     /locus_tag="LNE23_002953"
     tRNA            complement(21..94)
                     /locus_tag="LNE23_002953"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="LNE23_002954"
     tRNA            complement(108..181)
                     /locus_tag="LNE23_002954"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="LNE23_002955"
     tRNA            complement(209..281)
                     /locus_tag="LNE23_002955"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="LNE23_002956"
     tRNA            complement(301..374)
                     /locus_tag="LNE23_002956"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          104 a          108 c           89 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 aatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DAFCNS010000244          368 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.266, whole genome shotgun sequence.
ACCESSION   DAFCNS010000244 DAFCNS010000000
VERSION     DAFCNS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.266"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>368
                     /locus_tag="LNE23_002957"
     CDS             82..>368
                     /locus_tag="LNE23_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBK6932727.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          130 a           59 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcg
//
LOCUS       DAFCNS010000245          343 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.268, whole genome shotgun sequence.
ACCESSION   DAFCNS010000245 DAFCNS010000000
VERSION     DAFCNS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.268"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            46..>343
                     /locus_tag="LNE23_002958"
     CDS             46..>343
                     /locus_tag="LNE23_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6932728.1"
                     /translation="MQTFLTKTSFKKVVLLDYLLEKDNWCAMEELRNLLNVTEKSVLH
                     YIEELEDLFKQYNGNILLKNEDNKRFFIKKEKDFPIYNIYLHFYKASYNYHLIDF"
BASE COUNT          138 a           32 c           46 g          127 t
ORIGIN      
        1 attctttgat taaaataaac aaaagtaact tattgaggtg aaatgatgca aactttttta
       61 actaaaacat ctttcaaaaa agtagtttta ttagattatt tgttagaaaa agacaattgg
      121 tgtgcaatgg aagaacttag aaatttactt aatgttacgg agaagtcagt cttacactat
      181 atagaagaac tagaggattt atttaagcaa tataatggta atatccttct gaaaaatgaa
      241 gataataaac gatttttcat aaaaaaagag aaagattttc ctatttataa tatttatctt
      301 catttttata aagcttctta taattatcat ttgattgatt tta
//
LOCUS       DAFCNS010000246          333 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.269, whole genome shotgun sequence.
ACCESSION   DAFCNS010000246 DAFCNS010000000
VERSION     DAFCNS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.269"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          124 a           54 c           59 g           96 t
ORIGIN      
        1 ttaagattaa tgacactaaa ttaacagact atacgtagta tctatttgct atgaccatat
       61 cttatatcca taagtcaact cagccggact aaaagggcta atcttactag aacacaagct
      121 gcacttgata tgcaaattaa ccctgaatcg gaaaggtgct taaattggat attaacggag
      181 ggataaagta aaatttatct ctagggaatg tttagaaagg atccaagttc cttaacaagt
      241 gacaattagt aatgtagtaa caacgatagg ttgtatgccg aacatttcaa tttgatacgt
      301 ataaaagacg atttcaagct catccgtgat aaa
//
LOCUS       DAFCNS010000247          332 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.270, whole genome shotgun sequence.
ACCESSION   DAFCNS010000247 DAFCNS010000000
VERSION     DAFCNS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.270"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>332
                     /locus_tag="LNE23_002959"
     CDS             <1..>332
                     /locus_tag="LNE23_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932729.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DAFCNS010000248          328 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.271, whole genome shotgun sequence.
ACCESSION   DAFCNS010000248 DAFCNS010000000
VERSION     DAFCNS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.271"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>328)
                     /locus_tag="LNE23_002960"
     CDS             complement(<1..>328)
                     /locus_tag="LNE23_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6932730.1"
                     /translation="RAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYV
                     IHAFLDGLSFYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFI
                     YFFVFTF"
BASE COUNT          141 a           60 c           44 g           83 t
ORIGIN      
        1 agaatgtaaa aacaaagaaa tatataaatg cccatgcaat accaaaagga attaatttta
       61 accaattagt tttgtcattc ccttgaagta caccgaataa taaaaagtcg atcaaaccac
      121 ctgaaaagga attccctaca cgaatattta agatatcagc aaaataaaaa gataatccat
      181 ctaagaatgc atgaatcaca taaagccaag gagcaacaaa taaaaacata tattcaactg
      241 gttcagtaat tccagttaaa aatgatgtaa gggccccgct aaaataaagc ccaccattct
      301 ttttacgatt tttcttagga attgcacg
//
LOCUS       DAFCNS010000249          323 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.272, whole genome shotgun sequence.
ACCESSION   DAFCNS010000249 DAFCNS010000000
VERSION     DAFCNS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.272"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="LNE23_002961"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="LNE23_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBK6932731.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAFCNS010000250          321 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.273, whole genome shotgun sequence.
ACCESSION   DAFCNS010000250 DAFCNS010000000
VERSION     DAFCNS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.273"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(69..321)
                     /locus_tag="LNE23_002962"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="LNE23_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAFCNS010000251          309 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.274, whole genome shotgun sequence.
ACCESSION   DAFCNS010000251 DAFCNS010000000
VERSION     DAFCNS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.274"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>309
                     /locus_tag="LNE23_002963"
     CDS             <1..>309
                     /locus_tag="LNE23_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932732.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     E"
BASE COUNT           96 a           55 c           60 g           98 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagag
//
LOCUS       DAFCNS010000252          295 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.277, whole genome shotgun sequence.
ACCESSION   DAFCNS010000252 DAFCNS010000000
VERSION     DAFCNS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.277"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            76..>295
                     /locus_tag="LNE23_002964"
     CDS             76..>295
                     /locus_tag="LNE23_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6932733.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT          111 a           57 c           51 g           76 t
ORIGIN      
        1 agacaaataa aaaagccatt tatgttacac tcaaattgta atacccgcta aagcatacaa
       61 ggagagtgaa cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa
      121 gcatattttc atcaagaaac tccggttgct atcgttgcga agcagcttaa acgtggacgc
      181 caaacaattt acaatgtcta taactttctc aaatgtggtg gaacagcact tgaatacttt
      241 gaacaataca aagaaaataa gcgacgttgt gggagaaccg aaatcatttt tcctg
//
LOCUS       DAFCNS010000253          292 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.278, whole genome shotgun sequence.
ACCESSION   DAFCNS010000253 DAFCNS010000000
VERSION     DAFCNS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.278"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..185
                     /locus_tag="LNE23_002965"
     CDS             <1..185
                     /locus_tag="LNE23_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6932734.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DAFCNS010000254          290 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.279, whole genome shotgun sequence.
ACCESSION   DAFCNS010000254 DAFCNS010000000
VERSION     DAFCNS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.279"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            37..>290
                     /locus_tag="LNE23_002966"
     CDS             37..>290
                     /locus_tag="LNE23_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6932735.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           88 a           53 c           57 g           92 t
ORIGIN      
        1 atagatttat gaaaacgaat gcaaggagtg atagctatga tgtctaaaaa caatacgaat
       61 ggtagaaatc aatttgcgat gcttacgatt gatgatctag ttcctcaaga tcatctcgtt
      121 cgaaagattg atgccgcgct tgattttgaa tttatttatc caatcgtcga agccacttac
      181 tccgatttag gtcgcccaag tatcgatcca gtgattctga tcaaattggt gtttattcag
      241 tatttattcg gtatccgttc catgcgacaa accattaagg aagtcgatac
//
LOCUS       DAFCNS010000255          265 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.281, whole genome shotgun sequence.
ACCESSION   DAFCNS010000255 DAFCNS010000000
VERSION     DAFCNS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.281"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>265
                     /locus_tag="LNE23_002967"
     CDS             <1..>265
                     /locus_tag="LNE23_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBK6932736.1"
                     /translation="KAGFVTEDNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELED
                     EINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          106 a           44 c           61 g           54 t
ORIGIN      
        1 aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat caaagctaac
       61 gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc ctatcaggat
      121 gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc ttttactttg
      181 gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga tccggaaagc
      241 ggctactacg tcaaaggcga acgag
//
LOCUS       DAFCNS010000256          254 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.283, whole genome shotgun sequence.
ACCESSION   DAFCNS010000256 DAFCNS010000000
VERSION     DAFCNS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.283"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            9..>254
                     /locus_tag="LNE23_002968"
     CDS             9..>254
                     /locus_tag="LNE23_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK6932737.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRN"
BASE COUNT           76 a           60 c           53 g           65 t
ORIGIN      
        1 acttccatat gaacgatttt actacagaaa ttctaaagac tctagcgaac aaaggcgatt
       61 tgaatgaatt attccgtgtc catttggaaa aagctgtcaa tacgcttctc aaaacggagt
      121 taacggcttt cctcgattac gaaaagtacg atcgcattgg ttttaacacg gggaattctc
      181 gtaacggctc ctatgaccgt acggtcaaga ccgagtacgg ggaacttcat ctccagattc
      241 cgcgcgaccg caac
//
LOCUS       DAFCNS010000257          247 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.denovo.285, whole genome shotgun sequence.
ACCESSION   DAFCNS010000257 DAFCNS010000000
VERSION     DAFCNS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.denovo.285"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>247
                     /locus_tag="LNE23_002969"
     CDS             <1..>247
                     /locus_tag="LNE23_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6932738.1"
                     /translation="SKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEF
                     SNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           88 a           47 c           43 g           69 t
ORIGIN      
        1 tttcaaaagc cattatcgcc ttgaaaccag aaggccgtaa ggcagttgat attgaaaatt
       61 cgattaatga atggcttcaa tccgtaccca aaaatctttt caaatcaatt acctttgatt
      121 gtggaaaaga attctctaat tggaaaagta ttagtaatac gaatgatatt gatatttatt
      181 tcgcagaccc aggaacgcct tcccaacggg gattaaatga acattcaaac ggactcctcc
      241 gaaaaga
//
LOCUS       DAFCNS010000258        19825 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.054, whole genome shotgun sequence.
ACCESSION   DAFCNS010000258 DAFCNS010000000
VERSION     DAFCNS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.054"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(134..577)
                     /locus_tag="LNE23_002970"
     CDS             complement(134..577)
                     /locus_tag="LNE23_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBK6932739.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="LNE23_002971"
     CDS             complement(795..2135)
                     /locus_tag="LNE23_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6932740.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="LNE23_002972"
     CDS             2404..3300
                     /locus_tag="LNE23_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBK6932741.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="LNE23_002973"
     CDS             complement(3450..4391)
                     /locus_tag="LNE23_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK6932742.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="LNE23_002974"
     CDS             4517..5638
                     /locus_tag="LNE23_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK6932743.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="LNE23_002975"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="LNE23_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK6932744.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="LNE23_002976"
     CDS             7426..7992
                     /locus_tag="LNE23_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6932745.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="LNE23_002977"
     CDS             8203..9264
                     /locus_tag="LNE23_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK6932746.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="LNE23_002978"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="LNE23_002978"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK6932747.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="LNE23_002979"
     CDS             10177..12159
                     /locus_tag="LNE23_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6932748.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="LNE23_002980"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="LNE23_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK6932749.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="LNE23_002981"
     CDS             12881..14305
                     /locus_tag="LNE23_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6932750.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="LNE23_002982"
     CDS             complement(14486..15259)
                     /locus_tag="LNE23_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK6932751.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="LNE23_002983"
     CDS             complement(15256..16161)
                     /locus_tag="LNE23_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6932752.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="LNE23_002984"
     CDS             complement(16315..16635)
                     /locus_tag="LNE23_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBK6932753.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="LNE23_002985"
     CDS             17109..17795
                     /locus_tag="LNE23_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBK6932754.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="LNE23_002986"
     CDS             17797..18741
                     /locus_tag="LNE23_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBK6932755.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="LNE23_002987"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="LNE23_002987"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBK6932756.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..>19825
                     /locus_tag="LNE23_002988"
     CDS             19663..>19825
                     /locus_tag="LNE23_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932757.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRY"
BASE COUNT         6349 a         3586 c         4129 g         5761 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatc
//
LOCUS       DAFCNS010000259        14215 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.066, whole genome shotgun sequence.
ACCESSION   DAFCNS010000259 DAFCNS010000000
VERSION     DAFCNS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.066"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            105..956
                     /locus_tag="LNE23_002989"
     CDS             105..956
                     /locus_tag="LNE23_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6932758.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(1207..3165)
                     /locus_tag="LNE23_002990"
     CDS             complement(1207..3165)
                     /locus_tag="LNE23_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6932759.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3162..4034)
                     /locus_tag="LNE23_002991"
     CDS             complement(3162..4034)
                     /locus_tag="LNE23_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6932760.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(4376..5248)
                     /locus_tag="LNE23_002992"
     CDS             complement(4376..5248)
                     /locus_tag="LNE23_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6932761.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            5376..5945
                     /locus_tag="LNE23_002993"
     CDS             5376..5945
                     /locus_tag="LNE23_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBK6932762.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            6012..6749
                     /locus_tag="LNE23_002994"
     CDS             6012..6749
                     /locus_tag="LNE23_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6932763.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            6980..8248
                     /locus_tag="LNE23_002995"
     CDS             6980..8248
                     /locus_tag="LNE23_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK6932764.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            8735..9199
                     /locus_tag="LNE23_002996"
     CDS             8735..9199
                     /locus_tag="LNE23_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6932765.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            9183..9812
                     /locus_tag="LNE23_002997"
     CDS             9183..9812
                     /locus_tag="LNE23_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK6932766.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            10166..11644
                     /gene="msr(C)"
                     /locus_tag="LNE23_002998"
     CDS             10166..11644
                     /gene="msr(C)"
                     /locus_tag="LNE23_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK6932767.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            11691..12137
                     /locus_tag="LNE23_002999"
     CDS             11691..12137
                     /locus_tag="LNE23_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK6932768.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(12336..12851)
                     /locus_tag="LNE23_003000"
     CDS             complement(12336..12851)
                     /locus_tag="LNE23_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6932769.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            12968..14169
                     /locus_tag="LNE23_003001"
                     /pseudo
     CDS             12968..14169
                     /locus_tag="LNE23_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         4464 a         2569 c         2929 g         4253 t
ORIGIN      
        1 tttttttgct tgacttatct taccacactc tttatgataa aggtgtaatt tataaatgta
       61 cagctgtaaa tcttaacaaa taaaaagaat agggagtggc aaatatgcat tatttagtaa
      121 caggagcaac tggaggtttt ggtggttatg cgctggaatt cttaaaaaag atggttccac
      181 aaaatgaaat ctatgtacta gtacgtagtg aagaaaaagg agaagcactg aaagaagctg
      241 gatttaatat acgtattggt gattatgatg atttagactc aatgaaagaa gcgttgaaag
      301 gaatagatcg attgcttttt gtctctggcg ttccaggaaa tcgtcaagcc gaacataaga
      361 atgtagtgaa ggcagcacaa gcagctgggg tatcttatat cgcttataca agttttgcag
      421 atgccgatca ctcgatcagt gtattagcgc cagatcacca attcactgaa aaaatgatta
      481 aggattcagg tatttcccat acatttttac ggaataattg gtatgtagag aatgaattac
      541 ctatcattgg gcaggcttta aaaactggtc aattcgttta tgctgcagga aatggaaaaa
      601 caggttgggc attgaaacgt gaatatgcag aagctgcagc aaaagtacta gtagaaagtg
      661 actctccaga aatcttagaa ttatccggca atctaactga ctatgaagaa ttagccaagg
      721 cattagagcg tgcgacaggt aaagagttag aaattattga agcaagtgat gcagcgtttg
      781 tcgaaaatct aaaagaagcc ggattccctc aagaagcagc tgacatgttt ttagcgatcc
      841 aacatgatat taaaaaggat cagttagcta tttcctctga tgatttagaa aaagtattgg
      901 gaaaaccatt aacttctctt gaagcagcat taaaagaaat attggatcag gaataatgta
      961 taaagaggtt gtgaaaaagc cgattagttt tgagtatgaa gaaaaatttt gtgaaaaagc
     1021 tgttctgcat caagtaataa gaagagtcaa ataaaaaata gctcttcata tgtttcgttt
     1081 agctggactt attaccgcgg agttggcagg agaacgccgt ttttattcgg aaaaaggagc
     1141 tgagacaaga tttttgtctc agctcctgtt tgtttatcga taaaaaagtc tggggaagac
     1201 gatggctcag gctttgttgg aaacagagcc gattttatca tttgctttta catcacgtgc
     1261 gggttcgatt tctaagcttt ggactttgtc gccatttgtg aatacaacga ccatatccgt
     1321 gtttttgcct gcagattgaa tagcggcaag atcaactaaa gcaatcaatt gacctcttgc
     1381 gactttttga ccttcttcta catataaagt gaatggttcg ccttttaagt caacagtgtt
     1441 gatgcccata tgaagcaaga cttcgatacc agcatctgtt tggattccga cagcatgttt
     1501 cgttgggaaa atgtttgtga tcgtaccagc gattggagag aagatttctc cattttctgg
     1561 taatacagca tagccgtcac ccatcatttt ttccgcgaat acatcatctg aaacttcagt
     1621 gattggaatc agtttcccat ttgcgatagc gtaaatatct tgttcgatcg tttcaccggc
     1681 aaatgtttct gttttaacca cggtgtttgt ttgtgcaggt cgtgctgcag caccaccatg
     1741 tagttttgcc atctcatctg caacgaattg tacttctgta cctacgatga cttgcgcatt
     1801 ttttccgtcg atgatcttca taccaggtac gccagtggct ttgattttat tttgatcgat
     1861 caggcctgta tctttgactt gcaaacgaag gcgtgtagta cagttatcga tcgaagttac
     1921 attgtcggct ccgcctaatg cagcgtagat tttgcttgct tgtgccgcat atttattgtc
     1981 tgaattagtc gttgcagtat cgtcatctga aagatctgct tcctcgcggc caggagtcat
     2041 cagatggaat tttttgatag caaagttaaa gccaaagtag taaatcactg ctgttactaa
     2101 tccttggacg atcaacatca atggctgatt cgcaatcgga tttttcaaag ataggaagta
     2161 atcgacaaat ccggcactaa aggcaaatcc tgctgtccaa tggaagaatg cactcactgc
     2221 taaagaaatc ccagtgaaaa ttgcatgcaa gacatataat ggccaagcaa cgaacataaa
     2281 tgaaaattca agtggttctg tcacaccagt aaagaatgaa gcaaaagctg ctgctagcat
     2341 caaggaagct gtttttgctt tgttttctgg acgtgcattt ctgtaaatag catatgctcc
     2401 tgctggaagc ccgaacatca tgattgggaa aaatccagct tgatacattc ctgttacgcc
     2461 ttttgttcct tcagaagcca aaaatttacc aatatcattg attcctgcta cgtcaaacca
     2521 gaacacggag ttcattgctt gatgcaatcc tgtagggatc agtaaacggt tgaagaagcc
     2581 ataaagacct gcaccacccc agcctaaact caaaatgaat ttagcaaaag aaactaaagc
     2641 agagtaaacc ggaggccata cgagcagtag cactgcactg atcagcaaca tgacaaaggc
     2701 agccaagatt ggcactagac gcttgccgct aaagaaagat aaagccatcg gtaattttgc
     2761 ttcgtggaaa cgattataaa gagctgccgc aatcaatccg gcaataatac caataaatac
     2821 gttactgatc tgagcaaaag atggatcaac tgattctggt ttgatattca ataaagtagc
     2881 tactgaatca gtttttaaaa catttactgg aacttcaaat gcgaccaaac ctgccaaggc
     2941 agcagcacct gatttgtcca ctgacatccc tagtgctaac ccaacggcaa acagcaaagg
     3001 taattgacct aaaactgcgt taccgccagc ttgtaaaaaa tgagcgacaa tgtcgttact
     3061 ataagatgcc caccagtttc cgatccctac tagtaaagca gcagcaggta agaccgcaac
     3121 tggaagttga agggaacgtc ccattcgttg tagatatgct ttcaattttt ttcctcctca
     3181 tgattgttaa catttgtttg tacgattgcg cttcttaaac gctcgatatg gatggccaag
     3241 taacccagtt cttctttcgt gatcgcgagc tggtattctt tggtcagcaa cacacggatt
     3301 tttttagaaa tcttatagct ttctgggtat tttcgtttga ccatttctac gatttccgtg
     3361 tctaaaacag catattgttg cgcatgggtt cgttgtagaa gcagtcgtaa atgattcacc
     3421 agtcttgaat aattcagtga aaatggttct gaatggatat ctgtttccaa ttctgattcc
     3481 actaaatgga tgatgtcaga tacaatcgtc acttcacgga tactgcgatg gttattgttc
     3541 cgaccgcttc gtccgctgtg aatatggatt gcgatatatc ctgcttcgtc ataagtatat
     3601 ggaatcccaa gctcttttgt cagatactct actgcccatt gagcaatact gaattcatca
     3661 ctgtaaagca cttcgatttc tcttaaaagt ttgttgcgga tgacgatacc gttattaatg
     3721 ttctccgcag aaaaagcaat gtggtcggtc aaagcgatca atacatgttc attcaacttt
     3781 tccatcaaaa cagtttccgc atgatcaatg atcgtttctg ctgcaaataa aaagcgttca
     3841 tcaatctgat taagtaaatt ttgcaacttg atttgacctt ctggttccat gacaaatatc
     3901 cgttcgacat cacgatcaaa aatcaaatca tttctttttc tgtcaaatcc aatccctttt
     3961 ccaatagcta ctttttcctg tccaccgtca tctaccaata ctgcattttg attaaagatc
     4021 tttttgattt tcatgcgcgc accctttttg aagatttggt tttcacgttt gctagaccag
     4081 ttgattgcgg ccaaacaaaa atagacacga aaaacctcct agggaaacca tgttcctcag
     4141 agatttttca tgcctagtat gatctagtca catgaaacga attattgtta acgtaatcaa
     4201 ttttatatag acagttatgt tttgtcaatt ctttttttat aattatagta atttactgga
     4261 attatcagaa ataaaaggaa gaaataaatt aagaaactta tgttatttaa tcgttggatc
     4321 ttttgcttat cttattcgtt gttaaaaagc tgaaagcgaa ggaaaaaaac tctacttaac
     4381 tgtatttctc cgttaggaca tctaaaaatt tacgagcagc aggagaaaga ggctggtctt
     4441 ttttccaaat gagattcagt gaagcttgta atggcggatc gagtgggatg aatatttttt
     4501 ccgtcccata agtattgatg atcccatcta gagctaatgc accaaaagtc ccgttatcga
     4561 ccatcaaaga agcattgtac aataagttat atgttccgac gatcgataac tgatctatgc
     4621 tgaaacctag ccattcagct agttgactgt ccactgacgt ttgccgggaa atgatcaaag
     4681 gaacagtttt cagttcctgc ggatggatga cggtttgctt tgctaaagga tgatttcgat
     4741 gtatcaaaag tccccaacga tcttgggctg gcagtcgaaa tgactggtat ttttgtttgt
     4801 ttgtcggttc gattacgagt ccaaaatcaa gtaatccgct atccagtttg tctatgatat
     4861 catctgcatt cccgctatat aaatgaaact ggatatcagg atgcgccttg atcaacgaat
     4921 gaaggctatc agaaatgaaa gagattgctt tagtttcgcc tccgccaatg tagatttctc
     4981 cagagatgat tgcgtcattt gttaaattgt tcgccgtttt ttctaccaac gacaagattt
     5041 cttttccttt tgaataaaga tatcgaccag aatccgtcaa ctgtatctct cgggagcccc
     5101 gagtgaaaag tgtcacacca agttcttctt ctaaatcttt cagttgtttt gagagagtag
     5161 gttgcgtgat atgcagagat tttgctgctc ggctgatatt tttttctcta gctactgcta
     5221 aaaaatacgt cagtatacga atatccattt tttccctcct gtttttaaaa aagcatacca
     5281 ctaagtatag ttaaaaggaa tagttaagta tgttttataa gtattagaca tgataagccg
     5341 tccgttataa agtaaggata aaaggaggga gcaaaatgac aagcctcatc gaaaaacaaa
     5401 tcgtcaataa agagattcga gtaacggatc cgctctttga agtgatccat cagatccaag
     5461 ctgaaaacga agagccttta gcagcgctga atactgggta ccatgaacca gcagatatcc
     5521 gcaaacggct agaaaatatc attagtgaca aagtagatga tacggtgact gttctgctgc
     5581 ctttttatac agatttcggc aaacatatta gtatagggaa aaatgtcttt atcaatcgtc
     5641 aagtgatgtt cgttgactta ggtggcatct gtttagaaga ttcagtattg attggtcctc
     5701 gtgtcaacct catcacggtg aaccatctaa ccgatccaaa agaacgaaga ggcttgtcag
     5761 taaagccaat ccacatcaag aaaaatgctt ggatcggagc aggagcaact attctgccag
     5821 gcgtaacgat tggcgagaat gccattgttg cagcgaatgc aactgttacg aaagatgtcc
     5881 cagacaatac catcgttgca ggaatccctg ccaaaatcgt caaacctgtc gaacggatta
     5941 tttgaacagt aagatgaaat aagagatagt gagtataagc aagagatttt tcaaaacagg
     6001 aggagaatag aatgaatgat ttatcagaaa aagtgattgt catcatgggc gcttcaagcg
     6061 gaatcggtga agcaacaacg aagctattag cagaaaaagg ggctaaacta gttatagctg
     6121 cacgccgaga agatcgccta aaagcaataa aagaatcttt accagaagct gagctatata
     6181 ttcaaacagc agatgtaaga gattttgcac aagtccaagc agttatcgat ttagcgatgg
     6241 aaaagttcgg tcgaatcgat gtattgtaca ataatgcagg aatcatgcca accgctccat
     6301 tagttgaagg tcaccgtgat gaatggcaaa atatgcttga tatcaacatc atgggtgtgt
     6361 taaatggaat ttccgccgta cttccaatca tggaaaaaca aaaatctggg catatcattt
     6421 caactgattc tgtggcggga catgttgttt atcctgattc cgccgtttat tgcggaacaa
     6481 aatttgctgt ccgtgcaatc atggaaggat tacgtcaaga gcaacggcaa aataacatca
     6541 aatctacgat catctctcca ggagctgttc aaactgaatt atatcaaacc atcagcgaca
     6601 aaaaggctgc tcttgaactt catgaagcgc aaaaagaatg gggattgacc tccgaagata
     6661 tagcttctgc tgtagcattt gcgatagaaa cgccagaccg gatgtctgtc agtaacatga
     6721 ttatccgtcc gacgacacag gaagtataat ccataacgga taatatccaa agagaattac
     6781 ttgttaaata gttattcaaa cgaccatcaa tttaaggcgt gaatggagaa gaatattctt
     6841 catttacgtc tttttatgta gaaaaagcaa atttatcaaa aagaaacaaa tgttatttaa
     6901 atatggacat tgacttgctt ttactttttt agttacactg ttatttagga aaagtttcat
     6961 aattcaaagg agaaaaaaga tgaaagcaaa gaatttatta tttagcgtta ttcttttagg
     7021 aaatctagca gttgccaaca caacagtgct agctgaagaa tcgacagaaa ctgtcgcaac
     7081 tcagacatcg agttcttcag aaacaggaat tgaaaacgat acagagagta ctttgcagtc
     7141 agctactgtc tcagaaaata caatttcaac cgaagaacca acaactgaaa caacagaaca
     7201 aacacaaacc gaagacagta catcagcaga agctcagaac aatccagaga atgcagaaga
     7261 aatcacgaac caattgacag atgggcaaac attgacacat acccatcaag cagatttgtc
     7321 tgaattcgaa aactatactg gctatgcttt ttatgctcgc tcatctgcta attctcaaca
     7381 agcgttcatt gatagtattg cctcaactgc acaaagtttg gcaagtgcca atgacttata
     7441 tgcatcagtc atgattgctc aagccatcgt tgaaagtggc tgggggaaca gtactttggc
     7501 ttctgcccct aattacaatc tatttgggat taaaggaagc tataatggcc aatctgttac
     7561 gatgcctact tcggaatatg tgaatgggca atggattacg gtgaatgccg cttttagaaa
     7621 atatccttcg tacaaagaat cattgcaaga taacgtaacg gtattgaaga cgacttcttt
     7681 tcagccaggc gtttactact attccggtgc atggaaaagc aatacgaact cttacaaaga
     7741 tgctacggct tggctaacag gtcgttatgc cactgcgcct aattatggtt ctacattgaa
     7801 caatgtaatc gaaacctata atctgacaca gtatgataca gcaccaactt caacttctcc
     7861 gatgtatcgt ctgtataacc gtcatacagg agaacattta tatacattga atgccggaga
     7921 aaaaaattat ctgccaactg taggctggga atatgaaggg attgcttggc aagcaccaaa
     7981 cagcggacaa cctgtctacc gtttatacaa tccttacagt ggagaccatc attacacaat
     8041 ggcacaatct gagatcaact ctttaacaaa aatcggttgg cgttatgaag gtctaagttt
     8101 ttattctggt ggttccaagc caatttatcg actatttaat ccaaatgaaa aaactggtac
     8161 ccatcactat accttgagtg ctaaggaaag agattggcta acaccgatgg gctggagata
     8221 tgaaggaata ggattttatg gttattaaaa agaggtagtg aaaaagctgc cctgcatcaa
     8281 gtagtaagaa cagccaaaca aaaacagatt ttcatgtttt ttgtttggtt gaacttatta
     8341 ccgcagatgc aagtttttga acaccgttta ctcaaaaaag gttgtgacaa catcttgtca
     8401 taactttttt attactgttt aagcatactg atccaatttc taatattcga cgggattctt
     8461 gcaaaaggcg atttcggaaa tttttggcgg tgtaaaattt tctactgcag aagagctatc
     8521 ttctatagtt tgataagaat caataatctt ggttttagcg ttgtgctttc tttatcattt
     8581 tttccgttca ttctacaaaa aatttctttt ctctatactt ttctattgag agcgtttttt
     8641 ttattacaca gctgattctt tgactttttt ctttttttat tctattattg acaagtcaat
     8701 cgttgacgag tcaattatta gaaaggagga aattatgtct ttagaagaga ttagtcacct
     8761 tttatatcaa atcaaattag ccgatcaaaa aatcgtccag atctttgaag aaaatatcgg
     8821 tataagtctt actagatacg agatcatgat gagactgaaa gaagaaaatc cattgctaca
     8881 aagcagtttg caagaaaagt tgcagattga tcaaggagca gttactcgtc atctgaaggt
     8941 attggaagca aatggctatg tttcgcgtca gagaaatcct cagaacaacc gggaagtgta
     9001 tgtagcgcta acagaaaaag cccagaagaa aatcgagcat tgtgaaatgg accataaaga
     9061 tacaggttcc gtgctatccc cggctttcag cgatgtagaa atgaaacaat ttctaacact
     9121 tttagataaa ttgaataccc aactttgcaa tataacaaca gaaacgaaag aggttaataa
     9181 taatggaaca aaaaaatgat ttttcaacaa ttatgactgg tagaagatcc gttcgcgtat
     9241 atgatgaaac atacaaaatc ccacacgaag aaatgctgga aatgatcaaa gaagcgacga
     9301 ctgcaccttc ttctgtcaat atgcaaccat ggcgctttgt ggtcgctgaa agcgaggaag
     9361 caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag acaaaatgat acttcttctg
     9421 caatggtcat gatttttggc gatatgcaat gttacgaata tggcgaagaa atctataacc
     9481 aagcttacga atcagggaag atgccaaaag aagtcagaga tcaacaatta gctgcgatca
     9541 ttccttatta caagagtttt tcaagagaag aaatgaacga tgtagtcaaa gtcgattcta
     9601 gcttagcggc tatgcaattc atgttagttg cgcgtgatca tggctatgaa acaaatccaa
     9661 ttggtggatt cgaagcagat cagttggcag aggcatttgg tttggataaa gaccgttatg
     9721 ttccagttat tattttatca ataggaaaag cggtagaaga aggttacgag tctgttcgtt
     9781 tagcaccaga aaagatcact acgttcgaat aaaaggtttt cagttactat tcagtagcta
     9841 ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag gaggtcggaa aaatgactgc
     9901 atcgatgaaa ttacgtttcg aacttttgaa taacaactaa ataaattcaa aagacatgta
     9961 taaacaacaa tttcatggat ggagtatgtc cattttttcg aacgacagta gaataggtga
    10021 aagctatgac agatttggtg atttagtcat agttatttcg ttcagagtag gaaaagatgt
    10081 gatgcagttt ttttgcaaac agatttttct gctctttttt agtatcgata attgttgttt
    10141 tacatgaaat aggagtgtaa aacatatgga aaatttagca gtaaatataa caaatctaca
    10201 agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct atcagcaaga
    10261 tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca aactaatagc
    10321 cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca actacctgcc
    10381 tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca gtcatttcca
    10441 gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac gaaagatcga
    10501 gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc caacaaccca
    10561 tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt atggtacgct
    10621 ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa tctgggaagt
    10681 aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca ctcaaaagga
    10741 attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga aaaaacgatt
    10801 ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag tgtccagtaa
    10861 aaagaaaaag caacaaatca gaccggatcg gttgtcttcc tctaaacaaa aagacagtgt
    10921 acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa aaataggaga
    10981 aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat ctcttgaact
    11041 ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg ggagaacatt
    11101 actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg tcggtgaaaa
    11161 tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag gaatccttct
    11221 ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga catctgaaga
    11281 atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg tccgtagttt
    11341 gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta cattaagtgg
    11401 gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta atgtgttgct
    11461 gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag agaagctaat
    11521 gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg tcgagcgtac
    11581 ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta cgagaaattt
    11641 ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga ttgcctacaa
    11701 ataaaaaaga aggaataatt tttacaacaa tgatgtgttt tttgatggtc ttggggatga
    11761 gcgcgtataa tttatggctg catcaagatc ttcatttatc aagtttgttg attggtctag
    11821 tgcctggatt cgtggtggct ttcattttgg atgtcttcat catcggtgtg ctggcgaaaa
    11881 aaattgcctt taaactacca gtaaatccag aaagcaaggt ccaaatgatc ttaactattt
    11941 catgcttgat ggttttagga atggtgacat gtatgtcttt attcggtgtg atcatggaac
    12001 aaggaatacc tgatcaatta ttctctgctt atatgaatgc ttggaaaatg aatgtgattg
    12061 ccgctttacc gttgcagttg atcatcgttg gcccgctttc aagaagtgtg ctaaaagcaa
    12121 ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa ggccttggca tgctttttgt
    12181 catagtctct tgaatgcagt tctagaaaaa tagtgatccg aacgataggt tttggcctgt
    12241 cgttcggatc gctttttctt ttccaactgt attgctcttt tattgaagat aatttgctct
    12301 tcaatagata tttctagaaa ggttctgttt atcccttact caaaaaactg ctgcatttgt
    12361 tctcttactt gctgacacca ttggagttcc tgctgacaaa gcataattct gcgctggatc
    12421 acaacataac gtccaaattc ttgtttcttg tctagcgaga ggtcattcaa ttttttttga
    12481 tttttttgca tgattttgtt cagctgttgt tctctagtga atagcagtcc agtagctgtt
    12541 gttttatcga gcgtcgaaaa agcatagatt tttgctagaa attcatcttt ttgcgtagga
    12601 gcgggagttt cctctttgat ccattcctct acaagagcac gtccttcatc agtcaagtgg
    12661 tagatctttt tttgagagtg ctcgtctttg tccaaaacgg tcacataacc ctgctggtct
    12721 aacttggcaa gcgttgtata gatctgactg tgatgggctt cccaaaaaat tgtgatcagc
    12781 tgcttcaatt catacccaga acgtggtttg gataaaagca cgcataataa tatataggct
    12841 aaagtattca taaaaatcct acctctcgtc ttattttctt tattatatca cagtaaagtt
    12901 tccacgtttt acatgtgcat atttacatgt aaaataaatc atgtatgatg ctaatagaga
    12961 gaaagggatg gaaatattgg agaatcaaat agatatacaa gaacaaacaa atgaagaaac
    13021 aaaggggaaa agttcagcgc tagttgtagc agctacctgt atgatcgctt ttatgggagt
    13081 gggtctagta gacccaatct taaaaacgat tgctgcacag ttaaacgcta cgccagctga
    13141 aacgaccctg ttatttacta gttacatgtt agtcactggt gtggtgatgc tatttacagg
    13201 tttcttatct agtcggatcg gtttgaaaaa acattgatga caggattgtt tatcatcgtt
    13261 gtattcgctg gtcttggcgg attatctggg aatatcggaa tgctgattgg gttacgtgcc
    13321 ggctggggtg tcgggaatgc tttatttgtc tctactgctt tagcaacgat cgtcagtatt
    13381 ttagttggta acacagaaaa agcaatcatg atgtatgaag cagcacttgg ctgcggaatg
    13441 gctgttggcc ctctagtcgg tggggtcttg ggcagtggtt catggcgtta tccatttttt
    13501 ggggtagcat ttttgatgtt ttgcggtttt atagccctag cggtactttt acctgaaact
    13561 gagaaaccaa agagaaaagt aggcctttgg gaaggtgtca aagcgttaag caatcataaa
    13621 cttcgttcag ttggactaat cgcattgtta tataatttcg gattctttac tttacttgcg
    13681 tatgcgccat ttttattgat tggctattct gaattagaag tcggttttgt attttttggc
    13741 tggggtgtgc ttctggctat tgcttcgatt ttcgttgcac cggctcttga gcggaagttt
    13801 acgacttatc ggacaatttt agcagcattt gtcttattca ttatttgttt gctgttatta
    13861 ggagctggaa cttctatgcc agtccttgta ccgatcagca ttgtgttagc aggatttttc
    13921 caaggaatta tcaatacact gttgacgacg attgctatgg aaataccagg tctgcaacga
    13981 aacgttgctt cgtccagcta tagttttgtc cgctttttcg gtggagcttt agcaccattt
    14041 attgcaggtg tgattggtga acatattggt gaaagctatg cgttttattt tgcaggtgcc
    14101 attgttctta tcagtgtatg ttgtatcatt tatcaccgta attattttgc tacaaatgaa
    14161 gaagaataag aaatcagagg ttgtgaaaaa gctgtcctgc atcaagtcat aagaa
//
LOCUS       DAFCNS010000260         7959 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.100, whole genome shotgun sequence.
ACCESSION   DAFCNS010000260 DAFCNS010000000
VERSION     DAFCNS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.100"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            128..1564
                     /locus_tag="LNE23_003002"
     CDS             128..1564
                     /locus_tag="LNE23_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6932770.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="LNE23_003003"
     CDS             1753..4200
                     /locus_tag="LNE23_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK6932771.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="LNE23_003004"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="LNE23_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBK6932772.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="LNE23_003005"
     CDS             complement(5690..6793)
                     /locus_tag="LNE23_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6932773.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="LNE23_003006"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="LNE23_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK6932774.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2664 a         1359 c         1729 g         2207 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAFCNS010000261         5822 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.116, whole genome shotgun sequence.
ACCESSION   DAFCNS010000261 DAFCNS010000000
VERSION     DAFCNS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.116"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..998)
                     /locus_tag="LNE23_003007"
                     /pseudo
     CDS             complement(1..998)
                     /locus_tag="LNE23_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            1489..2427
                     /locus_tag="LNE23_003008"
     CDS             1489..2427
                     /locus_tag="LNE23_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6932775.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2557..3204
                     /locus_tag="LNE23_003009"
     CDS             2557..3204
                     /locus_tag="LNE23_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HBK6932776.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(3897..4835)
                     /locus_tag="LNE23_003010"
                     /pseudo
     CDS             complement(3897..4835)
                     /locus_tag="LNE23_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4835..5452)
                     /locus_tag="LNE23_003011"
     CDS             complement(4835..5452)
                     /locus_tag="LNE23_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6932777.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(5466..5636)
                     /locus_tag="LNE23_003012"
     CDS             complement(5466..5636)
                     /locus_tag="LNE23_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932778.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
BASE COUNT         1846 a          957 c          946 g         2073 t
ORIGIN      
        1 aatacaatat tgttgccaca cttaggacat tttcctacaa tacttttttc tgcttctttt
       61 tctttctgtt cctggtaatc agaaaaattt agtttttcta tatcgttagg tacagcttcc
      121 agtaaatgaa caatgaattt tttgatattc gtaataaagt tctcttgatt gccttctctt
      181 ttaccgattt tttttaaata cgtttcccat ttagccgtca tttcagcact cgttaaaagg
      241 tgctgacttt caactgcctg gcacaataat tttccttttt cagttacaac aagcttattc
      301 ttaatcactt ggatatattc tttttgtttt aacgcttcaa taatacttgc tcttgtcgct
      361 tctgttccaa tcccctcaac ttcttgtaaa atcttgattg cttcttcatc atcaaccgtt
      421 ttattcgccg ttttcatagc agttaataat gtaccctctg taaaagcttt tggcggttgc
      481 gtttcttttt caacactctt aacaccaact tcggcacgtt cgccaatgat aacgagtggt
      541 aacgtttgaa cgtcctcttt ttcttctttg gtttgttgtt tgaaaagaat tttccaccct
      601 tcttgctttg gtgtctttcc gatcgattga aacagcaaat cagctacttt cgtttgtatc
      661 ttggtttctt catacaagta atcaggtaaa aacatggcaa cggtcgtttt aacgactaaa
      721 gcataaattt ttcgttgtaa atcgtccatt ttcgctaatg cagattcggt aggtacttgt
      781 tttgttggga taatggcgtg gtgttcctgt accttactac cgtccacata acgctttcta
      841 ggctctgttt gaaccatttc aagatcaagt cctaaaaaac cgctatattt gccaaaattc
      901 gcttttaaat aagcaaattc gttctctgta ataaatggtg tatctgttct tggataactc
      961 aataattttg cttcatacag tccttgcata gcttttaaac gtaaaatttt gcgttctaag
     1021 acgttttaaa tatatctgcg tatattttta tgtgttttat cttttcgtcg gcaaatgcgt
     1081 taaaatttgc gaaataaaat agctcaaggg cgaacgcagt gagtgcattt acccttgatc
     1141 tatttttttg agtaaattag cttttgaaac cgacggaaag gaaacacaaa acttttcaga
     1201 ggcaaggggt ttttaagggg cgcttttagc cccttgaatt tatttgcttt aagatatatt
     1261 tagttttaaa atagttttaa cgaaaaaaag acataaaaaa agacgagcaa cgctaccaac
     1321 gttctcgccc ggaataacaa accacacttg ttattcattc gatgattagt atatcaattt
     1381 ttgattttga atacaaaaca aaatggacga atggatacag gcgaaattta ttttgcgatt
     1441 ccactcgtcc ttttttcgtt ccaaaaatta ttttaggagc gataaaaaat gaagaaaatt
     1501 gatgaaaaat tagctgcaga aatctcacta aaaaaaccgg ccgtctctaa tagccggtta
     1561 agtaatagcc ggttaagtgg tcaaactttg ggaaaatctc aacccgcatt aagttttgat
     1621 gccatgacaa tcgttggaaa tttgaacaaa actaatgcta aaaagctatc tgactttatg
     1681 agtgtagagc cacaaatacg actttgggat atacttcaaa caaagtttaa agctaaggca
     1741 cttcaagaaa aagtttatat cgaatatgac aaagtaaaag cagatacttg ggatagacgt
     1801 aatatgcgtg ttgaatttaa tcccaataaa ctcacacatg aagaaatgat ttggttaaaa
     1861 caaaatatta tcgactacat ggaagatgac ggttttacaa gattagactt agcttttgat
     1921 tttgaagatg atttgagcga ttactatgca atgactgata aagcagttaa gaaaactgtt
     1981 ttttatggtc gtaatggcaa gccagaaaca aaatattttg gtgtccgtga tagtgataga
     2041 tttattagaa tttataataa aaaacaagaa cgtaaagata acgcagatgt tgaagttgtg
     2101 tctgaacatt tatggcgtgt agaagttgaa ttaaaaagag atatggttga ttactggaat
     2161 gattgtttta atgatttaca catcttgaaa cctgcgtggg ctactttaga aaaaattaat
     2221 gagcaagcta tggtttatac tttgttgcat gaagaaagta tgtggggaaa gctaagtaag
     2281 aatactaaga ctaaatttaa aaaattgatt agagaaatat ctccaattga tttaacggaa
     2341 ttaatgaaat cgactttaaa agcgaacgaa aaacaattgc aaaagcagat tgatttttgg
     2401 caacgtgaat ttaggttttg gaagtaaaat aagttttatt tgataaaaat tgctaattca
     2461 gtataattaa tatttacgag gtgacataac gtatgaaaaa atcagaggat tattcctcct
     2521 aaatataaaa atttaaaatt taggaggaag ttatatatga cttttaatat tattgaatta
     2581 gaaaattggg atagaaaaga atattttgaa cactatttta atcagcaaac tacttatagc
     2641 attactaaag aaattgatat tactttgttt aaagatatga taaaaaagaa aggatatgaa
     2701 atttatccct ctttaattta tgcaattatg gaagttgtaa ataaaaataa agtgtttaga
     2761 acaggaatta atagtgagaa taaattaggt tattgggata agttaaatcc tttgtataca
     2821 gtttttaata agcaaactga aaaatttact aacatttgga ctgaatctga taaaaacttc
     2881 atttcttttt ataataatta taaaaatgac ttgcttgaat ataaagataa agaagaaatg
     2941 tttcctaaaa aaccgatacc tgaaaacacc ataccgattt caatgattcc ttggattgat
     3001 tttagttcat ttaatttaaa tattggtaac aatagcagct ttttattgcc tattattacg
     3061 ataggtaaat tttatagtga gaataataaa atttatatac cagttgctct gcaacttcat
     3121 cattctgtat gtgatggtta ccatgcttca ctatttatga atgaatttca agatataatt
     3181 catagggtag atgattggat ttagttttta gattttgaaa gtgaatttaa ttttatacac
     3241 gtaagtgatc ataaaattta tgaacgtata acaaccacat tttttggttg cttgtggttt
     3301 tgattttgaa tttggttttg aacttatgga ctgatttatt cagtccattt tttgtgcttg
     3361 cacaaaaact agcctcgcag agcacacgca ttaatgactt atgaaacgta gtaaataagt
     3421 ctagtgtgtt atactttact tggaagatgc accgaataaa aaatattgaa gaacaactag
     3481 caaaagattt taaagagtta ttttatttta agtctttata acatgagtga agcgaatttt
     3541 taaatttcga tagaaatttt tacatcaaaa agccccctgt caaaattgac gaagggggtt
     3601 ttttggcgca cgcttttcgt tagaaatata caagattgaa aatcgtgtat aagtgcgccc
     3661 tttgttttga acttagcacg ttacatcaat tttttaaaat gatgtataag tgcgcccttt
     3721 taaattttga gtgattatat tttttgagtt agaaaaaggg attgggaaaa tttcccaaaa
     3781 taatttaaaa aataagcaaa aattttcgat agagaatgtg ctattttttg tcaaaggtgt
     3841 ataccttgac tgtgcttgct gttacattaa gtttattttt aagttattaa aaaagaaata
     3901 gcttttaaag tttggctcgc tgtcgcttta taaagctgat tgacttttga ttgcaaacta
     3961 cttaaagaaa acaaactcgg actattcgtt ttcttctctt tggtttgaac atcagcaatt
     4021 atcccctctt gattgcctat tttagcttgt ttagaagaaa caaaagctaa aagctcctct
     4081 tgggttttaa aacgctgtgt ggggcttaga acgcccttaa acgacccttg gtttactttt
     4141 atactagctt ccacctcgaa aaaaggttct tttttaaaat tctctatggc ttcctggcgc
     4201 tgaaaaataa ggtataaggt gggcgtttga acacgtccta gtgaaaatgt accttgtacg
     4261 cccttctgtt gtaaatttaa cgtatacaaa gggcttgcgt tcatgccgat caaccaatcg
     4321 gcaatttggc gtgtttgcgc ttcttgataa aagggatagt aattcattcc aggttgcaaa
     4381 ttttgaaaac cgcttcggat tacatctttt tctaagctat tgatccatag tcttttaaat
     4441 gttttatctt ttgaaaaggc atttgcttta tggataatcg accaggcgat attttcacct
     4501 tctctgtcgc tatctgttgc aacaataatt gtatttgcct ttttgagaag ttctgcaaca
     4561 attttaaact gctttccctt atcttttgca acttcaaaat cgtatcgatc aggaaaaatc
     4621 ggcaaagatt caagtttcca attttgccac ttttcgtcat aatgacctgg ttctgctaat
     4681 tccactaaat gcccaaaacc aaaggtgata aacgtttcat ctgtaaatag tgggtctttg
     4741 atctcaaaat aaccgtcttt tttggtgctt tgttttaaag cacttgcgta ggctaatgcc
     4801 tggcttggtt tttcagctaa aataaccgta ctcattaact atccctcttt tcattgtttt
     4861 ttctttgatc gactgtcacg ttatatcttg ctcgatacct tctaaacgtt cggcgattga
     4921 ttccagtttg ttcttcaact tctttatcgg ataaaccatt caaaaacaaa tcgaaagcat
     4981 gctttaaacg tggatcattt tctttaaatt tatgctgacg acctttgaat ttgccttttt
     5041 tcttagcaat ttcgattcct tgtgcctgac gttccttaat tttttttcgt tctgattctg
     5101 cttgatactt gtacaattca atgacaaggc tattaatcaa acgccttaaa ttttcatctt
     5161 caataccatt cattgagggt aaatttaaga cttccagggt tgccccctta atttgaattt
     5221 gattcatcaa ttctgttaat tctttattat ttcgtcctaa tcgatctaat tcagtaacaa
     5281 taacaatatc cccttcacga atatagttaa gcatagcttg taattgtggg cgttcgaccg
     5341 attgaccgct taatttgtct gaaaagacct tagaaacgcc ctgtaacgct tgtaattgcc
     5401 gatctaagtt ctgttctttg ctactgacac gtgcataacc aattttagcc attttcaacc
     5461 aacctctaaa attctctcgg ttgcaataac caatcagcaa tatctacttt ttcaatttca
     5521 aattgcttat cagaaattgt cttttcgtaa gcgataaaat cttgcgcata ttgttgctca
     5581 ttaaaaatag ccaccacttc gtcattttct aaaactcgat aaataaattt tttcatttta
     5641 ctcctcctat tatgcccaac ttaaatgacc tattcaccaa gtcaattata ctgctaaaat
     5701 catattagga caaataggta tactctaatg acctataaaa gatagaaaat taaaaaatca
     5761 agtgttcgct tcgctctcac tgcccctcga cgttttagta gcctttccct cacttcgttc
     5821 ag
//
LOCUS       DAFCNS010000262         4804 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.124, whole genome shotgun sequence.
ACCESSION   DAFCNS010000262 DAFCNS010000000
VERSION     DAFCNS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.124"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            92..787
                     /gene="vanR"
                     /locus_tag="LNE23_003013"
     CDS             92..787
                     /gene="vanR"
                     /locus_tag="LNE23_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK6932779.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            765..1919
                     /locus_tag="LNE23_003014"
     CDS             765..1919
                     /locus_tag="LNE23_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBK6932780.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2134..3102
                     /locus_tag="LNE23_003015"
     CDS             2134..3102
                     /locus_tag="LNE23_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK6932781.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3095..4126
                     /gene="vanA"
                     /locus_tag="LNE23_003016"
     CDS             3095..4126
                     /gene="vanA"
                     /locus_tag="LNE23_003016"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK6932782.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4132..4740
                     /locus_tag="LNE23_003017"
     CDS             4132..4740
                     /locus_tag="LNE23_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBK6932783.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          922 c         1119 g         1281 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      841 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      901 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
      961 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1021 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1081 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1141 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1201 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1261 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1321 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1381 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1441 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1501 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1561 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1621 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1681 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1741 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1801 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1861 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1921 agatgtatat aattttttag gaaaatctca aggttatctt tactttttct taggaaatta
     1981 acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga
     2041 gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt
     2101 tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat
     2161 ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg
     2221 gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa
     2281 tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga
     2341 gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct
     2401 gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat
     2461 tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg
     2521 gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt
     2581 ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga
     2641 tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat
     2701 gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac
     2761 tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact
     2821 gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg
     2881 ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc
     2941 caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc
     3001 acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt
     3061 aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact
     3121 gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc
     3181 taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg
     3241 gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact
     3301 ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa
     3361 ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca
     3421 aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat
     3481 ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc
     3541 cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt
     3601 tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga
     3661 attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc
     3721 tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg
     3781 cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc
     3841 ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg
     3901 aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc
     3961 ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct
     4021 gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact
     4081 tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata
     4141 ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact
     4201 tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca
     4261 tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga
     4321 ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat gcaatgggct
     4381 gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag
     4441 atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat
     4501 cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt
     4561 atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc
     4621 agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg
     4681 cactatgtat taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa
     4741 acttttaacc gttgcacgga caaacggttc tgttgcaaag ttttaaataa agaataaaat
     4801 ccct
//
LOCUS       DAFCNS010000263         4757 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.125, whole genome shotgun sequence.
ACCESSION   DAFCNS010000263 DAFCNS010000000
VERSION     DAFCNS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.125"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            257..1168
                     /locus_tag="LNE23_003018"
     CDS             257..1168
                     /locus_tag="LNE23_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBK6932784.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1321..1806
                     /gene="vanZ"
                     /locus_tag="LNE23_003019"
     CDS             1321..1806
                     /gene="vanZ"
                     /locus_tag="LNE23_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBK6932785.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2325..2609
                     /locus_tag="LNE23_003020"
     CDS             2325..2609
                     /locus_tag="LNE23_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6932786.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2975..3274
                     /locus_tag="LNE23_003021"
     CDS             2975..3274
                     /locus_tag="LNE23_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932787.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            3317..>4757
                     /locus_tag="LNE23_003022"
     CDS             3317..>4757
                     /locus_tag="LNE23_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBK6932788.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAE"
BASE COUNT         1768 a          691 c          877 g         1421 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccccca ttgtcaacag gcagttcagc
       61 ctcgttaaat tcagcatggg tatcacttat gaaaattcat ctacattggt gataatagta
      121 aatccagtag ggcgaaataa ttgactgtaa tttacggggc aaaacggcac aatctcaaac
      181 gagattgtgc cgtttaaggg gaagattcta gaaatatttc atacttccaa ctatatagtt
      241 aaggaggaga ctgaaaatga agaagttgtt ttttttattg ttattgttat tcttaatata
      301 cttaggttat gactacgtta atgaagcact gttttctcag gaaaaagtcg aatttcaaaa
      361 ttatgatcaa aatcccaaag aacatttaga aaatagtggg acttctgaaa atacccaaga
      421 gaaaacaatt acagaagaac aggtttatca aggaaatctg ctattaatca atagtaaata
      481 tcctgttcgc caagaaagtg tgaagtcaga tatcgtgaat ttatctaaac atgacgaatt
      541 aataaatgga tacgggttgc ttgatagtaa tatttatatg tcaaaagaaa tagcacaaaa
      601 attttcagag atggtcaatg atgctgtaaa gggtggcgtt agtcatttta ttattaatag
      661 tggctatcga gactttgatg agcaaagtgt gctttaccaa gaaatggggg ctgagtatgc
      721 cttaccagca ggttatagtg agcataattc aggtttatca ctagatgtag gatcaagctt
      781 gacgaaaatg gaacgagccc ctgaaggaaa gtggatagaa gaaaatgctt ggaaatacgg
      841 gttcatttta cgttatccag aggacaaaac agagttaaca ggaattcaat atgaaccatg
      901 gcatattcgc tatgttggtt taccacatag tgcgattatg aaagaaaaga atttcgttct
      961 cgaggaatat atggattacc taaaagaaga aaaaaccatt tctgttagtg taaatgggga
     1021 aaaatatgag atcttttatt atcctgttac taaaaatacc accattcatg tgccgactaa
     1081 tcttcgttat gagatatcag gaaacaatat agacggtgta attgtgacag tgtttcccgg
     1141 atcaacacat actaattcaa ggaggtaagg atggcggaat gaaaccaacg aaattaatga
     1201 acagcattat tgtactagca cttttggggt aacgttagct ttttaattta aaacccacgt
     1261 taactaggac attgctatac taatgataca acttaaacaa aagaattaga ggaaattata
     1321 ttgggaaaaa tattatctag aggattgcta gctttatatt tagtgacact aatctggtta
     1381 gtgttattca aattacaata caatatttta tcagtattta attatcatca aagaagtctt
     1441 aacttgactc catttactgc tactgggaat ttcagagaga tgatagataa tgttataatc
     1501 tttattccat ttggcttgct tttgaatgtc aattttaaag aaatcggatt tttacctaag
     1561 tttgcttttg tactggtttt aagtcttact tttgaaataa ttcaatttat cttcgctatt
     1621 ggagcgacag acataacaga tgtaattaca aatactgttg gaggctttct tggactgaaa
     1681 ttatatggtt taagcaataa gcatatgaat caaaaaaaat tagacagagt tattattttt
     1741 gtaggtatac ttttgctcgt attattgctc gtttaccgta cccatttaag aataaattac
     1801 gtgtaagatg tctaaatcaa gcaatctgat ctttcataca cataaagata ttgaatgaat
     1861 tggattagat ggaaaacggg atgtggggaa actcgcccgt aggtgtgaag tgaggggaaa
     1921 accggtgata aagtaaaaag cttacctaac actatagtaa caaagaaagc ccaattatca
     1981 attttagtgc tgaggaattg gtctctttaa taaatttcct taacgttgta aatccgcatt
     2041 ttcctgacgg tacccccact agcagccatg ctttttttga atctttgtag gacgttttaa
     2101 agagaattac ccactttaaa attaaacatg cgcttagaat cgctttagga aacacgatcc
     2161 agtccaataa tcgtcgataa aaacttttga aaaaggttgg tgaaattacc tacttttgga
     2221 ataatcacaa atcacaagtg attaatcact tgtttattaa gatattaaaa gctataattt
     2281 aaataaagcg tgaattttat tacacaaaaa gaggggggag aaacttggaa ctagcattta
     2341 gagaaagctt aaaaaagatg agaggtacca aatcaaaaga aaaattctcc caagaattag
     2401 aaatgagtag atcaaattat tcacgaatag aatcaggaaa atcagatcca accataaaaa
     2461 cactagaaca aattgcaaag ttaactaact caacgctagt agtggattta atcccaaatg
     2521 agccaacaga accagaacca gaaacagaat cagaacaagt aacattggat ttagaaatgg
     2581 aagaagaaaa aagcaatgac ttcgtgtgaa taatgcacga aatcgttgct tatttttttt
     2641 aaaagcggta tactagatat aacgaaacaa cgaactgaat agaaacgaaa aaagagccat
     2701 gacacattta taaaatgttt gacgacattt tataaatgca tagcccgata agattgccaa
     2761 accaacgctt atcagttagt cagatgaact cttccctcgt aagaagttat ttaattaact
     2821 ttgtttgaag acggtatata accgtactat cattatatag ggaaatcaga gagttttcaa
     2881 gtatctaagc tactgaattt aagaattgtt aagcaatcaa tcggaaatcg tttgattgct
     2941 ttttttgtat tcatttatag aaggtggagt ttgtatgaat catgatgaat gtaaaactta
     3001 tataaaaaat agtttattgg agataagaaa attagcaaat atctatacac tagaaacgtt
     3061 taagaaagag ttagaaaaga gaaatatcta cttagaaaca aaatcagata agtatttttc
     3121 ttcggagggg gaagattata tatataagtt aatagaaaat aacaaaataa tttattcgat
     3181 tagtggaaaa aaattgactt ataaaggaaa aaaatctttt tcaaaacatg caatattgaa
     3241 acagttgaat gaaaaagcaa accaagttaa ttaaacaacc tattttatag gatttatagg
     3301 aaaggagaac agctgaatga atatcccttt tgttgtagaa actgtgcttc atgacggctt
     3361 gttaaagtac aaatttaaaa atagtaaaat tcgctcaatc actaccaagc caggtaaaag
     3421 caaaggggct atttttgcgt atcgctcaaa atcaagcatg attggcggtc gtggtgttgt
     3481 tctgacttcc gaggaagcga ttcaagaaaa tcaagataca tttacacatt ggacacccaa
     3541 cgtttatcgt tatggaacgt atgcagacga aaaccgttca tacacgaaag gacattctga
     3601 aaacaattta agacaaatca ataccttctt tattgatttt gatattcaca cggcaaaaga
     3661 aactatttca gcaagcgata ttttaacaac cgctattgat ttaggtttta tgcctactat
     3721 gattatcaaa tctgataaag gttatcaagc atattttgtt ttagaaacgc cagtctatgt
     3781 gacttcaaaa tcagaattta aatctgtcaa agcagccaaa ataatttcgc aaaatatccg
     3841 agaatatttt ggaaagtctt tgccagttga tctaacgtgt aatcattttg gtattgctcg
     3901 cataccaaga acggacaatg tagaattttt tgatcctaat taccgttatt ctttcaaaga
     3961 atggcaagat tggtctttca aacaaacaga taataagggc tttactcgtt caagtctaac
     4021 ggttttaagc ggtacagaag gcaaaaaaca agtagatgaa ccctggttta atctcttatt
     4081 gcacgaaacg aaattttcag gagaaaaggg tttaataggg cgtaataacg tcatgtttac
     4141 cctctcttta gcctacttta gttcaggcta ttcaatcgaa acgtgcgaat ataatatgtt
     4201 tgagtttaat aatcgattag atcaaccctt agaagaaaaa gaagtaatca aaattgttag
     4261 aagtgcctat tcagaaaact atcaaggggc taatagggaa tacattacca ttctttgcaa
     4321 agcttgggta tcaagtgatt taaccagtaa agatttattt gtccgtcaag ggtggtttaa
     4381 attcaagaaa aaaagaagcg aacgtcaacg tgttcatttg tcagaatgga aagaagattt
     4441 aatggcttat attagcgaaa aaagcgatgt atacaagcct tatttagtga cgaccaaaaa
     4501 agagattaga gaagtgctag gcattcctga acggacatta gataaattgc tgaaggtact
     4561 gaaggcgaat caggaaattt tctttaagat taaaccagga agaaatggtg gcattcaact
     4621 tgctagtgtt aaatcattgt tgctatcgat cattaaagta aaaaaagaag aaaaagaaag
     4681 ctatataaag gcgctgacaa attcttttga cttagagcat acattcattc aagagacttt
     4741 aaacaagcta gcagaac
//
LOCUS       DAFCNS010000264         4630 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.129, whole genome shotgun sequence.
ACCESSION   DAFCNS010000264 DAFCNS010000000
VERSION     DAFCNS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.129"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..212
                     /locus_tag="LNE23_003023"
     CDS             <1..212
                     /locus_tag="LNE23_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK6932789.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
     gene            447..683
                     /locus_tag="LNE23_003024"
     CDS             447..683
                     /locus_tag="LNE23_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932790.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            690..2642
                     /locus_tag="LNE23_003025"
     CDS             690..2642
                     /locus_tag="LNE23_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBK6932791.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="LNE23_003026"
     CDS             complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="LNE23_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBK6932792.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3573..4613
                     /locus_tag="LNE23_003027"
     CDS             3573..4613
                     /locus_tag="LNE23_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK6932793.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
BASE COUNT         1612 a          750 c          833 g         1435 t
ORIGIN      
        1 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
       61 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      121 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      181 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
      241 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
      301 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
      361 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
      421 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
      481 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
      541 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
      601 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
      661 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
      721 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
      781 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
      841 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
      901 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
      961 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     1021 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     1081 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     1141 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     1201 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     1261 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     1321 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     1381 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     1441 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     1501 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     1561 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     1621 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     1681 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     1741 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     1801 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     1861 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     1921 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     1981 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     2041 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     2101 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     2161 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     2221 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     2281 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     2341 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     2401 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     2461 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     2521 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     2581 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     2641 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     2701 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     2761 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     2821 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     2881 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     2941 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     3001 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     3061 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     3121 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     3181 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     3241 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     3301 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     3361 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     3421 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     3481 acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     3541 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     3601 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     3661 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     3721 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     3781 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     3841 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     3901 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     3961 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     4021 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     4081 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     4141 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     4201 acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt actcctattg
     4261 tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat cttagtttta
     4321 aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc aaatattgtg
     4381 caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga gaaattcctg
     4441 ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg cttagtgttt
     4501 gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc cagacccgtc
     4561 gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact tagtgcgttt
     4621 tatgttgtta
//
LOCUS       DAFCNS010000265         3682 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.144, whole genome shotgun sequence.
ACCESSION   DAFCNS010000265 DAFCNS010000000
VERSION     DAFCNS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.144"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..226
                     /locus_tag="LNE23_003028"
     CDS             <1..226
                     /locus_tag="LNE23_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6932794.1"
                     /translation="FILYLKLCNRTLLVEDASLSGENERINELIEFIRKNKFRTLEYD
                     DKLVRKIIQSVTVYEGHFVIAFKSGIEMEI"
     gene            369..593
                     /locus_tag="LNE23_003029"
     CDS             369..593
                     /locus_tag="LNE23_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6932795.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            608..1477
                     /locus_tag="LNE23_003030"
     CDS             608..1477
                     /locus_tag="LNE23_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBK6932796.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1458..2192
                     /locus_tag="LNE23_003031"
     CDS             1458..2192
                     /locus_tag="LNE23_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6932797.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2225..3133
                     /locus_tag="LNE23_003032"
     CDS             2225..3133
                     /locus_tag="LNE23_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBK6932798.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3130..3610
                     /locus_tag="LNE23_003033"
                     /pseudo
     CDS             3130..3610
                     /locus_tag="LNE23_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1278 a          533 c          798 g         1073 t
ORIGIN      
        1 ttttattctt tatttaaaac tttgcaacag aaccctttta gtagaagatg cctccctcag
       61 tggcgagaac gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgcac
      121 cttagaatat gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaagg
      181 ccacttcgtc atagccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa
      241 tagcccatga ctctacagta gagttgtggg ttttcttttg cttgctgatt ataataatat
      301 tttaataact attgtgttta tcaaccaaat ggtttataat attcttagcg taaacttgga
      361 ggtgctgtat gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg
      421 tttccgaact tgctagacgt attggccaga ctccacagaa tttcaataaa aaattacaac
      481 gagaaacagt aaccttggat gagttgaagg ccattgctga tgtgctaggt gtcaagtttg
      541 tgcaggcatt tattttacca gatggagaag aaataaaaat atctaacgaa taaaggagga
      601 tgtttatatg gtaggtaatg ttattcaaat agtaacagag aaattatcat ctttgccttt
      661 tatagaaggc atcgtattag ggggctcacg tgcaagaggc acccatacag aaaattctga
      721 tatagatatc ggaatctatt acaattcaga ctcatttgac ctgacagcga ttaatcaaat
      781 tgctacagaa ttggatgata aaaatagaaa caaccttgtt gtgcctcccg gagcgtgggg
      841 tgattggatt aatggcggcg gatggttatt tataaatggg tatcatgttg acttgatttt
      901 acgtgatata aaacgggtag aacaaataat caaagatacg gagcaaggaa ttgttactgc
      961 caattatcag accgggcatc cccatggtta tataagtgca atgtatcgtg gagaattagc
     1021 gattagtaaa atactatatg ctaagaacga aagcttatgc gaattaaaaa agcaggcaga
     1081 aatttaccct actgctctaa agaaaagctt gatgaacttt tttatatttg aagctgaatt
     1141 ctctttaatg tttgtaaaag caaatgcggg ggtagaggat aaatattata ttgcaggcca
     1201 tgtttttcgt attatttcat gcttaaatca agtactattt gcatgtaata atgcttattg
     1261 cattaacgaa aagaaagcta taaaactgct tgaaactttt gaatataaac ctgaaaaata
     1321 tgccgaaaga gttaatcata tttttgaagt actcggtctt tctctttttg aatgctacga
     1381 tatgaccgag aaactttata aagaagtgaa tgaaattgta tcggagataa ataacttttt
     1441 aaacgaggag agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat
     1501 acagtcaaat gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga
     1561 aaaagatgct acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat
     1621 ggcactgtat atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc
     1681 ataaaatgct cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct
     1741 gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg
     1801 cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg
     1861 ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag
     1921 actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg
     1981 agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga
     2041 ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac
     2101 agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga
     2161 tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat
     2221 gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga
     2281 acgtattcga attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga
     2341 atttcaggat tatgatatta catattttgt aagtgatata gaaccgttta tatctaatga
     2401 tgactggctt aatcaatttg ggaatataat aatgatgcaa aagccggagg atatggaatt
     2461 attcccacct gaagaaaagg gattttccta tcttatgcta tttgatgatt acaataaaat
     2521 tgatcttacc ttattgccct tggaagagtt agataattac ctaaagggcg ataaattaat
     2581 aaaggttcta attgataaag attgtagaat taaaagggac atagttccga ctgatataga
     2641 ttatcatgta agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg aattttggaa
     2701 tgtaacacct tatgttatta aaggattgtg ccgtaaggaa attttatttg ctattgatca
     2761 ttttaatcag attgttcgcc atgagctgct gagaatgata tcatggaagg tcggcatcga
     2821 aacaggcttt aaattaagtg taggcaagaa ctataagttt attgaaaggt atatatccga
     2881 ggatttgtgg gagaaacttt tgtccaccta ccggatggat tcctatgaaa acatatggga
     2941 agcattattt ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct
     3001 tcattatgcc tatccggagt atgataggaa tataacaaaa tataccaggg acatgtataa
     3061 aaaatacact ggtaaaaccg gctgcctgga tagcacatat gccgctgata tagaagagag
     3121 gcgggaacag tgattacaga aatgaaagca gggcacctga aagatatcga taaacccagc
     3181 gaaccatttg aggtgatagg taagattata ccgaggtatg aaaacgagaa ttggaccttt
     3241 acagaattac tctatgaagc gccatattta aaaagctacc aagacgaaga ggatgaagag
     3301 gatgaggagg cagattgcct tgaatatatt gacaatactg ataagataat atatctttta
     3361 tatagaagat atcgccgtat gtaaggattt cagggggcaa ggcataggca gcgcgcttat
     3421 caatatatct atagaatggg caaagcataa aaacttgcat ggactaatgc ttgaaaccca
     3481 ggacaataac cttatagctt gtaaattcta tcataattgt ggtttcaaaa tcggctccgt
     3541 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
     3601 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgtggttct gttgcaaagt
     3661 tttaaataaa gaataaaatc cc
//
LOCUS       DAFCNS010000266         2790 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.156, whole genome shotgun sequence.
ACCESSION   DAFCNS010000266 DAFCNS010000000
VERSION     DAFCNS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.156"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1311
                     /locus_tag="LNE23_003034"
     CDS             <1..1311
                     /locus_tag="LNE23_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HBK6932799.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1441..2613)
                     /locus_tag="LNE23_003035"
     CDS             complement(1441..2613)
                     /locus_tag="LNE23_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBK6932800.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          979 a          354 c          490 g          967 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat gaaaaataat
     1381 aaaggaagtg agtcaagtcc agactcctgt gtaaaatgct atacaatgtt tttaccattt
     1441 ctacttatca aaattgatgt attttcttga agaataaatc cattcatcat gtaggtccat
     1501 aagaacggct ccaattaagc gattggctga tgtttgattg gggaagatgc gaataatctt
     1561 ttctcttctg cgtacttctt gattcagtcg ttcaattaga ttggtactct ttagtcgatt
     1621 gtgggaattt ccttgtacgg tatattgaaa ggcgtcttcg aatccatcat ccaatgatgc
     1681 gcaagctttt gaatattttg gttgatcgat ataatcatga atcaatcgat ttttagcctc
     1741 acgcgctaag ttaatatctg tgaacttaaa aattccttta acagcttctc tgaaagattt
     1801 tgaatttttt ttaggaatgg tggtaaagat atttcttagg aagtgaactt ggcatctttg
     1861 ccaacttacg ttggtgaagg attttctaat ggcagagact aatcctttgt gcgcatcaga
     1921 aataacgagt tccgtacctt gtaaaccgcg ttcttttagg tattcaaaaa atgttgtcca
     1981 ggtctcttcg ctttcgccac tttgaatcat gaagccgata atttcacggt cgccatcttt
     2041 ggttattcca atcgctatat gacagctttt tgagagtact cgattttctt ctcgtacttt
     2101 tatatagagt acatcggtca ttaagtaagg ataatttttt tctgataata aacgattctg
     2161 ccactcgtta accataggtt ctagctgttc tgttaagcta gaaacgaagg acttagagac
     2221 ggatttacca caaagttctt ccacaatttt tgatacttta cgagttgaaa cgcctgatac
     2281 atacatttcc aacattgaag ccatgagggc tttttcgttt cgttgataac gttcaaacac
     2341 tgtgggtgaa aaatggccat cacgtgttct gggtactttt aattctagcg tgcctacacg
     2401 tgtcgtaaag ctgcgctcat aatagccatt tcgttgactt tgtcggtttt ctgttcgttc
     2461 atattctttt gcttgaatat attctgttcg ttgattttcc attagttgat taaataccgt
     2521 tgttaaaata tttttagaaa cgtcatcctt tacagaatat tcaataatgc tttgaatctc
     2581 ttcgcttttc agtgtaaaat gtacttgggt catgtaaaag tcctcctggg tatgtttttg
     2641 tcgttaaaaa cattgtaccg taaaaggact gttatatggc ctttttactt ttacacaatt
     2701 atacggactt tatctatata aaggcgctgt cagagttttt tgacttagaa catacattca
     2761 ttcaagagac tttaaacaag ctagcagaac
//
LOCUS       DAFCNS010000267         1880 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.171, whole genome shotgun sequence.
ACCESSION   DAFCNS010000267 DAFCNS010000000
VERSION     DAFCNS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.171"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..1851
                     /gene="tet(M)"
                     /locus_tag="LNE23_003036"
                     /pseudo
     CDS             22..1851
                     /gene="tet(M)"
                     /locus_tag="LNE23_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
BASE COUNT          651 a          293 c          392 g          544 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggtt ctgttgcaaa
     1861 gttttaaata aagaataaaa
//
LOCUS       DAFCNS010000268         1880 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.172, whole genome shotgun sequence.
ACCESSION   DAFCNS010000268 DAFCNS010000000
VERSION     DAFCNS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.172"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..1851
                     /gene="tet(M)"
                     /locus_tag="LNE23_003037"
                     /pseudo
     CDS             22..1851
                     /gene="tet(M)"
                     /locus_tag="LNE23_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
BASE COUNT          650 a          293 c          393 g          544 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggtt ctgttgcaaa
     1861 gttttaaata aagaataaaa
//
LOCUS       DAFCNS010000269         1781 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.175, whole genome shotgun sequence.
ACCESSION   DAFCNS010000269 DAFCNS010000000
VERSION     DAFCNS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.175"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..416
                     /locus_tag="LNE23_003038"
     CDS             24..416
                     /locus_tag="LNE23_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6932801.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="LNE23_003039"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="LNE23_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBK6932802.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DAFCNS010000270         1149 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.204, whole genome shotgun sequence.
ACCESSION   DAFCNS010000270 DAFCNS010000000
VERSION     DAFCNS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.204"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..77
                     /locus_tag="LNE23_003040"
     CDS             12..77
                     /locus_tag="LNE23_003040"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBK6932803.1"
                     /translation="MFYTIIWTLSKQTKNSDYADK"
     gene            202..939
                     /gene="erm(B)"
                     /locus_tag="LNE23_003041"
     CDS             202..939
                     /gene="erm(B)"
                     /locus_tag="LNE23_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBK6932804.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          200 c          185 g          343 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc gaaacagact aaaaacagtg
       61 attacgcaga taaataaata cgttagatta attcctacca gtgactaatc ttatgacttt
      121 ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt ttatagatgt
      181 aatcacttca ggagagatta catgaacaaa aatataaaat attctcaaaa ctttttaacg
      241 agtgaaaaag tactcaacca aataataaaa caattgaatt taaaagaaac cgataccgtt
      301 tacgaaattg gaacaggtaa agggcattta acgacgaaac tggctaaaat aagtaaacag
      361 gtaacgtcta ttgaattaga cagtcatcta ttcaacttat cgtcagaaaa attaaaactg
      421 aatactcgtg tcactttaat tcaccaagat attctacagt ttcaattccc taacaaacag
      481 aggtataaaa ttgttgggaa tattccttac catttaagca cacaaattat taaaaaagtg
      541 gtttttgaaa gccgtgcgtc tgacatctat ctgattgttg aagaaggatt ctacaagcgt
      601 accttggata ttcaccgaac actagggttg ctcttgcaca ctcaagtctc gattcagcaa
      661 ttgcttaagc tgccagcgga atgctttcat cctaaaccaa aagtaaacag tgtcttaata
      721 aaacttaccc gccataccac agatgttcca gataaatatt ggaagctata tacgtacttt
      781 gtttcaaaat gggtcaatcg agaatatcgt caactgttta ctaaaaatca gtttcatcaa
      841 gcaatgaaac acgccaaagt aaacaattta agtaccatta cttatgagca agtattgtct
      901 atttttaata gttatctatt atttaacggg aggaaataat tctatgagtc gcttttttaa
      961 atttggaaag ttacacgtta ctaaagggaa tggagataaa ttattagata tactactgac
     1021 agcttccaag aagctaaaga ggtccctagc gcctacgggg aatttgtatc gataaggggt
     1081 acaaattccc actaagcgct cgggacccct tgtaggaaaa tgtcctaagt gtggcaacaa
     1141 tattgtatt
//
LOCUS       DAFCNS010000271         1126 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1321
            SAMN22630969-rid13287903.guided.205, whole genome shotgun sequence.
ACCESSION   DAFCNS010000271 DAFCNS010000000
VERSION     DAFCNS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22630969
            Sequence Read Archive: SRR16608659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171164.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171164.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 06:14:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 33 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 7 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22630969-rid13287903.guided.205"
                     /isolate="V1321"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-01-14"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            87..146
                     /gene="ermCL"
                     /locus_tag="LNE23_003042"
     CDS             87..146
                     /gene="ermCL"
                     /locus_tag="LNE23_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HBK6932805.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            194..928
                     /locus_tag="LNE23_003043"
     CDS             194..928
                     /locus_tag="LNE23_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HBK6932806.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          442 a          158 c          159 g          367 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaacca gcttgtgcta taattattct
       61 aatttcataa aaaggagaat taatatatgg gcatttttag tatttttgta atcaacacag
      121 ttcattatca accaaacaaa aaataagtgg ttataatgga ttgttaatat aaacctcata
      181 ggagtgggtt ataatgaaca aaaaaaatat aaaagatagt caaaacttta ttacttcaaa
      241 gcatcatata aatgaaattt tgagaaatgt acatttaaat acaaatgata atattattga
      301 gattggttca gggaaaggtc atttcacgtt tgaattagct aaaaggtgta attatgtaac
      361 cgccattgaa atagatccta aattatgtag gataactaaa aacaaactta ttgaatatga
      421 gaacttccag gttatcaata aagatatttt acaatttaag tttcctaaaa ataagtcata
      481 taagatatat ggaaatatac cctacaatat aagtacagat ataattcgaa aaattgtttt
      541 tgaaagcaca gctacagaaa gttatttaat agtggaatat ggatttgcta aaaggttgct
      601 aaatacaaat cgttcactag cactattttt aatgacagaa gttgatatat ccatattaag
      661 taaaatccct agagaatact ttcatccaaa acctagagtt aatagctcgt taattgtatt
      721 aaaaagacac ccttcaaaaa tatcactcaa agatagaaaa caatatgaaa attttgttat
      781 gaaatgggtt aacaaagaat acataaaact attttccaaa aaccaatttt atcaagcctt
      841 aaaatatgca agaattgacg atttaaacaa tattagcttt gaacaattct tgtctctttt
      901 caatagctat aaattattta atagataagt taaggggtgc ataaactgca tcccttaact
      961 tatcttattt tgcttaaaca atcttggccc gtaaactgca taactcatat gtaaatcgct
     1021 cctttttagg tggcacaaat gtgaggcatt ttcactattt ccggcaatta cttccatgta
     1081 aagtataacc cactatactt tgcaagcaaa gtgtgggctc gccgag
//
